LOCUS       DAVBCB010000001        83357 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVBCB010000001 DAVBCB010000000
VERSION     DAVBCB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 83357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83357
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.001"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..183
                     /locus_tag="VQU28_000001"
     CDS             <1..183
                     /locus_tag="VQU28_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2475442.1"
                     /translation="LTVEDKAYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFG
                     LRMNLIAGIINHELGF"
     gene            251..499
                     /locus_tag="VQU28_000002"
     CDS             251..499
                     /locus_tag="VQU28_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475443.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRIYSGNLILQELITVNGAYKATYIGELSSN"
     gene            630..1802
                     /locus_tag="VQU28_000003"
     CDS             630..1802
                     /locus_tag="VQU28_000003"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HET2475444.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENADSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            1802..3076
                     /locus_tag="VQU28_000004"
     CDS             1802..3076
                     /locus_tag="VQU28_000004"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HET2475445.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLEFANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLIGILSNYATDSL
                     VTASCRMAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VAFSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(3112..4077)
                     /locus_tag="VQU28_000005"
     CDS             complement(3112..4077)
                     /locus_tag="VQU28_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2475446.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQTIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(4058..5512)
                     /locus_tag="VQU28_000006"
     CDS             complement(4058..5512)
                     /locus_tag="VQU28_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2475447.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKSQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            5889..7772
                     /locus_tag="VQU28_000007"
     CDS             5889..7772
                     /locus_tag="VQU28_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2475448.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYALADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVSKGDAVIE
                     VKI"
     gene            7921..8808
                     /locus_tag="VQU28_000008"
     CDS             7921..8808
                     /locus_tag="VQU28_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2475449.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGTSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(9128..9745)
                     /locus_tag="VQU28_000009"
     CDS             complement(9128..9745)
                     /locus_tag="VQU28_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HET2475450.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(10160..10348)
                     /locus_tag="VQU28_000010"
                     /pseudo
     CDS             complement(10160..10348)
                     /locus_tag="VQU28_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(10858..10938)
                     /locus_tag="VQU28_000011"
                     /pseudo
     CDS             complement(10858..10938)
                     /locus_tag="VQU28_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            11054..11947
                     /locus_tag="VQU28_000012"
     CDS             11054..11947
                     /locus_tag="VQU28_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475451.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            11944..13143
                     /locus_tag="VQU28_000013"
     CDS             11944..13143
                     /locus_tag="VQU28_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2475452.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLEQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILDHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(13496..14263)
                     /locus_tag="VQU28_000014"
     CDS             complement(13496..14263)
                     /locus_tag="VQU28_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475453.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(14280..14975)
                     /locus_tag="VQU28_000015"
     CDS             complement(14280..14975)
                     /locus_tag="VQU28_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475454.1"
                     /translation="MSLLVFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(14980..15345)
                     /locus_tag="VQU28_000016"
     CDS             complement(14980..15345)
                     /locus_tag="VQU28_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2475455.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMIH
                     FGYEKEELPGVVSDYIKGV"
     gene            15485..15958
                     /gene="nrdR"
                     /locus_tag="VQU28_000017"
     CDS             15485..15958
                     /gene="nrdR"
                     /locus_tag="VQU28_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HET2475456.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            15959..17128
                     /locus_tag="VQU28_000018"
     CDS             15959..17128
                     /locus_tag="VQU28_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HET2475457.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFNPKEATIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEEAVLRIRER
                     GQKTKTQKQNQTAPAKTNIPKWSNPDYKNETSEETRLELERKKQELLARLEKGGD"
     gene            17129..18025
                     /gene="dnaI"
                     /locus_tag="VQU28_000019"
     CDS             17129..18025
                     /gene="dnaI"
                     /locus_tag="VQU28_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HET2475458.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            18022..18735
                     /locus_tag="VQU28_000020"
     CDS             18022..18735
                     /locus_tag="VQU28_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HET2475459.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            18749..20059
                     /gene="der"
                     /locus_tag="VQU28_000021"
     CDS             18749..20059
                     /gene="der"
                     /locus_tag="VQU28_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HET2475460.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            20150..20485
                     /locus_tag="VQU28_000022"
     CDS             20150..20485
                     /locus_tag="VQU28_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475461.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            20625..20960
                     /locus_tag="VQU28_000023"
     CDS             20625..20960
                     /locus_tag="VQU28_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475462.1"
                     /translation="MEYLFKMIILLPCFYFFSWIEKANRDSKIFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            21145..21936
                     /locus_tag="VQU28_000024"
     CDS             21145..21936
                     /locus_tag="VQU28_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475463.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAIITYFFGTFSPLEWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            21929..22570
                     /locus_tag="VQU28_000025"
     CDS             21929..22570
                     /locus_tag="VQU28_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475464.1"
                     /translation="MFKNFNNIWLKRKIVLLLRIVLMMILINYLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLVLLSLLVMLIVSRLEEKFKKG"
     gene            22574..23224
                     /locus_tag="VQU28_000026"
     CDS             22574..23224
                     /locus_tag="VQU28_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2475465.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            23235..23522
                     /locus_tag="VQU28_000027"
     CDS             23235..23522
                     /locus_tag="VQU28_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475466.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     MQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            23668..26181
                     /gene="secA"
                     /locus_tag="VQU28_000028"
     CDS             23668..26181
                     /gene="secA"
                     /locus_tag="VQU28_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HET2475467.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMATLTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQAMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            26262..27293
                     /locus_tag="VQU28_000029"
     CDS             26262..27293
                     /locus_tag="VQU28_000029"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2475468.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            27295..28326
                     /locus_tag="VQU28_000030"
     CDS             27295..28326
                     /locus_tag="VQU28_000030"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HET2475469.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            28365..28733
                     /locus_tag="VQU28_000031"
     CDS             28365..28733
                     /locus_tag="VQU28_000031"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HET2475470.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            28723..29826
                     /locus_tag="VQU28_000032"
     CDS             28723..29826
                     /locus_tag="VQU28_000032"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HET2475471.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLALPYDLIPALTLESALVHIKTVPAGACMGYGATYQTDSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            29845..31860
                     /gene="recG"
                     /locus_tag="VQU28_000033"
     CDS             29845..31860
                     /gene="recG"
                     /locus_tag="VQU28_000033"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HET2475472.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     IKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            32176..33156
                     /locus_tag="VQU28_000034"
     CDS             32176..33156
                     /locus_tag="VQU28_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HET2475473.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            33961..34188
                     /locus_tag="VQU28_000035"
     CDS             33961..34188
                     /locus_tag="VQU28_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475474.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            34476..37373
                     /gene="nanA"
                     /locus_tag="VQU28_000036"
     CDS             34476..37373
                     /gene="nanA"
                     /locus_tag="VQU28_000036"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HET2475475.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGNRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NRRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            38389..38841
                     /locus_tag="VQU28_000037"
     CDS             38389..38841
                     /locus_tag="VQU28_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2475476.1"
                     /translation="MIITKISHLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVQLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            38860..40197
                     /locus_tag="VQU28_000038"
     CDS             38860..40197
                     /locus_tag="VQU28_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2475477.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            40276..41160
                     /locus_tag="VQU28_000039"
     CDS             40276..41160
                     /locus_tag="VQU28_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2475478.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            41160..41993
                     /locus_tag="VQU28_000040"
     CDS             41160..41993
                     /locus_tag="VQU28_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2475479.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAIFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            42011..44104
                     /gene="nanB"
                     /locus_tag="VQU28_000041"
     CDS             42011..44104
                     /gene="nanB"
                     /locus_tag="VQU28_000041"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HET2475480.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISPLLLDKLSGESQTVVMKFKADEPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKRSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNREESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTSGELTYLISDDSGQTWKKSSASIPFKNATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAITELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            44116..45219
                     /locus_tag="VQU28_000042"
     CDS             44116..45219
                     /locus_tag="VQU28_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2475481.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            45393..46091
                     /locus_tag="VQU28_000043"
     CDS             45393..46091
                     /locus_tag="VQU28_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2475482.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            46110..47627
                     /locus_tag="VQU28_000044"
     CDS             46110..47627
                     /locus_tag="VQU28_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2475483.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFLGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            47752..49080
                     /locus_tag="VQU28_000045"
     CDS             47752..49080
                     /locus_tag="VQU28_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2475484.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            49332..50198
                     /locus_tag="VQU28_000046"
     CDS             49332..50198
                     /locus_tag="VQU28_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2475485.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            50214..51053
                     /locus_tag="VQU28_000047"
     CDS             50214..51053
                     /locus_tag="VQU28_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2475486.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            51326..51778
                     /locus_tag="VQU28_000048"
     CDS             51326..51778
                     /locus_tag="VQU28_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2475487.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQVENNQFEHHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEATDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPTGYAGMEEKVRKYLFKILID"
     gene            51875..52219
                     /locus_tag="VQU28_000049"
     CDS             51875..52219
                     /locus_tag="VQU28_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475488.1"
                     /translation="MVSSKHAARASFLDGQEITVDEMSWIINGIVNALIGRYIKLGTY
                     AAKYGIGIARSILSRAAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            52503..53147
                     /locus_tag="VQU28_000050"
     CDS             52503..53147
                     /locus_tag="VQU28_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2475489.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            53316..54233
                     /locus_tag="VQU28_000051"
     CDS             53316..54233
                     /locus_tag="VQU28_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HET2475490.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKESFL"
     gene            54251..55135
                     /locus_tag="VQU28_000052"
     CDS             54251..55135
                     /locus_tag="VQU28_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2475491.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(55151..56002)
                     /locus_tag="VQU28_000053"
     CDS             complement(55151..56002)
                     /locus_tag="VQU28_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2475492.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANRVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            56202..58259
                     /gene="pbp2b"
                     /locus_tag="VQU28_000054"
     CDS             56202..58259
                     /gene="pbp2b"
                     /locus_tag="VQU28_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HET2475493.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTKRQLADYYLADPEIYKKTVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            58270..58866
                     /gene="recR"
                     /locus_tag="VQU28_000055"
     CDS             58270..58866
                     /gene="recR"
                     /locus_tag="VQU28_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HET2475494.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            59042..60085
                     /locus_tag="VQU28_000056"
     CDS             59042..60085
                     /locus_tag="VQU28_000056"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HET2475495.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKITMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            60169..61542
                     /locus_tag="VQU28_000057"
     CDS             60169..61542
                     /locus_tag="VQU28_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HET2475496.1"
                     /translation="MKLTIHEVAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGVRDGHDFIERAFENGAAATLSEKEVANHPYILVDDVLTAFQQLAAYYLEKTAVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPTDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQVLDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            61529..62140
                     /locus_tag="VQU28_000058"
     CDS             61529..62140
                     /locus_tag="VQU28_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2475497.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRDLLSEML
                     NQASDLDSEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDPLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            62231..62935
                     /locus_tag="VQU28_000059"
     CDS             62231..62935
                     /locus_tag="VQU28_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HET2475498.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            63154..64527
                     /gene="ftsA"
                     /locus_tag="VQU28_000060"
     CDS             63154..64527
                     /gene="ftsA"
                     /locus_tag="VQU28_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HET2475499.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENIIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVESVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            64544..65803
                     /gene="ftsZ"
                     /locus_tag="VQU28_000061"
     CDS             64544..65803
                     /gene="ftsZ"
                     /locus_tag="VQU28_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HET2475500.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETVELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            65808..66479
                     /locus_tag="VQU28_000062"
     CDS             65808..66479
                     /locus_tag="VQU28_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HET2475501.1"
                     /translation="MNVKENTELVFREVAEASLSAHRESGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            66489..67028
                     /locus_tag="VQU28_000063"
     CDS             66489..67028
                     /locus_tag="VQU28_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HET2475502.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            67028..67291
                     /locus_tag="VQU28_000064"
     CDS             67028..67291
                     /locus_tag="VQU28_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HET2475503.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            67288..67972
                     /locus_tag="VQU28_000065"
                     /pseudo
     CDS             67288..67972
                     /locus_tag="VQU28_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
     gene            67981..68769
                     /gene="divIVA"
                     /locus_tag="VQU28_000066"
     CDS             67981..68769
                     /gene="divIVA"
                     /locus_tag="VQU28_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HET2475504.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            69117..71909
                     /locus_tag="VQU28_000067"
     CDS             69117..71909
                     /locus_tag="VQU28_000067"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HET2475505.1"
                     /translation="MKLKDTLNLGKTEFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYANFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFEAEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEEPAPEAALSFEDVVFTVERAAGEVCDRCRRIDPTTAERSYHAVICDHCASI
                     VEENFADAVAEGFEEK"
     gene            72319..72681
                     /locus_tag="VQU28_000068"
     CDS             72319..72681
                     /locus_tag="VQU28_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475506.1"
                     /translation="MKSSGIVGLVGGILLLICPFVLLGTAVNTAATTLNGGATAGAFS
                     GVALLLNALKIANLVLGIIAIVYYKGDKHVGAAPSVLMIVSGEVSLIPFLGWVGGILA
                     IIGGSLFLATLKKFKSEE"
     gene            73216..73908
                     /locus_tag="VQU28_000069"
     CDS             73216..73908
                     /locus_tag="VQU28_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HET2475507.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            73961..76372
                     /locus_tag="VQU28_000070"
     CDS             73961..76372
                     /locus_tag="VQU28_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2475508.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     RKAFASFPDDLLIQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQALEELFFQYGRYLLISSSRDCPDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TEVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYSGNLFSY
                     KGQEYIEAARAILNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMAEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            76622..77323
                     /locus_tag="VQU28_000071"
     CDS             76622..77323
                     /locus_tag="VQU28_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475509.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            77325..80025
                     /locus_tag="VQU28_000072"
                     /pseudo
     CDS             77325..80025
                     /locus_tag="VQU28_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(80121..80612)
                     /gene="tpx"
                     /locus_tag="VQU28_000073"
     CDS             complement(80121..80612)
                     /gene="tpx"
                     /locus_tag="VQU28_000073"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HET2475510.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(80735..81664)
                     /gene="psaA"
                     /locus_tag="VQU28_000074"
     CDS             complement(80735..81664)
                     /gene="psaA"
                     /locus_tag="VQU28_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HET2475511.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(81696..82544)
                     /locus_tag="VQU28_000075"
     CDS             complement(81696..82544)
                     /locus_tag="VQU28_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2475512.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(82541..83263)
                     /locus_tag="VQU28_000076"
     CDS             complement(82541..83263)
                     /locus_tag="VQU28_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2475513.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
BASE COUNT        25210 a        15195 c        18403 g        24549 t
ORIGIN      
        1 ctaacagttg aagataaagc ctataaccat gcgctatcta aggagagaag caaggttgag
       61 aacatctttg ccaaagtaaa aacgtttaaa atgttttcaa caacctatcg aaatcatcgt
      121 aaacgcttcg gattacgaat gaatttgatt gctggcatta tcaatcatga actaggattc
      181 tagttttgca ggaagtctac tactttcctt attgtctgta aatctactga ccttgttgtt
      241 tatcccagtc atggtttcta gttcgggctc agagtttcaa agtggatggc aagagcatca
      301 attgattgct gagaaggtta gtaaaacact tgataagaca tttgataagg atgtcagaga
      361 aattccgacc agtcagtttt atcaaaaatt tgtagatgag atgggaagga tttactcagg
      421 aaatttgatc ctccaggagc tgataactgt gaatggagct tataaagcta cttatatcgg
      481 tgaactctct agcaactaat cattctttaa aatgatgacc aataagaggc ttgatttggt
      541 gaatcaagtc ttttttctct cttttactgc catttgtgat aaaatagtat agaacgattt
      601 tttacatgaa tgataaaaca gaggtaaata tgacaatcgg tattgataag attggttttg
      661 cgaccagtca atatgtcttg aaattacaag acttagcaga agcgagggga attgaccctg
      721 aaaaattaag taaaggactc ttactcaagg aattgagtat tgcgccccta actgaggaca
      781 tcgtgacctt ggcggccagt gctagtgact ctattttaac tgagcaagaa agacaagaag
      841 ttgacatggt cattgtggct accgagtcag gaattgacca gagtaaggct gcggccgtct
      901 ttgtgcatgg cttgctgggc atccagccct ttgctcgtag tttcgagatt aaagaagcct
      961 gctatggggc gacagctgcc ctccattatg ccaaattgca tgtggaaaat tctccggagt
     1021 ccaaggtctt ggtcattgcc agtgatattg ccaaatatgg tattgaaact ccgggagaac
     1081 caactcaagg tgctggaagt gtagctatgt tgattacaca aaatccacgc atgatggcct
     1141 ttaataatga caatgtagct cagacccgtg acatcatgga tttctggcga ccaaattact
     1201 cgacaactcc ttatgtaaat ggtgtctatt ctacccaaca atacttggat agtttgaaaa
     1261 cgacttggct tgaatatcaa aaacgctacc agcttactct ggatgatttt gcggctgttt
     1321 gtttccactt gccttatcct aaattagcgc taaaaggctt gaaaaaaatc atggataaga
     1381 gcctgcctca agagaaaaaa gacctcttac aaaagcattt tgaccagtct attctctaca
     1441 gtcaaaaggt ggggaatatc tacacaggtt cacttttcct tggacttttg tctctcttgg
     1501 aaaatgcaga tagcttgaaa gctggggata aaatcgccct ttatagttac ggaagtggag
     1561 ctgtggctga gttcttcagt ggtgaattgg ttgaaggata tgaagcttat ttggataaag
     1621 accgcttgaa caagctcaac caacgaactg ccttatccgt tgcagactat gaaaaggtct
     1681 tttttgagga agtaaacttg gatgaaacaa actctgccca gtttgctggc tatgaaaatc
     1741 aagattttgc cttggttgaa attctcgacc accaacgccg ttatagcaag gttgaaaaat
     1801 aatgaagata agttggaatg gattttctaa aaaatcatac caagagcgcc tcgagctgtt
     1861 aaaagctcag gcgctcctta gtcctgagag acaagctagt ctggagaagg atgaacagat
     1921 gagtgtgact gtggcagacc agctgagtga gaatgtagtg ggaacttttt ctctgcctta
     1981 ttcgctggtt ccggaggtac ttgtcaacgg tcaggaatac accgttccct atgtgacaga
     2041 agaaccctct gtggttgcgg cggccagcta tgccagcaaa atcatcaagc gtgcaggtgg
     2101 ttttactgca caagtccatc agcgccagat gattgggcag gtagcccttt atcaagttgc
     2161 taatcctaaa ctagcgcaag agaagattgc cagcaagaaa gcggagctct tggagtttgc
     2221 caatcaagcc tatccttcta tcgttaaacg tggaggtggg gcgcgtgatc tgcatgtcga
     2281 gcagataaaa ggcgaaccag actttctcgt tgtttatatt catgtcgata cccaggaagc
     2341 catgggtgcc aatatgctca acaccatgct ggaagccttg aaaccagtct tagaagaact
     2401 cagtcaggga cagagtctca taggaatcct gtccaactac gcgaccgatt ctctggtgac
     2461 tgcaagctgt cgcatggcct ttcgctactt gagccgccaa aaggatcaag gacgagagat
     2521 tgcggagaaa attgcgttgg ctagtcagtt tgcgcaggct gatccttacc gagctgctac
     2581 tcataataaa ggaattttta atggtattga tgcgattttg attgccactg gtaatgactg
     2641 gcgtgccatc gaagctgggg cccatgcctt tgccagtcga gatggacgct atcaaggtct
     2701 tagttgctgg acgctggacc ttgaaagaga agaattggtc ggtgagatga ccctgcccat
     2761 gcctgtagcg actaagggtg gctctatcgg cctcaaccca cgtgtagctt tcagtcatga
     2821 tctactagga aatccttctg ccagagaatt agcccagatt atcgtgtcca tcggtcttgc
     2881 ccaaaatttt gcagccctca aagctttggt aagtacgggc atccagcaag gccacatgaa
     2941 actacaggcc aaatccctag ctctcctagc tggggctagt gaatctgaag ttgctcccct
     3001 agtagagcgc ctcatctcag ataaaacctt taacctagag acagcccagc gctatctcga
     3061 aaatttaaga tcataaaaaa ctcagacgaa tcggtctgag ttttcttgtg tttaaatact
     3121 ttttcctggt aatagtgtaa ctggtaagaa gtaaccagtt gtggcaactt ccttgccttc
     3181 aatcttttgc aagagaagat caatagtgag acaagcaatc tcttccaaag gttgcttgat
     3241 agtagccaat tgagggtagt aattttcgat aaagtaggtc ccatcatagc cgatgacctt
     3301 gagctctttt gggacagaaa tgcccaattc ttgagcgatt ttaatgacca gaatagctgt
     3361 caaatcatcc gaagcaaaaa tggcatctgg tttttcccgg gtcaagatat tcttgatttc
     3421 catttctttt ctgacgggag aaaagtcact ggaaacattg ataataggag cttttgggag
     3481 tacggatgca aaaccagcgt ggcgtagtcc ggttggcgaa ttggaattgt cattccctgt
     3541 aatcatgatg atagtctggg cacctgtctt gaccaaggtt tgggcagcaa gaaccccacc
     3601 agcatagttg tcagaggaga cgacagggat gtctggcgat aggtttcggt caaaggaaat
     3661 aatcggcgct gtcacacgat tgtagtcttc gattcctagg ttgtgactac cagaaatgat
     3721 gccgtccacc tgattggctt ccaacatttc gatgtattcg cgttccttct cagaatcatg
     3781 ttcactgttg cagatgatgg tcttgtaacc atttttgaag agttggtgtt ccaatttatc
     3841 aatcaattct gcatagaaaa cattggaaat attagggaaa atcaagccga ttaacttagc
     3901 tgattttcct tgcagactac gagccaggtt gttgggttta tagcccaatt ctcgcatggc
     3961 ttcattgact ttttggatgg ttttctcaga tagataccct tttttattga taacccgaga
     4021 aacggtagta ggactgacgc ctgcaagttt ggcgacatca gttagttttg cgaccataat
     4081 ctaattcata gtaagttcca gttgggtttc cagatttgat caggatacca ttttggtccg
     4141 catgtgggaa gacacgacca gaaaatactt tttctccttt attgatgaaa atttcaaaga
     4201 cagagttatc gatgaagatt gtagcagtag tagcctgatt ctcgataggg caagaacgag
     4261 ttgtcccaaa ttcttgggca tactgttctc cagcctggct acgatccact gtcacttgac
     4321 cgtttacaag gtcaaagttg attgaaagtc ccttaccttc tttatcagca agtaagacaa
     4381 tctcgctctg gctattagct tccaagttga gttcaagttc gtaagtgttc ttggtttggg
     4441 aacggtttga gaaggcttct tcagaagcac gaaggtcctt aatagcagcg actggatact
     4501 ggtagagctt gtcgtctttg atagtgagtt ccttgaccaa agagaaggtt ccttggtggt
     4561 caaaacggtc agatgggtaa gaaacatctg gcaaaccaag ccagctaact gctagagcac
     4621 gcccatcagg agcgttgaag gcttgagttg cataggcttc gaaaccgtaa tccatgtttt
     4681 gaagttgaga cacatctacc attttggcat ttttagggtc aaaggaagcc ccaatcttat
     4741 acatatttgg aaagatatta tcgtagtcta gaactttctt atccaatcct tgtggacagt
     4801 agagaaggac aggatgttcc tctacaaaga ccagattagg acattccatc atgtaggcag
     4861 tacggtcgtt agcaaagtca aggtcgccaa ctgcttgcca gtttgtgtag tcgttattga
     4921 cagccttgta gagacggacg aagccttttt tctccaagtc ctgtccaccg acgacagcat
     4981 agtattgact tttaaagtta aaaatttgtg gatcgcggaa gtggtcagta gagtctgctg
     5041 gctggtcaat caagatcttg tcaatctttg taatcttacc ctccttgtcc atcaaagcac
     5101 cgatctggta tgggtgacgg atccagtttt catcgcgaac atttcctgta taaaatagga
     5161 ataagttatc gccaaattgc atggcagaac cagagtaggc accgtggcta tctaatggag
     5221 tatctggtaa aactttgatt ccagtttctt taaagtgaac caaatcatca ctttctagct
     5281 gtgcccaaga tttcaaaccg tgggctgcac caaaaggaaa attctggtaa aagaggatcc
     5341 acttgccatc aaagtaagaa aagccatttg ggtcgttgag aagtcctgtt tttggctcaa
     5401 catggtaatg agtatgccat ggagattgtg ccatattttc ctttatatgt tgaatttctt
     5461 cttgcgtcca atcttgataa agtctgtaac gacgctcagt tgtccattcc attcattcct
     5521 ccaaaaatct tatcactttt aatagtaact gttttcgata aaaaatgcaa gccttttatg
     5581 ttaaaaaaga ggaaaaactg tcaaacgatt atcataaaat aaatacagga aatcaatcaa
     5641 atttttacga aaatgtgaaa gataatgaaa taaaagactt tttaaccaag gtttcaaact
     5701 tatttttcaa ggaattcctg atgaaacagt caaaaacaat caatctaaaa cttgcccact
     5761 aagtggatag aatatttttt ctgcaaaacg cttgacatat ttttaaaaca tgataaaata
     5821 atagtcgtaa gggcgaataa aatcgctttc aaacaagaac aaaatgttat ataaggagat
     5881 ttttgcaaat gaacaatcag gaaattgcaa aaaaagtcat cgatgccttg ggcggacgtg
     5941 aaaatgtcaa tagtgttgcc cactgtgcga ctcgtctacg tgtcatggtc aaagatgaag
     6001 agaaaatcaa taaagaagtg attgagaact tggaaaaagt tcaaggtgct ttctttaact
     6061 cagggcaata ccaaattatc tttggtacag gtacagttaa caaaatgtac gatgaagttg
     6121 ttgtacttgg cttaccaaca tcatctaagg atgacatgaa agcagaagtt gctaaacaag
     6181 ggaactggtt ccaacgtgct atccgtactt tcggtgatgt tttcgttcca atcatcccag
     6241 ttatcgtagc gacaggtctc ttcatgggtg tgcgtggtct tttcaacgct cttgaaatgc
     6301 cacttccagg tgactttgca acttacacac aaatcttgac agatacagcc ttcatcatct
     6361 tgccaggttt ggttgtgtgg tcaaccttcc gtgtatttgg tggaaatcct gccgttggta
     6421 tcgttcttgg tatgatgctt gtctctggct cacttccaaa cgcttgggca gttgctcaag
     6481 gtggtgaagt aacagcgatg aacttctttg gtttcatccc tgttgttggt ttgcaaggtt
     6541 ccgttcttcc agccttcatc atcggggttg tcggagctaa atttgaaaaa gctgtccgca
     6601 aggttgttcc agatgtcatt gacctcttgg taacaccatt cgtgacactt ttggtcatgt
     6661 ctatccttgg actctttgtc attggaccag ttttccacgt tgttgaaaac tacatcctta
     6721 ttgctacaaa agcgattctt agcatgccat ttggtcttgg tggtttcttg attggtgggg
     6781 ttcaccaatt gatcgtcgtg tcaggtgtgc accacatctt caacttgctt gaagtgcaat
     6841 tacttgctgc tgaccatgct aacccattca acgctatcat cacagctgct atgacagctc
     6901 aaggtgctgc tactgttgcg gttggtgtta aaacaaaaaa tccaaaactg aaaacacttg
     6961 ctttcccggc tgctctttct gccttcctag gtattacaga gcctgctatc ttcggggtga
     7021 acttgcgctt ccgtaaacca ttcttccttt cattgattgc tggtgcaatc ggtggtggat
     7081 tggcttctat ccttggactt gctggtactg gtaatggtat caccatcatc cctggtacaa
     7141 tgctttatgt tggtaacgga caacttccac aataccttct tatggtagct gtatcatttg
     7201 cccttggttt tgctcttact tacatgtttg gttacgaaga tgaagtagac gcaactgcag
     7261 ctgcaaaaca agctgaagtg gctgaagaaa aagaagaagt tgcgccagca gctcttcaaa
     7321 atgaaacact tgtaactcct atcgtcggtg atgttgtcgc tcttgctgat gtcaatgacc
     7381 cagtcttctc aagtggagct atgggacaag gtatcgctgt gaaaccaagt caaggcgtgg
     7441 tctatgcact agctgatgct gaagtttcaa ttgcctttcc aacagggcac gcttttggtt
     7501 tgaaaacaag aaatggtgct gaagttttga ttcacgttgg tattgacact gtatctatga
     7561 acggtgacgg ttttgaagca aaagttgctc aaggtaataa ggtgaaagct ggcgatgttc
     7621 ttggaacatt tgactcaaac aaaatcgctg cagctggact tgatgataca acaatggtta
     7681 tcgttacaaa tacagctgac tacgcttcag tagctccagt cgcaacaggt tcagtttcga
     7741 agggggatgc tgtgatcgaa gtgaaaatct aatcaatcct ctctaatgtg aaaacgaaca
     7801 aatgacatgt ttgttcgttt ttattagata gtaagattta cagaggaaaa tctaaaaaat
     7861 agagacattt agactttcga agtatgctat aataaagaaa ataaaaacaa gaggtttatc
     7921 atgacaaaat tatatggaag cttggaagcg ggcggtacaa agtttgtctg tgctgtcggt
     7981 gatgaaaact ttaacgttgt agaaaaaaca caatttccaa caacaactcc aatcgaaaca
     8041 atcgataaaa ccattgagtt cttctcaaaa ttcgataacc ttgctggtct tgcagttggt
     8101 tcatttgggc cgattgatat tgacaaaaac tcaaaaactt atggctttat cacgacgact
     8161 ccaaaaccaa actgggcaaa tgtggacttg cttggtgccc ttcgtcgcgc cctaaacgtg
     8221 ccaatgtact tcacaacaga cgtaaacagc tctgcttacg gtgaaatggt tgctcgtaac
     8281 aatgctggtg gtcgtatcga aaacttggtt tactacacaa tcggtacagg tatcggtgca
     8341 ggcgtcatcc aacgtggtga gtttatcggt ggtgtgggtc accctgaaat gggtcattat
     8401 tatgttgcta gacacccaat ggatattgaa aaagagttta agggtgtttg tcctttccat
     8461 aagggatgtc tagaaggtta tgcagctggt acaagtttgg aagctcgtac aggtgtacgt
     8521 ggggaaacta ttgaactcaa caaccctgtt tgggatgttc aagcctacta tatcgctcaa
     8581 gctgcggtta atgcgacagt gactttccgc ccagacgtga ttgtctttgg tggaggggtc
     8641 atggctcaac aacatatgct ggaccgtgtc cgtgagaaat ttacatctct tcttaatggt
     8701 tacctaccag taccagatgt gcgtgactat atcgtgacgc cagcagtcgc aggaaatggt
     8761 tctgccacac ttgggaactt tgttcttgca aaagaagttt caaaataaag cacaaaatcc
     8821 gcttgggaaa ccaaacggat tttgtggttg aaaacaatat ttttgagcct ttccttaagt
     8881 gattacggac ggtagcgact ttcttcgaaa ttccatacct aaactttgag cctagtgtct
     8941 caaagtttcc gaatacctga aaccattaga tttcaggtgt ttctatcacg gcggaaaggc
     9001 taggtaagtc ctcgcctcac tatcgaaaaa tatgcataat ttccatacca aagtatattt
     9061 agtatcaagc tatagttaat ctacgcttta aaaatccact tgggaaacca aacggatttt
     9121 ttacgtgtca aagcatttgc cagaaaaagt caataatata ggtcagaccg taggtataaa
     9181 ccacgagggt aaagggtttg gtcagaatga tgatagtcat gtagcgcttg aaactcatct
     9241 tggtcagggc ggccagcata cagagaaagt cagctgggct aatgggccaa atcatcataa
     9301 aaataaagaa gcggtcaaaa cgattgccct tatctagcca gccgatgtac ttgtcgtagg
     9361 tgcgcttgct gacgacagac tggacaaagg cagctccgta taggcgcact agataaaaga
     9421 taatggcaca gccaatcacg atgccgatat agttgtagat agtcccgatg atgtgcccgt
     9481 agataaagac cccagccacc gaggtcaagg cccctggaat gatagggacg acagtctgta
     9541 aaatctgtaa aaagataaag agaggtggac cccagatgcc tgcctgctgg ataaaggcag
     9601 agagggtttc cttggattgt aaaatcccag cctgataagc ccaaatacag aaaatcaaac
     9661 tcccaacccc accgacaatc gatgagatat tgataactcg ctgcagaagg ggagaaacag
     9721 ctttcatttg tttatcctgt gacatgtaaa ctcctcatag atagtatatt tctactatac
     9781 catattttgg aggagaaagg gggaatgagt cttttttgat aaggaataga aagtaaatag
     9841 cccttcgaga cttagaatag gtctgtcaac taaaggttat atgcattcta ggtttcgaat
     9901 aatcgcaatt ccataaattt aaaagtattc taagtttcga atagcttatc tgagataatt
     9961 ttgaggcatt cgcgctttcg aatagtcatt ttccctaaag tttatatgta ttctgaagct
    10021 agaaagacta aaatataaaa gtagtaagag tctctctctg atggacgtaa tttatggcta
    10081 gtatgaatcc tttgaattgt tagttaattt taatattaat cagttctgaa ataattacta
    10141 aatatagtta gatggtgagt taattattgc taaatttcaa gttcaagtta gtcatccaga
    10201 agtcttctct gggggacttg tagttcaagc attttttagg atagttgtta atccactttt
    10261 cgataaatgc gacttccttt gtagttgttt tcttagttcc ctgaggtaac catctatgaa
    10321 tgagcctgtt gtgattttca ttagatccta tagtgatgtg cataatagat atagcagatt
    10381 gaaactagaa tagtatacat ctacttctaa aacattgtta gaaatcaatt tgactgtcct
    10441 gatcaatttg tcctgttttt atttcatttt actatataat ccttagaaaa catatcggac
    10501 aagcggttga attccgttcc gtccattagt agataaagga ttactccttc ttgaaatgac
    10561 agatgaactt gtttcttata aaaatgttgt ctgaaactat tagccttttt ccagaagttt
    10621 tagaggaaga aaactttacg aggaaaaagg agttactcaa ataacaattc aggattaaaa
    10681 atagacagtt gaggagcgga aggtataaat taagtttgct actgtataat ggatttatca
    10741 ctaatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt agatatagtt
    10801 actgacttcg tcagttctat ccacaacctc aaaacagtgt tttgagctga cttcgtcagt
    10861 tttatctgca acctcaaagc tgtactttga gcagcctacg gctagcttcc tagtttgctc
    10921 tttgcttttc attgagtata aaagtgctcc aggccatatt ttacacagca atagtcttct
    10981 aaaaatggac aaaaacattg taaaggctat caaaaaggag tataatgagt gcaagaaatt
    11041 tcggaggtga aagatgctag aagtaagaag tctagagaaa agttttggct ccaagcaagt
    11101 tttgtttggt attgactttc aagcgcgacc aggtcgtatt ttgggactag tcggaaaaaa
    11161 tggtgctgga aaaacaacga ttttccatag tattttgaaa ttcctagaat atcagggaga
    11221 aatcggtctg gatggtcagg atattcgtca ggagacctat gctcggattg gttatctgcc
    11281 tgaagaacgc agtctcatgc ctaaattgac agttcttgaa caagttcgct acttggcgac
    11341 tctaaaaggc atggatgcca aagaagtcaa agaaaaactc cctcaatgga tgaagaggtt
    11401 ggaagtgaaa ggaaagctga cagataaaat caagagtctg tcaaaaggaa atcagcagaa
    11461 gattcagctc attattactc tgattcatga accagacctg attatcttgg atgagccttt
    11521 tagtggattg gacccagtta atacagaatt gctcaaacaa gtcatttttc aggaaaaaga
    11581 gcgcggagca accattatct tttctgacca tgtcatgacc aatgttgagg aactttgtga
    11641 cgatattctg atgatccgag atggccgtgt ggtcttgcac ggaccagttc aggatgtccg
    11701 caatcaatac gggaaaacgc gtctctttgt ttcaagtgaa cgaagcaagg aagaattgga
    11761 aaatcttcct catgtcaaac aggtgagctt gaccaaacaa ggcagttgga aattgatttt
    11821 ggaggatgag agcgctggaa gggaactctt cccaatcttg actcaaggtc aatatatcgc
    11881 aacatttgac caacaagcgc caactatcga tgaaatcttt aaactagaat caggagtgga
    11941 agtatgagaa atatgtgggt tgtaatcaag gaaacctatc ttcgacatgt cgagtcatgg
    12001 agtttcttct ttatggtgat ttcgccgttc ctctttttag gaatctctgt aggaattggg
    12061 catctccaag gttcttctat ggctaaaaat aataaagtgg cagtagtgac aacagtgcca
    12121 tctgtagcag aaggactgaa gaatgtaaat ggtgttaact tcgactataa agacgaagca
    12181 agtgccaaag aagcaattaa agaagaaaaa ttaaaaggtt atttgaccat tgatcaagaa
    12241 gatagtgttc taaaggcagt ttatcatggc gaaacatcgc ttgaaaatgg aattaaattt
    12301 gaggttacag gtacactcaa tgaactgcaa aatcagctta atcgttcaac tgcttccttg
    12361 tctcaagagc aggaaaaacg cttagagcag acaattcaat tcacagaaaa gattgatgaa
    12421 gccaaggaaa ataaaaagtt tattcaaaca attgcagcag gtgccttagg attctttctt
    12481 tatatgattc tgattaccta tgcgggtgta acagctcagg aagttgccag tgaaaaaggc
    12541 accaaaatta tggaagtcgt tttttctagc ataagggcaa gtcactattt ctatgcgcgg
    12601 atgatggctc tgtttctagt gattttaacg catattggga tctatgttgt aggtggtctg
    12661 gctgccgttt tgctctttaa agatttgcca ttcttggctc agtctggtat tttggatcac
    12721 ttgggagatg ctatctcact gaataccttg ctctttattt tgatcagtct tttcatgtac
    12781 gtagtcttgg cagcattcct aggatctatg gtttctcgtc ctgaggactc agggaaagcc
    12841 ttgtcgcctt tgatgatttt gattatgggt ggtttttttg gagtgacagc tctaggtgca
    12901 gctggtgaca atctcctctt gaagattggt tcttatattc cctttatttc gaccttcttt
    12961 atgccgtttc gaacgattaa tggctatgcg gggggagcag aagcatggat ttcacttgct
    13021 attacagtga tttttgcggt ggtagcaaca ggatttatcg gacgcatgta tgctagtctc
    13081 gttcttcaaa cggatgattt agggatttgg aaaaccttta aacgtgcctt atcttataaa
    13141 tagaagagcc ttgcgaaagc gaggttttta aaggtgaaaa gggtggaatc acgaacctaa
    13201 tgatgtacac attatgtaaa caggttgctt gaaaactcaa ttgtatagtg aactgaatct
    13261 ggaataggat aatattactt ctaaaacatt cttataaatt gatttgaatt ccaaaatccc
    13321 atcgttccgt tcttatttca ttttgcgata tagtgaaagg aaataagtca gctcacaaaa
    13381 aacgcataat atcaaggatt tttaacactt gatactatgc gttttctaat tttaaagact
    13441 ttttgcctgg tctcttttgt tctatgagta tgttacatat ctaggtaaaa attatttaaa
    13501 gcaaattaac cttatttctc aagatgtagt aggttccgag ataaaagata gctatgaata
    13561 tgagttcttt aacaatggct atacctgctc ccatatagaa atggtcattg agtcctacca
    13621 gtgaattgac atagaaatta gaactaagat tgaagaaaag ttcaataaat ccaatgacga
    13681 tttggatacc aatgtaggct gcaacagcga gtgctgtacg gtattcattg aaaagctgtc
    13741 caatggaaat agccaggtag atgcagagga ttcctgaaat ggtatttagt aggaaggata
    13801 tacctgtaag aaagatctga gggagatgtg tttctacaaa tgtaatcaca taagaaagag
    13861 gaatccattc tggagctgtt aaagccagaa taataacagc acttagagcc aatacagcgg
    13921 tgctaatcaa tgaccagata aaggcaccga ttagtttggc tgtgatgata tggtgttcag
    13981 aaactggcaa ggtcaaagtc agatagcctt gtcggtcgta gacactacct ttgaagcgtt
    14041 taataatcaa gaaaatggtt gaaatcgcaa gtgtaagcat caagccacca aagactgtag
    14101 ctagaaaaag tagcatagta gcctgacttt cttggtaagg tagattttta aagccttgtg
    14161 tctgtattcc gatgagggca gaaaggacta gcacggctgc gtagagggct aaataccact
    14221 tgttaacatt tttaaattcg tagtgaacta aattccaaaa cataaataat ctcctttgct
    14281 taggccttaa attcctgacg gaagagttgg tcaatggatt cacctgactc gtagcgaata
    14341 tcatctacat ttccttgacg gacgactttt ccgtctttta ggaagacaat ttcatccaag
    14401 attggctcga tatcagaaat caagtgggta gaaatcaaaa cggtagaagt tggtgagtag
    14461 ttgttgataa tggtattgag gatataagca cgggctgctg gatccacccc accaatgggt
    14521 tcatccaaaa catagagacg agcatcacgg ctcataacca aaatcagttg aaccttttct
    14581 ttgtttcctt ttgatagttt cttgagacga ctattttcat caatgcccag gtctgcaagt
    14641 agatgatgag cgcgttcaag attgaaatct ttatagaagg tcttgaagta ggttagggct
    14701 tctttgacct tcatttgctc attgagatag gtcgtatcag gcaaataagc tacaacggcc
    14761 ttggttgctg ggcttgggtc catgtcgttg atgaggacac gtccttgatc tggttgtaag
    14821 aggccattaa ttagtttaat cagggttgtt tttcctgagc cgtttggccc aagaaggccg
    14881 acaatttttc cagctggaat gtcaagagaa acattttcaa gggctggtgt tgctccatat
    14941 gatttggata cattttcaaa tactagtaat gacataggct taaactcctt taatataatc
    15001 actgactacg cctggtagtt cttctttttc atagccaaaa tggatcatgg aggaaacgaa
    15061 gtgttccaat tcttcttctg ataattgttt gcgtgactga gcgattagct ccttatcctt
    15121 agtcacaaat cgtccagttg ttcgcttgct gtagacaaat ccttctcgtt caaggtctga
    15181 taaggctctt tggatggtat tgggattgac accagcctcg ctagctagct ccctcacggt
    15241 tggaagttgt tgattgggtt ccagtgtatg ggaaacaatc tgaagcttga ttttctccat
    15301 aatctgtaaa tagatgggtt ttttgttgtc aaatgtccag gacatcttag tctcctttct
    15361 cttatccttt tgtcttaact aaataataca acaaaacaaa aaacttgtca agcaaaaaga
    15421 agaattgttt tgggaatcgc ttaaaaaatg gtataatgaa aagaaaacga taaggaggga
    15481 aaggatgcgt tgtccaaaat gtggggctac caagtcaagt gttatcgata gtcgccaagc
    15541 agaagaaggg aacaccattc gtagaagacg tgagtgcgac gaatgccaac accgttttac
    15601 aacctacgaa cgagtagaag aaagaacctt agtggttgtt aaaaaagatg gtacacggga
    15661 acaattctcc agagataaaa tctttaatgg gattatccgc tcagcccaga aacgtcctgt
    15721 gtcaagtgat gaaatcaaca tggtagtcaa tcgtatcgaa cagaaactcc gtggtcgaaa
    15781 tgaaaatgaa attcaaagtg aggacattgg ttcactcgtc atggaggagt tggctgaatt
    15841 ggacgagatt acctatgtac gttttgctag tgtctatcgt agttttaagg atgtcagtga
    15901 gttagagagc ttgctccaac aaatcaccca gtcctctaaa aagaaaaagg aaagataaat
    15961 gaagccaatt gaccgttttt cttatctaaa gaataatcgg gtgtcgcaag atacctcatc
    16021 tctggtacag tgctacctcc cgattatcgg tcaggaggca ctgagccttt atctttatac
    16081 aatcagtttt tgggataatg gtagaaagga atatcttttt tcaagtatcc tcaatcatct
    16141 caactttggg atggatagac tgataaaatc attgaaaatc ttatctgctt ttaatctctt
    16201 gaccctctat caaaaggggg atgtttatca gctagccctc catgctcctc tatctagtca
    16261 agacttcttg gagcatcctg tttatcgcag actcttagag aaaaagattg gcgactctgc
    16321 tgtggaggat ttgaaagttg aaagtgctga tggtgaagaa atacctgtct cactcaatca
    16381 agtctttcca gaattggcag aactaggcag tcaagaagac cttggtctca agaagaaagt
    16441 ggccaatgat tttgacttgg aacattttcg ccagctgatg gctcgagatg gacttcgttt
    16501 tgcggatgag cagtccgatg tcttaaacct ctttgctatt gccgaggaga agaaatggac
    16561 ttggtttgaa acctatcaat tggccaagtc aacagctgtt tctcaggtta tttcaaccaa
    16621 acgcatgcgt gaaaaaattg ctcaaaaacc agtttcctct gactttaatc ctaaggaagc
    16681 aaccattatc aaggaagcta aaagtaagac tgccctgcag ttcttagcag aaatcaagca
    16741 aacacgcaag gggaccatta cccaaacaga aagagaactt ttgcaacaga tggctggctt
    16801 gggcttgctg gacgaagtca tcaatatcat tctcttgttg acctttaata aggtggattc
    16861 ggcaaacatc aatgagaaat atgccatgaa ggtggctaat gactatgcct atcaaaagat
    16921 acattcagca gaagaggccg ttttacgaat tcgtgagcga gggcagaaaa caaaaacaca
    16981 aaaacagaat cagactgccc cagcaaaaac caatattcct aaatggagta acccagatta
    17041 taagaatgaa accagcgagg aaactcgtct ggaactagaa cgtaagaaac aagaactatt
    17101 agctcgatta gaaaaaggag gagattagat ggaaagtgtc ggagacgtac tcaaacgtca
    17161 acccagccgt ttttattatc aagatttggt ccagaaaatc atgaaggacc ctgatgttgc
    17221 ggcttttatc cagcaagaat ccttgactcc agaggaatta aatcgcagta tctccaagtt
    17281 taatcagtac atcaccgagc gtgacaagtt tctccgtggg gatacggatt atattgccaa
    17341 aggctacaag ccgattttgg ttaagaatca tggctatgca gatgtttcat atgaagaaac
    17401 tcctgagcta atcgcggctg aaaaagaagc ggctattaag aatcgtctca agctaatcaa
    17461 tctaccagcc agtctcaaaa aagctagttt ggctcaagtt gatttggatg atttgggacg
    17521 cttgccagtt tttgaaaaac tattagcctt tgtggagcaa tatccagcta ttcgaaaagg
    17581 tctttactta tatggagact ttggtgtggg taaaagtttc atggtggcgg ccttagctca
    17641 tgatttatca gaaaaacgtg gtgtttcatc aactctcctc cactatccta gctttgtcat
    17701 tgatgtcaaa aatgctatca gtgatggcaa tgttaagacc ttggtggatg agattaagct
    17761 gtctgaggtc ctgattttag atgatattgg cgccgagcaa tcaaccgctt gggtgcgtga
    17821 cgaaatcctg caggtcattc tccaatatcg gatgcaggaa aatttaccga cctttttcac
    17881 atccaacttc aactttgaag atttggagaa gcattttgcg aaagtgaaac atggaaatga
    17941 cgaaacctgg gaagccagac gggtcatgga acgcatccgt tatttggctg aggagactcg
    18001 tttagaagga gtaaaccgtc gatgacagaa accattaaat tgatgaaggc tcatacttca
    18061 gtgcgcaggt ttaaagagca agaaattccc caagtagact taaatgagat tttgacagca
    18121 gcccagatgg catcatcttg gaagaatttc caatcctact ctgtgattgt ggtacgaagt
    18181 caagagaaga aagatgcctt gtatgaattg gtacctcaag aagccattcg ccagtctgct
    18241 gttttccttc tctttgtcgg agatttgaac cgagcagaaa agggagcccg acttcatacc
    18301 gacaccttcc aaccccaagg tgtggaaggt ctcttgatta gttcggtcga tgcagctctt
    18361 gctggacaaa acgccttgtt ggcagctgaa agcttgggct atggtggtgt gattatcggt
    18421 ttggttcgat acaagtctga agaagtggca gagctcttta acctacctga ctacacctat
    18481 tctgtctttg ggatggcact gggtgtgcca aatcaacatc atgatatgaa accgagactg
    18541 ccactagaga atgttgtctt tgaggaagaa taccaagaac agtcaactga ggcaatccaa
    18601 gcttatgacc gtgttcaggc tgactatgct ggggcgcgtg cgaccacaag ctggagtcag
    18661 cgcctagcag aacagtttgg tcaagctgaa ccaagctcaa ctagaaaaaa tcttgaacag
    18721 aagaaattat tgtagaaagt gagaaattat ggccctacca actattgcca ttgtaggacg
    18781 tcccaatgtt gggaaatcaa ccctatttaa tcggatcgct ggtgagcgaa tctccattgt
    18841 agaagatgtc gaaggagtga cacgtgaccg tatttatgca acgggtgagt ggctcaatcg
    18901 ttcttttagc atgattgata caggaggaat tgatgatgtc gatgctcctt tcatggaaca
    18961 aatcaagcac caggcagaaa ttgccatgga agaagcagat gttatcgtct ttgtcgtgtc
    19021 cggtaaggaa ggaattaccg atgcagacga atacgtagcc cgtaagcttt ataagaccca
    19081 caaaccagtt atcctcgcag tcaacaaggt ggacaaccct gagatgagaa atgatatcta
    19141 tgatttctat gctctcggtt tgggtgaacc attgcctatc tcatctgtcc atggaatcgg
    19201 tacaggggat gtgctagatg cgatcgtaga aaatcttcca aatgaatatg aggaagaaaa
    19261 tccagatgtc attaagttta gcttgattgg tcgtcctaac gttggaaaat caagcttgat
    19321 caatgctatc ttgggagaag accgtgttat tgctagtcct gttgctggaa caactcgtga
    19381 tgccattgat acccacttta cagatacaga tggtcaagag tttaccatga ttgatacggc
    19441 tggtatgcgt aagtctggta aggtttatga aaatactgag aaatactctg ttatgcgtgc
    19501 catgcgtgct attgaccgtt cagatgtggt cttgatggtc atcaatgcgg aagaaggcat
    19561 tcgtgagtac gacaagcgta tcgcaggatt tgcccatgaa gctggtaaag ggatgattat
    19621 cgtggtcaac aagtgggata cgcttgaaaa agataaccac actatgaaaa actgggaaga
    19681 agatatccgt gagcagttcc aatacctgcc ttacgcaccg attatctttg tatcagcttt
    19741 aaccaagcaa cgtctccaca aacttcctga gatgattaag caaatcagcg aaagtcaaaa
    19801 tacacgtatt ccatcagctg tcttgaacga tgtcatcatg gatgccattg ccatcaaccc
    19861 aacaccgaca gacaaaggaa aacgtctcaa gattttctat gcgacccaag tggcaaccaa
    19921 accaccaacc tttgtcatct ttgtcaatga agaagaactc atgcactttt cttacctgcg
    19981 tttcttggaa aatcaaatcc gcaaggcctt tgtttttgag ggaacaccga ttcatctcat
    20041 cgcaagaaaa cgcaaataaa aaagtagaat ctggaatgac agttccggat ttttttgata
    20101 taataaaata atagaaaacg ctatcaaaag aaggagggtg atgtcggtga tggaacattt
    20161 gtttaaattc ttacttttag caccgtactt ttattttgat aactggattg agaaggccaa
    20221 cagaaatagt aagttttttc caatttttta ttatttttac tggttttaca tccccttcta
    20281 ttctcttttt agccttgctt ggacagttgt ttcagttctg tttttcaata ccgtcttgag
    20341 aaatgtgaca gatatcaagt tatggggcat ttggtttctt tttattctgc tagctattgg
    20401 tatgaattgg ttaacttatt cctgtttcaa agaaatgttt cgcttgagac aggaactagg
    20461 gaagtctaag ggtggaaggc attgatttat atcggtttct atttgtagga ggtggcttat
    20521 ggtacctcta aaatcattta ttacacgcta ttccaaggtt tatattggta ataacattta
    20581 atagaacgaa actaaagaaa acaatttaaa agaggaggtt tgtgatggaa tacttgttta
    20641 aaatgataat cttattaccc tgtttttact ttttcagctg gattgaaaag gcaaacagag
    20701 atagtaagat tttcccaatt ttttactatt tttactggat ttacatcacc ctctatgctc
    20761 tttttagcct tgcttggaca gttttttcag ttctgttttt caatatcgtc ttgagaaatt
    20821 tgacagatat caagttatgg ggtatttggc tattattact acttatcgct tttgctagtg
    20881 actggttaga ctatatctgt ttcaaaaaaa tgcttgactt gagacgggaa ctagggaagt
    20941 ctaaaggcgg aaggcattga tttatactct tcgaaaatct cttcaaacca cgtcaacgtc
    21001 gccttggatt atatatgtaa ctgacttcgt cagtcttatc tacaacctca aagcagtgct
    21061 ttgagcaact tgcggctagt ttcctagttt gctctttgat tttcattgag tattatatta
    21121 ctttctattt gtaggaggtg gcttatgaag attcctctct taactttggc aaggcataaa
    21181 tttgtttatg tcttgcttac tttgcttttt cttgctttgg tttatcgtga tgttttgatg
    21241 acttatttct tttttgatat tcatgcgccc gatctagcta aattcgatgg acaagcaatt
    21301 aaaaatgact tattaaaatc agcattagat tttcgtattc tccagttcaa tctaggtttt
    21361 tatcaatcat ttattattcc aatcatcatt gttttgctag gttttcaata tattgagctg
    21421 aaaaataaag ttttacgatt gagtattgga agagaagtga gctatcaagg gttaaaaaga
    21481 aagttgactt tgcaagttgc aagtatccct tgtttgatat atttagtgac tgtgctgata
    21541 attgcaatta taacctattt ctttgggact ttttctcctc ttgaatggaa ttctctattt
    21601 tctgatggga gtggtttaca aagactccta gatggagaga taaaaagcta tttgttcttt
    21661 acttgtgtcc tactaatcgg tattttcatc aatgcaatct attttttaca aatagttgat
    21721 tatgtgggga atgtgactcg ttcggcaatc acctatttga tgtttctttg gcttggttct
    21781 atgctgctct atagtgcctt gccttactat atggttccta tgacgagttt gatgcaagct
    21841 agctatgggg atgtaagttt gatgaaactc tttactcctt atatccttta tgttgtccct
    21901 tatatggtac ttaaaaaata tgaagataat gtttaagaat tttaacaata tttggctaaa
    21961 gagaaagatc gttttactac ttcgtatagt tctgatgatg attttgataa actatctatt
    22021 gtcaacagcg gttcaaaagc aggatgctgt tatctttttc aagagagaat tgatttcaat
    22081 tttttcctat aatgactatt ctgaagcgaa tttagaaatc cccaaactct tgttaaacct
    22141 ttcgcttttc atggtaggat ggctctctgt cattttactt gaaagtgatt tggcagacca
    22201 ttaccatcac ttgattcgct atcaatcaag ctcctttttc gattatacaa ggaaacgatt
    22261 ggttgtcatt tctaaatttt ttactcaaga tttgtttgtc tggtttcttg gtttacttcc
    22321 gctaggaatt catttcaaaa cagtcgcact tttcttttta cttgctcagt taatgatgtt
    22381 gtacttacta ctgtcttatc tgatagcact gattagtgcg ggcgctggtt tttctttttt
    22441 tctctatttt ttggcatttg tgggacaaga atggatgatg gatcatattg taacagtgta
    22501 tttagtactc ttaagcttat tagttatgtt gattgttagt cgcttggaag agaaatttaa
    22561 gaaaggataa acgatgagac ttgaaattat aaatggacag aaaatttatg ggaaaagacc
    22621 tattttaaat cagttgaatt tggtgtttca atcaggaaaa atttatggac ttaaaggtga
    22681 taatggatct ggcaagacgg ttcttttaaa gatacttgct ggttatatta agcttgacaa
    22741 aggaaaagtt cttcaagatg gtaaagttta cggggtaaaa aatcattata ttcaggatgc
    22801 aggaatttta attgaaaaag tcgagttttt atctcattta tccctgagag aaaatttgga
    22861 actgttaagg tatttttcat ctaaagttac ggaaaaaaga attgcctatt ggattcaata
    22921 ctatgattta caggaatttg aagacgttga ataccgtcat ttatccttgg gaacaaagca
    22981 aaaaatggcc ttgattcaag cctttatttc ctctccttct atactctttc tcgatgaacc
    23041 tatgaatgct ttggatgaga agagtgtgag gttaaccaaa caggtcattt tatcttacct
    23101 gaaaaaagaa aatggtctgg ttatcctgac gtcgcacata tcggaagata tttcagacct
    23161 ttgtacagat gtattagttg tcgaaaatgg acatatacaa tatgtaaagg atatacaatc
    23221 ctaggagatg gcttatggca catctaaaat catttattac acgctattcc aaggtttata
    23281 ttggtttagt tctgctgatc tggctgtctt tcttctttat cccttgggat agagcgattc
    23341 tgggggtaag gattgacatc ttcatcatgc agaaaatctt gctagctttt ggaattctgt
    23401 ccattctcat ggccttgctg tccaagaaag tcagtctctt tgtttttgga ctgatttgct
    23461 gtctttctct ttggattaac ttatttatca catttgccat tttgccgatt tttggcaatt
    23521 aaacagtcat aaaagtcgga gaggttagct tgaaaactaa cctctttttc cttttcaaaa
    23581 tggggattct tccttgaaaa taatcagtaa ttgtgctaaa attaaaggaa cattctaaaa
    23641 tattcggaat ttaaagtaag gaaaaacatg gctaatattt taaaaacaat tatcgaaaat
    23701 gataaaggag aaatccgtcg tctggaaaag atggctgaca aggttttcaa atacgaagac
    23761 caaatggcta ctttgactga cgaccaacta aaagcaaaaa cagttgaatt taaggaacgt
    23821 tatcaaaatg gagaatcact ggattcattg ctttacgaag catttgcggt tgtccgtgaa
    23881 ggtgccaaac gtgtcctagg tctcttccct tataaggttc aggtcatggg ggggattgtt
    23941 cttcaccatg gtgacgtgcc agagatgcgt acaggggaag ggaaaacctt gactgcgacc
    24001 atgccggtat acctcaatgc cctttcaggt aaaggggttc acgtagttac ggttaatgaa
    24061 tacctgtcag aacgtgacgc gactgagatg ggtgaattgt actcttggct tggtttgtca
    24121 gtagggatta acttggctac caaatctcca atggagaaaa aagaagccta tgagtgtgat
    24181 attacttact caactaactc agaaatcgga tttgactacc ttcgtgacaa catggtcgtt
    24241 cgcgccgaaa acatggtaca acgtccgctt aactatgcct tggtcgatga ggttgactct
    24301 atcttgattg acgaggctcg tacacctttg attgtatcag gtgccaatgc ggttgaaacc
    24361 agtcagttgt atcacatggc agaccactat gtaaaatctt tgaacaaaga tgactacatc
    24421 atcgatgtgc agtctaagac tattggtttg tctgattcag ggattgacag ggctgaaagc
    24481 tacttcaaac ttgaaaacct ctatgacatc gaaaacgtgg ctttgactca ctttatcgat
    24541 aacgcccttc gtgccaacta catcatgctt ctcgatattg actatgtggt gagcgaagag
    24601 caagaaatct tgattgtcga ccaatttaca ggtcgtacca tggaaggtcg tcgttattct
    24661 gatggattgc accaagctat tgaagccaaa gaaggtgtgc caatccagga tgaaaccaag
    24721 acatctgcct caatcacgta ccaaaacctt ttccgtatgt acaagaaatt gtctggtatg
    24781 acgggtacag gtaagactga ggaagaagaa ttccgtgaaa tctacaacat tcgtgttatt
    24841 ccaatcccaa caaaccgtcc tgttcaacgt attgaccact cagaccttct ttatgcaagt
    24901 atcgaatcta agtttaaagc ggttgtcgaa gacgttaagg ctcgttacca aaagggtcaa
    24961 cctgtcttgg ttggtacagt agcggttgaa actagtgact acatttctaa gaaattggtt
    25021 gcagctggtg ttcctcacga agtcttgaat gccaaaaacc actatagaga agcccaaatc
    25081 atcatgaatg ctggtcaacg tggtgccgtt accatcgcaa ccaacatggc gggtcgtggt
    25141 accgacatca agcttggtga aggtgttcgt gaacttggag gactttgtgt tattggtaca
    25201 gaacgtcatg aaagtcgtcg tatcgataac cagcttcgtg gacgttcagg tcgtcaagga
    25261 gatccaggtg agtcacaatt ctacctatct cttgaagatg atttgatgaa acgttttggt
    25321 tctgaacgct tgaagggaat ctttgaacgt ttgaacatgt ctgaagaggc gattgagtct
    25381 cgcatgttga cgcgtcaggt tgaagcagct cagaaacgtg tcgaaggaaa taactacgat
    25441 acccgtaaac aagtccttca atacgatgat gtcatgcgtg aacaacgtga gattatctat
    25501 gctcaacgtt acgatgtcat cactgcagat cgtgacttgg cacctgaaat tcaggctatg
    25561 atcaaacgca cgattgaacg tgtcgttgat ggtcatgcgc gtgccaaaca agatgaaaaa
    25621 ctagaggcaa ttttgaactt tgctaagtac aacttgcttc ctgaagattc tattacgatg
    25681 gaagacttgt caggcttgtc tgataaggcc atcaaggaag agcttttcca acgtgccttg
    25741 aaggtttacg atagtcaggt ttcaaaacta cgcgatgaag aagcagttaa agaattccaa
    25801 aaagttttga ttctacgagt ggtggataac aagtggacag atcatatcga tgcccttgat
    25861 caattgcgta acgcggttgg acttcgtggc tatgctcaga acaaccctgt tgttgagtat
    25921 caggcagaag gtttccgtat gtttaatgat atgattggtt cgattgagtt tgatgtgaca
    25981 cgcttgatga tgaaagcaca aattcatgaa caagaaagac cacaggcaga acgtcatatc
    26041 agtacaacag cgactcgcaa tatcgctgct caccaagcaa gtatgccaga agatttggat
    26101 ttgagccaga ttggacgcaa tgaactttgc ccatgtggtt ctggtaagaa atttaaaaac
    26161 tgtcacggta aaagacaata aaatgagata gtttagaggc ggatatcttg tgaaaagtaa
    26221 atttttactg ggtatccgtt tgctttataa ggagatgagt tatggtattt acagcaaaaa
    26281 gctctaaaat aaatatagaa gaagttcgtg ccttgtcaaa attagaaggt caggctttgg
    26341 agaggaaatc acagcgcgat caagagctag aagccattat acgtggagaa gatcagcgaa
    26401 ttctcttggt aatcgggcca tgctcatctg acaacgaaga agctgtcctt gaatacgcta
    26461 agcgtttggc agtcctacaa gaagaagtgg cagatcgtat ctttatggtt atgcgtgttt
    26521 atactgccaa accccgtacc aacggagatg gctataaggg cttgattcac cagcctaacg
    26581 cgacagaagc gcctagtctt atcaatggaa tcaaagccgt tcgccatctt cactatcgtg
    26641 tcatcacaga aacagggatg acaactgctg atgaaatgct ttatcctgaa aaccttccgc
    26701 ttgtagatga tttgatttct tacatggcag ttggtgcccg ttcagttgaa gaccagcaac
    26761 accgctttgt ggcaagtggg gcaggatttt ctactggttt taaaaatcca acctctggaa
    26821 atctcaatgt catgtttaat gggatttatg ctgctcaaaa caaacaaagt ttccttttct
    26881 taggaaaaga agtagaaaca actgggaacc cgctttcaca cgctattctt cgtggtgctc
    26941 ttaatgagta tggaaaaaat attcccaact actattatga caatttaatt gataccattg
    27001 cccaatatga gaaaatgggc ttggaaaatc cttttatcat cattgatacc aatcatgaca
    27061 attctggtaa gcagtatatt gaacagatcc gaattgtccg ccagaccttg attaaccgtg
    27121 cttggaatga aaaaattaag cagttcgttc gtggttttat gattgagtct tatctggaag
    27181 atggtcgaca aaatgagcca gaagtatttg gcaagtctat cacagaccct tgcctgggtt
    27241 gggataacac agaagctctt gtcagagaaa tttacaaaac gttaggagaa taagatggca
    27301 tttattgaaa aaggtcaaga aatcgatatg gaagtcatca aggctgaaac ccaattgtct
    27361 gcggaagcct tgagactcaa ggaaagccgt gacagggaat tggcagatat tatttcaggg
    27421 gaagatgacc gtattctctt ggtgattggt ccttgctctt ctgataatga agaggcggtc
    27481 ttggaatatg ctcgccgttt atccgccttg caaaagaagg tagcggacaa gattttcatg
    27541 gtcatgcgcg tgtatactgc taagcctcgt accaatggag acggctataa aggattggtt
    27601 caccagccag atacttctaa ggctccaagc ctgattaacg gtttgcaggc tgtgcgccag
    27661 ttgcattacc gcgtgattac agagactggt ttgacaacgg cagatgagat gctttatccg
    27721 tcaaatctga tcttggtgga tgacttggtc agctaccatg ccgttggagc tcgttctgtg
    27781 gaagaccaag agcaccgctt tgtggcttct gggattgatg caccagtagg gatgaaaaat
    27841 ccaacctcag gaaatttggg tgttatgttt aacgccatct atgctgctca aaacaagcaa
    27901 accttccttt atcatgggca ggaagttgag acatcaggta atcctttggc ccatgttatc
    27961 ctccgtggag cagtcaacga gtatggcaat tatatgccga attactacta tgaaaaccta
    28021 ctccaagcca ttgaacgcta tgaaaccatg ggacttgaaa atccttttat cctcattgac
    28081 accaaccatg ataactcagg caagcaatat atggagcaga ttcgaattgt tcgccagacc
    28141 ttgcagaatc gtgattggaa tgagaaaatt aaaaagacgg ttcgaggatt tatgattgaa
    28201 tcttacctag cagatggtcg tcaaaaccaa ccagagatct ttggttgctc tattactgac
    28261 ccttgcctag gttgggaaaa tacagaggcc ttggtagaag agatttatgt taccttgaca
    28321 aaataagtga aaaggatgga gttggggaat ctcaactcct tttgatgaga atgatagttg
    28381 gacacggaat tgacatcgaa gaattggctt cgatagaaag cgcagttaca cgacatgaag
    28441 gatttgctaa gcgtgtactg accgctcagg aaatggagcg cttcaccagt ctcaaaggac
    28501 gcaggcaaat agaatattta gctggtcgct ggtcggctaa ggaggccttt tccaaggcta
    28561 tgggaacggg cattagcaag ctcggttttc aggatttgga agtattgaac aatgaacgtg
    28621 gggcgcctta ttttagtcag gcaccatttt caggaaagat ttggctgtct atcagccaca
    28681 ccgatcagtt tgtgacagcc agtgtcattt tggaggaaaa tcatgaaagc tagtccacat
    28741 agaccaacca aggctctgat tcatctggga gctattcgac aaaatattca gcaaatgggg
    28801 gctcatatcc ctcaaggaac gctcaagttg gctgtggtta aggccaatgc ttatggtcat
    28861 ggagctgttg ccgttgccaa ggcaattcaa gatgatgttg atggcttttg cgtttccaat
    28921 atcgatgaag ccattgaact cagacaagct ggactcagca agccaatcct cattttagga
    28981 gtttctgaaa tcgaagctgt tgctctagct aaagaatatg acttcacctt gacagtggct
    29041 ggactggagt ggattcaagc actcttagat aaggaagtgg acctaactgg attgacagtc
    29101 cacctcaaga ttgattcagg gatgggacgg attggtttta gagaggcaag tgaggttgag
    29161 caggctcaag atttgctcca acaacacggt gttcgtgttg aaggaatctt tacccacttt
    29221 gctactgctg atgaggaatc agatgactat tttaatgccc agttagaacg gtttaaaact
    29281 attttagcta gtatgaagga agttccagag ctggttcatg ctagcaattc tgcaacgact
    29341 ctttggcatg tagagactat tttcaatgcg gttcgtatgg gagatgccat gtatggtctc
    29401 aatccaagtg gagcggtctt ggctttgcct tatgatttga taccggcctt gaccttggag
    29461 tctgctctgg ttcatatcaa gacagttcca gctggtgctt gcatgggcta tggagcaact
    29521 tatcaaacgg atagcgagca agtcatcgcg accgtgccaa tcgggtatgc agatggatgg
    29581 acaagagaca tgcaaaattt ctctgtcttg gtagatggcc aagcttgccc aattgtcggc
    29641 agggtttcga tggaccaaat cactattcga ttgcctaagc cttacccact cggaaccaag
    29701 gtaaccttga ttggctccaa tggggataag gaaatcactg caactcaggt agcgacctac
    29761 cgcgtaacca ttaactatga ggtggtttgc ctcctcagcg accgtattcc gagagaatat
    29821 tactagaaaa gaaaggagtg gagcatgaat ctacatcaac ccttgcatgt cttgcctggt
    29881 gtgggaccaa agtcagcaga aaaatacgcc aaactaggaa ttgaaaactt gcaagatctc
    29941 ttgctctact ttcctttccg ttatgaagac ttcaaaatca agcaggtgct ggagctggaa
    30001 gacggtgaga aggcagttct ttctggtcag gtagtgactc ctgctagtgt ccagtattat
    30061 ggtttcaagc gcaatcgcct gcgttttagt ctcaagcagg gagaggtcgt ttttgcggtg
    30121 aatttcttta accagcccta tctggctgat aaaatagagt tgggagcaac ccttgctgtc
    30181 tttggaaaat gggaccgcgc taaggctagt ctgactggga tgaaggttct ggctcaggta
    30241 gaagatgacc tccagcctgt ctatcgtctg gctcagggaa tcagtcaggc cagtctggtt
    30301 aaggtcatca agacggcttt tgatcaggga ctggacctct tgatagaaga aaatctgccc
    30361 cagtctttac tagacaaata caaactcatg tcccgttgtc aggcagtccg agctatgcat
    30421 tttccaaagg atttggcaga atacaagcag gctcttcgcc gtatcaagtt tgaggaactc
    30481 ttttatttcc aaatgcagct gcagatgctc aagtctgaaa atagagttca gggaagtggt
    30541 ctggttctga attggtctca ggaaaaagtg acagcagtta aagtaagtct tccttttgcc
    30601 ctgacccaag ctcaggaaaa gagtttgcag gaaattttaa ctgatatgaa gtccgaccac
    30661 cacatgaatc gtctcctaca aggggatgtg gggagtggaa aaacggtagt cgctggcttg
    30721 gccatgtttg cggcagtgac agcaggttat caggctgccc taatggtacc aacagaaatc
    30781 ctcgcagagc aacactttga gagtttacag aacctttttc ccaatttgaa actggctctc
    30841 ttgacaggtt ccttgaaagc tgcagaaaag agagaggtct tggagaccat tgccaagggt
    30901 gaggctgatt tgattatcgg aactcacgct ctgatacaag atggggtgga gtatgctcgt
    30961 cttggtttga ttattatcga tgagcagcac cgttttggtg tagggcaaag gcgtatttta
    31021 cgggaaaaag gtgacaatcc agatgtcctc atgatgacgg cgactcccat tccacggacg
    31081 cttgccatca cagcctttgg agatatggat gtttccatta tcgaccagat gccagcaggt
    31141 cggaagccta ttgtgacgcg ctggatcaaa catgagcaac tacctcaggt cttgacttgg
    31201 ttagaggggg aaattcaaaa aggttcccaa gcctatgtca tctctccttt gattgaagaa
    31261 tcagaagctc tagatttgaa aaatgccatt gccttatcag aggagttgac gactcatttt
    31321 gcaggcaagg cagaggtggc tcttctacat ggtaggatga agagtgacga aaaagaccag
    31381 atcatgcagg atttcaagga gagaaagacg gatattctgg tttcgacgac ggttattgag
    31441 gttggggtca acgttcccaa tgcgactgtc atgattatca tggatgccga tcgcttcggt
    31501 ctcagtcaac ttcaccagct tagaggtcgt gtcggtcggg gggacaagca gtcctacgct
    31561 gttctcgttg ccaatcccaa gacggattct gggaaagacc gcatgcgtat catgacagaa
    31621 acgaccaatg gatttgtcct tgcggaggaa gatttgaaaa tgcgtggttc tggtgagatt
    31681 tttggaacca gacagtcagg acttccagag ttccaagtgg ctgatattat cgaagatttt
    31741 ccgattttag aagaagcaag aaaggttgct agctacatta gttctataga agcttggcaa
    31801 gaagatccag agtggcgcat gattgccctt catctggaaa agaaagaaca tctggattaa
    31861 gctttctcta aggaaaactt aagctagttt ttaggtttgg ctcttatact aaagtcatca
    31921 aaaagaaacg aggactctca tatgacagta acgattaaag taaattacca aaccactttc
    31981 caaaagaagg aagcaaaaaa ctagtataaa cagaagagag agcgaaatgc tctttttcgt
    32041 ttctaaaact actttcagcc catcatccta aaagtaaaga atctaaattc actttctatt
    32101 tatccttctt tcttgcattg attacataga tatgctacag ttgtggtaac gattacaaaa
    32161 taaaaggagc atgctatgaa aaatccagct ttgctagaag aaattaagac ctatagagga
    32221 agggatgagg ttccggaaga ctttgatgct ttctgggatg gggaagtgaa aaatgtttcc
    32281 acgcttccat cctaccactt ggaggaaaga gatttccaca ttcctcaagt caagtgctat
    32341 gagttaacat ttgaaggaag caaggaagga aaggtctatg cacgcattgt tcttccaaag
    32401 agtgaggaga aggttccact aatcttccat tttcatggtt atatgggacg tggctgggac
    32461 tgggccgaca tgctgggctt caccgtagct ggttacggtg ttgtttccat ggatgtgcgg
    32521 ggccagtcag gttactcaca agacggcttg cgttctcctt taggaaatac cgtgaagggg
    32581 catattatcc gtggtgctat ggaaggtcgg gaccacctct tttataagga tgtttatctg
    32641 gatatttacc agttggtcga aattgttgct agtctgtctc aggttgatga gaagcgtctt
    32701 tctagctatg gtgcctcaca aggaggggct ctagctctag ttgcagcagc gctcaatcct
    32761 cgaattcaga aaacagttgc catttatccc ttcttgtcag acttcagacg ggtgattgag
    32821 attggtaata ctagcgaggc ttacgacgaa cttttccgtt atttcaagtt tcacgacccc
    32881 ttccatgaaa cagaggagga aatcatggcg acccttgcct atatcgatgt caaaaatctt
    32941 gcccatcgta tccaaggtga ggttaagatg attacgggct tggacgacga tgtttgctat
    33001 cccattaccc agtttgcgat ttataatcgt ctgacctgcg ataaaaccta tcgcatcatg
    33061 cctgagtatg ctcacgaagc catgaatgta tttgtcaatg accaagtcta caactggctc
    33121 tgtggaagtg agattccttt taaatatcta aaataaggag tcgactctaa gcacaaaatc
    33181 ttaaaaatta caaacacgca tagtatcagg ggattaaaaa aacttgatac tatgcgtttt
    33241 atcatggaaa tatagtaaaa tgaaacaaga ataggacaaa acgatcagga cagtcaaatc
    33301 gatttctaac aatgttttag aagcaaatgt gtactattct agtttcaatc tattatattt
    33361 atagaatttt ttgttgctag atttgtcaaa ttgcttaaaa taattttttt cagaaagcaa
    33421 aagccgatac ctatcgagta gggtagttct tgctatcgtc aggcttgtct gtaggtgtta
    33481 atacttttca aaaatctctt taaaccacgt cagcttcgcc ttgccgtata tatgttactg
    33541 acttcgtcag ttctatctgc cacctcaaaa cggtgttttg agctgacttt gtcagtctta
    33601 tctacaacct caaaacagtg ttttgagcat catgcggcta gcttcttagt ttgctctttg
    33661 attttcattg agtataaaaa cagatgagtt tctgttttct ttttatggac tataaatgtt
    33721 cagctgaaac tactttcaag gacattatta tataaaagaa ttttttgaaa ctaaaatcta
    33781 ctatattaca ctatattgaa agcgttttaa aaatgaggta taataaattt actaacgctt
    33841 ataaaaagtg atagaatcta tctttatgta tatttaaaga tagattgctg taaaaatagt
    33901 agtagctatg cgaaataaca gatagagaga agggattgaa gcttagaaaa ggggaataat
    33961 atgatattta aggcattcaa gacaaaaaag cagagaaaaa gacaagttga actacttttg
    34021 acagtttttt tcgacagttt tctgattgat ttatttcttc acttatttgg gattgtcccc
    34081 tttaagctgg ataagattct gattgtgagc ttgattatat ttcccattat ttctacaagt
    34141 atttatgctt atgaaaagct atttgaaaaa gtgttcgata aggattgagc aggaagtatg
    34201 gtataaatag cataagctga tgtccatcat ttgcttataa agagatattt tagtttaatt
    34261 gcagcggtgt cctggtagat aaactagatt ggcaggagtc tgattggaga aaggagaggg
    34321 gaaaattggc accaatttga gatagtttgt ttagttcatt tttgtcattt aaatgaactg
    34381 tagtaaaaga aagttaataa aagacaaact aagtgcattt tctggagtaa atgtcttatt
    34441 tcagaaatcg ggatatagat atagagagga tcagtatgaa tcggagtgtt caagaacgta
    34501 agtgtcgtta tagcattagg aaactatcgg taggagcggt ttctatgatt gtaggagcag
    34561 tggtatttgg aacgtctcct gttttagctc aagaaggggc aagtgagcaa cctctggcaa
    34621 atgaaactca actttcgggg gagagctcaa ccctaactga tacagaaaag agccagcctt
    34681 cttcagagac tgaactttct ggcaataagc aagaacaaga aaggaaagat aagcaagaag
    34741 aaaaaattcc aagagattac tatgcacgag atttggaaaa tgtcgaaaca gtgatagaaa
    34801 aagaagatgt tgaaaccaat gcttcaaatg gtcagagagt tgatttatca agtgaactag
    34861 ataaactaaa gaaacttgaa aacgcaacag ttcacatgga gtttaagcca gatgccaagg
    34921 ctccagcatt ctataatctc ttttctgtgt caagtgctac taaaaaagat gagtacttca
    34981 ctatggcagt ttacaataat actgctactc tagaggggcg tggttcggat gggaaacagt
    35041 tttacaataa ttacaacgat gcacccttaa aagttaaacc aggtcagtgg aattctgtga
    35101 ctttcacagt tgaaaaaccg acagcagaat tgccaaatgg tcgagttcgc ctgtatgtta
    35161 atggtgtttt atctcgaaca agtctgaaat ctggcaattt cattaaagat atgccagatg
    35221 taacgcatgt gcaaatcgga gcaaccaagc gtgccaacaa tacggtttgg gggtcaaatc
    35281 tacagattcg gaatctcact gtgtataatc gtgctttaac accagaagag gtacaaaaac
    35341 gtagtcaact ttttaaacgc tcagatttag aaaaaaaact acctgaagga gcggctttaa
    35401 cagagaaaac ggacatattc gaaagcgggc gtaacggtaa accaaataaa gatggaatca
    35461 agagttatcg tattccagca cttctcaaga cagataaagg aactttgatc gcaggtgcag
    35521 atgaacgccg tctccattcg agtgactggg gtgatatcgg tatggtcatc agacgtagtg
    35581 aagataatgg taaaacttgg ggtaaccgag taaccattac caacttacgt gacaatccaa
    35641 aagcttctga cccatcgatc ggttcaccag tgaatatcga tatggtgttg gttcaagatc
    35701 ctgaaaccaa acgaatcttt tctatctatg acatgttccc agaagggaag ggaatctttg
    35761 gaatgtcttc acaaaaagaa gaagcctaca aaaaaatcga tggaaaaacc tatcaaatcc
    35821 tctatcgtga aggagaaaag ggagcttata ccattcgaga aaatggtact gtctatacac
    35881 cagatggtaa ggcgacagac tatcgcgttg ttgtagatcc tgttaaacca gcctatagcg
    35941 acaagggtga tctatacaag ggtaaccaat tactaggcaa tatctacttc acaacaaaca
    36001 aaacttctcc atttagaatt gccaaggata gctatctatg gatgtcctac agtgatgacg
    36061 acgggaagac atggtcagcg cctcaagata ttactccgat ggtcaaagcc gattggatga
    36121 aattcttggg tgtaggtcct ggaacaggaa ttgtacttcg gaatgggcct cacaagggac
    36181 ggattttgat accggtttat acgactaata atgtatctca cttaaatggc tcgcaatctt
    36241 ctcgtgtcat ctattcagat gatcatggaa aaacttggca tgctggagaa gcggtcaacg
    36301 ataaccgtca ggtagacggt caaaagatcc actcttctac gatgaacaat agacgtgcgc
    36361 aaaatacaga atcaacggtg gtacaactaa acaatggaga tgttaaactc tttatgcgtg
    36421 gtttgactgg agatcttcag gttgctacaa gtaaagacgg aggagtgact tgggagaagg
    36481 atatcaaacg ttatccacag gttaaagatg tctatgttca aatgtctgct atccatacga
    36541 tgcataatgg aaaagaatac attatcctca gcaatgcagg tggaccgaat cgtgaaaatg
    36601 ggatggtcca cttggcacgt gtcgaagaaa atggtgagtt gacttggctc aaacacaatc
    36661 caattcaaaa aggagagttt gcctataatt cgctccaaga attaggaaat ggggagtatg
    36721 gcatcttgta tgaacatact gaaaaaggac aaaatgccta taccctatca tttagaaaat
    36781 ttaattggga atttttgagc aaagatctga tttctcctac cgaagcgaaa gtgaagcgaa
    36841 ctagagagat gggcaaagga gagatgggca aaggagttat tggcttggag tttgactcag
    36901 aagtattggt caacaaggct ccaacccttc aattggcaaa tggtaaaaca gcgactttcc
    36961 taacccagta tgatagcaaa accttgttgt ttgcagtaga taaggaagat atcggacagg
    37021 aaattattgg tatagctaaa ggaagcatcg aaagtatgca taatcttcct gtaaatctag
    37081 caggtgccag agttcctggc ggagtaaatg gtagcaaagc agcggtgcat gaagttccag
    37141 aatttacagg gggagttaat ggtacagagc cagctgttca tgaaatcgca gagtataagg
    37201 gatctgattc gcttgtaact cttactacaa aagaagatta tacttacaaa gctcctcttg
    37261 ctcagcaggc acttcctgaa acaggaaaca aggagagtga cctcctagct tcactaggac
    37321 taacagcttt cttccttggt ctgtttacgc tagggaaaaa gagagaacaa taagagaaga
    37381 attctaaaca tttgattttg taaaaataga aggagatagc aggttttcaa gcctgctatc
    37441 tttttttgat gacattcagg ctgatacgaa atcataagag gtctgaaact actttcagag
    37501 tagtctgttc tataaaatat agtagattga aataagatgt gaacaactct atcaggaaag
    37561 tcaaattaat ttatagaaaa attttagcag tcaaggtgta ctgttataga ttcaatatat
    37621 tatgtgacta ttaaccttct cttctcctaa aattgacttt cttgttttct tatcttgtcc
    37681 actcgaaaca agtattgtaa gaatttgatt atttttgaaa gtacttttaa tatacttgat
    37741 atagttaaaa aagatttgaa actaaattcc aaattagaaa aagacttgaa atactaaaaa
    37801 aaaaaaagta tactctaatt gaaaacggta acaaaactaa tttagagaat gaaatataga
    37861 gtatttctct cttaaaagtt tttggtgaaa cgagatgtag aaaggagatt tagccaaaga
    37921 gtctattagt gctagaataa tagattagaa ttattttaga aaaacgaagt gagcagctta
    37981 taaattcaag tccccaaata gattcatact agtatctttt gcaaaaaata aagggcgact
    38041 tccttcatga atatcaattt catctataag gaaggtagct aattgaacta acttatttat
    38101 tctgtttgtc gctagaaaaa tcagacctcc ttgtgaagat tgaggagata ctgaatgaaa
    38161 atcaaagaag aaactagcaa gctagtagca gattgcccaa aacaccgcct tgaggttgta
    38221 gataagactg acctatataa tccaaggtga agcgactgtg gtttgaagag attttcaaag
    38281 agtataggct agagagtagt gtttttatgt ccttctagta gaaaatgcta gacagaagaa
    38341 tggggaactt ggataggaaa aatagattga gaaaggaggt tagaagagat gattattaca
    38401 aaaattagcc atttaggaac ttatgtggga gtaaatccac attttgcaac attaatagat
    38461 tttctagaaa aaacaggact agaaaattta acagaaggtt cgattgctat cgatggtaat
    38521 cgattgtttg ggaattgctt tacttatcta gcagatggtc aagcaggggc tttctttgaa
    38581 acacaccaaa aatatttgga tattcattta gttttggaaa acgaagaagc catggctgtt
    38641 acatcgccgg aaaatgtaag cgttacccaa gaatatgatg aagagaaaga tattgaatta
    38701 tacacaggga aagtggaaca gttggttcag ttgagagctg gcgaatgcct catcactttt
    38761 ccagaagatt tacatcaacc caaggttcgt ataaatgatg aacctgtgaa aaaagttgtc
    38821 tttaaagttg cgatttctta atgtagaaag agaagaacga tgaaaaaaat gagaaagttt
    38881 ttatgtctag ctggaattgc gctagcggct gttgccttgg tagcttgttc aggaaaaaaa
    38941 gaagctacaa ctagtactga accaccaaca gaattatctg gtgagattac aatgtggcac
    39001 tcctttactc aaggaccccg tttagaaagt attcaaaaat cagcagatgc tttcatgcaa
    39061 aagcatccaa aaacgaaaat caagattgaa acattttctt ggaatgactt ctatactaaa
    39121 tggactacag gtttagcaaa tggaaatgtg ccagatatca gtacagctct tcctaaccaa
    39181 gtaatggaaa tggtcaactc agatgctttg gttccgctaa atgattctat caagcgtatt
    39241 ggacaagata aatttaacga aactgcctta aatgaagcaa aaatcggaga tgattactac
    39301 tctgttcctc tttattcaca tgcacaagtc atgtgggtta gaacagattt gttaaaagaa
    39361 cataatattg aggttcctaa aacttgggat caactctatg aagcttctaa aaaattgaaa
    39421 gaagctggag tttatggctt gtctgttccg tttggaacaa atgacttaat ggcaacacgt
    39481 ttcttgaact tctacgtacg tagtggtgga ggaagcctct taacaaaaga tcttaaagca
    39541 gacttgacaa gccaacttgc tcaagatggt attaaatact gggttaaatt gtataaagaa
    39601 atctcacctc aagattcttt gaactttaat gtccttcaac aagctacctt gttctatcaa
    39661 ggaaaaacag catttgactt taactctggc ttccatatcg gaggaattaa tgccaacagt
    39721 cctcaattga ttgattcgat tgatgcttat cctattccaa aaatcaaaga gtctgataaa
    39781 gaccaaggaa ttgaaacctc aaacattcca atggttgttt ggaaaaattc aaaacatcca
    39841 gaagttgcta aagcattctt agaagcactt tataatgaag aagactacgt taaattcctt
    39901 gattcaactc cagtaggtat gttgccaact attaagggga ttagcgattc tgcagcctat
    39961 aaagaaaatg aaactcgtaa gaagtttaaa catgctgaag aagtaattac tgaagctgtt
    40021 aaaaaaggta ctgctattgg ttatgaaaat ggaccaagtg tacaagctgg tatgttgact
    40081 aaccaacaca ttattgaaca aatgttccaa gatatcatta caaatggaac agatcctatg
    40141 aaagcagcaa aagaagcaga aaaacaatta aacgatttat ttgaggctgt tcagtagatg
    40201 taaaagacta gaaaataggt gggctagtga gctgaaaagc tctagcccaa tcttgtaaaa
    40261 gaagggagaa ggagaatggt taaagaacgt aatttaactc gctggatatt tgttttgcca
    40321 gctatgatta tcgtaggatt actctttgtt tatccgtttt tctcgagtat tttttatagc
    40381 tttaccaata agcatttgat tatgcctaat tataaatttg ttggtttggc taactataaa
    40441 gctgtgctat cagatcccaa cttctttaat gcgttcttta attcaattaa gtggaccgtt
    40501 ttctcattag ttggtcaagt tttagtaggg tttgtattgg ctttagctct tcacagagta
    40561 cgccacttca agaaattata taggacctta ttgattgttc cttgggcatt tcctaccatc
    40621 gttattgcct tttcttggca gtggattcta aacggggttt atggctactt acctaatcta
    40681 atcgtaaaat taggtttaat ggaacataca cctgcatttt tgacagatag tacatgggca
    40741 ttcctatgtt tggtgtttat caacatttgg tttggagcac caatgattat ggttaatgtg
    40801 ctttcagctt tgcaaacagt accagaagaa caatttgagg ctgctaagat agatggtgct
    40861 tcaagttggc aggtgttcaa gtttatcgtc tttccacata ttaaagtggt tgtaggactt
    40921 ctagttgttt tgagaactgt atggatcttt aataactttg acattatcta cctcattact
    40981 ggtggtggac cagccaatgc tacaacgacg cttccaattt ttgcttacaa cctgggctgg
    41041 ggaactaaat tgttgggtcg tgcttcagca gttacagtat tgctctttat cttcttggtg
    41101 gcgatttgct ttatctactt tgctatcatc agtaagtggg aaaaggaggg tagaaaataa
    41161 tgaagaagaa atccagtatt tatttagata ttctctcaca tgtactttta gttggtgcga
    41221 ccatcgttgc aattttccca ttggtatgga ttatcatatc ttctgtcaaa gggaaagggg
    41281 aattaactca gtatccaaca cgattttggc ctgaacagtt tacattagat tatttcactc
    41341 atgttatcaa cgatttgcac ttcattgata acattcgaaa cagtttaatc attgccttgg
    41401 ctacaaccct cattgcgatt attatttctg ctatggcagc ctatggtatt gttcgattct
    41461 ttcctaaatt gggagcaatc atgtcgagac tactcgtcat tacctacatt ttcccaccaa
    41521 ttttgttagc aattccctat tcaattgcca ttgctaaagt tgggttaaca aatagtttat
    41581 ttggcttgat gatggtttat ctatctttta gtgttccata tgcagtttgg ctcttagttg
    41641 gatttttcca aacagttcca attggaattg aagaagcggc tagaattgat ggtgcaaata
    41701 aatttgttac gttttataaa gttgtgctac cgattgtagc accaggtatt gtagcaacag
    41761 ctatttatac atttatcaat gcttggaatg aattcctgta tgccttgatt ttgattaaca
    41821 atacaggaaa gatgacagta gcagtagccc ttcgttcact taatggttca gaaatactag
    41881 actggggaga tatgatggca gcgtctgtta ttgtagttct tccatcaatt attttcttct
    41941 ctatcatcca aaataagatt gcaagtggat tatcagaagg atctgtgaag tagacgaaag
    42001 aaggaaaaaa atgaataaaa gaggtcttta ttcaaaacta ggaatttctg ttgtaggcat
    42061 tagtctttta atgggagtcc ccactttgat tcatgcgaat gaattaaact atggtcaact
    42121 gtccatatct cctatttttc aaggaggctc atatcaactg aacaataaga gtatagatat
    42181 cagccctttg ttattagata aattgtctgg agagagtcag acagtagtaa tgaaatttaa
    42241 agcagatgaa ccaaactctc ttcaagcttt gtttggccta tctaatagta aagcaggctt
    42301 taaaaataat tacttttcaa ttttcatgag agattctggt gagataggtg tagaaataag
    42361 agacgcccaa aagggaataa attatttatt ttccagacca gcttcattat ggggaaaaca
    42421 taaaggacag gcagttgaaa atacactagt atttgtatct gattctaaag ataaaacata
    42481 cacaatgtat gttaatggaa tagaagtgtt ctctgaaaca gttgatacat ttttgccaat
    42541 ttcaaatata aatggtatag ataaggcaac actaggagct gttaatcgtg aaggcaagga
    42601 acattacctc gcaaaaagaa gtattgatga aatcagtcta tttaacaaag caattagtga
    42661 tcaggaagtt tcaaatattc ccttgtcaaa tccatttcag ttaattttcc aatcaggaga
    42721 ttctactcaa gctaactatt ttagaatacc gacactatat acattaagta gtggaagagt
    42781 tctatcaagt attgatgcac gttatggtgg gactcatgat tctaaaagta agattaatat
    42841 tgccacttct tatagtgatg ataatgggaa aacgtggagt gagccaattt ttgctatgaa
    42901 gtttaatgac tatgaggagc agttagttta ctggccacga gataataaat taaagaatag
    42961 tcaaattagt ggaagtgctt cattcataga ttcatccatt gttgaagata aaaaatctgg
    43021 gaaaacgata ttactagctg atgttatgcc tgcgggtatt ggaaataata atgcaaataa
    43081 agccgactca ggttttaaag aaataaatgg tcattattat ttaaaactaa agaagaatgg
    43141 agataacgat ttccgttata cagttagaga aaatggtgtc gtttatgatg aaacaactaa
    43201 taaacctaca aattatacta taaatgataa gtatgaagtt ttggagggag gaaagtcttt
    43261 aacagtcgaa caatattcgg ttgattttga tagtggctct ttaagagaaa ggcataatgg
    43321 aaaacaggtt cctatgaatg ttttctacaa agattcgtta tttaaagtga ctcctactaa
    43381 ttatatagca atgacaacta gtcagaatag agaagagagt tgggaacaat ttaagttgtt
    43441 gcctccgttc ttaggagaaa aacataatgg aacttacttg tgtcctggac aaggtttagc
    43501 attaaaatca agtaacagat tgatttttgc aacatatact agtggagaac taacctatct
    43561 catttctgat gatagtggtc aaacatggaa gaaatcctca gcttcaattc cgtttaaaaa
    43621 tgcaacagca gaagcacaaa tggttgaact gagagatggt gtgattagaa cattctttag
    43681 aaccactaca ggtaagatag cttatatgac tagtagagat tctggagaaa catggtcgga
    43741 agtttcgtat attgatggaa tccaacaaac ttcatatggc acacaagtat ctgcaattaa
    43801 atactctcaa ttaattgatg gaaaagaagc agtcattttg agtacaccaa attctagaag
    43861 tggccgtaag ggaggtcaat tagttgtcgg tttagtcaat aaagaagatg atagtattga
    43921 ttggaaatac cactatgata ttgatttgcc ttcgtatggt tatgcctatt ctgcgattac
    43981 agaattgcca aatcatcaca taggtgtact gtttgaaaaa tatgattcgt ggtcgagaaa
    44041 tgaattgcat ttaagcaatg tagttcagta tatagatttg gaaattaatg atttaacaaa
    44101 ataaaggaga aaaacatggt taaatacggt gttgttggag cagggtattt tggagctgaa
    44161 ttggctcgct acatgcaaaa gaatgatgga gcagagatta ctcttctcta tgatccagat
    44221 aatgcagagg cgattgcaga agaattggga gcaaaagtag caagttcctt agatgagttg
    44281 gtttctagcg atgaagtaga ttgtgttatc gtcgcaactc caaataatct tcataaggaa
    44341 ccggttatta aggctgcaca gcatggtaaa aatgttttct gtgaaaaacc aattgcgctt
    44401 tcttatcaag attgtcgcga gatggtagat gcgtgtaaag aaaacaatgt aacctttatg
    44461 gcaggacata ttatgaattt ctttaatggt gttcatcatg caaaagaact cattaatcaa
    44521 ggagttatcg gagacgttct atattgtcat acagctcgta atggttggga agaacaacaa
    44581 ccgtcagtgt catggaaaaa aattcgtgaa aaatcaggtg gtcacttgta tcaccacatc
    44641 catgaattgg attgcgttca attccttatg gggggcatgc ctgaaactgt aaccatgaca
    44701 ggtggaaatg tggcccatga aggtgaacat ttcggtgatg aagatgatat gatttttgtc
    44761 aatatggaat tttctaataa gcgttttgcc ttgttagaat ggggttcagc ttatcgttgg
    44821 ggtgaacatt atgtcttaat ccaaggaagc aaaggtgcca tccgcttaga cttattcaac
    44881 tgtaaaggaa ctcttaagct agatgggcaa gaaagctatt tcttgattca cgaatcgcaa
    44941 gaagaagatg atgatcggac tcgtatctat catagtacag agatggatgg agcaattgct
    45001 tatggtaaac caggtaaacg tactccatta tggctatcat ctgtcattga taaagaaatg
    45061 cgctatctgc atgagattat ggaaggagct ccagtatcag aagaatttgc aaaacttttg
    45121 acaggtgaag ctgccctaga agcaattgct actgcagatg cttgtaccca gtctatgttt
    45181 gaagatcgca aagtaaaatt gtcagaaatt gtaaaataaa ttttggtatt ctcctattta
    45241 taggtcgact tgctcctctg aaagtacttt tagaggagct gtttgacttt gctagttttt
    45301 gaaactgaaa tctattatac tacaaactat tgaaagcgtt ttaattttaa ggtataataa
    45361 tctcatagaa ataaagaaaa ggaggaaaga ggatgccaca gattagcaaa gaagccttga
    45421 ttgagcaaat caaagatgga atcatcgttt cttgtcaggc tcttcctcat gaaccgcttt
    45481 atacagaagc gggaggggtg attcccttgc tggtcaaagc ggctgagcaa ggtggagcag
    45541 tcggtatccg agcaaacagt gttcgcgata tcaaggaaat taaggaagtc actaaacttc
    45601 caatcattgg gattatcaaa cgtgattatc cacctcagga acccttcatc acggctacta
    45661 tgaaagaagt tgatgaattg gcagaactgg acatcgaggt gattgctctg gattgtacca
    45721 agcgtgaacg ctacgatggt ttggaaattc aagagttcat tcgtcaggtt aaggagaaat
    45781 atcctaatca gcttttgatg gctgatacta gtatcttcga agaagggcta gcagctgtag
    45841 aagcaggaat tgactttgtc ggaacaacct tatcaggcta cacatcctac agtccaaaag
    45901 tagacggtcc agattttgaa ttgattaaga aactctgtga tgccggtgta gatgtcattg
    45961 cagaaggaaa aattcataca ccagaacaag ccaaacaaat ccttgaatat ggagtgcgag
    46021 gcatcgttgt tggtggcgcc attactagac caaaagagat tacagaacgc ttcgttgcta
    46081 gtcttaaata agatgtgagg gggagtttta tgtttaaagt tttacaaaaa gttggaaaag
    46141 cttttatgtt acctatagct atacttcctg cagcaggtct acttttgggg attggtggtg
    46201 cactttcaaa cccaaccacg atagcaactt atccaatact agacaatagt atttttcaat
    46261 caatattcca agtaatgagc tctgcaggag aggttgtatt cagtaatttg tcactacttc
    46321 tctgtgtggg attatgtatt ggcttagcga aacgagataa aggaaccgct gcgttagcag
    46381 gagtaactgg ttacttagtt atgactgcaa cgatcaaagc tttagtaaaa ctttttatgg
    46441 cagaaggatc tgcaattgat actggagtta ttggagcatt agttgtcgga atagttgccg
    46501 tatatttgca caaccgatat aacaatattc aattaccttc cgctttagga ttctttggag
    46561 gttcacgctt cgttcctatt gttacatcgt tctcttctat cttgattggc tttgtcttct
    46621 ttgttatttg gccacctttc caacaacttc ttgtttctac aggtggatat atttctcagg
    46681 cgggtccaat tggaactttt ctatatggat ttttaatgag actttctgga gcagtaggct
    46741 tacatcatat aatttaccct atgttttggt atactgaact tggtggtgtt gaaactgttg
    46801 caggacaaac agtggttgga gctcaaaaaa tattttttgc tcaattagcc gatttggccc
    46861 attctggatt atttacagaa ggaacaaggt tttttgcagg tcgtttctca acaatgatgt
    46921 tcggtttacc ggctgcctgt ttagcgatgt accatagtgt tcctaaaaat cgtcgtaaaa
    46981 aatacgcggg tttgtttttg ggagttgctt taacatcttt tattaccggt attacagaac
    47041 caattgaatt tatgtttcta ttcgttagtc cggttctata tgttgttcac gcattccttg
    47101 atggtgttag cttctttatt gcagacgtct taaatatttc aataggaaac acattttcag
    47161 gaggtgtaat cgatttcact ttatttggaa ttttgcaggg gaacgctaag acgaattggg
    47221 ttcttcagat tccatttgga cttatttgga gtgttttgta ttatattatt tttagatggt
    47281 tcattactca attcaacgtt ctaacgccag ggcgaggaga agaagtagat tctaaagaaa
    47341 tttctgaatc cgcagattca acttcaaata ctgcagatta tttaaaacag gatagcctac
    47401 aaattatcag agccttgggt ggatcaaata atatagaaga tgtagatgct tgtgtgacac
    47461 gtttacgtgt agctgtaaaa gaagttaatc aagttgataa agcactttta aaacaaattg
    47521 gtgcagttga tgtcttagaa gtgaagggtg gcattcaagc aatatatgga gcaaaagcaa
    47581 tcttatataa aaatagtatt aatgaaattt taggtgtaga tgattaagta cttactgact
    47641 taataaaaaa cagaggagag tgatggatga gtaggatgaa atgaaatcgc atacaagaaa
    47701 taaagaactc attatccaag ttggatacgc ttattacata ggagaataca aatgaaattt
    47761 agaaaattag cttgtacagt acttgcgggt gctgcggttc ttggtcttgc tgcttgtggc
    47821 aattctggcg gaagtaaaga tgctgccaaa tcaggtggtg acggtgccaa aacagaaatc
    47881 acttggtggg cattcccagt atttacccaa gaaaaaactg gtgacggtgt tggaacttat
    47941 gaaaaatcaa tcatcgaagc gtttgaaaaa gcaaacccag atataaaagt gaaattggaa
    48001 accatcgact tcaagtcagg tcctgaaaaa atcacaacag ccatcgaagc aggaacagct
    48061 ccagacgtac tctttgatgc accaggacgt atcatccaat acggtaaaaa cggtaaattg
    48121 gctgagttga acgacctctt cacagatgaa tttgttaaag atgtcaacaa tgaaaacatc
    48181 gtacaagcaa gtaaagctgg agacaaggct tatatgtatc cgattagttc tgccccattc
    48241 tacatggcaa tgaacaagaa aatgttagaa gatgctggag tagcaaacct tgtaaaagaa
    48301 ggttggacaa ctgatgattt tgaaaaagta ttgaaagcac ttaaagacaa gggttacaca
    48361 ccaggttcat tgttcagttc tggtcaaggg ggagaccaag gaacacgtgc ctttatctct
    48421 aacctttata gcggttctgt aacagatgaa aaagttagca aatatacaac tgatgatcct
    48481 aaattcgtca aaggtcttga aaaagcaact agctggatta aagacaattt gatcaataat
    48541 ggttcacaat ttgacggtgg ggcagatatc caaaactttg ccaacggtca aacatcttac
    48601 acaatccttt gggcaccagc tcaaaatggt atccaagcta aacttttaga agcaagtaag
    48661 gtagaagtgg tagaagtacc attcccatca gacgaaggta agccagctct tgaatacctt
    48721 gtaaacggat ttgcagtatt caacaataaa gacgacaaga aagtcgctgc atctaagaaa
    48781 ttcatccagt ttatcgcaga tgacaaagag tggggaccta aagacgtagt tcgtacaggt
    48841 gctttcccag tccgtacttc atttggaaaa ctttatgaag acaaacgcat ggaaacaatc
    48901 agcggctgga ctcaatacta ctcaccatac tacaacacta ttgatggttt tgctgaaatg
    48961 agaacacttt ggttcccaat gttgcaatct gtatcaaatg gtgacgaaaa accagcggat
    49021 gctttgaaag ccttcactga aaaagcaaac gaaacaatca aaaaagctat gaaacaatag
    49081 tccttagtta ttctataaaa agtagttttt taaagaacct aagagtgtat accccctttt
    49141 ccctctacac agatagtgta agaaaagggg gcttttgttt aaaatgtaag aaactgtcac
    49201 gaaattaaaa tgaagttctt acataagcga atcataaaaa atttcatttt gattttaaaa
    49261 cagttcaaga aagtcaaaaa attattctat ttgaaagaga ggtgccgact gtgaaagtca
    49321 ataaaatccg tatgcgggaa acagtgattt cctacgcttt cctagcacca gtattattct
    49381 tctttgtcat ctttgtgttg gctccgatgg tgatgggctt cattacaagt ttctttaact
    49441 actcaatgac taaatttgag tttgtaggct tggataacta tatccgtatg tttaaagatc
    49501 ctgtctttac aaaatctctg attaacacag ttattttggt tattggatct gtaccagttg
    49561 ttgttctatt ctcactcttt gtagcatctc agacctatca tcaaaatgtc attgccagat
    49621 ccttctaccg tttcgtcttc ttccttcctg ttgtaacggg tagtgttgcc gtgacagttg
    49681 tttggaaatg gatttatgac ccactatcag ggattctaaa ctttgtcctt aagtccagcc
    49741 acatcatcag ccaaaacatt tcttggttgg gagataaaaa ctgggcattg atggcgatta
    49801 tgattattct cttgaccact tcagttggtc agcccatcat cctttatatc gctgccatgg
    49861 ggaatattga caattcactg gttgaagcgg cgcgtgttga tggtgcaact gagtttcaag
    49921 ttttttggaa gattaaatgg ccaagccttc ttccaacaac tctttatatt gcaatcatca
    49981 caacaattaa ctcattccag tgtttcgcct tgattcagct tttgacatct ggtggtccaa
    50041 actactcaac aagtaccttg atgtactacc tttacgaaaa agccttccaa ttgacagaat
    50101 acggctatgc caacacaatt ggtgtcttct tggcagtcat gattgctatc gtaagctttg
    50161 ttcaatttaa agtacttgga aacgacgtag aatactaaag aaaggagaca gctatgcaat
    50221 ctacagaaaa aaaaccatta acagccttta ctgttatttc aacaatcatt ttgctcttgt
    50281 tgactgtgct gttcatcttt ccattctact ggattttgac aggggcattc aaatcacaac
    50341 ctgatacaat tgttattcct cctcagtggt tccctaaaat gccaaccatg gaaaacttcc
    50401 aacaactcat ggtgcagaac cctgccttgc aatggatgtg gaactcagta tttatctcat
    50461 tggtaaccat gttcttagtt tgtgcaacct catctctagc aggttatgta ttggctaaaa
    50521 aacgtttcta tggtcaacgc attctatttg ctatctttat cgctgctatg gcgcttccaa
    50581 aacaagttgt ccttgtacca ttggtacgta tcgtcaactt catgggaatc cacgatactc
    50641 tctgggcagt tatcttgcct ttgattggat ggccattcgg tgtcttcctc atgaaacaat
    50701 ttagtgaaaa tattcctaca gagttgcttg aatcagctaa aatcgacggt tgtggtgaga
    50761 ttcgtacctt ctggagtgta gccttcccga ttgtgaaacc agggtttgca gcccttgcaa
    50821 tctttacctt catcaatact tggaatgact acttcatgca gttggtaatg ttgacttcac
    50881 gtaacaattt gaccatctca cttggggttg cgaccatgca ggctgaaatg gcaaccaact
    50941 atggtttgat tatggcagga gctgcccttg ctgctgttcc aatcgtcaca gtcttccttg
    51001 tcttccaaaa atccttcaca cagggtatta ctatgggagc ggtcaaagga taatactctg
    51061 cgaaaatcga atgcaaacta cgtcagcttc accttgccat acttaagtat tgcctgcggt
    51121 tagcttccta gtttgttctt caattttcat tgagtatagg aaaatcaatc tatcaagata
    51181 cagaagtata ttttatagat ttagagaata tagaagttat aagtgtctac aaaatggagg
    51241 gtatgcagtt actttatgaa gttttgtcag acacttatca acttaagaat gattttagtt
    51301 aattatcaga aacgaaggaa agagtatgat ttttgacgat ttgaaaaaca tcacctttta
    51361 caaagggatt catcccaatt tagacaaagc tatcgactac ctctaccaac atcgtaaaga
    51421 ttcattcgaa ttaggaaagt atgagattga tggagataag gtctttctag ttgttcagga
    51481 aaatgtcctc aatcaagttg agaataatca atttgaacac cataagaact atgcagattt
    51541 gcatttgctg atagaagggc atgaatattc gagctacggt tcacgtatca aagacgaggc
    51601 agtagcattc gacgaagcga ctgacattgg ctttgttcat tgtcatgaac actacccact
    51661 cttgttgggt tatcataatt ttgcgatttt cttcccaggt gagccacatc agccaactgg
    51721 ctatgcaggt atggaagaaa aggttcgaaa atatctcttt aaaattttga ttgattaaaa
    51781 ataggatgaa ttgttttttt gtaaagcttt gacaatactc taccatgaaa ttgatcttta
    51841 tgaggtagag aaatgagaat aaaatattta aaaattggta tcttctaagc atgctgcaag
    51901 agctagtttc ttagatggac aggaaattac agttgatgag atgtcctgga taattaatgg
    51961 cattgtgaat gcattgattg gtagatacat aaaattaggt acttatgcag ctaagtatgg
    52021 tattggtata gcacgctcga tcttaagtag ggcagctgca actgcagcag caagagtagg
    52081 attactgacc aagatttctg gatggatttt acgagtagct gtgaatgtag ctgatgtata
    52141 tggtaatttc gccaacaata ttgctgcagc ttgggatgca tatgataaaa ttcctaacaa
    52201 tggtcgtata aacttttaaa atacgagaat gaaagcactt tgtatttttt tattgaatat
    52261 gttagcttgg acagtgcttg caatgataat tcgtggaggg ctagatggat ttgataggca
    52321 tacttggagt actattttaa ttgcttcgct gttcggggta tatgattata agcccataga
    52381 taaaaataga aaaaagtcca aaagaaaaaa gaaaaaatag atttgttcat ggtagggact
    52441 tatgaaagct ttactgacaa aaaagaaaac agtttacaaa gaaaaatgat ggaggagcaa
    52501 acatggcaca aaaaggagta agccttatca aggcagcatt tgatacagat aactttctca
    52561 tgcgttttag tgagaaggtc ttggacatcg tgacagccaa tcttcttttt gtcgtctctt
    52621 gtttacccat cgtgacgatt ggagtggcta aaatcagcct ctacgagacc atgttcgaag
    52681 ttaagaagag cagacgggtg cctgttttta aaatctatct aagatctttc aagcaaaatc
    52741 tgaaactagg tcttcagctg ggtttaatgg agttaggaat tgtgtttctt accctttcag
    52801 atctctatct tttctggggt caaacagctc tgcccttcca attgctgaaa gccatttgtt
    52861 taggtattct gatttttctt actatcgtga tgctggctag ttaccctatc gcggcacgtt
    52921 atgacctatc ttggaaagaa attcttcaaa aaggattgat gttggctagt tttaactttc
    52981 cttggttctt cctcatgtta gccattcttg tcctcattgt gatggttctt tatctgtccg
    53041 ccttcagtct actcttaggt ggctcagtct tcctactttt tgggtttgga ctattggtct
    53101 ttatccagac tggattgatg gagaaaattt tcgcaaaata ccaataggag ctttatttct
    53161 gaaactactt tcaaaggctc caaacgctat tctataagcg agaaactaaa atctaagtgt
    53221 ctataagata ttgaaagcct tttcggtaaa gtgtatacta aagaagtaaa gaaaaatctg
    53281 ctttagcaga aaataaaaat aataggagaa aatctatgtc agatttgaaa aaatacgaag
    53341 gtgtcattcc agccttctac gcatgttatg atgagcaagg agaagtaagt ccagagcgta
    53401 cgcgtgcctt ggttcaatac ttcattgata aaggtgttca aggtctttat gtcaatggtt
    53461 cttctggtga atgtatctac caaagcgttg aagatcgcaa gttgattttg gaagaagtca
    53521 tggcggtagc taaaggtaaa ttgaccatta ttgcccatgt tgcctgcaac aatactaaag
    53581 acagtatgga acttgctcgc catgctgaaa gcttgggagt agatgctatt gcaacgattc
    53641 caccaattta tttccgcttg ccagaatact cagttgccaa atactggaac gatatcagtt
    53701 ctgcagctcc aaacacagac tacgtgattt acaacattcc tcaattggca ggggttgctt
    53761 tgactccaag cctttacaca gaaatgttga aaaatcctcg tgttatcggt gtgaagaact
    53821 cttctatgcc agttcaagat atccaaacct ttgtcagcct tggtggggaa gaccatatcg
    53881 tctttaacgg tccagatgaa cagttcctag gtggtcgcct catgggtgcc aaagctggta
    53941 tcggtggtac ttatggtgct atgccagaac tcttcttgaa actcaaccaa ttgattgcgg
    54001 ataaagattt agaaacagcg cgtgagttac agtatgctat caacgctatc attggtaaat
    54061 tgactgctac acatggaaat atgtacggtg tcatcaaaga agttttgaag atcaatgaag
    54121 gcttgaatat tggttctgtt cgttcaccat tgacaccagt gactgaagaa gatcgtccag
    54181 ttgtagaagc ggctgctgcc ttgattcgtg aaaccaagga gagcttcctc taatctaaaa
    54241 ggaggtattt atgacatact acgttgcaat tgatatcggt ggaaccaaca tcaagtatgg
    54301 tttggttgat caagaggggc aacttcttga atcgcatgaa atgccaactg aggcgcataa
    54361 gggtggacct catatcttac aaaagaccaa agatatcgta gctagttatt tagaaaaagg
    54421 cccagtagca ggtgttgcca tatcttctgc tgggatggtg gatccggata agggtgagat
    54481 tttctatgct gggccgcaaa tccctaacta cgcaggcacc cagttcaaaa aggaaatcga
    54541 agaaagcttt actattcctt gtgagattga aaatgatgtc aactgtgcag gtcttgctga
    54601 ggcagtatct ggttcaggca agggagcaag tgtgacactt tgcttgacca ttggaaccgg
    54661 tatcggtggt tgcttgatta tggataggaa agtcttccat ggttttagca attcagcctg
    54721 tgaagtcggg tatatgcata tgcaggatgg agcttttcaa gacttggctt ctacaacagc
    54781 tttagtgaaa tatgtagctg aagcccatgg agaagatgtt gatcagtgga atggccgtag
    54841 aattttcaaa gaagccactg aaggaaacaa aatctgcatg gaaggtattg accgtatggt
    54901 tgactatcta ggaaaaggtc tggcaaatat ttgctacgtt gccaatccag aagtggttat
    54961 tcttggtggt ggtatcatgg ggcaagaggc tatcctcaaa cctaagatcc gcacagcctt
    55021 gaaagaggct ttggtaccaa gtttagcaga aaaaacacga ttagaatttg cccatcacca
    55081 aaatacagca gggatgttgg gtgcatatta tcattttaag acaaaacaat cctagtttgg
    55141 ctcagccaaa ctaggatttt cttacacgtt tttgtctacg atagccgttg agttttttat
    55201 tttcccagta gctattaaag attttttcct tgctttcgcg attgatttcc aaaaagtagg
    55261 cataaatcaa atcgataaag aagagcatag gaagttgagc ggatattcgt tggatatagg
    55321 agggttggct gtgggtggct acaagaacag tctctgtata ggtctggcta tctttattgg
    55381 gaacacttgt aaagagtaca gtctttgccc ccatctcctt ggcatctaat agactatcta
    55441 aaatagaagg agttgagcct gaaagtgaga agcccagtac tagacaattt tcatccatga
    55501 tgctggttgt ccaggcaaag ccgtcttggt ctgtcaaagc ttcgcagacc acacctagtc
    55561 gcataaaacg taatttcatt tcacgggcga cgaggccaga actccctgtt ccaaagaagt
    55621 agatacgctc agcatcttcg attagctggg caattcgctc tagttggatt tcgtcaatca
    55681 agtcttgtgt ttgttccctc atattgctat aacttctgag gactcgtttg gtcagtggac
    55741 tgtgcttgga gactcggttg gcttgatttt ctgcctgatg ttggtattgg aaaataaatt
    55801 ctcggtagcc agtaaagcca cactttttag caaagcgggt caaagcagct tgagaaatat
    55861 gtaatttttg ggtgacttgt tgagaagata aatcatctgt aatcgtttca gcttgcaaaa
    55921 aatagcgagc gatttcttgc tctaggtctg tcatttcttc aaaatgtgaa tcaatgatag
    55981 ttgcgatatc tggtttgtcc atagggaaag ctcctttaca tgagtcatac tggaagacta
    56041 gatcagggaa tagtcacact tcattataac acataatata aggatagata aataaaaacg
    56101 catctctgtt ttaaaaacga aaaaatcgaa aaagcttctc tcttttccat aattttctac
    56161 tcaaattgtg gtacaattaa gagtaagatt ttaagttaga aatgagactg atttgtatga
    56221 gaaaatttaa cagccattcg attccgattc ggcttaattt attgttttca atcgtcattt
    56281 tactctttat gaccattatt ggtcgtttgt tgtatatgca ggttttgaac aaggattttt
    56341 acgaaaaaaa gctagcctca gctagtcaga ccaagattac aagcagttca gcccgtgggg
    56401 aaatttatga tgctagtgga aaacctttgg tagaaaatac gttaaagcag gttgtttcct
    56461 ttacgcgtag caataaaatg acggctacag acttaaaaga aacagctaaa aagttactga
    56521 cttatgtgag catcagttct ccaaatttga caaaacgcca gctggcggat tactatttgg
    56581 ctgatcctga aatctataaa aaaacagtgg aagctctccc aagcgagaaa cgcttggatt
    56641 cagatggcaa tcgtctatcc gaatcagaac tgtataacaa tgcggtcgat agtgtccaaa
    56701 caagtcaact aaactataca gaggatgaaa agaaagaaat ctatcttttt agtcagttaa
    56761 atgctgttgg aaactttgcg acaggaacca ttgctacaga tcctctaaat gattctcagg
    56821 tggctgttat tgcctctatt tcaaaggaga tgcctggcat tagtatttct acttcttggg
    56881 atagaaaggt tttggaaact tccctttctt ctatagttgg gagtgtatcc agtgaaaaag
    56941 ctggtctccc agcggaagaa gcagaagcct atcttaaaaa aggctattct ctaaatgacc
    57001 gtgtaggaac ctcctatttg gaaaagcaat atgaagagac cttacaagga aaacgatcgg
    57061 taaaagaaat ccatctggat aaatatggca atatggaaag cgtggataca attgaggaag
    57121 gtagtaaggg aaacaatatc aaactgacca ttgatttggc cttccaagat agcgtggatg
    57181 ctttgctgaa aagttatttc aattctgagc tagaaaatgg tggagccaag tattctgaag
    57241 gtgtctatgc agtcgccctt aacccaaaaa caggtgcggt tttgtctatg tcagggatta
    57301 aacatgactt gaaaacggga gagttgacgc ctgattcctt gggaacggta accaatgtct
    57361 ttgttccagg ttcggttgtc aaggcggcga ccatcagctc aggttgggaa aatggagtct
    57421 tgtcaggaaa ccagaccttg acagaccagt ccattgtctt ccaaggttca gctcccatca
    57481 attcttggta tactcaggct tacggttcat tccctatcac agcggtccaa gctctggagt
    57541 attcatcaaa tacctatatg gtccaaacag ccttaggtct tatggggcaa acctatcaac
    57601 ccaatatgtt tgtcggcacc agcaatctag agtctgctat ggagaaactg cgttcaacct
    57661 ttggcgaata tggcttgggt actgcgacag gaattgacct accagatgaa tctactggat
    57721 ttgttcccaa agagtatagc tttgctaatt acattactaa tgcctttggg cagtttgata
    57781 actatacgcc gatgcagttg gctcagtatg tagcaactat tgcaaataat ggtgttcgtg
    57841 tggctcctcg tattgttgaa ggcatttatg gtaataatga taagggagga ctgggtgact
    57901 tgattcagca actgcaaccg acagagataa ataaggtcaa tatatccgac tccgatatga
    57961 gcatcttgca ccaaggtttt tatcaggttg cccatggtac tagtggattg acaactggac
    58021 gtgccttttc aaatggtgcc ttggtatcca ttagcggaaa aacaggtaca gccgaaagct
    58081 atgtggcaga tggtcagcaa gcaaccaata ccaatgcggt ggcctatgcc ccatctgata
    58141 atccccaaat cgctgtcgca gtggtctttc ctcataatac caatctaaca aatggtgtag
    58201 gaccttccat tgcgcgtgac attatcaatc tgtatcaaaa ataccatcca atgaactaga
    58261 aaggaaatta tgctttatcc aacacctatt gccaagttga ttgacagtta ttctaagtta
    58321 ccaggtatcg ggattaagac ggctacgcgt ctggcctttt atacgattgg gatgtctgct
    58381 gatgatgtca atgaatttgc aaaaaatctc ctttctgcta agagagaatt gacatattgt
    58441 tctatttgtg gacgtttgac agacgacgat ccttgttcta tctgtactga tccgactcgt
    58501 gaccagacaa caattttagt tcttgaggat agtagagatg tggcagccat ggaaaatatc
    58561 caagaatacc atggactcta tcatgtcctg catggcctca tttctcctat gaatggtatc
    58621 agtccggacg atatcaatct caagagcctt atgactcgtc ttatggatag tgaggtttca
    58681 gaagtgattg tggcgactaa tgctacagcg gatggtgaag cgacttccat gtatctttca
    58741 cgtttgctca agccagctgg tatcaaggtt acgcgtctag cacgaggtct cgctgtggga
    58801 gcggacattg agtatgcgga cgaagtgaca ctcttacgag cgattgaaaa tcggacagag
    58861 ttgtaagtgt aggcaaattt acgaactccc ttcatttata aaaaatcaaa gaggctgaaa
    58921 atcgttccta tcggcctctt tttgtatagt gtgatgagta ggatcgggtt caagttttaa
    58981 aaaaccaagc aaatatgata tactaaagag cgagtattct agtagaatta ggacaaataa
    59041 tatgaaacaa acgattattc ttttatatgg tggacggagt gcggaacgcg aagtctctgt
    59101 cctttcagct gagagtgtca tgcgtgcggt caattacgac cgtttcacag tcaagacttt
    59161 ctttatcagt cagtcaggtg actttatcaa aacacaggaa tttagtcatg ctccggggca
    59221 agaagaccgt ctcatgacca atgaaaccat tgattgggat aagaaagttg caccaagtgc
    59281 tatctacgaa gaaggtgcag tggtctttcc agtccttcac gggccaatgg gagaagatgg
    59341 ctctgttcaa ggattcttgg aagttttgaa aatgccttac gttggttgca acattttgtc
    59401 atcaagtctt gccatggata aaatcacgac taagcgtgtt ctggaatctg ctggtattgc
    59461 ccaagttcct tatgtggcta tcgttgaagg cgatgatgtg actgctaaaa tcgctgaagt
    59521 ggaagaaaaa ttggcttatc cagtcttcac taagccgtca aacatggggt ctagtgtcgg
    59581 tatttctaag tctgaaaacc aagaagaact ccgtcaagcc ttaaaacttg ccttccgata
    59641 tgacagccgt gtcttggttg agcaaggagt gaatgcccgt gaaattgagg ttggcctctt
    59701 gggtaactac gatgtcaaga gcacgctacc tggagaagtt gtcaaggacg ttgcctttta
    59761 tgactacgat gccaagtata ttgataacaa gattactatg gatattcctg ccaaaatcag
    59821 tgatgatgtg gtagctgtca tgcgtcaaaa tgcagaaaca gccttccgtg ccattggtgg
    59881 ccttggtcta tctcgttgcg atttcttcta tacagataag ggagagattt ttctcaacga
    59941 gctcaatacc atgccaggtt tcacccagtg gtctatgtac ccactacttt gggacaatat
    60001 ggggatcagc tacccagaac taatcgagcg tttggttgac cttgccaagg aaagttttga
    60061 caagcgcgaa gcgcatttga tataaaatga aagagagggt agaagccaga accatcactg
    60121 caaggtgact agagttctcg gacttcagcc ctttttaaag gagtagaaat gaaattaaca
    60181 atccatgaag ttgcccaagt tgttggagcc aaaaatgata tcagtatctt tgaggacacc
    60241 cagttagaaa aagctgagtt tgatagtcgt ttgattggaa ctggagattt atttgtgccc
    60301 ctaaaaggtg tgcgtgacgg tcatgacttt atcgaaagag cttttgaaaa tggtgcggca
    60361 gcaaccctgt ctgagaaaga ggttgcaaat catccctaca ttctagtaga tgatgttttg
    60421 actgcctttc aacaactagc tgcctactat cttgaaaaaa cagctgttga tgtctttgcg
    60481 gttacgggtt caaatggcaa gacaacgact aaggatatgt tggcgcattt actgtcaaca
    60541 agatacaaga cctacaaaac acaaggcaat tacaataatg agattggcct tccttacaca
    60601 gttctccaca tgcctgaagg aacagaaaag ttggtcttgg agatggggca ggaccacttg
    60661 ggagatattc atctcttgtc tgaattggct cgtccaaaaa cagccatcgt gaccttggtt
    60721 ggagaagctc atttggcctt tttcaaagat cgttctgaga ttgctaaagg aaaaatgcaa
    60781 attgcagacg gaatggcttc aggttccttg cttttagcgc cggctgaccc tatcgtagag
    60841 gactatttgc caactgataa aaaggtggtt cgttttggtc aaggagcaga gctggaaatt
    60901 actgacttgg ttgagcgcaa agatagtctg accttcaagg ccaatttctt agagcaagtc
    60961 cttgatttgc cagtaactgg caagtacaat gcgacaaatg ctatgattgc atcctatgtt
    61021 gccttgcaag aaggagtttc agaggagcaa attcgtttgg ccttccaaca tcttgaattg
    61081 acgcgtaacc gtaccgagtg gaagaaagca gccaatggag cagacatctt gtcagatgtt
    61141 tacaatgcca atccaactgc tatgaagctg attttagaga ccttctctgc cattccagcc
    61201 aatgaaggtg gcaagaaaat tgcagtgttg gcggatatga aggaacttgg tgtccagtct
    61261 gttcaacttc ataatcagat gattttgagc ctttcgccag atgtgcttga tactgttatt
    61321 ttctacggag aagatattgc tgaattagcc caacttgcta gtcaaatgtt cccaatcgga
    61381 cacgtttatt acttcaagaa aacagaagac caagaccaat ttgaagacct agttaagcag
    61441 gtcaaagaaa gccttggggc tcatgaccaa atcctgctca aaggctctaa ctctatgaat
    61501 ctagccaagt tggtagaaag tttagaaaat gaagacaagt gattttgtca agtatttgca
    61561 aagaatgatt gccattacag atactggatt aacctttaca aaagatccgt ttgaccgtga
    61621 gcgctacgaa gacttgcgag accttctatc tgaaatgttg aatcaagcat cagaccttga
    61681 ttccgaagaa gtggcagaag tcttgaagcc aacttctgct tatgcgactc ccttaatgga
    61741 cgtccgtgct tggattgttg aggacgaaaa gatttgtctg gttaggggac aaggagagga
    61801 tagttgggct ttgccaggtg gctttggtga agtcggctat tctccaactg aaaatattct
    61861 caaggaaatt gaagaagaaa ccggttttaa agccaaagtt gaaagactgc tagctgtttt
    61921 tgataccaat cgtttccaac tacagagcaa acaatatgca aagtttgttt ttgaatgtaa
    61981 gcttctcgat ggacaattcc aagaaaatca agaaatcgct gaccttcaat tttttgcaat
    62041 tgacccactg ccgaatttat ctgaaaaacg cattaccaag gagcaaatcg agattctttg
    62101 gcaggtttat caaggtcaga gggagcaata tcttgactaa gaagatgatt atcgtatttc
    62161 taaattcatt tttcttaagt agcatggtat aataaaatgc aggaaaattt tgaatcatga
    62221 ggaagactag atgaatttat gggatatttt ctttacgact caggcaaccg agccgcccaa
    62281 atttgacctt ttttggtatg ttagcctatt tacgctctta gccttaacct tttatacagc
    62341 ccatcgctat cgtgaaaaga aggtttacca acgatttttc caaatcttgc agactgttca
    62401 gttaatcctt ctttatggtt ggtactgggt caatcatatg ccactgtcag aaagcctacc
    62461 cttttaccat tgccgtatgg ctatgtttgt ggtactcttg cttcctggtc agtccaaata
    62521 taaacaatac tttgcattat tgggaacatt tgggacatta gcagcctttg tttatccagt
    62581 gccagatgct tacccttttc cacatatcac cattctatcc tttatctttg gtcatttagc
    62641 actcttgggg aactctctag tttatctatt gagacagtat aatgcgcgat tgctggatgt
    62701 gaagggaatt tttctcatga cctttgccct aaatgccttg atttttgtgg tcaatttggt
    62761 gacaggtggc gattacggat ttttgacaaa accgccattg gttggggatc acggtctagt
    62821 agctaattat ttacttgttt caattgtgct ggtagctact atcagtttga ctaagaaaat
    62881 cttagaattc tttttagctc aagaagcaga aaaaatgatt gcaaaggaag cttaacacag
    62941 agctttcttt tttgctctta gagagttttt acaagcagct tataaaataa gaatttctga
    63001 atagacaaac tcaaaaaatg gctgggaaat ttaggaaaaa agcaagcacg attaaatttt
    63061 ttgtgttata atattttgtg aatagctatg cctatgttta gctatggaat aatacgaagt
    63121 gcgaaacttg gaagatagag aggaagcgat gtaatggcta gagaaggctt ttttacaggt
    63181 ctagatattg gaacaagctc tgtcaaggtg cttgtggccg agcagagaaa tggtgaatta
    63241 aatgtaattg gcgtgagtaa tgccaaaagt aaaggtgtaa aggatggaat tattgttgat
    63301 attgatgcag cagcaactgc tatcaagtca gccatttccc aagcggaaga aaaggcaggc
    63361 atttcgatta aatcagtaaa tgtcggcttg cctggtaatc ttttgcaggt agaaccaact
    63421 caggggatga ttccagtaac atctgatact aaggaaatta cggatcaaga tgttgaaaat
    63481 gttgtcaaat cagctttgac aaagagtatg acacctgacc gtgaagtcat tacctttatt
    63541 cctgaagaat ttattgtgga tggtttccaa gggattcgtg acccacgtgg catgatgggg
    63601 gttcgccttg aaatgcgtgg tttgctttat acaggacctc gtactatctt gcacaatttg
    63661 cgtaagacgg ttgagcgtgc aggtgttcag gttgaaaata ttatcatttc accactagca
    63721 atggttcagt ctgttttgaa tgaaggggaa cgtgaatttg gtgctacagt gattgatatg
    63781 ggggcaggtc aaacgactgt cgctacaatc cgtaatcaag aactccagtt cacacatatt
    63841 ctccaagaag gtggagatta tgtaactaaa gatatctcca aggttttgaa aacctctcgc
    63901 aaattagcgg aaggcttgaa actgaattac ggggaagcct atccgcctct tgcaagcaaa
    63961 gaaaccttcc aagtagaggt tattggagaa gtggaagcag tcgaagtgac ggaagcctac
    64021 ttgtcagaaa ttatttctgc acgaatcaag cacatccttg aacaaatcaa gcaagaatta
    64081 gatagaaggc gtctattgga cctccctggt ggtattgtct taatcggtgg gaatgccatt
    64141 ttaccaggta tggttgagct tgctcaggaa gtctttggcg tccgtgtcaa gctttatgtt
    64201 ccaaatcaag ttggtatccg taatccagcc tttgcgcatg tgattagttt atcagaattt
    64261 gcgggtcaat taacagaagt taatcttttg gctcagggag cgataaaagg tgagaatgac
    64321 ttaagtcatc agccaattag ttttggtggg atgctgcaaa aaacagctca gtttgtacaa
    64381 tcaacgcctg ttcaaccagc tcctgctcca gaagtagagt cggtggcgcc tacagaacca
    64441 atggcggatt tccaacaagc ttcacaaaat aaaccgaaat tagcagatcg tttccgtggc
    64501 ttgatcggaa gcatgtttga cgaataaaga ggaaaaataa attatgacat tttcatttga
    64561 tacagctgct gctcaagggg cagtgattaa agtaattggt gtcggtggag gtggtggcaa
    64621 tgccatcaac cgtatggtcg acgaaggtgt tacaggcgta gaatttatcg cagcaaacac
    64681 agatgtacaa gcattgagta gtacaaaagc tgagactgtt attcagttgg gacctaaatt
    64741 gactcgtggt ttgggtgcag gaggtcaacc tgaggttggt cgtaaagccg ctgaagaaag
    64801 cgaagaaaca ctgacggaag ctattagtgg tgccgatatg gtcttcatca ctgctggtat
    64861 gggaggaggc tctggaactg gagctgctcc tgttattgct cgtatcgcca aagatttagg
    64921 tgcgcttaca gttggtgttg taacacgtcc ctttggtttt gaaggaagta agcgtggaca
    64981 atttgctgta gaaggaatca atcaacttcg tgagcatgta gacactctat tgattatctc
    65041 aaacaacaat ttgcttgaaa ttgttgataa gaaaacaccg cttttggagg ctcttagcga
    65101 agcggataac gttcttcgtc aaggtgttca agggattacc gatttgatta ccaatccagg
    65161 attgattaac cttgactttg ccgatgtgaa aacggtaatg gcaaacaaag ggaatgctct
    65221 tatgggtatt ggtatcggta gtggagaaga acgtgtggta gaagcggcac gtaaggcaat
    65281 ctattcacca cttcttgaaa caactattga cggtgctgag gatgttatcg tcaacgttac
    65341 tggtggtctt gacttaacct tgattgaggc agaagaggct tcacaaattg tgaaccaggc
    65401 agcaggtcaa ggagtgaaca tctggctcgg tacttcaatt gatgaaagta tgcgtgatga
    65461 aattcgtgta acagttgttg caacgggtgt tcgtcaagac cgcgtagaaa aggttgtggc
    65521 tccacaagct agatctgcta ctaactaccg tgagacagtg aaaccagctc attcacatgg
    65581 ctttgatcgt cattttgata tggcagaaac agttgaattg ccaaaacaaa atccacgtcg
    65641 tttggaacca actcaggcat ctgcttttgg tgattgggat cttcgccgtg aatcgattgt
    65701 tcgtacaaca gattcagtcg tttctccagt cgagcgcttt gaagccccaa tttcacaaga
    65761 tgaagatgaa ttggatacac ctccattttt caaaaatcgt taagtaaatg aatgtaaaag
    65821 aaaatacaga acttgttttt cgagaagttg cagaggctag tctgagtgct catcgagaga
    65881 gtggttcggt ctctgtcatt gcagttacca agtatgtaga tgtaccgaca gcggaagcct
    65941 tgcttccgct aggtgttcat catatcggtg aaaatcgtgt agataagttt ctggaaaaat
    66001 atgaagcttt aaaagatcga gatgtgactt ggcatttgat tggtaccttg caaagacgta
    66061 aggtgaaaga tgtcattcaa tacgttgatt atttccatgc attggactca gtaaagctag
    66121 caggggaaat tcaaaaaaga agtgaccgag tcatcaagtg tttccttcaa gtaaatattt
    66181 ctaaagaaga aagcaaacac ggtttttcga gagaggaact gctggaaatc ttgccagagt
    66241 tagccagact agataagatt gaatatgttg gtttaatgac gatggcacct tttgaggcta
    66301 gcagtgagca gttgaaagag attttcaagg cgacccaaga tttacaaaga gaaattcaag
    66361 agaaacaaat tccaaatatg cctatgaccg agttaagtat gggaatgagt cgtgattata
    66421 aagaagcgat tcaattcggt tccacttttg ttcgtatagg tacatcattt tttaagtagg
    66481 agagaaccat gtctttaaaa gatagattcg atagatttat agattatttt acggaggatg
    66541 aggattcaag tctcccttat gaaaaaagag atgagcctgt gtttactcca gtaaattctt
    66601 cacaggaacc ggctctccca atgaatcaac cttcacagtc ggctggtaca aaagagaaca
    66661 atatcaccag acttcatgca agacaacagg aattggcaaa tcagagtcag cgtgcaacgg
    66721 ataaggtcat tatagatgtt cgttatccta gaaaatatga ggatgcaaca gaaattgttg
    66781 atttattggc aggaaacgaa agtatcttga ttgattttca gtatatgaca gaggtgcagg
    66841 ctcgtcgttg tttggactat ttggatggag cttgtcatgt tttagctgga aatttgaaaa
    66901 aggtagcttc taccatgtat ttgttgacac cagtgaatgt tattgtaaat gttgaagata
    66961 tccgtttacc agatgaagat caacagggtg agttcggttt tgatatgaag cgaaatagag
    67021 tacgataatg atttttttaa ttcgtatgat ttataatgca gtggatattt actccctgat
    67081 tttggtagcc ttcgctgtca tgtcttggtt tccaggtgcc tacgaatcca gtttaggtcg
    67141 ttggattgta gcgttggtga aaccagtgct tgctcccttg caacgcctgc ctttacagat
    67201 agcgggtctt gatttatctg tttgggtcgc gattgttttg gttcgatttt taggagaaaa
    67261 cctagtgcgt tttctggcga tgataggatg aataaaggga tttatcagca tttctccata
    67321 gaagatcgtc catttcttga caagggaatg gaatggataa agaaggtaga agatagctat
    67381 gctccttttt taactccttt tatcaatcct catcaggaga agctattaaa gattttggcc
    67441 aaaacctatg gtcttgcttg tagcagtagt ggggaattcg tctcgagtga gtatgttcga
    67501 gttttattat acccagatta tttccaacca gagttttcag attttgaaat atctctccag
    67561 gaaattgtgt attccaataa atttgaacat ttaacgcatg ctaagatttt agggacagtc
    67621 atcaatcaat tagggattga acggaaactt tttggagata tcctagtaga tgaagaacgg
    67681 gcgcagatta tgattaatca gcagtttctt cttctctttc aagatggact aaagaaaatt
    67741 ggtcgtatac ctgtttcgct ggaggaacgt cctttcaccg agaaaataga taagctagaa
    67801 cagtatcgag aactggattt atctgtgtct agttttcgat tagatgttct ttgttcaagt
    67861 tggagacttg attagtgtga gaaaatttgg tcgcttgaga ttacttcaag ataagggaca
    67921 aacgaaaaaa gagaagaaaa aaataaccgt ccagttatta ttaagtaagt gaggaataga
    67981 atgccaatta catcattaga aataaaggac aagacttttg gaactcgatt cagaggtttt
    68041 gatccagaag aagtcgatga atttttagat attgtggttc gtgattacga agatcttgtg
    68101 cgtgcgaatc atgataaaaa tttgcgtatt aagagtttag aagagcgttt gtcttacttt
    68161 gatgaaataa aagattcatt gagccagtct gtattgattg ctcaggatac agctgagaga
    68221 gtgaaacagg cggcgcatga acgttcaaac aatatcattc atcaagcaga gcaagatgcg
    68281 caacgcttgt tggaagaagc taaatataag gcaaacgaga ttcttcgtca agcaactgat
    68341 aatgctaaga aagtcgctgt tgaaacagaa gaattgaaga acaagagccg tgtcttccac
    68401 caacgtctca aatctacaat tgagagtcag ttggctattg ttgaatcttc agattgggaa
    68461 gatattctcc gtccaacagc tacttatctt caaaccagtg atgaagcctt taaagaagtg
    68521 gttagcgaag tacttggaga accgattcca gctccaattg aagaagaacc aattgatatg
    68581 acacgtcagt tctctcaagc agaaatggaa gaattacaag ctcgtattga ggtagccgat
    68641 aaagaattgt ctgaatttga agctcagatt aaacaggaag tggaaactcc aactcctgta
    68701 gtgagtcctc aagttgaaga agagcctctg ctcatccagt tggcccaatg tatgaagaac
    68761 cagaagtagc tccagtgcat ccgacaggtc caacaccagc tacagaaact gttgattcag
    68821 caccgggatt tgaagcaccg caagaatctg ttacaatttt ataagaaata ttctgagaac
    68881 aatatcttat ccttatattc ccagcgagca ggagatggtg tgagtcctgc attccctatc
    68941 gataagatta tcctctcaaa ctatcaagtc tgaatctagt aagatttgac gttccccacg
    69001 ttacgggata agagggagaa agactaaaac tttttccgaa taaaggtggt accacgattt
    69061 tcgtcctttt tggaagtcgt ggtttttaat ttgttattat ttataaagga gataccatga
    69121 aactcaaaga cacccttaat cttgggaaaa ctgaattccc aatgcgtgca ggccttccta
    69181 ccaaagagcc agtttggcaa aaggaatggg aagatgcaaa actttatcaa cgtcgtcaag
    69241 aattgaacca aggaaaacct catttcacct tgcatgatgg ccctccatac gctaacggaa
    69301 atatccacgt tggacatgct atgaacaaga tttcaaaaga tatcattgtt cgttctaagt
    69361 ctatgtcagg attttacgca ccatttattc ctggttggga tactcatggt ctgccaatcg
    69421 agcaagtctt gtcaaaacaa ggtgtcaaac gtaaagaaat ggacttggtt gagtacttga
    69481 aactttgccg tgagtacgct ctttctcaag tagataaaca acgtgaagat tttaaacgtt
    69541 tgggtgtttc tggtgactgg gaaaatccat atgtgacctt gactcctgac tatgaagcag
    69601 ctcaaattcg tgtatttggt gagatggcta ataagggtta tatctaccgt ggtgctaagc
    69661 cagtttactg gtcatggtca tctgagtcag cccttgctga agcagagatt gaataccatg
    69721 acttggtttc aacttccctt tactatgcca acaaggtaaa agatggcaaa ggagttctag
    69781 atacagatac ttatatcgtt gtctggacaa cgactccatt tactatcaca gcctctcgtg
    69841 gtttgacggt tggtgcagat attgattacg ttttggttca acctgctggt gaagctcgta
    69901 agtttgtcgt tgctgctgaa ttattgacta gcttgtctga gaaatttggc tgggctgatg
    69961 ttcaagtttt ggaaacttac cgtggccaag aactcaacca catcgtaaca gaacacccat
    70021 gggatacagc tgtagaagag ttggtaattc ttggtgacca cgttacgact gactctggta
    70081 caggtattgt ccatacagcc cctggttttg gtgaggacga ttacaatgtt ggtattgcta
    70141 ataatcttga agtcgcagtg actgttgatg aacgtggtat catgatgaag aatgctggtc
    70201 ctgaatttga aggtcaattc tatgaaaagg tagttccaac tgttattgaa aaacttggta
    70261 acctccttct tgcccaagaa gaaatctctc actcatatcc atttgactgg cgtactaaga
    70321 aaccaatcat ctggcgtgca gttccacaat ggtttgcctc agtttctaaa ttccgtcaag
    70381 aaatcttgga cgaaattgaa aaagtgaaat tccactcaga atggggtaaa gtccgtcttt
    70441 acaatatgat ccgtgaccgt ggtgactggg ttatctctcg tcaacgtgct tggggtgttc
    70501 cacttcctat cttctacgct gaagatggta cagctatcat ggtagctgaa actattgaac
    70561 acgtagctca actttttgaa gaacatggtt caagcatttg gtgggaacgt gatgccaaag
    70621 acctcttgcc agaaggattt actcatccag gttcaccaaa cggcgagttc aaaaaagaaa
    70681 ctgatatcat ggacgtttgg tttgactcag gttcatcatg gaatggagtg gtggtaaacc
    70741 gtcctgaatt gacttaccca gccgaccttt acctagaagg ttctgaccaa taccgtggtt
    70801 ggtttaactc atcacttatc acatctgttg ccaaccatgg cgtagcacct tacaaacaaa
    70861 tcttgtcaca aggttttgcc cttgatggta aaggtgagaa gatgtctaaa tctcttggaa
    70921 ataccattgc tccaagcgat gttgaaaaac aattcggtgc tgaaatcttg cgtctctggg
    70981 taacaagtgt tgactcaagc aatgacgtgc gtatctctat ggatatcttg agccaagttt
    71041 ctgaaactta ccgtaagatt cgtaacactc ttcgtttctt gattgccaat acatctgact
    71101 ttaacccagc tcaagataca gtcgcttacg atgagcttcg ttcagttgat aagtacatga
    71161 cgattcgctt taaccagctt gtcaagacca ttcgtgatgc ttacgcaaac tttgaattct
    71221 tgacaatcta caaggccttg gtgaacttta tcaacgttga cttgtcagcc ttctaccttg
    71281 attttgccaa agatgttgtt tacattgaag gtgccaaatc actggaacgc cgtcaaatgc
    71341 agactgtctt ctatgacatt cttgtcaaaa tcaccaaact cttgactcca atccttcctc
    71401 acactgcgga agaaatctgg tcatatcttg agtttgaagc cgaagacttc gtccaattgt
    71461 cagaattacc agaagttcaa acttttgcta accaagaaga aatcttggat acatgggcag
    71521 ccttcatgga cttccgtgga caagcacaaa aagccttgga agaagctcgt aatgcaaaag
    71581 ttatcggtaa atcacttgaa gcacacttga cagtttatcc aaatgaagtt gtgaaaactc
    71641 tactcgaagc agtaaacagc aatgtagctc aacttttgat cgtgtctgag ttgaccatcg
    71701 cagaagaacc agctccggaa gctgccctta gcttcgaaga tgtagtcttc acagttgaac
    71761 gcgctgcagg tgaagtatgt gaccgttgcc gtcgtattga cccaacaaca gcagaacgta
    71821 gctaccacgc agtcatctgt gaccactgtg caagtatcgt agaagaaaac tttgcagatg
    71881 cagtcgcaga aggatttgaa gagaaataag attgaaaagt ctaggtaaaa ctcaatttga
    71941 gaagaaaaga caactaattt tatagtctat taaacgcatt gtatcacgtt tttgaacacc
    72001 tgatatgatg cgttttttat ttattttaaa aatttgcgag gtatgacttt ttatactcaa
    72061 caagaatcaa agagaaactt agcaagctaa cagtagtaat ataaaatagg aatttgatat
    72121 tagggataag attggtaaat agtgtaatat ttttacaaca ataaatttat atagttattt
    72181 ctggtttctg aaaagtatta tattatattt tatttcatat tatacaaatt tttattttat
    72241 aatatcagaa aatacttttt ttaaaaagca aatatgatac aattttattt gaaaaaaaat
    72301 aaaaaaggag attttattat gaaatcaagt ggtatcgtcg gtcttgtggg aggaatttta
    72361 cttcttattt gtccttttgt cttgttggga acagcggtaa atacagctgc tacaactctt
    72421 aatggaggag ctactgcagg ggctttttca ggtgttgcct tactcttgaa tgccttgaag
    72481 attgcaaatc ttgttcttgg tatcattgct attgtttact ataaaggaga taagcatgta
    72541 ggtgcagctc cgtctgtact aatgattgtt tctggtgaag ttagtctcat tccattctta
    72601 ggatgggttg gggggattct tgctattatc ggaggatctc tattccttgc aacattgaag
    72661 aaattcaaat cagaagaata aaaggtattt tagcatgaaa agaacaaaaa agtttatcgg
    72721 tataggagta gctctattat ctctttctct tctagttgca tgtggaacat aaagttcaaa
    72781 gaatacttca acaagtaatg atgagaagac agtagcaaca tccaatagtt caaaagaaac
    72841 aatcactttc gatacaccgg ttgtaacaga cgatgcgatt gaatcaatac gcacttatgc
    72901 agattatata gatctttata aaaatatttt tgatgattat tttactaaag ctgaggaagg
    72961 tttcaaaggc acaactatgg aaaataatga ctcgtttact aaactaaaag agtcaactca
    73021 aaaattattc gatgcgcaga aaaaaaggtt aaataatgaa gatagaatag aaacaaccaa
    73081 aaacaatgtg attgccaaac attgtcaaac agtcctttcc tttttggttt tgactagctt
    73141 ttttgtgaaa aattgtgtaa aatagaatag ataaacgagg ggaaacctcg gaaaatttaa
    73201 aggagaatcc atctaatggt aaaattggtt tttgctcgcc acggtgagtc tgaatggaac
    73261 aaagctaacc ttttcactgg ttgggctgat gttgatttgt ctgaaaaagg tacacaacaa
    73321 gcgattgacg ctggtaaatt gatcaaagaa gctggtatcg aatttgacca agcttacact
    73381 tcagtattga aacgtgctat caaaacaact aacttggctc ttgaagcttc tgaccaattg
    73441 tgggttccag ttgaaaaatc atggcgcttg aacgaacgtc actacggtgg tttgactggt
    73501 aaaaacaaag ctgaagctgc tgaacaattt ggtgatgagc aagttcacat ctggcgtcgt
    73561 tcatacgatg tattgcctcc aaatatggac cgtgatgatg agcactcagc tcacacagac
    73621 cgtcgttatg cttcacttga cgactcagtc attccagatg ctgaaaactt gaaagtgact
    73681 ttggaacgtg ctcttccatt ctgggaagat aaaatcgctc cagctcttaa agatggtaaa
    73741 aacgtattcg taggagctca cggtaactca atccgtgccc ttgtaaaaca catcaaaggt
    73801 ttgtcagatg atgagatcat ggacgtggaa atccctaact tcccaccatt ggtattcgaa
    73861 ttcgacgaaa aattgaacgt tgtttctgaa tactaccttg gaaaataaaa aattgtaagc
    73921 ctaggattga tttctaggct ttttatgtta gtatggaagt atgataagga ataaaaaaca
    73981 agattatgta ctggcctaca agcaaccagc ttcaaccact tacatgggtt gggaagaaga
    74041 agctttaccg ataggcaatg gttctttagg agcaaaagta tttggcctta taggggctga
    74101 acggattcaa tttaatgaaa aaagtctctg gtctggaggt ccacttcctg atagttcaga
    74161 ttatcagggt ggaaatcttc aggatcagta tgttttttta gctgagattc ggcaggcttt
    74221 ggagaagaga gattacaatc tggctaagga actggctgag cagcacctaa ttgggccaaa
    74281 aacgagtcaa tatgggacct atctgtcttt tggggatatt cacattgagt tcagccagca
    74341 aggtacgact ttgtctcagg tgacggacta tcagagacag ctgaatatta gtaaggcact
    74401 tgcgacgact tcttatgtct ataagggaac gcgatttgaa cgtaaagctt ttgcgagttt
    74461 tccagatgat ctcttgattc aatgttttac taaggaaggg ttggaaactc tagattttac
    74521 tatagaacta tccttgacct gtgatttggc ttctgatgga aagtatgagc aggaaaaatc
    74581 tgattacaag gagtgtaagt tggatattac tgattctcat atcttgatga agggaagagt
    74641 taaggataat gatctgcggt ttgctagtta tctagcttgg gaaacggatg gagatattag
    74701 agtttggtca gatagggttc agatatcagg agccagttat gccaatctct tcttggccgc
    74761 taagacggat tttgcccaaa atcctgctag caattatcgc aagaaactag atttagagca
    74821 acaggtgata gacttggtgg acacagctaa agaaaagggc tatacccaat tgaaatcaag
    74881 gcatatcgag gactaccaag ccttattcca gcgtgttcaa ttggatttgg aagctgatgt
    74941 tgacgcatcc actacagatg atttgttaaa aaattataag ccacaagaag ggcaggcttt
    75001 ggaggagctg ttcttccagt atggacggta tttattgatt agttcgtcca gagactgccc
    75061 agatgctcta ccagctaacc tacagggagt ctggaatgcg gtcgacaatc ctccttggaa
    75121 ttcggactat cacttaaatg tcaatctgca gatgaattat tggccagcct atgttaccaa
    75181 tctcctagag acggtctttc cagtcatcaa ctatgtagat gatttgcgtg tctatggtcg
    75241 tctagcggct gtaaagtatg caggaatcgt ctctcaaaaa ggtgaggaaa atggttggtt
    75301 ggttcatact caagcgactc cctttggttg gacggcacct ggttgggatt actattgggg
    75361 ttggtcacca gctgccaatg cgtggatgat gcaaaccgtt tatgaagcct attcatttta
    75421 tagggaccaa gactatctca gggagaaaat ttatcccatg ttgagggaaa cggttcgttt
    75481 ttggaatgcc tttttacata aggatcagca ggcgcagcgt tgggtctctt ctccgtctta
    75541 ttccccagaa catgggccga tttcgattgg caatacctat gaccaatctc tgatttggca
    75601 gttatttcat gattttattc aggctgctca ggaattggga ctggatgagg acttgttgac
    75661 tgaggttaag gagaagtctg atttactaaa tcctttgcaa atcactcaat ctggtcgaat
    75721 cagggagtgg tatgaggagg aagagcagta ttttcaaaat gagaaagtgg aggcccagca
    75781 tcggcacgct tcccatctag tgggactcta ttctggcaat ctctttagct acaagggaca
    75841 agagtatatt gaagcggcgc gtgctatcct caatgatcgt ggagatggcg gcacaggctg
    75901 gtccaaggct aataagatca atctctgggc gcgtttggga gatggcaatc gagcccataa
    75961 attattggca gagcagttaa agacatccac cttgccaaat ctttggtgta gccatcctcc
    76021 ttttcagata gatggtaatt ttggtgctac tagtggcatg gcagaaatgt tactccagtc
    76081 tcatgcagct tatctggtac ctctagctgc cctacctgat gcttggtcaa caggttctgt
    76141 ttcaggctta atggcacgtg gacattttga agtgagcatg agctgggaag ataaaaaact
    76201 cttacagttg accattttat caaggagtgg aggagatttg cgagtttctt atccagatat
    76261 tgagaagagt gtgattaaaa tgaatcaaga aaaaataaaa gcgaaatgca tggggaaaga
    76321 ttgtatttcg gtggcaacag cagaaggtga tcttgttcaa ttttattttt aagaagatgt
    76381 tataaggcag taatttgaaa cttcctttta ataaggattt gagaatatag gcagtttcca
    76441 actagttgaa aaagcgttat aatgataata ggaagtaata ctcaatgaaa atcaaagagc
    76501 acaaactagg aagctagccg caggttgctc aaaacagtgt tttgaggttg tagatggaag
    76561 ctgacgtggt ttgaagagag attttcgagg agtataattt gtttgataga gggtgggtct
    76621 gatggcttat attgagatga aacactgtta caagcgttat caggttgggg acacggagat
    76681 tgtggccaat cgtgatgtga attttgagat tgaaaagggg gagctggtta ttatccttgg
    76741 tgcttcaggt gcaggcaagt caacagttct taaccttctt gggggaatgg ataccaatga
    76801 tgaaggggaa atctggattg atggtgttaa tattgcggat tatagttccc accagcgtac
    76861 caattaccgt agaaatgatg tggggtttgt ttttcagttt tataatctag tttctaatct
    76921 gacagctaag gaaaatgtgg aactggcttc tgaaattgtg acagatgcct tgaatcctga
    76981 tcaggtcttg acagatgtag gtctggctca tcgtctcaat aactttccag cccagctttc
    77041 tggaggggag caacagcgag tctccattgc acgcgcggta gccaaaaatc ctaaaattct
    77101 cctttgtgat gaaccgactg gagccttgga ttatcagacg ggcaagcagg ttttgaaaat
    77161 tctccaagac atgtctcgtc aaaagggagc gacggtgatc atcgtgactc ataatggagc
    77221 tttggcgccc attgctgatc gcgtgattca aatgcacgat gccagtgtca aggatgtggt
    77281 gctcaaccag catcctcagg atattgacag tttggagtac tagcatgatc aagcgaaaaa
    77341 cttattggaa ggacttaatt cagtccttca caggctccaa ggggcgtttt ttatccatct
    77401 tgatcctgat gatgttggga tctctagcct tagtaggcct caaagtaacc agtcccaaca
    77461 tggaggcgac agctaatgct tatttaacaa ctgctcaaac cttggatttg gcagtcatgt
    77521 ctaactatgg cttggatcaa gcagaccaag aagaactaaa acagacggag ggcgcagagg
    77581 tcgagtttgg ctatttgaca gatgtgacta tggataatgg gcaggatgcc attcggctgt
    77641 actccaaacc agagcgaatt tcaacctttc agctaagaaa gggacgactt cctcagtcag
    77701 acaaggaaat cgctttggcc actcatttgc aaggccaata cagcgtggga caggagatta
    77761 gttttaaaga aaaagaagag ggtcattcct ctttaaaaga ccatacttat accattactg
    77821 gttttgtgga ttcggctgaa atcctctccc agcgagatat gggctacgca ggaagtggaa
    77881 gtgggactct gacagcctat ggggtgattt tacctagtca gtttgatcag aaagtctaca
    77941 atatagctcg tttgaaatat caagatttag caggtttaaa tgccttttca tcagcttatg
    78001 aagaaaaatc caagcaacat caggaagacc ttgaacaaac tttatcagat aatggcaagg
    78061 tacgtctgca acttttgaaa aaagaaggac aagagtctct agacaagggg caagagaccc
    78121 ttgacaaggc tcagactaat ttgcaggaag gcaagcgtcg tttagcagct gctcaagctc
    78181 gtatacaggc tcaagaaagt caactagcct tgtttcctca agttcagaga gagcaggcta
    78241 gtgctcaact tacccaagcc aagcaggaat tgggcaagga agaggataaa ctaaagcaag
    78301 ctgaacaaaa tctagcccaa gaaaaggaaa aattagaaaa acatcagcaa gtcttggacg
    78361 atttggcgga gccaaggtat caggtctata atcgtcagac catgccaggt ggtcagggct
    78421 atcttatgta tagcaatgct tcatccagta ttcgagcagt gggcaatatc tttcctgtgg
    78481 tactttatgc cgtagcatcc atggtgacct ttacgaccat gactcgcttt gtagacgaag
    78541 agcgaactca tgcagggatt tttaaggcct tgggttaccg tagtaaggat attatcacca
    78601 agtttctcct ttatggacta gtagctggga ctgtcggaac ggctctaggt agtatacttg
    78661 gtcattattt gctagccagt gtaatttcaa gtgtcattac aaaaggcatg gtggtgggag
    78721 aaacccagat tcagttctat tggacctata gcttactagc tcttgtcttg agctggttgg
    78781 cgagtgtgtt accagcctat ctggtggctt ggagggaact tcatgccgaa gcagcccagc
    78841 ttctacttcc taaacctcct gtcaaaggag ctaaaatctt attggagcgt atcggtttta
    78901 tctggcgtcg tctcagtttt actcataagg taacagcccg caacatcttt cgttataagc
    78961 agagaatgtt gatgacaatc tttggtgtgg caggttctgt agctctgctc tttgcaggtt
    79021 tgggaatcca atcttctgta gcaggagttc cgtctaaaca gtttcaacaa atccaacagt
    79081 atcagatgct tgtctctgaa aatcctagtg cgaccaatca ggacaaggta gagctagcag
    79141 aagtgttgaa agggcaggag atactagcct accagaaaat ctattctaaa acgctagaca
    79201 aggatttcaa aggcaaggct ggtcttcaaa acattactct tatgatgata gagaaggaag
    79261 atttgactcc ctttatccat cttcaacatc atcagcagga gctgacatta aaagatggca
    79321 tcgttattac agctaaactc gcccagctgg caggtgtcaa ggttgggcag actttagaaa
    79381 ttgaaggtaa ggaactaaag gtcgctgcta ttactgagaa ctacgttggt cactttattt
    79441 atatgagtca ggctagctat gagcaacttt acggacagct accccaagcc aacacttatc
    79501 tggtctcatt aagggatacc agtgcaacta gtatcgaaag tcaggcgggc ttgcttatga
    79561 atcaatctgc ggtgtccagc gttgtccaaa atgcttcagc cattcgactc tatcgctagc
    79621 tcactcaatc agaccatgac catcttggtc atcgtatcag ttctattagc tattgtcatc
    79681 ctttacaatc tgaccaatat caacgtagct gagagaatcc gtgaactctc cactatcaag
    79741 gttctcggtt ttcataataa tgaagtcacc ctctacattt accgtgagac gattgtgctg
    79801 tcccttgtgg gaatcgtact tggtctggta gctggtttct atttacacca atttttgatt
    79861 caaatgattt cgcctgcgac tattctcttt tatccgcagg taggctggga agtctatgta
    79921 atcccagtgg caacagtaag catcattttg accttgcttg gtttcttcgt caattattat
    79981 ctgagaaagg ttgatatgtt agaagccctg aaatctgtag agtaaggtag ttatttttag
    80041 ctgattgaac ttctatttac taatattcaa aaatcctctg tcacaaagag cagggagctc
    80101 tttgtgacag aggatttttt ctatagggct ttagcagctg caattgcggc ttcgaagttt
    80161 ggctcagaat tgatattatc cacgtattca acgtagcgaa tcgtattgtc agtatcgagg
    80221 acaaagactg cgcgtgctaa taggtgccat tcgttgatca agagggcata atcgcgcccg
    80281 aaagaatggt caaagtagtc tgaaagcata atggcattgt caaggccttc agcaccgcac
    80341 caacgttttt gagcaaaagg taggtccatt gaaacagtca atacgaccgt gttgtccagt
    80401 ccagccaatt cttcattaaa acgacgtgtt tgagttgagc agatgcctgt atcgatagaa
    80461 ggaacgacac tcaagacttt tttcttgcca tcaaaatcag ccagagattt tttagaaaga
    80521 tctgttgtag taagagaaaa atcaagcgcc ttgtcgccga cttgtagttg tttacctgta
    80581 aagctcacag gatttccgag aaaagttacc ataggatact ccaatctttt ttcttccatt
    80641 gtatctgaaa cagtcagaat tttccaatga tttgaccgga aatgtgggca tagaaaaaac
    80701 gccagctcac atgagaatga cgtttttcag aggcttattt tgccaatcct tcagcaatct
    80761 tgtcaaggtt gtatttcatc atgctgtagt agctgtcgcc ttctttacct tgttctgcga
    80821 tagagtcagt aaagatttgt gcgtagattg ggatgtttgt gtcttgagaa acagttttca
    80881 ttggacggtc atccacactt gattctacaa agagtgatgg aacttttgtt tggcgaagtt
    80941 tttcaaccaa ggtcttgatt tgttcaggag ttccttcttc ttcagtattg atttcccaga
    81001 tgtaagcact tgggacacca taggctttag agaagtattt gaatgctcct tcgctggtta
    81061 caatgagttt cttttcagca gggatcttat taaatttatc cttactttct ttatcaagtt
    81121 tgtctaactt atcagtatat tctttgagat ttttttcata gaattcttta ttgttagggt
    81181 ctttggcgct caattgtttg gcgatatttt tagcaaaaat aataccgttt tcaaggttaa
    81241 gccaagcgtg tgggtcttct tttccttttt cattttgacc ttcaaggtag ataacatcaa
    81301 cgccgtcgct gactgcgaag tagtctttgt tttcagtttt cttggcattt tctaccaatt
    81361 ttgtaaacca agcattgcca cctgtttcaa ggttgatacc gttatagaaa atcaaatcag
    81421 cctcagaagt tttcttaacg tcttcaggaa gtggttcgta ttcgtgtggg tcttgcccaa
    81481 tcggaacgat actatgaagg tcaattttgt caccagcaat atttttagta atatcagcga
    81541 tgattgagtt tgtagcaaca acttttagtt tttgaccaga agttgtatct ttttttccgc
    81601 tagcacatgc tacaagaatg attgcagaaa gaaagagaac gagtaatgta cctaattttt
    81661 tcattagatc ctccaaatta ttagggcttt gccccttatt ttaacaaatg tttatttttc
    81721 agtttcaaat atcgttgttt gggagcgata aagaagctaa tgagaaagaa actagcagct
    81781 gtaagcacga tactagaacc tgccgcaaca ttaaaactat agccaataaa gagtcccaaa
    81841 actgaagcag tagctccgaa ggttgaggaa aggaaaatca tgcttttcag actattagca
    81901 tacagataag cagttgcagc tggggtaatc agcatggcta caatcaggat agttccgaca
    81961 ctttgcatgg ctgtcacaga cacgagagtc aggagtacca tgagaaggta gtgatagaaa
    82021 ttgacaggca ttcccatggc tttagccaag agttcatcaa aggaagttat caagagttgc
    82081 ttgaagaaaa tccagattaa caagaggatg gctgccccca cacccatagt aataaacata
    82141 tccgtatctt ggacggccag gatattacca aaaaggatat ggaaaaggtc agttgaactt
    82201 ttagcgacac caatcaagat gataccgagg gctaagaaag aagaaaaggt aatgccgatg
    82261 gcggtatcgc ttttgataat cgagtttcct ttgatgtagg taatgatgat ggcagctagc
    82321 aatccaaaga caatggctcc gataaagaag tcaaggccca agatgaagga gagggctaca
    82381 cctggtaaga cagcatgtga aatggcatct cccatgagtg acatcccgcg tagaataatg
    82441 aaacatccca cagctccagc tacaatcccg acgacaatag ctgttatcaa ggcattttgt
    82501 aggaaatgga atttttgcaa tccatcgata aattctgcaa tcataggtca cctccattga
    82561 aaaagagttg attaccgtaa gcttctttta gattggtttc ggtaaaagtt tcttttgttg
    82621 gaccaaaggc aatcacttct cgattgacaa gtaagacttg atcgaagtag tggggaatct
    82681 tgctgaggtc gtggtgaacg atgagaaccg tcttcccagc ttttttcaaa tctctcagcg
    82741 tattcatgat gatttcctca ctgacagagt caatcccagc aaagggttca tccaagagga
    82801 tatagtcggc ttcctgcacc aaacatctgg caatcaaaac ccgctggaat tgacctccag
    82861 acagttgact aatttgacgt tcagcgtagt cagctaggcc gacgatttca agggcctctt
    82921 gcactttctt ccaatgttta gcctttaaac ttcgaaagag aggaatagag ggaaatagtc
    82981 ctaacgagac gcattccttg accttgatgg gaaagttgta gtcgatattg attttttgtt
    83041 cgacataggc aattcggtgt aaggattttt taacttcctt gtcatcgaga aatgcctgac
    83101 cttgatgtgg gataattccc agcatacctt ttaatagtgt tgatttccca gcgccgtttg
    83161 gaccaatgat gccggtaatt gttggtccat ggagcactag tgaaatatcc ttaagtgcca
    83221 acgtttcttt gtaggagaca ctgaggtttt cgatacgtat cataaacttg tattcctcct
    83281 gtctcttaat atacattaaa aaaaaaatta agtcaagtta atttttgaaa aaattaaaat
    83341 aataactgaa aaataga
//
LOCUS       DAVBCB010000002        78793 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVBCB010000002 DAVBCB010000000
VERSION     DAVBCB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 78793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.002"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(48..1937)
                     /locus_tag="VQU28_000077"
     CDS             complement(48..1937)
                     /locus_tag="VQU28_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HET2475514.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFEKDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDQAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSLFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2075..3640
                     /locus_tag="VQU28_000078"
     CDS             2075..3640
                     /locus_tag="VQU28_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2475515.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKKPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKNDNRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMCKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3640..4404
                     /locus_tag="VQU28_000079"
     CDS             3640..4404
                     /locus_tag="VQU28_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2475516.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQARPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4603..4818)
                     /locus_tag="VQU28_000080"
     CDS             complement(4603..4818)
                     /locus_tag="VQU28_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475517.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
     gene            complement(5689..6939)
                     /gene="ilvA"
                     /locus_tag="VQU28_000081"
     CDS             complement(5689..6939)
                     /gene="ilvA"
                     /locus_tag="VQU28_000081"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HET2475518.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(6989..7345)
                     /locus_tag="VQU28_000082"
     CDS             complement(6989..7345)
                     /locus_tag="VQU28_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2475519.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7583..7819)
                     /locus_tag="VQU28_000083"
     CDS             complement(7583..7819)
                     /locus_tag="VQU28_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475520.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKH"
     gene            complement(7926..8948)
                     /gene="ilvC"
                     /locus_tag="VQU28_000084"
     CDS             complement(7926..8948)
                     /gene="ilvC"
                     /locus_tag="VQU28_000084"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HET2475521.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVVEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9014..9490)
                     /gene="ilvN"
                     /locus_tag="VQU28_000085"
     CDS             complement(9014..9490)
                     /gene="ilvN"
                     /locus_tag="VQU28_000085"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HET2475522.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9483..11183)
                     /locus_tag="VQU28_000086"
     CDS             complement(9483..11183)
                     /locus_tag="VQU28_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HET2475523.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11384..13051)
                     /locus_tag="VQU28_000087"
     CDS             complement(11384..13051)
                     /locus_tag="VQU28_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HET2475524.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13054..13419)
                     /locus_tag="VQU28_000088"
     CDS             complement(13054..13419)
                     /locus_tag="VQU28_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2475525.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13576..13764)
                     /locus_tag="VQU28_000089"
     CDS             complement(13576..13764)
                     /locus_tag="VQU28_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HET2475526.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(13880..14056)
                     /locus_tag="VQU28_000090"
     CDS             complement(13880..14056)
                     /locus_tag="VQU28_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2475527.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(14167..14316)
                     /locus_tag="VQU28_000091"
     CDS             complement(14167..14316)
                     /locus_tag="VQU28_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HET2475528.1"
                     /translation="MDKVETTIKSLCVHHYNLKFYVFNDDLPREWFQLMEKRLETLNS
                     EIVNV"
     gene            complement(14628..16172)
                     /locus_tag="VQU28_000092"
     CDS             complement(14628..16172)
                     /locus_tag="VQU28_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HET2475529.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMNPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16385..16687
                     /gene="gatC"
                     /locus_tag="VQU28_000093"
     CDS             16385..16687
                     /gene="gatC"
                     /locus_tag="VQU28_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HET2475530.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEQDNYYIKVPAILDDGGDA"
     gene            16687..18153
                     /gene="gatA"
                     /locus_tag="VQU28_000094"
     CDS             16687..18153
                     /gene="gatA"
                     /locus_tag="VQU28_000094"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HET2475531.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDANNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDTKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGIDPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18153..19595
                     /gene="gatB"
                     /locus_tag="VQU28_000095"
     CDS             18153..19595
                     /gene="gatB"
                     /locus_tag="VQU28_000095"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HET2475532.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19724..20284
                     /gene="efp"
                     /locus_tag="VQU28_000096"
     CDS             19724..20284
                     /gene="efp"
                     /locus_tag="VQU28_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HET2475533.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            20306..20695
                     /locus_tag="VQU28_000097"
     CDS             20306..20695
                     /locus_tag="VQU28_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2475534.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGVYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            20688..21110
                     /gene="nusB"
                     /locus_tag="VQU28_000098"
     CDS             20688..21110
                     /gene="nusB"
                     /locus_tag="VQU28_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HET2475535.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTNVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKVGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(21159..21536)
                     /locus_tag="VQU28_000099"
     CDS             complement(21159..21536)
                     /locus_tag="VQU28_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2475536.1"
                     /translation="MVLSNGQPTFLSTILSFVLLVIIGPVFEETLDRGYFMNTFFPKS
                     KYYLDVILSGLIFGISHLILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFF
                     NFLNHAKPIWIFVYNYIYYHFFR"
     gene            complement(21877..22062)
                     /gene="briC"
                     /locus_tag="VQU28_000100"
     CDS             complement(21877..22062)
                     /gene="briC"
                     /locus_tag="VQU28_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HET2475537.1"
                     /translation="MTGTETFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(22221..22988)
                     /locus_tag="VQU28_000101"
     CDS             complement(22221..22988)
                     /locus_tag="VQU28_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HET2475538.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(22985..23851)
                     /locus_tag="VQU28_000102"
     CDS             complement(22985..23851)
                     /locus_tag="VQU28_000102"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HET2475539.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(23888..25255)
                     /gene="accC"
                     /locus_tag="VQU28_000103"
     CDS             complement(23888..25255)
                     /gene="accC"
                     /locus_tag="VQU28_000103"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HET2475540.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(25267..25689)
                     /gene="fabZ"
                     /locus_tag="VQU28_000104"
     CDS             complement(25267..25689)
                     /gene="fabZ"
                     /locus_tag="VQU28_000104"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HET2475541.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(25686..26171)
                     /locus_tag="VQU28_000105"
     CDS             complement(25686..26171)
                     /locus_tag="VQU28_000105"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HET2475542.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(26174..27409)
                     /gene="fabF"
                     /locus_tag="VQU28_000106"
     CDS             complement(26174..27409)
                     /gene="fabF"
                     /locus_tag="VQU28_000106"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HET2475543.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(27431..28162)
                     /gene="fabG"
                     /locus_tag="VQU28_000107"
     CDS             complement(27431..28162)
                     /gene="fabG"
                     /locus_tag="VQU28_000107"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HET2475544.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(28196..29116)
                     /gene="fabD"
                     /locus_tag="VQU28_000108"
     CDS             complement(28196..29116)
                     /gene="fabD"
                     /locus_tag="VQU28_000108"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HET2475545.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(29109..30083)
                     /gene="fabK"
                     /locus_tag="VQU28_000109"
     CDS             complement(29109..30083)
                     /gene="fabK"
                     /locus_tag="VQU28_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HET2475546.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(30202..30426)
                     /locus_tag="VQU28_000110"
     CDS             complement(30202..30426)
                     /locus_tag="VQU28_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2475547.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(30486..31460)
                     /locus_tag="VQU28_000111"
     CDS             complement(30486..31460)
                     /locus_tag="VQU28_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HET2475548.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANNRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(31460..31894)
                     /locus_tag="VQU28_000112"
     CDS             complement(31460..31894)
                     /locus_tag="VQU28_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2475549.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(31988..32773)
                     /locus_tag="VQU28_000113"
     CDS             complement(31988..32773)
                     /locus_tag="VQU28_000113"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HET2475550.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            33098..34462
                     /locus_tag="VQU28_000114"
     CDS             33098..34462
                     /locus_tag="VQU28_000114"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HET2475551.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            34465..34836
                     /locus_tag="VQU28_000115"
     CDS             34465..34836
                     /locus_tag="VQU28_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HET2475552.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            35050..36324
                     /gene="serS"
                     /locus_tag="VQU28_000116"
     CDS             35050..36324
                     /gene="serS"
                     /locus_tag="VQU28_000116"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HET2475553.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGTDEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSAAKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(36349..37248)
                     /locus_tag="VQU28_000117"
     CDS             complement(36349..37248)
                     /locus_tag="VQU28_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HET2475554.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACSGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPALSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(37358..37906)
                     /locus_tag="VQU28_000118"
     CDS             complement(37358..37906)
                     /locus_tag="VQU28_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HET2475555.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(38124..39446)
                     /locus_tag="VQU28_000119"
     CDS             complement(38124..39446)
                     /locus_tag="VQU28_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HET2475556.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVFFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(39537..39625)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(39789..42125)
                     /locus_tag="VQU28_000120"
     CDS             complement(39789..42125)
                     /locus_tag="VQU28_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HET2475557.1"
                     /translation="MNKKILETLEFDKVKALFDPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(42229..42777)
                     /locus_tag="VQU28_000121"
     CDS             complement(42229..42777)
                     /locus_tag="VQU28_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HET2475558.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIFYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(42774..43076)
                     /gene="zapA"
                     /locus_tag="VQU28_000122"
     CDS             complement(42774..43076)
                     /gene="zapA"
                     /locus_tag="VQU28_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HET2475559.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            43163..44044
                     /locus_tag="VQU28_000123"
     CDS             43163..44044
                     /locus_tag="VQU28_000123"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HET2475560.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            44048..44662
                     /gene="lepB"
                     /locus_tag="VQU28_000124"
     CDS             44048..44662
                     /gene="lepB"
                     /locus_tag="VQU28_000124"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HET2475561.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            44721..44864
                     /locus_tag="VQU28_000125"
     CDS             44721..44864
                     /locus_tag="VQU28_000125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="chlorohydrolase"
                     /protein_id="HET2475562.1"
                     /translation="MFIDKYSTKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVA
                     DGS"
     gene            44941..47307
                     /locus_tag="VQU28_000126"
     CDS             44941..47307
                     /locus_tag="VQU28_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HET2475563.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLSKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTHLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(47355..48638)
                     /locus_tag="VQU28_000127"
     CDS             complement(47355..48638)
                     /locus_tag="VQU28_000127"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HET2475564.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     EDGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(48832..49191)
                     /locus_tag="VQU28_000128"
     CDS             complement(48832..49191)
                     /locus_tag="VQU28_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2475565.1"
                     /translation="MDSPKSLASYRVIGISDRVLEILKEYLLLEEGDYIFTNNGSPLM
                     ITCFNYFLKNRFRKSEIKKDDFVLTAHVFRYSHISLLAELEVPITAIMDRVDYTNETK
                     ILSVYTHVTEKMKSNIT"
     gene            complement(49490..50119)
                     /locus_tag="VQU28_000129"
     CDS             complement(49490..50119)
                     /locus_tag="VQU28_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2475566.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPTVSPSQEPFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYPSYNFKVHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(50164..50403)
                     /locus_tag="VQU28_000130"
     CDS             complement(50164..50403)
                     /locus_tag="VQU28_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475567.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPGSNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(50553..50819)
                     /locus_tag="VQU28_000131"
                     /pseudo
     CDS             complement(50553..50819)
                     /locus_tag="VQU28_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(50844..51152)
                     /locus_tag="VQU28_000132"
     CDS             complement(50844..51152)
                     /locus_tag="VQU28_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2475568.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLRLQIAS
                     "
     gene            complement(51077..51319)
                     /locus_tag="VQU28_000133"
     CDS             complement(51077..51319)
                     /locus_tag="VQU28_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HET2475569.1"
                     /translation="MSLADLLEELEAAKDSKKARPMEAYMRHQFSFLGIAVPERNKLY
                     KNIYQKRKKQRLSIGILQTLAGKRSLENTNMWLLTI"
     gene            complement(51320..51952)
                     /locus_tag="VQU28_000134"
     CDS             complement(51320..51952)
                     /locus_tag="VQU28_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2475570.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQEEF"
     gene            complement(51966..52961)
                     /locus_tag="VQU28_000135"
     CDS             complement(51966..52961)
                     /locus_tag="VQU28_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2475571.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLYDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDPDLDASFKS
                     LSGKLNLLTEALQKAENHSLDQEEEIIEKERKRIARDLHDTVSQELFAAHIILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(52958..53656)
                     /locus_tag="VQU28_000136"
     CDS             complement(52958..53656)
                     /locus_tag="VQU28_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HET2475572.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(53734..54744)
                     /locus_tag="VQU28_000137"
     CDS             complement(53734..54744)
                     /locus_tag="VQU28_000137"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HET2475573.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEAKDQMK
                     KA"
     gene            complement(54728..55735)
                     /locus_tag="VQU28_000138"
     CDS             complement(54728..55735)
                     /locus_tag="VQU28_000138"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HET2475574.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLTPNPDYSLIQETIALMGDFLAVRGQNLRSFSLEICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYDVSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKVAAWLEEENLATVLERDWGFSISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKSEKIIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(55722..56675)
                     /gene="mvaD"
                     /locus_tag="VQU28_000139"
     CDS             complement(55722..56675)
                     /gene="mvaD"
                     /locus_tag="VQU28_000139"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HET2475575.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVIADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YSVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(56657..57535)
                     /gene="mvk"
                     /locus_tag="VQU28_000140"
     CDS             complement(56657..57535)
                     /gene="mvk"
                     /locus_tag="VQU28_000140"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HET2475576.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLDITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(57657..57986)
                     /locus_tag="VQU28_000141"
     CDS             complement(57657..57986)
                     /locus_tag="VQU28_000141"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2475577.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMIPHDFPLYLTVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
     gene            58648..58984
                     /locus_tag="VQU28_000142"
                     /pseudo
     CDS             58648..58984
                     /locus_tag="VQU28_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811282.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic-modifying protein"
     gene            58884..59471
                     /locus_tag="VQU28_000143"
     CDS             58884..59471
                     /locus_tag="VQU28_000143"
                     /inference="COORDINATES: protein motif:HMM:NF017000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475578.1"
                     /translation="MTILLKLSSTIVYGEIYHYFLQRDTAKESILDYSFAHGYCEIAY
                     ALFAYSKVLEPSMFYNDLHTFHAELKKLLEKVTSNTENLGNLQLSWCEGISGIILYLC
                     MYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVILA
                     CSERDDHGLLMFQGDSGKADLFDFGIGSRGYIGVY"
     gene            59514..60746
                     /locus_tag="VQU28_000144"
     CDS             59514..60746
                     /locus_tag="VQU28_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2475579.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVHIVANLAGA
                     FLINVISIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFALLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(60864..61820)
                     /gene="cbpC"
                     /locus_tag="VQU28_000145"
     CDS             complement(60864..61820)
                     /gene="cbpC"
                     /locus_tag="VQU28_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HET2475580.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDDLFDNRPVFEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLRSSGAMA
                     TGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGWYQDGSTWYYLNAGN
                     GDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(61918..62607)
                     /locus_tag="VQU28_000146"
     CDS             complement(61918..62607)
                     /locus_tag="VQU28_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2475581.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            complement(62619..64043)
                     /gene="gndA"
                     /locus_tag="VQU28_000147"
     CDS             complement(62619..64043)
                     /gene="gndA"
                     /locus_tag="VQU28_000147"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HET2475582.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            complement(64119..65513)
                     /gene="mapZ"
                     /locus_tag="VQU28_000148"
     CDS             complement(64119..65513)
                     /gene="mapZ"
                     /locus_tag="VQU28_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HET2475583.1"
                     /translation="MSKKRRNRHKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTNSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSEPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            complement(65526..66683)
                     /locus_tag="VQU28_000149"
     CDS             complement(65526..66683)
                     /locus_tag="VQU28_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HET2475584.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARLPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVERKLELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            complement(66768..67148)
                     /gene="rnpB"
                     /locus_tag="VQU28_000150"
     ncRNA           complement(66768..67148)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VQU28_000150"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(67169..67498)
                     /gene="gpsB"
                     /locus_tag="VQU28_000151"
     CDS             complement(67169..67498)
                     /gene="gpsB"
                     /locus_tag="VQU28_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HET2475585.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            complement(67568..68095)
                     /locus_tag="VQU28_000152"
     CDS             complement(67568..68095)
                     /locus_tag="VQU28_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HET2475586.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            68161..68757
                     /gene="recU"
                     /locus_tag="VQU28_000153"
     CDS             68161..68757
                     /gene="recU"
                     /locus_tag="VQU28_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HET2475587.1"
                     /translation="MINYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            68754..70913
                     /locus_tag="VQU28_000154"
     CDS             68754..70913
                     /locus_tag="VQU28_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HET2475588.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGIYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(71080..76383)
                     /locus_tag="VQU28_000155"
     CDS             complement(71080..76383)
                     /locus_tag="VQU28_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HET2475589.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATANENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQQGTVTIREEKGVRYNQLFSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKTPGNSTWYKGNRVVAPEPGSVNRLSITLKSDGQLNASNNDVNLFDTVTLPGAVN
                     DNLKNEKKILLKAGTYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVFLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVNDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGQGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWNGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKPKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLVSDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLASVSLALSALFVVKTKKD"
     gene            complement(76684..78666)
                     /locus_tag="VQU28_000156"
     CDS             complement(76684..78666)
                     /locus_tag="VQU28_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2475590.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHVDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQGSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPVLYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT        22923 a        17948 c        14577 g        23345 t
ORIGIN      
        1 ttctatcttc cattatatca tactttttca gtctatggta gctctcttta aaaggcaccg
       61 atactgccgc cacctccgcc tccagagaag ccaccaccag aacttccact tccagaagat
      121 acagagtagg tgcttgctgt atttgcgaca ctagcataat ggctcatttg cgcgcttgaa
      181 tgataaaaca gactgtgcca gccataagct acatagagat tgatatctgg attttccact
      241 tgaatctgat gaaccttcat caaatgacta accttgtccg catagccaaa taaggtcgca
      301 tagaccaaga ggcgattcca gaccacaata ctttccagtt cagcctgatc caatcgtgca
      361 atctcacgca acatattttc aaaactggtc cagagatagt agacctcagc tcccgcttca
      421 tttagaacac catcacgatt atctagtcga agcttccaat aatagaaaac agacaaaact
      481 aaccctagaa aaccaagtat tggcaaaggg aggtaaagat agccatgaac gtctaaactg
      541 tacaagaaca aaccaaatcc gataaatagg ggcaggatag tcaaggcacc catacccact
      601 tgcaaggcct tttccccacc agttaaagga cgataataat ctgggagccc ccagaaggaa
      661 actcgttttc tcactccttc ttgcatctgg ttcaatacct cttcaaaaga agatttgagt
      721 tgaagccctc ttgcttgaat ccgtttttca tcagaaactt tggctctacg ataaagacta
      781 tcagatacct tgtaatccgc aaacaaattg gaaagagttt cttctttttt acctgaaaaa
      841 gctagattta ggcagtcttt ctcaaagctt gacaaaccat cttcttttac tagcctcaaa
      901 ccaactgcat ctccttctga aatgatagag acattcccac ggtctatcac atctagcaag
      961 gtagcttgaa taagttgatc aaaggtgaat tttccagctc ccttgaccaa gggactcact
     1021 tcctccaagg aggtcgagta gactgcttct gataaaacca taggctctaa ttccattggt
     1081 ggttcataga gacgatgatt tttggcatat ttgactgaag gagtggtctt tcttctataa
     1141 ataaaataga agcagacact caataacaag gagatagaaa ggatcgaagg gaggacccaa
     1201 gtaacaagtt gtttactctg atctttttct ctaacaatcg agtcttctat cttattaaac
     1261 tcttctaaac gattcccttt caatccctga tccctagcgc tagcaaaatc ggtccgaggc
     1321 caataggcat gcaactcaac tccacgctta gccggaagat tgtctaaacg gatagtataa
     1381 tcaaggttac tcttttcaat cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt
     1441 ttttcagctc ccttgtctcc ccttacatga aattcaaact tttcaataga ctctgaacta
     1501 tctgtcagag gttgccaatt taattcagcg atatcatcat aaaggaaaag taaatttttt
     1561 aagttccaga cgaggtcaac ttcaactatg tcgccctcct gacctggatt atagactctc
     1621 acagtataac catccgcttc ttctgttact tcgctagtca catctgctag ttctgcaccg
     1681 tttttcgcgg cctgaacctt tggatgaggg tcaatgtcaa acccgctagg catcttacca
     1741 gcacgtccaa gtcccacgat ttgtccctta aagtcctcct caaactggta aactatcttc
     1801 tgtctaaact ctgccgtatt atctgcatga atatacaaat ccccttgata agagtttatc
     1861 ttgaaatcaa tggcaaaaac agagagtgga agaaggcaaa acaagcctaa caccagtaag
     1921 aaaaaagttt ttttcatcaa ctaagccccc tttttatctt tcttatcatt atagcatttt
     1981 ttcgtaagta aaggcttact tatgttgaaa aattacgcat ttttcgataa aataggagac
     2041 agttattttt taatagatca gaataggaga aatcatgaga aaaatatact tatctatttt
     2101 cacaagtctc ttgctgatgc taggacttgt caatgttgct caagccgatg aatatttacg
     2161 catcgggatg gaagcagcat atgctccctt taactggacc caggatgatg atagcaacgg
     2221 agctgtcaaa atcgatggga ccaatcagta tgccaacgga tacgatgttc aaatcgccaa
     2281 gaaaatcgct aaggacttag gtaaaaaacc tttggttgtt aaaaccaagt gggaaggtct
     2341 agtccctgcc cttacttctg gtaagattga catgattatc gcaggtatga gtccaactgc
     2401 agaacgcaaa caagaaattg ccttttcgag cagttactac actagcgaac cagttttgct
     2461 tgtcaaaaaa gattctgcct acgcaagtgc taaatctttg gatgacttta acggtgcaaa
     2521 aatcacttct caacaagggg tctacctcta cgacttgatt gcacaaatcc caggtgctaa
     2581 aaaagaaaca gccatgggag acttcgctca aatgcgacaa gctcttgagg ctggtgtcat
     2641 tgatgcttat gtttctgaac gtccagaagc actgactgct gaagctgcga actctaagtt
     2701 caagatgatt caagtagaac ctggtttcaa aactggggaa gaagatacag ctatcgctat
     2761 cgggcttcgt aaaaatgaca atcgtattag ccaaatcaat gccagcattg aaaccatttc
     2821 aaaagatgac caagttgcct tgatggatcg tatgatcaag gaacaacctg ccgaagctac
     2881 aacaactgaa gagactagca gtagtttctt tagccaagtc gctaaaattc tttctgaaaa
     2941 ctggcaacaa ctcttgcgtg gtgctggtat cactctttta atctctatcg tcggaaccat
     3001 cataggtctc attattggac ttgccattgg tgtcttccgt actgctcctc tctctgaaaa
     3061 caaagccatt tatggccttc aaaaactagt cggctgggtt ctcaatgtct acattgaaat
     3121 tttccgtggt acgccaatga ttgttcaatc gatggttatc tactatggaa ctgcccaagc
     3181 tttcggtatc aaccttgacc gtacactagc tgctatcttc atcgtttcaa tcaataccgg
     3241 tgcctacatg actgaaattg tccgtggtgg tatcctggca gttgacaagg gacaatttga
     3301 agctgcgact gctcttggta tgactcataa ccaaaccatg tgtaagattg tcctacctca
     3361 ggttgtccgc aatatcctac cagcaactgg taatgaattt gtcatcaata tcaaagatac
     3421 atctgtattg aacgttatct ctgttgtcga actttatttc tcaggaaata ccgtggcaac
     3481 acaaacctat caatacttcc agacatttac aatcatcgcc gtgatttact ttgtcctcac
     3541 cttcaccgta acacgtatcc tacgctttat cgagcgcaga atggacatgg atacctacac
     3601 tacaggtgct aaccaaatgc aaacggagga tttgaaataa tgacacaagc aatccttgaa
     3661 attaaacacc tcaaaaaatc ctatggacaa aacgaagtgc taaaagacat ttctctcact
     3721 gtccacaagg gagaagttat ttctatcatc ggaagctctg gaagtgggaa atcaaccttc
     3781 ctacgctcca ttaacctact tgaaacgcca actgatggac aaatccttta tcatggacaa
     3841 aacgtcctcg aaaaaggcta tgacctcacg caataccgtg aaaagttagg gatggttttc
     3901 caatccttta acctctttga aaatctcaac gttcttgaaa acacaatcgt cgctcagaca
     3961 actgtcctta aacgcgaacg cacagaagct gaagaaattg ccaaagaaaa cctggaaaag
     4021 gtcggcatgg gagaacgcta ctggcaagcg agacccaaac aactctcagg tggtcaaaaa
     4081 caacgtgtgg ccatcgctcg tgccctttca atgaatcctg acgccattct ctttgatgaa
     4141 ccaacatcag ctctcgatcc agaaatggtt ggagaggtcc tcaaaatcat gcaagatctg
     4201 gctcaggaag gcttgaccat gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc
     4261 tctcaccgtg ttatctttat ggataagggt gtgattgctg aagaaggtaa accagaagac
     4321 ctcttcacta atcctaaaga agaccgtaca aaagaatttc ttcaacgtta tctcatactc
     4381 aatgaaaatc aaagagcaaa ctaggcagct agccgcaggt tgctcaaaac actgttttga
     4441 ggttgcagat agagctgacg tggtttgaag agattttcga agagtatcaa ataaaaagaa
     4501 aaggctgcat caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc agattctcta
     4561 tctctactac ttgaaggtca agaatccacc gttatccaaa gattaatctt caaatttttc
     4621 atgatttttt tcatagaaat caattaagcc tagggctgtt tcaaatgaaa tattttttac
     4681 ttttgcacga ccctgcgcca gagcaatgat agacatttca cgcgcattcg tttctttaga
     4741 gatacggtag cctgtgattt tcttatcacg aacccagcca acaactgatt ctactttttc
     4801 aaagtttgac ttagccatgt ttttctccta ttttcttctt tataatattt aattgtatac
     4861 gtttttacta cttttgtcaa taaaaaaaac atatattcaa cgaaaataat tagttcagaa
     4921 ttttcttact tcctttttaa atccgataat atataggttt tgctagcctc tacaccataa
     4981 atatttgcta ataaaaatac tgtattatta ccctcttaag gtgcaaaatt attcaagttc
     5041 ttaaaaaatg cttctctatt tccgtaagat agcttatttc ttcgatttaa gaactgaaga
     5101 tgatagttct aacctttaga aatccaagcc ttctctagct caaaaactcc tatctttctt
     5161 cctcaataaa tcatctgaga gataggtatt ccttatatcc ttcaaattca tcactccttt
     5221 ggatattgag gtgctagcct agaagcagac acccgattat ctaaaccatc cttaatagag
     5281 atgacaagac agataagctc agttaattac ttatatatct ttttaatatg gtatatttgt
     5341 caacaaaaag aaatcaaatc ataccttata ctcaatgaaa atcaaagagc aaactaggaa
     5401 gctagccgca aattgctcaa aacactgttt tgaggtttca gatagagcta acgtggtttg
     5461 aagagatttt cgaagagtat taaagacagt atgactatgc ttgtcaatag ataggaaatc
     5521 aaaatgaagg atggaaatct gagccaaaca tcataagtgc tataatgaac agaagtaaga
     5581 attgcacgat aagttttcta aaaccatagg aatcgtcccc tttcttcatc ttcattatag
     5641 cacatataca tcagttgtac aaataatatg atattttttt attagtcctc agacaagcat
     5701 attataaagc gtttcattac catttaagtt aatataagct ggatcaaaac cttccattct
     5761 acggatcaaa cctgcataat catgcttatc tgccaaagcg atcccaatca atactgggcc
     5821 tgttcccttg ctagctcgtt tgatatactc aaaacgtgtg atatcatcat ttggccccag
     5881 gatatcattt acaaactcac gcaaagctcc tggacgctgt gggaaattga ccacaaagta
     5941 atgtttgata ccatcataaa tcaaggcacg ctcttccatt tctggcatac ggttgatatc
     6001 attatttcct ccagaaatga tacaacaaat ggttttcccc ttaatatatt cagctaaaac
     6061 ctctaaagag gcgatactag ccgctccagc aggttctgcg actatccctt gcttagagta
     6121 aaggtcaatc aaggtttcag aaatcaatcc ctcatcgaca cctaccaaag tttgaatatg
     6181 ttgacgagtt gcttcatagg tcaactgacc taccttttgc acagcaatcc catccgcaaa
     6241 tttatcaatt tccttgagtt tgactggacc tccagcctca aaggcagctt tcatggaacg
     6301 cgctccattc gcctctactc cgataacctc aatctctgga cttgtttcct tgatataggt
     6361 agaaaccccg gcaatgagac cgccaccacc aacaggaacc aagacagcat caaaatcaat
     6421 cgattctttt cgagcttctt ctaaaatctc ataagcaact gttccttgac ctgcttgaac
     6481 atgagcatca tcaaaaggat caataaaggt acgattttca gagactgtaa attcttgagc
     6541 tgctttggct gaggcatcaa aggtatctcc aactagttta atagttacaa aatccccacc
     6601 aaaaaagcga acctgaccaa tcttttgttg tggcgtagta ataggcataa agatagtagc
     6661 aggaattttc atttcattac aagtataggc tactccctgc gcatgatttc ccgcagaagc
     6721 gcagactacc ccacgttcac gttcttcctt gctgagctgg gaaatggcat aataggcacc
     6781 acgaatttta aaggagcgaa cacgctgggc attttctttt ttcaaataaa tcttagcacc
     6841 atacttctcc gataaataat gatcgtaatc cagtggagta ttcacaacca caccgttcaa
     6901 gaccttgtga gccttgatta tatcttttga acttaacata taaaacctct ttttctataa
     6961 aaaacagtca ctaatgactg ttttttagtt attcatctcc gaaaacttca gtaacacttt
     7021 catggtattt atgagccata cgaatcgaaa actctttagg aagttcataa cgatttgtgg
     7081 aaatccaatc atataaccat tctaccaaac tttcttcact atcattacga tggaagataa
     7141 aatagtttaa aatttcttta tattctacat cattcaaatc caacgtgtct acaaattgac
     7201 tacgaggaac aaaattagaa tctaattttc ttagttgatc tgtcaattct cccaattgta
     7261 aatccaactg aaaaaatagt ttttgcagtt gcctatcagt ttcatcacat cctgtcttga
     7321 tagaaaacaa attggtaata ttcatatgat ttcaacccct aaataagtac atctaatatc
     7381 ccacgaatag ctgaagctaa agttttcgca gtccagtcat ttaaacccga ttctttcaat
     7441 ccagatgcaa taatttgctc tatttgatat aaaccgccat ctatcgtctc atttattctg
     7501 cgaagaggac cttcaatata ggataaagct tctccgaatc taacagctga tttaggtcct
     7561 aatgatttac caactatatt tgttaatgtt ttttatttct caaaatccat ttcgctatat
     7621 aacgagcaga tttagtcgca ccagctctct caaacggaga atgaatttca agaccttctt
     7681 ctctagctat ttgtgccaat ttgaagaatt gttcaaaggt catatgcaac ctaccatctt
     7741 caaaattagc atcatcataa atgttaatta cagcattttc atcatacgac caatctgctt
     7801 tattttggtc tgctagcact attaccgacg gtgtaattac attatacaac tagaaccaat
     7861 aataacgatg caatttttct cataatcttt ttcataaaat catctccagc gctatatatt
     7921 tctaattagt tatagatttt gaatgcatca tcgtcgtttt taccaacaaa tggcattgct
     7981 ttacgcaatt ctgcaccaac tttttcaatt tcaaggttag ctgcttgttc acggtaagca
     8041 gtcaattttg gacgtccagc tttatagtca tttacaaagt catttgcaaa tttaccattt
     8101 tggatgtctg ccaagacagc cttcatattt tctttaactt gttcagtgat tacacgtgga
     8161 cctgatacat agtcaccgta ttcagcagtg tttgaaatag attgacgcat tttcttgaat
     8221 ccaccttcgt agatcaagtc aacgatcaat ttcatttcgt gaagaacttc aaagtaagcc
     8281 aattctggag cgtaacctgc ttctgtcaag acttcgaaac ctgcttcgat aagggcagtc
     8341 aaaccaccac aaagtacagc ttgttcacca aacaaatctt cttcagtttc ttctttgtaa
     8401 gttgtttcaa gaagacctac acgagccgct ccaacacctt tacaccagtc catagcaatg
     8461 tttttagcat ttcctgttgc atcttggtat actgcataaa gagctggaac accaaatcct
     8521 tcttcgtaag tacgacgtac caagtgtccc ggtcctttag gagcacacat gaagacatct
     8581 acatccgcag gaactttgat aaattcaaag tggatgttga aaccatgggc aaatccaact
     8641 gcgtttccag cttccaagtt tggagcgatt tctgcttcgt acaattcttg ttgaatttcg
     8701 tctggcgcca agatcatgat aacatcagcc aacttagtag cttctactac tgtgtaagta
     8761 tcaaatccat cttcttttgc tttatcaaaa gatttacctg gacgtacacc gataataacg
     8821 tcacgacctg aatcacgcaa gttttgagca tgcgcatgtc cttgtgaacc ataaccgata
     8881 acggcgattt ttttaccgtc aagtgctgct actttaacat ctttttcata ttccatttga
     8941 actgtcatag cttttctctc ttttctatta tttattgcct tttaggctgg tttaataaat
     9001 ttaagttgga tttttaatcg cgggtaaatc cagttgcacc cgttcgagca atattgcgaa
     9061 taccgtatgg gcgaatgact cgcaataggg cttcgctctt ttctgcattt cccgtcatct
     9121 gaatggtaat cgagcttggc gctacgtcta ccactgttgc acggaaaggt tgaataatcg
     9181 ctaaaatctc agctctcttc tcagctggcg ctgacatctt aaccaaaatc acctcgcgct
     9241 ccaaatgagg cttgtctgta atatctcgaa tgcgaatcac atcaatctga cgattgagct
     9301 gtttgatgat ttgctccact tcatcatgag aagcaacatc aataataata gtgatacgcg
     9361 atacattcgg atcttctgtt gctccaacag agatgctttc aatattaacc tgacgacgag
     9421 ataggacacc tgtaaagcga ttgaggactc ctgaacgatt ttgtagtttt gctgttaaca
     9481 ttctacgcat ggaactgcac ccccaacatc tcatgattac tcttaccagc cggtaccatt
     9541 ggtaacacct gttccttacg agaaatatct acctcaatta gcataggaac atcctcagtg
     9601 atgacttcaa ggtcttgagc caaggtctca ggattgtcaa acttatagtt tttaatacca
     9661 taagcctgcg ccatcaattg gaaatcagga agggtatcaa agaccgactc tgatgttctg
     9721 ccttcataga aggattcctg ccactggcga accattccaa gtgaatgatt gttcagcata
     9781 accaccttga ttggcacctt gtaaatattc aaaatagcca actcctggtt ggtcatttgg
     9841 aaaccaccat ccccaacaaa caagactact tccttatctg ggttagcaat tttagcaccg
     9901 attgctgctg gaattccaaa gcccattgtt cccaaaccac ctgaagtcac taactgacgc
     9961 tcattttggt agggataata ctgagctgtc cacatttggt gttgaccaac gtctgttacc
    10021 acaatggcat ctccattcgt caattcacca attcgttcaa taactgcttg cggttgaacc
    10081 acacgctctt tcttgtcata agaacgaaca cgattcttgt ctttagtgac tttctcaatc
    10141 cacttttcag tattgttgtg aactgttggt tctgctagca acatttgcaa ggccttctta
    10201 gcatctccaa ctacaggaat gtctgcactg ataatcttgc caatctcagc tgggtcaata
    10261 tcaatgtggg caaccttagc attcttagcg aaagtcttag gattccccgt caaacggtca
    10321 tcgaaacgag aaccaatact aatcataaag tccgcttccg tcatggcaat atttgctgcg
    10381 aatgacccgt gcatgcctcc cattccaaga aagagtgggt gactcgttgc aatcgttcct
    10441 tgtcccaaaa gactggttac cactggaatt tgatagcgtt ctgcaaattc atttagttcc
    10501 gtagcagcct cagcataact aattccacca ccagctaaca agactggctt tttagccttg
    10561 gacaattgct tcaagatttt cttgatttgc atatcattcg gctcaagagt cggctgataa
    10621 cttggtaaat tcacttctgg tgaataaatg aagtctgttt ctaaagcaga tatgtctttt
    10681 ggtaggtcaa ttacaactgg ccctggacgg cctgtagttg cgatatggac agcttccgta
    10741 atgatacgcg gaatatcagc tgtctcacga acttggtaat tgtacttagt gattggcatg
    10801 gtaattccta cgatgtctgc ctcctgaaag gcatccttcc caatccctgc tcgcgccacc
    10861 tgacctgtaa agaccaaaag gggaacgcta tcgctcatag catccgcaat ccctgtaatg
    10921 gcatttgttg ctcctggtcc actagtgacg acggcaacac ccaactttcc agttgatttg
    10981 gcataacctt cagcttcatg caaacaacct tgctcatggc gccctagaat gtggcgaatg
    11041 cctttaaaat tatatatcgc atcataaaaa ggcaaaaccg caccaccagg ataaccaaag
    11101 atggtatcaa ctcctaaatc acgaagtgtt tccaaaacta ggtccgaccc cgtcttagga
    11161 gattctaaac tgattttctc cattgttccc ctttctttcc tcttaaaaat aacttgttac
    11221 tatcatacca ttttttcaaa atttttcaag ataaaagaag aaattttctg aattttctat
    11281 tttaacgttt atttatgaat gttatttttg tttttattaa aaagataccg atttcattta
    11341 ctaaaaaaga aaaaagaact gatttctcag tccttcatta atcttattcc acactaaata
    11401 ggtatgggta aacaggttgt tgaccttggt gaatctcgac ttcaacgtct tcgaattctt
    11461 ctacgatttc ttgagcgatt tcattggcaa gttcttcgct tccgtcttca ccgacataga
    11521 aggttacgat ttcactatct tcatccaaca tatgtttcaa ggtttcagtc aatgtttggt
    11581 gcatatcagg gtttgacaca agaatttttc catccaccat acctagatta tcgttttcat
    11641 ggatttctaa gccatcgatc gttgtatcac gcacggctgt tgtgacgctt ccgctaacga
    11701 catcgctaag agcagctgtc atacgctctt ggttttcttc aatggacttg cttggatcaa
    11761 aggcaagaag acttgtcata ccttgaggaa gagtgcgagc ctctaccact actgctggtt
    11821 gctccaaaac ttctgccgca gattgagctg ccatgaagat gttcttgttg tttggcaaga
    11881 agatgatgtt acgggcattg acctgttcaa cagccttgat aaagtcttct gttgaagggt
    11941 tcatggtttg accgccttcg ataacataat ccacgccttg agaacagaag atatctgcta
    12001 gacctttacc agccaccaca gcaatcaaag catactcttt ttcttcagcc gacttgataa
    12061 cttgagtagc ttctttctca acctgtgctt cgtgttggtt acgcatattg tcaactttta
    12121 ccttgaccaa gctaccatat ttgagacctt cttgcataac aagtcctgga tcttctgtat
    12181 gaacatggac tttgacaatt tcatcatcgt taacaacgag gagagaatcc ccaagctcat
    12241 ccaagtagtt acggaattca tcgtagtcaa aatctttagc ataggttgga ccttgcttaa
    12301 gagctaccat gatttcagta cagtaaccaa acgtgatgtc ctcagtcgct acgtgaccag
    12361 ctacagactt atgatgctct acattgatca tctcactcat gttggcagga gtcgctacaa
    12421 agtcctcaga tgcaatatat tcgccagtaa gggctgaaag gaaaccttcg tagatgaaga
    12481 ccaatccttg accacctgag tccacaacgc caacttcttt caatactgga agcatgtctg
    12541 gtgttttagc tagagctgtt ttagcacctt ccaaggctgc gcgcatgact tcaacagtgt
    12601 catctgtttg ctcagctttt ttcttagcac cgatagcagc tccacgagaa actgtcaaaa
    12661 tcgttccttc aacaggtttc atcactgcct tataggcaac ttccacacct gattggaagg
    12721 ccagagccaa gtcttgacct gttaactcgt ctttatcctt gatagcttgg gaaaatccac
    12781 ggaaaagctg agacgtaatc actcctgagt tcccacgcgc acccatcaaa agccctttgg
    12841 caagaatgct cgctacttct ccaactgtag aagctggctt gtctgcaact tctttagcac
    12901 cattttcaat ggtcattccc atatttgtcc cagtatctcc atctggaact ggaaagacgt
    12961 ttaatgaatt gacatattca gcttgcttat tcaagcgagt tgatgcagcc tgcaccattt
    13021 cttgaaataa gctagtagta atttttgaca cggttattct cctacaactt tgatattttg
    13081 aatgtagaca tttacagtct gagcagtaat tccaagctgg ttttccaagc taaaggcaac
    13141 acgctcttga atgttttttg acacttcact aatctttgtt ccgtagctta acacggtata
    13201 tacatcaact gcaatactgc catcttcggc tgcctttacg acgacacctt tagaataatt
    13261 ttccttacct agcagggctt ggaaattatc tttgagggca tttttactag ccataccgac
    13321 cacaccaaaa atctcagttg ctgctccacc tacgacggtt gcaatcactt catctgttag
    13381 ttcgatttga ccatcttttg tattaatttt tacagtcatc ctttttacct caactagttg
    13441 atactctatt ttatcatatt tcagcccaag tgtaaaagca gaatgatgta tcagcggata
    13501 tttactctat ttttcaaacg atgttatacc cacaataaaa gaaaaaagac cctaaggtct
    13561 cctttgcttt tattattaaa cgcgttcaac tttacctgat ttcaaagcac gagctgaagc
    13621 ccaaactttt ttaggtttac catcgataag aacagtaact ttttgaaggt ttggttttac
    13681 ggcacgtttt gtttggttca tcgcgtgtga acggttgttt cctgatacag tcttacgacc
    13741 tgtaaagtaa catactttag ccattgtgtt ttcctcctat tagatctaat atagcggatg
    13801 tgctagcacc acataccgta ctatgttatc acactttctt gttttttgca agggaattgg
    13861 aagatttttt atttgtgtct taaatcaggt cttgcgtgac attttctgct ctccacatgc
    13921 catcgttgat taacagaaca ccagaattaa aattatgtgt ataaaaatca tctctaactg
    13981 cagctaaggg tatagccgtc aagtccaaat cccacagctc atctatcgat tttcttacaa
    14041 caatatctga atccaaatac agtacacgag actcgcttac atactttgga ataaaatacc
    14101 taaaaaagcc gcatatgaaa gtccctcaaa ggggagacga taacctttca gaatattact
    14161 gtcaatctaa acattcacaa tctcactatt caaagtctct agtctttttt ccatcaattg
    14221 gaaccattct cgcggaaggt catcattaaa aacataaaac ttaagattat aatgatgaac
    14281 acaaagagat tttattgttg tttcaacttt atccatataa gcattatctg cacctaagac
    14341 aatcgctttt ttctcttttt tctcttcttt cactttttat ctcatttctt tttattccca
    14401 tcatatgttt ctacgtaacc attattttcg cctattcgtt cgtaaaacca taccagtgga
    14461 gattttagat gaagtcccat tacggtttac aatttttaca ttacgacacg gagttttaca
    14521 aatcgatttc atttgccaaa cgtagttagt gaggcagtta gctagttcgc caaatagcga
    14581 ctagcgtcca acaatttgga actttagttc caattgttgg tactgagtca catcttctcc
    14641 tctaactcta cgtctggata cttgtcagca aaccagcgga gggcaaagtc attttcaaag
    14701 agaaagactg gttggtcaaa acggtctttg gctaagatat tgcgacttga cgacatccgt
    14761 tcatccaagt cctcaggctt gatccaacga acggtctttt tacccattgg gttcataact
    14821 acttctgcat tgtactcgcc ttccatgcgg tgtttaaaga cttcaaactg gagttgacct
    14881 acagcgccta gcatgtactc acctgtttgg taattcttat aaagctgaac ggctccttct
    14941 tgcaccaatt gctcaatccc cttgtggaag gatttttgct tcataacatt cttagcagaa
    15001 actttcatga aaatttcagg agtaaaggtt ggcagtggtt caaattcaaa cttgttttta
    15061 ccaactgtca aggtatcccc aacttgataa gtaccggtat cgtaaacccc gataatatca
    15121 cctgccacgg cattgatcac attctcacga ctttccgcca taaactgggt aacattagat
    15181 agtttggccc ccttaccagt acgagggaga ttgacactca tgccacgctc aaattcgcct
    15241 gatacgatac ggacaaaggc aatacggtca cggtgacgag gatccatgtt ggcttggatt
    15301 ttaaagacaa agcctgagaa atccttgtca taaggatcca caatttcacc gtctgttttc
    15361 ttgtgaccat gtggttctgg agcaaacttg aggaagattt caaggaaggt ctgcacacca
    15421 aagtttgtca gggctgaacc gaaaaagaca ggcgtcaatt ctccagccag aatagcttcc
    15481 tctgaaaact cattcccagc ttcatttaaa agctcaatgt catccttgac ttgctcgtag
    15541 aaaggattgc taccaaagag tttgtccccg tcttctagac tggcaaaacg ctcatcccct
    15601 ttgtaaagct ctaaacgttg gttatagagg tcatacaagc cctcaaaggc tttccccatc
    15661 ccgataggcc agttcatagg gtagctagca atgcccaaga tttcttccaa ttcttgcaag
    15721 agatccaaag gctcacgacc gtcacggtcc agcttgttca taaaggtaaa aactggaatg
    15781 ccacgatgtt tcacaacctc aaacaatttc ttggtttgag cctcgatccc cttggcagag
    15841 tccacgacca tgaccgcagc atccaccgcc atcaaggtac gataggtatc ttctgagaag
    15901 tcctcgtgcc ctggcgtgtc taagatattc acgcgcttgc cgtcgtagtc aaattgcata
    15961 acagatgaag taacagaaat cccacgttgc ttctcgatat ccatccagtc agatttagca
    16021 aaagtccctg ttttcttccc ttttaccgta ccagcctcac gaatctcacc cccaaagtag
    16081 agtaactgct cagtgatggt tgttttcccc gcgtccgggt gggagataat ggcaaaggta
    16141 cgacgtttct taatttcttc ttgaatattc ataagttctc tttctttgat tctctatttt
    16201 tcttgtttca atagctgaga atgattttta cattggattt taccattcct ttcaacattc
    16261 cattatatcg gattttagca tttttttcaa tttctatttc ttttcacttc cccctccctt
    16321 atttatagga aaatatggta aaatagaaca gactaaaaat catcatttca cgaaaggatg
    16381 caagatgaaa attacgcaag aagaggtaac acacgttgcc aatctttcaa aattaagatt
    16441 ctctgaagaa gaaactgctg cctttgcgac caccttgtct aagattgttg acatggttga
    16501 attgctgggc gaagttgaca caactggtgt cgcacctact acgactatgg ctgaccgcaa
    16561 gactgtactc cgccctgatg tggccgaaga aggaacagac cgtgatcgct tgtttaaaaa
    16621 cgtacctgaa caagacaact actatatcaa ggtgccagct atcctagacg atggaggaga
    16681 tgcctaatga cttttaacaa taaaactatt gaagagttgc acaatctcct tgtctctaag
    16741 gaaatttctg caacagaatt gacccaagca acacttgaaa atatcaagtc tcgtgaggaa
    16801 gccctcaatt catttgtcac catcgctgag gagcaagctc ttgttcaagc taaagccatt
    16861 gatgaagctg gaattgatgc taacaatgtc ctttcaggaa ttccacttgc tgttaaggat
    16921 aacatctcta cagacggtat tctcacaact gctgcctcaa aaatgctcta caactatgag
    16981 ccaatctttg atgcgacagc tgttgccaat gcaaaaacca aaggcatgat tgtcgttgga
    17041 aagaccaaca tggacgaatt tgctatgggt ggttccggtg aaacttcaca ctacggagca
    17101 actaaaaacg cttgggacca cagcaaggtt cctggtgggt catcaagtgg ttctgccgca
    17161 gctgtagcct caggacaagt tcgcttgtca cttggttctg atactggtgg ttccatccgc
    17221 caacctgctg ccttcaacgg aatcgttggt ctcaaaccaa cctacggaac agtttcacgt
    17281 ttcggtctca ttgcctttgg tagctcatta gaccagattg gaccttttgc tcctactgtt
    17341 aaggaaaatg ccctcttgct caacgctatt gccagcgaag atactaaaga ctctacttct
    17401 gctcctgtcc gcatcgccga ctttacttca aaaatcggcc aagacattaa gggcatgaaa
    17461 attgctttgc ctaaggaata cctaggcgaa ggaattgatc cagaggttaa ggaaacaatc
    17521 ttaaacgcgg ccaaacactt tgaaaaattg ggtgctatcg tcgaagaagt cagccttcct
    17581 cactctaaat acggtgttgc cgtttattac atcatcgctt catcagaagc ttcatcaaac
    17641 ttgcaacgtt ttgacggtat ccgttacggc taccgcgcag aagatgcgac taatcttgat
    17701 gaaatctatg taaacagccg tagccaaggt tttggtgaag aagtgaagcg ccgtatcatg
    17761 ctgggtactt tcagtctttc atcaggttac tacgatgcct actacaagaa ggctggacag
    17821 gtccgtacac ttatcattca agatttcgaa aaagtcttcg cggattacga tttgattttg
    17881 ggtccaactg ctccaagtgt tgcctatgac ttggattctc tcaaccatga cccagttgcc
    17941 atgtacttag ccgacctctt gaccatacct gtaaacttgg caggactgcc tggaatttcg
    18001 attcctgctg gattctctca aggtctacct gtcggactcc aattgattgg tcccaagtac
    18061 tctgaggaaa ccatttacca agctgctgct gcttttgaag caacaacaga ctaccacaaa
    18121 caacaacccg tgatttttgg aggtgacaac taatgaactt tgaaacagtc atcggacttg
    18181 aagtccacgt agagctcaac accaattcaa aaatcttctc acctacttct gcccactttg
    18241 gaaatgacca aaatgccaac actaacgtga ttgactggtc tttcccagga gttctaccag
    18301 ttctcaataa aggggttgtt gatgccggta tcaaggctgc tcttgccctc aacatggacc
    18361 tccacaaaaa aatgcacttt gaccgcaaga actacttcta ccctgataat cccaaagcct
    18421 accaaatttc tcagtttgat gagccaatcg gttataacgg ctggattgaa gtcgagctag
    18481 aagacggtac gaccaagaaa attggtatcg aacgcgccca cttagaggaa gacgctggta
    18541 aaaacaccca cggtacagac ggctactctt acgttgacct taaccgccaa ggggtacctt
    18601 tgattgagat tgtatctgaa gccgatatgc gttcgccaga ggaggcctat gcttatctga
    18661 cagccctcaa ggaagttatc cagtacgctg gcatttctga cgttaagatg gaagaaggtt
    18721 ctatgcgtgt ggatgccaac atctcccttc gtccttatgg tcaagagaaa ttcggtacca
    18781 agactgaatt gaagaacctc aactccttct caaacgttcg taaaggtctt gaatacgaag
    18841 tccaacgcca ggctgaaatt cttcgctcag gtggtcaaat ccgccaagaa acacgccgtt
    18901 acgatgaagc gaataaagca accatcctca tgcgtgtcaa ggaaggggct gctgactacc
    18961 gctacttccc agaaccagac ctacccctct ttgaaatttc tgacgagtgg attgaggaaa
    19021 tgcggactga gttgccagag tttccaaaag aacgtcgtgc gcgttatgta tctgaccttg
    19081 gtttatcaga ctacgatgct agtcagttga ctgctaataa agtcacttct gacttctttg
    19141 aaaaagctgt tgccctaggt ggtgatgcca aacaagtctc taactggctc caaggggaag
    19201 tcgctcagtt cttgaatgct gaaggtaaaa cactggaaca aatcgaattg acaccagaaa
    19261 acttggttga aatgattgcc atcatcgaag acggtactat ttcatctaag attgccaaga
    19321 aagtctttgt ccatctagct aaaaatggcg gtggcgcgcg tgaatacgtg gaaaaagcag
    19381 gtatggttca aatttcagat ccagctatct tgatcccaat catccaccaa gtctttgccg
    19441 ataacgaagc tgctgttgcc gacttcaagt caggcaaacg taacgccgac aaagccttta
    19501 caggattcct tatgaaggca accaaaggcc aagccaaccc acaagttgcc cttaaactac
    19561 ttgcacagga attggcgaag ttgaaagaaa actagacaga acaaaaccag ccctaaggtt
    19621 ggttttttct tctctaccaa ctcccaataa ctattttggc tttatttcca gagtatttta
    19681 tggtaaaatg aagagtaata atatttatta aagaggtaaa aacatgattg aagcaagtaa
    19741 attaaaagct ggtatgacct ttgaaacagc tgacggcaaa ttgattcgcg ttttggaagc
    19801 tagtcaccac aagccaggta aaggaaacac gatcatgcgt atgaaattgc gtgatgtccg
    19861 tactggttct acatttgaca caagctaccg tccagaggaa aaatttgaac aagctattat
    19921 cgagactgtc ccagctcaat acttgtacaa aatggatgac acagcatact tcatgaatac
    19981 agaaacttac gaccagtacg aaatccctgt agtcaatgtt gaaaacgaat tgctttacat
    20041 ccttgaaaac tctgatgtga aaatccaatt ctacggaact gaagtgatcg gtgtcaccgt
    20101 tcctactact gttgagttga cagttgctga aactcaacca tctatcaaag gtgctactgt
    20161 tacaggttct ggtaaaccag caacgatgga aactggactt gtcgtaaacg ttccagactt
    20221 catcgaagca ggacaaaaac tcgttatcaa cactgcagaa ggaacttacg tttctcgtgc
    20281 ctaatctcta gaaagaggtc attctatggg aattgaagaa caacttggcg aaatcgttat
    20341 cgccccacgt gtacttgaaa aaatcattgc tatcgctact gcaaaggtag agggtgttca
    20401 ctctttttca aacagatcag tgtctgatac cctttcaaaa ctttcactcg gccgtggcgt
    20461 ttatcttaaa aacgtggacg aagaactcac agcagatatc tatctctacc ttgagtacgg
    20521 agtaaaagtt cctaaggtag cggttgctat ccagaaagct gtcaaagatg ccgtccgtaa
    20581 tatggctgat gtagaactcg ctgctatcaa tattcacgtt gcaggtatcg tcccagataa
    20641 aacaccaaaa ccagaattga aagatctatt tgacgaggac ttcctcaatg actagtccac
    20701 tattagaatc tagacgccaa ctccgtaaat gcgcttttca agctctcatg agccttgagt
    20761 tcggtacgaa tgtcgaaact gcttgtcgtt tcgcctatac tcatgatcgt gaagatacgg
    20821 atgtacaact tccagccttt ttgatagacc tcgtttctgg tgttcaagct aaaaaggaag
    20881 aactagataa gcaaatcact cagcatttaa aagtaggttg gaccattgaa cgcttaacgc
    20941 tcgtggagag aaacctcctt cgcttgggag tttttgaaat cacttcattt gacactcctc
    21001 agctggttgc tgttaatgaa gctatcgagc ttgcaaagga cttctccgat caaaaatctg
    21061 cccgttttat caatggactg ctcagccagt ttgtaacaga agaacaataa aaagaaaaag
    21121 tgtttgatag atatcaaaca ctttttcttt gcctcccact atctaaaaaa atgataatag
    21181 atataattgt aaacaaaaat ccagataggt tttgcatgat tgagaaagtt aaaaaaacta
    21241 tggcagagaa tcgttaatct cagattgtcg gtagaacgat aaacaagggc aaaaaagaaa
    21301 ccaatcagac tataatataa taaactaatt ggatctctgt gagatagtat caaatggcta
    21361 atcccaaaga taaggcctga taagatgaca tctaaatagt actttgactt aggaaagaag
    21421 gtattcataa aataccctct atcaagagtc tcctcaaaaa caggaccgat gattacaagc
    21481 aggacaaaag ataagatagt cgataaaaag gttggttgtc catttgaaag aaccacggta
    21541 aaatactcat catgaatatt cctatggtta atcaaatgag catagcgtgc ctaaaaatta
    21601 ccgagaatct gataaaccac ataagttgca aataaggaga agacaaatga ccagtcccag
    21661 ctctttttct tttttaacct ccaaattaat agaaaaggaa acttcccact aatcccactg
    21721 ttaaaataag agaatagaca tcagctccta accctaaaat gatcgtcaca tacaatccaa
    21781 ttgtttgtgg taaataggta gatagtaaaa taataagcaa aaatattcca aattgtctta
    21841 gtttctttgt gtttctcatc gtactttttt gaaagattac cctgttcgga agccgtactt
    21901 ccaagcatct atataagaat taagtgcccc ttgcctcata tagggagcaa attctctata
    21961 atataaccat ctactatatc catcttccca aacagcaaga ccacctgaag tttgctccaa
    22021 gtcctcagtt gaaagaactg taaatgtttc tgtacctgtc attgcaagta ccttcttaaa
    22081 atagattgtt gtaggctcac atttatagta tatttctttt tttgtctatt ttatagtcta
    22141 tctcctcaac tggcaatttt tcgacctgaa ttgcattttt ccataaaaaa tgagaccttt
    22201 ctagtctcat ttagtcattc ttagtatttt ctaaatcgtt gatagcgttc ttccagcaac
    22261 tcttctagcg gtttttgtga aagtctagcc agctccgttt ggagttcttt tttgacactc
    22321 ttaattagtt ctttactaga aagtcctact tcagaaatca ccttatccac cacgtccatt
    22381 tctaacagtt catgcgaagt gattttcatc agttctgctg cttccatggc gcgagtaccg
    22441 tccttccata aaatggaagc aaagccttct ggactgagaa tggcatagat agaattttcc
    22501 agcatccaga cacggtccgc gacagctaga gccagagccc cgcctgaacc accttcaccg
    22561 ataataatgg cgataatagg aactttcagg tcactcattt ccatgagatt gcgagcgata
    22621 gcttcccctt gaccacgttc ttccgctccg acaccaggat aagcacctgc tgtattgata
    22681 aaggtcacaa ctggacggcc aaatttctca gcctgtttca tcaaccgcag tgcctttcgg
    22741 tagccttctg gatgtggttg gccaaaattc cgtttgaggt tgtcttgcaa actcttgcct
    22801 ttttggatac caaccactgt tacagcttgg tctccaagcc aaccaatacc accaacaact
    22861 gcaccatcat cacgaaaaga acggtcacca tgtaattgga taaattcatc aaaaatgcct
    22921 gtcgcaaagt ccaaggttgt caagcgactc tgctcacgcg cttctctgac tatttttgca
    22981 atattcatct aggactccct ccatgcaatc tgactaggct agcaatcgta tctggtaagt
    23041 ctcttctttt gacaatagca tccacaaagc catgttctaa taggaattct gccttttgga
    23101 aatcctcagg caagctttca cgaaccgtat tttcaatcac acgacgccca gcaaaaccaa
    23161 ccaagctctg tggttcagcc agaatgatat cgccttccat agcgaaagaa gctgtcacac
    23221 caccagtcgt tggatctgtc aaaatggtca ggtaaaagag accagcattt gaatggcgtt
    23281 taaccgctgc agagatctta gccatctgca tgagactcat gattccttcc tgcatacggg
    23341 ctccaccaga ggctgtgaat aggacaactg gtaatttttc gacagtcgca tactcaaaca
    23401 aacgagtgat tttttcacct acaaccgtac ccatagaagc catgataaaa ttagaatcca
    23461 taatcccaag agccacagtc tgacctttaa taagagcagt tcctgtcaca acggcttcat
    23521 gcagacctgt tttttcacgc atagatgcca gtttcttttg gtaaccaggg aaatgcaagg
    23581 gatccttgct ttcaatccct gtaaacaatt ctttgaaggt tcccatatca atcgtcaaag
    23641 ccaagcgttc ttgggcagaa atacgaaagg tatagctaca gtgcggacag atacgttcac
    23701 ttcccagatc cttctgatag atggtatgct tacagcctgg acactgggaa aataattcat
    23761 ctggaacctc tggcttagct tgaggttttt ccctaaccga acgattggga ttgattcgaa
    23821 tatacttatc ttttttacta aatagagcca ttgattcccc ttttcggttt aaactcttaa
    23881 agtcatttta ttctttttct tgatatttag gtaagaaggt ttccatcaag aaggaagtat
    23941 cataatcccc agcaatgaca ttgcgatctg aaatgagatc aagctggaaa tctgcattgg
    24001 tctgcactcc ttcaatctct aattcataga gggcacgttg cattttcatc aaggcgtcaa
    24061 aacgattttc gccgtgtact atgattttgg caatcatact atcataataa ggcggaatgg
    24121 tataacctgg ataaactgct gaatccacgc gcaagccaac tccaccactt ggcagataga
    24181 gattagtaat cttacctgga cttggagcaa agttaaaggc tgggttttct gcattgatac
    24241 gacactcgat ggcatgaccg cgtaggacaa tatcttcttg cttaacagac aaaggctgac
    24301 ctgccgcaat gcaaatctgt tccttaacga tatcaacacc tgaaacaaac tctgttactg
    24361 gatgttctac ctgaacacga gtattcatct ccatgaaata gaaattgcta cttgcttcat
    24421 caagaagaaa ttcaatggtt cctgcattct catagccaac aaactctgcc gctcgaacag
    24481 cagcagcacc tatttcatga cgcagcgttt ttccgattgc aatcgaggga ctttcttcca
    24541 aaaccttttg gttattcctt tgaagagaac aatcccgttc acccaagtga atcacatgtc
    24601 catgctcatc acctaggatt tgaacctcaa tgtgccgagc tggatagata acccgttcta
    24661 tgtacatggc accattgcca taattggcct tggcctcact agaggcagtt tcaaaggcag
    24721 aaacgaggtc atctggtttt tcaaccttac gaatcccttt accacctcca cctgctgaag
    24781 ccttgagcat aacaggatag ccaatttttt cagcaacaat caaagcttct tcagagttat
    24841 gcacttctcc atctgaacct ggtataacag gcacacctgc tttaatcatc tgagcacgcg
    24901 cattgatctt atcccccatc atatccataa catgaccaga tggaccgata aacttgatac
    24961 ctacttcttc acacatggtc gcaaatttgg aattttcact gagaaatcca aaaccagggt
    25021 gaatagcttc tgcctcagtc aagactgcag ctgatagaac tgcattaata ttgagataag
    25081 actctgttgc cttgccagga ccaatacaaa ctgcttcatc tgccaacagc gtatgaagag
    25141 cttccttatc agcagttgaa taaaccgcta ccgtcgcaat ccccaattca cgtgccgcac
    25201 ggataatacg aaccgcaatt tcaccacgat tggcaattaa aatttttcga aacatggaga
    25261 acctccttag ttcccaattg caaaagtaag ggtaccactg gctgcaagct tgccatccac
    25321 ttcagccttt gcttcaacca cagctatggt gccacgacgt tttacaaaag tcgctgtcat
    25381 aaccaattgg tcgcctggta caacttgctt cttgaactta accttatcca taccagcgta
    25441 aaagaccagt tttcctttat tttcaggttt tgataactcc aacacaccag cagtttgcgc
    25501 caaggcttcc ataatcagaa cacctggcat aactgggtat tgaggaaagt ggccgttaaa
    25561 gaaaggctcg ttgatggtca catttttgat agcaacaatg gtatcctcgc tcacctccaa
    25621 gacacggtcc actagaagca taggataacg gtggggaagg gcttctttga ttccttgaat
    25681 atcgatcatt tgatacgtac caatccttta ccaaactcaa ccatttcttc gttagagacg
    25741 agaatttccg ttaccacacc atccttagga gctgggattt cattcatgac tttcatggct
    25801 tcgataatta ccaatgtttg accttttttg acactatcac caactgtaac gaaggcaggt
    25861 ttatctggtc cagcagccaa gtaaaccact ccaacaagtg gactctctac aagatttccc
    25921 tcactagcca cacttgcttc agctggagct ggaacttctt ctgctacagt ctctgctgga
    25981 gcagatgtag gagctactgg actcggtgtt gctagaacgg gtgctggagc gacttgagtt
    26041 gcaacttcag gcacaggtct cgcttcattc ttgctaaact gcaactcatc cgtcccattt
    26101 ttataagaaa attctctcaa acttgactgg tcaaattgag tcatcaagtc tttaatatcg
    26161 tttaaattca tacttatcta ttctcccaac gtttgaaagc aagaactgca ttgtggcctc
    26221 caaaaccaaa agtatttgaa atagcgtatg gaatttcttt ctccaagcct tgtccataaa
    26281 cgacattagc ttcgatataa tctgatactt cacttgtccc agctgtcatt ggtacaaagt
    26341 tatgacgcat agcttcgatg gtgacgatag cttctactgc acccgcagcc cccagcaaat
    26401 gtcctgtaaa agacttggtt gatgatacag gtacttcctt accaagaaca gctacgatag
    26461 caccactttc tcctttttca ttggcaggag ttgacgttcc gtgagcattg acataggcta
    26521 cttgctctgg agaaatctca gcttcttcca aggctagttt gatggccttg atagctccct
    26581 gaccttctgg atgtggagaa gtcatgtggt aggcatcaca agtatttccg taaccaacca
    26641 cttcagccag gatagtagct ccacgttttt cagcgtgttc aagactttct agaaccaaca
    26701 tccctgaacc ttcacccata acaaacccat tgcgatcctt atcaaatggg atcgaagcac
    26761 gagttggatc ctctgtagta gagagagctg ttaaggcttg gaaaccagcg atggcaaaag
    26821 gtgtgataga agcttctgtt cctcccacca acatcacatc ttggaaacca aacttaatgg
    26881 agcggaaggc atccccaatc gcatcatttg atgaagagca ggcagtattg atagatttac
    26941 aaacaccgtt tgcaccaaaa cgcatggcta cattcccaga agccatattt ggtaaagctt
    27001 ttggaagagt cattggtttg acacgtttgg gtcctttttc atgaaggcga agtacctgat
    27061 cttcaatttc cttgattcca ccaataccag atgcaacgat aacaccaaaa cgatccctat
    27121 taagagcctc tacatcaaga ttggcatgat ttacagcctc ttgggctgca tacaaggcat
    27181 ataaagaata gttatcaaaa cggttggtat ctttttttac aaagtattta tcgaacggaa
    27241 aatcttggat ttctgccgca ttatgcacat caaagtcact atgatcaaat tttgtaatgc
    27301 caccaatgcc gattttccca gttgctaaac tattccaaaa ttcttctggt gtatttccga
    27361 ttggagatgt tactccataa cctgttacca ctactcgatt tagtttcatt cttttcacct
    27421 ctagctttcg ctacatactt aagccaccat caatggcaat cacttgacca gttagataat
    27481 cttggcctgc taaaaatact gtcaaatctg caacctgctc tgcctgccca aattctttca
    27541 tcggaatctg agctagtgta gcttccttaa tcttatctga taagatagct gtcatatcag
    27601 actcaatcat tcctggagca atcacattga ctcgtatatt ccgactagcg acctcgcgtg
    27661 ccacagactt ggtaaagcca atcaagccag ccttagaagc agcatagtta gcttgaccaa
    27721 tattccccat caaaccaaca acactagaca tattaatgat agcaccttct ctggctttca
    27781 tcatcggttt caagactgat tgtgtcatat taaaggcacc agtcagattg accttgagca
    27841 ctttttcaaa atctgcttct gtcatcttga gcataagagt atcttgggta atccctgcat
    27901 tgttgaccaa aacatctact gaacccagtt ctgcaatagc ttgatcaatc atacgcttag
    27961 cgtctgcaaa atctgataca tctcctgaaa tgggaaccac cttgatacca tagtttgaaa
    28021 actcagcgag caattcttct gagattgccc cacgactgtt taagacaatg ttggctcctg
    28081 cttgagcaaa cttgtgggcg atggcaagac caattccacg actcgaacct gtaataaaga
    28141 tatttttatg ttctagtttc atttttttcc tttcaaaact tctacttatt ttagtctatt
    28201 tttctaaaag tgctactaaa ctcgcttgat cttccacatg agctaagtga gcagtttgat
    28261 caattttttt aacaaaacct gacaagactt tccccggtcc aatctcgata aagttgctta
    28321 tgcctgcttc ttgcatgacc ccaatacttt catagaaacg aacgggttcc ttgacctgac
    28381 gcgtcaagag ctgagcaatg tcctcttttt gcatcacagc agcttctgta ttgccgacta
    28441 ggggacaagt aaaatctgaa aaacttacct gagctagagt ttcagctagt ttctggctag
    28501 caggctcaag gagagcggtg tgaaagggac ctgacacctt aagaggaatc aagcgtttgg
    28561 cacctgcttc ttgcaaaagt tcaaccgctc gatcaactgc aaccacttct ccagcaatga
    28621 cgatttgtgc aggtgtgtta tagttggctg gagtaaccac tccaagttca gaagcttttt
    28681 gacaggcttc ttcaatgacc tctactggcg tattgagaac tgctaccatc ttgccagagt
    28741 cagcaggagc cgcttcttcc atataggctc cacgcttagc taccaaggca accgcatctt
    28801 caaaatccaa ggcgccgctt gccaccaagg cagagtattc tccaagagac aaaccagcaa
    28861 ccatatcagg ctgatagccc ttttcttgca ataaacggta gatagcaacc gaagtcgcta
    28921 gaatggctgg ttgcgtatag cgggtctgat tgagtttgtc ttcttccgta tcgatgagat
    28981 aacgtaaatc ataaccgagc acctgactcg ctcgatcaat cgtttcttta acaatcggat
    29041 actgatcata gaaatcccgt cccatcccta gatactgggc accttgacca gcaaataaaa
    29101 aggctgtttt agtcatttct tacaactcct gcccagcgag aggcttcttc ttgaattttc
    29161 ttagcggctc cgtaatacaa atcttttagg atttcttcag ctgtttcttc tttagaaaca
    29221 agccctgcga tttgacctgc catgacagag ccaccctcca catcaccgtg aacaactgct
    29281 ttggctaggg cacctgctcc catttgttca aagatttcta aatcaggatc ttcctgctta
    29341 aaggcatctt tttcagccag ttcaaaatct ctagtcaact gatttttaat agcacgaaca
    29401 gcatgaccaa agtgctgagc tgaaatcgta gtatcaatat cccttgcttt taaaattttc
    29461 tctttgtagt ttggatgggc attcgactct tttgcaacta caaaccgtgt ccccacctgt
    29521 acagcctctg cacctagcat aaagccagcc gcagcacctt caccatccgc aattcctcct
    29581 gcagcaataa caggaataga tatagctgtg gctacctgtc gcaccaaggt catggttgtt
    29641 aatttaccga tatgcccccc agcttccatt ccttctgcaa taacagcgtc tgcaccgatt
    29701 ttttccatgc gtttagctaa agcgacacta ggaacaacag gaataacgat tatcccagct
    29761 tcatggaaac gttccatata cttgcttgga tttcctgctc ctgttgtgac aactttaaca
    29821 ccttcttcaa taacgagatc cacgatatct tccacaaagg gagataagag catgatgttg
    29881 accccaaagg gtttatcagt caatgatttg attttatcaa tattggcctt gacaacttct
    29941 ttcggggcat ttcccccacc gataattcct aatcctccag ccttggaaac agcccctgcc
    30001 aaatcaccat cagcaaccca ggccatccct ccttggaaaa taggatagtc aatcttcaat
    30061 aattctgtaa tacgcgtttt catagtgcct ccaaccttcc ttgcttacgt aatagttcga
    30121 tttcaccata atttgacagt caaactatta cctaaacaag agggagtggg tttctcccta
    30181 ctccttctac taatattctg cttattttgc ttgctcttca acgtaagcaa ccaagtcacc
    30241 aactgttttc aagtcatttt ctgcttcgat ttggatatca aaagcatctt cgatttctga
    30301 gattacttgg aacaagtcca atgaatctgc gtccaaatca tcaaaagttg attcaagtgt
    30361 tacttctgat gcgtcttttc caagttcttc aacgataatt tcttgtactt tttcaaatac
    30421 tgccatgata ggactccttt aaaataaata gtttttttat aacaatgtgt tcaccacatg
    30481 attacctaaa ttgtaagaat gagcgtgccc caggtcaagc ctccaccgaa gcctgataga
    30541 agaacagttt ggctaccatc taaagggatg agaccttgtt ctacacactc tgaaagtaaa
    30601 atcgggatac tggctgcact ggtattgcca tattccatca tattagctgg aagtttggct
    30661 cggtcaacac caatttttct agccatctta tccaaaatac ggttattggc ttgatgaagt
    30721 agcagataat ccaagtctgt cacctctata ggagattcat caatagtctg cttgatagac
    30781 ttggctacat ctcgaatggc aaaatcaaag actgtgcgtc catccatctt caaaaatgaa
    30841 tctgcacttt cttgatctga aaatggagaa tgcaaacctg aatgcccata agttaaacac
    30901 tcgctgcgac ttccatcgct attgagactc tcagctaaga aatgctcttg ctcgctagct
    30961 tctaacaaga caccaccagc accatctcca aacaacacag ctgttgatcg atccgaccaa
    31021 tcgactgcct tagagagggt ttcactacca atcaccaagc ctttttgaaa gcgaccagaa
    31081 gcgataaact tttcagcagt tgaaagagca aatacaaatc cactgcaagc cgcggttaag
    31141 tcaaaagcaa aggctttatt agcaccaata ttagcttgaa cacgagcagc tgtagagggc
    31201 atcatcgaat ctggagtaat ggtagctagg atgataaaat ccagttcttc tcctgttatt
    31261 ccagcttttg ccatcagttt cttagcaacc tctgtagcca aatcactggt agattctgtt
    31321 cttgaaatat gcctttgtcg tattcccgtt cgacttgaaa tccactcatc attggtatcc
    31381 ataatctgag ccaagtcgtg atttgtaacc acttgctctg gcacataatg agcaacctga
    31441 cttatttttg caaaagccat tatttcaaat cctccaaaaa ttggtaaaga ttagtcaaac
    31501 ctttacccat gacagcaatt tcttcctcgc tcatgccatc aataattttt tctaccatgg
    31561 ccttgtggaa gcgtttatgc agtctatgaa tcaagcgacc cttctttgtc aaatgcagat
    31621 gcaccacacg acgatcctgt tctgaccgaa ctcgctcaat gtagccctta cgctctaaat
    31681 tgttcaaact tgtcgtaaca gttccaagag ttaccatcaa ctcttttgac acttgacttg
    31741 gagtcacatc tggagcctta ccgatgacat caatcgtatg catttctttg atggagatat
    31801 ccttaaaacg actacctctc aagttcactt cctcaattac aaggacattg ttaaatatag
    31861 atgttaaata ttcattgatt cgttggtagt ccatttttca tatccctcct tcttcaaaaa
    31921 acttttacaa tcaaaacatt tgacagaaag aattatatca aaattcaaag aatgatgcaa
    31981 gtatttttta ttttcctgta aatttaggtc ttcttctctc cgaatgagct cgcactcctt
    32041 ctttgaaatc ctctgtttga gctaaggatt tctgtaggtt cagttctaaa gtagcgtaac
    32101 cctgccagtc tttaaattgg ctctcccaaa ccaacttctt aatggcagca taggaattac
    32161 ttgaagcacg tcttaatttt ttaagaagct gttctctcgt cttttcaagt ttttcagctt
    32221 cagaaacgcg gtaaaccaac ccccattcta gggctttttc tgccgttaaa gcctctcctg
    32281 tcatagctaa ttgagcagca cgcgtcacac cgatactgcg gctcaagaga tgaattcccc
    32341 ctgcatctgg agccaaacca acaccaacaa aggcttggat aaacttagcc ttatccgtcg
    32401 ccaaacagaa atctgccgca acagccatat tcgctgcggc acctgcaaca gcaccgtcaa
    32461 cttccattaa aacaggtttg gctatttgct tgattttata agaaatcgta ttgaccaact
    32521 ctgcgatttt tgtcaatgat ggaatatcat cctcatccac tgcccgcttc atctctacca
    32581 aatctccccc aactgagaag acctttccat tggcattgat taagataaaa tgcacagctg
    32641 gattttcttc tgccaaagtc agagcttcta aaatctcctc acacatggga atatgaaaac
    32701 catttgcgac ctcaggacgg ttcaaggtaa ggattgccaa gtcctcttca agctgataaa
    32761 taatgtgttc catcagggct cctttttatt ttataagcag tttatactct tcgaaaatct
    32821 ctttaaaccg cgtcagcttc catctgcaac ctcaaaacag tattttgagc tgacttcgtc
    32881 tagcttctta gtttgctctt tgattttcat tgagtgttaa attatttcac tgtcaaagta
    32941 tatctttttt aggagcagaa agcaagaaaa aatcgactga aagtgtctca gccgataaga
    33001 aaggctcgct ttaggctaga aagcatctta ttttcttcat tgaaaaaagc ctttctttct
    33061 gctataatcg tataaaatac ttactttagg agttcttatg aaagttgtta aatttggagg
    33121 tagttctctt gcctctgcta gtcaattaga aaaagtttta aacatcgtca aaagcgattc
    33181 agagcgtcgt tttgtagtcg tttctgcgcc tggtaaacgc aatgctgaag atactaaggt
    33241 tacggatgcc ctgattaaat actaccgcga ctatgttgcg ggtaacgata ttagcaagag
    33301 ccaaagctgg attatcgacc gctatgctgc tatggttagt gaattgggac taaaaccagc
    33361 tgtgctagaa aaaatttcta aaagcattca cgccttggcc actcttccta ttgaagaaaa
    33421 tgaatttctc tacgatactt tcctagcagc cggtgaaaat aacaatgcca aattgattgc
    33481 tgcctacttt aaccaaaatg gtatcgatgc acgctatatg caccctagag aagctgggat
    33541 tgtggtcaca agtgaacctg gtcacgctcg catcattcca tcaagttatg acaagattga
    33601 agaattgaca aacaccaatg aagtccttgt cattcctggt ttctttggtg tcactaagga
    33661 aaatcaaatc tgtactttct cacgtggagg ttctgatatt acaggttcta tcattgctgc
    33721 tggtgtcaaa gctgacctct atgaaaactt tacggacgtt gatggtatct ttgcagccca
    33781 ccctggtatt atccaccaac cacactcgat tcctgagttg acctaccgtg aaatgcgcga
    33841 gttggcctat gcaggcttct cagtccttca tgacgaggct cttcttcctg cctaccgtgg
    33901 aaaaattcct ctggttatca agaataccaa caaccctgac catccaggta ctcgtatcgt
    33961 tctaaaacac agtaatgatg aatttccagt tgtgggaatt gctggtgact caggctttgt
    34021 cagcattaac atgtcgaaat acctcatgaa ccgtgaggtt ggatttggcc gcaaggttct
    34081 gcaaatcctg gaagaactta acatcggttg ggaacatatg ccaacaggta tcgacgatct
    34141 ttctatcatt ctccgttctc gccaactaac tcccatcaag gaagaagaaa tcctgcgtca
    34201 gttggttcaa aaggctaaag tagatcatgc agaaatcgaa cacgaccttt ctatcattat
    34261 gattgttggt gaaaaaatga agagccatat cggagtgact gctactgcga cacgcgctct
    34321 atctgaaaac aaaatcaaca tccagatgat gtctcaaggt tctagtgaag tttctattat
    34381 gtttgttgtc aataaagacc aagagaaagc agcaattaaa gccctctaca atgccttttt
    34441 tggagaaagt aaggaagact aagcatggcc caatcactta acaaaacagt actcctcagt
    34501 acgacaggca cttcctacct ctctatcgct gggaaagttg ggaaattcct tgtcggagat
    34561 caggctttgg aattttaccc agatgtcaat gtcgaacaat ttatccagat tccttggagc
    34621 catatcaatc agattggagc caatgtcact ggacgcaaaa tcagccgcca cttcgaagtc
    34681 tttacagaca aaggaaaatt cctctttgct tcaaaagact caggtgccat tctcaaaatt
    34741 gcacgcgaaa aactaggcaa tgacaaggtc gtcaaacttc cgaccctgat tcagacaatt
    34801 agccaaaaat ttaaaaatct atttgcaaaa aagtagaaac tttagtataa tcatagcaac
    34861 ggataagtag gttatcttct tctttcagaa agtctgcggg tactgcgagc agataggggg
    34921 gataggtgaa attctaccgt tagtaacaat tacatacaca atcaagtgca cacctgtgaa
    34981 gttggatgga accgtggccc tgccactcca acgttttgtc aggcgtgctt ttttcataaa
    35041 ggagttctta tgttagatat caaacgtatt cgtacagatt ttgaagctgt cgcagaaaaa
    35101 ttagctacac gtggtgtaga tgctgctgtc ttgaatgaaa tgaaagaaat cgatgctaaa
    35161 cgtcgtaaca tcttggtcaa ggttgaaact ctcaaagcag aacgtaacac agtttctgct
    35221 gagattgccc aagctaagcg caacaaggaa aatacagatg acaagattgc tgccatgcaa
    35281 aatctatctg ctgaggttaa agccttggat gctgaattgg cagaaatcga tgctaaattg
    35341 acagaattta caacgactct tccaaatatc ccagctgaca gcgttcctgt tgggactgac
    35401 gaagacgaca atgtggaagt tcgccgttgg ggtactccac gcgagtttga cttcgaacct
    35461 aaagctcact gggatctcgg tgaagacctt ggtatccttg actgggaacg cggtggtaag
    35521 gtaacaggcg ctcgcttcct cttctataaa ggcctcggtg ctcgtttgga acgtgctatc
    35581 tacaacttta tgttggatga acatggaaaa gaaggctata ctgaagtcat cacaccttac
    35641 atagtcaacc atgattctat gtttggtact ggtcagtatc caaaatttaa ggaagatact
    35701 tttgaactca gcgataccaa ctttgtcttg attccaactg ctgaagttcc tctgacaaac
    35761 tactaccgtg atgaaatctt agacggcaaa gatcttccaa tctacttcac tgccatgagt
    35821 ccgtcattcc gttctgaggc tggttctgcc ggtcgtgata cgcgtggctt gatccgtttg
    35881 caccaattcc acaaggttga aatggtcaaa tttgccaaac cagaagaatc ttacgaagaa
    35941 ttggaaaaaa tgacagccaa cgctgaaaac attcttcaaa aactcaacct tccataccgt
    36001 gtcgttgctc tctctactgg agatatgggc ttctcagctg cgaagactta cgacttggaa
    36061 gtgtggattc cagcacaaaa caattaccgt gaaatctcaa gctgttcaaa cacagaagat
    36121 ttccaagccc gtcgtgccca aatccgttac cgtgatgaag cagatggcaa ggtgaaactc
    36181 cttcatacct tgaacggctc tggacttgca gttggacgta cagtggctgc aattcttgaa
    36241 aattaccaaa atgaagatgg ttctgtgacc atcccagaag cacttcgtcc atacatgggt
    36301 ggagctgaag tcatcaaacc ataaaaaata aggtttagct atttctagct agaccttttt
    36361 tcgtaaccaa atcagataag cacctagtac aaagaataaa atagttaggc atataatggt
    36421 ttcagccaat accaggtaat ccagaaatgg aagtttcaaa attccctgag ccatcttgag
    36481 cgaggtcgct gtgataatgg ttgggaaggt gagggctgag agggctggtt gaaaaccttg
    36541 ttttaaaatg ttgggcagac gagttaaaac aaagaaaaag aaggattgag aagccaaaat
    36601 catgacaatc aagacccaag tcggcaggct ggttcctcct actcgaacta gagaagccaa
    36661 gagtagagag aaaggagcac agtagattcc ttcttgtcca agcaaggcta gtgggagtgg
    36721 atgtttcttt aaatcgctat aaataagggg atagagatag aaggtcaaga gaaaaccaaa
    36781 actcaaggtc gcataggcaa tttcgataat acctaccaga ggataggtca aggcagccac
    36841 tgctatcccc acatagagaa ccgtccaact tggagtggca tgaaccctcc gccctgaaca
    36901 agcaaacttg atggtaaaac cagcaatcaa ggtcaaatcc aagagaaatg aaaaccacca
    36961 aatcccttgt gctaccaaag gaagataaga gaatacgcga aagacatagg tcgataaaat
    37021 catcccagcc ataggaaagg ttgccattcc tgacaaaaga gggggcttgg tcaattcttg
    37081 cttggtttct ttccaattaa agagatgcag aattagaaag taaatccata aaaccaaacc
    37141 agtcagacta aaaagatggg atagaaccgg caacgtatct aaaataagat ttccagctcc
    37201 tgccaaacct agcaaacaac ctgaaaatac taaggggagt tttttcatcc taacctccaa
    37261 taatcatgtt agtttcagta taacataaaa gcgcttaaat gaggatttaa aaaaacgaag
    37321 tccgcttatt tcagacttca ttttactcag atatgaatta ggcataaggt tgcaattctg
    37381 gattaattgg tgtattagct aagttgttgg catagttaca gaggattgct aggctgacac
    37441 caaaaaccac atccaaggca ttttgttgag tgtagccagc ttctaaaaac tcagacaagg
    37501 cttcatctcc tacacgaccc ttggtattga taactgccaa ggtaaactta gctagggtat
    37561 ccaatttagg atctgtttca attggagtac gattgcgaag agcttgaatc aagtcatcat
    37621 tcatctggat ttgtttgatg gaaaaggctg tgtgacctgc gacacagaag gcacaaccat
    37681 tggtcacggc tgccgtgatt tgcaccactt cacgctcaac gggtgtcagg ctgttgcgac
    37741 ggtggataga tgagacaatt tggtaggctt ctaaaacagt cggggcattg gccaagagac
    37801 cgattaggtt gggaatatag ccattgttgt ctttttctac tgtttcaaga atttctttca
    37861 cttctgctgg tgctgactct actgtatgga tagtaaatgt tgtcataaga tacctctttt
    37921 cttattattg acactaatat tattggaaaa tcttataaaa tcctgattcc taagtttatc
    37981 taagataaag ctttattctc tcataagatt ttcgttgtta tattagttta tcacacttcc
    38041 aatcacttgc ataatatata ttatatatca ggctgataaa aattatttat aggcaaaaaa
    38101 atcacacgag ctgtgtgatt ccattatttg tcaaaatact ttttagtttc agcaataacg
    38161 actggcgaca agaccaagag ggcaatcaag tttggcagag ccatcaaggc gttaacgata
    38221 tctgcgataa tccagaccat atccaactcg ataaatcctc ctaacaagac catgagcaca
    38281 aaaaacacac ggtagagcca gataaagcga gccccaaaga ggaactcaaa acagcgttct
    38341 ccgtaatagt tccaacctag aatcgttgta aaggcaaaaa gtacaaggaa gatggtcaag
    38401 agggcaggcc caaagtgtga aaagactgtt gagaaagctg actgagtcaa ggcaacccca
    38461 ttcaagtcac cactccaaac tccagttacc aagatggtca aaccagttag agtacaaatg
    38521 atgagggtat caataaaggt tcctgtcatg gaaatcaaac cttgctctac tggttcattt
    38581 gtcttggcag ctgcagctgc aataggagca gaacccagac cagattcgtt tgagaacaca
    38641 ccacgcgcca caccattttg aatagccatc cgaacgctag caccagcaaa tccacctacc
    38701 gcagcaaggg gactaaaagc tgaggtaaag actaaagcga ttgtgccagg gatttttccg
    38761 atattaaaga aaataactgt aagagttcct aagatataaa tgagggccat aaaaggaaca
    38821 acagtagttg aaaccttaga aatagacttg agtccaccaa agactgcaat cgctacaaag
    38881 acagacaaga cgagagctgt gatggctggc gaaatcgtcg ttgtattttg gatagattct
    38941 gtaatcgagt tgacttgggt gaaggttccg attcccaaga gagcaaccaa tactcctgct
    39001 actgcaaaca aaacagcaag tggtcgccac ttttctccca tccctagaag gatatagtgc
    39061 atgggacctc ccgctactgc accatggtcg tccttggtgc ggtatttgat ggccaagagt
    39121 ccttccgcat acttggtagc cattccaaag aaagccgcca tccacatcca aaatagagct
    39181 cctggtccac caaccttgat agccgtcgca actcctatga tatttcctgt tccaacagtt
    39241 gatgccaagg ctgtacacag agctgcaaaa ctggatacat caccatgtcc cttatcctgg
    39301 ataaaaataa gctgaaaggc cttgggcaga cgcaaaacct gcaagagtcc tagccgcatg
    39361 gttaggtaaa tccctgttcc gaccaataaa atcaagaggg gcggtcccca agcaaaagca
    39421 tcgattgatt taagcaattc taacatttcc ttctcctatc gtttcaaccc caaaagaaag
    39481 agcacatgca agatacatgt actctggaat gcttagataa atgctaaaaa gcggtctatc
    39541 ctagctctgt ccttttacct gagagtttga gcagttgcct gccttgcccc ttcggtgcct
    39601 ttacggtctc tccagagttc cgtccattta cagtcatgga aaatcaaacg attccccact
    39661 tctattaaac ttcattcggt gttggtattt aattgattct aattttacaa aaaatgttgg
    39721 cttttgtcaa tgtgtttatt agtaaaaatt agttcaacag tttttacttt ataaagtcca
    39781 gaatactgct atcctttaaa agtgacaata gtcgcaccac tgcctccagc attttgtggg
    39841 gcatagccga aactcttgac atgtttgttt ctttgcaagt atttggtaac tccttcacgg
    39901 atgactcctg ttccgatacc atggatgata tcaacttgag ccatattgtt aagcaaggct
    39961 tggtcgatga aggtatctag ctcattcatg gcttcttcat agcgcttgcc tcgaagatcc
    40021 agtctagctt gaggtcctcg cccagaagtt cgtttcacaa cattgacctg tttcttcttg
    40081 actggttttt cttgctgggc ttgaacaaga tcaaactctt tctcttccaa ggtcatctta
    40141 atcaagccaa cttgggcttc ccagcgaccg tccttgagtt gactggtcaa ggtaccacgc
    40201 tgaccataac tgagaaccac gatatcatct cccacctttg gagctcgttt tttcttggcc
    40261 ttttgaagga ccttattttt agacaagtcc actttttcag gagccaattt tttcaacttg
    40321 gccttggctt caatgatttc gtggggcttg agttgggatt tactgtggag atttttgaga
    40381 atctggtcac tttcacttag ggccatatcc acaatctcag cagcctgttc acgcgccttg
    40441 ttaagctcgg tttccttttc acgattaagc tcgttgtaga gtttttttag cgcacggttc
    40501 atcttgagat tttcttgctc cacctcacgg atattgtcca aacgtttgcg gctttccagc
    40561 gtctgctctt ctaattgctc aatgatacga ttgacgtcat tgtcctgatc gatctgctga
    40621 ctggcatctc ctacgataac ttcagatagg cctagacgtt tggcaatttc aaaggcatta
    40681 cttcggccag gaacaccctg cataaagcga taggtcgggc gaagagttgc agtatcaaac
    40741 tccatactgg cattttgcac aaaggctgtc tcaataccgt aggccttgag ttctggatag
    40801 tgggtcgtcg ccatggtctt gatttgacgc aggcgaaggt cctccagaat agccatggca
    40861 agggctgctc cctcttgggg atcagtacca gcccccaact catccaaaag taagagtgaa
    40921 tgttggttga ccttgccaag aatatccacg atattggtca tatggctaga gaaggtagac
    40981 aagctctgct caatagactg ctcatctcca atatcagcaa agatttcttc aaaaatacca
    41041 acacgacttc ccttgtctgc taaaatcggc aatcctgact gggccatgac ctgtgtcaag
    41101 cccagagttt tgagcatgat ggtcttccca cctgtattgg gacctgtaat gacaatagct
    41161 gttaaatctt gaccaaaatg gacatcattt gcgacggcat ttttgaccaa aggatggcag
    41221 acatggagca gttgaatctc ttgattttct gacagctgag gcacgactgc ttgtctttct
    41281 tggataaatc gaaccttggc acgaatcaag tccagatgac cgataatcca agcgtcatta
    41341 gcaatctcag ccgcatgagg gcggacacgc tcagaaattt cttggagaat gcgaagcatt
    41401 tcatagcgct catctgctcg cagactagca atttcttcgc tcagtttgac tacctcacgt
    41461 ggttcgatat agacggtgtt tccactagca gaaatatcat gaacgacacc tgcaatctta
    41521 ttgcggtagg tgtttttgac tggtaaaacc tgacggccat ttctgctagc aacaattcct
    41581 tccgtcaaca tctgcgcttt ttgcttgagc aagtcttgta aaacatcgcg tacctgactc
    41641 tcgctatcat gtatttttcg acggattcgc gccaattctt cactggcaaa attttcaatg
    41701 aaacccgcat cattaaaggc ctgaagattt ccttgtaatt gcggaaaatc atgtaatttc
    41761 tcaaaccaaa gggctaattc ttccaagctg acattttcca gattggcata aaaactttga
    41821 agttctcggc tggcaagaag cacgcgtttc aagagtagga actcctcgat attgagatcc
    41881 gctcccatct ccaacctctt gcagactcct gcaatttcct tagttgagag aatagtaaaa
    41941 tgcggttgct cgacgaaaag agcctgcatt tctttcatct cagcaaaagc ctgtttgatt
    42001 ttatctgctt tggcagtcgg agccagctgt ctcaattgct ccaagccctg ctcggtcaac
    42061 aaatgaggat caaacaaggc cttgacctta tcgaactcta atgtttctaa tattttctta
    42121 ttcatcttta tttccttgtc tttgaatgtc taggtgtggt agctttttac attctgatag
    42181 attcgagtca aatctgtaaa caaagggcta ggaaaactcc tgcccttttt atccgattaa
    42241 atttgtcacc cagatttgtt tgagccaact ggttgttatc ggtatgctct ggatgatgtg
    42301 ttttgcgacg atactctttt caagaggatt ttgtataact gccatgggga tggtcgccaa
    42361 gattgtcaag gccatttgca agacaaataa ggtcaccaac atggacaaga tacctgctga
    42421 aacttggaac aacttaccac ccagtttttt gctaggaatc aagtgtaaga gaagaccaag
    42481 caaacgacca atgctataga aaatcccaaa tacaagcaag tagccgatac ctgcataaaa
    42541 gaccttatcc agctgaaaga gttgatccga tgggaaaaag aaagtcccct gaccttcctg
    42601 cgaatttgca taagggagca ataaatggaa ttgctctcca agccccttat aaaactggcc
    42661 agccataaaa gccgatgcca tggctgaaat caggtaataa acctgtaaga gcaggcctct
    42721 ccgatagccg atataaaatc cccaaaccaa gaccaataga agaaggaatg aaatcataag
    42781 gaatcctcaa tcttgctctg ttcttgctta caagtcacaa gcttgtgacg gagttcttct
    42841 agctcttgct ccttatcgtc aaattcaatc tcacggctga gctgagttga taaacagttg
    42901 actgccaaca aaagagcgat tgtttcatca tctgcgctag gcatttgttc tttaattgct
    42961 tggtattttt ctgtcgcaac cttagcgatt tcctccataa aaaggttatc atgttcgctt
    43021 gtcaaggtta acgatttttt cccgaatgta aatttgaatc gatttagatt tgccataaaa
    43081 ttcacctcac gatattatac caaaattcgc taattttgtc agtttttaca aattttactg
    43141 cttttgtggt acaatagaaa ctatggcaag tatcacactc acaccaagcg aaaaggatat
    43201 tcaggctttt cttgaacact atcaaaccag tctggctccc agtaagaatc cctatatccg
    43261 ctactttttg aaactacctc aagcaacggt ttctatctat acttctggaa aaatcttgct
    43321 tcagggtgaa ggggctgaga aatacgctag tttctttggc tatcaacttg tagaggaaaa
    43381 cagggggcaa aatcttcctt taattgggac agatgaggtg ggaaatggtt cctactttgg
    43441 tgggcttgca gttgtggctg cctttgtcac atctgaccag cacgactttt tacgaaaact
    43501 cggtgtgggg gattctaaga ctctgaccga ccaaaagatc cgtcagattg ctcctattct
    43561 caaggaaaaa atccagcacc aggcactcct tctctcaccc agcaagtaca acgaggtcat
    43621 cggagaccgc tacaatgctg tttcggttaa ggttgccctc cataatcagg ctatctatct
    43681 cctccttcaa aaaggtgttc agcctgagaa aattgtgatt gatgccttta ccagtgctaa
    43741 aaattatgac aagtacttgg cacaagaggc caatcgtttc agcaatccta tcagcttaga
    43801 agaaaaggct gagggcaaat acttgtctgt cgcagtttct tctgtcattg cgcgtgatct
    43861 ctttctggaa aatcttgaaa atttgggacg agaactgggt tatcagctcc caagtggagc
    43921 tggaacggct tctgacaagg tggctagcca gattttgcaa gcctatggta tgcagggact
    43981 cagcttctgc gccaaattgc actttaaaaa cactgaaaaa gcgaaaaaac gcttagaaag
    44041 gtaagttatg aattcattta aaaatttctt aaaagagtgg ggattgttcc tcctaattct
    44101 gtcattacta gctttaagtc gtatcttttt ttggagcaat gttcgcgtag aaggacattc
    44161 catggatccg accctagcgg atggcgaaat tctcttcgtt gtaaaacacc ttcctattga
    44221 ccgttttgat atcgtggtgg cccatgagga agatggcaat aaggacatcg tcaagcgcgt
    44281 gattggaatg cctggcgaca ccattcgtta cgaaaatgat aaactctaca tcaatgacaa
    44341 agaaacggac gagccttatc tagcagacta tatcaaacgc ttcaaggatg acaaactcca
    44401 aagcacctac tcaggcaagg gctttgaagg aaataaagga actttcttta gaagtatcgc
    44461 tcaaaaagcc caagccttca cagttgatgt caactacaac accaacttta gctttactgt
    44521 tccagaagga gaataccttc tcctcggaga tgaccgcttg gtttcgagcg acagccgcca
    44581 cgtaggtacc ttcaaagcaa aagatatcac aggggaagct aaattccgct tctggccaat
    44641 cacccgtatc ggaacatttt aagagaccta agaggccgag aatcaccaat ctcagcctct
    44701 tcttctatcg tgagaagtga ttgtttattg acaaatactc aacgaaaatc aaagaacaaa
    44761 ctaggaagct agccgtaggc tgcttaaaac agtgttttga ggttgtggat agaactgacg
    44821 aagtcagctc aaaatactgt tttgaggttg cagatggaag ctgacgtggt ttgaagatat
    44881 tttcgaagag tataaaataa aaacaatccc aactctcacc tattcatgca aaggaatttt
    44941 atggaagttt atttttcagg aaccattgaa cggattattt ttgaaaatcc cagcaatttt
    45001 tatcgcatcc tcctcctaga aatcgacgat acggacgcag aggattttga tgattttgaa
    45061 atcattgtca caggaaccat ggctgatgta attgagggcg aagactatac tttttggggg
    45121 caaattgtcc agcactccaa gtatggagaa caactgcaaa tcagtcgtta tgatcgcgca
    45181 aaaccaacta gtaagggctt ggtcaagtac ttttcaagta gccatttcaa ggggattggt
    45241 ctcaagacag ctcagaaaat cgtggatacc tatggcgaaa ataccattga cgaaattttg
    45301 caacacccag aaaagttaga aggcatcgca ggactctctg ccaaaaatcg cgaggctttc
    45361 gtctccactc tccgtctcaa ctacggaacg gagatggttt tgtccaaact agccaactac
    45421 ggcattccca acaaactagc ctttcagatt caagactttt acaaggaaga aacccttgat
    45481 gtggttgaaa attatcccta ccagctggtt gaggatatca agggtttggg ctttaccatt
    45541 gctgaccaac tagctgagga actaggcatc gaaagtcagg ctcctgaacg cttccgcgcc
    45601 ggtctagttc acagtctttt tcaggcctgt atggaaacag gggacaccta tgttgaagca
    45661 cgggatttgc tggaacaaac ccttactctc cttgagtctt cccgtcccgt ggaactagac
    45721 cccagccaag ttgcccaaga gctctcctac ctgatcgaag aagacaaggt tcagcagatt
    45781 gataccaaga tctttgacaa cagcctcttt ttcgctgagg aaggcatccg cagtcacttg
    45841 gttcgtatcc ttgaaaaagg aaaacagaag agtcatgatt tagaaaccat tcaaaaacat
    45901 atcactacta tcgaggaaga gctgggaatt gagtatgata acattcaaaa acaggctatc
    45961 tgtgatgcta tccagaataa ggtctttatc ctgacaggtg ggcctggtac tggtaagaca
    46021 actgttatca atggaatcat cgctgtttat gccctgttag agggacttga cttcaggaag
    46081 aaaagcaatc tgcctattct tcttgctgct ccaactggtc gagctgctcg tcgcatgaat
    46141 gaattgacag gtttgcctag cgcgaccata catcgccact tgggaatgac aggtgacgat
    46201 gataccagtc atctggaaga ttacctagat gctgacttta tcatcgtgga tgaattctct
    46261 atggtagata cttggctggc caatcaactc ttctccaata tctcttctaa cagtaagatc
    46321 ctcatcgtag gtgacagtga tcagctacct tctgtcagtc ctggacaggt tctagcggat
    46381 ctgcttcata ttcctttgat tcctcagact cacttggaaa aaatttatcg acaaagcaaa
    46441 gaatcaacca tcgtcaccct agctagtcag attcgacagg gcatcttgcc agctgatttc
    46501 acccaaaaaa aagctgaccg ttcctacttt gaaattgcta gtggccatat tcctgccacc
    46561 attgaaaaaa tcttaggtgc cgccctcaga aatggtattc ctgcccgtga tatccaagtt
    46621 ctggctccta tgtaccgagg gacggcaggg attgatgcta tcaatcagct catgcaagac
    46681 ctgctcaatc ccccacaaaa agatcaactc agttttgaag ctcctcagtg ccactatcgt
    46741 aagggagaca aggtcattca tttggttaac gatgctgaaa tcaatgtctt taatggggat
    46801 ttaggagcta tcacagacct gattcctggt aaatacaccg aatcgaaaca agacgagatt
    46861 gtcattgatt ttgatggtaa tgaagtctct tacccccgta acgaatggta caagattcgc
    46921 ttggcctatg ccatgagtat tcataagtct cagggaagtg agttcccagt tgtcatccta
    46981 ccgattacta gtgctagccg acgtatgctg gagcgaaatc tcatctacac agccattaca
    47041 cgtgccaaaa gcaaactcat cttactaggc gaattacaag ccttcgacta tgctacccaa
    47101 cacatcggaa ctgcccgaaa aacctatctg attgaacgct tcagtgatct actggagaat
    47161 gttgaagaaa agcaacaagc tgtctctgaa acagtcacat caagtgcctc tgaacaatcc
    47221 tacatcctaa ccgaagaaaa ctgggacagc attccagcca tgattgggat tacagacaca
    47281 gacctcaaag agatttttgg aaaatagtgc atcagaaaaa ccaccaattc aggtgggttt
    47341 tctgtttatt aagattattt tactgttgct gtgcttgtaa tcaattcaac agcttttttg
    47401 atagtgatat catgtttcaa catgtcagct gaaagcaagt tttgaacttg tgcaacttcc
    47461 atgttgtagt ctgctgccaa ttgctcaact tctttttgga tttcttcttc tgaagcatca
    47521 aatccttcag ctttggcaac tgcttcgata acaaggttag tcttagtacg tgactcagct
    47581 tctgcttggt attggttgtg aaggtcttct tgagtagttc cagtaatttg gaagtacatg
    47641 tcagggttga tcccttgacg ttgcaagttc ccaaggaatt catttactga acggtgaact
    47701 tcttcgtgga tcatttcttc tggaagttct acgatttcag cattttctac agctgtatca
    47761 attgctgcac cttcaactgc atctttgtaa gcttcttctt tagcagcagc caattctttg
    47821 cggtattttt ctttcaagtc agcaagtgtt tcaacttctt catcaatgtc ttttgcaagt
    47881 tcatcgtcaa gagctggaac ttctttagct tttacttcgt ggatagttgt cacgaattta
    47941 gcttctttac ctgcaaggtc ttctgcttgg tagtcttctg ggaatgttac gataacatca
    48001 acggtttcgc cagctgagtg acctaccaat tggtcttcga aaccagggat gaattgacct
    48061 gaaccaagtc caagtgagaa gttttcacct tttccaccgt caaattcaac accgtcgata
    48121 gaaccaacga agtcgatcac aacagtgtcg ccgtcttcag cagcagcttc cttgataacc
    48181 aattcagcca ggttgttgcg ttcacgttcg atacgctctt cgacatcagc gtcagttact
    48241 tctttttcta catcaactga tacttcaagg tttttgtagt cacccaattt tacttcaggt
    48301 tttgtaacaa cttcagcagc gataacccag tcttgacctt tttccattga agttacgtca
    48361 atttttggtt gggcaaccac ttcaagacca gcctctttta cagctgcttc ataagcgttt
    48421 ggcaaaagag cgttcataac gtcttggtaa agtgattctt caccaaattt tttatcgaag
    48481 ataggacgtg gaaggtgacc tttacggaaa cctggaacat taagagattt cttcactgac
    48541 ttgaagacac ggtccaattc tggtttgatt tggtcttgag agatagtgaa agtcaagaca
    48601 ccacggtttg tttctttgtt ttcaaatgat acagacattc tgtcatttct ccttaaaatt
    48661 ttttaaatac agtctattat aacattttct ataaataaaa tctagactaa tgcagacaat
    48721 tatagacaac aagccttttt cctaactaaa acactcataa agtatagaca attttagact
    48781 actttggata tcttttggtt agtcattttc caaaactaat ttatctaatt tttaagttat
    48841 atttgacttc atcttctcag taacatgagt ataaacactt aaaatctttg tttcattagt
    48901 ataatctaca cgatccatta tcgctgttat aggtacttcc aattcagcaa gcaaacttat
    48961 atgcgaatat ctaaaaacat gagcagtcaa tacaaaatca tctttcttaa tttcactttt
    49021 cctaaacctg tttttcaaaa agtaattgaa acaagttatc attaagggag aaccattatt
    49081 tgtaaatata taatcacctt cttctaaaag aagatattct tttaaaattt ctaacactct
    49141 atcactaata ccaattactc tatatgaagc taaacttttc ggactatcca aaaacacttc
    49201 gttattggac gattacatta tagtaaaacg aaataagaac aggacaaatc gatcaggaca
    49261 gtcaaatcaa tttctaacaa tgttttagaa gtagaggtgt actattatag tttcaatcta
    49321 ctatacaaga cgaaattgaa gaaatctttc cattataaaa cgcatattat caaggttttt
    49381 aaacacctaa taatatgcgt tttttcctat tttaaagact tttatccacc tattatacaa
    49441 agttaagtat ccatcacagt ttacaattag ccttcattac ctaacccatt caccatcctt
    49501 caagagagta acctttaaga accgaataaa taaatggcaa atttcgttca tttaatttaa
    49561 ctgcactgtt aatttgatta gctccatctc ctaaagtatg cactcctact acacggtgac
    49621 tagcatcata aactgtagat ccactagacc cctctaaagt atcaacttgg taatccaaga
    49681 acatgccatc atcacttaaa acttgttttt tatctgtata catttgatga actttaaaat
    49741 tatatgatgg atagcctgtg atagtcacag ttattcctgt caaatttttt tgactagtag
    49801 gaagacccaa agtccctaat tttgcaccaa tgggctcttc tagaattaat aaagccaagt
    49861 catattccct tgcatcctta gaatttaaat ttctaaattc cttcaaataa cgaacttcct
    49921 ttactttgat ctttccaaat ggttcttgac ttggactaac agtcggaaga acataaatat
    49981 catccgcttc tttcccatag tcatgtctgt agtagttgtg agccgctgta attaacacat
    50041 tatctttaat aaaattcgca cttcccctcc cttctacatt cattccatcg gctactccat
    50101 aatacttaga taaaaccata gctgaagtcg aatacggata ctgtaaagta ttatcaattt
    50161 taatcaaatc atcattaccg ataatacttc tgattgcttt tggtagtatg aaccatatgt
    50221 tggtgaaatc tcagataatg aagaatcatt agatcctgga cctttttcta gtgtctcact
    50281 tacctcatat tcttcacccg tactagaaat aacactcaaa gcagatactg tcgataactg
    50341 gctagccaat aaagtactcg caataattga aatacccaat tttttataaa cagttttctt
    50401 cattattgta tcctcctaat gtaattatag cgtactattc taaatttctt aatctactat
    50461 agaatcaaga aatctaccac cttctttaaa taccctccat tatcacataa acaggtaaac
    50521 ttttcaatta atgactgcgc ttttcaatca cgctagaggt acttacttgc ttctttgata
    50581 ctaagttcag ccattctttc cttgtttttc tcaataaagc atgttaccca agtgggattc
    50641 gttttggagt agtctcgcag agtccagcca atggctttat tgataaaaaa ttctgtttgg
    50701 ttcaagttat gaagtagaat cttttccatt aattgagtat tggtcttctc ttttcttaac
    50761 aactggtggt caatagcgac acgtctcagc cagatattat ctgataggct ccattttata
    50821 ctcaatgaaa atcaaagagc aaactaggaa gctatctgca acctcaaaac actgttttga
    50881 ggttgcagat agagctgacg tggtttgaag agattttcga agagtattaa gattatttct
    50941 tctagttcag ggtgttcata caccaaactc cctactactc gatctaggat atctaccgtg
    51001 tcccaccagg attttgtcac gactaactgc tctagcttag gcaaatcggt ttcctttaga
    51061 taagactgca ttgctttcaa atagttagca gccacatatt ggtattttct aggctccttt
    51121 tcccagcaag tgtctgcaaa atcccaatcg ataatctttg tttttttcgc ttctggtaaa
    51181 tatttttata gagtttattt ctttcaggca ccgcaatacc tagaaaagaa aattgatggc
    51241 gcatataggc ttccatgggc cttgcttttt tagagtcttt tgctgcttct agctcctcaa
    51301 gtaaatctgc taaactcatc taaaactcct cttgccccac caaatggtgc tgaaaggcat
    51361 agacagccgc ctgggtacga tcgctgactt caagtttggc aagaatattg gacacgtggg
    51421 tcttgaccgt cttgagagag ataaagaggt catctgcgat gcgctgattt tcgtagccct
    51481 tggcgatgag ttggagaaca tctcgctcac gcgcagtcaa ttcttcatga agttccatat
    51541 gattgcggtg gtattcaacc ttcttgctaa cctcttgctc aatggccagc tcgccagcag
    51601 ctaccttact gacggcatga agcaattcat ctgcactaga agtcttgagc atatagcctt
    51661 tggcaccagc atctaagact ggcatgattt tttcattgtc caaataagag gtcacaatca
    51721 aaatcttggc ttcaggccat tctttaagga ttgctaaggt cgcgtcaatc ccattcatct
    51781 caggcatgac aatatccatg acaatgacat ctggacgcag ttccaaggcc aagtcaatcc
    51841 cttgagaccc gttggacgcc tcacccacaa cttctacatc gtcttggagg tcaaagtagc
    51901 ttttcaagcc caaacggacc atttcatggt catctactag taaaattttc atctttactc
    51961 ctttatcatt ccttatctaa caggggaata cggatatcaa ctgccagccc ttgcttggga
    52021 gctgttaata actgaaccgt ccctgccata tcttcaaccc gctccttgat atttcgcagt
    52081 ccataactca agtcgtctaa gcttcctaac cggaaaccaa tcccattgtc caccaccttc
    52141 agttgcaatt caacatctgt ctgatagagg tagacatcta ggcaagatgc ctgggcatgg
    52201 cggagggtat tgctaatcaa ctcttgcagg atacggaaga tatgctcctc gattttctta
    52261 ggcaatttcg tcatattctg cttgagacta accctaagat cactcttgtc ctcaagctct
    52321 tttaagagaa tctgaatccc ttctatcaag ctcttctgct ccagttcaac tggtcgcaaa
    52381 tgcaagagca aaacccgcaa atccttctgg gcagtttcta aaatagctgt gacactctgc
    52441 aactgggtct gcatcttttc tctatccaat ttcaaagcct gctgactgat acccgataaa
    52501 ataatgtggg ccgcaaacaa ctcctgactg actgtatcgt gcaaatcccg agcaattcgc
    52561 ttccgttcct tctcgataat ttcctcttcc tgatcaaggc tatgattttc agctttttga
    52621 agagcctctg tcaaaaggtt aagtttacct gataaggact tgaaactggc atccaaatct
    52681 ggatctacaa cctgaactac ttcttgccct gccaataaac gcttgagatt agcctgcatt
    52741 tttcttagag aaagctcttc gatccctcgc caaaacaggg ctaagagaca ggtcatggac
    52801 atgctgaaaa ccaacaataa aaagacaaat ttttctgttt tttcgacatc gtacaaaaag
    52861 atagaccagt caaaatcaag tatttccagc aagctgtggg agaaaaaaaa gacaaatagg
    52921 aaggaggtga gagcaataat gacataggct tgttttttca tcctctaacc acctccacat
    52981 caccaatcat agtggtcaag aaaatcttga cactcttgtt actcttgaga tagtcttttg
    53041 tttcttgatg atagtgttca ttgcggaggg ctcgcttggg ctggttgaaa aaagtcaaat
    53101 ccccatagag acagttaacg ctgagactga cttccacatc tacaggtacg atgattttgg
    53161 tcgttcctac catctttctg aggataatga cattgtcatg attggttaag atgactctct
    53221 ccagatgaat agtgtccttg cccatgaagc gaaagagatt gatatcatca aattggcaag
    53281 tctggtagct tgaaaaatga tgaagatttc caaaccaacg atttttctcc ttcttaaccg
    53341 tcacaacctc ttcaaaaacc aaattggtct gctctttttc ctggttcatc atcgggtaaa
    53401 gaagaaagag actatagata accgcaacaa aaatagctag aatcacaaaa ggattgagca
    53461 taacgatgaa aaagaagaga atggttgccg ctactaaaag aagattattc ccctctttac
    53521 cagtatagta gcgaatcaaa agcaaaaaga ggaatagtat cagcagaaaa cgcgaaaaat
    53581 gctctgatac catcaaaatc agagctcctg tcagaagaca ggcttcgata aataaaaaga
    53641 ttttaaattt tctcataggt tcatcctctc ccttctattt tatcacaatt caaaaaagtc
    53701 acctcagtct gaggatggaa aaaaaggcgc tggttacgcc tttttcatct gatcctttgc
    53761 ttcttttaat tttccataaa gaagatagtc tactttttgt agatctgcta tggtggcaca
    53821 gttaagggaa cacataatca agcgtagatc tgctttccag ccttggacaa tgccaatcac
    53881 ttcttcaact gtgtaggttt caaccaattc cagaacggtt cgtgacaatc ccacagcctt
    53941 agcaccaaaa accaagcact taatcatatc cagcggattc cgaacccctc cactaaccaa
    54001 gagttcgacc ttatctttcc attcttgggc attgagaagg gcctgcatgg tagactgacc
    54061 ccattgattg aggtaatcac gctggccact acgacggttt tcgatatagg caaagctggt
    54121 gccaccacga cccgataggt ccactgtacg aacaccgaat tcataggctc tttcgattgt
    54181 cttggcatcc attccaaagc ccacttcctt gaggacaata ggaacgggaa tttgcttgct
    54241 ataatctgct agatgcgatt gccagcttct aaacttcctt tctccctcgg gcatgagtaa
    54301 ttcctgcatg acattgacat gcacttgcaa tagaacagga ttcatctctt ctacagtctg
    54361 aagtcctaac tcgacaggtt tgtccaatcc aatattggtt ccaaggagga gattgggatg
    54421 actagacttg acagaaaaag aatcatccgt tggatttttg agggctgcgc tataagaacc
    54481 cgttacaaat aaaataccac aggattccgc cacctgagcc agcttttgat tgatttctct
    54541 tcccttatta cttccaccag tcatggcatt gatataaaaa ggaaagtccc actttcgacc
    54601 agcaaactct gtcgaaagat cgatttcatc cagattgtaa agaggcaagg aagaatgaat
    54661 cagctccacc tcatcaaagc tattatagga acttttctgc tcaagggcat agaggatatg
    54721 ctcgtcctta cgatttgtcg tcatgtccta tcctttcttg atataagagc tcaatcccca
    54781 gatcggccca acgatttttt aaggttttgg ttgattgcgc atcaaaactc agggcgatgc
    54841 cacagtcacc accaccagca ccactactct tggcaacggc ctgcaaatct tgactggctt
    54901 ctttcaactg tctaagcaaa ggcgtgtaaa tatctgtact caagccttct aaaagcttgc
    54961 tggctacttc tacttgctcg ataatctttt ctgatttccc ctgttccaag gcttcgacca
    55021 aagaaaccac cgtttctttt gaggaactta aaaaattttg attgatattt tgcttgattt
    55081 gctggaccat gtgactcgat acagccactt ccttggtcca tcccactaag aaatcacatt
    55141 ctaaagttgg tttcacttgt gaaattgaaa agccccaatc acgctccaga actgtcgcca
    55201 agttttcttc ttctaaccaa gcagccacct tctggcgatc aaatgactgg tagagaacca
    55261 aatcctctgc cacaatacag gcaaggtcgc ccatggaacc attgtctcct cgcttgagca
    55321 agacagcgct agtcagcttg aacaagagct cctgatcaac agaaacatca tacagagcca
    55381 gtaaagcctt gacaaccaag acaacgacgc tgccactaga acctagacca aactttttcc
    55441 cttctcgttc cattttgcca cagatttcta gggaaaaaga tcttaaattc tgaccgcgaa
    55501 cagcgaggaa gtctcccatc aaagcaatcg tttcttgaat caagctgtag tcaggattgg
    55561 gcgttaagtc cactgcgaaa tcaaacatat ctgaatagat acggtagctg tcagaaaaag
    55621 caatctcagc cctcatatag atgggaatat cctttatcaa agctaactgc cctggctcta
    55681 aaatagcata ttcacctgcc caatagagtt ttccgcaagt tttaacagca atcatcttga
    55741 ctcaaatcct ttgtttttga cacaatcaag cgataacgct gaccgaaaat ttctgacaaa
    55801 tgctccaagt ctttctcctg acaaaagacc ttaacattgg gaccagcatc catggtaaag
    55861 tagcaggcct ctcctttctc acgaagctgg cgaacaaagt ccatagcctc ataagaggca
    55921 tccgtcagat aagaaaaggc tggactagca gtctttgtcg tagcatgcat agccagggca
    55981 tttttctccg ttaattctcc aatcttggca aaatcatttt ccttgagata aatcagcata
    56041 tcctgatagt ccttctcaga ctgacgaacc cagtcgtcaa aagtcgtcga ggtttccaca
    56101 caaagtttca tcccgtcacg gctagagatt ggttttttct tgtcctctag caccaacata
    56161 atcatagcta gtttcaagtc tgtctctaca gagtaaattt ctccactatc cttatcccag
    56221 gctcctagcg gtccataaaa actccgagaa gaagagcctg aggcaaactt ggcttcctgc
    56281 gctaactgac ttctatccaa tccaagcttg aaataagcat tacaagcctt gaccagggcg
    56341 gacaaaccac tagaacttga ggacaggccc gctgcagtag gcatattgtt ttgagtatcg
    56401 atacggacaa agccctcacc agctggacga taacggtcaa taatcttact catcttggca
    56461 tgctcgacct cattttgtag ctgaccattg atgtaaaatt cgtcagctat tacattggct
    56521 ggtaaaggcg acaaggtcgt ctctgtatac atattttcca aagttagaga aatactgcta
    56581 gtagcaggca ccatctcttt ttcttttttc tttccccaat atttgataat agcaatattt
    56641 gcgtaggaac gtactgtcac aggctctcta tccatgtctg aacagctcct ttctcttcta
    56701 atctttctgc tagttcttgt gcgtgtgtca aattggttac caaggctatg atacaacctc
    56761 ctagcccacc accgctcatc ttggcaccca gagcaccatg gctaagagtc gtttcaacca
    56821 aaaagtctgc ctcagggcta ctgactccaa tttcttttaa atgtaaatgc gcttgactga
    56881 ggatttgtcc cagtccttca gcatcttttt gtgaaatcgc aacttctgct tgctgggtta
    56941 attctcccaa ggcatgcaaa aacggtaggg catccttgcc cttattttga accacttgga
    57001 tggcttcacg agtatgacca taaacacccg tatcggcaat caccaaatag gcggataaat
    57061 ccatctcaag ttctgtaaat cctacgttct tgataaagcg aataggttgg tcactgagac
    57121 aggtcttagc atccaaacca ctaggattca tatgggcaat catctcagct cgattgacca
    57181 agatttctag tacatcatga ggcagatcag cctgatagta gtcaaatacc gcacgaatgg
    57241 ccgctatgct gatagccgct gacgaaccca tcccccgttt ctcagggata gccgagtcaa
    57301 tctcacaacg aatgcaggct tctgtgatat ccaaatactc cagtgaggca taaaccgcca
    57361 tggacaaggt atcctcctca taaaggcgcc aaggactctc tgcaggaact accttacagg
    57421 tcacctccac ctccaaaaga ggcagggaaa tggcaggata accgtaaacg accgcatgtt
    57481 cccctattaa aattatctta ctatgtgcct gaccgacacc aacttttttt gtcatttttt
    57541 ccttttacta gacgaaaaaa cgtcttattt ttcatacaag tattaattct ttcctatcta
    57601 ttttattata ttttcacaaa aaaagcgatt gtttccattc acaatcgctt ctttcattat
    57661 tgaacccatt cgccattata gttgacagaa tagccatcta cggtcgtatt cactgccaag
    57721 gcacctgagc tataagcgta gtaccatctg ccattgacct ggaaccaacc tgtcgtcata
    57781 gaacgacgaa agaaactcca taccgttaag taaagaggaa agtcgtgagg gatcatgcgc
    57841 cattgacaac ctgttttagt gacgtacaaa gtctcattaa caagtactcg tttcggccat
    57901 ttataggtgc ggtgtttgga gaaatagggt tcaatcttag cccattcttg atcgtttaaa
    57961 tcagtatcat atgctttgcg tgtcataact ctagtttaac attttttgtg aatacaagtt
    58021 ctaaataatc gaccacgaaa atttcttaag tggaaaacgc cttatgaagt atgctacggg
    58081 aaacttatgc acttaatttg acaattcaag atgtaaaaat atatactata gtagattgaa
    58141 actagaatag tacacctcta cttctaaaac atttttagaa atcgatttga ctgtcctgat
    58201 cgatttgtcc tgttattatt ttattttact ataatatttg acaagttatc ctaaaagtat
    58261 tattatgttg ttgtgttata gattgattga atctaactaa aggatcctat tcaattacta
    58321 gaactatcac atactcaagg tcagctcaca gatgagcaac tattttggtt acaatgtcta
    58381 ctaaatttaa gtcaaacaaa taatttagtc aaaattaaaa aaatagagga acataaatat
    58441 gattacaaaa cagaatgtaa tagtgttcta cgatttttac tagataaaac tgtaaattct
    58501 gaaggaagga tcacttcttc aacagaattt ggaaatttcg taagtaattt atcattccaa
    58561 cacggaatag ctggactact gtttcctcta aataaattgt accccccaga actggattct
    58621 aaaatactct ctatcatcaa gaaggcagtg acaattagaa cgacacacat atgaatatca
    58681 atactcactg ctatttggtg atgcaggcta tctatggtta ctcctacatt tattttctat
    58741 cagtaaaaat caatactatc tacaattagc aaacgtcacc gctaagaaat taatagagaa
    58801 ttatgatact ctagaagaaa tagactttgc attgggaaaa tctggtgtcc tattatcatt
    58861 aataaaatac tatcaattta ccaatgacaa tactcttaaa actttcatcc acaatagtat
    58921 atggggaaat ttatcattat ttcctacaaa gagatacagc caaagaaagc attttagact
    58981 atagctttgc tcatggatat tgtgaaattg catatgcttt atttgcctat tctaaagtct
    59041 tagaaccttc tatgttctat aatgatctcc atacattcca tgctgaatta aaaaaattat
    59101 tagaaaaagt tacttctaat actgaaaatt taggaaattt acaactttct tggtgcgaag
    59161 gaatttccgg aataatctta tatctttgta tgtacgattg tgacggaaac aaagatatta
    59221 ttagtaaata tcaagaattt gtttttaacc atcatctaaa aatgatgaca ggatattgcc
    59281 acggaataac tagcttacta caaaccactg tctacaatca aaacaaatta ctgatgaaaa
    59341 aaatccaaca ggtaatttta gcatgttctg aacgagatga tcacggttta ctgatgtttc
    59401 aaggagatag tggtaaagca gatttgtttg acttcggaat aggaagcagg gggtatattg
    59461 gtgtctatta aataataaat tcccattcga tgtgcaaaca taaggagaaa tgtatgaaat
    59521 tattttggac aaacaacata tatagacagt tgctgctaaa cagctgtttt tcatcattcg
    59581 gcgacagtat tttctacctc gccattatca attatgtggc tcagtacaat ttcgctccgc
    59641 tagcgatttt actgatttcc atttcagaga tggttcccct actatcgcaa ctctttctcg
    59701 ggattctagg agattttcaa gaaaatagag tcaaacacgc actctgtatt gccaaaatca
    59761 aaatcctgct ctacgctatt ttgacagtat ttctcgtctt gtcgcccttt tcattagttt
    59821 cagtcattat gattgtcatc atcaacctca tctctgacac cttgagctac ctgtctgcct
    59881 acatgatgaa cgccctctac atcagtgtaa ttaaggatga cctgcatgat gccatggggt
    59941 tcaggcagtc tctgatgagg gttgtccaca ttgtcgccaa tctggctggc gcattcctta
    60001 tcaatgttat aagtattcaa actatttccc ttatcaacac tctgactttt gtcattgcct
    60061 ttttgggcct gtatgttatt cgacatacct tgtatgaggt tgaaaaaagg attgaaatgt
    60121 cacatacagc actgagtttt aagaaatatt ttcaacatct taaacagtcg ctggctgtgc
    60181 tcctgaggtt aaaagatacc gtcatactac tgtttctgac gaccagtatg attgccatct
    60241 tggatgtgtc ccctcggctg attgccctcc gcttcatcca acagacacta gcacaactga
    60301 gcattgggca actcttcgcc ctgctctcca tcatcatgtc ttgtggagct atccttggca
    60361 atatgaccag cagtaatcta tttaaaaata tccgtttcac gcacctcttg gttttctgtg
    60421 agatttccct attggctcta ataactagta tcctttgtca agcctatatc gtaattttcg
    60481 tgaccagttt catcagttct acgattatcg gcattctcag ccctcgccta caagcagctg
    60541 tctttgccca tatccccagt gacaagatgg ggacggtggg ctctgctctg agcacagtag
    60601 acattctcgc cccgtccctg ctctccctat tagccctatc catagcatcg ggcgtttcgg
    60661 tgcagttagc attgatattt ttgtatctta ttttgattgc tcttatcttt tgtcaatggt
    60721 tagtcaagtt caacactcat aactaacgaa aaagcatgtg tagatttcac atgcttttaa
    60781 tctccccaat cgtcaggtca agtacaacaa agtcacttct ttgattaagc gagtgttcta
    60841 atataattat aagcgccctg tcattaccga acccattcgc cattatagtt gacagaatag
    60901 ccatctacgg tcgtattcac tgccaaagca cctgagctat aagcatagta ccagttgcca
    60961 ttgacctgga accaacctgt cttcatgtct ccattacctg catttaggta gtaccaagtt
    61021 gaaccatctt gataccaacc agttgccata gctcctgatg aacggagata gtaccatttg
    61081 ttcccaaggt tttgccaacc tgttttcata tcgccatttg gctggtctaa ataataccaa
    61141 gtggtacctt cctgatacca gccagtggcc attgctcctg aggaacggag gtagtaccat
    61201 ggagcaggtt tgcttctatc aatgtcaata gtaacatgaa aatcttgagt ccaaccctta
    61261 gcaacttcac caattggtag tggattgtaa gaatcatcac caaaatttcc tagcttattt
    61321 aaataatacc aattgccgtc ttcataaatc cagcctgtct ttacagcata attattatca
    61381 aaataataag ttctcttttc agcagataga ggatattgtt caccgtatac ttttcctgta
    61441 ttttcagacg tttttgcctc tagtacttgt ttatctgttt gttctagcaa agcaccatct
    61501 tgtccaaaat agtaccactt ctcctgaagg ccaatttcga agactggacg attatcaaat
    61561 aaatcatcac gataacctgt tccaggaatt tctaagtatt gccaaccgac aaccatctct
    61621 ccaatatcac caaaatagta agttttaccg tctattttat gccaatagat tgctttatga
    61681 tcatctttta cataatattt tctattatcc ttatcaacaa atcggccacc tgtggtattc
    61741 gcaaataccg tatttgtagc tagcaaacca aagaaaaaga ctgctagtag aacttgcatc
    61801 atttttttca aaagtttcat ttatatatcc tcctaaaacg attgtagcaa agacacgact
    61861 gcatgtcaat atataaaaac tcctcaaaaa agcagttaca caactgcaac tgcttttcta
    61921 ttcttgcatg gtgtaaccaa caccacgcac ggttttaatg tagctttttt gtcctttaac
    61981 atcaagcttg ctccgtagat agcggatata gacatctacg atatttgtct cggtcgcact
    62041 ttcatacttc caaacacttt ccaacaattg ctcacgagtc aatactttct tgcttcccat
    62101 aagtgtcgcc aaaaggtcat actcacggcg ggtcagagca atcatctctt caccacgata
    62161 aaccgtgtga tgttcaacat ctatcctaag attgcggtag gtccttggaa ctttcatcag
    62221 actgcagtgt tgatcaatga agtcccgacc tcggaagatc gccgaaatac gcgctaccag
    62281 attttcgata aagactggct tatagatgta tgaaactgca aaacgctgaa caacttccag
    62341 ctcttcttgc aagtcttccc aatgatctaa aatcatgatg actgaggcag gtttagttcg
    62401 actcaatttt tctgcaaaat cctgagccat catatctccc agattaacgt tcaataaaat
    62461 caaatcatag tctgtctgaa gagccatgga aagggctttt tgcccctcct ctaccagatc
    62521 aacccgatac tgctctttct ggagttccaa acttaaaaaa tgagctagat ttcgttcttt
    62581 ctcaagtaat aaaatccgtt tccccatggc tgacctactt atttttcgtc ataccaagag
    62641 tagtggaagg ttccttcttt gtctttacgt tggtaagtgt gagcaccaaa gtagtcacgt
    62701 tgtgcttgga tcaagttagc cggaaggtca gctgaacggt agctatcaaa gtaagtaata
    62761 gctgctgaga aagttggcac tggcacacca gcttgaaccg caagagctac gatatcacgc
    62821 actgcttgtt gatacttagc agtaacatcc aagaagtact cgtccaaaag aaggttggca
    62881 agatctgcat cgcggttgta agcatctgta atcttttgca agaaacgaga acggatgata
    62941 cagccatcac gccagataga tgcgatatct gcaaatggca agttccagtt gttttcttta
    63001 gaggctacac gcaattgagc aaatccttgt gcgtatgaaa tgatttttga gaagtaaagg
    63061 gcttgacgga ttttttcaat caattcagcc ttgtctcctt caaagttgaa ggcagctggt
    63121 tttggaagca ccttgctagc atgtacacgt tcttctttgt aagttgaaat gtagcgtgca
    63181 aacactgact cagtaatcag tgacaatggt acaccaaggt caagagatga ttggctcgtc
    63241 catttaccag ttcccttgtt acctgcagca tcaaggatgt agtctacgat tggtccatct
    63301 tggtcttcat cgtctttacg gctcaagata tcagctgtga tttcgatcaa gtagctatcc
    63361 aattcaccct tgttccactc agtaaagatt tcagccatat cctctgcaga aaggcctagc
    63421 aagtgttgca tcaagtcata gctttctgcg atcaattgca tatcaccgta ctcgataccg
    63481 ttgtgaacca ttttcacata gtgaccagct ccatcaggac cgatataagt cacacatggt
    63541 ttgccatctt ctggtgcttt agctgagatt tcttcaagaa catccgaaac caattcgtag
    63601 gcctcttttt gtccaccagg catgatagaa ggaccttcaa gggcaccttt ttcaccacca
    63661 gaaactccag taccgataaa gttgatacct gagtttgcca attcttcatt acgacggatg
    63721 gtatctttgt agaaagtgtt tcctccgtca atcaagatat cacctttgtc aaggtgtgga
    63781 agaagggctt ggatagtagc atctgtacca ggtccagctt gaaccatcag catgatacga
    63841 cgaggttttt cgattgagtt tacaaaactt tcaacgtcat agcttggtac aaaattcttt
    63901 tcaggatggc aagcaatcac atcttccgtt ttttctttac tacggttgta gatagcaact
    63961 gtgtaaccac gtgattcaat attaagggca aggttacgac ccattacggc catacctacg
    64021 acaccaaagt tagcttttgt catttgacac tcctcttgtt tcatttgttt tattttatca
    64081 tttttacttt tgaaaagaaa gaattttgta acgactgctt agtagtccaa gtcatccgca
    64141 tgaccagcgc catttccgac aaagtagcct gtcttacagt taagggtaaa gagataggtt
    64201 ccatctggct tatagaggtt gtaataacca ttgccgttaa cgatattgac acgttcaagg
    64261 atatattggt ctccagtgat atagccacgt gaacgtgaag ttgctagaat ttgttccaaa
    64321 acaccatccg caaaatccca ggcagagtta ttactatcat caatagcaga ctgattatag
    64381 gcaacacgac tggcacttct ttgaacagca acccctgcac tcgagagacc catattgact
    64441 tcactgcgac tacttctagt ctcatttgaa ctacttggtt ttggctcact agtcgtattt
    64501 gaacttgctt gacttgaact tgagctgcta gtttgacttg aacttgagct agaagtactt
    64561 gtttgctggc tcttaccaag actgatagcc ttatctagca ctttatcaag ctccgtattt
    64621 ccagttttaa tatccgtaaa tttagcatcc gatttggctt tggcattggt atccaacaca
    64681 ccatccacaa tagctggttt ttcaaattgt gcattgacat cttgaatggc cttgatttgc
    64741 gttactagac tatcatattt agatttggca agcgtatgtt cacgactacc ttccagctta
    64801 tcaagtaaag tcttgagttg actcagttta tcaaactggc tatttttcaa agccgtttta
    64861 ttgctatttg tgtaaaaggc gtcataaagt gtattaaaat catccacatc cgattgattg
    64921 gctgtagttg attgagaagt ttcaatttcc ttggtcgaac gagccacttg acgatagaca
    64981 taataagcac tgacacaaat aattactgat acaagcgcca aaacggtcaa aataaagcct
    65041 tttttacttt tcttttctga gtcctcttga tccagacgag aaaggttttg ctcctcttcc
    65101 acttcttcag tcatttctgt tggattttca tcatctagca atagaggatc caagccttgc
    65161 tcctcatcgt ccagaggcaa gggtagtaag atgtcttcag aagatggaac ttcctccgaa
    65221 gaagcttctg acttctctac agcctcacgc atctcttgaa ttaaatcatc cagactctga
    65281 gtttcaacga actcctcttt tttgtattga cgagtcgcaa acttatccgc ctcaatttca
    65341 tctctgtgtt gcttaacata cttgtccaaa atggaatcct caggcaagac tcctgcttcc
    65401 acttcttcat ttttacgaat agcttgacca actgttagct cttttgcttc atcaaaatca
    65461 aattgcggtt cttgaccttc ttttttatga cgatttcgtc tttttttact catgagtatc
    65521 cctttctatt ttacctcttg ccgtttgaca cgctgaccaa aatattgata tagatccact
    65581 ttcaaggttc cgttgtataa cttacgcttc ttatctgctt ggctaccata cttgctttca
    65641 aaagcttcat cactagtcag gataaatttg ctccaagttt tcagcggtgc aaatacttgc
    65701 cccatctcag catagagctt ggtcactcct gcatcatctg acaaacgttc accataaggc
    65761 ggattggaaa tgattactcc attgatttta tcggaacgta aatcctgcac gcgcatctgc
    65821 ttaaaagtaa tatctcctgc aacaccagct gcctgagcat tggccttagc aatttccacc
    65881 atgcgagcat caatatcaca gcccatgata tccaactcaa gcttacgttc tactttttta
    65941 gccgcttctg tgcgcacttc ttgaatcaag cgatcgctga tccagttcca ttcctcaaat
    66001 gcaaaagagc gacgaagacc tggcgccatc tttctagcaa tcataactgc ctcaatacag
    66061 aaagtccccg aaccacaggt cggatcaatc aaaggcttgt ctggatacca gttagaaagt
    66121 tgtaaaatgg ctgctgccat attttccttg ataggagcgc cacctttttc ggtacgataa
    66181 ccacgtttaa agaggctaga cccggtcgta tcaatcatga cagttgccac atctttgaga
    66241 atagagacct caatcttaaa ctctgggcca ttctccatca gaggaacccc ttctgggcga
    66301 gcatagtgtt tctgcaattt cttgacaaca gctttcttag aaatagcctg aacactgggc
    66361 tcattgtgaa gtttggactt aacacattta gcttttgaaa tcggtaaccg agctccaagt
    66421 ggtaaataat tttcccaatc taaagcgaaa actccctgaa atagctcttc aaaagtctta
    66481 gctgggaacg ttcctacgat aattttgata cgatctgctg cccgaagcca aaggttggtt
    66541 tcgataatag ctctcacgtc tccttgaaaa cgaacacgtc cattttcaac ctgacaatcg
    66601 tagcccaact ctcgcacttc acgaccaacg acagcctcaa gccctgctgc cacagttgca
    66661 attaaattaa attctttttt catgttacag tcttgcaaga cctttctata tgtccacttt
    66721 aaaaaatgag gttgagaaaa tttctcagcc ccaacctata tgcaattttc tataagccat
    66781 gttttgttcc agaagtgact aggaccactt ccttcgataa tcatctgtct accactaaca
    66841 gctctagctg atagcagtca gaatactacg ttcgtttccg catactccat gccccgacca
    66901 aaatttgggt tgctagcttg aggggtttac cgcgttccac tctctctgtt tccagaaaga
    66961 ctccgtcact gtggcacttt caagcctact ctggcctatc caaggactta gccatttcaa
    67021 ctgccgtaac gatttctcgt ccctaggctt atggtttcgc ctagcacaaa cactacaggc
    67081 atcacagcct gtgctagcat ggactttcct catgagaagc aattctcctc acgcgattat
    67141 ccaaaaattg cacatctcaa ataactactt aaaaatctga gttatctaaa atttgtttac
    67201 caaaaacttc tttttccaat ctattcaggc gtttcaaaat atcaaaattc gtcatagaac
    67261 ttgtaattgc cgcttcaagg ggttctgctt gaactggtga aggtttcggt ttacgagtta
    67321 attcttcctt caaatccgca atttcctgac gaagtgactt gaccaaggca gcataggttt
    67381 catagtcctt gatgacatcg tctaaaaact cgtcaacttc tactttatta tagccacgga
    67441 cttcacgtcc aaactcttgt tcaaaaatat ctttcgctga aaaaataata cttgccatgt
    67501 ctctctccat tctcgcttgc tagtattatt atataaaaaa aggcaaacaa aaatcaaggt
    67561 caaagcttca attttcggaa aaattttcag caagttcatt taattgatca aatgttaatc
    67621 tctttataaa atagtcctct tgatttttca tcttttggta aaaataagct agttttgttt
    67681 cattttcttc atcataaaaa agataggaac tagtcgtgtt ttccagcaaa aactgctggt
    67741 aatctcttaa ctgccccttg tgttcatagc gaggataagc atatttgaca aagtctacct
    67801 gcttaaagcg actcagtttc atctgattgc cttcattcca attttcccca tgggtttcaa
    67861 aagcaaaaat ggtggccaac tggaaaccgt attcggtttt catctcctga gcaacctcta
    67921 aaacccaata ttcaaaaccc aaactccctg taaacacaag ccaagacacc ccatctgctg
    67981 ccatagcctc taaatcttta cgtatcgctt ttttaatcaa tttaagacga ggatccttat
    68041 cagaaaataa acccaaatca aaagcagaat agcctaaaac caaagctgta gccattttta
    68101 accctttctg tgcaaattat ggtataataa agtgatgtta ttgtaccaat aaggagaatt
    68161 atgatcaact atccacataa agtttcatca caaaaaagac aaacatctct ttctcaaccc
    68221 aaaaatttcg caaatcgagg aatgtctttt gaaaagatga tcaatgctac caacgactac
    68281 tatttgtctc agggcttggc tgttatacat aagaaaccaa ctcctattca aatcgtacaa
    68341 gtggactatc cacaacgaag tcgtgccaag attgttgaag cctattttcg acaagcttca
    68401 acgacggact attctggcgt ttataatgga tattacatcg actttgaagt caaggaaaca
    68461 aaacaaaaac gtgcgattcc gatgaaaaat tttcatccac atcagattca gcatatggaa
    68521 caagtccttg cccaacaagg aatctgcttt gtccttcttc acttttcttc tcagcaagaa
    68581 acctacttat tgccggcatt cgatttgatt cgcttctatc atcaagataa gggacaaaaa
    68641 tcaatgccac ttgaatatat tcgagaatat ggatatgaaa tcaaggctgg tgccttccct
    68701 caaattcctt atctcaatgt tatcaaagaa catttattag gtggtaaaac aagatgaaca
    68761 aaccaacgat tctgcgccta atcaagtatc tgagcattag cttcttaagc ttggttatcg
    68821 cagccattgt cttaggcgga ggagtttttt tctactacgt tagcaaggct cctagcctat
    68881 ccgagagtaa actagttgca acaacttcta gtaaaatcta cgacaataaa aatcaactca
    68941 ttgctgactt gggttctgaa cgccgcgtca atgcccaagc taatgatatt cccacagatt
    69001 tggttaaagc aatcgtttct atcgaagacc atcgcttctt cgaccacagg gggattgata
    69061 ccatccgtat cctgggagct ttcttgcgca atctgcaaag caattccctc caaggtggat
    69121 caactctcac tcaacagttg attaagttga cttacttttc aacttcgact tccgaccaga
    69181 ctatttctcg taaggctcag gaagcttggt tagcgattca gttagaacaa aaagcaacca
    69241 agcaagaaat cttgacctac tatataaata aggtctacat gtctaatggg aactatggaa
    69301 tgcagacagc agctcaaaac tactatggta aagacctcaa taatttaagt ttacctcagt
    69361 tagccttgct ggctggaatg cctcaggcac caaaccaata tgacccctat tcacatccag
    69421 aagcagccca agaccgccga aacttggtct tatctgaaat gaaaaatcaa ggctacatct
    69481 ctgctgaaca gtatgagaaa gcagtcaata caccaattac tgatggacta caaagtctca
    69541 aatcagcaag taattaccct gcttacatgg ataattacct caaggaagtc atcaatcaag
    69601 ttgaagaaga aacaggctat aacctgctca caactgggat ggatgtctac acaaatgtag
    69661 accaagaagc tcaaaaacat ctgtgggata tttacaatac agacgaatac gttgcctatc
    69721 cagacgatga attgcaagtc gcttctacca ttgttgatgt ttctaacggt aaagtcattg
    69781 cccagctagg agcacgccat cagtcaagta atgtttcctt cggaattaac caagcagtag
    69841 aaacaaaccg cgactgggga tcaactatga aaccgatcac agactatgct cctgccttgg
    69901 agtacggtat ctacgattca actgctacta tcgttcacga tgagccctat aactaccctg
    69961 ggacaaatac ccctgtttat aactgggata ggggctactt tggcaacatc accttgcaat
    70021 acgccctgca acaatcgcga aacgtcccag ccgtggaaac tctaaacaag gtcggactca
    70081 accgcgccaa gactttccta aatggtctcg gaatcgacta cccaagtatt cactactcaa
    70141 atgccatttc aagtaacaca accgaatcag acaaaaaata tggagcaagt agtgaaaaga
    70201 tggctgctgc ttacgctgcc tttgcaaatg gtggaactta ctataaacca atgtatatcc
    70261 ataaagtcgt ctttagtgat gggagtgaaa aagagttctc taatgtcgga actcgtgcca
    70321 tgaaggaaac gacagcctat atgatgaccg acatgatgaa aacagtcttg acttatggaa
    70381 ctggacgaaa tgcctatctt gcttggctcc ctcaggctgg taaaacagga acctctaact
    70441 atacagacga ggaaattgaa aaccacatca agacctctca atttgtagca cctgatgaac
    70501 tatttgctgg ctatacgcgt aaatattcaa tggctgtatg gacaggctat tctaaccgtc
    70561 tgacaccact tgtaggcaat ggccttacgg tcgctgccaa agtttaccgc tctatgatga
    70621 cctacctgtc tgaaggaagc aatccagagg attggaatat accagagggg ctctacagaa
    70681 atggagaatt cgtatttaaa aatggtgctc gttctacgtg gagctcacct gctccacaac
    70741 aacccccatc aactgaaagt tcaagctcat catcagatag ttcaacttca cagtctagct
    70801 caaccactcc aagcacaaat aatagtacga ctaccaatcc taacaataat acgcaacaat
    70861 caaatacaac ccctgatcaa caaaatcaga atcctcaacc agcacaacca taacatttat
    70921 cacccagatt tttctgggtg attttgtcat agcactattg tatttatgtg cttaacaaaa
    70981 gcctgagaag atgtcttctc aggctttttc tgtactataa acctcattgg aaaagataga
    71041 gactgcttcc gtaatcttgt taagaggttt tactaaatac tagtctttct tcgtttttac
    71101 tacaaagaga gcagataggg ctagactaac acttgctagg aagagggctg tgtcagattg
    71161 actctcacct gttgctggca agatttgttc aggtgtttct ggtctagcta tttcttgctt
    71221 ataatctggc gtcttacctt gatcggaaac taaagagcta agtttaaact caggtttctc
    71281 tacagttgga gcatctggtt tcgtagcaga atcttttaat tggttgagat aatctgcaaa
    71341 gtattccacc attctttccg tcaagagatg gttatccgtc gcatatttca tgcttgcctg
    71401 aacgctagct acttcctctt gattgtcttt gtctgttaat ttctgagctt tagccaaagc
    71461 tgcttcatag cctgacaagt caagaggtgt ttctgctacc tgtggacgag taaagatgag
    71521 ttccgctgca gattggtatt tatcgccacc atctccgtaa gtcttagtac ctgtaaggac
    71581 aattttctta gccttaattg tcttaccaaa atcaatgtct tttggcttat tgttatctgg
    71641 ccaatcagtt gcagtaaagg tatgctcctt gccagactca tctgtcacaa caagtttcac
    71701 atcacgcaag ttaccatttg aacctgatcc acgtggaacg taacgaagtc cagtgatttc
    71761 agttggttct ttcaagacca tggttgcagg cttgcctaca tctccaccat tccaagatgt
    71821 atgccataaa ctagataagt ttccatcaaa ggcatttgca agaccttctt gagcctgagc
    71881 aggagctgta agacttgcaa agtcatctgc taccaaagcc gttttcttct gaaccaaagc
    71941 attcttcaag gcttcaatct tggcaatctc tgcacgcgct tcttccacac tgatatcatc
    72001 atcggcctga ctaaggttaa agaccgcctc tttcaaagca tccatagact ctttggtgta
    72061 gttagtcatg gcaaccgttg gcaagtagtt cttcagagca ttttctgtca acatcttacc
    72121 tgttagggta atttcttcga tttgaagatt atccatcatg aagtcgttat aaccacggaa
    72181 gttggcattt ccaccagaat caccacgagt attacttgca ttgccagttg agtagatacc
    72241 tacccaagta tcccctgttt ctgcacctgt cacaaggaag gttgccttct tggctttctt
    72301 agaatctgtc caagtatttg gcaattcatg catttccaag ttgcttgctt gagtgccacg
    72361 acgacctgac tggaattctc ccttaccgac tacaaaagca taggtattgt ctgaacctgc
    72421 ttcgtattca aaggttacac ggtaggtctt acctgcttca aaacggaagt tttgtgggat
    72481 ggtttggtaa accaagttac gacggctcac tagtccattt gtcttgagtg accaatttcc
    72541 ttcgataaca tcatcgactt tcttaccgtt ccaaccacgt tgtgtatatg gatcgtgttt
    72601 ttcagacaag tgagtgcggt tgtcttcgac accttcgaca ccacccacta caaatgggaa
    72661 gattccttga gctacatttt caaagtcttg cttgaaggtt ccttgacctg tatcgtgctt
    72721 gtctccatac atgcttgaat tgttttcaaa ggtacgaatt tcatcaaagt aagttgcttg
    72781 atcaccagct tcacgactca atgtcagagt aacatttgag acgtccgatc ctgttgtaaa
    72841 gaaggcgtac atgttttgga agtaacttgt atcgttaact gtagcattgt tacgacgtgt
    72901 attgtgggcg taggctttta catagttgag ggcgagagac ttattggtat aagtagtcac
    72961 ttctttttca ccagtattca cagtgatact tgccttggca ttactacggt tatcgacacc
    73021 gacataaacg gcatacttgg tatttggttt caagccagtt aatttctgag tgagactaac
    73081 tttttctttg tttccttgaa tacgaagcat atcgtttgcc ccttgagact tgacaatttc
    73141 tgccttagaa gcatcgcctg aaatggtcca atgtttcaag gtaccactgt taaatccttg
    73201 gtcatagatg tgcatgcctt cactccatga catttcagga ttggtttgtt ttgaacgata
    73261 gagaacgtat ggttgatttg ctagaagatc tagggtaatt ttaccatctt ttacagttag
    73321 ttcttgctct tctgtcttac cttggtcagt tagcttgtaa aggtaaacct tgctctttgc
    73381 ccaatcgctt ggaagggtcc aagttgttgc accggcctgc gtattgaagt agtacatctt
    73441 ttccttatca gtagaaagtt tcttaccatt tgcatcccag ttccaaggag tcaagtaagc
    73501 tgaaccatct tggatgacac gtccgttgag agttactgta cgttcgcgat attgtggact
    73561 attgacatca tttgacttac gagttacaac tactttatta ttgtcagcat ctaccaattc
    73621 cactcgcatt tctggagtcc atttataggt actaccgtta tcggtcatag tcaccggtgt
    73681 accattttcc catttactta cagtgaagtg ttggaagtac ttagtcatga cgtcatgggc
    73741 aaataagttg gttacatagc cattgtagtc acttcttccc tgccagcctt caaagtcttt
    73801 catgctgtag ccacctagca gtggatagtt ggctgcacca ccataacttc tgtagtcccc
    73861 aacccaagca tctttttggt ggttacggat aaagcgggtg atggcactgt tgataccttt
    73921 attggtgtag ccaccgtagg tcaagtcagc tgcccagtga tggaaggtag agtcgtactc
    73981 accaccatgg ccccactcga tcgcaaagcg ccagccttgt ttgttaattt ctttagcaag
    74041 aacgtgggta gcccaggcac cgttatcacc tgattgacca ttaccccaaa cgtccacata
    74101 gataaagtcg agaccgtcac caagtttttt cttcaaatct tcccaacgtg ccaaacgacc
    74161 atgagctagg tcataggcag catcaatgtt gataccttga tctagccagt tccaaccata
    74221 gctatagctt ccatctggat tcttacggag aattttttca ttgaagtatt tagactcagg
    74281 ataagtttct gaagcgttaa cgtggatacc tagatgagct ccatatttct tagccttctc
    74341 aattagggtc ttgaagtctt cgacaccacc gatacgctta ccaatatcag catagttcaa
    74401 gtgaccagag tcatggcctt cgctaccata tcctttaagg aaaacacctt gcccaagacc
    74461 atctgtgtgg agattgattt tcttgatacc atccaaggtc ataaggaatg gattttgtgc
    74521 ttgagaacca aagttcatcg cgatacggta agctgtgata tccttaactt tttcccaacc
    74581 ttgaggattg ttcataatgc tacgataagc aatggcacca tcttgccaat cgactttctt
    74641 gtctgcattg gcatcttcag tgataacaac cttagcactt ggaagttcct tcgtgtattc
    74701 tgggaaaaca atgcccttat aagctttttc ccattgccat tcagagctgt ggattcctac
    74761 atagttggca tttccgactg tttctttata agctgtcaaa cgagtccagt cattcgaacc
    74821 accaccatag ctattttgag agttactcca aacaccagca gcaagcttat ctgtagaaac
    74881 aaatccatac atgtaaccct tggctaggtc tttcattgga ttggttacat cgatatgatc
    74941 atctccgctg acatgcgtat tgtttgacat ggttgcccca tcaaacttag caccagtttg
    75001 atcactagaa acagagacta aagcattgcc gaggaaacta atagaagaaa gtagttttct
    75061 ttcgtcatca atcttttgac ctggagtgac ttgattgtgg ttgacaatct tggtcacatc
    75121 aaagtgcaat tgattgtcca caacttgcaa gcgtactgtc atttccgcat tgattaagtg
    75181 agcatcatcg cgaagcttca tcaagtactc tgctgttgtc tcattgattt tcttataagt
    75241 gacttcaggg gtgattcggt ggttattgat aaagacttgg ttgaactgtt gcacctgtcc
    75301 tggcaaagta tgtccattca agctgtattc cttgacacga gggaaggctt ggtcaatcac
    75361 tgctttgagg accttagact ggatcgtatc ataagtcacc ttgctatcgt caacaaccgg
    75421 acctgtttct ttctgggcag gagtatcatc cgcttttacg ccttcttgat tgtctgtttt
    75481 aacgctgaca actgtacggt cattgccata agttcctgcc ttgagaagga tcttcttctc
    75541 atttttaaga ttatcattga ccgccccagg cagtgtcact gtgtcaaaga gattgacatc
    75601 gttattgctg gcattgagtt gtccatctga cttgagagtg atagaaagac ggtttacaga
    75661 acccggttct ggtgcaacta cacggttgcc tttataccat gtgctattac ctggagtttt
    75721 gtattcccag aaccagccat ccttgtcata accgacaaaa acattattat tggtatcttt
    75781 aaatttcagg aagacaccaa agcgtgattt gcccttttca gaatcttctt tgaaggttaa
    75841 atcaacagtt gcatttccat tggcatcaac ggtcaagccc ttcttttcaa acagggctgg
    75901 tttgcctgcg ttatcatttt gagcagttga gaatagttgg ttgtagcgga cacctttttc
    75961 ttctcggata gtgactgttc cctgttgttc ttttttctct accgtttgcc attcaggagt
    76021 taccgtctta ggtgtttcag gtttagcagc tgcaggttta tcctcttttg gtttttcttc
    76081 agccggtttt tctttttcaa gtgctaatag ttcttcttct gtcttaggtc catctgtaac
    76141 ttctggagaa ccttggtctt ctcccacctt aggcgcttca aaatcacgcc catcattctc
    76201 gtttgctgtt gcaagagcag tagctaaatc agctggtaag ttcgccgtgg aaccagactg
    76261 cacgctatct gcaagaaccg gacttgtccc aaagaatgca gctccaatca taacagaagc
    76321 aacacctaat gaaaatttcc gaatactata tttacaacgt ttttcaaata atcctttatt
    76381 cataaacttc taaatccttt cattttttag caagcgttta catttccttt ttaaaaaata
    76441 ctccttcctc tgaaagtgca atgcttaacc ctgataaata aaaaataatt gtacttttaa
    76501 tataacaaat aatagctgag aaagcaatag gaaacaagta aaattaacaa aaattttctc
    76561 tattctgaca aaaagaggct ccaacttttg ggggtcagta catttagaat ctttctttac
    76621 aaatgtaaaa aaggattcag cattcaagag aaatactaaa tcctttctta tattttgcaa
    76681 cagttatttc acatgttttg cgagatcttc ttgagccttt ttattagact cttctttttc
    76741 tttcatccat ttttcctgag ctttttggta ttcatctaca gtgactgcct tgtcttgaag
    76801 ttccaagtat ttatacaaga ctggttcact tgtaccttta tttcctgaca atgcaaatgg
    76861 tattgtaaat ggtaccatct tagacaagat tgggcgccct gtacgagatg tagttggaat
    76921 aatcaaagca ctatctgtca accaagcttg ggctgcagcg tatttatcat agcgtttagc
    76981 aacatctgta gcctcatcac cagcctcagt gaccaatttt tcgtagtcat atagacctac
    77041 ttttttagca gctacattat cttcccctga gtcaaaccct aaatatgttt tagtactttc
    77101 tcctacagaa ggtttgataa tatcaaggta ggttgatgga tcggcaaagt ctggacccca
    77161 accgacatta tctgataaat cccagtcttc gccagcagca ttttcagcaa aatatgtaat
    77221 attgtttact tcgtcttttt gtagttgttg gatatcaata atgacattat cagctcctaa
    77281 agttgcttcc aaggattgtt tcatagattg gacgcgctga acttttgtag ttgctgtctg
    77341 gtcaactggc atatccaaat gaattgggaa agtcacacct tctgcttgta aggctgattt
    77401 agctttagca aattcagcct tggctttttc tggattgtaa agaccatcct gagaatctgc
    77461 aagattaaca tccttccatt catccccata agtgaccaat ttctctttga ccatatcgcc
    77521 aaagttttta ccatctgctt gaacaaatgt tggtggaaca aagatattac gtaagatttt
    77581 gcttgctcca gtttgtccat tcaactgaga ggcataggct gtacgatcaa aaccaaaggc
    77641 aatagcctga cggaaatcct tgtttaagag agccttttta gtcgatgcct tttgttcgtc
    77701 gctggtctta gatgtatatt tataggactg acggtcaata tttgtaccaa ctagatacgt
    77761 aatagagcct tgttgagtat agacaatatt gtccttcata ctcttctcaa gctctgcgaa
    77821 acttgcactt gttggataga gacgagctgc tgtaaggcta ccatctttaa agttttctgc
    77881 aggtttgctg gtatcttgac catcccagaa tgacaattta actttgtcaa catgcacatt
    77941 gtccttatcc cagtagttcg gatttttcgc aaattcaaca gaggattttg tcacaatgga
    78001 tttcaacaaa taaggaccgt tatacaagag actacttgga tccgtcgctt tggcaaaatc
    78061 atcccctttt gagttcaaaa actcttcatt aactggcgca agcacaccca tggttgtctt
    78121 agaattccag aagctttctg gtttgttcaa agtgtactga actgtctgat catccaaagc
    78181 cttaattcct acttgtgaga aatctttgat ttcccctttt acataggcat ccaacccttt
    78241 gattgattct tgaacaaggt aaagagcatc tgatttttta tcagcagcat attttagtcc
    78301 tgttacaaag tcttgagctt tgactgccgc gtattcttca ccttcagaag tataccattt
    78361 tgcatcctta cggatagtat aagtgtaagt caatccatcc ttggatacag accaatcctc
    78421 agccatagac ggcacaaagt tcccgtagcg atcattttct agcaaaccat caaccacgtt
    78481 actggtaata tttgctgtcg cagccttagc agttgtcaaa tagttgaggt tatcagggtc
    78541 tgtctcataa atgtatgaga atgtcttctc acctttagtg cttgaacctg atccagagca
    78601 tgcagctaaa gtagtcgccg ccaataatgt cacgcccgca agggcaagta gttttgaact
    78661 tttcatcctt ttctccttta aaactttctc tccattatag actcttttca gaaagttgtc
    78721 aacagaattt tcagaatttt tgaaaattat ttttcaaata acatctttgc aaaaaatatg
    78781 aatatcgtaa ccg
//
LOCUS       DAVBCB010000003        67315 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVBCB010000003 DAVBCB010000000
VERSION     DAVBCB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67315
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.003"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(134..1882)
                     /locus_tag="VQU28_000157"
     CDS             complement(134..1882)
                     /locus_tag="VQU28_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475591.1"
                     /translation="MKRQTVNQTLKRLVVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKELSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTISRVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            complement(1884..3608)
                     /locus_tag="VQU28_000158"
     CDS             complement(1884..3608)
                     /locus_tag="VQU28_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475592.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFLSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGLNQEVTDQELWQALEIAQAK
                     DFVSDKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENLPNMSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQU28_000159"
     CDS             complement(3666..4604)
                     /gene="msrB"
                     /locus_tag="VQU28_000159"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2475593.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            complement(4746..4823)
                     /locus_tag="VQU28_000160"
                     /pseudo
     CDS             complement(4746..4823)
                     /locus_tag="VQU28_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4899..5768)
                     /locus_tag="VQU28_000161"
     CDS             complement(4899..5768)
                     /locus_tag="VQU28_000161"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HET2475594.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTKGVRVEAK"
     gene            complement(5770..7056)
                     /locus_tag="VQU28_000162"
     CDS             complement(5770..7056)
                     /locus_tag="VQU28_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HET2475595.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQU28_000163"
     CDS             complement(7207..7944)
                     /gene="mecA"
                     /locus_tag="VQU28_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HET2475596.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            complement(8144..9178)
                     /locus_tag="VQU28_000164"
     CDS             complement(8144..9178)
                     /locus_tag="VQU28_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2475597.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            complement(9207..10640)
                     /locus_tag="VQU28_000165"
     CDS             complement(9207..10640)
                     /locus_tag="VQU28_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HET2475598.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMV
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFIAKMLTKEN"
     gene            complement(10656..11642)
                     /locus_tag="VQU28_000166"
     CDS             complement(10656..11642)
                     /locus_tag="VQU28_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2475599.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQIYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            complement(11644..12747)
                     /locus_tag="VQU28_000167"
     CDS             complement(11644..12747)
                     /locus_tag="VQU28_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2475600.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDLEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYASKLQIIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            complement(12737..13582)
                     /locus_tag="VQU28_000168"
     CDS             complement(12737..13582)
                     /locus_tag="VQU28_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2475601.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLTLDVLPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQU28_000169"
     CDS             complement(13861..15135)
                     /gene="psr"
                     /locus_tag="VQU28_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HET2475602.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQU28_000170"
     CDS             complement(15132..15980)
                     /gene="pheA"
                     /locus_tag="VQU28_000170"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HET2475603.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     DYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYIDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            complement(15977..16453)
                     /locus_tag="VQU28_000171"
     CDS             complement(15977..16453)
                     /locus_tag="VQU28_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HET2475604.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQU28_000172"
     CDS             complement(16446..17729)
                     /gene="aroA"
                     /locus_tag="VQU28_000172"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HET2475605.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKEYTRNHTEDMLQQFGGHLSVDGKKITVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIAPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            complement(17821..18159)
                     /locus_tag="VQU28_000173"
     CDS             complement(17821..18159)
                     /locus_tag="VQU28_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HET2475606.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            complement(18170..19273)
                     /locus_tag="VQU28_000174"
     CDS             complement(18170..19273)
                     /locus_tag="VQU28_000174"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HET2475607.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSETILDRIEDFKERLEVIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQU28_000175"
     CDS             complement(19283..20449)
                     /gene="aroC"
                     /locus_tag="VQU28_000175"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HET2475608.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            complement(20459..21526)
                     /locus_tag="VQU28_000176"
     CDS             complement(20459..21526)
                     /locus_tag="VQU28_000176"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HET2475609.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            complement(21545..22399)
                     /locus_tag="VQU28_000177"
     CDS             complement(21545..22399)
                     /locus_tag="VQU28_000177"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HET2475610.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKKMPTEEIW
                     QSLTEKYQ"
     gene            complement(22389..23066)
                     /locus_tag="VQU28_000178"
     CDS             complement(22389..23066)
                     /locus_tag="VQU28_000178"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HET2475611.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            complement(23063..24226)
                     /locus_tag="VQU28_000179"
     CDS             complement(23063..24226)
                     /locus_tag="VQU28_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HET2475612.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRNAYYQDDLTTAFRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDERRFIVMDVFEYF
                     KYAKRKNLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            complement(24911..26545)
                     /locus_tag="VQU28_000180"
                     /pseudo
     CDS             complement(24911..26545)
                     /locus_tag="VQU28_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26526..27275)
                     /locus_tag="VQU28_000181"
     CDS             complement(26526..27275)
                     /locus_tag="VQU28_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475613.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSAPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVEN
                     LRKDHPDQFLESIYLSLAGRKEEVADASQGH"
     gene            27454..28908
                     /locus_tag="VQU28_000182"
     CDS             27454..28908
                     /locus_tag="VQU28_000182"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HET2475614.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKEQGIQPMADVVL
                     NHKAAADNREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQU28_000183"
     CDS             complement(28998..31616)
                     /gene="alaS"
                     /locus_tag="VQU28_000183"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HET2475615.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLISPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNTSQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKIVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDAKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(31638..32123)
                     /locus_tag="VQU28_000184"
     CDS             complement(31638..32123)
                     /locus_tag="VQU28_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HET2475616.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLLDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(32398..32637)
                     /locus_tag="VQU28_000185"
     CDS             complement(32398..32637)
                     /locus_tag="VQU28_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475617.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILEK"
     gene            complement(32890..33960)
                     /locus_tag="VQU28_000186"
     CDS             complement(32890..33960)
                     /locus_tag="VQU28_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2475618.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQDS
                     AYAFINFMLKPENALQNAEYVGYSTPNIPAKELLPEETKEDKAFYPDVETMKHLEIYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(33957..34730)
                     /locus_tag="VQU28_000187"
     CDS             complement(33957..34730)
                     /locus_tag="VQU28_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2475619.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(34727..35533)
                     /locus_tag="VQU28_000188"
     CDS             complement(34727..35533)
                     /locus_tag="VQU28_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2475620.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(35514..36671)
                     /locus_tag="VQU28_000189"
     CDS             complement(35514..36671)
                     /locus_tag="VQU28_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475621.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQU28_000190"
     CDS             complement(36730..37680)
                     /gene="murB"
                     /locus_tag="VQU28_000190"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HET2475622.1"
                     /translation="MSVREKMLEILEGIDIRFQEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESLSVAKMYAGGFTPCKR"
     gene            complement(37792..38544)
                     /locus_tag="VQU28_000191"
     CDS             complement(37792..38544)
                     /locus_tag="VQU28_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HET2475623.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWFEKKR
                     SLEKADALAYGLGHGGLELIFLGLTSLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLTAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQU28_000192"
     CDS             complement(38554..39285)
                     /gene="budA"
                     /locus_tag="VQU28_000192"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HET2475624.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            39427..40698
                     /locus_tag="VQU28_000193"
     CDS             39427..40698
                     /locus_tag="VQU28_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HET2475625.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQLGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(40718..41533)
                     /locus_tag="VQU28_000194"
     CDS             complement(40718..41533)
                     /locus_tag="VQU28_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2475626.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEILSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKAQLTSLTNMGEAVNELQAGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQU28_000195"
     CDS             complement(41690..42343)
                     /gene="phoU"
                     /locus_tag="VQU28_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2475627.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQU28_000196"
     CDS             complement(42355..43113)
                     /gene="pstB"
                     /locus_tag="VQU28_000196"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2475628.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQU28_000197"
     CDS             complement(43126..43929)
                     /gene="pstB"
                     /locus_tag="VQU28_000197"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2475629.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQU28_000198"
     CDS             complement(43940..44824)
                     /gene="pstA"
                     /locus_tag="VQU28_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2475630.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQU28_000199"
     CDS             complement(44814..45731)
                     /gene="pstC"
                     /locus_tag="VQU28_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2475631.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQU28_000200"
     CDS             complement(45731..46609)
                     /gene="pstS"
                     /locus_tag="VQU28_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HET2475632.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(47183..48487)
                     /locus_tag="VQU28_000201"
     CDS             complement(47183..48487)
                     /locus_tag="VQU28_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HET2475633.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEEFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(48488..49261)
                     /locus_tag="VQU28_000202"
     CDS             complement(48488..49261)
                     /locus_tag="VQU28_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HET2475634.1"
                     /translation="MKSKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLASFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRAVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(49251..49529)
                     /locus_tag="VQU28_000203"
     CDS             complement(49251..49529)
                     /locus_tag="VQU28_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HET2475635.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGKGKISLEK"
     gene            complement(49529..49930)
                     /locus_tag="VQU28_000204"
     CDS             complement(49529..49930)
                     /locus_tag="VQU28_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HET2475636.1"
                     /translation="MITLFLSPSCTSCRKAKTWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(50140..50757)
                     /locus_tag="VQU28_000205"
     CDS             complement(50140..50757)
                     /locus_tag="VQU28_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HET2475637.1"
                     /translation="MKTKLTFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYESFFGYLCLKSRRK"
     gene            complement(50747..51520)
                     /locus_tag="VQU28_000206"
     CDS             complement(50747..51520)
                     /locus_tag="VQU28_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HET2475638.1"
                     /translation="MKYKGYLIDLDGTIYKGKDQIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(51531..52268)
                     /locus_tag="VQU28_000207"
     CDS             complement(51531..52268)
                     /locus_tag="VQU28_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HET2475639.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEDYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAGQ
                     ELIHMMATFVLMDRDSRKVHAVEPEIVAPYQSDFDKKLIRGPKYESLEESISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(52273..53403)
                     /locus_tag="VQU28_000208"
     CDS             complement(52273..53403)
                     /locus_tag="VQU28_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HET2475640.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(53487..53876)
                     /locus_tag="VQU28_000209"
     CDS             complement(53487..53876)
                     /locus_tag="VQU28_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HET2475641.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(53892..54275)
                     /locus_tag="VQU28_000210"
     CDS             complement(53892..54275)
                     /locus_tag="VQU28_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HET2475642.1"
                     /translation="MLEVAYILVALALIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(54276..55064)
                     /locus_tag="VQU28_000211"
     CDS             complement(54276..55064)
                     /locus_tag="VQU28_000211"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HET2475643.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(55057..55992)
                     /locus_tag="VQU28_000212"
     CDS             complement(55057..55992)
                     /locus_tag="VQU28_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HET2475644.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLSETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(56122..56298)
                     /locus_tag="VQU28_000213"
     CDS             complement(56122..56298)
                     /locus_tag="VQU28_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HET2475645.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(56438..57145)
                     /locus_tag="VQU28_000214"
     CDS             complement(56438..57145)
                     /locus_tag="VQU28_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HET2475646.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            57296..58324
                     /gene="queA"
                     /locus_tag="VQU28_000215"
     CDS             57296..58324
                     /gene="queA"
                     /locus_tag="VQU28_000215"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HET2475647.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIINMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDSMFIY"
     gene            complement(58995..59384)
                     /locus_tag="VQU28_000216"
     CDS             complement(58995..59384)
                     /locus_tag="VQU28_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2475648.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTDWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(59438..60145)
                     /locus_tag="VQU28_000217"
     CDS             complement(59438..60145)
                     /locus_tag="VQU28_000217"
                     /inference="COORDINATES: protein motif:HMM:NF019142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2475649.1"
                     /translation="MFRRNLNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGGIIVK
                     VATKPKVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTE
                     QVTVDRKQATDTVIKVPAKSKVEEVLVPCATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDDPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(60845..61396)
                     /locus_tag="VQU28_000218"
     CDS             complement(60845..61396)
                     /locus_tag="VQU28_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2475650.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDIEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGNGMMTETLKAILDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQU28_000219"
     CDS             complement(61794..62618)
                     /gene="nadE"
                     /locus_tag="VQU28_000219"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HET2475651.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(62615..64075)
                     /locus_tag="VQU28_000220"
     CDS             complement(62615..64075)
                     /locus_tag="VQU28_000220"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HET2475652.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(64191..64679)
                     /locus_tag="VQU28_000221"
     CDS             complement(64191..64679)
                     /locus_tag="VQU28_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475653.1"
                     /translation="MKNRFYYYQLLDEREEQLINKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(64669..64878)
                     /locus_tag="VQU28_000222"
     CDS             complement(64669..64878)
                     /locus_tag="VQU28_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2475654.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(65268..65710)
                     /locus_tag="VQU28_000223"
                     /pseudo
     CDS             complement(65268..65710)
                     /locus_tag="VQU28_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(66002..66808)
                     /locus_tag="VQU28_000224"
     CDS             complement(66002..66808)
                     /locus_tag="VQU28_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475655.1"
                     /translation="MILKDINFALNKGEIVGLVGRNGVGKSTLMKILVQNNQPTSGNI
                     ISSDNVGYLIEEPKLFLSKTGLENLKYLSNLYGVDYNQERFRSLIQELDLTQSINKKV
                     KTYSLGTKQKLALLLTLVTEPDILILDEPTNGLDIESSQIVLAVLKKLALNENVGILI
                     SSHKLEDIEEICERVLFLENGLLTFQKVGKDSHNFLFEIAFSSATDRDIFITKQEFGD
                     IVQEEGLRITMSGNIQSSELFKFFNENSIKVVDFETKKETLKDIYLNRSK"
     gene            complement(67214..>67315)
                     /locus_tag="VQU28_000225"
     CDS             complement(67214..>67315)
                     /locus_tag="VQU28_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2475656.1"
                     /translation="VPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
BASE COUNT        20052 a        15155 c        12520 g        19588 t
ORIGIN      
        1 acctacggct agcttcctag tttgctcttt gattttcatt gagtattaca gtaaacaatt
       61 tttctagcta ttgtaacaaa aaagtcactc gaaggcaact tttttcgtcc ataaactttc
      121 aaaagagaat ggcttattca gaactaaaag ccgcagcttt ttgcatttgg taatacttac
      181 cctttctatc catgagttct tgatggttac catattcaac aatatcacca tctactaaga
      241 caagaattaa atccgcatcc tgaatggttg acaaacggtg agcaatgatg aagcttgtgc
      301 ggcccttcat gagttttgca aaggcatcct gtaccagcac ttctgtccgt gtatcaatgg
      361 aagaagttgc ctcgtctaag ataagaatct ttggaatagc cagaaagact cgggatatgg
      421 tcaagagctg agcttggccg acagagagag attctccagc attttccaac ttggtatcgt
      481 atccctgtgg caactgttgg atgaaaaagt ctgcattagc tgctttggca gcagcaatta
      541 cttgctctcg actggcttca ggattgccaa aggcaatatt atcatgaatg gtcccttgtg
      601 tgagccaggt ttcttgaagc accataccaa actgctgtct caatgatact cgtgtataat
      661 cataaatgga ttgcccatcc agcaagatat ctcccgagct aatgggataa aaacgcataa
      721 ggagattgat aagagttgat tttccagcac ctgtcggacc aacgatggct accttactac
      781 cagctggaat atcgatagac aactccttaa tcaaaatttt ttcaggatgg tagccaaaag
      841 agacatgttt aaaggaaata gctcccttaa cttggtcact ggtcaagact tccttacctg
      901 tttcagccac ttcagggcta tctaagaccc catagatacg ctctacgcaa gccagagcac
      961 tttgcaactc agctagcact gaagaaatat cgttaaaggg cttggtgtat tgctgaacat
     1021 agttcaaaaa agtcactaaa cgaccgacgg tcaaggttga acccatcatg atacgataag
     1081 ctcctactcc agctaaaagg gcataaatga gtgcatttac aaagcgagtc gaaggattga
     1141 ccgttgaaga ataaaagatg gctgactgag aatagcctga gtagttgtca tgagcctcac
     1201 gcaatctttg gataaattct gtttgagcat tgaaggactg gattatagtc tgctggctaa
     1261 gcgattcttc aatcaactga gtctgaattc ccctcgtctc tgtttgcttc tggaagagat
     1321 gataggatct cttggcaata aagcgtgaaa tcaccatgga cagtggcgtc aacagcaaga
     1381 ctaagagagt catgaggaga tgaatttgga gcatggctag aatactaacc aaaatcatca
     1441 aaacaccaat gaaaaattgg ttaaaaatca tggtcaagcc agctgccaac tgttcgatat
     1501 ccgtggttac acgactgacc atctctccat tgccttgccg atccgcaaaa gcaatcggta
     1561 accgatggag tttatggatg attcgctctc gtaaatctct ggtataagag aagattagac
     1621 gattatagag gagaggattg gcccattgta ccagagtatt tcctattacc accaagagca
     1681 tctggagaaa aatctgccaa aaaactggtg atgaaccagc cactaggact tggtcaatga
     1741 cctgcccaat cagaataggt aggtaaattg ataagccaac ttgggcaata gttcctagaa
     1801 aggctaggaa aaggaggaag ggatggtttg ctaaatctac gactaaacgt ttgagcgtct
     1861 ggtttacagt ttgtcgtttc attctagtcc tcctttccat gttgggatgc attgatttca
     1921 cgatagactt gactggattt catcaagtca tcgtgcttgc caacagctag caactcacct
     1981 ttttccaaga ggagaatctg gtccgccatc tggagtgtcg aagtccgttg agaaatcaag
     2041 attaagctca tatttggcag attttcgcgg atagctttca agagcttgga ctctgtaatg
     2101 gtgtccagtg ctgaggtcgc atcatctagg atgagaaacg gagcctggcg caagactgct
     2161 cgtgcgatag acagcctttg tttttgtcca cctgagaaat ttcgccctcc tgcttcaact
     2221 agagcatcca agagtccttc cttatcactg acaaaatcct tagcttgcgc aatctccaag
     2281 gcttgccaga gttcttgatc agtcacttct tgatttaaac ctaaagtcaa gttggaacga
     2341 atagttccct taaagagttc gaccttttga ggtacatagg caatccaaga ccgccactgc
     2401 tccaaattaa gaggactacg tccattttga taaaggtcaa tgttcccctt gtctactgga
     2461 taaagtccaa gtaagagttg caccaagctt gatttaccag aaccagtccc cccaatgata
     2521 ccaaggattt gtccttgagt catatcaaag gaaatgtctc tcagagaagg ctgggccgca
     2581 tcaggatagg taaaagtcaa ttcttggact tgtaaaaccc gaccactggt aacttgcttt
     2641 tgttctaatt ctgaatggat gttttcggga gattccgcaa agacctcctc gattctcttg
     2701 gctgagatat aggactggtt gagggaattg atcagcatgg ctagcttgac caattccacc
     2761 aaaatctgta agaggtaatt gataagagca atgagagcac cttgactgag tactcctcct
     2821 tgaattgaaa tatagccttg ccagataata acgagaagag ttccattgac aatcagatag
     2881 gtcagaggtg ttaataaact agacaagaaa cctgtctttt cttgtaatct agcataaact
     2941 tggttaaggg tttgaaaaat ctgtaactct cgtttttctt gaccaaaagc acgaataacc
     3001 cgcatccctt gcaattgctg gcgcgtttcc tgaaccagtt ggtccgtttt ctttctgaga
     3061 ctactgtaga gaggattgac caatcgagat aaccctacaa tgacaatggt caaaatggca
     3121 accatgacta agaaccagaa agtcaactca gctgagattc gataagccat aaaaatggca
     3181 ccaaaaacga taatgggcgc tcgtaaaaag agacgcagga attgattgat accagtctga
     3241 atctggtagg tatccgaagt caagcgagta accaaactag aagttgtcag acggtctctg
     3301 ctgtcctttg gcaaggaaag aatatgacga taaagatcgt ttgtcaattc cttagcaaaa
     3361 cctactgctg cctttgctga gtaaaattga gctatcaagg ccactaaaac gccaattact
     3421 gcaaagataa ggagcaggcc aatctgcatc cagagatgac cttgatctcc ctgaggtaaa
     3481 gattggtcaa caatcccagc aatcaccatg ggaaccaaga gctcaaaaac agcttctaac
     3541 agcttgaaca agggggctaa aattgattcc ttgatgtagg gtttgaagta agataatagg
     3601 tgtttcataa atcccttcta ttcatattct agaaatgaag aaagtgggaa gccccactct
     3661 ctgttttatt tgtttaagta aggcaataga tagccatatc ctgctttttc catctcatcc
     3721 ttggccacaa agcgtaaaga agcagaattg atacagtaac ggaggccgcc taactcccgc
     3781 ggtccatctg tgaaaacatg acccaagtga gcactgcctg aacgagaacg aacttcaatt
     3841 cgctccattc catggctcag atccttgtaa taatgaatca actctttgga aatcggacgg
     3901 ctaaaacttg gccaaccaca acctgaagca aacttatcct tggcaaaaaa gagtggctca
     3961 cctgtcgtaa tatctacata aatcccctct tcaaaggttt ggtcataggc attggtaaat
     4021 ggagcctctg tagcagcttc ttgtgtgaca cgataagact cttcagatag actggccttc
     4081 aacacctctt gactaggctt ttcatagttt gctgcatcaa tcaatggctt atcagcatcg
     4141 gtcacatcga tatgacagta acctgaagga ttcttcctga gatagtcttg gtggtagtct
     4201 tcagccagaa tgtagtggcg taattgctcc acttctactg caatctttcg acccagcatg
     4261 cgttcctgct cctgcaccac tgtgtagata gctggcaaat ctgcttcatc ctgataataa
     4321 atcccagttc gatattggcg accacggtca ttcccttgtt gattgataga tagaggatcg
     4381 ataactcgga aataataaag taaaatctct ctgagtgaca cttccttctc atcgtaaatc
     4441 acttggaccg tttctgcatg gtctgtttcc ttgatcaact ggtaattggt cgtttcgact
     4501 tgaccattag cgtagccaac actggtttct agcactccag aaatgcgtga aaaatattcc
     4561 tctaggcccc aaaaacaacc acctgctaga taaatttctg ccatctttat ttctccttta
     4621 tcacgttttt aactaatact cttcgaaaat ctcttcaaac cacgtcagcg tcaccttacc
     4681 gtaggtatgt tactgacttc gtcagttcta tctgcaacct caaaacagtg tcttgagctg
     4741 acttcgtcag ttctatctgc aacctcaaaa cagtgttttg agcagcctgc ggctagcttc
     4801 ctagtttgct ctttgatttt cattgagtat aaacttcttt tcacaaaaag gatatgaaac
     4861 cctctggtca agagtttccc catcctatct tctattcttt attttgcttc tacacggaca
     4921 cctttggtat caactctcaa gtcatgcagt tttcctttga aaggttgctt ttccaattct
     4981 gccttaattg ttggcatctt gtcatgagaa gccagaacca taactgtcgg cccagcacca
     5041 gaaaggtagg ttgcataggc cccattttct ttggtcactt gcttaatcat cgcaaattct
     5101 cttaccaagt cctgacgata gcgctcatgg aagaggtctc cctcgattgc ttgcccagcg
     5161 gtcaccatgt ctcctgccaa caaggcagca accgctacat tggcgataga acttgcagca
     5221 acagcttcct tataagacaa ttttttaggc aagacactac ggctgtcgcg agtacgtaat
     5281 tcatagtttg gaatgtaagc tagaaaatca cactctggaa agtctgctac gatagcagag
     5341 acttgccctt caacagaact tgcaataacg agattaccat aaatggctgg agccacattg
     5401 tcaggatgcc cttcaatctt ggtcgctaac tgcaattttt catggtctga taagttgagt
     5461 tgacccagtt ggttggctag ttcaatccca gcaacgataa ccgagctgga agaacccaaa
     5521 ccgcgcgcca aagggacatc actggtcatt ttcaagcgtc ttggttgcaa gtctggtaca
     5581 atttgcaaag cgattttaag caagagatta cgctcgtcat gtggaatcca tttgccaatc
     5641 tggtgttcaa tcagccactc atctcgttct tcgcagacct caatttgaag atacttggtt
     5701 acagctacac cgaccgagtc aaaacctggc ccgatattgg cactggttgc aggtacaata
     5761 atcttcatct tattctccta gcaccttgaa ggtattcaag aggtcgaatt ctgaaacctt
     5821 cttcaattca gctgagacat tttcaagctg ggctttatta atcttgtgtg tgatgataac
     5881 gacacgcgcc ttgtcaccct ctttgccatc ttgaaggatt tgcttaaagg aaatatcttg
     5941 agcattgaag atttcagcca acttcaagac ctgacctttt gagtctagag ccaagattga
     6001 gaaatagtag tttgctttga catcttcagg atttgccaag accaagtcac ggctatattc
     6061 gttgaagtct ttgccaatag taccatcatt caaacgacga acgatacgga caatatcagc
     6121 tacaacactt gttgcagttg gtttttgacc cgcacctggt ccgtagtaca tagactcacc
     6181 aataccgata gattctacaa agacagcgtt cattacgcca ttcacactag caagtgggtg
     6241 cgctttaggt aggaaggttg gagtcacttc tgcagcaata cctgaagaag tttcctcaat
     6301 agaaccaacc aatttcacta cgtaaccaag ctcttgagct acagctacgt cttctggtgt
     6361 gatattgcgg attcccttgt gggctacatc atcaaaggca atcttcatgc caaaggcaaa
     6421 ttggctcaaa ataaccatct tgtaggctgc atcaatccca tctacgtcat tcgtcggatc
     6481 gctttctgca aatcctagac gttgtgcttc cgcaagagca tcatcgtaag accagccttc
     6541 ttccaccatc ttggtcacca tgaagttgga agttccgttg actactccaa gcacgcgcgt
     6601 aattttatca gaagccaagg aatttgctaa agtacgaaga attggaatcc caccagcaac
     6661 tgctgcttcg tagtaaagtg ctaccttgtt agcttgagcg atttctagca attctgcgcc
     6721 atggacagct aaaaggtcct tgttagcagt aacaacgtgt tttccagctt ccaaggcacg
     6781 agtgataaag gttttagcag gctcaatacg ccccatcaat tccactacga tagtaatatc
     6841 ctggtctgat aaaatatcat ccacattggt tacaaagtta aagtcattcc ctgctgcaag
     6901 caagcgattt ttttcatctt catccttgac caatacctta gcaactttga tatctgaatg
     6961 tgctgattga ttgatttttc ctccattttc ctttaggagg aaaggcacac cacttgcaac
     7021 ggtaccaaat cctagtaaag caattttaac tgtcatcttt atctcctcga aattttctaa
     7081 tatagccatt ataacagaat tttgtgaaaa ttcctattat agtaaatcac tatttcagta
     7141 taaaaagaaa aaacgaatca gacgattcgc tcttcttaaa atctgaaaat agctttccag
     7201 aaaagattag ccgatttttt gcagattgag cactgcatcg tgactcatca agacttgacc
     7261 atactcttgt aagactgagc gactgatatc actatcgtct gcaaactcgc gcatacgggc
     7321 caacagccaa gctggatatg ggcttggatg attttcaata tccactaaaa tggtcaaata
     7381 atagcgctcg ttcattttgt agagttcaga agtttccatt tcaaaagtca ctgtcttggc
     7441 aaaagctacc aagtcagcca acttagcaaa agaaaggatg tagtagatgt aaggttcttt
     7501 cttactctca gcttcttgtt cagcctgctc ttgctcttct tccttggctt caacttgctc
     7561 aagagattga atggcttcga tatcatcctt ggttttgtct gcgatgcttt tttccagggt
     7621 tttgataaat tcatctggag acatttgagc caattcttcc atatctggca aatccgataa
     7681 gtcttcaaaa tctagatttt ggtcaatctt tgacttggtc acaaagacat ctaccttatc
     7741 aggttttgga gtcacacgga agctcaacat gcctgtatcc agaaagctat caggcatctc
     7801 tagctcatcc aagatagcat aaaagaactc ttctgttttt tcttgaggaa cgagaaagtc
     7861 agcaatctcc attccacgat ccatcaaatc ctctaaagac atcgtgattt ttaaagttgt
     7921 atcactaatt tgtttcattt tcattgctag taacctcata ctttcagttc tatctattat
     7981 actagatttt tacgatttta tcaaaagaag gctcctctat acggatagat tttccctagg
     8041 gtctttctat aggagactcc aagagaaaat ttctgcagac agatagaaaa agccttcaaa
     8101 atcggctaag agccgatttt gaagacctta tacatcagaa tacttataat ttaaaggttg
     8161 ctacaccgag gatagaacga tttaagtttc tgagaatttg aagactttgc tcaaatttct
     8221 tataacgagt cactccgtac tcttcaacaa gaaggactgt atctctttcc aaaagagatg
     8281 atacatcctg taaatctaca aaatgcattc cttttaaagc ttcttgactc tgtttcaatt
     8341 tatctaggat agctttattt gagctaacga tggtcaattc ctgtccagta tttttgtatg
     8401 acaaaacatc tgctaggtta gcaattgttg taatctctgt tacaaaatca atttgatact
     8461 gagaaaaatc acctactcta ttgattgttg gattaaagag ataaactaac acatttccca
     8521 tcacaaccaa aatcacacaa accactccaa taacaactaa acgaagaatc agatttttca
     8581 catttaagcc aagcgctgtt tcaccatttg cgttcaattc tttagagttg atggtttcca
     8641 gtttttcaat tttcacattt gcataggcat gtttaaattt ctcaatcaac ccatcaattt
     8701 ttttctctaa caagtcattg gcatctttac ttgatgtcaa aattttcaca ccaacccctg
     8761 catcgtcaat catatagtag acggtcaatt ttttccacca atagtcattc gttgaatttt
     8821 tcaaggttgt ttctgtcgtg tctaattcac tggcaatttt tttcaactca ctgggttcta
     8881 catcattgaa aagataagct ccattcaaat taccatcaat caatttccca taaaaatcac
     8941 tataaccacc aatttgatga ttcaaaatcg ttttggccga ctcttttgga ggagtgattt
     9001 tatagataag ataagttgaa taacttgttg tatctttgac agtgttttta ttcctaactg
     9061 ctttaattgt aaatggtaca gcaatgagag caaataaagc gatgagagct aaaatatttg
     9121 cttttcgctt tttataaaga tttgcaaaca aatcagctac tgaataatgt tcaaacatga
     9181 tttttttctc ctttgtttag tagatactag ttttcctttg taagcatttt tgctataaat
     9241 ataatcacaa gaacaattcc ccagaattgc attgtaaata aattgaagaa actttctgaa
     9301 aagctgcttc ttggcataaa gaatagatta ttcaagatga gtagggataa agcaaatagg
     9361 attgtccttg agcgataggc tacttgcagc atggctataa ataatacgcc gagtaagaaa
     9421 ctaagcagaa agactccaat cataccatag tcggtataca actccatgat ataactactt
     9481 ccgataccat gccctttcag gtattccttg ttcaagacaa gataggatag attgtgggca
     9541 taactattac tatcaatagc tagttccaca ctattggttg tatgttcaaa ggcttttcct
     9601 ccgaaaatgg ctcccaaact cccccttgca aaataatcaa gaacaggacc aaaagtaaaa
     9661 ttacggaaat ctcggtaagg gaggctactg ttaaatagaa aacctcgagc cagaacacca
     9721 aaactagtcc cttgtttata gataaagtca agtaagatat cccagaaacc tgtatgggaa
     9781 acttggacat tatcccgtac ataattgagt actcccatcg ctaacatgag aataggagaa
     9841 cctacaaaaa tcgctaactt ttctttaaac ccaatccatt ttcctttttc agtttgctcc
     9901 cgcataaagt aataaacaaa agcaaataaa atacttaaaa taaagggatt tcgtgtccca
     9961 attgccaaat gaatagtatt agctgcaata aaggagacaa gcactgctgt ggcctgcaat
    10021 ttctttggct tggttgccag atacatacac attgcataga ccgtaaaggt agacaaaatg
    10081 taggtaaaat aaggcagttt actttcaaaa tttgcatagt aggcatagta ggaagtctgc
    10141 aaacgataca agagccgttc aaataaccga atgaaataga aaggataagt tagaagaaaa
    10201 actcctagtg atacaaagcg taaccgcttg atataaacct cttttagaga atttcctata
    10261 tttgctactt ttattttctt cctagctatg aagtaacgag ccagaatgcc tcctgtggtc
    10321 aagcccagaa tcgaaaccat gacaactata aaggcaaaac gataggctat tggatgatag
    10381 gtatccaaag caccatccct aaaataatca atggtcggtc ttgataccag aaatacaaaa
    10441 atggttaaat agaaaataaa atggattaag taatacttga tatcattcca acaagcaatt
    10501 aagctactaa ccaacaagaa caataaagta gaaagtaagc taacattatt attattaaac
    10561 agatacacaa ttccacttac tagcgtcaag gcataactga ctatggtcaa actaaataat
    10621 aatcgtttcc catcaatcac ttggtcaccc ccgttctaat gtaatttttt agatttttca
    10681 atatttttca gtaataagaa tcgatataag gaaatattta tgaatagggc caaagcacta
    10741 attcttctcc ccttacggaa aattggattc ctagaaatag caaaggcatg gccttttaaa
    10801 aaacgatgaa tctgagaata ggcttcaaac tgtttatact gatcatctag caacatctta
    10861 tccagaataa agaagtgggc ataggccaat ctgaaaaaag cgacctcttt caagtcagga
    10921 tagtttttca caacttcatt ataaaacttt tggtagatat caatataggc taaatccttc
    10981 tctgcatagg gtttggtcgt aatactatcc cctctatgga aatagtaata atagggttta
    11041 gtattaacca catacttctt ggccaacttg attaaatcaa aatggtaata ggcatcttcg
    11101 taaatcaacc ccttaggaaa ggatagggca gttgcaatct gtctcttgat tagcttattg
    11161 caaatcgtcc caggtatttt ttcacctatg aggtattcct ttagaaatgt ttgagaatca
    11221 cagacaaaat agtcatcctg attggctgac tgtgggcttt catcattagc atagacattc
    11281 atgacaccac agcttgaaac atccgcatct tcttgaacta attgctcata taagctctga
    11341 atcatttctg gatggatata atcatctgag tcaataaaaa tcagataatc cccgtgagcc
    11401 tgcttcatcc catcatttcg tgcttgcgac aatccttcgt tctttttatg aagcactgac
    11461 accctgtcat cttgttcagc gattgagtca cacaagcgac cactttcatc tgttgcacca
    11521 tcatcaacaa gaataatttc cagattttga tagatctgct tctgaatgga agctatcgat
    11581 ttttctaggt actgcgccac attatagact ggcacaatca cactaattaa tgcagtttcc
    11641 atgctactcc tctaatagtt tttctacttg ttcgatttgt tttgtaattg taaattgttg
    11701 aatgaattgg ctagcctcat cgacatcaaa gtttgaggca gaagtcatgt aattagtaat
    11761 cgcctgagct gcctcttgat tgctctcaat gatttgtcca aatcgtcctt cttgggataa
    11821 ttcctcagcc cctccaacgt ccgtagagat aaaagggagt cccagactca aggcctccac
    11881 atacactcca ggaaaacctt cttgtttaga catagacaag agaactttcg tctgagataa
    11941 atactgataa ggattttttt gataaccaag gaaatgtaca tagtcctcaa tcccatactc
    12001 tttgactcgt tttttcagtt cctcttccat atcaccagcc ccgataaaat agagatgata
    12061 gttttttccc tcttggtgta ataatcgtat cacttccact acacggtcag aacccttatt
    12121 ttcctcaatc cgtccgatag tacagatact ttgaggagca atctcgatat cgatcttctc
    12181 ttgagatttt tctagaatag tctgaaaatc atatccattg tagattatct gtaatttaga
    12241 agcataatct ggataaactt ccttgataga attgctggtc ttttttgaaa tccctacaat
    12301 tgtattcgca gcatccaact ggcttctatg tgattctctt ttagagctat ccttaagaag
    12361 ttcttcaata cttccatgaa tccaagatat cttcttgact tctcttcttt tagagaacaa
    12421 cagtggtgga ttcataatgg taaaagaaac ttcaacatca taatcatctt ttacaagcaa
    12481 acgacgagtc agtcttggaa aataaattct cattctccac aaaaaagctc gtaaccatct
    12541 ggtttggcga taatcttgaa gggattttaa aatgcgtaca tgctttggaa cagattcata
    12601 tcccttgtca aagtgctcca tttcaagaat atcaatatca tacttttcta gatccagatt
    12661 tgaaacaatg gttgatagaa tcttctctgc accacctcca agagaaaaag accacataaa
    12721 aaataagatt tttttcttag ccaccatatt ctcccttgta ttctgtataa gacttatcca
    12781 tatcagcgat gacagcatca tgatgcggta cctgcttgtc tgctggtgga ggcgtcatat
    12841 aatccccaaa agcagttctg agatagacat catagccgat tggaataggc atctctgttc
    12901 cttcaaatgg caagaaaaga ttgtcttcaa aagatgtgat tgggtacttg tttctcatgt
    12961 agccaggacc tgagcataat tctgtaatgc catcacaatc agccaaatca tacttagtca
    13021 tttctttctc agcttttttc cagatgcgat aacggagaga ttttggagtc aaacccagta
    13081 aaatgcgact tccccatttc atgagagcac catgcttttc tggaatagtt tgcgcacaaa
    13141 agagtgaata aatcaaggcc caacgaacct gttttttccg ctcagctgga tttttcggat
    13201 aataatccaa aggcaaaaca tccaaggtca gaccatgtgg caaatctaaa tcctgctgat
    13261 aaggcttgat acaggtggtt tccttgtcac gaatggtaat aaaaagatta cgatcaacaa
    13321 aatccttgtg actctttgac aagaaataac gttcatctgc ataacgaggc cataattctg
    13381 ctaatttctc ataatcttta cgaggcataa aaaagtctag gtcgtcgtcc caaggaataa
    13441 atcccttgtt tcgaagggca ccaatagcgc ctccgccaca gagataacag agcaaatcat
    13501 gttctttaca aaaggccaca aaatattcag ccatctccag actacgagcc tgaattgctt
    13561 ttaaatcagt catattgttc attattcttt ctatcgtatc gtttcattat accacaaaca
    13621 aggggtgaaa atctattgca gactgtaaaa aatcaaagcc tgactgctat ccaaatagct
    13681 atcaaacttt gatttttctg tcttatactc ttcgaaaatc tcttcaaacc acgtcagctt
    13741 caccttgccg taggtatagg taactgactt cgtcagtctt atctacaacc tcaaaactgt
    13801 gtttttagca gcctgcggct agcttcctag tttgcacttt aattttcatt gagtattatc
    13861 ttatctcaag cccatttgag cgagcttggt ttgatatttg ttttgatcaa ccagcaagcc
    13921 caagcctcca taaacatcat aggcatctac ccagtcaccc agttctggaa tcgtcaattt
    13981 ttcaatacca ttttttgctc catccaaaac agataaaccg tttgttagga ggaaagtata
    14041 gggtacgttg gttgaggtca tagcaaaaac ctttccaaga gcttcagaac cagtgaaaag
    14101 tttagtggga tctttaattt gctctaaaat tgctgttaaa acttgttgct gtctttttgt
    14161 acggccgtaa tccgcctcat catcatcacg gaaacgagca taattgagca gggtcgagcc
    14221 attcatctgc tgttttccga ctttaatggt ttgggttgga gactcagtct cggtagcgta
    14281 taaatcatct ccgactgtag cttctgttag gggacgccca ttcaatgttg aaaattgagc
    14341 atcaatcgtc accccatcag ggaaaagcgt gtcaatcgct gtggcaaagg cctggaaatc
    14401 aaccaaggcg tagtacttaa tgtccaagtc aaaattatct ttcaagactt ggcgaaccat
    14461 ttctgcccct ttttgcccct cttgttctcc taactcgtag gctacgttta acttgttatc
    14521 tgtctgtttt ctaccattaa tcacttgact ataaccatct atatagacca aattatcacg
    14581 catgaaactg actagcttca ttttcttatc tgagcccccg acatttaata ccataataga
    14641 gtcagttcgt gtctcaacac tgttctggcc gattcgacca tcagtaccca tgattaaaat
    14701 attaactcca tctctagtgt cctgaccatt aaagacttct acttgagctg cccgggcatc
    14761 agcagttttc tttgcgctag catcttggta accacgcaaa aacatgaata ccatggccaa
    14821 agccacacag accaaaagtg aaaaaatcac cataaaaatt cgtttaagac ggagcttccg
    14881 tcttttcttt tttggaggga aagagagtgc ttgtgatttg gattgtgagc gactccggtt
    14941 cgcatagctt ggtaagtcaa cctgctcttc tctttcttgt tccaagctag agctactatt
    15001 tcccctagca agagttagct tttcttgcaa ataggcaaac tcattttttt ctctctcatt
    15061 gagatagtga atatttttta gcaaataatc ataacgcaac tgctcatgat gacttaaggg
    15121 attttcttta ctcatcttct ctcctttcca tggtctgata ttggataaat aggataggca
    15181 cccagaattt tatactggat tccaatcgct tctaattctt tttgggcaaa gtggaccaag
    15241 tccttatcga tataatccac atcgataatg aaaaagtatt cacccagtgc tgtcttgagt
    15301 ggacgacttt caatttttgt caagtcaatt cctcgccaag caaaggtcga cagggcctta
    15361 taaagtgcac ctggaaggtt gtcaggtaat gtcaaggcca aactcatctt ttcagtttgt
    15421 gcttgcaagg gaatactagg cttttcagct cctagaaccc agaaacgtgt gaaattggct
    15481 tccatttcct gaatatcctc ggcaatcagt tccaatccat attcttcagc agaacttcta
    15541 ggtgcaactg ctgcaaaggg ctggtctgga tgttcggaaa taaaacgggc cgcataagct
    15601 gtactagctg ttacctcgat ttgagcctct ggatattgtt catcgatgaa tttctttcct
    15661 tgagccaagg cctgtggatg tgaaaaaatc ttttcaatct tagtatggcc tggaaccacc
    15721 atcaactgct gatgaatagg ctgaacgatt tctgctactg cttggatgtg agcctgatga
    15781 aaaagatagt ccaaggtttc atgaacacta ccctcaatag aattttcaac tggcaccaca
    15841 gaatagtcca ctaatccttg ctcataagcc ttgatgacat ctgtaatgtt ggcaaaagcc
    15901 tgcaattcct catgaggaaa agctgtctgc acaacgtggt gtgaaaatga tcccttggga
    15961 cctagataag ctattttcat cttagttcct ctataatttc ctctgggctt agcttggtca
    16021 catccaaaac ccgactagcc acttcctcat accaagcctg tctttcttga aaaatagcta
    16081 ctagttcttc cttgctatta tttagaaaaa gcggtcgctg attgtcctta tcagctgcga
    16141 tacgtaggta gagggtttca aaatctgctt tcaggtagat gttatctgta ttagtcttga
    16201 gtaagtcacg atttctctga gaaataacca ctcctcctcc agttgacacg acttggtctg
    16261 tttgtagtaa atcagctagg acttctgatt ctacatgacg aaaggctgct tctccctttt
    16321 cagcgaaaaa attcgcaatg gacataccta ggcgcttctc aatcagagca tccatatcaa
    16381 ggtaattagg gtccaagcct cttgcaatag tcgattttcc agcccccata aaccctaata
    16441 acaccttagc catgaatcaa gctctccaaa tcatcaaaga aactaggata gctggtattg
    16501 atggcttctg cacggtcaag ctccacctct ccatctgcaa ccaagagggc tgcgatagct
    16561 gtcatcatgc cgatacggtg gtcaccaaac gtattgactc tagcaccgtg aagagctgat
    16621 ttccctttga taatcatccc atctgccgta ggagtaatat ctgctcccat actatttaag
    16681 gcgtctgcca caacctgaat acggtctgtt tccttgacct tgagctcctc agcatccttg
    16741 ataactgtta caccttgggc ttgagtcgca agcagggcaa taatgggcaa ttcatcaatc
    16801 aatcgtggaa tcaaagcgcc accaatctct gttcctttca agtcagaaga ctcaacaatc
    16861 aaggtagcag atttagcgac tggatcgatt tcagttattt ccaattttcc acccatggca
    16921 cgaatgacat caataatacc ggtgcgagtt tcgttgatcc ccacattctg cagcactaga
    16981 cgagaatttg gagcaatcaa acctgcgact aaccaaaagg ctgcactgga aatatctcct
    17041 ggtacgacca ccttctgtcc tgtcaatttt tgtggcccct ggactgtgat tttcttacca
    17101 tccacactta aatgaccacc aaattgttgc aacatatctt cagtatgatt acgggtgtac
    17161 tctttttcga taataactga ctccccctta gcttgtaagg ctgcaaacat caaggctgac
    17221 ttgacttggg cagaggcaat tggcaactca taatgaatag gtcttaggtt tttcgtccct
    17281 tttaagcgaa ggggaggcaa gtctcgttca gtttgccctg aaatgctgac gcccattttt
    17341 ttcagtggaa gggtcacacg gtccatagga cgtttggaaa gactatcatc tccaaacatc
    17401 tctacttcga aatctgcacc agcaaggaca cctgaaatca ggcgaatcga ggtgccagaa
    17461 tttcccatat taagggcatt ttgtggcgct tttaagccag ccatgcctac accttgaatg
    17521 gtaataaccc catctttatc ctcaatttca acaccaaggt cacgaaaaac ctgcatggtc
    17581 gaaagaacgt cttcacctcg cagaatatca taaaccttgg tctcaccctc agccaaactt
    17641 ccaaagataa tggaacggtg gctgatagac ttgtcacctg ggacgcggat actaccatgt
    17701 aaatggcgaa tgtttgtttt tagtttcata ctggacctca tacttgcaat acttttacct
    17761 attttatcat aaaaagtcag aaattcctta aaaattcctg actttaggat cgttcttttc
    17821 ttatttcagc aattctgaaa ctggttcaaa aacaattttt tcaatgtcag aaaggtaaat
    17881 ggccaattgt tgttgcttgg taaagaattc tgacaagagg ctatttcctt gaatctgttt
    17941 accaaagcct tccatcttag cttggaagga cgcatctggc atttgacctg tctgtgctag
    18001 tttttgaatt tcctcttgaa aggcaagata ttctgtaaag attttgcttg cctcagcatc
    18061 tgctgcaatc gcatctttag ctgctttaac agccttgtat tctggtaatc cgcgtagacc
    18121 gcgactgagt tcgtttgcac tatcgtaaat atttgacatg ttcttctcct tatttgatga
    18181 cgactgtata gtcggtattt tctgttatga gatgctcagc tctttccaag tcttgagcat
    18241 ttttaaatga aatttgtagg attccgtgaa tatcctcacg attttcctcg ttgatgtgga
    18301 tgttaaccaa ggaagttcca cgtagcagtt ccaaaatccg caggatgaca tcttcttcat
    18361 caggaacgtc aacatagagg tcgtaagagc tatccacacc accacgctta tggatttcca
    18421 tggtctggcg ttgttcacgc gcttggttaa aaaagttcca aatttgctct tcatctccct
    18481 tactaatggc ctgaccaatc acttccaaac gttccttgaa atcctcaatt ctatccagaa
    18541 tggtctcgct attggacaag agaatggagg tccacattcc tggctcgctt tccgcaattc
    18601 gggtcatatc tcgaaaacca cctgccgcaa agcgccttgc catctcatgc tcttgagcat
    18661 agaccgcagt ctgctccatg agactagaag ccaaaatatg aggaaaatgg ctaatctgag
    18721 aagtgacacg atcatgctcc ttggcatcaa tctcgataaa acgagcatga agacctgaaa
    18781 gcagatcctt catttcctta agcgtgtcct gacttgtcag gcttgaaggt gtaaagatat
    18841 aataggcatt ttcaaaaaga ttgacatctg ccgaagcagc ccctgtcttg tgactaccag
    18901 ccatgggatg ggccccgaca aagcgaacag acttgccagc caaatactgc tccgccgcat
    18961 ccacaatggt tgacttggtc gaaccagcat ctgaaataat aacgccttct cgcaaatcca
    19021 aattggccaa ctccttaatg aaagcaatag tttgtttgat tggcaagctg aggataatga
    19081 catctgccaa aggagcaaaa ctagcaaaat catccgttgc acggtcaatc ataccttctt
    19141 tcaaggcgat atctctcgaa gcttgactac gattataacc taaaatttca taatctggat
    19201 gatcgcgttt gataccaagt gccatagagg ctccaatcaa cccaagacct gcgatataga
    19261 ttgtttttgc cataggaact ccttaatagt tctttgtata gtctcggtgt ttggctaccg
    19321 cttcttttag ttcctcaaga ttatctgatg agaatttttc gaggatttct tgcgccagaa
    19381 ccgttgctac aactgcttcc atgaccattc ctgcagctgg aagagcagtc ggatcacttc
    19441 tctccacggt tgccttgtaa ggttcgtggg tttcgatatc cacactcata agaggtttat
    19501 aaagagtagg aatgggtttc atgaccccac gaacaacgat gggttgccca ttagtcatac
    19561 caccttcaaa accacctaga ttattggtac ggcgagtata accgtcttct ttagaccaga
    19621 gaatttcatc cataacttgg ctgcctttac gataaccagc ctcaaagcca agaccaaatt
    19681 ccaccccttt aaaggcattg atagagacaa cagcttgagc caatcttgca tccaattttc
    19741 tatcccattg gacataggaa ccaagaccaa ctggaacgcc tccgacgact gtctccacaa
    19801 ccccaccgat ggtatcacca tcacgtttga tttggtcaat atagtccttg atttcctgtt
    19861 ctcgttcttg gttgacaata gaaacttcag actgggcagc tctttgctta atttcagcga
    19921 ctgtcagatt ttcaggaaca tctatttcct taccaccaaa gaccacgaca tggttggcaa
    19981 tctccatatc cagctcagcc aagaggcgtt tggctactgc accaactgcc acccgcatgg
    20041 tggtttcacg agctgatgaa cgctccaaag aatttcgcaa atcatcaaaa cggtacttaa
    20101 tccccccaac caaatcggca tgacctgggc gaggatgagt aattttccgc ttgcttttaa
    20161 ggcggtcttc aatgtcctcc gcagacatga tgtccagcca tttctggtgg tccttattga
    20221 tgacatccat agtaataggc gcccctgtcg tcttcccgtg gcgaacaccc gaagtaaaga
    20281 caacctggtc actctcaatc ttcatacgac caccacgacc gtagccaccc tgacggcgtc
    20341 taagatcctc attgatatcc tcagctgtca atggaagtcc agctggaatt ccctcaataa
    20401 tagctgttag acgggggccg tgtgattctc ctgcagttaa atatctcata cactctcctt
    20461 attttaccaa gtagtctttc atctcttcca gagaaactgg gtgaatggtc gctgaaccaa
    20521 gctctggcac caagaccaat ttcaaggtgt taccacgcgc tttcttgtca tgagtaagag
    20581 cctgataaag cttgtcaact ttccaatttt catagtcaac aggcaagccg aatttctgac
    20641 acatctctgt gatagattgg gtaatgccag ctggcatgag gcctttttcc tcagcaacct
    20701 tggaaatctg taccattccc atggcaacag cctctccatg catgaccttg ccataaccgg
    20761 cagtcgcttc gatggcatgg ccaatagtgt ggccaaaatt gaggtaaaga cgaataccat
    20821 tgtccaactc atcttcaacc accatcttgc gcttcacctg acaagaatgt tcaatcaagg
    20881 tctctgcatg ttccaaaata ctctcaacag aaccattcag tcccgtcaag agagcccaca
    20941 gttctggatc ctcaatcaag ccatacttga taacttcacc catcccttca atcaactctc
    21001 tttttccgag ggtttcaaga acaagtggat caatcagaac cccatctggt tgggcaaagg
    21061 tccccaccat atttttagca aatggtgtgt taacgcctgt ctttccaccg atagaagaat
    21121 caacctgagc tgtcaaacta gtcggaatct gaacaaagtg aataccccgc atataggtag
    21181 aggctacaaa gccagccagg tccccaacaa cgccaccacc aagagcaacg attccatcgc
    21241 tacgagtcag accttgcttg actagaaatt catagacttt ctgaacagta gttaaattct
    21301 ttctttcttc accttctaag aaatcaaaaa cagctacctg aaaaccagca tcttctaggc
    21361 tgagcttgac cttctctgca tagagagagg ctacatggtt atctgtcaca atgactacct
    21421 tttgcggttg ccagagttct cgcaaccact gaccagcctg ggccatacaa cctttttcaa
    21481 tctgaatatc ataaggatga tgaggaatat cgattctgat tttcatagga gagtctccct
    21541 ttctttattg atatttttct gttaaagact gccaaatctc ttctgtcggc attttcttgc
    21601 ctgtccacag ttgaaaagct tctgcagctt gatagagcaa cattcccaga ccattgactg
    21661 ctggattgcc ctgacttcta gcccatttca aaaacggtgt ttcaaagggt tggtatatga
    21721 tatctgcaac taagagagtt tctggtaaga ctatgttttc aggaacagga gaggattggc
    21781 catccatgcc cacactggtg gcattaacta gcaaatccga ctcggcaatc cttgcttgca
    21841 gttcagaaac atattctaaa gcacacaaat ccactttaaa gcctgtctgc tcctgtaact
    21901 tgtctaggta aggtcttgtt ttttccatag aaacggaacg aacaaagacc gaaatctgac
    21961 tgacgccatc caaaatagcc tgtgccaaga ttgatttagc cgcaccacct gcacccagca
    22021 gggtcatctt tttacctgaa attgtaaaag aaggcaagca cttaaaaaat cccttgccat
    22081 ctgtattata tccaattaaa ttgccattct cattgacaac cgtattaacc gcaccaatca
    22141 agcgcgcttc atcgctcagc tcatccaaat aaggaatcac ctgctcctta tagggcatgg
    22201 acagattgat gccaaacatc tggtagcgac gaatattggc cactgtttct accaagtcac
    22261 tcgcttcaat ctcccaagcc acataagcac cgttggtagc tgtcgcctca aaggctctat
    22321 tgtggatgaa gggagaaata gaatgcttaa taggattggc aacaactgca gctaaacgtg
    22381 tatagccatc aagcttcatc caaaatctcc ctgatttttt tcatgctagc tagagaaatc
    22441 tgcccagggg cactaacctc atccagactg gcaaaagacc aactcgaacc agtcacatcc
    22501 gcagtgatac gagagacctt gcccacctta cccatagaaa tggtcacata ttcctgttca
    22561 ggattgagcg ttttaaagcc tcgtgtatag ttcatcaagt ctaagacatc ctgctccgtg
    22621 tgagccatca ccgcaacctt aacaagtttt ggatttagga tcgtcaactc tgacaagatt
    22681 tccatcatgt tctcaggtgt ttcttggaaa ttatggtaac tcaaaacaag atttgggaag
    22741 tccagcattt cctcaaaaac atccttgtag ctatagtact caaaatcaat atagtctggt
    22801 tgatagagtt gcgcaacttc cttgattaga tggatatact cttctggaga aaggtcgatt
    22861 tctccacctt cggagcgagt tcgtagcgtg aaaaccaact cacggcctgc gaatttttca
    22921 aaaatggctg gagctacctg caaaatcgct tctttaggca gatagtcggc acgccattca
    22981 atgatgtcgg catccaggta cctcgtggca tccagagcct gagcctcctc taaacttctt
    23041 ggcattactg aaacgattaa tttcatttac taaccttcat actaatcacc ttgaggtaat
    23101 tactactttc atctttttta ttataggcaa aatctgctgg aagaccatat ttgtttaaaa
    23161 tctggtaact tcttcctgca aagcctttat caatttgttc tgtaaatttc tgacgggaaa
    23221 cattggcagc attggtactg gcaatgataa tccctcccgg atttaaaatc tcaagactct
    23281 gggaaatcaa cttgtgataa tccttggcca cagagaaagt ttgtttttta ttccgagcaa
    23341 agctaggcgg atctaggaca atcacatcgt aggtcaagtt tttgcgtttg gcatatttga
    23401 aatactcaaa gacatccatg actataaaac gacgctcgtc tgtgctgagc ccatttgcct
    23461 gaaaatgcgc ttgagacaat tctcgtgaac gtttggctag atcaacagaa gttgtctggc
    23521 tagctcctcc catggccgca gctactgaaa aagccgctgt gtaggaaaac atattgagca
    23581 aggatttacc catagccaag ccgtcaacta aactaccgcg aacctcatgc tggtctagga
    23641 aaattcctgt catcaagcca tcattcataa agacttgata caggacacca ttttctaaaa
    23701 cagtgaaaaa gtcaggtgct tcttgaccat aaacatgggc agattcatag tccaaaccct
    23761 taaagcggat tttctcataa gcccctaaaa cctcagggaa aacctgtcta aaggcttctg
    23821 atatagtctg acgaatctga taaacataag agttatacca agaaaagacg gcgtagtcgc
    23881 cataaaggtc cactgtcaga cccccaaagc catctccctc ttgattaaag agacgaaagg
    23941 cagttgtcaa atcatcttga tagtaggcgt ttctcttttc tttggctttt ctaaacaacg
    24001 tttcaaagaa agcttgattg aaggccacct tgtctttgct gacaaaccag cccaagccct
    24061 tgttttgctg agaaaggtag gcagtcccaa gaaagtttcc ttcctgaccc tgcacctcta
    24121 cttcctgatc cttaagattg acattctcaa gatcactggc ttctagtaaa actagcccct
    24181 tagcaagctt cttttcaacc cttttgctga ctcttattct attcataact accattatat
    24241 caaactttta gacaattctc aaaaaagaaa ctacccttgc ttttttactc ttcttttaaa
    24301 aaatggtata ctagacttcc tgcaaaacta ggaagtaaat gtgtaagaat cacaataaaa
    24361 aatgctcttc cgtcttggag gagcatttct ttttatcaac gaaaatcaaa taacaaacta
    24421 tgaaactagc ctcaggttaa ctgtgagatt ataggtagag aggttgaatc agcaatatgt
    24481 gtctgtcaaa tttagtgaca aaggtagtag aagaaagatg aagaaataaa tctgcttcag
    24541 tagatatctg gaaaatttga ttttatagaa aagccttttg ttacaaactc aatatactat
    24601 caataaataa tattatagaa gcaacaataa ttaaaatttc acctatttgc atcattctat
    24661 ttcgaactct aaatatatgt tctatcaaaa atacttggat cacacacatt ataggaatta
    24721 acgtttttga aattgaaaaa tatcccaata aataaactat aaacaacaat aatagaacta
    24781 tattatattt cttattcaaa acattcctcc ctatattttt gattaccaat cttaatcatt
    24841 tacaactaca ttctaacaaa ctataaaagc gtttgtcgaa ttgaatttat caagcaagca
    24901 accaactatc tcatcttttt tctatttctg ccaatatgcg tgacaggtaa taatgatagc
    24961 caaaaatagc aagagcaagc aagacgataa gagctcctac tcccaagttg atggcaagga
    25021 taggggagag agactgaacc aagaatatgc tcccaattac aagggccatc aggattgcac
    25081 tataaataaa caataaaact atggcgacta tgccatttga acgattcacc aggtccgtaa
    25141 tgctactcca attggttgac agatttttaa cgtccttaaa gtaatggtgg caagaaagga
    25201 tgacactggc aatgatccag actacaagaa ggtaaatcat cgaaaggatg gacaagccta
    25261 gatatagaga aagaccgagc aaagtcagaa ctggtaaaaa ggactgaaca gcaaagataa
    25321 tccaaaattt cactttcaca taacgagcaa agtcaaaggg taaactcttc agaaaatcaa
    25381 cattttccct ctccaaggac aaggcaattg aatgcaggct ggtgatattg ttattgacaa
    25441 ctgctataaa cactcctaca aaaaacaagg gtaaccagta tggaggatga atgtctggaa
    25501 ctatctgaga atctcggatt ttggaaatca gactgatcat catgagataa ggaaggaaag
    25561 cacttgtaaa aagcactgta atcacaccag tcccctatcc caagagggtg aggtggtagc
    25621 gtaaaaccat acggaaaaag ccctttttag tagttgaaat cctctccttg ctgcgacgtt
    25681 cttttttgac cttctcctca ctgttaagca ggatcacgtc ataaaaacga ggaaggacct
    25741 tctttttggt cagataaagc aggaagagag ttagtcctat ccaagcgagc agacccacta
    25801 aggcttctgt cgaaaaaggc tccactgcta ttttgtaaaa gatatgaaga ggataaagga
    25861 gaaatggaat gtctctaact ttgtcaacaa tacttccaaa agtcgactga agaaagaaga
    25921 taaatattaa aggtatgaga actcctatcc caatcatcac attcgaaaaa atagactgat
    25981 actttctgaa gaccctagtc tgagccaaga aatgtactgc cactaccgtc actaaagtaa
    26041 cagagacaaa taataaggtc aaggacagta gcatcaaagg caaacccagc caaagagaag
    26101 gagctagact aatatagagg gctagaaaat aagctaggat tggtacaatt ccagttagag
    26161 ctggcaagag gacagacaga cctttggcca ttataatctc tgattcttta aaggcatagg
    26221 gcctatagga ggccaggtcc ttactctcat aaaagacatt gtaaaaggca gtgaaagaag
    26281 ttgaaaaggc cagcaccagt aaaatagcaa ccatcgagct aaaataaata ggtatttcct
    26341 caaaaggaaa atgaatggct atattactaa aacagatgag catcaagaga ctggaaaaaa
    26401 tgtaagaact taggactcta gcagaaacat ttactttttt cccaggattc ttagcctgct
    26461 tctttcgtag attagccaga ttagcttctt gagatgaata gaggatattg atatcaacta
    26521 attttttaat gaccttgaga cgcatccgca acctcctctt ttctaccagc aagactaagg
    26581 tagatacttt ccaaaaactg gtctgggtgg tctttcctca agttctctac actaccacaa
    26641 tagatcaaat gccccttttt caaaatagca atccgatcac agacttgctc ggctacctca
    26701 aggacatgag ttgaaaacaa gaccgtcttg cctttttgtg catgttcctt catcatttgc
    26761 ttcaaatcaa aggcggcctg gggatccaaa ccagtcaagg gttcgtccaa aacccaaata
    26821 tcaggagcag acaagagtgc cccaatgaca aagactttct gacgcattcc gtgagaaaga
    26881 gtttcaataa cctgatagcg gttttcagca aaatcaaaaa cgttcaatag cctagctaga
    26941 ctagtctcca agtcagagct agtcagatca taggatgaag cgatcaattc ccaaaattca
    27001 ttggccgtta agcgtaaaaa taagtcaggc gagtctgcta cgtagccaat ctttcgtttg
    27061 atcgccaagc gattttccga taactcctga ccgtctacca aaatacgacc actgctgggt
    27121 gaaatgatac tgactaggga ttttatggtg gtcgattttc cagccccatt atgaccaatc
    27181 aagcccataa tctctccatt ttcaatctgc aaattgagat tactcaaggc ctctttatca
    27241 ccatacaact tactaacatt ttgaaattca atcatgggac aactcctatc tttatctgta
    27301 ttatacatcg ctatactagc actttgatac aaaaaaatca atactttatt ttaagtagtt
    27361 aaaatagttt ccttctatct tacgcaaacg tttgcgccga gagatacttt atgatagaat
    27421 aaagaacaag attgacaagt aagaggaaac atcatgcaaa atcaaacact catgcaatac
    27481 tttgaatggt atctgcccca cgacggtcaa cactggacgc gtctggctga aaatgctcca
    27541 cacctagctc atctggggat cagtcacgtc tggatgccac cagccttcaa ggcaaccaac
    27601 gaaaaagatg tcggctatgg ggtctatgac ttatttgact taggagagtt caaccaaaaa
    27661 gggactgtcc gcaccaagta tggtttcaaa gaagactatc ttcaagccat tcaagccctt
    27721 aaagaacagg gaattcaacc tatggccgat gtagttctca accacaaggc tgctgccgat
    27781 aacagggaag cctttcaggt tatcgaagtt gatcctgtag accgtacagt tgaacttgga
    27841 gaacccttca ccatcaatgg ctggactagt tttaccttcg atggtcgcca agatacctat
    27901 aatggcttcc actggcattg gtaccacttc accggtacag actacgatgc caaacgcagt
    27961 aaatctggga tttatctgat ccaaggggac aacaagggct gggctaacga ggaattggtc
    28021 gataacgaaa acggaaacta cgactacctc atgtatgccg acctagactt taaacatcct
    28081 gaagtcatcc aaaacatcta tgactgggct gattggttca tggaaacgac tggtgtagct
    28141 ggtttccgtt tggatgccgt taagcatatt gactctttct ttatgcgcaa cttcatccgc
    28201 gatatgaagg aaaaatacgg tgacgatttc tatgtttttg gtgaattttg gaacccagac
    28261 aaggaagcca atctggacta tctcgaaaaa acggaagaac actttgacct tgtcgatgtt
    28321 cgtctccacc agaatctctt tgaagccagt caagctggcg caaactatga ccttcgtggc
    28381 attttcacag atagcctggt tgaactcaag cctgacaagg ctgtgacttt tgtcgacaac
    28441 cacgataccc aacgaggaca agcccttgag tctaccgttg aagaatggtt caagccagca
    28501 gcctatgccc tcattttgtt acgccaagac ggccttccat gtgtctttta cggagactac
    28561 tatgggattt caggccagta tgctcaagaa gatttcaaag aaatccttga ccgcctccta
    28621 gccatccgaa aagacttggc ctatggagaa caaaatgact actttgacca tgctaactgt
    28681 atcggttggg tacgttcagg tgctgaaaat caatccccaa tcgcagtcct tatctcaaat
    28741 gaccaagaaa acagcaagtc aatgtttgtc ggtcaagaat ggactaatca aacctttgta
    28801 gatttacttg gtaaccacca aggtcaagtt acaattgatg aggaaggtta tggacaattc
    28861 cctgtctcag ctagatccgt aagtgtctgg gcagtcaata ccatctaata gctcataata
    28921 accaagctag gtccaagcgg atttggcttt tttgtattca caaaaagacc tacccaaatg
    28981 gatagatctt tacttgatta caatttacct gctactgcat ccaacaattc ttggatctta
    29041 gcttggttgc ttcctcctgc catggccatg tctggtttac caccaccacg tccatcgatg
    29101 attggtgcta attctttgac aaggtttcct gcatgaaggt cttttgtctt gcttgctaca
    29161 aggacattga ctttgtcacc gatagcggca actaggacaa gaagatcaga gtagtctttt
    29221 tgtttccagt tatctgcaaa agtacgaagg gcaccggcat cggatacaga cacttgacta
    29281 gcaatgtaac gatgaccgtt gacttcctta gcatctttga agatatcgcc tgcggctgca
    29341 gctgcggctt tttctttcaa ctcagcattt tctttttgaa gttgacgaag ttgttcttga
    29401 agtccttcta ccttgtgagg tacttccttg acttgaggtg ctttcaaggt tgctgcgaca
    29461 gctttaagag catcctcttg ttcacgatag gcttcaaagg cttccttacc agtcactgcc
    29521 aagatacggc gagttcctga accgattcct tcttctttga caattttgaa gagaccaatc
    29581 tcagaagtgt tgccaacatg agtaccacca caaagttcaa tagagtagtc accgatagtc
    29641 acgacacgaa cttccttgcc gtatttctca ccaaagaggg ccatagctcc catttcttta
    29701 gcagtgtcaa tatccgtttc aactgtcttc acttcaagtg cttcccaaat tttctcgtta
    29761 acttgctgtt caatcgcacg aagttcctca gcagttactg cttggaagtg ggtaaagtca
    29821 aagcgaagga attcaacttc gttaagagat cctgcctgtg ttgcgtggtt tccaaggata
    29881 ttgtgaaggg cagcgtgaag caaatgagtc gcagtgtggt ttttcatgac acggtgacgg
    29941 cgattgctat caattgccaa ggtatattct tggttcaagg caagcggtgc aaggacttca
    30001 actgtatgaa gggcttgacc atttggggct ttctgaacat tggtcacagt agccacaatc
    30061 ttacctgact catccaagat ttgtccgtag tcagctacct gtccacccat ttcagcataa
    30121 aatgacgttt ccgcaaagat aagagaggca gttccttctg aaacagctcc tacttctgca
    30181 ttgtcagcaa cgatagctac caatttagaa gacaattggc tagtattgta gttgaagaca
    30241 ctttctacag tgatgttttg aagagtttca ttttgcatac ccattgagcc acccttgaca
    30301 gctgacgcac gcgcgcgttc ttgttgttct ttcatggctg cttcaaaacc ttcacggtct
    30361 acagtcatac cagcctcttc agcaatttct tcagtcaatt caactgggaa cccataagta
    30421 tcatagagtt tgaagacatc tgaaccagcg ataacagatt gacctttttc tttcaagtct
    30481 gctacaatgc cttgggcaaa gtgttgacct gagtgaaggg tacgggcaaa tgattcttct
    30541 tcgctcttaa cgattttctc aataaagtca cgtttctcaa gcacttctgg gtagtagctt
    30601 tccatgattt ttccaacagt tggaaccaat ttgtaaagga aaggctcgtt gattcccaat
    30661 ttttgaccat gcatagaagc acgacggaga agacgacgaa gaacataacc acgaccttca
    30721 tttcctggaa gggcaccatc accgatggca aatgaaagtg aacggatgtg gtcagcgatg
    30781 accttgaagc tcatgttgtc gccatcttgg tcataaacct taccagacaa tttctcgact
    30841 tcacggataa tcggcatgaa gaggtccgtt tcaaagttgg tcttagcccc ttggataacg
    30901 gccaccaaac gctccaaacc agcgcccgta tcaatgttct tatgtggcaa ttccttgtat
    30961 tcgctacgag gaacagcagg gtctgcgtta aattgtgaca aaacgatgtt ccagatttca
    31021 atataacggt cgttttcaat atcttctgca agcaggcgaa gaccgatatt ttctgggtca
    31081 aaggcttccc cacggtcaaa gaagatttct gtatctggtc cagaaggtcc cgcaccgatt
    31141 tcccagaagt tgtcctcaat tggaatcaag tgacttggat ccactcccac ttcaatccag
    31201 cggttgtaag aatctttatc gtctggatag taggtcatgt aaagtttttc agcagggaaa
    31261 tcaaaccatt cagggcttat caaaagctca taagcccaag tgatagcttc gtcacggaag
    31321 taatccccga tagagaagtt ccccaacatt tcaaacatgg tatggtgacg cgcagtcttc
    31381 cctacgtttt cgatgtcgtt ggtacggata gccttttggg cattggtaat acgtggattt
    31441 tcagggataa tggtcccgtc aaagtatttc ttaagggttg ctaccccaga gttgatccac
    31501 aaaagagttg ggtcatttac aggaaccaaa cttactgatg gttctactga gtgacctttg
    31561 gtcgcccaga aatcaagcca catttggcgt acttgtgcac tagatagttg tttcatattg
    31621 tctccttatt tacttgttta atgtgattgg ctttccagca tttccacata gtcaatcgcg
    31681 acacagaggg aaatgactag gtctgcataa gcgtcttcaa gaaccgttac ggtataggta
    31741 gaggtcagat ggaagagttc cttcttaatt tccgcaatca actgatcgcg atcatccagc
    31801 aatttgaaat tcaaatccca gatattgccc tcgatacgaa gacctagatt atcaaactca
    31861 tacttatctc gccaaaaggt caacttctta cgaatgacaa aactcgagcc atccagaagc
    31921 tgaatctcaa aacgaggaag caaggtcaag atttctttac tgatctgact gacttgttca
    31981 ccagccgcat catagatggt aaaagttttg ggaatcttaa aaaatgatcc ctccacctga
    32041 taggcaattt ctcccctgtc atccttgata gcgaagcgtt cgcctccaag acgaaacttt
    32101 tgtttgacaa gaaatgtttt catcaacacc tccaaaaatc aaaagacaag ctcatatcac
    32161 gaagggcgaa aaaccgcggt accaccttca ttcaatgaac ttgtcattct cttgttctta
    32221 tgcaattgta tgattgagta gcatgacttc ctagcttaga tggctcgcag caccgccatt
    32281 tctctggact aagacaagtg aaaatcaatt ctcaactttc ttattatatc gtttttttaa
    32341 tcttgcgtca actggaaatg atctccgttg aattagacca attccctaca tctctgatta
    32401 cttttccagg atatattttt tcttactgcc gtttttcttt ttatcccaaa ttttcatatt
    32461 actaaacaca gctactagaa tatttccaaa tataaaggtg cctatcaccc aatatatgga
    32521 ctcagttgtt aggtattgtc gatccaagcc atcctttaaa tggaatagta tagcagtttg
    32581 gttaacaatc ataaaggttg gccagaaact tttgttgaaa aaagtagaca ttttcattat
    32641 ttgttgccgc tttctgtaag gttaatactc aataaaaatc aaaaagcaaa ctaggaagct
    32701 agcctcaagc tgtacttgag tacggcaagg caacgctgac gtggtttgaa gagtataggc
    32761 ttagtatact actaggcaag caaataagca aataaacaac tagaatagaa aaagataggg
    32821 ctctaaaaac tgacttctat tccttaaaaa cgaaccagct tgactgattc gtcttcttac
    32881 gtttatctcc tacttccgat acattttaaa ctgtaggaag aggtcgctat atttccctgt
    32941 ccatttatgg tcaaatttct cataaatttc taggtgtttc atggtttcaa catcgggata
    33001 gaaggcctta tcttcctttg tttcctctgg gagcaattcc ttcgctggta tgtttggtgt
    33061 tgaatagccg acatactccg cattttggag agcattttca ggtttcaaca taaagttgat
    33121 aaaggcatag gctgagtctt ggtttttaac tgttttggga atgaccatat tgtcaaacca
    33181 aagattgctg gcctctgtcg gtaccacata acgtagattt tcattttttt ctaacatttg
    33241 gctggcttca ccagagaagg tcacgccgat tgcaacgtta ttctgaatca tatagccctt
    33301 catctcgtcc gcaacgatag ccttgatatt tggagtcagt ttgtagagct tatccactgt
    33361 ctcttccaac tgctgcagat ccttggagtt gaggctgtag ccgagggaat tgagtcctag
    33421 tcccagcacc tcacgcgccc catcaaagag catgatagaa ttcttatact ccggcttcca
    33481 aaggtcatcc caatgctcag gcgcttcatc taccatggtt tcgttgtaga caattcctaa
    33541 ggttccccag aagtaaggga tggagaattt attacctggg tcaaaggact ggttgagaaa
    33601 ctctggtccg atattttcga ttccttcaat ttttgaataa tcaagcggaa ccaagaggtc
    33661 ttcgtccttc atcttgttaa tcatgtattc acttggaatg gcaatatcgt aggtcgttcc
    33721 accctgcttt atcttagtgt acatggcttc gttggagtca aaagtctcgt actgaacttg
    33781 aattcctgtt tcttctgtaa actgagtcaa gagttcagga tcgatatagt ctccccagtt
    33841 atagataacc aatttttgac tatctcgact attgatttta ctatctaaat gagtcgcaat
    33901 tccccacaag acaaggataa tcgctgcaat tcctgctaaa aatgaataga tttttttcat
    33961 gcttgctcct ccttctcacg agagataaag taataaccta caactaggat aatactaaag
    34021 agaaagacta gagcagacag ggcattgatt tctaaggaaa tccccttgcg agcacgagag
    34081 taaatctcga ctgatagggt tgaaaagcca tttcctgtta caaagaaggt cacggcaaag
    34141 tcatctaacg aataggtgaa ggccatgaaa taaccagtaa tgatagacgg agtcaggtaa
    34201 ggaagcatga tttccttgaa catctgaaat tgactagctc ccaagtcata ggccgcatga
    34261 atcatgtcgc cattcatttc cttgagtcga ggcaagacca tcaagaccac gataggaatg
    34321 gagaaggcca cgtgactaga tagaacggtc aaaaagccaa gtgaaaactt gagttgggta
    34381 aagagaatca agaagctagc accaatcata acgtcaggcg caaccatgag gatattattg
    34441 agtgatagaa aggcttcttg gtatttctta cgagactggt agatgtaaat ggcaccaaaa
    34501 gtcccgataa tggtcgctat caaggctgat aggaaggcca agaaaaatgt ctgagccaaa
    34561 atcagcatga gtctcccatc tccaaacatg gtttcaaagt gagtccagct aaaacctgta
    34621 aagctattca tatcatcacc agcattaaag gcatagccaa tcaagtaaaa gataggcagg
    34681 tagaggacca gaaagaccag tcccagataa aggttggcaa attttttcat cgttctctcc
    34741 tttccttagt cacccacatg gtgatgaaca tggtcaggat gagaatcaca ccgatggttg
    34801 aacccatacc atagttgtca ttggttagaa aattctgctc aatagccgtc cccaaggtga
    34861 taacgcggtt cccaccaatc aaacgggtca gcatgaagag actcaaactt gggataaaga
    34921 ccgactgaac cccacttcgc acaccgttca tagatagagg gaagatgaca tgacggaagg
    34981 tctcccactt agttgcacca aggtcataac tagcattgat gagattatta tccatgtcgt
    35041 ctaagacatt gaaaatcggc aaaatcataa aggggagctc gatgtagctt gcgacaaaga
    35101 taaaggaaaa atcggtaaaa agcaactgtt gtgaaccaat tccgataaat tccaagaatt
    35161 ggttaataga gccattttga ccaaaaatcc cgataaaagc ataggcttta aggagcaaat
    35221 tgatccaggt aggaaggata atcagcatga gccagagttg acggtgcttg agacgggtca
    35281 aaaagagggc tgttggatag ctgataagca aggtcacaaa ggtcacaatc cctgcataaa
    35341 gcactgagtt gaaactcatt ttgaggtagg tcaagttttg tgacgcaaag taagatttat
    35401 aattttctaa actaaactgg ccttcaatgt tgaaaaagga ttgtccgaaa atcaagacca
    35461 agggtgccaa tacaaagagg gcaatccaaa gcatgtaggg cactacaaag agtttagagc
    35521 ttgttttctt catctctttc ctcctcgatc gcattgatca gacctgcttc ttgctcttcg
    35581 atttccacgt attcttcgat acgagcatcg aactcttctt cggtttcatt gagacgcatg
    35641 atgtggatgt cttctgattc aaagtccaga ccgatttcct cacccacgat ggccttacgg
    35701 gttgagtgga tcatccattc atttccaagt tcgtcatagg cgataatttc ataatgaact
    35761 ccacggaaga gctgggtgtc gaccttaact tggagtttgc cttcttcagg aagggtaatg
    35821 cgcaagtcct ctggacgaat aacgacctca acaggttcat ttggcttcat cccaccatca
    35881 accgcttcaa agcgtttgcc gttaaattcg accaagtagt cctcaatcat ggtacctggc
    35941 aagatgtttg actccccgat aaaggtggca acaaagtggt tgattggctc atcgtagatg
    36001 tccacagggg ttccagactg gacaatctcg ccatcattca taacgaaaat ccagtcactc
    36061 atggcaagag cttcttcctg atcgtgagtg acaaagacaa aggtaatgcc caatcgttgt
    36121 tgtaattcac gcaattcgta ctgcatgtct gttctcaatt tcaagtccag cgctgataaa
    36181 ggctcgtcca acaagaccac acggggttgg ttgatgatag cacgggcgat ggccacacgc
    36241 tgacgttgtc ctccagaaag tttgcggatg gaacgttttt cataaccttc caactgaacc
    36301 atcttgagaa cttccgctac acgctgctcg atttctttct tatcaatttt acgcaagcga
    36361 agtggaaagg caacattttc aaacacattc atatgtggga acaaggcata ggattggaag
    36421 acggtatgta cgtcgcgctt gttggttgga atatcattga tacgaacacc gtctagcatg
    36481 atatctcctg tcgtcgcatc cagtaaacct gcaataatgt ttaggatagt tgatttcccc
    36541 gaaccagatg cacctagaag ggtgtagaat ttcccttctt ccaactcaaa gttgatgtct
    36601 ttgagaacct tggtgttgct gtcttcaaaa actttagaga cgtttttgaa ttcgataatt
    36661 ggttttttca attggcataa attccttctt tttcatagat taaccgatcg gggctctgtc
    36721 aggtccccac tacctcttgc agggagtaaa accacctgca tacatcttcg ctaccgatag
    36781 gctttcaccc aagatccgga cttctctttc aagcgtaata cctgagtgtt ccttgacttt
    36841 ttcgataacc gattggatca agtcctcgta gtctttggcc gttccatctg cgacattgat
    36901 cataaatcct gcatgctttt ctgacacttc tacgccaccg atacgatagc ctttcaagcc
    36961 agcttctgaa attaactgac ctgcaaaatg cccgactgga cgcttaaaga ccgagccaca
    37021 agatgggtat tccaaaggtt gcttgagttc acgtaggtgc gtcaagcggt ccatttcctg
    37081 cttgataacc tgatgggttc ctggagctag ggcaaattta actgacaaga caactgcacc
    37141 agactcctga atagctgaat ggcggtaacc aaaagccaag tctttagcag acagggtttc
    37201 gatttctcca tccttggtca agaccttaca agactgcaag atgtgagcaa tctcgccacc
    37261 ataggcaccc gcattcataa agacagcacc gccaacgctt cctggaatac cacaagcaaa
    37321 ctcaaagcca gttaaactat gacggagggc aatgcgagtt gtttcaatca agttagcccc
    37381 agcttctgct tcaatggtat agccatcaac agaaacgtta ttgagcttgt cacacaagat
    37441 gacaaatcca cgaatcccac catcacgaac gatgatattg cttgcattgc caagaaccat
    37501 ccaagggata ttttcttggt tggcaaattt cacaaggcga gccaactcaa aacgatttcg
    37561 tggaaagacc aaataatcag cctctccacc tacttttgta taactatagc tatgcaaggg
    37621 ttcctgaaaa cggatatcaa ttccttctaa gatttcaagc attttttctc ttacagacat
    37681 gtcactcttc cttttccaaa attcattcca ttataccatt tttagagaca tttgacgacc
    37741 ataaaaatac cttgtttgga ttttgcataa gaaaaagagg ttcccccctt tttatgattt
    37801 tttacaaaag atttccttgg ttccataggc gaccagaacg agctccagtg ctagaatcac
    37861 ttcaaccaag actggatttg tcaaccagcc tacttggaaa agagatggtg ccagatcaaa
    37921 gaaggcatgc aagccatagg ctgttaggag ataaatccat ttcttctggc gaacagcttg
    37981 gtaaacccaa actgtcaaaa gtaattggaa accaagcgcc aagattcgct caaaaccaag
    38041 caaataaatc tgccagaccg aaagtgactg aatggttttt aacatatttt cagacagtaa
    38101 ttgcataacc tgtggattct gagtttgaac tgccgaaaga acaatgtaaa gattgagtaa
    38161 actagtaagg cctagaaaaa tcaactccaa gccaccatgc cccagtccat aggccaaggc
    38221 gtctgccttt tccaaacttc tctttttctc aaaccatttg aagaaaacaa gacgagccgt
    38281 ttcctcaaaa aatgctgcca tggctaggcc atagataata tagataagcg gatgatcttg
    38341 caagagggca atactaccgt ctttttgagg gtgcaaaatc aagatatgca ccagtttttc
    38401 caaaatctgc gaagagacaa agaaagcgac tgcacccaag cccaagacag ctagattaat
    38461 ctggtatttc tttttggcaa accaaatgct tcctatcaga aaagctagca acagcaacat
    38521 ggtaataatg atatgaatgg tcattttctt ctcctattct gccttttcaa tatctttttt
    38581 catctcgtca acattgaact tagcaaacaa gtattgacgg tcttggactg ggaaacgttg
    38641 gtccaactgg tcaactgctc ccacctcgat aatgccttcc ttgatgacaa aatccatgac
    38701 atgtccaccg aaggtcaaat catctgatat gaagtgcaga tggtagcctg ccacactgac
    38761 cccatggaaa atctcaggcg tccagaaacc aacaatagtc cccgcaacat tgtcacgact
    38821 atattccggt tgatgggttg cgacatcagc aaacttggta tcgggtgttg acttaggaat
    38881 catacgcaca tgcatatgta aaaattcccc ccgaatcttg atagagcgga aaagattttc
    38941 cccatcataa taagactcaa ttcgttcttt caattccttg tctgtcatct caaagcgctg
    39001 gcggaaaatg acctctgcct gatgcggtac cactgcagcg taaggaataa gggcatctgg
    39061 tgacacttcc acaatttctg gctggtctcc tgaccctttg gcctgataag ccttgccatc
    39121 caagacaatc aattccccat caatggaatc caaggttccc aaaccaaggt caccatgttc
    39181 tagcaattct cctactgtca tagtaccacc ataaaggcca gccatcaagg ctccaagagt
    39241 attgtattga aataatttca ctggttcctg cacgttctta tccattcact ttcttgtctg
    39301 ttattctata aatcttactc ctaagtatac cacatttgcc cctagatgtg aacgagagaa
    39361 acgttctaga cattgccaag aaggaaaaaa aagggtacaa tgtaacaaaa tcaagggagg
    39421 tctggaatga agaaacaaag caagtacaaa gaggtcgttt cctatctgaa aaatggtatc
    39481 gagtctggac gatttccgac gggtagtcgc ctgccttcta tccgtcaact gagccttgac
    39541 tttcactgca gcaaggacac cattcaacga gccctgctgg aattacggca cgaacaatac
    39601 ctctatgcca agcctcagag tggctactat gtattagaac aagggcaaca tcaagaccta
    39661 gaaatcgagg ttaccgacga acatgccagt gcctatgacg atttccgact ctgtgtcaat
    39721 gaaaccttga ttggccgaga aaactacctc ttcaactact atgacaatca agaaggatta
    39781 gaagacctaa gacagtccat tcacaaactc ctctttgagc aagctctcta ctgcaaggct
    39841 aaccaactag tactgacttc tggaacccaa caagccttat ttatcctctc tcaaatatcc
    39901 tttcctagac aagccaagga aatcttggtg gaacagccaa cctaccatcg gatgaatcgc
    39961 ctcttgattg cacaggggct ggactatcaa acgattgaac gaggcattga tgggattgac
    40021 ttggaggagc tggaaggcca cttcaaaaca ggaaaaatta aatttttcta caccattccc
    40081 cgatttcact atcctctggg acattcctat tctgagcaag acaaacgatc tattcttaac
    40141 ttagctgcca agtatgatgt ctatatcgta gaggacgatt atctgggtga tttggactcc
    40201 aagaagggcc aaaccttcca ctatcttgat acagaggagc atgtcattta tatcaagtcc
    40261 ttctcgacca gcctttttcc tgcccttcgt attacagcac tcattcttcc aaatgctatc
    40321 aaagaagcat ttgtggccta caaaaatatc ctagactacg acagcaacct cattatgcaa
    40381 aaggccctgt cactctatat tgacagtcaa ttgtttgaaa aaaatcgttt ggctcgcttg
    40441 accaatcatg aatcttacca aaaacaaatc gaggaaagga taactaaaac accttgtccc
    40501 cttcctcatt atcccctaca cgatggttta ttgctagacc tgagacagta tcctaaaatc
    40561 gccagtctca aacacagtca actgggcttg gacttctttg aagaggccta tttgagcacc
    40621 tgtccttatc aatttgccaa ggtgtcccta gacaatctgg aaaatgtttt aaactattta
    40681 aaagcagaat tggaatgaca tccaactctg ctttttctta ttcttcaact tcagttagac
    40741 ttgctgcttt ttcaagatag ctttggtaag ttccttcatc cttaagtttt tggatgacct
    40801 tgtctaccac ttctttcaaa tcatcactat tttttctaag agcaacggca ttggcgtcgc
    40861 cgtccttcat cttcaagctg acagttgcga cagctaagcc agcgttttta gcagcataac
    40921 taagtgcaac aggctcatcc atatgaacag catctatttt tccagcctgc aattcattga
    40981 ctgcttcacc catattagtt agggaagtta attgagcttt tggcaattgt tccttgacca
    41041 ttgattctgg aacagtccct ttttgggctg caatattagc actttctagg ctagttaaat
    41101 ccttgtattt ttccacatca gccttacgaa ccaagaaact aatcttgttt tcatagtatg
    41161 ggattgaaaa atcaaagact tcttttctct cgtcagtagc actaattcct gcaactgcta
    41221 ggtcagcctt accagtttga agactggtca aaacattgtc aaaactcatg cttaagattt
    41281 ccaacttaac cccaagttca tcagcgatag cctgagccat gtcgatgtct gcaccgacta
    41341 cctggttctt tccatcaacc aatgattgaa attcaaaggg tgcatagtca ggactggttg
    41401 ccacaactaa tttccccttt tgcttaatgg cctcaacagc tgactgagaa ccattagaac
    41461 ctgactggca agctactaag aagaaacttg caagaaaatt acataaaaca aatatccatt
    41521 tttttgattt cataaataaa caacctttct gttaattttg tataattata acgatatcca
    41581 agttacttgt caagtgtttt ttaaattttt atctcaaaaa tattttttcg ttcaaaaaaa
    41641 ggagccatca gttgatttca agctcctttt tatacagaat taaactattt tatagttcga
    41701 caatcttacc tgtttcaaag tagacaaccc attcacagat atttttagca tagtcaccga
    41761 tacgctccaa gtaggaaata acttggaaat aatcacgacc cgtaacaatg gcttctggat
    41821 ttttcttaat ctcttcagtc gcaaggtcac ggatagtttc aaaatagtgg ttaatttgct
    41881 catccatgga ggccacccgg tatgcgtcgt caacagaacc attaagataa agatcaagtg
    41941 ctgcttccac aacgctttta acttcacgtc ccattttttt aatttcttcc tctacagctg
    42001 ggatgcgttc ttcccccttc atacggatgg ttgcctgggc aatggctaca gcgtgatccc
    42061 ccatacgctc cacatctgat acagccttaa ggacagtcaa gactgtacgc aaatcttgag
    42121 agactggttg ttggagtgcg atcatttcaa atgatttctt ttccagtttc acttcgtatt
    42181 catttacttc tgcatcatct tcgatgacct cttttgccag gtcacggtca tgcgtgacaa
    42241 aagcacgtac cgtacgattg atttgtgaga gcacttcttg tcccatagcg tagaactggt
    42301 tatgtaattt ctctaaatct tcttcaaatt gagatcgtaa catctttcat ctccttatcc
    42361 aaattttcct gtaatatagt cttccgtttc cttgtgttgg ggatcaagga acatctgctt
    42421 ggtatcatta aattcaatca aatctccatc taggaaaaat cctgtcttat cagagatacg
    42481 tgaagcttgc tgcatggaac gggttaccag aagcatggtg tacttgtctt ttagaccata
    42541 caaggtttcc tcaattttac cagctgaaat cggatccaaa gccgaagttg gctcatccaa
    42601 gaggatgatt ttaggactag ttgccaagac acgggccacg cagacacgct gctgttgacc
    42661 acctgacaat ccaatagctg aatcatatag acgatccttg acctcatccc agatagaggc
    42721 accttgcaag gctttttcta cggcttcatc cagaacctgc ttatccttaa ttccattgat
    42781 acgaagcccg tagacaacat tctcatagat agtcataggg aaaggattag gttgttggaa
    42841 aaccattccg atttccttac gtaattcaac cgtatctgta cgcggactgt agatgttgtg
    42901 accattgtac accacggatc cagttgtggt cacctctgga ttgagatctc ccatgcggtt
    42961 gagagacttg aggagggttg acttccctga tccagatgga ccaatcaagg ctgtaatttc
    43021 cttaggttgg aaagataggg aaacactatt caaagccttc tttttattat aatatacgga
    43081 caggtctgat acctgtaaaa tcgcatctgt catacggttt cctttctaac caaagtgacc
    43141 agatacatag tcattggtgg actgtagctt ggcattttgg aaaatagttg cagtcttgtc
    43201 atactcaatc aaatcaccca agtaaaagaa gcctgtatag tcacttgcac gagcagcctg
    43261 ctgcatatta tgcgttacaa tgatgatggt aaagtttttc ttgagctcaa acatggtctc
    43321 ttctagttgc atggtcgcaa tcggatccaa ggctgaggct ggctcatcca ttaagaggat
    43381 atctggctta acagagatgg cacgagcgat acagagacgt tgttgctgac cacctgataa
    43441 ggtcaaggct gacttgtgga gatcgtcttt aacctgatcc cagagggcag cctgacgaag
    43501 ggaggtttct acgatttcat ctaggacttg cttatcctta actccagcac gttcatgcgc
    43561 aaaggtaata ttacggtaaa ttgacttagc aaatggattg gggcgttgaa aaaccattcc
    43621 aatgtgttta cgcatttcat aaacgttgat ttctggacgg ttgacatcaa ttccacgata
    43681 gagaatctgc ccagttactt tagcaatatc aatagtatca ttcatgcgat tgagactgcg
    43741 taagtaggta gatttccccg atcccgacgg accaatcaaa gctgtaattt tatttctttc
    43801 aaattgcata tcaatcccct taatggattc atttttacca tagtaaacat ggacatcctt
    43861 agtagaaagg gctacttttt cttcaggaaa ggtaaggata tgcttctcat cccagttata
    43921 tgttgacatg gcttctcctt taggcagcgg ttaatttctt gtgtagatag cttccgaact
    43981 tacgagctcc aaagttaaaa atcaggataa agatcaggag cacagcggca gaacctgctg
    44041 atacaatggt tccatctgga atagtgcctt cactattgac tttccagata tggacagcca
    44101 aggtttctgc ttgacggaag atagagatgg ggctagtcac actgaggata ttccagttag
    44161 accagtcaag agctggcgcc gattgccctg ctgtatagat cagagctgca gcttcgccaa
    44221 agatacgacc agatgccaag acgacacccg ttacaatacc tggaagcgct tctggaataa
    44281 caacatgaac cactgtctcc cagcgagaaa tcccaagagc cagaccagcc tcacgttggg
    44341 tatggtgaac gtgtttcaaa ctatcctcta cattacgcgt catctgaggc aagttaaaga
    44401 ctgtcaaggc caaggcacct gaaatgattg aaaatccata ctcaaactgg actacaaaga
    44461 tcaagtaacc aaagagaccc accaccactg atggtaaaga ggacaagatt tcaatacaag
    44521 tccgcacaaa gttggtaaca ggaccttttt tagcatattc agccaagtaa atcccagctc
    44581 ccatagaaag aggtacagaa ataatcaagg taatgaccaa taggaaaaag gaattgtaaa
    44641 gctgaatgcc aatcccacca cctgcttgaa aagcagaaga ccttccagtc aagaaagacc
    44701 aagagatatg gggcaagccc cgaaccaaga tatagagaat caaggaagcc aagattgtca
    44761 caatgatgct agcaatcgta tagaggacag ctgttgcaag tttatctaat ttcttagcgc
    44821 gcataatttt tctttcctct ttctttcgta atcaatttaa tcacactgtt aaaaactaaa
    44881 ctcatcaaga gcagtaccaa ggccagtgac cagagaacat tattatttac agttcccatg
    44941 acagtgttcc caattcccat agttaatata gaagttaaag ttgcagctgg tgtggtcaag
    45001 gaagttggga taacagctga gtttccgaca accatctgga tagctaaagc ctcaccaaag
    45061 gcacgcgcca tcccaaagac cactgcagtg aaaataccag aacgggccgc cttcaagatc
    45121 acacgccaga tagtctgcca gcgagtggct cccatagcga aacttgcctc acgataataa
    45181 cgaggaaccg cacgcaagct atccgttgtc ataaaggtta cggtcggcaa aatcatgaca
    45241 aagaggacgg aaatccctga caaaatccca aaaccagtcc caccaaagac actgcgaaca
    45301 aagggaacga cgacttgcaa gccaataaat ccgtacacta ctgaaggaat cccaaccagg
    45361 agttcaatag ctggttgcaa aatcttcgcc ccttttggtg atacttcggt cataaaaact
    45421 gctgcaccaa tagcaaaggg tgttgcgata agggctgaga gaatggtaac gataaaggaa
    45481 cccaaaatca taggaagggc accaaattct ttactagaag gattccaagt tcctcccaaa
    45541 agaaagtcaa agatattcac accattgaca aagaaggtcg acaagccttt ttgcgctacg
    45601 aaaaccaaaa tcatggccac aaggatgact atcaaagaaa gacaggcaaa ggtcaaacct
    45661 tttcctaatt tctccagacg agaattcttt gatggaagca acattttctt agctaattct
    45721 tcttgattca ttattgtctc ccttccaaca ctgtcacagt tccggcagca tctttttcaa
    45781 ccttcatttc cttaatcgga atatacttca atcctttgac aatcccttct tgggtctcat
    45841 ccgagagaac aaaattgaga aattctgcag ctaactcatt gggctgcccc aatgtataca
    45901 tatgctcata agaccacaag ggccaattat tgctacttat attttctgga cttaagtcat
    45961 agccattcaa cttcatgctt ttgaccgaat catctatata ggtaagagat aaataagaga
    46021 tagctcctgg actttttgat acgattgatt ttaccgctcc atttgaatcc tgctcctgac
    46081 tttgcatggc agactgacct tccataatga cagtatcaaa ggtagcacga gagccagagc
    46141 cggctgcccg attgataaca gagatgggta agtccttacc accaacctct ttccaattgg
    46201 ttacctcacc tatgaagatt tgacgaagtt gctctgtcgt taggttatca acatcaacct
    46261 ccttattgac aatcagagcc aagccagcta ccgcgacctt gtggtcaaca agagcagaag
    46321 catcaattcc gtctttttcc tcagcaaata catctgagtt tcctatatca actgccccag
    46381 actgaacctg ggacaagcct gtaccagaac ctcccccttg gacattgacc gtttttccaa
    46441 catggatcgt gccaaattca tctgccgcta cttcaaccaa gggttgcaag gcagttgagc
    46501 caacagccgt tatggattct ccacgatcaa tccagctagc acagcctact aaacaagccg
    46561 tcagccaaaa agcgataaga gacagagcaa gcttttttct ttttttcact gtttttctcc
    46621 tcgaaaataa ttatgaatac tgtgaatttt ttaagtagtt ctttatgagt tgacgcatga
    46681 attcttacca aatttctgcg caattgatta tttatataat ataggctata ttactctttc
    46741 ctaacctcct tttttcatat gtggataaaa tctcttgtct atcccttccc ccattgtcac
    46801 ccattatagt catttcgtgt ctctttttcc cctttttaat gcaagggaaa ttactctcct
    46861 tagatgataa tccaaaagct agaaaggtat ctcaaatctc tctactctcc cagactagtt
    46921 tacaactaaa aggaaaagat tctattttat gagaaatcta gtttacaagc ggtaagaacg
    46981 ctaataacta aacttcttgt actctttgaa aatctcttca aagcagtgtt ttgagctatc
    47041 tatggctagc ttcctagttt gctctttgat tttcatcgag tagtaaaact acatgtgatg
    47101 gcaatcaaga tatcaagaat catcctacta aaaaaatcca tactttcact ataacataga
    47161 ataagatatt tgactagcat tttcatttga atctgaggcc ttttggaaaa taatttttca
    47221 aaacatttcc agtaaccttt gcaaagccca agccattgcc tttaaccaaa acttggtacc
    47281 aaccatttgg cagactttct gccagctgaa cggtttctcc agccgcatac ttgacaaact
    47341 cttcttggcc aatttcaacc gactgttcga cctgactcgg tttcaaggct aaaccaagag
    47401 cgaaactggg ctcaaagcgt ttcttcttaa aagtacccag atgcagtcca ttgcgagcaa
    47461 tcttgagctt ccctaaatct ggcaaaagtt ctggcaagag ataaagctgg tctccaaaaa
    47521 tctgcaagat acccggtaga ttgaccttca aatggttttg ggcaaattcc tgccacaagg
    47581 caacttgttc acggctgagg ttactcttac ttgccttaaa tttaggagct ggattgttac
    47641 ccttaaactg tagatgggca acaaactgac cctctccctt aaactgatga gggtacatcc
    47701 gagccgtttc tggcaggtca ataccagcta ccattccatt gatatgctct actggcaaca
    47761 agtcaaaatc atactcttcc agcaaccaat tgacaatctc ttcgttttcc tcgggtgccc
    47821 aggtacaggt cgaataaacc agacgaccac cttcagctaa catggtcact gcatcctcca
    47881 gaatttctct ttgcaagcta gcacattgac tcggataatc taagctccaa tagtccatag
    47941 catcaggttg cttacgaaac attccttcac cagagcaagg ggcatcaaga acgattaagt
    48001 caaaatagcc tttaaagacc ttgaccaagc ggtcggcaga ttcattggtc accacgacat
    48061 ttgtcgctcc aaaacgctcc atgttttcaa ccaaaatctt agcccgtttg cttgaaattt
    48121 cattggaaac aagtagcccc tcccctgcta gataggctgc cagttgagtt gatttgcccc
    48181 ccggtgcagc agccaagtcc aagaccttca taccaggact gggttgggct acttgagcca
    48241 ccatttgagc agcaggttct tgcgaataaa ctaaacctgt agcatgctca ggcgatttcc
    48301 ctgaaacctt cccatagtgg ccccaagggg tttgagtaat ggcatcagaa aaggaaagtt
    48361 gctcttcttt taagggattg acccgaaagg ccgaaaccgc ttcctcctca aaagaggcaa
    48421 gaaaatctct tgcctcatct cctagtattt ctttatattt ttcaacaaat ccttctggaa
    48481 atttcattta agttcttttc ctttcgtaaa tataggactg aatttcctcc tgcatctcaa
    48541 gaggcaccat catgaccggc tgtctggttt gaaaatcagg agcttcacca aaaagggtca
    48601 caacccgata gcccagactt tcccctaaaa tactagctgc ggcataatcc catggttgca
    48661 gataggtgag ataggtcaac aaacgccctg acaaaatctt ggcaaaacta atggccgcac
    48721 ttccatagac acgaacacca agaaccgctc ggctcaaatc agccagcccc cattcattgg
    48781 tttccagcat accactattc cctgcaatga gaaaatctcc aagtggttta gttttaaaag
    48841 aagctagggg cctatcattt agacaaactg gaaattcccc accaccgtgg taacaatccc
    48901 ctttgaccac atcataaatc agaccaaact gtccctgacc attttcaaaa taagccatca
    48961 taacagcaaa atcttcctgc tgggctacaa aattattggt accatcaatg ggatcaatga
    49021 cccaaacctt gccctcttga accgaggctc gcagacaacc ttcttcagca caaatcttat
    49081 cctcaggata acgggacaaa atctcaccaa ccaagagttc ctgaacttct ttgtccagtc
    49141 tggtcaccaa atctgttgga gaggacttgg tttcaacacg caagtcttcc tgcatatggt
    49201 caagaatgta ctgacctgct ttcttaacaa gctctttagc aaattcaaat ttacttttca
    49261 agagaaatct ttcctttccc tttttctttg gcagcctgaa ctgctcggta aagtgaatag
    49321 ccactaaccg tttcaaattc tcgtcccaaa cgtttctctt cgctcttgct tggcacgacc
    49381 gctttgaatc ccttatagga gtccagtaac tttttagcct ctactttgcc ttcataggca
    49441 gcttcaacat cattaaaaaa agaaagcact gaagcaagtt cttcagtgct ccacgacaaa
    49501 tctagtgggt aactatactg tttgttcatt aactaatacc agctctcatt cttgcttctt
    49561 ttagttcttg cttacgataa ctacgaggga gaaaagcacg aatctcatct tcattaaaac
    49621 cgatttgcat acgcttggca tcaataataa ttggacgacg caaaagacta ggatactgct
    49681 caatcaaatg aagcaattcc gataccgaaa tactctctac atcaatattc aatttttgaa
    49741 aaatttttga acgagttgaa atgatgtcat cagtaccatt ttcggtcaag gaaaggatgt
    49801 gttgcaattc ttttcttgtt aaaggactgg tcataatatt gtgttccaca aagggaacct
    49861 tatgtttttc taaccaggtc ttagccttac gacatgatgt acagctcggt gatagaaata
    49921 gtgtaatcat gcttttctct tcttatctat actttgctac ttctattata caaaaaaata
    49981 aggcgcttga ctagggattt ttagaaaaaa agcctatttt ttcaggaaaa ataggctgtt
    50041 tgcgaacgat tgacacaatt ggatttggtt aattcactct taacgatggt tttaaacgat
    50101 atatatattt atatatgtaa attaaaaaca tctttccttt cacttcctac gacttttcag
    50161 acacagatag ccaaagaagc tttcatagag ggcaaaaaag aggaggaagg catgaagaaa
    50221 gaaggtctct ggcaaaatca taataacagg atccttggct ggatcaaaaa gccaggtatc
    50281 atctcccaca aagagaattt gatggaaaag agtaaagaat tggtcaaaac caatcaaaac
    50341 tcccccaagt ccaatcatca caggtaagac tactagagcc aggagacttt ttcgataaag
    50401 agacaaaaag tcctttttca caatcctatt gacaaagaca tagaaacttg gcagtgtcac
    50461 tagagctact agctgaacca aatgaaagag attcttgacc actgcgaaat ggtgcaaacc
    50521 agctgctgac gaacgaaaat caggcatctg taagacctga ctaaaaggat tggtcagata
    50581 attcatcaag atatgaaaat tgtattgaat ggtttctggt tttagataga ctcgattcgt
    50641 taagtttagc cactgaatct ccataggata gaaaatccaa gccagataaa tggtcagaag
    50701 gattgagagg gagaggagaa agagcataga gccccaaaag gtcaatttag ttttcatcaa
    50761 aatcccactc cgcaaggcta gaaaccacat gtgtcggtgc gattggcagg ccagctactt
    50821 cttctgcctt ggtaaaacct gtcgtcacca agagcgttgg aatgccattg tcaatcccag
    50881 cccggatatc agtcaaataa ttgtccccaa ccatgattaa ctcttcacgt tccaaaccta
    50941 agtgctcaac tgccttgtcc ataatgatgg cattgggttt tccgatataa accggcttca
    51001 ctcgtgtcgc tacttcaagc agcgtaatca gtgagccagc acctggcaaa agaccacgtt
    51061 ccgtcgggat gttgaggtca ggattggttc cgataaaatg ggcacccttt tgaatagcaa
    51121 gagttgctgt ggcaaatttt tcatagtcga cttgccaatc cagaccaact accacgtagg
    51181 caggtttttc cttgtcttcc acataaccag ccgccttgat ggcttccttg agtcctgctt
    51241 ctccgacaac atagacggtc ttttcaagcc ccaaatcatt catatagtcg atggttgcca
    51301 aagtcgctgt gtagacagtc gataggggcg tatcgatatt aaaattctga gccaacatct
    51361 ccttaacact ctctggagtg cgggttgtat tgttggttac aaagagatag ggaatgtccc
    51421 gcttttgcaa ttcatgaaca aaagtctctc cagcagggat ttggtctttc cccttataaa
    51481 tggttccgtc taaatcaatt aaatagcctt tatatttcat ctatttctcc ctaagccttt
    51541 tttatttctt gccaagtaat gattgcttgg gcattgataa ccccatcact tgtaatttca
    51601 tgcttgcttt ccagtccagt ccgttcaaca gccgatgtaa tcaccccacc tggtcgaact
    51661 tccttgacat acttgaggtt gattttcttg ggaatatagt gggtcaaaaa atccgctccc
    51721 atgacctcaa aaatccagtc taagtattta ctgttattga catgaccatt catatccaag
    51781 tcgtaaaaac gaacatggta atccttgctg atcgactctt ccaaggactc atacttcggt
    51841 ccacggataa gttttttatc aaaatcagac tggtaaggag ccacaatctc aggttcaaca
    51901 gcatggactt ttcgactgtc gcggtccatg agaacaaagg tcgccatcat atggatgagc
    51961 tcctgccccg cttcattata aatagtaaag cgacggtagc aaaaaagtcg attgtagctc
    52021 aaggcttccg tttcgatggt aatttcttcc gcaaaacgag gcaaacgaac cacctcaata
    52081 tcatattcta cgataatcca gaccagatta tagtcttcca aaatggcctt atcactaact
    52141 cccagttcaa tcgactgcat ccctgaaact tgcagtgaca gcaaaattac atctggaagt
    52201 ttgatatgac cgttcatatc agccatatca aaaggaattt tcattttcat ttgataagtt
    52261 aagcccatga tcctactcca aaataaatcg ttccgctaca gtatctccca aaaagagacc
    52321 tctctttgtc atgcgaacgt ggtcaccctc aatctgcatg aggccttgtt gaaccaaatc
    52381 tctgacaatt tctccataaa gtccagcaaa agactgtcca aatttttcct caaatcgcgc
    52441 catggaaacc ccggatttct tgcggagtcc caagaacatt tcttcttcca tttgctcctt
    52501 ttgactcagg tgatcttctg taatacaagc attgccttcc tcaaccgcac tgagataatg
    52561 acgaatggga ccatgatttt tatagcgtac tccattgaca taaccagatg cccctgcacc
    52621 aataccatag tattcagcat tgtcccagta catgagattg tgacgacttt caaagccggg
    52681 tttggagaaa ttagaaatct cataatgctc aaaacccgct cgctccagct ctgcaatgat
    52741 gtactcaaac atctccgctt ctagttcctc cttaggcaga ggcaatttcc cacgtcgcat
    52801 ccgattcata aagaccgtat ggttttctaa aatcaaacta tacaaactca tgtggggaat
    52861 atccagtcca atggctttag ccacattttc ctttacttgc tccatggtct gaccaggcag
    52921 agcataaatc aaatcaatgg agatgttgtc aaaaccagcc agtttcaggc gatcgatatt
    52981 ttcataaata tccttctcca aatgactgcg cccaatcttt ttcaacatct tatcatcaaa
    53041 ggtctggaca cctagcgaaa cacgattgac agccgaattt ttcaaaacag ctatcttatc
    53101 cgcatccaaa tcgcctggat tggcttcaat ggtcaactct tctaagacag acaagtccaa
    53161 gtttttagtc aagccattca gtaaaacctc cagttgcgga gccgacaggg ctgtcggtgt
    53221 tccaccaccg atataaaggg ttgacaactt ttcaatatca taagaacgaa actcttccag
    53281 cagatgctct aaatagctgt cgactggctg atttttgatg aagacctttg aaaaatcaca
    53341 ataataacaa atctgggtac aaaatgggat gtgcacatag gctgacgttg gttttttctg
    53401 catagtaatt attataccac aaagactaga ttccagataa aaatcaccat ccccagatac
    53461 atagtccgtc cggagatggt gatggtttat tcttctgtta tatcaatcac aatctcttct
    53521 gagtcatcaa gagcttcggc tttttcttgc cattgctcct tgagattatt taattgattt
    53581 tttgatgctt ctgtcgcttg aaaagcatag gatttagctt gagcaagtat actgtccaca
    53641 gtgatttcac ctgactcaac ctgttctttt gttttcagaa caaaatctgt agcctgctcc
    53701 ttaacttctg tcagtttttc acagacttgc tccttggcat actccggatc ttctctcaaa
    53761 tcatctagaa aatcttgagc ctgactgcaa acttgtttgc ccttatcact tgttaaaaac
    53821 aaggcaagag ctgcacctga aacggttcct aaaaggattg aggataattt acccataagg
    53881 attctccttt tttatttttt gaaaaattta cttgcaagac gaagagctga cagacttgca
    53941 ccagtcttga gtgtttttga accagctgat gaagctttct tgctcaagac acgcgcatgg
    54001 tcattgaggt ctgaaacaga tagagataaa tctgcaacag cactgaagag tggatcaatc
    54061 gtagccacct tgacattgat atcatctgcc aagacattga ccttagccaa caactcattg
    54121 gtgtgatgca aggtcacatc cacatctgaa gtcaaggttt taatcgtctt ttctgtttca
    54181 tcgatgacac gaccaagctt ttgtacagta atgatcagat agaccaaaaa gacaatcaaa
    54241 gctagggcaa caagaatata tgcaacttct aacatttagt tttcctcctc tgtaatatag
    54301 taaggggcct tctttcgatt ttgataaata acgatcatta taccgagacc gataaggaca
    54361 actgacagcc attgggacac tcgaaggccg aagaacatga gactatctgt tcgcatacct
    54421 tcgatgacca tacgaccgaa accataccaa atcaagtaaa aggccgtgat atgacctcgt
    54481 ctgagactct tccatttccg tctaaaaatc agaatcaagg caaagccaag cagattccat
    54541 agagactcat aaaggaaagt cggttgacgg tagctcccct caatatacat ctggtcacgg
    54601 ataaagccag gtagataatc cagattatcc actgttgcac cataagcttc ttggttaaag
    54661 aaattacccc aacgccccaa actttgagca atcataacgc taggcgccgc aatatctaga
    54721 aaatcccaag tattgatgag tttacggtca gcaaagatat agagcacaag agccccagtt
    54781 atcaaaccac cgtaaatggc caaaccacca ttccaaatgg caaaaatctc tcctaaattc
    54841 tgactatagt aatcaaatcg gaaaataaca tagtagagac gagctcctaa aatagccaag
    54901 ggaaaggcta ctaagataaa atctaaaata tcgtctggta tgatcttctt tctaggtgct
    54961 tctttcatgg tcaaataaac cgcaagaatc aagcctgtca caatacataa ggcataccaa
    55021 cgaatggcta ggggtcctag ttgaatagca attggatcaa gcattttgca cctcatttcg
    55081 agcgatgaga cttgtcagtc gttcgtcgaa caaacgggtc gcatcaaagc ccatttcctt
    55141 ggcacgataa ttcatggcag ctgcctcaat cacaacagag atattacgac ctgttttaac
    55201 tggaatacga atacgaggaa tggctacgcc agaaacttca agttcctctg cattgtttcc
    55261 aagacgatca aaggtcttat gcgtatcgta attttccaaa tagacagcaa gctgaacctg
    55321 tgaagaatcc ttgacagcac tcgcaccgta gagactcata acatcgataa taccaacccc
    55381 acgaatttca atcaagtgtt tcaaaatttc agctggttca ccccagagag taatctcatc
    55441 cttggcaaag atatcgacac ggtcatctgc taccaaacgg tgaccacgtt tgacaagctc
    55501 aagacctgtc tcgctcttac caattccact atctccctga atcaagacgc ccatcccata
    55561 aatatccatc aagacaccgt gcacactggt acgttctgcc aaacgagaat ccagatagct
    55621 agataactct ccagataaac gactggtagc tgcacggctg gttaaaatag caatcttaca
    55681 ttctctagca gcctttaaca tctcctctgg aaccaccaaa ccacgggcaa caatgactgc
    55741 tggtgtctca gatagaaaca tttttttcaa aacttcataa cggctgttgg aaggcatgct
    55801 gatcagataa gaccactcct tcatccccaa aagttgaatc cgctctggtg tgtagtagtc
    55861 aaaatagcct gtcatttcaa gaccaggtcg cgtaatatcc gctgtattga tttccttttc
    55921 aagcaactct ggttcaccat agacaatatc tagtctaagc ttttcaatca cttcttttac
    55981 taaaaccgac atcatttcct ccttcaaatg agttcttctc tctattatat caaattgtag
    56041 gaggaagttt cagatttttg caggctttgg agtatttatt taaaagaaaa aggactagat
    56101 ttctctagcc tttcattcca attagaattt tttacgttta cgagctactt ctgatttacg
    56161 tttacgtttt acagaaggtt tttcatagaa ttcacgtttg cgtgtttctt gaagagtacc
    56221 agctttagta accgcacgtt tgaaacggcg aagtgcgtcg tcaagagatt cattcttacg
    56281 tactactgtt ttagacattt ttttcactcc cttcaagttc gatatctatt tcattctaca
    56341 cgtaaaagga ataattgtca agcaaaaatg ctgtttaatt ttgatttttt cttccattta
    56401 tacaagagtt gaaagcgttc aatagaacat tgctttttta tttttcgagt aagctaagcg
    56461 cttcagcatc tgcgatgatg gttacatcag ggtgattttg gaggctactt gcaggtaggt
    56521 tctcagtcac tgggccagat actgttccgg caatggcttc tgctttcgac tcaccgtaag
    56581 caaaaagaat aatagacttg gcatccaaaa tgtttttaat ccccattgaa atagcttggg
    56641 ttgggacgtc ttcaatcttg gcaaagaagc gtgcattggc ttcgatagta gactggtcaa
    56701 gttctactag atgcgtttga ctgtcaaatg gagtaccagg ctcattaaag ccgatatgcc
    56761 cattgcgacc gattcccaag atttggaggt caactggatg gtcagccaaa atttggttgt
    56821 agcgttcaac ttcagcttca gcattgtcct taacaccacg aggcaagaaa ctttctttaa
    56881 atggtttttg gttgaacaag ttttcttgca tgaagtaacg ataagactgt ggattgtccc
    56941 catcaaggcc cacatactca tcaaggttga cactggttag atttgaaaaa tcaaggtcac
    57001 tctcaacaat ttccttgtaa aattcaagtg ggctacttcc tgtcgcaagt cctagagttt
    57061 gagctccatt agccaatttt tccttcaaaa tctcaaaagc aacttttcca ccttgaactt
    57121 ggttttcaac tttaataact ttcatctttt catcctccat ttctgtctat atttattata
    57181 tggtatagac caatttttgt caagtgaaaa cgatttaatt tctaaaaaaa gagcgtccaa
    57241 acttgaaacg tgttataatg gataggatta gaacttagaa agaatgaaca gatatatgaa
    57301 tacagctgat tttgatttcc acttgccaga ggaattgatt gcccaaacgc cccttgaaaa
    57361 acgtgatgcc tccaaactcc tcatcgtcaa ccgtgagaca ggagaaatgc aagataaaca
    57421 cttccactct attattaata tgctggaacc tggtgatgcc cttgtcatga acgacacccg
    57481 agttctccct gcccgcctct atggtcaaaa agtggagaca ggaggtcatg tggaacttct
    57541 cctccttaag aacactagtg gagacgagtg ggaagttctg gctaaacctg ccaaacgcct
    57601 caaggtcggt actcgtatca gctttggtga tggccgcctc agcgctgtcg ttacagaaga
    57661 attgacccac gggggacgca ttgtccgctt tgaataccaa ggaattttcc tagaagtctt
    57721 ggaaagtctg ggagaaatgc ctctgccacc ttatatccac gaaaaattag atgaccgtga
    57781 acgttatcaa accgtctacg ccaaggaaag cggctctgct gcagcaccga ctgctggtct
    57841 tcacttcacc aaagaactgc tggcagaaat ccaagctaag ggtgttcatc tagtctatct
    57901 gactctccat gtcggactcg gaacctttag acctgtttct gtggataatc tggacgaaca
    57961 cgaaatgcat tcagagttct atcaactttc tgaggaagct gctgccaccc ttcgctctgt
    58021 caaagaaaat ggtggtcgcg tcatcgctgt cggaaccact tctatccgca ccttggaaac
    58081 tattggttcc aagtttgatg ggcaaatcca agcagattct ggttggacca atatctttat
    58141 caaacctggg tatgagtgga aggtcgtgga tgccttctca accaacttcc atctgccaaa
    58201 atcaactctg gtcatgttgg tttctgcctt tgcaggccgt gaattagtct tagatgccta
    58261 ccaccatgcc atccaagaac actaccgctt cttcagtttt ggtgactcca tgtttattta
    58321 ttgagaaaga atttctctaa atcttctaat accaataaat cgctaagata ttatttcaaa
    58381 gaacatctac aattgaaact ctagctagct gtagaagacg cctagtacat tgaaattaaa
    58441 atgcttcccc ctagcttcga aaatattgcc atagattgcg ttgactctcc aaattgattc
    58501 atctatattt tatttcagct tcctatattt tcttcgctgt ttgtaaatca aaatgcaaga
    58561 cacatgagta gcaccatatt tgttactctt atctgtcctc tcaagagact attatgagtt
    58621 atttcagaat cattcactac tttgaccctg actctcctta gtctcaaaat caaagactta
    58681 tactcttcaa aaatctcttc aaactgcgtc aacgtcacct tggattatat atgtgactga
    58741 cttcgtcagt tctatctaca acctcaaagc agtactttga gcaacctgcg actagttttc
    58801 tagtttgctc tttgattttc attgagtatt aaacaaaaag tgaacaaatc tgaattctaa
    58861 tgtacagaag actaggcttg ttcacttttt tatagtcgct ataagatgac cttatctata
    58921 gctttttata tataattata tataattata tattcagaca tactattatc aattttgtcg
    58981 cagggaggaa tctgttaacg cacccattca ccattatcat tgactctata gccatctata
    59041 cttgtattga ccgctaactc acccgatgca tttacataat accatttacc accaacttgg
    59101 aaccattgat tgactttcat agaaccgttg ctgttgaggt agtaccatga accattaact
    59161 tgtacccaac ctgttgccat ggaaccatca gtattataaa aataccacat accattttct
    59221 tgtttccagt ctgttgttgg agcaactgct ttagctggtt ctactgctac atctgttcct
    59281 tggttagatg taacagatac aggatacgaa ggaatagatg attgctcagg aacaacaact
    59341 ttttcaggtt ctctcgtccc tctccttata cgtcttttta ccatctcttt agtaatttga
    59401 cgagaagtag tttcttcaat tgttccatca cgttcatcta cagtatagat tgtagtaaga
    59461 gtaatttacc aatttctcct acttcttcta cttcttgact tttatcaaga gttgggtcat
    59521 cgagatattc tgtttcgatt ggaatttctt ggacaagaac ttggggcttg gttccttttt
    59581 taacaactct tgtttgagag tcttcttttt gacttaaagt actctcagtt acttgtccac
    59641 tctttccatc tacatcataa gttatcgttg taactgtttt cccattcttt cctagagtaa
    59701 tctcttgctc ctgtcctgca gaaaggtcat tgtctgcttc atatttagta gcacatggaa
    59761 caagaacttc ttcaaccttg cttttagctg gaactttgat aactgtatcc gtggcttgtt
    59821 ttctatcaac agtaacctgt tcggtaacat gaccagtctc tggattaaca tcgtaggtcc
    59881 ttgtcgtagt tacatagcca tcctctccat caattgtaac aggattttca ctacggtctt
    59941 ttgtttcatc tttttcataa cgaattcgcg tacttgaaat tttcttggtt actaccttag
    60001 gtttagtcgc tacttttaca ataatacccc cattgtcagc gtcatcatac tctattccct
    60061 cttctttatc tctagtatca tctctgacat attgaatccc atcagcagca tgaacaaaac
    60121 ttgtattcag attcctccta aaaataaagt tagcccgatt accgcagaac caaaaatctt
    60181 tccgagttta cgtaatgcat agcgcttatt agtattagat tttgccatta catcctactt
    60241 ctagtatagc atcttttcta tcaaacgtta aacaatatac tttatatata aaatagactt
    60301 agaatgatat attgattatt gaactaacac tttaactata tcgtaatcaa tctcatatat
    60361 aaaggattgc agacatctta tctaaataca tgctaatata tttagataca aacattccaa
    60421 cttgataatt ttcactcatc tttcatcatt ccttatacaa ctatgcagta taaatagaat
    60481 agttttctca tcagaatgag actattttaa tattagataa tttgatagaa aaaacatgaa
    60541 taaaattggt tgaatttaat tccctaactc gatagaggat tctattttaa atttaacaac
    60601 agctaggatt acactataaa aagtacatta ccacctgttc ggctggagaa tctagtcgat
    60661 tagctccctc ttagttacaa gcttaaagcg attttttaac taatactaaa ccagattaaa
    60721 gaaggaacta gaacatctgt cttgtactaa tcaatatgct attttaaagt ttcaaataaa
    60781 cttggaatgc taattattat atttacaata aatagttgac ttcgatagaa acagaaaata
    60841 gaattcaaca ttcttcccta cttataccat aataaataag atccgcaaga tagccttttc
    60901 tctctacacc attaacaatg gtttgtagat aggacattcc agccttttcc atgacacgac
    60961 ctgaagctgg gttgagactg gcataacgtg ctctgacctt ttgaaaacct gcttgagtaa
    61021 aacaaaagtc caagatagct ttcaaagtct ctgtcatcat accatttccc cagtaagcct
    61081 tgcctaacac atagccaatt tcacagctta aatcagcctc gtctatctta acgatgctga
    61141 tatctcctat cacttgctct gggttttctt ttagacaaat ggcccattta taatagttga
    61201 gattagtata ggaagcaacc caattgcgaa tcgagtttcg agtgatttcg atatcaggat
    61261 ggggatccca ggtaacatag gtcagattct cagcagatga agcccaattt tgaaacatgg
    61321 cttctgcatc actctccaca aatcttctta aaatcaaacg ttctgtctgt aatatttgcg
    61381 taccgataga tttcatgatg ctacctcact tccttatttc tatgctcgta gtaaaaaggc
    61441 tcaactcagt aacagctaga aaacgagttt gtcttctaat ttcttgacgc agtcgctgcc
    61501 tggtttgaaa tgcgctctag caagcttttt caaacctagt catcgttgca ggggtaagac
    61561 gatgaaatcg aactcttcga gttaccgaat ctgcctcatt ttcttatttc taaactcggg
    61621 gttcaagatc ccccagacct tctctcactt tcttatttca agatgagagg ctgaaaacct
    61681 ccagtggagg tttttcttgc tttttaattt cagggcaaga agctaaaaag gtccccggac
    61741 cttcttcgct ttctcatttc taggctcagg gtaaaaacct ccaatggagg tttttactcc
    61801 caaaagtcat caaatacagt gatgggtaag tggcgtttgt gttggccttt gtgccaccag
    61861 ttttcaatgg tcgcttgagc ttctgggctg attgttttgc cttctaggta gtcgtcaatc
    61921 tctgcgtagg tgactccaag tgcgacttcg tcagctaggc ctggtttatc ttcttctagg
    61981 tctgccgttg ggattttttc ataaagggct ggctctgcgc caagtttctg caagagctgt
    62041 tttccttggc gtttattgag gcggtaaaga gggagaatat ccgcaccgcc gtcaccaaac
    62101 ttggtaaaga aacctgtgat attttccgcg gcgtggtctg ttccaatgac cgctccgcta
    62161 tgggaaccag caagggcata ctgagcaatc atacggcaac gtgccttgat attccccttg
    62221 ttgaagtctg aaacaggact acctgtcgct tcaactgcag ctgtcatggc atcagctgat
    62281 tccttgatat tcacaaccaa gctgacatct ggctggatga aggctagggc tttttgagca
    62341 tctgcttcat cagcttgcac tccgtatggc aggcggacag cgataaattt gtagctgtcg
    62401 tctcccgttt cagctcgcag ttcttccata gctaattgcg ccaaacgccc tgccaaggtt
    62461 gagtcttgtc ccccagaaat ccctagtaca aaggttttta ggaagggatg ttttttcaga
    62521 tatcttttta agaaatcaat agaacgacgg atttcttcct gggcatcaat cactggtttg
    62581 acacccagct cttggataat cgtttcttgc aaactcattc ttcttctcct tcaccaaggg
    62641 cttccttgcg catcttatca atcaagtcca tcttatcttg ccatacatca cgcgccaaat
    62701 ccactggata gtgctgcgga ttgagcacac gcttatactc atcccacaac ttgtcaaatt
    62761 ccttacgggc ataatcctga atgtcagtca aactaggcaa gttgtaaact aatattcctt
    62821 ctttgaagat atccaccaag agaggaacgg catcaaaatt acgaaccgtc ttcttgatgt
    62881 atgtataggt cggatggaac atcttgattt ctgtcatgtc gctaatatcc acaccatcat
    62941 aagtgatata gtcgccttct gacttgcctt tttcacgact ggtaatgcgc cacacctgct
    63001 tcttacctgg cgtagagact ttttccgcat tattagacag cttaatcgta ttgcgcatct
    63061 gaccagtttc atcttcgatt gcaacaatct tgtaaactgc accaagagct ggctggtcat
    63121 aagctgtaat cagcttggta cccacgcccc agacatcaat cttggccttt tgcatcttga
    63181 gattgaggat ggtattttca tctagatcat tagaagcata aatcttagcc tctgtaaatc
    63241 cagcctcatc cagttgctga cggactttct tagaaatgta ggcaatatcc ccagagtcaa
    63301 tccgcacacc cataaagtta atctgatcac ccagctcacg cgccacctga atggcagctg
    63361 gtacaccgat gcgaagggtg tcataggtat ccacaagaaa gacacaattt ttgtgggtcg
    63421 cagcgtaagc cttgaaagct tcatagtcat tgccataaac ctgtaccaag gcatgggcat
    63481 gggttcccaa aacaggaatg tcaaagagct tacccgcacg cacgttgctg gttccattgg
    63541 cgccaccaat cacagctgcg cgtgttcccc agatggccgc atccatttct tgagcccgac
    63601 gtgtcccaaa ctccatcaag ggttcatctt cgataaccga acgaatacga gctgccttcg
    63661 tcgccaccaa ggtctggtag ttgacgatgt tcaaaagagc cgtttcgacc aactgacatt
    63721 gggctagagg tccttccacc tgcacaatcg gttcattagc aaaaaccaaa tccccttctt
    63781 gggcagaacg aacggtcaac tccaacttga aattgcgaag gtaatccaag aacgccccat
    63841 gataaccaag cgactccaaa taggctatat cactatctga aaaacgcaag tcttcaagat
    63901 agttcacaat tctttccaaa cctgcaaaaa ccgcatagcc gttcttaaaa ggctgttggc
    63961 ggaaatacac ctcaaagacc gccttcttat tgtgaatccc ttggtcaaag taaacctgca
    64021 tcatgttgat ctggtacaag tccgtgtgca atgtcaaact atcatctgga tacatacttt
    64081 tcctacttcc ttagctagaa acccatgaaa attttcaaga actttcatgt attccaataa
    64141 attagtacta ttatatcaca ttttagctgg attgagaaaa gagtaacaag ctattctcca
    64201 ctctccaatt catccatatc ttgttcaaat tttttctgag cccattcgcc atagctctta
    64261 agaccaagat tgccaataaa gacccacgga aggtaaatga cataagtaat gacccaagca
    64321 gacaggtatt taaaattcaa aggattgtgc tgataaattt ctatgttgaa ttgataattc
    64381 tgcaacatca aaagagccgt aatagccaag gttaggaaaa aacaacccaa aatcgtaaaa
    64441 tgaaaacgac tatagtaggt cactcccaga taacgggcac ggttaaaaaa gtaaaatgtc
    64501 cctatgatga taacgattag cagcatatta gaattaaaaa ggcttggtgc taatactgaa
    64561 atgatataag ataggagcga caaagcgatg cagatataga aactttcaga gcccgcttta
    64621 ttgatcagtt gttcttctct ttcgtctagt aattgataat aataaaatct atttttcatc
    64681 ttcttcctcc caaaatagtt ggtctagggt tttccctaaa catctgcaaa tagactggca
    64741 gagcgagaga ctgggattgt attttcccgc ctctatcaaa ccaatagtct ggcgtgtcac
    64801 cccgacagcc tctgccagtt gaccttgtgt taaatcacgc tctacccgag ctaattttaa
    64861 ttttaaattt ttagccacct tcgtcctcct tatagtttta atactcatct actctttaaa
    64921 aatccaaact aacacaagct gtcagctatt tattataacc ccattaattt gcactccagt
    64981 attatcttca gccgaaaatc agtattttag gttgtttgaa ataaacttcc tagtttactc
    65041 ttttattttt atttttattt agcattagct ttagtaggat cttttaaacg agtttttatt
    65101 gcttatattt cttgtttagt ttagttggtt tcatatcact attatataat gcttttggaa
    65161 atgagcctag tataagtcaa aaacgtgttg aatagtgaaa gcaggagcat accaatatga
    65221 ctgatttttt actgtatttt aggtatgcaa aataatacta aatgaaacta aaatgaatac
    65281 ttcttgcttt tcttgagagt gaaaaagtac aataaaatga ttgtagtgat tgccacaaga
    65341 tactgactaa tagacatcgt atttgaagcc acaaatacag gtaaagaata aaaaggaaga
    65401 agttcaattg ctttgataaa caaatcatta ctagtattca tcatcgtatt taatatatta
    65461 gggataaaga gtagtaaagc tactaaaata ccaagccact tttcaatttg tataaataga
    65521 aaatgtaaga taatagaaaa taaaacagga ctaacaaaat tgtattgact acaatagcta
    65581 ctataaaatc taatcctaaa tcacctggaa tacccaattt acttactatg actagtaaca
    65641 caatgccatt caaagcataa ataatggaag ctgagatggc atatagaaaa ttagacaaga
    65701 cgtaaatcat ccgattcttc tgttgattat aaaacaaact taccgtattg tgggaaaaat
    65761 ctctactaat gactcgatta gcatgtataa cagccattaa cataccgagc atagcataaa
    65821 agtttgaaat atgcttaata ccaagcgatg tcccttaagt ctttagaata aagaatgtaa
    65881 gcaatatcgg cggtataaac gagataagca aactgagata tattacgcct gatgaatata
    65941 aatttcttat attttcctta agaaaattca atttatttaa tttcatgatt ataaccttcc
    66001 tttattttga acgatttagg taaatatctt taagcgtctc ttttttagtt tcaaaatcaa
    66061 ctactttaat agagttttcg ttaaaaaatt taaaaagctc actactttga atattcccag
    66121 acatagtaat tctcaatccc tcttcctgaa caatatcccc aaattcttgt ttggtaatga
    66181 aaatgtctct atctgtagct gatgaaaaag ctatctcaaa caagaaatta tgactatctt
    66241 ttcctacttt ttgaaatgtc aaaagcccgt tctccaagaa aagaactctc tcacaaattt
    66301 cttcaatgtc ttctaattta tgactcgata ttaaaattcc cacgttttca tttaaagcta
    66361 atttttttag aaccgctaaa actatttgtg atgattcaat atctaaacca ttagtcggtt
    66421 catctaaaat caatatatca ggttccgtaa cgagagttag aagcaaagct aatttttgtt
    66481 ttgtacccaa agaataggtc tttacttttt tattaataga ctgagtcaaa tctaactctt
    66541 ggatcaaact tctaaatctt tcttgattgt agtcaacacc atataaattt gacaaatatt
    66601 ttaaattctc taaacctgtt ttagataaaa ataattttgg ttcttcgatt aaatacccaa
    66661 cattatcact gcttataata ttacctgaag tcggttgatt attttgaaca agaattttca
    66721 tcaacgtact cttaccaact ccatttctcc ctactagacc aacaatttca cccttgttaa
    66781 gtgcaaaatt tatatctttg agaatgattg aatgaccata atgcttactt acatttttat
    66841 tcctacatta atattcatgg tttactcttt gattcttatt gagtttaaaa agttgcgcct
    66901 ctttttaaca atattatatc atatatctaa cttgatgcaa tatataaatg tcattttgta
    66961 aaattttatt aacaaaaaac tctctacaac aaaataatag agagttttct gcttattaat
    67021 ttttttcaaa atatctaaga catcgcttct ttgtttaatt tgataggctt tgatacctaa
    67081 gctccgactt tatcacttaa aaaataaaaa agacagctaa tctcttgcgc atcaagggat
    67141 ttcgccattc tttaatctcg tttaagtgtt gtatatggag ctttgcataa ttttttaaaa
    67201 ctaaacaact acattaagca cgaactgtga cgctggttcc atcttcctta taaagattga
    67261 taagcccctc tttaagagct cgaaccatgt ctcctgattg aacgggttgt gggac
//
LOCUS       DAVBCB010000004        64203 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVBCB010000004 DAVBCB010000000
VERSION     DAVBCB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 64203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64203
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.004"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(179..526)
                     /locus_tag="VQU28_000226"
     CDS             complement(179..526)
                     /locus_tag="VQU28_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2475657.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(637..2412)
                     /gene="spxB"
                     /locus_tag="VQU28_000227"
     CDS             complement(637..2412)
                     /gene="spxB"
                     /locus_tag="VQU28_000227"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HET2475658.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2619..4862)
                     /locus_tag="VQU28_000228"
     CDS             complement(2619..4862)
                     /locus_tag="VQU28_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2475659.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQSLHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4872..5243)
                     /locus_tag="VQU28_000229"
     CDS             complement(4872..5243)
                     /locus_tag="VQU28_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HET2475660.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QVGEFGFACGMNMMHGKMIVE"
     gene            complement(5254..5649)
                     /locus_tag="VQU28_000230"
     CDS             complement(5254..5649)
                     /locus_tag="VQU28_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HET2475661.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5833..5931
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6015..6806
                     /gene="thiD"
                     /locus_tag="VQU28_000231"
     CDS             6015..6806
                     /gene="thiD"
                     /locus_tag="VQU28_000231"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2475662.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEGMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7015..7647)
                     /locus_tag="VQU28_000232"
     CDS             complement(7015..7647)
                     /locus_tag="VQU28_000232"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2475663.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARKVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPV
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7640..8443)
                     /locus_tag="VQU28_000233"
     CDS             complement(7640..8443)
                     /locus_tag="VQU28_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2475664.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAASYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MSFLVTGPKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8443..8967)
                     /gene="thiW"
                     /locus_tag="VQU28_000234"
     CDS             complement(8443..8967)
                     /gene="thiW"
                     /locus_tag="VQU28_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HET2475665.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERID"
     gene            complement(8972..9664)
                     /gene="tenA"
                     /locus_tag="VQU28_000235"
     CDS             complement(8972..9664)
                     /gene="tenA"
                     /locus_tag="VQU28_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HET2475666.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9675..10325)
                     /locus_tag="VQU28_000236"
     CDS             complement(9675..10325)
                     /locus_tag="VQU28_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HET2475667.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAIFPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAYGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10327..11712)
                     /locus_tag="VQU28_000237"
     CDS             complement(10327..11712)
                     /locus_tag="VQU28_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475668.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQMI
                     AQVSSLPYSGQVLIDGSEVSQLSIVERVQTVGILLQNPNHQFTMESLFEELVFTMENI
                     GYHLQEIDSKIAEVVQQCRCKDILHRLIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELVYDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGSITWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11713..12273)
                     /locus_tag="VQU28_000238"
     CDS             complement(11713..12273)
                     /locus_tag="VQU28_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2475669.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGFVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12535..12623)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12832..13461)
                     /locus_tag="VQU28_000239"
     CDS             complement(12832..13461)
                     /locus_tag="VQU28_000239"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HET2475670.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFGTQSKDDAGGTIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNMDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13463..14245)
                     /gene="thiM"
                     /locus_tag="VQU28_000240"
     CDS             complement(13463..14245)
                     /gene="thiM"
                     /locus_tag="VQU28_000240"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HET2475671.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVMIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            complement(14488..15156)
                     /locus_tag="VQU28_000241"
     CDS             complement(14488..15156)
                     /locus_tag="VQU28_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HET2475672.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVDKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15371..15459)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15713..16849)
                     /gene="lctO"
                     /locus_tag="VQU28_000242"
     CDS             complement(15713..16849)
                     /gene="lctO"
                     /locus_tag="VQU28_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HET2475673.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17474..18964)
                     /gene="lysS"
                     /locus_tag="VQU28_000243"
     CDS             complement(17474..18964)
                     /gene="lysS"
                     /locus_tag="VQU28_000243"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HET2475674.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKTIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19092..19238)
                     /locus_tag="VQU28_000244"
     CDS             complement(19092..19238)
                     /locus_tag="VQU28_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HET2475675.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAGDTF
                     TSFQ"
     gene            19464..20132
                     /locus_tag="VQU28_000245"
     CDS             19464..20132
                     /locus_tag="VQU28_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006531263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2475676.1"
                     /translation="MVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHYRILV
                     LAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMAESFR
                     SGLEAISQTQQEIGLAIGLTPLQVFYYVVLPQATAVALPSFSANVIFLIKETSVFSAV
                     ALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRLRHAGFGNPST
                     LSRK"
     gene            20092..20769
                     /locus_tag="VQU28_000246"
     CDS             20092..20769
                     /locus_tag="VQU28_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2475677.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIEISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLDFTRIMPQLVLLFIVYFGLARNFNINISGETSAIIVFTLW
                     GAAEMGDLVRGAITSLPKHQFESGQALGLTNVQLYYHIIIPQVLRRLLPQAINLVTRM
                     IKTTSLVVLIGVVEVTKVGQQIIDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTH
                     LEKHWRN"
     gene            20770..21527
                     /locus_tag="VQU28_000247"
                     /pseudo
     CDS             20770..21527
                     /locus_tag="VQU28_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21539..22400
                     /locus_tag="VQU28_000248"
                     /pseudo
     CDS             21539..22400
                     /locus_tag="VQU28_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765255.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(22480..23127)
                     /locus_tag="VQU28_000249"
     CDS             complement(22480..23127)
                     /locus_tag="VQU28_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475678.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23124..23822)
                     /locus_tag="VQU28_000250"
     CDS             complement(23124..23822)
                     /locus_tag="VQU28_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475679.1"
                     /translation="MGKLSSHMWRLNKIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDVGGDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(23832..24584)
                     /locus_tag="VQU28_000251"
     CDS             complement(23832..24584)
                     /locus_tag="VQU28_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475680.1"
                     /translation="MKQFVQFYKKDFLSVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTATLFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLTLLSIIIPLTVFSVHRNWRRV"
     gene            complement(24584..25348)
                     /locus_tag="VQU28_000252"
     CDS             complement(24584..25348)
                     /locus_tag="VQU28_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475681.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQLLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(25444..25847)
                     /locus_tag="VQU28_000253"
                     /pseudo
     CDS             complement(25444..25847)
                     /locus_tag="VQU28_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(26027..26659)
                     /locus_tag="VQU28_000254"
     CDS             complement(26027..26659)
                     /locus_tag="VQU28_000254"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HET2475682.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYRELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(26693..27394)
                     /gene="pyrF"
                     /locus_tag="VQU28_000255"
     CDS             complement(26693..27394)
                     /gene="pyrF"
                     /locus_tag="VQU28_000255"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HET2475683.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQNWN"
     gene            27715..27807
                     /locus_tag="VQU28_000256"
                     /pseudo
     CDS             27715..27807
                     /locus_tag="VQU28_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516271.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(28129..28842)
                     /locus_tag="VQU28_000257"
     CDS             complement(28129..28842)
                     /locus_tag="VQU28_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2475684.1"
                     /translation="MLKRFLALVWLRCQIILSNKSILLQVLVPFAFTYFYKYLMETQG
                     KVNDQQALVLLMMCLPFSLALAVGSPITIILSEEKEKYNLQTLLLSGVKGSEYILSTM
                     FLPFLLTFVIMGTTPLILGVTIVHTFNYITIVLLTSLSIILFYLLIGLTAKSQVVAQV
                     ISLPAMILVAFLPMLSGLDKTVAKITDYSFMGLFTKFFTKWEGFSWNKTLIPNLTLLI
                     WIVLLLTLITITIRKKKIS"
     gene            complement(28835..29530)
                     /locus_tag="VQU28_000258"
     CDS             complement(28835..29530)
                     /locus_tag="VQU28_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475685.1"
                     /translation="MTGQFLADKGQSIILGQKSQNLTSGELKRIGLVSDTSGFYEKMS
                     LYNNLLFYSKFYNISKSRVDNLLKRVGLYDSRKMVAGKLSTGMRQRMLLARALINKPA
                     VLFLDEPTSGLDPTTSRTIHELILELKTAGTTIFLTTHDMNEATLLCDYVALLNKGKL
                     VEQGAPSELIQRYNKDKKIKVTDYNGNQITFDFTSLEQVSQADLENIFSIHSCEPTLE
                     DIFITLTGGKLNA"
     gene            complement(29870..31202)
                     /locus_tag="VQU28_000259"
                     /pseudo
     CDS             complement(29870..31202)
                     /locus_tag="VQU28_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812020.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
     gene            complement(31186..31984)
                     /locus_tag="VQU28_000260"
                     /pseudo
     CDS             complement(31186..31984)
                     /locus_tag="VQU28_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824573.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M50 family protein"
     gene            complement(32774..33973)
                     /gene="divIB"
                     /locus_tag="VQU28_000261"
     CDS             complement(32774..33973)
                     /gene="divIB"
                     /locus_tag="VQU28_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HET2475686.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEEPES
                     KEKEEQDKKLSKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(33983..35041)
                     /locus_tag="VQU28_000262"
     CDS             complement(33983..35041)
                     /locus_tag="VQU28_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HET2475687.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVCWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VAKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQLENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(35043..36395)
                     /locus_tag="VQU28_000263"
     CDS             complement(35043..36395)
                     /locus_tag="VQU28_000263"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HET2475688.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(36499..37140)
                     /locus_tag="VQU28_000264"
     CDS             complement(36499..37140)
                     /locus_tag="VQU28_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475689.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNCVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTTSLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(37142..39163)
                     /locus_tag="VQU28_000265"
     CDS             complement(37142..39163)
                     /locus_tag="VQU28_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2475690.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(39234..39533)
                     /locus_tag="VQU28_000266"
     CDS             complement(39234..39533)
                     /locus_tag="VQU28_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2475691.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEASAGKTSYAWIWTKWGEQVAFYCDYD"
     gene            complement(39899..40153)
                     /locus_tag="VQU28_000267"
     CDS             complement(39899..40153)
                     /locus_tag="VQU28_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HET2475692.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(40173..42014)
                     /gene="typA"
                     /locus_tag="VQU28_000268"
     CDS             complement(40173..42014)
                     /gene="typA"
                     /locus_tag="VQU28_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HET2475693.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            42176..42901
                     /locus_tag="VQU28_000269"
     CDS             42176..42901
                     /locus_tag="VQU28_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HET2475694.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNELLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            complement(42941..43168)
                     /locus_tag="VQU28_000270"
     CDS             complement(42941..43168)
                     /locus_tag="VQU28_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475695.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(43257..43637)
                     /locus_tag="VQU28_000271"
     CDS             complement(43257..43637)
                     /locus_tag="VQU28_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2475696.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(43637..43906)
                     /locus_tag="VQU28_000272"
     CDS             complement(43637..43906)
                     /locus_tag="VQU28_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HET2475697.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            43970..44878
                     /locus_tag="VQU28_000273"
     CDS             43970..44878
                     /locus_tag="VQU28_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2475698.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(44932..45687)
                     /locus_tag="VQU28_000274"
     CDS             complement(44932..45687)
                     /locus_tag="VQU28_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2475699.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(45708..46637)
                     /locus_tag="VQU28_000275"
     CDS             complement(45708..46637)
                     /locus_tag="VQU28_000275"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HET2475700.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(46652..47275)
                     /locus_tag="VQU28_000276"
     CDS             complement(46652..47275)
                     /locus_tag="VQU28_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HET2475701.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFSHQLVFEESGKIYMKPVKDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(47268..48506)
                     /gene="hflX"
                     /locus_tag="VQU28_000277"
     CDS             complement(47268..48506)
                     /gene="hflX"
                     /locus_tag="VQU28_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HET2475702.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(48499..49383)
                     /gene="miaA"
                     /locus_tag="VQU28_000278"
     CDS             complement(48499..49383)
                     /gene="miaA"
                     /locus_tag="VQU28_000278"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HET2475703.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKCFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            49504..49674
                     /locus_tag="VQU28_000279"
     CDS             49504..49674
                     /locus_tag="VQU28_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HET2475704.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(49722..50561)
                     /locus_tag="VQU28_000280"
     CDS             complement(49722..50561)
                     /locus_tag="VQU28_000280"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HET2475705.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPIITLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(50658..51617)
                     /locus_tag="VQU28_000281"
     CDS             complement(50658..51617)
                     /locus_tag="VQU28_000281"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HET2475706.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(51744..52742)
                     /locus_tag="VQU28_000282"
     CDS             complement(51744..52742)
                     /locus_tag="VQU28_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2475707.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGAFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGNYYLKADGKMAQSEWIYDSSYQAWYYLKSDGSYAKNAWQGA
                     YYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGNYYLKSDGKMAKGEWVYDA
                     TYQAWYYLTSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTA
                     SSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDG"
     gene            complement(52792..53409)
                     /locus_tag="VQU28_000283"
     CDS             complement(52792..53409)
                     /locus_tag="VQU28_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2475708.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQHLAQETEPFVLIGLSLGAALTLELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKTLMVGVSEELKTLDLTDIAGTCSYPTLLICGSKDKPNL
                     SSMKALHRLLTDSQFQIIPDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(53410..55131)
                     /gene="pabB"
                     /locus_tag="VQU28_000284"
     CDS             complement(53410..55131)
                     /gene="pabB"
                     /locus_tag="VQU28_000284"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HET2475709.1"
                     /translation="MHRKTVIDFRTLGERYTFAQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSPIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLTQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(55200..60887)
                     /locus_tag="VQU28_000285"
     CDS             complement(55200..60887)
                     /locus_tag="VQU28_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2475710.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEETPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKSEEKVAVKPESQPSDKPAEES
                     KVEPPVEQAKVPEQPVQPTQAEQPSTPKESSQEDNPKEDRGAEETPKQEESTPDTKAE
                     ETVEPKEETKTAKGTQEEDYVGEALVPEQLPEYKVTEGTLVEESTTELDYKTETTEDP
                     TKYTDEETVLRNGEKGSQVTKTTYKTIGGVKTDQVLSTSTEVTKEPVNQQVIRGTKPI
                     EGTTVETREEIIPFETKEQEDDTLKRGTRQVTQEGVNDKKQITETYKTIRGEKTSDAP
                     TITETVIEKPQDKIIKNGTKELEKPTLEWAKTDKDVLKKSATASYTLTKPAGVEIKSI
                     KVALKDNTGTVVKEVTVEENNLQATLDNLKYYQGYTLSTTMVYDRGNGEETEILEDKE
                     VQLDLKKVEIKDIKETRLMKVDENGNETDSSLLETVPENLTSYYLKITTNHNKVTRLA
                     ITNIEEVTEEETSLFKVTASAPDLIQRDSNNSLSQTYTYYIEKPKASQNNVYYNFKDL
                     VDAMQKNPNGEFKLGSDLNATNVPTPSKSYVTGKFTGHLTSVDGKHFSIHNTARPLFD
                     RVEGGSIKNINLINVNINMPWANQLASLAQTVKNATVENIKVTGTIVGNNNVAGILDK
                     LDDAGIMTNVAFIGKVQSVGDKGWNLAGIASEIYRGNLNKAYADVELKGLNTRAGAIV
                     GHLAHDFNPRDVGAWSNVRNSVAKGTIDVARGDVGGLIGKDWPWGTIDNNISLVNVKR
                     GDIFFGSNDMDDEFVSNWIKPNNATVIGVSSGDKTHKQSSKIKEISQVEADKKIKDMG
                     ITADTFEVTEHLVDKLNRRILRDEEYKTIQDYNANRELVYRNVEKLQPFYNKEWIVNQ
                     GNKLTTDSPLMNKEVLSVTAMKGNDFITDLTDADHIMVHYADKTKDIFTISPKDSQVK
                     QVKEYSVAELGEVVYTPNMVVKDRADLISAIEGILSPIELQSDPIYQRLGRTGDNRVN
                     AIKNLYLEESFKEVKDHLNTFVTKLVENEDHQLNTDEAAKRALIKKIEDNKAAVLLGL
                     TYLNRYYGVKFDNFNIKELMLFKPDFYGKNVSVLDRLIEIGSKEDYIKGSRTQESFRE
                     VVAKNTLSGNLHDFLTYNMKLFTNETDINVWFKKAIENNAYVVEQPSTNPAFANKKYR
                     LYEGLNNGQHGRMILSLLNLKDAHLFMISTYNTISFSSFEKYGKDTEEKRESFKSEIN
                     KRAKEQVNYLDFWSRLATDNVRDKLLKSQNVVPTPVWDNHGAPGGWPDRFGHRKDKPD
                     YAPIREFFGRIDKYHDYKYGYGAYAYIFADPQPMDAVYFVMSDLISEYGTSAFTHETT
                     HVNDRMAYLGGHRHRQGTDLEAFAQGMLQTPAEHGHQGEYGALGLNMAFERSNDGNQW
                     YNPDPTKLQTRDQIDHYMKNYNEAMMMLDYAEAEAVLPKVKGDNSKWFKKIDRETRRP
                     MDRNKLSGPHQWDKVRDLIDAEKTTNLETIDDLVNNNFMTIHGNPGNKVFHPEDFGTA
                     YVNVNMMAGIYGGNTSQGAPGSLSFKHNAFRMWGYYGYEHGFIDYVSSKHQGAANKEN
                     NGLLGDDFIIKKVSNNQFSNLEDWKKQWYGEVLTKSKKGFAPIEINGTQISTYEQLKT
                     MFDEAVTKDLTELNDSSIKDKYRNTVVLKSKVFKALLKNTDGFSGDLFTAPQA"
     gene            complement(60909..61472)
                     /locus_tag="VQU28_000286"
     CDS             complement(60909..61472)
                     /locus_tag="VQU28_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HET2475711.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGVLFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(61588..63279)
                     /locus_tag="VQU28_000287"
     CDS             complement(61588..63279)
                     /locus_tag="VQU28_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2475712.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(63276..64013)
                     /locus_tag="VQU28_000288"
     CDS             complement(63276..64013)
                     /locus_tag="VQU28_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2475713.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
BASE COUNT        19328 a        13922 c        11332 g        19621 t
ORIGIN      
        1 aggaggtgat aaagtccatc gttaatcgga gatggaatag aatgcttttt gaaagcagat
       61 gagtattata tgcaatttct ttatataaaa agaccggatt gctccgatct ttcaatagtt
      121 catattctca atttctattt taaaaatagc taaggttaac gtcaaatgac tacgcgacct
      181 atttcatacg ataaaaatca agcactagac cagcaggtcc ttgaactaat aaggactctg
      241 ttccccaatc ggttacagtt ggtccgtgta aaacctttat accaagctca ttcaaccgtt
      301 tgtagttctg gtctacatcc tcaacctcga tatgaataat gattcctgac tgaaagtttt
      361 ccaaaggaac caaatgattt tgtgacaaca taaggcagtg actaccaatc gtaaactgag
      421 caaaaccatc atcagcataa tctgcctttt tatccaaaat atgctccaag tcagcacaga
      481 cttggggaac atttgaaacg ataatatcta attgatttaa attcatttac tctcctccat
      541 aaaaagaccg aattgctccg atcttttaaa gttctgctct atgaaaatca aagaataaag
      601 tctacaagtt tcatatttga ttttcggcga gaggaattat ttaattgcgc gtgattgcaa
      661 tccttcttct tccaagaaga gacggaatgg tacgagttct tctgcttcgt atttttcctt
      721 gaaggctttg atagcttctt ctgagtgaag ttttggatcc aattcaagta cttctactgg
      781 aagtggacgg tgttgagtga tgcgagcatc gatgacaaca gttttacctt ctttgttcaa
      841 tttaacagct tctgcaacaa ctgcatcgat gtcttcgata cggtcaactg tgaatccaac
      901 agctccttga gcttccgcaa ttttagcgta gtcagcgttt gtgaagtcta caccaaacaa
      961 gtgtttgttt gtatcttcgt atttgttctt gatgaagccg tactcagcat ttgagaagac
     1021 aaggttgata actggaaggt cgtattgaac gtttgtgata acgtctgggt agcacatgtt
     1081 gaatgctcca tcacccatga tgttccatac ttggcgatct ggattgtctt tcttagcagc
     1141 gataccacca ggaagggcaa tacccattgt cgcaaagagc ggagatgtac gccacatatt
     1201 cttaggtgtc atgtgaaggt gacgagtaga tgtttgagta gtgttaccta cgtcgattga
     1261 gtagatagcg tcttgatcag catgtttgtt gattgcattg taaacttgat acaattgcaa
     1321 ttcaccctca gttttacctt cgagtttgtt catgtaatca cgccagtttt ggttgttctt
     1381 aacgtttgca cgccaccatg gagttgattc aactgggttt actttgtcaa ggatagcttt
     1441 agctgcttga ccagcatcac caaggattga agcgtcaagg gcatgacgtt taccaagttt
     1501 gtaagggtcg atatcgactt ggatgaattt ttcagtgttc ttgaatgctt cgtaaacttc
     1561 agcaaatggg aagtttgaac caaggaaaag aactgtgtct gcttcaaaga ccacttcgtt
     1621 ggctggtttc caaccaacac ggtaagcaga acctgtcaaa ccttcatagt tccattcgaa
     1681 agcttcaaag tttttaccag ttgtgatgat tggtgctttg attttacgtg acaattcagt
     1741 aatcacttca ccagctttaa caccaccaaa tccagcatag ataactgggc gttcagcatt
     1801 gttcaagatt tcaacagctt tgtcgatttc aacttcgttc aaagcaggag cgatgaatga
     1861 gcgttcgtat gaacctgaac cgtagtatga gttttcgtcg atttcttgga aaccgaagtt
     1921 tactggaatt tcaacaacag ctggaccttt tttagaaatt gcagcacggc aggcttcgtc
     1981 aattactttt ggcaattgct cagcgtaagc tacacgtttg ttgtaaacag cgataccgtt
     2041 gtacattggg ttttggttaa gctcttggaa agcatccatg ttcaattcgt taactggacg
     2101 tgatccaagg atcgctagga atggagtgtt atccatagct gcatcgtaaa caccgttaat
     2161 caagtgagtc gcacctggac cacctgaacc aactgcaacc ccgattgagc cgccgaattt
     2221 agcttgcata accgctgcaa gagcacctgt ctcttcgtgg cgaacttgta agaagcggat
     2281 atctttgtct tcagccaaag cgtccatcaa tgagctgagt gttcctgatg ggataccgta
     2341 gattgtatct acgccccatg ttttcaatac gttaagcatt gctgcagatg cagtaatttt
     2401 cccttgagtc ataatgataa ctctccttca atttttttag atttggagaa tacgattaca
     2461 tagaattgga aacgttctcc aaatttttac tattccactg tatcatattt atgctgactt
     2521 ttctaaaaat ctgctcaaaa ctctctattc tctattctaa tacagttttg aaagttctgt
     2581 catttctgtt ttataacaaa gaaatctagt cattactttt agtctatttt actaaaattt
     2641 aacagaaggg aactggtcag aacagataca gaactaaagg ccatggctag acctgccaat
     2701 tctgggttga gagccagtcc aacacctgaa aagactcctg ctgcaatcgg aattccgaca
     2761 acattgtaga taaaagccca gaaaagattg agtagaattc gatgaaaggt tttcttactc
     2821 atatcaaagg cacgaaccac tcctaaaaga ttattggttg tcaacaccaa atctgctgac
     2881 tcgatggcga tatctgttcc agctcccata gcaatcccca catctgctac actaagggca
     2941 ggagcgtcat tgataccgtc cccaacaaag gctactttcc ctgactgttg cagtttatgg
     3001 atttcatggg ctttttcttc tggcaagacg cctgcaatga cctcttcaat tccgatttga
     3061 tctgcaatag cacgcgccac accagcattg tctcctgtca gcatgactgt tcggagacca
     3121 cgttttttta gctgactgat ggctagctta gcattttcct taggaatatc ttgcaaagca
     3181 agcaagcctt tgatttcatt gtcaacagct aagaacacaa ctgtcttagc ttctttttct
     3241 agttcttcta gtttatcttg ataagtatta gaaatatcca taccatccag cattttagca
     3301 tttccaagta aaacttgttt tccattgatt cgccctgaaa cacctttccc gtgcaaggac
     3361 tgaaaatttt caacagtttg aaactcaagt ccagcttcac tcgctcgctt aacgatagcc
     3421 tcagccagtg ggtgttgaga agcatcttcc aaggaggctg ccaatccaaa cacttctact
     3481 tcgtcgccga tgatatctgt taccacaggt ttcccttccg tcaaagtccc ggtcttatca
     3541 aagacaagag tttgaacttt ctggatttcc tgtaagacag ttccattttt gaggagaacc
     3601 cccatcttgg cactacgtcc tgtccccacc ataagggctg tcggtgttgc aagtcccaag
     3661 gcacaaggac aggcgataat caaaaccgcc actccgtaga gaagagagga cacaaagcta
     3721 gctccaagca cgaccacact atccctgagc aagacgaacc aaacccaaaa ggtcatgatt
     3781 cctaaaatga caactactgg gacaaaaatc cctgaaatct tatccgtcaa gtcctgaatc
     3841 ggcgcacgac ttgtctgagc tttcttcaca aaatccacaa tctgagccaa aacagtctct
     3901 gagccaactt tttctgctct aaagacaagc gttccactat gattgatggt tgagccaatg
     3961 acagtatctc caactgtctt gtccacaggc agactctcac ctgtcaccat ggattcgtca
     4021 atactagaga caccttctac tacgacacca tcaacagcaa tcttttcacc gggacgcact
     4081 cgaatcaggt cgcctacctt gacttgttcc aaaggaactt ggacataact atcatcactc
     4141 aagacttctg cggttttagc ttgcaagtcc agtaatttct ccacagcttg ggacgtattt
     4201 tttctcattt tttcctcaaa aactgctccc aaaagaacga aaaagaggat aaatccagca
     4261 ctttcgaagt aaacagggag accagcaaag agagcaacta ggctatagaa ataagccact
     4321 agagttccca gcgcaaccaa ggtatccatg ttggcattgt gctttttaaa actggcccaa
     4381 gcactctgga tatatggctt acctgcaact aacataatag gcgttgttgc tagaaaggtt
     4441 ccccaatgca tgacttgatg actaatgcta cctgtcaaca tcccaatcat gagaatcaca
     4501 agaggcacag taaagatact agtaatccaa aaacgttgca ggagagatag agattttcga
     4561 gtcttctcaa cgactatata gcttcccttt tgcatcttca tgccacaaga aaattcatgt
     4621 cgccctaatt cttgaggtgt aaaacgaatg actttctcct catctacgcc gattggttcc
     4681 aagatacctt cttcttcaaa cagaatttcc ttataacagt ttgaaggagt agcacgatga
     4741 aaggtaatct cagctggaat tcccttttga agctggatat gggctggatg atagcctttt
     4801 tcagctcgga tacggatttt ttgaatgcca ttttctaagc ttgctttcac aatttctgtc
     4861 atagtctcca cctactctac aatcatcttg ccgtgcatca tgttcatacc acaagcaaag
     4921 ccaaactctc caacctgctc aggcgtgatt tccactacat actcttcccc cattggcagg
     4981 ttcgcatgta caccaaaatc tggaaaaaca atttgatcca gacatggtga aggatccttg
     5041 cggtcaaaga caatgcgtgc tggcactgat ttcttgagga caatcaactc aggagtatag
     5101 cctcccatga cttccactcg aatctcttgg tatccgtttt tttgctgggc tttttgtcca
     5161 gatttttcag gctttttgaa aaaccaaaac aagataaacg cgataagggc aatacaaata
     5221 atggttacaa tactatttaa catgacgtct cctttacata caattacatc ttacttctgt
     5281 tacagcactt gatttcttct ctgaaatcac agcttccaag tcttccaagt cagtctgagt
     5341 aaattcacat tctacaatca agtcagccaa caaattccta atcctacggg aacaaacctt
     5401 gtctttgata tcttggacaa gtaaatcccg actttggtct agagttaaaa gggctgaata
     5461 aacaaaggac ttgccttctt ttttccgagt cagacactct ttctcaacca aacgagccaa
     5521 aagagtttga atggtcgact tggaccagtc gaaccgctct gccaaaaccc taatcaaatc
     5581 tgtactggtc tgctccccct gcatccaaat aatcttcatg acctgccatt ctgcatctga
     5641 aatctgcatt accatacctc caaaatctac atttgtcaat tacactcatc agtatactct
     5701 taaaatctac atttgtcaat tatagaaata atattttctt cgaaaaatag aattttaatc
     5761 atttgaaaaa cgatttgcag tcaaatatta ctatataaac aataaaaata tgctatacta
     5821 aagaaaaaag aaaacaacca ctaggggtgc gtaaagctga gattaacgac tgttagatcc
     5881 ctctgactca atctaggtaa tgctagctga tggaagtgga aatgataatg gggactagca
     5941 gtcttctatt gcctttctaa aacagactag cttgttctta agaatacaaa cttcagttgg
     6001 ttgggaggtt ttagatgact tatttacccg ttgctttgac cattgcaggg actgacccta
     6061 gtggtggtgc tggcattatg gcagatttaa agtcattcca agcgagagat gtctatggaa
     6121 tggctgttgt aaccagtctt gtcgctcaaa ataccagagg tgttcagcta atcgagcacg
     6181 tttctcctca aatgttgaaa gcccaattgg agagtgtctt ttctgatatt ccacctcagg
     6241 ctgtaaaaac tggaatgttg gctactactg aaatcatgga aatcatccaa ccctatctta
     6301 aaaaactgga ttgtccctat gtccttgatc ctgttatggt tgctacaagt ggagatgcct
     6361 tgattgactc aagtgctaga gactatctca aaacaaactt actacctcta gcaactatta
     6421 ttacgccaaa tcttcctgaa gcagaagaga ttgttggttt ttcaatccat gaccccgaag
     6481 gcatgcagcg tgctggtcgc ctgattttaa aagaatttgg tcctcagtct gtggttatca
     6541 aaggcggaca tctcaaaggt ggtgctaaag atttcctctt taccaagaat gaacaatttg
     6601 tctgggaaag cccacgaatt caaacctgtc acacccatgg tactggatgt acctttgctg
     6661 cagtgattac tgctgaacta gccaagggca agagtcttta ccaggcagtt gataaggcca
     6721 aggcctttat cacaaaagct attcaagatg cccctcaact cggtcatggt tctggtccag
     6781 tcaaccatac aacttttaaa gattaagaaa aaaactctct agttcccact ttaagggaat
     6841 tagagagttt ttatactctt cgaaaatctc ttcaaattac gtcagcttcc atctgcaacc
     6901 tcaaaacact gttttgagct gacttcgtca gtcttatcta aaacctcaag gcagtacttt
     6961 gagcaacctg cgactagctt tctagtttac tctttgattt tcattgagta ttaattagga
     7021 aagaatgtca tgcaactttg ttaaaaaggc ttgcgttttt gcctcaatat cttctgcttg
     7081 catcaaatca cgtacaacag ctacaccagc tatgccagtg cccataagct gatcaatatt
     7141 ctccgaagtc aagcctccaa tagcaactac tggaatggca accgtttggc aaattgtttt
     7201 caaggtcgat atcagagtaa tgggcgcatt ttccttggtg gtggttggga aaatggctcc
     7261 tgtacccaag taatctgcac ctgatttctc cgcttccaga gctcttttaa ccgttttagc
     7321 ggtgacaccg aggatttttt caggacccaa gactttgcga gctaccgaaa ctggtaattc
     7381 atcatctccg atatgcagac ctgctgcatc aaccgcaaga caaacatcca accgatcatc
     7441 gattatcaag gggacctgat aggcatctgt tatttccttg acttgttttg ccagttgata
     7501 atattgattg gttgtgagat ttttttctcg caattggact atggtaaccc ctgaacggca
     7561 ggccgtctca acttttgcaa gaaagctttc cacggaatct tgatagcgat tggttaccag
     7621 atatagtcta agtgcttctc tattcataaa cctctccttt gatggtatct agccaatttt
     7681 catctcttct taggagcgaa agctgattga gtacttggta acgaaattct tccaatccca
     7741 ttccttgaac aactattttc tcagcagcga tattgagata agagactgct aagcaagaag
     7801 cttcaaaacc agtctttcct tggctgagaa aaacggctgt taaggctcca accaagtctc
     7861 ctgtccctgt tatccagtct aattcagtac agccattctc cagtacagca acttgattct
     7921 ccgaaacaat aaggtccttg gggccagtaa ctaagaaaga cataccagga taggtctgac
     7981 accagtcttt caagacttga agcaaatcct ccgtttcttg atctttagca ctcgcatcga
     8041 ccccaacgcc gtggtgcttt aatccaacaa gacttcgaat ttctgacatg tttcctttaa
     8101 ggaccgtagg tctatagtct aaaaggtctt taactaagct cttacgaatg gatgaagtcg
     8161 ttacgccaac cgcatctact accatcggga gagaagattg gtttgcatac gaagctgcca
     8221 tgcggattgc tttttccttc tcagctgaca aatgccccaa attgatgaag agagcctgac
     8281 tttgcttagt aaaatcaaga acttcacggg gatcatctgc catgacaggt ttgcatccca
     8341 gagccaaaat cccatttgcc agcatctcac aagaaatctc attggtcata cagtgaatga
     8401 gggaactaga gcctatagga aaaggatttg tcaatgcctg catcagtcta tcctttcagc
     8461 aaagaaatat ccttgcactt ttttaaagaa ttcctgcttg attaaaaatc taaatgcaat
     8521 aaaggaaatc gctgtaccaa tcaaggttgc tccgaaaaat cgaggagtgt agataaacca
     8581 actaagctta gcagccgatc ctgtaaagag caccataaca ggataggaaa caatagaacc
     8641 aataatacct gttcccacaa tttctcccaa ggcagaaaag taaaattttc gaccgtactt
     8701 ataaaagaga cctgctagaa gggctccaaa agtcgctcct gtgagagcta aaggcggaat
     8761 cccttgagtc gtcatacgga taaaggctgt cactgtagcc atagccaagg cataaacagg
     8821 tcccatcatg attcccgcta gaatattgac tacactggac atcggtgcca ttccctcaat
     8881 ccgaaagata ggtgtaagga ctacatcaag ggcaatcatc atagataaaa tggtcaattt
     8941 gtgaacttgt agttggtgct ttctcatgtt tctattcttc tcctttttct aaagactgta
     9001 aatcgctctt ccatgtctgg tgttggtaag ccatctccca aaacttggct tccatatgaa
     9061 cactgatgtg gaaggcatct agcatttttt gcttatctgt ctcatcactt tctcgataaa
     9121 gctgattgac cagtgctccc tcctctctga tctgttgctc taactcatcc gtaatataag
     9181 tttcaatcca ttgttgatag agaggatttg gtgatggttt aagattaagt gatttgccta
     9241 tatcatggta taaccaagga caaggaagca agcttgcaaa agctatggct aagttcggtt
     9301 ctgcaaattg cctataaata tgagaaatgt aatgataaca ggttggagcg attggatgtt
     9361 gctccatttc ctggtcgttg atttccaatt ccttgaaaaa ttgttggcga ataaataact
     9421 caccctccac taaaccctga gcattttgtt tcaagagtct tttcatctct tggtttgaag
     9481 tcttatcagc caaaagatga tagatttctg agaaagcctt cagatagtag gcatcctgaa
     9541 tcaggtaata gcggaaaatg gcaggttcta aattcccctc ttgtaattgt aaaataaagg
     9601 gatgatgaaa ggaagcctgc caagctttct tggataattc catcgcaata tctgtaaatt
     9661 ccataataac tcctttataa aaatagactg gtttgaagca ataaaaagaa aagcaggtag
     9721 attaattttg tttttttagg aatataaaaa gtccgatagc tattcttcaa ctgtgcatgt
     9781 tcgtcatatc cgtaagcaga tagagctctc aggtaaagat ggcgccacct aaagactgtc
     9841 atcagaacct tactgtaaat caagggcgac caaaaatgta gtttttgacc acgtaatagg
     9901 caagcttctt tgagggactt gatttcttgc tgaatgagag gaaaagaatt gaataccaca
     9961 atcaaggcat aggaccaaga gcgtgatagc cccttttgag ccaagtacaa gagaagctct
    10021 tttagtgaaa tagaggaaac aaagacaagg ccgatacaaa ctgtcacaaa ggccctcgtt
    10081 ccaagcatga ctgcctgtga agcatctccg tgtaactgaa ctgcccagta gttggcaaaa
    10141 gatgggaaaa tggcaagtat gatcatccaa gctaacattt taaatcgacg gtaatagagc
    10201 ataaagagaa tacaaaatgc gactaccgaa agattcagag caatcgaagg aatgaaagat
    10261 gtttccaagg ataaaatcag caagaagaga ctgataatcg gtgtctgggt tgctactttg
    10321 accatactat ctcacctccc cttgggtatt gctactctga gatgtaagtg gtttggtaat
    10381 ggtcacttct ttcacatgcc gaagaccctg actagtcatc tcaatccaat aatcaaccac
    10441 agaaatcaaa gggtctaaac gatgactaat gagcagaaaa cttcttcctt gattcctctc
    10501 ctccacaatc cacttgcaaa aataatggca ggctctatca tccaaacctg caaaaggttc
    10561 atctagcaag atcacggaag ccttactggt caagatggtc aggagctgaa gaattttttg
    10621 ctgaccacca cttaattgat agggactctt atcgactgcc tgctccaaat caaaatatcg
    10681 taaagcttga aaaatccgct gatttctttc agaatcaggt ccatctaatt gaagctcctc
    10741 tcgcagactg actcggataa actgcttctc agcttcctga acaacaccag tcagatcacg
    10801 atacaaactc tttttctttt tcaggaccga acccttccaa gtaatgctcc ccttatactt
    10861 ttgaaattga agaatagacc gaaagagggt tgatttcccg acaccattgt cacccaggat
    10921 acaggaaatc ccttgataga atgtgaaatc agcaattgaa aagagggggc gattaccaag
    10981 ctcaccagtc acacggttca tatggaatag ttccgggcta gaagcaactt cctttgaagc
    11041 aacctgtgtc atctcatagg aagggatttg aaacacttcc cttagttttc cgtctcttag
    11101 ctccaccata tggtcgatat aggctttata gtcagataaa tcatggtcgc acaaaataac
    11161 tgtcttccca tcatagacca actcttttag aatctccaat atctcgattc tgctcttgcg
    11221 gtcaatggaa gcgaagggct catccaagag atagacccta ggattcatgg caaagaggac
    11281 agccagcgct gctttttgct tttccccacc tgataagtga tggatgagac ggtgcaagat
    11341 gtccttgcaa cgacattgct ggacaacctc tgctatttta gaatcaattt cctgaaggtg
    11401 atagccgata ttttccatgg taaaaaccaa ctcctcaaac aagctctcca tggtaaattg
    11461 atgattagga ttttgcaaga gaataccaac cgtctggaca cgttcgacga tagaaagctg
    11521 actgacctcg ctcccatcta tcaggacttg accgctatag ggaagagaac taacttgggc
    11581 aatcatttga aagaggctgg attttccaga cccactactc ccaactaaca aggtaaaggc
    11641 ttgcgcatga aaagtaaaat caaacggctc agagaagatt ggggactgaa tcgctcgtag
    11701 ttccagaccc atctatgctt ttcctccagt tgcaaactga tgatagagtt tgacaatggc
    11761 acgaaccaag atggtacaga agaaataaac agaaataaaa cgtaccacaa gcaaggaaag
    11821 gacaaacgga agggaaaagg cgtagtaacc taacttaatg tattcataga caaagctaac
    11881 aagcgtaatc ccaatactat tagcagttag agagagccaa ctttcatagc gattcttagt
    11941 tacgataaaa ccaaattcac ttcccaaacc ttgaacaaag ccagacaaaa gagctcctag
    12001 accaaattgg ctaccataaa ggacttcagc aagcgcagct agcacttctc caatcgttgc
    12061 acttccgact ctcggaacaa agatggcagc aatgggcgca gccatacacc agagaccgaa
    12121 gaggatttca ttggcaaagg cctgcaaacc aagaggtgtt aagagtagac tgagaatatt
    12181 atacacatat cctgaaccaa cgaaaacccc accaaaaaag atagacaaga aagcaagcaa
    12241 gataacatct tttaactgcc attttttcaa cataaaaaac tccttttttt aaagaaaagt
    12301 ggggcactca agaagaccga cctaaatact ttgtatagca gactgaattt agaacagtac
    12361 acaagaacac taaaatattt ctagaaatta atttgaattt tctaattgat ttgttcgcat
    12421 cttatttcaa tctactatat catcttcatc cagtttcgta aaagaaaaaa ctctaattac
    12481 agatacaaat tagagttcag cttacaagat tagacagttc ttttcgacat acgaaaaaaa
    12541 catttcacat ttcccttcgc cagtcttaac tgtatcaggt tcaatgggta tcatctcagc
    12601 ctaaagcacc ccaaatgtct ttattattta attatgtgat tattataaca cacattttat
    12661 actagttcaa gaaattgaac tggaaataca gccttgcact cacaaagaca gcagatcttt
    12721 cttttgcaaa aaacaaatga cctgtttgat gaattagcca ttcaagctga atctggacat
    12781 agctttttaa aaaaggaaaa tcctacttac ttagaatcca aggatagata tctattgttc
    12841 actcatttcc cgaacagttt tttctatatt ttttgcatac gatattgccg aaatgattga
    12901 aacgccatcc atattggtct ttataatgtc tttaatatgt ttcgtctgta tcccaccaat
    12961 tgcaactaaa ggcatttgtg gcaatagttt tctcatcaat tcaagacctt cataacctat
    13021 agtaccacca gcatcatcct ttgactgggt accaaataca ggcccaacac ctacataatc
    13081 tacatattca acttttgatt gttgaaattc ttcttcgttt cttatagaaa gaccaattat
    13141 tttatctggc atcaattttc taatttcatc aacaccaata tcatcttgac ctacatgtac
    13201 gccatcggcg tcaatttcca ttgctaaatc tatatcgtca ttaacgataa atggaacatt
    13261 gtatttttta caaagttctt taatttggat agctagctca agtttttcta agccttctaa
    13321 agcaccctca cctttttctc gaaattgaaa taaggttata ccacctttta aggcttcctc
    13381 aacgactgta tatagatttt ttccttggca agtagtcgtt ccacaaataa aatatagttt
    13441 tagtaattct ttatgaaaca tcttacttca ctcttttgaa ttcctttaca tcttcatctg
    13501 taatctcgta taaggcattt ataaattcaa ctttaaatgt cccaggaaga tgtccatttg
    13561 gacgtttttc tgccatttct ccagcgatat tgtaaaccaa cattgctgtt tctaatgatt
    13621 tcaattcttg acctttttct agtccgataa agcttgctac tacagctcct aataagcatc
    13681 ctgtcccaat gactttcggc atcatagcac taccattatg aatcattacc acttctccat
    13741 taacagcaat ggcatccact tcacctgtta ctactattgg aatattgaac ttctcatttg
    13801 ctgctagagc aatttcgtca atattatcta cgcccgcact atctactcct ttagatgcca
    13861 catctattcc tactaaagag gcaatctcgc cagcatttcc tctaatcgct gctagtttat
    13921 aattgttgat tagatcatct gctacttttt ttctatattc tcctgctcca caggctacag
    13981 gatctaaaac tgctgggaca ttatatttct ctgcaatttt cagagcagct tggtataatt
    14041 tccaattttc atctgtcaat gttcctatgt ttattaataa accaccagca tactttaaca
    14101 aatcctctaa atctgctgga aactcactca tggctggtga ggcgcccagt gctactaatc
    14161 catttgctgt gaaatttttt actacatcat tggttataca aatgaccaat ggtgcttttt
    14221 cttttaataa ttttaaactt gtcatattga aatccttcct tttcacttta tacgatctac
    14281 tgatttcgat ttatttctat ctttcgttaa gaattttttt catttacatt gaatgattta
    14341 tactcaatga aaatcaaaga gcaaactagg aggctaaccg caggttgctc aaaacactgt
    14401 tttgaggttg tggatagaac tgacgtggtt tgaagagatt ttcgaagagt cttacctcat
    14461 caaatttgta aatatcatga gccttctcta gacatcgtaa ccaatatcaa aaaaagctaa
    14521 ttctaaagcg actgcttgat tccagcgttg ctgaagttct gtcaaatctt ctcgattttt
    14581 atcgacacga ttgagttcgt caaccagaaa ttgaacccac tctgcaaaga aaggacctct
    14641 gtggagattg atccattccg aatgaatata gacttcaggt aaagccaaat ctttagaacc
    14701 ccagtctaaa tagagacctt ctgcaatgac cagcatgacc aaaagatggg catagtctga
    14761 tgaagccacc gccgaataca ttagatcctg aaaggctttt gttacagggt gcaaagtcac
    14821 ttctagatag tcattctctg ctacttttaa ctctttaaaa gccttttgga aataaccatc
    14881 ttcatctgct tcaagaaagc ctagttgctt ggcaaaacga agcttggatt caagtttatc
    14941 tgcgtgagct acgcaggcac ccagcatgga taagaaggca tcaaagaagt gataatcttg
    15001 aatcagatag tcctttaaga ccttattctc aattgtcccc gcaaaaagtt ccttaacaaa
    15061 acgatgattg attgcagcct gccaatcctt ctgactgctt tttaataatt ctccaacagt
    15121 caaacctggc tgaaatgcat agtcttgtgt ttccatattt acttctcctc tctttacttg
    15181 ttagtaatta ataaaacacc aagaaatatc aagcaaaatc gtaattccac ttgatccttt
    15241 taaagcacat cgagagcatt tgcagagagc taactaaaca agcctatcca gtttatataa
    15301 acaaaaaact ccaattacaa tcaagaatta gagttgactt acaagattag atcgttcatt
    15361 tcgccatacg aaaaaacgtt tcacatttcc cttcgccagt cttaactgta tcaggttcaa
    15421 tgggtattat ctcagcctaa agcaccccaa atgtctctat tatttaatta ctgaaccagt
    15481 atagcaaaaa atgaaagccc tagcaagata tttgaccgaa aaatatcttt atatataata
    15541 tattgaaact agaatagtac acctctactt ctaaaacatt gttagaaatc gatttgactg
    15601 tcctgattga tttgtcctat tcttatttca ttttactata gttttcgata gcaatttatt
    15661 cttccaatac acgaagaaaa acctccacat tcagtggagg caatctgttt tatcaataca
    15721 attttaagtc acgagggtca actgggaagg ttgggttgta tggattgtga cggagcttaa
    15781 agtgtttgac atcttcaatg gtctgagctc cagacaattg cataactgtc ttcaattccg
    15841 cattcaagtg ttcaaagact tgacgcacac cgacactacc accgagagcc aagccataaa
    15901 taacagggcg accaatagct accaagtctg ctcctgaagc caaggcttta aagacgtgtt
    15961 gaccacgacg aacaccagag tcaaagacaa tcggcacacg tctatcaact gcttctgcca
    16021 cttcttgaag cgagtcaaag gcagctggtc caccgtcgat ttgacgacca ccgtggttgg
    16081 ttacccagat accagaagct cctgcagcaa gtgaacgttc aacgtcctca cggcattgtg
    16141 gtcccttgac atacacagga agaccagagt attcagcgat aaattctaca tcgcgtggag
    16201 acaagcgttg tttagctgat ttgtaaacaa agtccattga tttaccagca ccttctggca
    16261 ggtattcttc aacaatcggc atgccaactg ggaagacaaa accattacgc ttatccactt
    16321 cacgattccc ccctacagta gcatctgccg tcaagacaat cgctttataa ccttcagcct
    16381 tcacacggtc catgatgtgg cggttgatac cgtcatcctt actaaagtaa aattggaacc
    16441 aatggggtgt cccttgaagg gcttcagaaa tctctggaag gtcaacagta gagtaagagc
    16501 tggttgtata aagagaacca aactcatgca caccacgcgc agtcgccact tccccctgtt
    16561 catttgccaa tttatgagcc gcaacaggcg ccataatgat tggtgaagat agtttttcac
    16621 ctgcaaattc aatctctgta cttggatttt caacatcgca aagtgtatga ggaacgatga
    16681 gtttgtggtt aaaggcgcgg atattctctc ttaaagtgaa agtatcttcc gccccactag
    16741 cgatatagcc aaatgctgct ttaggaataa cttgttgcgc cattggctcc aaatcatagg
    16801 tattgatgaa gtctacatga ccttctgcat tgcttgtttt gtatgacata aaatgccctc
    16861 cttgattaag taagcgtttg ctttttacta cacaactata ataactcttt tttcaatcat
    16921 tttcaagagt tttgcttaaa aaccatctta ttttttcata caatatacta gccctacttt
    16981 ccttaaactc taaactttag tctatattaa gtgaatcgtt gagatttttt gctgccaggc
    17041 atttgaacta cttttttatt tgcggattct atctactaat actataaaaa aacaagtgaa
    17101 taagacacca gaacatcctc taataggacc tatggactta tatcagtccc atatatactg
    17161 tttaagcaag atactatcct agttgacgct aacttcctat cccacttatt aattttttat
    17221 ttatagtgaa tctgaacaaa tcaattgggg aatccaagtc aatttctagt actttttgaa
    17281 cagtttcagc gtactatcgt aaaatgaaat aaaacatgca caaattgctt agggaattta
    17341 aaccaatttc taacaatgtc ttagaaatca aagtgtacta tttcaacttc aatatactac
    17401 acttcatttt ctccttaaat tcagtctctt tttgaatcaa aaaatcccct ggtaagagcc
    17461 agaggataag aatttatttc attgttggga agagcaatac atcacggata gttgttgtat
    17521 cagtgaggag catgcagaga cggtcgatac cgattcccaa accacctgtt ggtggcatac
    17581 cgtattcaag agcttcaatg tagtcatagt cgatgcctgt cgcttcatca tcaccaagtt
    17641 ctttggcttt agcttgggct tcaaaacggc taagttggtc gattgggtcg ttcaactcag
    17701 taaaggcatt accgtactcc ttagtcatga taaagagctc gaaacggtca gtaaagcgtt
    17761 ggtcttcagg atttttctta gcgagtggag atacagctac tggatgtcca tagacaaagg
    17821 ttggttggat taaagtttct tcaacaaact cttcaaagaa ggcattgatg atgtgaccaa
    17881 cctcagtgta gtgtttctca actggaactt tcttctcagc agcgatagtt ttagcttctt
    17941 ccaaagtcat gtcttgccag aaatcgacac cagtaatttc tctgatagca tccaccatat
    18001 gaacacgctt aaatggttcg ttaatcttga tttcagtacc ttggtagttg actgggccat
    18061 cacctttgac tgatttagca gcgtgttgga taatgccttc agtcaagtcc atgatgtctt
    18121 ggaagtctgc ataagcttgg taaacttcga tagaagtgaa ctcagggtta tgagtagcgt
    18181 ccattccttc gttacggaag atacggccaa tttcatagac acgttccata ccacccacga
    18241 taaggcgttt caagtgaagc tcagtcgcga tacgaagcac catgtcaatg ttttgggcat
    18301 tgtggtgggt gataaatgga cgggcagcag caccaccggc ttcattatga agaacaggtg
    18361 tttccacttc aaggaatcct ttttggtcaa ggtaacgacg gatttcagag atgatttttg
    18421 aacgagtgac aaagcgttca aagctttcac gattagaaat caagtcaagg taacgtttac
    18481 ggtaaattgt ttcaacgtct gtcaaaccat ggaatttctc aggaagagga cgaagagcct
    18541 tagacaagtg tgtgatgtgg gttgccttga tagagagttc tcccatatcc gtacgcatca
    18601 cttcaccttc gacaccaagg aagtcaccaa ggtctgcttt tttgaagatt tcgtagtttt
    18661 cttcaccgac agcatcctta cgaacgtaga tctgaatctg gccttcgcgg tcttgaaggt
    18721 gggcaaaacc aacttttcct ttaccacgtt tggttatcaa gcgtcctgcg atagtagctg
    18781 tttcgttttt atcgtgtaat tgttctttat cgaggttggc atatttatct tttaattctt
    18841 gtgaatttgc agtacgttca aaacgttttc cgaaaggatc gattccttgt tcgcggagcg
    18901 cagccatttt ttcacggcga acgatctgct ggtcatttag ttcttccata tgttctgtag
    18961 acatgggatc ctcctagttt tactcaaaat tgaatacgat gagtcatttt ttgcgatact
    19021 cccattttat cataaataag caagaaattc acggataatc tttaaaaact aaaaagaacc
    19081 tgacgctaaa atcactggaa agaagtgaaa gtatctcccg ccccactagc gatatagcca
    19141 aatgctgctt taggaataac ttgttgcgcc attggctcca aatcataggt attgatgaaa
    19201 tctacatgac cttctgcatt gcttgttttg tatgacataa aatgtcctcc ttaataagta
    19261 agcgtttact ttgtgtatta caaaaatatc ttaactcttt ttcaaaactt ttaaaatatt
    19321 ttgtttggaa atttcagaaa ttttatgtct atgataaaaa tccttataac ggcaataaaa
    19381 aatagatatt atccaaagaa gattttaagt gctacaataa ctgtattatt tctagatggg
    19441 aggttctatt tttggattga tccattgttg aacaatatct accactatat caaaaggcat
    19501 tctttctgac cttgcatatt gcagtttggg gaattttggg atcctttctg ctcggtttaa
    19561 tcgttagtat catccgacat tatcgaatcc ttgttttggc gcaagtagcg acagcctaca
    19621 ttgaattgtc acgtaatacg ccccttttga ttcaactctt ctttctctac ttcggtcttc
    19681 cccgaatcgg gattgtccta tcttcagaag tctgtgcaac gcttgggctt gtctttttag
    19741 gaggctccta tatggcagaa tctttccgaa gtgggctgga agccatcagt caaacccagc
    19801 aggagattgg cctcgctatt ggtctgacac ctctacaggt cttttactat gtggttcttc
    19861 cgcaagcaac agcggtggca ctcccctcct ttagtgccaa tgtcattttc cttatcaagg
    19921 aaacctctgt tttctcagca gtggctttgg ccgacctcat gtacgtcgcc aaggatttga
    19981 ttggtctcta ctatgagaca gacattgcgc tagctatgtt ggtagttgct tatctgatca
    20041 tgctgctacc catctcactg gtctttagct ggatagaaag gaggctccgc catgcaggat
    20101 tcgggaatcc aagtactctt tcaaggaaat aatctcctga gaatcttaca gggattgggc
    20161 gttacgattg agatatccat cctgtctgtc ctcttatcca tgatgttcgg aacagtcatg
    20221 ggaatcatca tgacctccca ttctagaatc atacgatttt taacacgatt gtatctggac
    20281 tttacccgta tcatgcccca gctggtgcta ctcttcatcg tttactttgg cttagctcga
    20341 aactttaata tcaatatctc aggtgagact tcagctatta tcgtttttac cctctgggga
    20401 gcagctgaaa tgggagactt ggtacgtgga gctatcactt ctctccctaa acatcagttt
    20461 gaaagtggac aggcactcgg cttgactaat gttcaacttt actaccacat catcatccca
    20521 caagtcttaa gaagactgct accgcaggct atcaatcttg tcactcggat gattaaaacc
    20581 acttcattag ttgttttgat tggggttgtg gaagtgacca aagttggaca acaaatcatc
    20641 gatagcaatc gcctgaccat cccaactgct tcattttgga tttatggaac cattctagtc
    20701 ttatatttcg cagtttgcta ccctatttcc aaactatcca ctcacttaga aaaacattgg
    20761 agaaactaaa tgtctgaaac tatcttagaa atcaaggaac taaaaaaatc cttcggagac
    20821 aatcccatcc tccaaggact ttctctagaa atcaaaaaag ggaagttgtt gtcatcctag
    20881 ggccatctgg ttgtgggaaa agtaccctcc ttcgttgcct caacggctta gaaagtattc
    20941 aaggtggaga tattcttctg gatggtcagt ctatcgttga aaataaaaaa gattttcacc
    21001 tagttcgcca aaagattggc atggtctttc aaagttatga actctttccc catctggatg
    21061 tcttacaaaa cctcatccta ggccctatca aagctcaagg aagggacaag aaagaagtaa
    21121 cggaagaagc tttgcaatta ctagagcgtg tcggtttgct ggataaacaa catagctttg
    21181 cccgtcaatt atctggtgga cagaagcaac gtgttgcaat tgtccgtgcc ctcctaatgc
    21241 atccagaaat catccttttt gacgaggtga ctgcttcgct ggatccagaa atggtgcgtg
    21301 aggtgctgga acttatcaat gatttggccc aagaaggccg taccatgatt ttagtaaccc
    21361 acgaaatgca gtttgcccaa gccattgctg accggattat cttcctcgac caagggaaaa
    21421 tcgctgaaga aggaacagct caagccttct ttaccaatcc gcaaaccaaa cgagcccagg
    21481 aatttttaaa cgtctttgac tttagccaat tcggctcata tctataaagg agattcttat
    21541 gaaactattc aaaccactct taactgtttt agcacttgcc tttgccctta tctttatcac
    21601 tgcttgtagc tcaggtggaa acgctggttc atcctctgga aaaacaactg ccaaagctcg
    21661 cactatcgat gaaatcaaaa aaaaagcggt gaactgcgaa tcgccgtgtt tggagataaa
    21721 aaaccgtttg gctacgttga caatgatggt tcttaccaag gctacgatat tgaactaggg
    21781 aaccaactag ctcaagacct tggtgtcaag gttaaataca tttcagtcga tgctgccaac
    21841 cgtgcggaat acttgatttc aaacaaggta gatattactc ttgctaactt tacagtaact
    21901 gacgaacgta agaaacaagt tgattttgcc cttccatata tgaaagtttc tctgggtgtc
    21961 gtatcaccta agactggtct cattacagac gtcaaacaac ttgaaggtaa aaccttaatt
    22021 gtcacaaaag gaacgactgc tgagacttat tttgaaaaga atcatccaga aatcaaactc
    22081 caaaaatacg accaatacag tgactcttac caagctcttc ttgacggacg tggagatgcc
    22141 ttttcaactg acaatacgga agttctagct tgggcgcttg aaaataaagg atttgaagta
    22201 ggaattactt ccctcggtga tcccgatacc attgcggcag cagttcaaaa aggcaaccaa
    22261 gaattgctag acttcatcaa taaagatatt gaaaaattag caaggaaaac ttcttccaca
    22321 aggcctatga aaaaacactt cacccaacct acggtgacgc tgctaaagca gatgacctgg
    22381 ttgttgaagg tggtcattaa ctcttaaaag ggactggatt ttaagctcca atcccttttt
    22441 aagattttac ctataacatc ctgagtctat ctaagatgtt caatctgaac acagtgtaca
    22501 tactttatct tctattgcat atactttatc acataagata cgaatatcct cttcactatg
    22561 actagcaatc aaaattgttg tccctttttc actagagagc tttctaaaca atgttctcat
    22621 attttctaca cttgatttat ccaaggcatt cataggttca tctagtaaaa gaatagaggg
    22681 attctccatg attgcttgag caatccctag ctttttcctc atacctagcg aataagtttt
    22741 aactttctgg tctttttgct catatagacc aactattttc agtgtatcat tgatttcctg
    22801 attaccaact actcctcgta tgcttgccaa atattgtaaa ttcttaaagc cactataata
    22861 atttataaaa ccaggttctt caatcaaagc tcccaaatta gctggaattt ttctctcagg
    22921 aacaatattt tccccattga ttaacacttc tccataagac ggactatata aaccagctat
    22981 taatttaaac aatacacttt tccctgagcc attcgcacca gtaattccta taatttcccc
    23041 ctgtttacaa ctaaagttaa ggttttgaaa aacacatgtc ttttttaatt tcaactcaat
    23101 attttttaat ataattattt cattcattct ataaacctcc tcttttgacg agtgaaatag
    23161 aaaatgcttt gaaaaagaaa gactaaaaat agcaactgaa gaaacaaatc tccccctaca
    23221 tccccattcc ctcgattcaa aatataaaat agataattag ttcgatttcc tacaaataga
    23281 ccaccaaaca caatcatgag taaaaagaaa ctaacgcaag caaagttcat ttgtacattc
    23341 gctcctagtg cgtataacat aaagcaaaac aagactaaca cacaagcatt caaagaatca
    23401 actataaaca aaactcctat atcccagttt ggtaactgta acataaattg aatcactaga
    23461 acttttacca tgattaaaat gagtccataa agaaaacagg tccaaatctg atacctaatc
    23521 cagagcttaa tagaagaaaa tcgaatcact tccataaaga aatcggatgc tcttctacga
    23581 tatacaactg ctagaaaaaa tgtatgaata ataaaccaat gaagcaacca tatagataag
    23641 actaaaaaag agttttgact cagattaggc ccttttaaaa tttctataac aagtctatca
    23701 tttccttcta accaatacca taatcctaaa caaatcaata tccaaaaaag aacataaccc
    23761 caaaagtact tggtatagat tatcttattc aacctccaca tgtgactact taactttccc
    23821 atttccaact tttacactct cctccagttt ctatgaacag aaaatacagt caatggaatt
    23881 atgatagata gtaatgtaag ccaataaatc aatgcatacg gattggaatt taccatatac
    23941 tgaaaaattg gcaataatcc aattatccca ttgttcaact gataaatcca gaataaggat
    24001 tctacaaata aaaaagtaaa cagaaatact aagctcatgt ttgatttttt aaaccaagaa
    24061 aaactgctaa tagtaagtaa ggataaaaag aaatagtatg ctatataaga gaaaaaaaat
    24121 cctataaaga aactagcatt gtttgcaata cttataccat aaaattctct taaaaaatca
    24181 acctccttta tctccaaaga gaagctaaaa ccattactaa atgcaattag aaaaatcaat
    24241 aaaaataaag tcgccgtcca aatccccgta ctaagagctg ctaatttgaa actaaaactg
    24301 gtaaagtgct taattgattt cagacgaatg cgacactcca acctattaaa atagttattc
    24361 atcaaataaa aaaagaacaa tatatatgtg aacggaaagc aatatactcc agtcgtcata
    24421 tcttgaagta aaactaaaat ccattctaat acatttggat ggattgaata ttggcgacag
    24481 cgcaataaat atactgtact agataaaaca caggatagca gtaatataaa ataaaccaat
    24541 actgataaaa aatctttttt gtaaaattga acaaattgtt tcattataca tagtcctctg
    24601 aatgtagaaa aaatgtacca taaacaacca aacaactaac aaataaaata aaagcaagat
    24661 gcccactaac taaggaaaga ctgatatctt tctgatatcc caaagctaat gttgtcacag
    24721 gttctaagta agatagccct aaaatagccc aaaaaatacc accaaccatc atataggcaa
    24781 ctgggatgaa aatagctcct atttttttct tcactagcaa agcactagct agtccaaaaa
    24841 tagagaacac agcgccccaa actccatacc agagagtcgt cacaagacta tagagcaact
    24901 gattagaatc aaataattct tttaaggcac cactataatc tccaatataa atttcctgat
    24961 aaggagtcac taaaagatta attcctaata ataataaata ggggagaaaa aagactagaa
    25021 aagaagaaat aattgcagta ctacctacaa tagccagata cttcttttta gaaattcggc
    25081 acaattgtgc tgttagaaaa tgactctcag catcctctat tatctgacta gaatagggca
    25141 gtgtacagat aagtgcagct actaggctaa tcggtgaaaa tcctcgaata gaagaagcgg
    25201 caaaaaaaat cgataaacct tcaattttat aaataccatt gaaagcaagg aaatttccta
    25261 aactcatgca aagaagggtc aaaaataaag acatataaaa tcgaggtgat tgaacgactc
    25321 gtacaagatt acaaatgaaa aattccatcc ttactcctcc ttataataaa aatagggtgt
    25381 agcattcttt tttcatgcta cacccacaat caaccatctt taaggcttac tctgacaagt
    25441 aagttaataa gaatctggac tccaagaacc tgaagtatga attcttacat gatttccaaa
    25501 ttgtggcgcc atagctaatc tagtaccaga accaatataa ttgtcaccac ctccattata
    25561 gtacatgaca atcctagagc cagaccccaa tgaatatacc ggggtaatat ctgacccact
    25621 ataggcgcta cgaatagagg tatttaacct tttaccgcca ccagtgctgt cactattatt
    25681 aattccagca gaggcgtttt ctttctcaag ctcactagtt gtatccgaac cccaacgcgg
    25741 caacgttaaa tcataatttc caatctgtct agctaaaact gtagggagaa acaaactaga
    25801 tgcaattgta actacgcaaa aaaggaatat atttttcttc ttttcatgac atcgtcctct
    25861 taagaagtga taaacaagat agttgattga tcaatttgat acatattaca ttttcattat
    25921 atcaataaaa gaaagcgttg tcaattattt tatttaaaat attgtcatat tttaaataaa
    25981 atggtaaaag agactcggta gcctcgtctc tctttatctt actgacctaa ccttcttgcc
    26041 aattttcttg gtcttcttta aagcgtttta gaagatcaag gccctctggc gtgatgtaac
    26101 cttcttcttg ggctagatgg ataagctctc tatagtttga aagcgtcaca agtttaacac
    26161 cagcatctgc aaagttctta tctgcttttg gcaattggta gctgaaaatc gctacaactc
    26221 caagtacatc tgctccttct cgcttggctg ctgctacagc ttcaagaact gaaccacccg
    26281 ttgaaataag gtcttcaacc actaccattt tttgaccttg agctacgcga ccttcgattt
    26341 gattaccagc tccgtggtct tttggtttgc tacggatgta ggcaaaaggc aagtccatct
    26401 tatcagcaat aatggctccg tgtggaatcc ctgctgttgc agttcctgca atcacttcta
    26461 cttcaggaaa ggcttcttta atagcttcca caaaaccatt ttcaattagg gtacgagttt
    26521 ctggataggc tagtgtcaca cgattatcag tgtaaatcgg tgacttgata ccagatgccc
    26581 aagtgaaggg ttcctctggt ttgaggtaaa cggcttggat tttcaagagg tggctagcga
    26641 tatctttagc aagtgtcatg gtattctcct tttatttttc taatctagtt ctttaattcc
    26701 agttctgtgt ccattcatcc ttgatggcat gataagctgc aacaggatcc tcagcttggg
    26761 taatgggacg tcccactacg atatagtcac tgccgatttg ataggcatca gcaggtgtca
    26821 tgactcgttt ttgatctcca actgcaacac cagctggacg aatccctggt gtcagacaga
    26881 taaaatctgg attggtagcc tgcttgatga cttgtacttc ctgagccgag caaacaacac
    26941 catccaagcc agcttcagct gtcttcttgg catagtgaat cacagactct tgcagactgg
    27001 tttggatatt ttgaaactcc tgcatctgag cttctgacgt tgatgtgagc tgagttacag
    27061 cgatcaattt ggcttgactc ccaagacctt cacgcgccgc cttcatcatc tctacaccac
    27121 cagccgcatg gacattagtc atatcgacac caagctgaga caagaccttc atggctgact
    27181 tgactgtatt aggaatgtca tgaagtttga gatccaaaaa gacactatga cccaaacctt
    27241 ttaagtagga cacaatctca ggccccgctg cgtaataaag ctccatccct accttgagat
    27301 aaaggctttc ttctgctggg aaaagagcta aaaattcctt gaccgcctca aaactaggaa
    27361 aatcaagagc aatgattgga cgatgttctc gcataggttt ctccttttac ctttactagt
    27421 gagagagtct ggccaacaga agccacgaaa aaaggaacct gccaacagca aggttccacg
    27481 aaaaatgggt agtctccacc ctttcaccgt ctaaccttag ctgcctctct ggactgcttt
    27541 aaaggttttc tattattcta ttatttctac tagcacttgt caagtaaaaa cttagtcacg
    27601 tatgtgttga ctgctattga acacctcaca agactctttc caaaagaata gttaaagact
    27661 ttcacttagg acagagaaaa agagtttgct tgttaccctt taattgaaca aatgggaatt
    27721 ctgttttatt ttgcggatgc ctattcatct tggcaaaggg gaagtaatga gaatgcgaat
    27781 ggacttcttc gtgagtattt tcttaaaccg atttggcaac aattaccgaa aaaatcctta
    27841 atggtgcttt gctagcttat taatcaccgt ccccaaaaat acttggatta caagacaggt
    27901 tttgaagcac ttatagagga aatttaaaaa tgttgcattt tttattgcaa tttatcagtt
    27961 atcttatcac ccaaaacttc tgcaattagt atgagcacta ttgttatttt tcttttttcg
    28021 tacactcaaa tcaaaatata gcaataagac tattctgtag aattgctgtc aaaaagatca
    28081 gttcattttt ccttcccact tgtgtttata atcattaaaa taactcaatt aagaaatttt
    28141 ctttttccta atagttatcg taattaaagt taatagaaga acaatccaaa taagtagtgt
    28201 tagattagga attagagttt tattccatga aaatccctcc cattttgtga aaaacttagt
    28261 aaacagtccc ataaaactat aatctgttat cttcgcaact gtcttatcca aaccagatag
    28321 catcggtaag aaagcaacta aaatcatagc aggaagactg ataacctgag ctactacttg
    28381 gctcttcgcg gttaaaccta tcaataaata gaataaaatg atggataaag aggttagaag
    28441 aacgattgta atataattaa aagtatgtac aattgtaact cctaaaataa gaggagtagt
    28501 tcccataatc acaaaagtta gcaaaaaagg aagaaacatc gttgataaaa tgtattcgga
    28561 tcctttaaca ccactcaaca gaagagtttg taaattgtac ttttcttttt cttcagacaa
    28621 gataatagtt ataggacttc caacagccaa agccaaagaa aaaggtaaac acatcatcaa
    28681 aagaactaat gcctgttgat cgttgacctt cccctgtgtt tccataagat atttataaaa
    28741 atatgtgaaa gcaaaaggca ctaaaacttg caataaaata ctcttattgg aaaggataat
    28801 ttgacaacgc aaccatacca aagccagaaa ccgtttaagc atttagcttt cctcctgtta
    28861 atgtgataaa aatatcttct aaagtaggct cacatgaatg aattgaaaaa atattttcca
    28921 gatcagcctg agatacctgt tctagtgatg taaaatcaaa agttatctga ttcccattat
    28981 aatctgtaac cttaatcttt ttatctttat tatatctttg aatgagttca gaaggagctc
    29041 cttgctcaac taatttccct ttatttaata aggcaacata atcacataaa agagttgctt
    29101 cattcatatc atgagtcgtt agaaaaatcg ttgtccctgc tgttttcaat tctaaaatta
    29161 actcatgaat tgttcgagaa gttgtgggat ctagacctga ggtcggttca tccagaaaga
    29221 gtacagcggg tttgttgata agagctcgtg ctaaaagcat tcgttgcctc attccagtgg
    29281 ataattttcc tgctaccatc ttgcgactat catataatcc tactcgcttt aacaaattat
    29341 caacacgtga tttactaata ttataaaatt tactataaaa aagaagattg ttatacagag
    29401 acattttctc ataaaatcca cttgtatcgc taaccaatcc aattctcttt aattcaccgc
    29461 ttgttaaatt ttgagatttt tgtcccaaaa taatagattg tcctttatcg gcaaggaact
    29521 gcccagtcag aatattaatc gttgtggtct ttccagaacc agatggtcct aaaaatctaa
    29581 ccttctttaa cagtgaagct tatattattt aaagctattt tatcaccaaa actttttgat
    29641 aaatggctaa cttcaatcat actcattgca cctcctttat attctacatt tttacttaac
    29701 atctatttac ttatctttat caaataaacg tcttaaaaaa aacaataaca ttaagcatat
    29761 aaccgttagc aagattgttt caatattatt gataatacga tacattctca agcagtataa
    29821 aataattata tatatgcaaa tgctcattat acctattttt ttaaacatac taagcctcct
    29881 tgctaaataa taaagttata taataactaa gacacactat acctaacata ataaatatcc
    29941 actggtaact gaataaatgt tctaaaatta acaagcaaaa ataaaatctc aaacgatgaa
    30001 aaaagccata ctatattttc caatgatgtt tgaatatttt tattgtagta agcataatat
    30061 atagcaaaaa taatacccaa gaacaatact attaattgtg tttctataac agtgaactgc
    30121 ccaatagcag ataaaaaagc tactcctcct gataataagc aaaaacaaac tgaacgaata
    30181 aatgttttcg ctctaatatt gttatctttc actttattat ttttttacaa attggacatt
    30241 ctccgttttc cagaacatat ttttcttgac ttagagcatg atttctcata ttataaacgt
    30301 aagcgttatc tattaggatt tcattataac atcccagaaa tattttaatt attaaattag
    30361 tcattatccc tcccaaaata gcaggcatct ctggtgtagt cggccacttt gtcttatcag
    30421 ataaagtgta cttatttata tctttgttta tattccgaca accaaagcaa gaaccgatgg
    30481 tagggatata tattatcagt aataagatag catccattaa accctcctga tatgtaggat
    30541 atcttatgcg atacagcaaa ttgattgaca atttttataa tatcaatggg cgtatcaatt
    30601 gctttaacga taaaatctat actcccatat tccgcaacaa ttttttctaa ttcttctact
    30661 gaagaaactt taatagaaat aggtactacc tgaacatctg aattatactt gtgtattttt
    30721 tcagaaagaa cattaatctt ctccttacca acatccgact ctgtataaag aatttgccta
    30781 tttaaatttg atggttctat tatatcgtaa tcagcgacaa taagtttcct cactccatac
    30841 atacttagac ttagagatat ataacatcct aaggctcctg ctcctaatac taatactgtt
    30901 ttattctgta ttttattata ctcttcatta taaaataacg aatagtaacc tttagttcta
    30961 gaatatctac tatcaatatc tatctcatcc gaaattaaaa tttgttcatt taatagttca
    31021 aatgttagtt tactgcctaa tacttcttct atctctaata gttccttaca actattaaat
    31081 agatcgttga taaattcttg cgaaatttga ggttctatag ctatttcccg catactccga
    31141 ccacgcaaat acattatacc ttctttttta taagcaaaaa cataactatt tacgtagaac
    31201 atatttcgtc cttcctctca tatataatat agaaagcagt atagaaacaa ccacccctcc
    31261 tatactacga acaataatat taggtgtcaa aaactgtaat agttgactca aagggaaata
    31321 aataataaca aatgctatta aaagttgaac tatatattga aaattcaagt tattattgtt
    31381 agaattcaag tatcttaaaa taataccact atcatactga aaaaaaggaa ttgaattaat
    31441 aaaaatcata cctaagttaa tgataaaaaa taagagtcca agcaatttat acgatgtgaa
    31501 caataaaaca agtaaacctg taagtgataa aatcgaattt gaagcaattc ctgctaacat
    31561 aattttcaaa aattgttttt tattttttaa gaattgcacc ccacttaaat ctacaaaaaa
    31621 aagccaaatt aaacaaaagt agcattattc cacaatttgg gatctcaatt tgataattat
    31681 tagcaattat tgcatgacta aattcatgta tcgttgcaga cataataaca acaagcaacc
    31741 aaaataaaag aatgtttagc gttattggaa agttttggaa attagtcaaa atatccgccc
    31801 tataaatata cataataaaa cattgcaaaa taagagccaa tgggaaaata ccaaatagga
    31861 ttttttcaaa attcttatta aatcttttat agtttaattt ttcagataat gttatattaa
    31921 agtcccaaga tattttaaaa atatttatct tttttcgtga tttcatagaa tctataagtc
    31981 ccattttact aagaacaata taaaatctta aataatattt cttccctact aacactaata
    32041 agtccgatat ggtaaattct tgacctatta gttgctctaa ccattttttt cctgtttccc
    32101 aactaataca tgtttcttac tagatggtat atatagatga tactgtccct cataaggagt
    32161 aggtaatatc ttaatcttat caacaattct cataacaaac ctaaccttta ataaataaaa
    32221 gaggggctaa gtaaaacata ctctagtccc tcagtccgca atcaataaaa taatgactaa
    32281 ttctattaat ctcttgctgc aagactaata cggaaaattc aaatgactgt ctaaaatgaa
    32341 attgaaaaag cttgatgtta gtaaatgaac aaacttcaac aaccagcaag agcaaggatt
    32401 gctaaaccaa atccgatacc tgttgcaagt ccagctgaaa aatcactaac tggtactgaa
    32461 atttcttcaa tttcctcaaa taaaaagtta tcttgcatga ttatcactcc ctatcttttt
    32521 aacaacaggc tattccaatt aagagaacta ccccagttcc aagtaaaact cccccaaaaa
    32581 ttcaccatct gtaggggcta cgatttcttc aatttcttca aataagaatg cttcatctga
    32641 catatgtgtt accttcttca taaaaaatta ttttgtaatc gattacattg caaatgtaac
    32701 ataaagaaaa acagatgtca aatattaaac gtaagaacat ggtcactaaa gaactataag
    32761 agaaaaggta aacctagcga cgcgatgagc gctgggtcgt ttgatttcga ttgctctctt
    32821 cctcttgttt tttctgttct tcttcctgtt ttttctcggc ttccttggcc tcttgtttgg
    32881 ctttttcctc agcctccata attaatttat ccgccacagt gtaactgtaa attccagctt
    32941 ccatgtcgac cacactcggt tctgacaatt gtggcttaat cttactgtaa tatggcagtt
    33001 tcttactcat ttcagataga ggaaccaaga cttcgtccga atcattcatg gtcaatcgaa
    33061 ttaaatcgga tgtcaccttg cttggggcta attccacctt ttggatagcc gccttgagtt
    33121 ctgggctaat ttgagcaagt tctgagacaa aaaccttgat ttgttcacta tcattaaaga
    33181 gaactgataa ataagtttct ggtaaactgt tcagactcac agaactagtc tcaagctgac
    33241 cactggaaag aataggataa tgattttcac cagaaatata gtaggccaca atatcatatt
    33301 ccttgacctt aatagtgaac ttagttggaa attgatagac aagttgagct gattcaaccc
    33361 aatagttaga cttaatctgc ttttcatatt ttgccttgtc tagcagaagg ttaatcgtat
    33421 aatccgaatc ctgaatgcct gaagcctgtc gaatatcatc agctgtagtt tgcaccgttc
    33481 cctcaacacg aatatctttc atggtcgcat aaggactgag caagtaggca gagacaatca
    33541 ataaaagcag acttggaaat aaaatcgtga aggctcgcaa gatatggata ccaggaatct
    33601 ttgctttggc tggtttttcc tttgtagcct ttttagaaag ctttttatcc tgttcctcct
    33661 tctctttaga ctctggttct tctttctctt ctttctcttt gtcagcctct gaggatgcta
    33721 ctttttcttc agactcttcc ttagctgatt ctgaatcttc ctggtctgtt tcactctcct
    33781 ggtcctgttt gtcctctgac ttctcagatt cttctcccat tcgagcttgt ctttcctttt
    33841 ccttctcctc agctagagcc gcctcttctt cagccttctt ttttagatat tcttggtttc
    33901 gtttctgcca ttctgataac tctttcaatt cttcgaggat ttctttgtcc tcatttttct
    33961 tatcttttga catttacttt ccttatgata aatctttttt caacaattga taaaaatctg
    34021 ctagagattt caattcctta gaagccttca tcttagcttg gtaatcttcc ttgtgactta
    34081 gtaagtgaga aagcttctct tccaaactat ccaaggtcaa atcgctttct tgaaggtctt
    34141 ctgcatagcc tttttttaca aagtaagctg cattttcaag ctggtcacca cgactagctt
    34201 cacgaccaag cggcacaatg acatgcaatt ttgctatcgc caagagctca aaaatcgtat
    34261 tggcaccacc tcgtgtcaca acaatatcag ccaattccat caagggttga tagagatcgg
    34321 tcacatagtc aacacgaaaa agattttggc tcaactcatt caggctagaa tctccagtta
    34381 gattgataat attgtagcgc tctgttagtt ctttcttatg gtctgtcacc aattggttaa
    34441 agacacgagc acctgcagaa ccgccaacaa acaatacagt cggcaattta tgattaaagt
    34501 ggctttgaat atccaccaat tcatctggtt ctggattttt ttgatctgaa accttggcca
    34561 ctgctcccac atgctcaacc ttagacaaac tcgaagcttg ctcaaaggtt gaatacatct
    34621 tagtcgcaaa tttataggcg attttattgg ccaagcccat agacaggtca gattcgtgaa
    34681 taaagacagg cactcctgac acacgcgcag cgataacagg cggtactgag acaaagcccc
    34741 cctttgaaaa aagggtctgt ggacgaagtc gcaacatgat aaagagcgat tggacgattc
    34801 cccagcaaac tttgaagacg tccagcatat tttgccaaga gaaatagcga cgcaattttc
    34861 cagtcgcaat agaatggaag gtgacatcca aacctgactt aaggatttct tggtgttcga
    34921 taccacgctt gtccccgata tagtggactt cccaaccatc ttcgatgaac ttgggcatta
    34981 acaaaagatt gagggtaacg tgtccaaccg tccccccacc tgtaaagaca atttttttca
    35041 tattattctt ttaactccgc tactgtgtcg ataaagaggt cgccacgtac ttcaaagtta
    35101 gcatacatat cccagctggc attggcagga ctaagaagaa ccacatctcc ttgagtcgca
    35161 agctcatagg ccttgcgggt cgcatctgca atatctgtcg cctccacata agcgacacca
    35221 gccttgtctg ctgcccgttt gacacgttct gcagattgac ccaggatgac catcttcttg
    35281 agtccagtaa tgtctggcac caattcgtca aactcattgc cacggtccaa accacctgca
    35341 atcaagacga ccttgctgtt atcaaatcct gacaaggctt tttgagtagc caagatatta
    35401 gttgatttac tgtcgttata gaatttaaca cccttgatgt catccacaaa ctggagacgg
    35461 tgtttgacac caccgaaggc tgaaagagtt tccttgatgg tttgattgtc cacaccacga
    35521 agcttggcta cagcaatagt cgcaagggca ttttccacat tgtggctacc tggaacaccg
    35581 atttcattcg ctgccatgac tacttcacca cggaagtaga gttgaccatc ttccagataa
    35641 gctccatcaa ccttttcaag tgttgaaaat ggtacaacag tggcttctgt cttggaagtc
    35701 aagtcttttg ccaagtcttg attaaagttc aagacaagga aatcagctgc tgtcatcttg
    35761 ttctggatat tccacttggc tgctacatat tccgaaaatg acccatggta gtcgatatga
    35821 gttggcatga ggttggtaat aaccgcaatc tctggatgga attcttgaac acccatgagt
    35881 tggaaagaag aaagttccat aacaagcgtg tccttatctg atgctatttg agcaacctga
    35941 ctagctggat agccgatatt ccctgataaa agaccatgtt gcccagcagc agtcaaaact
    36001 tccccaatca tagtcgttgt ggttgtctta ccgttcgatc ctgtgatacc aataatcggt
    36061 gcttctgaaa tcaaataagc caattccacc tcagtcaaga ctggaattcc cttggccaaa
    36121 gccttttcaa tcatgggatt gttgtagggg atacctggat ttttcaccat aagggcaaac
    36181 tcttcatcca agagttccaa aggatggcca cctgtaatga ccttgatccc ttcttccagc
    36241 aaactttggg cagctggatt gtcctcgaaa ggtttcccat catttactgt cacaatggca
    36301 cctagcttgt ccaacaaacg agctgcagat tcaccagact tggccaaacc taaaacaagg
    36361 actttcttat ttttaaattg atctattact ttcatgtctc gaactccatt tctactccta
    36421 ctattttacc atttttatgg aaataaaaaa gccacaaagt gtgtttgtga ctctttcttc
    36481 taactgaatc ttaccatatc atctatgtga taaatcggta actcgaatga cctgatccac
    36541 ttgttcccaa ataagaggat tgtgggtcgc aataataatg gtccgattcg gattttttaa
    36601 agattctagg atggaaagta attcctcaga gtttttgggg tctaaggaag tggttggttc
    36661 atctgcgagg atcaaaggtg gatcctttaa aattatcttc gctaatgcaa cacgttgtgc
    36721 ttctcctcct gataactcaa atataggttg cttcaaatcc aaataagaga ggtttacaca
    36781 gtttagagct tgtttcatca aagagatttt ctctttttcc ttcaactttt taccaactaa
    36841 acccagattg agattctctt tgacggtttg gctttcaatt aagccaaaat cttgaaataa
    36901 gtatcccaag taatctctaa agaaaacaga aggcttgatg tccttaagag aagtaccatc
    36961 atagatgatt tgtcctttgt catatggctc taatcgtcca atcatattca agaatgttgt
    37021 cttaccacag ccacttgtac cgattaaggc ataaattttc ccaccttcaa aatgaagatt
    37081 tgtatctgaa aatagctgac ggcttccaaa ttttttagat atattcttta gttcaatcat
    37141 cctattttcc tttcataatt gtcatagaaa cacgagattc tttctgcgct tgacggtaaa
    37201 gcgtcagaac tgcactagct agaaagacta ataaagtgag caagccaatc accaagtctc
    37261 gactgcttaa aataaagaga ctagcaccaa atacaaaact ggcaaattga ctaaccatat
    37321 actgagcatg tgtttcaaaa aatcgtaaac ctgaaattcg tttaatcaaa atatctcggc
    37381 ggaattgctc gaaatataga agattgacag aataaaatag taataaggaa ctagctatcc
    37441 ccacaatagc tcctaagatt aaagttgctg tttcagtttg aacttcatta taacgagtta
    37501 gataaacact tcttccttct ttaagatagg atacttgctc ataaattcca gctttcttca
    37561 agagttctag cccactctca tatcctttga taaagagttg ttttccagca ttgatagacc
    37621 aactagataa ggatataaaa ctatcacctg tagaagtcgg cgtgaatacc actaaaatcg
    37681 gatcagtcaa atactgagta gatacgggat tctcaccgtt attataaaca aaccgctttt
    37741 ctcccattga aagataacta acgtgcgctt tcatctcata atccaaagga gcacttgcct
    37801 cctcaccaga ttttccataa taactcaatc tttcttcaaa aactttctta agttctgctt
    37861 ctcgagagcg caaatgttct gggagcaaga ggataaactc acctttttgg agatgggcta
    37921 acttctgttt ggtctcagca tctaccacga ccttttcctt gtccaaataa ctgggactaa
    37981 catagagcgt attagcatct ggactatagg tatccagtgt ctctccctgt tcattttttc
    38041 cttgtggatt ggcaaaatgg agcagattat cctttacata aagagcttgt tcttcttcga
    38101 ttgcttcctt ggcaaaggca taccacttgc tctgattttc tgtatctttt cctctatcac
    38161 ctaagccaaa agaaatttga taatagtctg ctctatcctg ccatgcttgt tttgaaattt
    38221 caagttcttt caatcgttgg taagacatca aacctgtctt aacagcgtag cctactgtaa
    38281 agacagcaac caactgacac aatagggtta aaatcatcag gcgtttaagg ggtaatctcc
    38341 ccttaataac gggaactaat gctttgtaac tcaaactcat tagataaaga agcattagta
    38401 aaattgaaat cgccaataaa aacaacagat agaaactaat cccaaaacca taggtggcta
    38461 acaagatagg ataaaacaaa ccttgactaa aaagaacgac tcccccacct aggaaggaaa
    38521 ggagggctga tagaaggagc catttgatat cagtagataa agaatgcccc atgatggata
    38581 agagagtctg accagaaaag agttttatac ctgctgctct catttcctta atccgagtga
    38641 taatcactaa agcaaagaaa gataagccaa atattgctaa actaattaaa ataaggggat
    38701 ttagtaatat tcgaaaagca agaaaatagg gcggtatctt tcggtcagca cttgctttat
    38761 aacccaaatc tcctaattta tcggcaagct tttctttcgt caaggagcct gacaaaagga
    38821 gataactatt tagcggatca ctacgttcac gactttcttg gctagcttct tggaattctt
    38881 ttggtaaagt tccctgacca taagttgcat aagtaaagtg agtcgtccca tccttactcg
    38941 gctctacaat tcttctagct attaaactct gttctgagtt tgcaaaattc tccaattcct
    39001 gttcaaatac ctcacgcgtc ggttcctgag tatctttttt gacacgaagt aaagaaacgg
    39061 aatcatagct tgcatataaa tattgtggcg cacgtaagac aataatccaa gcaaggaaga
    39121 agctgagaaa aaaagttgat aataatatga atagtttctt catagtagac tccttgtaaa
    39181 caaaattccc cctgtaattt cttacaaggg gaacgattta aatcaatgaa cgattagtca
    39241 taatcacagt aaaatgctac ttgttctccc catttagtcc aaatccatgc ataggaggtt
    39301 tttccagcag aagcttctcc tttatctgaa tgactattcc atctacttac aagagaagaa
    39361 taatgatatt ttttaccatg atagtaattt gaaaaagccc aaccacctcc tgaaccttct
    39421 ccatacgtcc atactcctcc atctggatat tgtacagcag ctgatgcagc tcccaataat
    39481 gtaaaacttg aaataagagc tagagcaagt aatctatgtt ttttcgtttt cattttattt
    39541 tttctttcaa aaaaagcaca ccttgagcaa caatgcaaca aaataaaccc tcctctctct
    39601 tttgttgaaa ccgctttctt atgtgataaa aatatcattt ttattatttg ttgtcaagga
    39661 aaaaatcgaa ttttttagat attttactac attgcctcta tgaataatat tatatagtag
    39721 ttttatttta aaataatatg caaccagtac taaccaaata taaaatagat gccattaacg
    39781 aattttattc aagtttttcc cattcatact atccaagcaa aagagatgat gttatagtac
    39841 aaaacaattc aaactattgt aaaattccta gcaaaaaaga gagccaaaac tctctttttt
    39901 atcttctttt actttttttg actggcatga gtgtaatgtc tctaacacta aagtaagcta
    39961 ggatcaacat ggctattgct aggaatattt ctgttggtaa ttgaaaaatt ttcagaaaag
    40021 atagaaccaa taaaatcaag agtgccacta aaatacatac catagcgacg atattgactg
    40081 tccctttaat gctttctggt gtcgcaaata catagagtag gagcagtaaa attcctagga
    40141 ctaaatagac catctttctc tctttctagc tcttattcag ctgatttttt cttcttgtta
    40201 gctttctcac gctctgcttt gttaaggatt tgtttacgca aacggataga ctcaggcgtt
    40261 acttccatgt actcatcgtc gttcaagaac tcaagagact cttcaagtgt caagatacga
    40321 ggcgtcttga taacagctgt ttggtcctta gtagctgaac gaacgttggt catttgtttt
    40381 gccttcgtga tgttaactgt caagtcattt tcacgagagt tttcaccgat gatcattcct
    40441 tcataaacct cagtacctgg gttgacaaag atcgtaccac gttcttcgat agacatgatt
    40501 gagtaagttg tagccttacc agcatcgata gaaacaaggg caccacggtg acgtccacca
    40561 atttcccctg gaatcaatgg caagtattgg tcgaaggtat ggttcatgat accgtaacca
    40621 cgagtcattg acaagaactc agttgagtat ccaatcaaac cacgcgctgg aacaaggaag
    40681 accaaacgag tttgaccatt accagttgaa atcatatcca acatttcacc tttacgttca
    40741 gaaaggcttt ggataacaga cccttggtat tcttctggag tgtcgatttg tacacgttca
    40801 aatggttcac atttaacacc gtcgatttct tttacgataa cttctggacg agatacttga
    40861 agttcatagc cctcacgacg cattgtttcg ataaggattg acaagtgcaa ttctccacgt
    40921 cctgaaacag tccatttatc tggtgaatca gttgggtcaa cacgaaggga aacgtctgtt
    40981 tgcaattctg cctgcaagcg ttcttccacc ttacgagaag ttacccattt accttcttta
    41041 ccagcaaatg gtgagttgtt gaccaagaaa gtcatttgaa gagttggctc atcgatgtgt
    41101 aggattggaa gagcttctac tgcatctgtc ggagtgatgg tttcaccgac aaagatgtct
    41161 tccatacctg aaacggcaat caagtcaccc gctttggctt cttggatttc acgacgttcc
    41221 aaaccaaaga aaccgaagag ttttgtaaca cggaagtttt tagttgtacc gtcaagttta
    41281 gaaagggtaa cttggtcccc aaccttaact gtaccacgga agacacgacc gataccgata
    41341 cgtccaacga agtcattgta gtccaaaagt gacacttgga actgcaaagg ctcatctgag
    41401 ttatctactg gagctgggat atggtcgata atcgtgtcaa agattggtgc catagtcgct
    41461 tcttggtcag ctggatcatc tgacaatgaa gaagttccgt tgatcgctga agcataaacc
    41521 actgggaaat caagctggtc gtcatctgca ccaagctcga tgaaaagttc caagacttca
    41581 tccactactt ctgctggacg agctgatggc ttatcgattt tgttaacaac cacgattggg
    41641 acaaggtctt gttccaaggc ttttttcaat acgaaacgag tttgtggcat ggttccttca
    41701 taggcatcta cgaccaagac aacaccgtca accattttca tgatacgctc aacttctcca
    41761 ccaaagtccg cgtgtcctgg tgtgtccata atgttgatac gagttccgtt gtaagcaacg
    41821 gcagtatttt tagcaaggat ggtaatccca cgctcttttt cgatatcgtt tgagtccata
    41881 gcacgctctg ccaattcagt acgtgcatca agcgtttctg attgtttcaa taattcgtca
    41941 accagggttg ttttaccgtg gtcaacgtgg gcgataatcg caatgttacg gatatcttct
    42001 cttaattttg tcatgatttc ctctataata ttcaaaattt attttctaac tgaacgatta
    42061 taccataatt tcaaataaat aacataactc aagcaagtgt aaatgttttc actccgcttt
    42121 tcttttcacg tcaagccttt tcaaagcaag cgacttatga taagataggc acagtatgcg
    42181 tttagataat ttattagccc aagaaaaagt tagccgaaag gccatgaagc aagcactcct
    42241 cagaggggaa attctagtcg atggttgccc agcccgatcc ctagctcaaa atatcgatac
    42301 aggactacaa gaactccttt ttcaggatcg aatcattcaa ggctatgaac acacctatct
    42361 tatgcttcat aagcctgctg gtgtcgttac agccaacaaa gacaaggaac ttccgaccgt
    42421 catggacctg cttccatctg acatccagtc tgacaagctc tatgccgttg gccgactgga
    42481 ccgagataca acgggactcc tcctcttgac cgataacggt cccttgggct ttcagctcct
    42541 ccatccccaa tatcatgtcg ataagactta ccaagtccag gttaatgaac ttctaacacc
    42601 tgaccatatc caagcctttc aaaagggaat tgtcttttta gatggtactg tctgtaaacc
    42661 cgcaaaacta gagattctat ctgcaagtcc ctccctcagt caagcctcta tcaccatttc
    42721 agaaggaaaa tttcatcaaa tcaagaaaat gttcctctcg gttggtgtta aggtgactag
    42781 cctcaaaaga atccaatttg gggacttcac attgaaccca gatttagcag aaagtaacta
    42841 ccgccctttg aaccaaaaag agttacaaat cattaaaaac tatttagaga tgagtcgata
    42901 aaacaaaaaa agctttaaaa ctaaagcttt tttcttttat ttaccgaaaa attaaggcga
    42961 ttgctacaat ccagttaact acagaaatca caattcctaa gatattaaga atcttttcta
    43021 ttttatagtc taattgtgac tctttttggt atgaaatagc caagaccaat cctatgatac
    43081 ccaaaatcaa gcctacaatt ggaaataaca aaccaagaat aatcgacaag atacccacaa
    43141 aaagtggatt tttcttcttt tctttcatgt tctaagaact ctttaaattt tatacaaatt
    43201 aattatacta taaaacaata gcttcatcct atcattcgac taatttggaa ataaggttag
    43261 ctagtcttca ctttcccttt ccaagaatcc aagccataag aaaggatata aatctcagaa
    43321 aaaccttgtt ttttcaagta aagagctgca tttgtaactc gttgcgcacg ttggttctcg
    43381 tagagaagga caggtttatc tttacgaagg gctgcaagac tagttttcaa ctgacttgaa
    43441 ggaatattgc gtgcaccaag gatatgtttt ctgtggaatt ctgctgggtc gcgcaaatca
    43501 atcaattgac ccgtacgaat caaggcttca aactcctcat tgtccacaat tttagccgca
    43561 cgacgaatac gaagatagtt aaagcccatc cacgccaaca ttgctagtat aagtgcccac
    43621 aaaatccaag taaccattag ttcttttctc catttttctc aatataatcc aattctacct
    43681 tgtgctctct gcgaagaact gcttctgcct ctagatagtc taatttatcc atcaaccctg
    43741 catcgtaaat ccgagatagt tccaacttca tcagttcaat atcatataag cgttttccca
    43801 tgtaaacaat aataccaaat cgtttgagga attgctgcac atcatagaat gttttcataa
    43861 gactcattct agcaaaattt tgtgtttttt tcaagaagag actcacacaa tgctccttat
    43921 tttcctatct tctttggcga ttctaaggca agtgtggtac aataaaaaca tgagaattca
    43981 acaattacat tatattatca aaatcgtcga aactggctct atgaatgagg cagccaagca
    44041 actctttatc actcagccaa gtctttccaa tgctgtgaga gatttggaaa atgaaatggg
    44101 cattgagatc tttatccgca atcccaaggg aatcaccttg acccgtgatg ggatggagtt
    44161 tctctcttat gcccgtcagg ttgtcgagca aactcagctt ctggaggaac gctataaaaa
    44221 tcctgtcgcc caccgcgaac tctttagcgt ttcgtctcaa cactatgcct ttgtggtcaa
    44281 tgcctttgtc tctttgctca agaaaagcga tatggagaaa tacgagcttt tccttcgtga
    44341 aactcggact tgggagatta tcgacgacgt caagaacttc cgcagtgagg tcggggtcct
    44401 cttcttaaac agttacaacc gtgatgtttt aaccaagatg ctggatgaca atcacctact
    44461 agcccaccat ctcttcacag cgcaaccgca tatctttgtc agcaagacca accctctggc
    44521 aaagaaagac aaggtgaaac tgtctgattt ggagaatttc ccttacctca gctatgacca
    44581 agggacgcac aactccttct acttttcaga agagattctt tctcaagaac accacaagaa
    44641 atccattgtg gtcagtgacc gtgccaccct ctttaatctc ttgattggtt tggatggtta
    44701 taccattgcg acagggattt tgaacagcaa cctaaacgga gacaatatcg tttctatccc
    44761 actggatatt gatgacccga ttgagctggt ctatatccag catgagaaaa ccagcctatc
    44821 taagatgggc gaacgcttta tcgactatct actagaagaa gttcagtttg atagttgaga
    44881 aatgataaga accaatatgt aggctagcaa caacctgcac attggttctt tttacttata
    44941 attaaaagtt tcccctgcca acttatcagc tagcttggga aagagagtat aaaacttatg
    45001 ggctaggttc aacaaaatcg ggagattgag ttctcgttta ttttttccta taatcttgac
    45061 aatcttttta gccactgcat ctgcctctaa gaggaagcgg tcaaccgatt taagataagt
    45121 tccatctggg tcagcttggt caaaaaatcc tgttcggatt ggacctggat tgactgttgt
    45181 cacatagact ccatagggca taagttcgag tcgcagagca tttgaaaaac caatagccgc
    45241 aaacttggtc gctgagtaaa gactagactt gccagtagct attagacctg ccatgctgac
    45301 gatgttgatg atatgccctt tgctgctttc cttcatacga gccgcaaggt gacgagacag
    45361 attcatcagg gcaaaggtat tgacctcaaa catctggtga atatctttat cagcaatctg
    45421 gtcaaatccc tcaaaaatcc cgtaaccagc gttgttaatc aagacatcaa tcttgccata
    45481 gcggagataa agatcagtta ccagagcttc tagggctgaa tcgtcggtaa tatcaatttc
    45541 aatcaattct gcatgggaat aatttccgta gagttgggct aatttttcct tatttctacc
    45601 aagcaagatg agttggtcat tgggcaggag tttgaccatt tcttgagcta gaccaccgct
    45661 agctccggta atgagaatag taggcatact tatcctttct gtgactgcta gatttccact
    45721 tcttccaagt ctttgaccac atggacattt tcaaaaattg tggcagcgtc cttcttgagt
    45781 ttgcttatat cttttgagag gaaacgggca ctgatatggt tgagtaggag gcgtttggca
    45841 cctgcttcta ccgctacttg tgcagcttgc atattagttg agtgaccatg gttacgagca
    45901 attttttcat cacccttgcc ataagtggac tcatgaacta ggacatctgc attgacagcc
    45961 agacgcacac tggcatccgt ttttcgagtg tctcctaaaa tagtgataat cttacctgga
    46021 cgtggcgctg agatatagtc tgctgccttg atttcagttc cgtcttccaa aacaagatcc
    46081 tggccgtttt tgattttacc aaaaagcggg ccgaacggaa caccagcagc cttgagtttt
    46141 tcagcatcca gcgtcccttc tagatccttt tgcatgacac gatagccaac acagaaaata
    46201 gtgtggtcca gctcctctgc atacacagtg aatttatcgg tttcaagaat tttacccaga
    46261 gaatcttggt caaactcatg gaaatgaatg cggtagggca gacgagaacc tgacacacga
    46321 aggctggtta agacaaatga cttgattcct tgaggtccgt agatttccaa atctgtctgc
    46381 tcttcattgg cctgaaaggc acggctagaa aggaaacctg gcaaaccaaa aatgtggtct
    46441 ccatgcagat gagtaataaa gattttgctg accttacgtg gtcgaattgt ggtttccaga
    46501 atgcgatttt gcgtaccttc tccacagtca aagagccaaa cttcgttaat ctcgtccaaa
    46561 agtttcaggg cgagacttga aacgttgcgg gctttagagg gctgaccagc ccccgttcct
    46621 aaaaattgaa tatccattcg atactttcta attaatcaat atataacatg gctgtgcggt
    46681 tttccgatcg gaaatagcgt ttgccagaaa aagcagcagc ttcttgcaat aaatcctctt
    46741 ggctgtagcc tttgagacgt tttcgaccat cagccaatct ttccaaatca gtcaaagctg
    46801 tgagactttc taggctgaca acttcctcgt ccttgacagg cttcatgtaa atcttaccag
    46861 actcttcaaa gactaattga tgggagaaaa tttgcgcaat ttcaaagagc aagtcatccg
    46921 agattttctc ctcattttca gagaaaatcc gaccaaagcc gtcactctca taacaaaaac
    46981 caaaggattt accagacaga ttaagccgaa taaaaggctt attttctagg gtgaaacttg
    47041 gctcagtatt gtaaagattc agttcctgac tgagttctgc aaaataatcc gtcgcagcct
    47101 gaggactttt tttctgatag agttctgcaa agtaggcatt aacaacactt ggcggaggtg
    47161 taataagtgt taactgctcc tgctctgttt taccagctag aagctgatcc agatagacct
    47221 tgtccagact tgtataacct ccatacttta gagccaaagt tttaatatca gtcataaaat
    47281 tcttctaacc tccatttatt tttctcggaa atgtagcctg taatcacttc gccgtcttcc
    47341 tgataatcac gttcttccag aattgcaaca ctctctaaat catgaatctt gtaggacttt
    47401 gaaaaaggca ctcgcagggt aaatgcttca aaaatttcct taatcttatc tagcaataat
    47461 gcttgcaagt tttcacgact gtcctcagac ttggcagaaa tgagggtata tggcgtttgg
    47521 gtaggcgtga aatcctccac caaatccgct ttattataaa gcgtcaagtg aggaatatct
    47581 tccatgtcca ggtctttcat gatggagaga accgtttttt catgctcctc gtggtaagga
    47641 ttgctagcat cgataacatg aaccagaagg tccacatgct tgctttcttc caaggttgac
    47701 ttgaaactgg acaccaactc tgtcggcaaa tcttggataa agccaacagt atctgtcaaa
    47761 gttacttgga gattgcctcc cagatgaata ctcttggttg tcgcatccag agtcgcaaag
    47821 agctcatctg cttcatactg ggtcttactg gtcaagatgt tcatgatagt tgatttccca
    47881 gcattagtat aaccaatcaa accaatctta aaagtgctag actccaaacg tttttctctg
    47941 acagtcgcac gatttttctc aaccacctta agctggcgct cgatatccgt gatttgattg
    48001 cgaacgctac gacggttcag ctccagttgg ctttcaccag gaccacggga accaattccc
    48061 cctgcctgac ggctgagcat aatcccctga ccaaccaagc gaggcaagag gtatttgagt
    48121 tgggctaggt ggacttggag cttcccttca tggcttcgag cccgcatggc aaagatatcc
    48181 aaaatcaact gcatacggtc aatgacctta acaccgagaa cttcctctag attgacattc
    48241 tgccttgggg tcagacggtt gttgacgatg acagtagtga tttcttctgc atccaccata
    48301 agcgcaatct cttccaactt accagagccg acgaaggtct tggaatcata tttttcacgt
    48361 ttttgtctgt agctatctac aacgactgcc cctgccgttt tcgctaaact agccaattct
    48421 tccatggaga ggtcaaaact gtccataccc tgcaattcca caccaatcag caggactcgc
    48481 tcctcttttt tctccgtttc aatcatctaa aaactcctct atctggctta aaatgcggtc
    48541 ttgtacacca gattctccaa tctgataaaa ggtgacctgc atgcgattac ggaaccaggt
    48601 cagctgacgc ttggcaaaac gacgggtcgc ctgtttaaga ctctcacgag cttcctccaa
    48661 ggtctgctct ccacggaaat aaggaaagag ttccttatag ccaattcctt tagcagcctg
    48721 tacattaggg gaatggtcaa acagccactt ggcctcatcc aaaagcccag cctcaaacat
    48781 caaatccact cggtggttga tacgctcata aagttgacta cgttcatcat ccaagcagat
    48841 aatcagcggt tcatacaaaa tctcttgatt ttccaaatcc tgaccaaaat gggcaatttc
    48901 taaggcacgc atagcacgac gacgattaaa ctggggaatc tcaaggcctg cttgctccac
    48961 caaatgggct aattcctcat ctgaatatgg ctccaaacta gctcgataag ctaaaatctc
    49021 ctcatgagga gtctccccac ctaggtggta accttctagc aagctctgga tataaagtcc
    49081 agtcccaccg gcgataatgg ctagcttgcc acggctgtga atatcctcaa tagtcatctt
    49141 agcttctgaa acaaaatcaa aagccgagta agactcagtt atctctctaa catcgattaa
    49201 atgatgagga acagctgcct gctcttctgg actagccttg gccgtcccaa tatcaagtcc
    49261 tcgatagact tgctggctat ctccactaac cacttcgcca ttaaagcact ttgcaacttc
    49321 aatagctaga gccgtctttc caacagcagt cggtccaaca atcacaatta tttttgtttt
    49381 catctttttt ccttgaaaaa ttcccatttt ttcgttacta ttattataac acaaaaaggt
    49441 cagtcagaaa aatgtgacct cttgaggagg ctggctgatt gagaatataa aggaggaaga
    49501 ctcatggcta aaggattcgc taaaggtctt gtaacaggtg tcgcaggaac tgtcgctgcc
    49561 gttgcaggtg cagtatacgc atttaaaaag aaagtaatcg aaccagaaga gcaaaaagca
    49621 gctttcatcg aagaaaaccg taaaaaagca gctcgtcgcc gcgtatcacg ttaagaaaca
    49681 aaagaagttg ggattattct caacttcttt tttctattct tttaaatagc taggtcaaac
    49741 ttcaactgtg gcttaacagg gtcataatcc accaactcaa aatcttctgc tttgatatca
    49801 aagaaattag tcccatcagg aacatttaaa accaagcgtg gttggcagtt tgacggctcc
    49861 cgacggagca attcctgagc ttgttcaaat tgattatcat agatatggag gttgttgatg
    49921 aagtagaaga acttcccaac cttccagcca aaatgtttag caatcatcat ctgcaaagcc
    49981 acatactgca tagcgttgat gtggtgggcc accagcatat cattggagcg ctgggtcaag
    50041 gtcgcatcca gatagatttc cccatcaaca cgccgaacat caaacatggt ctgaaaggcg
    50101 cacgggagca gcccatctgt ttcttcgaaa gcttggtaat cccagagaga aataatattg
    50161 cggcggttcc aaggattggt ttccaactgt ttgagaagct tattgataat gtcgtgtttc
    50221 ttaacaacgg caccatagcg ctcaccaatg gttcccgtgt ctccaacttc ccagtcattc
    50281 cagtagtgaa cattgtactt gtcattaagc acttctaagc tatttgactg gtcttggtaa
    50341 atccagagaa cttctttgat ggcggatttg attgcaatgg gacgcaaggt tatgatagga
    50401 aattctcctt tagataagtc gtactcggaa aaggcaccag ttacatactt ggaattagca
    50461 acagtcccat ccttgtactt aggacgagcc tgctctgaga aaacaccttc ctttagaatg
    50521 cgttcaatat tctctttaaa aatcgtatct gcttttgtca tttactctca cctcattcat
    50581 tgtcctaact agtatagcat aaaaaataaa agaggaacat tactaactct aggttagcat
    50641 tttcctctct cattatctta ttgcaataca agtgatgctg ctccgataac tccagcgtca
    50701 tttcctagag ttgcaagagc taatttagta gatgtgcgta cttgtgggaa agtattttcg
    50761 tcatagactt tttgaacacc ttgtagaagg aattctcccg cagctgacac accaccaccg
    50821 atgacgattg ttgatgggtt taggattgaa ccaatgttag cacaagcgat tcccaagtaa
    50881 cgtgagaagt tacggtaaac aatcaaagca aggtcgtctc cttcttttgc gagatcaaag
    50941 acagtcttag cagttacttc ttctccgtta tcaatcaagc gtttcaaggc tgcatcgcct
    51001 tcgtattcat cggcatagcg acgagtcaag ttgacaattc ctgttgcaga agcgactgtc
    51061 tcaagacagc ctttcttacc gcaagtacat gagattggct ggtcaaagtc aacagtgatg
    51121 tgacccaact cacctgctgc accagcaaca ccgtgaagca atttgccttc tgcgacgata
    51181 ccgccaccaa caccagtacc gagtgtcata aagacaacat ctggttggtt atcaccagca
    51241 cccatccagc gctcaccaag agctgctacg ttggcatcat tatcgatgaa aaatggaatg
    51301 cccaaagctt tttcaatctt ttgtttgatt ggttgaaggg ttttccagtt caagttgtag
    51361 gcaccgataa cagtcccttt gtcacggtca accacacctg gtgatcccat tccaatgcct
    51421 tggaagtccg ctgctgccaa tccaagcaag tccaaacgat gttgaataga ctcaatcata
    51481 tcatcaacga tatgacttcc ctcatccaaa atgttggtct tgattgacca ttttccttgg
    51541 atttctcctg ctgttgttaa gattgcaaat ttgatagaag ttccaccaag gtcaatccca
    51601 ataatctttt gactcattat attctccttt ttcattgtgt agtaattgaa aatgcttaca
    51661 gtactagtat atcaaaatcc attcacttat gcaaaggttt gcgtaaatga attataagga
    51721 attttttcgc ctaccagtat ctgttaacca tctaaatgat caatggcata ttgagctgca
    51781 tcatttgaaa atttatcaaa atcagaagtt aattgtctat acatacgttt ttttgacatg
    51841 tggaataacg aattataact ttttgccttt cctaaagcag cagaatattc actattgcta
    51901 tcattactag aagaagctgt acttgcttga ccttccttcc aaactccgtc agcgccaaca
    51961 taataacgtc caccatcaac ccattcattg acagccattt taccatccga ttttagatag
    52021 taatttcctt gccatgtact ataagcataa gaaccatctg atgtcaaata ataccaagct
    52081 tgataggtgg catcataaac ccattcacct ttagccattt taccatctga tttcaaatag
    52141 tagtttcctt gccatgcatt gcgagcatat gaaccatctg atttcaagta ataccatgct
    52201 tggtaagaag aatcataaac ccactcacct tgtgccattt taccgtttga tttaaggtaa
    52261 taagctcctt gccatgcatt ttttgcataa gaaccatctg atttcaaata ataccaagct
    52321 tgataagaag agtcataaat ccattcactc tgtgccattt tgccatcagc tttcaggtaa
    52381 tagtttccct gccatgcatt tttcactaga tttccatttc catcaaaata gtaccaagaa
    52441 ccgtcctgct taacccaacc acttgctgtg gatgaagttg tatcagtttg agttgtagcc
    52501 gcttgttttg ctttaaaagc acccttagga gcatttttca attcgcaagc cacaccatca
    52561 tgatcgcggt ctaacttggc agaataacca ggttctccct catgaagatc cgaatatcca
    52621 ttagcccaag cttccttaca gctcgaaaaa tggatgtttt cttccgccaa aactggttgt
    52681 gaaaacaagg ctaaaattgg caacgtcacc agactcattt ttaaaaatag acgtttattc
    52741 atttctttct cccataatta tgatatcgat ttcaatatta ttttatcaaa cttatttcag
    52801 caattcaagg aaacttctgg tcttttctac aaactccttt ggttttgcct tattcaagac
    52861 atgagggcca tcaggaatga tctgaaactg cgaatctgtt aacagtctat ggagagcttt
    52921 catagaacta agattcggtt tatctttgct gccacaaatt agtaaggttg gataggagca
    52981 agtccctgct atatccgtta aatcaagtgt cttcaattcc tcagaaactc cgaccataag
    53041 agtcttgtct gctccctgtt tttcaaatac tcttttggga agtagtttaa aaatcagcaa
    53101 ctgaagataa aataggatat tccctgctaa tttaagcggg catcctgaca gaatcaaagc
    53161 tcgaagattt ggtaaatcgt aactggaaag ttctagtgtc agggcagcac ctaaggacaa
    53221 tccaatcaaa acaaaaggtt ctgtctcttg agctaggtgc tgataaactc gctctttagc
    53281 ttgttgatag ttactaactc cagaaggaaa taactcgata gcctcagaag gataatctgt
    53341 cagtagattc cgaacttctt tccaagactc tgctgactgc cctaacccat gcaaaaatat
    53401 caatttcatc tagttctcct caaggcttag ttcatacaag cctctcactg cattacagcc
    53461 gtaaatagct tctgcttggg ttaaatctgc caaggtcaag actttctctt ctacctgtcc
    53521 tgtttctagc aaatactgac ggtaaattcc tggtaagatt ccaagtcgga taggcggtgt
    53581 gtagagtttt ccagcgattt tcagaaccaa atttcctata gaggtttcaa gcagttctcc
    53641 tgacttattg tggtaaatct tctcttgttc tcctaggctc aaatgcggtc ggtgagtggt
    53701 tttaaagtag gtaaaggatt gattcaaagc agcttcctga aggcagactt gggcctgaca
    53761 aaagcttgta ctgagagggg ttaatacttg acgattgact tctatctctc cagatttgct
    53821 aaggctgatt cgcaagcggt aatctcgatt agcttcacaa tcctgacact cttcctcaat
    53881 cttgtgtccc aagtcttctg catcaaaagg aaaagcaaaa taacgactag cttttctcag
    53941 tctttccaga tgttgatctt caaacagcag gttcttctgg ctgatttccc ctgtcgtaat
    54001 cagttggaag cgagcttgtt tacgataaag aactgctgcc ttttgatgaa cctctcggta
    54061 ttcagattcc catgtgctat cccaagtaat ccctccgcca actccataaa tggcttgacc
    54121 tttgtgaagt tgaatggtac gaatggccac attaaaaatc cgtcgtccat ttggaagcaa
    54181 gagaccaatc gttccacagt agactccacg cggttgaggc tccaagtcct tgataatctc
    54241 cattgtcgca attttcggtg cacccgttat ggaaccacaa ggaaagagtg agcggaagat
    54301 ttcaacaagg tccacatcct ctcgcaactg actcttgatg gtcgaagtca tctgccaaac
    54361 agttgaatac tgctctacct gacacagacg ctccacgtgc tcgctcccaa cttcagaaat
    54421 acggttcata tcattgcgca agaggtccac aatcatcata ttttcagagc gatttttggg
    54481 atcctgttcc aaccaactgg cctgttcaag atcttcttgg tcagttaccc cacgctgagt
    54541 cgtccccttc attggtcgtg ttgtcaactc gcgatcattt tgctcaaaaa agagctctgg
    54601 gctcatggaa atcactgcca tctcgtcatg ttccacatag gcattgtagc ccgcctcctg
    54661 ctctaccacc atacgattgt agatggcaaa aggattggca cttaactttt gcttgagttg
    54721 gacggtgtag ttgacctgat aggtgtctcc ctgcctcaag tgatggtgta tctgggcaat
    54781 ggccttttca tagtctgctg cagacgtcac ttcctgccaa tttgagggca aatcaatatc
    54841 ctcataagtc agaggaatag gggaagtttc tacgctatca tgaacagtaa agtaaagcag
    54901 gtactctccc agtaggggat ccttgtgaac tgctaatttt tcctcaaaag caggtgcagc
    54961 ctcgtagctg acatacccca ccacataata accttgctct tggtagcttt ccacttgtgc
    55021 cagcaaatct gccacttctt ctacatttct cgttttcaac tctttaatag gctgggcaaa
    55081 ggtatatctc tcccctaaag tcctaaaatc aatcactgtt tttctatgca taccttaagt
    55141 atagcataaa aaggcagata cagacaaagc tgatctacct ttttacgatt ttattcattc
    55201 tatgcttgtg gtgcagtaaa tagatcacca gagaaaccgt ctgtgttttt aaggagagct
    55261 ttaaagactt tcgatttcaa gacaactgta tttcggtatt tgtctttaat actactatcg
    55321 tttagttccg ttaaatcttt agtaacagcc tcatcaaaca tagtcttcaa ctgttcatag
    55381 gttgaaattt gagtgccatt aatttcaatt ggtgcaaaac cttttttaga ttttgtaaga
    55441 acttccccat accattgctt cttccaatct tcaaggtttg aaaattggtt attagaaact
    55501 ttcttaataa tgaaatcatc acctaataga ccgttatttt ctttattggc tgcaccttga
    55561 tgtttgcttg aaacatagtc aatgaaccca tgttcatatc catagtaacc ccacatacgg
    55621 aaggcgttat gtttgaagga tagagaacca ggggcacctt gactcgtatt accgccataa
    55681 ataccagcca tcatattaac attgacatat gccgtaccaa agtcctctgg gtggaagacc
    55741 ttattacctg gatttccgtg aatggtcatg aaattattat ttaccaagtc atcgatggtt
    55801 tcaaggttag ttgttttctc agcgtctatc aaatcacgca ctttgtccca ttggtgagga
    55861 ccacttagtt tatttctgtc cattggtcga cgtgtttcgc gatcaatttt cttgaaccac
    55921 ttactattgt cacctttaac cttaggaaga acagcctcag cttctgcata atcgagcatc
    55981 atcatagctt cattgtagtt cttcatgtaa tggtcgattt ggtcacgtgt ttgaagtttg
    56041 gtaggatctg gattatacca ttggtttcca tcatttgaac gttcaaatgc catatttaat
    56101 ccaagtgcgc catattcacc ttgatgtcca tgttctgctg gagtttgtaa cataccttga
    56161 gcaaatgctt caagatctgt accctgtcga tgtctatgac ctcccagata tgccattcgg
    56221 tcatttacgt gggtagtctc atgtgtaaag gcagatgtgc catattcact tattaaatcc
    56281 gacataacaa aatatacagc atccataggc tgtggatcag caaagatgta agcatacgct
    56341 ccatacccat acttataatc atgatactta tcaatacgtc caaagaattc acgaataggt
    56401 gcataatccg gtttatcttt tcgatgacca aatctatcag gccaaccacc gggtgcacca
    56461 tgattatccc atacaggagt tggaactaca ttttgactct taagaagttt atcgcggaca
    56521 ttatcagtgg caaggcgtga ccaaaaatct aagtaattaa cttgctcttt agcacgttta
    56581 ttgatttcac tcttgaacga ttcacgtttt tcttctgtat ctttaccata cttttcaaac
    56641 gaactaaatg agatagtatt atatgttgaa atcataaata aatgtgcatc tttcaagttc
    56701 agaagcgata aaatcatgcg tccatgttgt ccgttattaa gtccttcata tagacgatat
    56761 tttttattag caaatgcagg gttagttgac ggttgttcaa ctacataagc attattttca
    56821 atagctttct taaaccaaac atttatgtct gtttcatttg tgaatagttt catattgtag
    56881 gtcaagaagt catgtaagtt tccagaaaga gtatttttcg ccaccacttc acggaaggac
    56941 tcctgtgtac gacttccctt aatatagtcc tcttttgaac caatttctat taatcgatct
    57001 aaaacactta cattcttacc ataaaaatct ggtttgaata gcattaattc tttaatattg
    57061 aagttatcaa atttcacacc gtaataacga tttaggtatg ttaatcctaa caggacagca
    57121 gctttgttat cttcaatttt cttaatcaag gcacgttttg cagcttcatc tgtattcagt
    57181 tgatgatctt cattttcaac taattttgtc acaaatgtat taagatgatc ttttacttct
    57241 ttaaagcttt cttctaagta aagattctta attgcattaa ctctgttatc cccagttctt
    57301 cctaatcgtt gataaattgg atcagattgt aattcaattg gacttaaaat accttcaata
    57361 gcactaatta aatctgcacg atctttaaca accatatttg gtgtatagac gacttcacca
    57421 agctctgcga cactgtattc ttttacttgc ttaacttgag agtctttagg agaaatcgta
    57481 aagatatctt ttgtcttatc tgcgtaatga accataatat ggtcagcatc tgtaaggtct
    57541 gtaataaagt cattaccttt cattgcagtc acagacaata cttctttatt cattaatgga
    57601 ctatcagttg taagcttgtt cccttgattc acaatccatt ctttgttgta gaacggttgg
    57661 agtttttcaa cattacgata aacaagctca cgatttgcat tataatcttg aattgttttg
    57721 tattcttcat cacgtaatat acggcgattc aacttatcaa ccaaatgctc agttacttca
    57781 aatgtatcag cagtgatacc catatcctta attttcttat cagcttctac ctgtgaaatt
    57841 tctttaattt tagacgattg tttatgggtt ttatctccag aactcacacc gataactgtt
    57901 gcattattag gtttaatcca attgcttaca aattcatcat ccatatcatt agaaccaaag
    57961 aaaatatcac cacgcttaac attcactaag ctgatattgt tatcaatggt tccccaaggc
    58021 cagtctttac caattaaacc gcctacatca cctctagcaa catcaatagt acctttggct
    58081 acagaatttc ttacattaga ccaagcacca acatctcttg gattaaaatc atgagctaaa
    58141 tgtcctacta tagcacctgc acgagtattt aaacctttaa gttcaacatc tgcataagct
    58201 ttatttaaat tgcctcgata aatctcagat gcaatcccag ccaagttcca ccctttatca
    58261 ccaacagatt gaacttttcc gataaaagca acattagtca taatacctgc atcatctaat
    58321 ttatccaaaa tacccgcaac attgttatta ccaacgatag tacccgttac tttaatattt
    58381 tcaacagttg catttttcac tgtttgagct aatgaagcta attgatttgc ccaaggcata
    58441 ttaatattaa cgttaattaa attaatattt ttgatagaac ccccttcaac tctatcaaat
    58501 aatggacgag cagtgttatg aatggagaaa tgcttaccat caacacttgt taagtgacct
    58561 gtaaatttac ctgttacata tgatttactt ggtgtaggta catttgttgc atttaaatcc
    58621 gatcctaatt taaactcacc gttaggattt ttctgcatag catctactaa atctttgaag
    58681 ttatagtaaa cgttattttg cgatgctttg ggtttctcta tgtaataagt gtatgtttga
    58741 ctcaaactat tatttgaatc acgttggatt aaatcaggag ctgaagcagt aactttaaat
    58801 aaggatgtct cctcctcagt tacttcctct atattagtaa tggcaagcct tgttacttta
    58861 ttatgattag tggtaatttt tagataatat gacgttagat tttctggaac agtttctaga
    58921 aggctactat ctgtttcatt tccattctca tccactttca tcaatcttgt ttctttaata
    58981 tctttaatct caactttttt aaggtctaac tgaacttctt tatcttctaa tatttcagtt
    59041 tcttcaccgt tacctcgatc atagaccatg gttgtggata gtgtataacc ttgataatat
    59101 ttcaagttgt ctaaagtagc ttgtaagtta ttttcttcta ccgttacttc tttaacaact
    59161 gttccagtat tatcttttag agctacttta atcgatttaa tttctactcc agctggttta
    59221 gtcaatgtat aactagctgt cgcactcttc ttcaagacat ctttatctgt tttagcccat
    59281 tccaaggttg gtttctctaa ctcttttgtg ccattcttaa taattttgtc ttgaggtttt
    59341 tcaatcactg tttctgtgat agtaggagcg tcacttgttt tttctccgcg aattgtttta
    59401 taggtttcag taatttgttt cttatcgttt acaccttctt gagttacttg tcttgttccg
    59461 cgttttaaag tgtcatcttc ttgttctttt gtttcaaaag gaatgatttc ttctcttgtc
    59521 tcaaccgttg tcccttcaat tggttttgta ccacggatta cctgttgatt tacaggctcc
    59581 ttggtgactt ccgtactggt tgaaagaact tggtcagttt taacccctcc tattgtttta
    59641 taggttgttt ttgtaacttg acttcctttt tcaccatttc gtaggacagt ttcttcgtcc
    59701 gtgtacttgg ttgggtcctc agtcgtttca gttttatagt caagttctgt agttgattct
    59761 tcaacgagtg ttccttcagt caccttatac tcaggtaatt gttctggaac aagagcctca
    59821 cccacgtaat cttcttcttg cgtcccctta gctgttttag tctcctcttt tggttctaca
    59881 gtttcttcag ccttggtatc tggagttgac tcttcttgtt tcggtgtctc ttccgctccc
    59941 ctatcttctt taggattgtc ttcttgtgat gattcttttg gtgtacttgg ttgctcagct
    60001 tgtgtaggtt gcacgggttg ttctgggact tttgcttgtt ctactggtgg ttcaactttt
    60061 gattcctcag ctggtttgtc tgatggttga ctttctggct taactgctac tttttcctct
    60121 gattttgact caacttctcc acctacttct tcaactggag ctggttctgc tgaatcttct
    60181 ttcccctctt ctactttagg aagggtgtcg tcagtaggtt ttacctccga ttttggttct
    60241 tcctttggac tttcttctgt tctaggtgct tcttcttttg gagtttcctc tgtctctact
    60301 acttggtttt ctgtcctagc ttgctcctga tttgttgttg attgaggagt ctcaacttcg
    60361 actacagtca cctctccagg ttttgctgag gtttcttcta aaacagtgtc caagccaagc
    60421 gttttgagga tgtcacctga tagataacca acatagcgat agccatccat ttcaacaaca
    60481 ccctctcgac tagccagcgc taaggtcgca actgggtcta cagcccctgc actaggaaga
    60541 actaccaatc ccatagctcc aactagaaag acgctagcaa ttttctttct cttgtagatt
    60601 aaaagcaagc tcccaacagt cagcaaacca aaagctgtca aaacagatgc ttctgtccct
    60661 gtttgaggca actgatcttt ttgatacacc aaaccatata caacttcatt cctgtcaggc
    60721 tttcctgtct gaattaaatc tttagcttct tgtgaaataa tctctttatt tacatagtga
    60781 taggtagctg cgtccactac agaaggagcc atcaaaaggc ttccaagaaa tacagagcct
    60841 acaactccct taatcttacg aattgaaaaa cggtcttttt taaacacttt catctccttt
    60901 attcattctc aagactttct aatagcatct tgcggatagt gcgcacgcgc acctccgatt
    60961 aattttggac gactagccag tgccgttaca tgggcatgac caatctctct caaaataggg
    61021 cgaatcggaa cctgaacatg cttgacatgc atgccaattg cagtgtctcc gatatccaat
    61081 ccagcatgag ccttgataaa ttcaacctca actggatcct gcataaactt aaaggctgcc
    61141 aactgccccg aacctcctgc atgaagagta ggatggacac tgacaatttc cagatcaaac
    61201 tgctctgcca cctgacgttc aacaacgaga gcccgattga catgctcaca accttgaact
    61261 gctaaatgga tacctctact acctagaata cccaagatag tctccactat cagctcacca
    61321 atctcttgac tggattcttt cccaatctga ccacctagca cctcactaga agatagacct
    61381 aaaacaaaaa ggaccccctg cttcaaattg gtcttttcta aaacatcttc cactacctga
    61441 cgtgtttctc tttgaatctg tgtctcgttc atctctgtta cctctgttgt cactcttcta
    61501 tcataccgtt ttttcttgtt tttagcaaga tagacaacct ataaagtctg cccaattacg
    61561 cataaaactc ccagaattga ctgggagtta gctagtttct attctattta tatatatttc
    61621 aactttcgtt cctttttggg gtctagaatc aatcttcatc tggtaattgg ctccaaaatg
    61681 aagtttgagc cgttgatcga cattttgaag accaactccc ccacgtttga gttgactttg
    61741 actactatca ccagcatctt ggaagccaac gccatcatcc tcaatacgga tgaccaatcc
    61801 cgaatcctgt ttctggacag aaagtttaat atggccctga ccttcctttt ccttaatgcc
    61861 atggtaaaga gcattttcta caaggggttg taggaccagc ttgggtaaga ctaaattatc
    61921 aaaggcaaca ttttcattaa tttcgtattc cagcttatct ccatagcgtt gtttctggat
    61981 aaagagatac tggcggacat gattgatttc gtcagagaga caaatcaagt ccttgccttg
    62041 attgagcgcc aagcggaaat aggttgccaa ggacttggtc acctgcacca ctcgctgact
    62101 atcatgaaat tcagccatcc agatgatggt gtccaaagtg ttatagagga aatgtggatt
    62161 aatctggctc gaaagggctt gaagttggta ctgacgggtc gtttcttcct ggctacgaat
    62221 agctaccatc aactgatcaa tctgatccaa catagcatta aattggcgag ttacttctct
    62281 cagttcatag gcaccaactt ccttggcacg aagattttga gcaccagaag caatttccaa
    62341 catggtttct ctcaaatcct tcaaaggagc aatccagcgt ttaagactga accacactaa
    62401 gcagagacaa acaagaagag atgtgacact ggccccaagc aaggtccaca agagctgact
    62461 ccgaacctgg tctaactttt ccaatgatga cacgccaagt accgtccaat cagttcctgc
    62521 aatcttctct tgactgacgt aggatttgtg accaggagta taaccctgac ctgtctcgat
    62581 gtagggtttc atagcctcca ttttgctaga cgaactataa actgtgtgtt gaggatggta
    62641 gacaaattca tggttttcat tgataatgaa ggcaaagccc tgctgcccca actggagttg
    62701 attgagatag gcttccagag tttcataaga aatatccaaa cgaagcacac caagattggc
    62761 tccctttgca tcaacaagtt cttgagtgac agaaatgacc cactgactat ctgatttacg
    62821 agctggagtc aaaacaggca tagctccctg atgaatagcc ttttggtacc aatcctcagc
    62881 catcatatca gaggaagttt tcatctgcac actgtcatct gtagaaatga cctgaccaga
    62941 tttggtcacc agcacaacag ttttcaagtc cttatctgac ttcaagatgg tcaaaaacaa
    63001 atctcggatt ccctcgacct tgtcttgact gggattctca gcataggcca gaacatccgt
    63061 ctgctgggtc aaaccagtcg aggtggtttc tagttttttg atataagact gaataaagtg
    63121 gctagtctgg ctgatggtcg tttggctgtt gccctcaatg gtggcctcaa tggctgaaga
    63181 acttgattga tagtagaaag ttccaaccag agctaggaga atgagaaaga ccagaaagat
    63241 ggaaataacc attctaacta aaagagaaga acgcttcatc ggtcttctcc cttcttaaac
    63301 tgacgaggtg tcacacctgc aatctgctta aaacgttggg taaaatagtt catatcttca
    63361 aaaccaacct tctctgcgat ctcataaatc ttcagatctg tagttaaaag caagagcttg
    63421 gcttgtttaa cacgttctct caccagataa tcctgaaaag gcaagcccaa ctctttctta
    63481 atcaaggaac tcagataggt cggactaaaa cctaagtcac tggctaaaga ctttaaacta
    63541 aattggctat cagccagatg agactggatt ttctgggcca tgtttccttc aaacctatta
    63601 gtcaataaat cttgtaactg ctcttctttc tcttccttgt ctagtttttg tttgattttc
    63661 cccaacattt cctcaatatc ctgacgagaa aagggtttga gcaggtagtc gtccacacct
    63721 agtttgacag cagacaaggc ataatcaaaa tcatcgtaac ctgttaaaaa gaccaaatga
    63781 acctgaggat aggtttctcg taccagactg gccaactgga tgccatttag atgaggcatg
    63841 ttgatatcgg ttaaaatgat atctggcacc tgcttttgga tcaattccca agcctgcctt
    63901 ccattttcag cctgaccgat gatttccata tcgtaggctg ctacattgac cagtttagtc
    63961 aaaccttgtc ttaccagata ttcatcttct acgattaaga ttgtgtaggt catgctctgc
    64021 tcctttacca cttactagta tcagtatagc aaaattctcc tctaactgct taggaaagac
    64081 ctcttatact caataaaaat caaaaagtaa actaggaaga tagccacagg tttctcaaag
    64141 tatagctttg aggttgtaaa taaaactgac gaagtcagat caaagtatag ctttgaggtt
    64201 gta
//
LOCUS       DAVBCB010000005        51676 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVBCB010000005 DAVBCB010000000
VERSION     DAVBCB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 51676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.005"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(54..362)
                     /locus_tag="VQU28_000289"
                     /pseudo
     CDS             complement(54..362)
                     /locus_tag="VQU28_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
     gene            complement(604..900)
                     /locus_tag="VQU28_000290"
     CDS             complement(604..900)
                     /locus_tag="VQU28_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475714.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYSKMK"
     gene            complement(884..3043)
                     /gene="pfbA"
                     /locus_tag="VQU28_000291"
     CDS             complement(884..3043)
                     /gene="pfbA"
                     /locus_tag="VQU28_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HET2475715.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVISTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKESVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(4173..5399)
                     /locus_tag="VQU28_000292"
     CDS             complement(4173..5399)
                     /locus_tag="VQU28_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HET2475716.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVFEVGPGDEVIVPAITYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5442..6134)
                     /locus_tag="VQU28_000293"
     CDS             complement(5442..6134)
                     /locus_tag="VQU28_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2475717.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGNLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSTEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFKVLK"
     gene            complement(6320..8071)
                     /locus_tag="VQU28_000294"
     CDS             complement(6320..8071)
                     /locus_tag="VQU28_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475718.1"
                     /translation="MQNKQEQWAVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALVDERTYEPLQENGQA
                     KVQEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(8061..9806)
                     /locus_tag="VQU28_000295"
     CDS             complement(8061..9806)
                     /locus_tag="VQU28_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2475719.1"
                     /translation="MSIIQKLWWFFKLEKHRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSPAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTELRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSEVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILNPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADFILVLQNGQIIERGRHEDLLAMDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(10012..10308)
                     /locus_tag="VQU28_000296"
     CDS             complement(10012..10308)
                     /locus_tag="VQU28_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HET2475720.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10461..10917)
                     /locus_tag="VQU28_000297"
                     /pseudo
     CDS             complement(10461..10917)
                     /locus_tag="VQU28_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            11051..11878
                     /gene="xth"
                     /locus_tag="VQU28_000298"
     CDS             11051..11878
                     /gene="xth"
                     /locus_tag="VQU28_000298"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HET2475721.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11890..12033
                     /locus_tag="VQU28_000299"
     CDS             11890..12033
                     /locus_tag="VQU28_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2475722.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRSEENFLFLLK
                     SVN"
     gene            12033..12272
                     /locus_tag="VQU28_000300"
     CDS             12033..12272
                     /locus_tag="VQU28_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HET2475723.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12656..12751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            13151..13732
                     /locus_tag="VQU28_000301"
     CDS             13151..13732
                     /locus_tag="VQU28_000301"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HET2475724.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13732..14994
                     /locus_tag="VQU28_000302"
     CDS             13732..14994
                     /locus_tag="VQU28_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HET2475725.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(15081..15947)
                     /locus_tag="VQU28_000303"
     CDS             complement(15081..15947)
                     /locus_tag="VQU28_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HET2475726.1"
                     /translation="MKQTRNFDEWLSTMTDTVADWTYYTDFPKVYKNVSSIKVALNIM
                     NSLIGSKNIQEDFLDLYQNYPEILKVVPLLIAKRLRDTIIVKDPIKDFYFDFSKRNYS
                     IEEYTMFLEKSGIFDLLQNHLVSNLVDYVTGVEVGMDTNGRKNRTGDAMENIVQSYLE
                     AEGYILGENLFKEIEQNEIEEIFSVDLSAITNDGNTVKRFDFVIKNEQVLYLIEVNFY
                     SGSGSKLNETARSYKMIAEEIKAIPNVEFMWITDGQGWYKAKNNLRETFDILPFLYNI
                     NDLEHNILKNLK"
     gene            complement(15934..16704)
                     /locus_tag="VQU28_000304"
     CDS             complement(15934..16704)
                     /locus_tag="VQU28_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2475727.1"
                     /translation="MTKPYYNKNKMILVHSDTFKFLSKMKPESMDMIFADPPYFLSNG
                     GISNSGGQVVSVDKGDWDKISSFEEKHEFNRKWIRLAKEVLKPNGTVWISGSLHNIYS
                     VGMALEQEGFKILNNITWQKTNPAPNLSCRYFTHSTETILWARKNDKKARHYYNYDLM
                     KELNDGKQMKDVWTGSLTKKVEKWAGKHPTQKPEYLLERIILASTKEGDYILDPFVGS
                     GTTGVVAKRLGRRFIGIDAEKEYLKIARKRLEAENETN"
     gene            complement(16730..17584)
                     /locus_tag="VQU28_000305"
     CDS             complement(16730..17584)
                     /locus_tag="VQU28_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HET2475728.1"
                     /translation="MKIKEIKKVTLQPFTKWTGGKRQLLPVIRELMPKTYNRYFEPFV
                     GGGALFFDLAPKDAVINDFNAELINCYQQIKDNPQELIEILKVHQEYNSKEYYLDLRS
                     ADRDERIDMMSEVQRAARILYMLRVNFNGLYRVNSKNQFNVPYGRYKNPKIVDEELIS
                     AISVYLNNNQLEIKVGDFEKAIVDVRTGDFVYFDPPYIPLSETSAFTSYTHEGFSFAD
                     QVRLRDAFKRLSDTGAYVMLSNSSSALVEELYKDFNIHYVEATRTNGAKSSSRGKISE
                     IIVTNYEK"
     gene            complement(17741..18145)
                     /locus_tag="VQU28_000306"
     CDS             complement(17741..18145)
                     /locus_tag="VQU28_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HET2475729.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(18215..19696)
                     /locus_tag="VQU28_000307"
     CDS             complement(18215..19696)
                     /locus_tag="VQU28_000307"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HET2475730.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEEAVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIVYRVPSDYGELEITINLSKPEKDPKEIAVAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSADHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(19715..20893)
                     /locus_tag="VQU28_000308"
     CDS             complement(19715..20893)
                     /locus_tag="VQU28_000308"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HET2475731.1"
                     /translation="MAQHLTTEALRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGAGLSSSASLELLTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVFENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            21058..22065
                     /gene="galR"
                     /locus_tag="VQU28_000309"
     CDS             21058..22065
                     /gene="galR"
                     /locus_tag="VQU28_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HET2475732.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFRNYSQAKGIYHDELVFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHDRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(22111..23148)
                     /locus_tag="VQU28_000310"
     CDS             complement(22111..23148)
                     /locus_tag="VQU28_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2475733.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAVEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLQSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(23149..23502)
                     /locus_tag="VQU28_000311"
     CDS             complement(23149..23502)
                     /locus_tag="VQU28_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HET2475734.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(23746..24636)
                     /locus_tag="VQU28_000312"
     CDS             complement(23746..24636)
                     /locus_tag="VQU28_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HET2475735.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            24769..25305
                     /locus_tag="VQU28_000313"
     CDS             24769..25305
                     /locus_tag="VQU28_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2475736.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(25472..26278)
                     /locus_tag="VQU28_000314"
     CDS             complement(25472..26278)
                     /locus_tag="VQU28_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HET2475737.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(26623..28143)
                     /locus_tag="VQU28_000315"
     CDS             complement(26623..28143)
                     /locus_tag="VQU28_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HET2475738.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPNLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLAALIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLVQKEKENLVIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGKTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMEPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(28136..28864)
                     /locus_tag="VQU28_000316"
     CDS             complement(28136..28864)
                     /locus_tag="VQU28_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475739.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            complement(28879..29643)
                     /locus_tag="VQU28_000317"
     CDS             complement(28879..29643)
                     /locus_tag="VQU28_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HET2475740.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVVDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(29647..30090)
                     /locus_tag="VQU28_000318"
     CDS             complement(29647..30090)
                     /locus_tag="VQU28_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2475741.1"
                     /translation="MKDSHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKFQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(30772..31068)
                     /locus_tag="VQU28_000319"
     CDS             complement(30772..31068)
                     /locus_tag="VQU28_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2475742.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(31090..32154)
                     /gene="pepA"
                     /locus_tag="VQU28_000320"
     CDS             complement(31090..32154)
                     /gene="pepA"
                     /locus_tag="VQU28_000320"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HET2475743.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            32555..33256
                     /gene="galE"
                     /locus_tag="VQU28_000321"
     CDS             32555..33256
                     /gene="galE"
                     /locus_tag="VQU28_000321"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2475744.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYKVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQNDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            33679..34638
                     /locus_tag="VQU28_000322"
     CDS             33679..34638
                     /locus_tag="VQU28_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2475745.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKEISESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLGFILLLWRGGRKDAD"
     gene            34628..35584
                     /locus_tag="VQU28_000323"
     CDS             34628..35584
                     /locus_tag="VQU28_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HET2475746.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLTLQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRSRQ"
     gene            35581..36333
                     /locus_tag="VQU28_000324"
     CDS             35581..36333
                     /locus_tag="VQU28_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475747.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGS
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            36429..37394
                     /locus_tag="VQU28_000325"
     CDS             36429..37394
                     /locus_tag="VQU28_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2475748.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEVTI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPAILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(37619..37861)
                     /gene="yidD"
                     /locus_tag="VQU28_000326"
     CDS             complement(37619..37861)
                     /gene="yidD"
                     /locus_tag="VQU28_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HET2475749.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(37861..38583)
                     /locus_tag="VQU28_000327"
     CDS             complement(37861..38583)
                     /locus_tag="VQU28_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2475750.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            complement(38570..39139)
                     /locus_tag="VQU28_000328"
     CDS             complement(38570..39139)
                     /locus_tag="VQU28_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HET2475751.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKSQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            complement(39158..39886)
                     /locus_tag="VQU28_000329"
     CDS             complement(39158..39886)
                     /locus_tag="VQU28_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HET2475752.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            complement(39886..40626)
                     /gene="xerD"
                     /locus_tag="VQU28_000330"
     CDS             complement(39886..40626)
                     /gene="xerD"
                     /locus_tag="VQU28_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HET2475753.1"
                     /translation="MPMRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            complement(40617..41078)
                     /locus_tag="VQU28_000331"
     CDS             complement(40617..41078)
                     /locus_tag="VQU28_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2475754.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            complement(41075..41596)
                     /locus_tag="VQU28_000332"
     CDS             complement(41075..41596)
                     /locus_tag="VQU28_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HET2475755.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            complement(41572..42543)
                     /locus_tag="VQU28_000333"
     CDS             complement(41572..42543)
                     /locus_tag="VQU28_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HET2475756.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGKSSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            complement(42540..43334)
                     /gene="racE"
                     /locus_tag="VQU28_000334"
     CDS             complement(42540..43334)
                     /gene="racE"
                     /locus_tag="VQU28_000334"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HET2475757.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIYVEHVEL"
     gene            complement(43541..43789)
                     /locus_tag="VQU28_000335"
     CDS             complement(43541..43789)
                     /locus_tag="VQU28_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HET2475758.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            complement(43931..45556)
                     /gene="treC"
                     /locus_tag="VQU28_000336"
     CDS             complement(43931..45556)
                     /gene="treC"
                     /locus_tag="VQU28_000336"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HET2475759.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTGNGIGDFRGIIGKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCIGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKDINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            complement(45734..47701)
                     /gene="treP"
                     /locus_tag="VQU28_000337"
     CDS             complement(45734..47701)
                     /gene="treP"
                     /locus_tag="VQU28_000337"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HET2475760.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGNDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            47886..48596
                     /gene="treR"
                     /locus_tag="VQU28_000338"
     CDS             47886..48596
                     /gene="treR"
                     /locus_tag="VQU28_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HET2475761.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            complement(48629..49432)
                     /locus_tag="VQU28_000339"
     CDS             complement(48629..49432)
                     /locus_tag="VQU28_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2475762.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKHLSVRKRAKDIARVFQDPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKLKLLLLDEHTAALDPKTSQMVMDLTQKIVEYHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(49445..49873)
                     /locus_tag="VQU28_000340"
     CDS             complement(49445..49873)
                     /locus_tag="VQU28_000340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475763.1"
                     /translation="MKTEIGLVLRSTGDNIPMSEANGVNVNTMKIVGYMISTGLIALC
                     GSLFAQNDGFSDVTSGTGTIVVGLSSEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYE
                     IPNLDQNLVRLFNAILLALVLFAPALQKRLKIRGLKLRNE"
     gene            complement(49882..50322)
                     /locus_tag="VQU28_000341"
     CDS             complement(49882..50322)
                     /locus_tag="VQU28_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2475764.1"
                     /translation="MNFVLSSLSEGLLWSIMAIGVYLTFRILDIADMTAEGAFPMGAA
                     IVVSQIQAGANPWLATLLALLAGMVAGLISGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCLLLVWF"
     gene            complement(50367..51272)
                     /locus_tag="VQU28_000342"
                     /pseudo
     CDS             complement(50367..51272)
                     /locus_tag="VQU28_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT        15175 a        11736 c         9477 g        15288 t
ORIGIN      
        1 attaaaaggt taagtaattt ttccaaaata cagcactcat atagacagta tcatcaatta
       61 tagccattgg gattttttga ttgccaattc catgtatctc tacacatgtc ttcaatcgat
      121 tttgttgtcc tccaatttaa ttccttatat gctttgtctg catttgcata ataagttgca
      181 acgtctcctg aacgtcttgg aactatttta taaggaatag ggatcttatt aacactttca
      241 aatgtattta caagttgtaa tacactagtg ccttctcccg agcctaggtt atagatataa
      301 acatctgttt tttcagatac tttttctaaa gcttttatat gtcctattgc taaatctact
      361 acttgaatct tttatctcat ttttcccttt ttcaaataag ttatctactc tatgatcagt
      421 tatttccatt tcgtttacta acatgacgca gtcacctagc atcatatact ccaacttttt
      481 ttctgaaagt ttagcaaata ttggtaagcg ataatatagt atattgaaac tagaatagta
      541 cacctctact tctaaaacat tgttagaaat cgatttgact gtcctgattg atttgtccta
      601 ttattatttc attttactat actctgttaa ttcatatgag tttaaaccga tttcatcttt
      661 aacctcgagt aaagcagttt caaatatttg tttaagagtt tttgattctt tacaattaac
      721 cgacaaactt tctgataaaa tatgtacaac ttctgagact gaataaccta tctcctcttt
      781 agaattatat aaatctgtag ctccaccaat aatccaaaaa tactgatttt gtgaacctac
      841 aatatcctca ttttctacgg aacttccttg tatcgaacaa attttattta tctttaccat
      901 aatacttcaa cccttttagt gtcaaaagta aaccaattcc tgtcactgtt aagaatagtt
      961 ccataatctt gttcgaacca gtctttggta atttttgttt tacatctact atttctttag
     1021 atttattaat atgattttca gtttctctgc catctccaac tattttatag tttacttctt
     1081 ctgtcttatt atcttgttta ttgtcgatct tgtcattcat ttgtctatta tctttacttg
     1141 agttaaactc tccgttcttc tggttactat caattacatt atttgaatta gattgttttt
     1201 cctctttgtt tttttctttt tcgtttttat cacttaaatt atttgttaca attttgtaaa
     1261 gcccattctc cgttacaata ttgaaattac catcgctatc acgtataaca gattctttcc
     1321 catttgcatt agatttgatg aatgatatat acttaccgga taaattataa aattggttat
     1381 ttaaaacggt tattttaccc tttgaatcct caataacaat tccttcttta ccaccgaata
     1441 tactattctc tgagactact aaattatcac taactcgtaa taattcaata tttggttgtt
     1501 ctgtttcctt agaattatta ttaattacat tttttgttac agtaatatct gatacttttc
     1561 ctaaatattc tgcactatct tttgcaactc gtatcgcttt tgttttagga tcggcaatat
     1621 taaatatatt ttcggcgata accacctgtt tatttaaatc taataggtct ttagtgtttt
     1681 tatagctata agcatttact aaagctgctc cgctttctcg ataatgtaca ctctctcctt
     1741 taactttctt atcaaagcga tttcctttga ttaatacatc agtgaatcct gtaaaacgta
     1801 cacctgcata catcatgtta tcaaaatgat tatttaaaat tttaatatta gaggggttct
     1861 gtgtcgacaa tgtttgatag tgtgtgccaa ttgctgttac taattcccca gatttatcac
     1921 ttttgccaaa ataggaattt tgaatagtca cattttcaga ttttttccca tcatcattca
     1981 aggcataagg aaaacctttt ctagttaatg gttcaatctg aatgctctcc ttacttatga
     2041 tttgcccatc cttcatcgtt ttgggtaagg cttgcccaag aaaacgagaa ttatcaacta
     2101 atacattttt cgaacctgca atttgaatag catgcccttg ataactatcc ttgaatgtta
     2161 cattttttat agttacgtta tttgaattcc caatagcaaa tgcacctgaa gaatttataa
     2221 gtggtagatt ttttgcttta gttccttctt cattcaaagc accgttcata tcaatcgtac
     2281 caccagaata actaatatct tctgttgggc cccattctac ttgcgcacca tcatccgtaa
     2341 ataaacctgt cataaaaaca atggaagggt gattcttaat atttatacca tttagaattg
     2401 tagctttctc attcaattct aagtgtgtat gactttttaa aaaaacaatt tcttttacta
     2461 aataagttcc ttcaggaaaa tatacatttc ctccacctag cccttgagct gcagcatcta
     2521 ttgcatcttg aattgcttgt cgatcatcat gaatcccatc acctaccgca ccataatctt
     2581 ttacacttaa agcagtatct tcaaattctt ttttgataat tctgtcactt ataacatctt
     2641 ccttataact cttattttca ttagtagata ttactgtaga ttcaactgtt ctgttgtcta
     2701 attgtgaatt ggaacgaata gtaccattag agctgatact attaacactt aacgaattat
     2761 ctaaaccatt agtggttgaa ttagaggcaa tcatttcagc agaacgattt tgattaacag
     2821 ttggtgaatt atctaaatca ttagtgattg cattagaaga ctcttttgta gccatcggtg
     2881 aagaactagt aacaacttca tccgataaaa taccttggct tcccaaaatt gaaattgcca
     2941 atagtaccga gcaagtcccc acctttaatt tacgaatact gaatacctca ttaggaacaa
     3001 aatatttcat cagaacctcc aattttttta ctatttttaa caattgatta ttacttttct
     3061 taaaatagat agaatgacac cgctgtcatt atctttatca tatattttcc aaattgaatt
     3121 gtcaaggaat aacacaagaa aatattaaaa tttatactct tcggaaatct cttcaaacta
     3181 ggtcagtgcc gccttgccct aagtctaggt tactgacttg gtcagttcta tagtagactg
     3241 aaactataat agtacacctc tacttctaaa acattgttag aaatcgattt gactgtcccg
     3301 atcgatttgt cctattctta tttcatttta ttatatctac aacctcaaag cagggttttg
     3361 agctatctat ggccaacttc ctagtttgct ctttagcctt ccattcaaaa gtgatgctat
     3421 ctttcttagc ttgaatataa aatatcccat ttactatatt taaatttgtt gatatgttgt
     3481 aaattataaa tgaaagaaca aatatcttgt catacaagca ttcgccaggg atatttacat
     3541 gaatatcgct catatggcgg attgaaacta aaacactaca atatatttct taaatagacc
     3601 ttctataaaa gctagattta acctcgataa aaatagatgc agtgcttcta aaatactgga
     3661 atagggcata taggtaatcg tagacaagtg ttttatagat tgtctcgcgg gagatctaat
     3721 gatgtatagc aagcatcact aaacatatag aatatttgtg ttattataat gtcaatatgc
     3781 tataaattct atcatgcaaa agaatgacta gaacatgaaa gacaggcaca aaactttgaa
     3841 caatattctg aaaacaaaaa aagacgcgag tttccacaat ctcactcatg gaaactcacg
     3901 tctttcaaaa aatagggtga attaaaaact agaacaagac actagatttt ctaaatgatg
     3961 tctagtaagg attctaaatg ctacaccaaa ttgctcacat cttatttcaa accacgggac
     4021 tcccacaaga aaggaaaatc ctataaaaat tctctctctg aggagagaga ggcctcaact
     4081 acaggtgtaa actattatca attgaaaaca acagttttta cactcaatta gactttttag
     4141 ggacaggatc actttcgctt gactgtagtt tgtcattttt ttgataaagt tagcactttt
     4201 tcagaaactg ttttgaaagt ctcaatgata tagtctactt cttcatcgct taatttagta
     4261 tgaagaggga gggtaatttc attctcaaag aaggcatagg ccttaggata gttcgtcata
     4321 tcaaatccaa gattcttata ggctgtcaag agaggaagcg gtttgtagtg aacattactt
     4381 gcaattcctg ctttagccaa ttcttggatg atgaggttgc gttcttctag gcttgctcct
     4441 tctacacggg tgatgtagag gtggcgtgaa gattcgacag tttcagtctt gtgtgccaaa
     4501 ggatggatgc gagaacctgc aaaaccacta tcatagcggt ccacaatgtc cttacggcgt
     4561 tgcaacaaac ttggatagcg gtccaattgt accaaaccaa gtgaagccat gatatcggtc
     4621 atgttgcact tataggctgg tgtaacgata tcgtattccc atgaccccag ttgcatcttg
     4681 gcaagagcat ccttagtttg cccgtgaagg gaaaggattt ggaattcctt gtacatctct
     4741 tcgtcatcaa tcactggatt ggctttccaa gtcgcacttc caccttctgc cgttgtaaag
     4801 ttcttaacgg catggaatga gaaggaagta aagtcagcga tagaaccaga aggttgtcct
     4861 ttataagtag atcccaaagc gtgggcacta tcagagacaa tgacaatacg gttaaaggcc
     4921 ttttgccact tgcttgaagc ggtaaagaag tcacgttttt tctccacgac ttggaacaaa
     4981 cggtcataat cgcaaacaat ccctgcgagc tctactggga taatcacctt agttttctca
     5041 gtgatagctt gctcaagcag gtcatagtcc atctcaaacg tatctgcttg gatatccacc
     5101 atgacagggg ttgctcccac gtgcgtaatg acactacatg aagccgtata ggttatggct
     5161 ggaacgatga cttcatcacc aggtcccact tcaaaaacgc gtaaaatcaa ctccagagcg
     5221 gctgtcgcag agttgagaca aacagtctta ggtgtctgtg tgtaaagaga caagcggcgc
     5281 tccagttctt ttgttttagg acctgttgtg atccaaccag aacgcagggt atccgctact
     5341 tcagcaattt ctgcttctgt gatatcaggc ggtgaaaatg gaatattgta atttggcatt
     5401 gatttgctcc ttttatctgt actcattctc ttatgactac tttattttag taccttaaac
     5461 acggtttgaa acatgatttt gatgtcccca aagaaactaa actctcggag ataggcgagg
     5521 ttatagcgca tcttttcagg aagaacatgc tccacatagg cctgatcaac tgacagacct
     5581 ttctctgtca tttgactgat gatggtgtcc tcatccttgt agttgatgct ggctggagag
     5641 gtaattcctg cttgcaagag caaggttgcc atcatttcag tgctatactg ctctgtataa
     5701 cgtggcactt caggtcgtgt accgacaaag gacatctcac ctttaaggac attgaccaac
     5761 tgaggcagtt cgtccaaacg gacacgtcgg atgaaatttc caaccttggt aatacggcta
     5821 tcgttagcag aagtcaccag atttcctttt ttatccgcat ccgtcaccat ggtacggaac
     5881 ttccaaatct tgaaccgacg gttgtactgg gtcacacgct cttgcttgta aatcactggc
     5941 cccttgctat ccaacttgat ccaaatgctc aagatgagaa agatgggaga ggtcagaacc
     6001 agtaagacca aggccaaaac ccagtccaag caacgcttga aaatcagcga acccttcctt
     6061 ttagagacaa gctggtagta agactcaacc tcgcttgatt tcatttccac aggcaagtct
     6121 tcccatttca gcatgctgtt ctcctttgtt tttattatac tatttgtcaa cactttttgt
     6181 caacactttt cattatacca caaaatggaa aaggcggtga aaaaaatctg taatttgaag
     6241 gatttccttc tagtactcaa tgaaaatcaa agtacagctt tgagattgca gataaagctg
     6301 acatggttta aagagatttt caaagagtat aggccatggc ccctgcctgc aaactataca
     6361 tcttgtgata ggttcctccc agagccaaga gttcctcatg ggttccactc tcgataatgc
     6421 gtcccttatc caagacatag atgcagttgg cgtcttgaat agtagaaagg cggtgagcta
     6481 tagcaatagt tgttcggccc tgtctcatct tcgccagaga agcttgaacc aagctttctg
     6541 tttcagagtc aatattggct gtcgcttcat ccaaaatcag gattttaggc tggctggcga
     6601 ctgttctagc aaaggcaaga agctggcgtt gcccagtaga gaagctcgaa ccacgctcgg
     6661 aaacagggga gtcgtacccc tgaggaagtt cttgaataaa ggaatctgca tccacaaagg
     6721 ctgccgcagc ctgaacctgc tcatcactga tttcttggta catggcgata ttggacttaa
     6781 tagttccatg atagaggaag ggttcctgca agaccaaacc gatgtttttt ctcagctctt
     6841 cttgactgaa atccctgata tccacatcat ccaagagaac tctccctgac tggaattcat
     6901 aaaagcgcat gaggacattg ataatagacg atttccctga acctgtatga cctacaaagg
     6961 caatggtttc acccttatta acagagaaag aaatgtcatc cagaatcgga tgtttaccgt
     7021 catatgagaa acacacatgt tcaaagcgga tattgccttc ttggactttg gcttgcccat
     7081 tttcttgaag aggttcatag gtcctctcgt ctaccagggc aaagacacga cctgcagaaa
     7141 ccatagacgt ttgcagtgtt gaaaagtttt gcgtcacctc aatcaaaggg tcaaagaggc
     7201 ggttgatgta ctggataaaa gcatacatgg tcccggccgt tatcccgata gaaagaccac
     7261 gatagccaaa ataggccatc aagacagcat aacctagaag tttcaacaaa ctcatggcag
     7321 gtctcaaaaa gagggcatcc aaggctacag aacggttggc atagaccaag tgctcttggt
     7381 tgatttcatc aaattctgcc tgcaggcgct tctcttgatt aaaggcctgg attattctga
     7441 ttccctcgat gttttcagcc agcttgctat tgatatctga caagagactt ctggttttct
     7501 caatgatttt cactgatttt ttccgataga gattgaccaa aaggaaaatc aaggggagaa
     7561 agagcaagac taatgctgtc aaacgaaaat ccaataccaa catggtataa agggttgtca
     7621 gaaagatgaa aactgctgag ataaagctag ataaaatccc agaaaacata tcactaatcg
     7681 tctcggtatc gttggtcaaa cgagaaacga tagaacctgc tggcgtcttg tcaaagtaag
     7741 acatgcccag tttctccata ttggcaaagg catcccgacg aatatcccta acaatactgt
     7801 aagacacgcg cgcaaagaga agattgccga catactgaac tacagtttgt aggatgtaga
     7861 gaccatagta gaccagcaaa acggtaacgg ctagttggtt aagattgctg agatactggt
     7921 cgataaagtg ggaagccacg aggggtatga cacttttaat gaccgtcgtc gctaggagaa
     7981 aactgagtgc caaaaaggtc aggagtctat aaggcttgag ataagacatc aagcgcttca
     8041 atacagccca ttgttcttgt ttattctgca tcttcttctc ctttcatttc taactgctga
     8101 gactggtagg tttgggcata ccagccatcc atcgctagca agtcttcgtg cctgcctcgt
     8161 tcgataattt gaccattttg tagaactaaa ataaaatctg catggacaac agcactgagg
     8221 cgatgggcag ttataatggt tgtcttgtcc tttcgcgtct ccttgagatt gtcgataatc
     8281 gcatactctg tcttggcatc tacggcggat aaggaatcat ccaaaatcaa gatatcaggg
     8341 tttaaaatca tagcccgact catagccaac cgttgctttt gaccaccaga aagactgact
     8401 cctttttcac caatcagcgt atcaaatcct tgaggcatgt ctacaatatc ttggtaaact
     8461 cgggctagct tagtagcttc ctcaacctct gaaaggggca agttaggatt gccaaagcgg
     8521 atattgtcta ggattgaagt cgcaaagaga aactggtcct gaggaacata gcccatgaga
     8581 ctgcgaagtt ctgtcaaacg atagtcccga atatcgtgac cgtttagata aatggcaccc
     8641 ttatccacat cgtattcacg caagaggagc ttgattaagg acgttttccc agagcctgtc
     8701 tgcccaacca agcccagtgt ttgccctttt gccaaactaa agtgaatatc cgtcagtgtt
     8761 tcctcatttt caaaagcaaa gctgtcaatg gcatactcca aacgcccatt ttcaatacca
     8821 tccagaggaa actcagggtc ttgtacagga gattcctgag acaaaagatt ttcaatccgc
     8881 tggtaagaaa ccttccctcg ctgagtagta ttaaagagga agccgatggc cataagaggc
     8941 cagaccagca tatccaaata gctgataaag gtgactagat taccaactgt aatctgccct
     9001 tcctgaacca tcaaggagcc aaccaaaagc gttaaaacat aggacgaacc aacaaacaag
     9061 agaaccatag ggtcaaagag actatcatat ttcatggttt gcaggttctt ttggaaggtt
     9121 aattcattga ctgcctgaaa agacttcaac tcgtctgcct gataaccgaa agacttggtc
     9181 actttgatac ctgatacgga ctcctgtacc ttgttattga gttcagaaaa agcagcttgg
     9241 gattcgccaa aggccttatg agtctttctc cctaggcgac tagtcgcata ggccatgaaa
     9301 ggtaggggga gaatggcaac aagagtcatc tgccatgaga tgctaaagag catggtcaac
     9361 aaagtcacca gagccgtgat agaggcatcc accgcagaca tgacaccgcc acctgctaaa
     9421 cgagtcaagg cattgatatc attggttgcg tgtgccatca gatcacccgt ccgataggtt
     9481 tgataaaagg ctggcgacat ttttgtgaaa tgcttaaaca agcgagaccg catgatctgt
     9541 cccaagcaat aagaggtccc aaggatatac atacgccaca catagcgcaa atagtacata
     9601 ccaaaggctg caagtagcaa gtaaaatagg ctaagaagga ggtcctgctg ggttaattgc
     9661 cccgatgtga tggcatcaat gactcgcccc ataaccatag gaggaatgag attgagaacg
     9721 gaaaccaaga ccagggccac aatcccgact agataacggt gtttttctaa cttgaaaaac
     9781 caccagagtt tttgaataat ggacataaaa tccctttctg attgcaaatg gaaacctgag
     9841 gccaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaagc
     9901 actgctttga ggttgtagat agaactgacg aagtcagtaa cctacatacg gcaaggcgac
     9961 gttgacgcgg tttgaagaga ttttcgaaga gtacaagacc ccaggttttt cttattcata
    10021 agttacgact gtaacagcac ccttgtcata ttcagcaata aagatattgg ctacattgtc
    10081 atgcccttgt ttgctgaggt tatcaagcaa ccactcttcg ctacgaccaa tcgattccaa
    10141 gacgtctact tgaatcacac cgtcagtcac aactggatac ttaggatttt catctcccat
    10201 ttgcacaacg atgagttgcc cattttgctc ttgcacagct cgtttgactt gcttcatctg
    10261 gaaaatccct tggctacgaa gtttgagggc tacatccgat gcagacaaac caactgaacg
    10321 acaggcttat gggtcaatat tcccattttt gataaggaga gttggcttac catcaatcaa
    10381 acgtttcaca aagtgaacat tgttgttgag ctatttgaga gtcaagatca gtttgattaa
    10441 aatttcgata taattaagtg tcatttgttc acatccacca gttcaattgc gtctgtctta
    10501 tacaattcga ctttctctaa caggtacttg tctggctcac ttccgtttag ggcacgataa
    10561 tagcgatctc ccaccttgat aagtgcgcca tctgtgtttg tggaagtatt aacatggact
    10621 tctgacttgt ctactcccaa atctttggaa acaacctcga tgaaatgaag tgaagtttga
    10681 aatttattgt tagaaacttg attgttttga taagtcgtga tgctgaccaa aagcatagct
    10741 aaataaggcc agagctgaaa tcatgaccaa ctcacgaaat ttggttcctt ttttatcatg
    10801 gtatgccttg aaagcaaaaa aaactgtcac agctaataga acaatcccaa agccaaccat
    10861 gataccattt tgctgaccga tttggctgag tacatagtca taagagtaga atttcatgta
    10921 ttttcactcc ctttcataaa atcattctta attataaacg aaagagagta atttttcaaa
    10981 ccatacgttg gggaaataga tctttttttg gtataataaa atctataatc tgaatgaaaa
    11041 aggtaacttt atgaaactta tctcatggaa tattgattcc ctaaacgctg ccctaactag
    11101 tgactcagct cgtgccaaat tgtcccaaga agtcctacaa accttggtcg ctgaaaatgc
    11161 tgatatcatt gctatccaag aaaccaagct ttctgccaaa ggacctacaa agaaacacgt
    11221 ggaaatttta gaagaactct tcccaggcta cgaaaacacg tggcgctctt cccaagagcc
    11281 tgcccgtaaa ggctatgctg gaaccatgtt cctttataag aaagaactta cacccactat
    11341 cagcttccca gaaatcggtg ccccttctac catggacttg gaaggccgta tcatcactct
    11401 agaatttgat gcatttttcg taacccaagt ttacactcca aacgctggtg acggtctcaa
    11461 acgcttggaa gaacgccaag tctgggatgt caaatatgcg gagtatttgg ctgaactaga
    11521 caaagaaaaa ccagtccttg cgaccggtga ctacaacgta gcccacaatg aaatcgacct
    11581 tgcaaatcct gctagcaacc gccgttcacc tggatttact gacgaggaac gtgctggatt
    11641 taccaaccta ttggcaactg gatttactga taccttccgc catgttcatg gcgatgttcc
    11701 tgaacgctac acttggtggg cacaacgcag caaaacttct aaaatcaaca atacaggctg
    11761 gagaatcgac tactggctca caagtaaccg tatcgctgac aaggtcacta aatctgatat
    11821 gattgactca ggtgctcgcc aagaccatac accgattgtc ttggaaattg acttgtaagg
    11881 attttctata tggactacaa tgcggtcatt cccgagtttc ttgtatcgaa tatcgaacag
    11941 tcccggtcct tctactgtgg tttactgggt tttaggattg aataccaacg ttcagaagaa
    12001 aactttctct ttctcttgaa gagtgtcaac taatgttaga ggagggaaca aaggatcaac
    12061 tagctgaact gacctacccc tttggtcgtg gagtcaatct ctcctttggc ataaaggatg
    12121 ttccaaaact ctatcaaaaa gtgatggagg caaactatcc tatttatcgt cccttgacca
    12181 aaagaaagtt tcgtgtcagt gatccctata tctatcctca taaatttgca gttttggatc
    12241 cagatggcta ttttttaaga tttagcgagt agaataataa aaattgggaa aagtctttaa
    12301 aagttagaaa aaggcatatt gtcaggtgtc taaaaacttg atagtatgcc ttttatgatg
    12361 gaaaaatctt ctaatactct tcgaaaatct cttcaagcca cgtcaacgtc gccttaccgt
    12421 aggtatataa tactgactct gtcaattcta tccacaacct caaaacagtg ttttgaggtt
    12481 gtggatagct tcctagtttg ctctttgatt ttcatttagt ataaatgtta gcttacaatg
    12541 gagtttttgt tcaccctctc ttctgtttgt tcgtttttta aaccaaaaca ctaacaaaat
    12601 agttgatttc taaagttatt tagtgtaaaa taaaaacatt ggctcaagcc tatttaaaat
    12661 tgaatatcgt tttacttgtt tatgtcgtga attggcacga cgtttctaca aggtgccgga
    12721 acacctaaca ataagtaagt cagcagtgag atatggccgt ttttgccatg cctatttggg
    12781 ggacttactt agagattaag taggtctttt ttgaatattt ttaatatttc ccttaattag
    12841 tgcggacggg aggtgaaatt atcctgtgaa taatttcagg aatccaggga atttcatccc
    12901 taatttttga gcccctagct ctttcatttc taggcttagg ctaaaatagt tccccgaact
    12961 attttactct caaaaatccc gtcatagaca ataacaagat tattgtctat gataccacta
    13021 caacgggaaa tatttgataa gactcgcttc gctcgtaata gttttatcaa atatatagca
    13081 ttagcaaaag ggatcactat agaaacattt aaacaacaag taaattaact tttagacttt
    13141 aggaggtctt atgaaattat tagaagagcg catcctcaag gatgggcata tcttgggtga
    13201 taacatcctc aaggtagatt cctttttaac ccaccaagtt gactttagct tgatgcgaga
    13261 gattggtaag gtttttgcgg aaaaatttgc tgctgccggc attaccaagg tcgtaaccat
    13321 tgaagcgtcg ggtattgccc cagccgtttt tacagctgaa gccttaaacg ttcccatgat
    13381 tttcgccaaa aaagctaaga acatcaccat gaacgaaggc atcttaactg ctcaagtcta
    13441 ctcctttacc aagcaggtga ccagcaccgt ttctatcgct ggaaaattcc tctcaccaga
    13501 ggacaaggtt ttgattatcg acgatttcct tgctaatggc caagctgcta aaggcttgat
    13561 tcaaatcatc gaacaggccg gtgccacagt ccaagctatc ggtatcgtga ttgagaaatc
    13621 cttccaagat ggccgtgatt tgcttgaaaa agcaggctac cctgtcctat cacttgctcg
    13681 tttggatcgt tttgaaaatg gtcaggtcgt atttaaggag gcagatctct aatgcaaact
    13741 caagaaaaac actcgcaagc agccgttctt ggcttgcagc acttactagc catgtactca
    13801 ggatctatcc tggttcccat catgattgcg acagcccttg gctattcagc tgagcagttg
    13861 acctacctga tttctacaga tatcttcatg tgtggggtgg caaccttcct ccaactccaa
    13921 ctcaacaaat actttgggat tggactccca gtcgttcttg gagttgcatt ccagtcggtc
    13981 gctcccttga ttatgattgg gcaaagccat ggtagtggcg ctatgtttgg tgcccttatc
    14041 gcatctggga tttacgtggt tcttgtttca ggcatcttct caaaagtggc caatctcttc
    14101 ccctctatcg taacaggatc tgttattacc acgattggtt taaccttgat ccctgtcgct
    14161 attggaaata tgggaaataa cgttccagag ccaactggtc aaagtctctt gctttcagct
    14221 attactgttc tgattatcct cttgatcaac atctttacca aaggatttat caagtctatc
    14281 tctattttga ttggtctggt tgttggaact gccattgctg ctactatggg cttggtggac
    14341 ttctctcctg ttgcggtagc tccacttgtc catgtcccaa ctccactcta ctttgggatg
    14401 ccaacctttg aaatctcatc tattgtcatg atgtgtatca tcgcaacggt gtctatggtt
    14461 gagtcaactg gtgtttatct agccttgtct gatatcacaa aagatccaat cgacagcacg
    14521 cgccttcgca acggttaccg cgcagaaggt ttggccgtac ttctcggagg aatctttaac
    14581 accttccctt acaccggatt ttcacaaaac gttggtttgg ttaaattgtc aggcatcaaa
    14641 aaacgcctgc caatctacta cgcagctggt ttcctggttc tccttggact gcttcctaag
    14701 tttggtgccc ttgcccaaat cattccaagc tccgtcctcg gcggtgccat gctggtgatg
    14761 tttggttttg tatctattca agggatgcaa atcctagccc gagttgactt tgctaacaat
    14821 gaacacaact tccttatcgc agctgtttca atcgctgcag gtgtcggtct caacaacagt
    14881 aatctctttg tcagcatgcc gacagccttc caaatgttct tctcaaacgg aatcgtcgta
    14941 gccagcctac tcgccattgt cctcaatgcc gtattaaatc ataaaaagaa ataagaaaaa
    15001 gaggtgcgag cctctttttt gtttatcaca aattgattat aaatacaatc aatcccaatt
    15061 cccatctaat tgaattggac ctatttcaaa ttctttaaaa tattatgttc caaatcgtta
    15121 atattatata agaatggtaa aatatcaaat gtttctcgta aattattctt agccttatac
    15181 caaccttgtc catcagtaat ccacataaac tcaacattag gaatagcttt aatttcttca
    15241 gcaatcattt tgtaagatct agccgtctca tttaattttg aaccacttcc tgaataaaaa
    15301 ttaacctcaa tcagataaag cacttgttca ttttttatga caaaatcaaa acgtttcaca
    15361 gtattcccat cattcgtaat agcagataaa tcgacagaaa aaatttcctc tatttcattt
    15421 tgttcaatct ctttaaataa attctctcct aaaatatacc cttcagcttc aagataactt
    15481 tgaacaatat tttccatcgc atctccagtc cgatttttgc gcccattagt atccatacca
    15541 acctcaacac ctgtaacgta gtcaactaaa tttgatacta aatgattttg caataaatca
    15601 aatatacctg atttttccaa aaacatagta tattcttcaa tactatagtt tcgtttacta
    15661 aaatcaaaat agaaatcttt tatcggatcc ttaacaataa tcgtatctct caaacgttta
    15721 gcaataagta gaggcacaac tttcaaaatt tctggataat tttgatataa gtctaaaaaa
    15781 tcctcttgta tatttttact accaataaga ctattcataa tatttagagc aacttttata
    15841 ctagatacat ttttataaac cttgggaaaa tctgtataat aagtccagtc agctactgtg
    15901 tcggtcatcg ttgacaacca ctcatcaaaa tttctagttt gtttcatttt ctgcctccaa
    15961 cctcttcctt gcaattttta aatattcttt ttcagcatcg atacctataa atcttctacc
    16021 taaccgcttc gcaacaacac ccgtagtgcc actaccaaca aatgggtcta gaatatagtc
    16081 accctcttta gtagaggcta aaataatgcg ttctaacaaa tactctggtt tttgagttgg
    16141 atgtttccca gcccattttt caactttctt tgttaaagaa cccgtccaga catctttcat
    16201 ttgttttcca tcattcaatt ctttcattaa atcataattg tagtaatgac gagctttttt
    16261 atcatttttt ctggcccata aaatggtttc agtagaatgg gtaaaataac gacaagataa
    16321 attgggggca gggtttgttt tctgccaagt aatattattc agaattttaa aaccttcttg
    16381 ttctaatgcc attccaactg agtatatgtt gtgcaaacta cctgaaatcc ataccgtccc
    16441 attaggcttc agaacttctt ttgctaggcg aatccattta cgattaaact catgtttttc
    16501 ttcgaatgaa gaaattttat cccaatctcc tttatcaaca gaaacaactt gtcccccaga
    16561 attagatatt ccaccattac ttaaaaaata aggtggatca gcaaaaatca tatccatact
    16621 ttctggtttc atttttgata agaacttgaa cgtatctgaa tgaacaagaa tcatcttatt
    16681 tttattgtag tatggttttg tcataagaac acctccatac ttatattcgt tatttttcat
    16741 aatttgtgac tataatttca gaaatttttc ctcgacttga agattttgct ccattagttc
    16801 gggtagcttc aacataatgt atattaaaat ccttatacaa ctcctctact aaagcactag
    16861 aagaatttga taacataaca taagctcctg tatcactcaa tctcttaaag gcatctctta
    16921 atcttacttg atctgcaaaa gagaatccct catgagtata agacgtaaat gcactcgtct
    16981 cagacaatgg aatatatgga gggtcaaaat acacaaaatc tcctgttcga acatctacaa
    17041 ttgccttttc aaaatctccc actttaattt ctagttgatt gttatttaga taaactgaaa
    17101 tagcagatat caattcctca tcaacaattt taggattctt ataacgtcca tatggaacat
    17161 taaattggtt cttagaattc acacgatata gaccattaaa gttcactctc aacatatata
    17221 gaatacgtgc agctctttgt acttcggaca tcatatctat tctttcatca cgatctgcag
    17281 aacgtaaatc taaataatat tcttttgaat tatattcctg atgaactttc aaaatttcaa
    17341 tcaattcttg aggattgtcc ttaatttgtt gatagcaatt tattagttca gcgttaaaat
    17401 cattaataac tgcatcttta ggagccaaat caaaaaataa agctccacct ccaacaaaag
    17461 gttcgaaata cctgttatag gttttaggca ttaattctct aataacaggc aataattgtc
    17521 ttttaccacc tgtccatttc gtgaacggtt gtaaagtaac tttctttatt tcttttatct
    17581 tcatgctttt aattatacca tagaaattaa aacacaactc ttgtattttc taccacatta
    17641 ctaaattttc tattttagaa ttaaatcata tcaatataaa aaaatgagta aggcaaatct
    17701 tataaattcc aaaattcacc tcactctatt aaattactct ttatatcctt acccgtcttt
    17761 cctcagacct ttggcctgtg acaaacatag taaaggttgc tttgcagaca tttctgccct
    17821 cttgattggt gatatccaca tccatcacac aggttgtacg accttgatgg acacattctc
    17881 ctttaatggt caatacatcg tcgagttttc ctgccttaag gtagttgata gaggattgga
    17941 gtgtcacccc atcaagtccc agcgagataa ccaccaaacc actgatttgg tcacatagtg
    18001 tgaatagata gccaccatgg gcattgccat agtagttgag cgacgagttc actactttgg
    18061 tcgtcaccac aacgtgacca tctctcattt tttcaatttc gtaattttca aaggcagata
    18121 tagcgtcaaa atgaaagtct ttcatcgttt cctcctgata tttcaaatga cactatgata
    18181 ctacttttta taggactgtg caaggagaaa gctcctagtc tagtaaaatt ccgacatgtt
    18241 ccacaaagcg cataaaggct gtctgccctt gttctgtctg cttgtagact cctgcatcct
    18301 caagtacacg cgcaaagata gcacccacag agtccttgac gattgcaagg gctttttctt
    18361 tatccgttag gtctggatgt tgggatttga gttggtctgc ccactcctga tgataatcgg
    18421 caactgtaac agcttctcct acaagatagc tagcgacttg ctctacttct tctttcagac
    18481 gtggtggcaa gattgccaag cccatgacct caatcaagcc gatattttcc ttcttgatat
    18541 gttggacatc cttgtgggga tgatagatgc catcaggatg atctgctgaa gtctgattgt
    18601 ctcgcaagac caagtccaac tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg
    18661 tgtgatgcgg tgtcccgtct gtctctgcta aaatctgcac actagaatct gaatactgac
    18721 gccattcctg gaaaatctta tcagctaagt taatcaaatc ctctttggaa tccgaagtca
    18781 agcgtaggac agacattggc cacttgataa ttccagcctt gacctgctca aaaccagcaa
    18841 atcggaaggt cttttgcaag ggagccaatt ccataggaaa tacgtgacgg cctccctgat
    18901 aatgatcatg agttagaata gagcccccca caatcggcag gtcggcatta gatccagcaa
    18961 aatatcctgg aaactgctct acgatagcca acagacgttc aaaactctga cgactaatgg
    19021 ccatgggacg atgctggcca tctaaaaaga tacaatgctc attaaagtaa gcatagggcg
    19081 aatactggaa accccattcc tgaccaacca tttcaaaacg gataatccgg tggttgctgc
    19141 gagctgggtg gttgacccga ccatggtaac cctcattctc taaacaaagt tgacactgag
    19201 gataattact agcttgcacc aacttggcta ccgcaatctc tttcggatcc ttttcaggct
    19261 tagagagatt gatggtaatt tcaagttctc cataatcaga tggaacacga taaacgatat
    19321 ttttagcaat ggccttgagt ttgatgtagt catttttctg actgagttga taaaaatcct
    19381 ctatcgcttg ctcaggagag taggcatagg cttcccagaa gtcacgattg acctgactcg
    19441 gacaaggagt caccaagtcc atcaattcag taccaaggat ttcacgcgca gtctgactat
    19501 cctcaatcgt ctctaatcga acggcttcct caactagctg gtccttgagg tcaatcaatt
    19561 tatccagatt ggtctcaact tccaaaacac cttctcccac tcgtgccaaa acacgattgg
    19621 tcaggtagat tcgatccatt tcctcaaatg aactttcaga aatgacatgt gttacaaatt
    19681 tatctactaa ggtcactata gagcctcctt ttgactagtc aaggacgcga gtgccacctg
    19741 caacttcagc gatatagaag cttggagcgt atccaactac ttcctcgtag tgtttgccta
    19801 cagcttcctt aaaggcctca acagtatctt tttgaaccaa ggcaatggca cagccaccaa
    19861 aaccagcccc tgtcatacga gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa
    19921 gagtatccaa ttccaaacca gttacttcat aatcatgctc cagagaaacg tgtgacgcat
    19981 tcatcaaacg accaaatgtt tccaaatcac ctgcttgaag ggctgcttga gctttaaggg
    20041 tacgttggtt ttcaaacaca gcatggcgag cacgtttcaa acgattttca tctttaatca
    20101 gatagctata ttggtcaacg gcccactcgt ccaattcacc cagggtctga atatccaagg
    20161 caacttgcaa ttcttccact gctttttcac actcagcacg acgttcattg tatttagagt
    20221 ccgccaattc acggcgtttg ttggtgttca tgataacaac gacattgtcc ttcaaatcaa
    20281 gtggcaccaa gtcgtattct aaagtattgg tatctaagta aatagcacgt tggtcagccc
    20341 ccataccaat agcaaattgg tccatgatac cagagttgac tccgataaag ttattttctg
    20401 tttgttttcc gattttaacc aaatcgagac gctctagttt taaatcaaag agatgctctg
    20461 ccacgactcc tgtcaagagc tccaaggatg ctgaagaaga caaaccagca ccatttggga
    20521 tattcccata aacataaaaa tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga
    20581 aatgaaggac accttttgga taattggtcc agttgtgctc tttttcaaac ttgaggtcag
    20641 cgagaggcac ttcgataatg cccttgtcct caaagttagc tgagtagaaa cgcaagactt
    20701 ggtcgtcacg cttacgagct gcaccgtaag ttcccaagga aatagcagca ggaaaaacgt
    20761 gcccaccgtt gtagtctgtg tgttcaccaa tcaaattaat gcggcctggt gaaaagaagg
    20821 tttggtctgc ttcttgacca aaaacagcaa gaaagtcttt gcgaagggct tcagtagtaa
    20881 gatgttgtgc catatgaatt ctcctttgac tgtccgttaa gccaccttaa cgtataatcg
    20941 ttttctttct attgtatcca aggaattttc caagatcaat cctttttact aaaattttag
    21001 taaaatatca gtaaaaagaa gaaaaacatg atatactaac ctaatcaagg aggatttatg
    21061 gctaccttaa aagacattgc acagctagcc tctgtctcta tcgcgaccgt atcccgcgtc
    21121 ctcaatcgcg accagagcct atctgttaca gaagaaacca gacaccgtat tttaaccgtt
    21181 gctgaagagc tgggctacac caagcacctc aagacaggcg agtcccacaa acccaagcaa
    21241 aagattgcca ttatccaatg ggtcagcgaa caaggggagc tggacgacct ctactactac
    21301 cagattcgcc taggaataga aaaaagagcc caagagttgg actatgatat cttgcgctat
    21361 tttaatgacc atccttttac cttaagcgag gaagtgattg ggattctctg cattggaaaa
    21421 tttagccgag ctcagatttc tgcctttgaa gaataccaaa aacccctggt ctttctagac
    21481 agtgatacac tttctctggg gcatacttgt attattacgg atttttacac tgccatgaaa
    21541 caggttgtcg attatttcct cagccaagga atggaccgta tcgggattct aacaggcctt
    21601 gaagaaacaa ctgaccaaga agaaatcatt caggataagc ggctggaaaa tttcagaaac
    21661 tacagtcaag caaaaggaat ctatcatgat gaactggtct ttcaaggaaa ctttactgcc
    21721 cagtctggct atgacttgat gaaggaagcc attcagaact tgggagacca actcccacca
    21781 gcatttttcg cagccagcga tagtttagct atcggtgccc ttcgtgccct ccaagaagct
    21841 ggaatcagcc taccagaccg tgtcagcctc atttccttta acgacaccag cctgaccaag
    21901 caggtttatc ctcccctctc cagcatcaca gtttatactg aagaaatggg gcgagcaggt
    21961 atggatattc ttaacaagga agtcctccac gatcgcaaaa ttcctagcct gaccatgctg
    22021 ggaaccagac tgacattaag agaaagtacc ctaaatcaag aataggataa cataaaaaac
    22081 gaatagagtt ctaaaactcc tattcgtttt ttattcgatt acaatcatag acttaatggt
    22141 cttacgttca tccatatctt tgtaggcttg gtcgatatct tccagtttat aacttgaagt
    22201 aaagacgcga cctggattga tatcaccatc aaggacggct tttagtaaaa attgcttatc
    22261 gtatgttgta gcagaagctg ccccacctgc tacagagata ttttgcataa atgtcgaacc
    22321 aagagcacga ttattatagt gtgggactcc tacaaagccc atacgccctc cattatgaag
    22381 aacacctagc gcctgttcta tagcagcctc cgtaccaaca cattcaagtg ctgcgtctgc
    22441 tcctccaccg aggatttcac gcaccttggt aattccttct tgaccacgtt ctgcaacaac
    22501 agctgtcgca cctgactcca tagccatctt ttgacggtct tcatgacggc tcataaggat
    22561 aatttgtgat gctccacgca tcttagccgc gatgacagca cattgaccaa cagccccatc
    22621 accgataaca acaaccttgt cccctttttg aacatttgca acacgcgccg catgatagcc
    22681 tgtcggcatg acatctgcaa gagtcaaaag ggactggagc atcccttctg tatagtcaga
    22741 aggttgacca gggattttaa ccagcgccca gtttgcatag tggaagcgaa tatattctgc
    22801 ctgaaaatca ccccccaaat tattgccaat atgattgtcg caagaaccgt caaatccagc
    22861 aagacaggca tcacactcac cacatccatg tgtaaaaggg acaatcacaa aatcacctgg
    22921 tttcaccgtc gtaatggctt ccacagcttc ttcaacaatc ccaatcgctt cgtgtccact
    22981 atttttgtgt ccagctttcg tttctggatt acggtacctc cataaatctg aaccacaaac
    23041 gcacgcacga accacacgaa taatcacatc atccgcttct attatttgcg gacgttcaat
    23101 gctagcaagt ccaacctgac ctgcctttgt atatactgct gatttcattt aaaattttcc
    23161 ttccttataa agtttaattt tgagatttaa acgatttaaa gctgtctgta gctgagacaa
    23221 gcgctcctct aatttttgct tttcctcttc taaaatacca agcctctcct ctctcgtttc
    23281 atctcccttt tggtagagcg acatatagtc aactaaacta tctacagaga cacccgccga
    23341 acgaaaacac ttaataaatt ccagcgcttc gatatcctga tcttgaaaat cacgaatccc
    23401 agtagcagta cgagtaatcg gtggcacaag accaacccgt tcataatacc gaatcgtatc
    23461 cgctgaaatt cccaacaaat cactggcaga tttaatattc acacgctaac ctcctctcat
    23521 ttcattcact gaattcacgc tattctaact cacttatctt attatataac tttgagttga
    23581 ctccaagtca agcattttgt atctaatcga aaaattttta tcattaacaa tcatatatct
    23641 ggagtttata caatgctctt ctcttaccaa tttttgaaat atttaaataa taaagaaatt
    23701 actctattcc aaataagata gtacccaagt atttttcact tttttctaat gttgatgctc
    23761 ataactccgt tccaagtcac acacctttcg cttatgggtt tggtgagttt ctaggtcagc
    23821 atcaatttca atggtaatat tttgaaaacc acaatctttt aggaaaattc gaatagactc
    23881 tttacaagtt tccatatgtt ccatttcttt tagacaaaca tggacaatgg catttttttc
    23941 caaagcatcc atagtccaga gattaagctg attaaggctg gccacattgt ccaatcgctc
    24001 caggccactc tttacttgct tgatatcaag accttctggc acagcatcca agaaaatctt
    24061 gagtgtagac caaaaacgtg gaagggcttt tgaaagaata aagaaagaaa tgacaaggga
    24121 caaaagagga tctaggatat accagtccgt aaatcgaaga acaatcgcca tcaggataac
    24181 agctacccac cctagcgtat cttccagaaa atgcagactc agaatagact cattctttgt
    24241 ctttccctta ccaaccacca gactcgctaa cagattgata gtaatcgcaa taattcctaa
    24301 ccagagaatc ccctcatcat tgactggttg cggatgcaaa atcttcgtga cattttccaa
    24361 aatgactaga acagagcccg ttacgagaat cacagctgtt accaaggctc ctagcaggct
    24421 aaaccgctta tagcccaagg tgtactgatt gtcttcttca cgattggaga ttgtttctag
    24481 aaaagctgat attccaattg caatcgcatc tcccaagtca tgcacagagt cagcaagaac
    24541 agcgctagaa ccaaatactc cacctgcaat aaactcaaca atggcataag tcaaatttaa
    24601 gaaaaaagcc acccaaacag catattttgc cttcatattt ctcattcctt tgttataata
    24661 gatttatgaa caccttgttc attatcatta tccactaaaa ctcaaagaaa ggatagaagc
    24721 aaaacgtcag ctttattcag ttctgaacaa tttgccttaa gtgtccatat gactaacatt
    24781 gaccgccgta tcagcaaaac caaaaaagcc atctatcaag cttttataca acttttgaat
    24841 gctaagggct acgaggccac tactgttcag gatatcattg atctcgcaga tgtgggacga
    24901 tccacctttt actgtcacta tgagagtaag gagcttcttc tggaccagct ctgccgctac
    24961 ctcttccatc atctttttga aagagagcaa gccatttcaa ccgaggacta cctcgcccac
    25021 ctctttctcc attttcataa aaaccaagac catatcacca gtctgctatt ttccaaaaat
    25081 gactacttcc tccgtcaact ccataaagag ctagaacacc atgtctattc cgtgctagct
    25141 gataatttaa aagaagccca ccctaatctg cctacttctt acctccaaca cttggtcatg
    25201 tccaacttta tcgagacatt gacctggtgg ctcaaaaaag gtcaagattt cacagaccag
    25261 gaagttgtcc aattttatct agaccttctc attcctaaaa attgaatata gagtaaagct
    25321 tcagttgtct tatttctagg ttactgagtt ttttatcttt tcaacaacaa aagaggaccc
    25381 gccgatcctc tttttcatac tataaatcct tgattatcaa ctatatctgt tttaatcgaa
    25441 atctcaaaac agcactttca aacatctttt cctagttaag taaatcagta ttctgcttag
    25501 ctgccttgct ccattgatac caaccaacta gactgttaat gagataaatt agatatttcc
    25561 cttgaatttg caggctttct ccccaccaga gatagattga aaagacattg gtagccgccc
    25621 agaatatcca ctgttcacgg taaacagctg tcatgaggat ttgccctacc ccattggttg
    25681 catctgtgat tgaatcacga tagggacgat tggcaccaat agactgataa atgaagccaa
    25741 aggccaacca ccaaagcaca ctaatggaaa gatactttgt ccagcccttg ccgtccagtt
    25801 tacgcgcgac aaactcctgc ttttccttct taaactgtgc ctgataaatc caaactagaa
    25861 gtccaattgg ctgcatgact gtgaagtaaa gtgtcgtcag cacctcacca taaaagcctt
    25921 tctgtagggc caaaataagg taaataacag agttaatcaa gccaaaaaga taattacttg
    25981 ctcgaccttc cgatacaaag attacacaga taatccctgt caagctacaa atcatcccaa
    26041 tccagtcaac aatacgatgt tcgtaaacca actccagcca gagaggaaaa cttcctaaaa
    26101 ccagcaaata aatccactgg gcaaaactac gatgggcaaa gaggtcatcc cagatagcct
    26161 tcatagttcc tgaaaatcct aaatcagcca tagccgcaac catacgacgg taaccacctg
    26221 acatttcacc tagggttgtt ttgatatttt caattttctt ttgcaaataa gtatgcatca
    26281 tttctccttt tgtttttaaa gagccgtgtc tggatagact ttcggacgca acgctctatt
    26341 agataatgaa ctgcctatac acaagatttc taaccttagt cgacatgagc tgaaacctct
    26401 tatttgttaa gtagttcaca aaatattata cacctatttt atgaatagtc aactgtcttt
    26461 acagtaaaat tttagaaaat catgaaaatt ttctctttct ttccatttta agtgacattc
    26521 agtcattctc acatcaaaaa agcccagacg aaattgtctg agcattcttt tatctagtcg
    26581 tttaaggaag ttgagttcag tatgtttaaa gtctctgtcc catcatttct tcaacaaacc
    26641 ttgttcttgg agaaactcct tggctacttg ctttgctgac ttgccttcaa caccgacttg
    26701 gtagttgagc tggctcatct ggctttctgt aatcttacca gccaatgtat taagaactct
    26761 ttccaactct gggtgtttct tgagaagagc ttctttcatg agtggagccc cttgataagg
    26821 tgggaagagt tgcttgtcat cttccaagac ctgtaaatca taacgctcca attccgcatc
    26881 agtcgaatag gcatccgtga tttgaatatc ccctgactga atagcctgat agcgaagggc
    26941 tggctccatg gtcgctacat tgagattgag accatacatt gattgcaagc ccttatttcc
    27001 atcttcacgg tcgttaaact cgagtgtaaa acctgccttc aactgccctt ccactttttt
    27061 caagtctgaa atggtcttca agccatattc ttgagcaatc tttttcggaa cagctacagc
    27121 ataggtgttt tgataagaca tgggtttgag ataggctaga tgatcctgct tagcaatgcc
    27181 atcacgcgcc acctgataaa cctgttctgg ttcatgactc accttgggtg atggttgaag
    27241 caaactttca gtcaccgtac cagtaaattc aggatagatg tcaatatcgc cttttttcag
    27301 agcttcataa aggaagcttg tcttcccaaa attcggttta acagtcgcag tcatgctggt
    27361 attttcttca atcagcaact tatacatatt ggccaaaatt tctggttctg gacctatttt
    27421 cccagcaata accaagtttt ccttctcttt ttgaaccaaa agagctggac tataagacag
    27481 acccagtaat aaagccacca aggcaaaacc tgagaaaatc gtccgtaatt ttgctttttc
    27541 catcactttt agtaggaagt taaaggcaat ggctagcact gcagaagaaa gtgccccaat
    27601 caaaatcaaa ctggcattat tacggtcaat tcccaaaaga ataaaggaac ctagtccccc
    27661 tgcaccaatc aaggccgcca aggttgccgt accgataatc aaaacagctg ccgtccgaat
    27721 cccagacatg ataacaggca tggcgagtgg aatttcaaat ttcttgagac gttcccatct
    27781 ggtcatccca aaggcaatcc cagcctcttg caggttcgga tcaattccct tcagcccagt
    27841 gatagtattt tgcaaaatag ggaaaatcgc ataaatcact agagctgtca aagccggcaa
    27901 ggtcccaatt cccatcaaag ggataaagag ccccaacaag gccagagacg ggatggtctg
    27961 gaaaatacct gcaatctgca agacccagtc ggccagcttc tcatgatagc gaagaaaaac
    28021 agccaaggga atcgcaagca aaatagctag taacaaggtc aaaagcgaca actgcaaatg
    28081 ttgagataga gctgtcaacc aatcactaaa acgatcctga aaagttgcaa ttaaattagt
    28141 catgaacact acctccaaac aagtctgcta caaagtctgt tgcaggcgct tttaaaattg
    28201 tctcgggatt cgctacctgg cgaatttctc catcctgcaa gacagcaata cggtccgcca
    28261 acttcaaggc ttcatccgta tcatgggtta caaaaatcgt tgtcatccca aactctttat
    28321 gcaattcttt tgtcagaacc tgcaactgtt ttctcgaaat agcatccaag gccgaaaagg
    28381 gttcatccat gaggaaaatc ttgggctgac caatcatagc tcggacaata ccgacccgtt
    28441 gctgttctcc accagataat tcactaggta agcgatgccc atactcggct actggtaaac
    28501 caaccttagc caaaagctct tctgttttct tcgtaatttc ttccttgctc caccccttca
    28561 tttcaggaat gagagcaata ttttccgcaa ctgttagatt tggaaaaaga gcaatagcct
    28621 gtaaaacata accagtagaa agacgaagtt cacgctcatc atagtctttg atgcgcttcc
    28681 catccatata aatatttcca tcagttggtt ccaaaagacg gttaatcatc ttgagcatgg
    28741 tcgtcttacc tgacccagaa ggccctacta aaaccataaa ttccccatcc tcaatctgta
    28801 agttgacatc tctcaagaca tccttttctg tgtagcgcag tgctacattt ttgtattcaa
    28861 tcattctttg tcctcaattt aaaacttccc tcgattggtc aagtcttcta ccttaggcat
    28921 aacttcctta ttatcccaat gctccacaat tttcccgttc tctaaacgga agatatcgta
    28981 ctgggcataa gcaacgccat caatctgagt ctgaccatag ctaaccacat agtttccttg
    29041 tcctaagagt tggaaaacaa agtcaaaagt gacactatat tcagccacat agtttttata
    29101 agcagcactt ccttgtccaa tatcatgatt atgctgaatc aaatcgtctg ccacataatc
    29161 actccactgc tctagctccc cattttggaa aatttctgtc aagaaacggc gaaccagctt
    29221 tttattttct gctttcttgt ccaaatcctt gatttcaaaa tctccaaaaa tttgatctag
    29281 ttggtcattt tcaggtgttc gatagtagtc aacgacatcc caatgctcaa caatacgacc
    29341 attctcatcc gcacggaaag tatccgtcgt cacccattga gcttctccac cattcagata
    29401 ttgatgaaca tgaacaaaga ccagattgcc atcctcaatg gtgcggacaa tcttaatctg
    29461 acgctctgga tgacgctcaa agaaatctgc aaagaaggct gcaaatcctt ctttcccgtc
    29521 aggaacacct gtcgaatgtt ggatataggt atcccctaca gactgggctt gagcctcagc
    29581 aactcgcccg tcttgaatgg catggatgta taggttgtga gcatttttca cttgttgtga
    29641 catattctaa acctcatttc ccttctcttt cagattcgcc aaaattcttt cttgaaaacc
    29701 ttcaaattgg tgaatttctt cctctgaaaa tcctttgtaa aagatagtat ccaatttctg
    29761 actgacacga tgccccactt ctttctggaa cttgcctaac tccgttaaaa ctaaatactt
    29821 cttacgcttg tcttttccac acggactaac aattacaagc ttttgttcct ctagcttttt
    29881 tatcatagtc gtcagcgtat tattcgcaag cccagtcgca agcgcgatat ctgtcgcagt
    29941 tgcgcagcca gtttcactat tccataaaac cgctaaaatc ttgccctgtt cacccctata
    30001 aagagcctca ggatcttgac tcagtaactt ttgaaaaatc cgcccattca acaaacgaat
    30061 atgatgggct agcaaatgac tatctttcat aacacctcca atttatttcg atatcgaaat
    30121 gaataaaata attgtaacac tcatcgttct aactgtcaac tatttcgatt tggaaataat
    30181 ttttgataat tatccacacc accatactcc ggctcaacta acttttaacg agagtttcta
    30241 aactccttcg tcctccagtc tacaaaagcc ttccattcgt actatcctat attttatgag
    30301 gggacacatt tttcctatca gaccatttat tttaaagata gaagtaaatc ataattgctt
    30361 ccatctgttc ttttatagta tattgaagtt agactagagc actgtatctt ctaaaacatt
    30421 gatagaaagc gatttgaatt tcccaatcaa tttgttcgta tttatagcat tttgaaacta
    30481 gaataggaca ccatgactgc taaaagattt ctataaattc atttgatttt cctaatcaat
    30541 ttgttcatat cttatttcat tccgctataa tttcacctta ccctatcttt ttcgtagcac
    30601 ccttcaaaca gcctatcccc taccgtttga cgattcctca cttcgctcca cttccattac
    30661 agaagtttct tcactactat gggctcggct gacttctcat gattccttgt tactactatt
    30721 tgaacgctca cgagatagat cttacaaaaa atgctttgat ccacaatgga atcaaagcat
    30781 tttaaagagt tcctcataca taagcgcaga agtcgcagtt cctctgtact tggcttcttc
    30841 tcttttgata aagcgagcca agttgagcaa ctcaggtgct ggatgtttgg gatttaggag
    30901 caattcacga ttgaccaggc ctgagagacg aactgcctgc aattgctcat ttgtagtagg
    30961 cagtttttta gcagtctcta ggagagcagc aactaaatct tcactcaaat catgtcgagc
    31021 atgattgtaa agatctttta taaggctttc taggtttggt tctgccatcc ctaccacctc
    31081 ccttatggtt taataatgtt taatcaaatc aaccgttgaa cgatccaatt tcttcaccaa
    31141 ggcttgtaag aaagcttgcg cttctaggaa gtcatccatt gcatagaggg tttggtgaga
    31201 atggatataa cgagcgcaga caccgatagt tgttgatggg acaccaccat ttttcagatg
    31261 agctgcacct gcatctgttc cgcctttacc acagtagtat tggtacttga taccagcttc
    31321 ttcagccgtt gtcaaaagaa aatccttcat ccctgggaga agcaagtgac ctggatcata
    31381 gaaacgaatc aaggttccat ctccaatctt gccttgacca ccgtagacat cacctgctgg
    31441 tgagcaatca actgcgagga agacttctgg gtcaaacttg gttgtagagg tatgagcgcc
    31501 acgcagacca acttcttctt ggacgttaga acccagatag agttcattgc cgagtttttg
    31561 acctgataaa gcttcagcta gctcgcttac catgaggaca ccgtagcggt tatcccaagc
    31621 ttttgagatg atattttttt cattggctgt caaaattgca gaactatctg gtacgatggt
    31681 atcaccagga cggatgccaa aactttctgc ctcagccttg tccgcaaaac caccatcaaa
    31741 aacgatatcg gcaatggctg gcatggttgg tccccccttt ccacgagtca aatgcggagg
    31801 aacagaacct gaaatcacag gaatttcatg accatcacga gtcaagagtt tgaaacgttg
    31861 gctgctaacc accatggggt tccagccacc gatttctacg acacggaagg taccatctgg
    31921 cttgatttcg ctgaccataa aaccaacttc gtccatatga gaagcgacca agacgcgcgg
    31981 tgcatccaca gcttctgaat gtttgatacc aaaaatacca cccaagccat ctgtcaccac
    32041 ttcatccaca tgcggtgtca acttttcacg aagataagca cggacaggcg cttcatgacc
    32101 tgagactgca gcaagttctg ttacttcttt aatttttgaa aataatgttg tcatttcagt
    32161 tccttctttc tttcatccat tttaccactt tttataggag aaggatagtg ggaaggtgga
    32221 tttctaagtt agtatcttag tcctgctcta tcttagaaaa ggatagtatt ctcttgcatg
    32281 tagtgcaaaa tctagtaaac attccaaaat taactcgaat atttatttcc aaacaaaaaa
    32341 acaatacacc atcaaagttg tttgtatttt tcatgaaatt tacagaaaat agttgacttc
    32401 cctttcttct ttctttaaat atatagttgg ttgagtttgg aatagtacgc tgtagctgct
    32461 gaaacatttc tagaaattaa tttgactttc ctaatagagt tgttcatatc ttatttcaat
    32521 ttactatagt acaaaactag aaaaggaaaa aatcatgacc aaaacaattc ttgttacagg
    32581 tgggactagc tacattggct cccataccgt taaagctctt ttaaatgctg gctataaggt
    32641 gcatgtcctc gataatctct ccacaggaaa tcgagcggct gtggatagtc gtgctagctt
    32701 taaagaactg gatgtttatg atgctagcgc cttaaaggct tacttagaag aaaatcagat
    32761 tgatgctgtc ctccattgtg caggtgaaat tgttgtgagc gaaagtattg aaaatccaag
    32821 taaatacttc actgccaacg ttgctggtat gaaccaagtt ctcaaagtct tatctgaagt
    32881 tggcattcaa aaaatcatgt tctcttcgac tgcttctctc tatggaaata actgtattga
    32941 caagccagtg actgaagata ccctgctcga ccctgtcaac ccttatgcag agacaaaact
    33001 gatgggcgaa cgaatgattt actggatggc caatcgctac gactggaaat atgttatttt
    33061 ccgttacttt aatgttgctg gggctgaaat ggatgcttca aacggtctgc gtgtgaaaaa
    33121 tccaactcac atcattccaa atatcaacaa gaccgcattg ggacaaaatg atagcttaaa
    33181 aatatttgga gatgactacg atacacgtga cggttcatgt attcgagatt acatttatgt
    33241 cttggacctt gcataggctc atgttaaagg aatgaactac ctatttcaag aagacagttc
    33301 ttctcaaatc tttaacttag gaactaaaaa aggctatact atcaaagaaa tctttaaaac
    33361 tgctgaagaa ttactaaatt aaaaaatccc acacgagatt gttgctcgac gtgctagtga
    33421 cccagccagt gtcctagcag atgcttcaaa agctaaacaa tatcttgatt ggacggctag
    33481 ctactccctc gaagatatta ttttatcaga ttaaggtaaa aacattttag aataggataa
    33541 caaaggagaa agtcttgttg ctctctcctt gttgtattta ttaaattgtc agaaatttta
    33601 ttgacagatt agaaaaataa tgttacattt atatccgcag atatctttcg ataccaaatc
    33661 tacatgaaag gacggggtat gaaactttct cattatttaa ttggcttact tctactccta
    33721 gtctttctct ctattagcat tgggaccagt gatttttcat ggggaaagct atttgatttc
    33781 gaccagcaga catggctcct ctttcaagag tcccgtctcc caagaactat cagtattctc
    33841 ctgactgcct ctagtatgag tatggcaggc cttctcatgc agactattac ccaaaatcag
    33901 tttgctgcac cgagtacagt tggaacgact gaagccgcca aactgggaat ggtgctgagc
    33961 ctttttgtct ttccatcggc tagtctgacc caaaagatgc tcttcgcttt tgtttcatcc
    34021 atcgtattca ccctcttctt cctagccttt atgaccattt ttactgtaaa ggaaaggtgg
    34081 atgttgcctc tgattgggat catctatagc gggattatcg gctcagtcac agaagttatc
    34141 gcctatcgtt tcaatctggt tcagagtatg actgcctgga cccagggctc cttctccatg
    34201 attcagaccc atcagtatga gtggctcttc ttaggcctca tcatcctgat aaccgtttgg
    34261 aaattatccc aaaccttcac catcatgaat ctggggaaag aaatcagcga gagtttgggg
    34321 atttcctact ccctacttga aaaactggcc ctctttctgg tggcgctaac gacaagcgtc
    34381 accatgatta ccgtgggtgg cctaccattt ctcggagtta tcgttcccaa tcttgttcgc
    34441 aagcgctatg gagataatct aagtcaaacc aaactcatgg tcgcactggt tggtgccaat
    34501 ctagttctgg cttgcgatat cctatcccga gttctgatta ggccctatga gttgtctgtc
    34561 agtctcttgc taggaatcat cggtagtctc ggctttatcc tacttctctg gagaggggga
    34621 cgaaaagatg cagactaaaa gcaaacatac caagctcttc tggattctca ttattcttgc
    34681 catcggagct tgtcttctct acttttggcc catcactcac ttgtcagcct ttgcttggaa
    34741 gttgcgttcc caaaagatca tcgtttatct cttggtagcc atcgcgactg ggatttcgac
    34801 cattagtttt caaaccctga cggaaaatcg cttcctgacg cctagtattt tgggaattga
    34861 atccttctac gtcctactac aaaccctact actggttttt gaaagcaagt ttcttcaact
    34921 tggcaaatcc cctatcttag aattcctagt cttacttctt gtccagtccc tcttctttct
    34981 caccttacaa ggttacttga agacactgat gaagcaagac ctggtcttca tcctgctgat
    35041 ctgtctagcg ctcagaagtc tctttcgaaa tatcagcacc ttccttcaag tcctaatgga
    35101 tccaaacgaa tacgataaac tgcaaaatag tctttttgcc tcctttcaac atctcaacac
    35161 ttccatccta gccatcggtt ctctgatcat cctcgctttg acaatctttt tctttcgaaa
    35221 agcagtcgtt ctagatgtct tgcacctgca aagagaaacg gctcagatat tgggactcga
    35281 tgttgaaaaa gaacagaaag agctcctctg gggaatcgtg cttttgacct caacggccac
    35341 tgccttggta ggacctatgg ccttcttcgg ctttatgctg gccaacctca cctacctgat
    35401 tgtcaaagac tatcagcaca agttactctt tatagtggcc attctggttg gatttattag
    35461 cttaaccttg gggcaagcct tgattgaacg agtctttgca ctggaaattc gtatcagtat
    35521 gatcattgag agtgtgggtg gcttcttatt ctttatctta ctatatagga ggtctcgtca
    35581 gtgaaactgg aaaacattga caaatccatt caaaaacagg atattttgca aggcatttcg
    35641 cttgaagtca gtcctcaaaa actgacagcc tttattggtc caaatggtgc tggaaaatcg
    35701 actctcctct ccatcatgag cagactaacc aagaaagatc agggagttct cagtatcaaa
    35761 ggacgtgaaa tcgagagctg gaattcgcaa gaactggccc aagaactaac catcctaaaa
    35821 cagaaaatca attaccaagc caaattgact gttgaagaac tggtcagttt tggacgtttt
    35881 ccctacagcc gaggtcgact tagatcagaa gactgggaaa aaatccgaga aactctgaac
    35941 tatttggaac tgaccaactt aaaagaccgc tacatcaata gcctgtcagg ggggcaactc
    36001 cagcgcgtct ttatcgctat ggtactggcc caggatacgg actttatctt gctggacgaa
    36061 ccactcaaca atctcgatat caagcaaagc gtcagcatga tgcagattct tcgacgactg
    36121 gtggaggaac tcggcaagac cattatcatc gtcctccacg atatcaacat ggccagtcag
    36181 tatgcagatg aaattgtcgc cttcaaggac ggccaggtct ttagcaaggg aagcaccgat
    36241 caaatcatgc aggctgacct gctcagtcaa ctttatgaga ttcccatcac gctagctgat
    36301 atcaatgaca aaaagatctg tatctatagc tagtaacata aaagctcaag ttagagaacc
    36361 ttcagtctct tagtcaataa gattaagaga ctccctaaat cgttatcaca ttttaaaaag
    36421 gagaaattat gaaaacatcc cttaaacttt atttcactgc cctagtggcc agcttcttgc
    36481 tcctacttgg tgcatgtagt acaaactcaa gcactagtca gacagagacc agtagctctg
    36541 ctccaacaga ggtaaccatt aaaagttcac tggacgaggt caaactttcc aaagttcctg
    36601 aaaagattgt gacctttgac ctcggcgctg cggatactat tcgcgcttta ggatttgaaa
    36661 aaaatatcgt cggaatgcct acaaaaactg ttccgactta tctaaaagac ctagtgggaa
    36721 ctgtcaaaaa tgttggttct atgaaagaac ctgatttaga agctatcgcc gcccttgagc
    36781 ctgatttgat tatcgcttca ccacgtacac aaaaattcgt agacaaattc aaagaaatcg
    36841 ccccaaccgt tctcttccaa gcaagcaagg acgactactg gacttctacc aaggctaata
    36901 tcgaatcctt agcaagtgcc ttcggcgaaa ctggtacaca gaaagccaag gaagaattgg
    36961 ccaagctaga caagagcatc caagaagtcg ctactaaaaa tgaaagctct gacaaaaaag
    37021 cccttgcgat cctccttaat gaaggaaaaa tggcagcctt tggtgccaaa tctcgtttct
    37081 ctttcttgta ccaaaccttg aaattcaaac caactgatac aaaatttgaa gactcacgcc
    37141 acggacaaga agtcagcttt gaaagtgtca aagaaatcaa ccctgccatc ctctttgtca
    37201 tcaaccgtac ccttgccatc ggtggggaca actctagcaa cgacggtgtc ctagaaaatg
    37261 cccttatcgc tgaaacacct gctgctaaaa atggtaagat tatccaacta acaccagacc
    37321 tctggtatct aagcggaggc ggacttgaat caacaaaact catgattgaa gacatacaaa
    37381 aagctttgaa ataagccact tcaaactcaa tttaatactc aatgaaaatc aaagagcaaa
    37441 ctaggaaact agccgcaggt tgctcaaaac actgttttga ggttgcagat gaaagctgac
    37501 gcggtttgaa gggattttcg aagagtataa aattttgaca ttctaaaacg catcctatca
    37561 agttcactca aacctgatag gatgcgtttt tatcattttg aaatctattt accccacctc
    37621 attccccttc ttgatttcgt ttaagggaaa agtggtctgg aacggggtcc ttacctgttt
    37681 tcgaccaggg atgacaacgt aaaatccgag ccaagcccat caataccccc ttaaaaccat
    37741 gtttttcaat agcctgaatc atgtagttgg aacaagtcag ctcaaagcga caagagggtg
    37801 gaaagactgg tgagataaaa cgttggtaaa agcgcacagg cgctattaaa attcgtttca
    37861 ttatttctta gttacagcca tggtgtgtag ttggctgatt tcttttttat taagacgacg
    37921 ggattctcct ggacggagtc ctgtcaagtc taggtgtccg aaacgagtcc gagacaactt
    37981 atctacttgg agaccaacag cttcaaacat ctttttaacc tgatggttac gcccttcatg
    38041 gatggtcaac tgcaccacag agcgattttt gactgggtcc actttgagaa tttcataaac
    38101 agctggcttg gttttcttac catcaatctc aagaccacgg gtcaaggggc ggagattgtc
    38161 cttattggcc acacccttaa cacgcgcgac ataaaccttg tcaatctcat tacgagggtg
    38221 aatcatctcg tctgtaaagt ccccatcatt ggtcaaaatc aagacacctg atgtatccca
    38281 gtccaaacga cccacagggt aaatacgctc tttgacattg ggcaagaggt cgacaaccgt
    38341 cttgcgaccc ttatcatctg tcacactgga aatcacaccg cgtggtttgt taagcagata
    38401 gtagaccttt tcttcgttgt agataggttg accttcaact tcgaccttgt cgcctgactt
    38461 gatagtggtt gctagttcac gcaccacttg gccgttaacc gtcaccaagc cttgcttaat
    38521 cagctcttct gcttttctcc tactggccac acctgcgtgg gcaatatact tattgattct
    38581 catcttcttc tatcctttca ccaaataatt ggctttcttg ggcttgaatc tcaagctcat
    38641 caatcactgg taattcttct aaatggttta tccccatgta atctaggaaa taatccgtag
    38701 tcacatagag gttggggcgc cccaatactt cctttttccc gtcttccttt atcaggtcaa
    38761 aagcctgcaa ctttgccaag gctccactcg agttaactcc acggatggca tcaatttcta
    38821 tccgcgtaat cggctgtttg taggcaataa tggacaaggt ctcaagggca gcccgagaca
    38881 agctctggtt gataggcgcc ttagagtatt ccttcaaaat ctctgcaaat tgagacttgg
    38941 tcaccaatct ataagcacca cttgtctcaa tcaaagccaa actggaatct gggtcctttt
    39001 catacttctg ggctaatttt cctaaactct gctggatgcc tgtcggtggc agagagagga
    39061 gttcagctaa ctggcggacc cgaatcccat cttcacccgc tacaaacaag agcgcttcta
    39121 tttttgctaa agtactcatc tttcctctct atcaagtcta gctttgaggc acttgacttt
    39181 cttccttctt ttccatgaga tagatatctc caaaactctc ctcttgcacg aggatcagct
    39241 cctgggtttt gattaactct agggttgcca aaaagagggt gatgacctct tggacattct
    39301 gggcttcctt gaacaaatcc tgcaagcgca attgatctcg tccaatcaag gactctttca
    39361 cgataatcat catgtcctca atcttatact catcccgcaa gatagtcgtg tgattttgtg
    39421 caaactcctc ttttttcttg gctaggatat ttgaaaaagc caaaaagagg tcaatggtcg
    39481 tcttgtcatg cacaagctcc gcatcttcgt aaatcaactc tgtcggcgct ttggaataat
    39541 actgggcccg ttcttggtgc ttggcttcca agtgctcacc caagagcttg aacttgcgat
    39601 attcttcgat ttgagagagg aggtcctgct ccaggtcatc ccccaagtct gtcacttctg
    39661 ctaccttcgg aaggagttta cgactcttaa tcagcatgag ctgactagcc atgaccatgt
    39721 actcacccgt cacttccaga cgcatggcct gcagggttga aacataggct agatactgtt
    39781 cgatgacttc cgtaatgggc acatcgtaga tatccatctg gtacttagaa accagatgca
    39841 agagcaagtc cagggggcct tcaaaatctt ttaatttaat atccattatc tatatttttc
    39901 taaggtcagg actgttttta atcctaattt ttttgcaatt tcgtacaaat cgaccttgtt
    39961 ttctatttgt cttagaataa actgttcacg taagacttga gctgataagg atggaaaacc
    40021 tttctccttg acaaaagatt ctaactgacg aaaggcccac tgacgagaat agggtttctc
    40081 tcctctttca aaaagatagg tctgccccat caagggttcc aattctgaaa gcaaggccgt
    40141 gggaatggtg acaatcctct gttgggaagc cttgctgatt cgcaacacct gaaaatccag
    40201 attgatgtcc gcaaccttga tggctaaaat ctcactgggc aagagcccca tttctaggat
    40261 taagagcgct agcaagcggc cctctggatg gtcgctttcc tgccaaaaag agtctaggta
    40321 tagaatctct ggcttttccg tcttcttttc agcttgtttg gctaattcca agcggtaaaa
    40381 gctgtccacc tctccttttt gatagagaaa gtatagaaat tggttacagg ccgaaatctt
    40441 tcgcttctgg gcgctgattt ttagattggc tagctgggct tggtaaatct tgagactggt
    40501 ctcagaaatc cgctcaccta ccatgtctaa aaattgctcc aaatcatact tataggactg
    40561 cttggaattg acagacaagc cctgcttttc ctctaaaaag gctgaaatcc tgtctctcat
    40621 tggcatcgaa tctcatattc cttactaaag tcatgcaaga gggcattaac ggccttgagg
    40681 atggacttac gcgtaataat cccttggaaa ataccctctg catccacaac cggtaagaag
    40741 gactcatcta ctagcttgtg caagacctcc gtaatggtga aatcaggcga aacaaccgct
    40801 acgtccgttt ttgtcatatg aacgatatcc gtatccgcca tgatttcttg gctcaagtca
    40861 tgctccatct gataagccat aatatctctg agtccaatcg tcccaacaaa ctgtttttca
    40921 tctgtcacaa cgggaacacg ggtataggtc atctgactga gcaagagggt cgcatgatcc
    40981 gcattgtggg tatcaatcaa cacagctaga tttttagcag gggtcaaaaa agtttcctcc
    41041 tgccccaaca agaaagtctc aaactccttg gcaatcatcg gctaaactcc ttggacaaac
    41101 ctggatacac ctcgtgatct cgtgtcaaaa agtccacttt gaagtaacta tcatcaatct
    41161 ccacacgagc atagagacat tctctgatgg tacctcgtgg ttgactgata gaacctggat
    41221 ttagaaagag gatcttgcct tccaaccaag cacttggcac atgcaagtga ccatagaggc
    41281 agatagcggc ctcttcctcc tgagcccagt agtccaactt ttgaaagttg aaattgatgt
    41341 caaacaagtg accatgagtt tggataatct tggtcgaacc aagctcagtc accagacgtt
    41401 ctgggtagcc ggcgtagaag tccatgtttc ctttaacaac gcggatgccc tcccaaagtg
    41461 gagaatccgg acgtagttca gaatcgccgt tatgaaaaac ggcatcgact ttgcccacat
    41521 agcgatcacg gacttcttcc acaatcaagc tatcgccatg ggaatcgctc attacaatga
    41581 tggtttgctt tgccatgatg gaaatacctc caaaagtttc ttaacggcta aggcacggtg
    41641 agattgacta tttttttctt ccagggttaa ttcagctgat gacttacctg tctctcctac
    41701 aaggaagagg ggatcatagc caaagccatt ttcaccctta ggttcaaagt taatataacc
    41761 tgaccagtct gcttcaacaa ctaaactttc cttatttggg ctggctacga ctagggttgt
    41821 gtggaactga gccgagcggt ccttgagttc aaagaccatg gccaattcgt gcaagagttt
    41881 ggcattattt tcacggtcag ttgctcccac acctgcgaaa cgagctgacc agacgcctgg
    41941 taagccacca aggacatcga ctttgagacc agaatcatct gccaaaacca tcttgcccgt
    42001 taattgagaa atggtttctg ccttaaggcg ggcattttct tcaaaggtca tacctgtttc
    42061 tgctacttca ggcaggtcag ggtagtcatt aagattttcc acatcgtagc ctaacttatc
    42121 aaagatagct cggaattcct tggttttgcc ttcgttacga gtcgcaatca acaaggtttc
    42181 tctgaccttg cttgtctcaa agaaatcatg aacattgacc ccttctttag gcaattctac
    42241 atacaagagt tgcccatttt caaccaagag cagggcccca tgacgttgaa taacttccaa
    42301 gtttcgtccc atttcttgat taagcatgtc taccacaaag gagaacaaac ggtaggtaga
    42361 accatagcgt aaaacagtaa ctgaaagcgg gaaaccatac tcttcatctc caaatatttt
    42421 ggagaattca aacagaggaa aatctgtctt atagtcgctc aaactgttaa cgccaccaaa
    42481 aatactataa gacccaacat accaatcctg gtcatcctta tattcataaa ttttatttgt
    42541 cataattcta catgctccac ataaatctct ttttccagcc attcttcacc aatttgtgca
    42601 aaactttggc tactggctgt tgtgtaaaaa cggtgatgga gtggtccagc atcgcgacca
    42661 cgattgattt caaaataatt gagtaagact gagatatccc gtacgcactc tgccccacta
    42721 tcgatgagct gaacctttgg ccccatgaca ttttggataa tagggcgaag gagtggataa
    42781 tgagtacagc ccaaaatcag gctatccacc tttccaacca agggacgcag ggtttcatag
    42841 accaccttct tggtaacact ggttgacagg gcacctgact caaccaaggg agcaaacttg
    42901 ggacaggcca agctctccac ctgtaagtcg ggatccagat catggatttt ctgacggtat
    42961 atgtctgatt gtaccgtcat gggcgttcca atcactccga ttttcccacc ttgactggac
    43021 ttgatggctg ccgaagctcc tggcaaaatt acacccaaga caggaatatc tagttgagcc
    43081 ttgatttctt cccagacgac cgcagtcgca gtgttacaag caatgacaat cattttgaca
    43141 tccttggtca agagaaagtt gaccagctgc caagtatatt cacgaatttg ctcagctgga
    43201 cgggggccat agggcgcccg cgccgaatct ccaatataga cgatttcttc atggggaagc
    43261 tggcgcatga gctcgcgcac aacggtcaag cccccgacac ccgaatccaa aaaaccaatt
    43321 ggtcgattat ccatacagtc ttctttctag tcttttttct gattgatcgt tcattatgat
    43381 tccttgtact gaacgaactg accgacagct tgatagaatg tcaactacct ctctcttaat
    43441 cataatcaaa tatcaataga atgcaaggaa aaagtctcat ccctaaacca taaccaacat
    43501 agtgagaagt ctggaacttt catcccagac ttttccttat ttattttttc tttttattgt
    43561 tagcaagggc tgccttttgt tggcggatga tttggtggta aacttgttgt accttagctt
    43621 cgcttggttt ttgaccattt gcactcaaaa gagtacgaac tgcttcagca ttcaaacgtg
    43681 ggttgtcagc aaattctttt tcgatttgct tacgaaccaa gtacattcct ccaagagctc
    43741 ctcctagaaa agctagcaca atcaatacaa ttgctaaaag taaatccatc atttttctcc
    43801 tgtttctttt tgagtccctt tcattatacc acaaactagc caccctgacc agtttgttct
    43861 acactttcag ggatggaata gacagcaaaa agaaccctgc ttttctgaaa aagaggtctc
    43921 cttgctaagt ttaatttaca taaatagcca gtgtttgata gggttttagt agaattttct
    43981 cagacacttc tgcatcttca tagtttgaaa tcaaaatctg tccattttgg tagactgctg
    44041 gcaagtcgat ttccacttct ttagcataaa agttattgag cactagtaac ttttgatcct
    44101 caaactggcg ttcaaaagcg tagacttgtt tgctatcttc aaaggctggt ttgtaacttc
    44161 cttctgaaat gattggcatt tccttacgca gtcgaatcaa gtcttgatag aaggtaaaaa
    44221 tcggaccctg gatttcattt tctacattga tgtctttata ggatttacca gctttcaacc
    44281 aaggagtgcc tgttgaaaat cctgcatttt ccgaagcatc ccactgcatg ggaatgcgtg
    44341 aattatcacg cgacttagct tgaataatct ggaaggcttc ttgctgactc tttccttctt
    44401 ctaggagcat ctgataggca ttaagcgatt cgacatccac gtaatcagcc atagaatcat
    44461 agtctgggtc aatcatcccg atttcctcac ccatgtagat ataaggtgtc ccacgtgaca
    44521 ggtgaatgct ggctgctagc atggtggctc cttccttgcg gaagttttga atatcgacaa
    44581 aacggttcaa ggcacgtggt tgatcgtgat tgttccaaaa gagggcactc caaccgtctt
    44641 tatcactcat ttccttaccc caactatggt aaagactctt caactcttca aaatcaaagg
    44701 gagccaaggt ccacttttgt ccatccttat agtccacctt gaggtgatga aaattaaagg
    44761 tcatggataa ttcctgacga tcaggcgacg aatagaggac acagttttcc atggtggtag
    44821 aagacatttc cccaactgtc ataaagctat cgtcagatcc aaaagtggct tggttcatca
    44881 tacgcaaata gttatgaacg atgggtttgt ctgtataagc tggcttccct tcattttcag
    44941 gacagtccac tgaaacctcg tccttaccaa tcaaattgat cacatcaaat cgaaaacctt
    45001 tgacaccctt gtcgcgccag aaattaacaa ccttgaaaag ctccttacgg acattgggat
    45061 tgcgccagtt aaggtcagcc tgggtctcat caaataggtg aagatagtat ttcccagtat
    45121 ccccgaaagg cgcccatgca gaaccaccaa acttagactg ccaatctgtt ggttggtctt
    45181 ggatgaagaa aaagtcttga taatacttat caccagctag ggctttctga aaccattcat
    45241 gctctgtcga acaatgatta agtaccatgt ccagcataaa gtcaatcttg tgctctttac
    45301 cgatacacac catttcctca aaatcagcca tatcaccaaa aagaggatcc actgccatat
    45361 aatctgaaat atcgtaacca ttatcccgtt gaggacttgg atagaatgga ttgagccaga
    45421 ccatatccac acctagtttg gctagatagg gaatttttcc gataatccca cggaaatccc
    45481 caataccatt gccagtggtg tctttgtaag attttggata gatttgatag actacttttc
    45541 ctttatcaag tgtcatctgt ttctcctttt ctgataaaag ggaggaagca gtcttccgtc
    45601 cctatttgtg ctatttcaat tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
    45661 cacaggttgc tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga
    45721 ttttcgaaga gtattagatt cgtgtagcga ccatgagaga tgctccagct tgaatcgttg
    45781 tcggataagt tccgggaata gtcgctgtat aagcatcttg gttggtgatg ataacaggag
    45841 tttctgtcac cagacctgca gccttaatga catccatatc aaaacgaatc agttgctgac
    45901 caactgtaac gtgatctcct tggactacaa gactttcaaa acctttgcca tcaagaccta
    45961 ctgtatccat accgatgtgg atgagcaatt caactccctc gtcagagaca atgccgatgg
    46021 catgcttggt agggaaaaga accgtcactg tcccattaac tggagaggtc aactcacctt
    46081 ggcttggttc aatgactaga ccttgcccca tgacacctga tgcaaaaata ggatccgtcg
    46141 cttgactcaa ttctttcact tggccagtta gtgggctgat aatttctacc gaagtaagtt
    46201 ctactggttc atggttcaca aattctgctt cttcttgagc aacgaattct gcctgcaagt
    46261 tcgtatcgcc ctctgttttt gtaaagagac cagccttgcg gaagaagaaa gtcaagagca
    46321 ttggaacaac aatcgcaact agcatagttc ctgcaaatgg cagcatgtat tgaggttgaa
    46381 tagagagaat acctggcaaa ccaccgatac caatagaagc cgcagttaca ttaaaagtaa
    46441 cggataacat gcctgcaagg gctgaaccag tcatcccagc aacaaatgga taaatatatt
    46501 ttacgttaac cccaaaaaga gctggttctg taacaccgag ataggctgaa atggttgcag
    46561 gaagtgaaac ctgagcctca cgctcatcat ggcgatgcat gaaataatag gcaaacacgg
    46621 ctgagccttg agcaatatta gaaagagcaa tcattggcca tagggcagtg ccaccagcat
    46681 ccgcaatcaa ttgtgtatca atggcattgg tcatatggtg cagacctgtg atgacaaatg
    46741 gagcgtagag ggcaccaaaa attgcaccga agagccattt aactggacca gttaaacctg
    46801 ccaagacaac tgatgaaagt ccttgtccaa ttgtccaacc gattggtccc aaaacagtat
    46861 gagccaaaat caaggctgga atcaatgaca agaaaggtac aaaaatcata gaaatgactt
    46921 ctgggatatg cttgcgccag aagatttcaa gataagatag actcaaacct gcaagcaagg
    46981 ctgggataac ttgggcttgg taaccgatac gattaacagt aaaatagcca aaattccaaa
    47041 cccagtttgc cgcgatatca gctgctggcg ttgaagcaac cgcataggca ttgagcaact
    47101 gaggcgatac caaacagatt ccgagaacaa ttcccaaaat ttggctggtt cccatcttac
    47161 gagaaacaga ccaagtaatc cctactggta agaactggaa gatagcttca ccaggcaacc
    47221 agaggaaatg attaacacct gcccaaaact gagaggattc tgtgatggtc ttgccatcca
    47281 acatcgacca atggacacct tccaagacat tacggaaacc gaggatcaat cctccgacta
    47341 tcaaggctgg aataatcgga gtaaaaatct ccgccagagt ggtcatgaca cgttggacca
    47401 cgttttgatt actcttagct gcagacttgg ctgcttcttt ggaaacaccc tcaatacctg
    47461 aaacagctgt aaaatcatta taaaagatgg gcacgtcatt tccaatgatt acctgaaatt
    47521 gacctgcatt tgtaaaggtt cctttaacag ctggaattga ctcgatagct ttaacattag
    47581 ccttcttatc atttcctaaa acaaagcgca tccgtgttgc acagtgagtt acggcagtca
    47641 cattttcttt gcctccgatt gcctgaagca gatctttggc ttcttgttca aattttccca
    47701 tttgattctc cttatacttg tcaaagcaag tttgatttga taagtatatt gtaaacgata
    47761 ccaaacttgt ctgcaacttg tttggtataa aaagggtgtt tttttgtaaa ttttctagca
    47821 cttgtatggt aaaatagata caggtgttca ttaaactaga ctaaaaacct atttaagcag
    47881 gcaaaatgaa gaaataccaa caattattta agcaaatcca agaaaccatt caaaacgaga
    47941 cttacgctgt cggagatttc cttcctagcg agcacgacct tatggagcaa tatcaagtga
    48001 gtcgtgatac cgtccgaaag gccctgtctc tcctccaaga ggaaggattg atcaaaaaga
    48061 taagagggca aggttctcaa gtcgtcaaag aagaaaccgt caatttccct gtatccaacc
    48121 taaccagcta ccaagaacta gttaaagaac ttggactgcg ctctaaaacc aacgtggtca
    48181 gtctggacaa gattattatt gataaaaaat cctcactgat aaccggtttc ccagagtttc
    48241 ggatggtttg gaaggtggtc cgccagcgtg tggtggatga tctggtatcc gttctggata
    48301 cggactatct ggatatggaa ctcatcccaa atctcactcg ccaaattgct gagcagtcta
    48361 tctattctta tatagaaaat ggcctcaaac tccttattga ttatgctcag aaggaaatca
    48421 ccattgacca ctcaagcgac cgagacaaga ttctcatgga cattggcaaa gacccttatg
    48481 tcgtttcgat taaatcaaaa gtctatctcc aagacgggcg ccaatttcag tttaccgaaa
    48541 gtcgccataa gttagaaaaa tttagatttg tagattttgc aaaacgcaag aaataaaaga
    48601 ctgagacacc agatctcagc ctttttcttt atcccaaaac taattcatca ctgaccagac
    48661 tttggccact atttttctgg aagagatgca taaggtcttc aactgtcaga tgctttttct
    48721 cttctccctt gacatccacc actatcttgc cttgatagag cataatgaga cgattgccgt
    48781 actcaatcgc atgattcata tcatgggtaa tcatcaaagt cgtccactga tggtactcca
    48841 caatcttttg cgtcaagtcc atcaccattt gactggtttt cgggtcaagt gccgcagtat
    48901 gttcatccaa aagcaaaagt ttgagtttca ccagagctgc catgactagg gtcaaagcct
    48961 gtctttgtcc tcctgagaga tattgagtat ctacttttaa gcggttttca agaccaatat
    49021 tcaactcctt caaggcctct tggaactgga ttctatcctt ctccttcacg ccccaaccaa
    49081 gccctctctt ttgcccacgt ctcaaggcaa tagccatatt ctcctcaatc gtcaaacgag
    49141 aagctgttcc catcttagga tcttgaaaaa cacgggcaat atccttggct ctctttctga
    49201 cactcagatg tttaatggat ttccctgcca gtagaaggtc cccttcgtcc accgatagat
    49261 taccagccaa gatattcatc aaagtggatt ttcctgctcc atttccacca atcacagaga
    49321 taaaatctcc ctcttcaacc tctaaatcta atcctttgag gacatggttc tcattgaccg
    49381 tccctgcttc aaatgtcttg tgaatatttt caagtgttaa caaacttgcc ataactttct
    49441 cctcctattc atttctcaat ttcagaccac gaatctttaa tctcttttgc aatgctggtg
    49501 caaataaaac caaagcgagc aagattgcat taaagagacg taccaagttt tgatctagat
    49561 ttggaatttc atagatattt aaaataatca aacggtaaac aatagcaccg attccgatgg
    49621 ataataagcg gcctccaatg gtcaagtcgt gtatcaagac ttccgagctc aaaccaacaa
    49681 caatggttcc tgtcccagaa gtcacatctg aaaatccatc attttgggca aacaaggaac
    49741 cacatagggc aatcaaaccg gttgaaatca tgtaaccaac aatcttcatg gtgtttacat
    49801 tgactccatt ggcctcactc atcgggatat tgtccccagt cgaacgcaag accaaaccaa
    49861 tctctgtttt catcaaaagg gtcaaaacca aacaagtaag agacagacta aactaagtga
    49921 gaaaacagct ccttcatttg tcagacccaa gctcgccaac tgtttaaaga cggttgcaga
    49981 atctcccaag gaaagattgg gtacactgcc catgatttta atattgattg aatagagccc
    50041 tgtcaaggtc acaatccctg tcaagagagc tgggattttc atcttggtgt ggagcattcc
    50101 tgatataaga cctgctacca tacctgccag caaagcaagt aaagtcgcaa gccaaggatt
    50161 tgcccctgcc tgtatctgtg atacaacgat tgctgccccc ataggaaagg ctccctcagc
    50221 agtcatatcc gcaatatcca aaatacggaa agtcaagtag accccaattg ccataatcga
    50281 ccacaacaaa ccttctgata aactagataa cacaaaattc atcttaatcc tccttattcc
    50341 ttgccttcta acttactaat atcaattcac aatgcatctg ccatttcttg attagtatgc
    50401 aattccagtt tttcaggcaa ctcaactgct atattttctg gttcctcacc ttttaaaaca
    50461 cgaatcagca tgcgagctgt ctgtcttccc aattcttcat aattggttcc gtagttatac
    50521 aagccaccaa cagcaatcat ttctgttgaa ccaccgaata ctggaacctt gtgtttaata
    50581 gaaacctgct taactgtttc catggttgac gaaatgatat tatcagttgg gacaaaaacc
    50641 atatccacct ctgccatcaa actctctgct gccgccttga cgttattact gtccaagatt
    50701 gttttttcaa caacggtaaa gcctttttct ttcagaaatc gcttagcttc atccttttaa
    50761 acaaccgaat tgggctcgct ctgagtatag aggattccaa tcgttttagc ctttagcaac
    50821 actttcttta tcaaattgat ttgggttgaa atggcatctg atgactgatc gcttgtccca
    50881 gttacattgc ccccaggatg ttctcttgac tcaaccaact tggcactgac tgggtctgtt
    50941 acagctgaaa agataactgg tgtcgtttgc gtcgtattgg ccaagctctg agcagaaggc
    51001 cttgcgatgg ctagaacgac atcgctggat tctgctagtt gctgggaaat cgtttttaga
    51061 tttctttgtt ctccctgtgc attttgcaaa tcaatctcaa tattttcccc ctcaacatag
    51121 ccttgtttgg ccagctcatc cacaaatcct tctctagtag cgtctaagga ctggtgggta
    51181 ataaactgcg aaataccgat acgaaagaca tctcttttct tatctgcctt caactgatgc
    51241 aaactgatca gagaagtcaa gaggatcccc actaccaaga gggggctagt gattttcgaa
    51301 caactttcat aatgaaactc cttctcagaa aagataaaac aaaaaaacgc ctagatacta
    51361 tctaaacggg tacttgaaat aacctaacaa gttacaactt agctggtcca tttagatatt
    51421 catctatatg gaccacaatc aaaaatctta gccacataga tacaaacaaa ttgcttgaac
    51481 tgtttgcatc catggtgaca tgtctacaaa cactatctaa agtagctaaa gtaaaagttt
    51541 tgattcatag ttacggcttg tctcttattc atttcttttt ctgctttctt ttttgatcct
    51601 tcctatccta ccaccttttt catcacctgt caataatatt tcaaaatttt ttaaaatttt
    51661 tcgaaaattc tttcag
//
LOCUS       DAVBCB010000006        50102 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVBCB010000006 DAVBCB010000000
VERSION     DAVBCB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 50102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50102
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.006"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..746
                     /locus_tag="VQU28_000343"
     CDS             <1..746
                     /locus_tag="VQU28_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2475765.1"
                     /translation="YELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTD
                     KKFVFIIDEINRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVY
                     IIGTMNDIDRSVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAA
                     IENVQELNSHYHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLK
                     KAFELTNNEQKDQAVADDNESDENDDADY"
     gene            733..2085
                     /locus_tag="VQU28_000344"
     CDS             733..2085
                     /locus_tag="VQU28_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HET2475766.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKIIRGNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGALLKKWSIQIPQKSSSYSAFCKMMENSDYSAFCKMMENSEENFKAIIDEEVGRK
                     "
     gene            2260..3246
                     /locus_tag="VQU28_000345"
     CDS             2260..3246
                     /locus_tag="VQU28_000345"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HET2475767.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKQMHDQGLIASISVGVKDYEY
                     DFVSQLKTDAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            3310..3381
                     /locus_tag="VQU28_000346"
     tRNA            3310..3381
                     /locus_tag="VQU28_000346"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3343..3345,aa:Arg,seq:ccg)
     gene            3564..4262
                     /locus_tag="VQU28_000347"
     CDS             3564..4262
                     /locus_tag="VQU28_000347"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HET2475768.1"
                     /translation="MKELQTVLKNHFAIEFADKNLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            4253..7792
                     /gene="smc"
                     /locus_tag="VQU28_000348"
     CDS             4253..7792
                     /gene="smc"
                     /locus_tag="VQU28_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HET2475769.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKSEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRAGRATFLPLTTIKARTISSQNQDAITVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDAVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLRSEEAALKTLQDQMATLTERLEVIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQDRLAAIASDKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEELAQARLLKTELQGQKRYEVADIERLGKELDNLDFEQEEIQRLLQEKVDN
                     LEKVDTELLSQQAEEAKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRYLQSQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNIEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKDRFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            7789..8583
                     /locus_tag="VQU28_000349"
     CDS             7789..8583
                     /locus_tag="VQU28_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2475770.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFAGVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGETWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            8583..9401
                     /locus_tag="VQU28_000350"
     CDS             8583..9401
                     /locus_tag="VQU28_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2475771.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAASDRGIKVVLTT
                     GRPLKAMDFFLHELGTDGHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            9405..10625
                     /gene="ftsY"
                     /locus_tag="VQU28_000351"
     CDS             9405..10625
                     /gene="ftsY"
                     /locus_tag="VQU28_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HET2475772.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAVLEESQDSEPAVGVSQEEIEELPEEEKLEHEGTIEENNSEVLEAERPQTEETVQ
                     EKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEELLIMSDVGVQVASNLTEELR
                     YEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQDNLTVMLFVGVNGVGKTTS
                     IGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVDVPVVTGPEKADPASVVFDG
                     MERAVAEGIDILMIDTAGRLQNKDNLMTELEKIGRIIKRVVPEAPHETFLALDASTGQ
                     NALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREELNIPVKLIGFGEKIDDIGEF
                     NSENFMKGLLEGLI"
     gene            complement(10671..12158)
                     /locus_tag="VQU28_000352"
     CDS             complement(10671..12158)
                     /locus_tag="VQU28_000352"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HET2475773.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPAIFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRSVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVGASWKLIDRIEELWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDIAYRQDGRFE"
     gene            complement(12307..13047)
                     /locus_tag="VQU28_000353"
     CDS             complement(12307..13047)
                     /locus_tag="VQU28_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2475774.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDNLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(13047..15212)
                     /locus_tag="VQU28_000354"
     CDS             complement(13047..15212)
                     /locus_tag="VQU28_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HET2475775.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            15385..16338
                     /locus_tag="VQU28_000355"
     CDS             15385..16338
                     /locus_tag="VQU28_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2475776.1"
                     /translation="MKNKRIFKDFQASKMNLNLYTSPLLAFVFVFIGEFVAYTLYGIS
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENCLSNLLKGFSLGLALFLLTLLSLVVLGQYRLESIHLNPYYLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            16415..18403
                     /gene="uvrB"
                     /locus_tag="VQU28_000356"
     CDS             16415..18403
                     /gene="uvrB"
                     /locus_tag="VQU28_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HET2475777.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            18413..18976
                     /locus_tag="VQU28_000357"
     CDS             18413..18976
                     /locus_tag="VQU28_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2475778.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKTNDGYYQINGNS"
     gene            18942..19430
                     /locus_tag="VQU28_000358"
     CDS             18942..19430
                     /locus_tag="VQU28_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2475779.1"
                     /translation="MTVIIKSMETPEEIEGKPLVHWQTWREAYDGLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAMDGMKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LIKAALIALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDEQEKILELGKPIKEKRMVFY
                     SK"
     gene            19503..19970
                     /locus_tag="VQU28_000359"
     CDS             19503..19970
                     /locus_tag="VQU28_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2475780.1"
                     /translation="MSRAQLTILTNICLIEDLEAQRVVMQYRAPETNRWSGYAFPGGH
                     VENGESFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVVCYKATEFSGTLRS
                     SDEGEVSWVQKDQIPNLDLAYDMLPLMEMMEASDKSEFFYPRRTEDDWEKKIF"
     gene            complement(19967..20482)
                     /locus_tag="VQU28_000360"
     CDS             complement(19967..20482)
                     /locus_tag="VQU28_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HET2475781.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFKPLSELTDGVHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(20494..21021)
                     /locus_tag="VQU28_000361"
     CDS             complement(20494..21021)
                     /locus_tag="VQU28_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2475782.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGSLYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            complement(21299..21862)
                     /locus_tag="VQU28_000362"
     CDS             complement(21299..21862)
                     /locus_tag="VQU28_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2475783.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(21846..22397)
                     /gene="coaC"
                     /locus_tag="VQU28_000363"
     CDS             complement(21846..22397)
                     /gene="coaC"
                     /locus_tag="VQU28_000363"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HET2475784.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(22409..23098)
                     /gene="coaB"
                     /locus_tag="VQU28_000364"
     CDS             complement(22409..23098)
                     /gene="coaB"
                     /locus_tag="VQU28_000364"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HET2475785.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISADQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            23328..24998
                     /locus_tag="VQU28_000365"
     CDS             23328..24998
                     /locus_tag="VQU28_000365"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HET2475786.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELEIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNALSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            25326..26501
                     /gene="mutY"
                     /locus_tag="VQU28_000366"
     CDS             25326..26501
                     /gene="mutY"
                     /locus_tag="VQU28_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HET2475787.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     LIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            26556..27260
                     /locus_tag="VQU28_000367"
     CDS             26556..27260
                     /locus_tag="VQU28_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2475788.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            27253..28602
                     /gene="vicK"
                     /locus_tag="VQU28_000368"
     CDS             27253..28602
                     /gene="vicK"
                     /locus_tag="VQU28_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HET2475789.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            28604..29413
                     /locus_tag="VQU28_000369"
     CDS             28604..29413
                     /locus_tag="VQU28_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2475790.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            29504..29746
                     /locus_tag="VQU28_000370"
     CDS             29504..29746
                     /locus_tag="VQU28_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HET2475791.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            29748..30002
                     /locus_tag="VQU28_000371"
     CDS             29748..30002
                     /locus_tag="VQU28_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HET2475792.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            30049..30822
                     /locus_tag="VQU28_000372"
     CDS             30049..30822
                     /locus_tag="VQU28_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HET2475793.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     DSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLIEKT"
     gene            complement(31082..32068)
                     /locus_tag="VQU28_000373"
     CDS             complement(31082..32068)
                     /locus_tag="VQU28_000373"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HET2475794.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGLYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            32262..34730
                     /gene="gyrA"
                     /locus_tag="VQU28_000374"
     CDS             32262..34730
                     /gene="gyrA"
                     /locus_tag="VQU28_000374"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003244540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HET2475795.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIALRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEE"
     gene            34730..35473
                     /gene="srtA"
                     /locus_tag="VQU28_000375"
     CDS             34730..35473
                     /gene="srtA"
                     /locus_tag="VQU28_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HET2475796.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILTAFNQPYKQFY"
     gene            35717..36514
                     /locus_tag="VQU28_000376"
     CDS             35717..36514
                     /locus_tag="VQU28_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HET2475797.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            36662..36784
                     /locus_tag="VQU28_000377"
     CDS             36662..36784
                     /locus_tag="VQU28_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HET2475798.1"
                     /translation="MTCDFKFETLQLHAGQVVAPATKSRAVPIYQTTSFIFDDT"
     gene            36806..37846
                     /locus_tag="VQU28_000378"
     CDS             36806..37846
                     /locus_tag="VQU28_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HET2475799.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFAIPYLINVFSHGVDIAIHSATKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTCDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKDVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            38017..39291
                     /locus_tag="VQU28_000379"
     CDS             38017..39291
                     /locus_tag="VQU28_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HET2475800.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMKQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHEALLAKDKEV
                     QLLENQLATLRLEHENQLQKTLSDLEKERDQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKDFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            39301..40179
                     /gene="truB"
                     /locus_tag="VQU28_000380"
     CDS             39301..40179
                     /gene="truB"
                     /locus_tag="VQU28_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HET2475801.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAETPVLSPLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVTIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALTLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            40447..40722
                     /locus_tag="VQU28_000381"
     CDS             40447..40722
                     /locus_tag="VQU28_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HET2475802.1"
                     /translation="MEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQSVMANLSQGL
                     SLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            40830..41468
                     /gene="udk"
                     /locus_tag="VQU28_000382"
     CDS             40830..41468
                     /gene="udk"
                     /locus_tag="VQU28_000382"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HET2475803.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            41596..42936
                     /locus_tag="VQU28_000383"
     CDS             41596..42936
                     /locus_tag="VQU28_000383"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HET2475804.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            42914..43126
                     /locus_tag="VQU28_000384"
     CDS             42914..43126
                     /locus_tag="VQU28_000384"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HET2475805.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            43123..43998
                     /locus_tag="VQU28_000385"
     CDS             43123..43998
                     /locus_tag="VQU28_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HET2475806.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLESLQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            43991..44806
                     /locus_tag="VQU28_000386"
     CDS             43991..44806
                     /locus_tag="VQU28_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HET2475807.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHSVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            44799..45230
                     /locus_tag="VQU28_000387"
     CDS             44799..45230
                     /locus_tag="VQU28_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2475808.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            45237..46904
                     /gene="recN"
                     /locus_tag="VQU28_000388"
     CDS             45237..46904
                     /gene="recN"
                     /locus_tag="VQU28_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HET2475809.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRTIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLDLNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            46906..47634
                     /locus_tag="VQU28_000389"
     CDS             46906..47634
                     /locus_tag="VQU28_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HET2475810.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            47713..49536
                     /gene="lepA"
                     /locus_tag="VQU28_000390"
     CDS             47713..49536
                     /gene="lepA"
                     /locus_tag="VQU28_000390"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HET2475811.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(49738..49818)
                     /locus_tag="VQU28_000391"
                     /pseudo
     CDS             complement(49738..49818)
                     /locus_tag="VQU28_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
BASE COUNT        15242 a         8948 c        11370 g        14542 t
ORIGIN      
        1 tttacgagtt gtataaagat aaaaattata ataagcaaga atactacaaa agtggtggaa
       61 aaactgtcct agaaacattg agaaagagat ttggtttgaa agactatgtt tccccaacag
      121 aaattgatac tgataagaaa ttcgttttca tcatcgatga aatcaatcgt ggggagattt
      181 ctaagatttt tggcgaactc tttttctcta tcgaccctgg ctatcgtggt gaaaaaggaa
      241 gtgtttctac ccaatatgca aatctacacg aaactgatga aaagttctat atccccgaaa
      301 atgtttacat catcggaact atgaatgata ttgatcgttc agtggatacc tttgattttg
      361 ctatgcgtcg tcgttttcgt tttgttgaag ttactgccga gggtcaagtt ggcatgttgg
      421 ataaagagct gaatatccat gcagaagaag caaaaattcg tctaagaaac ttgaacgctg
      481 ctatcgaaaa tgttcaggaa ttaaacagtc attatcatat tggaccaagt tattttctta
      541 agttgaagga tgtagatttt gactatgaat tactctggtc cgattatatt aagcctctcc
      601 tagaagacta cttgcgaggt tcttatgatg aggttgaaac tttggaaact ttgaaaaaag
      661 catttgaact gacaaataat gagcaaaaag atcaggcagt agctgatgac aatgaaagcg
      721 atgaaaacga tgatgcggat tactgataat caacacaaga ttattaaaga aaaatttgtt
      781 gaagaatatc ctaaactaag caatcttctt ttagacagaa ccttggaaag tctatcccaa
      841 gatgaacgta ttttcatttt tccaaatgat ttgactcata ctccagattt ggataaaaaa
      901 caaaagattt tggaaacgat caatcaggaa atcaagacag gaaatgtgat tggttttctt
      961 ggatatggtc aggaaagatt aacgatttcc tcacgatttt ctgatgagag taatgaccat
     1021 tttttgcatt atctcttaaa caaggttctt catatcaatc tcactagttt agatgttgct
     1081 ttgtctcgtg aagagaggct ttatcaactt ttgatgtatc tcttccccaa gtatctacaa
     1141 gctgctattc gaaaaggtct ttataaggaa tatcatcgat tttctcataa cgacagtcat
     1201 gttaagggag tgattgatgt aagaaaccat ctcaagaaaa atcttccttt cacgggaaat
     1261 attgcctaca caacgagaga gttcacctat gataatcccc tcatgcagtt ggtccgtcac
     1321 actattgaat acattaagaa tcagaaaagc attggtcaag gggtactaga taatctctca
     1381 actagtcgtg aaaacgtatc tgaaatcgtg cgtgtaacgc cctcttataa actagctgat
     1441 cgtgctaaga ttattcgggg aaatcaatct aaacctatac gtcatgcata ctttcacgag
     1501 tacagaaact tacaagaact ttgtctgatg atcctaaacc aagaaaagca cggtttaggg
     1561 tatcaagatc aaaaaatcta tggtattctc tttgatgttg cctggctttg ggaagagtat
     1621 gtttacacct tgttgccaaa aggttttgta catcccagaa ataaggataa gacggatgga
     1681 atttcagtat tttctgttgg gaaacgaaaa gtatatccag atttttatga cagagaacga
     1741 aagattgttc tagatgcaaa atataaaaaa ctggaattga ctgaaaaagg aatcaatcgt
     1801 gaggacttat tccagctgat ttcctattct tatattttaa aagctgagaa ggctggactt
     1861 gtttttccga gcaaggacaa ggttattgat aatgagatgg gaaacctagc aggttatgga
     1921 gctttgttga agaaatggtc tatccaaatt cctcagaagt cttcatctta tagtgcattt
     1981 tgtaaaatga tggaaaattc agattatagt gcattttgta aaatgatgga aaattcagaa
     2041 gaaaatttta aagcgattat tgatgaagaa gtggggagaa aataatttcc cctctttctt
     2101 ttatttttaa cacaagtaaa acccgaacaa tatatttcaa ttcataaaat aatttgacaa
     2161 aaactgtact ttggtttata ataaattaag gaaacgtcgg aaaagacgta gtgatgcgag
     2221 agcataggta ggtcattaca aaagaaacga gacatcgata tgttaaatga atttccaatt
     2281 tttgattacg aagatattca attgattcca aataaatgtg tcattaaaag ccgtgcagaa
     2341 gcggatacaa gtgtcacttt aggaaatcac acctttaaac tacctgttgt gccagcgaat
     2401 atgcagacga ttttggatga aaatgtagca gagcaactgg ctaaaggtgg ttacttctac
     2461 attatgcacc gttttgatga ggcaggacgc attcctttta ttaaacaaat gcacgatcaa
     2521 gggctcattg cttccatctc tgtcggtgtt aaggactatg agtatgattt tgttagccag
     2581 ctcaagactg atgctcctga gtatatcact attgatattg ctcatggtca tgcggatagc
     2641 gtgatttcta tgattcaaca tatcaagaaa gaattgccag atacctttgt cattgctgga
     2701 aatgtgggaa caccagaagc tgtgcgtgaa ttggaaaatg ctggtgcgga tgctactaag
     2761 gtcggaatcg gtcccggtaa ggtttgtatc accaaggtta agactggttt tggtacaggt
     2821 ggttggcagt tggctgctct acgctggtgt gccaaggctg cgcgtaaacc gattatcgct
     2881 gatggaggaa ttcgtactca cggtgatatt gctaagtcta tccgcttcgg tgctagcatg
     2941 atcatgattg gttccctctt tgcaggacac attgaaagtc cagggaaaac gattgaagtc
     3001 gatggtgaac agttcaaaga atattatggt tcagcctcac aatatcaaaa aggtgcttac
     3061 aaaaatgtgg aaggcaaacg tatcttgctt cctgctaaag gtcatctgca agacacttta
     3121 actgagatgg aacaagacct tcaaagtgct atttcttatg caggtggacg tcaggttgct
     3181 gaccttaaac acgttgatta tgtgatcgtg aaaaactcca tctggaatgg ggatgcttcc
     3241 cactaagacg gggtctttga ccaaaaaact tgttattaga gcaaatttct gttataataa
     3301 aacaagtttc cacccttagt gtaatggata tcacgtaaga ttccggttct tgagatgggg
     3361 gttcgattcc ctcagggtgg atgtaaacat cctaaaaagc ctttaatagg gcttttttct
     3421 ttatcctgac ccaaatttgc cgctaaaact gagaatctct gtctaaagaa ttcccaaatg
     3481 ctttccagtc ccgttttaga gtgactcagg gggctttttt tgatataata aaaaggactg
     3541 ttatcagtta gaaagaggtt ggtatgaaag aattacaaac tgtactaaag aatcattttg
     3601 caatcgaatt tgcagacaaa aatttactgg aaactgcctt tactcatacg agttatgcca
     3661 atgagcaccg cctcttaaaa atttcacaca atgaacgctt ggaattttta ggagacgctg
     3721 ttctacagtt attgatttca gaatatctat ataaaaaata tcctaaaaag cctgaaggtg
     3781 acctatcaaa actccgtgct atgattgtcc gtgaggagag tttagctggt tttgcgcgtg
     3841 attgccagtt tgaccagttt atcaagttgg gtaaagggga agaaaaatca ggtggtcgca
     3901 atcgtgacac cattcttggt gatgcctttg aagcctttct tggtgccctt cttttggata
     3961 aggatgtggc caaggtcaag gaatttatct atcaagtcat gattcctaag gttgaagcag
     4021 gcgaatttga gatgattaca gactataaaa cccatctcca agagttactt caggtcaatg
     4081 gtgatgtggc tattcgttat caggtgattt ctgaaacagg gcctgctcac gataaggtct
     4141 ttgatgtaga agttcttgtt gaaggtaaga gcatcggtca aggccaaggt cgttctaaga
     4201 aattagcaga gcaggaagct gccaaaaatg ccgttgagaa ggggctggat tcatgtattt
     4261 aaaggaaatc gaaattcagg ggttcaagtc ttttgctgat aagaccaagg tcgtttttga
     4321 ccaaggtgtg acggcagttg ttggacccaa tggatctgga aagtccaata ttacagaaag
     4381 tctgcgttgg gctttggggg agtctagtgt caagagtctc cgtgggggca agatgccgga
     4441 tgtcatcttt gctggaacag aaagtcgcaa accgctcaat tatgcttctg tagttgtgac
     4501 tctggataat catgacggat ttatcaagga tgcaggtcaa gaaatcaggg tggaacgcca
     4561 tatctatcgt agtggagata gcgaatacaa gattgacggc aagaaagtcc gtctgcgtga
     4621 tattcatgac cttttcttgg atacaggttt gggacgagat tccttctcta ttatttccca
     4681 agggaaggtt gaggagattt ttaattccaa gtctgaggaa cgacgagcta tttttgaaga
     4741 agctgctgga gttttaaaat acaagactcg cagaaaagaa accgagagta aactgcagca
     4801 aactcaggat aatctggacc gcttagagga cattatctac gagttggata atcaaatcaa
     4861 gcctcttgag aagcaagctg agaatgcccg taaatttcta gacttagaag gtcaacgcaa
     4921 ggctatttac ttggatgtat tggttgctca aatcaaggaa aataaggcag aactagagtc
     4981 gacagaagaa gagttggctc aggttcaaga actcttgatg agttattacc aaaagcgtga
     5041 aaaattagaa gaagaaaatc aaactcttaa aaagcaacgc caagatttac aggctgaaat
     5101 ggccaaagac caaggcagtt tgatggactt gactagtctg attagtgatt tagaaagaaa
     5161 attagcccta tcgaaactgg agtctgagca agtggccttg aatcaacagg aggcacaagc
     5221 tcgtttggct gctttggagg ataagagaaa ttcactcagc aaagaaaagt ctgataaaga
     5281 aagctcttta gctctgttag agggaaatct agtccaaaat aatcaaaaac tcaatcgttt
     5341 agaagctgaa ttgctggctt tctcagacga tcctgatcag atgattgagc tcttacgtga
     5401 acgctttgta gctcttttac aagaagaagc ggatgtctca aaccagttga cccgtattga
     5461 gaatgagttg gaaaatagtc gtcagctttc tcaaaaacaa gcagatcaac tagaaaagct
     5521 gaaagagcaa ttagctatag ctaaagagaa ggctagtcag caaaaggaag agcttgaaac
     5581 tgccaaggag caggttcaga aattattggc tgactaccaa gctattgcca aggagcaaga
     5641 ggagcagaaa acttcctatc aagctcaaca aagccaactc tttgaccgtc tggataatct
     5701 caaaaacaag caggccagag ctcaaagttt ggaaaatatc ctgagaaatc atagtaactt
     5761 ttatgcaggt gttaagagtg ttctccaaga aaaagatcgc ctaggtggga ttattggtgc
     5821 agtcagtgag catctgacct ttgatatgca ttatcaaact gccctagaaa ttgcgcttgg
     5881 agcgagtagc caacatatca tcgtagaaga tgaagagtcg gcaaccaagg ctattgactt
     5941 ccttaaacgg aacagagccg gtcgtgcaac ctttcttcct ttgaccacta tcaaggcgcg
     6001 tacgatttct agtcagaacc aagatgctat cactgtaagc ccaggtttcc ttgggatggc
     6061 agatgagttg gtgacttttg atactagact ggaagccatt ttcaagaact tgctagctac
     6121 gacggctatc tttgatgccg tagaacatgc gcgtgcagca gctcgccaag ttcgttatca
     6181 ggttcgtatg gtgacattgg atgggacaga attacgcacg ggtggttcct atgcgggtgg
     6241 tgccaatcgc caaaataaca gtattttcat caagccagaa ctggagcaat tacaaaaaga
     6301 aattgctgca gatgaagcaa gcttgcgttc agaagaagcg gctttgaaga ccttacaaga
     6361 ccagatggct acattgacag aaagattaga agtcatcaaa tctcaaggag aacaggcacg
     6421 tattcaggag caaggcttgt ccctcgctta tcagcaaact agtcagcaag ttgaagaact
     6481 ggaaactctt tggaaactcc aagaagagga aatagatcgt ctttccgagg gagattggca
     6541 agcggataag gaaaaatgcc aagatcgtct tgctgcaatc gccagtgaca agcaaaatct
     6601 ggaagctgag attgaagaga ttaagtctaa caaaaatgcc atccaagaac gctatcaaaa
     6661 cttgcaggaa gagctagcgc aagctcgttt gcttaagaca gaactgcaag ggcaaaaacg
     6721 ttatgaagtt gctgatattg aacgcttagg caaggaattg gacaatcttg attttgaaca
     6781 agaggaaatc cagcgccttc ttcaagaaaa ggttgataat cttgagaagg ttgatacaga
     6841 attgctcagt caacaggcgg aagaagccaa aactcagaaa acaaatctcc aacaaggttt
     6901 gattcgcaag cagtttgagt tggatgatat tgaaggtcaa ctggatgata ttgctagtca
     6961 tttggatcag gctcgccaac agaatgaaga gtggattcgc aagcaaacac gtgctgaagc
     7021 caagaaagaa aaggtcagcg agcgcttgcg ctatttacaa agtcaattaa cagaccaata
     7081 tcagattagc tatactgaag ctctagaaaa ggcgcatgaa ttggaaaacc tcaatctggc
     7141 agagcaagag gtgcaggatt tagagaaagc tattcgctca ctggggcctg tcaatataga
     7201 agctattgac cagtacgaag aagttcacaa tcgtctggat ttcctaaata gccaacgaga
     7261 tgatattttg tcagcgaaaa atctgctcct tgagaccatt acagagatga atgatgaggt
     7321 caaagatcgc tttaaatcaa cctttgaggc cattcgtgaa tcctttaaag tgaccttcaa
     7381 acagatgttt ggaggaggtc aggcagactt aatcttgact gagggagact tgttgacagc
     7441 tggggttgag atttctgttc aacctccagg taagaaaatc cagtcgctta acctcatgag
     7501 tggtggtgaa aaagccttat cagctcttgc tttgcttttt tctatcattc gagttaagac
     7561 tatcccattt gtcatcttgg atgaggtaga ggccgcgctg gacgaagcca atgttaaacg
     7621 ttttggggat tacctcaatc gatttgacaa ggacagccag tttatcgtcg taacccaccg
     7681 taagggaacc atggcagcgg ctgattctat ctatggagtg accatgcaag aatcgggggt
     7741 ctcaaaaatt gtttcggtta agttaaaaga tttagaaagt attgaaggat gacaattaaa
     7801 ctagtagcaa cggatatgga cggaaccttc ctagatggga atggacgctt tgatatggat
     7861 cgtctcaagt ctctcttggc ttcctacaag gaaaaaggga tttactttgc ggtggcttcg
     7921 ggtcggggat ttctgtctct ggaaaaatta tttgctggtg ttcgtgatga cattattttc
     7981 atcgcggaaa atggcagttt ggtagagtat caaggtcagg acttgtatga agcgactatg
     8041 tctcgtgact tttatctggc aacttttgaa aagctgaaaa cgtcacctta tgtagatatc
     8101 aataaactgc tcttgacggg taagaagggt tcatatgttc tagatacggt tgatgagacc
     8161 tatttgaaag tgagtcagca ttataatgaa aatatccaaa aagtagcgag tttggaagat
     8221 atcacagatg acattttcaa atttacaacc aacttcacag aagaaacgct ggaagctgga
     8281 gaaacttggg taaacgataa tgtccctggt gtcaaggcta tgacaactgg ctttgaatct
     8341 attgatattg ttctggacta tgtcgataag ggagtggcta ttgttgaatt agctaaaaaa
     8401 cttggtatca caatggatca ggtcatggct tttggagaca atcttaatga cttacatatg
     8461 atgcaggttg tgggacatcc tgtagctcct gaaaatgcac gacctgaaat tttagaatta
     8521 gcaaagactg tgattggtca ccataaggac cagtcggtta tagcttatat ggagggctta
     8581 taatggcaga tataaaatta atcgcattgg acttggatgg taccttgctg actactgata
     8641 aaaggctgac ggatcgcacc aaggcaactt tgaaagctgc gagtgatcgt ggtatcaagg
     8701 tcgtattgac aactggtcgt cccttaaaag ccatggactt ctttctccat gagttaggga
     8761 ctgacggcca tgaagatgag tataccatta cttttaatgg tggattagtt cagaaaaata
     8821 caggagaaat ccttgataaa acagtctttt catatgatga tgtggcacgt ttgtatgagg
     8881 aaacagagaa attatcactg cctcttgatg ccatctcaga aggaacagtt tatcaaatcc
     8941 aatcggacca agaaagtctt tatgccaaat tcaatccagc tttgactttt gttccagttg
     9001 actttgaaga cctgtctagt caaatgacct acaacaaatg cgtgactgcc tttgctcaag
     9061 aacccttgga tgcagccatt cagaagattt ctccagaatt gtttgaccaa tatgaaatct
     9121 ttaaatcacg tgaaatgttg ctagaatggt caccaaagaa tgttcataaa gcaacaggtt
     9181 tggcaaaact aattagccat cttggaattg atcaaagtca agtgatggcc tgtggtgacg
     9241 aggccaatga cctctctatg attgaatggg cagggctcgg tgttgccatg caaaacgctg
     9301 ttcctgaagt aaaggcagcc gcaaatgtag tgacgccgat gaccaacgat gaggaagctg
     9361 tcgcctgggc tatcgaagaa tatgtgctaa aggagaacta agatatggga ttatttgacc
     9421 gtctattcgg aaaaaaagaa gaacctaaaa tcgaagaagt tgtaaaagaa gctctggaaa
     9481 atcttgattt gtctgaagat gttgatccta ccttcacaga agttgaagaa gtttctcaag
     9541 aagaagctgt gcttgaagaa agtcaggatt cagagccagc tgtaggggtt tctcaagaag
     9601 aaatagaaga attaccagag gaagagaagc ttgagcacga aggaactata gaagaaaata
     9661 attctgaagt tcttgaagca gaaaggcctc aaacagaaga aactgttcag gaaaaatatg
     9721 accgcagtct taagaaaact cgcacaggtt tcggtgcccg cttgaatgcc ttctttgcta
     9781 acttccgctc tgttgacgaa gaatttttcg aggaactgga agaactgttg attatgagtg
     9841 atgttggtgt ccaagtcgct tctaacttaa cggaggaact acgttacgaa gccaagcttg
     9901 aaaatgccaa gaaacctgat gcacttcgtc gtgtcatcat tgagaaattg gttgagcttt
     9961 atgaaaagga tggtagctac gatgaaagca tccacttcca agataacttg acagttatgc
    10021 tctttgttgg tgtgaatggt gttgggaaaa caacttctat cggaaaacta gcccaccgct
    10081 acaaacgagc tggtaagaag gtcatgctgg ttgcagcaga taccttccgt gcgggtgcag
    10141 tagctcagct agctgaatgg ggccgacgag tagatgttcc agtagtaact ggacctgaaa
    10201 aagctgatcc agccagcgtg gtctttgatg gtatggaacg tgccgtggct gaaggtatcg
    10261 atattctcat gattgatact gctggtcgtc tgcaaaataa ggataacctt atgactgagt
    10321 tggaaaagat tggtcgtatt atcaaacgtg ttgtgccaga agcaccacat gaaaccttct
    10381 tggcacttga tgcatcaaca ggtcaaaatg ccctagtaca ggccaaagaa ttttcgaaaa
    10441 tcacaccttt aacgggaatt gttttgacta agattgatgg aactgctcga ggaggtgtgg
    10501 ttctagccat tcgtgaagaa ctcaatattc ctgtaaaatt gattggtttt ggtgaaaaaa
    10561 tcgatgatat tggagagttt aactcagaaa acttcatgaa aggtctcttg gaaggtttaa
    10621 tctaatcaga agcaaaaatc ctgcaaggca taaacttgca ggaaattttt ttattcgaaa
    10681 cgaccatctt gacgataggc gatatctggt tgccaagtcc atttggcacc gaatttttca
    10741 agtaggtcaa agctggcttg aggtcccatg cttccagctt tatagtcatg aagtggggca
    10801 ccattttcag cccagagctc ttcaatacgg tcaatcaact tccatgatgc accaacttca
    10861 tcccagtggc taaagttagt tgagttgtta tttaggacat cataaatcaa tttctcgtat
    10921 ggttctggag aagcaccagt tgcagtcgca tctgtacggt aatcaagtga gttaggagcc
    10981 aagttaaatt cttctcctac ttgcttccca tttaggctaa gagagaagcc ttctgttggt
    11041 tgaatataga tggtcaaaat atttggagca agtggttctc caaagataga atccatttgt
    11101 ttaaagacga tgttgacatg agttcctttt tcagtcagtc gtttacctgt acggaaaaag
    11161 aaaggaacac tacggaatcg atcgctgtct acaaagaagg caccagatgt aaaggtttca
    11221 gttgttgatt ctggattcac atttggctcg ctacgataag agatgtattt catgccatca
    11281 atcttaccag agcggtattg cccacggata aagtgttctt tgagttcttc atcagttgga
    11341 tgatagaggt ttttaaagac cttaatcttt tcagcacgaa tctcgtcttt tgtgaagatt
    11401 gctggtttgt ccatggcgag gagcgaaaga agttgtagag tgtggttttg gaccatgtca
    11461 cggagggcac cggattggtc atagtagcca ccacgttctt ctacacccaa gcgctccgca
    11521 aaggtaattt gaacattgtc gataaaatcc ttgttccaaa cgttttcaaa aatcaagttt
    11581 gcaaagcgaa ctgcaaagat gctttggatc atttccttac caagataatg gtcgatacgg
    11641 aaaatttgtt cttcgtcaaa tgttgctagg agttcgtcat tcaacttgct tgcagttgcg
    11701 taatctgtac caaatggttt ttcaacgatc aagcgctcaa aacctttgcc atcgacaatg
    11761 ttttcagatt tgaggtgttt ggcaatggtt ccaaagaact gaggtgccat agacaagaag
    11821 aagagcttat tgtgttcagc ttggtatttt tcattaagct cagcttgtaa ttgacgcaaa
    11881 gcaatataat gttccgaatc attgacatca tggctttgat agtagaagtg gctagcaaat
    11941 tcttgggctt gctcggtact atctgccaaa tcaaggatgg actcgacaac tacagattca
    12001 aaatattcct tactccaagg tctacgggca gttccaataa cggcaaagtg cttggaaaga
    12061 ttgccggatt gatatagtct aaaaagggaa gggtagagct tgcgtttagc caggtctcca
    12121 ctcgcaccga aaattgtaac aataacctta gatgacatct agctacctaa tttctatttt
    12181 tattcctagt cttgctaggt atgaggaaac ctcatttcat aaacttaatt ctaacataat
    12241 tttccgaaaa atcaaaaatt ccaatacgag gtagaaaaac tgcaaggaaa tccttacagt
    12301 ttgagtttag acgtttaaga ccttatctaa gaactctttc agacgagggt gttgtgggtt
    12361 atcaaagatt tggtcaggtg ttccgtcttc aaggaactcg ccatctgcag taaagataac
    12421 gcggttggca acctgacgag caaatcccat ctcatgggtt acgataatca tggtcatgcc
    12481 ttgctcagcc aattccttca taacgttaag tacgtctcca accatctcag ggtcaagggc
    12541 agaagttggt tcatcgaaga gcatgatgtc tggattcatt gctaggccac gagcgatggc
    12601 cacacgttgt ttttgaccac ctgataggtt atctggattg gcattagctt tatctgctag
    12661 tccaaccttt tcaagcaact ccattcccaa ttcctcagct tcttccttag tcatcaactt
    12721 gtgctcaata ggagcaaagg tgatgttgtc caatacagac atatgaggga agaggttgaa
    12781 gtgttggaat accatgccga tattttcacg gacgtggtca acattggttg ttttttcagt
    12841 taaatcatag ccgttcacag tgatgtgacc gctagtgact tcttctaaaa gattgaggct
    12901 acggaggaaa gttgacttac cagaacctga aggaccgatg atacaaacaa catctccttc
    12961 atagaactta gtcgtaattc cttttaggac ttcatttttt ccatagtgct tgtgtaaatc
    13021 atttacatca atttttaatt ttgccattaa cgaatcctct tttctaagcg tttcgctagt
    13081 ctagtcaaaa gtgtgataat tacaagatag aagatagcaa ggattgcata catcttgaaa
    13141 ctttggtagt tacgagcaat gataatctta ccagtttgga agagttcaac caaaccgata
    13201 gcagatacga tagttgtatc tttaagagcg ataacgaatt ggttgacaaa gtttggcaac
    13261 atcaatttag ttgcttgtgg caagataatc ttacgcatgg tttttccata agagataccc
    13321 aagcttcggc tggcttccat ttggccaact ggaacggcct gaataccacc acgaacgatt
    13381 tcagcgatat aagcagccgc attgagtgag agggcaatgg ttccagctac aaagtcgtta
    13441 attgggcttt gttggcctgt gatagactcg atgaagtttg gaattcccca gaagatgaag
    13501 gctgcaagaa tcatcaatgg aataccacga ataacgtcaa cgaaaatctc agagatgacg
    13561 cgaagagatt tgtatgggct aacgctaaac ataccgaaga taattccgat gacaatggca
    13621 atagcaaatg agataagagc tagagcaaga gtgataccaa gaccgctaag gagttgtttg
    13681 tagttgtttt gaagcaagcc ccagagcgtt gtttcgtcaa cagtacttgt tgaagcagtt
    13741 gaagattcgc tagctaggta tttgtcaaga atcttttgga attcaccgtt tgctttaagg
    13801 tttgcaagtc cgttgttgaa catttcaatc agttctggat ttgctccttt tttaacggca
    13861 aaggctgttt caccgattgg agttccagag attggagttt tcaatttttg accttggctg
    13921 atagaatatt tgagaacagg ttcatcatcc ataacggcat caatggcacc agtgtttaaa
    13981 ctgtcataca ttgaagaacc atcagcaaag gttttgattt tgtaaccgta tttgctttga
    14041 ttttctgtta ggaaggtttg agaagcagtt ccgtttttaa caccgactgt ctttcctttt
    14101 agatcttcat aagaagcaat attgcttgat tctttgacac caagaatggt attagcagtg
    14161 tagtatgatt ctgagaagtc aaaagttgcc ttacgagcat ctgtgacaga cataccagca
    14221 atgataccat cggcttgacc agcttggaca gcactgatag cagcatcaaa accagggttg
    14281 gtgatttcaa tttcaaaacc ttggtcttta gcgattgcct taatcaattc catatcaata
    14341 ccagtgtatt ggttgcttga attttggaaa acaaaaggtg caaaagaaga atcgctggca
    14401 atgatatatt tagccttaac aggaatagct tttaatcctg cgagagtagt tgtagttggc
    14461 actgctgaag atgatgaagc agtccatttc ttgataattt tatcaagact accatctttt
    14521 ttcatttcag ccaaggcttg gttaaattca gtaaccaggt gctcgtattt acttcctttt
    14581 ttcacaccga aagcaaaact tcctacagct tcaccatcca tttcaatatg gaggtcttga
    14641 ccttggttaa tggcatattc gataacaggt ttgtcatcca tcatggcatc gatggcacca
    14701 gcactcaagc tgttgttcat taaatcacca gtgtcaaatg ttttaatagt aaagccgtat
    14761 ttatctttga ttgtttcaag gaaacgttga gcggcagttc cgtttttaac accaacggtt
    14821 ttgccagtta attggtcgta cttgctaatt ttgtgtgact ttgtagtagc aatgacaact
    14881 tttgtatcat agtaagtatc agacatggtg aagacttttt cacgttcttt agtctttgtc
    14941 atccctgcca tgatagcgtc ggcttgccca gcttgaaccg cattgactgc tgcgtcaaat
    15001 ccaggatagg acatctgaat gttccagcct ttaatctcag cgactttgtt aataatgtca
    15061 acatcaattc ctttataagt ttgatctgaa tctttaaact caaaaggtgc ataggcggta
    15121 tcagaaacaa tcttaatcgt ttctgctttg gcaataccta atgagaaaat tgggaataaa
    15181 attagcaaaa atgctagaaa ttttttcttc atttttagtc tccttttccg aatattctct
    15241 cattatatca gaaaaagtaa aaaaaggcaa atttttatat ttttcgtgtc agaaaatata
    15301 acattgattt ttccttttct ttcttgaatt gctattaaaa attgctataa taatactata
    15361 tatagaagaa aagaggacgg ggatatgaag aacaaaagaa tatttaaaga cttccaagct
    15421 tcaaaaatga atttaaacct ttacacaagc cccttgttag cctttgtttt tgtcttcata
    15481 ggagagtttg tggcttatac tttatatggt attagtttgt tagctctcat cggacttgct
    15541 agaaattttg gagaggctgg tcaaaatctt gcaagctact tgcagacctt gcatcagagc
    15601 ttgacggata aaacaagtga ctttcgttta attttaggat tactggcctt tggttttatt
    15661 cttaacactg tgttcagatg gacaagaaaa gttgagaaaa gacctattcg aaccttggga
    15721 ttttatagag agaattgcct cagcaatctt ctgaaaggat ttagtctagg cctggcactt
    15781 tttcttctga ccttgttaag tttagtggtc ttaggtcaat atcgtttgga atccattcac
    15841 ttgaatcctt attatcttgc ctttgtcgtc tttactatcc cattttggat tttacagggg
    15901 acagcagaag aagtggtggc ccgtgcttgg ctacttcctc aattggcctc aagaaccaat
    15961 ctaaaactag ctattcttat atctagcctg ttctttaccc tgcttcatat gggcaattct
    16021 ggcctcaccc ctctatctct agtaaatctc tttttattcg gagttgccat ggctctttac
    16081 cttctcaaaa ctgatacagt ttggggtgtt gcaggtattc atggtgcttg gaattttgct
    16141 cagggtaatc tctttgggat tttagttagt ggtcaaccgt caggaacgtc tctgatgacc
    16201 tttttaccac aaggcaatca agattggcta tcaggtggtt cttttggcat agaaggttcc
    16261 attatgacaa gtctggtatt actactgctg attgtctatc ttgctaataa attaaagaaa
    16321 gaaaatgaaa ggatgtgact tcggtccgtc cttttcttcg tgaaaatact ataagtatgc
    16381 taaaatagga atagcacatg gagagaggat tcttatgatc aatcacatta cagataatca
    16441 atttaaacta gtatcaaaat atcaaccatc aggagatcaa ccccaagcta tcgagcagtt
    16501 ggtggataac attgaggggg gagaaaaagc tcagattctg atgggggcga ctggaacagg
    16561 gaagacctat actatgagtc aggtcatttc taaagtcaat aaaccaactc tggttattgc
    16621 ccacaataaa actctggctg gtcagctcta tggggagttt aaggaatttt tccctgaaaa
    16681 tgcagttgag tatttcgtat cctactatga ttattaccag ccagaggcct atgtcccttc
    16741 tagcgatacc tatattgaga aggatagttc tgtcaatgac gagattgaca agcttcgcca
    16801 ctcagctacc tcagcccttt tggagcgtaa tgatgttatt gtcgtggcct cagtctcttg
    16861 tatctatggt ttgggttcgc ccaaggaata cgctgatagt gtcgttagtc tccgtcctgg
    16921 tctagagatt tctcgtgata aactcttgaa tgacttggtc gatattcagt ttgaacgcaa
    16981 tgatattgat ttccaacgcg gaagatttcg cgttcgtggg gatgtggtag agattttccc
    17041 agcttcccga gatgaacatg cctttcgagt agaatttttt ggagacgaaa ttgaccgtat
    17101 tcgtgaagtt gaggctctga caggtcaggt gttgggagaa gtggatcatt tagcgatttt
    17161 cccagcgaca cactttgtga ccaatgacga ccacatggaa gttgccattg caaagattca
    17221 ggccgagttg gaagaacaat tagctgtctt tgaaaaggaa ggtaaactgc ttgaagccca
    17281 gcgtttgaaa cagcggacag agtatgatat cgaaatgttg cgtgagatgg gctataccaa
    17341 tggggttgaa aattattctc gccacatgga tggacggagc gaaggagagc ctccttatac
    17401 gcttctcgac ttcttcccag atgatttctt gattatgatt gacgagagtc atatgaccat
    17461 agggcaaatc aagggcatgt acaatggaga ccgttcgcgt aaagaaatgc tggttaatta
    17521 tggtttccgt ttgccgtctg ctttggacaa tcgtcctctc cgtcgggagg agtttgagag
    17581 tcacgttcat cagattgttt acgtttcagc gacacctggt gactatgaaa atgaacagac
    17641 cgagacagtg attgagcaaa tcattcgtcc aacgggactc ttggatccag aggtggaagt
    17701 ccgtccgact atgggacaga ttgatgacct cttgggtgaa atcaatgccc gcgttgaaaa
    17761 aaatgagcgt acctttatca caactttgac caagaaaatg gcagaggact tgaccgacta
    17821 cttcaaggaa atgggtatca aggttaagta catgcactcg gatatcaaga ccttggaacg
    17881 gacggagatt atccgtgacc tgcgcttggg tgtctttgat gtcttggtcg gaattaacct
    17941 gctccgtgaa ggaattgacg ttcctgaagt gagcctcgta gctattctcg atgctgacaa
    18001 ggaaggtttc cttcgcaacg aacgtggact catccagact attggacgtg ctgctcgtaa
    18061 cagtgaaggc catgtcatca tgtatgcaga cacggttacc cagtctatgc aacgtgctat
    18121 cgatgaaact gcccgccgtc ggaaaattca gatggcctat aatgaagagc atggtatcgt
    18181 accacagaca atcaagaaag aaatccgtga cctgattgct gtgaccaagg cagttgctaa
    18241 ggaagaggac aaggaagtcg acatcaatag cctcaacaaa caagagcgca aagaactcgt
    18301 caagaaacta gaaaaacaaa tgcaagaagc cgtcgaagta cttgactttg aactggcggc
    18361 tcagatccgt gatatgatgt tggaagtcaa ggccttggat taggggaata gtatgattta
    18421 tttaagaaag ttaaagagag aagatttgat gtctttatgg gaaatggctt attcacaact
    18481 taatccagtt tggaaacagt atgatgctcc ctattatgat gattatcagt atttttcaaa
    18541 ttttaaagaa ttcgaactac aaaaatcaga atccatttta agcaactcaa atcgccttgg
    18601 tatttttgtt gatgataaac tagttgggac tgtttcgcgt tattgggtat gtaaacaaac
    18661 aagatggatg gaattgggaa ttggtattta tgataaaaaa ttctggaaca ctggtattgg
    18721 gaaagttgct atgttgcagt ggatagatag gacgtttcag gattacttgg agttggagca
    18781 tctgggtttg acaacttggt cagggaatct tggtatgatg aaacttgctg aaaaattaag
    18841 aatgaaaaaa gaagctcata ttccaaaagt tcgttattat caaggtaaat attttgatag
    18901 tattaaatat ggtattttga gagaagactg ggagaaaaca aatgacggtt attatcaaat
    18961 caatggaaac tcctgaagag atagaaggta agccccttgt tcactggcaa acgtggagag
    19021 aggcttatga tggccttttg cctgcagaat ttcaggagac aatgacatta gaaagatgtc
    19081 gattctttag tcaaaagtat ccagaaaata cattgattgc gatggatggt atgaaggtag
    19141 ttggttttat cagttatggc aactttcgtg atgagactat tcaagctggc gaaattattg
    19201 ctttatatgt tttaaaagac tattatggaa aaggtatcgc acaaaagtta ataaaagcag
    19261 ctttgattgc tcttaatcat ttttctgaaa ttttcttatg ggtattgaaa gataataagc
    19321 gcgcaattgc tttctatcaa aaaatgggct ttacttttga tgaacaagaa aagatacttg
    19381 aacttggaaa gcccataaag gaaaaacgga tggtattcta ttctaaataa ttttaaaaag
    19441 tagaagctaa tggtactggt atcaagtcta aaaatttaat aaattagaaa gtgagtaaat
    19501 ttatgtctcg agcacaatta acgattttaa caaatatttg tctgattgaa gatctcgaag
    19561 ctcagcgcgt ggtgatgcag tatcgcgctc ctgaaactaa tcgctggtct ggttatgcct
    19621 ttccaggagg ccatgtagaa aatggcgaat ctttcgcaga gtctgtcatt cgtgaaatct
    19681 atgaagaaac agggttgact atccaaaatc ctcaacttgt cggcattaaa aattggccac
    19741 tagatacagg tgggcgctat attgtcgttt gttataaggc aactgagttc tctggtaccc
    19801 ttcgctcttc agatgaagga gaagtttctt gggtgcaaaa agaccagatt ccaaacttgg
    19861 atctggccta tgatatgcta cctttgatgg agatgatgga agcatcggac aaatctgaat
    19921 ttttctaccc tcgccgtaca gaagacgatt gggaaaagaa aattttctaa tcctttacta
    19981 aataacctag ctgatccaag gcctcctcga tatagtggag gtcttgttgt gtttcggctt
    20041 cgactaggtg gtaatggaca ccatctgtca attcagataa aggtttaaaa tcagaatgct
    20101 cgacttgttc tagaaaatgt tgaacgtctc ggcggcaaga cagtttaagc aaggtttcaa
    20161 tttctccata aacgggatgg tcaatcaaag tattctggac atgcccacca ttatcgacaa
    20221 tagcaaggag ttcctgaccg atttcttcaa cttcatgttt cactttgaaa agtttgtgaa
    20281 cataaggatt ggtatcaatt tgcttataga tgtagccacg attggtggat aggataggag
    20341 cgccatcggc tctcaaaatt gcaatgtcct gcacaatgac ctgacgtgtg acatgaaaat
    20401 gttccgccaa actttggcca ttaagggctt taggagcttc tttcaagagt tggagcaggg
    20461 cttgtttgcg atcttttgtc atagcttttc cttttaacgg cgttttcgaa gcactttata
    20521 gacagctagt gctaatgtat agtctaccat actatggata attgtgccaa atccaactag
    20581 tacaaataga acatagaaca tgttttctac attggtaccc gaagttgcgt aaaaaaggac
    20641 acaggctaat acttcagcaa gggcatgaac aacagccaaa acaaaattga aaacccagga
    20701 agattttggt ttatctaggg tatcggggaa tttttgtagg taaagagatc ctaaagcacc
    20761 aaaagatata tgggaaaaag cccgaaaaac tataaccatg ggatagccag ccatcaaaaa
    20821 tccaaaacta gaggctagga tgacaaaaac tgccatcaag ggcgacaaga acatggctat
    20881 aaaaatagcg atgtggctcc ccaaagtata ggaagcaggt ggaatgacaa tcttgaaagg
    20941 cataacaatt ggaatcaaaa tcgcaatagc cgttaaaagg gctgtcattg tcataaattg
    21001 tgtctttttc cgtgtattca caagaatctc ctttttaact gcatatacac tagtatggta
    21061 caataaacca gacaataaag caagaattta cttgggttta tagatcattt tttagttaaa
    21121 agttatagta gatggaaact agaatagtcc acctctactt ctaaaacatt gttagcgatt
    21181 tgactgtcct gatcgatttg tcctgttctt atttcgtttt actatagtaa agatttcatt
    21241 aaaaagaaac tgtatagagc aaaatctcca ccttcaggtt tgaaagcgga gattgttttt
    21301 attttttcaa ggtttgtagt cgtggaacaa tggctagggt tagaattgca gaaatgacca
    21361 attcagcaat tgaatttgtt gagataacgg ttgccagaag aagttggata tttccattat
    21421 aaacatttcc aaataggaag aagattcctc caaggacaaa gatagtattt gtcaaggaac
    21481 caagggctcc agctaaaatc agaccagtct tgtttttcat cagtttatag actaagtaag
    21541 gagttaaacc aatcaaaata cgtgggacga tggcaatgat agctgagtag atgtttccgt
    21601 ttggtacgaa gggagagaag aggtagcttg tcggtagaat cgtaatcgtg ttaaccgtca
    21661 agctaagtaa tcccatcaaa aatccaagtg taaccccaac tcgtggacca taaataatgc
    21721 tggcaataat gacaggaata tgaacaatgg tcggtttgat tggaaatgga aaaaggttaa
    21781 agataagtga gctcagaaag tgtatcacga gcatggtagc aaaaaagata gcaatgggtg
    21841 caatattaga gcgttttttc atcgatagtt tcctttattc tttctaaaat aattgtgagg
    21901 tcagctaaag ctcctcgtcc gtggtctcca caagctagta gggattcctt aggagcaatc
    21961 agctgatagc cgtaggtttc taatgttttc agattattct gagttactgg atggtcatac
    22021 atttttgtat tcatagcagg agctattagt ttgggaatat gacttggtag ggctagagct
    22081 gtactggtta ccatgttgtc cgcaaatccg tgagctagtt ttgcaatagt gttagcagtt
    22141 gcaggtacca cgataaataa atctgctttt tttccaagtt cgatatgatt gacctgatca
    22201 ggatagggtt ccttcatgac atccaagtgg acaggattct gtgagagtac ctgtagtgtc
    22261 aaaggttgga taaactctgt agcagcctga gtcattaaga cagtgacttg atggccttgt
    22321 ttttttagag aactgactaa atctgccgac ttataagagg cgattgaacc cgttacagcc
    22381 aagagaatgt ttgccatagt tttcctttct aagaatgata ggcttgaatt ttttcaagga
    22441 ggagttctgc aatttcttct ttagtctgga ctgtttgaag ctgatttttc tcaacaaata
    22501 tagctcggtg ctgatctgct gaaatttgag tcaggtcatt cgcgatgatt aaatctgctt
    22561 gattcttgat aagacttttt cgtgcaatgt caaccagatg atcttcggta acatcaacca
    22621 gcagtttgaa accaatcaga tgaatagtgg gattccattc cttgactagg gatatgattt
    22681 tgggtgtctt tttaaggaac aaaacctgaa cctcatcagt tgaagaaatc ttggcctgat
    22741 gattctgctt gcttaaaaat tcttttagat tggagctagc ctgaacttcc tcaagccctg
    22801 tcatataaac aggagtgtag tcagaaacag ccattgagtg gatcaagacc tgataatcct
    22861 gaacacgttc ttgcatttct attagaaggt ccttggtatt ggtaatttct cgaatactta
    22921 ggttaggatg aggctctggc ttcagagctc gttttgtcgt aattaaacaa acttcatgcc
    22981 ctgcagaaag caaagtctct gtgataattt tccccaagtg acctgtagaa tggttagtga
    23041 tagagcggac gctatcgata gcttcactgg taccgcccga tgtaactaaa attttcatag
    23101 cactattgta cacaaaaata agcggtaagt aaaatgaaag tgataaattt ttggtaaaaa
    23161 cgaagattta ctatagtttc aaactataaa gccatataaa atttaagaaa ggactctaaa
    23221 agccttaaaa acagagatat ttacgaacgt ttagagagtt ttaggatgtt aaaaatcttg
    23281 ttttgtgata taatgaatta tcatataaga ggttagagga attttgaatg aaaacagata
    23341 ttgaaatcgc acagagtatt gagttgaagc caattgttga tgttgtagag aaacttggta
    23401 tttcttatga cgatttggag ttgtacggaa agtacaaggc taaactcagc tttgataaaa
    23461 ttcgtgcagt tgagagcaat ccagtcggta aattgatctt ggttactgcc atcaacccaa
    23521 cacctgcagg tgaaggaaag tcgacgctta ccattggtct tgcggatgcc ttgaacaaga
    23581 ttggcaagaa aaccatgatt gctatccgcg aaccgtctct tggtccagtc atggggatca
    23641 agggtggtgc tgctggtggt gggtatgcac aagttctgcc aatggaagac atcaacctcc
    23701 actttactgg agatatgcat gctattacaa ctgccaacaa tgccctttct gccttgattg
    23761 acaaccactt gcaccaaggg aatgagctgg aaattgatca acgtcgtatc ctctggaaac
    23821 gcgttgtgga cttgaacgac cgtgcgctcc gccatgtgac tgttggtctt ggtggtcctc
    23881 taaacggtat tccacgtgag gatggttttg atattacagt tgcttcagaa atcatggcaa
    23941 ttctttgctt ggcaacggac atcgaggact tgaaacgtcg tttggcgaat atcgttattg
    24001 gttatcgcta tgaccgtacg cctgtttctg taggtgattt gcaggttgag ggtgccttgg
    24061 ctttgatttt gaaggatgct attaagccaa acttggttca gacaatttac ggtacacctg
    24121 cctttgtaca cggtggtcca tttgccaata tcgctcatgg ctgtaactct gttttggcga
    24181 cgacaacagc ccttcacttg gctgattaca ctgttactga agctggtttt ggtgcggacc
    24241 ttggtgctga gaaattcctt gatatcaaga caccaaactt gccaacatct ccagatgcag
    24301 ttgtcattgt cgcaaccctt cgtgccctta agatgaatgg tggtgtggct aaagacgctc
    24361 tgactgaaga aaatgtagag gcagttcgtg caggttttgc taacttgaaa cgccacgttg
    24421 aaaatatccg taagttcggt attccagcag ttgtagctat taacgaattt gtatctgata
    24481 cagaagctga aattgcagtc ttaaaagaac tctgtgcctc aatcgatgta ccagttgaat
    24541 tggctagtgt ctgggctgat ggagcagaag gtggagtagc acttgccgaa acagttgtta
    24601 agacaattgc tgaaaatcca gctaactata aacgtttgta cgataatgcc ctttctgtcc
    24661 aagaaaagat tgaaaaaatt gtcactgaaa tctatcgtgg tagcaaagtg aactttgaga
    24721 agaaagctca aacacaaatt gctcaaatcg ttcaaaacgg ttgggacaaa ttgccaatct
    24781 gtatggctaa aactcaatac agtttctcag acaatccaaa tgcgcttgga gcaccagaaa
    24841 actttgaaat taccattcgt gaattggtac caaaattagg tgcaggcttc atcgttgcct
    24901 taactggtga tgttatgacc atgccaggtc ttccaaaacg tccagcagct ctcaacatgg
    24961 atgttgaaag cgatggaact gtactaggct tgttctagtg gattgaaatt gactttaagt
    25021 gagtttggaa ataatatcca agctcatttt ttctcagact gcaagcagtt catcaataag
    25081 atttatactc ttcgaaaatc tcttcaaact acgtcagctt caccttgccg taggtatatg
    25141 atactgactc tgtcagttct atccacaacc tcaaaacagt gttttgagca acctgcggct
    25201 agcttcctag tttgctcttt gattttcatt gagtataagt agatagaaaa gttatatact
    25261 atctattgaa aatattataa atcagataca aagacaggtg aaaccttctg tgttatacta
    25321 gagatatgtt agatttgaaa gaatacggta tcgtcatgtg gccggaggag aaagtcattt
    25381 ctttccgtga gaaacttctc gcttggtatg atgaaaacaa aagagatttg ccttggagga
    25441 gaagtaaaaa tccttatcac atctgggtat ctgaaatcat gcttcagcag accagggtgg
    25501 atacagttat cccttactac gaaagattcc tggactggtt tccaactgtc gaaagtctgg
    25561 caactgcgcc tgaggagagt ttactgaaag cttgggaggg cttgggctat tattctcgag
    25621 ttcgcaatat gcaggctgca gcccagcaga ttatgactga ctttggtggc caatttccaa
    25681 atacctatga aggaatttcc agcttgaaag ggattggacc ttacacagca ggagccattt
    25741 ccagtattgc ttttaacttg cctgagccag ctgtagatgg taatgtcatg cgggtcttgg
    25801 cgcgtctgtt tgaagtcaac cacgatattg ggattccaag taatcgaaaa atttttcagg
    25861 caatgatgga gatcttgatt aatccggatc gtccgggtga ttttaatcaa gccttgatgg
    25921 acttagggtc tgatattgag tctcctgtaa atcccagacc tgaagaaagc ccagtcaagg
    25981 actttagtgc ggcatatcag aatggaacca tggaccgtta tccaattaaa tctcctaaga
    26041 aaaagccggt tcctatttat cttaaagcat tagttgtgaa aaattctcaa ggacaatttt
    26101 tacttgaaaa aaatgaaagc gaaaagctct tggcaggttt ttggcatttc ccccttatag
    26161 aagttgataa cttttcgcaa gaagagcagt ttgacctctt tcatcaggtt gcggaagaaa
    26221 gtgtgaactt tggccccagt ccagaagaga gtttccagca ggactatgac ctagatgttg
    26281 attggcttga tgtttgtttt gatacagtcc agcatgtttt tagtcatcga aaatggcatg
    26341 tacagattgt agcaggtcag gtgagtgact tccatgattt ttcagatagg gaagttcgtt
    26401 ggctttcacc agaagaattc aagaattacc cacttgccaa accccaacaa aaaatctggc
    26461 aggcttatgc acaagccaac ttagacagta gccaagacta gctattgtgt cttctgacta
    26521 ttttttggta taatagtaga gaaaaaggtg aacatatgaa aaaaatacta attgtagatg
    26581 atgagaaacc aatctcggat attatcaagt ttaatatgac caaggaaggt tacgaagttg
    26641 taactgcttt taatggtcgt gaagcgctag agcaatttga agcagagcaa ccagatatta
    26701 ttattctgga tttgatgctt ccagaaattg atggtttaga agttgctaag accattcgta
    26761 agacaagcag tgtgcccatt cttatgcttt cagccaaaga tagtgaattt gataaggtta
    26821 tcggtttgga acttggggca gatgactatg taacgaaacc cttctccaat cgtgagttgc
    26881 aggcgcgtgt taaagctctt ctgcgtcgtt ctcaacctat gccagtagat ggtcaggaag
    26941 cagatagtaa acctcaacct atccaaattg gggatttaga aattgttcca gacgcctacg
    27001 tggctaaaaa atatggcgaa gaactagact taacccatcg tgaatttgag cttttgtatc
    27061 atttagcatc gcatacaggt caagtcatca cgcgcgaaca cttgcttgag actgtctggg
    27121 gttatgacta ttttggtgat gtccgtacag ttgatgtgac tgtacgacgt ctgcgtgaga
    27181 agattgaaga tacgcccagc cgaccagagt atatcttgac gcgccgtggt gtagggtatt
    27241 acatgagaaa taatgcttga tttactgaaa caaaccattt ttaccagaga ttttatcttt
    27301 atcctgattt tgttaggttt catccttgtt gtgaccctct tattactgga aaatagacgt
    27361 gataatattc agttgaagca aatcaatcaa aaggttaaag atttgattgc aggagattat
    27421 tccaaggttc ttgatatgca aggtgggtct gaaatcacca atattaccaa taatttgaat
    27481 gacttgtcgg aggttattcg tctcactcag gaaaatctag aacaagagag taagaggcta
    27541 aatagtattc tgttttatat gacagatggg gttcttgcga ccaaccgtcg gggtcagatt
    27601 atcatgatta acgatacagc caagaagcaa ctggggttgg ttaaggaaga tgttctgaat
    27661 agaagcattt tggaattgct caagatagaa gaaaactatg aattgcgtga tttgattacc
    27721 caaagtccag aattgttgct agattcccaa gatatcaatg gcgaatattt gaaccttcga
    27781 gttcgctttg ccttgatacg tcgagagtct ggctttattt caggtttggt ggctgttttg
    27841 catgatacga cggagcagga gaaggaagaa cgcgaacgaa gactctttgt ttccaatgtt
    27901 agccatgagt tacggactcc tctgactagc gtaaaatcct atcttgaagc cttggatgag
    27961 ggggctttgt gtgaaactgt agcaccagac tttatcaagg tttctcttga tgagaccaac
    28021 cgtatgatgc gcatggtgac ggatctcctc catctttcac gtattgataa tgctaccagt
    28081 cacctagatg tggaactgat taacttcact gcttttatta cctttatcct caatcgtttt
    28141 gacaagatga aaggacagga aaaggagaaa aaatatgagt tggtgagaga ttatcccatc
    28201 aattctatct ggatggaaat tgatacagat aagatgacgc aggttgtcga caatatttta
    28261 aataatgcta ttaagtattc gccagatggg ggtaaaatca ctgtcagaat gaagacaact
    28321 gaagaccaga tgattttatc catttctgac cacggtttgg ggattcctaa gcaggattta
    28381 ccacgtatct ttgaccgttt ctatcgtgtg gatcgtgcta gaagtcgtgc acaaggtggt
    28441 acaggtctag gactgtctat cgctaaagaa attatcaaac aacataaggg ctttatttgg
    28501 gccaagagtg aatacggcaa gggttcaacc tttaccattg tgctccctta tgataaggat
    28561 gcagtgaaag aagaagtatg ggaggatgaa gtagaagact agaatgagtg aaataggctt
    28621 taaatacagt attttagcgt cgggttccag tggaaattct ttttatctgg aaacctcaaa
    28681 aaagaagctt ttagtagatg caggcttgtc tggcaagaaa attaccagtc tgctagctga
    28741 aattaaccgt aagccagaag acctggatgc catcttgatt acccatgagc attcagatca
    28801 tatccatgga gtaggcgttt tggctcgcaa gtatggtatg gatctttatg ccaatgaaaa
    28861 gacctggcaa gctatggaaa atagtaaata tcttggcaag gtggattctt cgcaaaagca
    28921 tatttttgaa atgggtaaaa ccaaaacctt tggagatatc gacatcgaga gttttggtgt
    28981 aagccatgat gcagtcgcac cgcagttcta tcgctttatg aaggatgata agagttttgt
    29041 cctcttgaca gatacaggtt atgtcagtga ccgtatggcg ggcattgtcg aaaatgcgga
    29101 tggttatctt atcgaggcca accatgatgt agagattttg cgatcaggtt cttacgcttg
    29161 gcgcctcaaa caacgaatcc tatctgacct tggtcacctt tctaacgagg acggtgctga
    29221 agctatgatt cggacgctag gaaatcgtac taagaagatt taccttgggc atttatctaa
    29281 ggaaaacaat atcaaggaac tggctcatat gaccatggtc aatcagctgg ctcaagctga
    29341 tctgggagtc ggagtagact ttaaggttta tgatacctca ccagataccg caacaccatt
    29401 gacagagata tagaaagaac gctgagaagg tgttcttttt atattgactg gacacctaaa
    29461 aagtaataca atggtgttac cattaaaaaa gggagcacaa aagatgacta ctattacatt
    29521 aaaagtttct gaagctgata aaacatttat gaaagcaatg gctaagtttg aaggagtttc
    29581 cctttcggaa cttattcgca ccaaaactct tgaagcccta gaagatgaat acgatgctcg
    29641 tgtggcagat ttagcctatc aagagtattt agaagacttg gaaaaaggag ttgaacccat
    29701 tacttgggaa gaaatgatgc atgatttagg cttgaaggat gaataatttg tataaattag
    29761 ttccaacaag acgttttatc aagcaattga aaaaattgga ccgttatacg cagaagctaa
    29821 ttacaaacta tttacaaacc aatgttttgg aagatccaag acgacacgga aaggctttgg
    29881 ttggtaatcg cgttggtcaa tggcgctata gaattggtaa ttatcgagtt atcgtacaaa
    29941 ttgtagatga tgaattagtt gttgctactc tagaagttgg tcatcggaga gatatttatt
    30001 aaattaaagc tgagtaataa cagtatagaa agtaaggaga gtaaaaattt gaagaaaaaa
    30061 tggttttttg cagattatta tgacacaaca attattttac ttgctttaat ctctgttatt
    30121 ctggtattac ttggatttgc tgagatgatt gatttggaca atccgcctta tagtattatt
    30181 gatttagtaa tctggggtgt ttttgtaatt gattatagtt ggcgcttttt tacgactaaa
    30241 agaaaatggc gctttattct tgaaaatatt tttgatttat tggctattct ccctttaaat
    30301 gctattttta cagtgtttcg attagggcgt atctttcgtt tagctaaact aacaaaatta
    30361 ctgaaactga ctcgtttact tagaataatt ggacttacag gtaaattaga aagaaaaatt
    30421 agtagattct tacggacaaa tggcctgatt tatatcttgt atgttaatat ctttattgtt
    30481 ctagtaggga gttcgatttt atccgttgtt gaagaaaaat ccttctcaga tagtctttgg
    30541 tgggctcttg tgacagtaac tactgttgga tatggggata ttgttcctgc ttccattttt
    30601 gggaaatggc tagcagtttt actgatgctt gtcggtattg gaacaatagg gatgttaaca
    30661 agtgctttga cgaacttttt tgtaaaggat aatccagatg aacagataaa acttgataaa
    30721 ctcaaagatg aattatcgag ccaaagaata ttactagaga aacaatctaa aaagatagaa
    30781 gaactacata aaatgataca agatttgatt gaaaaaacat aataaaaata aagatgagac
    30841 aaagtttgag ctgaggagaa atcaagtaag ttttgacaca atagaacgca tattatcagg
    30901 tataaaaact tgatactatg cgttttatag agaaataaaa taaaatctga acaaatcaat
    30961 taggaattta aagcatttta taacaacgtt ttagagtgat ggggtgctat ttcaacttca
    31021 acctactata atacagaaaa aaacaactcc ctgatgattc aaggagttgt ctatagttaa
    31081 attagttttt agaagcttct tggaattctg gatttttcca tgcttcgtca ataatagctt
    31141 gcaattcttt agcagatgct tgcatttttt gagtttctgc atcgttcaat ggaatgttta
    31201 ctggacgaac gataccatgt gcaccaacaa cagctggttg accgataaag acattctcaa
    31261 ctccgtatag accttcttgg aatactgaaa gtggaagtac tgcgttttca tcgtcaagga
    31321 ttgctttagt gatacgagca agggctactg cgataccgta gtatgttgca ccttttttgt
    31381 tgatgattgt gtaggctgca tcacgaacac cttcgaacaa ttcaatcaat tcagcttctt
    31441 gaacattttg agtgtcttta aggaattctt caaggtttac accagcgatg ttagcgtgtg
    31501 accaaacagc gaactcagag tcaccgtgtt cacccatgat gtaggcgtgc actgaacgag
    31561 catccacatc caatttttca gcaagtgctt gacggaaacg agctgagtca agtgaagtac
    31621 ctgaaccgat aacgcgttct ttagggaaac cagagaattt ccaagttgag taagtcaaaa
    31681 cgtcaactgg gttagcagca acaaggaaga tacctttgaa accagattca acaacttgag
    31741 ttacgattga tttgttgata gcaaggtttt tacctacaag gtcaagacga gtttcacctg
    31801 gtttttgagg tgcacctgca gtaatcacaa caaggtcagc gtctgcacag tcagagtatt
    31861 gagctgcata gattttttta ggtgaagtga aggcaagggc gtgactaagg tcaagcgcat
    31921 caccaacagc tttttcatgc aattgtggaa tttcgataat tccaagctct tgtgcaattc
    31981 cttggttaac aagtgcaaaa gcgtaagatg aacctacagc accatcaccg acaaggataa
    32041 cttttttgtg ttgtttagtt gaagtcattg ttttaaacat ctccttaatt ttattagggg
    32101 attttcccta gacaacttca ttctatcact tttaaaaaac tttgtcacga atatgcctta
    32161 tagttctcga tgtaaacgtt ttagtggttt agaggctgaa atagatggga atttatggta
    32221 taatgttgtt acttactaat tgtgaaatga ggcatttatt aatgcaggat aaaaatttag
    32281 tgaatgtcaa tctgacaaag gagatgaagg caagttttat cgactacgcc atgagtgtta
    32341 tcgtagcgcg agctcttcct gatgttcgag atggcttaaa acctgttcac cgtcgcattc
    32401 tctacggaat gaatgaattg ggtgtgaccc cagacaaacc ccataaaaaa tctgctcgta
    32461 ttacagggga tgtcatgggt aaatatcacc cacacgggga ttcctctatt tatgaagcca
    32521 tggtccgtat ggctcaatgg tggagctacc gttacatgct tgtagatggt catgggaatt
    32581 ttggttccat ggatggagat agtgctgccg ctcaacgtta taccgaggca cgtatgagca
    32641 agattgctct ggaaatgctt cgtgatatca acaaaaatac agttgatttc gttgataact
    32701 atgatgccaa tgaacgggaa cccttggtct tgccagcgcg ttttccaaac cttttggtta
    32761 atggagcaac tggtatcgcg gttgggatgg caaccaatat tccacctcat aatctgggtg
    32821 aaaccattga tgcagtgaag ttggtcatgg ataatcctga agtgactacc aaggacttga
    32881 tggaagtctt gcctggacca gattttccaa ctggtgctct tgtcatgggg aaatcaggta
    32941 tccataaggc ttatgaaaca ggtaaaggtt cgattgccct acgttctcgt acagagattg
    33001 aaacgactaa gactggtcgt gagcgcatcg ttgtaacaga atttccttac atggtcaata
    33061 aaaccaaggt gcatgagcat attgttcgct tggttcagga aaaacgcatt gagggtatca
    33121 cagcagtacg tgatgagtca aaccgtgaag gtgttcgatt tgttattgaa gtcaagcgcg
    33181 acgcctcagc caatgttatt ctcaataacc tcttcaaaat gacccaaatg caaaccaatt
    33241 ttggtttcaa tatgctcgct atccaaaatg gtataccgaa aattttgtct cttcgtcaga
    33301 ttttggatgc ttatatcgag caccaaaaag aagtggttgt tcgtcgtaca cgttttgata
    33361 aggaaaaagc ggaagcgcgc gctcatatct tagaaggtct cttgattgcg ctagaccata
    33421 tcgacgaagt gattcgtatc atccgtgcta gtgaaacgga tgcggaagct caagctgagt
    33481 tgatgagcaa gtttaagctt tctgaacgtc aaagtcaagc tatccttgat atgcgtcttc
    33541 gtcgtttgac aggtttggaa cgcgataaga ttcaatctga gtatgatgac ctcttggctc
    33601 tgattgcgga tttagcagat attcttgcta agcctgaacg tgtttctcaa attatcaaag
    33661 acgaattgga tgaagttaaa cgtaaatttt ctgataagcg ccgtacagag ttgatgattg
    33721 gacaggtctt gagtctcgag gatgaggact tgattgaaga atcggatgtc ttgattaccc
    33781 tttctaacag aggctacatt aagcgtttgg atcaggacga gttcactgct caaaaacgtg
    33841 ggggtcgtgg tgtccaagga acgggagtga aagatgatga ctttgttcgt gagttagtgt
    33901 caactagcac ccatgatcat ctgctcttct tcacaaacaa gggacgtgtc tatcgtctta
    33961 aaggttatga aattcctgag tatggtcgga ctgccaaagg gctaccagta gtcaatctct
    34021 tgaaattgga tgaagacgaa agtattcaga cggttatcaa tgttgagtct gatcgcagtg
    34081 atgatgctta tctcttcttt acaacccgtc acggtattgt gaagagaacc agtgttaagg
    34141 agtttgccaa tattcgtcaa aatggtctca aagcgctgaa tttaaaggat gaagatgagt
    34201 taatcaatgt cttgttggca gaaggagata tggatattat cattggtacc aagtttggtt
    34261 atgcagttcg ctttaatcaa tcagccgttc gtggtatgag tcgtatcgcc actggtgtga
    34321 aaggtgttaa ccttcgtgaa ggagacacag ttgttggtgc cagcttgatt actgatcaag
    34381 atgaggttct tattatcaca gaaaaaggat atggtaagcg tacagtcgct actgaatacc
    34441 caacaaaagg tcgtggtggt aagggaatgc agacagctaa aattaccgaa aaaaatggct
    34501 tgctggccgg tcttatgact gttcaagggg atgaggattt gatgattatc actgatacag
    34561 gtgtcatgat tcgaaccaat cttgccaata tttcacaaac aggacgtgca actatgggag
    34621 ttaaagtaat gcgcctggat caagatgctc agatagtgac tttcacaacg gttgcggtgg
    34681 cagaaaaaga agaagttggg acagaaaacg aaacagaagg tgaagaataa tgtctcaaaa
    34741 aaataataaa aagaaaaaca agcgaaaaaa tctgctgaca aatatcctag caggatttct
    34801 gatattactg tcactggctt tgatttttaa tactcaaatt cgaaatattt tcatagtctg
    34861 gaataccaat aagtatcaag ttagccaggt atcaaaagaa aaattagaag aaaatcagga
    34921 tacagaaggc aattttgact ttgattctgt caaagctatc tcttcggaag ctgttctaac
    34981 ttctcaatgg gatgctcaaa aattaccagt tattggggga attgcaattc ctgaattgga
    35041 aatgaatttg ccgattttta aaggacttga taatgttaat ctcttctacg gagctggtac
    35101 aatgaaacgc gagcaagtaa tgggagaagg aaattatagt ctagctagtc accatatctt
    35161 tggtgttgat aatgctaata aaatgttatt ttctccttta gataatgcta aaaatggcat
    35221 gaagatttat ctaaccgata aaaataaagt ttatacttat gaaatacgtg aagtcaaacg
    35281 tgtgacaccg gatcgtgttg atgaagttga tgatagagat ggggtcaatg aaatcacatt
    35341 agtaacctgt gaagaccttg ctgctacaga acgtattatt gtcaaaggtg atttgaaaga
    35401 aacaaaagat tattcacaaa catctgatga aattctaaca gctttcaatc aaccatataa
    35461 acaattttat taatacaaat cagtgaaatc atggatttca ctgatttttt aagattttca
    35521 taaaaatata gtaaaatgaa ataagaatag gacgaatcgt tcaggacagt caaatcgatt
    35581 tctaacaatg ttttagaagt agaggtgtac tattctagtt tcaatctact ataaactttt
    35641 atgaaataca gaaaacgctt cctaaaacct aaagtttgtg atataattat caagaaaaaa
    35701 gatttaggag tttattatgg tttcttcaga atttatctca aagattgaat ttgcttgcaa
    35761 taagaaagaa agtctttata gtcaaagcaa atttaagtat gcgattcgtt cgatgttcgc
    35821 aggtgcattt ttaaccttca gtactgctgc aggtgcagtt ggggctgact tgattaataa
    35881 aattgcacca ggtagtggac gcttcctctt tccattcgtt tttgcttggg gcttggccta
    35941 cattgttttt ttgaatgccg agttggtcac ttcaaacatg atgttcttga ctgctggtag
    36001 tttcttaaaa aaaatctctt ggagaaaaac agctgagatt ttactatact gtaccttgtt
    36061 caaccttatc ggagccttga tagcagggtg gggctttgct cattcggcag cctatgcgaa
    36121 tctgacacac gatagtttca tctcaggtgt tgttgagatg aagttaggcc gctcaaatga
    36181 attggtcttg cttgaggcga ttttggcaaa tattttcgta aatattgcga ttctgtcatt
    36241 tattttggtc aaagatggtg gtgccaaact ttggcttgtg ttgtcagcta tttacatgtt
    36301 tgtattctta acaaacgagc acattgcggc gaactttgct tctttcgcga ttgtgaaatt
    36361 cagtgttgct gcggattcaa ttgccaactt cggtgttgga aatatgcttc gccactgggg
    36421 tgtgactttc atcggaaact ttatcggagg aggcctcttg atgggtcttc catatgcctt
    36481 cctcaataaa aacgaagata cttatgtaga ttaagaaaat gagcacgatt gagtcgtgct
    36541 tttttcattt tcaaaataag gtaatagcta tttcttatat caaaatatag aaaactgata
    36601 tttgtaaact ataactcaag gtgctacaat atccttaata aaatgatatg gaggtcacct
    36661 tatgacttgt gattttaaat ttgaaactct acaactacat gctggtcaag ttgtggctcc
    36721 agctactaag tctcgtgcag tgccgattta tcaaacaaca tcttttattt ttgatgacac
    36781 gtaggaaggt gctgatctgt ttgccttgag gaaaccaggg aacatttata ctcgtatcac
    36841 caatcctaca acagctgccc ttgaaggtgg tgttgaagcg ctagcaacag catcaggtat
    36901 gactgcagtg acttatacga ttttggcgct tgcccatgct ggtgaccatg tagtggctgc
    36961 ttcgactatt tacggtggaa ccttcaatct tttgaaagaa ccccttcctc gttatggtat
    37021 cacaacaacc tttgtcgatg ttgataattt ggaggaagta gaagcagcta tcaaagacaa
    37081 taccaagctt gtcttgattg aaaccttggg taactccttg attaatattc cagacctgga
    37141 aaaactggca gagattgctc ataaacatca aatcccactt gtgtcagaca atacttttgc
    37201 aataccttat ttgattaacg tcttctctca tggcgttgac attgccattc actctgcgac
    37261 taagtttatc ggtgggcatg gtacaactat tggaggaata attgtcgata gtggtcgttt
    37321 tgactggacg gcttcaggga aattccctca atttgttgac gagggtccaa gctgccacaa
    37381 tttgagttat acttgtgatg tgggtgcagc agcctttatt atagctgttc gagttcaatt
    37441 gcttcgtgat acaggtgcag ccttgtcacc attcaatgct ttcctcttgc tacaaagact
    37501 tgaaacctct tcacttcgtg tggaacgcca tgtacaaaat gctgagacaa ttgttgattt
    37561 tcttgtcaac catcctaagg tagaaaaggt aaattatcca aaacttgcag atagtcctta
    37621 tcatgccttg gctgagaaat acttgccaaa agatgtcggt tcaatcttta ccttccatgt
    37681 caaaggtggc gaggaagaag cacgcaaggt cattgataat ttagaaatct tttctgacct
    37741 tgcaaacgcg gcagatgcta aatcgcttgt tgtccatcca gcaacaacca ctcacggtca
    37801 attgtcagaa aaagacctag aagcagcagg tgtcacacca aactaaattc gtttgtcaat
    37861 cggtcttgaa aatgtagaag atttgattga agacttgcgc ttggccttgg aaaaaattta
    37921 aagtaaaaga agataaacag tgggcttcga ctcactgttt ttgattttcc ctcaggcatg
    37981 atataatggt taaagaagtc tagaaagagg aacgatatga acgaaatcaa atgtcccaac
    38041 tgtggggaag tctttacagt aaatgagagt cagtatgccg aactcttgtc ccaagtgaga
    38101 acggcagagt ttgataagga actacacgat aggatgaagc aggaactggc cttggctgag
    38161 caaaaggcca tgaatgagca acagactaaa ctggctcaga aggatcaaga aattgcgcaa
    38221 ttacagagtc agatccaaaa ctttgataca gaaaaagaat tggccaagaa agaggttgaa
    38281 cagacaagcc atgaggctct cttggctaag gacaaggaag tacagctctt agaaaatcag
    38341 ttggctacct tgcgtttgga gcatgaaaat caactgcaaa agaccctttc tgacctagaa
    38401 aaagaacggg atcaggttaa aaaccaacta cttttgcagg aaaaggaaaa tgaattatct
    38461 ttggcttctg ttaagcaaaa ctacgaagcc cagctcaagg cagctagtga acaagtcgag
    38521 ttttataagg attttaaggc tcaacaatct acaaaagcga ttggggaaag cctagaacag
    38581 tatgcagaga gtgagtttaa caaggttcgt agtttcgcct ttccaaatgc ttactttgag
    38641 aaggataaca aggtctcttc gcgtgggtct aaaggggact ttatcttccg tgagtgtgat
    38701 gaaaatggag ttgaaatcat ttctatcatg tttgagatga aaaacgaagc agacggaaca
    38761 gagaagaagc acaagaatgc agatttttac aaggaattgg acaaggaccg tcgggagaag
    38821 aactgtgagt atgccgtttt ggtgaccatg cttgaggccg ataatgacta ctttaacaca
    38881 gggattgttg acgtcagcca cgagtatgaa aaaatgtatg ttgttcgtcc tcaattcttt
    38941 atccagttga ttggtctctt acgtaatgcg gcgctaaatt ccctaaaata caagcaggag
    39001 ttggccttgg ttcgcgagca aaatattgac attacgcatt ttgaggaaga cttggatgcc
    39061 tttaagctag cttttgctaa gaactataat tcagcttcga ctaactttgg aaaagctatt
    39121 gatgaaatcg acaaggccat caaacgcatg gaagaggtta agaaattcct gactacatct
    39181 gaaaaccaac tccgtctagc taacaacaaa ttggaagatg tctctgttaa aaaattgacc
    39241 cggaaaaatc caacaatgaa agcgaagttc gaagcactga agggggagta gaaagcaaaa
    39301 atgaacggta ttatcaactt aaaaaaagaa gcaggaatga cctcgcatga tgcggttttt
    39361 aaactgcgta agattttggg aaccaagaaa attggtcatg gtggaacctt ggatccggat
    39421 gtggtgggtg ttttgccgat tgcggttggc aaggcgacac gcatggtcga gtttatgcag
    39481 gacgagggta agatctatga gggggaaatc attctgggct attccacgac gactgaggat
    39541 gctagtgggg aagtggtcgc agaaacccct gttttgtctc ccttggatga aaagcttgtt
    39601 gatgaagcga ttgctagctt gactgggcct attactcaga ttccccctat gtattcggca
    39661 gttaaggtta atggtcgcaa gctctatgag tatgcgcgtg ctggtcagga agtggagcgt
    39721 ccagaacgtc aggtgaccat ttatcaattt gagcgaacaa gtccgatttc ttatgatggc
    39781 caacttgccc gattcacttt tcgtgtaaaa tgcagtaaag ggacttacat ccgtactttg
    39841 tcagttgatt tgggtgaaaa gcttggttat gcggctcata tgtcccattt gactcgtact
    39901 agtgctgctg gcttacaatt agaagacgct cttaccttgg aggaaattgc tgaaaaagta
    39961 gaggctgggc aattagattt tctccatcct ttagagattg ggacaggtga ccttgtcaaa
    40021 gttttcctaa ctccagaaga ggctacagaa gttcgctttg gtcgttttat tgagctagac
    40081 caaacggaca aagaactggc tgcctttgaa gatgataaat tgttagccat tctagaaaaa
    40141 cggggcaatc tctataagcc aaggaaggtt tttagctaga tcgtttagga ataaaaatcg
    40201 ggtgatagat aacaattgct tgataaaccc ccatactaat agtagaatgg ttttgggaat
    40261 tataatattc caattgttgc gagttgtaga tactcaaata atctatatag aaatttagag
    40321 gtgtgaaatg aagcaactta aaattctttc agataaatat ttagagtcca tgacaggttc
    40381 tggtgggaac ttaggtctag gatttggtgt gataattcca tgatgtgaaa tgagtttcga
    40441 gaaagggtgg agcaacttct tcaacaaaaa gaaataaatg aaaatagtga gttgagtcac
    40501 ctgtttcgtc ttgctataca aaatttagac agaaatgaaa aataccaatc ggtcatggcc
    40561 aatttgagtc aagggttgtc actttacctc atgacgcatc attaccaggc acctaagtct
    40621 gtcattgatt ttggtttatg gattgccaaa gctcctagcc aggaaagagg gagactggct
    40681 ttcttgcaga tgcttgctca aaccctacag ggctttcgtt aaaactgtcg aacatcagag
    40741 ggaagactct ggtgtttttt gctgatgaga ctataaattt tgtaaaaaat gtgatagaat
    40801 agacgacgga taaaataacg gaggatagca tgcaaaatag accaatcatt attggagtga
    40861 caggtggttc tggtggtggt aaaaccagtg tttcaagagc cattttatcg cattttcctg
    40921 atgaaaagat ttccatgatt gagcatgatt catactacaa ggatcagtct catttgacct
    40981 ttgaagagcg tgtcaaaacc aactatgacc atccttttgc ctttgataca gacttgatga
    41041 tcgagcagat taaggaattg ttggcagggc gtccggtgga catcccgacc tacgactata
    41101 cagagcatac acggagtagc aagacctatc gtcaggagcc tcaagatgtc tttatcgttg
    41161 agggcatttt ggtcttggag gacaagcgtc tgcgcgattt gatggatatc aagatttttg
    41221 tggatacgga tgacgatgtg cgtattattc gtcgtatcaa gcgtgatatg gaggaacgtg
    41281 gccgtagcct tgatagcgtt attaaccagt acttaggtgt ggtcaaacca atgtaccacc
    41341 agtttatcga gtcaactaag cgttatgctg atatcgtcat tcctgaaggg gttagcaata
    41401 ccgtggctat cgacctgttg acgaccaaga ttgcaaagat tttggaagaa gctcgaaaca
    41461 gcaaataatc acatgtggag gccttgcctc ctttttctat ttttccctca taatggtaca
    41521 taaatggaga tttttaggca tatttttggt ataataatac ccataaaaga gcaagtaaat
    41581 gaatagtagg tggagatgga aaagtattta tcggtaacaa ctttgaccaa atatctgaaa
    41641 atgaaattcg ataaagaccc atacttggaa cgggtctatt taactggtca agtttccaac
    41701 tttcgtaaac gacctactca ccaatatttc tccctaaagg atgaccatgc agttattcaa
    41761 gcgaccatct ggtctgggat ttatcagaaa ttagggtttg acctggaaga aggaatgaag
    41821 atcaatgtga ttgggcgtgt acaggtctat gaaccaagtg gtagctactc catcatcatt
    41881 gaaaaagctg agcctgatgg ggttggggcg cttgcgattc agtttgaaca acttaagaaa
    41941 aaattgacag aagaaggcct gtttcaagaa cgcttcaagc aagctctacc ccaattttct
    42001 aagagaattg gggtagtaac cagccgtagt ggagccgtta ttcgagatat tatcacgacc
    42061 gtcagcaggc gattcccagg tgttgacatc cttctttatc ctaccaaggt tcaaggtgaa
    42121 ggggctgcgg aggaaattgc tagaaatatt gcgcgtgcta atcaacggga cgatttggat
    42181 ttgctcatta ttggtcgtgg aggtggttct attgaggatc tctgggcctt taacgaagaa
    42241 attgtggtac gagctatttt tgaatctcgt ttgccagtta tctctagtgt ggggcatgag
    42301 acggatgtga ccttggcaga ttttgtggca gatcgacgcg ctgcaacgcc aacagcggcg
    42361 gctgaactgg caacacctgt gaccaagttg gatgtattag ctcatttgca aaatcaggaa
    42421 aaacggatgg caacggcagt ccgaaatgtt ctatctaaga aacaagaggc tttgaaaaaa
    42481 tgcagtcagt ctgttatctt tagacaacct gagcgcttgt atgacggtta tttgcaacgc
    42541 ttggaccaac tgcaactgcg tttgaaacaa agtttgcgaa ctcggatttc tgataacaaa
    42601 caattagttc aagcaagaac tcatcaatta gtacaattat cacctgttac caaaatccaa
    42661 cgctatcaag accgtttagg acagttggac aagctcttag gtagccaaat ggcgttagtt
    42721 tatgacgcca aggttgctga ggccaagcga ctttcggaag ctttgctcat gttggatact
    42781 agccgaatcg tggcgcgtgg ttatgctatt gtcaaaaaag aagagtccgt tgtagattcg
    42841 gttgagagtt tgaagaaaaa agaccaagta acgcttttga tgcgagatgg tcaagtagaa
    42901 ttagaggtta aagatgtcaa aacaaaagaa atttgaggaa aatctagcag aactggaaac
    42961 cattgtccaa agtttggaaa atggtgaaat tgctctggaa gatgcgatta ctgcctttca
    43021 aaagggcatg gtcttgtcaa aagagctcca agctacgctg gacaaggctg aaaagacctt
    43081 ggtcaaggtc atgcaagaag acggaacaga aagtgatttt gaatgaaaaa gcaagaaaaa
    43141 ttagctcttg tcgagtcggc tttggaagat ttttatggag accagcagtt tgcctctagt
    43201 ttacgggagt ctgttctcta ttctattcat gctggtggca agcgtattcg gccttttctc
    43261 ttgttagaag ttctggaatc cttgcaggtt accatcaaac ctgctcacgc gcaggtagct
    43321 actgccttgg agatgattca tacagggagc ttgattcacg atgaccttcc tgctatggat
    43381 gatgacgatt atcgaagagg gcggttaacc aatcacaaga aattcggtga agctatggcc
    43441 attttggctg gagatgcctt attcttagac ccatatgcct tgattgcgca ggcagatttg
    43501 ccaagtcaga tcaaggtgga cttgattgcc aacttatccc ttgcttcagg tagtctgggt
    43561 atggtggcag ggcaagtttt ggatatggag ggcgaacacc agcacttgtc tctggaagaa
    43621 cttcagacta ttcatgccaa taagactggg aagttactag cctatccctt ccaagcggca
    43681 gctattatag ctgaattgtc acctgaaatg caggtgaagc tgaaaactgt gggtgaattg
    43741 attggacttg cttttcaagt cagagatgat gtactggatg tgacagctag ttttgaggaa
    43801 atcggcaaga cacctcaaaa ggatctgcag gcagaaaaat caacctatcc tgccttgttg
    43861 ggcttggaag agtccattgc cttttgtaac caaaccctgg atcaagctaa tgaaaaatta
    43921 gaagaaattg cccagcagct tccctttgaa acagaatcga ttgtaagtgt agtagaaagt
    43981 ttgagaatca atggctaagg aaagagtgga tgtactagct tataaacagg ggttgtttga
    44041 aacgagagag caggccaagc gaggtgtgat ggctggtcta gtcgtagcag tccttaatgg
    44101 agaacggttt gacaagccag gagagaaaat tccagatgac accgaattaa aactcaaggg
    44161 ggagaaactc aagtatgtca gccgtggtgg tttgaaactg gaaaaggcct tgcaggtctt
    44221 tgatttgtcg gtggatggcg cgactacgat tgatatcggg gcctctactg gaggttttac
    44281 cgatgtcatg ctacagaata gtgccaagtt ggtctttgca gtcgatgttg gtaccaatca
    44341 gttggcttgg aaattacgcc aagacccacg agttgtcagc atggagcagt tcaattttcg
    44401 ctatgctgaa aagactgatt tcgagcagga gccgagcttt gccagtattg atgtgagttt
    44461 catttccctt agtctgattt tgccagcctt gcacagtgtc ttggctgatc aaggtcaggt
    44521 ggtagcactt gtcaaacctc agtttgaggc aggacgtgag cagattggga aaaatggaat
    44581 tattcgagat gctaagattc atcagaatgt ccttgaatct gtaacagcta tggcagtaga
    44641 ggcaggtttt tcagtccttg gcttggactt ttctcccatc caaggtggac atggaaatat
    44701 tgaattttta gcgtatttga aaaaagaaaa gtcagcaagc aatcagattc ttgctgagat
    44761 taaagaagca gtagagaggg cgcatagtca atttaaaaat gaataaaaaa gaaagacttg
    44821 aaaaaattag aagattggtg acagattatc aaatcggcac gcaagaagaa attgtagaac
    44881 atttgaaaga agcaggtatc actgccactc aggcgacggt atcccgagat atcaaagagt
    44941 taggtattgt caaaattcct ttgagagaca acacctatgt ctacgaattg ccaaaatcaa
    45001 tcgtaaaaag tctgcaactg gctgaagaca atatcgaatc ggctgaattg atggataaga
    45061 tgatcaatct ccaagttatt ccaggaaata cggcttttgt aaaagctcag ttaatcgaga
    45121 cttttgcaga caagattttt agctgtttga ctgatgatag ctcgatttta gtcattgcca
    45181 gaagtggaag tctagcagag gaaatctttg aacaagtaaa aaattggtag gtctatatgt
    45241 tacttgaaat ttcgataaaa aactttgcca ttattgaggc tatttccctc aattttgaaa
    45301 aggggatgac tgtcctgact ggtgaaacgg gtgcagggaa gtcgattatc attgatgcca
    45361 tgaatatgat gttgggagct cgtgcgacga cagatgttat tcgtcatggt gcaccaaagg
    45421 cagagattga ggggcttttc tcagttgaga atagtcgtct tttacaggaa atttttgatg
    45481 agcaaggttt agaattaggt gatgaaatta tcatccgtcg ggaaatcttg caaaatggtc
    45541 gtagtatcag ccgtgtaaat ggtcagatgg ttaatctgtc tgttttgcga actattgggc
    45601 aacatcttgt agatattcat ggtcagcatg accatgagga gttaatgcgt ccccaactgc
    45661 atatccagat gttggatgaa tttggtgatg ccgctttttg ggacttgaaa gaaacctatc
    45721 aaacgagttt tgatgcctat cggaaaatgc gcaagcaggt tctggaagtc aagaaaaacc
    45781 aacaggaaca caaggcacgt atcgaaatgt tggaatttca aatggcagag attgaggcag
    45841 caaacttgca ggctggagaa gacttggatc tcaatcaaga gcgagataaa ctcctcaacc
    45901 acaaaaatat tgcggataca ctgaccaatg cctacagtat gttggacaat gaagattttt
    45961 caagtctggc caatgttcgt tcagccatga atgacatgga aagtgtcgaa gagtatgacc
    46021 ctgaataccg tgaaatttca agctctctgt ctgagaccta ctatgtttta gaagacatta
    46081 gcaaacgttt ggaagctatc attgaggacc ttgattttga tggcaatcgc ctcatgcagg
    46141 ttgagaatcg tttggacctc cttcatacta ttactcgtaa gtatggtggg actgttgatg
    46201 atgttttgct ttattttgcc aagattacgg aagaatacaa tctcttgaca ggcaataatc
    46261 tttcgtctga ggacatggaa gcagagctta agaagttgga agtcaatctt gtcaatttgg
    46321 caggtcaact tgcttctgct cgtcataatt tggctcagca actcgaagct gagattaaac
    46381 aagaactgca agatctttat atggaaaaag cccagtttca ggttcgtttt agtaagggaa
    46441 aattcagtcg tgagggaaat aaaatggttg agttttatat ttcaaccaac cctggagaag
    46501 actttaaacc cttggttaag gttgcttctg gaggggaatt atctcgtctc atgttagcca
    46561 ttaagtctgc cttttcacgt aaagaaggca agactagcat tgtctttgat gaggtggata
    46621 cgggagtttc aggtcgtgtt gctcaagcta ttgctcagaa aattcataaa attggtcagc
    46681 atggtcaggt tttggctatc tcccatttgc cacaagtaat tgcgattgca gattatcaat
    46741 tctttattga gaagattagt aatgaccatt caacggtttc gactgttcgt ctcttgacgg
    46801 tcgaagagcg agtggaggaa gttgccaaga tgttggcagg tgatgatgtg acagaagcag
    46861 ccctgacgca agcaagagaa ttgttgagaa acagggagaa ataagatgac agattattat
    46921 gtaattggag atgttcacgg aaaagctggg atgctggaag accttctcaa aacctgggat
    46981 ggtcagcccc agttgctctt tctaggtgat ttgattgaca gaggtgagga tagtcaccgt
    47041 gttcttgaaa tggtcaagga cttggtggac aatcaagggg ctatctgttt gtccggaaac
    47101 cacgagtata tgtttttgac ttggctagat gacccagaag aaagttatga ccattatagt
    47161 cgcaatggtg gagatacaac cattaactct atcctaggtc gtcccttgga tgcaccagtt
    47221 gatggagtag aagatgccaa gcgggttgcc gctgaagcgg cagacttggt cgaattcatt
    47281 cgtcaaatgc catttgtagt agagacagac aagtatatct ttgttcacgc aggtattgat
    47341 ttgaccttgg acgactggca tgaaacaaca gattataaaa aagtatggct tagaaaacca
    47401 ttccacggag ctgaaaatca tactggaaaa accattgtct tcggtcatac accggtctat
    47461 ggtttattac agcaagaacg cggtacatct gagctttgga ctacagaaga tggcaagatt
    47521 ggaatggatg gaggagctgt ttatggtggt gtccttcacg ggattgtctt tacagaccaa
    47581 ggaatgacag aacaccactt tatcgaaaat gacggctttg tcgctgaaga ttagtactcc
    47641 tagcagggta tggtcttgtc aaaatatcaa aaacaattta taataaatag ataccctgaa
    47701 aggaagagaa tcatgaactt agaagaattg aaaaaacgac aggagaagat ccgtaacttc
    47761 tctattatcg cccatattga ccacggaaaa tcaactctag cagaccgcat tttggaaaaa
    47821 acagagacgg tttcaagtcg tgaaatgcag gcccagcttt tggatagcat ggagctagag
    47881 cgggaacgtg gaattactat taagttgaat gccatcgagt tgaattacac tgcaaaagat
    47941 ggggaaactt atattttcca cttgattgac acaccagggc acgttgactt tacctatgaa
    48001 gtttcacgtt cgctagctgc ctgtgagggt gctattttgg tggtcgatgc ggctcaagga
    48061 attgaggctc aaactcttgc caacgtttat ctggctttgg ataatgattt ggaaatcatg
    48121 cctatcatta ataaaattga cctgccggct gcagatccgg agcgcgtgcg tacagagatt
    48181 gaagatgtga ttggtttgga tgccagtgaa gcagttttgg cttctgccaa ggctggtatt
    48241 gggattgaag aaatcctcga gcaaattgta gaaaaagtac cagcaccaac gggtgatgtg
    48301 acggcgccac ttaaggcctt gattttcgac tctgtttacg atgcttaccg tggggttatc
    48361 ctccaagtgc gtgtcatgga cggagtggtc aaacctggtg ataagattca gctcatgagc
    48421 aatagtaaga cctttgatgt ggccgaagtc ggtattttta caccaaaagc ggttggtcgt
    48481 gatttccttg cgactggtga tgttggttac attgcggcgt ctatcaagac ggttcaggat
    48541 actcgtgtgg gtgataccgt taccttggca accaatcctg cggcagaacc attacatggt
    48601 tataagcaga tgaatcctat ggtctttgcg ggtctctacc caatcgaatc aaacaagtac
    48661 aatgacctgc gtgaagcgct tgaaaaattg caactgaatg atgctagtct tcagtttgaa
    48721 ccagaaacat ctcaggcact tggatttggt ttccgttgtg gatttcttgg acttctccat
    48781 atggatgtta tccaggaacg tttagagcgt gagttcaata ttgacctcat catgacagct
    48841 ccgtctgtta tttacaaagt taatttgacc gacggtgagt ctatggatgt gtctaaccca
    48901 tctgagtttc cagacccaac taagattgcg accattgaag agccttatgt caaggcgcaa
    48961 atcatggtac cacaggagtt cgtcggagca gtaatggagc tagctcagcg taagcgtggg
    49021 gactttgtga ctatggacta tattgatgat aaccgtgtca atgttatcta tcaaattcct
    49081 cttgctgaaa ttgtctttga cttctttgat aaacttaagt cttcgacacg tggttatgca
    49141 agctttgact acgaattgtc agaatatcgc ccatctaagc tggtgaaaat ggatattctt
    49201 ctcaatggag ataaggtgga tgccctcagc tttatcgttc acaaggactt tgcctacgaa
    49261 cgtgggaaac tcatcgttga taaactcaag aaaatcatcc ctcgccaaca atttgaagtt
    49321 ccaatccaag cggctattgg acacaagatt gtcgctcgta ctgatatcaa ggcccttcgt
    49381 aagaacgtac ttgctaaatg ttatggtggt gacgtttctc gtaagcgtaa actccttgaa
    49441 aaacaaaaag ctggtaagaa acgcatgaaa tccatcggat cagttgaagt tccgcaagaa
    49501 gccttcctca gcgtcttgag tatggatgaa gaataagtta tcagaaatgc ctttccttgt
    49561 tcacaggaaa tttataagaa tcaatagtaa agcaggctta gatggtctgc ttttttagtt
    49621 tttatactct tcgaaaatct cttcaaacca cgtcagcgtc gccttaccgt atgtatggtt
    49681 actgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga cttcgtcagt
    49741 ttcatctaca acctcaaaac tgtgttttga acaacctgcg gctagcttcc tagtttgctc
    49801 tttgattttc attgagtatt agaacagaaa aaatgcttgg agtatttgtt tgtgtgttta
    49861 cttttatata acaaactata aacacaacaa aaatataaaa aaagagacaa aaaagaacag
    49921 aaagtaatta acaacgattt catatagtgt tatacttata gcataaagtt aatggaaaga
    49981 agggaaaacc ttatggtatt agattatttc tttgacaaaa accttgtgtt ttgcttagaa
    50041 gcggataatc aagaataact ctttgatcag gtagcaagct tgttggagga acgagaaatt
    50101 gt
//
LOCUS       DAVBCB010000007        48474 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVBCB010000007 DAVBCB010000000
VERSION     DAVBCB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 48474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.007"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(308..1474)
                     /gene="recA"
                     /locus_tag="VQU28_000392"
     CDS             complement(308..1474)
                     /gene="recA"
                     /locus_tag="VQU28_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HET2475812.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVPLDLGDELEIEIEE"
     gene            complement(1529..2785)
                     /locus_tag="VQU28_000393"
     CDS             complement(1529..2785)
                     /locus_tag="VQU28_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HET2475813.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNLLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQVDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGISSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(2869..3885)
                     /locus_tag="VQU28_000394"
     CDS             complement(2869..3885)
                     /locus_tag="VQU28_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2475814.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNVERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEKALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(3893..4411)
                     /locus_tag="VQU28_000395"
     CDS             complement(3893..4411)
                     /locus_tag="VQU28_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2475815.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(4401..4844)
                     /gene="tsaE"
                     /locus_tag="VQU28_000396"
     CDS             complement(4401..4844)
                     /gene="tsaE"
                     /locus_tag="VQU28_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HET2475816.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(4930..5550)
                     /locus_tag="VQU28_000397"
     CDS             complement(4930..5550)
                     /locus_tag="VQU28_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475817.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(5933..6796)
                     /locus_tag="VQU28_000398"
                     /pseudo
     CDS             complement(5933..6796)
                     /locus_tag="VQU28_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            6961..7155
                     /locus_tag="VQU28_000399"
     CDS             6961..7155
                     /locus_tag="VQU28_000399"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475818.1"
                     /translation="MKKKRVSRMKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQ
                     LEIRVQSQKKSNGLDVGKAD"
     gene            7470..7766
                     /locus_tag="VQU28_000400"
     CDS             7470..7766
                     /locus_tag="VQU28_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HET2475819.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            7869..9884
                     /locus_tag="VQU28_000401"
     CDS             7869..9884
                     /locus_tag="VQU28_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2475820.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYPNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFIALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAGLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSMLVLKGG"
     gene            9886..10518
                     /locus_tag="VQU28_000402"
     CDS             9886..10518
                     /locus_tag="VQU28_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475821.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLTYLDLDKRIFELSGGESQRVALAKVILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDRLK"
     gene            10659..10937
                     /locus_tag="VQU28_000403"
     CDS             10659..10937
                     /locus_tag="VQU28_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HET2475822.1"
                     /translation="MKKFDNYIVEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(11446..11859)
                     /gene="ndk"
                     /locus_tag="VQU28_000404"
     CDS             complement(11446..11859)
                     /gene="ndk"
                     /locus_tag="VQU28_000404"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HET2475823.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(11970..15647)
                     /gene="rpoC"
                     /locus_tag="VQU28_000405"
     CDS             complement(11970..15647)
                     /gene="rpoC"
                     /locus_tag="VQU28_000405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HET2475824.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGEVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPYAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(15680..19291)
                     /gene="rpoB"
                     /locus_tag="VQU28_000406"
     CDS             complement(15680..19291)
                     /gene="rpoB"
                     /locus_tag="VQU28_000406"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2475825.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSSLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            19707..19777
                     /locus_tag="VQU28_000407"
     tRNA            19707..19777
                     /locus_tag="VQU28_000407"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19739..19741,aa:Cys,seq:gca)
     gene            complement(19975..21306)
                     /locus_tag="VQU28_000408"
     CDS             complement(19975..21306)
                     /locus_tag="VQU28_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HET2475826.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(21597..22421)
                     /gene="endA"
                     /locus_tag="VQU28_000409"
     CDS             complement(21597..22421)
                     /gene="endA"
                     /locus_tag="VQU28_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HET2475827.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(22460..22648)
                     /locus_tag="VQU28_000410"
     CDS             complement(22460..22648)
                     /locus_tag="VQU28_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HET2475828.1"
                     /translation="MNKKSSYVVKRLLLVIIVLILGTLALGIGLMVGYGILGKGQDSW
                     AILSPAKWQELIHKFTGN"
     gene            complement(22641..23924)
                     /gene="murA"
                     /locus_tag="VQU28_000411"
     CDS             complement(22641..23924)
                     /gene="murA"
                     /locus_tag="VQU28_000411"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2475829.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(23948..24178)
                     /locus_tag="VQU28_000412"
     CDS             complement(23948..24178)
                     /locus_tag="VQU28_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HET2475830.1"
                     /translation="MVQLLFTLSSHMLFIYVSFYLLKDLVRWEKVLKVTAENTRKVRV
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(24236..25273)
                     /locus_tag="VQU28_000413"
     CDS             complement(24236..25273)
                     /locus_tag="VQU28_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HET2475831.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(25257..25745)
                     /gene="coaD"
                     /locus_tag="VQU28_000414"
     CDS             complement(25257..25745)
                     /gene="coaD"
                     /locus_tag="VQU28_000414"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HET2475832.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(25735..26274)
                     /gene="rsmD"
                     /locus_tag="VQU28_000415"
     CDS             complement(25735..26274)
                     /gene="rsmD"
                     /locus_tag="VQU28_000415"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HET2475833.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKTVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(26339..27331)
                     /locus_tag="VQU28_000416"
     CDS             complement(26339..27331)
                     /locus_tag="VQU28_000416"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HET2475834.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(27443..28126)
                     /locus_tag="VQU28_000417"
     CDS             complement(27443..28126)
                     /locus_tag="VQU28_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HET2475835.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(28182..28922)
                     /locus_tag="VQU28_000418"
     CDS             complement(28182..28922)
                     /locus_tag="VQU28_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HET2475836.1"
                     /translation="MTIITSKANSVVKNAKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFALENYRNQLAAFPQTVWISEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            28956..29234
                     /locus_tag="VQU28_000419"
     CDS             28956..29234
                     /locus_tag="VQU28_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HET2475837.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            29312..30238
                     /gene="yidC"
                     /locus_tag="VQU28_000420"
     CDS             29312..30238
                     /gene="yidC"
                     /locus_tag="VQU28_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2475838.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(30313..31107)
                     /gene="pflA"
                     /locus_tag="VQU28_000421"
     CDS             complement(30313..31107)
                     /gene="pflA"
                     /locus_tag="VQU28_000421"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HET2475839.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(31233..32483)
                     /locus_tag="VQU28_000422"
     CDS             complement(31233..32483)
                     /locus_tag="VQU28_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HET2475840.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(32550..33377)
                     /gene="purR"
                     /locus_tag="VQU28_000423"
     CDS             complement(32550..33377)
                     /gene="purR"
                     /locus_tag="VQU28_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HET2475841.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLHEFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(33644..34585)
                     /locus_tag="VQU28_000424"
     CDS             complement(33644..34585)
                     /locus_tag="VQU28_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HET2475842.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(34575..35831)
                     /locus_tag="VQU28_000425"
     CDS             complement(34575..35831)
                     /locus_tag="VQU28_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HET2475843.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(35833..36495)
                     /locus_tag="VQU28_000426"
     CDS             complement(35833..36495)
                     /locus_tag="VQU28_000426"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HET2475844.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDGFVGVDRGSLWILE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQYFVQARPEKDDTDLELALLTIFKQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLASYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(36458..37114)
                     /locus_tag="VQU28_000427"
     CDS             complement(36458..37114)
                     /locus_tag="VQU28_000427"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HET2475845.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDGHFVP
                     QISFGAGVVEALRPHSKMVFDCHLMVSNPEHHLEDFVRAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTPVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALRDEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            complement(37125..38003)
                     /gene="rsgA"
                     /locus_tag="VQU28_000428"
     CDS             complement(37125..38003)
                     /gene="rsgA"
                     /locus_tag="VQU28_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HET2475846.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDGGELDFYQQTYGDIGYDFVTSKEELLPLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     TAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGTIAPFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(38596..39369)
                     /locus_tag="VQU28_000429"
     CDS             complement(38596..39369)
                     /locus_tag="VQU28_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HET2475847.1"
                     /translation="MRKKLVNSLMCAVVLSGFPIATYANEKNIGPNITVSNGQTRLSI
                     PEKNGVEVIQSEENRVVYEVKEAAKIETSDGDMVAPIVVDSRITSINNQVVQGKTVYT
                     ADLEKATFVEEGYNKLAKPTNILDHIFGIQKVHAYTQNITTHQSQFDSTYSVELYTVV
                     HWVSYDGGVQKNITKISGGVDFHERDIEIESSSVEVRQGGWYSSQQKWFHPGARSSWE
                     YSTGFSKVGPGENRVVTNYTVNLIRYGDRWSAYLQNNPW"
     gene            complement(40059..40931)
                     /gene="rsmA"
                     /locus_tag="VQU28_000430"
     CDS             complement(40059..40931)
                     /gene="rsmA"
                     /locus_tag="VQU28_000430"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HET2475848.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAEVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPVPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLE
                     EFASLADALKGQGL"
     gene            complement(41182..41400)
                     /locus_tag="VQU28_000431"
     CDS             complement(41182..41400)
                     /locus_tag="VQU28_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475849.1"
                     /translation="MENMTNPLLLVQIRVHGYLLDFASSEGQRQKHYRLKNLPQTVEL
                     TVDDVEEDVDLTLPENRSYQEADFFDTQ"
     gene            complement(41468..42109)
                     /locus_tag="VQU28_000432"
     CDS             complement(41468..42109)
                     /locus_tag="VQU28_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2475850.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(42111..44141)
                     /locus_tag="VQU28_000433"
     CDS             complement(42111..44141)
                     /locus_tag="VQU28_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HET2475851.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEVLSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLAATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQFICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSTYYQRLFTDYL
                     QNFSSESVEVTSQKYYLPQVSLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(44410..45309)
                     /locus_tag="VQU28_000434"
     CDS             complement(44410..45309)
                     /locus_tag="VQU28_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2475852.1"
                     /translation="MLIGQKIKEIRIEKGVSRPYFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMSQIIEKEVYSTNDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALREERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(45381..45941)
                     /gene="rnmV"
                     /locus_tag="VQU28_000435"
     CDS             complement(45381..45941)
                     /gene="rnmV"
                     /locus_tag="VQU28_000435"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HET2475853.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINAQDIERI
                     QRLHQRQGVIVFTDPDFNGERIRRMIMTAIPTVRHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(45941..46714)
                     /locus_tag="VQU28_000436"
     CDS             complement(45941..46714)
                     /locus_tag="VQU28_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HET2475854.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVTQMNIVGFDKPTIE
                     RALELVDEYDQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKVTDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(47019..47912)
                     /locus_tag="VQU28_000437"
     CDS             complement(47019..47912)
                     /locus_tag="VQU28_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HET2475855.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIVMVTVPKRPVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(48196..48330)
                     /gene="rpmH"
                     /locus_tag="VQU28_000438"
     CDS             complement(48196..48330)
                     /gene="rpmH"
                     /locus_tag="VQU28_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HET2475856.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
BASE COUNT        13990 a        10915 c         9042 g        14527 t
ORIGIN      
        1 taaagctatt atatcagctt tccagaggag cgacaagaaa cagcaaactt atatatagcg
       61 gaatgaaata aggaaagtca aatcgatttt taacaatgtt ttagaagtag atatgtacta
      121 ttctagtttc gatctattat agttggttga gtttggaata gtacgctgta gctgctaaaa
      181 catttctaga aattaatttg actttcctaa tagagttgtt catatcttat ttcaagctac
      241 tatatataat ctaaaaactt gaatcaaaaa tcgaaaaagt agcggatttc tccactgctt
      301 taacagctta ttcttcaatt tcgatttcaa gttcatcgcc taagtcaagc ggaacttctt
      361 cattcactgc ttcttctttc tttggctcat cttttttgtt ttcagtatct tgttctgaaa
      421 cttcttctcc atcaatcaag ccaaatttag aacggacttg cttatcaatt tcatcaaaga
      481 tttctgggtg ctctgccaag tatttcttag cattctcaga accttgccca attttttcat
      541 ctttgtaaga ataccaagcc cctgcttttt tgataatatc caaatcgctt gcaatcttca
      601 aaagctcacc agtcttagaa attccttctc cgtacataat ttcaactacg gcttccttaa
      661 acggtggagc taccttattt tttacaacct taatcttagt ttctttaccg acattggttt
      721 ctttttggtc accagttccc ttaatttgtg tattaccacg aacatccaag cggactgaag
      781 catagaattt caaagcacgt ccgcccggtg ttgtttctgg atttccaaac atcactccaa
      841 ctttttcacg caattggttg ataaaaatgg caattgtttt ggttttattg atagaggcgc
      901 caagtttacg catggcctgg ctcatcatac gagcctgcaa accaacatgg ctatctccga
      961 tatctccatc aatttccgca cgaggaacaa gggcagcaac tgagtcgact acgacaagat
     1021 caactgcacc tgagtcaatc aattttcccg caatctcaag accttgctct cctgagtctg
     1081 gttgagacaa gagcaattcg tcaatattga caccaagggc cgcagcataa gctggatcaa
     1141 gggcatgttc cgcatcgata aaagcagcaa tcccaccttc tttttgcgct tgtgcaactg
     1201 catgaagggc aaccgttgtc ttaccagatg actctgggcc atagatttcg atgatacgtc
     1261 ccttaggata accacctgag ccaagggcaa tgtcaagagc taaagaacct gagctcatca
     1321 cttgcacctt ttgctccgca cgttcaccca aacgcatgat tgatccttta ccaaagtctt
     1381 tctcaatcaa tttaagagcg tcattcaagg ccttttcacg ttctgcccca aatttttttg
     1441 aaatttcttc taattttttt ggttttttcg ccattctatt ctcctacatt ctaatgatcc
     1501 tcagacctat ctactattat atcaaaaatt agtcacttaa taaagccttg cgaactaggt
     1561 taaaggcatg cataaccgca atgtgacgta catctgctcg gcttctgcct ccaatattaa
     1621 ccttgataac ctcagttcct tgatcttgcg ccaagcctat gaagactgtc ccaactgggt
     1681 gtccttctag gctatctggt cctgccactc cagtcaaact aattccaaaa tcagactggg
     1741 tcttgcttcg tgcctgctca gccatcttct gagctgtaaa ttcagacacc acaccatgtt
     1801 cttccaaatt cttggcagga atatccaaca tccttgattt ttcctccaag ctataggtca
     1861 caaaaccacc cttaaatata cttgaaattc cagaaaaatt cgccacggta gcttggaaaa
     1921 gacctgccgt caaactctct gcagccgcga tggttttccc ttgccttttc agttcttcta
     1981 ccacaatgct ggctaaacta gtttcttccc cataaccata gcaaaagtct cgtaaagaaa
     2041 ttccttcgaa agtctggcag tccaagattt gattttccaa gatatccagc gcttgattcg
     2101 cctcttcttg actgctagcc tttgttgaca gacgtagagt gacttctcct gtcttggcat
     2161 aaggggccaa ggtaggatcg acctgattat caattaaatc agccaaaatc gtaaccagct
     2221 ggctctcgcc aatcccaaag aaacgaagaa ctcgggaata cagcttgctc cctgtcatca
     2281 acttgggtag aagtaggttt aagaccatgg gtttcaattc acttggcgga cctggaagga
     2341 cgacataggt cactccgtct acttctaatt ttcctccaac agccagtcct gtttcgtttg
     2401 gcagtggaat cgctccttct acaatttgag cttgtctttc gttattcggt gttcgggcat
     2461 agtctggtcg cagggcaaaa aagatatcca acttctcctg agcctgagga tcaaagacta
     2521 atgctttccc taaaaattta gctagggttt gtttggttag gtcgtcctca gttggcccca
     2581 aaccgcctgt caaaatcacc agactgctac gttgactggc aatctcaagc aaagacaaga
     2641 gacgaacttc attgtctcct acagccgtct gaaaatatac atctacccca atctcagcta
     2701 gtttttccga caaaaactgg gcattggtgt tgacaatctg tcctgtcaaa atctctgttc
     2761 caacagcaat gatttctgct ttcatgtttc ctcctaccta tctattcgta tttttttgaa
     2821 aaaatcgcag gaattttcct acgattgatt ttttatttgt atcaaaagtt aattatcttc
     2881 atcaccaaca ggtgctctgc caaataaatc ttcaaataaa accgcattgg tttcaagctg
     2941 agtaacttct tcttgtccca aagaacgtcg gagtatattt tgcatttcca acatatgtgc
     3001 tcttgaaaca atctggtaag aaacaccttg aagtatctct ccttcaccct gcaactgctg
     3061 agtttcaatg gttttaaatg aatctttata gcctagcaag ttagggatac tttttgcaga
     3121 caaatcaata ttggtctgca tattgtcact caaagctttt agaatctctt gataatgacc
     3181 aacgctattt aaactgagag ctttttccat gactttttga ataacttcac gttgacgttt
     3241 ttgacgacca taatccccct caggatcttg gtaacgcatt cgtgcataga ctagggcttt
     3301 ttctccccca atatgttgct ccccaacacc gatagaaata gtattaaatt cttcttggtc
     3361 actgatagaa attgggaaac ctaggatatt attgactgta atacctccta ctgcatccac
     3421 tagtttttgc aaccctctca tattgaccat cacatagcga tcaatatgga tattcatcat
     3481 tttttgaatg gtttctatag caagctctgc tccaccatct gcatatgctg agttcagttt
     3541 cgcttcatga gcctgaccat tccctgattc aatgcgcgtc agaatatccc gctctaaact
     3601 catcattgtt gtttttttcg ttttaggatt cactgtcatc aagatcatgc tatcacttct
     3661 accgacccaa gtttcagttc gttcaacatt tccggtgtcc actcccatta acagaatggt
     3721 tagaggttca gtcgcttcaa taaccttggt ttcttcgccg atttttttat aggttttagc
     3781 taaggtttct gtcccttgtt gataaatagt ataagcaaaa acacctactc ctactacagt
     3841 tacagaaagt aaagctagca ccattccaat aattttttta accatatttc tactaaccta
     3901 tcagtttacc catcaagtaa acatcgataa atttcccttc ttctatatat gccccacgct
     3961 cttggctacc ttcaatgaca aagccatgct tttgataaag atggactgct gcttgattac
     4021 gagtttggac agtcagttgg agacgacgca gaatgccact tgcttgtgcc cactctatcg
     4081 cttcttctag caacaaactt cccaagccat tattccaata tctttttcca atcacaatga
     4141 agagatctcc aatatgacgg actctcttac gctgatcagc tgtaatattt acaataccag
     4201 caattttgcc atttaagaat gcaagtaagg ttatctgatt gtccgaacta gcttgcttgt
     4261 tgaggaatat ttccatctcc tcactagtca agagaatacc atctccgtct aggctggtaa
     4321 agtctgtctc caaactcaca cgatttaaaa aggccactaa ttcagctgca tctttgggct
     4381 ctgcttccct aatgagcaat tcatactcca tattgaagct cctctaacaa tttctcagca
     4441 cgcaaaccct ttgcctgaaa atttaaacgg cgtccatctg cttcttttag aatttccaat
     4501 tctaaataag catctggcaa ggcatctcct aagagatttc cccactcaat aacagtcacg
     4561 ccgccaccaa agataaactc atccaagtcg atagaatcag catctccttc aatacgataa
     4621 acatctaggt gataaagtgg aagtcgacct tcatactctc tcacgatagt ataggtggga
     4681 cttttaatca tttgagaaat ctgtaatcct tttgcaagtc ctttagtaaa ggtcgtttta
     4741 cctgcaccca gttctccagt taagattaaa acatcattct ttgctaatag atggcccaaa
     4801 cgctccccta aggcttgcaa ctcttcttca ttttttgtgt acatactctt attataccaa
     4861 aaacttttct tttgtgtcta ttttcctact aaacttatca tcataacatc cataaaaaac
     4921 aggctttctc taaaagaaaa tgagcgtaac aatgaccaat acaagatctc ggaaaatatg
     4981 accataaaag gaaacttcct tcttaaccga atttgggaca agataggctg caaaaaacaa
     5041 gcccagtcca atataaatca gaagtgagac aatggtcatt ggatttctta agaaaagaag
     5101 tgttgctaaa atagtcacca acactgtctt ttttctgtct agcatagcaa gaaaatcgcg
     5161 cacgtatttt ttcaagggta aaaaaatcag caaatctagc ccaaatagga aaaagaagga
     5221 tggcaataaa aagtcaacta attcttgctg cagcgtattt ttgatgaaca agttatctga
     5281 caaaacaaga acagctccta acaaattaat taagagtaac atactgtaaa aaagcttcac
     5341 cgacttctta ctggctagga cactatggac ttcttgctta cgggtataaa gataatttac
     5401 tccagcacag attcctgaaa cgaaaaccat gctcccgatg aaaaaagctg tactttgttt
     5461 aaaggacaag atgcattcct tccataggaa acagctactc aaactgattt gaattaaagc
     5521 taacaaaaat aagattctca ttgatttcat cttctctctc ccttcctacc aatcattata
     5581 ctaggagaaa agagagaact gtttctaatc ttctcaaatg tctctttaag acgctaaaca
     5641 aacactagag actaatactc aatgaaaatc aaagagcaaa ctaggtagct agccacaggt
     5701 tgctcaaaac actgttttga gattgcagat agagctgacg tgatttgaag agattttcga
     5761 agaatataaa tttgaaatca tgaaaatccg tcaaacgggt ggttgttttg tctcgcacct
     5821 cacggagcga gacggactca gagtcacata attataaggc tgatagtatt aatctaacta
     5881 tcagccttac aggttattta acgtttcaga aaaactataa tgtcaagatt aactaaacag
     5941 tatctaattc cttcaaataa ttttctatct tcatcagcat taaaggattg ttataaatct
     6001 tgcatagctc tcttgcttct atataataat ttttgacttg ttctctgtct agaaatttgg
     6061 ctccagcatt tcctacaaga ataagtagag gagccaattg gtagcttgtc tgtctttgtt
     6121 tacagagttc gatagtctca agagcttctt ggatggcttc attatatttt tcctttgata
     6181 ctaggtagtg agcgtaattg taacgaactc tgatgtagcc aaataaaaac tcttgatgct
     6241 caaaattttt tgtctgatat aactccatca aatgagagta gtttacctca tattcttgtt
     6301 cacgacccac taaggaatag aaattagata gagtattcaa cgcctttaaa taaatcaaag
     6361 tatttgagga gacttttaat aatatatttt ccaatgaaga aattgcttca cacttactgt
     6421 catattgaca gaagtcaata atagctttaa tccattcaag gtaagttcgg tcttctagtg
     6481 ttagaaaagt acttcgttca atctctattc tataaatata ttccaaattt tcataatttc
     6541 tatcatctaa taggcgagca gataattgct tgaaattaga gaggttagat ttaatttcaa
     6601 tttgttcatt aaaaaaataa tctaatggca cttcgagtcg ttgtgagagt ttgaacaaaa
     6661 ggtctgcgga gggaatgaaa taccctctct caattttact aatctggctc tattcacaaa
     6721 ttccttctgc aagagtttgt tgggagagtc tcaactcttt tcttttcaat ctgaatttct
     6781 ctgcgagtgt attcattaaa gataataaac cttcctattt gcttaatttc attataaaat
     6841 ttttagttca aaatagcaag ttaaaggaat aaataattct ttgcggtaat aaacaaaaaa
     6901 aagtcaaaaa ttacataaaa atgcttggct aaataaaata tatttgttaa aataaggata
     6961 atgaaaaaga aaagggtttc acgaatgaag aaaaatcgtg gaattcaaaa attagctata
     7021 ttagtattac taggtgtttt tatgtttagt aacacaattc cttaccaaca gtttattcag
     7081 aagaatagac aattggagat tcgagtccaa tcgcaaaaga agtccaatgg tcttgatgtt
     7141 gggaaggctg attgatttta tattacttta aaattcaact atttagagaa aggtattctg
     7201 atgagagctt gtaacaatac cagatagata taagaatgga gatgtttgat aaaatgcttt
     7261 catagctaac caactgttga aaattatgag ttacaaaatc taaagtattt aagattggtt
     7321 tataaaatta ggtctctgcc tgtagaaagg aacgtgcagt tagaaaggag gaaaaaatag
     7381 gatactataa ttcagactga gatttctaat taaaatatat agttgcattt cgctcatttt
     7441 cataatattt cattcagagg agtaataata tgttaaaatt tagaaaagta gttgcatcat
     7501 cccttgccgt tttggcattt agtttggctc ttcctgtttt ggcagtttct caccgtggtg
     7561 gcgagtggac ctatggaggc catcatgatc caaataattg gggagcattt tcgaattatt
     7621 atcatggttc acagtatcac tgggcctatg ttggaagtaa tggacgtaac aatcaaaaaa
     7681 cagtttatgc aggagcacgt agtgcggcct atgcgtttgt taatacaaat tttggggagc
     7741 aagttacatt tgatgcggga tggtaaagaa taattgaaga agattctttt atcaatagtt
     7801 cagtaagctt gttagtttat agagggcagg ggtatttctg ccctctgttt tagaataagg
     7861 aatgcactat gaaaaaatta tttatactca tatccaacct gcttgctagt ttgttctttg
     7921 tttgggtatt taccatttgg actgatacat atgtctccta ttattacccc aacgttgttg
     7981 tacatgattc ttcccctgaa acaacttttc agcatgttgc tacacgcttg gaaaaactag
     8041 cagaagaaac agatagcttt attgcgattc aacaccaaga tcctaactca gaaggtacta
     8101 cagttttttc ttatacgacc tttggggacg gaaagttgcc tgatgggctt caggaaaaaa
     8161 aactagaaga tgctcaaagt agtagtgttg aaacaaacta ttttgtattc gatggacacc
     8221 tagatattca cttgctaagg gaggagctaa gtcaacttgg cttgactaac atgaacctga
     8281 caattccatc taaactatct acgttaatgg ctatctttag taacggattt cagttaatca
     8341 gtctattgat tttcatctta acctttatag cactgacttt aattagtcaa attagccaat
     8401 tacggtcgtc gggcattcgc ctcatttcag gagagaaacg gtggtccatt ttccttagac
     8461 cagttggtga ggatttgaaa gggatagctg ttggtttcag tttagctggc gtgctcgcca
     8521 ttcttatgca aaaaattctg tcgcttccaa cgcaatccct aatgaccata ggagcaggct
     8581 tgctcagtta taatctcatc ttgttgtcga tctccctgtt tttcgctcaa ctcttcgcag
     8641 ttgggataaa aaagatacac ctgatgcaga ttataaaagg gcaagtgcct gtcagaggaa
     8701 tcattagttt gattttaatc ggtcaactat tagcgattat tattgtaacg ctgggaatag
     8761 ggagtagctt aaagtattcc caagcctggc aacagcatcg gattggacaa gaggcttgga
     8821 gtcaggaaag gcaactgatt accctatcaa tcagccgtga aggaacgagt cctggttttg
     8881 atgaacaagc tcaaaggaaa ctcagaactt ggtatcaatt gatggatctg gctgtttcag
     8941 aacaaaaggc tttcttatct agacaccagt taattgaccg tactttgcaa aatggcatgg
     9001 cttcctccaa aaacttgata acctctacag aatggcacga ttacaacccg aatggcaatg
     9061 tccttatcgt cacaccgcaa tacttggagc gtcaaaacat tcctgtagat acaactattg
     9121 agcaaaagat gaatcacctt gatgtagggg agtttgtctt attgctgcct gaacacctcc
     9181 gttcagagga agaacattat aaatctgttt ttgaagacga cttaaccagt cgcatgtcta
     9241 gtcaagatga acgacagcaa atgactgcta cggtaggtta tttagaatca ggtcaggatc
     9301 gttttgtgta taatacgacc cctatttctt accagcagtt tttgaaagat ccaatcatca
     9361 ttgttataac accccaatca actggtccac agtccatttt gttttggata gacgcagtac
     9421 agaactacgt tctctttaat caattgtctg atgcccagga gcttatccag agacaaggca
     9481 ttgaaaattg ggtctcagaa atgcaaacag gttaccacaa ctacatcaca ttattggata
     9541 atattcagag ggaacgttgg gtaatgctag caggagctgg gcttgggatt gcaacttcaa
     9601 tcttgttgtt taacactatg aataggctct actttgaaga atttagacgt gccattttta
     9661 tcaaacgcat tgcaggtctc aggttcttag aaatccatcg cacttatctc tttgctcaac
     9721 tgggtgtgtt tttactggga tttgttgcga gtgtatttct tcaggtagag ataggagttg
     9781 ctttcttagt cttgttactc tttactggtc tatctctttt acagttacat gtccaaatgc
     9841 agaaagaaaa caagatgtcc atgcttgttt tgaagggagg ttaatatgat tgaacttaaa
     9901 caggtgagta aatcttttgg agaacgagag ttattttcga atctttcaat gacatttgag
     9961 gctggaaaag tctatgcctt aattggttca agtggtagcg gaaaaacaac cttgatgaac
    10021 atgattggga aattagaacc ttatgatggg acgatttttt accgaggtaa agacttggcc
    10081 aattataaat cgagtgattt tttccgtcac gaattgggct acctcttcca gaactttggc
    10141 ttaattgaaa accaaagtat tgaagaaaac cttaagctag gtctcattgg tcaaaagttg
    10201 agtcggtcgg aacagcggtt gaggcagaag caggctttag aacaggtcgg cctgacttat
    10261 cttgacctag ataagcgcat ctttgagtta tcgggcggag aatcgcaacg ggttgccttg
    10321 gcaaaagtta tcttaaagaa tccacccttt attctggcag atgagccaac agcttcaata
    10381 gacccagcaa cttctcagtt gattatggag attctgctat ctcttcgaga tgataatagg
    10441 ctaatcatta tcgcaacaca taatccagca atttgggaga tggctgatga agtgttcacg
    10501 atggatcgtc tgaaataaaa atccttgttt ttaattgcac gatgagttac tgaaatatta
    10561 tcatgaatca ataattggag ttaatttaga attgtacttt attaatattg aggtaacttt
    10621 ttcttgataa aggaagaaat agtggagagg aagttagaat gaaaaaattc gacaattata
    10681 ttgttgagaa gccttgcgat tctaattcag ataaactgca aaaaatctta ataattgaaa
    10741 atttggtaga tgatattttg caatttcctc tcagaatcaa taatagtgta ggagagattt
    10801 tcctcctaca accgtttcaa aagaaaacta tctttattcc atgttatttt gaggaagata
    10861 ttgtgaaagt caaagatgat gataaagttg agtggaattt gttagaattt caaaaattta
    10921 gagcattttt ggcttagtaa tctgtgttga aggctcaaaa cctatggtaa aaaagtcgct
    10981 ttgaaaacgt attgcctcca aagatttagt taaataatga tttaacacaa aaagaaatta
    11041 ttgaagttct ggaaagatgt tgtttcagta ttgagaaaag gtgggaaaaa cttgcgattt
    11101 tcacagagaa aggaagaaaa agtatagaaa tatagtcaat tgaaacaaga acaggacaaa
    11161 agaacctttt gtgccatatt tttctccttt cgctttacaa ttggcttgaa cacctttatt
    11221 gtatcgcgtt tggagttttt ttggtataac cttcgacgcg cacccgcata gcgggtgttt
    11281 ttttgtctcg cacctaacgg agcgagacga actaatagtc acttaatcaa aaaacgcacc
    11341 atatcaaaaa ctaaaaagtt tgatatcatg cgtcatgtct taaactaatt gactatactt
    11401 tctattcaaa tgagctttta accaattgat tgagccaatc cactcttaaa accaaagagc
    11461 aatttctcgc ttagctgact cttctgaatc tgaaccatgt acaacatttt ggataacctc
    11521 attttctcca gcagcttttg caaaatcacc tcgaatagtg cctggtaaag cttcttctgg
    11581 acgagttgca cccatcatgg tccgccaagt ttcgattact ttgggaccag aaatgacacc
    11641 cacaagaact ggacctgaag tcatgaattc acgaatcggt gggtaaaaac tctgaccaac
    11701 caagtcctga tagtgctggt caatcaactc ttctgaaact tgtgaacgaa actccaattt
    11761 ttcgattgta aatccacgtt gttcgatgcg ctttaacact tcacccacta gccctctttt
    11821 tacaccatct ggtttgatga taaagaatgt ttgttccata cccgtctcct ttgtcagctt
    11881 ctttctttta ttttaccaca tttcgtggaa aaatggagaa agttttcaga atagagaatg
    11941 agagaacccg agggttctct cattctctat tattctactg tttcttccac agtttcaacg
    12001 gcagtatcca caactacttc tgttgtttct tcatttcctt cttcctctac tggaggatta
    12061 aggtattctt cttcgttgac agcatatggt tcaaggttac ggtaacgggc cataccagta
    12121 ccagctggga tgatcttacc gatgataaca ttttctttaa gtccaaggag atggtctttc
    12181 ttaccacgga tagctgcgtc agtaaggaca cgagttgttt cctggaagga agccgctgac
    12241 aagaaactgt ttgtttcaag tgaggctttg gtaattccca taaggactgg gcgacctgtc
    12301 gctggaactc cacctgcgat aaggacatct ttgttggcat ctgtaaagtc attgatatcc
    12361 atgagggtac ccatgagaag atctgtatca cctggatcca tgacacggac tttacggatc
    12421 atttgacgaa ccattacctc gatgtgtttg tcaccgattt ctaccccttg gctacggtaa
    12481 actttttgta cttcaccgag aaggtacgtt tcaactgaca agacatcacg aactgcaagg
    12541 agacgttttg gttggataga accttctgtc agagcagcac cacgcgctac ttggccccca
    12601 acttcgacac gcatacgagc tgtaaatgga acgacatatt caccttcgcc agtttcaccc
    12661 ttaacaaaga ctttcttggt acgagttgat gcatcttctt cgatagcagt aacttctcct
    12721 ttaacctctg tgataaccgc ttccccttta ggattgcggg cttcaaagat ttcttggaca
    12781 cgaggaagac cctgagtgat atcggtattt gaggcaaccc cacctgtgtg gaaggtacgc
    12841 attgtaagct gtgtaccagg ttccccgata gattgggcag caatggtacc aactgcttca
    12901 ccaacttcaa ccgcatcacc agtcgccaag ttgataccgt aacagtgacg gcagacaccg
    12961 tgacgagtgt tacatgtaaa tacagaacgg atagtcactt cttccacacc agcattgaca
    13021 atttcacgcg ccttgtcttc tgtaatcaat tcatttggac caataatcac tgcaccagtt
    13081 tctggatgtt taacagtttt cttagtgtaa cgaccgttga gacgctcttc gagagactcg
    13141 atcatctctt ttccttctgc gatagaacgg atcaagagac cacggtcagt tccacagtcg
    13201 tcctcacgga tgataacgtc ttgggcaacg tcgaccaaac gacgagtcaa gtaacctgag
    13261 tcggctgtct taagggccgt atcggtcata cctttacgag caccgtgagt tgagaagaac
    13321 atttccaata ccgacaaacc ttcgcggaag tttgaaagga ttggcaattc catgatacgt
    13381 ccattcggag cagccatcag accacgcata ccggcaagct gtgagaagtt tgagatgtta
    13441 ccacgggctc cagagtccat catcataacg attgggttct taggatcttg gttagcaatc
    13501 aagcgtttct caagtttttc acgggcagca cgccattcag ctgtaacagc attgtaacgc
    13561 tcgtcgtctg tgatcatacc acgacggaat tgtttggtga tttgctcgac acgtttgtgt
    13621 gattcttcaa tgatttcagc cttgtcatca acgactggga tatcggcaat acccactgtc
    13681 aatcctgcaa gagttgagtg gtggtaaccg agattcttca tgcggtcaag tagggcagaa
    13741 gtttctgtcg tacggaaacg tttgaagatt tcagcgatga tatttccgag gtttttcttc
    13801 ttgaacggag ggttgagctc aagattgctg atagcttcct tgatatctcc accaagtggc
    13861 aagaagtatt tagctggaac accttctgtc aagttggcat tgtttggttc ttgcaagtat
    13921 ggtagcccct ctggcatgat atcgttgaag agaattttac caactgttgt aagcaagacc
    13981 ttatgtcttt gctcttctgt ccaaggcttg ttgaggctgt ctgttgcgat accaacacgt
    14041 gagtggaggt gaacataacc attgcggtaa gccataaccg cttcgtcacg gtctttgaag
    14101 accattcctt caccttcgcg accagcttct tccatggtca agtagtagtt acccaaaacc
    14161 atgtcctgag atggagtaac taccggtttc ccatctttcg gattcaagat gtgctcagca
    14221 gctagcatga ggatacgagc ttctgcttgt gcttcttctg aaagtggtac gtggatggcc
    14281 atttggtccc cgtcaaagtc agcattgtag gcttcacaga caagtgggtg caagcgaaga
    14341 gccttaccat caatcaagac tggctcgaag gcttggatac ccaaacggtg aagggtcggt
    14401 gcgcggttca aaagcactgg gtgttcttta atcacttctt caaggatatc ccagatacgc
    14461 tcatctccgc gttccaccaa gcgtttagct gctttgacgt tttgcacgat atcacgggca
    14521 acgatttcac gcatgacaaa tggtttaaag agttcaatcg ccatttcacg tggcacacca
    14581 cattggtaca tcttaagagt tggaccaacg gcgataacgg aacgtcctga gaagtcaaca
    14641 cgtttaccga gcaagttttg acggaagcgt ccttgtttac ctttaagcat gtggctcaat
    14701 gatttcaatg gacggctacc tggtcctgtg attggacgac cacgacgacc attgtcaatc
    14761 aaagcgtcaa ctgcttcttg aagcatacgc ttctcatttt gaacgatgat acctggtgca
    14821 tttaactcaa gcaaacgagc caaacggttg ttacggttga taacacggcg gtaaaggtca
    14881 ttcaagtcag atgaggcaaa acggccacca tccaactgca acattggacg aagatctggt
    14941 gggataaccg gaagaatgtt aagaatcatc cattcaggtt tgtttccaga cttgtaaaag
    15001 gcatccaaaa catccaaacg acggatggct ttgacacgtt tttgtccagt agctgttttc
    15061 aattcttctt tgagttcagc aatttctttt tcaagatcta cttgcttcaa aaggtcttgg
    15121 atggcttccg cacccatctt ggcaacaaat gaaccataac catattcacg caagcgctct
    15181 cggtattcgc gctctgtcat gatagacttg tgctcaagtg gtgtatcctt aggatcaatc
    15241 accacataag ccgcaaagta gataacttcc tcgagggcac gagggctcat atcaagggtc
    15301 aagcccatac ggcttggaat ccccttgaag taccagatgt gagatacagg agctttcaat
    15361 tcgatatgtc ccatacgctc acgacgaact ttcgtacgcg ttacttcaac cccacagcgg
    15421 tcacaaacaa ttcctctgta acgaatgcgt ttgtacttac cacaagcaca ttcccagtct
    15481 tttgtaggac caaagatcac ttcatcaaag agtccttcac gttctggttt caaggtacga
    15541 taattgattg tttcaggttt tttgacttct ccataagacc atgaacggac tttacttgga
    15601 gaagctaggg tgatttgcat acttttaaaa cgatttacat caaccactat ttcttccctt
    15661 tctattctaa gtgaactgct tattcttgtt cagcagcttc ttctgttgct tccgcttttg
    15721 ttgctttctc agcttcttca gcttcaaagg ctgctttagc ctcttgagct gctttttcgc
    15781 gggctttttc aaggtcatct acgtggatga catcttcgtc cattccttca tccaagtcgc
    15841 gaagttccac ttcttggtca tcttcgtcta ggacacgcat gtcaagacca agagattgca
    15901 attctttgac aagaactcgg aaggattctg gaacacctgg ttttggaatt ggtttgcctt
    15961 ttgtaatagc ttcataggct ttcaaacgtc cgttgatatc gtccgacttg taagtcaaga
    16021 tttcttgaag gacatttgac gcaccgtagg cttcaagagc ccaaacctcc atctcaccga
    16081 aacgttgtcc accaaactga gctttacctc cgagtggttg ttgggtaaca gttgagtaag
    16141 gtccgactga acgcgcatgc aatttatcgt caaccatgtg gtggagtttg atcatgtaca
    16201 tgactccaac agaaacacgg ttatcaaatg gttcacctgt acgtccatcg taaaggattg
    16261 tcttggcatc gctatccata cctgcttctt taacagttga ccaaagatct tcagaacttg
    16321 ctccatcaaa gactggtgtc gcaatgtgaa taccaagagt acgagctgcc ataccaaggt
    16381 gaagctccat aacctgaccg atattcatac gtgatggcac cccaagtggg ttcaacatga
    16441 tgtcgactgg agttccgtct ggaaggtaag gcatgtcttc tacaggaacg atacgagaga
    16501 caaccccttt gtttccgtga cgtccggcca ttttatctcc gaccttaatc ttacgttttt
    16561 gagcgatgta aacacgaacc aacatgttaa cacctgattg caactcatct ccatttacac
    16621 gtgtaaagat cttaacatca cgaacgacac catcggcacc gtgtggtaca cgaagagaag
    16681 tatcacgcac ttcacgagac ttgtctccaa agatagcgtg caagagacgt tcttcagctg
    16741 aaagatcttt ctcaccctta ggtgttactt tacctacaag aatatcacct tctttaacct
    16801 cagcaccaat acggataatc cccatttcgt caaggtcttt gagggcatct tcaccaacgt
    16861 ttggaatttc gcgagtgatt tcttcaggcc caagctttgt atcgcgcgtt tctgattcgt
    16921 attcttcaag gtgaacagat gtgtagacat cgtccttcac caagcgttcg ctcatgataa
    16981 cggcatcctc gaagttgtaa ccttcccaag tcatgtaggc aacgattggg ttttgtccaa
    17041 gcgccatttc tccattttcc atagaaggtc cgtcagcgat gaaatcgcct ttttcaacga
    17101 catcaccaac ttttacgaga gtgcgttggt tgtaagcagt acctgagttt gaacgacgga
    17161 atttttggat gtggtaaaca tccaatgaac catcttcacg acgaacttct accttgtcag
    17221 catctgcgta agtaacttta ccatcatact gagcaatcac agccgcacca gaatcgtggg
    17281 ctgcttggta ttccatacca gtaccaacgt aaggtgcctg aggattaatc aatggcacag
    17341 cctgacgttg catattggct cccatgaggg cacggttgga gtcatcgttt tccaagaaag
    17401 gaatacatgc tgtcgcaacg gcaactacct gttttggtga aacgtccatg tagtcaacaa
    17461 tattagctgg atactcttgg ttgacccctt ggtgacgtcc catgacaatc ttctcagcaa
    17521 aggttccatc ttcattcaga ctagagttag cctgagctac agtatattca tcttcttcat
    17581 cagctgtcaa ccaaacaatt tcgttcgtga caacacctgt ttcacggtca accttacggt
    17641 atggtgtttg aacaaaacca tatttgttca agtgtccgta agatgacaag ttattgatca
    17701 aaccgatgtt aggtccttca ggtgtctcga ttggacacat acgaccatag tgagtgtagt
    17761 gcacgtcacg tacttcatat ccagcacggt cacgagtcaa accaccaggt cctaaggctg
    17821 acaaacggcg tttgtgagac aactcagaaa gcgggttgtg ttggtccatg aactgtgaca
    17881 actgtgatga accaaagaat tctttaactg cagctgttac aggacggata ttgataattt
    17941 gttgtggtgt caagacttca ttgtcctgaa cagacatacg ttcacggaca ttacgttcca
    18001 tacgagaaag tcccaaacgt acttggttgg caagcaattc accaaccgca cggatacgac
    18061 gatttccaag gtggtcgata tcatctacac ggccaagtcc ttcagccaag ttgaggaagt
    18121 agctcatctc agcaaggata tctgcaggag tcaccgtacg aaccttgtca tctgggttag
    18181 cattaccaat gatcgttacg acgcgatctg gatcagttgg agcaacaacc ttgaattttt
    18241 gaagaacaac aggctcagtc acaacggctg catcgtttgg gatgtagaca atcttgttca
    18301 agtcgccatc caaatggctt tcaatgcttt caatcacgct acgagtcata atcgtaccag
    18361 cttctaccaa gatttctcca gtttcagggt ctaccaatgg ctctgcaatg gtttggttga
    18421 gcaaacgtgt tttaacattg agttttttat tgattttgta acgaccaact gctgccaagt
    18481 catagcgacg tgggtcaaag aagcgagcca caagcaagct acgtgagctt tcagctgtct
    18541 taggctcacc tggacgaagg cgttcgtaaa tttctttcaa ggcttcgtct gtacgagagt
    18601 ccattggatt cttgtggata tctttttcaa cagtgttgcg aaccaattcg ctgtcaccaa
    18661 agatatcaaa gatttcatca tcacctgaga aaccaagagc acgaaccaag gttgtaaatg
    18721 gaatcttacg agtacggtcg atacgagtgt aggtgatatc ttttgagtcg ctttcaagtt
    18781 ccaaccaagc tccacggtta gggataacag ttgaaccata gcccacctta ccatttttgt
    18841 ctactttgtc gttaaagtaa acacctggtg agcggaccaa ctgagaaacg ataatacgtt
    18901 caccaccatt gatgatgaaa gtacccattt ctgtcatgat tgggaaatca ccaaagaaaa
    18961 cttcttgggt cttgatttcg cctgtttctt tattgatcaa gcggaaggtt acaaaaattg
    19021 gtgctgagta gctagcatcg tggatacgag cttcttctag cgtgtatttt ggttccttga
    19081 tttcatatcc aacaaattcc aactccattg tgtctgtgaa gtttgaaatt ggcaatacat
    19141 cttcaaacac ttccttaaga ccgtggtcta ggaaagcttt gaatgagtca gtttgaattt
    19201 caatcaaatt tggtaagtca agaacttctt tgattcttga aaaactacga cgggtacgat
    19261 gtttcccgta ttgaacgtca tgtcctgcca agatgattct cctttgtaaa taagttccaa
    19321 gccttgtcaa tcaggctttt ctaatcgcca tatggttgta aaccccttat caccgtgtcc
    19381 tcttgacgaa ttttcagaat ctttaagcct ctgttacaaa tgctcaaaat cttgaaaaaa
    19441 agcacaaaaa gagcagctaa atctgacttt ttcagaagat ttaactgctg tgagccttgt
    19501 ctggacaata tttcagacaa aacctacgac aaatgattac ccatattata ccctatttag
    19561 ctagattttt caaggggatt cagtaggttt ttggtaattt ttttcccata gaaaacttgg
    19621 catcacattc gaatcacgct atggtacaaa aaactgaaaa aactattgac tgaaaatcat
    19681 tttcaaggta taataataaa cgttaaggcg gtatagccaa gtggtaaggc acggctctgc
    19741 aaaagcttga tcgtcggttc aaatccgtct accgccttct ataacttgat ttatcaggtt
    19801 tcaaatgaac agaaagccca atttgaaggg ctttttttat tttccctcga ataaatacgt
    19861 ataactttaa aaacttttgg agcgagtttg tggcagagtt ctttccatgg cataattccc
    19921 ttttgaaatc aattgaaaaa agcttcgtca cgcctggtga caaaagcctc tagtttattc
    19981 tgtttcttct tctatttcga cttctgtgat ttggactttt accttggtaa cacgtccatt
    20041 cttcacttta tcattggtta agataaactg tttgttttgg ctgaccaatt catagctgag
    20101 tttctcagtc gttggaatgg ttcccactcc tgtcaaataa taaccagcga tagtatcaac
    20161 gtcatcactt tccagttcaa cattaaagta attattgaaa tcattgaggg tcatagttcc
    20221 ttgaacaatg taggtatcct cgccaatctg atgaacatct attgctgctt tgtccgtttc
    20281 atcatcaatt tcaccaacaa tttcctctaa gaggtcttcc aaagtcacca aaccagccat
    20341 accaccgtat tcatccagca agattgccat ttgtctttgg gtatttcgca gttcttttag
    20401 caagtcatcc acaaaaatag tttcaggtac aaaaagtgga tcttgtaaaa ttctcttcca
    20461 aacaatattg tcaaaaccgt ccacaaagcc tgccttaagg agactcttgg tgtgaatgat
    20521 tccaattaca ttgtccttat ccccatcata aaccgggata cgagaataat tttgttttaa
    20581 aatactttgg ataatggctt gactatcatc ctgaatatcc accataaagg catccgttcg
    20641 aggaaccata acctctcgtg ccatcagttc atcgagcgaa aagacacctt gtaacatctc
    20701 aatctcatca gcatccaatg tttcttcact atttgtcagc atataggcaa tttcatcacg
    20761 ggtcatcttt tcatccgcat catcgaatga cataggagtc aaatggctca agaaattggt
    20821 cgaagcagct aaaagccaaa caaaaggact gactagtttt ccgatcccaa tgataaccgg
    20881 cgctgtacga attgccaagg catcctttag attaagagcg attctcttag gatataattc
    20941 cccaaaaacg atggaaatat aggtcaaaaa tgccaaggat agaaaagttg ccacggcttg
    21001 tgctgtttcg ccattcccaa gccaagaggc aatcacacgt cctagagtat cagttaaact
    21061 cgcccctgat aagattgtaa tcagggtgat tcctacctga atggttgata aaaagtggtt
    21121 aggattttct agtaccttca gcaggcggat gtagcgtctg tctccttctt ccgccttttg
    21181 ttcaactcgg gcacgattaa gagaaaccat ggccatttct gtggctgaga aaaaggcatt
    21241 taataccgtc aagataaaca ataaaacaaa ttgtagcaac aaattctgac tacttgggtc
    21301 ttccatagtt cttctcctaa aatagtatct tatcctttat tatatcacaa aatttaatcc
    21361 aatacatatt atttttttct tagcgtaact tccatctctc tcccaaaact ggaagttagt
    21421 ctaagacgga tttccactga tttcacgcgt ttttctcata ctaaaagcta ggttgtggga
    21481 aactggaagt tagttttaga agttactatt ttttcttagt acctgttcta gccctgctta
    21541 aactagtaaa aagaactcct acatccataa gtgacatagg agtttaggct tatcttttac
    21601 tgagttacag ttacttctcc agttcggtaa tccagttgaa gtcccttttg aacattggga
    21661 actagaacat tgaattccaa ttctccatcc gaagacttgg cttcaatctg tgaagctgag
    21721 ggaactaaat cctcgtttga agcgtagtaa agggttacac ggtaacggac acgcttgttt
    21781 tggtccaagg ctttacgcac cttgctttca tagtagtttt gaccagtcga atactcggct
    21841 tgtgcctgat ttgcccaggc tgtctgaaca gcaatgtttt taggattgct tgttgaggca
    21901 tcaaaaccat ccaaaccacc gattaaggca tagcctaaca aatgacctct atcgactgca
    21961 tgggtataag agccctttag attcttgacc tgatgccaac ctggaggagt ccaagaagtt
    22021 gaaccattcc cagtttcttt acgattcttg tactgacgag tggccttaga caagagggca
    22081 ttagctacgg ttggaacagt ttccttaccc actgtctttg ttttattgtc agcgtagggc
    22141 ttacttgaaa ccttggcatc tagatttgtt ttattaccat tgacgataaa agcacctgag
    22201 ccattccact ccagactccc ctttatttga ctcttgactg cgtctgttaa gacactctct
    22261 gccaatgctt gactaggagc ttcaggcgct tgttttttct gactaagctt ggttttagga
    22321 ctattagctg tcgactgcat ctgcttgata taatagctcc ctgtagacaa aagcaataac
    22381 actagcagtc cgattagtgt ctgtcttgtt tttttgttca tatttctcct tatctttaga
    22441 aaaaggctgg ttctccagcc taatttcctg taaatttatg aatcaattcc tgccattttg
    22501 ctggagacag gatagcccat gaatcttgac ccttgcccaa gattccataa cctaccatta
    22561 aaccgattcc tagagccaga gtacctaaaa tcagtactat gatgactaaa agtaaacgct
    22621 tgactacgta gcttgatttc ttattcatct tcatcacttg cctcaatccg ctgaatctta
    22681 gcacctagct gcgccaactt ctcatggaaa ccgtagtaac ctctatccaa gtgaaccaat
    22741 ttaccgacca cagtttctcc ctgtgctacc aaacctgtca aaatcaaagc tgcactggca
    22801 cgaaggtcag ttgaaagaac ttctgctccc tgcaaaggct gtccaccaac aatacgagct
    22861 gtatcacgga taatctcaga atgcaagccc atgcggcgca tctcttctag gtgttggaaa
    22921 cgattttcaa aaactgtctc caccatggtt gattcgcctt ttgcaactgt catcaaggct
    22981 gtaaattgag cctgcatatc tgttggaaat cctgggtggg gcaaggtttt cacatgaaca
    23041 gcttttagat tttccagttg agaacgaaca cgaattcctt cgtcttcttc aattacttca
    23101 acacccattt caagtaactt ggcaatcaag ggacggttgt gctcccagac agcgtctcga
    23161 atcaagacat caccaccagt catggcagca gctaccataa aggttcctgc ttcgatacgg
    23221 tcctggacta cattgtgagt cgtaccatga agtttctcaa caccagtaat ggttatagtc
    23281 tctgtaccag cacctttgac cttggctccc atttcattaa ggagaatggc taagtcaaca
    23341 atctcaggct cacgcgcagc attctcaatc actgtcaccc catcagccag agtcgctgcc
    23401 atcatcaagt tctgcgttgc accaacactt ggaaagtcca tatagatatg agcaccatgc
    23461 aagcgttctg cctttgcttc gatgtaacca gctgtctgac taatcttaac ccccatagct
    23521 tccagacctt tcaaatgaag atcaatagga cggctaccaa tcgtacaacc acctggcatg
    23581 gataccttgg catgacccac acgggcaagg attggcccta atacaacgat ggaggcgcgc
    23641 atcttactga catacttgta aggggcttcc tcagtgatgt cgccagtagc atccaccttg
    23701 acaagatgag cttcctcatc aaagtcaacc ttggcattca aaccaccaac tacctgattc
    23761 ataataaaga catccgacaa aatcggaaca ttctgcaaga cggtctttcc ttcacttgct
    23821 agaatagtcg ctgccaacaa gggtaagact gcattttttg ctccctcgat cgtcacgctt
    23881 cctaccagac gattatcgcc accttgaacc acaattttat ccatactcgt ttcctttact
    23941 cttgatttta taataatcct ctaagcgctt cttgccacaa atgatacagg ctaataaaga
    24001 aagaactcag gatgtagcct atgacaatgc taaaaaaggc tactaataca cgaacttttc
    24061 ttgtattctc agctgtcact tttaaaacct tttcccatct aacaagatcc tttaaaaggt
    24121 aaaaactcac ataaataaag agcatatgac tacttagagt gaataataat tgaaccattt
    24181 gactattata ccatcttctc tgtttgtgcg ctagtttgga aacttcactc aaaaattagg
    24241 gattgttttt caagtaatca atcgcatctt gtagggtttt aacgggcacg attttcatat
    24301 ccgtcttgat tgttttagcc gcttctaggg ctgtttggta gttgtttttc gcgtccggat
    24361 gcgccttttg ttcttcttcg ctaacagggt tatcaggagc aaagaaaata gcagcacctg
    24421 ccctagccga agctacaacc ttcttatcaa tacctccaat gtctcccaca ttaccatcgc
    24481 ggtcaatggt acctgtaccg gcaacaatac gaccattacg aagatctggg tgagctattt
    24541 gagtatagat agctagacta aacatgagac cagcacttgg gccgccaata ccagctgttg
    24601 aaaagctaat tgggacattg ctgattacct ctgtacgatc aatcaagccg attccaatcc
    24661 cattttttcc attttccaag gtgataatct tcccttctgc agacttggtt tgcccatcct
    24721 cttcataggt gaccttgacg gaatccccta atttttgaga actgacgtaa tcaatcaagt
    24781 ctttggaact atcaaaggtc tgatcattga ctgctgtgac tgtatcagag atattgagaa
    24841 tccctttaaa ggttgaatta tccgtcacat tcaaaacata aactccaaag tacttgagtt
    24901 cgatatcctt accagctgtt tttagtcctt gatacttggc catattttgc gatgtttgca
    24961 tgtagaattg attgattcgc ataaattcaa catcggaaga accacctgta gtctcctgag
    25021 cactacgaat atctgtaaaa ggtgtcaacc aagcataaat catatgagct aaagtagcat
    25081 gttgaacacc aaccgtaacg aattgatagg caccagcttc cttatcttct gtgtcattta
    25141 ctttaaggac ttggcgaata tcttccgaac cacctggaac ctctatataa taaggcaagg
    25201 gcactacaaa tgccaagaaa gtcacaatca aggccgcaat gacgtataag ggccatctaa
    25261 tctttttttt catttcttat ttcctccaaa atactctcgg gaacatagca ggcaatatcc
    25321 tgaccaaact tcaaaagctc tctaacgcct gatgaactga tatagagatg ttcaggtcga
    25381 ctatgtaaat aaatagtctc tatatcagaa gacagctgat gattgtagta atcaaaactg
    25441 gcttcatatt gcaaatccga cgcattcctc aaaccacgca ctagacaagt agcacccaat
    25501 ctttttgcaa catcgaccac caattcatca tgagaagcca cgacttcaac attttccaaa
    25561 tgtcccaaag ccttttctag cccccgttta cgattttcaa gagggagaaa tccttgtttg
    25621 tggggattaa aaaaaatacc cacacaaagc ttatcaaaaa gtctgctcgc ccgttcaatg
    25681 atatccagat gcccatttgt catcggatca aatgagcctg tgaataagcc aatcttatct
    25741 gacatagact gtcaccttac taattccata aatcttttcc ttccagatac ccagacaggc
    25801 aatttcttct ggaagttcaa cggtcttatc cgtctcgcac acaaccataa catcttcaga
    25861 aaaaagctct ctctcagcca ttttttcaat atctgctacg atttgttcct tggcataggg
    25921 agggtctaag aaaacgaggt caaattcccc agatacctgt tccaatgccc tttctgcatc
    25981 cattttgagg agttgaaatt ttccaacttc cttggtcatc tggatatttt cagccacgat
    26041 ggtctgagcc ttacggtctc gctccaccaa aacagcactg gacatgccac gcgatactgc
    26101 ttcgatagat aaaccaccac tacctgcata aaggtccaag actcgtccca cttcaaagta
    26161 gggaccaatc atgttaaaaa tggctcccct aaccttatcc gaagtaggtc ttgttgtctt
    26221 gccttctagt gtcttgaggg gacgtccccc atagattcct gatacgattt tcatactgtt
    26281 tattatacca aattatagac aaaaagagaa agaaaaccga accttgcggt tcgattctct
    26341 acaaaatatt ttcgtaagta tcgcggactt cttgaggcca aacacttgtt tgcacttctc
    26401 cgatgtgtct cttgcgaagt aggaacatgg ccatacgaga ttgtccaatt cctccaccga
    26461 ttgtcaatgg gaataggcca ttcaacaaag acttgtgcca ttccaattct aagcggtctt
    26521 catcacctgt aatttccacc tgacgtctaa gagtttcttc atctacacga attcccatag
    26581 aagacaactc aaaggctcca cctaaagact cattccagac aagaatatca ccatttagac
    26641 ccttgtagcc attctcagac tcgcttgtcc agtcatcata gtctggtgca cgtccatcgt
    26701 gcggtttacc atctggcaac tcgccaccga taccaatcaa aaagacggct ccaaattctt
    26761 tacaaatcgc attttcacgt tctttaggtg tcaagtctgg gtagcgttct accaattctt
    26821 ctgtatggat aaaggtgatt tgttttggca agatagactc gatgtcatag cgggcttcaa
    26881 cagctagctc agtcaggcga atagccttat aaatcttctc aactgtttct tttagataaa
    26941 cgatgttacg cttaccattt gggataacct tctcccagtc ccactggtca acataaacag
    27001 agtgggttgc atccaaggaa tcctcatctg gacgaagggc tttcatgtgg acaaagagac
    27061 cctctccttc accaaagcca aaacgagcca aggtgtggcg tttccattta gcaagtgagt
    27121 gcaccacttc ataagtagca tcagggattt ggagaacctt gaccgatact ggattttcca
    27181 caccagacag gttgtcctgc attccgtcac cgaccttact caagatagga ccttgaactt
    27241 cgacaacttc tagcttatct ttcaaatact gggtaaaagt gtttttgaca aaggaaattt
    27301 cttcttgttg atggataaaa cttttcttca tagacaactc ctcaaaaatt taataaacgt
    27361 attatacacc tatttccaag aaaagaaaag agaaaatttt aaaaaaatca gtgtcactca
    27421 aaacactgat ttcatagacc ttttagtcat tgcgaccaaa gatacgtaga atacttagga
    27481 aaagattgat aaaatcaaga tagatgctga gagccattga caccacccag cctgtcgcta
    27541 cacgaccttg tgattgttca taggcgaggc gaatcttttg gttatcccaa gcaatcaacc
    27601 ctgaaaaaac caaaaccatg gctacgctaa tcatataatc aaagaagcca ctagccaaga
    27661 aaatattgac taccatagca atcagtaaac caatgagagc tgccatcatt gctcgaccaa
    27721 tcccactcaa gtctttcttg gtaaaaatac caactgccgc catgacaaag aagagaaggg
    27781 cgctcgatac aaaggcagat aaaactgtac ccggagtata gaaagccacc acaaaactaa
    27841 gggtaaagcc gtttaaaacg gagtaaagta aaaatactgg aagagcagct ggactatttc
    27901 ttgaagccat gctactggca acgaaaacca aagccaactc cgcaaaagta gcaatggtca
    27961 accagagacg cccctgcatc aacaagtaaa ccaactgaga ctgaaagacc gtcaacataa
    28021 ggcctgatac caaagcagat agtccgattc ccagaccaac aaaggcataa accttagcgt
    28081 aaaattgatt gagacctgca cggtcatgta taatagtgtg attcatcctt ttctcctttt
    28141 ctgtgaacat ctttccttga ttataacaaa aaaagttaaa attagcttaa atggaagatt
    28201 aaaataccgg ctgcaactgc aacattcaaa ctctcggcct gccccttcat gctaatatgg
    28261 accaactggt ctgcactttc agccataagg ggactaattc cttgaccctc atttcccata
    28321 actagtacaa aattttctat agaaggcagt tctctgtaat caacagaatc tttagatagg
    28381 gttgttgcta gcactgggat agctaccttt ttagtttcgt caagaagcgc ttgactagtc
    28441 atccggtaaa tgggcagatg gaaatgacta ccttgcatgg aacgtagggt tttgagactg
    28501 tagatgtctg ccgacttatc tgaaacaatc actcctgtaa aacctgctgc atccgcagtt
    28561 cgaatgatag ttcctacatt accaggatct tgcacatctt ccaaaaacaa gaacttgccc
    28621 tgactcaaat cagcttgtcc tacttcttct ttttgaacca cggcaacaat tccctgtgga
    28681 gtctgagaat ccgccaaatc tagcaaaata tcctctgaaa tccagacagt ttgcggaaaa
    28741 gcagctaact gatttcgata attttctagg gcaaagattt tctcaatcgt cactccagct
    28801 tggacagctt cttcaaacaa gtgccagcct tcaatcaaat aggcagactt gcggtatttt
    28861 ttttggtgta atttcttggc attttttacc acagaattgg cttttgaggt tataatagtc
    28921 atagaaatat tataacacaa tcaaaggggt ttggtatgca aaaggttaga atgattgccc
    28981 aaggtagggt gcagggagtc ggctttcgtt ggggtgttta tagcctggcc cttgaaatcg
    29041 gtggcatcac aggtcgggta tggaataacg acgatggcac agtggaaatc ttagcccaag
    29101 cagactcatc tgctatcatg gcaaaattta tccaagaaat ccgaaaagga ccgacacctt
    29161 tttcaaaagt aagctactta gatgtcaaac taagcaactt tcctccctac tctgacttta
    29221 aaatcgcaaa ttaggtctcc agaactattg tatattttgt aaaaaaacag tagaatagaa
    29281 aggtataatt tttaaagaag gaataaaaac agtgaaatct attaaacgtt ttgcattctc
    29341 ggctatggga gtggctatgt tgcttgtctt gactggctgt gtcaatgtcg ataaaaccac
    29401 aggtcagcca acaggattta tttggaatac gatcggagcg cctatggctg aagccatcaa
    29461 gtacttcgct actgataaag gtctaggctt tggtgtcgct atcattatcg taaccattat
    29521 cgtacgcttg attatcttac cacttggtat ctaccaatca tggaaggcaa cgcttcactc
    29581 tgaaaagatg aacgctctca agcatgtcct tgagccacac caaacacgtc tcaaagaagc
    29641 aactactcaa gaagaaaaac tcgaagccca acaagccctc tttgccgctc aaaaagagca
    29701 cggcatcagc atgtttggtg gtgtaggatg tttccctatc ctccttcaaa tgcctttctt
    29761 ctctgctatc tactttgctg cccaacatac tgaaggggtt gctcaagcaa gctacctagg
    29821 cattcctcta ggttctccaa gtatgatttt ggttgcctgt gctggtgtcc tttactatct
    29881 tcaatcgctc ctttcacttc acggagtaga agatgaaatg caaagagaac aaatcaagaa
    29941 aatgatttac atgagcccac tcatgatcgt cgtcttctcc ctcttctcac cagctagtgt
    30001 cacactttac tgggttgtcg gtggtttcat gatgattctc caacagttta tcgtcaacta
    30061 tatcgttcgt ccaaaacttc gcaaaaaagt ccgtgaagaa ctagccaaga acccaccaaa
    30121 agcaagtgct ttctctaaac caagtggacg aaaagacgtt acccctgaac aaccaactgc
    30181 tatcacaagc aagaaaaaac acaaaaatcg caacgctgga aaacaacgtt cgagataaga
    30241 agaaaaaggc ttagctgatt tgctaagtct ttttgcaaat caaaagcccg atgtttccat
    30301 caggcttctt ttctatccat gtacacgttt catataatct tgataacttt cggtatccat
    30361 gagtttttta gcgttcttga cgcgatctgc tgttggtggt ttgactcctt cgagggaata
    30421 tggaattcca agttcacgcc acttgaactc acccatggtg tgataaggta gaatttcaaa
    30481 cttatcaaca tttttgaggg tcttgacgaa cttaccaagt tcaatcaagt catcatctct
    30541 gtctgtcaat cctggaacta gcacgtggcg aatccagaca ggttttccaa tatctgatag
    30601 atactgggca caagccaaga tatttttatt agtttggcta gtgacaatct tgtgctgttc
    30661 ttcgttgatt tccttgatat ccaaaagaac caagtcagtg acagccatga gtttgtcaaa
    30721 cttctcaagg taacgtggtt tattacggaa aggaagagca caggtatcca aggtacagtg
    30781 gattccttgt tccttagcct tggtgaagag agcaatcagg aaatcaatct gcaagagagc
    30841 ttctcctcca ctgactgtaa tcccaccctt atttccccag aaaccacggt agcgcaaggc
    30901 ctctgtcaag acatcatcta ccgtccgttc acgtgactta ttggactcca tagcccaagt
    30961 gtctgggttg tggcaatact ggcaacgcat gtgacagccc tgcaaaaaga caataaagcg
    31021 aataccaggc ccatctactg acccaaagct ttctgtcgaa tgcaccattc ctgtcacttg
    31081 tccataatca attgtttctt cagacatatc gttaccttcc ttgaaaacgt tttatagttt
    31141 tattatatca cgacttgaag gaaaaacaag atttttgcct attttttaga aagcaatctg
    31201 tttttcaatt tcattttcaa tctattatca gattattctt caaaatcgaa gccatataag
    31261 gttgcaaaat agtcctcagg gcgctctgca cggcggattt gacgggcttt accttcttcg
    31321 gtatagagga tttccgcaga acgtaatttg gcattatact ggtagcccat tgaaaatccg
    31381 tgggcacctg tatcatgaat taccagcaaa tcaccgattt ctgtatgagg cagttcgcga
    31441 ttgactgcaa atttatcatt gttttcacag agtgaaccaa ccacatctac cgcttcagct
    31501 ggtccatctg gatgggtcac gttgctaata tgatggtaag ctccgtacat agctggacgc
    31561 atgaggttga ctgctgaggc atccacacct agataggtac ggtaggtttc cttcttatga
    31621 gtcactcttg tgactagagc accgtgaggt gccagcataa aacgacccaa ttcggtgaaa
    31681 atcttgacct gaccaagacc tgctgacgta agaacttctt catacacctt acgaactccc
    31741 tcaccaatca aggcgatatc gttcggctcc tggtctggac gataattaac accaataccg
    31801 ccagaaagat tgataaagtc tagcgaaatg cccaactttt ccttgatttc aacagccagt
    31861 tcaaagagct gacgagccaa ctctggataa tagagatggg tcacggtatt ggacgctagg
    31921 aaggagtgaa tcccaaaagt cttggctcct ttttccttca agatagcaaa ggcttcaaag
    31981 agctggtcct tggtcatgcc aaacttagcc tccccaggat tgtccataat gtctgtcccc
    32041 agttcaaaaa cgcctccagg attataacga caagagatga tttctggaat gcctgctact
    32101 ctctccagat gttcaatatc ttcaaaggca tccaagttaa tggtcgcacc caattcacgt
    32161 gcataggcgt attccttgtc tggcgtgttg ttggaagaga acataatctc agaacccaga
    32221 aagtccagtt tatggctcat caaaagctct acataactag agcagtccac accacaacct
    32281 tcttcttgga gaattttcaa aatagctgga gttggagtag ccttaactgc aaaatattcc
    32341 ttaaagccct tgttccacga aaaagcttgg ttgacggctc ttgccttctc acgaatcccc
    32401 ttctcatcat acaagtgaaa gggagtcggg aactcggcaa caatcgcttc taactcttct
    32461 ctattgataa atggtgtttt cataagcttc cttctattct gtttgcaaag aaaggctggg
    32521 acaatcgttc caacctttag ttctatctct tatttatctt cgtcaaagat attgccaacc
    32581 tcaacatcga tggcttggtt cttgacatca atattggtta ccttcaagag tgacttgtag
    32641 tcaaactgct tttcacgttc ttcttgggca ttgtccgcaa agaccgctac acctgccagt
    32701 tctgagtcga actcgtgcaa gagactaatc ataccattga ccgttccgcc acctttcaag
    32761 aagtcatcca caatcaagac acggctgcct gccttaagac tacgttttga aaggaacatt
    32821 ttctcgatac ggtcaccact tgaacctgaa acatagttga cgctaacagt tgaaccttcg
    32881 gtaattttca ggtcacggcg cacaatgaca aaagagacat tgaggacatt ggcaactgca
    32941 tttgcaagtg gcacaccctt agttgctacg gtcataaccg cgtcaatttt ttggtccata
    33001 aagcttttgg caataatacg accaatattt ttcaagatgg ctggtgtgct aagcaaatca
    33061 gacagataga tataaccacc tggcaagata cggtcacttt ctgacaactt ggtacgcaag
    33121 tcttcaacca tttccttagc atcctgactc gaaatagacg gtgtgaagat gacacctccg
    33181 ccagccccag tcactgtctg gatatgaccg atttcaattt cctcaaaggc gcgtttgata
    33241 atgacgatat cttctgagat ggatgattta gcagactcat acttttcagc aaaagtattg
    33301 agactagtta gtttataagg attattaatc aaatagttgg aaatgacaac catccgatca
    33361 cttcttctta atttcatttc tatttcctat tccattttta ttcaaaaaat caaaaagcaa
    33421 actagaaagc tggtcgctgg tggttcaaaa cactgttttg agattgtcaa tagaactgac
    33481 aaaccctgta atatacctgc atatatacat acgacaaggc gatactaccc tagtttgaag
    33541 agattttcga agagtattca tttttgtctt ttacttatta taccatattc acataaaaaa
    33601 acgaacattc ttatcctaaa aaatgctcat ttttcttaaa ttatcaatct aaatctggtt
    33661 tatagaagga acgattatcc atagcgaaga ttttattggt catctctcct ttatccacca
    33721 aagccagagc tgttgacatc atcatcatgc ttgcatccag attgtcaatc atatggataa
    33781 tctctgcctc cataatacgt ggacggactg gacttccata ttcaagcaag ccgtggtgac
    33841 tgaggatgac atgacgaagc aaaacgactt cttccttggt atcatcgatg ccgagttcca
    33901 taactgtctt ggtaatttcg ctatcaatga gagcgatatg tccaagaaga ttacctcgca
    33961 ctgtgtactc tgtctggtct ggccccgtca actcgataac cttagctaag tcatgcagca
    34021 taatccccgc atagagcagg ctcttattga gctgaggata aacttcgcta atagcgtctg
    34081 ccaaacgtac catggtcgcc gtatgataag ccaaccccgt ttcaaaggca tggtggttgg
    34141 tcttggcagc tggataggag tagaattcct tatcatactt ggtgtagaga tttcggacaa
    34201 tccgttgcca gacaggattt tcaattttga aaatcatttg cgacatgtag tcacgaattt
    34261 ccttgacatc aactggtgac ttgaccttga aatcggctgg gtcattgggt tcaccagctt
    34321 gaggcaggcg gagagtaatt tgattgactt gaggggtatt gttataaact tctcggcgtc
    34381 ctttcatgtg gacaacctta cctgcggtaa aggcctcaat gttatgaggt tgggcatccc
    34441 agagcttccc atcaatctcg ccactatcat cttggaaggt aaaggctagg tagtttttcc
    34501 cagctcgagt ttgcctcagg tcagctgatt tgattaggta aaagccttca aatagctcat
    34561 cttttttcat gtgactaatc ttcatattct tcctcatttt cttgaaaatg gagtagatca
    34621 agcgcaggct caccttctga caactcaatg tgacggagcg tccgctcgat agctatggta
    34681 cgacggttta ataattcatc aatattgcca gaggcatgtt ggagatgttt ttgtgccttg
    34741 accagaatac caccaaactt gccaaactcg gtcttgacac tggcaagagt cttgctgata
    34801 tggtcggcac tcttttggat attaagggtc ttgaaaccaa ctgatagaga gttaagaagg
    34861 gctgataggg tacttggtcc tgcaacaata atctgttctt cccgtctcaa atcatcaaag
    34921 aagaccggat tgcgcacaat ctctgaataa agaccttctg tcggaacaaa caagattcca
    34981 aagttggttg tccgaggtgg tgctatgtac ttgttcctaa tatccctagc aaagcgcttg
    35041 acgcttgcta ggagtgactt acgacagcgt tcaatctcat ccttgtcacc tgtctcatag
    35101 gcttcttcca agcggtaata atctgccagt ggaaacttag agtcaattgg cagatagacg
    35161 tattcttggt cgccttgtcc gggtaacttg atggcatact ccactcgttc actagagttt
    35221 tcaaccgttg cgtattctcg ttcgtactgg gcaggtgtca tgatgtcttc aataatttgc
    35281 cccagttgca attctcccag aatccctcgc gtcttggttc cagagagaac cttgttaaga
    35341 gctccgacat cacgggcaac tgtctgcatt tctccaaggc cacgattgac agactccagt
    35401 tgtttagaaa ctgtctcaaa ggaagcctgt aagcgtgtct gcaaggtctt ttctagtttt
    35461 tcctcgaccg tctggcgcat ttgttccaaa cgttgctcat ttgattcctg caaggcttgg
    35521 agacgttggt ctgtcttgtc tctagtttgg aggagattat ctgtcatttc ttgacggact
    35581 tgggtcagac cttggtgcaa ttcaatccgc acttcttgca aacggtcgct gacaaccact
    35641 tccaaatctt tttggtctaa ctggctggct tgtctggctt ggtcaaagcg gtaatccaac
    35701 tggtctgaca aatgatctgc ctgatcctcc aagctcttac ttaagtgttt ctcctgccta
    35761 tcctgccttt gccaaatcag aaagagacca gctagattgg caattaataa tagaataagt
    35821 aaactttcca tcctacctcc tgtccttgct atgcagtacg accacataac catctgggca
    35881 agttaccgac acttccctat ctatatattc gttagaagcg tacacttttt taaagaaaaa
    35941 attttcctct gtcaactcat acttggctcc aagaatagtc agctggctat cccgaactgg
    36001 cataaaggct agatagtcgt agtctgaacg aggttctagc tgactgattc cttctggaca
    36061 ataagtaatc aagttttgcc catcctcaat ttctatttga tgcatatagg atgccaactt
    36121 aggattgcta ggcagaaaga cattggccaa catatggtca atacggccac ccaaggcacc
    36181 gaaaatagta acctgagcct gaggattttg cttaaagatg gtcaagagag ccaattccaa
    36241 atcagtatca tctttttctg gccgggcttg gacaaaatac tgggcacgtt tttgaatcac
    36301 ctgtcgctct tcttccgtca cagaatcaaa atctccgact gctagagcaa gaggcaaatc
    36361 ttcttccaag atccagagcg agcctcgatc cacaccaaca aaaccatcaa agtccgtccg
    36421 ataatgaccg cggtctccgc ctgcaaaaac ggcaacccta gtccagttgt tttctgagag
    36481 tttgtactcg ctcattgaca tctcccttaa agacatagga acctgctaca aaaacggtcg
    36541 caccagcttc tttggcttga gcaatagttt ggtcatcaat cccaccatcc acttcaatct
    36601 caaagttcaa acctttttca tcacgaaggg caaccagctc acgaacctta tccatggttt
    36661 ctggcagaaa ggcttgacca ccaaatccag ggttaactgt catgactagg acttggtcaa
    36721 ctagatgaag cacatgctta atcgcttcaa ctggtgtacc aggattgata acaactgatg
    36781 gcttgacacc gagtgaacga atcttttgga gggcaccatg aatatgaggc gttgcttcca
    36841 catggatact aataatatcc gcacctgcac gcacaaagtc ctctaaatga tgctcagggt
    36901 ttgacaccat caagtggcag tcgaaaacca tcttactatg aggacgaaga gcttcgacca
    36961 cacccgcacc aaaactgatt tgcggcacaa aatgaccatc cataatatcg atatgggcat
    37021 actctgcccc agttgcttct aggcgtttaa tttcacgttc aaagttggca taatctgctg
    37081 ccagaattga cggagcaatc ttgtattgag acataggttt ctccttattt tggaattttt
    37141 ttgctgactt ttttataggt ttctctgcga ttttcaattt cgctgaggaa ttgcaggtag
    37201 ttgtcaaaac ggaagggcgc aatggtacct tcttcaactg ctggcttgac agcacaggac
    37261 ggctcatggg tatgggtaca agtacggaat ttgcagtctc gactgacagt ggcaatctct
    37321 ggaaaggcct gattgaggtc ttcagccgtt gatacttcat aatccagtga tgaaaagcct
    37381 ggtgtgtctg cgattttacc cccattgagg ttgtaaaaac taacagctcg agtggtatga
    37441 cgcccgcgac ccagactgtc tgaaatttct cccgtttcaa gattaagatc tggtgcgatt
    37501 ttattgagaa gagttgattt cccaacaccc gtctgcccca taaagaccgt aaccttgcct
    37561 gttaacaaag gcaggagttc ctctttactg gtcacaaagt catatccgat gtcaccatag
    37621 gtctgctggt aaaaatccag ttctcccccg tcttccaaca aatccatttt agaaatatag
    37681 acgatgggat gaataccctt atgctccaaa agaaccaaga aacgatccag caaattgctg
    37741 ttaaaatcag gttccttgac agacatgatt actacagctt ggtcgatatt gacaataggc
    37801 ggacggacca gactgttttt ccgttcgtga atcttgagaa tatagccttc tgaattttcc
    37861 tcggcagaga aatctaccca gtccccaacg taaggggtat gccctttttt acggaaattc
    37921 ccacgcgcgc gtgtttgata gacttggcca tcactctcta catagtagaa cccagccaag
    37981 gctttaatga tttgtccctg caaaataact cctttaaaat tgttagttcc attataacaa
    38041 aaaaaagcta ttttagctta gcttctcttt acaatttcga taagtctatt ctaaaagaaa
    38101 aagatacact cacgctttag ataccaagtt ttatacaaaa ataacgaatc tacctcctta
    38161 tataaaaaca ggcgaacaga gagcctaaaa ctccctgttc gctttacttt ttctgtgtgt
    38221 aatagaaaaa acctaataaa gcaagacaaa aaatatgaat ataaactcca tacatcatgt
    38281 ggggaaagac agactctata aaatagcctg gtagatagat gattggaaat tgatagaaat
    38341 cataggtcgt tagagatagt aaacaaagaa catacataaa tactgctaca attagaaccc
    38401 atccttgctt tcgccataga aaaatcatca ataagaacag acaaagtaga tagattgacc
    38461 aatttcgcaa cataaattga aaaccataaa tcatactttc agtcgtttct ccatcaatca
    38521 catcaactac tttatagaaa ggtaaacaat agctcactaa aaaaagaagg ggactaatta
    38581 tttttttcat attctctacc atggattatt ttgcaagtaa gctgaccatc tatctccata
    38641 tcgaatcaaa ttaacagtat aattagttac tacccgattt tcacctggtc caaccttaga
    38701 aaaacctgtg ctatactccc aactacttct tgcacctgga tgaaaccatt tttgctggga
    38761 tgaataccaa ccaccttgtc taacttccac acttgaagat tctatttcga tatccctttc
    38821 atggaagtca actcctccac ttattttagt aatatttttt tgtacacctc catcataact
    38881 cacccagtga acaacagtat acaattccac actataagta ctatcaaact gactttgatg
    38941 agtagtaatg ttttgagtat aagcgtgcac tttctgtata ccaaaaatat ggtctaatat
    39001 attcgttggt ttagcaagct tattgtaacc ctcttcaaca aaagtagcct tctctaaatc
    39061 agcggtataa acagtttttc cttgtactac ttgattgttt atagaagtaa tacggctatc
    39121 cacaacaatg ggggccacca tatctccatc gctagtttct attttagctg cctctttcac
    39181 ctcatataca actcgatttt cttcagactg aatcacttct accccatttt tttcaggtat
    39241 agataatcta gtttgcccat tagaaaccgt tatatttgga ccaatattct tctcatttgc
    39301 ataagtcgca ataggaaatc ctgatagaac cactgcacac ataaggctat tgactaattt
    39361 ttttctcatg aatatatcct tcccattttc aagaggagta aaaatagcta atataagatg
    39421 aacgtttcct tatatttcag aataggatat ctatctgaaa gcaattcaca ctcctataga
    39481 taactgttcc aaatcttggc tacatatatt tcaatagttt gctacaaaaa actcctttct
    39541 aagtctattg aacatgccct tattcaccta ctaatatatc acaaaaataa ataaatacaa
    39601 gactgttttt tctactagtt ttatgataga atagtattgt tagggtaaat ttcatatata
    39661 aatgtcgagt gaaaaatcga tggagcagaa atcggtagac aaagactcga tttcgttggt
    39721 ccagccccac aaggctaatc aggtatttca cgagtttaaa aatctaatga agtgcccaat
    39781 caactactga ttcattgatt taatacgaac tatagtggat tgaacctaga aatagtacac
    39841 taaggattct aaaacgtttc tagaaattaa tttgactttc ctaatctctt tgttcatatc
    39901 ttatttcaat ctactatata taccaaaatg ctaggacaaa agtctttaaa atcagagaaa
    39961 cgcatattat caggtgttgg aaaaccttga taatatgcgt tttatcatag aaagatctac
    40021 ttcatttttt actaaaatta agtttttgtc cctgcatctt agagtccttg ccctttaagt
    40081 gcatctgcta ggctggcaaa ctcttccaag ctgagagctt ccccacgcac acttggtgac
    40141 aagcctgctt ggtccaaagc cttggtcagc ttgtccttga cctcttcagt cttgccaaag
    40201 taacctgtca agttattcca caaggtcttg cggcgatggg taaaactagc cttggaaacc
    40261 ttaaagaaaa agttctcgtc ttccactgct acagctggca ctggacggcg caccattttc
    40321 aagatggctg agtctacatt tggtgctggc acaaagactg tacgcggcac gataaaggca
    40381 accttggctg tcatgtaata ctgcacagcg atagacaagc taccgtaagc cttggtattt
    40441 ggttgagccg agatacggtc cgccacttct ttttgcatca tgaccacaaa ctcactaaaa
    40501 ggaatgccac tctcgattaa gtgcatgaga ataggcgttg tgatgtagta aggcaaatta
    40561 gccactacct tgattggcag gtcaggattt ttgaaattct ggatatgttg cgccaaatcg
    40621 accttgagaa tgtcctcgtt gactacagtc acattgtcaa aatcacgcaa ggtatctgcc
    40681 aaaatcggta ccaaacgatg gtcgatctca aaggccataa cttctgctgc acgctcagcc
    40741 aaaaactcgg tcaaggcacc aatacctggc ccgatttcaa tgacattgac ctggtcatca
    40801 atctcagccg tatccacgat tttttgtagg atattggtat ccgtcaagaa attttgccca
    40861 aaggactttt taaaggtaaa accgtgacgc tccagcactg ccttggtcac gctataatct
    40921 gcaattctca tctattctct tttctattat ctgttactac tattgtaaca cattcccctt
    40981 gtattttggg ggtgaaaatc ataccaaaaa actatctttc ctcatgaaga aagatagtct
    41041 tggaaatctt taaaagtggc cttagcgctt ctctcgaaac atacgttcat actcttcgaa
    41101 aatctcttca aaccacgtca gcttcacctt gccgtactca agtacagcct gcggctagct
    41161 tcctagtttg ctctttgatt ttcattgagt atcaaaaaaa tcagcttctt gataacttcg
    41221 attttcgggt agggtcaaat ccacatcctc ctccacatca tccactgtca gttcaaccgt
    41281 ttgaggtaaa tttttcaaac gataatgctt ttgcctttgc ccttcactac tagcaaaatc
    41341 aagcaaatag ccatgcactc ggatttgaac caaaaggaga ggattggtca tattttccat
    41401 gatgaggata tcttataaac cacctctggc actaggataa tcagataccc tgcgaatacg
    41461 gattttttca cacatgagca agtttcctca tatcaatgat ttcatcagcc ttattccaga
    41521 ctaggggatt atgggtcgca atgatgataa ttcgattttc atctttcaaa tccaccaaga
    41581 gattcataac ctcctctgaa ttttcaggat caagagctgc tgttggttca tctgccaaaa
    41641 tcaagggtgg atttttcaaa atagttttcg caagggcaac tcgttgggcc tctcccccag
    41701 ataaagtata aattttttgt tctaaatcca aataccctag attaactttt tctaaagccc
    41761 ccacttgcct ttccaaacgt tctacttttg agattttctg accaacaaaa cccaaatcca
    41821 aattttcttt gattgattgg ttttctaaga ggccaaaatt ttgaaagaga tagcccatct
    41881 gatctcgaaa atactcacga gtgggaatgg tttttaaatc tttcccctga tagagaaccc
    41941 ttcctccatc tatcttttct agctttccca agatattcag cagcgtcgtc tttccgcttc
    42001 cactctttcc gattaaggca taaaccttgc ccttctccag cttgagattc aaaccagaga
    42061 aaatcgcgcg gtcattaaat ttcttttcca atccttgaat atcaatcatc ttattttcct
    42121 ttcaatactg ccatgctacc agcttcttct tttttgtcct gccttactaa aatcaagagg
    42181 gcgttaagcg taaacaaagc tacaactaga gcgctaatca aaccatctct tgtcaaaata
    42241 ctagatagga ctaggccaag caagagaact cctccttgcg ccagtaaata cttgccatga
    42301 agctcataga ttgtcatacc agcaagacgt ttaatcataa gctcccgtct gaactgctca
    42361 aaatagagaa gattcatgga atcaaataac aagatagccg tagacagggt caaaatcgtc
    42421 ccaataagga gagagtaaaa ctctacccgt ttgtcattta gtaccttggc aaaaaatagc
    42481 tggcttttta ccaagtaaga aaccttgtga tacagacctt gctcttttag agctgtgatg
    42541 ctgtctccat atcgatctag tttcaaggca ctattagccg tagttcccca caacatccct
    42601 gcaacaggtc ttgttccagt cgcttgcggc gttagaacta caataatcgg atcttttagg
    42661 tactggcgtg ttgtcttgta cccatcatta tagaggaaac gttcctgtcc tgtttctgta
    42721 aaagctagac ttacctgtgg gaggtagtat ttctgactcg tcacttctac actttcagat
    42781 gaaaagtttt gcaggtaatc tgtaaacaat ctttggtagt aggtagactg ctctcgcaag
    42841 ctctcaggca agattagccc aaactctccc tcagacaagt tttgcatctt gtccataaac
    42901 tctgaagaaa cggtaatctt ttcttctatc agatagcgtg gtgagacata gataacattc
    42961 cctgacggtg tgtagtcact gagacgattg ccatctaggt ccacttctgc tccgtctgag
    43021 aaattgtcaa cattgctcat aatataaaaa gagtctgtat tggctaaccg ttcttcagta
    43081 aatgtctgcc actcacgatt atccttacgc gttccttctt catcggcaaa tgcactagac
    43141 caaccaaagg atagacggta acggtctgag gactggctcc atttattgct agctctctcc
    43201 atttcctgca tttcacggta gtggggtagg agagctgtcg cactcgatcc gaccaccaat
    43261 acagctaaga gttgccccac catcatcaat gtcatcatac gtttgagagg gagtttccct
    43321 ttcaatagat ccaccagact attttcctgt aaaccaagta gatagacgac acttagtaag
    43381 gtagaaatcc ctgccagggt caatccataa agtagaagag caatgatgac cagttgcacc
    43441 gttgccataa acaaggcacc ttgataccag agaatcccca atcccaaaag actggatacc
    43501 agcactgagc agataaactg tctcacatct tctaacactg gtctgagagc aactccaaac
    43561 aagctctcac cagctattaa gcgaatccct gcctgacgaa gggatttgat ccgataaatc
    43621 agggtcagac tcataaaggt cagcagaaaa atagctaaac tcagtagcac cataggggtg
    43681 gccgccaata gtaagactat cgaaaatgga tcttctccat tcgaaacaaa gccttgatag
    43741 ccaagttctt tcaaggtggt ctgtaagctc actccatcca aacttcctga cacaatcaag
    43801 taagacccta ataaatcact cgtttctgca ctctccttgg aggaaatggt cagaccttct
    43861 ggaagctctc cctcaccata agtggcatag gtaaagagtg tttctccagc ttcatttggc
    43921 tcaacgattc gacgggcgat gagactatta tgctctaccg ccaagtgact caaaacctct
    43981 gtcacctctt cgcgactagc tactggttga tttatcatca ttcctgtcac atcaacagaa
    44041 ggatagttac caaataaaat ctcttctcga tggtcaacgg aagttatcac catataaagc
    44101 gataccaata ccattgaaat caaaataaat aaacgtttca tcatttcctc caaaaagtta
    44161 cttggtcact tagtgagaag gtgaccacag tcactgcgat gttaagaagc ttattaagtt
    44221 ctttctacta gcaacatctt caaatacatt atatccgttt tttcaagaac tatcaaggac
    44281 atcaatgtcc ttaaaatcta actatttcat attattttta gcttatatct aaaaaaacag
    44341 ttctaaagac gaactttaga actgttttga tacgaggaca aggacatttt tgtcactgat
    44401 tcactatgtt tataaatatt ttttgacatc ctcttgccat tcctctgtca atttctttac
    44461 tagaaatcca tctccaatta gcttcgcaaa tgttttagaa gactgataaa atgattcagc
    44521 taaactaaaa tctctttctt ctcttaatgc ttgcttccac aaaaacataa atacaagagg
    44581 tttcttttga taatcttgag ttttatccat aatttcttgt aaacacgata gataatcatt
    44641 aaaacaatca gttactccta ccttttcaag acaacacatt cctgaaataa gagtatctct
    44701 aattataaag aagttttcta atttagttac ctttttctgt tccaaaagaa agagcattag
    44761 cttttcgatt tgatttataa atttggcaag atcttttcta atgagcattt gataaaaaaa
    44821 caaacgaatc aacaacatat catttgttga ataaacttct ttttctatga tctgagacat
    44881 atgttcctca agtattaact ctgcatattc aggccttcta gttctagaag tatccaaaca
    44941 cgcctgcaat acttcacatg ctaattgttc ttctttagga agattatcaa agtatgtttt
    45001 ataaatctct tccaaacacg cttccttctt atcgtactcc tcttctttac catagattgg
    45061 ttcacgtaaa atctggtatt tcaattctaa ataagcagga ggaagagctg aaaaatccgg
    45121 cataaggcta taaactggaa ctcctagccg gcgagcaata taggctaact tgggcaaact
    45181 cggttgcgaa gctccacttt caattcgcga cagttgacga actgtcagtt cttgctcatc
    45241 tccacaaaaa tatggacgac taactccttt ttctatccga atctctttaa ttttttgacc
    45301 aattaacatc agacgcctcc tcttctacct gcttcgaaaa atactgtgaa acttaaaaaa
    45361 ttgtaacggc tacatctttc ctactcataa gatttcatag cttcttccac ttctgccaaa
    45421 gtaaccccaa acaactctag gcgtttgagg agttgcttgc cgttagaata gccgattcgg
    45481 agagtctctc cgagatattc tctacgctta cggctgtctg cccctgctag aaaaccaagg
    45541 cgaatcaagt cgctacgact aatgtcaaac tgactctcat gttcaaattg ttctgtcacc
    45601 tgagctagag ccgttttcag gtcttcatag ctggcatgct caattcccag agaacgcccc
    45661 ttggtcttgg actttggaac agcttcatct cgcttgagaa aggcatgccg aactgttgga
    45721 atggccgtca taatcatgcg ccgaatccgc tccccattaa aatctgggtc tgtaaagaca
    45781 atgactccct gacgttggtg caggcgctga atccgctcta tatcctgagc attgatggca
    45841 gaacctcgag tctcataggt ctccacatcg aaataacgtt tgagattgac cgtatcatca
    45901 cgcccttcaa ccacgataac ttgagaaatt ttctctttca ttacttgctg tccaatccaa
    45961 aaatgcgttc tgcatttgca gtcgttgcta ccgccagctc ttctgtcgtc ataccacgca
    46021 agtcagcgat aaagtcgacc acatagcgag tataggctgt tttattttca cgaccacgct
    46081 tgggtacagg tgctaagtaa ggcgcatctg tttccaccaa aatcttgtcc aaaggtaact
    46141 ctttagctgc ttcttggagg tcagttacct tcttaaaagt caccactcct gagaaggaaa
    46201 tggtcatacc aagatccaca aacttctctg cccactcaag cgtccctgaa aatgaatgca
    46261 tgataccacc acgaggacca acgccctcac tcttgataat ctcataggta tcttccagcg
    46321 catcacgggt atggacaaca aaaggcaaat ccaagtcctt agatagctga atctgacggc
    46381 gaaaaacctg ctcctgcacc tctttgggcg ctgtcatcca atggtaatct aagccaattt
    46441 cacctaaagc cacaaccttg ggatgtttta acttatccaa caagtaagcc tcaacttcct
    46501 ctgtataagt accagcttct gtaggatgcc aaccaatagt cgcatagagc tgatcatact
    46561 catctaccaa ctccaaggca cgctcaatcg tcggtttatc aaaaccaaca atattcatct
    46621 gtgtcacacc catctcagca gccaaggcaa tttcttctgc ctcacgacct gcaaattctt
    46681 ctacattcaa gtgtgtatgt gtatcaaaaa tcatctcttc taacctcgtt ttctatcttc
    46741 tattatacca aaaaagagga ggggcaccta atttttcggt ttcccctcct ctcttcaata
    46801 gagagctatt ctgctatctt ttctatccga tattgcccat ctcctattcc acagttagag
    46861 acagaagaga ttggctacat tgatagaata ttcctattca gctaaagtat gtgattatct
    46921 tgggttctca cttttctcac ccatcactag gattgatgac ttctcaaaat cccccttata
    46981 atctaacttt aagtagagaa tagaattttc tagctgttct attttctacg tctagtcttt
    47041 cttcctagca aagcaaaacc acttgttgcc accactagac caagactagc aagtgctgca
    47101 cttgatgctg aacctgtgtt aggtaattgt ttgccagcag gtgactggta ggtaacatct
    47161 tgcattgttc ctgcagttgc ttgcggtgca cttgttgtct taggagcagc attaggagcc
    47221 actggtctct taggaactgt aaccatgaca atggcttgca ctccaatcgt ttctgcttcc
    47281 ttcacatctt ccatagtttt cgcattttca atctctttga gagctgcttg tttttctgct
    47341 tccactcttg ctaaaagttc tgctttttct ttatcagaaa gcggtgcgcc tttgatttca
    47401 tcctgcttgt ccttggctgc tttttcaatg gcatttttag ctgattcttt ttcagtagct
    47461 aattgcttag aagcttgtga ttgagctgag ttttgtgaac ctgtgtgagc tgatccaccc
    47521 gcctggctac caccgcctgc attcgcatta gcatctgagc ttccagtatt gcctccctta
    47581 tctccacctg gtgtattttc tagcttatac tctggtgtag cttcttgagt tacagattca
    47641 cctttcgcag attgttgaac tgtatctttt cctagaatcg tcactactga accatctggg
    47701 aatgtgattg ttgctgtacc atctccagct acattgattg tcgctccgtc taatgctgaa
    47761 ccatttgctt gtaaaatagc aaccttaact ttttcttcat ctgttaaatg agttatatct
    47821 tttacatcta ctctttgagc aggtgcttta acagttgatt tttcagcagt cggtttagtg
    47881 ctatcttgtg cttttggatc ttgtgctacc aattgatttc ctggaattgt aatagctgaa
    47941 ccatctttaa agacgattgt cgctgcgcca tctccagcta cttcaacttt atagtaaaat
    48001 gaaataataa caggacaaat cgatcaggac agtcaaatcg atttctaaca atgttttaga
    48061 aatagaggtg tactattcta gtttcaatct actataacat cgcttccgaa tccatttgca
    48121 tctactgaac ttttttacaa aaataaaaat cagtagactc gagttcctac tgatttttga
    48181 tagaattcgt ttggattatg cagccaaaac tttgcgtcct ttacgacgac gagctgccaa
    48241 tacgcgacga ccatttttag ttgacatacg gttacggaat ccgtgtttgc gcgcacgacg
    48301 aagtttactt ggttgataag tacgtttcac gatgaatacc tcctcataga ttttgtattc
    48361 gtttagccgg ctataaagtg atcagttact aaacatactt tactattcta tctgattctt
    48421 tcacttttgt caatagctct aggcaaatgt ttccagcttt ttcgaatgca agcc
//
LOCUS       DAVBCB010000008        46516 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVBCB010000008 DAVBCB010000000
VERSION     DAVBCB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 46516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46516
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.008"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            24..143
                     /locus_tag="VQU28_000439"
     CDS             24..143
                     /locus_tag="VQU28_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HET2475857.1"
                     /translation="MIGIVFAIFLDFPVEYGISLGLLNGIVLGSLIVYKNNKN"
     gene            complement(649..1995)
                     /gene="gdhA"
                     /locus_tag="VQU28_000440"
     CDS             complement(649..1995)
                     /gene="gdhA"
                     /locus_tag="VQU28_000440"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HET2475858.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGTVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            2336..3070
                     /locus_tag="VQU28_000441"
                     /pseudo
     CDS             2336..3070
                     /locus_tag="VQU28_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3067..3285
                     /locus_tag="VQU28_000442"
     CDS             3067..3285
                     /locus_tag="VQU28_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2475859.1"
                     /translation="MSISPRFEALEQAIASKDLEKVREAFKKMNSTWTINESVVRDNS
                     TAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(3342..3626)
                     /locus_tag="VQU28_000443"
     CDS             complement(3342..3626)
                     /locus_tag="VQU28_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HET2475860.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(3680..4615)
                     /locus_tag="VQU28_000444"
     CDS             complement(3680..4615)
                     /locus_tag="VQU28_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HET2475861.1"
                     /translation="MEICQQILEKIKEYNTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQKILKQYNVTDAETAAIVGAPGRIDSVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            4719..5162
                     /locus_tag="VQU28_000445"
     CDS             4719..5162
                     /locus_tag="VQU28_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HET2475862.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELAAKVG"
     gene            5278..5544
                     /locus_tag="VQU28_000446"
     CDS             5278..5544
                     /locus_tag="VQU28_000446"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HET2475863.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSYILKRSRDYQDQNIK"
     gene            5541..5915
                     /gene="crcB"
                     /locus_tag="VQU28_000447"
     CDS             5541..5915
                     /gene="crcB"
                     /locus_tag="VQU28_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2475864.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYYLGRKKW"
     gene            5909..6238
                     /gene="crcB"
                     /locus_tag="VQU28_000448"
     CDS             5909..6238
                     /gene="crcB"
                     /locus_tag="VQU28_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HET2475865.1"
                     /translation="MVIVYLAIACGLGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            6357..6704
                     /gene="rplS"
                     /locus_tag="VQU28_000449"
     CDS             6357..6704
                     /gene="rplS"
                     /locus_tag="VQU28_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HET2475866.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            6747..6818
                     /locus_tag="VQU28_000450"
     tRNA            6747..6818
                     /locus_tag="VQU28_000450"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6780..6782,aa:Arg,seq:cct)
     gene            complement(6985..7983)
                     /locus_tag="VQU28_000451"
     CDS             complement(6985..7983)
                     /locus_tag="VQU28_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HET2475867.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDISMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(8026..8832)
                     /gene="yidA"
                     /locus_tag="VQU28_000452"
     CDS             complement(8026..8832)
                     /gene="yidA"
                     /locus_tag="VQU28_000452"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HET2475868.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRPMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(8845..10149)
                     /locus_tag="VQU28_000453"
     CDS             complement(8845..10149)
                     /locus_tag="VQU28_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HET2475869.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTVTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESQNPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            10221..10598
                     /locus_tag="VQU28_000454"
     CDS             10221..10598
                     /locus_tag="VQU28_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HET2475870.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELMMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            10689..11021
                     /locus_tag="VQU28_000455"
     CDS             10689..11021
                     /locus_tag="VQU28_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HET2475871.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            11033..12604
                     /gene="ffh"
                     /locus_tag="VQU28_000456"
     CDS             11033..12604
                     /gene="ffh"
                     /locus_tag="VQU28_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HET2475872.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(12799..14082)
                     /locus_tag="VQU28_000457"
     CDS             complement(12799..14082)
                     /locus_tag="VQU28_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HET2475873.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(14385..15098)
                     /gene="rsmG"
                     /locus_tag="VQU28_000458"
     CDS             complement(14385..15098)
                     /gene="rsmG"
                     /locus_tag="VQU28_000458"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HET2475874.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            15192..15752
                     /locus_tag="VQU28_000459"
     CDS             15192..15752
                     /locus_tag="VQU28_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HET2475875.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTSEEEKSVPKVDFSGLGD"
     gene            15754..16653
                     /gene="htpX"
                     /locus_tag="VQU28_000460"
     CDS             15754..16653
                     /gene="htpX"
                     /locus_tag="VQU28_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HET2475876.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(16697..18256)
                     /locus_tag="VQU28_000461"
     CDS             complement(16697..18256)
                     /locus_tag="VQU28_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2475877.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKTAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLAQELQLTVRGQEKIGIIGPNGVGKSTLLAKLQQLLNDKREISLGFMPQDYHK
                     KLQLDLSSIAYISKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(18495..18812)
                     /locus_tag="VQU28_000462"
     CDS             complement(18495..18812)
                     /locus_tag="VQU28_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2475878.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNDQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            18991..19533
                     /locus_tag="VQU28_000463"
     CDS             18991..19533
                     /locus_tag="VQU28_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HET2475879.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNQLDQEVLDDD
                     LVLPIENGELDLAESVSDNILLNIPIKVLTAEEEAGQGFISGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            19533..20162
                     /gene="nth"
                     /locus_tag="VQU28_000464"
     CDS             19533..20162
                     /gene="nth"
                     /locus_tag="VQU28_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HET2475880.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            20373..20894
                     /gene="pyrR"
                     /locus_tag="VQU28_000465"
     CDS             20373..20894
                     /gene="pyrR"
                     /locus_tag="VQU28_000465"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HET2475881.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            20913..21836
                     /locus_tag="VQU28_000466"
     CDS             20913..21836
                     /locus_tag="VQU28_000466"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HET2475882.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGKFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            21886..22965
                     /gene="carA"
                     /locus_tag="VQU28_000467"
     CDS             21886..22965
                     /gene="carA"
                     /locus_tag="VQU28_000467"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HET2475883.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            23277..26453
                     /gene="carB"
                     /locus_tag="VQU28_000468"
     CDS             23277..26453
                     /gene="carB"
                     /locus_tag="VQU28_000468"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HET2475884.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKERPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDQVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(26728..27537)
                     /locus_tag="VQU28_000469"
     CDS             complement(26728..27537)
                     /locus_tag="VQU28_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2475885.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDQFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNIPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
     gene            complement(27539..28342)
                     /locus_tag="VQU28_000470"
     CDS             complement(27539..28342)
                     /locus_tag="VQU28_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HET2475886.1"
                     /translation="MKQLTVEDAKQIELEILNYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPMDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRLVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSATFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(28352..29839)
                     /gene="tacF"
                     /locus_tag="VQU28_000471"
     CDS             complement(28352..29839)
                     /gene="tacF"
                     /locus_tag="VQU28_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HET2475887.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGDILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            30175..30882
                     /locus_tag="VQU28_000472"
     CDS             30175..30882
                     /locus_tag="VQU28_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HET2475888.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDSIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            30887..31909
                     /locus_tag="VQU28_000473"
     CDS             30887..31909
                     /locus_tag="VQU28_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HET2475889.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPEAEIVVIGRHWEKLELFSFAKECYITDNI
                     PEELAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFKNAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            31926..32795
                     /gene="licA"
                     /locus_tag="VQU28_000474"
     CDS             31926..32795
                     /gene="licA"
                     /locus_tag="VQU28_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HET2475890.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            32785..33663
                     /locus_tag="VQU28_000475"
     CDS             32785..33663
                     /locus_tag="VQU28_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2475891.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            33675..34364
                     /locus_tag="VQU28_000476"
     CDS             33675..34364
                     /locus_tag="VQU28_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HET2475892.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQNIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     HKLEEILKNEN"
     gene            34462..35310
                     /gene="dprA"
                     /locus_tag="VQU28_000477"
     CDS             34462..35310
                     /gene="dprA"
                     /locus_tag="VQU28_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HET2475893.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            36194..37237
                     /locus_tag="VQU28_000478"
     CDS             36194..37237
                     /locus_tag="VQU28_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HET2475894.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGHNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFYTESEEQ"
     gene            37404..39491
                     /gene="topA"
                     /locus_tag="VQU28_000479"
     CDS             37404..39491
                     /gene="topA"
                     /locus_tag="VQU28_000479"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HET2475895.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            39712..40071
                     /locus_tag="VQU28_000480"
     CDS             39712..40071
                     /locus_tag="VQU28_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HET2475896.1"
                     /translation="MRIIYLIIGFLSLTLAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            40164..40796
                     /locus_tag="VQU28_000481"
     CDS             40164..40796
                     /locus_tag="VQU28_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HET2475897.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            40840..41496
                     /locus_tag="VQU28_000482"
     CDS             40840..41496
                     /locus_tag="VQU28_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HET2475898.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            41515..42609
                     /locus_tag="VQU28_000483"
     CDS             41515..42609
                     /locus_tag="VQU28_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HET2475899.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     RALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            42621..43658
                     /gene="leuB"
                     /locus_tag="VQU28_000484"
                     /pseudo
     CDS             42621..43658
                     /gene="leuB"
                     /locus_tag="VQU28_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            43655..43924
                     /locus_tag="VQU28_000485"
     CDS             43655..43924
                     /locus_tag="VQU28_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HET2475900.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKSKARRRVWRIPEKILLIL
                     AFTFGGFGAWLAGIIFHHKTRKWYFKIVWFLGMVTTLVALYFIWR"
     gene            43928..44287
                     /gene="leuD"
                     /locus_tag="VQU28_000486"
     CDS             43928..44287
                     /gene="leuD"
                     /locus_tag="VQU28_000486"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HET2475901.1"
                     /translation="MAGSSREYAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLKPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNSLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            44449..45017
                     /locus_tag="VQU28_000487"
                     /pseudo
     CDS             44449..45017
                     /locus_tag="VQU28_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019771122.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            45111..45176
                     /locus_tag="VQU28_000488"
                     /pseudo
     CDS             45111..45176
                     /locus_tag="VQU28_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            45339..>46516
                     /locus_tag="VQU28_000489"
     CDS             45339..>46516
                     /locus_tag="VQU28_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HET2475902.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYTEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELMSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     "
BASE COUNT        14163 a         8319 c         9959 g        14075 t
ORIGIN      
        1 taacaatagc ctcactaggg gtgatgattg gaatagtgtt tgcaattttt ttagattttc
       61 cagttgaata tggtatttct ttaggcttgt tgaatggaat agtattgggt tcgctgattg
      121 tttacaaaaa caataagaat taagcataat tttttgctgt aaactaagga gtagagatgg
      181 ctatagttga aattataaat ctaacaaaaa gctttaaaga tattgaagtt attcataaca
      241 cttaaataat agagcaacta cagtagtagc ttaaaaacat gattaaatcg ctattcttag
      301 gagtagcggt ttttcttttt gtttaatact ctttgaaaat ctcttcaaac cacgtcagct
      361 ttgccttgcc gtactcaagt acagcctgcg gctcgcttcc tagtttgctc tttgattttc
      421 attgagtata aaaagggtta agtaagtata gtaaattgaa ataagatatg aacaaatcga
      481 ttagaaaagt caaattaatt tctagaagta tgttagaaat tggtttgaat tccgcaatca
      541 atttgttcag tttttatttc atttcatttt atttaattag attttccaat tttttaattc
      601 aagctaaaaa tccccaatcg tagtgattga ggattgagta aataaatctt aaacaatacc
      661 ttgtgcaatc atggcatttg ctacattttc aaaggcagca atgttagctc ctgcaaggta
      721 gtctttatca agaccgtatg tttctgaagt cgttttagct gtgttgaaga tgtttgtcat
      781 gatgtctttg agacggccat caacttcttc acgagtccat gagaggcgaa gactgttttg
      841 gctcatttca agagctgaaa cggctacacc accagcgttg gcagcttttg caggtccgta
      901 gaagatacca ttttctttgt aaactttgat ggcatcaagg tcgctcggca tgttggcacc
      961 ttcagataca cagataacgc cttgagcaac caaacgttta gctgcttcac cgttgatttc
     1021 gttttgagtg gcacatggaa gagcaatgtc atagtttcca gcgtaagtcc atacagtacc
     1081 ttcgtggtag gttgcagttg ctttttcagc tgcatactca gtcaaacgag cacgacgttt
     1141 ttctttaaca tcaaccaaaa gatcgaagtc gataccattt tcatcgatga cataaccatt
     1201 tgagtcagaa acagaaataa cagttgcacc gagttcagtt gctttttgaa gagcatattg
     1261 agcaacgtta ccagaacctg aaataacgac tttcttacca gcaaagctgt taccgttagc
     1321 tttgagcatt tcttcagtat agtaaaccaa accgtaacca gttgcttctg gacgaatcaa
     1381 gctaccacca aatccaagag gtttaccagt caagacacca gcatcaaatt ggttaagacg
     1441 tttgtattga ccgtaaaggt aaccaatttc acgtccacca acaccgatat caccagcagg
     1501 tacgtcaagt gatggtccga tgtgtttttg caattcagtc atgaagcttt ggcagaagcg
     1561 catcacttca gcatctgttt tacctttagg atcgaagtct gatccacctt tacctccacc
     1621 gataggaagt ccagtcaaga catttttaaa gatttgttca aatccgagga atttcaagat
     1681 cccttggttt acagttgggt ggaaacgaag tccacctttg tatggtccaa cagctgagtt
     1741 gaattgaaca cggtaaccac ggtttacttg aatttttcca tcacggtcaa cccaaggaac
     1801 acggaaagaa accacgcgct caggctcagt aatacgtgcc aagatatttt cttcgatata
     1861 ctcagggtgt ttttcaaata caggttctaa agtgttgaaa aattcttcaa cagcttggag
     1921 gaattcagcc tcgtgcccgt tacgagcttt cacagtttca aacacgcttt ggatatattc
     1981 tttagcagat gtcatatcgt tctcctttta ttgtcatatt ttattacata agacattata
     2041 gcagaatttt ttttcactgt aaagaaaaaa cagaaatttt ctaaaaattc tttcaattca
     2101 gtttttggtt tttttgaaaa tagataaaaa aacgaacgtt tttcttggaa atatctttct
     2161 caaagacaaa atcttaaagt atggtatgct ataagaaaag aaaatgttta gcaattcaga
     2221 aatcacttgt taatttatcc gtgatatgtt agatttgcta gtaaaattat gaccattttg
     2281 gaggaaagca atatttatac cagactaaaa tcaaaaatca ttatataata gtgatatgaa
     2341 atcaactaaa gaagaaatcc aagccatcaa aacactttta aaagactctc gtacagctaa
     2401 atatcataaa cgccttcaaa tcgttctata gtaaaatgaa acaagaacag gacaaatcga
     2461 ttaggacagt caaatcgatt tctaacaatg ttttagaagt agaggtgtac tattatagat
     2521 tcaatacact atagactgta atcaaacaac gatttggcga aatgtaaaaa atatgaggag
     2581 ttcggactcg actctctcct tcaagaaaca cgtggtggtc gtaaccatgc atatatgaca
     2641 gttgaggaag agaaagcctt tcttgcccgc catttgaagg ctgcagaggc aggagaattt
     2701 gttacaattg atgccttatt tcaggcttat aaaaaggagt gtggatgaac gcctttttag
     2761 aagagctttc acaagcttat ccagatgatt atcttttact cgttatggac aatgctatat
     2821 ggcataaatc aagtgcctta aagattccga ctaatattgg ctttgcattt attcctccat
     2881 acacaccaga gatgaaccca ttgaacaagt gtggaaagag attcgtaaac gtggatttaa
     2941 gaataaagcc ttttaaactt tggaagatgt catgaatcaa ctccaagatg ttatacaagg
     3001 actggagaag gaggtgataa agtccatcgt taatcggaga tggactagaa tgctttttga
     3061 aagcagatga gtataagtcc tcgttttgaa gccttggaac aggccattgc ctccaaagat
     3121 ttggaaaaag tccgagaagc ttttaagaaa atgaattcca cttggaccat caatgaaagt
     3181 gtggttcgtg acaatagtac agctcattat ggacgtgttg aaacagctat ttccttcctg
     3241 cctagtagca tggaaaccga acctacagat gagtctggaa catgaaaaca gcagttgatt
     3301 tcaagaaaca aaaaagaaca gtcgaaactg ttcttttatc attatttgag accgtatttt
     3361 ttgttgaaac gatccacgcg tccatctgct tgagtgaact tttgacgtcc agtgtagaat
     3421 gggtgtgagt ctgatgaaat ttccacacgg atcaatgggt aagtttcgcc ttcgaactca
     3481 actgtttcgt tagagcgttt tgttgaaccg ctaaggaatt ggtaaccagt agttgtgtcc
     3541 atgaagacaa ctgggcgata ttctggatgg atatcttttt tcattttgca atatttcctt
     3601 tctgccatgg tctctttgcg agccatagat tgttaccata ctagtctatc agattctgaa
     3661 aagtttggca agtattttat cagtttttaa gcaagttttt taacttttgg tagatgattt
     3721 cattttcttc taggctatag gaattagcac cacttgctag agggtggcct ccaccatcat
     3781 gctccttggc gatttcattg ataggatgga ttttactgcg taagcgaacg cggtagtggc
     3841 catcagcctg ttccacaaaa attccccaga gactaacact atcaatacgc ccaggtgcac
     3901 ctacaatggc cgcagtttca gcatcggtta cattgtattg tttcaagatt ttctgactca
     3961 ggataacgcg agcagcacca ttttcatcca cttccagatg gtcgtagatg tagccttgca
     4021 gtttagcaat tttgtagctc atagtgtcca ttttgcgagt gagagccgca aagtcaaagt
     4081 tatgttctct caaataagca gccaggcgaa gagtccgtgc agtggtagaa gggtagagga
     4141 agcgacctgt atcaccgaca attcctgcaa agagcaactc agcatcgcga tctgccaagg
     4201 ctagttgggt tgtttgggca aatagggtaa tcatctcgct agcgctactt gaactagtat
     4261 cgacccaaga caggtcaccg tatacatcat catttggatg gtggtcaatc ttaatgagaa
     4321 aatcaccttg actatagcgc ttatcatcga tacgagcagt attagctgta tcacagacga
     4381 tgacaagtgc gccttggtag gctctatctt caacaagatc catctcagcc atccaagtaa
     4441 gagttggttc atcaaaaccg acggctttga tggttttttc tgggaaatga tgttccagca
     4501 aggctttcaa tcccacctga cttcccaagg catcagggtc tggtttcata tgacgatgaa
     4561 tgataatggt gttgtattct ttaatttttt ctaaaatttg ttggcaaatc tccataaata
     4621 acctcaattc tttctcattt cattgtagca caagaatttt tatttttaaa atgaaaaaga
     4681 tgtgatataa tggagaaaga taaattgagg aggacgatat ggcattagca aaaattgtat
     4741 ttgccagtat gaccggtaat accgaagaaa ttgcagatat tgtagcagac aaattacgtg
     4801 acttgggctt ggatgttgat gttgatgaat gtacaactgt tgacgcttca gacttcttgg
     4861 aagcagacat cgctatcgtt gcgacctata cttatggtga tggtgaattg ccagatgaga
     4921 tgatggactt ctacgaagac ctagcagatc tcaacttgaa tggcaaaatc tacggagtgg
     4981 tcggctcagg agataccttc tacgatgaat tctgtaaggc tgttgatgac tttgaccgtg
     5041 tctttgtgtc aacaggagca gaaaaaggtt cagagtgtgt taaagttgat ctttctgccg
     5101 aggaagaaga tattgaacgc ttggaacaat tcgcagaaga attggctgct aaagtaggat
     5161 aagcataaaa gtgcgctgat gtaatcaatc agtgcatttt tcttatcgag agttgggatt
     5221 ttactagcct aattgatggc tttttgatac aataattcaa ataaaggagg agactgtatg
     5281 gatttagata ttattcgtca agaaatcgat caaatcgacg accaaattgt caaactgtta
     5341 gaagaacgaa tgcatttagt tgagggggtg gttgcttata agaaagcttc aggtaaaccg
     5401 attttagata ctaagagaga agaagttatt tttgaaaagg ttaggagtcg tgtagaggac
     5461 aagcgctatc aggagactat tgtcgcgact ttttcgtaca tactcaaacg ttcgcgtgat
     5521 tatcaggatc aaaatatcaa atgaaaaaag aacaatttta tccgctagga atttttctag
     5581 ctgctatgtt gggcggactt gtccgatatc tagtttccac ctggttacca gccagtccag
     5641 actttccttg gggcactctc tttgtcaact atctgggaat tttctgcttg atttttcttg
     5701 tcaagggcta tctggtctat aaggggacta gtaagggctt gattttagca ctggggacgg
     5761 gtttttgcgg aggtttaaca actttttcca gcctaatgct tgatactgtg aagctgcttg
     5821 atacagggcg ttattttagt ttagtcctgt atttgctttt gagtatcggt ggaggcctgc
     5881 ttttagctta ctatttgggg aggaagaaat ggtaatcgtc tatcttgcaa tcgcctgtgg
     5941 cttgggagca ctcgtacggt atttcttttc tcgctataat caagcttcta aattgccact
     6001 cggaacgctc atagctaatc ttttaggttg ttttttaatt ggagtattct acaatcatgt
     6061 agagtctaag gaagtatatg ctattctagc aacaggattt tgtggcggtt taacaacttt
     6121 ttcgaccttg aatgacgaac ttcaaagact gctaagtgat aagaaggtct tttattctta
     6181 cctgactttg acttacatag gtggtttggt tgcgattttt ttaggaattt tgctataaat
     6241 ttctttactt ttttttagaa atttggtaaa ctatatcttg tgtgtaatgg acgcacaaaa
     6301 atacagttta tccgctgagg aagattcctc aagattgaca aagataggag aataaaatga
     6361 atccattaat ccaaagcttg actgaaggtc aacttcgtac agatatccca tcattccgtc
     6421 ctggtgacac tgttcgtgta catgcgaaag ttgtcgaagg taaccgtgaa cgtatccaga
     6481 tttttgaagg tgttgttatt gcacgtaaag gtgctggcat ctctgaaaac tacacagttc
     6541 gtaaaatctc taacggtgta ggtgttgagc gtatcttccc aatccacact ccacgtgttg
     6601 aaaaaatcga agttgtacgt tacggtaaag tacgtcgtgc gaaattgtac tacttgcgtg
     6661 ctcttcaagg taaggcagct cgtatcaaag aaatccgtcg ttaattcgac taaaaaactc
     6721 tgctttttca gcagagtttt tatctagctc ccttagttca atggatataa caactccctc
     6781 ctaaggagta gttgcaggtt cgattcctgc agggggcatg taattagata tcaaagaagt
     6841 ttgtcaaaga gtctttgata ttaaaagaac gcataatata ggggattgtc tccataatat
     6901 tatgcgtttt attattgaaa aatttgggca atcatcagat aaaattgaga ctatatcgag
     6961 tatataaatg tttgtatagt caatttattc aaaatcgtca agttctaaat tatttaaagg
     7021 actataacat tcatatttct ttaagtaatt gtttatttct tcagcagtgg aacgaggtaa
     7081 attaactctt aaatagtcaa tatcttcctt agataaaaaa tgatttgcat tcaaaattga
     7141 gaagcgaaat aatgcttcat caaaagcaaa atcataatcg tcatcagata atgattccat
     7201 taatgtttga atatgtttta gtgagtagtc aggtatcgaa tcgggataat atactggata
     7261 atcagtggct gaaaaattgt tttctaatcc tgaaatagta attatcaaag attctatgta
     7321 atatattaaa gcatgtttaa tattaccttc ttgttccaaa tgtcttccct gagcctttcg
     7381 tgtgttagat aagcatccaa aatctcctga tacagtattc ctatgagctt gtttatttaa
     7441 gagactccat tttatatcac caaaagttgc tttaaaagag atattgtttt tttctttaca
     7501 ataatcttcc atacttatgt ctttatcata gtatgctttg atatagtcac tatgttcgat
     7561 aataagattt tgtgcttctt ttgttagttt aaattcgaac ggaatatcta aattgtcaat
     7621 gttaacatta ttaattattc ttgtaattaa ttctgctttt tttccagaag tagctaaatt
     7681 atgtttagat aatatagttt ttaattctac tactgtaaga tatgataaag tcttattttt
     7741 agtgaagtat cctaatttgt ataattgatt gtgtgtagat tcagggtcaa tttgaagaga
     7801 gtatttaaaa tatcttggga aattataatt tctccctgtt ttttgagtcc aatctatgag
     7861 tatgagttgc ccaggagtaa ggccacattc taattcgttt aggtaaaaat tgttacaatc
     7921 agaatttgtg tctctattat gattattttg ttttaaatta aatagtttgc taaaaaaatt
     7981 cattggattt tattgctaat caattcttat tgaaaaatga tacttttaca gtacccatgt
     8041 tcggatggca tgggcaacgc cggattcgtc atttgttttg gtgatgtatt tggcgatttt
     8101 tttgatttct ggatttccat tttccatgac aacggggttt ccaacgactt ccagcatggg
     8161 acggtcattt tcttcatcac cgattgccat ggtttcatct ttggtcaatc cgagtttttc
     8221 agccaagtga gtaatggctg aacccttgtc tacattcttt ttaaggagtt cgaggtagaa
     8281 aggagcagat ttgttgatgg agtagcgctc gtaaaattct gctggaattt tttcaatcgc
     8341 agcatcgaga atttctggtt catcgataaa catacattta acaatttctt tgccagccat
     8401 ttcttcaggg gtacggtaga agataggcat gctgacgagg gttgattcgt gtacagtgta
     8461 ttttccgata ttgcgatttg cagtatagat accgtccttg gtaatggcat gcatgtggac
     8521 accgagcttg cgactgagga attccatatc cagataatcc tcataggtca aggattcgct
     8581 gataatctca tggccagtag cagtttcttg gacaagggca ccattgaagg tcaccacata
     8641 gtcaccttgg tctctcaact gcaagtcgtc cagaagtttg gcaacacctg cgatggggcg
     8701 gccagttgca atcacgactt tgacaccagc ttctttggca tcttggatgg cagaaaagac
     8761 ttcaggagtg atttcctttt ggctgttgac tagggttccg tcaatatcga cggcaattag
     8821 tttaatactc ataaaattcc tcttctagtt tttatttggg gtaaaatgat cgttctcaat
     8881 caagtgtaaa aattgctggg taatgcttgc gaagatgctg ttttggtcta acatttcttt
     8941 agggaaatag aaacgattat ctccgtggcg actgccagca agggattgga cgataggaga
     9001 caggctagag agttcggcca gttctccatt tttttgtaaa atctcaatct gtgtccgtgg
     9061 attttgagat tcgggacgat agatatcata agggaggtca aagttcttat gaatggcagt
     9121 atagtagtcg ggatcaaagc cgatgtcctc aaccaatttt ctcatgctag taagttgatc
     9181 ttggtcctct tgtgaaaagg taatggattt aaagaccttg cggttgacaa agcgttgaga
     9241 taaatctgca agaattttgt caggactagt catccaaagc tggaagtagg tattcatcac
     9301 gccatcatcc agagccagat agtcagtcaa ggtcacattt ttttcgaaga aaggcaggag
     9361 atgtggggaa gttcgtgcaa agaaattctt gtcctcaggg tagagttcct tggcgcgctt
     9421 gagaagattc tgtaggagaa cttccatggc gcgtgttgct gggtggaaat aaacctgcat
     9481 gtacatctgg tagcgactga ggacgtagtc ttcgatggcg tgcatgccat tgcgctgaaa
     9541 ggcgatacca ttttcgacag gacgaatgac tcggaggatg cgagtcaggt caaattcccc
     9601 ataggatgct cctgtaaaat aggagtcgcg caagagatag tccatgcgat ctgcgtcaat
     9661 ctgactagaa atgagctgca cgacctgctt attaggatag gtatggtcaa tgacactggc
     9721 caccttttct gggaaatcag gtgccacttg tagcaggact tggtgaatct ctgtctcagg
     9781 attttgaata atctcctgag taatggcttc atggtctgta tcaaagagat tttcaaaagt
     9841 atgggagtag gcaccatgcc caaggtcgtg taggagagca gcggtcacgg tcaagagaga
     9901 ctcggcagga ttccattcct caggatattt ttcttcgaaa atctctgtga tgcgtcgtgc
     9961 aatttcatag actcctagac agtgagagaa gcgactgtgt tctccaccgt ggaaggtata
    10021 actggaagtt cccagttgtt tgatccggcg caaacgctga aattcttttg tattaatcaa
    10081 gtcatagatg atttgattat tgacatggat gtagttgtga actgggtcac ggaatacttt
    10141 ttcgttcata tgaccattat agcaaaatcc tgctcttttt ggtaaaatag taggacaaga
    10201 gacaagaaag agggcctgat gtgaagattc ggatgcgaaa tacaattcag tttgatgagc
    10261 agttggaagt gattgaccag ctttatgatg tggaagtgca tgaaaaagga gattatagct
    10321 acctgctttt ctataatgag gaaaaggaaa aagtagtgat taaatttcat ggtcaagaac
    10381 ttatgatgag ccgattttct aatcccaaga ccattatgcg ctttctaaag gatagcgata
    10441 gtttagccta tattcctaca cctatgggca tgcaggagtt tatcatccaa acgagccatt
    10501 atcaagttga taggcaaaag attgaactag attatcaact acaaaatcaa gagggacatc
    10561 cttttgccag ctatcaattg gaaattactt ggggctaggc tcatgtcttt ttcaaattta
    10621 gctatctatc ttcttggatc ggcttcctag tgtggtagat taggctaatt tttggtataa
    10681 taaaagttat ggaaatcgaa aaaaccaatc gtatgaatgc gctctttgaa ttttatgcgg
    10741 cgcttttgac agataagcaa atgaattata tcgagctcta ctacgctgat gattacagcc
    10801 ttgctgaaat tgccgaggag tttggtgtca gtcgtcaggc tgtctatgac aatatcaagc
    10861 gaacagaaaa gattctggaa gattatgaga tgaaattgca catgtactcg gactatattg
    10921 tccgcagtca gatttttgat cagattttgg agcgctatcc caaggatgac tttctgcagg
    10981 agcagataga aattttaaca agcattgata atagagaata agaggaagaa aaatggcatt
    11041 tgaaagttta acagaacgtt tgcagaacgt ctttaaaaat ctacgtaaaa aaggaaaaat
    11101 ctctgaatct gatgtccaag aggcaaccaa agaaattcgc ttggccttgc tcgaggccga
    11161 cgttgccttg cctgttgtaa aggactttat caagaaagtt cgtgagcgtg cagtcgggca
    11221 tgaggtcatt gatacactta atcctgcgca acagattatt aaaatcgttg atgaggaact
    11281 gacagccgtt ttaggttctg atacggcaga aattatcaag tcacctaaga ttccaaccat
    11341 catcatgatg gttggtttac aaggggctgg taaaacaacc tttgctggta aattggccaa
    11401 caaactcaag aaagaagaaa atgctcgtcc tttgatgatt gcggcggata tttatcgtcc
    11461 agctgccatt gaccagctta agaccttggg acaacagatt gatgtgcctg tctttgcact
    11521 tggaacagaa gtaccagctg ttgagattgt acgtcaaggt ttggagcaag cccaaactaa
    11581 tcataacgac tatgtcttga ttgatactgc gggtcgtttg cagattgatg agctcctcat
    11641 gaatgagctt cgtgatgtga aagcattggc tcaaccaaat gaaatcttgc ttgtcgttga
    11701 tgctatgatt ggtcaggaag cagccaatgt tgcgcgtgag tttaatgctc agttggaagt
    11761 gactggggtc atccttacca agattgatgg cgatactcgt ggtggtgctg ctctgtctgt
    11821 tcgtcacatt actggaaaac caatcaagtt cactggtaca ggtgaaaaga ttacggacat
    11881 tgaaaccttc cacccagacc gcatgtctag ccgtatcctt ggtatggggg atatgctcac
    11941 tttgattgag aaagcttctc aggaatacga tgaacaaaaa gcccttgaaa tggctgagaa
    12001 gatgcgcgaa aacacctttg attttaatga tttcatcgat caattagatc aggtgcaaaa
    12061 tatggggccg atggaagact tgctcaagat gattccaggt atggccaaca atccagccct
    12121 tcaaaacatg aaggtggatg aacgccagat tgctcgtaaa cgtgccattg tgtcttcgat
    12181 gacacctgaa gagcgtgaaa acccagattt gttaaatcca agccgtcgcc gtcgtattgc
    12241 tgctggttct ggaaatacat tcgtcgaagt caataaattc atcaaggact ttaaccaggc
    12301 taaacagctc atgcagggtg ttatgtctgg ggatatgaat aaaatgatga agcaaatggg
    12361 gattaatcca aataaccttc ctaaaaatat gccaaatatg ggaggaatgg atatgtctgc
    12421 ccttgaagga atgatgggac aaggcggtat gcctgactta tcagctctcg gaggagcagg
    12481 aatgccagat atgagccaga tgtttggtgg cggtttgaaa ggtaaaattg gtgaatttgc
    12541 catgaaacag tccatgaaac gtatggctaa caaaatgaag aaagcgaaga agaaacgcaa
    12601 gtaagacaag agattgatgc aaatctcatt tgatgagaaa ataaagtaaa aatttctatg
    12661 ataaaacgta cagtatcaag gttttagaat atctgatatt atacgttttt tgattttaat
    12721 aattttgtta agacactttt attatggtat acgaaaaatc cactcagctg tctgagtgga
    12781 ttaggtgtat ttagactctt agtctttatt ttcgtatggc aagatcaagt tcaagatgat
    12841 tcctgtcatg gctgaaaggg cagtacctga aagtgtaact ggaccaagtt taaggatagc
    12901 tcctccaagt ccaagaacca acatagcact tgcgatgatg aggtttcgca tttgagcgaa
    12961 atcaacacgt tctttaatca agactttcaa accattgctg gcgataaccc catagagaag
    13021 gattgacata ccaccaagta cagcgtttgg aatagttgaa atcaaggcag tgaatttacc
    13081 aaggaagctg agggcaatcg cgatgaaggc agcgttacgg ataactgaga cagaagcgat
    13141 acgagtcata ccgataaccc ctgtattttc tccgtaagtt gtattggctg gtccaccaag
    13201 gaaggcagaa acagaagttg cgataccgtc accaagaaga gtacggtgaa gacctggttc
    13261 ttttaagaat tgacgaccac agatttgacc caaaacagta tggtctccga tatgttcaga
    13321 aattgttacg atagcgattg gcaagatagc gatggcttct ggaccaaagt aaagattgta
    13381 ctctttaaag gcaccacctg tgctaaatgg caagtagaaa ccaggaattt cgaaccagtt
    13441 ggctttaaga actggtgtaa agtcaaccaa gccaagagtt agtgcgaaaa ggtaaccacc
    13501 gataatggca aagaggaatg gaatgattcg taggaagcct tttccttttg tattgataaa
    13561 ggcagcaatt aggaaagtaa caacggctac cagagcattt ttccaatttc cgtctgctac
    13621 aagacctgca ttggtaacag ctgaacctgc aagtccaaga ccgataacga tgatcatagg
    13681 accgataatg attggtggca agagtttatc aatccatttt gttcctacaa atcggatgct
    13741 ggtagcaaca aggacataga ccaaaccagt caagataacc cctgtttggg cagcagatac
    13801 atcccccccc atttctttca tagccagtga catagctgtg ataaaggcaa atgaagaacc
    13861 tagataaact ggaactttaa aaccagtagc aatcatgtag atgagtgttc caacacctga
    13921 agcaaaaagg gcaacagata caggcattcc caaaatcaat ggtaccaaga tggtcgcacc
    13981 aaacatggcg aaaacgtgtt ggaaactaag gagaattcct tttccagccg aaggacgttg
    14041 gtcaacgtct agtaacaaat caacagttga ttcttgtttc atataaacct cacttttggg
    14101 catcaaaaaa gagcccatta ttttacagca ataggccctc agagtaagac ttctttaaaa
    14161 tttagatttt aaagagtttt aaatatttct gcgtcttcgt cacctcacgg gatgacatta
    14221 aaaacctttg cttaggatag tatagcagaa aaaaatgatt ttgtaaatca ttttttcccg
    14281 agcctagaaa taaaagagcg aggctgattt tgtaaatcat tttttcccga gcctagaaat
    14341 aaaagagcga ggctgatttt gtaaacattt gtttactaaa aaatttaaag tgggcgttta
    14401 tttggcatac cagccttacg cggatattta tttggtgttt cttttttctt ttctaccact
    14461 gtgatatagc gcggatctct attcggtagg gcgtagctga gattgtcttc gaccttacta
    14521 aaaaggagat tgagggcatt cttagcttct aataattcct caggcgcatt gctggccttg
    14581 agtgccaata gttttccacc aaccttaagg tagggaatgg tcaattcaga taggacctgc
    14641 atacgggcaa ccgcacgagc tgttacaaaa tcatattgag cacggaagtt cttgtcttgg
    14701 gcaaaatctt cggcacgtcc gtggtagaaa tgaactccgt tcaaatccag ttcttgagcc
    14761 aagagttgta ggaagttgat gcgcttattg agtgaatcaa taatggtcac atctaactcc
    14821 ggatagagaa ttttcattgg tagactagga aatcctgccc cagccccgat atcaagaagt
    14881 ttgatagttt cattgggaat caaaccttga agaatgggtg caatcgaatc gtaaaaatgt
    14941 ttgagataaa cttcttcctt gtccgtaatc gccgtcaaat taatcttctc attccactcg
    15001 accaagagct caaaataacg ttcaaattgt tctttttgct ggtccgaaag tggaagattc
    15061 tgctcggcaa gcaagttgta aaatgttttt ggtttcatag ttatttcctt ctttagtatc
    15121 atcttatttt accacaatca tagaaaattt gatatactgg tactaatcta aaagcagaaa
    15181 ggacttatat tatgacttgg attattcttg gagttatcgc tcttattgtt atttttgtga
    15241 ttgttagcta taacggtttg gttaaaaatc gtatgcaaac caaggaggct tggagtcaga
    15301 ttgatgttca gttgaaacgt cgaaatgacc tcttgccaaa cttgattgag actgtaaaag
    15361 gttatgccaa atatgaaggt tctacccttg aaaaggtggc agaactacgt aaccaagtgg
    15421 cggcagcgac ttcaccagca gaagctatga aagccagtga tgccctcact cgtcaggttt
    15481 caggtatttt tgcagttgca gaaagctatc cagatttgaa agctagtgct aactttgtta
    15541 aattgcaaga ggagttgaca aacacagaaa ataaaatttc ttactctcgt caactctata
    15601 acagtgttgt cagcaactac aatgtaaaat tagaaacttt cccgagcaat attatcgctg
    15661 gaatgtttgg atttaaagcg gcagatttcc ttcaaacatc tgaagaggaa aagtcggttc
    15721 ctaaagttga ttttagcggt ttaggtgact aagatgttgt ttgatcaaat tgcaagcaat
    15781 aaacgaaaaa cctggatttt gttgctggta tttttcctac tcttagctct tgttggttat
    15841 gcggttggtt atctctttat aagatctgga cttggtggtt tggttatcgc actgattatc
    15901 ggctttatct acgctttgtc tatgattttt caatcgacag agattgtcat gtccatgaat
    15961 ggagcgcgtg aggtggatga gcaaacggca ccagacctct accatgtagt ggaagatatg
    16021 gctctggtcg ctcagattcc tatgccccgt gttttcatca ttgatgatcc agccttaaat
    16081 gcctttgcga caggttctaa tcctcaaaat gcggctgttg ctgcgacttc aggtctacta
    16141 gctatcatga atcgtgaaga actagaagct gttatgggac atgaagtcag tcatattcgt
    16201 aattatgata tccgtatttc gactattgca gttgcccttg ctagtgctat caccatgctt
    16261 tctagtatgg caggtcgtat gatgtggtgg ggtggagcag gtcgcagacg aagtgatgat
    16321 gaccgagatg gaaatggtct tgaaatcatt atgctagtgg tttccctact agctattgta
    16381 ctggcacctc tcgctgcaac cttggttcag ctcgctattt ctcgtcagag ggaatttctg
    16441 gcagatgcat ctagtgtcga gctgactcgc aatccccagg gaatgattaa tgccctagat
    16501 aagttggaca atagcaaacc tatgagtcgc cacgtcgatg atgctagcag tgccctttat
    16561 atcaatgatc ctaagaaagg tggggggttc caaaaactct tttataccca cccacctatc
    16621 tcagaacgga ttgaacgttt aaaacagatg taaaagaatc cagaggtcgt ctctggattt
    16681 ttgctatgtt gcaaatttat aaatcttcta aattaactag cttcaaaccg tgttctgtca
    16741 tgcgatagat gatcgagcag acttctttta agaaacgacg gtcatgcgaa acagtgatga
    16801 gaccgcctgg ataggttgca aagagttttc tgatttgggg ttgagaagtg ggagaaaagt
    16861 ttcgtgtggg ttcatccagc aggagaaagt ttggtttgcg cagaactaga tccaaaagca
    16921 ggagttttcc ctgttgtccg ccagataagg agcgaatttg atgctgcatt tctggataac
    16981 tgaaattgag actggctagg tgagattgga ttttctgaag ttcctctttt tccccagttt
    17041 tgctgatata agctattgag gataaatcca attgcagttt tttgtggtaa tcttgtggca
    17101 taaaaccaag tgaaatctct cttttatcat tcagaagttg ctgtaatttg gctaacagag
    17161 ttgatttccc aacaccattt gggccgatga taccgatttt ttcttggcca cggacagtta
    17221 gttgcagttc ctgagccaaa acacggtcgt caatggacaa attttctttt tccagttgga
    17281 ctaagacttt agaagctggt aatggttgga tgtctgaaaa gaaaagttgg atttgttcct
    17341 cttcaagtgg cttttgagtc atggactgag ctgtcttttc gtagcgtttt tcttgtgaga
    17401 ggacagtttt catcttttta gccaataggc gaccggcagt actatctttg gtagctcgaa
    17461 gcgcagtttc tacattttgc ttaactctcc gatgtttttc catggtttta tcgtaggctc
    17521 tttggttgtt agcagcttgc tgactttgtt tggcaaaatt agcctttctc tgctcactat
    17581 agctatcata gtctaaatgc tctactagcg tttccgcttc tttacggtgt ttgaccagtc
    17641 gcaagtgaac aatagtgtct gccgtttcag aaagaaagtc ttcatcatgg gaaatgaaaa
    17701 taacggtttg cctggtcttt tgaatctggc cttttagcca atcaactgtc tcaaggtcta
    17761 ggtcatttga aggttcatct aaaaatagaa tctcaaaggg tttggctaac tcatggataa
    17821 gctgaatttt caaagcttcg ccccctgata gattgccaat ctcttggtca cttgcgaaac
    17881 gattgctatc aaaatgcaat tcctccgcca aacgatagag gatactgtag tctaaatcaa
    17941 tagaatctaa aaagaagtag tcgtgtaaag ttttcttttt taggtcctca gggacttttt
    18001 gaggaatgta ggccagtgac tgataatcag actggatgtc tcccttgata gtgaaatcag
    18061 acaaatcttc ccccattaaa gttttaagta aggttgattt accatttcct tcttcgccaa
    18121 taatagctac cttttccccg tcttgtatgg tcatgcttag gtcagagaca aggtctcgta
    18181 aatctttgtt ttgtgtgatg gtcaggtgat tgatttttat catactattg ccctttctag
    18241 aatgtcgata gtgcataaca aaaagctcta ctttacctag tagagcccaa agtctatgtc
    18301 aaaaaaactc tattaacatg ccagaaaaag agcataaaag agcacacaaa aagagacaaa
    18361 aacaagatct actaaataaa ggttctttat ttgatagact tagttgttca tgtctccctt
    18421 acctccgagt attagtatct ttatactata cttttaagga aattttgtca acagaaaact
    18481 ggaaattcta tgctctaaaa tccaaaaaca ggtccataga gacttagtac gtgtttgaca
    18541 tgctcgtaat ggattttatc atagtttcgc caagcagtgc gtgcttgatc gtttagtttt
    18601 aggctaccca gtccaatcag aggactggtg ggttggtaaa atttctcaag atcaattaag
    18661 atactgttgt tcaacttttc cgtttttttg agctcttgca tagtagtgtt tagcagctct
    18721 tgtagtcgaa tatcatctgc cgtaccctgt ttacagtttt ggtagctttt atttaacttg
    18781 gataggagtt gaatgagttt cttgtcttcc atgtcttcca tatgagcatc ctccttgatc
    18841 tgatgtactc tttccctata gtatagcact aaaaaataaa actgtcatag taaatatgtt
    18901 aaaattttga attttaatta caaagtttgg tgtgctgatt tgtagccttt tcatggtata
    18961 atcaagagag taaatcttta tggaggaagt atgaaattaa atattcaaga aattcgtaag
    19021 cagtctgaag gtttgaactt tgaacaaacg ttagatttag ttgatgacct gcgtgcacgt
    19081 aatcaagaaa ttttagatgt aaaagatatc cttgcagttg ggaaagtaca atatgaagac
    19141 cgtatgtatt tcttagatta tcaactatct tataccattg ttcttgcttc gagtcgcagt
    19201 atggagccag ttgagttagt tgaatcttat ccagtcacgg aagttttcat ggaaggcgca
    19261 actaaccagc tagatcaaga agttttagat gatgacttgg tcttgcccat tgaaaatggg
    19321 gagcttgacc ttgctgagag tgtatcagac aatatcctgc taaacattcc tatcaaggtc
    19381 ttgacggctg aagaagaagc tggtcaagga tttatctcag gaaatgactg gcaaatcatg
    19441 acagaggaag aataccaagc tcaacaagca gtaaagaaag aagaaaacag tccttttgct
    19501 ggcttacaag gactatttga cggagatgaa taatggtctt gtcaaaaaaa cgagcacgaa
    19561 aggtgctaga agaaatcatt gccctcttcc cagatgccaa gcctagtctt gattttacca
    19621 atcattttga actcctggtt gcggtcatgt tatcagccca gacgacagat gcagcggtaa
    19681 ataaggccac accaggtctc tttgttgcct ttccaacacc ccaagctatg tctgtagcga
    19741 cagagagtga gattgcttca catatttctc gcttgggatt gtatcggaat aaagctaaat
    19801 tccttaaaaa atgtgcccaa cagttactag acgattttga tggtcaagtc cctcagacac
    19861 gtgaagaatt ggagagtttg gcaggtgttg gtcgcaagac agccaatgtt gtcatgagtg
    19921 taggatttgg gattccagcc tttgcagtgg atactcatgt ggagcgtatt tgcaaacacc
    19981 acgatattgt caaaaaatca gcgacgccac ttgaggtgga aaagcgggtc atggatatct
    20041 tgccgcctga gcagtggtta gctgcccatc aggccatgat ttattttgga agagccattt
    20101 gccatccaaa aaatccagag tgtgaccagt acccacaatt atatgatttt agcaatttgt
    20161 aagatgctac attgaaactt tttgcttgat tttaagcatg ctttgtgcta tagtaaatga
    20221 taactaaata ttcctttaaa cctgtcccgt gaggcaggca aggagcgcga taaagtagaa
    20281 gggtgaagtc tatactgttt gcagtagggc tcgcttggct ataaatttca agtctccttg
    20341 tttaaggaga cttttctttt tggaggtttg tcatgaaaac aaaagaagtt gtagacgaat
    20401 tgaccgtcaa acgagcgatt acgcgtatta cttatgagat tatcgaacgc aacaaagatt
    20461 tgaataagat cgtcttggct ggtattaaaa ctcgtggtgt ctttattgcc caccgaatcc
    20521 aagaacgttt gaagcagtta gaaaatcttt cagttcctgt tgtggaattg gatactaaac
    20581 ctttccgtga tgatgttaaa agtggagaag atacttcttt ggtttctgtc gatgtgacag
    20641 accgcgaagt tatcttggtg gatgatgtgc tctatacagg tcgtaccatc cgtgctgcta
    20701 ttgataatat tgtcggtcat ggtcgtcctg cgcgtgtgag tttagcagtt ctagtcgatc
    20761 gtggacatag agaattgcca atccgtccag attacgttgg aaaaaatatc ccaaccagtc
    20821 gttctgaaga aatcatcgta gagatgacag aacttgatga ccaagacaga gttctgatta
    20881 ctgaagaagc ttagaaagtt aaaggagtag ccatgtcaga aaatcaacaa gcattgaacc
    20941 atgtggtgtc catggaagac ctcactgtcg atcaagtaat gaaattgatc aagcgaggaa
    21001 ttgagtttaa aaatggagcc cagcttccct atgaagacca tccgattgtt tccaatctct
    21061 tctttgagga ttctacacgg acacataagt cctttgaagt cgcagagatt aaacttggat
    21121 tggaacgact tgactttgat gtgaagacta gttcggttaa taagggtgag acactttatg
    21181 acaccatttt gactctgtct gctttaggag tggatgtctg tgtgattcgc catccagagg
    21241 tcgactacta tagagaattg attgctagtc caacgattac gacttccatc atcaatggtg
    21301 gagatggttc gggccaacac cctagccaga gcttgcttga tttgatgacc atttatgagg
    21361 aatttggcca ctttgagggt cttaaagttg ctattgcagg tgacttggac cactcacgcg
    21421 ttgccaaatc caatatgcag attttgaaac gcttgggagc tgaactcttt ttcgctggac
    21481 ctgaggaatg gagaagtcaa gagtttgcag actatggaaa gtttgtaacc attgatgaaa
    21541 tcattgatca ggttgatgtc atgatgtttc tccgtgtgca acacgaacgc catgatagtg
    21601 gagctgtatt ttcaaaagaa gactatcatg cccaacatgg cttgactcaa gaacgttacg
    21661 atcgtttgaa agaaacagca atcctcatgc acccagctcc aatcaatcgt gatgtagaaa
    21721 tagcagacca cttggttgaa gcaccaaaat cacggattgt ccaacaaatg accaatggtg
    21781 tctttgttcg aatggcaatc ttagaatccg tactagcgag tagaaacgcc aactaaaaca
    21841 caagacgtta aaagacgcca gtgagcgtcc acgagaggtg aaaatatgac aaaaagaatt
    21901 ctagtattag aagatggcac agtttttgaa ggcaaggcct tcggagcaga tattgatgta
    21961 acaggcgaaa tcgtctttaa tacagggatg accggctacc aagaatccat tacagaccag
    22021 tcttataatg gacaaatctt gacctttact tatcctttgg taggaaatta tggtattaat
    22081 cgtgatgatt atgaatccat tattccaact tgtaagggag tcgttgtttt tgaagaagcg
    22141 cgtagagcta gcaactggcg caaccaaatg accttggatg aatttttgaa agccaagaaa
    22201 attccaggga tttcaggaat tgatacgcgt gctcttacca agattatccg taagcatggt
    22261 actatgcgtg caaccttgac ccatgttggg gacagtatgg accatgtgac ggaccagctc
    22321 caagcaacag ttttaccgac agacaatatc aaacaggttt ctactaaaac gtcttatcca
    22381 gctccaggag ttggtttgag cgtggtgcta gtggactttg gtctcaagca ctcaatccta
    22441 cgtgaacttt ctaaacgcaa ctgtaacgtg acagttgttc cttattcgac aacggctgaa
    22501 gaaattctcc atctcaatcc tgatggagtt atgttgtcaa atggtccagg taacccagaa
    22561 gacgttccac aagcactcga catgattcgc ggtgtacaag gaaaaattcc aatctttggt
    22621 atttgtatgg gacaccaact ctttgcaatg gcaaacgggg ctaagaccta taagatgaag
    22681 tttggccacc gtggattcaa ccatgcggta cgtgaaatcg caacaggacg agtagacttc
    22741 accagccaga accatggtta tgcagtcagc cgcgaggatt tgccagagca cttgattatt
    22801 acccacgaag aaatcaatga caagtcagtg gaaggtgtgc gccacagata ccaacctgct
    22861 ttctctgttc aataccaccc agatgcagct cctgggccac acgacgctag ctacctattt
    22921 gacgagttta tcgagatgat ggaagttttt aaacaatcaa actaagaatg agtaaaagct
    22981 cgtcggagaa tttatgtaac tgaacatgga cggaaagctc agaatttaga gaaaccaact
    23041 tggattgtca atccttcctt gatttcctaa aattcaatcg ctttctagtc gtcctttgta
    23101 tcttatttaa tgtcgccctg agaggcgacg aatagaaagg agaagataca ttgttcgcgg
    23161 aagtgaacac gccctaagaa ctgtgtgaaa aagataaaca tcgtcttgac gctgaaggcg
    23221 tctgcgccga gtttcctatt ttcactgtgt tctttacggg ctttgtatca taaactatgc
    23281 ctaaacgtac tgatattcaa aaaattatgg tgattggttc tggtccgatt attattggtc
    23341 aggctgctga gtttgactac gctgggactc aggcttgctt gtcgttgaaa gaggaaggtt
    23401 atgaggttgt tttggttaac tcaaatcctg caaccatcat gacggacaag gaaattgctg
    23461 acaaggttta cattgaaccg attacacttg agtttgtgac acgtattctt cgtaaggaac
    23521 gtccagatgc cttgctacca acactcggtg gtcagacagg tctcaacatg gccatggaat
    23581 tgtctaaaaa tggtatctta gatgagcttg gcgttgaact tctgggtacc aaattatctg
    23641 ccattgacca agcggaggac cgtgacctct ttaaacaatt gatggaagag cttgaacaac
    23701 ccattccaga atctgaaatt gttaacacag tagaagaagc agttgccttt gcagcgacaa
    23761 ttggctaccc agtcatcgtt cgtccagcct ttaccctcgg tggtactggt ggtggtatgt
    23821 gtgccaacga gaaagaattg cgtgaaatca ctgaaaatgg tttgaaattg tcacctgtta
    23881 cccaatgttt gattgagcgt tcgattgccg gtttcaagga aattgaatac gaagtgatgc
    23941 gtgactcagc tgacaatgcc ttggttgttt gtaacatgga aaactttgac ccagttggga
    24001 ttcacacggg ggattccatt gtatttgccc ctgcgcaaac catgtcagac tatgaaaacc
    24061 aaatgcttcg tgatgcgagc ttgagcatta ttcgtgccct caagattgaa ggtggatgta
    24121 atgttcagct agctcttgat cctaacagct tcaagtatta tgttatcgaa gtaaaccctc
    24181 gtgtatcacg ttcgtcagcc cttgcttcta aagcgacagg atatccgatt gctaagttgg
    24241 ctgccaagat tgcagtaggt ttgaccttgg atgaggttat aaacccagtt acaggttcaa
    24301 cctatgccat gtttgaacca gcccttgact acgtagttgc taagattcca cgtttcccat
    24361 ttgacaagtt tgaaaagggt gaacgccgtc tcggtacaca aatgaaggcg actggagaag
    24421 tcatggcaat cggtcgtaac atcgaggaat ctcttctcaa ggcctgccgc tcccttgaaa
    24481 ttggggtgca ccacaatgaa atacctgaac ttgcagcagt ttctgatgat gccttgattg
    24541 aaaaggttgt caaagcccaa gatgaccgtc tcttctacgt atcagaagca attcgtcgtg
    24601 gctacacacc tgaagaaatt gcagagctta caaaaattga tatcttctat cttgataaac
    24661 tcttgcatat ctttgaaatt gagcaagaat tgggtgctca tccacaagat ctagaagttt
    24721 tgaaaacagc caaacttaat ggtttttcag accgtaagat tgctgaactc tggggaacga
    24781 ctgatgatca agttcgccaa cttcgcttgg aaaacaagat tgttccagtt tataagatgg
    24841 tagacacttg tgcggcagag ttcgactccg aaacgccata tttctattca acctatggtt
    24901 gggaaaatga atctatcaga tctgataaag aatctgtact tgtcctaggt tcaggtccaa
    24961 tccgtattgg tcaaggggtt gagtttgact acgcaaccgt tcactctgtt aaggctatcc
    25021 aggcagctgg ttatgaagct atcatcatga actcaaaccc agagaccgtt tcaacagact
    25081 tctctgtatc agataagctt tactttgagc cattgacatt cgaagatgtt atgaacgtca
    25141 ttgacttgga acaaccaaag ggagttatcg ttcagttcgg tggtcaaaca gccatcaacc
    25201 ttgcggagcc attggcaaaa gcaggtgtga ccatccttgg tacacaggtt gctgacctag
    25261 atcgtgccga agaccgtgac ctcttcgagc aagctctcaa agaattggat attccacagc
    25321 caccaggaca aacagctacc aatgaagaag aagcagctct tgcagctcgc aagattggct
    25381 tcccagttct tgtccgccca tcttatgtac ttggtggacg tgccatggaa atcgttgaaa
    25441 acgaagaaga ccttcgttct tacatgcgaa ctgctgttaa ggctagtcca gaccacccag
    25501 ttcttgtcga ctcttatatc gttgggcaag agtgcgaagt tgatgccatt tcagatggta
    25561 aaaatgtact cattcctggt atcatggagc atatcgaacg tgctggtgtc cactcaggtg
    25621 actcaatggc tgtttaccca ccacaaacct tgtcgcaaaa ggttcaggag accatcgcag
    25681 actacaccaa acgcttagca atcggtcttc actgccttgg aatgatgaac atccagtttg
    25741 ttatcaagga tgaaaaagtc tacgttattg aggtcaatcc acgtgccagc cgtactgttc
    25801 cattcctatc taaggtaacc aatattccta tggctcaagt agcgaccaaa ctcattcttg
    25861 gtcaaagttt atcagaactt ggctaccaaa atggacttta ccctgaaagc acccgtgttc
    25921 atatcaaggc acctgtcttc tcctttacca aactagctaa ggtagacagc ttgctcggtc
    25981 ctgaaatgaa gtcaacaggt gaagttatgg gttctgatgc tactttggaa aaagctctct
    26041 ataaagcctt tgaagcttct tatctacact tgccaacttt tggtaatgtc gtctttacca
    26101 tcgctgatga tgccaaagaa gaagccttga acttggctcg tcgtttccaa aacattggtt
    26161 atggaatcct tgcgacagaa gggacagcag ccttctttgc tagccatggc ttgcaagccc
    26221 aacctgttgg taagattggt gatgacgata aggatatccc aagttttgta cgcaaaggaa
    26281 gaattcaagc tatcattaac acagtaggaa caaaacgaac tgctgacgaa gatggtgagc
    26341 aaattcgtcg ttcagccatt gaacacgggg tgcccctctt cacagcgcta gatacagcta
    26401 atgccatgct taaggtactt gaaagtcgta gctttgttac agaagcgatt taaatagaaa
    26461 aataagatat agcagactga atttagaaca gtacacagga atactaaaat atttctagaa
    26521 attaatttga attttctaat tgatttgttc gcatcttatt tcaatctact atagaaaaaa
    26581 gtctttaaaa ttataaaacg catcatatca aggtttttca aaaaccttga tatgatgcgt
    26641 tttattgtgg gaatatgtat ttcattttct actaaaatta tgtttttgaa taacctctat
    26701 cttagtagtt tgtataatcc ccctcaatca gtttttgcga taagctttaa tgctatgact
    26761 ataccactct tgcatttctt ttgatggtgg tgtcatataa tcgccataca tctgggttaa
    26821 aaattggtca tattttttgg gaacaggcaa catacggccc tcaaactcag ttaaaatcag
    26881 ttctttaaag gtatcaactg ggaagatttc tttcatccct tccttaccga tcccaactcc
    26941 tccttcatat tgaggaatgt tggttacagc atttttgact agttgatcaa ttttcttgta
    27001 aaagtagcga ggattgacaa atcggagagc gtaccagcta cataatctaa gaaaatcttt
    27061 tagtttgcta tcaccgtgaa ctgctcgtga ttttttgatg taagctagtt gacgaagggc
    27121 cacatactta tagctcttgt cgacaatgct caagtctgta aattgatcaa ttgggaagac
    27181 atcgatgaaa aggctggtat catgacgctt gtacttaaca tggtcttcta taacagtaga
    27241 agtatccaaa atcgatgcga aattatggaa gtaccaagaa gatgtatcgt aggaaagaac
    27301 cttgtagcga gggtgatttt cttcttcaat aatcttcagt aaacgctcat aatcctcacg
    27361 ataaagggaa atatcaatat catcatccca aggaatcata cctttgtggc ggatggctcc
    27421 aagcatggtt ccataactga gaaaataagg aatatcatgt ttcttacaag tctcatcaat
    27481 atagtccagc agggctagtt gaatttcttt aatttctttt ttttctaaat attgcatcct
    27541 aatcctccaa tttataagcg tgaaattcat gactgtagaa gcgtttttct tctggtggta
    27601 gggtcatata atctccataa aattgtgtca aaatagtatc aaatttttca ggtgcaggaa
    27661 ggcttaaatt ctcaaagggt aaatcgattg ttttatcaaa ggtagcactt gggaagactt
    27721 ccttttcctt aaattttgaa ggaataaaag ccatatactg cccattttca cgactatatt
    27781 tttgaatttc tttctcgatt ttatttgcaa aataacgagg agaaaccagt cgaaggagca
    27841 accaaaaggc tgttcgtatc caatctttta aaaggctatc cttatagaca atatttttat
    27901 gtttactgaa agacagcagt ttgaagcttt ctagtttata acaagtatca atgaccttag
    27961 gatcatcaaa gcgatccata gggaaaatat cgataaagat accagactca taggtttttg
    28021 tatttcgagt atcaataatt ttagtcgtac tgtcggttat cttgataaag ttgttaaagt
    28081 agttcttatc agtttctaag gataggagct tatacttgct tttttccttt tgaaaaatgt
    28141 taataaatcg ttggtagtct tctctaggca tggataaatc aatatcgtcg tcccaaggga
    28201 taaagccctc atgtcgaacc gccccaatca gagtaccgta gttaataata tagttgatat
    28261 tgtgcttttt acagagagta tcaatataat tcaaaatttc taattcaatt tgtttggcat
    28321 cttcaacggt tagttgtttc attttaaact cctaagattt tttaaattta ttttttaagg
    28381 ctaggacatg gtttaaaaat tcatagaaaa tgctatcttt tgtgaagaca agtagactaa
    28441 tataagagat agctgatagc aagacaatca aaccagtatt aatcaaaaat ggcaaattaa
    28501 tgaccatatc cacaggatac acgaaattaa tcaggaaata aattgctaca aaggaaagtg
    28561 aaaagagaga gtatcgaaca gtatagctaa agatatgtcc caagtggatg agttgttttc
    28621 tatggatgaa aatgatatag aaaacaagta gagaagcctc tgataacata gttgtcagta
    28681 agtagtattc gggagccacg atatggttga aaaagaggag actattcaag cccaaattga
    28741 gtagcccagc aaagactgta tagactgtga tacgtttttc atagccattg gtaaagagaa
    28801 tttgggaacc aagaatggta tctaaggcca ggataatcgt acgaaaagcg aagagagagg
    28861 tcaagatgtc gcctccgata tatttttcac taccgtaaag taggatggca tttggtccta
    28921 aaaccatgag tccaaaactc agtggaatga taaagaagtt aaagattcga ctacctctat
    28981 taaccagaga aacataggct tctttgtctc ctttccccag atagtaactg agacgaggca
    29041 cactcactcc aattgcacct gttacaaccc cagctataac ggtcacaatt cgctgagcta
    29101 tggtatagta actaacgttg acatcaatcc ctgttttaac gaggaagagg cgatctaaaa
    29161 aagtgaagag catattggca ttggcaaaga ctaacatggc tgtcagaggg agaaagagtg
    29221 gtttaaaatc acttaggtga atcttaacaa gtttgatgtc tcttttaatc caaaaataac
    29281 taatcaggta gttaatcagc gtcgataaac tcatcacaag tgtatagaca acaatatcgt
    29341 gttcattttt aacaaataag aaaatagaga ccagcatcag gatacggatg aaggcagttt
    29401 tgtaaaagag aaaactgtaa ttttccagag cttcattgac ccattcgatt gaaaaaatct
    29461 gggcaatgag ttgaatcccc ataacaaggt agaccttttt gacgattgga ttatcagtaa
    29521 agaagagagg ataggctagg atatagacag cagtggtcaa aatcgtacaa gcgatgcaca
    29581 aataaaaaag actagaaaag gttctgttaa gatctttttt gttatccttg acattactga
    29641 tagcccttaa accgtagtta tagacaccat aagttgcaaa gggcaagaaa aatgacaaaa
    29701 tagtgtcgac tgagttgaag taaccatagt cagttcggtc caagacacgc gcgacatagg
    29761 ttccagttag gatgggaaaa ataatattca agacacgaat tcccatgtaa gatagagcat
    29821 ttaattttat acttttcatt caatttacct cgtttttcat tatatcataa agttagctaa
    29881 taagaaatga agggcagtaa gtcaagtaat cactttgaag tttcaaatct taagttttaa
    29941 gttttcttta aggaaagtat attattctga aggactctaa aatttcgcag ccatttatta
    30001 gtaattgcta cagaattcct agtcattact agaaatggac tagtttcttt gaataataga
    30061 actgcataat tctcctattc tagaagggga ggaccagtat ttcttttatg ataggactag
    30121 attgtggtat aatagagaga ataagttttt ttagtaagac aaaggagaaa atagatgatt
    30181 tatgcaggaa ttcttgccgg tggaactggc acacgcatgg ggatcagtaa cttgccaaaa
    30241 caatttttag agctaggtga tcgacctatt ttgattcata caattgaaaa atttgtcttg
    30301 gagccaagta ttgaaaaaat tgtagttggt gttcatggag actgggtttc tcatgcagaa
    30361 gatcttgtag ataaatatct tcctctttat aaggaacgta tcatcattac aaagggtggt
    30421 gctgaccgca atacaagtat taagaaaatc attgaagcca ttgatgctta tcgtccgctt
    30481 actccagagg atatcgttgt tacccacgat tctgttcgtc catttattac acttcgcatg
    30541 attcaggaca gtatccaact tgcccaaaat catgacgcag tggacacagt ggtagaagcg
    30601 gttgatacta tcgttgaaag taccaatggt caatttatta cagatattcc aaatcgtgct
    30661 cacctttatc aaggacaaac acctcaaaca ttccgttgca aggacttcat ggacctttat
    30721 ggatctcttt ctgatgaaga gaaggaaatc ttgacagatg catgtaaaat ctttgtgatc
    30781 aaaggaaaag atgtggcctt ggccaaaggt gaatactcaa atctgaagat tacaaccgta
    30841 acagatttga agattgcaaa aagtatgatt gagaaagact agtaaaatga ttaatcaaat
    30901 ttatcaacta actaagccta agtttatcaa tgtcaaatat caggaagagg ctattgacca
    30961 agagaatcat atccttatcc gtcccaacta catggctgtc tgtcatgcgg atcagcgtta
    31021 ctatcaggga aaacgtgatc ccaagatttt gaataaaaag cttccaatgg caatgattca
    31081 cgagtcatgt ggaaccgtca tttctgaccc gaccggaacc tacgaggttg gtcaaaaagt
    31141 tgtcatgatt cccaatcagt ctcctatgca gagtgatgaa gaattctatg aaaactacat
    31201 gacagggacc catttcttgt ctagtggatt tgatggcttt atgagagagt ttgtttctct
    31261 ccctaaagat cgtgtggtgg cttatgatgc tattgaagat acggttgcag ccattacaga
    31321 gtttgtcagt gtgggcatgc acgctatgaa tcgtctattg actcttgctc atagcaagcg
    31381 ggagcggatc gccgttattg gagatggaag tttagctttt gtggttgcca atattatcaa
    31441 ctatactttg ccagaagcag agattgtggt tattggtcgt cattgggaaa agttggaact
    31501 cttctcattt gccaaagaat gctatattac ggataatatt cctgaagagt tggcctttga
    31561 ccatgctttt gaatgttgtg gtggtgatgg tactggacca gctattaatg acttgattcg
    31621 ctacattcgt cctcagggaa cgattctcat gatgggagtt agcgaatata aagtcaatct
    31681 caatactcgc gatgccttag aaaagggctt gattttggtt gggtcatctc gttctggtcg
    31741 cattgatttt aaaaatgcta tccaaatgat ggaagtcaag aaatttgcca atcgtcttaa
    31801 aaatatcctt tatctagaag aacctgtaag agaaattaaa gatattcatc gtgtctttgc
    31861 aaccgattta aacacagcct ttaaaacagt gtttaagtgg gaagtataag tactggaggt
    31921 taattgtgga gaaaatcatt aaagaaaaaa tttcttcctt acttagtcaa gaagaggaag
    31981 tcctcagtgt tgaacaactg ggtggaatga ccaatcaaaa ctatttggcc aaaacaacaa
    32041 ataagcaata cattgttaaa ttctttggta aagggacaga aaagcttatc aatcgacaag
    32101 atgaaaagta caatcttgaa ctactaaagg atttaggctt agatgtaaaa aattatcttt
    32161 ttgatattga agctggtatc aaagtaaatg agtatatcga atctgcgatt acgcttgatt
    32221 caacgtcaat caagaccaag ttcgacaaaa ttgctccaat attacaaact attcatacgt
    32281 ctgctaagga attaagagga gaatttgctc cttttgaaga aatcaaaaaa tacgaatcct
    32341 tgattgaaga acaaattcct tatgccaact atgaatctgt tagaaatgca gtcttctcct
    32401 tagagaaaag actggctgac ttaggtgttg acagaaaatc ttgtcatatc gatttggtgc
    32461 ctgaaaactt tatcgaatca cctcaaggac gactttattt gattgactgg gaatattcat
    32521 caatgaatga tccaatgtgg gatttggctg ccctcttttt agagtctgaa ttcacttccc
    32581 aagaggaaga aactttctta tctcactatg agagtgacca aacaccggtt tctcatgaaa
    32641 agattgctat ttataaaatt ttacaagata ctatttggag tctatggact gtctataagg
    32701 aagagcaagg tgaagatttt ggtgactatg gtgtgaatcg ttaccaaaga gctgttaaag
    32761 gtttggcttc ttatggaggt tcagatgaaa agtaaaaacg gagttccttt tggccttctc
    32821 tcaggtattt tctggggctt gggtctaacg gttagtgctt atatcttttc gatttttaca
    32881 gatttgtcac cctttgtggt ggctgcagct catgattttt tgagcatctt tatcttacta
    32941 gcttttctct tggtaaaaga agggaaagtt cgcctctcaa ttttcttaaa tattcgcaat
    33001 gtcagtgtta tcatcggagc cttgctagca ggccctatcg gtatgcaggc caatctttat
    33061 gcagttaagt atatcggaag ttctttagct tcatctgtat cggctattta ccctgcgatt
    33121 tcagttctat tggctttctt ctttttgaag cacaagattt cgaaaaatac tgtatttggg
    33181 attgtcttga ttattggagg gattattgcc cagacctata aagttgaaca ggttaattct
    33241 ttctacattg ggattctttg tgctttggtt tgtgctattg catggggaag tgagagtgtt
    33301 cttagctctt ttgccatgga aagtgaattg agtgaaatcg aagccctctt aatccgtcaa
    33361 gtaacttcgt tcttgtccta tcttgtgatt gtgctcttct ctcatcagtc atttactgca
    33421 gtagccaatg gacaattgct aggtctcatg attgtttttg cagcctttga tatgatttcc
    33481 tacttggctt attatatcgc tatcaatcgc ttgcaaccag ccaaggctac aggcttgaac
    33541 gtgagctatg tagtatggac ggtcttgttt gcagttgttt tcttgggtgc accgctagat
    33601 atgctgacca ttatgacgtc acttgtcgtc attgctggag tttatattat tattaaagaa
    33661 taaaggagat tcgtgtgaaa gccatcatct tagcagcggg attgggaact cgcttgcgtc
    33721 ctatgactga aaatacccct aaagccttgg ttcaggttaa tcaaaaacct ttgattgagt
    33781 accaaattga gtttctcaaa gaaaaaggaa tcaatgacat catcatcatt gttggttatc
    33841 ttaaagaaca attcgattac ttgaaagaga aatacggtgt tcgtctcgtt ttcaatgata
    33901 aatacgctga ctacaataac ttttactctc tctatcttgt aaaagaagaa ttagccaata
    33961 gttatgttat cgatgccgat aactatctct ttaaaaatat gttccgcaat gatttgacac
    34021 gttctactta ttttagtgtc tatcgtgaag attgtaccaa cgaatggttc ttggtctatg
    34081 gagatgacta caaggttcaa aacattattg ttgatagcaa ggcaggtcgc atccttagtg
    34141 gtgtatcctt ctgggatgct ccaacagcag aaaaaattgt tagctttatc gataaggctt
    34201 atgcaagcgg cgaatttgtt gatctctatt gggacaatat ggttaaggat aacatcaaag
    34261 agctagatgt ctatgttgaa gaattagaag gcaatagcat ttatgagatt gatagtgtcc
    34321 aagactatca taaattagaa gaaattctta aaaacgaaaa ttaaaagctt ccaacatctg
    34381 acaaaatagt cggatgtttt tttgattttt tacgaacttt tacgaataga tagatgagta
    34441 gaaaaagaaa tggagttatt tatgaaaatc acaaactatg aaatctataa gttaaaaaaa
    34501 tcaggtttga ccaatcaaca gattttgaaa gttctagaat acggtgaaaa tgttgatcag
    34561 gagcttttgt tgggtgatat tgcagatata tcaggttgcc gtaatccagc cgtttttatg
    34621 gaacgttatt ttcagataga cgatgcgcat ttgtcgaaag agtttcaaaa atttccatct
    34681 ttctctattt tagatgactg ttatccttgg gatttgagtg aaatatatga tgcgcctgta
    34741 cttttatttt acaagggaaa tcttgacctc ctgaaattcc cgaaggtagc ggtcgtgggc
    34801 agtcgtgctt gtagcaaaca gggagctaaa tcagttgaaa aagtcattca aggcttggaa
    34861 aatgaactgg ttattgtcag tggtctggcc aagggcattg acacagcagc tcatatggca
    34921 gctcttcaga atggcggaaa aaccattgca gtgattggaa caggactgga tgtgttttat
    34981 cctaaagcca ataaacgctt gcaagactac atcggcaatg accatctggt tctaagtgaa
    35041 tatggacctg gcgaacaacc tctgaaattt cattttcctg cccgtaatcg catcattgct
    35101 ggactttgtc gtggtgtgat tgtagcagag gctaagatgc gttcaggtag tctcattacg
    35161 tgtgagcgag caatggaaga aggacgcgat gtctttgcta ttcctggtag cattttagat
    35221 ggactatcag acggttgcca tcatttgatt caagaaggag caaaattggt caccagtggg
    35281 caagatgttc ttgcggaatt tgaattttaa aaatgaccta agctagaatt ctaagaaaaa
    35341 atcaatttta agagaaaatg aacccaacat ttccataata aaacgcatat tagcaagttt
    35401 ttaacacttg ataatatgcg ttttttctaa gtggattagt agagtagagg atttttctca
    35461 tataatactc ttcgaaaatc tcttcaaact acgtcagctt ccatctgcaa cctcaaaaca
    35521 gtattttgag ctgacttcgt cagtcttatc tacaacctca aagcagtgct ttgagcaacc
    35581 tgtggctagc ttcctagttt gcgctttgat tttcattgag tataagggaa agtatagtga
    35641 attgaaataa gatgtgaaca actctatcag gaaagtcaaa ttaatttata gaaatatttt
    35701 agcagccaag gtgcactgtt atagattcaa tacactataa tttagtgtaa ttgagaaagg
    35761 agaaatgatt gtgattgatg ttggctaggt tatgttcaat gattcctacc gtctcaaatc
    35821 ttgtcagtaa ggaaaaataa attcttcaaa agtagagatt acaaggcttg tttaagaaag
    35881 aattcaaaga ccttgacaaa taaaaataaa atggttatta taaaaaatgg tctgaaatag
    35941 atgatgatac ttttcgaaaa tctttttacg tcagctcagc tttgccttgc tgtgttttga
    36001 gcaagctacg gttagcttcc gagtttgatt ttcatttact agaaatgaaa ctgatgagag
    36061 atatcagtag acatttgagt caggatatta tggaaaatga taaaaagagc tcgtgagatt
    36121 ggcatatcag actactaaag tattgagttt gttaggattt tagcgactag ttagctggga
    36181 aaggaagata tttgtgacaa ataataaact gtattcgttg atagaattta gaaataaaat
    36241 atatgaagaa ttagaacttt ccagaagtga tttagcgatt ttactatgtg ccatgcttat
    36301 cgcctctatc ggattaaata tggattcgac tcccgtgatt attggagcca tgttaatctc
    36361 tcctttgatg acacctattc tgggagtggg gctctctcta gctatatttg attttaaatt
    36421 gttaagaaaa tcttttaaaa tattagctat tcaaattctt gccagtttaa tagcttcaac
    36481 actttatttt tatctttctc ccatttcgta tgctagttcg gaaattgttg ctagaacctc
    36541 tccgactatt tgggatgttc tcattgcttt tgtaggaggg atagcaggta ttattggtgc
    36601 taggaaaaaa gagaccaata atattgttcc tggtgttgct attgcaaccg ccttgatgcc
    36661 tcctctttgt acagtaggtt atgctattgc ttctgctaat ctaaaattta tcataggctc
    36721 ctcttaccta ttcctcatca attgtagctt tattgtcatt gcgacttata taggtgttag
    36781 gttgatgatg gttaagaaac attattttaa agataatgaa gaagactcta aaatgcgtag
    36841 gattttgctt ctagttgctg ttttgctgat gattccgagt ttcatctctg caacgacttt
    36901 agtgagagaa acgttgaaaa aagagtccct taagaaattt atatcagagc agtttcaggg
    36961 gcataatatt ttgaaaaaaa cctattctaa aaagactcat accctaaagc taaccatttc
    37021 aggaaattat ttgacagaag aagaactcga tatgatttca agtaagagag gtgactatgg
    37081 tttaagtgat gtttctgttc aagtttcaca attgtctgat tcagaacaac ttagtaagga
    37141 agaactggtg gagtatttct tccagtatat caaggataag gaagcaaaag aaaaggaaaa
    37201 agctaataag ttttatacag agtctgagga gcaataattt cttgagaata gctggtttct
    37261 ctcgtgagtc ttctatgtat atcaaaggaa gactgaggtt ttaagtataa aacttttctt
    37321 ctattatagt agctaagtct tgacagggtt caaaaaatgg tttacacttt ataaagttta
    37381 ttactttgaa aaggtgtgat actgtggcta cggcaacaaa aaagaaaaaa tcaacagtta
    37441 aaaaaaatct agtcatcgtg gagtcgcctg ctaaggccaa gacgattgaa aaatatctag
    37501 gcagaaacta caaggtttta gccagtgtcg ggcatatccg tgatttgaag aaatccagta
    37561 tgtccgtcga tattgaaaat aattatgaac cgcaatatat caatatccga ggaaaaggcc
    37621 ctcttatcaa tgacttgaaa aaagaagcta aaaaagctaa taaagttttt ctcgcgagtg
    37681 acccggaccg tgaaggagaa gcgatttctt ggcatttggc ccatattctc aacttggatg
    37741 aaaatgatgc caaccgtgtg gtcttcaatg aaatcaccaa ggatgcagtc aaaaatgctt
    37801 ttaaagaacc tcgtaagatc gatatggact tggtcgatgc ccaacaagct cgtcggatct
    37861 tggatcgctt ggtagggtat tcgatttcgc ctattttgtg gaagaaggtc aagaagggct
    37921 tgtcagcagg tcgcgttcag tccattgccc ttaaactcat cattgaccgt gaaaatgaaa
    37981 tcaatgcctt ccagccagaa gaatactgga cagttgatgc tgtctttaaa aagggaacca
    38041 aacaatttca tgcttccttc tatggagtag atggtaaaaa gatgaaactg accagcaatg
    38101 acgaagtcaa ggaagtcttg tctcgtctga ctagtaaaga cttttcagta gatcaggtgg
    38161 ataagaaaga gcgcaagcgc aatgctcctt taccctatac cacttcatct atgcagatgg
    38221 atgctgccaa taaaatcaat ttccgtactc gaaaaaccat gatggttgcc caacagctct
    38281 atgaaggaat taatattggt tctggtgttc aaggtttgat tacctatatg cgtaccgatt
    38341 cgactcgtat cagtcctgta gcgcaaaatg aggcggcaag cttcattacg gatcgttttg
    38401 gtagcaagta ttctaagcac ggtagcaagg tcaaaaacgc atcaggtgct caggatgccc
    38461 atgaggctat tcgtccgtca agtgtcttta atacaccaga aagcatcgct aagtatctgg
    38521 acaaggatca gctcaagcta tataccctta tctggaatcg ttttgtggct agccagatga
    38581 cagcggccgt ttttgatacc atggctgtta aattgtctca aaatggagtt caatttgctg
    38641 ccaatggtag tcaggttaag tttgatggtt atcttgccat ttataatgat tctgacaaga
    38701 ataagatgtt accggacatg gttgttggag atgtggtcaa acaggtcaat agcaaaccag
    38761 agcaacattt cacccaaccg cctgcccgtt attctgaagc aacactgatt aaaaccttag
    38821 aggaaaatgg ggttggacgt ccatcaacct atgcgccaac cattgaaacc attcagaaac
    38881 gttattatgt tcgcctggca gccaaacgtt ttgaaccgac agagttggga gaaattgtca
    38941 ataagctcat cgttgaatat ttcccagata tcgtaaacgt gaccttcaca gctgaaatgg
    39001 aaggtaaact ggatgatgtc gaagttggaa aagagcagtg gcgacgggtc attgatgctt
    39061 tttacaaacc attctctaaa gaagttgcca aggctgaaga agaaatggaa aaaatccaga
    39121 ttaaggatga accagctgga tttgactgtg aagtgtgtgg cagtccaatg gtcattaaac
    39181 ttggtcgttt tggtaaattc tacgcttgta gcaatttccc agattgccgt catacccaag
    39241 caatcgtgaa agagattggt gttgagtgtc caagctgtca tcagggacaa attattgagc
    39301 gaaaaaccaa gcgtaatcgc ctattctatg gttgcaatcg ctatccagaa tgtgaattta
    39361 cctcttggga caagcctgtt ggtcgtgact gtccaaaatg tggcaacttc ctcatggaga
    39421 aaaaagtccg tggtggtggc aagcaggttg tttgtagcaa aggcgactac gaggaagaaa
    39481 agattaaata agaggagggg cccgaaagta aatttcaggc tccttttggt tagaacttga
    39541 caaaatacat cattttatgc gaaactagaa gaagattatt ttatactcaa tgagaatcaa
    39601 agagcaaatt agaaagctag tcgcaggttg ctcaaagcac tactttgagg ttgaagatgg
    39661 aagctgacct ggtttgaaga gattttcgaa gagtattaac taggagaagt tatgcgtatt
    39721 atctatctaa ttattggttt tttatcgctg accttggcta ttgttggggt tgttttaccc
    39781 ttgttgccta caacaccttt ccttttgttg tctattgctt gtttctccag aagttccaag
    39841 cgattcgaag attggcttta tcataccaag ctctatcaag catatgtagc tgattttcgt
    39901 gagaccaagt ctattgcgcg tgaacgaaag aaaaaaatca tcgtctctat ctacgtcttg
    39961 atgggaattt ctatttattt tgcacctctt ttaccagtca aaatcggtct gggtgctttg
    40021 accatcttta ttacttatta tctcttcaag gtcattccag acaaagaata gttaaaacag
    40081 tagttatttg ccttgataaa attgaaagca tattcataac aatatgatat aataaaattg
    40141 aagtaatatt caaggagaat caaatgattt acgaattttg tgctgaaaat gtgactttac
    40201 ttgaaaaagc gatgcaggct ggagctcgtc ggattgaact ctgtgataat ctagcagttg
    40261 gtgggacaac acccagctat ggagtgacta aggcagcggt tgaactggca gctaactacg
    40321 atacaaccat catgaccatg attcggccac gtggtggtga ctttgtctat aatgacctag
    40381 aaattgctat catgctagaa gacattcgtt tgactgctca ggctggaagt caaggggttg
    40441 tatttggagc tttaactgct gataaaaagt tggataagcc taatctggaa aagttaattg
    40501 ctgcatcaaa aggaatggaa attgtctttc acatggcctt tgatgaacta agtgatgaag
    40561 atcaaccgga agctattgac tggctcagtc aagccggtgt cactcgtatc ctaactcgtg
    40621 ctggtgtgtc tggcgactcc ttagaaaaac gttttgttca ctatcacaga attttggagt
    40681 acgctaaagg taaaattgaa attctaccag gtggggggat tgaccttgaa aaccgtcaaa
    40741 cctttatcga ccaggtgggg gtaacacaat tgcatggtac taaggttgtt ttttaaaaaa
    40801 tagaaaggaa ctgctagctt tgggtagcag ttttcactta tgtttgaaat ttttaaatcc
    40861 tatcaattta atcaagaaaa ggctcatgat tatggtttta tagaaaatag cgaagtctgg
    40921 acatatagtt gccagatttt gcaaggtgac tttgtcatga ctgtgtccat cactgctgat
    40981 aatgtgaact ttcaagtctt tgaccaagag actggtgacc tctatcctca cgtttatatg
    41041 gaaagcatga ggggaagttt tgtcggaaat gtccgtgagg cttgtctgga gattctttac
    41101 cagattcgga aggcttgttt tgatgtgcaa gattttatct gtcatcagac taagcgtatc
    41161 atgactcaag ttcaggaaaa gtatggaaac cagttggagt atctgtggga aaaatcgcct
    41221 gatacagctg tattgcgcca tgaaggcaat caaaagtggt atgccgtctt gatgaaaatc
    41281 tcttggaata agctggaaaa gggcagagaa ggacaagtgg aagcagtcaa cctcaagcat
    41341 gaccaagtag ctaatttgct ttcacaaaag gggatttatc cagccttcca tatgagcaag
    41401 cgctactgga ttagtgtgtc ccttgatgat actttatcag atgaagaagt actggaattg
    41461 atagaaaaaa gttggaactt aacctctaaa aaatgaaata ttttaataat tttcatgaac
    41521 tttcaattag ctaaatattc tttactgaag agatttttag aaaatatagg atttaccaca
    41581 ctagaggaat atggtgccat cttcaaatac ctgattgaga atgtcaagac ggatcgtcag
    41641 atcatctatt cgcctcactg tcatgatgac ctcggaatgg cagtggcaaa tagccttgct
    41701 gctgtcaaga atggtgcagg acgtgttgaa gggactatca atggtattag ggagcgagct
    41761 gaaaatgctg ctttggaaga aattgcagtg gctctcaata ttcgccaaga ttactaccaa
    41821 gtagaaacca gtattgtcct aaatgagacc atcaatacgt cagaaatggt ttctcgcttc
    41881 tctggtattc cagttcctaa aaacaaagcc gtcgttggtg gcaatacctt ctcccacgaa
    41941 tctggtattc accaagatgg agtccttaaa aatcctctca cttatgagat catcacacct
    42001 gaattggttg gtgttaagat tctgcttgga aaattatctg gtcgccatgc ttttgttgag
    42061 aaactgagag aattggccct agattttaca gaagaggata tcaaaccact ctttgctaag
    42121 ttcaaggcac tggtcgataa gaagcaagaa atcacagatg cagatattcg agctttggta
    42181 gctggaacca tggttgaaaa tccagaaggc ttccactttg atgatttaca acttcaaact
    42241 catgcagata atgacattga agcgctcgtt agcctagcca atatggatgg tgagaaagtc
    42301 gaatttaatg cgacagggca aggttccgtt gaagcaatct ttaatgctat cgataagttc
    42361 tttaaccaat ctgttcgttt ggtgtcctac actatcgatg cggtaacaga tggaatcgat
    42421 acccaggatc gggttttggt cactgttgaa aacagagata cagaaaccat ctttaatgca
    42481 gcagggcttg attttgatgt gttgaaggct tctgctattg tctatataaa cgctaatacc
    42541 tttgttcaaa aagagaatgc aggtgagatg ggacgcagtg tttcttacca cgatatgcct
    42601 agtgtgtaaa ggagaaggct atggcaaaga aaatagtagc tctagcagga gacggaattg
    42661 gcccagaaat catggaggtt ggtttagaag ttctggaggc tctagctgaa aaaacaggtt
    42721 ttgactatga gattgacaga cgaccgttcg gaggtgcaga tattgatgca gcatgacctc
    42781 ccttacctga tgaaaccctt aaggcaagta gggaagcaga tgctatccta ctagtagcta
    42841 tcggtagtcc tcagtatgat ggagcagtgg ttcgccctga acaaggcctg atggctctcc
    42901 gtaaggaact caatctttac gctaatattc gtcctgtaaa aatctttgac agtctcaagc
    42961 atttgtcact actcaaactg gaacgaattg ctggtgtaga ctttgtcgtg gtgcgtgaat
    43021 tgacaggcgg gatttacttt ggagatcata ttcttgaaga gcgcaatgcg cgtgatatca
    43081 acgactatag ctatgaggaa gtggagcgga ttattcgcaa agcctttgaa attgcaagaa
    43141 atcgcagaaa aatcgttact agtatcgata agcaaaatgt tctagcgacc tcaaaactct
    43201 ggcggaaagt agctgaggaa gtcgcacagg atttcccaga tgtaaccttg gaacatcagc
    43261 tggtagactc agctgctatg cttatgatta ccaatcctgc taagtttgat gttattgtaa
    43321 cggagaatct ttttggagat attttatctg atgaatcaag cgtcttatct ggtacacttg
    43381 gggttatgcc atcagccagt cattctgaaa atggaccaag tctctatgaa cctattcaca
    43441 gttcagcacc tgatattgca ggtcaaggaa ttgccaatcc tatttccatg attttatcag
    43501 tttccatgat gttgagagat agtttcggac gttatgagga tgcagagcgt atcaaacgtg
    43561 ctgttgagac aagtctggcg gcaggaattt taacgagaga tataggaggt caggcttcaa
    43621 caaaggaaat gacggaagct attattgcaa ggttatgaag ttagacgaaa aaattactct
    43681 agtccttttg atttggaatg tcatcatttt cttgatttat ggtattgaca aatctaaggc
    43741 aaggagaaga gtttggcgca tccctgagaa aatcttactt attttagcct ttacttttgg
    43801 tggttttggt gcctggctag caggaatcat ctttcaccac aagactcgaa aatggtactt
    43861 taaaatagtt tggtttcttg ggatggtgac cacactagta gccttatatt ttatttggag
    43921 gtaatggatg gcagggtctt cgagggaata cgctgcttgg gctctagcgg actatggttt
    43981 taaggtcgtg attgcaggat ctttcggtga cattcattac aataatgaac tcaataatgg
    44041 catgttgcca atcgttcagc ctagagaggt tagagagaaa ctagcccagc taaaaccaac
    44101 cgaccaggta actgtggact tggaacaaca aaaaatcatc tcaccagttg aagaattcac
    44161 cttcgagata gatagcgagt ggaaacataa actcctaaat agtttggatg atatcggtat
    44221 taccttgcag tatgaagagt tgattgctgc ttatgaaaaa caacgaccag cctactggca
    44281 ggattagaaa aaatagaaaa ggagatatag taaactgaaa taagatgtaa acaaatgaat
    44341 tggagcttaa catccatttc cagcaatttt ttagaaacta cagtggacta ttctggattc
    44401 aacacattat aaaattatga caaaacacat tcacaagaag gctacgacat tttaaaaggt
    44461 gagggcggat gtatcgtttg ccctactaaa gttggttaca ttatcatgac cagtgacaag
    44521 gcaggacttg agcgtaagtt cgcagccaaa gaacgtaagc gtaacaaacc aggtgttgtt
    44581 ctctgcggta gcatggatga actttgcgct ttagcgcaac tcaacccaga aattgaagca
    44641 ttctactaaa aacattggga tgaagatatt cttcttggtt gtatccttcc ttggaaacca
    44701 gaagcctttg aaaaactcaa agcatacggg gatggccgtg aagaacttat gactgatgta
    44761 cgtggtacta gctgttttgt tatcaagttt ggaaaagcag gtgaacaatt ggctgccaag
    44821 ctttgggaag aaggtaaaat ggtctacgcc tcatctgctt caatgacaaa acgattgaaa
    44881 ctcgctatga gcaaggtgta atggtgtcta tggtcgataa ggacggcaaa ctcatcccag
    44941 aacaaggagg agcacgttca acttcaccag ctccagttgt gatccgtaaa gggcttgaca
    45001 ttgatatagt aaaatgaagt aagagcagga caaattaatc aggacagtca aatcgatttc
    45061 taacaatgtt ttagaagtag aggtgtacta ttctagtttc aatccactat aaaatcatga
    45121 tgcacctgtc agatactttt aactcatggg actaccgtca ggttgagtat tattaggata
    45181 gagaagaagt ctagtgttaa ggacattaca gctcataata ctaggctttt ttatagaaat
    45241 tcttttcttt ttctatagga tatggtattc tatgtagaaa atatatgtta ataagtaatg
    45301 ccaatattta aacatcatta gtaaaaggag ttagattgat gaataaaaga aaagttagtt
    45361 tagaagattt ttataaatgg tatagtctaa ataaagaaga gttattaaat aaggcaactg
    45421 ttggtgaaaa gtttaatgat aaattaaaag aagagtttct ccaggaatgg cctttggata
    45481 ggattttaac aatgtcaatc gatgaatatg taataggaaa gggacagcaa aataagtctt
    45541 tatgctacgc tcttgagaag ggaaaataca aaaatctatt tcttggaatt tctggtggct
    45601 cagcttcaaa atttggtatt tattggaata aaaaaacaaa caaatataaa gatcaagcta
    45661 ataatgagat ttcagagttg gatcagcgat tttcaaaatt aaaatcagat ttgtatgaaa
    45721 ttatcaaaga aggtattcgt tttaactttg aaaatcctat ttttgatatg aaaagatcaa
    45781 caaatgaatt tattggtcgt tctgctatgg tgacaaaatt actttgtatc tatactgagg
    45841 gagatccttt ctttggtgta aatattaata gtcagaaaga attttggaac cactttgttt
    45901 ctcagacaaa tcaaggtgga ccttatctgc aaaatcataa aataattgaa ctgatgtcca
    45961 aaacttatcc tgagttggag ccatcgaaat taggaactat gctttttgag tattctaagc
    46021 tttttatgga aaataaggaa gacaatagta caatggattc atcaaacaat tttcgtcatc
    46081 aattaactca atctctatta aagtctccaa accttatcct tcgtggtgct cctggcacag
    46141 gaaagactta tcttgctaaa gaaattgcca aagaattaac ggatggcaac gaagatcaaa
    46201 tcggatttgt acaatttcac ccatcatatg attatacgga ttttgtagaa ggtttaagac
    46261 cagtatcaaa tggggatgga gctattgagt ttaggctaca ggacggtatt tttaaagatt
    46321 tttgtcagaa agcaaaagaa acccaattga ttggaggaca agataatttt gatgaggctt
    46381 gggattctta cttagaatat ataaatgttg ctgaagaaaa agaatatata acaaaaacat
    46441 cttacttatc tgttaatagt agacaaaatt tgtcagtaaa ttatgatagt ggtgttccag
    46501 gatggtcact acctag
//
LOCUS       DAVBCB010000009        45835 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVBCB010000009 DAVBCB010000000
VERSION     DAVBCB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45835
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.009"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1122
                     /locus_tag="VQU28_000490"
     CDS             <1..1122
                     /locus_tag="VQU28_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2475903.1"
                     /translation="STSASASASTSASESASTSASTSASASASTSASASASTSASTST
                     STSASTSASTSASTSASTSASTSASESASTSASTSASTSASASASISASESASTSASA
                     SASTSASASASTSASESASTSASASASTSTSASESASTSASASASTSASASASTSASA
                     SASTSASESASTSTSASESASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASESASTSASESASTSASASASTSVSNSANHSNSQVGNASGSTGKSQ
                     KELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
     gene            1183..3268
                     /locus_tag="VQU28_000491"
                     /pseudo
     CDS             1183..3268
                     /locus_tag="VQU28_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            3265..4143
                     /locus_tag="VQU28_000492"
     CDS             3265..4143
                     /locus_tag="VQU28_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HET2475904.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLPNICFYIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVICRNFENFHVSSGKLILHDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLVVKRRD"
     gene            4183..5058
                     /locus_tag="VQU28_000493"
     CDS             4183..5058
                     /locus_tag="VQU28_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2475905.1"
                     /translation="MENELISIVVPIYNVENYLRQCLDSIQKQTYPNFECIMVNDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            5087..6097
                     /locus_tag="VQU28_000494"
     CDS             5087..6097
                     /locus_tag="VQU28_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HET2475906.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTYQNDGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            6107..8551
                     /locus_tag="VQU28_000495"
     CDS             6107..8551
                     /locus_tag="VQU28_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HET2475907.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYTRYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELDKEFNLQVGHDVTAFYNKWENYFNE
                     LVPPSIIHFVSYRKPWTTLIANRYRDLWWEFHDLEWTKILQHHIGEFELTSSLDKEFS
                     CLTLTNSQDLEGIEELVTALPDVVFHIAAWTDMGDKLIKLAVYDNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEINMLAGHSIPYQDYDEELVSIMRDIIGQESREDLVVCLPDAFTDRFRFTS
                     WAIPFWKDHMDHYMDFYRELCSDSWYGSTFVSRPYIDFEDKSQAKAQFEKLKSIWENR
                     DLLIVEGATSRSGVGNDLFDEANSIKRIICPSHSAFSRVHEIEQEIEKYAGGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEYNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            8582..9802
                     /locus_tag="VQU28_000496"
     CDS             8582..9802
                     /locus_tag="VQU28_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HET2475908.1"
                     /translation="MRNTKRAVAFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            9811..11007
                     /locus_tag="VQU28_000497"
     CDS             9811..11007
                     /locus_tag="VQU28_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HET2475909.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            11007..11249
                     /locus_tag="VQU28_000498"
     CDS             11007..11249
                     /locus_tag="VQU28_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2475910.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGQHKLKIWGLV"
     gene            11234..11911
                     /locus_tag="VQU28_000499"
     CDS             11234..11911
                     /locus_tag="VQU28_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HET2475911.1"
                     /translation="MGISVAKGEYITIVDSDDIVKENMIETLYQQVQEKDADVVIGNY
                     YNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNSSAFILPTFKLIKKELFN
                     EVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSGSIMRTEFDLSWARDIVE
                     VFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTDTEEYKDICFRLKLFFDA
                     EQRNGKS"
     gene            11968..13185
                     /gene="secY2"
                     /locus_tag="VQU28_000500"
     CDS             11968..13185
                     /gene="secY2"
                     /locus_tag="VQU28_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HET2475912.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYFYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYKPLI"
     gene            13195..14775
                     /gene="asp1"
                     /locus_tag="VQU28_000501"
     CDS             13195..14775
                     /gene="asp1"
                     /locus_tag="VQU28_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HET2475913.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFEPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            14759..16294
                     /gene="asp2"
                     /locus_tag="VQU28_000502"
     CDS             14759..16294
                     /gene="asp2"
                     /locus_tag="VQU28_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HET2475914.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKTLREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            16291..16731
                     /gene="asp3"
                     /locus_tag="VQU28_000503"
     CDS             16291..16731
                     /gene="asp3"
                     /locus_tag="VQU28_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HET2475915.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            16724..19096
                     /gene="secA2"
                     /locus_tag="VQU28_000504"
     CDS             16724..19096
                     /gene="secA2"
                     /locus_tag="VQU28_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HET2475916.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSSLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            19108..20619
                     /gene="gtfA"
                     /locus_tag="VQU28_000505"
     CDS             19108..20619
                     /gene="gtfA"
                     /locus_tag="VQU28_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HET2475917.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            20612..21949
                     /gene="gtfB"
                     /locus_tag="VQU28_000506"
     CDS             20612..21949
                     /gene="gtfB"
                     /locus_tag="VQU28_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HET2475918.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYVDGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKATYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPEH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQEIMQTVLGG"
     gene            21951..22130
                     /gene="asp4"
                     /locus_tag="VQU28_000507"
     CDS             21951..22130
                     /gene="asp4"
                     /locus_tag="VQU28_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HET2475919.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            22135..22359
                     /locus_tag="VQU28_000508"
     CDS             22135..22359
                     /locus_tag="VQU28_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HET2475920.1"
                     /translation="MIEMLIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            22414..22494
                     /locus_tag="VQU28_000509"
                     /pseudo
     CDS             22414..22494
                     /locus_tag="VQU28_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(22730..23683)
                     /locus_tag="VQU28_000510"
     CDS             complement(22730..23683)
                     /locus_tag="VQU28_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2475921.1"
                     /translation="MSISQRTTKLILATCLACLFAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(23751..24960)
                     /gene="sstT"
                     /locus_tag="VQU28_000511"
                     /pseudo
     CDS             complement(23751..24960)
                     /gene="sstT"
                     /locus_tag="VQU28_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            25099..25659
                     /locus_tag="VQU28_000512"
     CDS             25099..25659
                     /locus_tag="VQU28_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HET2475922.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            25716..26624
                     /locus_tag="VQU28_000513"
     CDS             25716..26624
                     /locus_tag="VQU28_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2475923.1"
                     /translation="MVLEKQLGHGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLESHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            26576..27166
                     /locus_tag="VQU28_000514"
     CDS             26576..27166
                     /locus_tag="VQU28_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HET2475924.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            27169..28275
                     /gene="yqeH"
                     /locus_tag="VQU28_000515"
     CDS             27169..28275
                     /gene="yqeH"
                     /locus_tag="VQU28_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HET2475925.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGTAKVAVWAPEGVAVATRKAII"
     gene            28489..28800
                     /gene="yhbY"
                     /locus_tag="VQU28_000516"
     CDS             28489..28800
                     /gene="yhbY"
                     /locus_tag="VQU28_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HET2475926.1"
                     /translation="MSLASKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            28852..29481
                     /locus_tag="VQU28_000517"
     CDS             28852..29481
                     /locus_tag="VQU28_000517"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HET2475927.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKV
                     GTSYPVIWVDVPLMDISSSMVRDFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            29481..30074
                     /gene="yqeK"
                     /locus_tag="VQU28_000518"
     CDS             29481..30074
                     /gene="yqeK"
                     /locus_tag="VQU28_000518"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HET2475928.1"
                     /translation="MSYQDYINCSREALLEKMAEFLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSNQEFLALIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRAFPGVDVAREIANLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            30075..30575
                     /locus_tag="VQU28_000519"
     CDS             30075..30575
                     /locus_tag="VQU28_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2475929.1"
                     /translation="MKTAFIIIDVQNILVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            30588..30941
                     /gene="rsfS"
                     /locus_tag="VQU28_000520"
     CDS             30588..30941
                     /gene="rsfS"
                     /locus_tag="VQU28_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HET2475930.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            31261..32001
                     /locus_tag="VQU28_000521"
     CDS             31261..32001
                     /locus_tag="VQU28_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001823113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2475931.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            32011..33108
                     /locus_tag="VQU28_000522"
     CDS             32011..33108
                     /locus_tag="VQU28_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HET2475932.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVLD
                     YQKIADLYTEKGAEMEKFVKNLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKVRQHLKSLKGQVNLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAEQNFGRVPIRIETN"
     gene            33346..33600
                     /locus_tag="VQU28_000523"
     CDS             33346..33600
                     /locus_tag="VQU28_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HET2475933.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            33604..33858
                     /locus_tag="VQU28_000524"
                     /pseudo
     CDS             33604..33858
                     /locus_tag="VQU28_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
     gene            34049..35662
                     /locus_tag="VQU28_000525"
     CDS             34049..35662
                     /locus_tag="VQU28_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HET2475934.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            35792..36418
                     /gene="gmk"
                     /locus_tag="VQU28_000526"
     CDS             35792..36418
                     /gene="gmk"
                     /locus_tag="VQU28_000526"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HET2475935.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            36443..36757
                     /locus_tag="VQU28_000527"
     CDS             36443..36757
                     /locus_tag="VQU28_000527"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HET2475936.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            36823..39219
                     /locus_tag="VQU28_000528"
     CDS             36823..39219
                     /locus_tag="VQU28_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HET2475937.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGTVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            39232..40167
                     /locus_tag="VQU28_000529"
     CDS             39232..40167
                     /locus_tag="VQU28_000529"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HET2475938.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEELMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            40160..41473
                     /gene="rsmB"
                     /locus_tag="VQU28_000530"
     CDS             40160..41473
                     /gene="rsmB"
                     /locus_tag="VQU28_000530"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HET2475939.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANNSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            41488..42228
                     /locus_tag="VQU28_000531"
     CDS             41488..42228
                     /locus_tag="VQU28_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HET2475940.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVFMNEEDAE"
     gene            42225..44204
                     /gene="pknB"
                     /locus_tag="VQU28_000532"
     CDS             42225..44204
                     /gene="pknB"
                     /locus_tag="VQU28_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HET2475941.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            44319..45077
                     /locus_tag="VQU28_000533"
     CDS             44319..45077
                     /locus_tag="VQU28_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2475942.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            45079..45528
                     /locus_tag="VQU28_000534"
     CDS             45079..45528
                     /locus_tag="VQU28_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HET2475943.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            45590..45835
                     /locus_tag="VQU28_000535"
                     /pseudo
     CDS             45590..45835
                     /locus_tag="VQU28_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT        13968 a         8215 c        10294 g        13358 t
ORIGIN      
        1 tcaaccagtg cgtcagcttc cgcatcaacg agtgcgtctg aatcggcatc aacctcagca
       61 agtacaagtg catcggcttc agcaagcacc agtgcttcgg cttcagcaag cacgtcagca
      121 tcaacaagca caagtacatc ggcaagcacg tcagcatcga caagtgcaag cacatcggcg
      181 tcaacaagtg caagtacgtc agcatctgaa tcggcctcaa ccagtgcgtc gacttcagca
      241 agtacaagcg cctcagcctc agcaagtatc tcagcgtctg aatcggcatc aaccagcgcc
      301 tcagcctcag catcaacaag tgcatcagct tcagcaagca cctcagcgtc tgaatcggca
      361 tcaacgagtg cctcagcttc agcaagcacc agtacctcag cttctgaatc ggcctcaacc
      421 agtgcctcag cttcagcgtc aaccagtgcc tcagcttctg catcaaccag cgcctcggcc
      481 tcagcaagca cctcagcatc tgaatccgca tcaacgagta cctcagcttc tgaatcggcc
      541 tcaacaagtg cctcggcctc agcaagcaca agcgcctcag cttcagcaag taccagtgcg
      601 tcagcttccg catcaacgag tgcttcggct tcagcctcaa cgagtgcctc agcttcagcg
      661 tcaaccagtg cttcagcctc agcatcaacg agtgcttcgg cttcagcaag taccagtgct
      721 tcggcctcag catcgacaag tgcctcagct tcagcaagta ctagcgcctc agcctcagca
      781 tcaaccagtg cctcggcttc agcaagtact agcgcctcag cttcagcgtc aaccagtgcc
      841 tcagcatcag caagcacctc agcgtctgaa tcggcatcaa ccagtgcgtc tgagtcagca
      901 tcaacgagtg cgtcagcctc agcaagtaca tcagtttcaa attcagcaaa ccattcgaac
      961 tcacaagttg gaaatgcttc tggatcgaca ggtaaatccc aaaaagaatt gcctaataca
     1021 ggtactgagt cgtcaattgg atctgtgtta cttggagttc tagcagctgt tacaggtatt
     1081 ggattggttg cgaaacgccg taaacgtgat gaagaagagt aagacaacct gtaaagttag
     1141 gctaaactaa ctcgcgcaca taaatcaagg agaaaattgc tagtggatga taaaattacg
     1201 gtcattgtac cagtatacaa tgtagaaaac tatctgagga aatgtctaga tagtattatt
     1261 tctcaaacat ataaaaatat tgagattgtt gtcgttaatg atggttctac ggatgcttca
     1321 ggtgaaattt gtaaagaatt ttcagaaatg gatcaccgaa ttctctatat agaacaagaa
     1381 aatgctggta tttctgccgc acgaaacacc ggtctgaata atatgtccgg aaattatgtg
     1441 acctttgtgg attcagatga ttggattgag ttagattatg tagaaaccct atataaaaaa
     1501 ataacagaat atcaagctga tattgcagtt gggaattact attctttcaa cgaaagtgaa
     1561 ggaatgttct actttcatat atcaggagac tcctattatg ataaggtata tgacaatgtt
     1621 tccatctttg agaacttgtt atgaaactca agaaatgaag agttttgctt tgatatctgc
     1681 ttgtggtaaa ctctataagg caagattgtt tgagcagttg cgctttgaca tgggtaaatt
     1741 aggagaagat ggttacctca atcaaaaggt atatttatta tcggaaaagg taatttattt
     1801 aaataaaggt ctttatgctt atcggattag aaaaggtagt ctatcaagag tttggacaga
     1861 aaagtggatg cacgccttag ttgatgctat gtctgaacgt attacgctac tagctaatat
     1921 gggatatcct ctagagaaac acttggcagt ttatcgtcag atgttggaag tcagtctcgc
     1981 caacggtcaa gctagtggtt tatctgacac agcaatatat aaagagtttg aaatgaaaaa
     2041 aagactttta aatcagttaa cggtacaaga atcaagtgaa aagaaagcca ttgtcctagc
     2101 agcaaactat ggctatgtag accaagtttt aacgacaatc aagtctattt gttatcataa
     2161 tcgttcgatt cgtttttatc tgattcatag cgattttcca aatgaatgga ttaagcaatt
     2221 aaataagcgc ttagagaagt ttgactcaga aattattaat tgtcgggtaa cttctgagca
     2281 aatttcatgt tataaaacgg atattagtta cacagttttt ttacgctatt tcatagctga
     2341 tttcgtgcaa gaagataagg ccctctactt ggactgtgat ttacttgtaa cgaaaaatct
     2401 tgatgatttg tttgctacag acttacaaga ttatcctttg gctgctgtta gagattttgg
     2461 gggcagagct tattttggtc aagaaatctt taatgccggt gttctcttgg taaacaatgc
     2521 tttttggaaa aaagagaata tgacccaaaa attaattgat gtaaccaatg aatggcatga
     2581 taaggtggat caggcagatc agagcatctt gaatatgctt tttgaacata aatggttgga
     2641 attggacttt gattataatc atattgtcat tcataaacag tttgctgatt atcaattgcc
     2701 tgagggtcag gattatcctg ctattattca ctatctttct catcggaaac cgtggaaaga
     2761 tttggcggcc caaacctatc gtgaagtttg gtggtactat catgggcttg aatggacaga
     2821 attgggacaa aaccatcatt tacatccatt acaaagatct cacatctatc caataaagga
     2881 acctttcact tgtctaatct atactgcctc agaccatatt gaacaaattg agacattggt
     2941 tcaatccttg cctgatattc agtttaagat agcagctaga gtaatagtta gtgatcgatt
     3001 ggctcagatg acaatttatc caaacgtgac tatatttaac ggaattcact atttggtaga
     3061 tgtcgataat gaattggtag aaaccagtca agtactttta gatattaatc atggtgaaaa
     3121 aacagaggaa attatcaatc aatttgcaag acttggaaaa actattttgt cctttgaaaa
     3181 taccaaaacc tatgaagcag gtcaggaggt atatgctgtt gaccaagttc aagcaatgat
     3241 tgagaaattg agagaaataa gcaaatgaag aaaaatcatt tagtaggaga tgctctgatt
     3301 ttgactgtta gtgatcagat tgaagagttg gattatctcc ttgaatcctt gccaaatatc
     3361 tgtttttata ttgcagcacc tgttcaattt tccgagaaaa tccgtagctt agaaacgaac
     3421 tataatgtcc gtctccgaac gattacaaat gaagaacagc taaactttct agtcgatacc
     3481 tgtgactttt tactagatat caatcacttt caagaagtag atgctattgt ctccaagttt
     3541 gtacaggctg gaaaatctgt ctttgctttt gacaatacag tccatggaaa tcaggggcag
     3601 gaagtttttc tatcaagtac accagacaaa ttggtttcta gagtgagaga ctatctgaat
     3661 gaagtcagag taggcaccaa tcatcaagaa aaaatcatcc aagatgggac ttggaatgtc
     3721 tttaaaatcg atgataaggc tcacttcatt gtaggagcaa atgtcatctg cagaaatttt
     3781 gaaaattttc atgtctcttc tggtaagtta atactgcatg atggtgtttt tatcaacaac
     3841 tcttgtagtt ttaactgtat ggaaagaatt gaaatcggag ctggtaccat gatgggagag
     3901 ggtgttcgtt tttatgatca cgaccatatt tatactgctg aaaagattga aaagtggcag
     3961 tggactacag cgccgattag agttggcaga gactgctgga taggaagtaa tgtcacgatt
     4021 ttaaaaggag tgacgattgg ggataatacc attattggag caggttgtct catacgcaat
     4081 gatattccat caaacagtgt agtttataat aatggaaatc ttgttgtaaa gagaagagat
     4141 taagagggaa atagaataaa tcaagtctag gaaagttagt atatggaaaa tgaactaatt
     4201 agtattgtag tcccaatcta caacgttgag aattatttgc gacagtgttt ggatagtatt
     4261 caaaaacaaa cttatccgaa ttttgaatgt atcatggtta atgatggctc tccagatcat
     4321 tcatccaaaa tatgtgaaga atttgtagag aaagattctc gtttcaaata ttttgagaaa
     4381 gcaaacggcg gtctttcatc agctcgtaac ctaggtattg aatgttcggg gggggcgtac
     4441 attacttttg tagactctga tgattggttg gaacatgatg ctttagaccg attatatggt
     4501 gctttgaaaa aggaaaacgc agatattagt atcgggcgtt ataattctta tgatgaaaca
     4561 cgctatgtgt atatgactta tgttacggat ccagatgatt ctctggaagt gatagaaggt
     4621 aaagcaatca tggataggga aggtgtcgaa gaagtcagaa atgggaactg gactgtagct
     4681 gtcttgaagt tattcaagag agagttacta caagatttac catttcctat aggaaaaatt
     4741 gcagaggata cttactggac atggaaggta cttctaagag cttcgaggat agtctatttg
     4801 aatcgttgtg tttactggta ccgtgttggt ttatctgata ctttatcgaa tacatggagt
     4861 gaaaagcgta tgtatgatga aatcggggct agggaagaaa agatagctat tttagcaagt
     4921 tcagactatg acttgaccaa tcatattttg atttataaaa atagattaca aagagtgata
     4981 gcaaaattag aagaacaaaa tatgcagttc acagagattt atagaagaat gatggaaaaa
     5041 ttgtctttac ttccgtagat agtaataaaa aatgagatag cgtaatatga aactacattt
     5101 aacaaattta tacggcatgg ctggtgatag tacggttatc ttagctcaaa atgctgttca
     5161 aaagatagct agtcaactgg gatttagaga ggttggtatt tatttttaca acattgcttc
     5221 agatagtcct tctgaaatga ataagcgtct ggatggtatt atggccagta tttctattgg
     5281 ggatatttta gtctttcagt ctccaacctg gaatggcttt gaatttgatc gtctcttgtt
     5341 tgataagttg aaggatatgc aggtgaaaat tatttgcttt atccatgatg ttgttcccct
     5401 catgtttgat agtaactatt atctcatgaa agactatatg tacatgtata atctatcaga
     5461 tgttttgata gtgccgtcag agagaatgaa aacacgcctg atggaagaag gattgacgac
     5521 taagaagatt cttgttcaag ggatgtggga tcatcctcat gatttatcct tatacacccc
     5581 tgcttttaaa aaagaacttt tttttgctgg aagtttagag cgttttccag acttacaaaa
     5641 ttggtctcaa gatacgcctt tgagagtatt ttcaaataaa ggggaagcta gttctagtgc
     5701 tagaaatctc agcatcgaag gatggaaaaa agatgaggaa ttgttgctag aattatcaaa
     5761 gggtggattt ggccttgtct ggggaactta tcaaaacgat ggagaaagca accaatatta
     5821 taccttaaat atatctcata aggtgagtac ctatctgaca gcgggcatcc cagtcattgt
     5881 accaagtagc ttgtcaactg ctaaatttat agtagatcaa ggcttaggct ttgtggcgaa
     5941 tagtctagaa gaagttcatg cgatagttga taaaatgaat ctacaagaat atcaagaaat
     6001 gacgaatcgg ataaaaacct ttagttattt gctaaaagaa ggttatttta ctaaaaaatt
     6061 attggtagat gcaatttacc atttgggagt tgaataagag aacaagatga ttaaaacaat
     6121 tgtattagct ggtgatcgta attacatcag gcagttagaa acaaccataa aatctatttt
     6181 ataccacaat cgagatgtta agatttatat tttaaatcaa gatattatgc cagattggtt
     6241 tcgcaaacca cgaaaaatag ctcgcatgtt aggtagtgag attatcgatg tgaagctacc
     6301 tgaacaaact gtgtttcaag attgggaaaa gcaagatcac attagtagca ttacctatac
     6361 tagatatttt attgcagatt atatccaaga agataaggtt ttatatttag acagtgattt
     6421 gattgtaaat acttctttag agaaattatt tagtatttgt ttagaagaaa aatcactcgc
     6481 agcagttaaa gatacagatg gaattacatt taatgcaggt gttttattaa tcaacaataa
     6541 aaaatggcgt caagagaaat taaaagaacg actaattgaa cagagcattg ttacaatgaa
     6601 ggaagttgaa gaaggccgtt tcgagcattt taatggtgat caaacgattt ttaatcaggt
     6661 cttacaagat gattggttag agctggataa agaatttaac cttcaagtgg gtcatgatgt
     6721 aactgctttt tataataaat gggaaaatta ttttaatgag ttagtacctc ctagtattat
     6781 tcattttgtg tcttacagaa aaccctggac caccttgata gccaatcgtt atcgtgattt
     6841 atggtgggaa tttcatgatt tggaatggac taaaatttta caacatcata taggggagtt
     6901 tgaactgaca tcgtctttag ataaggaatt ttcttgttta actttgacaa attcccaaga
     6961 tttagaagga atagaagagt tagtcacagc tttaccagat gtggtatttc atattgctgc
     7021 ttggacggat atgggtgata agctgataaa attagctgtg tatgataatg tgagattgca
     7081 tccacaaatt gttcctcctg tattagataa gctgaaaaag tcaacaaatc tatatttgga
     7141 tatcaatcat ggtagtgcag atgagaactt tttaaaatct ttgcaagaac aagaaaaaac
     7201 gctactagct tttcaatcga ctcagcacgg agagttagga caaatcgttt tcgaaaatgg
     7261 gaaagtttcc tttatgattg atacgattaa agattttaag aaaaacggac atcttacctg
     7321 ttttcgacaa cttccaagtt taacttgttt aacgtttacg gcttctcagg atatcgaaca
     7381 attggattac ttggctggac agttgccaaa tgttgttttt caagttgctg cttggacagc
     7441 tatggggcca aaattatatg atttgtctaa tcgttatcct aatattcagc tctatccggc
     7501 aatttctaga gataagctag acgagttgaa ggagaagatg gatgcttatt tagatatcaa
     7561 cctactgact tcaacatccg atatcgttgc agaaatggct catctatcta aacctatact
     7621 agccttttat aaatctcaaa atgggaataa tggccaaagg ttgtattcaa gtgaacatcc
     7681 tgaacgaatg ttggctgatt tgcaaaaatt gataactaag gatatgctag aaaaaccgct
     7741 tgatataatc caggtgaaag ggatagatga aaccttggat tatattattg aacacaactc
     7801 ttctttagtt cgttttggag atggggaaat caatatgctt gcagggcatt caattcccta
     7861 ccaggattat gatgaagagt tggtttcaat catgagggac attatcggcc aagaaagtcg
     7921 agaagattta gtagtgtgcc ttcctgatgc ttttacagat cgttttaggt ttacatcgtg
     7981 ggcgattcca ttttggaaag atcacatgga tcattatatg gatttttaca gagagttatg
     8041 cagtgattca tggtatggct caacctttgt atctcgccct tatatcgatt ttgaagacaa
     8101 gagtcaagct aaagctcaat ttgaaaaatt gaaaagcatt tgggaaaacc gtgacttact
     8161 gatagtcgaa ggtgcgactt ctcgttcagg tgtcggaaat gatttattcg atgaggcaaa
     8221 ttctattaag cgaattatct gtccttctca tagtgccttt tctagagttc atgaaattga
     8281 acaagaaatt gaaaagtatg ctggtggtcg cttgatttta tgtatgctgg gtcctacagc
     8341 aaaagttctg agttataatc tatgccagat gggctatcaa gttttggatg taggccatat
     8401 tgactcagag tatgaatgga tgaaaatggg agctaaaact aaggttaaat tttctcataa
     8461 acatactgca gagtataatt ttgatcaaga tattgaattt attgatgatg aaacctataa
     8521 cagtcagatt gttgcacgaa tattaaacta gactatttaa aataaatgat aaggatttaa
     8581 aatgagaaat accaaacgcg ctgtagcatt tgcaggtgat tacgcttata ttcgacaaat
     8641 cgaaacggcg atgaagtcac tctgtagaca caatagtcat ttgaaaattt atctgctaaa
     8701 tcaggacatt cctcaggaat ggtttagtca aataagaata tatttacaag agatgggggg
     8761 cgacttgatt gactgcaagt taattggctc acagtttcaa atgaattggt ctaataaatt
     8821 acctcatatc aatcatatga catttgcacg ctattttatt ccagattttg taacagaaga
     8881 taaagttctc tatctagata gtgatttgat tgtgactggt gatttgaccg atttgtttga
     8941 attagactta ggtgaaaatt atttggcagc agctcgttct tgctttggag caggagtcgg
     9001 cttcaatgct ggtgttctct tgattaacaa caaaaaatgg ggatctgaaa ctattcgaca
     9061 aaaattgatt gacttaacag aaaaagaaca tgagaatgtg gaagaaggag accagtcaat
     9121 tttgaatatg ttgtttaaag atcaatatag ttcccttgaa gatcaatata attttcaaat
     9181 aggatatgat tatggggcgg cagcctttaa acatcaattc atttttgata ttccgctcga
     9241 accactgcca ctaattttac actatatttc tcaggataag ccttggaatc aattttctgt
     9301 tggacgtcta agagaagttt ggtgggaata ctctttgatg gattggtctg ttattttaaa
     9361 tgaatggttt tcaaagagtg tgaagtatcc tagtaaatca caaatattta agttacaatg
     9421 tgttaattta acgaattctt ggtgtgtcga gaaaatcgat tatttggcgg agcaattgcc
     9481 agaagttcat tttcatattg ttgcttatac aaatatggca aatgaactac tagctttaac
     9541 gcgttttcct aatgttaccg tatatccaaa ttccttacca atgttattgg aacaaatagt
     9601 aatagcttca gatttgtatt tggatttgaa tcatgatcga aaattagaag atgcatatga
     9661 gtttgtgctt aagtacaaaa aaccaatgat agctttcgac aatacttgct ctgaaaatct
     9721 ttctgagatt tcatatgaag gtatctatcc aagctccatt ccgaaaaaaa tggttgcagc
     9781 aatcagatct tacatgaggt agagaacagt atgagaaaat caatagtatt agcggcagat
     9841 aatgcctatc ttattccttt agagacgact ataaagtctg tattgtatca caatagagat
     9901 gttgattttt atattctcaa cagtgatata gctcctgaat ggtttaaatt attggggaga
     9961 aaaatggaag ttgtgaattc tacaattcgc agtgtacaca ttgataaaga actttttgaa
    10021 agctataaaa caggacctca tataaattat gcttcttact ttagattttt tgcgacagaa
    10081 gtggttgaat ctgatagggt attgtatctg gattccgata tcattgtaac tggggaacta
    10141 gctactttgt ttgagataga tctcaaagga tattcaattg gtgctgttga tgatgtctat
    10201 gcctatgaag gacgaaaatc tggatttaat gctggtatgt tactaatgga tgttgcaaaa
    10261 tggaaagaac actctattgt caatagttta ttggaattag cggccgagca aaatcaagtt
    10321 gttcatcttg gggatcagag tattttaaat atttattttg aggataattg gctagcctta
    10381 gataaaacat ataattatat ggtgggtgtt gatatttatc accttgctca agaatgtgaa
    10441 cgtctagatg acaatccacc tacaattgtt cactatgcta gtcatgataa accttggaat
    10501 acatatagta tatctagact acgtgaatta tggtgggttt atagagattt ggattggtca
    10561 gagattgctt ttcaacgttc cgatttaaat tattttgaaa gaagcaatca gtctaaaaaa
    10621 caagtgatgc ttgtgacatg gagtgcagat ataaaacatt tagagtattt agtacaacgg
    10681 ttacctgatt ggcattttca tttggctgca ccgtgtgatt gttctgagga gctgacctct
    10741 ctatcacagt atacgaatgt aacagtatat caaaatgtat tacatagtag aattgattgg
    10801 ctattggacg attctatagt ttatttagat attaatacag gtggagaggt ttttaatgta
    10861 gttacaaggg cacaagaaag tggcaagaaa atcttcgctt ttgatatcac acgtaaaagt
    10921 atggatgatg gactctatga cggtattttt tctgtggaga gaccagatga tttagtggat
    10981 agaatgaaga atatagagat agagtaatga gtgaattaat tagtgttgtg gtaccgatat
    11041 acaatacggg aaaatattta gtggagtgtg tcgagcatat tctgaagcaa acctatcaaa
    11101 atatagaaat tattttagtt gatgacggtt ctacggataa ttctggggaa atttgtgatg
    11161 cttttatgat gcaagataat cgtgtgcgag tattgcatca agaaaataag ggggggcagc
    11221 acaagctaaa aatatgggga ttagtgtagc taagggagag tacatcacga ttgttgattc
    11281 agatgatatc gtaaaagaaa atatgattga aactctttat cagcaagtcc aagaaaagga
    11341 tgcagatgtt gttataggga attactataa ttatgacgaa agtgacggga atttttattt
    11401 ttatgtaaca gggcaagatt tttgcgtcga agaattagct atacaagaaa ttatgaaccg
    11461 tcaagcagga gattggaaat tcaatagctc ggcctttata ttgccgacat ttaagttgat
    11521 taaaaaagaa ttattcaatg aagttcactt ttcaaatggt cgccgctttg atgatgaagc
    11581 aactatgcat cgcttttatc ttttagcctc taaaatcgtc tttataaacg ataatctcta
    11641 tctgtataga agacgttcag gaagcatcat gagaacggaa tttgatcttt cctgggcaag
    11701 agatattgtt gaagtgtttt ctaagaaaat atcggattgt gtcttggctg gtttggatgt
    11761 ctccgttctg cgtattcgat ttgtcaatct tttaaaagat tataagcaaa ctttagaata
    11821 ccatcaatta acagatactg aggaatataa agatatttgt ttcagattaa agttgttttt
    11881 tgatgcagaa caaagaaatg gtaaaagttg aaataaaaga attgttattt accatatcac
    11941 aaacaatgaa ggtgagggga gtgttttatg actaagattt attcgtcaat agcagtaaaa
    12001 aaaggactat ttaccttatt tctactgttt atctatgtat tgggaagtcg tattactctc
    12061 ccttttgttg acctaaatac taaagatttt ttaggaggtt caacagccta tctagccttc
    12121 tcagccgccc taacaggtgg aaatctaaga agtttgtcaa ttttttctgt tggattgtcc
    12181 ccttggatgt cagctatgat tttatggcag atgttttctt tttctaaacg gttgggttta
    12241 acatctacgt ctatagaaat acaagatcgc cgtaaaatgt acctgacctt gatgatagct
    12301 gtcattcaat cattggcagt tagcttgaga ctgccagtac aatcctccta ttctgcaata
    12361 ttggttgttc taatgaatac aatattgctg atagcaggaa cattttttct tgtttggttg
    12421 tcagatttaa atgcgagtat ggggattgga ggttctatag tcattctcct atccagtatg
    12481 gttttaaata ttcctcagga tgttttggaa acatttcaga cagtacacat tccaacaggg
    12541 attattgtgt tacttgcttt attaaccctt gtcttttctt atttacttgc ccttatgtat
    12601 cgagctcgct atttggttcc tgttaataaa attggcttac acaatcgatt taaacgctat
    12661 ttttatctcg aaatcatgtt gaatcctgca ggtgggatgc cttatatgta tgtgatgagt
    12721 tttcttagtg taccagctta tttgttcatc ttgttgggat ttattttccc taatcattca
    12781 gggttagcgg ctttatcaaa ggaatttatg attggaaagc ctttgtgggt ctatgtttat
    12841 atttcggtct tatttttatt tagtatcatt tttgcttttg ttacgatgaa tggagaagag
    12901 attgcagacc gtatgaaaaa atctggagaa tacatttatg gtatttatcc aggtgcggat
    12961 actagtcgat ttattaatcg attggtcctt cgtttctcag tcataggtgg tctctttaat
    13021 gtggttatgg caggtggtcc catgcttttt gttttgtttg atgaaaagtt attacgattg
    13081 gcaatgattc caggtttgtt tatgatgttt ggaggcatga tttttacgat tagagacgag
    13141 gtcaaggctt taaggctaaa tgagacctat aaacctttga tttaggagac ttttatgtat
    13201 tattttattc cagcttggta tgggtcagaa agaacatggc atgcagatat cactccatgg
    13261 tatttttctc attttcgtct agagtttgat gatacctttc accagatccg gctctttcaa
    13321 gagcaagata tagattctcg tctattagta ttagcttacc agcctcatct acgttatttt
    13381 ttatatagac atggtgtgtt agaaacggat acttattccg tttttgatgt tatgcaagat
    13441 tttcataatc tccataccca agttttaagc attagagata ttgagtggga tgatgactgt
    13501 gaatttattt atagtccctt tacgattatc gttcaaaaaa atgggaagaa atttgctaag
    13561 gttgaacatg gagttgaagg cttcatcagt gatatacagt attttgaacc aaatggtcaa
    13621 atacatatgc accatatcat ggatgatcga gggtttatat cgagtattat tttttttgaa
    13681 gatgggcaag cagcctatca agaatatttg aatcctaagg gagtttggca atttagagag
    13741 cgtttaaaag aaggaggaca ggtagaagtc aatccaattt tgggttatcg ctttaaaatg
    13801 cttacctatc aaaatatggg agatctggtg gcagaatttt ttgagaatta tctgcaaacg
    13861 tatgtgaagg atcaggatat ttttatgctt ccttctcatt ctcatcatga ccagttggta
    13921 ctagatcgtt tacctagtac taatcctaaa ctgttgagtc tgttcattgg acgtaatcct
    13981 caagatacct ttagggattt agatgtaact tttgaaaaat cggatttgat tttggtggat
    14041 agagaggata gtttacgatt gttgcaggag ttgtatcctg aacgaatgca tcaatgttat
    14101 catttatcat cttttgacac ccgattacga ttgggacgaa gccaaactaa gaaagaatcc
    14161 atcatttatt ttcaactgga ttttgagcag gggattgata atcaagctct gcttcaagtc
    14221 ttgtcctttg tcgctgaaaa taaggatact gaggtgattt ttggagcctt tgctgctagt
    14281 caggagcaaa tgaatgaggt tgaagggatt gttgagtctt tcatccaaga aaacattcaa
    14341 tccgaaaatc tgggaaaggc gattgattat ggtgatgcag aaaatcccct ggaagaaaat
    14401 caacaccagg acttacgctt acagtttgtt aacttgaatg atgagttaga tttgataaaa
    14461 acactagaat ttgtccgttt gattgtggat ttaaatagac atcctcatct ctacacacag
    14521 attgctggga ttagtgcagg aattcctcaa atcaacctag ttgaaaccgt ctatgtggaa
    14581 catttaaaaa atggttattt gctgacagat gtgacagaat tttctaaggc tgcacattat
    14641 tacacagata ggttgaagga gtggaatgag tccttgatat attcaattga taagattaag
    14701 gagcacacag gacaacaatt tcttggaaaa ttagagaaat ggatagagga ggttaaaaat
    14761 gtcaaaggaa cttaatattt tacagatagg acttgccaat tgggaaaatc actatgacat
    14821 acctgaaaat atgagttggt attattttta cccaaactca tcaaaaaccc ttcgtgaaat
    14881 aatcgaaaaa gaggatatta accgttttca tgcagtttta atagaagatg gtcagtattc
    14941 cagagactta ttttcctatg taaaatattt tgaaccttat actttgttct ataaccagaa
    15001 tctacaaata aatgatagag aggttgtgga ttttctaaaa aaacgatgtg cacaagcaat
    15061 tgatttttta agtccccaac aactaatcaa tgatttaagt aaatctcttt ttggcggtgg
    15121 gtatggtgat aaactctttc ctccgacgat acaagtcaat ccaaatttta caggagctat
    15181 ttcttatcaa ggattggatt atgtcagttt ggaaggtgag tttgggcaag attttgccca
    15241 gcttgcctat tgggcttata atattatggt gcaaaaaaca ctccctattg agttgtggct
    15301 tgaatatgag aaggaaggca attgtgactt tcgtttagta atccgtaaaa tgtggagtgg
    15361 gtctgttgat gatttctttg aagaagtaat agtatctgaa aaagacttgg agcaagcact
    15421 ttttatggat agtcgagacg gagactactt tctctcgata tctgttgaag caagaggtcg
    15481 tggaactatc aaactaggta atcttcacca acgatggagt cgaaaacaat ttggtaagtt
    15541 tgtacttggt ggaaatatcc tacatgattc caagcgtgat gaaataaact atttcttcca
    15601 tccaggtgat tttaaaccgc ctttgactgt ctattttgca ggttatcgac ctgcagaagg
    15661 attcgagggt tactttatga tgaaaactct tggatgtccc ttcattttat tttctgatcc
    15721 acgtttagag gggggagctt tttatctcgg aacggatgag ctagagggaa aagtaaagga
    15781 tacgatcact cactatcttg attatttagg ctttgatcat aaggatttga ttttatcagg
    15841 tctttctatg ggaacgtttc cggctctcta ttatggtgct tcttttgaac cccatgccat
    15901 catagttggt aagcccttgg ctaatttagg aactatagct agtcgtggac gtttggacgc
    15961 accgggtgtc tctaacttag cttttgattg tttaattcat catacaggtg ggacaagttc
    16021 tcaagatatg acggagttgg atcagcgttt ttggaaaatt tttaaacaag caaatttttc
    16081 aaagacaacc tttggtttat cctatatgaa agatgaagaa atggatccac aagcctatga
    16141 acaattagtg tcttatctgt gtaatacagg tgcgaagatt ttatctaaag gaactgctgg
    16201 acgacacaat gatgatacag ataccaatat ttcttggttt ttgcactttt atagaatggt
    16261 cttagagact ggttttggaa gggagaaaag atgattatta cacagagaca gtctatttat
    16321 tggggagaag ttggtgggac ttatatgtat ggaacaactg tatcttatta ccttgacaaa
    16381 agtgttcgtc tgtataatcc tctattgcca tctggtgaga ttctaaagac ttggttttct
    16441 agtgtcaatt accaggctgc acgaacccaa cctcagcttc ccttattaaa aagaaagcag
    16501 gagtatcaac tatcactggt ttttgattgt cagcctgaaa atggagttta taccaagata
    16561 actttttttg accgctatgg tgatatttta gaaaaaaagg tagaaaaagc gaaagatttc
    16621 atatttactt atccagaaga tagttatact tatcaagttt ctcttttaag tgctggattt
    16681 gagtccttaa ctttttatca tttttctatc aaggagatca gaagtgttta gacgtttagg
    16741 tcaagatttc cagcttagga aagtgaaaaa gattttaaag cagattaatg ccctgaaagg
    16801 caagatgtcc tctctttcgg atcaagaatt agtagctaaa acagtagagt ttcgtcagcg
    16861 tctttccgag ggagaaagtc tagacgatat tttggttgaa gcttttgctg tggtgcgtga
    16921 agcagataag cggattttag ggatgttccc ctatgatgtt caagtcatgg gagctattgt
    16981 catgcactat ggaaatgttg ctgagatgaa tacgggggaa ggtaagacct tgacagctac
    17041 catgcctgtc tatttgaacg ctttttcagg agaaggagtg atggttgtga ctcctaatga
    17101 gtatttatca aagcgtgatg ccgaggaaat gggccaagtt tatcgttttc taggattgac
    17161 cattggtgta ccatttacgg aagatccaaa gaaggaaatg aaagctgaag aaaagaagct
    17221 tatctatgct tcggatatca tctacacaac caatagtagt ttaggttttg attatctaaa
    17281 tgataaccta gcctcgaatg aagaaggtaa gtttttacga ccgtttaact atgttattat
    17341 tgatgaaatt gatgatatct tgcttgatag tgcacaaact cctctgatta ttgcgggttc
    17401 tcctcgtgtt cagtctaatt actatgcgat cattgataca cttgtaacaa ccttggtcga
    17461 aggagaggat tatatcttta aagaggagaa agaggaggtt tggctcacta ctaagggggc
    17521 caagtctgct gagaatttcc tagggattga taatttatac aaggaagagc atgcgtcttt
    17581 tgctcgtcat ttggtttatg cgattcgagc tcataagctc tttactaaag ataaggacta
    17641 tatcattcgt ggaaatgaga tggtactggt tgataaggga acagggcgtc taatggaaat
    17701 gactaaactt caaggaggtc tccatcaggc tattgaagcc aaggaacatg tcaaattatc
    17761 tcctgagacg cgggctatgg cctcgatcac ctatcagagt ctttttaaga tgtttaataa
    17821 gatatctggt atgacaggga caggtaaggt cgcggaaaaa gagtttattg aaacttacaa
    17881 tatgtctgta gtacgcattc caaccaatcg tccgagacaa cggattgact atccagataa
    17941 tctatatatc actttacctg aaaaagtgta tgcatccttg gagtacatca agcaatacca
    18001 tgctaaggga aatcctttac tcgtttttgt aggctcagtt gaaatgtctc aactctattc
    18061 gtctctcttg tttcgtgaag ggattgccca taatgtccta aatgctaata atgcggcgcg
    18121 tgaggctcag attatctccg agtcaggtca gatgggggct gtgacagtgg ctacctctat
    18181 ggcaggacgt ggtacggata tcaagcttgg taaaggagtc gcagagcttg ggggcttgat
    18241 tgttattggg actgagcgga tggaaagtca gcggatcgac ctacaaattc gtggccgttc
    18301 tggtcgtcag ggagatcctg gtatgagtaa attttttgta tccttagagg atgatgttat
    18361 caagaaattt ggtccatctt gggtgcataa aaagtacaaa gactatcagg ttcaagatat
    18421 gactcaaccg gaagtattga aaggtcgtaa ataccggaaa ctagtcgaaa aggctcagca
    18481 tgccagtgat agtgctggac gttcagcacg tcgtcagact ctggagtatg ctgaaagtat
    18541 gaatatacaa cgggatatag tctataaaga gagaaatcgt ctaatagatg gttctcgtga
    18601 cttagaggat gttgttgtgg atatcattga gagatataca gaagaggtag cggctgatca
    18661 ctatgctagt cgtgaattat tgtttcactt tattgtgacc aatattagtt ttcatgttaa
    18721 agaggttcca gattatatag atgtaactga caaaactgca gttcgtagct ttatgaagca
    18781 ggtgattgat aaagaacttt ctgaaaagaa agaattactt aatcaacatg acttatatga
    18841 acagttttta cgactttcac tgcttaaagc cattgatgac aactgggtag agcaggtaga
    18901 ctatctacaa cagctatcca tggctatcgg tggtcaatct gctagtcaga aaaatccaat
    18961 cgtagagtac tatcaagaag cctacgcggg ctttgaagct atgaaagaac aaatccgtgc
    19021 ggatatggtg cgtaatctcc tgatggggct ggttgaggtc actccaaaag gtgaaatcgt
    19081 gactcatttt ccataagagg agaaaatatg acaatttaca atataaattt aggaattggt
    19141 tgggctagta gcggtgttga atacgctcaa gcctatcgtg ctggtgtttt tcggaaatta
    19201 aatctgtcct ctaagtttat ctttacagat atgattttag ccgataatat tcagcactta
    19261 acagccaata ttggttttga tgataatcag gttatctggc tttataatca tttcacagat
    19321 atcaaaattg cacctactag cgtgacagtg gatgatgtct tggcttactt tggtggtgaa
    19381 gaaagtcaca gagaaaaaaa tggcaaggtt ttacgtgtat tcttttttga ccaagataag
    19441 tttgtaacct gttatttggt tgatgagaac aaggacttgg ttcaacatgc cgagtatgtt
    19501 tttaagggaa acctgattcg gaaggattac ttttcttata cgcgttattg tagcgagtat
    19561 tttgctccca aggacaatgt tgcagtctta taccaacgaa ctttttacaa tgaagacggg
    19621 actccagtct atgatatttt gatgaatcaa gggaaggaag aagtttatca tttcaaggat
    19681 aagattttct atggaaagca agcttttgtg cgtgccttta tgaaatcttt gaatttgaac
    19741 aagtctgatt tggttattct ggatagggag acaggtattg gacaggttgt gtttgaggaa
    19801 gcacagacag cacatctagc ggtagttgtt catgcggagc attatagtga aaatgctaca
    19861 aatgaggact atatcctttg gaataactat tatgactatc agtttaccaa tgcagataag
    19921 gttgacttct ttatcgtgtc tactgataga caaaatgaag ttctacaaga gcaatttgcc
    19981 aaatatactc agcatcagcc aaagattgtt accattcctg taggcagtat tgattccttg
    20041 acagattcaa gtcaagggcg caaaccattt tcattgatta cggcttcacg tcttgccaaa
    20101 gaaaagcaca ttgattggct tgtgaaagct gtgattgaag ctcataagga gttaccggaa
    20161 ctaacctttg atatctatgg tagtggtgga gaagattctc tgcttagaga aattattgca
    20221 aatcatcagg cagaggacta tatccaactc aaggggcatg cggaactttc gcagatttat
    20281 agccagtatg aggtctactt aacggcttct accagcgaag gatttggtct gaccttgatg
    20341 gaagctattg gttcaggtct acctctaatt ggttttgatg tgccttatgg taatcagacc
    20401 tttatagagg atgggcaaaa tggttatttg attccaagtt catctgacca tgtagaagac
    20461 caaatcaagc aagcttatgc cgctaagatt tgtcaattgt atcaagaaaa tcgtttggaa
    20521 gctatgcgtg cctattctta tcaaattgca gaaggcttct tgaccaaaga aattttagaa
    20581 aagtggaaga aaacagtaga ggaggtgctc catgattgaa ctttatgata gttacagtca
    20641 agaaagtaga gatttacatg aaagtctagt cgctactggt ctttctcaac ttggagtggt
    20701 catcgatgca gatggttttc tgcctgatgg tctgctttct ccttttacct attatctagg
    20761 ttacgaggat ggaaaacctc tctattttaa ccaagttccc gtttcagatt tttgggaaat
    20821 tttaggagat aatcagtctg cttgtattga agatgtgacg caggagaggg ctgtcattca
    20881 ttatgttgat ggaatgcagg ctcgcttggt taaacaggta gactggaaag acctagaagg
    20941 tcgagtacgt caggttgacc actacaatcg ctttggtgct tgctttgcta aaaccactta
    21001 tagcgcagat agcgagccga ttatgacagt ttaccaagat gtcaatggcc aacaagtttt
    21061 actggaaaac catgtgacgg gtgatatctt attgactttg cctggtcagt ccatgcgtta
    21121 ctttgcaaat aaagttgaat ttatcacctt ctttttgcaa gatttggaaa tagataccag
    21181 tcagcttatc tttaatactc tagcgactcc tttcttggtt tccttccatc atccagataa
    21241 atctggctcg gatgtcttgg tatggcagga acctctctat gatgccattc caggtaatat
    21301 gcagttgatt ttggaaagtg ataatgtgcg tactaagaag atcatcattc caaataaggc
    21361 gacttatgag cgcgctttag agttaactga cgagaaatac catgatcagt ttgtgcactt
    21421 gggttatcat taccagttca aacgtgataa tttcctaaga cgagatgcct taatcttgac
    21481 caattcagat cagattgagc aagtagaagc aatcgtagaa gccttgcctg atgttacctt
    21541 ccgtatcgca gcagtgacag agatgtcttc taagctctta gacatgcttc gctatcctaa
    21601 tgtggtcctt taccagaatg ctagtccaca gaagattcag gagctgtatc aactgtcgga
    21661 tatttacttg gatatcaatc acagcaatga gttgttgcag gcagtgcgtc aggcctttga
    21721 gcacaatctc ttgattcttg gatttaacca aacggttcac aatagacttt atatcgctcc
    21781 agaacatcta tttgaaagta gtgaagttgc tgccttggtt gagaccatta aattggccct
    21841 ttcagatgtt gatcaaatgc gtcaggcact tggcaaacaa ggccaacatg caaattatgt
    21901 tgacttggtg cgatatcaag aaataatgca aactgtttta ggaggctaac atgtcagagg
    21961 aagatttatt ttacaaagac gttgaaggtc gcatggaaga gttgaaacaa aaacccatca
    22021 agaaggaaaa agaaacccga ggggagaaga ttagtaaaac tttttcactt ttactgggct
    22081 taatgattct gattggtttg ctctttactt tgttgggaat tttgaggtag atcaatgatt
    22141 gaaatgctaa ttgttttagc tattatccta tctcttgctt tgattgtatt ggtaactata
    22201 caaccccgtc aaaatcaact attttccatg gatgccacta gtaatattgg taaaccaagc
    22261 tactggcaga gcaacacctt ggtcaaggtg ctcactttat tggtgagttt ggctttattt
    22321 gttctactat taacctttat ggtgattact tataaataaa agaaaacttc agatattcac
    22381 cttttgtgga ttggtctgaa gttttctttt ttatactcaa tgaaaatcaa agagcaaact
    22441 aggaagctag ccgcaggctg ctcaaaacac tgttttaagg ttgtggatgg aactgacgaa
    22501 gtcagctcaa aacaccgttt tgaagttgta gatagaactg acgaagtcag ctcaaaacac
    22561 cgttttgagg ttgtggatag aactgacgaa gtcagctcaa aacaccgttt tgaggttgtg
    22621 gatagaactg acgaagtcag ctcaaaacat cgttttgagg ttgtggatag aactgacgaa
    22681 gtcagtaaca tatatacagc aaggcgacgc tgacgtggtt tgaagagtat tactgtctat
    22741 atttttggta aaaatcaact tttacttgga tgaaggtttt ggcttcacgt aggagttgaa
    22801 gaagggtggc gcgggtttca aattcttctc ttgtcttggg cagactgcgg ttccggaaga
    22861 cttccagata acgttcaatt tcatctagca aatcagaagc aggattggtc tggctcagtt
    22921 gacctgcaat ttttgaaaag agttgcgcta agatcaggct ttcactggcg gcaaggtgac
    22981 aagtgttaat ctgttgggcc atgtttctca ggatacgact ttgtcgttgt ctcatctcaa
    23041 agtagtggat atggtagtcg gtctggtgaa agaggtggtc agagtgatcc aaatagacca
    23101 gtctgagggc ttctttcaaa agcgtgtcta attctgctac cagctgtgct cggttgcgtc
    23161 cgtctcctct ggataaatag tatttgaagc gctggaggat atcttttaac ttttcttcca
    23221 ccagcgtgtg gtagtgctgg atttcctctt ctcgtgaagg catatagaga ttaacaagca
    23281 aggcaaatcc tgtaccgata gcaaagagaa ggaattcgtt gactaggagg tctggagagg
    23341 ttgactcttg aaccaagaga tggctaacca aaaccgtgct tggtgtgatg ccaatttccc
    23401 agcccatctt gtaggctaaa ggaacgtaga aggccagata gaggccgaga ctccagatat
    23461 gaaatccgct caagtgaaaa gctagaacac cgatagccag agctagaagc atagaaaaaa
    23521 gacgattgcg agccagtttt aaagtacttc tacgcgtatc agataggctc aagagagcga
    23581 taattccagc cgaaactgct gacgaaagat tgagaaaata agcaaacagg caggcaagac
    23641 aggtagctaa gatgagcttg gtcgtacgtt ggctaataga cataagaatt tcctaataag
    23701 ttagaataaa agcgtaaaag acaagacatg agcaggcttg ccttgatgag ttatttttta
    23761 cgggttgctg cgtattcggc aacggcggta aagaggacat ctgtagaaga gttaagggct
    23821 gtttcacatg agtcttggat gacaccaatc acaaaaccaa ccccaacaat ttgtatggca
    23881 atatcgttag aaataccgaa aaggctacaa gcaactggga taagaaggag ggaacctccg
    23941 gcaatacctg aagcaccaca ggctgagata gctgctacca cactgaggac aaaggctgtg
    24001 gcaaagtcaa caggaattcc aagagtgtta actgtaacaa gggtcaaaag gttaatggta
    24061 atcgctactc cagccatatt gatagtagaa ccgagtggga tagaaacaga ataggtatct
    24121 gggttgagtc caaggtcatg gcagagtttc atattgacag gaatgttagc cgcagaacta
    24181 cgagtgaaaa aggctgtcac accgctgaca cggaggcagt tccaaactag agggtaagga
    24241 ttgcgtctca taaagaagaa ggcaatcaaa gggttgacca caggggcaac aaaaagcata
    24301 gtcgttacta atagaaccaa taaaataccg tagttggcaa gccttccgac tcccttgtca
    24361 gaaatggttt taaaaacaag accaaggatt ccaaatggag tcagattgat gatccattcg
    24421 acaattttag aagtcacgtc agcgatagtt tttagcaatt ctttactatt tttactggct
    24481 tctctcatag cgattccaaa aatgactgcc caagataaga ttccaatata gttagcagta
    24541 agcagggcgt tgactgggct gtcaaccagt ttgagcaaga ggttgctgag gacctgccca
    24601 atcccatctg gtggtgcaat ttcagtattg gcactattta gggtaatttc aatagggacg
    24661 atgaaacttg ctagtacagc tacaagagca gcggcgaaag tccctatcat aggatataca
    24721 agaaaacaac agttttcata ttgctatctt gtcccttttg atgttgggaa agggcattgg
    24781 caacgagagc aaagactagg ataggagcaa cagcttttag acctccaacg aataaatccc
    24841 cgagtagccc aatccctgag agattaggaa gggtcagtcc taggattccc cacaaagcat
    24901 accaatcaag atacgcttga caaggcttgc cttattccaa gcatgaatga ttcttttcat
    24961 aataatctcc tttttgtgta gtgattatga ttatagtata aatgatagac aaaatcaaga
    25021 attttctgtc tattttttga atatttatgg agaatgagac tgatgaaaat atggtataat
    25081 gaaataaagg agttttatat gcaaaaattt attcaggctt atattgaaaa gctagatgtg
    25141 acaaccatta tcgagaatat tctaaccaag gtcatttctc ttttactgct tttgattgta
    25201 ttttatattg ctaaaaaaat gcttcatacc atggtgcaga gaattgtcaa accttctcta
    25261 aaaatgtctc gtcatgatgt cggacgccaa aaaaccatct cacgtttact agaaaatgtg
    25321 tttaattata cgctatattt ctttttactc tactgcattt tgtcgatttt aggtttgcca
    25381 gtttctagtt tgctggctgg agctggtatt gctggggtag cgattggtat gggagcccaa
    25441 ggctttctgt ctgatgtcat caatggcttt ttcatcctct ttgaacgtca actggatgtg
    25501 ggagatgagg tcgttctgac aaatggaccg attactgtat cgggtaaggt tgtcagtgtg
    25561 ggaattcgta cgacacagct tcgtagcgag gagcaagccc ttcactttgt ccctaaccga
    25621 aatatcacag ttgttagcaa tttctcacgc acagactaga cctgttattt taagtaattt
    25681 gtggtacaat agagggagtt taataaggag aaaagatggt tttagaaaag cagttgggcc
    25741 atggttgtac ctggatagac ctagacctag gaaagttgaa taaactagaa gacctttctg
    25801 aaatttacgg tttggacaag gaaaccattg aatacgcact ggatagaaac gagcgcgccc
    25861 acatggacta ccaccgtgaa agtgggacgg ttacctttat ctataatgtc ctagacgtaa
    25921 aaaaggacaa ggcctactat gagacttttc ccatgacctt tatcgtcgag catcgtcgcc
    25981 tgattaccat tagtaatacc aagaacgcct atgtcattga acagatgact cgttatctgg
    26041 agagccatga cacgctttcg atttataagt ttctctttgc cagtctggaa atcatcagca
    26101 atgcctacta tcctgtcatt gagcagatgg acaagagtag ggatgaggtc aatgacctct
    26161 tgcgccagcg aactaccaag aaaaacctct ttgccctgtc tgatttggag actggtatgg
    26221 tttatctgac ggcagctgcc aaacaaaatc ggattttgtt agagcatatt caaggtcatg
    26281 ccttgtatcg tagttttgat gagattgaga gagaacagtt tgatgatgcc atgattgagg
    26341 ctcatcagct ggtatccatg acagacctaa tctctcagat tttacagcag ctttcagcct
    26401 cttacaacaa tattctaaac aataatctga atgacaattt gacaaccttg actatcattt
    26461 cagtcttgct agctgttttg gcagtcgtga caggcttttt cggaatgaat gttcccttac
    26521 ctttaacaga tgagccccat gcttggctct atatcagttt ggctagtgca ggtttgtgga
    26581 ttgttttatc cttgttacta aggaaaattg cgaaaaaaag ttaagaaaag gagccagaat
    26641 ggcgattgaa aattatatac cagattttgc tgtggaagca gtctatgatc tgacagtccc
    26701 aagcctgcag gcgcagggaa tcaaggctgt tttggtcgat ttggataata ccctcattgc
    26761 ttggaacaac cctgatggaa cgccagagat gaagcaatgg ctacatgacc ttcgggacgc
    26821 gggtattggc attatcgtag tgtcaaataa caccaaaaaa cgcgttcaac gagcagttga
    26881 gaaatttggg attgattacg tttactgggc cttgaagccc ttcacatttg gtattgaccg
    26941 tgccatgaag gaattccact atgacaaaaa ggaagtggtc atggttggcg accagctcat
    27001 gacagatata cgagcagccc accgtgcagg gattcggtca attttagtca aacccttggt
    27061 ccaacatgac tcaatcaaaa cgcagattaa ccgaactcgt gagcgtcgtg ttatgagaaa
    27121 aatcactgaa aagtacggac cgattacata taaaaaagga atttaactat ggaagaaatt
    27181 ctctgtattg gttgtggagc aaccattcag acgacagata aagctggtct tggttttacc
    27241 ccccagtcgg cacttgaaaa aggtttggag actggcgaag tctattgcca acgctgtttc
    27301 cgtctccgcc actacaatga aatcacagat gtccagttga cgaacgatga tttcctcaag
    27361 ctcttgcacg aggtgggaga cagtgatgcc ttagtggtca atgtcattga tatctttgat
    27421 tttaatggat ctgtcatccc aggtttacca cgtttcgtct cgggcaatga tgtcctcttg
    27481 gtaggaaata aaaaagatat ccttcctaag tcagttaagt ctggtaagat tagccagtgg
    27541 ctcatgaaac gtgcccatga agaaggtctt cgtccagtcg atgtggtcct aacttcagca
    27601 caaaataaac atgccattaa ggaagtcatt gataagattg aacactaccg taagggccgc
    27661 gatgtctatg tggtcggtgt gaccaacgtt ggaaaatcaa ctctaatcaa tgctattatc
    27721 caagaaatca cgggtgatca gaatgtcatc actacttcac gcttcccagg gacaaccttg
    27781 gacaaaatag agattccgct tgacgacgga tcttatattt acgatacgcc gggaattatc
    27841 caccgccacc agatggctca ctacttgacg gccaaaaacc tcaagtatgt cagtcctaaa
    27901 aaggaaatca agcctaagac ctatcagctt aatcctgagc aaaccctatt tttaggtggt
    27961 ttgggacgct ttgactttat agcaggagaa aagcaaggat ttactgcttt ctttgataat
    28021 gaactcaaac tccatcgtag caagcttgaa ggagctagtg ctttctacga taagcacctg
    28081 ggaactcttc tgacaccacc aaatagcaag gaaaaagaag atttcccaag gctagtccag
    28141 catgtcttta ccattaaaga taagacagac ctagtcattt caggcctagg ctggattcgt
    28201 gtaacaggca cagcaaaagt cgccgtctgg gcaccagaag gcgtcgccgt cgccacacga
    28261 aaagcaatta tttaaaacaa aatcgctgag cgaacaaagt gagctcatat agcgatagtt
    28321 attgccgtgg tgtgattgcc aatctgtaga ttggcaaggg gcacaattga gaccaaggct
    28381 caattgtgag gtcaggaaat ccatttttca aagatgaaat ctttggaaaa ttagttccga
    28441 ccgtccctca ccacctaaaa taactatcaa aaaacaagga agaaaattat gtcattagca
    28501 tcaaaacaac gtgccttcct caacagccag gcacacaccc tcaaacctat catccaaatc
    28561 gggaaaaatg gactcaacga ccaaatcaaa accagcgtcc gtcaagctct tgatgcgcgt
    28621 gaattaatca aggttactct cttacaaaac acagatgaaa acatccacga agtagctgaa
    28681 attttggaag aagaaatcgg tgtggataca gtccaaaaaa taggacgcat cttgattttg
    28741 tttaagcaat ctagcaagaa agaaaatcgc aagatttcta agaaagtcaa agaaatctaa
    28801 gaccgaaact ccaaataact gtttttatag agaaataaag gggactagcc tatgacaatc
    28861 gaactattga ctccctttac caaggtagag ttggagccag aaatcaagga gaaaaaacgc
    28921 aaacaagttg ggattttagg ggggaatttt aaccctgttc acaatgccca tctcattgtt
    28981 gcggatcaag tacggcaaca gttgggactg gatcaagttc ttctcatgcc tgaataccaa
    29041 cctcctcacg ttgataaaaa ggaaaccatc cctgaacacc atcgtctcaa gatgcttgag
    29101 ttggcaattg agggaattga cggcctagtc attgaaacca ttgagttgga gcgcaagggt
    29161 atttcctaca cctacgacac catgaagatt ttgacagaga agaatccaga tacggattat
    29221 tactttatca tcggtgccga catggttgac tatctgccta agtggtaccg aattgatgaa
    29281 ctggttgaca tggttcagtt tgtgggggtt cagcgtccac gctacaaggt agggacttcc
    29341 tatccagtta tctgggtgga cgtaccgctc atggatatct cgtccagcat ggtgcgggac
    29401 ttccttgccc aaggtcggaa acccaacttt ctcctacctc agccagtgct agactacatc
    29461 gagaaggagg ggctctactg atgtcctatc aagactatat caactgctcc cgtgaggcct
    29521 tgttggaaaa aatggcagag tttctacctg agaaacgtct aacccattgt ctaggtgtgg
    29581 agcgtgccgc tatggaatta gctcagcgct ttggtgtaga tgttgagaaa gcaagtctag
    29641 caggtcttct tcatgactat gctaaaaagt tgtcaaatca ggaatttcta gccttgattg
    29701 accgttacca gctagaccct gacctcaaaa actggggcaa taatgtctgg catggtatgg
    29761 tcggaattta caaaattcag gaagatttgg atttgcatga ttcagaaatc ctgcgagcca
    29821 ttgaagtcca tacagtcggt gctggtcaaa tgacagacct agataaggtc atctacgtcg
    29881 cagactatat cgagcacaat cgagcctttc ctggagtaga tgtggcgcgt gagattgcaa
    29941 atctatcgct caataaggcg gtggcctacg aaacagctcg taccgtggag tatctagctc
    30001 atcagggatt ccccatctat ccccaaaccc ttgaaaccta taacgccttt gtgcactatt
    30061 tgaaagagaa ctaaatgaag acggctttta taatcattga tgttcagaat attttagtag
    30121 aaactgggtt ccagacaaaa agtctattgg gaaaaatttc ttatttacaa aaccaggcta
    30181 gaagcaagaa tattgaaatt atctatgttc aacatattga gaactctgaa gctcaaacat
    30241 cagaagattg gcagttatct gcgcttttaa atcgaaaacc tgctgaaaag gtctttcaga
    30301 agaagtataa cagtattttc aaagaaactg gcttaaaaga atacttggat aagcagggga
    30361 ttgaaaaatt agttttatgt ggtatgcaga cagaatattg tgtggatacc tctgtcaagg
    30421 ttgcctttga atatggctat cagcttattg ttccagaagg tgctgtcaca acctttgatg
    30481 gggatgacat tccagcagaa acgattaatg aattttatga ggacatttgg gaggagcgct
    30541 ttgcagatgt tctagattac aaacatattt tctaaaaaga ggattaaatg aacgaaaaag
    30601 aattactaga actagtcgtg aaagcggctg atgagaaacg tgcggaggat atcctcgcac
    30661 ttgatgtaca agatttgact agtgtgacgg actactttgt catcactagc tcaatgaata
    30721 gccgtcagtt ggacgctatc gcagctaata tccgtgaaaa agtagctcaa gcaggcttta
    30781 aaggtagcca tgtcgaaggc gatgcagctg gaggctgggt cttactggac cttggtgctg
    30841 ttgtcgtgca tatcttttca gaagaaatgc gtgcccatta taatctagag aagctatggc
    30901 atgaggcgaa ttcagtagat atttcagaag ctcttgctta gaagatgaac tcagttgtag
    30961 tcaactgggt ttctttgctt attttagaaa aattgataga aaggtaaagg gcctcgtgtt
    31021 tgtttcattt cgaaactgaa cacgagctaa aagctgagat aaaaagtaac tttctttgta
    31081 tctgacgata gggaagaaag tttcctatgt actctttgct tttaaggctc tttgtatctt
    31141 agaaaggatt tgaacccgtc ctgtaattct cggaaaatag atagccttcc ttggagctcg
    31201 gctccatcgt aaggctccta attttctatc gaattacggg cggacttggt atcatgtttt
    31261 atggcgactt atgaaacctt tgcggcggtc tatgatgcgg tcatggacga tagtttatac
    31321 gacaaatgga cgaatttttc tctgcgtcat ttgcctaaga ccaaggagag aaagaaactc
    31381 ttggaattgg cttgtgggac aggaattcaa tcagtgcgct tttctcaggc tggttttgat
    31441 gtgactggac ttgacttgag tgcggatatg ttgaagattg cggagaagag agcaacttca
    31501 gccaagcaaa agattgcttt tatagaaggt aatatgctga atttgtccaa ggcagggaaa
    31561 tacgattttg tcacgtgtta ttcggactct atctgctata tgcaggatga ggtggaagta
    31621 ggggacgtct ttaaggacgt gtacaatgcg ctgaatgaag agggagtttt catctttgac
    31681 gtgcactcga cctaccagac agatgaagta ttccctggct attcctacca tgaaaatgcg
    31741 gaagattttg ccatgctttg ggatacatat gaggacgaag ttcctcactc catcgtgcat
    31801 gagctgactt tctttatcaa ggaggctgac ggttccttta gtcgccacga tgaagtacat
    31861 gaggagagga cctatgagat cttgacctat gatatcttgc tggaacaggc tggatttaag
    31921 tccttcaaac tctatgcaga ctttgaggat aaggagccaa cagaaactag cacccgttgg
    31981 ttttttgtgg cgcagaagta ggagattagc atgaccataa caggtattat cgcggagttc
    32041 aatccttttc ataatggcca taaatacctg ctggatcagg cagagggact gaaaattgtt
    32101 gccatgtcag ggaatttcat gcagcgtgga gagcctgcta ttgtggataa gtggacaagg
    32161 gcccagatgg cgctggaaaa tggcgcagac ttggtagtgg aattgccctt tttagtcagt
    32221 gttcaggcag ctgatttctt tggtcaagga gctgtggata tcttggatcg cttgggcatt
    32281 gatagtctag tttttgggac agaggaagtt ctggattacc agaaaatcgc tgacttatac
    32341 acagagaaag gtgctgagat ggagaaattt gtgaaaaatc tgcctgattc cctttcctat
    32401 ccccagaaaa cccaagctat gtggaaggaa tttgctggtc ttgatttttc aggtaatacc
    32461 cccaatcatg ttcttgctct ggcctatgcc aaggcggttg caggacgaaa tatcaagctg
    32521 catccgattc agcgtcaggg ggcaggttac cattctgtga ataaagatgt ggactttgcc
    32581 tcggcgacag ccctccgtca acatcagaag gaccaagatt tcttagaacg ttttatgcct
    32641 tctgttgccc tatttgagca ggccagtaag gtgatctggg aagactattt tcccttgctc
    32701 cgctatcaaa tcttgtcaaa tcccgaccta accaccatct atcaggtcaa tcaagaaatg
    32761 gctgtgcgca ttaaagaagc tattaaaaca gcccagtctg tggaagaatt ggtcgagtta
    32821 gtgactacca aacgttacac caaggcgcgt gtcagacgcc tattgaccta tattttggta
    32881 caggctagag aaaatgtctt gccagaagcc attcatgtcc ttggatttac tgaaaaagtc
    32941 aggcaacatc tcaagtctct gaaaggacag gtcaatctag tcagccgaat tggcaaagaa
    33001 ccttgggatg ctatgaccca aaaagcagac cagatttacc aactagggaa gccaagtata
    33061 gcagagcaaa attttggaag agtgccgatt agaatagaaa caaactaagt ctaccgaaag
    33121 gtaggctttt tgagattttt tacgaataat actcttcgaa aatcaaattc aaatcgcgtc
    33181 aacgtcgcct tgccgtactc aagtacagcc tgcggctagt ttcctagttt gctctttgat
    33241 tttcattgag tataaataga tagaaaagaa aatcttgtac aagttcctga caatttcttt
    33301 cttttcgtgt ataatagtgg aaaagagcta aaacgaggta tggttatgga agcagtcctt
    33361 tactcaacat tccgaaatca tttaaaggac tacatgaaga aggtaaatga tgaatttgag
    33421 cctttgacgg tggtcaataa aaatccagat gaggacattg tagttctttc aaagagcgaa
    33481 tgggacagta ttcaggaaac cctgagaatc gcccaaaata aggagttatc agacaaggtt
    33541 ttgcgaggaa tggctcaagt ccgtgctgga agcactcagg tccatgtgat tgaggagtga
    33601 tgaatgctac tcaagtttac agaagatgcc tgggcagatt attgctactg acaaaaccaa
    33661 gataaaaaaa cactaaaaag aatcaataaa ttaatcaagg atattcaacg cgatccattt
    33721 acaggaatag gaaaaccaga gccactcaaa tatgattacc aaggagcctg gtcacggcgt
    33781 attgatgcag aaaatcgctt gatttatatg atggatggag atagcgtggc tttcttgtcc
    33841 tttaaagatc attactaagt ctactgcaaa gtaggctttt tctcaagtct ctactaaact
    33901 actatttcta aacaacaagt ttattttggt aaatgtgttc caaattcccc aaaacatatt
    33961 ctattttagg tttgtgaaaa tcactttttt atggtagaat gtaaaagaat gtatgtcatt
    34021 cggaataaca ataaaatgag gtaaaaacat ggaaatcatg tcgcttgcga ttgctgtttt
    34081 tgccgtcatc attggtttag tcattggata tgtcagcatc tcagctaaga tgaaatcatc
    34141 tcaggaagct gcagagttga tgcttttaaa tgctgaacaa gaagcaacta atttacgtgg
    34201 acaagctgag cgtgaagcgg atttacttgt taatgaagcc aaacgtgaaa gcaagtctct
    34261 taaaaaagaa gcactattgg aggccaaaga agaagccaga aaataccgtg aagaagtgga
    34321 cgctgaattc aaatcagaac gtcaagaact caaacaaatc gaaagtcgtt tgacagagag
    34381 agctactagc cttgaccgta aggacgacaa tttgacgagt aaagaacaaa cacttgaaca
    34441 aaaagaacaa agtatttctg atagagcgaa aaaccttgat gcgcgtgaag agcaattaga
    34501 ggaagtcgaa agacaaaaag aagcagaact agagcgtatt ggtgctctgt ctcaggcaga
    34561 agcacgagat attatcttgg ctcagacaga ggaaaacttg accagggaga ttgccagtcg
    34621 cattcgcgaa gctgagcaag aggtcaagga acgttctgac aaaatggcca aggacatctt
    34681 ggttcaagct atgcaacgta tcgctggtga atatgtagcg gagtcaacaa actcaacagt
    34741 tcatctgcca gacgatacta tgaagggacg cattattggt cgtgaaggtc gtaacattcg
    34801 tacctttgaa agtttgacag gggtcgatgt gattatcgac gatacaccag aagtggtgac
    34861 cttgtcagga tttgatccga ttcgtcgtga gattgcccgt atgactatgg aaatgttgct
    34921 caaagatggt cgtatacatc cagctcgtat cgaagagttg gttgagaaaa accgtcaaga
    34981 gattgacaat aagattcgtg aatacggtga ggctgctgcc tatgaaattg gtgcgccaaa
    35041 ccttcatcca gacttgatga agattatggg acgtttgcag ttccgtactt catatggaca
    35101 aaatgttttg cgccattcga ttgaggttgc taagttggct ggtatcatgg cgagcgaact
    35161 tggtgaaaat gcggctcttg cccgtcgtgc tggattcctt cacgatatcg ggaaagccat
    35221 tgaccatgag gttgaaggta gccacgttga aatcggtatg gaattggccc gtaagtacaa
    35281 ggaaccccca gttgtggtga atacgattgc tagtcaccac ggagatgttg aagctgagag
    35341 cgtgatagca gttatcgtcg ctgcagcaga tgccttgagc gcagcccgtc caggtgctcg
    35401 tagtgagtct cttgaaagct acatcaagcg tctccatgat ttggaagaaa ttgctaacgg
    35461 ctttgaagga gtgcaaacta gctttgccct tcaagcagga cgtgaaattc gtatcatggt
    35521 caatccagga aaaatcaagg acgacaaagt cacaatcttg gctcacaaag ttcgtaagaa
    35581 aattgaaaac aatctcgatt atccaggaaa tatcaaggta accgtgattc gcgagcttcg
    35641 tgcagtagat tatgctaaat aaataagaaa gagcagttga aaaattactg cttttttgtt
    35701 acactagata gaaagactgt aaaggataat ctggcttttt gccattattc tagagaaatg
    35761 ttatttcaca gattgactaa aaggagacac aatggcagac cgaggcttac taatcgtttt
    35821 ttctggtcct tcaggggttg gaaaaggaac ggttagaaga gagatttttg agagttctga
    35881 aaaccaattt caatattctg tatcgatgac gacacgcgca caacgtcctg gagaagtgga
    35941 cggtgttgac tatttcttcc gtactcgtga agaatttgaa gagctgattc gtcaaggaca
    36001 gatgttggaa tacgcagaat atgtcggcaa ctactatgga actcctctga cctatgtcaa
    36061 tgaaaccttg gacaagggaa tcgatgtttt ccttgaaatt gaagttcagg gtgctcttca
    36121 ggtcaagaaa aaggttccag atgctgtctt tatcttcctg acaccaccag atttggatga
    36181 attgcaagat cgcttggtag gtcgtggaac agatagtgca gaagtgattg cccaacgaat
    36241 cgaaaaggcc aaggaagaaa ttgccctcat gcgtgagtat gattatgcga ttgtcaacga
    36301 tcaggtgccc ctagctgctg aacgtgtcaa atgtgtgatt gaagcagaac acttctgtgt
    36361 ggatcgtgtc attggtcact atcaggagat gttaccaaaa tctccaacta cccgataaaa
    36421 tttagaaaat aggtacaagc aaatgatgtt aaaaccctct attgatacct tgctcgacaa
    36481 ggttccttca aaatattcac tcgtaatctt ggaagcaaaa cgtgcccacg aattggaagc
    36541 aggtgcccca gcaactcaag gtttcaagtc tgaaaaatca actcttcgcg ctttagaaga
    36601 aatcgaatca ggaaacgtta caattcaccc agatccagaa ggaaaacgtg aagcagtgcg
    36661 tcgccgtatc gaagaagaaa aacgccgcaa agaagaagaa gaaaagaaaa tcaaagagca
    36721 aattgctaaa gaaaaagaag atggtgaaaa aatttaaggt tggggggact caatcttatt
    36781 ttttctattg caagaatgta ctgacaagga ggaggtgaga agatggccct agctaagatt
    36841 atcgtagatg tgcccttgat gcagacggac cagccctata gttacaggat tccggaggaa
    36901 tttgagggaa tgctggaagt tgggatgcgg gttcatgtgc cttttggtaa gggtaatcgc
    36961 ctgattcaag ggattgttct tggtttgaag tcccaatcag atggagaaga gatggagcaa
    37021 gatttaaaag atattgcaga ggtgctggat ttttctcctg ttctcacgcc agaacaactc
    37081 tggctggctg aggagttacg caaatctgtc ttctcctaca aaatctctat cctcaaggcc
    37141 atgcttccag ggtttctgaa ttctagctat gacaagattc tctatcctct ggaaggcttg
    37201 agtcaggaag aacgagtacg cctgtttggt tcagaagatt cgctagcctt ttcttcacta
    37261 gaccttgcca agcaagctga aatgatgcgt ttgactagaa aaggcctgct tggtctggaa
    37321 tatcaggcag tcgatcaaaa gaaggtcaag acccagtctt ggtatgaggt tgaccatgcc
    37381 caattagaag gtgttgagat ttctacacgt gccaagaaaa agttggaact gagagattat
    37441 ctgctgtctc atccagagag cgcttccttg gctagcttgt tagagtccta ctcgcgagag
    37501 caagtcaact tctttgtgga tcaaggtact gttaccatag tccaaaagga agttcaacgc
    37561 tcggctgctt attttgaagg aattgaagca agtagacctt tggagttaaa tccagaacaa
    37621 agacaggcgc gtgatgcggt tgtgagttct attggcagtt ctcaacctcc ctttctactt
    37681 caagggatta caggaagtgg gaagaccgag gtttacttgc agattatcca aggtgccctg
    37741 gataagggta agacagctat tttgctggta cctgagattt ccttgactcc tcagatgacc
    37801 gagcgtttta tagcacgttt tggggacaag gtagccattc ttcactcagg attatccaat
    37861 ggcgaaaagt atgatgaatg gcgcaaggtg gagcgtggcg atgcccaagt tgttgttggt
    37921 gccagatctg ctatctttgc tcctctgaaa aatctgggtg tcatgattat tgatgaagag
    37981 catgaagcgg cttataagca ggacagcaat ccccgttacc atgccagaga ggtagctatt
    38041 ttacgggctc agtataatca agcaactctg gtacttggat ctgcaacgcc gagcttagag
    38101 agccgggcac gtgctggcaa aggcgtctat caacacttac gtctaaccca acgtgccaat
    38161 cctttggcta caatccctga ggttcaagtg attgactttc gagactatat cggacaaaat
    38221 gagacgtcaa actttacgcc tcctttgcta gaggctatcc aagaccgtct ggttaaaaaa
    38281 gagcaggtgg ttctcatgct caatcgccgt ggttattcta gctttgtcat gtgtcgggag
    38341 tgtgggacgg tggatacttg tcccaactgc gatatttctc tgaccttgca tatggatacc
    38401 aagactatga actgccatta ttgtggtttc tcgaaggata ttcctcaggt ctgtcctaac
    38461 tgtaagagcc gcagtattcg ttactatggg acgggaactc agaaggctta tgatgagcta
    38521 gcagaactct ttccccaagc tcgcattttg aggatggatg tggatacgac tcgaaagaaa
    38581 ggcagtcacc aagccttgct tgaccagttt gggcgagggg aagcggatat tttacttggt
    38641 actcagatga ttgcaaaggg tttggatttt cccaatgtta ccctagtcgg agttctaaat
    38701 gcggatacgg ccttgaatct gcctgatttc cgttcttctg agagaacctt ccagctcttg
    38761 actcaggtgg caggtcgagc aggtcgggct gaaaaagcag gtcaagtctt gatacagtcc
    38821 tacaattcgc agcactatgc tattcgattt gccaaggatc aagattacga aggcttttat
    38881 gcctatgaaa tgggaatcag acgacaactc ggctatccac cttactattt cacgattggt
    38941 attacccttt ctcacaagaa agaagaagag gttttcaaac gtgcctatga agtcatgaac
    39001 attttgcggt caggcttgtc agagaccagt cccattctgg ggccaacgcc aaaaccgatt
    39061 gcccgtaccc acaacctcta tcattaccag attttaatta aataccgttt agaagatgag
    39121 ctggggccga ccctcaacca ggtcttggcc ttgactcaag aacgggaaaa tagtgagctc
    39181 cgtctcagca ttgaccatga gccgcagcaa tttttataag aaggagaaga tatgacaaaa
    39241 ctaatcttta tggggacccc cgacttttca gcaacagtct taaaaggact tttgacagat
    39301 gaccgttacg aaattctagc cgttgtgacc cagccagacc gtgctgttgg tcgtaaaaaa
    39361 gttatccaag aaaccccagt caagcaggct gccaaggaag caggactatc tatctaccaa
    39421 cctgaaaaat tatctggaag tccagagatg gaagaactta tgaagctagg agcagatgga
    39481 attgtgactg ctgcttttgg gcagtttctc ccaagcaaac tccttgatag catggacttt
    39541 gctgtcaacg ttcatgcctc cctccttcct agacaccgtg gtggtgcgcc tatccattat
    39601 gccttgattc aaggggatga ggaagctggt gtgaccatca tggaaatggt taaggaaatg
    39661 gatgcaggag atatgatttc tcgtcgcagc attccgatca cagatgagga caatgttggc
    39721 accttgtttg aaaaattggc gctagttggt cgtgatttgc ttttggacac tctgcctgcc
    39781 tatattgctg gtgatatcaa acctgaaccg caggatacga gtcaggttac cttctctcca
    39841 aatataaagc cagaggaaga aaaactggac tggaacaaaa ccaatcgtca actctttaac
    39901 caaattcgtg gaatgaaccc ctggcctgtt gcccatactt tccttaaggg cgaccgcttt
    39961 aagatttatg aagccctacc agtagaaggt cagggaaatc caggtgagat tctctctatc
    40021 ggcaagaaag aattgattgt cgcaacggct gaaggggctc tatccctcaa acaagtgcag
    40081 ccagctggta agcctaagat ggacattgct tccttcctca atggagttgg acgtacattg
    40141 actgtaggag aacgatttgg tgactaaagt agaaacggct agaagtttag ctctagcagt
    40201 gctagaggat gtttttgtga atcaagcata ttcaaatatt gccttaaata aacacctcaa
    40261 ggggagtcag ctctctgcag cagacaaggg cttagtgacc gagctagtct atggaacggt
    40321 agcccgtaaa ctgactctgg aatggtacct atcccacttt atcgaagaca gagaccagtt
    40381 agacagctgg ctctatgtcc ttcttctcat gagtgcctac caactccgct atttggacaa
    40441 gattccagat catgctgtgg tcaatgaagc agtggaattg gccaaactcc gtaaaaaagg
    40501 cagtgaaaaa ttggtcaacg ctgtccttcg ccgtatcttg cgtgaaggct ggccagatat
    40561 tgctagcatc aagcgaaaaa acaagcgtga ctccattgcc tattctctcc cagtttggct
    40621 agttgccaaa ctcaaggaag aatacggaga ggagagagca aaagccatct ttgaaagcct
    40681 gttagtgcga aacaaagcca gtattcgtgt aacagaccta agtcgaaaag aggaaatcca
    40741 agccttgttg gaggcgaata attccctttt agcaaccact ggtctggtta aggagcaggg
    40801 gcattttgca gggcataatt tgtttgcgga tggagccatt accatccaag acgagtccag
    40861 tcagttggtt gctccgacgc ttgatttaca aggtgatgag cgagtgcttg atgcctgtgc
    40921 ggctccaggt gggaaaacag cccatatagc ctcttatctt acgacaggtc aggttactgc
    40981 tctggacttg tacgaccaca agttggattt aattcaagaa aatgcccaac gtctgggagt
    41041 tgaagatcgg gttcaaactc aaaaattgga tgccagaaag gtgcatgagt tttttgacca
    41101 ggattccttt gataagattt tggtggatgc tccttgttca ggaatcggtc ttttgcgccg
    41161 aaaaccagat atcaaataca ataaagaaac ggcagatttc gcgtccttgc aggaaattca
    41221 gttagaaata ttaggtagtg tttgtcaaat actaggcaaa ggtggtataa taacttatag
    41281 cacctgcact atcgtctcag aggagaattt tcaagtcgtt aaggcctttt tagagagtca
    41341 tcccgagttc gagcaggtaa aactagaaca tgaatgtaag gatatcatga aagacggctg
    41401 tatcctcatt acacctgaat tgtatggaag tgatggattc ttcatcagtc aatttcgcaa
    41461 gatatcggat taggaaggaa ctgacacatg gaaatttcat tattaacaga tgttggtcag
    41521 aaacgaacaa ataaccaaga ctatgtcaac cactatgtca atagagctgg acgtaccatg
    41581 attattttag ctgatgggat gggaggtcat cgtgcaggga atatcgctag tgaaatggcg
    41641 gtcacagacc tgggtgtagc ttgggttgat acccagatcg atacagtcaa tgaagtgcgt
    41701 gaatggttcg cccattacct agaaattgaa aatcaaaaga ttcaccagct tggtcaggat
    41761 gaagcttaca gaggcatggg aactactttg gaagtccttg ctattattga taatcaggct
    41821 atctatgctc atattggtga ttcgcgtatc ggcttgattc gtggagaaga ataccatcag
    41881 ttgacgagcg atcattcctt ggttaatgaa ttgctcaagg ctggtcaatt gacaccagaa
    41941 gaggcagaag ctcatccgca aaaaaatatt atcacccagt ctattgggca aaaagatgaa
    42001 attcagcctg attttgggac agttatcctt gagtcaggtg actatctctt gctcaatagt
    42061 gacggcttga ccaacatgat ttcaggcagt gagattcgtg atattgtaac cagtgatatt
    42121 cctttagcag ataaaacgga gacacttgtt cgttttgcta acaatgcagg aggtttagac
    42181 aacattacgg ttgcccttgt ttttatgaac gaggaggatg cagaatgatc caaatcggca
    42241 agatttttgc cggacgctat cggattgtca aacagattgg tcgaggaggc atggcggatg
    42301 tctacctagc caaagactta atcttagatg gggaagaagt ggcagtgaag gttctgagga
    42361 ccaactacca gacggacccg atagctgtag ctcgttttca gcgtgaagcg agagctatgg
    42421 cagatctaga ccatcctcat atcgttcgga taacagatat tggcgaggaa gacggtcaac
    42481 agtacctagc tatggagtat gtggctggac tggacctcaa acgctatatc aaggaacatt
    42541 atcctctttc taatgaagaa gcagtccgta tcatgggaca aattctcttg gctatgcgct
    42601 tggcccatac tcgaggaatt gttcacaggg acttgaaacc tcaaaatatc cttttgacac
    42661 cagatgggac tgccaaggtc acagactttg ggattgctgt agcctttgca gagacaagtc
    42721 tgacccagac taactcgatg ttgggctcag ttcattactt gtcaccagag caggcgcgtg
    42781 gttcgaaggc gactgtgcag agtgatatct atgccatggg gattattttc tatgagatgt
    42841 tgacaggcca tatcccttat gacggggata gcgcggtgac cattgccctc cagcatttcc
    42901 agaaacccct gccgtccgtt attgcagaaa atccatctgt acctcaggct ttagaaaatg
    42961 ttattatcaa ggcaactgct aaaaagttga ccaatcgcta ccgctcggtt tcagagatgt
    43021 atgtggactt gtctagtagc ttgtcctaca atcgtagaaa tgaaagtaag ttaatctttg
    43081 atgaaacgag caaggcagat accaagacct tgccgaaggt ttctcagagt accttgacat
    43141 ctattcctaa ggttcaagcg cagacagaac acaaatcaat caaaaaccca agccaggctg
    43201 tgacagagga aacttaccaa ccacaagcac cgaaaaaaca tagatttaag atgcgttacc
    43261 tgattttgtt ggccagcctt gtattggtgg cagcttctct tatttggata ctatccagaa
    43321 ctcctgcaac cattgccatt ccagatgtgg caggtcagac agttgcagag gccaaggcaa
    43381 cgctcaaaaa agccaatttt gagattggtg aggagaagac agaggctagt gaaaaggtgg
    43441 aagaagggcg gattatccgt acagatcctg gcgctggaac tggtcgaaaa gaaggaacga
    43501 aaatcaatct ggttgtctca tcaggcaaac aatccttcca aattagtaat tatgtcggcc
    43561 ggaaatcttc tgatgttatc gcggaattaa aagagaaaaa agttccagat aatttgatta
    43621 aaattgagga agaagagtcg aatgagagtg aggctggaac ggtcctgaag caaagtctac
    43681 cagaaggtac gacctatgac ttgagcaagg caactcaaat tgttttgaca gtagctaaaa
    43741 aagctacgac gattcaatta gggaactata ttggacggaa ctctacagaa gtaatctcag
    43801 aactcaagca gaagaaggtt cctgagaatt tgattaagat agaggaagaa gagtccagcg
    43861 aaagcgaacc aggaacgatt atgaaacaaa gtccaggtgc cggaacgact tatgatgtga
    43921 gtaaacctac tcaaattgtc ttgacagtag ctaaaaaagt tacaagtgtt gccatgccga
    43981 gttacattgg ttccagcttg gagtttacta agaacaattt gattcaaatt gttgggatta
    44041 aggaagctaa tatagaagtt gtagaagtga cgacagcgcc tgcaggtagt gtagaaggca
    44101 tggttgttga acaaagtcct agagcaggtg aaaaggtaga cctcaataag actagagtca
    44161 agatttcaat ctacaaacct aaaacaactt cagctactcc ttaaaagcag atggataatc
    44221 aaaatgagta atcaatcttt gtcatgattt catggcaaag attttttttg agtccggatt
    44281 tgtgatagaa tagagggagt tgataaaagg aggaaagcat ggaagaatca aaagaattaa
    44341 atgccgtcat tgatgtgatt atgctagcgg ggactattct ccttaaaagt ggctcagaaa
    44401 tccatcgtgt agaagatacc atgattcgaa tcgcgcattc gcaggggatt gtggattgca
    44461 atgtccttgc catgcctgcc gctatctttt tctctattga aaataccaat atttcgcgca
    44521 tgaagcgcgt gacctcctct tcttataaca tcgaaaaagt ctgcgatgtg aaccagattt
    44581 ctcgtcagct agttgggggg cagattgact tagaaacagc ctttaagcaa ttgacggcct
    44641 tgcaagctca accccttccc tatactaagt tgcaggtaac tctggctgcg acctttagtg
    44701 ctcctttctt ttcagttatg tttagcggaa atatctacga cgcacttggg gcaggagtgg
    44761 cgaccttatt tggttttgcc ttttccctct atgtggaaaa atttatccga attccctttg
    44821 tgacagcctt tgctggagcc tttgtctttg ggataattgc ccagttttgg gctcgctaca
    44881 caggttttcc ttcaacggca gatttgatta tagctggtgc ggtcatgccg tttgtaccag
    44941 gtattgcctt gaccaatgcg gtccgtgata ttatgaccaa ccacataaac tctggtatga
    45001 gtaagatgtt tgaatccctg ctcattaccc tcgctttagg ggcaggaact tctgtcgcct
    45061 tggtattgat gaactaatat gacactaaca acctttttat tacaagcagt agcaagtttt
    45121 cttgccatta tcactttttt aatcgtactc aatgtgcaac ggtctatgct cttacctgga
    45181 gggattttgg gcatgactgt ctggctaatc tatctcttgc tcaaggaacc gaccaatgtc
    45241 attgtagcta ccttcattgc agccattatt ggttcttgtg tcagccagat tttaagtatt
    45301 ctttataaga cacctgctgt ggtctttatc ttggccattt tggcaccgct ggttccaggt
    45361 tatctctcct accgaacaac tgcctttttt gtgacagggg actataataa agcactggca
    45421 agtgcgacct tggttgtcat gttggctttg gtaatctcta ttggaatggc tagcggaaca
    45481 gtgattctca gactgtatca ttatataaaa acacatcgag tatcgtagac tttacagaaa
    45541 taaaagaatt ttctgaaaaa tgagataaat aaattaacaa cgctttctat atgtgcgaga
    45601 ataccgcact tatgaagaaa ttgcggctga ttttggtatc cacgaaagca acttactccg
    45661 tcggagccaa tgggttgaag taactcttgt tcaaaggtgg tgttacgatt tcaagaactc
    45721 ctctcagttc tgaagacacg gtaatgattg atgcgacgga agtaaaaatc aatcgcccta
    45781 aaaaaaacaa ttagcgaatg attctggtaa aaagaaattt cacgctatga aggct
//
LOCUS       DAVBCB010000010        45159 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVBCB010000010 DAVBCB010000000
VERSION     DAVBCB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 45159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45159
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.010"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(56..1066)
                     /locus_tag="VQU28_000536"
     CDS             complement(56..1066)
                     /locus_tag="VQU28_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HET2475944.1"
                     /translation="MSFLSKNGAGILACLLISIVSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIDAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKELSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="VQU28_000537"
     CDS             1215..2384
                     /locus_tag="VQU28_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2475945.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="VQU28_000538"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="VQU28_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HET2475946.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="VQU28_000539"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="VQU28_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HET2475947.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="VQU28_000540"
     CDS             4146..4379
                     /locus_tag="VQU28_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HET2475948.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQSVGDVVKIIQGK"
     gene            4425..4716
                     /locus_tag="VQU28_000541"
                     /pseudo
     CDS             4425..4716
                     /locus_tag="VQU28_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4919..5149
                     /gene="blpU"
                     /locus_tag="VQU28_000542"
     CDS             4919..5149
                     /gene="blpU"
                     /locus_tag="VQU28_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2475949.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAATCWW"
     gene            5864..8017
                     /locus_tag="VQU28_000543"
     CDS             5864..8017
                     /locus_tag="VQU28_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HET2475950.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPYKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTGINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            8030..9379
                     /locus_tag="VQU28_000544"
     CDS             8030..9379
                     /locus_tag="VQU28_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HET2475951.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAMETGASLASQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            9550..10257
                     /locus_tag="VQU28_000545"
     CDS             9550..10257
                     /locus_tag="VQU28_000545"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HET2475952.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIAGDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQELK"
     gene            10459..14184
                     /locus_tag="VQU28_000546"
     CDS             10459..14184
                     /locus_tag="VQU28_000546"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HET2475953.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLAEDLFAPAEKHIFSEQGTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANSTSPTLFYNDANQHVTKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            14277..15719
                     /locus_tag="VQU28_000547"
     CDS             14277..15719
                     /locus_tag="VQU28_000547"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HET2475954.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGAGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            15756..16778
                     /locus_tag="VQU28_000548"
     CDS             15756..16778
                     /locus_tag="VQU28_000548"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HET2475955.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            16775..17320
                     /gene="purN"
                     /locus_tag="VQU28_000549"
     CDS             16775..17320
                     /gene="purN"
                     /locus_tag="VQU28_000549"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HET2475956.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKADYEAALVELLEEHQIALVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            17404..17913
                     /locus_tag="VQU28_000550"
     CDS             17404..17913
                     /locus_tag="VQU28_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2475957.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLIFFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            17938..19485
                     /gene="purH"
                     /locus_tag="VQU28_000551"
     CDS             17938..19485
                     /gene="purH"
                     /locus_tag="VQU28_000551"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HET2475958.1"
                     /translation="MTKRVLISVSDKADIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDGVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYDDAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGENKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            19607..20869
                     /gene="purD"
                     /locus_tag="VQU28_000552"
     CDS             19607..20869
                     /gene="purD"
                     /locus_tag="VQU28_000552"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HET2475959.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVSTAAYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVSETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFFEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGMIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNITWTDKGVTLGVVVA
                     SKGYPLNYSKGVELPVKTDGDIITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            21272..21760
                     /gene="purE"
                     /locus_tag="VQU28_000553"
     CDS             21272..21760
                     /gene="purE"
                     /locus_tag="VQU28_000553"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HET2475960.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            21747..22838
                     /gene="purK"
                     /locus_tag="VQU28_000554"
     CDS             21747..22838
                     /gene="purK"
                     /locus_tag="VQU28_000554"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HET2475961.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            22848..23075
                     /locus_tag="VQU28_000555"
     CDS             22848..23075
                     /locus_tag="VQU28_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HET2475962.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            23138..24436
                     /gene="purB"
                     /locus_tag="VQU28_000556"
     CDS             23138..24436
                     /gene="purB"
                     /locus_tag="VQU28_000556"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HET2475963.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(24491..28429)
                     /gene="strH"
                     /locus_tag="VQU28_000557"
     CDS             complement(24491..28429)
                     /gene="strH"
                     /locus_tag="VQU28_000557"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2475964.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKDE
                     AKAEDDKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTD
                     LHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTD
                     LINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESARTVNLDNEQAV
                     AFTKALIDKYAAYFAKKTEIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYE
                     KFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVAS
                     SKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIP
                     IIGSMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQTLNEVPKDLNRY
                     TAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKR
                     EVEKLAKNKVISIDAGRKYFTLNQLKRIVDKASELGYSDVHLLLGNDGLRFLLDDMTI
                     TANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSP
                     GHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKI
                     FNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYE
                     DKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGG
                     FLKKAIENTGKTPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFA
                     DHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTL
                     VANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAG
                     QENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESG
                     LAPTTEVKPRLDIQKEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADG
                     KEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAPA
                     EESKALPQDPAPVVIEKKLPETGTHDSAGLVVAGLMATLAAYGLTKRKED"
     gene            complement(28744..29460)
                     /locus_tag="VQU28_000558"
     CDS             complement(28744..29460)
                     /locus_tag="VQU28_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2475965.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            29808..31595
                     /locus_tag="VQU28_000559"
     CDS             29808..31595
                     /locus_tag="VQU28_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HET2475966.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            31592..32068
                     /locus_tag="VQU28_000560"
     CDS             31592..32068
                     /locus_tag="VQU28_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2475967.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            32096..33001
                     /locus_tag="VQU28_000561"
     CDS             32096..33001
                     /locus_tag="VQU28_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2475968.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            32988..33803
                     /locus_tag="VQU28_000562"
     CDS             32988..33803
                     /locus_tag="VQU28_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2475969.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            33810..34214
                     /locus_tag="VQU28_000563"
     CDS             33810..34214
                     /locus_tag="VQU28_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2475970.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            34513..35679
                     /locus_tag="VQU28_000564"
     CDS             34513..35679
                     /locus_tag="VQU28_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HET2475971.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVVLGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            35795..36832
                     /locus_tag="VQU28_000565"
     CDS             35795..36832
                     /locus_tag="VQU28_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HET2475972.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGALLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            37040..37894
                     /locus_tag="VQU28_000566"
     CDS             37040..37894
                     /locus_tag="VQU28_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2475973.1"
                     /translation="MTYEYKSHIYLAETALNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREYYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            38044..38301
                     /locus_tag="VQU28_000567"
     CDS             38044..38301
                     /locus_tag="VQU28_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HET2475974.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDRDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(38677..38769)
                     /locus_tag="VQU28_000568"
                     /pseudo
     CDS             complement(38677..38769)
                     /locus_tag="VQU28_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(38805..39101)
                     /locus_tag="VQU28_000569"
     CDS             complement(38805..39101)
                     /locus_tag="VQU28_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2475975.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLISQGLTVTNVEGGMNAWPGQVE"
     gene            complement(39091..39252)
                     /locus_tag="VQU28_000570"
     CDS             complement(39091..39252)
                     /locus_tag="VQU28_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(FAD)-dependent dehydrogenase"
                     /protein_id="HET2475976.1"
                     /translation="MDKKQAYIVSCHSGLRSYIAEPILKQAGFTAQNLDGAYSLYKMV
                     NPEGVEYGN"
     gene            39322..40086
                     /locus_tag="VQU28_000571"
     CDS             39322..40086
                     /locus_tag="VQU28_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HET2475977.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLDEEI
                     DRYAREVGADCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            40699..42078
                     /locus_tag="VQU28_000572"
     CDS             40699..42078
                     /locus_tag="VQU28_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2475978.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            42092..42757
                     /locus_tag="VQU28_000573"
     CDS             42092..42757
                     /locus_tag="VQU28_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HET2475979.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            42871..42966
                     /locus_tag="VQU28_000574"
                     /pseudo
     CDS             42871..42966
                     /locus_tag="VQU28_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            43112..43561
                     /locus_tag="VQU28_000575"
     CDS             43112..43561
                     /locus_tag="VQU28_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2475980.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDAFVTNFVYEKEGQSRKKSMSYESVLPVRQIFD"
     gene            43718..44128
                     /locus_tag="VQU28_000576"
     CDS             43718..44128
                     /locus_tag="VQU28_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HET2475981.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKRIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            44244..>45159
                     /gene="pavB"
                     /locus_tag="VQU28_000577"
     CDS             44244..>45159
                     /gene="pavB"
                     /locus_tag="VQU28_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HET2475982.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT        12581 a         8714 c        10654 g        13210 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttctttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggagaga aagagcgtga tagggataat agccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatcaata acgggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatacg atagaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaattttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcggccttca
     1981 cagcccagtt aatcaagagt caccaatact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaatgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gcctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggcagcagg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct ggactcatgt ctaccttgcc taccgttgat
     3541 ggaaaaggtt ttgacatgct agaccttggt gccaatgcag aaaatacagc ccagcacctc
     3601 catcaatatg cggttctagg ttccttctat gctaaaaatg tccgtggcat tgcgcaacca
     3661 cgcgttggtt tgctcaacaa cggaacagag agtagcaagg gcgacccgct tcgtaaggaa
     3721 acttatgaat tactggcggc tgatgaaagt ttgaacttta tcggaaacgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgtcagatc
     4081 cgtaccatgc tagaaacaga cgtggttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aggaaattga ccaactccaa agtgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aatgagaaat atcggacaag
     4441 ctggtaaaat cttggctgac agtggttatc aagggctcat gaagatatat cctcaagcac
     4501 aaacttccac gtaaatccag caaactcaag ccgctaacag ttgaagataa agcctgtaat
     4561 catgcgctat ctaaggagag aagcaaggtt gagaatatct ttgccaaagt aaaaacgttt
     4621 aaaatgtttt caacaaccta tcgaaatcat cgtaaacgct tcggattacg aatgaatttg
     4681 attgctggta ttatcaatca tgaactagga ttctagtttt gcaggaagtc taatagtaaa
     4741 aaagtgatta gaaaacatct tttttaaaaa tagagatgat tttgaaacaa aaaagctaat
     4801 tcaagacgtt tcgatgccaa ttcaagattt ggatgaaaaa aattaataga tactgttata
     4861 ctaaacttgt caagtttgta acaagacaaa tattaaaaat aaaaaagagg tattcgttat
     4921 gaatacaaaa aagatgtcac aatttgaaat tatggatact gagatgcttg cttgcgttga
     4981 aggtggcgga tgcaattggg gagattttgc caaagcaggt gttggaggag gagcagtacg
     5041 aggtcttcag ctaggaatta aaacaagaac atggcaaggt gcagcaactg gtgctgcggg
     5101 aggagctata cttggaggtg tggcctatgc agcgacatgt tggtggtaat tatggatttt
     5161 aaaagtttta ttattggttt agtagttggt atatttggtc cttatatgga tgatttaatt
     5221 agaaaaaaat ttttaaagtc ttcggagaag aaaacagaaa aatctgttaa aaaataatca
     5281 aaactataaa tgatgaatct gaatcaaaat tattttgcgc atgtaaagag gagtcttata
     5341 gtaacgagtc aaaaaaggag taactatgaa tcgtaattta gaacggtgtt atctattctg
     5401 actaggaata gatcatacca gaggtagctt agaaatagca gggacattag aaattgaagt
     5461 aataaatagg atgtcgtaag tgttactatc aatgatttat ttgtttcaag cttgcctagg
     5521 gtgacagtaa aaaatcaatt tcctttcaat agcatatttt tagtgggcag gactcttgtt
     5581 ctgcctattt ttttatccaa aaagtgcagt tgggagggag ataggctcat ttgggaagga
     5641 agtccagttt ttgtttagtg attggggtaa gatagttgtt atcagatgag tttatactct
     5701 tcgaaaatca aattcaaacc acgtcaacgt cgccttgccg tatatatgtg actgacttcg
     5761 tcagtcctat ctacaacctc aaaacagtgt tttgagcagc ctgcggctag tttcctagtt
     5821 tgctctttga ttttcattga gtattaggga aaaggagatg aatatgaaat ttgggaaacg
     5881 tcactatcgt ccgcaagtgg atcagatgga ctgcggtgta gcttcattag ccatggtttt
     5941 tggctactat ggtagttatt attttttggc tcacttgcga gaattggcta agacgaccat
     6001 ggatgggacg acggctttgg gcttggtcaa ggtggcagag gagattggtt ttgagacgcg
     6061 agccattaag gcggatatga cgctttttga cttgccggat ttgacttttc cttttgttgc
     6121 ccatgtgctt aaggaaggga aattgctcca ctactatgtg gtgactgggc aggataagga
     6181 tagcattcat attgccgatc cagatcccgg ggtgaagttg actaaactgc cacgtgagcg
     6241 ttttgaggaa gaatggacag gagtgactct ttttatggca cctagtccag actataagcc
     6301 ttataaggaa caaaaaaatg gtctgctctc ttttatccct atattagtga agcagcgtgg
     6361 cttgattgcc aatatcgttt tggcaacact cttggtaacc gggattaaca ttgtgggttc
     6421 ttattatctg cagtctatca ttgataccta tgtgccagat cagatgcgtt cgacactagg
     6481 gattatttct attgggctag tcatcgtcta catcctccag caaatcttgt cttacgctca
     6541 ggagtatctc ttgcttgttt tggggcaacg cttgtcgatt gacgtgattt tgtcctatat
     6601 caagcatgtt tttcacctcc ctatgtcctt ctttgcgaca cgcaggacag gggagatcgt
     6661 gtctcgtttt acagatgcta acagtatcat cgatgcgctg gcttcgacca tcctttcgat
     6721 tttcctagat gtgtcaacgg ttgtcattat ttcccttgtt ctattttcac aaaataccaa
     6781 tctctttttc atgactttat tggcgcttcc tatctacaca gtgattatct ttgcctttat
     6841 gaagccgttt gaaaagatga atcgggatac catggaagcc aatgcggttc tgtcttcttc
     6901 tatcattgag gacatcaacg gtattgagac tatcaagtcc ttgaccagtg aaagtcagcg
     6961 ttaccaaaaa attgacaagg aatttgtgga ttatctgaag aaatccttta cctatagtcg
     7021 agcagagagt cagcaaaagg ctctgaaaaa ggttgcccat ctcttgctta atgtcggcat
     7081 tctctggatg ggggctgttc tggtcatgga tggcaagatg agtttggggc agttgattac
     7141 ctataatacc ttgctggttt actttaccaa tcctttggaa aatatcatca atctgcaaac
     7201 caagcttcag acagcgcagg ttgccaataa ccgtctaaat gaagtgtatc tagtagcttc
     7261 tgagtttgag gagaagaaaa cagttgagga tttgagcttg atgaagggag atatgacctt
     7321 caagcaggtt cattacaagt atggctatgg tcgagacgtc ttgtcggata tcaatttaac
     7381 cgttccccaa gggtctaagg tggcttttgt ggggatttca gggtcaggta agacgacttt
     7441 ggccaagatg atggttaatt tttacgaccc aagtcaaggg gagattagtc tgggtggtgt
     7501 caatctcaat cagattgata aaaaagccct gcgccagtac atcaactatc tgcctcaaca
     7561 gccctatgtc tttaacggaa cgattttgga gaatcttctt ttgggagcca aggaggggac
     7621 gacacaggaa gatatcttac gggcggtcga attggcagag attcgagagg atatcgagcg
     7681 catgccactg aattatcaga cagaattgac ttcggatggg gcagggattt caggtggtca
     7741 acgtcagaga atcgctttgg cgcgtgctct cttgacagat gcgccggtct tgattttgga
     7801 tgaggcgact agcagtttgg atattttgac agagaagcgg attgtcgata atctcatggc
     7861 tttggacaag accttgattt tcattgctca ccgcttgact attgctgagc ggacagagaa
     7921 ggtggttgtc ttggatcagg gcaagattgt cgaagaagga aagcatgctg atttgcttgc
     7981 acagggaggc ttttacgccc atttggtcaa tagctagaaa gaggagagga tgaaaccaga
     8041 atttttagaa agtgcggagt tttataatcg tcgttaccat aatttttcca gtagtgtgat
     8101 tgtacccatg gcccttctgc tcgtgttttt acttggcttt gcaactgttg cagagaagga
     8161 gatgagtttg tccactagag ctactgtcga acccagtcgt atccttgcaa atatccagtc
     8221 aactagcaac aatcgtattc ttgtcaatca tttggaagaa aataagctgg ttaagaaggg
     8281 ggatctcttg gttcaatacc aagaaggggc agagggtgtc caagcggagt cctatgccag
     8341 tcagttggac atgctaaagg atcaaaaaaa gcaattggag tatctgcaaa agagcctgca
     8401 agaaggggag aaccactttc cagaggagga taagtttggc taccaagcca cctttcgcga
     8461 ctacatcagt caagcaggca gtcttagggc tagtacatcg caacaaaatg agaccatcgc
     8521 gtcccagaat gcagcagcta gccaaaccca agccgaaatc ggcaacctca tcagtcaaac
     8581 agaggctaaa attcgcgatt accagacagc taagtcagct atggaaacag gtgcttcctt
     8641 ggccagtcag aatctagcct actctcttta ccagtcctac aagtctcagg gcgaggaaaa
     8701 tccccaaact aaggttcagg cagttgcaca ggttgaagca cagatttctc agttagaatc
     8761 tagtcttgct acttaccgtg tccagtatgc aggttcaggt acccagcaag cctatgcgtc
     8821 agggttaagc agtcaattgg aatcccttaa atcccaacat ttggcaaagg ttggtcagga
     8881 attgaccctt ctagcccaga aaatcttgga ggcagagtca ggtaagaagg tacagggaaa
     8941 tcttttagac aaggggaaaa ttacggcgag tgaggatggg gtgcttcatc ttaatcctga
     9001 gaccagtgat tctagcatgg ttgcagaagg tgccctacta gcccaacttt atccatcttt
     9061 ggaaagagaa gggaaagcca aactcacagc ctatctaagt tcaaaagatg tagcaagaat
     9121 caaggtcggt gattctgttc gctatactac gactcatgat gccgggaatc aacttttcct
     9181 agattctact attacaagta ttgatgcgac agctactaag actgagaaag ggaatttctt
     9241 taaaatcgag gcggagacta atctaacttc ggagcaggct gaaaaactta ggtacggggt
     9301 ggaaggccgc ttgcagatga ttacgggcaa gaaaagttat ctacgttatt atttggatca
     9361 atttttgaac aaagagtaat gttcgtgttt ttagagttaa ataattttta aactgtgaga
     9421 aagattcttc ttgcagtttt ttctttacaa tttttaaaaa aacatctgct atttattcgg
     9481 ttaaattctt gtgttttttg gttttttgtg gtaaaatgtg ctcaagtaat acgaaaggcg
     9541 aactttaaaa tgtcaaaaca attgatctat tcgggaaaag ctaaagatat ctatacaact
     9601 gaggatgaaa atcttattat ttcaacttac aaggaccagg cgactgcttt caacggtgtc
     9661 aagaaggagc agattgcagg taagggagtc ttgaataatc agatctcatc ttttattttt
     9721 gagaaattaa atgcggctgg tgtggcgact cactttgtgg agaaactttc agacacggaa
     9781 caactcaata aaaaggttaa gattattcct ttggaagtcg tgctccgcaa ctatactgct
     9841 ggttcctttt caaaacgttt tggtgtggat gagggaatcg ccttggagac tccgattgtc
     9901 gaattttact acaaaaatga tgatttggat gatccattta tcaatgatga gcatgtgaaa
     9961 ttcctacaga ttgcgggtga ccagcagatt gcctacttga aggaagaaac gcgtcgtatc
    10021 aatgaactat tgaaagtctg gtttgctgag attgggctta aattgattga ctttaagcta
    10081 gagttcggtt ttgacaagga tggcaagatt atcttggcag acgaattttc accagataac
    10141 tgccgcttgt gggacgctga tggcaaccac atggataagg atgttttccg tagaggattg
    10201 ggagaactaa ccgacgttta tgagattgtt tgggaaaagt tgcaggaatt gaaataatct
    10261 gtttgcaacg gaaaaccttc gtctctcaac taaaaggact caggctgaaa aggtccccca
    10321 gaccttttca ctctgtagag aactaggtga actaacagat gtttacgaaa ttgtctggga
    10381 aaagttgcag ggtttaaaat aacaacctca aggctgtttg ggaatattgc aagagctgaa
    10441 ataaaggaat aagaattgat ggataaacgt atttttgttg aaaaaaaggc tgattttcag
    10501 gtcaagtcag agagtttggt tagagagctc cagcacaact tgggactgtc aagcttgaaa
    10561 agtattcgta ttgtgcaagt atatgatgta tttgacttgg ctgaggactt gtttgcacct
    10621 gcagagaagc acattttctc tgagcaggga accgaccatg ttttagatga agtatctgtg
    10681 caggcggatc ttgctaacta tgctttcttt gccattgaaa gtctgccagg gcagtttgac
    10741 cagcgtgcag cttcgtcaca ggaagccttg cttttgttgg gaagttcgag tgacgtgaca
    10801 gtcaacacag cccaacttta cttggtgaat aaagatattg atgcgactga gttggaagct
    10861 gtcaaaaact acctgctcaa tccagttgat tctcgtttca aggatatcac gacagggatt
    10921 gccaagcagg agttttcaga gtcagacaag accattccaa aattgacttt ctttgaaagc
    10981 tatacagcag aagactttgc tcgctacaag gccgagcaag ggatggccat ggaagtggat
    11041 gatttgctct ttatccaaga ctactttaag tcaatcgggc gcgtgccaac tgagactgaa
    11101 ctcaaggttt tggacactta ctggtctgac cactgccgtc atacgacttt tgagacagag
    11161 ttgaaacaca tcgacttttc agcttctaaa tttcaaaagc aattgcagtc aacctatgac
    11221 aagtatattg ccatgcgcga ggagttaggt cggtctgaaa aaccacaaac cttgatggat
    11281 atggcgacta ttttcggtcg ttatgagcgt gctaacggac gattggatga tatggaagtc
    11341 tctgacgaaa tcaatgcctg ctcagttgaa attgaagtgg atgttgatgg tgtcaaggaa
    11401 ccttggctcc tcatgtttaa aaatgaaacc cacaaccatc caacagaaat tgagccattt
    11461 ggtggagcgg ctacctgtat cggtggagct attcgtgatc cgttgtcagg ccgttcctat
    11521 gtttaccaag ccatgcgtat ttcaggtgct ggtgatatta cagcaccgat ttcggaaact
    11581 cgtgctggga aattgccaca acaagtcatt tctaaaacag cagctcatgg ttattcttca
    11641 tatggtaacc agattgggct tgccacaacc tacgttcgtg aatacttcca cccaggcttt
    11701 gtagctaaac gtatggaact tggtgccgtt gttggtgcgg ctcccaaggg caatgttgtc
    11761 cgtgaaaaac ctgaagcagg tgatgtgatc atccttctcg gaggcaaaac aggtcgtgat
    11821 ggtgtcggtg gtgcgacggg ctcttctaag gttcaaacag ttgagtctgt agagactgct
    11881 ggtgctgagg ttcaaaaagg aaatgccatc gaagaacgca agattcagcg cctcttccgt
    11941 aatggcaatg tcactcgtct gatcaagaag tccaatgact ttggggcagg cggcgtctgt
    12001 gtggctatcg gtgaattggc agacggtctt gaaatcgacc tcaacaaggt gcctcttaaa
    12061 taccagggct tgaatggtac agaaattgcc atctctgaat cacaagaacg gatggcggtc
    12121 gtggttcgtc ctgaagatgt ggataccttc gttgccgaat gtaacaaaga aaatattgat
    12181 gctgttgtgg tggcgacagt aactgaaaaa ccaaatcttg tcatgcactg gaatggtgag
    12241 acaatcgttg acttggagcg tcgtttcctt gacaccaatg gtgtgcgcgt ggttgtcgat
    12301 gccaaagttg tggacaagga tgtcaaactc ccagaagagc gtcaaacatc tgctgaaaca
    12361 ctggaatcag atacccttac ggttctatct gacctcaacc atgcaagtca aaaaggatta
    12421 cagactatct ttgactgctc tgttggacgc tcaacggtta atcacccact tggtggtcgt
    12481 taccaactca caccaactga ggcatctgtg cagaaattgc cagttcaaca cggtgtgact
    12541 catactgcgt cggtcattgc tcaaggtttc aacccatatg tagctgaatg gtctccatac
    12601 cacggtgctg cttatgcggt tatcgaagca actgctcgtt tggtggctgc tggtgccaac
    12661 tggtctaagg ctcgtttctc ttaccaagaa tatttcgagc ggatggacaa gcaagcagag
    12721 cgtttcggtc agccagtagc tgctcttcta ggttctattg aagcacaaat tcagcttggc
    12781 ttgccatcta tcggtggtaa ggactccatg tctggtacct ttgaagaatt gaccgttccg
    12841 ccaaccttgg ttgcctttgg ggtgacgacg gcagatagcc gtaaggtgct ctctccagaa
    12901 tttaaagctg ttggggaaaa tatctactac atcccaggtc aagccctctc tgcagagatt
    12961 gattttgact tgattaagaa aaattttgct cagtttgaag caagccaagc tgaccataaa
    13021 gtgacgtctg catcagctgt caaatacggt ggtgtagttg aaagtttggc tcttgctacc
    13081 tttggaaact atattggtgc agaggtgacc ttgcctgaac ttaaaacagc tttgacagct
    13141 caattaggcg gctttgtctt cacatctcct gaagaaattg ctggagtaga gaagattgga
    13201 caaacgaaag cagactttac actgactgtc aacggtgtga agctagatgg acacaagctt
    13261 gacagtgcat ttcaagggac attggaagaa gtttacccaa cagaatttac ccaagcgaaa
    13321 gaactagaag aagtaccagc tgtggcatca gatgttgtga ttaaagccaa agaaaaggtt
    13381 gaaaaacctg tggtttacat cccagtcttt ccaggaacca actcagaata tgattcagct
    13441 aaggccttcg aaaaagaagg tgcagaggtc aatttggtgc cattcgtgac cttgaatgaa
    13501 gaagctattg tcaagtcagt tgaaactatg gttgacaata tcgacaagac taatattctc
    13561 ttctttgccg gtggattctc ggctgcggat gaaccagatg gttcagctaa gtttatcgtc
    13621 aatatcctgc ttaatgaaaa agtgcgtgtg gctattgata gctttatcgc ccgtggtggc
    13681 ttgattatcg gtatttgtaa tggattccaa gccttagtca aatcgggtct cctaccatac
    13741 ggaaactttg aagatgctaa cagtactagt ccaaccctct tctacaatga tgccaaccaa
    13801 cacgtgacca agatggtgga aactcgcatt gccaatacca actcaccatg gttggctggt
    13861 gtgcaagtgg gcgatatcca cgctattcct gtttcgcacg gtgaagggaa gtttgtcgtg
    13921 acggctgagg aatttgcaga gctccgtgac aatggacaaa ttttcagcca atacgttgac
    13981 tttaacggta aaccaagtat ggattctaag tacaatccga atggttctgt ccatgccatc
    14041 gaaggaatta ccagcaagaa tggtcaaatc atcggtaaga tgggccactc agaacgttat
    14101 gaggatggtc ttttccaaaa tatcccaggc aataaagacc aacacctgtt cgcatcagcg
    14161 gttaaacatt tcactggaaa ataagactta cagattttct aatagatagt atcagtaatg
    14221 taaaagtcat gtaaatctag ctcttgatga ttacaaatga aaattaggta taaaaaatga
    14281 catacgaagt aaaatctctt aatgaagaat gtggtgtttt cggtatttgg ggacatccag
    14341 atgctgctaa gttgacctat tttggactcc acagtcttca acaccgtggt caggaggggg
    14401 caggaatcct ctccaatgat caaggacaac tgaagcgcca tcgtgacatg gggcttttat
    14461 cagaagtttt cagaaatcca gccaatttgg ataaattgac aggagctggt gcgattgggc
    14521 atgtgcgtta tgcgactgct ggcgaagctt ctgttgataa catccagccc ttcctcttcc
    14581 gttttcacga tatgcagttt ggcttggctc ataatggaaa tctgaccaat gcagcctctc
    14641 tcaagaaaga actggaacaa agaggagcaa ttttcagcgc gacttcggac tctgaaatct
    14701 tggctcacct cattcgtcgt agtcataatc ctagcctgat gggcaaaatc aaggaagcgc
    14761 tcagccttgt caaaggtggt tttgcctata tcttgctgtt tgaggacaag ttgattgcgg
    14821 ctcttgaccc taatggcttt cgtccgcttt ctatcgggaa aatggccaac ggagcggtgg
    14881 ttgtttcctc tgaaacctgt gcttttgagg tcattggtgc cgagtggatt cgtgatttga
    14941 agccaggtga gattgtgatc attgatgacg agggcattca gtatgacagc tatacagatg
    15001 atactcagtt ggcgatttgt tctatggagt atatctactt tgctcgccct gattctaata
    15061 tccacggtgt caatgtccat acggcacgta agagaatggg agcgcaattg gcgcgagaat
    15121 ttaagcatga ggcagatatt gtagttggtg tgcccaattc ttccctaagc gcggctatgg
    15181 gatttgcgga agaatcaggc ttaccaaatg aaatgggtct gatcaaaaac caatacaccc
    15241 agcgaacttt tatccaaccg actcaagaat tgcgggagca aggagtgcgg atgaaactgt
    15301 ctgctgtttc gggtgttgtc aaaggcaaac gtgtggtcat ggtggatgat tccattgtac
    15361 gtggaacaac ctctcgtcgt atcgttcagc tcttgaaaga agcgggtgcg actgaggttc
    15421 acgttgccat tggaagtcct gcactagcgt atccatgttt ctacgggatt gatatccaga
    15481 cccgtcagga gctgattgca gccaatcata cggtcgaaga aactcgccaa atcattggtg
    15541 cggacagtct gacttatctt tcaattgatg gcttgattga gtcgattggt atcgaaacag
    15601 atgcgccgaa cggtggtctc tgtgtcgctt actttgacgg tgactaccca acgcctctct
    15661 atgactacga agaagactat cgtagaagtt tggaagaaaa gaccagtttt tacaagtagg
    15721 cgacagattc tccattaaag aaaaggaaaa aacaaatgac aaataaaaat gcatatgccc
    15781 agtcgggtgt ggatgttgaa gcgggttatg aagttgttga acggattaaa aagcacgtgg
    15841 cccgtacgga gcgtgcaggt gtcatgggag ctcttggtgg ctttggtggt atgtttgacc
    15901 tttccaagac tggggttaaa gaacccgtct tgatttcagg gactgacggt gtcggaacca
    15961 agctcatgtt ggctatcaag tacgacaagc acgataccat cgggcaggac tgtgtggcca
    16021 tgtgtgtcaa cgacatcatt gctgcaggtg cggaacccct ctattttctc gactacgtag
    16081 cgacagggaa gaatgaacca gctaagctag aacaagtggt tgctggtgtg gcagaaggtt
    16141 gtgtgcaggc tggtgctgcc ctcatcggtg gggaaacggc tgaaatgccg ggcatgtacg
    16201 gcgaagacga ctatgacttg gctggttttg cggtcggtgt ggctgaaaaa tctcaaatca
    16261 ttgacggttc aaaggtggta gagggagatg ttcttctcgg acttgcttca agtgggattc
    16321 actcaaatgg ttactctttg gttcgtcgtg tctttgcgga ttacacaggt gaggaagtcc
    16381 taccagaatt ggaaggcaag aaacttaagg aagttctact tgagccgact cgtatctatg
    16441 tcaaggctgt cttgccgctc atcaaagaag agttggtcaa cggcattgcc cacatcacag
    16501 gtggtggctt tatcgaaaat gtccctcgta tgtttgcaga tgacctagct gctgaaattg
    16561 atgaaagcaa agttccagtg cttccaattt tcaaagccct tgaaaaatat ggtcagatta
    16621 aacacgaaga aatgtttgaa atcttcaata tgggtgtggg acttatgttg gcggtcagcc
    16681 ctgaaaatgt agagcgtgta aaagaattgt tggatgaagc agtctatgaa attggtcgca
    16741 tcgtcaagaa agaaaacgaa agtgtcatta tcaaatgaaa aaaatagcgg tttttgcctc
    16801 tggtaatggc tcaaattttc aggtgattgc cgaagaattt ccagtggagt ttgtcttttc
    16861 agaccatcgt gatgcctatg tgcttgagcg tgcaaagcag ctcggcgttc tgtcctatgc
    16921 ttttgaactc aaggagtttg agagcaaggc agactacgaa gcagcccttg tcgaactctt
    16981 ggaagaacac cagattgcct tggtttgcct agcaggctac atgaaaatcg ttggaccaac
    17041 cttattgtcg gcttatgaag gtcggattgt caacattcat ccagcctact tgccagaatt
    17101 tccaggagct catgggattg aggatgcttg gaatgctggc gtgggtcagt ctggtgtgac
    17161 cattcactgg gtggattcgg gtgtggatac aggccaggtc atcaaacagg ttcgtgtgcc
    17221 acgactagct gatgatacca ttgacagatt tgaagctcgc atccatgaag cagagtacag
    17281 gttgtatccg gaagtagtga aggctctatt tacagattga ctttttgatg attcatatga
    17341 tatctttgat tttaaattgg agtcagtgtt tgttgaagac ggcttcaaac ggaggtattt
    17401 gtaatgttag aatctaaaaa aacaactcga tatgtatttt atgtctatct gatgttatta
    17461 acttggggaa tcttatttaa gtttgaaaca aatcctgaat ttatagcatt tttcttagct
    17521 ccaaggtata tcaattggat tccattttca gaaccactaa tagtcgatgg aaaaattgtt
    17581 tttgctgaaa tgttatttaa tctgattttc tttattccat taggtgtttg tttccctttg
    17641 ataaaaacta atttatctag tttaagaata gtcgggacag gtttcttgat tagtttattg
    17701 tttgagtgct tacagtatat tttagcaata ggtataacag atataacgga tttgacttta
    17761 aatacgctag gtgtctgtgt aggcttactg atttatcaaa tttttataag agtgttcaaa
    17821 tcacagacta gaaaatggat caatatctta ggtatgctta gccttggttt tgcttatctt
    17881 gttttactgt tactgcattt aattggtgtt taactaatga ttaaaaagga gaatataatg
    17941 actaaacgcg tcttaatcag cgtctcagac aaagcggaca ttgttgaatt tgcccaagaa
    18001 ctcaaaaaac ttggttggga gattatctcg acaggtggaa ctaaggttgc ccttgataat
    18061 gctggggtgg ataccattgc tatcgatggt gtgactggtt tcccagaaat gatggacggt
    18121 cgtgtgaaga ccctccaccc aaatatccac ggagggcttc tcgctcgtcg tgacttggat
    18181 agccacttgg aagcggctaa ggacaacaag attgagctca ttgaccttgt ggtggtcaac
    18241 ctttacccat ttaaggaaac tatccttaaa ccagatgtga cttatgatga tgcagttgaa
    18301 aatatcgata ttggtgggcc atctatgctt cgttcagcag cgaaaaatca tgccagtgtt
    18361 acagttgtgg tagatcctgc tgactacgct gtggttttgg acgaattggc agcaaacggc
    18421 gaaacctctt atgaaactcg ccaacgttta gcagccaaag tatttcgtca cacagcggct
    18481 tatgacgcct tgattgcaga atacttcaca gctcaagtgg gtgaaaacaa gcctgaaaaa
    18541 ctcactttga cttatgacct caagcaacca atgcgttacg gtgagaatcc tcaacaagac
    18601 gcggactttt accagaaagc tttgcctaca gactactcca ttgcttcagc caaacagctc
    18661 aacgggaaag aattgtcatt taataatatc cgtgatgcag atgctgctat ccgtatcatc
    18721 cgtgacttca aagatagtcc aaccgttgtg gctctcaaac acatgaatcc atgtggaatt
    18781 ggtcaagctg atgacatcga gactgcttgg gactacgctt atgagtctga cccagtgtct
    18841 atctttggtg ggattgtcgt cctcaaccgt gaggtggatg ctgcgacagc tgagaagatg
    18901 cacggcgttt tcctcgaaat catcattgca ccaagctata cggatgaagc gctagccatt
    18961 ttgatcaata aaaagaaaaa cttgcgtatc cttgccttgc catttaatgc tcaagaggct
    19021 agcgaagtgg aagcagaata cacaggtgta gtcggtggac ttctcgtgca aaatcaagac
    19081 gtggtcaagg aaagcccagc tgactggcaa gtggtgacta aacgtcagcc aactgagaca
    19141 gaagcgactg ctcttgagtt cgcttggaag gctatcaagt acgtcaaatc aaatggtatt
    19201 atcgtgacca acgaccacat gacacttggt gttggtccag gtcaaaccaa ccgtgtggct
    19261 tctgttcgcc ttgccattga ccaagccaaa gatcgtctgg acggggcggt ccttgcttca
    19321 gatgccttct tcccatttgc ggataacgtg gaagaaatcg ccaaagcagg aattaaggcc
    19381 atcatccagc ctggtggctc tgtccgtgac caagaatcca tcgaagcagc ggataaatac
    19441 ggcttgacta tggtctttac aggtgtgaga cattttagac attaagaaga taaaagggaa
    19501 gaaaacagtt tctttccttt tttggcttaa aatgctaact gaaacaagat taaaacgaac
    19561 ttttttgata taatgttggt aaataattcg caaaagaggt tgaggaatga aactgcttgt
    19621 tgtcggttct ggtggtcgtg agcatgcgat tgctaaaaag ttacttgaat caaaagacgt
    19681 ggaaaaagtc tttgtagctc ctgggaatga tgggatgact ctggatggtt tggaattggt
    19741 aaatatctct atttccgaac attctaaatt gattgacttc gcaaagacca atgatgttgc
    19801 ttggaccttt atcggtccag atgatgccct tgctgctggt atcgtggatg attttaacca
    19861 agctgggctt aaggcctttg gtccgactag ggctgcagcg gagctggagt ggtccaagga
    19921 ttttgccaag gaaatcatgg tcaaatatgg tgtttcgaca gcagcctatg gcacattttc
    19981 agatttcgag gaagcaaagg cctatatcga aaagcatggt gcgcctatcg tagtcaaggc
    20041 ggatggcttg gcgcttggga agggtgtcgt cgtttctgag acggttgagc aagcggtcga
    20101 agccgctcat gagatgcttt tggacaataa atttggtgac tcaggtgcgc gcgtggttat
    20161 tgaggaattc tttgaaggag aggaattttc actctttgcc tttgtcaatg gtgataagtt
    20221 ctacatcatg ccaacggctc aggaccacaa acgtgcctat gatggcgaca aagggcctaa
    20281 cacgggtggt atgggtgcct atgcgccagt tccacactta ccacagagtg tagttgatac
    20341 agcggttgac accattgtca agccagttct agaagggatg attaaagaag gtcgccctta
    20401 tctgggagtt ctttacgcag ggcttatcct gacagctgat ggaccgaaag tcattgagtt
    20461 caacgctcgg ttcggagatc cagaaactca gattatcttg cctcgcttaa cctctgactt
    20521 tgctcaaaat atcacagata tcctggatag caaggagcca aatatcacgt ggacggacaa
    20581 gggtgtgact ctgggtgtgg ttgtcgcatc caagggctac ccgctaaatt attcaaaggg
    20641 tgttgagttg ccagtcaaaa ccgatggcga catcattacc tactatgcag gggctaagtt
    20701 tgcggaaaat agcagagcac tgctctcaaa cggcggacga gtttatatgc tcgttaccac
    20761 agcagatacc gtcaaagaag cccaagccag catctaccaa gaactatccc aacaaaaaat
    20821 agaaggactc ttctaccgaa cagatatcgg aagcaaggca attaagtaaa gatataagaa
    20881 taacgcgccg tagtcgccaa acacgataat ggtcgtcgtg gtgaaaagac cagaacagtg
    20941 aatgttctgg tcagggggaa acttggagac cgtaggctca aagtttagga atgaaaccga
    21001 aggtttgctt ccgcctccat cacctaagac cattatcaaa aagaaaaata aaaattcaca
    21061 aaatacgtta atgatcgtat ggtttgcgag cgttagcgag ctaatataga acaatcaccg
    21121 ccgttgtgaa agaacgattg gatgataatc caatcgttca gggaaattgg aagaccttgg
    21181 gtttccaatt taggcatgag acacctttgg tggctgctgc cgtccctcac aagctaaggt
    21241 gattgttgaa aaagaggaaa aaggagaaga aatgaaacca gtaatttcca tcatcatggg
    21301 ctcaaaatcc gactgggcaa ccatgcaaaa aacagcagaa gtcctagacc gcttcggtgt
    21361 agcctacgaa aagaaagttg tttccgcaca ccgcacacca gacctcatgt tcaaacatgc
    21421 agaagaagcc cgtagtcgtg gcatcaagat catcatcgca ggtgctggtg gcgcagcgca
    21481 tttgccaggc atggtagctg ccaaaacaac ccttccagtc attggtgtgc cagtcaagtc
    21541 tcgtgctctt agtggagtgg attcactcta ttctatcgtt cagatgccgg gtggggtgcc
    21601 tgttgcgacc atggctatcg gtgaagctgg agcgactaac gcagctctct ttgccctccg
    21661 tctcctctct gtagaagata agtccattgc ggatgcactt gccaactttg ctgaagaaca
    21721 aggaaaaatc gcagaggagt cgtcaaatga gctcatctaa aacaatcgga attatcggtg
    21781 gcggtcaact gggtcagatg atggccattt ctgctatcta catgggccac aaggttatcg
    21841 cgctggatcc tgcggcggat tgcccggcct ctcgcgtggc ggaaatcatt gtggcacctt
    21901 ataacgatgt ggacgccctc cgtcagttgg cagaccgttg cgatgtcctc acttatgagt
    21961 ttgaaaatgt cgacgctgac ggtttggatg ccgttatcaa ggatggacaa ctccctcaag
    22021 gaacagatct gctccgcatt tcgcaaaatc gtatttttga aaaggacttt ttgtcaaaca
    22081 aggctcaagt cactgtggca ccctacaagg tcgtgacttc tagcctagac ttggcagata
    22141 tcgacttgtc gaaaaactat gtcctcaaga ctgcgactgg tggctacgat ggtcatggac
    22201 aaaaggttat tcgttcagaa gcagacttgg aagcagccta tgcgctagca gactcagcag
    22261 actgcgtctt ggaagaattt gtcaactttg accttgagat ttctgtcatc gtgtcaggaa
    22321 atggcaagga ggtgacgttt ttcccagttc aggaaaatat ccaccgcaac aatatcctgt
    22381 ctaagaccat cgtaccagcc cgcatttctg aaagtctagc agacaaggct aaagctatgg
    22441 cagtgcgaat cgcagaacaa ctcaacttgt ctggaactct ctgtgtggaa atgtttgcga
    22501 ctgatgatga cgttattgtc aatgaaatcg ccccacgacc acataactct gggcactatt
    22561 ctattgaagc ctgtgatttc tctcagtttg acacccatat tctgggtgtt ctcggagcac
    22621 cattaccagt catcaaactc catgcgccag ccgttatgct taatgtcctc ggtcagcatg
    22681 tcgaggctgc tgagcaatat gtgacagaaa attcaagcgc ccacctccac atgtatggta
    22741 aaatagaagc gaagcacaac cgcaagatgg gacatgtgac cttgtttagt gatgtaccgg
    22801 atagtgtgga tgagcttggg gaagggattg atttttagga caagtctatg atacaaatta
    22861 tcgttaatac atttattgaa aaggataaga ctggagcagt tgttgaagtg ttgtatgcca
    22921 gtgctgacca agataaggta caagctaaat atgaagaact agctgcacaa taccccgaaa
    22981 attatttagc tatctatgat gtaccgctgg atacggattt gaatacacta gatcattacc
    23041 cgtctgtgtt tattgaaaaa gaggagtttg agtagaaatc ttggtttacc tagatagctt
    23101 attcccaaca gcttaagaag aaaggaaaaa ttaacacatg atcaaccgtt actctcgccc
    23161 tgagatggcg aatatttgga gtgaagaaaa taaataccgt gcttggcttg aggtggaaat
    23221 cctctctgac gaggcatggg ctgagttggg ggaaatccct aaggaagatg tggctttgat
    23281 tcgcaagaag gcggactttg acatcgaccg tattttggaa attgagcagg agacgcgcca
    23341 cgatgtggtg gctttcacgc gtgcggtttc tgagactctt ggtgaagagc gcaagtgggt
    23401 tcactatggg ttaacttcta ccgacgtggt ggatactgct tatggttacc tctacaagca
    23461 ggccaacgac atcatccgtc gtgaccttga aaacttcact aatatcatcg ctgacaaggc
    23521 caaggagcac aagttcacca tcatgatggg gcgtactcat ggtgtgcacg ctgagccgac
    23581 aacctttggt cttaaattgg caacttggta cagcgaaatg aaacgcaata tcgagcgttt
    23641 cgagcatgca gctgctggtg tagaagctgg taagatttct ggtgcggttg ggaactttgc
    23701 caatatccca ccatttgtag aggagtatgt ctgcgataaa cttggcatcc gtgcccaaga
    23761 aatctctaca caagtccttc ctcgtgacct tcacgctgag tactttgcgg ttcttgccag
    23821 cattgcgact tcaatcgaac gtatggcgac tgagattcgt ggtctacaaa aatctgagca
    23881 acgcgaagta gaagagttct ttgctaaagg gcaaaaaggg tcttcagcaa tgcctcacaa
    23941 acgcaaccca atcggttctg aaaatatgac tggtctggcg cgtgtcattc gtggtcacat
    24001 gattacggct tatgaaaacg tcgctctctg gcacgaacgc gatatttctc actcatcagc
    24061 tgagcgtatc atcacaccag atacgaccat tttgattgac tacatgctca accgttttgg
    24121 aaatatcgtc aagaacttga cagtcttccc agaaaatatg atccgaaaca tgaactcgac
    24181 ttttggtctt atctttagcc aacgggctat gttgacattg attgaaaaag gcatgacccg
    24241 tgagcaagcc tatgacttgg tgcaaccaaa aacagcctac tcttgggaca accaagtaga
    24301 ctttaaacca cttcttgagg cagattcaga agtaacatca cgtctcactc aagaagaaat
    24361 cgatgaaatc ttcaacccag tttattacac caaacgagtg gatgatatct ttgaacgtct
    24421 tggactaggt gattaattaa aaaataaaca gcgagcttca atctcgctgt ttatttttta
    24481 tcgaaaagac ttagtcttct tttcttttag tgagtccata ggctgctagt gtggccatga
    24541 gtcctgcgac tactagtcct gcagaatcgt gagttcctgt ttcaggaagt tttttctcta
    24601 ttaccacagg agctggatct tgaggaagag ctttgctttc ctcagcagga gcagttgatg
    24661 gagctggttg gcttgggatt tctagttttg gtttttcttc agcaatagcg gcttgtccgt
    24721 tttcatcgcc tacatgtgtt accatagttc cgacttcgac tatttgagta acggcttcct
    24781 gtgctacgac actatttaca agtgttttca cttccttacc atcggcagaa gtgctcacag
    24841 agtagaagtt gctacgatgt ccattgacgc ccttagtaat gacttgtgtt tttcctttga
    24901 gtaagagtgg attttcacga gtcactgtgg taaatggaat ttcttctttt tggatatcca
    24961 gtctaggttt tacctcagta gttggtgcaa gaccactttc atcacccttg tgagttacag
    25021 gagcgccaac ttcaaccact tggtttataa cttctttggt tacctggcta tcaaggactg
    25081 tttctgttgt ttttccattt tcagtgagta cagagatgta atgagttcgc tcacctttga
    25141 ctcctgctgt gataatattt tcctgaccag ctgggaggtt aggattttct ttcttgataa
    25201 cttcaaatgg aatttcttca gttcttgtga tgagttctgg tctggtttca acattggcag
    25261 ccacttcatt ttcatccagg cttcctgaat gagttgcagc tggtttgagg ccttgaagag
    25321 cggcttttag gttggctaca agtgtgtcaa gctcagcttg tttattacgg ttgaggttgt
    25381 aatttagagc tgttttagct gcgtcaaggg cctcaagact ttctttacta tatccttcta
    25441 agtttgtagg aattttagct aattcttcgc ggagagcatt ataattagca cggaagtagt
    25501 ctttgttgtg gtctgcaaag gcagtcatga gttcaaagat ttcctcttcc ttgtattcag
    25561 cgcttggtct atctgcccag attgaaagca tacttccgac tgttggaaga tctacttcag
    25621 gatatttggt agaagctagt tgattgaatg gtgtttttcc agtattctca atagctttct
    25681 tgaggaaacc accaccatct tctggttttt gaccaagaat gtagtaccag tcaccgttgg
    25741 tattcaagaa tttatagcct ttgcttgcta ggtattgagg tgatgcgagg ttatatcccc
    25801 accagccttt agaccagtaa gaaatcaaga catctttgtc aaactgaaca tcgtccttgt
    25861 cttcatagta gaagccatcg ttgaaggcca ttggttgaag ccctctttct ttggccatag
    25921 ctgcgagggt gttggcatat tcggcaaatt tgccatagag ttgataccac ttgaggtagt
    25981 accagccttg ggcactagtc gcatcgttgg cgtattcgtc agtaccaaag ttgaaaatct
    26041 ttgttttacc tgcaaagaag tccatgtatt taccgatgag ggcttttaca aagttcatcg
    26101 cttcttcgtt tttcaagtcc atagttgttt ttgaaacttt atcaaagtgg gcttgaggat
    26161 ttttaatacc taatttttcc atggcaacca gcatagcatc catgtgacct ggactgttaa
    26221 tagctgggat gagaccgatg tccttagatt tagcgtattc aattagctct gttacttctg
    26281 cctgtgttag tgcagtaccg tttggatcgt cgtagtaagc tttagttcct tcgataatag
    26341 cttttttaac gtcatcacta gcataggttt ttccgttggc agtaatggtc atatcatcga
    26401 gtagaaagcg aagtccgtca tttcctagaa ggagatggac atcagaatat ccgagctcac
    26461 tggccttgtc tacgatgcgt ttgagctggt tcagagtaaa gtatttgcgt ccagcatcga
    26521 ttgagattac cttgtttttg gcaagttttt caacctcacg tttagcttct tcttcttttt
    26581 gagcttcagg cgtgagggtc aagttgttga cagtttcttg aagtttagca atggcttgat
    26641 caatcgtatc ttgttgggca cggctaaggt tgctatcgag agagcgaata gctttttcag
    26701 cttcttttac ggccgcgacg ctttctgcag tataacggtt caggtctttt ggtacctcgt
    26761 taagtgtttg ctctgcagat tcataatcag ctgcgaagta ttcagcgttg gcatttgcaa
    26821 aatgacgcat gagtttgaag aggcgtgatg gtgaataacg tgcagatgga gtgtcagccc
    26881 aagcagctac catactaccg atgattggga tatcagctcc ttctgttttt ggtacagaag
    26941 tgattggtgt gtttttaata ccattgagcc cctgatcgag attgtaccag ccttggccat
    27001 cagcgtttcg tccaagaacg tagtaccaag catcattggt attaaggatt tggtgacctt
    27061 tttcagctag tagtttagaa gaagcgacat cgtagcctcc ccaaccacca gtccacatag
    27121 aaacgatgat gtctttgtca aaactaccaa agcttgtgtc gctattgtag tagataccgt
    27181 cgttaaaggc cattggtttg agaccgtgcg attttacaat acgagcgagg tcattggcgt
    27241 aggcaataaa tttttcatag ccttttacag ggtagccttc gtttggatag tatttatcag
    27301 cttgaagcac actccaacct ttagcatctg tcgcatcatt ggcatattca tcaagtccga
    27361 tgttgaagat ttcagtcttt ttcgcgaaat aagcagcata cttgtcgata agggcttttg
    27421 taaaagcgac agcttgttcg ttgtcaagat tgacagtacg ggctgattcc ttcccaaaat
    27481 agctaaagtt agggttttgg attcccaatt ctttcatggc attgagaatc gcatccatgt
    27541 gaccaggact atttactgtc ggaatgagac cgataccttt atctttggca tagttaatca
    27601 gatctgtcat ttgactttct gttaagtgat tgccgtttgg atcgttgtaa taatcatttg
    27661 tacctttttc aatggcgcgt ttgacatcgt cactggcata ggtcttgccg ttagctgtga
    27721 tgctcatatc gtccaacatg aaacggagtc catcatttcc gactaatagg tgtaaatcag
    27781 tgtagccata atgtttcgct ttatcgatga tttccttgag ctgttctggt gagaaatatt
    27841 tacgtccagc atcaatagaa acaattttct ttttcgctag tttttcattt acagttgcag
    27901 cacgttcctt tcctgcctct gtttccggtt tgtcatcctc tgctttcgct tcatcttttt
    27961 tagctggttt atccttgtca gtcttgtctg tatttgactc tttagaatca acctctttcg
    28021 cttcttcctt tttagggcta gcttcttctg cctttttatt agcagtttct ttttcagcag
    28081 aagttggagt taccacttct gctttatcac taggagttga actaacttcc tcttgtggtt
    28141 tttcttctgt ttttggaaga ctagctacct tatcagtagc tggagtttct gtttctacag
    28201 tttttggagc ttctggttga agctctgctt taggtgtttc ctcagtccga ttttcggatg
    28261 attgagggga atcagaaacc gtatggatgg tcggttggtt ttctgtagta gtaggagtaa
    28321 ctccatcggc tgcaacagtc tgtgcttgga aggcaaatcc aattagaaca gaagctgctc
    28381 ctacagcgta tttacgaata gaaaaacgct gttgtttttc atgtttcatt gcaaaacctc
    28441 ctgattgcat tgttatattg atagcgatta tataaatcaa cgcctttatt ttatttctta
    28501 tattaacgag agtcaagagg agatgacaaa aaactataat aagtataaaa aaatataaaa
    28561 tttaaactta agatttcaga ttggtcggaa aaaatacgta tatatatcta gtataatttt
    28621 tggttctatt tctataaaat attccacaaa ttatagaatt ttccaaaaat aggtaagcgc
    28681 tacctttttg gtgtggtata ataagcatag aaaaagccca agcgattagc tcaggctttc
    28741 ttcttagtga tcacggtcac atgagataaa tttaatcttg tagtaatcag atcgtttgta
    28801 agtttcactg tattctaaaa cttggccagt tgattcgagt ttggtgattt tagtttgtag
    28861 gacagtaggg aattgttcat cgactccgag gactgaagcc gcatgttctg gagttggaaa
    28921 gactatttcg ttgatttctt caaagtgttc atcattcatg tgaatgtggt agtctaactt
    28981 gaaacgatta tagatagaac tatagtattc aaggtttgga taatttgcgt tgatatattg
    29041 ttctgggatg taggatgtat ggtagatata aacgacaccg tttgattcgc ggatacgttc
    29101 aatcttgtag tagaattgat cgccgcgtag acccaatttt tccaagtaaa caagcttgtt
    29161 tccgcgttca attgaaagaa cagttacctt atcatcttta gcattgaaga gttcaatatc
    29221 tgaaaactct acaagcttgt gtttgcgtgc acgtgaaacg aaggttcctt ttccttgttg
    29281 gcggacaata tagccatctt tggcaaggtc gtttaaggcg cgaacaactg tgatagagct
    29341 gacatcgtac attgaaatga gttctgcttc agtgtaaaat ttatctccac tgctaaactg
    29401 cccagagatg attttatttt ttaattcgtc ttttatgtat tgatatttgg gaattgccat
    29461 atttttacct ctattttcct tctccatagg aaattttacc ataaaagcga aaaaaatagt
    29521 agtcttttga ataaaaaatt agaataatag tttgaaaaat taagcgatat agggattgct
    29581 catattttat caatgtattc tatacaaaat tattttaggt cccatattcc gaatttaaag
    29641 agcgtttgaa atgggtctaa aaatgtttga aactaattga atgtgtgtat aatagtaaaa
    29701 atgcagtaaa tatcccgaca atttctattg acaattcaaa cagattggtt tataattaat
    29761 ataacaacaa atgaaagcgc aaactttcgc ggtcggaagg tagttttatg acacgatttg
    29821 agatacgaga tgatttctat ctcgatggaa aatcatttaa gattttatct ggtgccattc
    29881 attattttag ggttcctcca gaggattggt atcattcgct ctataacttg aaggctcttg
    29941 gttttaatac ggtagagact tatgttgctt ggaatttaca cgagcctcgt gaaggtgagt
    30001 ttcattttga aggtgatctg gatttagaga aatttctcca aatagcgcag gatttgggtc
    30061 tctacgcaat tgtgcgtccg tctccattta tctgtgcgga atgggaattc ggtggcttac
    30121 cagcttggct cttgaccaag aacatgcgaa ttcgctcatc cgacccagta tatatcgagg
    30181 cagttggtcg ctactatgat cagttattgc caagactggt gcctcgtttg ttggacaatg
    30241 gtggcaatat tctcatgatg caggttgaaa atgagtatgg ttcttacgga gaagataagg
    30301 cttacctgag agcgattcga cagctaatgg aagagtgtgg cgtaacctgt cccctcttta
    30361 catcagatgg tccatggcga gctactctga aagctggaac cttaattgaa gaggacctct
    30421 ttgtaacagg aaactttggt tctaaggcac cttacaactt ttcgcagatg caggaattct
    30481 ttgatgaaca tggtaagaaa tggccactca tgtgtatgga gttctgggat ggttggttca
    30541 atcgctggaa agaaccgatt atcacacggg atcctaagga attggcagat gcagttcgag
    30601 aggttttgga acaaggctct atcaatcttt acatgttcca cggtggtaca aactttggtt
    30661 tcatgaatgg ttgctcagct cgaggaactt tggacctgcc acaagttacg tcttatgatt
    30721 acgatgccct tctggatgaa gaaggaaatc caactgctaa atatcttgca gtcaagaaga
    30781 tgatggcaac acatttttca gagtatccgc agttggaacc actctacaaa gagagtatgg
    30841 agttggatgc tattccacta gttgaaaaag tttctttgtt tgaaacctta gatagcttgt
    30901 caagtcctgt agaaagtctc tatcctcaaa agatggagga gctgggacaa agttatggct
    30961 acctacttta tcgaacagaa acaaactggg atgcagaaga agaaagactt cgtatcattg
    31021 atggtcgaga tagggcccag ctgtatgtcg atggtcagtg ggttaaaact caatatcaga
    31081 cagagattgg ggaagatatt ttttatcaag gtaaaaagaa agggctatct aggttagata
    31141 tcttgataga aaatatgggg cgtgtcaact atgggcataa gttcttagcg gatacgcaac
    31201 gtaagggaat tcggacaggg gtctgtaagg atctgcattt cttactaaac tggaaacact
    31261 atccactccc actagacaat cctgagaaaa ttgatttttc aaaaggatgg actcaaggac
    31321 aaccagcctt ttacgcttat gactttacag tcgaagagcc aaaagatact tacctagact
    31381 tgtctgagtt tggtaagggg gttgcctttg tcaatgggca gaatctagga cgtttttgga
    31441 acgttggccc aactctctca ctttatatcc ctcatagcta tctcaaggaa ggtgccaacc
    31501 gcatcattat ctttgaaaca gaaggtcaat ataaagaaga gattcattta actcgtaaac
    31561 ctacactaaa acatataaag ggggaaaact tatgacaatt gtaggatgcc gtattgatgg
    31621 acgtttgatc cacggacaag tagccaatct ttgggctgga aaactaaatg tttcacgcat
    31681 tatggttgta gacgacgaag ttgtcaacaa cgatattgaa aagagtggtt tgaaacttgc
    31741 gacaccacca ggtgtgaaat tgagtatttt gccagttgag aaagctgcag ccaatattct
    31801 tgctggtaaa tacgatagcc aacgtctctt tatcgtggct cgtaaaccag accgcttcct
    31861 tggtttggta gaagcaggtg taccacttga aacccttaat gttgggaata tgtctcaaac
    31921 accagaaaca cgttctatta cacgttctat caacgtagta gacaaggatg tggaagactt
    31981 ccacaaactg gcagaaaaag gtgttaaact tactgctcag atggttccaa atgatccaat
    32041 ttcagacttt ttgagcttat taaaatagga aaaaaatttt taggaggtca ttgttatgat
    32101 acaatggtgg caaattttac ttctcacttt gtactcagct tatcaaatct gtgatgagtt
    32161 gacgatcgtt tcatctgcag gttctcctgt atttgctggt ttcattactg gtttaatcat
    32221 gggagatgtg actactggtt tacttatcgg tggtaacttg caactgttcg ttcttggggt
    32281 tggtaccttc ggtggtgctt ctcgtatcga cgcaacttct ggtgcggttc ttgcgacagc
    32341 cttctctgtt tcacaaggaa ttgatgcacc gcttgccatt actacaatcg ctgtaccagt
    32401 agcagctctc ttgacttact tcgacgttct tggtcgtatg actactacct tcttcgctca
    32461 ccgtgtggat gctgcaatcg aacgctttga ctataaaggt attgaacgca actacttgct
    32521 tggtgcgatt ccgtgggctc tatctcgtgc ccttccagtc ttctttgccc ttgcttttgg
    32581 tggtgccttt gtacaatcag tagtagactt cgttgaagcc tacaaatggg ttgcagatgg
    32641 cttgacactc gcaggacgta tgcttccagg tcttggattt gcaatcttgc ttcgttacct
    32701 tccagttaaa cgtaaccttc actaccttgc tatgggattt ggtttgacag ctatgttgac
    32761 tgttctttac tcatatgtaa caggtcttgg tggcgctgtt gctggtatcg taggtactct
    32821 tcctgctgaa gttgctgaaa aaattggttt cgtgaacaac ttcaaaggtt tgtctatgat
    32881 tggtatttct atcgtaggta ttttccttgc agtgcttcac ttcaaaaata gccaaaaagt
    32941 agctgtagca gcaccttcta caccatcaga aagtggggaa atcgaagatg acgaattcta
    33001 attacaaact tacaaaagaa gattttaatc aaatcaacaa acgtagcttg tttactttcc
    33061 aattaggttg gaactacgaa cgtatgcaag cttctggtta cctttacatg atcttgcctc
    33121 agttgcgtaa aatgtatggt gatggaactc ctgaattgaa agaaatgatg aaagttcata
    33181 ctcaattctt caatacttca ccattcttcc ataccattat cgctggtttt gaccttgcca
    33241 tggaagaaaa agatggtgta ggttcaaaag acgctgttaa cggtatcaag acaggtttga
    33301 tgggaccatt cgctcctctt ggggatacaa tctttggttc acttgtacct gctatcatgg
    33361 ggtcagtcgc agcaactatg gctatcgctg gccaaccttg ggggatcttc ctttggattg
    33421 cagttgcagt agcgtatgac atcttccgtt ggaaacagtt ggaatttgct tacaaagaag
    33481 gggttaacct tatcaacaac atgcaaagta ccttgacagc tttgattgac gctgcatctg
    33541 tacttggtgt cttcatgatg ggtgctcttg tagcaacagt gattaacttt gaaatttctt
    33601 acaagttgcc aatcggtgaa aagatgattg atttccaaga catcttgaac caaatcttcc
    33661 cacgtttgct tccagcaatc tttactgcct ttatcttctg gttgcttggt aaaaaaggta
    33721 tgaactctac taaagctatc ggtattatta tcgtacttgc tttggctctt tctgcccttg
    33781 gtcactttgc acttggaatg taattcctta tgactaaatc attaattttg gtgagccatg
    33841 gtcgcttctg tgaggagctt agaggtagca cagaaatgat tatgggccca caagacaaca
    33901 tttacacagt agctcttctt ccagaagatg gcccagaaga atttactgct aaatttgaag
    33961 ctgttattga aggattggat gatttcctag tctttgcgga tcttctcggt gggacacctt
    34021 gtaatgtggt gagtcgcttg atcatggaag gtcgtgatat tgacctttac gcagggatga
    34081 atcttccaat ggtgattgaa tttatcaatg cgagccttac aggcgcagat gcggactaca
    34141 agagccgtgc tgcagaaagc attgtgaaag ttaatgacct gttagcgggc ttcgatgatg
    34201 acgaagatga ataatactct tcgaaaatct cttcaaacta cgtcaacgtc gccttgccgt
    34261 aggtatatgt tactgacttc gtcagtctta tccggcaacc tcaaaacggt gttttgagct
    34321 gacttcgtca gtcttatccg gcaacctcaa agcagtgctt tgagcagcct gcggctagtt
    34381 tcctacagat tttagttgga actcgattca attcatgtga caacgtgaaa atcgttagag
    34441 cattttatat agaatataca tgggaatgga gcttactccc attcccatat ttaatagaaa
    34501 aagaggaact caatgctaca ttatacaaaa gaagacttgc tcgaattggg tgcagaaatc
    34561 actacgcgtg aaatctacca acagcctgat gtatggagag aagcttttga attttatcaa
    34621 gcaaaacgtg aagaaattgc agcctttcta caagaaatcg ctgataaaca tgactatatt
    34681 aaggttatct tgacaggtgc tgggacttct gcttatgtgg gagatacctt gctaccttac
    34741 tttaaggaag tctatgacga acgcaaatgg aatttcaatg ctattgcgac aacagatatc
    34801 gttgccaaac cagcaaccta tttgaaaaaa gatgtggcaa ctgtccttgt gtcttttgct
    34861 cgtagtggga actcgcctga aagtgtggcg actgttgatt tggccaaatc cttggtggat
    34921 gagctttatc aagtgacgat tacttgtgca gcagatggta aattggctct tcaagctcac
    34981 ggtgatgacc gtaatctctt gctcttgcaa ccagctgtct ctaatgatgc tggatttgcc
    35041 atgacttcta gctttacgtc tatgatgttg acaactctct tggtctttga tcctacagaa
    35101 tttgctgtta agtctgaacg ttttgaagtt gtatctagtc ttgcccgtaa agttttagac
    35161 aaggcagaag atgtcaaaga gctcgttgat ttagacttta accgtgtcat ctatctaggc
    35221 gctggtcctt tctttggact tgctcatgaa gctcagctca agattttgga attaactgct
    35281 ggtcaagttg cgaccatgta tgaaagccca gttggcttcc gtcacggtcc aaaatctctt
    35341 atcaacgaca atacagttgt tttggtcttt ggtacaacga cggactacac tcgtaagtac
    35401 gacttggact tggttcgtga agttgctggt gaccagattg ctcgtcgtgt tgtgcttttg
    35461 agtgatcaag cttttggtct tgaaaatgtc aaagaagtgg tccttggttg tggcggtgtc
    35521 ttgaatgata tttaccgtgt cttcccttac atcgtttatg cccaactctt tgccctattg
    35581 acttcactca aggtagaaaa taaaccagat acaccgtctc ctacaggtac agtaaaccgt
    35641 gtagtacaag gtgtcataat tcacgaatat caaaagtaag acagtgttta tgaattcttg
    35701 acaagaggat ttgtaaatta tcagataaac catagattgt cagtacgctt tctatggttt
    35761 gtttgcttga gagaaatagt aaaaggagaa cagaatgaaa gcatacacag agcgtgtatt
    35821 tggaaatgtt gagggtgagg atgtcttggc ctatcgattt gagacagacg gtggctacca
    35881 acttgaggtt atgacttatg gtgcgactat cttgcgctat gtcgcacctg acaaggctgg
    35941 aaattttgcc aatgttatct tgggatttga tgactttgat agttatgtag gcaatagtcc
    36001 caagcatgga gcaagtgtag gtcctgtagc gggtcgtatt gcaggtgcga cctttgagct
    36061 caatggtaag acctatgacc ttgaggttaa taatgctagc aactgtaatc acagtggttc
    36121 aactggttgg gattccagct tgtttgaagt tgaagaagta agcgatcatg gcttgactct
    36181 ctacacagag cgtacagatg ggacaggagg gttccctgga aatctcaaga tttggatcag
    36241 ttatcacttg gaagaaactg gtgcctatga aatcagctac aaggtaacga ccgatcagga
    36301 tacgctggtc aatccaacca accacagcta tttcaacttg tctggtgatt tcacgcagac
    36361 gattgaccgt catgtcttcc aactaaacac agagggcatt tactcaatcg ctcctgacgg
    36421 tgttcctgcc aaaactccag aagccaaccg tgatgtggtc aaacacatct acaatggtgc
    36481 cttgttgaag gatatctttg cagaagaaga tgagcaaatc cagctggcat caggtttgga
    36541 tcatccattt gcccttcctg caggccatga caatgctgga ttcctttatg accaaaattc
    36601 aggtcgcttc ctgcttttca agacagaagc tccttgcttt gtggtctaca cagcaaactt
    36661 tgtggatgaa agtgtcatca taggaggtca gccaatgcta cagcacaatg ggattgctct
    36721 tgaagcgcaa gctttaccag atgccattca cagtgacctt aaaggccaag tcattcttaa
    36781 agctggtcaa accttcacca gtaagacacg ttatgaactt gttgtgaaat aaaaaaagct
    36841 ggtcaaaaag tctttaaaat taaaataacg catattgtca ggtgatgaaa agtttgataa
    36901 tatgcgtttt attatgaaat gatttacttt atattatcct gaaatggtgt tatttttgag
    36961 ttatctggct tgactttctt gatggaaatt atataatagt tgtaacaatg ataattacaa
    37021 ctagaaaaga ggaccattta tgacctatga atacaagagt cacatttatt tggcagagac
    37081 agctttaaat gtaaaggatt tggccagtca aacagccttt tatcagcaag ttattggttt
    37141 agaaatctta tctcaaactg agacagagtc cattctaggc cttggtggaa aagtcttggt
    37201 acagctgatt caagcacaag agagcggaga agtgagggaa tattatggtc tttaccattt
    37261 ggccattctc ttgcccacac gcaaggcttt agctgatgtc ttgaaacacc tgacagattt
    37321 acaaattcct cttgtcggcg gtgcagacca tggttacagt gaagctattt acttggaaga
    37381 tttggaggga aatggcattg aactctatcg agataagcca gtttccacat gggatattcg
    37441 agaagatgga cgcattatcg gagtgacaga ggctcttgcg gcgcaggaca tttatgagtt
    37501 gggggaaaga gtagagccct ttatcctggc agaaggtacg agaatggggc atattcatct
    37561 ttccgtcaag gatagtcgaa agtccagcca gttttatcaa aaggtgttag ggctagagga
    37621 taaattcagt gtgcctagcg ctagttggat tgcggctgga gattaccatc atcatttagc
    37681 agttaacgaa tggggaggaa aaggtctggc ttcgcgtaag caaggcttac caggtttagc
    37741 ctactatgtc atcgaagtcg cacacaaaaa agaactatta acgattgccc aacgagcaca
    37801 agaagttgac gcaccaatca aatggatgac atcgagccaa ttggaaatca cagactcaga
    37861 tggcatcgtg acccgtattc gtttagcaag gtaaaattag gaatttttaa taagttataa
    37921 gtattgctag tttgcttttt gaacggtctt tggtaacaga actgcgtgag ctggatgggc
    37981 gagaattttg acaaagaagc tgattagaga tataatgaag aaaaatcgag caaggaaaaa
    38041 gaaatgacaa attcaaagta tattacttgt ttgaaacgtt cagagggcca gttgtgtggg
    38101 attcaaaaga tgattgaagg agatcgtgac tgtgctgata ttgtgacgca actgacagca
    38161 gtgagatcta gtgtagagcg cgtgattgag atgataatta ccgaaaatct tactgaatgt
    38221 atcaatcagc cactagatga ttctgaagct caaaaggaac gcttagaaaa ggctatccga
    38281 tacttgatta aacggaaata aatgaatgtt ttgagacgat atgtgcaaag gtctattaac
    38341 tattagaata gtttaatggg agtcaaaaga agttgtaaaa aatctttaaa attaaaaaaa
    38401 cgcatagtat caggtattga aatgtactga ccccaaaaag ttagattttt tctgtctaac
    38461 ttttggggtg cagttcataa gaaccttggt aatatgcgtt ttattatggg gagatttata
    38521 cttttcgaaa atctcttcaa accaggtcag cttcaccttg ccgtagatat ggttactgac
    38581 ttcgtcagtt ctatccacaa cctcaaaaca gtgttttgag ctgacttcgt cagttccatc
    38641 cacaacctta aaacagtgtt ttgagctgac ttcgtcagtt ccatccacaa ccttaaaaca
    38701 gtgttttgag cagcctgcgg ctaacttcct agtttgcact ttaattttca ttgagtatta
    38761 cttcatcttc tcatgaaatt gagtttttgc ccagttcctc ttgattattc tacttgacca
    38821 ggccaagcat tcataccacc ttctacgttg gtaacggtga ggccttggga gatgagaaat
    38881 tggcaggtag atgcagagcg gactcctcct tgacaaataa catagtattc atggtcaggt
    38941 ttgagttctt tgtagccttg ctctaaggta cttaatggaa gatttttggc acctggtgca
    39001 tgtcctgctt ggaattcatg tgcttcacgg acatcgataa gttctagatt ttcattttgg
    39061 tattttccat aaaagtcagc catgctgata ctagtttcca tattctactc cttctgggtt
    39121 aaccattttg tatagtgaat aagcgccgtc aaggttttgg gcggtaaatc ctgcttgctt
    39181 gaggataggc tctgcgatat agctgcgcaa accactgtgg cagctgacga tgtaggcttg
    39241 ttttttgtcc agttcatcag cttcctcctt gacactttgt cagtttttga tacaatagta
    39301 caaaattaga ggaggtgggc tatgattcag aaacatgcga ttcctatttt agagtttgat
    39361 gacaatcctc aggcggttat catgcccaat cacgaggggc tggacttgca gttgccaaag
    39421 aagtgtgttt atgcattttt agatgaggag attgaccgct atgcaaggga agtaggggcg
    39481 gattgtgtcg gcgaattcgt ttctgctacc aagacctatc cagtctatgt catcaactac
    39541 aagggtgagg aggtctgtct ggctcaggct cctgttggct ccgctccagc agcccagttt
    39601 atggattggt tgattggcta tggtgtggag cagattatct ctactgggac ctgtggtgtt
    39661 ctagctgata tagaggaaaa tgcctttcta gtccctgttc gcgctctgcg agatgaagga
    39721 gccagttacc actatgtggc accttctcgt tatatggaaa tgcagccaga ggctattgct
    39781 gctattgagg aagttttgga agacagaggg attccttatg aagaagtcat gacctggacg
    39841 acagacggtt tttaccgaga aacggctgaa aaggtggctt atcgtaagga agaaggctgt
    39901 gctgttgtgg agatggagtg ttctgctctt gcggcagtag ctcaattgcg tggggttctc
    39961 tggggtgaat tgttgttcac agcagattct ctagcggact tggaccagta cgacagtcgt
    40021 gactggggct cggaagcttt taataaggcg ctagaactga gtttagcaag tgttcaccac
    40081 ctttagttgt actggcaaag gatttgtttt atcataaaat gtctagctca tacttttcaa
    40141 aaatatgttt aaacgaggtc accttcctct tgtcctaagc atgttgaggt tgggaaaaat
    40201 ctttaaaatc agaaaaacgt atcatatcag gtgatgaaaa ccttgatact atgcgtttta
    40261 tgttggtaag atttagagtg agatgaaatg atactcttcg aaaatctttt caaaccaggt
    40321 cagcttcacc ttgccgtagg tatatgttac tgacttcgtc agtcttatcc ggcaacctca
    40381 aaacggtgtt ttgagctgac ttcgtcagtt ctatttgcaa cctcaaaaca gtgttttgag
    40441 caatctgtga ctagctttct aatcgatgcc ttggttctca ttgcctataa tcaaaaagag
    40501 aaattttctc ctgaaaagca tatagagtag ctggcgttaa aagctcctgt cttgcttttt
    40561 tgacctatag tcacatctat caagtattgt tcttgcctaa gctatcaata aaaaggtggc
    40621 attttttagg cttggtgtta gtagattttg ccttatccta tctaagtcat ttcgaacttt
    40681 ttatggtaca atggaaacat gttattcaaa ttatctaagg aaaaaataga gctaggctta
    40741 tctcgtttat cgccagcccg tcgtattttt ttgagttttg ccttggtcat tttactaggc
    40801 tctcttcttt tgagcttgcc ctttgtccaa gttgaaagct cacgagcgac ttattttgat
    40861 catcttttca ctgctgtctc tgcagtctgt gtgacaggtc tctcaaccct tccagtagct
    40921 cacacctata atatatgggg ccaaataatc tgtttgctct tgattcagat cggtggtcta
    40981 gggctcatga cctttattgg ggttttctat atccagagca agcaaaagct tagtcttcgt
    41041 agccgtgcaa ctattcagga tagttttagt tatggagaaa ctcgatcttt gagaaagttt
    41101 gtctattcta tttttctcac gacctttttg gttgagagct tgggagctat tttgcttagt
    41161 tttcgcctta ttcctcaact tggctgggga cgtggtcttt ttagttccat ttttctagcg
    41221 atctcagcct tctgtaatgc cggttttgat aatttaggga gcaccagttt atttgctttt
    41281 cagaccgatt tactggtcaa tctggtgatt gcaggcttga ttattacagg cggccttggt
    41341 tttatggtct ggtttgattt ggctggtcat gtaggaagaa agaaaaaagg acgtctgcac
    41401 tttcatacga agcttgtact attgttgact ataggtttgt tgttatttgg aacggcaact
    41461 actctctttc ttgagtggaa caatgctgga acgattggca atctccctgt tgccgataag
    41521 gttttagtta gcttttttca aacagtgacg atgcgaacag ctggcttttc tacgatagat
    41581 tatactcagg ctcatcctgt gactcttttg atttatatct tacagatgtt tctaggtggg
    41641 gcacctggag gaacagctgg gggactcaag attacgacat tttttgtcct cttggtcttt
    41701 gcacgaagtg agcttctagg cttgcctcat gccaatgttg cgagacgaac gattgcgccg
    41761 cgaacggttc aaaaatcctt tagtgtcttt attatctttt tgatgagctt cttgatagga
    41821 ttgattctgc tagggataac agccaaaggc aatcctccct ttatccacct catatttgaa
    41881 accatttcag ctcttagtac agttggtgta acggcaaatc tgactcctga ccttgggaaa
    41941 ttggctctca gtgttatcat gccacttatg tttatgggac gaattggtcc cttgaccttg
    42001 tttgttagct tggcagatta ccatccagaa aagaaagata tgattcacta tatgaaagca
    42061 gatattagta ttggttaaga aaggaaagag catgtcagat cgtacgattg gaattttggg
    42121 cttgggaatt tttgggagca gtgtcctagc tgccctagcc aagcaggata tgaatattat
    42181 cgctattgat gaccacgcag agcgcatcaa tcagtttgag ccagttttgg cgcgtggagt
    42241 gattggtgac atcacagatg aagaattatt gagatcagca gggattgata cctgcgatac
    42301 cgttgtagtc gcgacaggtg aaaatctgga gtcgagtgtg cttgcggtta tgcactgtaa
    42361 gagtttgggg gtaccgactg ttattgctaa ggtcaaaagt cagaccgcta agaaagtgct
    42421 agaaaagatt ggagctgact cggttatctc gccagagtat gaaatggggc agtctctagc
    42481 acagaccatt cttttccata atagtgttga tgtctttcag ttggataaaa atgtgtctat
    42541 cgtggagatg aaaattcctc agtcttgggc aggtcaaagt ctgagtaaat tagacctccg
    42601 tggcaaatac aatctgaata ttttgggttt ccgagagcag gaaaattccc cattggatgt
    42661 tgaatttgga ccagatgacc tcttgaaagc agatacctat attttggcag tcatcaacaa
    42721 ccagtatttg gataccctag tagcattgaa ttcgtaaaga gggatgaccc ctcttttttg
    42781 atgcccaaga tggcaaatag agacagaagc cccttgtctt ctagtaaaag ttcttcaaag
    42841 gctggacttt atggtaaaat agaaggaagt gacaagagag agtaatactc aatgaaaatc
    42901 aaagatcaaa ctaggaaact agctgcgggc tgctcaaaac actgttttga ggttgtggat
    42961 agaactgacg aagtcagctc aaaacactgt tttgaggttg cagatagaac tgacgaagtc
    43021 agtaacatct atacggcaag gcgacgttga cgcggtttga agagattttc gaagagtata
    43081 agaaaaaatc agtcccctaa aggagcagat tatgaagtta ttgtctatcg ccatttctag
    43141 ctataatgca gcagcctatc ttcattactg tgtggagtcg ctagtgattg gtggtgagca
    43201 agttgggatt ttgattatca atgacgggtc tcaggatcag actcaggaaa tcgctgagtg
    43261 tttagctagc aagtatccta atatcgttag agccatctat caggaaaata aatgccatgg
    43321 cggtgcggtc aatcgtggct tggcagaggc ttctgggcgc tattttaaag tagttgacag
    43381 tgatgactgg gtggatcctc gtgcctactt gaaaattctt gaaaccttgc aggaacttga
    43441 gagcaagggt caagaggtgg atgcctttgt gaccaatttt gtctatgaaa aggaaggtca
    43501 gtctcgtaag aagagtatga gttacgagtc agtcttgcct gttcggcaga tttttgacta
    43561 ggaccaggtc ggaaatttct ccaaaggcca gtatatcatg atacactcgc tgatttatcg
    43621 gacagatttg ttgcgtgcta gccagttcta actgcctgaa catacttttt atgtcgatga
    43681 tctctttgtc tttacgcccc ttcagcaggt caagaccatg tactatctgc ctgtcgattt
    43741 ctatcgttat ttgattgggc gtgaggacca gtctgtcaat gagcaagtga tgattaagcg
    43801 cattgaccag caactcaagg tcaatcgact cttggtagac cagcttgatt tgtcccaagt
    43861 gagtcatcct aaaatgcgag aatatctgct gaatcatatt gaaatcacga cggtgatttc
    43921 cagtaccctg ctcaaccgat ctgaaacagc ggagcatctg gcaaaaaaac gccaattgtg
    43981 gacctatatt cagcagaaaa atccagaagt ctttcaggct attcgtaaga ccatgttgag
    44041 ccgtttgacc aaacattctg tcttgccaga tcgcaaactg tccaatgtcg tctatcaaat
    44101 caccaaatct gtttatggat ttaattaata taagtgtttt ataagaggga tttaagaaaa
    44161 attttaactt tttcttagtc ctttttaatt tcaggagatt atactagagt catcaaataa
    44221 agaaagactc taaggagaat cctatgaaat tcaatccaaa tcaaagatat actcgttggt
    44281 ctattcgccg tctcagtgtc ggtgttgcct cagttgttgt ggctagtggc ttctttgtcc
    44341 tagttggtca gccaagttct gtacgtgccg atgtggtcaa tccgacccca ggtcaagtct
    44401 tacctgaaga gacatcggga acgaaagagg gtgacttatc agaaaaacca ggagacaccg
    44461 ttctcactca agcgaaacct gagggcgtta ctggaaatac gaattcactt ccgacaccta
    44521 cagaaagaac tgaagtgagc gaggaaacaa actcttctag tctggataca ctttttgaaa
    44581 aagatgaaga agctcaaaaa aatccagagc taacagatgt cttaaaagaa actgtagata
    44641 cagctgatgt ggatgggaca caagcaagtc cagcagaaac tactcctgaa caagtaaaag
    44701 gtggagtgaa agaaaataca aaagacagca tcgatgttcc tgctgcttat cttgaaaaag
    44761 ctgaagggaa aggtcctttc actgccggtg taaaccaagt aattccttat gaactattcg
    44821 ctggtgatgg tatgttaact cgtctattac taaaagcttc ggataatgct ccttggtctg
    44881 acaatggtac tgctaaaaat cctgctttac ctcctcttga aggattaaca aaagggaaat
    44941 acttctatga agtagactta aatggcaata ctgttggtaa acaaggtcaa gctttaattg
    45001 atcaacttcg cgctaatggt actcaaactt ataaagctac tgttaaagtt tacggaaata
    45061 aagacggtaa agctgacttg actaatctag ttgctactaa aaatgtagac atcaacatca
    45121 atggattagt tgctaaagaa actgtcgaaa aagctgtta
//
LOCUS       DAVBCB010000011        42049 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVBCB010000011 DAVBCB010000000
VERSION     DAVBCB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 42049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42049
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.011"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..648
                     /locus_tag="VQU28_000578"
                     /pseudo
     CDS             1..648
                     /locus_tag="VQU28_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(693..1334)
                     /gene="plsY"
                     /locus_tag="VQU28_000579"
     CDS             complement(693..1334)
                     /gene="plsY"
                     /locus_tag="VQU28_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HET2475983.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1470..3413
                     /gene="parE"
                     /locus_tag="VQU28_000580"
     CDS             1470..3413
                     /gene="parE"
                     /locus_tag="VQU28_000580"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HET2475984.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            3833..6304
                     /gene="parC"
                     /locus_tag="VQU28_000581"
     CDS             3833..6304
                     /gene="parC"
                     /locus_tag="VQU28_000581"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HET2475985.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            6436..7458
                     /locus_tag="VQU28_000582"
     CDS             6436..7458
                     /locus_tag="VQU28_000582"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HET2475986.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7514..8218
                     /locus_tag="VQU28_000583"
     CDS             7514..8218
                     /locus_tag="VQU28_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2475987.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSDM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            8283..8972
                     /locus_tag="VQU28_000584"
     CDS             8283..8972
                     /locus_tag="VQU28_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HET2475988.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            8969..9892
                     /locus_tag="VQU28_000585"
     CDS             8969..9892
                     /locus_tag="VQU28_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HET2475989.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            9907..10551
                     /gene="pcp"
                     /locus_tag="VQU28_000586"
     CDS             9907..10551
                     /gene="pcp"
                     /locus_tag="VQU28_000586"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2475990.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            10719..10949
                     /locus_tag="VQU28_000587"
     CDS             10719..10949
                     /locus_tag="VQU28_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HET2475991.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            10999..11079
                     /locus_tag="VQU28_000588"
     tRNA            10999..11079
                     /locus_tag="VQU28_000588"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11033..11035,aa:Tyr,seq:gta)
     gene            11089..11160
                     /locus_tag="VQU28_000589"
     tRNA            11089..11160
                     /locus_tag="VQU28_000589"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11121..11123,aa:Gln,seq:ttg)
     gene            11322..12524
                     /gene="rpsA"
                     /locus_tag="VQU28_000590"
     CDS             11322..12524
                     /gene="rpsA"
                     /locus_tag="VQU28_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HET2475992.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            12610..13107
                     /locus_tag="VQU28_000591"
     CDS             12610..13107
                     /locus_tag="VQU28_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HET2475993.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            13107..14762
                     /gene="dnaX"
                     /locus_tag="VQU28_000592"
     CDS             13107..14762
                     /gene="dnaX"
                     /locus_tag="VQU28_000592"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HET2475994.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            14790..14984
                     /locus_tag="VQU28_000593"
     CDS             14790..14984
                     /locus_tag="VQU28_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HET2475995.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            15134..15904
                     /gene="sufC"
                     /locus_tag="VQU28_000594"
     CDS             15134..15904
                     /gene="sufC"
                     /locus_tag="VQU28_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HET2475996.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITRLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            15932..17194
                     /gene="sufD"
                     /locus_tag="VQU28_000595"
     CDS             15932..17194
                     /gene="sufD"
                     /locus_tag="VQU28_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HET2475997.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSYADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            17205..18431
                     /locus_tag="VQU28_000596"
     CDS             17205..18431
                     /locus_tag="VQU28_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2475998.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPASARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            18418..18858
                     /locus_tag="VQU28_000597"
     CDS             18418..18858
                     /locus_tag="VQU28_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HET2475999.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            18908..20320
                     /gene="sufB"
                     /locus_tag="VQU28_000598"
     CDS             18908..20320
                     /gene="sufB"
                     /locus_tag="VQU28_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HET2476000.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(20551..21792)
                     /locus_tag="VQU28_000599"
     CDS             complement(20551..21792)
                     /locus_tag="VQU28_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HET2476001.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITAYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFIDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            21895..23004
                     /locus_tag="VQU28_000600"
     CDS             21895..23004
                     /locus_tag="VQU28_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2476002.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            23538..23885
                     /gene="ssrA"
                     /locus_tag="VQU28_000601"
     tmRNA           23538..23885
                     /gene="ssrA"
                     /locus_tag="VQU28_000601"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24068..24155)
                     /locus_tag="VQU28_000602"
     tRNA            complement(24068..24155)
                     /locus_tag="VQU28_000602"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24119..24121),aa:Ser,seq:gga)
     gene            24317..25057
                     /locus_tag="VQU28_000603"
     CDS             24317..25057
                     /locus_tag="VQU28_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2476003.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNITVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            25054..25965
                     /gene="pfkB"
                     /locus_tag="VQU28_000604"
     CDS             25054..25965
                     /gene="pfkB"
                     /locus_tag="VQU28_000604"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HET2476004.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVESVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDVVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            25962..27914
                     /locus_tag="VQU28_000605"
     CDS             25962..27914
                     /locus_tag="VQU28_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2476005.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGALYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            28078..30303
                     /locus_tag="VQU28_000606"
     CDS             28078..30303
                     /locus_tag="VQU28_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HET2476006.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(30348..30776)
                     /locus_tag="VQU28_000607"
     CDS             complement(30348..30776)
                     /locus_tag="VQU28_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476007.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITASITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            30914..32056
                     /locus_tag="VQU28_000608"
     CDS             30914..32056
                     /locus_tag="VQU28_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2476008.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     NYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            32065..33279
                     /gene="thiI"
                     /locus_tag="VQU28_000609"
     CDS             32065..33279
                     /gene="thiI"
                     /locus_tag="VQU28_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HET2476009.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            33414..34238
                     /locus_tag="VQU28_000610"
     CDS             33414..34238
                     /locus_tag="VQU28_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2476010.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRIFIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            34260..34999
                     /locus_tag="VQU28_000611"
                     /pseudo
     CDS             34260..34999
                     /locus_tag="VQU28_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            35012..36178
                     /locus_tag="VQU28_000612"
     CDS             35012..36178
                     /locus_tag="VQU28_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2476011.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            36686..38179
                     /locus_tag="VQU28_000613"
     CDS             36686..38179
                     /locus_tag="VQU28_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HET2476012.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            38192..39238
                     /locus_tag="VQU28_000614"
     CDS             38192..39238
                     /locus_tag="VQU28_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2476013.1"
                     /translation="MKKVKLGEVLSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLREHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLLVKSRFNEMFGDVILNEKEWKVSKWNEILTIRNGKNQKQVEDADGKFPIY
                     GSGGIMGYAKDWIVKKNSVIIGRKGNINKPILVRENFWNVDTAFGLEPVLEKINSEYL
                     FYFCQLYNFEKLNKAVTIPSLTKSDLLNISIPLPPLALQNEFADFVALVDKSQFACEI
                     AIKVWRNSLKFSII"
     gene            39268..41964
                     /locus_tag="VQU28_000615"
                     /pseudo
     CDS             39268..41964
                     /locus_tag="VQU28_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
BASE COUNT        12769 a         7563 c         9309 g        12408 t
ORIGIN      
        1 cgtgcttatc tggtaaatca taaaagagtt caaggcttga tgaaagtact caatttacaa
       61 gctaaaatgc gacagaaacg aaaatattct tctcataaag gagacgttgg caagaaggca
      121 gagaatctca ttcaaggcca atttgaaggc tctaaaacaa tggaaaagtg ctacacagat
      181 gtgacagaat ttgccattcc agcaagtact caaaagcttt acttatcacc agttttagat
      241 ggctttaaca gcgaaattat cgcctataat ctttcaactt cgcccaactt agaacaagta
      301 taaacaatgt tggaacaggc attcaaagag aagcactacg agaatacgat tctccatagt
      361 gaccaaggct ggcaatatca acacgattct tatcatcagt tcctagagag taagggaatt
      421 caagcatcca tgtcacgtaa gggaaacagc ccagacaatg gtatgatgga gtccttcttt
      481 gggattctga aatcggaaat gttttacggt tatgagaagt cgtttcagtc gcttaagcaa
      541 ttggaacaag ccattataga ctatattgat tactacaaca ataaacgaat taagataaaa
      601 ctaaaaggac ttagccctgt gcaatacaga actaaatcct tcggataaat taattgtcta
      661 acttttgggg tgcagttcac agaactggca ttttattttt taggatcttg atgggttagg
      721 ttcaatcccc aagggaccaa attttcagtt ttatttttga tacgagctat attgtcctta
      781 tgacgaatga taatcaaact agcaagtgct aagataatag cgatgaagag agagtcatag
      841 ttactcagga taaaaccaaa aagtggaaag agcagaaccc cgataacagc cgcgatcgat
      901 gctgtgacac tagacagtga aatcatactg ccaagataga gagctccaaa gaagataatc
      961 gcaaggtaga gacagaagat aggcgcaaat ccgaaaatta ctccagcact ggttgcgaca
     1021 gccttaccac ctttaaatcc tgcaaagata gggaaggtat ggccgataac agccaaaagt
     1081 ccaaagatga gaggagaaac gccttgtaga tgaaaaataa tcggaagcag cgttgctagg
     1141 gttcctttga aaaagtcaat cacaaaggtt gccataccag ctttcttacc taaaatgcgg
     1201 aaggtattgg tcgttccagt gttaccagaa ccatgctcgc gtagattgat ttgaaagaat
     1261 acttgtccaa tccagagacc agatggaatc gaacccagca gataggctag gattaataaa
     1321 actattgtaa tcatactcct attatatcat gaattgggaa agaaaagcag agaatctctg
     1381 ctaaaattgt cacatcggag aaagaaaaat tttgcaaaat ccttggaaaa cctgtagaat
     1441 agtaaagatg aacgaatagg aggttccttg tgtcaaaaaa ggaaatcaat attaacaatt
     1501 ataatgatga tgctattcag gtgctagaag ggttggatgc ggtccgaaaa cgtccaggga
     1561 tgtatattgg atcgaccgat ggcgctggtc ttcatcacct agtttgggaa atcgttgata
     1621 atgcagtcga tgaagccttg tctgggtttg gtgatcgtat cgatgtaacc atcaataaag
     1681 acggtagtct aacggttcaa gaccatggac gtgggatgcc gacaggtatg cacgctatgg
     1741 gaattccaac tgttgaggtt atctttacca ttcttcatgc cggagggaaa ttcggtcaag
     1801 gtggctataa gacatcaggt ggacttcacg gagtgggttc ttccgttgtt aacgcccttt
     1861 ctagctggtt agaagttgaa attacccgtg atggcgcagt ttacaagcaa cgtttcgaaa
     1921 atggtggaaa acctgtcacg actttgaaga aaatcggtac agcacccaag tctaaaacag
     1981 gcaccaaagt tacttttatg cctgacgcga ctatattttc tacgacagat ttcaagtaca
     2041 ataccatttc agagcgcctt aatgaatcag cctttctctt gaaaaatgtg accttgtctt
     2101 taacggacaa gcgaacagat gaagcgattg agttccacta tgagaatgga gtacaagatt
     2161 ttgtttctta tctcaacgaa gataaggaaa tcttgacgcc agttctttac tttgaagggg
     2221 aagacaatgg ttttcaagtg gaagtagccc tccagtacaa tgacggattc tcagataaca
     2281 ttctatcctt tgtcaataac gttcgcacca aggacggtgg aacgcacgag acaggactca
     2341 agtctgccat taccaaggtc atgaatgact atgcacgtaa aacaggtctt ctcaaggaaa
     2401 aagataaaaa ccttgaaggt tcagactatc gtgagggact agcggccgtt ctttctatct
     2461 tagttcctga agaacacttg cagtttgaag gacagaccaa ggataaacta ggaagccccc
     2521 tagctcgccc agttgtggat ggaatagtgg ctgataagtt gacctttttc cttatggaaa
     2581 atggggaatt agcttctaac ctcatccgca aggctatcaa ggcccgtgat gctcgtgaag
     2641 cagcacgtaa ggcgcgtgat gagagccgaa atgggaagaa aaacaagaaa gataagggct
     2701 tgttgtctgg gaaattgacc ccagcccaat ctaagaatcc tgctaagaat gaactctatc
     2761 tagttgaggg ggactctgcc ggtggttctg ccaaacaagg tcgtgaccgc aagttccagg
     2821 ctattctacc tcttcgtggt aaggttatca atacagccaa ggccaagatg gcggatatcc
     2881 tcaaaaatga agagatcaat accatgattt ataccattgg tgcgggtgtt ggagcagact
     2941 tctctattga agatgccaac tatgataaga tcattatcat gaccgatgcg gataccgacg
     3001 gtgcccatat ccagaccttg ctcttgacat ttttctaccg ttacatgcgt ccgctagtcg
     3061 aggcaggtca tgtctatatt gccctcccac ctctttacaa gatgtccaaa ggtaaaggaa
     3121 agaaagaaga agtggcctac gcttggacgg acggagaact agaagaactc cgtaaacagt
     3181 tcggtaaagg cgctaccctc caacgataca aaggacttgg tgagatgaat gcggaccagc
     3241 tctgggaaac aaccatgaac ccagaaacac gtaccctcat ccgtgtcaca attgaagatt
     3301 tagcgcgcgc cgaacgccgc gtcaatgttc tcatgggaga taaggtagaa ccacgccgta
     3361 aatggattga agataatgtc aagtttacgc tagaagaagc gacagtgttt taatgaaaga
     3421 aaataatatg ttagtaaact ggaaattaga gtcggatgtg aatgactatg tgaaaaagca
     3481 gtttgaaaat cttggtttga aaaaattgca ggattattaa tgaagaatct gctatgagtg
     3541 cttatctcaa agaggcgctt aagggagcgg ctaagacaca aactaagact aattttggaa
     3601 aaccagattt tcatattgaa aaatacaaac aggatagttt ggttacttat cgtaactgaa
     3661 cacgggagga attcccgtaa aatatctttt actgcataaa aacctactct acattctttg
     3721 aaaggagttg aacacgccct agatactgtg tgaaaaagat aaattctctt gtgagtttgc
     3781 ttacttcaag aattttctat tttcacttgg tattttatgg gctttgtatc ttatgtctaa
     3841 cattcaaaac atgtccctgg aggacatcat gggagagcgc tttggtcgct actccaagta
     3901 cattattcaa gaccgggctt tgccagatat tcgtgatggg ttgaagccgg ttcagcgccg
     3961 tattctttat tctatgaata aggatagcaa tacttttgac aagagctacc gtaagtcggc
     4021 caagtcagtc gggaacatca tggggaattt ccacccacac ggggattctt ctatctatga
     4081 tgccatggtt cgtatgtcac agaactggaa aaatcgtgag attctagttg aaatgcacgg
     4141 taataacggt tctatggacg gagatcctcc tgcggctatg cgttatactg aggcacgttt
     4201 gtctgaaatt gcaggttacc ttcttcagga tatcgagaaa aatacagttc cttttgcatg
     4261 gaactttgac gatacggaga aagaaccaac ggtcttgcca gcagcctttc caaacctctt
     4321 ggtcaatggt tcgactggga tttcggctgg ttatgccaca gacattcctc cccataattt
     4381 agctgaggtc atagatgctg cagtttacat gattgaccac ccaactgcaa agattgataa
     4441 actcatggaa ttcttgcctg gaccagactt ccctacaggg gctattattc agggtcgtga
     4501 tgaaatcaag aaagcttatg agactgggaa agggcgcgtg gttgttcgtt ccaagactga
     4561 aattgaaaag ctaaaaggtg gtaaggaaca aatcgttatt actgagattc cttatgaaat
     4621 caataaggcc aatctagtca agaaaatcga tgatgttcgt gttaataaca aggtagctgg
     4681 gattgctgag gttcgtgatg agtctgaccg tgatggtctt cgtatcgcta tcgaacttaa
     4741 gaaagacgct aatactgagc ttgttctcaa ctacttattt aagtacaccg acctacaaat
     4801 caactacaac tttaatatgg tggcgattga caatttcaca cctcgtcagg ttgggattgt
     4861 tccaatcctg tctagctaca tcgctcaccg tcgagaagtg attttggcgc gttcacgctt
     4921 tgacaaagaa aaggctgaga aacgtctcca tatcgtcgaa ggtttgattc gtgtgatttc
     4981 gattttggat gaagtcattg ctcttatccg tgcttctgag aataaggcgg acgccaagga
     5041 aaacctcaaa gttagctatg attttacgga agaacaggct gaggctatcg taactttgca
     5101 actgtaccgt ttgaccaata ccgatgtggt tgtcttgcag gaagaagaag cagagcttcg
     5161 tgagaagatt gctatgctgg cggctattat cggtgatgaa aggactatgt acaatctcat
     5221 gaagaaagaa cttcgtgagg tcaagaagaa atttgcaact cctcgtttga gttctttaga
     5281 agacactgcg aaagcaattg agattgatac agctagtctt atcgctgagg aagataccta
     5341 cgtcagcgtg accaaggcag gttacatcaa gcgtaccagt ccacgttcct ttgcggcttc
     5401 caccttggaa gaaattggca agcgtgatga tgaccgtttg atttttgttc aatctgccaa
     5461 gacaacccag cacctcttga tgttcacaag tcttggaaat gtcatctaca gaccaatcca
     5521 tgagttggca gatattcgtt ggaaggacat cggagagcat ctgagccaaa ccatcacaaa
     5581 ctttgaaacg aatgaagaaa tcctttatgt ggaagtactg gatcagtttg acgatgcgac
     5641 aacctacttt gcagtgactc gccttggtca aatcaaacgg gtagagcgaa aagaattcac
     5701 tccatggcgg acctatagat ctaagtctgt caagtatgct aagctcaaag acgatacaga
     5761 tcagattgta gcagtggctc cgattaaact agatgatgtt gtcttggtta gtcaaaatgg
     5821 ttatgccctg cgtttcaata tcgaagaggt tccggttgtc ggtgctaagg cagcaggtgt
     5881 caaggctatg aatttgaaag aagatgatgt cctccaatct ggctttatct gtaatacttc
     5941 gtccttctac ctcttgaccc agcgtggaag cttgaaacgt gtttctattg aggaaattct
     6001 agcaaccagc cgtgccaaac gaggattaca agtcttgcgt gagttgaaaa acaaaccgca
     6061 tcgtgtcttc ttggcaggag cagttgcaga gcaaggattt gttggcgatt tcttcagtac
     6121 ggaagtggat gtgaacgacc aaactctgct tgtccaatcc aataaaggaa caatctatga
     6181 aagccgattg caagacttga acttgtcaga acgcactagc aatggaagct tcatttctga
     6241 cacgatttca gatgaagaag tttttgacgc ttatcttcag gaagtagtta ctgaagataa
     6301 ataagtgaaa tgaagaatca gttctaagag ctggttttta ttggagaaat tttctgaaaa
     6361 ttacaaaata tacttgctat tttagaaaaa tagtgtagaa tataagaaat aggtttttag
     6421 aataaggagt ggaatatgac agtaacgatt gattgggaaa acctcggttt ttcctatatg
     6481 aaattacctt atcgctatct tgctcatttc aaaaatggac aatgggatca aggagagctt
     6541 acagaggatg caactttgca tatttcagag tcttctccaa gtcttcacta tggacaacaa
     6601 gcatttgaag gtttgaaagc ttatcgtact aaggatggca gtgttcaact gttccgtcct
     6661 gatgaaaatg ctaaacgtct gcaacgtaca tgtgaccgtc tcttgatgcc acaagttccg
     6721 acagacatgt ttgtagaagc ttgtaaagca gttgtccgtg cgaatgaaga atacgtacca
     6781 ccatacggaa caggtggaac cttatatctt cgccctcttt tgattggtgt cggagatatt
     6841 atcggggtaa aaccggcaga agagtacatt ttcaccatct ttgctatgcc agttggaaat
     6901 tactttaaag gtggtttggt cccaaccaac ttcttgattc aggatgagta cgaccgtgca
     6961 gcaccaaatg gtacaggtgc ggctaaggtt ggtggaaact atgctgcaag tctcttacca
     7021 ggaaaaatgg ccaagtcacg tcatttctca gatgttatct atctggaccc atcaactcat
     7081 acaaagattg aagaagtcgg atcagctaac ttctttggaa ttacagctga taatgaattt
     7141 gtaacaccat tgagtccatc tatcttgcca tctattacca agtattcctt gctttatttg
     7201 gcagaacatc gcttgggatt aactcctatt gagggtgatg ttccaattga taatcttgac
     7261 cgttttgtag aggcaggtgc ctgtggtaca gcagcggtta tttctccaat tggaggtatt
     7321 caacatggtg atgatttcca tgtattctat agtgaaacag aagtaggtcc tgtgacgcgt
     7381 aaattatata atgaattgac gggtattcag tttggcgata ttgaagcgcc agaaggttgg
     7441 attgtaaaag tagattaaaa taaaccaaag gagatttttt atgaaataga aaaagtggct
     7501 cttaacagca ggagtggtcc tgagcacgtc agctatttta gtggcttgtg gaaaaactga
     7561 taaagaagca gatgcaccga caacattttc ttatgtctat gcagtagatc cagcatcatt
     7621 gggctacagt atagcgactc gaacatcgag gacagacgtt attggaaatg ttattgatgg
     7681 tttgatggaa aatgataaat acggcaatgt tgctccttct caaaaagact atgatttgaa
     7741 cagtacagga tgggctccaa gctatcaaga tccagcgtct tacttgaata ttatggatcc
     7801 aaaatctggt tctgacatga aacaccttgg cattacgaaa ggaaaagata aggatgttgt
     7861 agctaaacct ggtttggata aatataagaa attgttagaa gatgctgttt ctgagaccac
     7921 tgacctagag aagagatatg aaaaatatgc caaagctcaa gcttggtcga cagatagttc
     7981 attattgatg ccaacagctt catctggtgg ttttccagtt gtaagtaacg tagtaccatt
     8041 ctcaaaacca tactcacaag ttggtattaa gggggaacca tatatcttta aaggaatgaa
     8101 attgcaaaaa gatattgtta caacaaaaga atataacgag gtttttaaaa aatggcaaaa
     8161 agaaaaattg gaatccaata gcaaatacca aaaagaacta gaaaaataca ttaaataagg
     8221 aatggtattg atcttgataa aatttttaaa atactgtcat tttgaatata aaggagtttg
     8281 atatggagtg gattagatta ataggaatag caatcattgt tgtgggtttt attttaaaat
     8341 ttgatacaat tgcaacagta gtcttagctg gtttggttac agctttagtt tcaggtgttt
     8401 ctctcgttga atttttggag attttgggaa aagaatttag caatcagcga gtgctcacga
     8461 tttttatggt taccttgcct cttgtggggc tgtcagaaac ctttggactc aagcaacgat
     8521 caatcgattt gattcgaaag attaaaggtc tgacagttgg aaacttctat acagtttatt
     8581 tctttattcg agagttagct ggtttctttt caattcgtct aggaggacac cctcagtttg
     8641 tcagaccttt ggttcaacct atgggagaag cagctgcaga gtctcaatta ggtagaaagt
     8701 taacagaggt tgaagatgag acaataaaag cgcgtgcggc tgcgaatgaa aattttggaa
     8761 atttctttgc tcaaaatacg tttgtaggtg ctgggggagt cctcttgata gggggaacat
     8821 tagatcagtt aggctatgaa agcaattatg cagggattgc ttctacatct attattgttg
     8881 ctgttataac acttattgta gtggggattt acaattattt atttgataaa aaattgatat
     8941 caaaaaagac taggggagga gaacaaaaat gacagagttg gcaaagcaac tattagagtt
     9001 gacctatatt gtgattggtt gtcaatttct ccatacagcc tattgtagtt ataaagataa
     9061 aacaaaccca gttcgacttg ggacatctgc attttggact ctattgtcta ttacgtttat
     9121 aggtggttcc tatatgccaa atatgagtat tggtattatt gtaatcctat tatcgctgtt
     9181 aacattgttt aagcaagtcc gtatcggaac cttgccatcc ttagatgaaa tgaaagccaa
     9241 tattgaatct aacaggttga aaaataaaat ttttattcca gttatgctga tggcaatact
     9301 tgcgttggtc ttagcgcaaa tgattccaga atttagcaag atttcgatta gccttgccgc
     9361 cttgtttgct acaatttctg ttcttgtgat taccaatagt caccctaaga gtctgttatc
     9421 agaaaataat cgaatgactc agcaagtttc aacaagtggg attgttcctc aattattagg
     9481 ggctttgggg gctattttta ctgtagcagg tgttggtgat gttatctctc atctgattag
     9541 cggtattgtt ccttcagata gtcgctttat aggagttttg gcttatgttc ttggaatggt
     9601 tctattcaca atgattatgg gaaatgcttt tgcagcattc accgttatta cagcaggtgt
     9661 tggagttccc tttgtatttg ctctgggagc taatccaatt gtggctggtg ctcttgccat
     9721 gacagcaggt tattgtggga ccttattgac cccaatggct gctaatttta acgctctacc
     9781 agcagcattg atggatatga aagatcagaa tggcgttata aaggctcaag caggtgttgc
     9841 tctagtaatg attgttattc acatattctt aatgtacttt ctcgcatttt agtaaaggaa
     9901 aataaaatga aaatattggt tacaggtttt aatccttttg gaggtgaaaa gattaatcca
     9961 gctttggagg ctgtaaaatt attaccatct gagattaatg gggctgaagt tcgctgggta
    10021 gaaattccaa cggtttttta taagtcgtca gaagttttag aggcagaaat attacgatat
    10081 caaccagatg ctgtactttg tattggacaa gcaggcggca ggaccggctt aacacctgaa
    10141 cgagtggcta ttaatcaaga tgatgctcgc atacctgata acgaaggcaa tcaaccaatt
    10201 gatacaccga ttcgtattga tggagcatcg gcctatttta gtagtttacc tatcaaagcg
    10261 atggtacaag ctatcaaaaa acagggactt ccggcagttg tatccaatag tgcaggaacc
    10321 tttgtttgca atcatttgat gtaccaagct ctttatttag tggataaaaa attccctaat
    10381 atgagagcag gtttcatgca tattccatat atgatggaac aagtagtaaa taagccgaat
    10441 actgcaggaa tgagtctatg tgatattgta agaggcatag aggtagctat tgaagctatt
    10501 gtagactaca aagataagga tttgcagtta gtaggcggtg agactcactg ataacctaaa
    10561 aaggatagtc aattatgctt gtttactaac tattaactat gctaaatcaa ttgaggttgt
    10621 ttacataaaa ctctatatca gagaagcctg atatagagtt ttttcttgct agttttagga
    10681 ttttcttgta aaatagaaaa agtgaagaga ggtatgaaat gagcaagaaa gataaaaaaa
    10741 tcgaaattca agtagcggat gccaaagtta atgttggtaa agacagtttt gaaggttata
    10801 cattgactat cggtaaaaaa gttatcggag aaattgccga attagacgga caatttgcca
    10861 ttataaagaa tgggaatgtc gatagttttt ataaaaaatt ggaaaaagct gtggaaattt
    10921 tgattgaaaa ttataattta gcaaaataag tcttgttttt ttgaaatttt catgatataa
    10981 tagtccatgt tgattgtagg agagatagcg aagaggctaa acgcggcgga ctgtaaatcc
    11041 gctccttcgg gttcgggggt tcgaatccct ctctctccat ttcattaatg gggtatagcc
    11101 aagcggtaag gcaagggact ttgactccct catgcgttgg ttcgaatcca gctaccccag
    11161 ttcttaggta ataatcaaga taaaaagcaa aatatcttag ggtattttat ttttataatt
    11221 gaaagacgtg aatgatatga acatgtcctt gcgggtgctt aggaaaaaaa ttataagtat
    11281 gtcaagttta agaaaaactt gattgttgga ggatttttta gatgaacgaa tttgaagatt
    11341 tgctaaatag cgttagtcaa gttgagactg gtgatgttgt tagtgctgaa gtattgacag
    11401 ttgatgcgac tcaagctaac gttgcaatct ctggaactgg tgttgaaggt gtcttgactc
    11461 ttcgcgaatt gacaaacgat cgtgatgcag atatcaatga ctttgttaaa gtaggagaag
    11521 tattggatgt tcttgtactt cgtcaagtag ttggtaaaga tactgataca gttacatacc
    11581 ttgtatctaa aaaacgcctt gaagctcgca aagcatggga caaacttgtt ggtcgcgaag
    11641 aagaagttgt tactgttaaa ggaacgcgtg ccgttaaagg tggactttca gtagaatttg
    11701 aaggtgttcg tggatttatc ccagcttcaa tgttggatac tcgtttcgta cgtaacgctg
    11761 agcgttttgt aggtcaagaa tttgatacta aaatcaaaga agttaacgct aaagaaaacc
    11821 gcttcatcct ttcacgtcgt gaagttgttg aagcagctac tgcagcagct cgcgctgaag
    11881 tattcggtaa attggctgtt ggtgatgttg taactggtaa agttgctcgt atcacaagct
    11941 tcggcgcttt catcgacctt ggtggtgttg acggattggt tcacttgact gaattgtcac
    12001 atgaacgtaa cgtatcacca aaatcagttg taactgttgg tgaagaaatt gaagtgaaaa
    12061 tccttgatct taacgaagaa gaaggacgtg tatcactttc acttaaagca acagtaccag
    12121 gaccatggga tggcgttgag caaaaattgg ctaaaggtga tgtagtagaa ggaacagtta
    12181 aacgtttgac tgacttcggt gcatttgttg aagtattgcc aggtatcgat ggacttgttc
    12241 acgtatcaca aatttcacac aaacggattg aaaatccaaa agaagctctt aaagttggtc
    12301 aagaagttca agttaaagtt cttgaagtta acgcagatgc agaacgcgtg tcactttcta
    12361 ttaaagctct tgaagaacgt ccagcccaag aagaaggaca aaaagaagaa aaacgtgctg
    12421 ctcgtccacg tcgtccaaga cgtcaagaaa agcgtgattt cgaacttcca gaaacacaaa
    12481 caggattttc aatggctgat ttgtttggtg atatcgaact ttaatcaaat tgaaaattca
    12541 caaaatcctt tgtttactaa acaagggatt tttcttttgt ctctgctcct tttttgatat
    12601 aatagttcta tgttaaaatc agaaaaacaa tcacgttatc aaatgttaaa tgaagaattg
    12661 tccttcctat tggaaggcga aaccaatgtt ttggctaatc tttccaacgc cagtgctctt
    12721 ataaaatcac gttttcctaa taccgtattt gcaggctttt atttgttcga tggaaaggaa
    12781 ttggttttag gtcccttcca aggaggtgtt tcctgcatcc gtattgcact aggcaagggt
    12841 gtttgtggtg aggcagctca ctttcaggaa actgttattg ttggagatgt gacgacctat
    12901 ctcaactata tttcttgtga tagtctagct aaaagtgaaa ttgtggtgcc gatgatgaag
    12961 aatggtcagt tacttggagt tctggatctg gattcttcag agattgagga ttacgatgct
    13021 atggatcgag attatttgga acaatttgtc gctattttgc ttgaaaagac aacatgggac
    13081 tttacgatgt ttgaggaaaa atcttaatgt atcaagcact ttatcgaaaa tatagaagtc
    13141 aaaacttctc ccagttagtt ggtcaagaag ttgtggctaa gactcttaaa caagcggtgg
    13201 agcaagagaa aataagtcac gcttatcttt tttctggtcc tcgtggaacg ggaaaaacca
    13261 gtgttgctaa aatctttgcc aaggctatga actgtcccaa tcaagtgggt ggcgaacctt
    13321 gcaataactg ctatatttgt caagcagtga cggacggtag tttagaagat gtcattgaaa
    13381 tggatgcagc ttctaataat ggggtagatg aaattcgcga aattcgtgat aaatctacct
    13441 atgcgcctag ccttgctcgt tataaggttt atatcataga tgaggttcac atgctgtcta
    13501 caggggcttt taatgccctc ctaaagacgc tggaagaacc aacacagaat gtagtcttta
    13561 ttttggccac tactgaatta cacaagattc ctgctactat tctatcccgt gtgcaacgtt
    13621 ttgagtttaa atcaattaag acacaggata ttaaggaaca tattcactat atcttagaaa
    13681 aagaaaatat cagttctgaa ccagaggctg tggaaatcat tgccagacgg gcggaaggtg
    13741 gaatgcggga cgccttgtct attttggatc aagccctgag tttgacacag ggaaatgagc
    13801 tgacgactgc tatctctgaa gaaattactg gcactattag cctatcagcc ttggatgatt
    13861 atgtggcggc cttgtctcaa caggatgttc ccaaagcttt gtcttgcttg aatcttcttt
    13921 ttgacaatgg taagagcatg actcgttttg tgaccgatct tttgcactat ttaagagact
    13981 tgttaattgt tcaaacaggg ggagaaaata ctcatcatag ttcagtcttt gtagaaaatt
    14041 tggcacttcc tcaaaaaaat ctgtttgaaa tgattcgctt agcaacagtg agtttagcag
    14101 atattaagtc tagtttgcag cccaagattt atgctgaaat gatgaccgtc cgtttggcgg
    14161 aaatcaagcc cgaaccagct ctatcaggag cggttgaaaa tgaaattgct acgctgagac
    14221 aggaagttgc ccgtctcaaa caagagcttt ctaatgtagg tgcggttcct aaacaagttg
    14281 caccagctcc tagtcgacca gctacgggca aaacagtcta tcgtgtcgat cgcaataaag
    14341 tgcaatctat cttacaagag gccgtcgaaa atcctgattt agcacgtcaa aatctaattc
    14401 gtttgcagaa tgcctgggga gaggtaattg aaagtctagg tgggccggac aaggctctgc
    14461 tagttggttc tcaaccggtt gctgccaatg aacaccatgc tattcttgct tttgagtcta
    14521 acttcaatgc tggtcaaact atgaaacgag acaatctcaa taccatgttt ggcaatatcc
    14581 tcagtcaggc ggcaggtttt tcacctgaga ttttagctat ttccatggag gaatggaaag
    14641 aagttcgcgc agccttttca gccaaagcca aatcttctca aactgaaaaa gaagtagaag
    14701 aaagcctgat tccagaagga tttgaatttt tggctgataa agtgaaggta gaggaagact
    14761 aaagaaagat ttcgtgatac aataagttta tgaataaaca acaatttatt attatggcgc
    14821 tgtttacagc tgctgagacc tattttttca atgaagcctg gatgactggc cgctatatta
    14881 tggcagcctt ttgggcaatt ttactcttta gaaatttccg agtcagttat gtgatgggca
    14941 aaatcgttga tgtcatcgat cagcatttta ataggaaaga ctagccctca gcttccagac
    15001 aaaatcaaag ccttttaggc ttttttttgt tatactagaa aagtatattt atagaatttt
    15061 tgctctattt ctggggaaat cagacgtttt tctagtaagt actgtaaaag ttttgaaaaa
    15121 gaaaggaact atcatgtcag tattagagat caaagatctt cacgttgaga ttgaaggaaa
    15181 agaaatttta aaaggggtta acctgaccct gaaaacagga gaaattgccg ctatcatggg
    15241 accaaatggt acaggtaaat cgactctttc tgccgctatc atgggaaatc caaactatga
    15301 agtaactaaa ggtgaagttt tgtttgatgg cgtaaacatc cttgagttgg aagtggatga
    15361 gcgtgcgcgt atgggacttt tccttgctat gcaataccca tcagaaatcc ctggaattac
    15421 caatgctgag tttcttcgtg ccgctatgaa tgcgggtaaa gaagatgatg agaagatttc
    15481 agttcgtgag tttattacta ggctagatga aaaaatggaa ttgctcaaca tgaaagaaga
    15541 aatggcagag cgttacctca acgaaggctt ctctggtggt gagaaaaaac gcaatgaaat
    15601 tcttcaactt ttgatgttgg agccaacatt tgctcttttg gacgagattg actcaggtct
    15661 tgatattgac gctcttaaag ttgtgtctaa aggtgtcaat gccatgcgtg gtgaaggttt
    15721 tggtgctatg atcatcactc actaccaacg tcttttgaac tatatcacac ctgatgtggt
    15781 acacgtgatg atggaaggtc gtgttgtcct ttctggtggt ccagaattgg ctgcgcgttt
    15841 ggaacgtgaa ggatacgcaa aattagctga agaacttggc tacgactaca aggaagaatt
    15901 gtaatttcct cgtatctttt aggagaagta aatgactaga gaaaatatta aacttttttc
    15961 agaaatgcac gctgaaccaa gctggttggc tgatctccgt caaaaagctt ttgacaagat
    16021 tgagactttg gaattaccag ttattgagcg tgtcaaattc caccgttgga atctgggtga
    16081 tggaacgatt acagaaaatg agccatcagc aaatgttcca gatttcacag ctttagatca
    16141 tcacttgaag ttggtgcaag taggaactca aactgttttc gaacaaactc cagttgagtt
    16201 agctgaacag ggtgttgtct tcacagactt tcactcagct ttagaagaaa ttccagagct
    16261 gatcgaagaa ttcttcatgt catctgttaa gtatgatgat gacaagttgg cggcttacca
    16321 cacagcttac tttaacagtg gtgctgtact ctatattcca gataacgtag aaatcacaga
    16381 gccaattgaa ggaattttct accaagatag cgatagcaat gtgccgttta acaagcatat
    16441 tatgattatc gctggtaaaa attctaagat taattatctg gagcgtttag agtcacgcgg
    16501 tgaaggaagt gccaaagcaa ctgctaatat cacagtagaa gtgattgcac gttcaggtgc
    16561 gcaagtcaag tttgctgcta tcgaccgtct aggtgaaaac gtcactgcct acattagccg
    16621 tcgtggtaaa ttaggcaacg atgcaagtat tgactgggct atcggtgtca tgaacgaagg
    16681 aaatgtcgtt gctgattttg atagtgactt gattggtaat ggtagctatg ctgacctcaa
    16741 ggttgtagct ctttcaagtg gtcgtcaggt acaagggatt gatactcgtg taactaacta
    16801 tggctgcaac tctatcggaa atatcctaca acatggggtt atccttgaaa aagcaacttt
    16861 gactttcaat ggtatcggcc acatcatcaa gggtgctaag ggagcagatg cccaacaaga
    16921 gagccgtgtt ctcatgcttt cagaccaagc gcgttcagat gctaacccaa ttcttttgat
    16981 tgatgaaaat gatgtcactg caggacacgc agcgtctatc ggacaagttg atccagaaga
    17041 catgtattac ctcatgagtc gtggattgga taaggcaact gcagagcgtt tggttgttcg
    17101 tggtttcctt ggatctgtta tcgtggagat tccagtcaag gaagttcgtg atgaaatgat
    17161 tgcaactatc gaagagaaat tgtcaaaacg ctaaggggaa gcctatgtta gatgtagaag
    17221 cgattcgcaa ggattttcca attttagatc agattgtcaa tgatgaacct ctggtctatc
    17281 tggacaatgc tgcgacgaca caaaaaccac tagtagttct gaaagctatt aacagttact
    17341 atgagcagga caatgccaat gttcaccgtg gtgtccatac cttagcggaa cgagcgacag
    17401 cttcttatga agctgctcgt gaaaccattc gtaagtttat taatgcaggc tctacaaagg
    17461 aagttctctt taccagagga acgacaacca gccttaactg ggtggcacgc tttgctgagg
    17521 aaattctcac tgagggagac caggtcttga tttcagtaat ggaacaccat tctaatatca
    17581 ttccatggca ggaagcttgt cgaaagactg gagcagagct tgtctatgtc tatcttaaag
    17641 acggtgcctt ggatatggag gatttgcgag ctaaattgac tgataaggtt aaatttgttt
    17701 ccctagctca tgcctccaat gttcttggtg tggtcaatcc gatcaaggaa atcactcaat
    17761 tagcccacca agttggggca atcatggtag tggatggtgc tcaatctaca cctcatatga
    17821 agattgatgt ccaggacttg gatctggact ttttcgcctt ttcgggtcac aagatggctg
    17881 gtccgactgg tatcggtgtc ctttacggca aagaaaagta tcttgagcaa atgtctccag
    17941 tagaatttgg cggcgagatg attgattttg tctacgagca atccgctagt tggaaggaat
    18001 tgccttggaa atttgaggct ggaacgccaa atatggcagg agctattgga cttgcgactg
    18061 cagttgatta tctggaaaag attggtatgg atgccgttga agctcatgaa caggaattga
    18121 ttgcgtacgt ctatccaaaa ctgcaggcaa ttgagggatt gaccatttac ggttctcagg
    18181 atttggctca acgttcgggt gttattgcct ttaacctagg tgatctccat cctcacgatc
    18241 ttgcgacggc tctggattat gaaggagtgg ctgttcgtgc tggtcaccat tgtgcgcaac
    18301 ccttgcttca gtatttggaa gtcccagcat cagctcgtgc aagtttttat atctataata
    18361 ccaaggcaga ttgcgacaaa ctagtcgatg ccctacaaaa gacaaaggag tttttcaatg
    18421 gcactttcta aactagatag cctttatatg gcagtggtag cagaccattc gaaaaatcca
    18481 catcaccaag ggaagttaga agacgctgag caaatcagtc tcaacaatcc gacttgtggg
    18541 gatgtcatca acctctctgt caagtttgat gcagaggacc gtttggaaga tattgctttt
    18601 ctaaattcag gatgcacgat ttcaactgct tctgctagta tgatgacaga tgccgtttta
    18661 ggaaaaacca aacaagaaat tttagaactg gcgactattt tttctgaaat ggttcaaggg
    18721 caaaaagatg agcgtcaaga ccaacttgga gacgcggcat tcttgtcagg tgttgccaaa
    18781 ttccctcaaa gaatcaagtg tgcaacccta gcttggaatg cccttaagaa aacaattgaa
    18841 aatcaagaaa aacagtaaga caagtttctt ttgtcttatg aattattaga aatgaagaaa
    18901 ggatattatg gctgaagaaa gagtagaacc aaaaccaatt gaccttggtg aatataaatt
    18961 tggtttccat gacgatgtag agcctgtctt atcgacagga aaaggactca acgaaggtgt
    19021 tattcgtgaa ttatctgctg ctaagggtga gcctgagtgg atgttggagt tccgtttgaa
    19081 gtcttatgaa accttcaaaa aaatgcccat gcaaacttgg ggagcagact tgtcagagat
    19141 tgactttgat gacttaatct actaccaaaa accatctgac aaaccagccc gttcttggga
    19201 tgatgtacct gaaaagatta aagaaacctt tgaacgtatc gggattccag aagctgaacg
    19261 tgcttattta gcaggggctt ctgcccagta cgagtcagaa gtggtttacc acaacatgaa
    19321 ggaagagttc caaaaattag gtattatctt tacagataca gattccgcac tcaaggaata
    19381 cccagactta tttaaacaat actttgcgaa gttggtaccg ccgacagata acaagttggc
    19441 agccctcaac tcagcagtat ggtcgggtgg aacttttatc tacgtgccaa aaggtgtcaa
    19501 ggtagatatt ccacttcaaa cttatttccg tatcaataac gaaaatatag gtcagttcga
    19561 acgtaccttg attatcgttg atgagggagc aagcgtccac tacgtagaag gatgtacagc
    19621 accaacatat tcaagcaata gcttgcacgc tgccattgta gaaatttttg ctttggacgg
    19681 agcttatatg cgttatacaa ctatccaaaa ctggtctgat aacgtctata acttggtaac
    19741 aaagcgtgct aaggctcaaa aggatgccac tgttgagtgg attgatggaa acttgggtgc
    19801 caaaacgact atgaaatatc catctgttta tcttgatgga gaaggagcgc gtggtaccat
    19861 gctctctatc gcctttgcta atgcagggca acaccaagac acgggtgcta agatgattca
    19921 caatgctcca cataccagct cgtctattgt gtctaaatcc atcgctaaag gtggaggaaa
    19981 ggttgactac cgtggacaag tcacctttaa caagaactct aagaaatctg tttcccacat
    20041 tgaatgtgat accattatca tggatgactt gtcagcatca gatactattc catttaatga
    20101 aattcacaac tcgcaagtgg ctttggaaca cgaagccaaa gtatctaaga tttcagaaga
    20161 gcaattgtat tatctcatga gccgtggatt gtcagaatct gaggcaactg aaatgattgt
    20221 catgggattt gtagaaccct ttacaaaaga acttccaatg gaatacgcag ttgagctgaa
    20281 ccgcttgatt agctatgaaa tggagggatc agttggataa aatttgattt tatactcttc
    20341 gaaaatctct tcaaaccacg tcagcatcgc cttaccgtat gtatggttac tgacttcgtc
    20401 agtttcatct acaacctcaa aacagtgttt tgagcaacct gcggctagct tcctagttta
    20461 ttctttgatt ttgagtatta gatttactca aaatcaagga ttttgaagat gaacttgtat
    20521 caaaaaatcg cggtttaaaa tcgcgatttt ttataatttc tcgttaacaa agcggacaaa
    20581 ctgattccac caaactttta agaagaaggc tttttcaatt ttcttgtctg ctaccatttc
    20641 gaaactaggg cgctctgtgg tgatgtaacc ttgaccaatc aagtccttgt cttcataagt
    20701 caaatggcca accactgttc cagcttcaag tggtgctggg attgctttgg aatcaggtgt
    20761 gaattgaaca gattgggaag attgattccc aacacgttcg attagataga tatcctctgg
    20821 agccactgca gttactgtat cttcttttcc atcttgtaca ggggctttgc tatcttgata
    20881 ggcatcgcct tgttgaacga ttttgcgaag tgtaaatgta gaagaaatat aatccattag
    20941 ggaagatgta gctgtaaatc gagcgtaagg attattgtct tgatgatctg catttaaaac
    21001 aactgtgatg actctcatgc ctttttcgac agtagtacca acaaaagact ctccagcctt
    21061 atctgttgtt cctgttttta gcccatcaaa accaccacgg taagcaggca taccttctaa
    21121 catgtagttg gttgaagtga ttgtcatccc agcaaaagta gaagaaggtt ttttggtgat
    21181 ttctaagact tgtgggtatt ttttgatgag gttgcgagca acgatagcga catcataagc
    21241 actaagctta ttttcctcat cttttttaga acctgggtaa atgttatccc ctagagtttc
    21301 attgttaaga cctgtcgtat tgacaacagt ggcatcctga attccccatt ccaagagttt
    21361 tgcccgcatc atatcgatga aatctttttc tgagccagca attttctcag ctagggcaat
    21421 agcggcgctg ttggcactag ataccagagt tgcttcaagc aactcttcga cagtataatt
    21481 acgggcctcc ataggaatat tactggcttc agaatttgtc gtcaattgat aaggataatc
    21541 agaaatatct acaggagtgg agagggtaat acttccgttt tccaaagctt catagaccag
    21601 ataagcagta atcaattttg ttatggaagc aatttcgaca ggttgcgttg catccttctc
    21661 atagagaatt ttaccagtat ttgcctcaac agcaatcgca tgtttagcgg caatggtaaa
    21721 atcttgagca acagcagtag aagcaccccc taaaagagag acagttaaca aagttaaaaa
    21781 tatttttttc atagtagtct tattctatca taaagaaaaa aaatattctt gctttaataa
    21841 ttcatctgtt aagctttttg aaaatatggt aaaataaagt gagggaggta actcatgttt
    21901 cgtagaaata aattattttt ttggaccaca gaaattttac tcttaaccat catcttttac
    21961 ctatggagac agatggggtc tttgattaac ccttttgtta gcgtgcttaa tacaattatg
    22021 attccatttt tattaggggg ctttctttat tatttgacaa accctattgt tactttctta
    22081 aataaagtct gtaaactcaa tcgtttgctt ggtattttaa ttaccttgtg tactttggtc
    22141 tggggaatgg tcataggtgt tgtctatctc ttacctattt tgattaatca gttatctagt
    22201 ttgattatat ctagtcaaac tatttatagt cgagtacaag acttaatcat agacttatct
    22261 aattatcctg cgctccagaa tttggatgta gaagctacaa ttcagcagtt aaacttatcc
    22321 tatgttgata ttcttcaaaa tatcctaaat agcgtatcaa atagtgtggg gagcgtcttg
    22381 tcagctctta tcagtactgt tttgattttg attatgactc cagttttttt ggtttatttc
    22441 ttattagatg gacataaatt cttgcccatg cttgaaagaa cgattctaaa gagggatcgc
    22501 ttgcatattg caggcttatt aaagaattta aatgcgacga ttgctcgcta tattagtgga
    22561 gtttcgattg acgcaatcat tataggttgt ttggcttata ttggctatag tattattggt
    22621 ttaaaatatg ctttagtttt tgccattttt tctggtgtag ccaatttaat tccttatgtg
    22681 gggccaagta ttggtttgat tcctatgatc atcgcaaata tattcactga tccccataga
    22741 ctgctgattg cagtgattta tatgcttgtt gttcagcagg tagatggcaa tatcttatat
    22801 cctcgaatcg taggaagtgt tatgaaggtt catccaatca cgattttagt tttacttttg
    22861 ttgtcaagca atatctatgg tgtagttgga atgattgtcg cagtgccaac ctattctatc
    22921 ttgaaagaaa tttctaagtt cttatcccgt ttgtatgaaa atcataaaat aatgaaagaa
    22981 cgagaaagag aattagctaa gtaaaagtca ggagaaccct gatttttctt tactggaagt
    23041 ggcctttaga ttagaagact gaaaataagt taaagtctta aactaatttt cacagctaag
    23101 aatagtagaa gttaatctga taaaaatcga aaaaaccagt ggaattctgt gtcagggtaa
    23161 gttccactgg ttttcatagt ctattaaagt tcgaatgaaa cctatttata gtagattgaa
    23221 actagaatag tacacctcta attctaaaac attgttagaa atcgatttga ctgtcctgat
    23281 ctattcgttc tattcttatt tcattttact atattttggt gcaataagtg aaaagtagtc
    23341 cgaataatat aaggattgat tttatagttt ttaaactcaa atgaattgaa ataaagagag
    23401 tacgaaaatt ctcatctgaa agtattttag aataattctc ttcgtgaatt tcttcaaaac
    23461 agatagcttc atcttaggta tgtgatttct ttttgcattt ttgagttaga taaggtataa
    23521 tggttttatt gtcttttggg gtcgttacgg attcgacagg cattatgagg catattttgc
    23581 gactcgtgtg gcgacgtaaa cgctcagtta aatataactg caaaaaataa cacttcttac
    23641 gctctagctg cctaaaaacc agcaggcgtg acccgatttg gattgctcgt gttcaatgac
    23701 aggtcttatt attagcgaga tacgattaag ccttgtctag cggtttgata agagattgat
    23761 agactcgcag tttctagact tgagttatgt gtcgaggggc tgttaaaata atacataacc
    23821 tatggttgta gacaaatatg ttggcaggtg tttggacgtg ggttcgactc ccaccggctc
    23881 cattattcct ttgcattctt ttgcattcct tggtaaaacg ttgttaaatc aacgtttttt
    23941 atttttatct ttggtattcc tttgcattct tttgctaaaa agggagtcac aaacagaccc
    24001 tattttaaaa aaaggataga aaaaaggata caacatttgt cgcatcctaa aaataatctt
    24061 ttttccacgg aagacatggg attcgaaccc acgcacgcta ttacacgcct accgcgtttc
    24121 caacacggcc tcttaagcct cttgagtaat cttccaatac ttactcaaat agtctaccat
    24181 aaaggctctt atcttgcaat aaaaattcta gaaataagaa aaatgataga ttttgaaaga
    24241 aaatgataaa aaatgcttga cttcaaaaga aagtatgata gaatgaatag tgtaaacgat
    24301 aacaggaggt gattcggtgt taaaaacaga gcggaagcaa ctgattttag aggagttaaa
    24361 tcaacatcat gtagtttctc tagaaaaatt agttagtttg ctagaaacgt cagaatcaac
    24421 ggttcgaaga gacttggatg agttggaagc ggaaaacaag cttcgtcgtg tgcatggtgg
    24481 agcagaactc ccccactcct tacaggaaga agaaaccatt caagaaaaat ctgtcaaaaa
    24541 ccttcaagaa aagaagttgc tggctcagaa agcagcctct ctcattaaag aaaaagatgt
    24601 catctttatc gatgctggaa caacaactgc ttttttgatt catgaattgg tcaataagaa
    24661 tattacagtt gtgaccaact ccattcacca tgccgctcag ttggttgaaa agcagattcc
    24721 aactgtcatg gttggaggaa acgtcaagac ggcgacagat gctagtatcg gtggcgttgc
    24781 tcttaaccag attaaccaat tgcactttga ccgtgccttt atcggaatga atggtgttga
    24841 cgatggctat tatacgactc ctgatatgga ggagggagct gtgaaaagag ctattttgga
    24901 gaatgccaag cagacctacg tcttggtgga ttcgtcaaaa attggacaaa cttgctttgc
    24961 caaggtagca ccactcaaac gcgctatcgt tatcactagt caagggcatg agctcttgca
    25021 ggttattaag gagaaaacgg aggtaataga agtatgattt atacagtcac actcaatcca
    25081 tccattgact atatcgttcg tttggaccaa gtcaaagttg gtagtgtaaa tcgtatggac
    25141 agtgatgata agtttgctgg tgggaaagga atcaatgtca gccgtgtctt gaaacgtttg
    25201 aatataccaa atacagcgac gggatttatc ggtggcttta ctggtaaatt tatcacagat
    25261 actttagcag aggaagaaat cgagacacgt tttgtccagg tggcagaaga tactcgtatc
    25321 aatgttaaaa tcaaagcaga ccaagaaaca gaaatcaacg gaacgggtcc aactgttgaa
    25381 tcggttcagc tagaagaatt gaaagctatt ttatctagtc tgacagcaga agatacagtt
    25441 gtctttgcag gttcaagtgc taaaaatcta ggcaatgtta tctataagga tttgatttcc
    25501 ttgacgcgcc agactggtgc gcaagtggtc tgtgactttg aaggacagac cttaattgat
    25561 agtttggact accagcctct tcttgtaaaa ccaaacaatc atgaacttgg agcgattttt
    25621 ggggttaaac tcgaaagttt agatgaaatt gagaaatacg ctcgtgagtt actggctaag
    25681 ggtgctcaaa atgttattat ctctatggct ggtgatggtg cccttcttgt cacatctgag
    25741 ggagcttact tcgctaaacc aatcaaagga acagtcaaaa attcagttgg agctggtgat
    25801 tctatggttg ctggattcac aggtgaattt gtcaaatcaa aagacgtagt agaagccttc
    25861 aaatggggag tggcttgcgg aacggcaact accttctcag atgacttggc aacggcggaa
    25921 tttattaaag aaacatatgg aaaagttgag gtagaaaaac gatgaaaatt caagacctat
    25981 tgagaaaaga tgtcatgttg ctagatttgc aggcaactga aaaaacagct gtcatcgacg
    26041 agatgattaa aaatttgaca gaccacggtt atgtaacaga ttttgaaaca tttaaagaag
    26101 gaattttggc gcgtgaagct ttgacttcta ctggtttggg tgatggaatc gcaatgcctc
    26161 acagcaaaaa cgctgctgtc aaagaagcga cagttctatt tgctaagtca aataagggtg
    26221 ttgactacga gagcttggat ggacaagcaa ctgacctctt cttcatgatt gcagctccag
    26281 aaggtgccaa tgatactcac ttggcagcct tggcagaatt gtctcaatac ttgatgaaag
    26341 acggttttgc agacaaactt cgtcaagcaa catctgcaga ccaagttatc gaactttttg
    26401 accaagcttc agaaaaaact gaggaacttg ttcaagcacc tgctaatgac tctggtgact
    26461 ttatcgtagc tgttacagct tgtacaacag gtattgccca cacttacatg gcccaagaag
    26521 cccttcaaaa agtagctgct gaaatggggg ttggtatcaa ggtcgaaacc aacggtgcta
    26581 gcggtgttgg aaatcaacta actgcagaag atatccgtaa ggctaaagct attatcattg
    26641 cagcagacaa ggccgttgaa atggatcgat ttgatggaaa accattgatc aatcgtccag
    26701 ttgctgacgg tatccgtaag acagaagagc taattaactt ggctctttca ggagatactg
    26761 aagtctaccg tgccgctaat ggtgccaaag ctgcaacagc ctctaacgaa aaacaaagcc
    26821 ttggtggtgc cttgtacaaa cacttgatga gtggtgtatc tcaaatgtta ccattcgtta
    26881 tcggtggtgg tatcatgatt gcccttgcct tcttgattga cggtgctttg ggtgttccaa
    26941 atgaaaacct tggcaatctt ggttcttacc atgagttagc ttctatgttc atgaaaattg
    27001 gtggagctgc ctttggtttg atgcttccag tctttgcggg ttatgttgcc tactctattg
    27061 ctgaaaaacc gggtttggta gcaggtttcg tggctggtgc tattgccaaa gaaggttttg
    27121 cctttggtaa aattccttat gccgcaggtg gtgaagcaac ttcaactctt gcaggtgtct
    27181 catctggttt cctaggtgcc cttgttggtg gatttatcgc aggtgccttg gttcttgcca
    27241 tcaagaaata cgttaaagtt cctcgttcac tcgaaggtgc taaatcaatc cttctattgc
    27301 cacttcttgg aacaatcttg acaggatttg ttatgctagc tgtgaatatc ccaatggctg
    27361 caatcaacac tgctatgaat gacttcctag gcggtcttgg aggaggttca gctgtccttc
    27421 ttggtatcgt ccttggtgga atgatggctg ttgacatggg tggaccagtt aataaagcag
    27481 cttatgtctt tggtacaggt acgcttgcag caactgtttc ttcaggtggt tctgtagcca
    27541 tggcagcagt tatggctgga ggaatggtgc caccacttgc aatctttgtc gcaactcttc
    27601 ttttcaaaga taaatttact aaggaagaac gtaactctgg tttgacaaac atcatcatgg
    27661 gcttgtcatt tatcactgag ggagcgattc catttggtgc cgctgaccca gctcgtgcga
    27721 ttccaagctt catccttggt tcagcagtag caggtggact cgttggtctt actggtatca
    27781 aactcatggc gccacacgga ggaatcttcg ttatcgccct tacttcaaat gctctccttt
    27841 acctcgtttc tgtcttggta ggagcaatcg taagtggtgt ggtttatggt tacctacgca
    27901 aaccacaagc ataaaaaata gaaaaatgaa aagattggac cgtttggtgc agtctttttc
    27961 tcttcccgaa atgcctgtga aatatggtat aatagaagaa tggcaaacaa gaatacaagt
    28021 acaacaagac ggagaccgtc taaagcagaa ctggaaagaa aagaagcgat tcaacgaatg
    28081 ttgatttcgt taggaattgc gattttattg attttcgcag ccttcaaatt aggggctgca
    28141 ggtataaccc tttataattt aattcgcttg ctagtgggta gcctagctta tctggcgata
    28201 ttcggcctat taatctatct cttctttttc aagtggatac gaaaacagga aggactctta
    28261 tctggctttt tcaccatatt tgctggctta ctcttgattt ttgaggccta cttggtttgg
    28321 aaatatggtt tggacaagtc cgttctaaaa gggaccatgg ctcaggttgt gacagatctg
    28381 actggttttc gaacgactag ctttgctgga gggggcttga tcggggtcgc tctttatatt
    28441 ccaacagcct ttctcttttc aaatatcgga acttacttta ttggttctat cttgatttta
    28501 gtgggttctc tcctagtcag cccttggtct gtttacgata ttgctgaatt tttcagtaga
    28561 ggctttgcca aatggtggga agggcacgag cgtcgaaaag aggaacgctt tgtcaaacaa
    28621 gaagaaaaag ctcgccaaaa ggctgagaaa gaggctagat tagaacaaga agagactgaa
    28681 aaagccttac tcgatttgcc tcctgttgat atggaaacgg gtgaaattct gacagaggaa
    28741 gctgttcaaa atcttccacc tattccagaa gaaaagtggg tggaaccaga aatcatcctg
    28801 cctcaagctg aacttaaatt ccctgaacag gaagatgact cagatgacga agatgttcag
    28861 gtcgattttt cagccaaaga agcccttgaa tacaaacttc caagcttaca actctttgca
    28921 ccagataaac caaaagatca gtctaaagag aagaaaattg tcagagaaaa tatcaaaatc
    28981 ttagaagcaa cctttgctag ctttggtatt aaggtaacag ttgaacgggc cgaaattggg
    29041 ccatcagtga ccaagtatga agtcaagccg gctgttggtg taagggtcaa ccgcatttcc
    29101 aatctatcag atgacctcgc tctagccttg gctgccaaag atgtccggat tgaagcacca
    29161 atccctggga aatccctaat cggaattgaa gtgcccaact ccgatattgc cactgtatct
    29221 ttccgagaac tatgggaaca atcgcaaacg aaagcagaaa atttcttgga aattccttta
    29281 gggaaggctg ttaatggaac cgcaagagct tttgaccttt ctaaaatgcc ccacttgcta
    29341 gttgcaggtt caacgggttc agggaagtca gtagcagtta acggcattat tgctagcatt
    29401 ctcatgaagg cgagaccaga tcaagttaaa tttatgatgg tcgatcccaa gatggttgag
    29461 ttatctgttt acaatgatat tccccacctc ttgattccag tcgtgaccaa tccacgcaaa
    29521 gccagcaagg ctctgcaaaa ggttgtggat gaaatggaaa accgttatga actctttgcc
    29581 aaggtgggag ttcggaatat tgcaggtttt aatgccaagg tagaagagtt caattcccag
    29641 tctgagtaca agcaaattcc gctaccattc attgtcgtga ttgtggatga gttggctgac
    29701 ctcatgatgg tggccagcaa ggaagtggaa gatgctatca tccgtcttgg gcagaaggcg
    29761 cgtgctgcag gtatccacat gattcttgca actcagcgtc catctgttga tgtcatctct
    29821 ggtttgatta aggccaatgt tccatctcgt gtagcatttg cggtttcatc aggaacagac
    29881 tcccgtacga ttttggatga aaatggagca gaaaaacttc ttggtcgagg agacatgctc
    29941 tttaaaccga ttgatgaaaa tcatccagtt cgtctccaag gctcctttat ctcggatgac
    30001 gatgttgagc gcattgtgaa cttcatcaag actcaggcag atgcagacta cgatgagagt
    30061 tttgatccag gtgaggtttc tgaaaatgaa ggagaatttt cggatggaga tgctggtggt
    30121 gatccgcttt ttgaagaagc taagtctttg gttatcgaaa cacagaaagc cagtgcgtct
    30181 atgattcagc gtcgtttatc agttggattt aaccgtgcga cccgtctcat ggaagaactg
    30241 gagatagcag gtgtcatcgg tccagctgaa ggtaccaaac ctcgaaaagt gttacaacaa
    30301 taaaaaaata gcttctttcc aagtttggag ggaagctatt ttagtggcta ttgattgctt
    30361 ttattttctg aagttggcgc attggactgt ttttcgtttt cagtagcagg tttacttgaa
    30421 gcaggagtag aagagtcctg agttgctgtt ttctgatctt cttttttctc ttccttgacg
    30481 ctagattttg gtgtttcctc ttgctgtgtt ttttcttgac tagtgttagt ctctttagtt
    30541 ggactggtgt tttccttagg ggattccttt tggatttctt tgacaatggt tgtcgtctgg
    30601 cttgtcgtag gttctttttt aatatttttg ttattatcca aggcgttcat aatcgtgata
    30661 cttgcggtaa tcaagccaag gatactagcg attgtagcaa cggttttttg aaagactgta
    30721 aagccttttt tgatgtgttc tgtttttggt tttgaagttc tacgatagtt agacatacat
    30781 tctctccttt gattatgatt tattatactc tgtttcttta aaaaaatctt aaaaaaagag
    30841 aaatttccct ttcttgtcgc aagctccgtt tcatgataca atagaggaag tgattttaga
    30901 aagcgtgaga aacatgattt actttgataa ttcagcgacg accaagcctt atcctgaagc
    30961 ccttgaaacc tatatgcagg tcgcttcaaa aattttagga aacccatcta gtctccatcg
    31021 tttgggagac caagcaacac gaatcttaga tgcttctcgc caacagattg cagatttaat
    31081 cggtaagaaa agcgatgaaa tcttctttac ctcgggtgga acagaagggg ataactggct
    31141 tatcaagggt gtggcctttg aaaaagctca gtttggcaag cacatcattg tttcagccat
    31201 tgaacatcca gcagtcaaag agtcagccct ctggttgaaa agtcaaggat ttgaagtgga
    31261 ttttgctcca gttgataaga aaggcttggt cgatgttgag gcgttagcag gtttgatacg
    31321 gcatgataca atcctcgttt ccatcatggc tgtgaacaat gaaatcggct ctatccaacc
    31381 tattgaggct atttcagaat tcttggcaga caagccgact atttccttcc acgttgatgc
    31441 ggttcaggcg cttgccaaaa ttccgactga aaagtatctg acagaacggg tggattgcgc
    31501 gactttctct agtcacaagt tccacggggt tcgaggtgtt ggctttgtct atatcaaatc
    31561 tggcaagaag attacacctc ttcttacagg tggtggccag gagcgagatt atcgttcgac
    31621 aactgaaaat gtggcaggga ttgcagcgac agccaaggcc ctccgtttgt ctatggaaaa
    31681 gctagatatc tttaggagca agactgggca gatgaaggca gtgattcgcc aagctcttct
    31741 gaactatccg gatatttttg tcttttcaga tgaggaaaac tttgcacctc atattctgac
    31801 ttttggaatc aaaggtgttc gaggtgaagt catcgttcac gcctttgaag actatgatat
    31861 tttcatctca acaacctcag cttgttcatc taaggcagga aaaccagccg gtaccttgat
    31921 tgccatggga gtggacaaag ataaggccaa gtcagctgtg cgtcttagcc tagacttgga
    31981 aaatgatatg agtcaggtcg agcagttttt gaccaagtta aaattgattt acaatcaaac
    32041 tagaaaagta agataggagc attcatgcag tattcagaaa ttatgattcg ctacggagag
    32101 ttgtcaacca agggtaaaaa ccgtatgcgt ttcatcaata aacttcgtaa taatatttcg
    32161 gacgttttgt ctatctatac ccaagttaag gtaacagcag atcgcgaccg tgcccacgct
    32221 tacctcaatg gagctgatta cacagcagtt gcagaatctc tcaaacaagt ttttggaatt
    32281 caaaactttt ctcctgttta taaggttgaa aaatctgtag aagttttgaa gtcttctgtc
    32341 caagagatta tgcgggacat ctacaaggaa ggtatgacct ttaagatttc tagcaagcgt
    32401 agcgaccaca actttgaact tgatagtcgt gaactcaacc aaacacttgg aggggctgta
    32461 ttcgaagcca ttccaaatgt gcaagttcaa atgaaaagtc ctgacatcaa tcttcaggtg
    32521 gagattcgtg aagaagcagc ctatctttct tatgaaacca ttcgtggggc tggtggtttg
    32581 ccagttggaa cttcaggtaa agggatgctc atgttgtcag gagggattga ctcacctgta
    32641 gcaggttatc ttgctcttaa gcgtggggtg gatatcgagg cagttcactt tgctagtcca
    32701 ccatatacta gtcctggtgc cctcaagaaa gcgcaggact tgacccgtaa attgaccaag
    32761 tttggcggaa atatccagtt tatagaggtg cctttcacag agattcaaga ggaaatcaaa
    32821 gccaaagcgc cagaagctta tttgatgact ctaactcgtc gctttatgat gcggattact
    32881 gaccgtattc gtgaggtacg aaatggtttg gttatcatca atggggaaag tctaggtcaa
    32941 gtagccagcc aaacccttga aagtatgaag gctatcaatg ctgttaccaa cactcccatc
    33001 attcgtcctg tggttaccat ggacaagttg gaaatcattg acatcgccca ggaaatcgat
    33061 acctttgaca tttcaatcca accgtttgaa gactgttgta ccatttttgc accagatcgt
    33121 ccaaaaacaa atcctaaaat taagaatgcg gagcagtacg aagcgcgtat ggatgttgaa
    33181 ggcttggttg agcgagcagt ggctggaatc atgattactg aaatcacacc tcaagccgaa
    33241 aaagatgaag ttgatgactt gattgacaat ctgctctaat tcagaaaatc caaaagaata
    33301 gcgaaaatca gtaaaaaaag ttagtttttt ctctaaaaac aggtaaaaaa ctaacttttt
    33361 ttatttttat gatataatga tataaaattt tgaatataga gagttttctg acaatgaatc
    33421 aatcctactt ttatctaaaa atgaaagaac acaaactcaa ggttccttat acaggtaagg
    33481 agcgccgtgt acgtattctt cttcctaaag attatgagaa agatacagac cgttcctatc
    33541 ctgttgtata ctttcatgac gggcaaaatg tttttaatag caaagagtct ttcattggac
    33601 attcatggaa gattatccca gctatcaaac gaaatccgga tatcagtcgc atgattgtcg
    33661 ttgctattga caatgatggt atggggcgga tgaatgagta tgcggcttgg aagttccaag
    33721 aatctcctat cccagggcag cagtttggtg gtaagggtgt ggagtatgct gagtttgtca
    33781 tggaggtggt caagcctttt atcgatgaga cctatcgtac aaaagcagac tgccagcata
    33841 cggctatgat tggttcctca ctaggaggca atattaccca gtttatcggt ttggaatacc
    33901 aagaccaaat tggttgcttg ggcgtttttt catctgcaaa ctggctccac caagaagcct
    33961 ttaaccgcta tttcgagtgc cagaaactat cgcctgacca gcgcatcttc atctatgtag
    34021 gaacagaaga agcagatgat acagacaaga ccttgatgga tggcaatatc aaacaagcct
    34081 atatcgactc gtcgctttgc tattaccatg atttgatagc agggggagta catctggata
    34141 atcttgtgct aaaagttcag tctggtgcca tccatagtga aatcccttgg tcagaaaatc
    34201 taccagattg tctgagattt tttgcagaaa aatggtaagt taagaaagga aaaaacgaaa
    34261 tgcatattga acatcttagc cactggagtg gtcatcttaa ccgtgaaatg taccttaacc
    34321 gttatggaca tggtgggatt ccagttgtgg tctttgcttc atcaggtggt agtcacaacg
    34381 aatactatga ttttggcatg attgatgcct gtgcttcctt tatcgaggaa ggccttgtcc
    34441 agttctttac cctatctagt ttggatagtg agagctggtt ggctacttgg aaaaatgctc
    34501 atgaccaagc ggaaatgcac cgtgcctacg aacgttatgt gattgaggag gccattcttt
    34561 tatcaagcac aagacaggtt ggtttgatgg catgatgacg acaggttgct ctatgggagc
    34621 ctatcatgca ctcaatttct tcctccagca tccagatgtc tttaccaaag tgattgctct
    34681 cagtggtgtt tacgacgcac gtttctttgt cggtgattac tacaacgatg atgctattta
    34741 ccaaaactcg ccagtagatt atatttggaa ccaaaacgac ggctggttta ttgaccgtta
    34801 ccgtcaggca gagattgtgc tgtgtacggg gcttggagcc tgggaacaag atggtttgcc
    34861 atccttttac aagctcaaag aagcctttga caagaaacaa attccagcct ggtttgctga
    34921 atggggacat gatgtcgccc atgactggga atggtggcgt aaacaaatgc cttatttcct
    34981 cggtaatctc tatttataaa aggagttacc tatgaattac cttgttattt ctccctacta
    35041 tccacaaaac tttcaacagt ttaccatcga actagctaat aaaggcatca cagtcttggg
    35101 aattggtcaa gagtcttacg agcaattgga tgagcccttg cgcaatagct tgaccgagta
    35161 ttttcgtgtt gataatcttg agaacataga tgaagtcaaa cgtgcagttg cttttctctt
    35221 ttataaacat ggtccaattg gccgcatcga gtctcacaat gaatactggc ttgagctaga
    35281 cgcaacactc agagaacaat tcaatgtttt tggtgccaaa ccagaggatc tcaaaaagac
    35341 gaaatataag tctgaaatga agaaactttt caaaaaagca ggtgttcctg tggtacctgg
    35401 agctgttatc aagacggaag cagatgttga tcaagcagtg aaagaaatcg gtcttccaat
    35461 gattgccaaa cctgataatg gagtgggagc agccgcaacc tttaaacttg agacagaaga
    35521 cgatatcaat cacttcaagc aagaatggga ccattcaacc ctttatttct ttgaaaaatt
    35581 tgtcacttcc agcgaaatct gtacctttga cgggctcgtg gacaaggatg gaaagattgt
    35641 cttctcaaca acctttgact acgcctatac accgcttgac ctcatgattt ataagatgga
    35701 caattcttat tatgtgctca aggatatgga tcctaaactg cgcaagtatg gggaagcaat
    35761 tgtcaaagaa tttggtatga aagaacggtt tttccatatt gagttcttcc gtgaggggga
    35821 cgattatatt accatcgagt acaataaccg ccctgcaggt ggttttacca ttgatgttta
    35881 taactttgct cattccttgg acctttatcg tggctatgca gctattgtcg caggagagga
    35941 gttcccggcg tcagactttg aaactcagta ttgtttggct acttctcgcc gtgcaaatgc
    36001 tcactatgtt tattcagaag aggatttgct tgccaaatat agccagcagt tcaaggttaa
    36061 aaaagtcatg ccagctgcct tcgcggaact tcaaggagat tacctgtata tgctgaccac
    36121 tccgagtcga caagaaatgg agcagatgat tgcagatttc ggacaacgtc aagaataaga
    36181 actatcggat taaggaaatt aactccctta atccttttgt tttgtctgat aaaaaataag
    36241 agcatcccaa caaggtagct atcataaaac ttgttcgata actatttgaa gcaggattag
    36301 gtggtcagaa attaaatttt aatatttcaa ttgagtcata gtattgtgtt tgcgtatcct
    36361 taaatcagct aaaaggatcc atgacgacac ctatacgata tagttttcaa gataccaaac
    36421 aagtctatta atattcaatg aaaatcaaag agcaaactag gaagctagcc gcaggtttct
    36481 caaaacactg ttttgaggtt gtggatagaa ctgacagagt cagtatcata tacctacggc
    36541 aaggtgaagc tgacgtggtt tgaagagatt ttcgaagagt ataaaatatt caggtgacgc
    36601 atagatatag ttaattgaag ctttgtttga aatctgataa aataatgata ttactaagtt
    36661 ttaaaaacta aagaaaaggg aagatatgat tacaggcgaa ttaaaaaata aaatcgatca
    36721 gctgtgggaa attctttgga cagaaggaaa cgcaaatcct ttaacaaata ttgaacagtt
    36781 gacttatctc ttatttatga aagatttgga tagtgtcgag cttggacgtg aaagtgatgc
    36841 tgaatttcta gggattcctt atgagggagt ttttccaaaa gataaacctg aataccgttg
    36901 gtcaactttt aaaaatatag gagatgctca ggaagtttat cgtttaatga ctcaggagat
    36961 ttttccgttt attaaaaatc tcaaggggga tacagatgat acagcctttt cacgatatat
    37021 gcgagaagct atttttcaaa taaataaacc tgctacgctt caaaaggcaa tttctatctt
    37081 agatgttttt ccaactaggg gattagatgt agattttgat aatgacaaac aaagtattac
    37141 tgatatcgga gatatctatg aatatctgtt atcaaaattg tcgaccgcag gtaaaaatgg
    37201 acagttccgt acacctcgtc acatcatcga tatgatggtt gagttgatgc aaccgactat
    37261 caaagatatc atctcagatc ccgctatggg ttctgctggc ttcttagtat ctgctagccg
    37321 ttacttaaag cgtaagaaag atgaatggga aaccaataca gataatatca atcattttca
    37381 taatcagatg tttcatggaa atgatacgga tacgactatg ttgagacttg gggcgatgaa
    37441 catgatgcta catggagtag aaaatccaca aatcagttac cttgactcgc tgtctcaaga
    37501 taatgaagaa gccgataaat atactttggt tttagcaaat cctcctttta agggctcact
    37561 tgactacaat tcaacctcta atgaccttct tgcaaccgta aaaaccaaaa aaacagaatt
    37621 actctttctt tctcttttct tgcgaacttt aaaaccaggt ggacgagcag cagttatcgt
    37681 acctgatggt gtcctttttg gttcgtctaa agctcataaa ggaattcgtc aggaaattgt
    37741 agagaatcat aagcttgatg ctgtaatctc aatgcctagt ggtgtgttca agccttatgc
    37801 tggagtttca actgccattc tcatctttac aaaaactggt aatggtggta ctgacaaagt
    37861 ctggttttac gatatgaaag cggatggttt aagtttggat gataagcgac aaccgattag
    37921 cgacaatgat attccagata ttatcgaacg ctttcatcat cttgaaaaag aagcagaacg
    37981 tcagagaacg gatcaatctt tctttgttcc agttgctgag ataaaggaaa atgattatga
    38041 tttgtctatc aataaatata aagagattga gtatgaaaaa gttgagtatg aaccaacaga
    38101 ggtcatctta aagaaaatca atgatttaga aaaagaaatt caagctggct tggctgaatt
    38161 agaaaaatta ctcaagtaga gaggtggctg tatgaaaaaa gtgaagttgg gggaagtctt
    38221 atctctaaaa aaaggcaaga aagccactgt acttgctgaa caaacaactc taagccaacg
    38281 ttatattcaa atagatgatt taagaaataa taataattta aaattcactg aaagtttaaa
    38341 tatgactgaa gcactcccag atgatattct gatagcatgg gatggagcta atgcaggaac
    38401 agttggttat ggattatcgg gagctgttgg tagtacaatt acggtcttaa aaaagaatga
    38461 gcgatacaaa gaaaaaatta tatcagatta cttgggagtc tttttggaaa gtaaatcgca
    38521 gtatttacga gaacattcaa caggtgcaac aattcctcat ttaaacaaga atatattact
    38581 tgatttacaa ttagaattgc taggtatcga agaacaagag aacattatct gtattcttaa
    38641 tacgattaaa aggcttatta ctaaaagaaa attgcagtta gatgaactaa acttgctcgt
    38701 caaatcccga tttaacgaga tgtttgggga tgttattcta aatgaaaaag aatggaaagt
    38761 tagcaaatgg aacgagattc taacaataag aaatggtaaa aatcaaaaac aggttgaaga
    38821 tgctgatggg aaatttccta tttatggaag tgggggaatt atgggatatg ctaaagattg
    38881 gatagtgaag aaaaattcag ttattattgg acgtaaagga aatataaata aacctatttt
    38941 agttagagaa aatttttgga atgtagatac agcatttgga ttagagcctg ttttagaaaa
    39001 aataaactca gaatatttat tctatttttg tcaattatac aattttgaga agttaaataa
    39061 ggctgtaaca atacctagtt taactaaatc agatttatta aatatttcca tccccctccc
    39121 cccactagcc ctccaaaatg agttcgcaga ctttgtagcc ctggtcgaca aatcacaatt
    39181 tgcttgtgag atagctataa aagtgtggag aaatagcttg aaatttagta taatatagct
    39241 aaactatttg tttaaagtga gaaaaaaatg ggaaatttta gctttctttt aaaaaatgac
    39301 gaatatgaat ctttttcaaa accttgcatt gaagctgaga atatgattgc tacatcaact
    39361 gtggctactg cctttatggc gcgtcgtgct ttagagcagg ctgtccattg gatatatagt
    39421 cacgattcat atttagaagc tccctatcgt gctactctat cttctttagt atgggatgat
    39481 gattttaggg atatcgtaga ttctgaactc cacaagcaga tagttctgtt gattcggtgg
    39541 ggaaaccatg ctgctcatgg tggtgaaatt aaggaacgag aagcgatttt agctttgcat
    39601 catttgtatc agtttgttaa ttttatcgat tattgttaca gcaatgagtt tgtggagcgt
    39661 tattttgatg agaagtgctt accactttca gcaaacatca aataccgaga aactccacaa
    39721 tctatgataa agttacaaga cagtttacca gaactgcctg attttcatga acagatggct
    39781 gctcagtccg tagaagttca agagacttat actgaaaaac gtgagactgc agcgcaacgg
    39841 caagatgtgc ctttccatat tgatcaatta tctgaggcag agacaagaaa gctctttatt
    39901 gatatcgatc tccgtttagc aggatggata tttgaagaaa actgtcgtgt tgagatagcc
    39961 gttgatggtc tcaagcacgg ttcaggaatt ggttactgtg actatgtact ttatggtaaa
    40021 aatgggaaaa ttttagcgat tgtggaggct aaaaaagcct ctgtcaatcc agaagtaggg
    40081 gaagtacagg tcaaagaata tgctgaagct ttggagaaac atatcggcta tcagccaatt
    40141 tgctttatta caaatgggtt gaagcactat atacttgatg gtccgaaccg ccgccagatt
    40201 gcaggctttt actctcaaga agaattgcaa ttagtgatgg atagacgtca tcttcaaaaa
    40261 ccgcttgagg atatttctag taaaattagg gacgatattt ccgggcgtca ctaccaaaaa
    40321 catgccattg caagcgtttg tgaagctttc tctgatcatc gtagacaggc acttttggtt
    40381 atggcaactg gggcggggaa aactcgtaca gcagtttctc tagttgatat cttatcacgt
    40441 cataactggg taaaaaacgt tctcttctta gccgatagaa cttccttggt taagcaagca
    40501 tatgattcgt ttagaaaatt actcccagat ctttccgttt gtaacttctt agaagataaa
    40561 gaaggagctc aatcaagtcg aatggtcttt tcaacttatc cgaccatgat tggagcgatt
    40621 agtggtcaag aagaagtaaa tcaacgccct tttactgttg ggcattttga ccttatcata
    40681 attgacgaat ctcaccgttc tatttatcag aaatacaagt ccatttttga ttattttgat
    40741 gcaaggattg taggcttaac agctactccg cgtcaagatt tagataaaaa cacctatgga
    40801 ttctttaatt tggagaatgg ggttccaaca tatgcatatg atttggaaga ggctgttaaa
    40861 gacggatatt tagtagccta tcattctatc gaaaccaaac tgaaactacc tacggatggt
    40921 ctacattatg atgatttgtc cgaagaagaa aaggaacatt ttgatagcaa atttgaagac
    40981 gatagctgtg aaaaagatat tgatgggagt gtgtttaatt cctttatttt caataaaagt
    41041 acagtagaaa ttgttttaaa tgaactcatg acaagaggaa ttcagacagc ctcgggtgat
    41101 gaaattggta aaactattat ttttgctaaa aatcatgacc atgcggaata tatcagaggt
    41161 atttttaaca accgctatcc tgaaaaaggg agcgactatg ctcaggtgat tgattatagt
    41221 attaagcatt atcagacctt gattgatgat tttaaaatta aggagaagta tcctcaaatt
    41281 gcgatttctg tcgatatgtt agatacaggt attgatgtac cagaggttgt taatttagtc
    41341 ttcttcaaga aagtacgctc taaaactaag ttttggcaga tgattggtcg aggaacccgt
    41401 ctatgtaaag atttatttgg acctgagcag gataaggaaa acttcttggt atttgattat
    41461 ggggacaatt ttgattattt tcgtgcagat ccaagagatg gagagggtcg tcacattgtt
    41521 tcgctgactc agcgtttatt taatatcaaa gtggacttga ttcgagaact tcagggactc
    41581 caataccaag aagatcagtt tgcgagagca taccgtcagc agcttgtctc ggaacttcaa
    41641 ggtcgtatag agagcttaaa tgagttggac ttcagggttc gtatggtttt agatacagtt
    41701 tatagctata ggaaattgga aagttggcag aatctaactg ctgttacaag tgaaaccatt
    41761 caaaaaaatc tctctccgct tttatttgat gaagataaag aagatgagat ggcgaggaga
    41821 tttgatttgt ggttgcttca tattcagttg gggcaactga cagctaaatc ttccactgtt
    41881 catatttccc aagtgatgaa gacggctaga gctctttctg ctattggcaa tatcccgcag
    41941 gtttttgagc aggctgaaat tatggctctt tgtcaactgt agtgggttga aaaaaagcta
    42001 agcttgagaa aggacaaatt ttgtcctttc ttttttgata ttcagggcg
//
LOCUS       DAVBCB010000012        39608 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVBCB010000012 DAVBCB010000000
VERSION     DAVBCB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 39608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39608
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.012"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            470..1591
                     /gene="mnmA"
                     /locus_tag="VQU28_000616"
     CDS             470..1591
                     /gene="mnmA"
                     /locus_tag="VQU28_000616"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HET2476014.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRRLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            1732..2187
                     /locus_tag="VQU28_000617"
     CDS             1732..2187
                     /locus_tag="VQU28_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2476015.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAVVREVKEELDVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLQNIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2197..4110
                     /gene="mnmG"
                     /locus_tag="VQU28_000618"
     CDS             2197..4110
                     /gene="mnmG"
                     /locus_tag="VQU28_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HET2476016.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            4299..4388
                     /locus_tag="VQU28_000619"
                     /pseudo
     CDS             4299..4388
                     /locus_tag="VQU28_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(4534..6213)
                     /locus_tag="VQU28_000620"
     CDS             complement(4534..6213)
                     /locus_tag="VQU28_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2476017.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(6215..6448)
                     /locus_tag="VQU28_000621"
     CDS             complement(6215..6448)
                     /locus_tag="VQU28_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HET2476018.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6830..7051
                     /locus_tag="VQU28_000622"
     CDS             6830..7051
                     /locus_tag="VQU28_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476019.1"
                     /translation="MTNQVLEENYLCDSLLAFSPPRDLCRLVCILLWIKCLDALLMLG
                     FFVWGCRDSRFTTRKFHPIYQDEVVFAFF"
     gene            complement(7264..7416)
                     /gene="cibB"
                     /locus_tag="VQU28_000623"
     CDS             complement(7264..7416)
                     /gene="cibB"
                     /locus_tag="VQU28_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HET2476020.1"
                     /translation="MMKDLNNCREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(7418..7603)
                     /gene="cibA"
                     /locus_tag="VQU28_000624"
     CDS             complement(7418..7603)
                     /gene="cibA"
                     /locus_tag="VQU28_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HET2476021.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            7781..8464
                     /gene="tsaB"
                     /locus_tag="VQU28_000625"
     CDS             7781..8464
                     /gene="tsaB"
                     /locus_tag="VQU28_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HET2476022.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVGPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            8461..8898
                     /gene="rimI"
                     /locus_tag="VQU28_000626"
     CDS             8461..8898
                     /gene="rimI"
                     /locus_tag="VQU28_000626"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HET2476023.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            8888..9898
                     /gene="tsaD"
                     /locus_tag="VQU28_000627"
     CDS             8888..9898
                     /gene="tsaD"
                     /locus_tag="VQU28_000627"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HET2476024.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLYHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(9944..11244)
                     /locus_tag="VQU28_000628"
                     /pseudo
     CDS             complement(9944..11244)
                     /locus_tag="VQU28_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(11520..11871)
                     /locus_tag="VQU28_000629"
                     /pseudo
     CDS             complement(11520..11871)
                     /locus_tag="VQU28_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(11978..12270)
                     /locus_tag="VQU28_000630"
                     /pseudo
     CDS             complement(11978..12270)
                     /locus_tag="VQU28_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            12513..13719
                     /locus_tag="VQU28_000631"
                     /pseudo
     CDS             12513..13719
                     /locus_tag="VQU28_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
     gene            13712..14360
                     /locus_tag="VQU28_000632"
                     /pseudo
     CDS             13712..14360
                     /locus_tag="VQU28_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            14370..15986
                     /locus_tag="VQU28_000633"
     CDS             14370..15986
                     /locus_tag="VQU28_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476025.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTNQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLSGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            16006..16850
                     /locus_tag="VQU28_000634"
                     /pseudo
     CDS             16006..16850
                     /locus_tag="VQU28_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16918..18419
                     /locus_tag="VQU28_000635"
                     /pseudo
     CDS             16918..18419
                     /locus_tag="VQU28_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase"
     gene            18431..19180
                     /locus_tag="VQU28_000636"
     CDS             18431..19180
                     /locus_tag="VQU28_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HET2476026.1"
                     /translation="MSDNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSKLKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIRFWIKK"
     gene            19193..20365
                     /locus_tag="VQU28_000637"
     CDS             19193..20365
                     /locus_tag="VQU28_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2476027.1"
                     /translation="MNIAVIGLGHVGLAYALLFASKYKVVAYDIDSVKINNLKKGTLP
                     SKNEELMKFFCENNLNITFFDTFSEIKNNIDYYIIALPTDYDEKIGSFNTYEIEQTVS
                     KILRVKPNGKIILKSTVPIGFSNKLKRLFDTKNIIFVPEFLREGCSIYDNLYPSRIVV
                     GDETVEGRKIAELFLSISTHSTANIKNVMLVSPTEAEAIKLFSNTFLALRVAFFNELD
                     SFAERRSLNAEVVIKGVCLDPRIGNFYNNPSFGFGGYCLPKDTRQLKKEFIEINAPVI
                     EAIDISNTNRKQFIVKQILERKPKIVGIYKLGMKYNSDNYKESAILSIINELLIVGIK
                     ILVYEPNLNVSIDNVIFEKNFELFIKQSDLIVANRWDRGLEAYKDKVYTRGIWIRD"
     gene            20760..21623
                     /locus_tag="VQU28_000638"
     CDS             20760..21623
                     /locus_tag="VQU28_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2476028.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINMNFDEFGHKLNNYQESPHMRIGRRVVDCFSHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKNALHSLDKSYPLTEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRWYMKSGYLNIISELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVLWQFLKKMQIFLQTKGSNQKEIEHFIQSLQVLENPQLTALFE
                     LRLQQYKELID"
     gene            21909..22142
                     /locus_tag="VQU28_000639"
     CDS             21909..22142
                     /locus_tag="VQU28_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476029.1"
                     /translation="MLNLQFAETMELTEAELQDVRGGNLVNSMGGGGRSGISGWGVPG
                     IYPGWGNQGMSPNRGAFDWTIDLADGLFGRRRR"
     gene            22164..22841
                     /locus_tag="VQU28_000640"
     CDS             22164..22841
                     /locus_tag="VQU28_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2476030.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMVALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATSLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            22853..23518
                     /locus_tag="VQU28_000641"
     CDS             22853..23518
                     /locus_tag="VQU28_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2476031.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAILFGVSHISNHGWVLTDFFVYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            23590..24816
                     /locus_tag="VQU28_000642"
     CDS             23590..24816
                     /locus_tag="VQU28_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2476032.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFTKFLLYSIVSLSLSGQSFSLL
                     LVTFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALGTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(25167..25244)
                     /locus_tag="VQU28_000643"
                     /pseudo
     CDS             complement(25167..25244)
                     /locus_tag="VQU28_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            25315..25971
                     /locus_tag="VQU28_000644"
     CDS             25315..25971
                     /locus_tag="VQU28_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HET2476033.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            25961..26284
                     /locus_tag="VQU28_000645"
     CDS             25961..26284
                     /locus_tag="VQU28_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HET2476034.1"
                     /translation="MVSKYLLLAVIFSDLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAV
                     LRLVF"
     gene            26388..27218
                     /locus_tag="VQU28_000646"
     CDS             26388..27218
                     /locus_tag="VQU28_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2476035.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NSSPKPFNYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAV
                     NNISYTKERAEKYLYAAPTVKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQL
                     EDYNKQHSDNPTILNYTKANLQQIMGHLSDGQFDYKISDKITVETVIKNQGLDNLKVI
                     DLPSDQQPYVYPLLAKGQDELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            27259..27336
                     /locus_tag="VQU28_000647"
                     /pseudo
     CDS             27259..27336
                     /locus_tag="VQU28_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            27440..28270
                     /locus_tag="VQU28_000648"
     CDS             27440..28270
                     /locus_tag="VQU28_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2476036.1"
                     /translation="MKKIVKYSSLAALALVAAGVLAACSGGAKKEGQAASKKEIIVAT
                     NGSPKPFIYEENGELTGYEIEVVRAIFKDSDKYNVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            28424..29278
                     /locus_tag="VQU28_000649"
     CDS             28424..29278
                     /locus_tag="VQU28_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2476037.1"
                     /translation="MKIKKWLGLAALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            29378..30751
                     /locus_tag="VQU28_000650"
     CDS             29378..30751
                     /locus_tag="VQU28_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HET2476038.1"
                     /translation="MVFPSEQEQIEKFEKDHVVQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            30744..31805
                     /locus_tag="VQU28_000651"
     CDS             30744..31805
                     /locus_tag="VQU28_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HET2476039.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGGQ"
     gene            31807..32499
                     /locus_tag="VQU28_000652"
     CDS             31807..32499
                     /locus_tag="VQU28_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476040.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(32529..33098)
                     /locus_tag="VQU28_000653"
     CDS             complement(32529..33098)
                     /locus_tag="VQU28_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2476041.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADGVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            33233..33847
                     /locus_tag="VQU28_000654"
     CDS             33233..33847
                     /locus_tag="VQU28_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HET2476042.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            33849..35495
                     /locus_tag="VQU28_000655"
     CDS             33849..35495
                     /locus_tag="VQU28_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2476043.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPAGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIFHIGETVDR
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHRASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            35507..36793
                     /locus_tag="VQU28_000656"
     CDS             35507..36793
                     /locus_tag="VQU28_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476044.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGCD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            36854..37387
                     /locus_tag="VQU28_000657"
     CDS             36854..37387
                     /locus_tag="VQU28_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476045.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRTAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKASAEEEETEELA"
     gene            37465..37935
                     /gene="nrdI"
                     /locus_tag="VQU28_000658"
     CDS             37465..37935
                     /gene="nrdI"
                     /locus_tag="VQU28_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HET2476046.1"
                     /translation="MKIISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(38228..39490)
                     /locus_tag="VQU28_000659"
     CDS             complement(38228..39490)
                     /locus_tag="VQU28_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HET2476047.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFSGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT        12026 a         6862 c         8542 g        12178 t
ORIGIN      
        1 ttaggtgccc ttgcagtcaa cacaactgta gatggctata aagtcaatgc caatggtgaa
       61 tgggtttaag ccgattaaat taaatcatgt taagaacatt tgacatttta attttgaaac
      121 aaagataagg ttcgattgaa tagatttatg ttcgtattct ttaggtacct atcttatgat
      181 ttcaggaaat gtcattaaaa aaacgactcc ttttctctaa cttgaaaaat agattagaga
      241 aaatgggttg ttttatttat tatagttatt tgaatgaaga taagaagaag gtatactcac
      301 atcattcaca taatctgtat gttgactata agtttaaaaa aacaattttt aagctcttcc
      361 ttgtcttctc caaccaagcg tgttataatg aatactgctc aagcgacctt caatcgtgaa
      421 gcacacacga ccttcaatcg tgaataaacg aatagatggg agacttacca tgagtgataa
      481 ctctaaaaca cgtgttgtcg tggggatgag tggtggtgtt gattcgtcgg tgacggctct
      541 tttgctcaag gagcagggct acgatgtgat cggtatcttc atgaagaact gggatgacac
      601 agatgaaaac ggcgtctgta cggcgaccga agattacaag gatgtggttg cggtggcaga
      661 ccagattggc attccctact actctgtcaa ttttgaaaaa gagtactggg accgcgtttt
      721 tgagtatttc ctagcggaat accgtgcagg gcgcacgcca aatccggacg ttatgtgcaa
      781 caaggaaatc aagttcaagg cctttttgga ctatgccatg accttggggg cagactatgt
      841 agcgactggg cattatgctc gagtggcgcg tgatgaggac ggcactgttc acatgcttcg
      901 tggcgtggac aatggcaagg accagaccta tttcctcagc caactttcgc aagaacaact
      961 ccaaaaaacc atgttccctc tgggacattt ggaaaagcct gaagtacgca gactagcaga
     1021 agaagcaggc ctttcgactg ctaagaagaa agactcgaca gggatttgct ttatcggaga
     1081 aaagaacttt aaaaactttc tcagcaacta cctgccagct cagcctggtc gcatgatgac
     1141 tgtggatggt cgcgatatgg gcgagcatgc aggtcttatg tactatacaa tcggtcagcg
     1201 tggcggactc ggtatcggtg ggcaacacgg cggtgacaat gccccttggt tcgttgtcgg
     1261 aaaagatcta agcaagaata ttctctatgt aggacaagga ttctaccatg attcgctcat
     1321 gtcaactagc ctagaagcca gtcaagtcca ctttactcgt gaaatgccag aagagtttac
     1381 gctagaatgt acggctaaat tccgttaccg tcagcctgac tctaaggtga ccgtccatgt
     1441 caaaggagac aaggcagaag ttatctttgc ggaaccacaa cgcgcgatta caccaggaca
     1501 ggcagttgtc ttttacgatg gcgaagagtg tctaggtggc ggtttgattg acaatgctta
     1561 ccgcgatgga caagtttgtc agtacattta gattgacaaa ttttctcaat ttgctacaat
     1621 aataacagca atagaaatga tggtcaaagc tcatggatgt tgcaggcttt tttgtcctgc
     1681 acttctttgt agttttgact gtttttgtgt cgtttaaggg aaaggacaag aatgactcaa
     1741 caagactttc ggacaaaagt agacaatacg gtttttggtg ttcgggctac agccttgatt
     1801 gtccaaaatc acaagcttct agttaccaaa gacaagggca agtattacac tattggaggt
     1861 gcgattcaag tcaatgaaag cacggaagac gcggtagtcc gtgaagtgaa ggaagaactg
     1921 gatgtcaaag ctcaagctgg gcagctagct tttgtggttg aaaatcgttt tgaagtggac
     1981 ggcgtttcct atcacaatat cgagtttcat tatctggtgg atttgcttga agatgccccg
     2041 ttgaccatgc aggaagatga gaaaaggcag ccctgtgagt ggattgactt ggataagctc
     2101 cagaatatcc agctagttcc agccttttta aaaacagccc taccagattg ggaaggccaa
     2161 ctaagacaca ttcatcttga ggaataggag agaaacatga cttatcattt tactgaagaa
     2221 tacgatatta ttgtaattgg tgcgggacac gctggggttg aggcttcctt ggccgctagc
     2281 cgtatgggct gtaaggtcct gcttgcgacc atcaatattg aaatgctggc tttcatgcct
     2341 tgtaatccct ctatcggtgg ttctgctaag gggattgtcg tacgtgaagt cgatgccctc
     2401 ggtggcgaga tggccaagac cattgacaag acttacatcc agatgaagat gctcaacaca
     2461 gggaagggac cagccgttcg tgcccttcgt gcgcaggctg ataaggaact ttactctaag
     2521 gaaatgcgca agacagttga aaatcaagaa aatctgaccc ttcgtcaaac catgattgat
     2581 gagattttgg tggaagatgg caaggttgtc ggtgtgcgta cagccaccca tcaagaatat
     2641 gctgctaagg ctgttattgt gacgacaggg actgctctcc gtggggaaat tatcatcgga
     2701 gacctcaagt actcatcagg tcctaaccac agcttggctt ctattaacct agctgacaat
     2761 ctcaaggaac tgggtctcga aatcggtcgt ttcaagacag gaacccctcc acgtgtcaag
     2821 gcttcttcta tcaattacga tgtgacggaa attcagccag gagacgaagt gcctaatcat
     2881 ttctcataca cttcacgtga tgaggattat gtcaaagatc aagtgccatg ctggttgacc
     2941 tataccaatg gtaccagtca tgagattatc caaaacaacc tccaccgtgc gcctatgttt
     3001 acaggtgtgg tcaagggagt ggggcctcgt tactgtccgt cgattgaaga caagattgtg
     3061 cgctttgcgg acaaggaacg tcaccaactc ttccttgagc cagaagggcg caatactgag
     3121 gaagtctatg ttcaaggact ttcaaccagt ctgcctgagg atgtccagcg tgacttggtt
     3181 cattccatca aaggtttgga aaatgcagag atgatgcgga caggttatgc tattgagtat
     3241 gatatggtct tgcctcatca gttgcgtgcg actttggaaa ccaagaaaat ctcaggtctc
     3301 ttcactgctg gtcagacaaa tggaacatca ggttatgaag aagctgctgg ccaagggatt
     3361 atcgcgggta tcaatgcggc tctgaaaatc caaggtaaac ctgagttgat tctaaaacga
     3421 agtgacggtt atatcggggt gatgatcgac gacttggtga ccaagggaac cattgaacct
     3481 taccgtctct tgaccagtcg tgctgaatac cgtctcattc ttcgtcatga caatgctgat
     3541 atgcgcttga ctgagatggg acgcgagatt ggccttgtgg atgatgaacg ctgggctcgt
     3601 tttgaaatca agaaaaatca atttgataat gagatgaaac gcctagacag tatcaaactc
     3661 aagccagtca aggaaaccaa tgctaaggtt gaggaaatgg gcttcaagcc gttgacagat
     3721 gcggtgacag ccaaggaatt ccttcgccgt ccagaagttt cttaccaaga tgtggtggcc
     3781 ttcatcggac cagctgcaga agacttggat gacaagatta tcgaattgat tgaaacagaa
     3841 atcaagtacg aaggctatat ttccaaagcc atggatcagg ttgctaagat gaaacgtatg
     3901 gaagaaaaac gcattccagc caatatcgac tgggatgaca tcgattctat tgcgacggaa
     3961 gctcgtcaga agttcaaact catcaatcca gaaaccatcg gccaagccag ccgtatttcg
     4021 ggagtaaacc cagcagatat ttctattttg atggtgtatc tggaaggtaa aaatcgtagt
     4081 atttctaaaa ctcttcaaaa atcaaaatga tacgtcgtcg gcttcttacg aatgagttca
     4141 aagcttggct ttgattcatc tccaacctcc catagttccc cgaactatgg gagctaactc
     4201 aagttatgca ttttcctagc cttctggtct gattttcatt gagttttaat ctcaaaagaa
     4261 agcatagaga aaaacagctc cgaagacggg gctgttttgt tgacttgtac tcaatgaaaa
     4321 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcactgcttt gaggttgtag
     4381 atagaactga cgaagtcagc tcaaaacaca gttttgaggt tgtagataga actgacgaag
     4441 tcagctcaaa acacagtttt gaggttgtgg atagaactga cgaagtcagt aacatatata
     4501 cggcaaggcg aagctgacgt ggtttgaaga gtattattct tcatctggtg tgaggatcat
     4561 cgggatgatg atcggttcac gttcggtatt ttcatagagg aaggggcgaa tagcgttgac
     4621 aatggcacca ttgacagatt gcacgctagc atccttattt ttcagtgcga tacgaatggc
     4681 attgaagagg atacgctggc tttggcgaat caagtctcca gactctctca tgtagacaaa
     4741 gcctcggctg aggatatctg ggccagacag aatcatctgc gatttgaagt caacagttgc
     4801 gactgccaga acgacaccgt cttcagatag atcgcgacga tctttgagga cagctgcgcc
     4861 aatttcaccg atacgatttc catcgacata gatatcttgg gcgttgaaat gacctgcgat
     4921 acgagctgag tcagcagtaa gggcaagcac atcgccattg ctcatgataa agatattgtc
     4981 tttctcaaca ccagtatcca ctgctagtcc agcgtggact ttttgcatgc ggtattcacc
     5041 gtggacaggc atgaagtatt ttggcttaat caagcggagc atgagttttt gctcttgctg
     5101 accaccgtgt ccagatgtat ggatattgtt cactttaccg tggataactt cgacaccagc
     5161 ttcagaaatg atgttaatca gcttgttgac gctagtagtg tttccaggga tgggactaga
     5221 agagaagata acggtatcac ctggttgtaa ttgtacttga cggtgggttc cgttggcgat
     5281 acgagagagg gctgccatag gctcaccctg actacctgta cagaggataa gaacttctcc
     5341 tgcaggataa tctttgattt catttggctc gataaaggtt cccttaggag ctttgatgta
     5401 gccaagatcg attccgttga caatggcctt ttccatagaa cgaccaaaga ccgcaatctt
     5461 gcgtccagtc ttaacagcag cttctgttgc ctgctggaga cggaagatat ttgaggcaaa
     5521 ggatgcaaag atgatacgtc cttcaatacc ttggataatc ttcataatgg actgaccaac
     5581 gactttttca gagttggtaa aggttggtac ttccgcattt gtcgagtcag acaggagaca
     5641 gagcacgcct tcttcaccaa gcgcagccat acgatgcaag tccgcaggtt ctccaactgg
     5701 agtaaagtcg aacttaaagt cacccgtaca gacgattttc ccttgaggag tatgaatgac
     5761 aatccccaaa ggctctggaa tagagtgagt cgttctaaag aaagttgcct tgagattttt
     5821 aaaggtcaac tcggtgttgt ggttgatttc gtaaagtttg gcgttgcgca agaggccgtg
     5881 ttcttcgagt ttcccacgga tcaaagccaa ggcaagcggt ccagcgtaga tagggacatt
     5941 tgcttgcttg agtaggaacg gaatcccacc aatgtggtcc tcgtgtccgt gtgtgattaa
     6001 aacagccttg acgcggtcga tattgtctac gatgtaagag tagtcaggaa tgacatagtc
     6061 gataccaagc aagtcatctt ctgggaattt aatcccagca tcgacgatga taatctcgtc
     6121 ttggtattca attccgtaag tgtttttccc gatttctcct agaccaccga tggcaaaaac
     6181 accgacttct tcaggtttaa gagtgtaggc catattagaa ctccgtaatt tcgaaggcgc
     6241 cagtttcttt ttcgtaatcg agcaatttgt cagacaagag ttcgatatat tcgatattgt
     6301 actctgggcg attttcttcg acaagttggc gagcagtgat acggccctca agttctgagc
     6361 tggcatcgat gtctaggtaa agcgcgcgtg ttgtttcacg gcgtgggcta cgttcttttg
     6421 tttcttgata aaaaactttg taaatcatat agttcctttc tatttacagg tcagcttatt
     6481 ccaaactttt agcagatatt tgcttgattt cattccattt tgtgtctaga ttgaccttga
     6541 ttcgttacaa ttatttcaat tataccacaa aatgaaaaat tagtaaaaga cggaaacgag
     6601 aaagttaggg gacgatttct tcggtaagcg tttgcaaaaa caaagaaaat gtgttatgtt
     6661 gataacggaa gtgaagttta ttacttgtca taattccata tagattttaa gtcaaggagg
     6721 ttgccctatg tataacttat ttgcttttgc tatcggagtt cgtttgctag cttttatcgt
     6781 ttttgttttg cgtttatgga ggaaatcttc tatttgccaa actggaacga tgacaaacca
     6841 agttcttgag gaaaattacc tttgtgactc tctactcgct ttttctcctc ctcgtgacct
     6901 atgtcgtttg gtttgtattt tactttggat taaatgctta gatgccctgc ttatgctggg
     6961 gttttttgtt tggggatgta gagatagtag atttacgaca cgaaaattcc accccatcta
     7021 tcaagatgag gtggtctttg catttttcta aaatgatatg acaatggaat tttcaaagct
     7081 tattattttt atgggagtca ttgttctgct caaaaataga gttgaagcat aaaaataaaa
     7141 caatacaaac agataaatcg aatggtaaag ggatggtagt aatgttcaaa tcattgagaa
     7201 tgctgagcaa actgtaagag atgaagacaa taaaatttat ccttctaaaa atgtgtttct
     7261 tatctagcat gacttaccaa actttttacg aaggtctttt ccaatggtat aacctgccat
     7321 aaataaagcg ataccaacac ctacaccaaa gccacccttg atttcttgca attccttatt
     7381 agaaatttca cgacagttgt tcaaatcttt catcatatta atctcctcct aaaaaataac
     7441 cagctgccaa accagaacct ataagtgctg ttccacctgc aatagctttc caagatactg
     7501 ctactccaaa gataaccaag ggagcgagac cgccatcaat atctgataat tcattttcag
     7561 ataaggataa aaattgattg tcaagaatgt caaaatttgt cattgaactt tcctcctttt
     7621 ttcttgttca cttttatatt cggaaaagaa aaggaaaagt gagcattttt ttaaattatt
     7681 tttagttaag tgagggcact cttgtctggg ggcaaagaaa attcccatgt gccatctttt
     7741 gtagtataat agtaagcaga caaaaagata ggaatgtgtt atgaaagtat tagcttttga
     7801 tacgtccagc aaggctcttt ctctcgctat tttagaggat aagcaggttc ttgccgagac
     7861 gacgattaat atcaagaaaa atcacagtat taccctcatg ccagccattg attttttgat
     7921 ggagagtttg gactggacgc ccaaggattt ggaccgaatc gtggtggcgg aagggccagg
     7981 tagctacaca ggcttgcgaa tcgcggtagc cactgccaag accttgactc atactctgaa
     8041 cattgagtta gttggtatgt ctagcctctt ggctctggtg ccatatcaac aagaaggctt
     8101 gttcgttcct ttgatggatg cgcgtcgcaa caatgtttat gcaggatttt atgaaaatgc
     8161 taaacctgtc atgccagaag cgcacctatc ttttgaagag gtgctagaaa aagtcaaggg
     8221 tgctagtcag gtaacctttg tcggagaagt tggccccttt gtggagcaga ttcaagaaca
     8281 cttgccaagg actaattata aagaaacctt gcctaatgca gccaatcttg ctcttttagc
     8341 ttgggacaag gaagcagact ccttgcatga ttttgtgcca aattacctca aacgagtcga
     8401 ggctgaggaa aactggctca agaaccatac cgagtctggt gagtcctaca ttaaacgcct
     8461 atgatagaaa tcaagcgaat tcaacaacag cctgacctag ctcaagccat ctacgctgtt
     8521 atggcagctg tttacctagt cagtccttgg actctggagc aaatccaagc agatctgtcc
     8581 caagaccaga cttggtatgc attggcttat gatggggcag aagtgattgg atttctagct
     8641 gtgcaggaga atctttttga agcagaagtc ctgcaaatcg ctgtcaaagg agcttatcag
     8701 ggtcagggaa ttgcgtcagc cttgtttgct caattgccga cagataagga gattttcctc
     8761 gaagtcagaa agtcaaatca acgagcgcaa gcattttaca agaaagaaaa aatggcggtc
     8821 atcgctgagc gaaaggccta ctaccatgac ccagtcgagg acgccattat catgaagaga
     8881 gaaatagatg aaggatagat atattttagc atttgagaca tcctgtgatg agaccagtgt
     8941 cgccgtcttg aaaaacgacg atgagctctt gtccaatgtc attgctagtc aaattgagag
     9001 tcacaaacgt tttggtggcg tagtgcccga agtagccagt cgtcaccatg tcgaggtcat
     9061 tacagcctgt atcgaggagg cattggcaga agcagggatt accgaagagg acgtgacagc
     9121 tgttgcggtt acctacggac caggcttggt cggagccttg ctagttggtt tgtcagctgc
     9181 caaggccttt gcttgggctc acggacttcc actgattcct gttaatcaca tggctgggca
     9241 cctcatggca gctcagagtg tggagccttt ggagtttccc ttgctagccc tcttggtcag
     9301 cggcggacac acagagttgg tttatgtttc ggaggcagga gattataaga ttgttgggga
     9361 aacccgtgat gatgcggttg gtgaggctta tgataaggtc ggccgtgtca tgggcttgac
     9421 ctatcctgca ggtcgtgaga ttgacgagct ggctcatcag gggcaggata tttatgattt
     9481 cccccgtgcc atgattaagg aagataatct ggagttctcc ttctcaggtt tgaaatctgc
     9541 ctttatcaat ctttatcaca atgccgagca aaagggagaa agcctgtcta cagaagattt
     9601 gtgtgcttcc ttccaagcag cagttatgga cattctcatg gcaaaaacca agaaggcttt
     9661 ggagaaatat cctgttaaaa ccctggttgt ggcaggtggt gtggcagcca ataaaggtct
     9721 cagagaacgc ctagcagccg aaatcacaga tgtcaaggtt atcatccccc ctctgcgact
     9781 ctgcggagac aatgcaggta tgattgccta tgccagcgtc agcgagtgga acaaagaaaa
     9841 cttcgcaggc tgggacctca atgccaaacc aagtcttgcc tttgatacca tggaataaag
     9901 agtggctctt tgtcaagtgt agtgggtagt cgaaaagctg caatctggag agtacgaaat
     9961 tcgtactctc ttttttgatg ttcaaagtca tcaaaatccg ttttttaaaa ttgttaaagt
    10021 tccggaaacc aaaggctttg cgcttgataa tcttaatcaa attattggtc gcttcaagtt
    10081 tagcgttgga ataaggcagc ttaagtgcgt tgataatctt atctctatcc cttaaaaaag
    10141 tccaaaagac cgtttgaaaa agcggatgaa ccgttggcag ttcttgctca atcaatccaa
    10201 agaaatggtc ggcattcttc tcttgaaaat gaaagagcag gagttgatag agttcgtagt
    10261 gaacctgtaa tccctcagag taagaaagca gcttatctcg tacttcccga tgggttaagt
    10321 gcattcgaaa cgtgggctga tagaatcgtt tctgacagag cttgaagcta tactggtgta
    10381 gtaaatcagt ccagtggact gatttagccc gagcctagaa acgaaaaagc gagggttcca
    10441 aaagcgcttg agcgcccgat actccaagga ttttcggtca aattggttca tgatttggat
    10501 tcgtactcgg ttcatagctc ggctcagatg ttggacaatg tggaagcggt caagaacaat
    10561 cttcgccttt ggaaagagtt gcttagcgag ccgataataa gggctgtaca tgtccatggt
    10621 gatgacctcg accagctccc gaacctctct ctgatagcgt tggaaatggt ttcgaatcac
    10681 cgcatgagtt cgcccgtcta aaattgcgag gatggatttg gactcaaaat cttgggcaat
    10741 gaagctcatt ttgctctttt tgaagctata ctcatcccaa ctcataactt ttggcaactt
    10801 ggtccaatcg gtttcaaact taaattccct cagttttcgg atgacggtgg aagttgagac
    10861 cgccaatctg tgggcaatct cagtcataga ctgcttttca aggaggagct gagcgatttt
    10921 ttgtctcacc atgttggaaa tctggcaatt tttcttgaca atggatgttt gagaaacgac
    10981 caccttaagg caattcttgc actgaaagcg gcgctttttg agatgcagta ccgtgggtaa
    11041 accctgacag tcgagaagcg gaattttaga ggctttctgg aagtcgtatt tgatcatctt
    11101 cccttgacaa tgaggacaag gaggagcggg ggaatccaac tttgcctgaa cgactagatg
    11161 ggtttggtat ttcagaacag acaagatttt gatatttttg tcttccaatc cgagcaaatc
    11221 tgtggtattc ttaagttgtt ccatatggac cctttctaat gagttggtta gacgcttttc
    11281 attataggtc atatggggct ttttttctac aagaaacgac cctataattc ctggggtggg
    11341 attacccact acagaaatta tagagccaaa gcattccaaa gtcttgtctg actctggaat
    11401 gttttctgtt cacagttgtt ttgtaactgc ctaataagta accgcgctat aacgaggtat
    11461 ctaccgagat tgtcaattct ccagtatact gttataaaaa acaaaactgg aggatttttt
    11521 tatgtcctcc catcgtccca tcgactgttc cgtagctgcc caataacgga gtatagtcgg
    11581 ctgaaacaag aataggacaa aagtgcctca agaaaagcat cgtaatttga caatattatt
    11641 ctgagatgtt tttgatgtaa gcccatattt tctcaatggg attatactcg ggtgaatagg
    11701 taggaagtgg taacagtcta tgccctgctc cttacacatg ttcattctgt gaaaccttgc
    11761 attgtccata atgataaggg atggtgtatc taaagtaggt agtaagaatt tttggaagca
    11821 agcttcgaaa aagtcactcg tcatcgtatc tttataggtc atcggagcca tagtatattg
    11881 aaacaaaaaa gggcattagt acttctataa cagtatcgca atctctggtg tgcgttgtct
    11941 cgatcttaat tcaaataatt atatcttctt ccagagagac taactgcctg ctcaaggctc
    12001 gatcatattt gagataaaaa taggtctcaa cccctgtctc ataaatataa ataggagtca
    12061 ggtagcttaa gtgattcaat tctttaagga accgatttac tttttctggg tcttgttcgc
    12121 agtaggtacg gctcttttta gactatatcc catagccttt agagcgtaat gaatagttgt
    12181 tggaggacag ttgaattcag cagctatttc agtcaaataa gcatcgggat gagtttcaat
    12241 atagatcttt aatttatctc tatcaacttt ttcaattaat agtggaagtt ttcttaaagt
    12301 tagtgtgttg aaacgttttt atacagtgct aaaatttgaa gtgtagtgca gatgtatttg
    12361 taatagtatt atagcaattc tttggagaag gtcgagacaa gagaaaatag gtatctttga
    12421 gatataagta tgatttacta taaaattatt gagttattca taaaaatttc tctatcaaat
    12481 atcataatgc gtcgtttaag gagatggtta caatgcaata tagttgtgga aaaataaata
    12541 ttaatatccc tgatggatat ggagacatta aggatatagt gttttcggct catattatag
    12601 ttaggtataa taatggacat tgtggaggta tagatccgca tataattgga ctttgtaaaa
    12661 agcagatcag aaggatgtct ccatatccta ttttgataat cgtatctaga gattcaaagg
    12721 ttattgatga ttataaaaat ttagatattg cctatgttga ttgtactcaa tgttcaaata
    12781 attttgaaac tgccttacac gttaaaaata ttttaaaatt attaaaaatc caacttatac
    12841 actgtcatgg ctactcaact aattattttt tatatatgtt aaaaaaactt gacaagaatg
    12901 gatttggaaa ggttaagaca gtgataacat gccatggatg ggttgaatat aacttaaaaa
    12961 agaaattctt gacttatttt gatttttgga catattctat gggagatgct tttatttgtg
    13021 tatctgaaac tatgaaaaaa agattggaga gtataataaa aaaataagaa gatagttgca
    13081 ataaataacg gaattaatgt atcaaatagc gatttagatg tagtaggtgt tcaagacttt
    13141 aaaaaagaat tttgtattcc aaataacaaa aaaatcattt gttatgttgg aaggttggat
    13201 ccagaaaaaa ggccggatag attccttgaa tttgcagaaa aattattttt agtcagagaa
    13261 gatgtgattt ttattatggc tggaaatggt agtatgtggg ctactttgaa agaaaaaatt
    13321 tgccatttga aatgtagaga taatttcagg ttacttggtg aaatctatcc agctactata
    13381 gtttaccaaa tatcggattt attgtatatt ccttctgata cagaaggaat acctatgtgt
    13441 gttttagagt caatgtcaca agaaactccg gttttagcta gtaatgttgg cgggttaagt
    13501 gaaattattg aacatagggt tgatggattt ttatttgaga aggaagatgt tgagggagtg
    13561 tgtgcttgtg ctaatttttt actcaatgat tctgagtatt tgaaatatat aggtgagaat
    13621 agtaaatcaa aaataagaaa acatttttct gtgcaaaaaa tgtttgtaga aaccatgaga
    13681 gtatatgatg aattattaga gaagagtagt catggatagg aatatagatc aagaattagt
    13741 aagtattata ataccaacac ataataggta tgaatctctg attagagctg tgaaaagttg
    13801 tttacatcaa agttataaaa atatcgaagt tataattatt gatgataatt atagtaatgt
    13861 aaatttaaga aataaaatta tccatcaatt tggctatacc agtcatagaa ttaagttaat
    13921 tttaagtaat gaagatttag gtgcaactaa tgccagaaac ataggtatca aaaattctag
    13981 aggtaagtat atatcatttt tagacgatga tgatgaatat atgccagatc gaattttaaa
    14041 gttgatggct tgttttaaaa agagtagaat gaagaattta gctttagttt atagttatgg
    14101 aataataatt tatccaatag gaaatatttt aatttattga gtttcaacga gagaatattg
    14161 gttacaaaga aatattattc tttaaacata aagcggttac tattaatagg agacaaatgc
    14221 aaggctttaa aagtaatcaa gaaggcaaga gaagaaaaaa tttttaacga atttcttttt
    14281 cgaaatatat gttattatat cattttggtt ttttctattg tatatatgac aactatgttc
    14341 aattaaaatt tagaaagtga gaaactattg tgtatactat tataaaatca aatataaaaa
    14401 aatttagttt attaacgata tttattgttg ctggtcaatt attgctaatt tatgcagcaa
    14461 ctattaatgc tctggtgttg aatgaattaa ttgcgatgaa tttagagcgg tttttgaaat
    14521 tgtcaatcta ccaaatgatt gtctggtgtg ggataatatt ccttgactgg gtagtgaaaa
    14581 attatcaggt tgaagtgatc caagagttta atctagagat tcgaaataga gttgccacag
    14641 acatctctaa ctctacctat caagaatttc atagtaaatc atcaggaaca tatctttcgt
    14701 ggctaaataa tgatgttcag actttaaatg atcaggcttt taaacaactt tttttagtaa
    14761 taaaaggaat ttctggtact atatttgcag ttgtgactct taatcactat cattggtcat
    14821 tgactgtagc caccttgttt tcattaatga ttatgctact tgtaccaaaa atctttgcat
    14881 cgaaaatgcg agaagttagt ctaaatttaa ctaaccaaaa tgaagctttt ttaaaatcta
    14941 gtgagactat attgaatgga tttgatgtgt tagcgtcctt gaatctttta tatgtattgc
    15001 ctaagaaaat taaagaagca ggaattttat taaagatggt tatacaaaga aagacaactg
    15061 tagaaacgtt agcaggcgct attagcttct ttctcaatat tttttttcag atatctctcg
    15121 tttttttaac aggctatctt gcaataaaag gaatagtgaa aattggtact attgaagcaa
    15181 taggagcact aacaggtgtt atttttacag cgctaggtga attaggaggt caattatcct
    15241 ctattattgg tacgaagcct atttttttaa aattgtattc aattaatcca attgagtcaa
    15301 ataaaatgaa tgatatcgaa ccaaatgagg tgaatagaga ttttccgtta tatgaagcaa
    15361 aaaatatttg ctataagtat ggagataaag aaatattaaa aaacttaaat ttttgttttc
    15421 aacgtaatga aaagtattta attttaggtg aaagtggaag cgggaaatct acattattaa
    15481 aattattgag tggctttttg agagattata gtggagaatt gcgattctgc ggggatgata
    15541 taaaaaaaac ctcctattta aatatggttt cgaatgttct atatgtagat caaaaagctt
    15601 atttgtttga aggtacgatt agagataata ttttattgga agaaaattat actgatgaag
    15661 aaatactaca gtctttagag caagttggtt tgagtgtaaa agattttcct aataacattt
    15721 tagattatta tgttggtgat gatgggagat tactgtcagg agggcagaaa caaaaaatta
    15781 ctttagctag agggctaatt agaaataaga aaatagtatt aattgacgag ggaacttctg
    15841 ctatcgatag gagaacttcg ttagcgattg aacgtaagat attagataga gaggatttga
    15901 ctgtcattat tgttacccat gctccgcatc cggaacttaa acaatatttt actaagatat
    15961 atcaatttcc aaaggatttt atttaagaat ataggggagg gaaacatgag attctattgt
    16021 gaagcttatg aggtaattac tgaagagaaa aaagtatact ttaatgatat tgagttagaa
    16081 gttaagtatt cctctgtcga agaattgttt ataatatgtg agaaattatt agataagaaa
    16141 aaagtatcat ttttttatgt atatgagaaa cccttaaggt atctgttatt tgactatata
    16201 tttctactag ttctagctaa aaaaaatatc ccaatcttag atggagtttc caataagcaa
    16261 gttgatccaa cgttgctgca tcacttttct ttggaaattg aaaagaattt tatcgatttt
    16321 tgctataaaa atatggatct catcctaaaa actcaaagta tatctttgtg tcatagagaa
    16381 gagcttatta ttgtagatgt ttcagatgac tctaaatttg gtgtttttta caagcgtttt
    16441 agaacactgg ttgataaaaa taacggaaaa tatgtttgtg tttataattt cagtcgtgtt
    16501 tcagatatac tgcaaaactg gaaaaattat tgcaatcgaa aattttcggt tacgtttact
    16561 gaaagccaat ttgaattatt taagctttta tataatcaaa aaaattttaa aacaatttcg
    16621 ttgctatttg gaaaaaaatt gttgctggag gaattatcta ttatagcgat cttactaata
    16681 tagaatactt ttgtattttt tggtgggata gtatgtttgg gaaagattct attggaaaat
    16741 acgtatatgt agaagagatt agtagatgtc attttttaga tagaaattat tctttttgtt
    16801 atggtttaca agattataaa agtaaactta ttaaatattt tttagaatag ttaatctatt
    16861 cgagagagat aaagaaatat ggaaaatact ttattgaata tcacaggaag cttcgatatg
    16921 gaaactagga atctaattag ttattcttta actgatatct ttgaaacaga taaaataaga
    16981 attgaacttc ttggagaaat ttattataaa aatataaagt tggaattgca tgaattcgct
    17041 ggattgtata agatttatgg aattagctta ataaagaata ttaccggaat gtttttaatt
    17101 ataatttttg atacgaagac aaaagaatta aaaatttttc aagatataac gacttcatac
    17161 tttaatttat actatactgt ttatggaggt gtgttctata tatagtacat ctttaaagaa
    17221 agtgatgaag ttatctcatg ttcccgttac cttaaataac aaaaaaatac aagaatttat
    17281 gagaaatgga tttattttag attcaaatac tttagtaacc gaaattaata aattagaata
    17341 tttctcttac atttctgtaa ataatactct tcgtatatgt ggtattgatt ataatgattc
    17401 aaacaatttt actaaagaac aagtattaaa gaattgggac tctatgctac gtgaatctat
    17461 attaagagtg tatagtgaag caggagaggc taatattaca ctatcatctg gttttgattc
    17521 aaattatata ttatatacat tagcgaatta tacgaattct tcaattaatg cattttgcat
    17581 aggaggagaa aaaggaatta atgagattcc tgaagtaact aaaattgcaa aattttatgg
    17641 tataaattta ttagttgata ctgtaatgag cccaagattt agaatatttt cccgatatag
    17701 tatggagatt ggaaggaagt ctctttgaat gtggtgtcat actgcagtac tatttaggca
    17761 ggttgttgtt ccaaaaaggg aacacaagta ttctttgtgg cgagtcagcg gatgaaataa
    17821 tgacttttaa gtatcattct gtgaactaca atcaattttg taatgataaa cagaagtcag
    17881 tatattttag ttattccgac tatccatttt atgttacaaa ttcaattgtt ttaaagaaaa
    17941 atagcctact tttgcatagt tttggcattc atcctagata tccatataaa atgtctgaga
    18001 ttgttgaaat gtctaaaaaa atatcagatc taaacgataa aaaagaattt cacaaaaaga
    18061 actgtgagtt gaggtttcaa gatagtgttc ttgataacat aaatagtgtc ccgggtacaa
    18121 ctcatttatt tagttgttta aatattcaaa cattagtaaa aattatttta tatatttttc
    18181 gatacaacag ttgtatgaaa atatttaatt ttagagataa agagcttatc ttttttaata
    18241 agattgtaaa tgggctgata caaaatattg aggagaattt agaggatgat attgaaagga
    18301 tattgaaata tttatatata tgtttattta acgaaatatt cattattaag aataaggtga
    18361 atttttttga tgatgtggaa tttaatcaga cacttagtga gtttttagat aaattgtaga
    18421 gggaatggtt atgagtgaca atactaagta tatcttttta tctccacatt tagatgacgc
    18481 aattttttcc tgtggtgatt atatttcaaa attaacttca gaaggagaaa ttgtacttgt
    18541 tattactata ttttctggtt accctttgag ccaacagcta cagccctctg ctaagcaatt
    18601 tcataagttg tgtaaccttg ggaaatatcc gattgaagaa aggaagaaag aagacaggtt
    18661 agcttgtgaa cgtctacaat gtgattttag acacttatcg tactatgaat gtctctatag
    18721 gaaagataga aatggcaatt ttctataccg tcatatctat agtaaattaa aaaatgagga
    18781 cacattaaag aatgatatca taaaagagct gttaatgcat ttagacgaca aatgtgtcgt
    18841 atattgtccg ctttcacttg gtgaccatgt tgatcatgtt tttgtgaata gtattggaag
    18901 ggcgcttgaa tttatgcgtt ataaagtaat atattatgag gacttcccat atgtaagtga
    18961 tagtagtatg gttagttata tgggaaaaac taaagaatta aaaatgtatc aagaggaact
    19021 tgatgaaaaa cattatattg atagaatatc atcaatattg tgctataaat ctcagatttt
    19081 gataatctgg aaatcagttg aaaaactttt aaacaatatt aaagagttgt atttaaggaa
    19141 tggggcagct tattctatta gattttggat taagaaataa ggaagtagtt ttatgaatat
    19201 agcagtaatt ggtttggggc atgttgggct ggcctacgcg ttactatttg catctaaata
    19261 taaagttgtt gcatatgata tagattctgt aaaaataaat aatttaaaaa agggcactct
    19321 tccatctaaa aatgaagagc ttatgaagtt tttttgcgag aataacttaa atattacttt
    19381 ttttgataca ttttctgaaa ttaaaaataa tattgattat tatattattg cgcttccgac
    19441 agattatgat gagaaaattg gtagttttaa tacatatgaa atcgaacaaa cggtatcgaa
    19501 gattctgagg gtaaaaccta atggaaagat tattttaaag tcaacagttc cgatcggctt
    19561 ttcaaacaaa ttaaaaaggc tgtttgatac aaaaaatatc atttttgtcc ctgaattttt
    19621 gagagaaggt tgttctatat atgataattt atatccaagt cgcatagttg ttggagatga
    19681 gacagttgaa ggaagaaaaa ttgcagagtt gttcctttcg attagtactc atagtactgc
    19741 caatattaaa aatgttatgt tagtttctcc tactgaagca gaagcaatta agcttttttc
    19801 taacacattc ttagctctcc gtgttgcttt ttttaatgaa ctagattctt ttgctgagag
    19861 gagaagttta aatgctgaag ttgtaataaa aggtgtttgt ttagatccaa gaattggaaa
    19921 tttttataat aatccttctt ttggatttgg aggatattgt cttcccaaag atactagaca
    19981 attaaaaaaa gaatttatag aaataaatgc cccagtgata gaagcgattg atatttcaaa
    20041 cacaaataga aaacagttca tagttaaaca aatattggaa cgaaagccaa agatagtagg
    20101 aatatataaa ttggggatga aatataattc agataattac aaagagtcag ctattttaag
    20161 tataattaat gaactattga ttgtcggtat aaaaatttta gtatatgaac caaatttaaa
    20221 cgttagtata gataacgtaa tttttgaaaa gaattttgaa ctatttataa aacaaagtga
    20281 tctaattgtt gcaaatagat gggatagggg ccttgaagct tataaagata aagtttacac
    20341 aagggggatc tggataagag attaactatt gatattaaaa aggcaataat ttataaaatt
    20401 tgtacaaaaa aatgttaaat tttcacattt tcatagtata ctgtttaata gaaagtaggt
    20461 tctttgtaac cgctccatta taaaatcctt tcactcttcc ggtatactat taggaaaaac
    20521 ataactggag gatattttga gctcacacct catcgttcca gtggctgttc cccaagctcg
    20581 tgtactgccc aaaaaagtta gacattctat ttagaacgag gattgagttc tgtattgtac
    20641 aaggctcggt ccttttagag tcagcttaag gctggctttt tcaatcacca aagtgtcaga
    20701 atgttttgac aaatgaacac aaataatgat ataatatgca aaagctagga ggtggtagga
    20761 tgattgaaaa aatggaactg ggggaatttt acaaggaatt gcgcttggcg agaaagctca
    20821 agcagtcaga tgtggcttgt gctggactaa cagcctctca gttgtccaag tttgaactag
    20881 gacagtctat gctgtctgca gacaagctga tcctagccat ccaagggatc aatatgaatt
    20941 ttgatgagtt tgggcacaaa ctcaacaact atcaagaatc tccacatatg cgaattggta
    21001 gaagggttgt ggattgcttt tcccatcagg atatagctgc tttagagcag ctattggagg
    21061 aagtcgatca agaacagatg gcgcagacct atcgtcgttt gaatgccatt gtgattaaaa
    21121 atgcactcca ttccttagat aaaagctacc cgctaacaga ggaggatagc gagtttttga
    21181 ctacctacct ctacgctatt gagtcttgga cctggtttga actctatctt ttttgcaata
    21241 ctatgccctt cttgagcaat caagatctga tctttttatc aacctcctta ctcgaaaaat
    21301 ccaaggaatt taaagagtta gtacacaatc gatggtatat gaaaagtggt tatcttaata
    21361 tcatctcaga acttatggag cgcaagctat tttcttatat tccaatcttt gaagctgagc
    21421 tggagcgcat gctccgtcca tacgatgttt ttgaaaaagt attgtggcag tttttaaaga
    21481 aaatgcagat ttttcttcaa accaaaggaa gcaatcaaaa agagattgaa cactttatcc
    21541 aatctctgca agtattagaa aatccacaat tgacagccct ttttgaattg cgtttgcagc
    21601 aatacaaaga gcttatcgat tagttagaaa tgggaaaaag ataataatat cagcaacgat
    21661 cgtcaataat gttaaaactt gtttcttcat attacttccc tcctcattag taataacttt
    21721 attataccag ggaaataatc aaatctacca aaatcgcaaa tctgaaattt ctataagaaa
    21781 aaatatcaaa tatgcgattt tctaaaataa gccaatttcc gtgttatact gtccttgtaa
    21841 aacatttgaa gcaaatccta ggtcgcagaa gtggtttaca aatgaagata attgaaggag
    21901 tgtaaaagat gttaaattta caatttgcag aaacaatgga attgacagaa gctgagttgc
    21961 aagatgttag aggaggcaac ctcgtaaatt ctatgggagg tggtggaaga agtggtatat
    22021 ctggttgggg cgtcccagga atttaccctg gttggggcaa tcaagggatg agtcctaatc
    22081 gtggagcttt tgattggact atagatttag cagatggatt gtttggccgc cgtagaagat
    22141 aggctcttat aggagataat aggatgaaga aaatgaaaga agtgaaattt cacctagcaa
    22201 ctggcttgtt gattttgact tattacctca tttttaatgt cacatcagac ttagatttta
    22261 tggttgcttt gtctgataat atgtactatg tgttccaagt tttattggta cttattctgg
    22321 gaactatagc tacaattgct tttgtcaaaa gtgaacattg gaaagagtgt ggtcgtttcc
    22381 agtttcgttg gtcttacctg ggtgttttcc ttctttcttt tttcttattg ttcgtgtggg
    22441 caaatctaac aacctatatc tttcctcgta cccagaatgg aagtacagtc gtagaagtag
    22501 ccaccagttt aacaggaatt agctattttg ttacgcgaat actttatact agtattatcg
    22561 cacctgtatc agaagaggtt gtttgtcgtg gtcttttgat gacaagttta tctaaagtta
    22621 agagatacta tcttgatgtt cttgtttccg ctgccatatt tggcgctatg catgttttgc
    22681 agtatggttg gataacgaca gatttcataa agtattttgg aatgggcttg atattttgta
    22741 tgatgtttcg ctacacccga tccatctatt gggctatagc tctgcatgct tcatggaata
    22801 gcttcttact cattgttact ttattggtat ttggatatta gaaaggtgat atatgaaaaa
    22861 gataatctca caccgctact ttattatagt ttttctacta gttattgctg accagaagtt
    22921 cagtgtttta gttttacgca gcgaccttgt tgctgggcta tctgactttg cctattatct
    22981 gtcggatatg atgttgaatt ttcttgtggt tttatttgct cttattgcta tgatttggtc
    23041 ggggaaatgg cagaaaatca atagtagaaa gtttaaagga tcctatcttt tctattcctt
    23101 cctagctctg cttgcttttg ttgcttggaa ttttgtcacc ttttttcttt tcccacctac
    23161 tcgaaatgaa atttcttatc aacatgctgc tcctactttt acaggagcta cggcattttt
    23221 gatgtatttt ttttatcctg tgattgcagg tcccattttt gaagacatga tttatcgtgg
    23281 attggtgatg acagctctgg aaaaaggaaa gaaatggggg ctagatgtac ttggttctgc
    23341 tattttattt ggggtctcgc acattagtaa tcacggttgg gtcttgacag actttttcgt
    23401 ctatatgggt ggcggactta tatttgcagt cttatttaga atgacaaagt ccatttattg
    23461 gcctattggg ctgcatatag tctacaacgg cattggtcag cttttgatgt tactgtaatt
    23521 ttggttgtta gtagtatcgc ggtctatttc tggggaaaga ttcccatttg aacttaagaa
    23581 ggagttgtga tgaaagtatt tcttcaaaat agagatttta ggcaattaac tatcaaccag
    23641 tggatttcaa cgcttgggga tacgattttt tatctggcct tcctgaatta tgtggcagat
    23701 gcgtcttttg cccctttggc gattttactt atcacgattt cagaaaccct tccccaagtt
    23761 ctacaaatct ttctgggagt tttggcggat tttcaacatc atcgtgtctt gaaatacaca
    23821 gtcattagtt ttacaaaatt tttgctttac tctatagtgt ccctatcgct ttcagggcag
    23881 tctttttcct tgttgctagt aacctttatt tgtctgctta acctcttgtc tgacacattg
    23941 agttattttt caggcgccat gctcactccg attttcatta gaattattgg gcaagaccat
    24001 ctggcagaag ctattggatt taaacagtca actgttagtt tagtgaaaac aatcagtaat
    24061 atcctaggag gagtcttact aggcattcta tccatccagt ttatttcctt actgaacgct
    24121 cttacctttt taatcgcatt tttaggtatc ctcttcataa aaactgacct cttgaaagta
    24181 gaaaaaacga ttagctatca agaaggactc tctgtaaaat ccttttgcca gcatttgctc
    24241 caatcatcga aattgatatg gaatatgaat aaggtgctct tggttttgtt tattatctct
    24301 actagtcaag cagtgataaa tgttacggtt cctatttcca ctctattttt aaggaaccag
    24361 ccctttttga atttacaaac tggtcaatct cttgccttgt tagccacact agaattatca
    24421 gcccttattt tgggaagtct gatgagtggc tatctgcaac ataccatttc cataaaaata
    24481 gccctgtatg cttcactcct actccaatta ctactcctag tgggctttgc gacagctcat
    24541 tttggcttga ttctgatctt tagctttttg gatgcctttt tcgcgggtat tctctctcct
    24601 agattgcaag aactcgtctt taaacaaata cctgaggagt caatgggagc agttcaatct
    24661 tctatcggtg ccattacggt tgttttacct agcttattta caatagcttt gggaaccatt
    24721 gctactagct ttggaactct ggcagttagc tttgttttat tactatttct tctagttgcc
    24781 tttgtcatgc ttctgaatat tcgtgaaagt atttagtgga gaatttctat aatatatgtt
    24841 tgagactgga ttgccagtct ttttatgatg gtcaaaatca acagactttt tctcttttct
    24901 gaatgtgtta tagtagactg aatctaaaat agtacgaaat aattgctaaa acatttatag
    24961 aaattaattt tactttccca atcgatttgt tctcatctta tttcaatctg ctatataata
    25021 gtccatgctg tggctggatt cttttcagcc tacttatact cttcgaaact ctcttcaaac
    25081 cacgtcagct tcaccttgcc gtaggtatgg ttactgactt cgtcagttct atccacaacc
    25141 tcaaaacagt gttttgagct gacttcgtca gttctatctg caacctcaaa acaatgtttt
    25201 gagcaacctg cggctagttt cctagtttgc tctttgattt tcattgagta ttattaagac
    25261 aatatgagga gaaagatgaa agaaaaagga ttttaggagg gggcgcaggc agccatgcca
    25321 acggctcttg gctatgtcag tatcggcctg gcctgtggga ctatcggtgc gccctatgtg
    25381 acacctgttg agatgggctt gatgagtctc tttgtttatg ctgggagtgc ccagtttgcc
    25441 atgttggcac tgattgtggt tcaagctcct gtggcagcta ttgctatgac ggtttttcta
    25501 atcaacttgc gtctcttttt gttgagttta cacgcatcga cttatttccg tcataccagt
    25561 ctttggtaca atatcggtat gtctagtatc ttgacagatg agacctatgg cgttttgatg
    25621 ggtgaattgg cccatacaga caaggtaaat cctatgtgga tgcacggaaa caatcttaac
    25681 agctatgtgg cttggtttgt ggggacagta gtcggaacgg ctctgggtgg cctgctacca
    25741 aatccagaaa tctttggctt ggattttgcc ctggttggga tgtttattgg tatttttgct
    25801 tcgcaatttc agattatgca aagacggatt cctgtccgca atctgctcat tatcctagca
    25861 gttgttgcgg tgtccttctt tttgctcttg acagtggtgt ctcagtcact agctgttctg
    25921 tttgcgacgc tacttggttg tagcatgggg gtggttttag atggtcagta agtatctttt
    25981 attagcagtt attttctctg acttggtgac ttggattctc cgtatgattc ccttcatctt
    26041 agtcaagtat aagggcttgc ctgcaatcgt tgagcgtttt ttgaagttct tgcccgtttc
    26101 cattatcttt gccttgattc tttcaagcgt agtgacaggt aaggttggga gccttcctca
    26161 aattaaatgg ctagacttct tagccgtctt tccaacagct tgggtagcct ttcgctaccg
    26221 caatctagtc ggaacagttc tctttggagt agtcttgatt gccgtcttgc gtttggtctt
    26281 ttaaatcagc cataaagaaa acctatcaca gatatatata tcatataatg gtgtaaatga
    26341 tttttttctg ttaagattat aaggtattct attttggagg aaatgacatg aaaaaaatcg
    26401 ttaaatactc atctcttgct gccctagggc ttgttgctgc aggtgtgcta gcagcttgct
    26461 ccggtggtgc taagaaagaa ggacaagcag ctagcaagaa agaaattatt gttgcaacta
    26521 attcttcacc aaaaccattc aactatgaag aaaatggcga attgactggt tacgagattg
    26581 aagtcgttcg cgctatcttt aaagactctg ataagtatga tgtcaagttt gagaagacag
    26641 agtggtcagg tgtctttgcg ggtcttgacg ctgaccgtta ccaaatggct gttaacaaca
    26701 tcagctacac taaagaacgt gcggagaaat acctttatgc agccccaact gttaaagacc
    26761 ctaacgttct tgtagtcaag aaagacgatt ctagcatcaa atcgcttgat gatgttggtg
    26821 gaaaatcaac agaagttgtt caaggaacaa catctgctaa acagttagaa gactacaaca
    26881 aacaacactc agataatcca actatcctta actatactaa agcaaactta caacaaatca
    26941 tgggccactt gagcgatggt cagtttgact acaagatttc tgataaaatc actgttgaaa
    27001 cagtcatcaa gaaccaaggt ttggacaact tgaaagtcat cgatcttcca agcgaccaac
    27061 aaccttacgt ttacccactt cttgctaaag gtcaagatga gttgaaaaca tttgtagaca
    27121 aacgtatcca agaactctac aaagacggaa ctcttgaaaa attgtctaaa caattcttcg
    27181 gagacactta cctaccggca gaagctgata ttaaataatt tcttgaaatc atccattctg
    27241 agtaagatgg gtgatttttc agttcgctac cgcaatctag tcggaacagt tctctttgga
    27301 gtggtcttga ttgccatctt gcgtttggtc ttttaaatta cccataaaaa aaacttatca
    27361 cagagataga tatcatataa tggcgtaaat gctctttttc tgttaagatt ataaggtatt
    27421 ctattttgga ggaaatgaca tgaaaaaaat cgttaaatac tcatctcttg cagcccttgc
    27481 tcttgttgct gcaggtgtgc ttgcggcttg ctcaggtggt gctaagaaag aaggacaagc
    27541 agctagcaag aaagaaatca tcgttgcaac caatggatca ccaaagccat ttatctatga
    27601 agaaaatggc gaattgactg gttatgagat tgaagtcgtt cgcgctatct ttaaagattc
    27661 tgacaaatat aatgtcaagt ttgaaaagac agaatggtca ggtgtctttg ctggtcttga
    27721 tgctgaccgt tacaatatgg ctgtcaacaa tcttagctac actaaagaac gtgcggagaa
    27781 atacctctat gccgcaccaa ttgcccaaaa tcctaatgtc cttgtagtga agaaagatga
    27841 ctctagtatc aagtctctcg atgatatcgg tggaaaatcg acggaagtcg ttcaagccac
    27901 tacatcagct aagcagttag aagcatacaa cgctgaacac acggacaacc caactatcct
    27961 taactatact aaggcagatt tccaacaaat catggtacgt ttgagcgatg gacaatttga
    28021 ctacaagatt tttgataaaa tcggtgttga aacagtgatc aagaaccaag gtttggacaa
    28081 cttgaaagtt atcgaacttc caagcgacca acaaccgtac gtttacccac ttcttgctca
    28141 gggtcaagat gagttgaaat catttgtaga caaacgcatt aaagaactct acaaagacgg
    28201 aacgcttgaa aaattgtcta aacaattctt cggagacact tatctaccgg cagaagctga
    28261 tattaaataa tttcttgaaa tcatccattc tgagtaagat gggtggtttc tcagttttta
    28321 gggatatagt tttaaactat atatctgact atctaataac aatttgtgaa atcaaacaaa
    28381 ttgtgagata ctagtacggt attattttta aggagaaaga atcatgaaaa tcaaaaaatg
    28441 gcttggccta gcagcccttg ctacagtcgc aggtttggct cttgcagctt gcggaaactc
    28501 agaaaagaaa gcagacaatg caacaacgat caaaatcgca actgttaacc gtagcggttc
    28561 tgaagaaaaa cgttgggaca aaatccaaga attggttaaa aaagacggaa ttaccttgga
    28621 atttacagag ttcacagact actcacaacc aaacaaagca actgctgatg gcgaagtaga
    28681 tttgaacgct ttccaacact ataacttctt gaacaactgg aacaaagaaa acggaaaaga
    28741 ccttgtagcg attgcagata cttacatctc tccaatccgc ctttactcag gtttgaatgg
    28801 aagtgccaac aagtacacta aagtagaaga catcccagca aacggagaaa tcgctgtacc
    28861 gaatgacgct acaaacgaaa gccgtgcgct ttacttgctt caatcagctg gcttgattaa
    28921 attggatgtt tctggaactg ctcttgcaac agttgccaac atcaaagaaa atccaaagaa
    28981 cttgaaaatc actgaattgg acgctagcca aacagctcgt tcattgtcat cagttgacgc
    29041 tgccgttgta aacaatacct tcgttacaga agcaaaattg gactacaaga aagcactttt
    29101 caaagaacaa gctgatgaaa actcaaaaca atggtacaac atcattgttg cgaaaaaaga
    29161 ttgggaaaca tcacctaagg ctgatgctat caagaaagta atcgcagctt accacacaga
    29221 tgacgtgaaa aaagttatcg aagaatcatc agatggtttg gatcaaccag tttggtaata
    29281 agaaacaggg aggtgggaga gaaaattcca cctcttgctt ttgtatagag tatagattgt
    29341 aaagaagact attcgttcat agaaaggtag agagaatatg gtttttccta gcgaacaaga
    29401 acagattgaa aaatttgaaa aggatcatgt agtccaacat tattttgagg ttttgcgtac
    29461 cttgatttct aagaaatcag tctttgccca gcaggttgga ctcaaggaag tcgcaaatta
    29521 tctgggtgag attttcaagc gtgttggagc tgaagtggag attgatgaga gctatacagc
    29581 gccctttgtc atggcacatt tcaagagttc gcgtccagat gccaagacct tgattttcta
    29641 taaccactat gacactgtgc cagcggatgg ggatcaggtc tggacagagg atccttttac
    29701 gctttcggtc cgcaatggct tcatgtatgg gcgtggggtt gatgacgaca agggtcatat
    29761 cacagctcgc ttgagtgctt tgagaaaata tatgcagcac catgatgatt tacctgtcaa
    29821 tatcagcttt atcatggagg gagcggagga atcggcttca acagacctag ataagtattt
    29881 ggaaaagcat gcagacaaac tccgtggggc ggatttgttg gtctgggaac aagggaccaa
    29941 aaatgccttg gaacagctgg aaatttctgg tggcaataag gggattgtga cctttgatgc
    30001 caaggtaaaa agcgctgatg tggatatcca ctcgagttat ggtggtgttg tggaatcagc
    30061 tccttggtat ctcctccaag ccttacagtc tcttcgtgct gcggatggcc gtatcttggt
    30121 tgaaggcttg tacgaagaag tacaagagcc caatgaacga gaaatggcct tgctagaaac
    30181 ttatggtcaa cgaaacccag aggaagttag tcggatttat ggattggagt tgcctctctt
    30241 acaggaggag cggatggcct ttctaaaacg tttctttttc gagccagcgc ttaatatcga
    30301 aggaatccag tctggttatc aaggtcaggg tgttaagact attttacctg cagaagccag
    30361 tgccaagcta gaggttcgtc tggttccggg cctagaaccg catgatgttc tggaaaaaat
    30421 tcggaaacaa ctagacaaaa atggctttga taaggtagaa ttatactata ccttgggaga
    30481 gatgagctat cgaagcgata tgagcgcacc agccattctc aatgtgatcg agttggccaa
    30541 gaaattctat ccacagggcg tttcagtctt gccgacgaca gcggggacag gacctatgca
    30601 tacggtcttt gatgccctag aggtaccaat ggttgcattt ggtctaggaa atgccaatag
    30661 ccgagaccac ggtggagatg aaaatgtgcg aatcgctgat tattacaccc atatcgaatt
    30721 agtagaggag ctgattagaa gctatgagta gagatattat caagttagat cagatcgatg
    30781 tgacttttca ccaaaagaag agaaccatca cagcggttaa ggatgtgacc attcacatcc
    30841 aagaagggga tatctacgga atcgttggat attctggagc agggaaatca acccttgtac
    30901 gggtgattaa cctcttgcaa aaaccatctg cagggaaaat taccattgac gacgatgtga
    30961 tttttgatgg taaggtcacc ttgacggcag agcagttgcg tcgtaaacgt caagatatcg
    31021 ggatgatttt ccagcatttt aacctgatga gccaaaagac agcagaggag aatgtagcct
    31081 ttgcccttaa acactctgga ctcagcaagg aagaaaagaa ggctaaagta gctaagttgt
    31141 tggacttggt tggtttggca gatcgtgctg aaaactaccc ttcacaacta tctggagggc
    31201 aaaaacagcg tgtggcaatt gcgcgtgcct tggccaatga tccaaaaatc ttgatttcag
    31261 acgagtcaac ttctgccctt gatccgaaga caaccaagca gattttggcc ttgttgcaag
    31321 atttgaacca aaaattaggc ttgactgttg tcttgattac gcatgaaatg cagattgtca
    31381 aagacattgc caaccgtgtt gcagttatgc aggatgggca tttgattgaa gagggtagtg
    31441 tgcttgaaat cttctcaaat cctaaacaac ctttgactca agactttatc tcaacagcta
    31501 caggtattga cgaagccatg gtcaaaatcg agaagcaaga aatcgtggaa cacttgtctg
    31561 aaaacagtct cttggtgcaa ctcaagtacg ctggagcttc aacagacgag ccacttttga
    31621 atgaattgta caagcattac caagtaatgg ctaatattct ctatgggaat atcgaaattc
    31681 tcgatggtac tcctgttgga gaattggtgg tggtcttgtc aggtgaaaaa gcagcgttgg
    31741 caggtgccca agaagccatt cgtcaagcag gtgtacaact aaaagtattg aagggaggac
    31801 agtaagatgg aatcattgat tcaaacctat ttaccaaatg tctataaaat gggttgggct
    31861 ggtcaggcag gctggggaac ggctatctac ttaactcttt atatgacagt tctttccttc
    31921 attatcggag gcttcttggg gctagtggca ggtctctttc tcgtcttgac agcgccaggt
    31981 ggtgtcttgg agaataaagt cgtattctgg attttagaca aaattacctc aatttttcgt
    32041 gcagttccct ttatcatcct cttggcaatc ttgtcaccac tttctcactt gattgttaaa
    32101 acaagtatcg ggccaaatgc agcccttgtc ccactttctt ttgcagtctt tgccttcttt
    32161 gcccgtcagg tgcaggttgt cttggctgaa ttggatggcg gtgtcattga ggcggctcaa
    32221 gcgagcggag cgactttctg ggacatcgtg ggtgtttacc tatcagaagg tcttccagat
    32281 ttgatccgtg tgacgactgt gaccttgatt tcccttgttg gggaaacagc tatggccggt
    32341 gcggttggag ctggtggtat cggtaacgta gccatcgctt atggatttaa ccgctacaat
    32401 cacgatgtga ccatcttggc aaccatcatt atcattttga ttatctttgc aatccaattc
    32461 ttgggagatt tcttgactaa gaaattgagc cataaataaa aaagagccgc gtggctcttt
    32521 ttaactgatc agattttctg tgcaaatttt ttactcaagg cttgtccaat caaggcaccc
    32581 actagggctc cgatgacaat acttgcgata aatagaagga cagttccagg gtttggagcg
    32641 accatgatgc ggtcgatata ttcttgggat tttcctcttg ccagaagagt agccatatag
    32701 gctttgggcg caatccacat aagcaagatt ggtcctgttg tactaaaggc gaaaataatg
    32761 aaagaaagga agttctttgt tttgtccttg tattttccta aatgagctac tccatctgct
    32821 aggaggccac agataattcc aggaaggaag gaaccagcac cgtgtttagt tcccaagaaa
    32881 aagagggcaa tgacaaggcc gatagtggta atggctccaa agcgcggaac ttttgcgact
    32941 aggatcatat agacgctacc gccgacaagg gcagtaaagg caggcgcata aaacatgttt
    33001 ccagtttggt caaagagatt gcccaaaagg acaccaatcc ccatgcagag gaagtaaaga
    33061 actgcaaaaa gcagtgtagt taagatagat ttcttcatga attgtctcct gataattttt
    33121 tcacaattct cattttacca tggtttggtg ggattgtaaa tggatactag aagtttttga
    33181 aaacgcttga aatatgatat aatggtttca tagttatttt taggggaata tcatgggtag
    33241 atttttagac tttgtcttta atcgtttctt tttagggatg attgcgacag ccttcttttg
    33301 gctattaact ttagcaggag ggattatcct tggtctagcg ccggctagtg ccaccttgat
    33361 gagcttatat gcagaacatg gttatagctt tcgggaatac agtttgaagg aggcttggtc
    33421 tctttacaag caaaattttg tctcaagcaa cctgattttc tatagctttt taggtgtggg
    33481 tctagttttg acctatggtt tgtatctctt ggtgcaattg cctcatcaga ctattgttca
    33541 tttgattgcg acccttttga atgtcctagt agttgccctg atctttttgg cttatacagt
    33601 atctttaaaa ttacaagttt attttgcctt gtcctatcga aatagtctca aattatcctt
    33661 gattggcatc tttatgagtc tagcagctgt ggccaaggtt ctccttggga ctgtgctact
    33721 tgtagcaatt ggttactata tgcctgccct gctatttttt gtaggaattg ggatgtggca
    33781 tttctttatc agtgatatgt tggaacctgt ctatgaaatc atccatgaaa aattggcgac
    33841 aaaatagaat gaagcagttt tggctacata cgcttctaag aacctatagt tcagtgatga
    33901 tcattatcat tgcgagtttt gcaatcttac tctcttacgc tgactgggat tcacgtgaaa
    33961 aggaagccca gagagtagcc cagcgtgtaa ctgctagaac agtgagtgaa attgaatatt
    34021 accatagaga gtcaacccag atagctcagg ctttagttga aaatcaagct cgtattgagg
    34081 gaatctataa atactttagc cttagcatgc cagactattt ttactggcaa ttagagcgga
    34141 aagcttcgcc ttatatatca gtctctctgt atgaaaatgt tgatgacctc tatgttcgaa
    34201 atgattttgt aactggggtg gccattgctt ttcaagatta caaggaagtc tatgtttcta
    34261 ctaaagacaa acgtagtgga gaaaaaatca gggctgagga tttcaaacca gcaggaaata
    34321 gttttgccat tccggtgtca gatccagtgt cagatcaaga tttaggagtg atttacatct
    34381 ccttggatcc tgctgtttta taccatgcca ttgataatac tagaggtcat actccgatgg
    34441 cagtaacagt gacctcacct tttgatacgg agatttttca tattggtgag acagttgata
    34501 gggagagtga aaattggcta gttggcttaa cttctcatgg atatcaggtt caggtggcag
    34561 ttcctaaaaa ctttgtttta caaggaacag tgactagctc tgctttgatt gtgggtttga
    34621 gccttctctt tattgtcatt ctttatctga ctttgaggca gacttttgct aattaccaaa
    34681 agcaggtagt ggatttagtg gaatccattc aagtcattgc tcaaggcgaa gaggggcgtc
    34741 ggattgacat ttccgaaaaa gatcaggaat tactcctaat cgcggagacg accaatgata
    34801 tgttggatcg attggaaaag aatatccatg atatttacca gttagagctt agtcaaaaag
    34861 atgccaatat gcgagccttg caggcgcaaa tcaatcctca ttttatgtat aatacgctgg
    34921 agttcttgcg catgtatgca gttatgcaga gtcaagatga gttggcagat atcatttatg
    34981 aattcagtag tctcttgcgt aacaatattt ccgacgaaag agagaccctc ctcaaacagg
    35041 aattagaatt ttgccgtaaa tacagctatc tctgcatggt tcgctatccc aagtccattg
    35101 cctatggttt caagatagat ccagagttag agaatatgaa gattcccaag tttaccttgc
    35161 aaccgctggt agaaaactat ttcgcgcatg gtgttgacca caggcggaca gataatgtga
    35221 ttagcatcaa ggctcttaaa caggatggtt ttgtggaaat tttggtggtc gataatggta
    35281 gaggaatgtc ggctgaaaag ttggcaaata tccgagaaaa attaagtcag aaatattttg
    35341 aacaccgagc cagctacagt gatcaaaggc agtctatcgg gattgtcaat gtacacgagc
    35401 gttttgtgct ctattttgga gaccgctatg ccattacgat agagtctgca gagcaagccg
    35461 gtgttcagta tcgtattaca attcaagatg agtagaaagg gagaaaatgt ataaagtatt
    35521 attagtagat gatgagtaca tggtgacaga aggtctgaag cgtttgattc cctttgataa
    35581 gtgggatatg gaggtcgtcg caacagccag tcatgccgat gaagctctag aatatgttca
    35641 ggaaaatcct gtcgatgtca tcatttccga tgtcaatatg ccagacaaaa cagggcttga
    35701 tatgattcgg gagatgaaag agatcttacc agatgctgcc tatatcctgc tctcaggtta
    35761 tcaggagttt gattatgtaa aaagagcaat gaaccttagt gtggtggact atttggtcaa
    35821 gcctgttgat aaggtagagc tgggaaatct gctggagaag attgcaggtc agctcggcga
    35881 gagagggaag aaaagtcaga ctcttagtca agaattagac gaggctggat ttgttagtta
    35941 tttaggggat aaggagaatt ggtggatagg tctatccaag gaaaaacaag gttccttcac
    36001 cattccctac tatgtcttgg gccaagactg gcagattttc atttctgacc aacccctaga
    36061 tggtttagtc gttacacctt ttgaagctcc ttatcaagaa cactttgaac gctggaagct
    36121 gaatgctgag aaaaccctct tttacggttc tgtaaatttg cagcagtctg agagtctctt
    36181 tgcctattac gaaccgattt atagggttat cattcaggga aatctcaatc aaatcgtaga
    36241 agagttaaat ctcttggaga aggtagttct tgaaaatacg ccgcgtgttc cgattactaa
    36301 acagcttttt attcagtttg tcatggatgt tttccattta tttgaacatc tcaaagctga
    36361 tgacctgaca gacatcgtca aaaccattca tgctattcaa tccttcgatg aattggttcc
    36421 ttatatcaag gaaactctga gcagcttttt cggtcaatac cgtatgaatg aaaatgtggt
    36481 cagtgtgctg gaagtcattg gttgtgatta ccaaaaagag ctttccctca aggatatcag
    36541 taaggctctc tttatcaatc ctgtctatct agggcagttg attaagcgtg aaaccgattc
    36601 gacttttgca gagttactaa acaaacaacg tattaaggct gcccaacaac ttttgctttc
    36661 aactagtgac agcatcgaag atatttgtta tgctgttggt tacagtaacc ttggatattt
    36721 ctataaagtt ttccgaaaat tgtgcggaaa atcgccaaaa gcctaccgaa aacaggtaga
    36781 aactatacta taagatttgt attcctttac aaaatgtgct ataatataaa tattattatt
    36841 aggaggttct atcatgaaaa agaaaccaat ttatctatgg gtcttgctaa tcttgtctgc
    36901 ccttatttca gttccgtctc tgtttggaat tgtgagtccc ttgcctagta aagaagccct
    36961 tcgtactgct cagaaacaag ttgcaggggt caatgctcag caattagaag accagcttaa
    37021 ttatacctat agagtagcag aagcatctca ttctattttt aatgttgctt tgattgtgct
    37081 atctactatt ttagtggtcg tagcgattgt cttccttgtt cgtaaaaatt tgcaatatgc
    37141 aaactatact tacgttggct atgttttgtt agctattatt ggttcgattt atggctatgt
    37201 tggtttacaa gacgctgtgc agttggttca agatgagagc atgcgtttga cagtgagtat
    37261 tggttcaaaa gctgttagca ttttctatat cgtcatcaat gttctcttcc tagcccttgt
    37321 cttctataag atgtggcgac aacagaaagc ctcggcagaa gaagaggaaa cagaagagct
    37381 tgcctaagta ttgacaaaaa agaactatca agatacaatc ttggtagttg ttttttgatt
    37441 atagataaaa tcatggaggt acatatgaag ataatttctt tagtttatat tagtttgagt
    37501 ggcaacactg aaagttttgt gacgcgcttg aaagactatc tcttgtccca gtacaaaggg
    37561 attgaggttc aaaagattca tatcaaggat ttagtcaagg aaggcaaaaa tttctatgaa
    37621 atggaccatc cctatgtcgc ttttttgccg acctatctcg aaggtgggaa tggcgtggat
    37681 aacggagatg ttgagatttt gacgacaccg gtgggagatt ttattgccta tggtaacaat
    37741 gctagtaagt gttttggtgt ggttgggtca ggaaatcgta actttaataa ccaatactgc
    37801 ctaacagcca agcaatacag tcaacgcttt ggctttcctg tattggctga ctttgaaatg
    37861 cgaggcatgc tggaagatat caaacatgtc gcagctatta tcgcagattt gtatgagttg
    37921 gaaaaggaga attaatactc aatgaaaatc aaagagcaaa ctaggaagct agtcgcaggt
    37981 tgctcaaagc acagctttga ggttgcagat agagctgacg tggtttgaag agattctcga
    38041 atagtataaa attcttataa gaggtggttg aagttgttca actgtttttt gagtataaac
    38101 agtctttaag aacgtaaaaa agcataagtt caggtcttca aaaccttgtt cttatgcttt
    38161 ttatcataga aatatttgct gaattttctc ttgaaatgaa accttatctt catctcgcgc
    38221 ttgtccctta ccctgctttt ccgattgcga gtgcgtagat aaagaagatg gcgaagccaa
    38281 agaggaaaat caatcccgca atggcaaggt gtttgagcca agaaggaaga tttttgtttt
    38341 cacgcgttac caaggcgtaa ttcaaaagag caaagaaagg tgttgtcagg aaagagccaa
    38401 tcatggcaaa gcggagcatg gttgaaacct gaccagagaa gaacttgata atgacgatac
    38461 cgatgatagc agtgatggtc atccagatgt tcaaagattt acgattgtcc tctttttgac
    38521 tgattagcag tcggagagat tcctgattaa cgcgagaata gccatcgata acagttataa
    38581 ctgttccaaa gatacagagg aaggcaataa aggtaatcaa gtaacgggac cattcgccaa
    38641 gaacagaggc atacatgccc acgaattgag agatgtattt ggctgaagca gcttcaaccg
    38701 cctgccctgt aggatactga atcagtgctc ccagtgccac aaagaagacg gctaggatag
    38761 ctgttccaat ataaccagtg ttaaagtcaa acagagcgtc ttctgtgtta aagttgacgg
    38821 tctttctctt ttcagctgac caaagtgaat tgatggctga aatttcaata ggagccggca
    38881 tccatcctag gagggagacg atgaagggca gagctgccat ttgccaaggt gtcttctcga
    38941 caaaatcaga actgtattct ggatgcttga ccgccgcaat gataactgcc agaacagtcg
    39001 caatggttaa ggcagacata atccatttga ccatgccgtc taaaagtttg tagcctccaa
    39061 agagtagcat agcccaaatg attgcaacga gaatgaggga ccactgagta atgctaagtc
    39121 caatcattgg gaaggcactg gcgatgatag ctgagcacag aatggcaaca ccagccgtgt
    39181 tgaccatagc cgaaaagaca ttgaggataa agaaaatcca gagatagagt tttccttttt
    39241 cggcataacc ttcaaccaaa gtctttccag tatcagctgt gtattcagca ccaaaacgga
    39301 aaaatggata tttaaagaca ttggctaaga tgaccaagag aagtagagac caaccgtaag
    39361 aaccgccagc ttgagttgag gatacaatgt gggaacctcc aacagcggca gttgccatta
    39421 ggattccagg tcccattgct tttagcttgc ttgcccaggt tgattgattt aaggaaatag
    39481 cttgcgacat gataaatact cctttttgaa ttttcagaat aatctgtata tgtaatctat
    39541 attctacaaa aaattttgga aaaatgcaag aatattttga aaaaagatag aaaatttaca
    39601 aagaaaat
//
LOCUS       DAVBCB010000013        37669 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVBCB010000013 DAVBCB010000000
VERSION     DAVBCB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37669
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.013"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            108..896
                     /gene="codY"
                     /locus_tag="VQU28_000660"
     CDS             108..896
                     /gene="codY"
                     /locus_tag="VQU28_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HET2476048.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            896..1471
                     /locus_tag="VQU28_000661"
     CDS             896..1471
                     /locus_tag="VQU28_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HET2476049.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDFFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            1553..1638
                     /locus_tag="VQU28_000662"
     tRNA            1553..1638
                     /locus_tag="VQU28_000662"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1587..1589,aa:Leu,seq:aag)
     gene            1921..3051
                     /gene="ugpC"
                     /locus_tag="VQU28_000663"
     CDS             1921..3051
                     /gene="ugpC"
                     /locus_tag="VQU28_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HET2476050.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(3198..3344)
                     /locus_tag="VQU28_000664"
     CDS             complement(3198..3344)
                     /locus_tag="VQU28_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476051.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            3552..4310
                     /locus_tag="VQU28_000665"
     CDS             3552..4310
                     /locus_tag="VQU28_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2476052.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGLSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            4397..4909
                     /locus_tag="VQU28_000666"
     CDS             4397..4909
                     /locus_tag="VQU28_000666"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HET2476053.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            5091..6035
                     /locus_tag="VQU28_000667"
     CDS             5091..6035
                     /locus_tag="VQU28_000667"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HET2476054.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            6044..6721
                     /locus_tag="VQU28_000668"
     CDS             6044..6721
                     /locus_tag="VQU28_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HET2476055.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVRSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            6819..7577
                     /locus_tag="VQU28_000669"
     CDS             6819..7577
                     /locus_tag="VQU28_000669"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HET2476056.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            7615..9120
                     /gene="lytC"
                     /locus_tag="VQU28_000670"
     CDS             7615..9120
                     /gene="lytC"
                     /locus_tag="VQU28_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HET2476057.1"
                     /translation="MSVTFFIGEERLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSTEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDLN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAESDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTGTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPHYSGKKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            9616..10134
                     /locus_tag="VQU28_000671"
     CDS             9616..10134
                     /locus_tag="VQU28_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HET2476058.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            10264..10770
                     /locus_tag="VQU28_000672"
     CDS             10264..10770
                     /locus_tag="VQU28_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HET2476059.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            10770..10940
                     /locus_tag="VQU28_000673"
     CDS             10770..10940
                     /locus_tag="VQU28_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476060.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            10972..12204
                     /gene="clpX"
                     /locus_tag="VQU28_000674"
     CDS             10972..12204
                     /gene="clpX"
                     /locus_tag="VQU28_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HET2476061.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            12213..12800
                     /locus_tag="VQU28_000675"
     CDS             12213..12800
                     /locus_tag="VQU28_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HET2476062.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            12811..13191
                     /locus_tag="VQU28_000676"
     CDS             12811..13191
                     /locus_tag="VQU28_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HET2476063.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            13243..14133
                     /gene="rapZ"
                     /locus_tag="VQU28_000677"
     CDS             13243..14133
                     /gene="rapZ"
                     /locus_tag="VQU28_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HET2476064.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLAQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            14130..15107
                     /locus_tag="VQU28_000678"
     CDS             14130..15107
                     /locus_tag="VQU28_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HET2476065.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            15104..16015
                     /gene="whiA"
                     /locus_tag="VQU28_000679"
     CDS             15104..16015
                     /gene="whiA"
                     /locus_tag="VQU28_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HET2476066.1"
                     /translation="MSFTVAVKEEILGQHHLSRHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLVNGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(16049..17017)
                     /locus_tag="VQU28_000680"
     CDS             complement(16049..17017)
                     /locus_tag="VQU28_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2476067.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            17153..18010
                     /locus_tag="VQU28_000681"
     CDS             17153..18010
                     /locus_tag="VQU28_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HET2476068.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            17997..18776
                     /locus_tag="VQU28_000682"
     CDS             17997..18776
                     /locus_tag="VQU28_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HET2476069.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            18800..20152
                     /locus_tag="VQU28_000683"
     CDS             18800..20152
                     /locus_tag="VQU28_000683"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HET2476070.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAIQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            20226..20600
                     /locus_tag="VQU28_000684"
     CDS             20226..20600
                     /locus_tag="VQU28_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HET2476071.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            20602..21450
                     /locus_tag="VQU28_000685"
     CDS             20602..21450
                     /locus_tag="VQU28_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2476072.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            21727..22494
                     /locus_tag="VQU28_000686"
     CDS             21727..22494
                     /locus_tag="VQU28_000686"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HET2476073.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDPDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDDMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            22506..23690
                     /locus_tag="VQU28_000687"
     CDS             22506..23690
                     /locus_tag="VQU28_000687"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HET2476074.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            23687..25558
                     /locus_tag="VQU28_000688"
     CDS             23687..25558
                     /locus_tag="VQU28_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2476075.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDSKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(25701..26885)
                     /gene="mntE"
                     /locus_tag="VQU28_000689"
     CDS             complement(25701..26885)
                     /gene="mntE"
                     /locus_tag="VQU28_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HET2476076.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPISEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            27189..29885
                     /locus_tag="VQU28_000690"
     CDS             27189..29885
                     /locus_tag="VQU28_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HET2476077.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            29898..30689
                     /gene="yghU"
                     /locus_tag="VQU28_000691"
     CDS             29898..30689
                     /gene="yghU"
                     /locus_tag="VQU28_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HET2476078.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            30805..31215
                     /locus_tag="VQU28_000692"
     CDS             30805..31215
                     /locus_tag="VQU28_000692"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2476079.1"
                     /translation="MEKKIVKDILFLSQVSHPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGMQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRL
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            31227..32822
                     /locus_tag="VQU28_000693"
     CDS             31227..32822
                     /locus_tag="VQU28_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476080.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            32989..33153
                     /locus_tag="VQU28_000694"
     CDS             32989..33153
                     /locus_tag="VQU28_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HET2476081.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            33232..33960
                     /gene="yaaA"
                     /locus_tag="VQU28_000695"
     CDS             33232..33960
                     /gene="yaaA"
                     /locus_tag="VQU28_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HET2476082.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(34011..35495)
                     /locus_tag="VQU28_000696"
     CDS             complement(34011..35495)
                     /locus_tag="VQU28_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2476083.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILERLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEGYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYISGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
     gene            35711..>37669
                     /locus_tag="VQU28_000697"
     CDS             35711..>37669
                     /locus_tag="VQU28_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HET2476084.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAVSVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTGEV
                     TKPAEKVEEAKDKKEEVTHQDAIDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADASEAELSAAESSIQSSIIGLELRSNSNKGTVSETPVAKKANIVEAKEETN
                     PAATTDRSAVDSAVLPTSTAAKVETTSAPASINEILKPGLSLSDARQNPAIRKEDVDR
                     GYSGFRAAPAPTNQPVTNLGNNTVFTDISRGSHTFRGNGNSRRGNPIHFDVTTTRVRN
                     RVDFSIRYSGPGEFVNNNFILDKGNGFGEPSRATVSTPKLRDQVKNISKGGNFVSHSG
                     YSLEAAVETSTNQTIKFSLPIANPNGDLSVRLRPVTFNVGQGESGSATSNDPYSNSNY
                     YFREKPLLLDANPNGGTNNKTVSEDIDFQTVYLPTSKLPEGQTRLVREGEKGQRQIIY
                     KVHQLGNETILGLPISNSVIKEVKPRIMQIGVAKDLIDTVKPRVDQNKVGDTNNLTFY
                     LDNDGNGVYTEGVDELVQKIAIKDGAKGEKGDRGERGLTGAQGAKGEKGDRGERGLTG
                     AQGAKGEKGDRGERGLTGAQGAKGEKGDR"
BASE COUNT        11318 a         6899 c         8567 g        10885 t
ORIGIN      
        1 tttaattaaa ttgtaagtca atgcaagtca ataaggaatt ttcagataat tattgaaaaa
       61 gagacgtttt tatgttataa tgatagcgat taaattcgag gtgaaaaatg gcacatttat
      121 tagaaaaaac tagaaaaatt acatcaattt tgaaacgctc agaggagcag ttgcaggatg
      181 agctccctta caacgctatt acgcgtcagt tagcggatat tattcattgc aatgcctgca
      241 ttatcaatag taagggacgt ctgcttggct attttatgcg ttataaaaca aatacagatc
      301 gcgtagagca attcttccaa actaagattt tcccagatga ctacgttcaa ggggctaata
      361 tgatttacga aacagaagca aacttgcctg ttgagcatga tatgagtatt ttccctgttg
      421 agagtagaga tgattttcca gatggcttga cgactattgc accgattcat gtatcgggga
      481 ttcgccttgg ttctttgatt atttggcgta atgataaaaa attcgaagat gaggacttgg
      541 ttcttgttga gattgccagt accgttgttg ggattcagct tcttaacttc caacgtgaag
      601 aagatgagaa aaatattcgt cgtcgtactg ctgtcaccat ggcggtcaat accctttctt
      661 actccgaact ccgtgctgtt tcagcaattt taggggaatt aaatggaaat gaagggaagt
      721 tgactgcgtc agtgattgca gatcgtatcg gaatcactcg ctctgtgatt gtcaatgctc
      781 ttcgtaaact tgagtctgcg gggattattg aaagtcgctc acttggaatg aagggaacct
      841 atcttaaggt cttgatttca gatatttttg aagaagtgaa gaaaagagat tactaatgac
      901 aaaggcttta atttcgattg attatacaga agattttgtt gctgatagtg ggaagctgac
      961 agcaggtgct ccagctcagg cgatttcgga tgccatcagc aaggtaactc gattagcttt
     1021 tgaacgagga gattatatct tctttactat tgatgctcat gaagaaaacg attttttcca
     1081 tccggaaagt aagttatttc ctcctcataa tctgattggg acgagtggac ggaatttata
     1141 tggagatttg gggatctttt atcaagagca tggttcagac agtcgtgtct tttggatgga
     1201 taaacgccat tactcagctt tttcagggac tgacctagat attcgtttga gagagcgtag
     1261 agtgtctacc gttatcttaa caggtgtctt gacggatatc tgtgtcctac atacagctat
     1321 agatgcctat aatctaggat atgacatcga gattgttaaa ccagctgttg cttccatctg
     1381 gcctgaaaat catcaatttg ccctaggtca tttcaaaaat acactcggag ctaagttagt
     1441 agatgaaaat ctaaatgaac tttctgagta acctggttgg tgaaaatgaa aaaatttgaa
     1501 aaaaagtgtt gacaagcatc ccaaaagttg atatactagt aaagtaatcg atgcggggat
     1561 ggcggaattg gcagacgcgc aggactaagg atcctgtgac cgctttaggt cgtgagggtt
     1621 caagtccctc tctccgcata ggaaaagagt tagctaaagc tagctctttt gtatttctct
     1681 aaaaaattag aatccagtca aatgtagtgg agaatcagcg aaaatatggg aaaactgttc
     1741 cttgtttctg cttttgtcat agaagaataa taaagattat cttcttcaaa ttctccgata
     1801 ttctctaaag ttttgtgcaa gttgcacaga acttttttat ttttttggtc atcttgccat
     1861 agaaatataa agcgttttca tctataatat aattatcaaa agacaaaagg agttcacctc
     1921 atggtagaat tgaatcttaa aaatatttac aaaaaatatc caaacagcga acactattca
     1981 gttgaagatt tcaacttgaa catcaaagat aaagaattta tcgttttcgt aggaccttca
     2041 ggatgtggta aatcaactac actccgtatg attgctggtc ttgaagacat tacagaaggt
     2101 actgcatcta tcgatggcgt ggttgtcaac gacgtagctc caaaagaccg tgatatcgcc
     2161 atggtattcc aaaactacgc tctttaccca cacatgactg tttatgacaa catggctttc
     2221 ggtttgaaat tgcgtaaata cagcaaagaa gacattaaca aacgtgttca agaagcagct
     2281 gaaatacttg gattgaaaga attcttggaa cgtaaaccag ctgacctttc aggtggtcaa
     2341 cgtcaacgtg ttgccatggg gcgtgcgatt gtccgtgatg cgaaagtatt cttgatggac
     2401 gaacctttgt caaacttgga tgccaaactt cgtgtatcaa tgcgtgctga aatcgctaaa
     2461 attcaccgtc gtatcggagc tacaactatc tatgtaactc acgaccaaac agaagcgatg
     2521 acacttgcag accgtatcgt tattatgtca gctactaaga accctgctgg tacaggtact
     2581 atcggacgtg tagaacaaat cggtactcct caagaagttt acaaaaatcc agttaacaaa
     2641 ttcgttgcag gattcatcgg aagcccagct atgaacttca tcaacgtgaa attggttggt
     2701 agcgaaattg tttctgacgg tttccgtttg aaagtgccag aaggagcatt gaaagttctt
     2761 cgtgaaaaag gctacgaagg aaaagaattg atctttggta tccgtccaga agacgtgaat
     2821 gcagaacctg ctttccttga aacattccca gactgtgttg taaaagcgac tatctctgta
     2881 tcagaactgc ttggttcaga atctcacctt tactgccaag ttggtaaaga cgagtttgtt
     2941 gcaaaagttg atgctcgtga ctacttgcaa acaggtgcaa cagttgagct tggatttgac
     3001 ttgaacaaag cacacttctt cgatgtagaa actgaaaaaa caatctacta aaataaataa
     3061 aattcaaagc actacaagaa aagatatctc tttatcaatt gtagtggaga gatatcagtt
     3121 aatctaggga gagaaacaaa atgcttctct cctttttgct agggaagtca tattatgcat
     3181 ctatattgtg atgctcttta atactcttcg aaaatctctt caaaccacgt caacgtcgcc
     3241 ttgccgtacg tatgattact gatttcgtca gttttatctg caacctcaaa gatgtacttt
     3301 gagcagctta cggctagttt cctagtttgc tctttgattt ccattgagta ttatttgtgg
     3361 gtaccatcta caagtgaagc tatatgcgta aactacgtga gcaattgaat tcgaactaga
     3421 gaggtaataa taaatttatg ctatagttat ggtgacttgt atgcttttga ttctagttta
     3481 tcaaataata gattagaatt gtcagataat atcattttgt gttataatga agaaaaaaca
     3541 gaggtgttca aatgtcagaa gcaggtcata agtttttagc aaaattgggg aaaaaacgct
     3601 tacgtccagg tggaaagcgt gctacagatt ggttaattgc agaaggagga ttgtcaaaag
     3661 aaaagagaat actagaggtt gcgtgtaata ggggaactac agcaattgag ttggcacagc
     3721 gttttggttg caagataact gctgttgata tggatgctca agctttagaa gtggctaaaa
     3781 aatctgctgg aacggcaggt gttgctcatt taatcagttt tgaaagagca aatgcaatga
     3841 aacttcctta tcaagatgct agttttgata ttgttataaa tgaagctatg ctgactatgc
     3901 aagccgatca agctaagaaa aaatgtgtaa tggaatatct aagggtatta aaacctggag
     3961 gtcttctttt gacacatgat gtgcttctta aggaagctaa agagtctatc agacaggaat
     4021 tatcacaagc aattcatgta aatgtaggtc ctttaactca agatggttgg gaacaggtga
     4081 tgatagaatc aggttattgt gatgtgaaag tattgactgg tgaaatgaca ttaatgaaat
     4141 tatcgggtat gatttatgac gaaggttggc taggaacttt gaaaatttgt gtaaatgctt
     4201 gtaaaaagga gaatagaaag cagtttttaa ctatgtataa aatgtttgct aagaataaac
     4261 agaaattggg ctttattgcg atggctagtt ataaatcgtc aaaacgttag ataattattg
     4321 aagttaactt ttcctttttt ctttcttaaa aaatatgcta taatagagag tgaaaaactt
     4381 tgaaagaaag aaaaagatga atttaaaaga ttacattgca acaattgaaa attatccaaa
     4441 ggaaggcatt accttccgtg atattagtcc tttgatggct gatggaaatg cttatagcta
     4501 cgctgttcgt gaaatcgttc agtatgctac tgacaagaaa gtcgacatga tcgtgggacc
     4561 tgaagctcgt ggatttatcg tgggttgtcc agttgccttt gagttgggaa ttggttttgc
     4621 gcctgttcgt aagccaggta aattgccacg cgaagttatt tctgctgact atgaaaaaga
     4681 gtacggtgtc gataccttga ctatgcacgc ggatgccatt aagccaggtc aacgtgttct
     4741 tattgtagat gaccttttgg cgacaggtgg aactgttaag gcaactatcg agatgattga
     4801 aaaacttggt ggtgttatgg caggttgtgc cttccttgtt gaattggatg aattgaacgg
     4861 ccgtgaaaaa atcggtgact acgactacaa agttcttatg cattattaat gaaaacagtc
     4921 cctagggctg ttttctctac actaggatat aaaaatagac tataactagt tagagaaaaa
     4981 ctataattga aaactatatc ttcttgcagt ataataaaag gactaagtgt ttgagatttg
     5041 tcttcaaaca catgcaatta ttcctgagag agtacagtta ggagagggtt atgccgattc
     5101 gaattgataa aaaattgcca gctgttgaga ttttacggac agagaatatc tttgtcatgg
     5161 atgatcaacg tgctgcccac caagatatcc gtcctttgaa gattttaatt ttaaatctca
     5221 tgccacagaa aatggtcaca gagacccagt tgttgcgtca cttggctaat acacccctac
     5281 aactggatat tgattttctc tatatggaga gccaccgttc taaaacaact cgttcagagc
     5341 acatggagac cttctataaa acttttcctg aagtcaagga tgagtatttt gatgggatga
     5401 tcatcacggg tgctccagtt gagcatttac catttgagga agtggactat tgggaggaat
     5461 ttaggcagat gcttgagtgg tctaagacac atgtctattc gacccttcat atctgttggg
     5521 gggctcaggc tgggctttat ctgcgctatg gtgtagaaaa ataccagatg gacagtaagc
     5581 tatcaggtat ttatcctcag gacaccctaa aagagggtca ccttctattt agaggctttg
     5641 atgatagcta tgtatcccct cattcacggc acacggagat ttctaaggaa gaggtcttaa
     5701 acaagaccaa tctcgagatt ttatcagaag gacctcaggt tggggtttct attttggcca
     5761 gtcgtgattt acgagaaatt tatagttttg gtcatttgga gtatgaccgt gataccttgg
     5821 caaaagagta ttttcgagat cgtgatgcag gtttcgaccc acatattcca gaaaattact
     5881 ttaaagatga tgatgttaat caggtacctt gtctttgttg gtcttcatct gcagccctct
     5941 ttttcagtaa ttgggtaaac catgcggtct atcaggagac gccttttgat tggagaaaga
     6001 tagaagatga tgcatctgca tatgggtatt tataagagga attatgacat atttagacgc
     6061 ttttaaatca ggtaccttgg ttttaccgag tgccctgctc ttgcatttta aggaactctt
     6121 tccttctagc gacgattttc tggtttggca atttttctat ttgcaaaata cgacaggctt
     6181 agaagaaatg tcgccaagcc agattgctga aaggattggc aaggaaattt cggatgtcaa
     6241 ccagtccatt tctaatctga cggaaagggg actgctccag tatcgtacta tcgaattaaa
     6301 tggcgaaatt gaattgctct ttgatgctag tttggccttg gaacgtttgg atgacctgtt
     6361 tggagcagtt cgttcaagtt cagaccagct aacacctcaa aaccagctca aggatttggt
     6421 ggaaaccttc cagcaggagt tgggacgatt gttgacgcct tttgagattg aggatttgac
     6481 caagacacta aaggaagatg gaaccagtgc tgacttgatt aaggaggctc ttcgtgaagc
     6541 tgttttgaat ggaaaaccaa actggaagta cattcaggcg attttgagaa actggcgcca
     6601 tgaaggaatc aagagtgtgg ctcaaattga ggccaagaga gcagaaagag aagcaagcaa
     6661 tcctcagttg acacaggtat ctgcagattt cagaaatgcc atggatctct ggaaggatta
     6721 atccatgcaa gtaggcttga aatccgagta agatttgcaa gctgtgtata attgtgatag
     6781 aataaataga aaataaattg aaaaaagagg tatgtgaaat gtcacgtaaa ccatttatcg
     6841 ctggtaactg gaaaatgaac aaaaatccag aagaagctaa agcattcgtt gaagcagttg
     6901 catcaaaact tccttcatca gatcttgttg aagcaggtat cgctgctcca gctcttgatt
     6961 tgacaactgt tcttgctgtt gcaaaaggct caaaccttaa agttgctgct caaaactgct
     7021 actttgaaaa tgcaggtgct ttcactggtg aaactagccc acaagttttg aaagaaatcg
     7081 gtactgatta cgttgttatc ggtcactcag aacgccgtga ctacttccat gaaactgacg
     7141 aagatatcaa caaaaaagca aaagcaatct ttgcgaacgg tatgcttcca atcatctgtt
     7201 gtggtgaatc acttgaaact tacgaagctg gtaaagctgc tgaattcgta ggtgctcaag
     7261 tatctgctgc attggctgga ttgactgctg aacaagttgc tgcctcagtt atcgcttatg
     7321 agccaatctg ggctatcggt actggtaaat cagcttcaca agacgatgca caaaaaatgt
     7381 gtaaagttgt tcgtgacgtt gtagctgctg actttggtca agaagtcgca gacaaagttc
     7441 gtgttcaata cggtggttct gttaaacctg aaaatgttgc ttcatacatg gcttgcccag
     7501 acgttgacgg tgcccttgta ggtggtgcgt cacttgaagc tgaaagcttc ttggctttgc
     7561 ttgactttgt aaaataatca gtaagtagca aaagctaggt ggaacagcat tcagatgtct
     7621 gttacatttt ttataggaga ggaaagattg aaaacaaaaa ttggattagc aagtatctgt
     7681 ttactaggct tggcaactag tcatgtcgct gcaaatgaaa ctgaagtagc aaaaacttcg
     7741 caggatacaa cgacagcttc aagtagttca gagcaaaatc agtcttctaa taaaacgcaa
     7801 acgagcacag aagtacagac taatgctgct gcccactggg atggggatta ttatgtaaag
     7861 gatgatggct ctaaagctca aagtgaatgg atttttgaca actactataa ggcctggttt
     7921 tatattaatt cagatggtcg ttactcgcag aatgaatggc atggaaatta ctacctgaaa
     7981 tcaggtggat atatggccca aaacgagtgg atctatgaca gtaattacaa gagttggttt
     8041 tatctcaagt cagatggggc ttatgctcat caagaatggc aattgattgg aaataagtgg
     8101 tactacttca agaagtgggg ttacatggct aaaagccaat ggcaaggaag ttatttcttg
     8161 aatggtcaag gagctatgat gcaaaatgaa tggctctatg atccagccta ttctgcttat
     8221 ttttatctaa aatccgatgg aacttatgct aaccaagagt ggcaaaaagt gggcggaaaa
     8281 tggtactatt tcaagaagtg gggctatatg gctcggaatg agtggcaagg caactactat
     8341 ttgactggaa gtggtgctat ggcgactgac gaagtgatta tggatggtgc tcgctatatc
     8401 tttgcggcct ctggtgagct caaagaaaag aaagatttga atgtcggctg ggttcacaga
     8461 gatggtaaac gctatttctt taataataga gaagaacaag tgggaaccga acatgctaag
     8521 aaaatcattg atattagtga gcacaatggt cgtatcaatg attggaaaaa ggttattgat
     8581 gagaacgagg tggatggtgt cattgttcgt ctaggttata gcggtaaaga agacaaggaa
     8641 ttggcgcata acattaagga gttaaaccgt ctgggaattc cttatggtgt ctatctctat
     8701 acctatgctg aaaatgagac cgatgctgag agtgacgcta aacagaccat tgaacttata
     8761 aagaaataca atatgaacct gtcttaccct atctattatg atgttgagaa ttgggaatat
     8821 gtaaataaga gcaagagagc tccaagtgat acaggcactt gggttaaaat catcaacaag
     8881 tacatggaca cgatgaagca ggcgggttat caaaatgtgt atgtctatag ctatcgtagt
     8941 ttattacaga cgcgtttaaa acacccagat attttaaaac atgtaaactg ggtagcggcc
     9001 tatacgaatg ctttagaatg ggaaaaccct cattattcag gaaaaaaagg ttggcaatat
     9061 acctcttctg aatacatgaa aggaatccaa gggcgcgtag atgtcagcgt ttggtattaa
     9121 gcgatgattt gaaagaggga tgtgatagta gcaccctctt tttctttgtt ttatgatagt
     9181 tcatcctcga gtaaattcaa gttcttgctc ggaaatgaag cttatatagt agattgaata
     9241 tagacaaata ccttgtgatt ggtaaaacat tttagaaatt catttacctt tcctaatcga
     9301 cttggtttca tcttatttca atctattata gtattgggga atttcttcaa accacatcag
     9361 cttggtcagt tctacctgcg acctcaaaac ttgtgctttg gtcaagctgg gtttagtttc
     9421 ctagtttgct gatggatttc cattgactat aagcatccaa ccctcttttt gtcttctaaa
     9481 gaattcttaa attatcagtc tattgcaact tttctcatat aagttctttg tcttgctatt
     9541 ggttttcctt agtagtatac taaggtagta atcattaaga agtggttaca aaaaataatg
     9601 aatgaggtaa agaaaatggt agaattgaaa aaagaagcag taaaagacgt aacatcattg
     9661 acaaaagcag cgccagtagc attggcaaaa acaaaggaag tcttgaacca agctgttgct
     9721 gatttgtatg tagctcacgt tgctttgcac caagtgcact ggtatatgca tggtcgtggt
     9781 ttccttgtat ggcatccaaa aatggatgag tacatggaag ctcttgacgg tcaattggat
     9841 gaaatcagtg aacgcttgat tacactcggt ggaagcccat tctctacatt gacagagttc
     9901 cttcaaaata gtgaaatcga agaagaagct ggtgaatacc gtaatgttga agaaagcttg
     9961 gaacgtgttc ttgttatcta ccgttacttg tcagaacttt tccaaaaagg tttggatgtc
    10021 actgatgaag aaggtgacga tgtgacaaac ggtatctttg caggcgctaa aactgaaaca
    10081 gataaaacaa tttggatgct tgcagccgaa cttggacaag cacctggttt gtaagaaata
    10141 aggaaacata gataaatctg taggaaattt cttacagatt ttttctattt tgtaagctat
    10201 tccaaaccag tctttttttg agttttttga taaaatagta ctatcagtga aaaggatgga
    10261 agcatgacta agaaaatcgt agctatttgg gcccaggatg aagagggtgt gattggtaag
    10321 gaaaatcgtc tgccttggca tttgccagca gaattgcagc actttaaaga aacaactctg
    10381 aatcatgcta tcttgatggg gcgtgtgacc tttgatggga tggggcgtcg cttgcttcca
    10441 aaacgggaaa ccttgatttt gacgcgtaat ccggaagaaa agatagatgg ggttgctact
    10501 tttcaggacg tccagtctgt tcttgactgg tatcaggctc aagaaaagaa tctctacatt
    10561 atcggtggga agcaaatttt tcaggctttt gaaccttacc ttgatgaagt gattgtcact
    10621 cacattcatg ctcgggtgga aggagatacc tatttccctg aagaacttga cttgtctctt
    10681 tttgagacag tttcaagcaa attttacgcc aaagatgaga agaatcctta tgattttacc
    10741 atccaatacc gcaagagaaa ggaagtctaa tggaacggag tttatttggt ctattcacgg
    10801 cttttctgtg tttcatctgc tttctggcag gagcacaagc ttttcgtaaa aagcgttacg
    10861 ggctttctat cctcctttgg ttaaatgcct ttaccaatct ggtcaatagt attcatgctt
    10921 tttacatgac cttattttag atagaatgac agtatagaat ggaaggaaat catgtctaca
    10981 aatagaaaaa atgatatgat ggtttattgc tcattttgtg gcaaaaacca agaagaagta
    11041 caaaaaataa ttgctggcaa caatgctttt atttgtaatg aatgcgtgga gttagctcag
    11101 gaaatcattc gagaagaatt ggttgaggaa gtcttggcag acttgtctga ggtgccaaaa
    11161 ccaattgaac tcctccatat cttgaaccac tatgtaattg gtcaagatcg tgccaagcgt
    11221 gccttggcag tggcggttta taaccactac aaacgcatca atttccacga tacacgcgaa
    11281 gagtcagaag atgtggattt gcagaagtca aacattttga tgattggccc aactggttca
    11341 gggaaaactt tccttgccca gaccttggct aagagcttga atgtaccttt tgctattgcg
    11401 gatgcgacag ctctgacgga ggctggttat gtgggtgagg atgtggaaaa tatactcctc
    11461 aaactcttgc aggttgctga ctttaacatc gaacgtgcag agcgtggcat tatctatgtg
    11521 gatgaaattg acaagattgc caagaagagt gagaatgtgt ctatcacacg tgatgtttct
    11581 ggtgaagggg tgcaacaagc ccttctcaag attattgagg gaactgttgc tagcgtaccg
    11641 cctcaaggtg gacgcaaaca tccacaacaa gagatgattc aagtggatac aaaaaatatc
    11701 ctcttcatcg tgggtggtgc ttttgatggt attgaagaaa ttgtcaaaca acgtctgggt
    11761 gaaaaagtca tcggatttgg tcaaaacaat aaggcgattg acgaaaacag ctcatacatg
    11821 caagaaatca tcgctgaaga cattcaaaaa tttggtatta tccctgagtt gattggacgc
    11881 ttgcctgttt ttgcggctct tgagcaattg accgttgatg acttggttcg catcttgaaa
    11941 gagccaagaa atgccttggt gaaacaatac caaaccttgc tttcttatga tgatgttgag
    12001 ttggaatttg acgacgaagc ccttcaagag attgctaata aagcaatcga acggaagaca
    12061 ggggcgcgtg gacttcgctc catcatcgaa gaaaccatgc tagatgttat gtttgaggtg
    12121 ccgagtcagg aaaatgtgaa attggttcgt atcactaaag aaactgtcga tggaacggat
    12181 aaaccgatcc tagaaacagc ctagaggtga ctatggaact taatacacac aatgctgaaa
    12241 tcttgctcag tgcagctaat aagtcccact atccgcagga tgaactgcca gagattgccc
    12301 tagcagggcg ttcaaatgtt ggtaaatcca gctttatcaa cactatgttg aaccgtaaga
    12361 atctcgcccg tacatcagga aaacctggta aaacccagct cctgaacttt tttaacattg
    12421 atgacaagat gcgctttgtg gatgtgcctg gttatggcta tgctcgtgtt tctaaaaagg
    12481 aacgtgaaaa gtgggggtgc atgattgagg agtacttaac gactcgggaa aatctccgtg
    12541 cggttgtcag tctagttgac cttcgtcatg acccgtcagc agatgatgtg cagatgtacg
    12601 aatttctcaa gtattatgag attccagtca tcattgtggc gaccaaggcg gacaagattc
    12661 ctcgtggtaa atggaacaag catgaatcag caatcaaaaa gaaattaaac tttgacccaa
    12721 gtgacgattt catcctcttt tcatctgtca gcaaggcagg gatggatgag gcttgggatg
    12781 caatcttaga aaaattgtga ggaaaagaaa atggcaaaaa caattcatac agataaggcc
    12841 ccaaaggcta tcggacccta tgttcaagga aaaatcgttg gcaacctttt gtttgctagc
    12901 ggtcaagttc ccctatcccc tgaaactggg gaaattgtag gagagaatat ccaagaacag
    12961 acagagcaag tcttgaaaaa catcggtgct attttggcag aagcaggaac agactttgac
    13021 catgttgtca aaacaacttg cttcttgagc gatatgaacg actttgttcc ttttaatgag
    13081 gtttaccaaa cggccttcaa agaggaattc ccagctcgtt cagctgtgga ggtagctcgt
    13141 cttcctcgtg atgtaaaagt cgaaattgaa gtcatcgcag agattggata agctagttga
    13201 agtttggtgt tgccaaactt cttttgatat aaggagaaaa agatgacaaa gaaacaactt
    13261 cacttggtga ttgtgacagg gatgagtggc gcagggaaaa ctgtagccat tcagtccttc
    13321 gaggatctag gttatttcac cattgataat atgccgccag ctctcttgcc taagtttttg
    13381 cagctggttg aaattaagga agataatcct aagttggctt tggtagtgga tatgcgtagc
    13441 cgttctttct tttcagagat tcaagctgtt ttggatgagt tggaaaatca agatggtttg
    13501 gatttcaaaa tcctcttttt ggatgcggct gataaggaat tggtcgctcg ttacaaggaa
    13561 accagacgga gtcacccact agcagcagac ggtcgtattt tagatggaat caagttggaa
    13621 cgtgaactct tggcaccttt gaaaaatatg agccaaaatg tggtggatac gactgaactc
    13681 actccacgtg aactgcgcaa aacccttgca gagcagtttt cagaccaaga acaagcccag
    13741 tctttccgta tcgaagtcat gtctttcgga tttaagtatg gaatcccgat tgatgcggac
    13801 ttggtctttg atgtccgttt cttgccaaat ccctattatc taccagaact gagaaaccaa
    13861 acgggtgtgg atgaacctgt ttatgattat gtcatgaacc atcctgagtc agaagacttt
    13921 tatcaacatt tattggcctt gattgagccg attctgccaa gttaccaaaa ggaaggtaag
    13981 tccgttttga ccattgctat gggatgtacg ggtggacaac accgtagtgt ggcatttgct
    14041 aaacgcttgg cgcaggactt atccaagaat tggtctgtta atgaagggca tcgcgacaaa
    14101 gaccgcagaa aggaaacggt aaaccgttca tgagaaaacc aaagataacg gtgattggtg
    14161 gagggactgg aattcccgtc attctaaaaa gtctgcggga aaaagatgtg gaaatcgcag
    14221 ctatcgtgac ggtggcagat gatggtggtt cttcaggtga actccgaaaa aatatgcaac
    14281 agttgacacc gccaggtgat cttcgtaatg tccttgtggc catgtcggat atgcctaagt
    14341 tttatgagaa ggtctttcag tatcggttct ctgaggatgc cggagccttt gctggccatc
    14401 cattgggaaa tctcatcatt gctggcttgt cagaaatgca gggttcaacc tataatgcca
    14461 tgcagttatt gagcaaattt ttccatacaa cagggaaaat ttatccttcc agtgaccatc
    14521 ctttgaccct tcatgcagtc tttcaggatg ggacagaagt ggctggagag agtcatattg
    14581 tagaccatcg aggcataatt gacaatgtct atgtgaccaa tgccctaaac gatgatacgc
    14641 ctctggccag ccgtcgagta gtgcagacca tccttgaaag tgacatgatt gtcctagggc
    14701 caggttccct ctttacctct attttgccca atatcgtgat taaggaaatt gggcgggctc
    14761 ttttggaaac taaggcagaa attgcctatg tctgcaatat catgacccaa cgtggggaga
    14821 cggaacactt tacagatagc gaccacgtgg aagtcttgca tcgtcacctt ggtcgccctt
    14881 ttatcgacac tgtcttggtg aatattgaaa aagtgcctca ggaatacatg aattccaacc
    14941 gttttgatga atacttggtg caagtggaac acgattttgt aggtctttgt aagcaagttt
    15001 cgcgcgtgat ttcatctaac ttccttcgtc tggaaaatgg cggtgccttc cacgatggag
    15061 atttgattgt ggacgagttg atgcgcatta tacaggtgaa aaaatgagtt tcacagtagc
    15121 agtaaaagaa gaaatcctgg gccaacacca tctgagccgg catgaattat ctgccattat
    15181 caaaatgtct ggtagcatcg gtctctcgac ttcgggcttg actttgtcag ttgtgacaga
    15241 aaatgccaaa ctggcccgtc acctctatga gtcctttctc catttctatg aaatcaaatc
    15301 tgaaattcgt caccaccaac ggagcaatct tcgcaagaat cgcgtctata ccgtttttac
    15361 agatgaaaag gtgcaggatt tgttaagtga tttgcacttg gcagactctt tctttggcct
    15421 ggaaacaggt attgatgagg cgattttatc ggatgaggaa gcaggtcgtg cctatctctg
    15481 tggcgctttc ttggtaaatg ggagcattcg tgaccctgag tcaggcaagt accagttgga
    15541 aatcagttct gtttatctgg accacgcgca aggaattgcc tcccttctcc aacagttttt
    15601 actggatgcc aaggtgcttg agcgcaagaa gggggctgtg acctatctcc agcgagcaga
    15661 agacattatg gacttcttga tagtaatcgg agccatgcag gcacgtgatg attttgagcg
    15721 ggttaagatt ttgcgagaaa cccgtaacga cctcaatcgg gctaataatg ccgagacagc
    15781 taatatcgct cggacagttt ctgccagcat gaagaccatt aacaatatca gtaaaataaa
    15841 agatatcatg ggcttagaaa atttgccagt agatttgcag gaagtggcgc aactgaggat
    15901 tcagcaccca gactactcta tccagcagtt agcagatagt ctcagcaccc ctctgaccaa
    15961 aagtggtgtc aatcacagac tcagaaaaat caataagata gcggatgaat tataaaacca
    16021 cgaatcctat gtgactcgtg gttctttttt ataaactggt agagtgtttt ggttgtactt
    16081 tttgttcagg gtcaatgtag ttgatagcgt tgttgacagc agttggagct tctccgaggc
    16141 ctgtcgcaat cagatcaatt tttccgtcat agtagcagca gtcaccgata gcatagatac
    16201 ctgcttggct ggattcctgt ttgctgttga cgataatctt gtgacggttg aggtcgagcc
    16261 cccagttttt aaggttaccg acagaagatt tgaaaccata gttgacaaag aggtggtcta
    16321 ggtcaatggt ttcagtttca tcagatttga cttttgtgat ttcaagttta tcaagtgttt
    16381 ttccatttcc aaggagttgg ctaggggcga atggtgtctt gatggttaca gatgattctt
    16441 gcaaggcttg aacactgtgt tccaaggcac ggaaattatc tctgcggtga acaagggtag
    16501 ttggtgcgat tttttcaaaa gccaaagtcc aatccacagc cgagtctccc ccaccaagaa
    16561 tcgtcacttt tttaccagcg tattgctgaa tgttagaaac gtggtagtgg atattttcat
    16621 agccctcaac cccttcaagt tccagcggac gtggtttgaa ggcaccgcca cccatagcga
    16681 tgataactgt tttagtcagg tgacttcctt tagaagttgt gatggcaaat tcttcttctt
    16741 gtttgtcaat ctcaagaacc gtttcattga gatgaatagg ggtatcaaat ccatttagct
    16801 gttcaatcaa gcggttagtc aactcttctc cagtcaggtt tgggaagcct ggtacgtcta
    16861 ggatttcctt ttcagggtag agaatagcag gttgtccacc tagctgggga agagagtcga
    16921 tgatttgaac cttggcttgg cgtaggtggg cataaaaggc tgcaaaaagc ccgacaggac
    16981 caccacccac aatggtaata tcatagagtt gagacatagt ttctcctttg ttttttctag
    17041 tcagtttatt ttatcatatt tttgttcaat gtaaaatgga ctaggatagg gaaaaaaatg
    17101 ccagaaaatg gtataatagg acgaaagtgt tttgataggg aggatatcag ggatgaattt
    17161 tcaacaatta tccaatttgc aatattggac cagtttgttt gcaagtccat ggacgatagc
    17221 tatcaatctg attgatattt tgattgttgc ttatatttta taccatttta caaaagctat
    17281 tgcaggaacc aagattatga ttttggtacg tggagttttg gtatttattt tagctcaaat
    17341 ccttgcaaat atgattggtt tgactacgat ttcttggtta atcaatcaaa ttattactta
    17401 tggggttatt gcggcggttg ttatcttctc tccagagatt cggactggtt tggaacgttt
    17461 gggaagagcg acagatttct tttccaatgc ccctattagt gctgaggaac agatgattcg
    17521 tgcctttgtt aagtctgttg aatacatgag tcctcgtaaa atcggggcct tggttgctat
    17581 tcagcgtgta cgtaccttgc aggagtatat ttcgacagga attcccttgg atgctaagat
    17641 ttctgcagaa cttctcatta acatttttat tcccaacact cccctacatg acggtgcggt
    17701 gattatcaaa gaagaacgta tcgctgtgac gtctgcctat ctgcccttga caaaaaacac
    17761 agggatttcc aaggaatttg ggaccagaca ccgggcggct atcggtttat cagaagtctc
    17821 agatgccttg acttttgtcg tatcagagga aacgggagga atttcgataa cctataatgg
    17881 aaggtttaag cacaacctaa cacttgatga atttgaaaca gaattacgtg aaatcttact
    17941 tccaaaagag gaagtaggtc ttagttttaa agaacgattg ctaggaggat ggaaacatga
    18001 aaaaaaatag tttatatatc atatcctcac tcttttttgc ttgtgtctta tttgtctatg
    18061 ctacggcgac gaattttcaa aacagtacca gtgctaggca ggtaaaaacg gaaacctata
    18121 ctaatacagt aacaaatgtc cctattgaca tacgctataa tagtgataag tattttatta
    18181 gcggttttgc ttcagaagta tcagtggtct tgactggtgc aaatcgccta tcgctagcta
    18241 gtgaaatgca agaaagtaca cgtaaattca aggttactgc tgacctaaca gatgccggtg
    18301 ttggaacaat tgaagttcct ttgagcattg aagatttacc caatgggctg accgctgtgg
    18361 cgactccgca aaaaattaca gtcaagattg gtaagaaggc tcagaaggat aaggtaaaga
    18421 ttgtaccaga gattgaccct agtcaaattg atagtcgggt acaaattgaa aatgtcatgg
    18481 tgtcagataa agaagtgtcg attacgagtg accaagagac attggataga attgataaga
    18541 ttatcgctgt cttgccaact agcgaacgca taacaggtaa ttacagtggt tcagtacctt
    18601 tgcaggcaat cgaccgcaat ggtgttgtct taccggcagt tatcactccg tttgatacaa
    18661 taatgaaggt gactacaaaa ccagtagcac caagttcaag cacatcaaat tcaagtacaa
    18721 gcagttcatc ggagacatct tcgtcaacga aagcaactag ttcaaaaacg aattaaaaat
    18781 agaaaaggat tttataacaa tgggtaaata ttttgggact gatggagtcc gtggagaagc
    18841 taacctagaa ctaacaccag aattagcctt taaactagga cgttttggag gctatgttct
    18901 tagtcaacat gaaacggaag cgccgaaagt ctttgtagga cgtgacacac gtatttcagg
    18961 ggaaatgcta gaatcggcct tggtggcagg tctcctttca gtagggattc acgtatacaa
    19021 acttggtgtc cttgcaacac cagcagtagc ttacttggtt gaaactgaag gagcaagtgc
    19081 tggtgtcatg atttctgcta gccacaatcc agctcttgat aacggaatca agttctttgg
    19141 cggtgatggc ttcaaactag atgatgaaaa agaagcagaa attgaagcct tgctagatgc
    19201 tgaggaagac actcttcctc gtccaagtgc agaaggctta ggaattttgg tagattatcc
    19261 agaaggcttg cgtaagtatg aaggatacct tgtttcaact ggaactcctc ttgatggaat
    19321 gaaggttgcc ttggatacag ctaatggagc agcttctacc agtgcccgtc aaatctttgc
    19381 agaccttggt gcccaattga cggttatcgg ggaaacacca gacggtctta acatcaacct
    19441 taatgttggt tcaacacatc cagaagccct tcaagaagtg gtcaaagaaa gtgggtcagc
    19501 tattggtttg gcctttgatg gagacagtga ccgcttgatt gctgttgatg agaatggtga
    19561 catcgtcgat ggtgacaaga ttatgtacat catcggaaaa tacctttctg aaaaaggaca
    19621 attggctcaa aatacaattg tgacaactgt tatgtctaac cttggtttcc acaaggcctt
    19681 gaatcgcgaa ggtattaaca aggcagttac tgcagttggt gaccgctacg ttgttgaaga
    19741 aatgagaaaa tcaggttaca accttggtgg tgaacagtct ggtcacgtta tcttgatgga
    19801 ttacaatacc acaggtgatg gtcaattatc agcaattcaa ttgactaaaa tcatgaagga
    19861 aactggtaag agcttatcag agttggcggc agaagtaacg atttatccac aaaaattagt
    19921 taatatccga gtggaaaacg tcatgaagga aaaggccatg gaagtgccag ctatcaaggc
    19981 catcatcgag aagatggaag aagaaatggc ggggaacggc cgtatccttg ttcgtccaag
    20041 tggaacagag cccctcttgc gtgttatggc agaagcgcct acaacagaag aagtggacta
    20101 ctatgttgat actatcacag atgtagttcg tgctgaaatt gggattgact aaatcctaga
    20161 aaactagatg aaaataaaaa attatggtac aatagcttat aatattgtag ccgatggaga
    20221 aagacatgaa tcttaaacgt gaacaagaat ttgttagtca gtatcatttt gatgcacgta
    20281 attttgaatg ggaaaatgaa aatggagctc ctgaaaccaa ggtggatgtg aatttccaat
    20341 tgctccaaca tgaccaagaa aatcaagtga cttccttgat tgtcattttg agctttatga
    20401 ttgtatttga taagtttgtc attagtggaa caatctcaca agtaaaccat atcgatggcc
    20461 gtattgtcaa tgaaccaagt gaattgaacc aagaagaggt ggaaaccttg gctcgtccat
    20521 gtttgaacat gctcaatcgt ttgacttacg aagtgactga aattgcatta gacttaccag
    20581 gaattaattt ggagttctaa tatgaaatta gctgttttca cagattcctc tgcttatctc
    20641 agtgaagaga ccttgcaaag agaagacttg tttgtcttgg atattcctgt caatattgat
    20701 ggtgaggaat atgtcgaagg catcaatctg tctgctgagg aattttacca aaaaatggct
    20761 caggcttctg aattgcctaa gaccagtcaa ccaagtattg ccaagttaga tgagatctta
    20821 acttcgctca aagaacaagg ctatacacat gccttggggc ttttcctatc ttctggaatt
    20881 tcaggttttt accaaaatat ccagtatatg gtcgatgact atgagggctt aaccattgct
    20941 ttcccagaca ctttgattac aagtgctccc ctaggcatca tggttgaaag cgtctttaat
    21001 tggcgtgatc agggcgatga ttttgccagc attcaggata agctagccat tcaaatcagc
    21061 cgtacgtcag cctttatcat ggtagatgac ttggatcatt tggtaaaagg tggacgcctt
    21121 tcaaatgggg ctgccatttt gggcaatttg cttagcatta agccaatcct ttattttaac
    21181 gatcaaggtg tgattgaagt ttacgaaaaa gttcgtactg aaaagaaggc caccaagcgc
    21241 ttaattgaaa ttatcaagga aacaacggct tcaggtcaat accgggtcat tgtcattcat
    21301 gggaatgctc ctgaaaaggc tgaagaattg cgtcagcact tgcttgattt tggcttgggt
    21361 tcggatgttt cacttgctac atttggtagt gtcattggaa cgcacctagg agcaggaagt
    21421 attgctctgg gttatattcc agtgatttag ttggcgcttc taggctagtt aagatgcctt
    21481 gtatcttaga aattgaacac ggactacctg cctcttgaaa aaagatgcct gtcttgcctt
    21541 tcgtggaaag tcagtgccat tccctatttt tcagaggcag ctaaggtcct ttgtatcttg
    21601 ataattgaac acgcctggaa ccctgtgtga aaaagatagt tcttccaaga agtagacact
    21661 tcttgattgg aattcctatt ttcactttgt gttcttacgg actttgtatc ttagacagga
    21721 gtagagatga gtattcgagt aattattgcc ggttttaagg gaaagatggg ccaggctgct
    21781 tgtcagatgg tattgactga tccagacttg gacttggtgg cagttttgga tccttttgag
    21841 tctgagtcag aatggcaggg tattcctgtt ttcaaggata aggctgattt agctggtttt
    21901 gaagcggatg tctgggtaga ttttactact ccagctgttg cctacgaaaa tacacgtttt
    21961 gctcttgaaa atggctttgc tccagtagtt ggaacgactg gtttcacgag tgaagaaatt
    22021 gcagagctaa aagaattttc tcgtgcccaa gacttgggtg gcctgattgc ccctaacttt
    22081 gccttgggtg ctgtcttact catgcaattt gcgacgcagg ctgccaaata tttcccaaat
    22141 gtggagatta ttgagctcca tcatgacaag aaaaaggatg ctccgagtgg aacagccatt
    22201 aaaacagctg agttgatggc agaggttcga gagtccattc agcaaggcgc agcagatgag
    22261 gaagagctga ttgctggtgc tcgtggtgct gactttgatg atatgcgcat ccactcagtt
    22321 cgtttgccag gcttggtagc tcatcaggaa gtcatctttg gcaatcaggg agaagggttg
    22381 accctccgtc atgactccta tgatcgcatc tccttcatga caggagtcaa tttgggaatt
    22441 aaagaagttg tcaagcgtca tgagcttgtc tatggattag aacacttatt atgagattaa
    22501 cgcaaatgcc ttctgaattt cagaaggctt taccagtatt agaaaaaatt aaagaagcag
    22561 gctttgaggc ttattttgtt gggggctctg ttcgagatgc ccttctccat agccctatcc
    22621 acgatgtgga tattgcgacg tcttcttatc cagaagagac caagcagatt tttccgcgaa
    22681 cagccgatat cggaatcgag catggaaccg tcttggtctt agatggggat gaggagtatg
    22741 aggtaacaac ctttcggaca gaggatgtct atgtggacta tcgcagaccc agtgcggttt
    22801 cctttgtacg atcgctagaa gaagacctca aacgccgtga tttcacagtc aacgcctttg
    22861 ccttggatga gacaggagaa atcgttgact tgttccatgg tttagaagat ttggaaaagc
    22921 aagtcttgcg agcagttgga gtggctagtg agcgtttcaa cgaagatgct ttacggatta
    22981 tgcgtggttt ccgttttcag gctagtcttg gttttgcact tgagccagaa acatttaaag
    23041 ctatgaagac cttgacgccg cttttggaga aaatttctgt agagcgtacc ttcgtcgagt
    23101 ttgataaact cttgctggct ccattttgga gaaggggctt ggcttccatg attgagagtc
    23161 aagcttatga ctatctccct gatatggcat ctagccagga caagctcaac agactgtttg
    23221 atttggagac tgattttact tttgaatcct ctgaacaagc ctgggcggct ttactatggg
    23281 ctttggagat tgaaaatgcg cagtcatttt tgaaatcttg gaagacctca cgccagtttg
    23341 ccaagcaagt tcaggatttg ctgattattt tggctctgcg tgaaaatgga gaattgagta
    23401 agcgagattg ttatcgcttt gacatagatt tgcttttaca ggctgaaaat cttcgtcaag
    23461 ctcaaggaaa agaagtcaac ccacaagcca tcacagaaaa ataccaaagc ttgactatcc
    23521 atgacaagaa agagattcag attaatggcg atattttgat caaggaatat ggctatcagc
    23581 caggcccaga cttgggagag attttaacag agattgagtt tgccattgtc gatggagaat
    23641 tggagaataa tcgtgaagcc atccatgctt acctgaggga gaaaaaatga gtgattttat
    23701 cgttgaaaaa ctaagtaaat ccgttggtga caagaccgtt tttagggata tttcctttat
    23761 tatccatgac ttagacagaa ttggtttaat cggtgtcaat gggactggca agaccaccct
    23821 tttggacgtc ctttctggtg tttctggatt tgatggggat gtcagtcctt tttcagctaa
    23881 aaatgattac cagattggtt acttgactca ggatcctgat tttgatgata gaaagacagt
    23941 tttggatacg gttctatcta gtgaactcaa ggaaatccag ctcattcgtg agtatgaatt
    24001 gattatgctc gactatagtg aggacaagca ggcgcgtttg gaacgtgtca tggcagagat
    24061 ggactctctc caagcttggg aaatcgaaag tcaggtcaag accgttctta gcaaattggg
    24121 cattcaagac ttatctactc ctgttgggga attgtcaggt ggtctgagaa gacgggtaca
    24181 gttggcacaa gtcttacttg gcaaccacga cctcttgctt ttggatgagc cgaccaacca
    24241 tctggatatt gcgattattg agtggctgac cctctttttg aaaaattcta agaagaccgt
    24301 cctttttatc actcacgatc gttatttctt agacgctttg tcaacacgga ttttcgagtt
    24361 ggatcgtgca ggcttgaccg agtaccaggg caattaccag gactatgttc gcctaaaggc
    24421 ggaacaggat gagcgcgacg cggctcttct tcacaaaaaa gaacaactct acaaacaaga
    24481 actggcctgg atgcgcagac aaccgcaggc gcgtgcgacc aagcagcagg cccgtatcaa
    24541 tcgattccat gatctgaaaa aggaagtttc aggcagtagt gctgagacag acttgaccat
    24601 gaactttgaa accagtcgga ttgggaagaa agtcatcgag tttcaggctg tttcctttgc
    24661 ctatgaaaac aagcctattt tgcaagattt taatctcttg gtgcaggcca aagaccgtat
    24721 cggaatcgtt ggggacaatg gtgttggaaa atcaaccctg ctcaacttga ttgcaggaag
    24781 tcttgagcca actaaaggac aagttgtgat tggggaaact gttcgcatcg cctatttctc
    24841 tcagcaaatt gagggtttgg atgaaagcaa gagagttatc aattacctgc aggaagtggc
    24901 agaagaggtc aagaccagtg gcggttctac gacttcgatt gcagagttgc tggagcagtt
    24961 ccttttccca cgttcgacgc atggaacctt gattgagaaa ttgtcagggg gtgagaaaaa
    25021 acgtctttat ctcctcaaac tgcttttgga aaaaccaaat gttcttcttt tagacgagcc
    25081 aaccaatgac ctagatattg caactttgac agtcttagag aatttcttgc aaggttttgc
    25141 aggtcccgtt ttaacagtca gtcacgaccg ctatttcttg gataaggtag cgaccaagat
    25201 tctcgctttt gaggatagca agattcgtcc tttctttggt cattacaccg actatcttga
    25261 tgaaaaagct tttgaaacag atatggccaa tcaagtgcaa aaggtcgaaa aggaaaaagt
    25321 ggtcaaggtt cgagaagaca agaaacgtat gacctaccaa gaaaagcagg agtgggcaag
    25381 tattgaaggt gatattgaaa ccttggaaaa acgtatcgct gctattgaag aggaaatgca
    25441 ggctaacggc tctgactttg gtaagctggc tactctccaa aaagaattgg atgagaaaaa
    25501 tgaagcactc cttgaaaaat acgaacgcta tgagtatctc agtgaatttg atagttaaga
    25561 ctattcgaaa atcaaagtgc aaactatgtc ggcttcacat tgccgactca agtacagcct
    25621 gtgaccggct tcctaatttg cactttgatt tttattgagt ataaaagaat agaaaaatcc
    25681 cgtctcatga aaggatttct ctattctttt ttggtttctt gatgaaagat attttgccag
    25741 gtttcgtggc gacgccagat actggtatgg atgataccat ctaactcata gcagatgagt
    25801 ttggtttttt gactgatgga agtaatttga atgtccttga tagcagaatt taactctttt
    25861 ttggtcttat aagcctcttt atccatctgc tctccatctt ggcgaatata gacaaaatca
    25921 tcagtcaaga gttcttctag ttggtttcct tggtcaatca attgttcacg cataagcaat
    25981 tttttataga cattgtctaa aatttcatcc tcagagatag gtgctggttc gatatggaca
    26041 tcggtatcaa agacgccaaa acgctcctcc agcatagact cgacctgatc cgcgatttca
    26101 tggctttcaa aaacagacaa gtcaggattc atctctagtg taatatccag gtagatgttg
    26161 ctaccgtagg tgcgacctct ttgcgatttg accttgctga ttttgggaat ttccatgata
    26221 gccttttggt agtcctcgag caggcggtcg tcaaagccat ctgaaagact aaaggaagac
    26281 tcgatgaaga tatcataggc agtcttcaag ataaagaaag tgatgatgat agcaaccagt
    26341 ttatccacaa tcggataatt gaaactacta gctaggatgg caatggcggt tccaagtgag
    26401 gtaacagcgt cagaaagatt gtccttagca gctgccttca gcgccttgga gttggatttc
    26461 ttactgaggc gagtattgta gagatagacc acaaacataa tcgctgcaga aatgattcct
    26521 agagttgcac caagaggatc aatgaccgtt tcttcccgac tgagaatctt ttgaatggta
    26581 tctcttagaa catcgaaacc gacatagaac atgatgatag aagtgatcaa gcttgccaaa
    26641 tcttcaatct tccaatgacc aaaacggtgg tcacggtctg caggctggcg cgccatccga
    26701 atcccgatta agagggccac atttccaatg atgtccgata cgttattaaa accatcggcc
    26761 accaaactgg atgaatgaag gagatgacca gctgctaatt tggctgcaga caagatcaaa
    26821 taggtcgaaa tactgataat ggctccacgc tcagctaact tgagatttga gatagattgc
    26881 ttcattcaac ttcctttcta gtcacatagc attctaccat tatactggtt ttcaaggaaa
    26941 aattcaaata aggcagtttt tactctttga aaactcccca aaatttggta taatggtact
    27001 actcaaggga gtagctggca gaaacctgtg atagtgtcgt cattccgaat tttatgctga
    27061 aaagtatgct ttccggccct atcttaaaca gcgagacttg ttatgattaa caggtctctt
    27121 tttgtttgtc gtgaaaagat atgacagaaa gagagtctgt tttgcacaca atgtcaagga
    27181 ggagacacat gtcaaaagaa caaaaacgcc aagcgtttta tactcagagc cctgaagagg
    27241 tcttgcaggc tgtggatgcg accgagcaag gtttgtcatc aagtgaggcg gaaaagcgcc
    27301 ttgctgaatt tgggcacaat gaactcgaag aaggcgagaa acgatcaatc ttggtcaaat
    27361 tcatcgagca atttaaggat ttgatgatta tcatcctagt tgcggcagca atcttgtcag
    27421 tcgtgacttc tggtggggaa gatatcgcag atgccattat catcctagct gtggttatca
    27481 tcaacgctgc ctttggtgtt taccaagaag gaaaagctga agaagctatt gaagccctca
    27541 aatccatgtc tagtccagtt gcccgcgttc ttcgtgatgg acacatggca gagattgact
    27601 ctaaagaatt ggtaccaggc gatatcgttg cccttgaagc aggtgacgtg gtaccagcgg
    27661 acctacgttt gatagaagcc aactctctta aaattgaaga agcagccttg acaggtgaat
    27721 ctgtaccagt cgaaaaagac ttgtcagtcg agcttgcgac agatgctggt attggtgacc
    27781 gtgtcaacat ggccttccaa aactcaaacg taacctatgg tcgtgggatg ggtgttgttg
    27841 tcaatacagg tatgtacact gaagttggtc atattgctgg tatgcttcaa gatgcggatg
    27901 agactgatac accactcaaa caaaatttga acaacctttc taaggtcttg acctatgcta
    27961 tcttggtcat tgcccttgtt acttttgtag tgggtgtctt cattcaaggg aaaaatccac
    28021 ttggtgagtt gttgacttct gttgcccttg ccgttgcagc cattccagaa ggacttcctg
    28081 ctatcgttac catcgttctt tctcttggta ctcaagtttt ggccaaacga cattccatcg
    28141 ttcgtaagtt gccagcagtt gaaacacttg gttcaactga aatcatcgct tctgataaga
    28201 ctggtacgct gactatgaac aagatgacag tcgaaaaagt cttctacgat gcggttctac
    28261 atgactcagc tgatgatatt gaactaggtc ttgaaatgcc actacttcgt tcagttgtct
    28321 tggccaatga tacgaaaatc gatgtggaag gtaacttgat tggtgaccca accgaaacag
    28381 cctttatcca atatgccttg gacaagggct atgatgtcaa aggtttctta gagaaatatc
    28441 ctcgtgtagc tgaattgcca tttgactctg accgtaagct catgtcaaca gttcacccat
    28501 tgccagatgg tcgtttcctt gtagcagtca agggtgcgcc agaccaactc ttaaaacgtt
    28561 gtcttcttcg tgataaggct ggggatattg ctccgattga tgagaaggtt acaaatctca
    28621 ttcatacaaa caattctgaa atggctcatc aagccttgcg tgtccttgca ggtgcttata
    28681 agattatcga tagtattcca gaaaatctca cttctgaaga gcttgaaaat gatttaattt
    28741 ttactggttt gattgggatg attgaccctg aacgtcctga agccgctgag gctgttcgtg
    28801 tggctaagga agcgggaatc cgtccaatta tgattacagg tgaccaccaa gacacagcgg
    28861 aagccattgc caaacgtttg ggaatcattg acgcaaacga tacagaaggt cacgttttaa
    28921 ctggtgctga actcaatgaa ctgtcagatg aagaatttga aaaagtcgtt ggtcaatact
    28981 ctgtttatgc ccgtgtgtct ccagaacaca aggttcgtat cgtcaaggct tggcaaaaac
    29041 aaggtaaagt cgttgccatg acaggtgacg gtgtcaatga cgcgccagct ctgaaaacag
    29101 ccgatatcgg tatcggtatg ggaatcactg gtacagaggt ttctaagggg gcttctgata
    29161 tgattcttgc agatgataac tttgcgacta ttatcgtcgc agtggaagaa ggacgtaagg
    29221 tcttctcaaa cattcaaaag actattcagt acctactttc tgctaatact gctgaagtat
    29281 taaccatctt cctatcaacc ttgtttggtt gggatgtctt acagccggtt catcttttgt
    29341 ggattaactt ggtaacggat accttcccag ctatcgctct tggtgttgaa cctgcggaac
    29401 ctggtgtcat gaatcataaa ccacgtggac gcaaggcaag cttcttctca ggtggtgttt
    29461 tgagttctat catttatcaa ggtgtactcc aagcagctct tgttatgagt gtttatggcc
    29521 ttgcgattgc ttacccagtt catgtgggtg acaatcatgc tattcatgca gatgccctaa
    29581 cgatggcctt tgcaaccctt ggtttgattc agctcttcca tgcctacaat gtcaagtctg
    29641 tctaccaatc catcttgaca gttggcccat tcaagtctaa gacctttaac tggtccatct
    29701 tggtatcctt tatccttctc atggcaacaa tcgttgtaga accgcttgaa ggaatcttcc
    29761 acgtaaccaa actagacttg tcacaatggg gaattgttat ggctggaagc ttctcaatga
    29821 ttatcatcgt cgaaatcgtt aagtttattc aacgcaaact cggttttgac aagaatgcga
    29881 tttaagggag gtttcggatg tcagcatatc aattaccgac cgtatggcag gatgaagcta
    29941 gtaatcaagg agcttttacg gggctaaaca gaccaacagc aggtgcccgt ttcgaacaaa
    30001 acttgccaaa aggagaacaa gcttttcagc tttattcact gggaacacca aatggtgtga
    30061 aggttactat cttattggaa gaattactag aagctggttt taaggaagcg gcttacgact
    30121 tgtataagat tgctatcatg gatggggatc aattcggctc agactttgtg aagctcaatc
    30181 caaattccaa gattccagcc ttattggacc agtcaggtac tgaaaacgta agagtctttg
    30241 agtctgctca tattcttctt taccttgctg agaaatttgg agccttttta ccaagtaatc
    30301 ctgtggaaaa ggtagaagtt ttgaattggc tattctggca agcaggtgca gcaccttttc
    30361 taggtggggg atttggacat ttcttcaatt atgctcctga aaaattggaa tatcctatta
    30421 accgttttac gatggaagtg aaacgccagt tggatttatt ggataaggaa ttggctcaga
    30481 aaccttatat tgcaggcaat gactatacga ttgcagatat tgctatctgg tcttggtatg
    30541 gacagttagt tcaaggaaat ctttaccaag gttctgcaaa attcttggat gcctcaagtt
    30601 atcaaaatct agtaaaatgg gcagaaaaaa ttgccaatcg tccagctgtt aagcgtggct
    30661 tggaagtaac ttatacagaa attaaatagc aaaaaagtca tcttcggatg gctttttgct
    30721 acagttgagg gaaagggttt gcagttatcg gcttttgtgc tataattgct ataatatagt
    30781 aaaaagcaag aggagtgtga ggaagtggaa aagaaaattg tgaaggatat cttattttta
    30841 tctcaagtgt ctcatccggc aagtcaggag gacctttatc ttgccagaga tttgcaggat
    30901 acactcttag caaatcgtga tacctgtgtt ggtctagctg ccaatatgat tgggatgcag
    30961 aagcgcgtga ttatctttaa tcttggcttg gttcccgtgg tcatgtttaa cccagtgctt
    31021 ctgtcctttg aaggatccta tgaggcagaa gaaggctgtt tgtccttggt aggtgtgaga
    31081 tcaactaagc gttatgaaac cataaggctt gcctatcgtg acagcaagtg gcaggaacag
    31141 accattacct tgacaggctt cccagctcag atttgccagc atgagctgga tcacttggaa
    31201 ggacgaatca tttaggagga aagcaaatga aacgaatagt ctttgaactt atttttatcg
    31261 caacgacctg gtatatcttt ttaccgcccc ttaacctgac cagctgggaa tttctcttct
    31321 tcctctgtgg gcatttgtta gttgtggcaa tcttatttgg ctttggcaag gggataaacc
    31381 ttgtcaaaac ggttcatgtg cgccacggta aggcggaagc tgccttaaat cttgagggtt
    31441 tcaaaatcaa tcggttaggg aaaattctgt tagcttcgat tggaggaatt cttctcttgg
    31501 cagctttggt ttccttggta acttctagca tgtttcaggc taaaaattat gccaatgtag
    31561 tcacggttac ggaaaaagac tttactgaat ttcctaagag tgacaccagt aaggttccta
    31621 tcctagatag aagtactgct gaaaaaattg gagaccgcta cttgggttcc ctaaccgata
    31681 aggtgtcgca atacgtagcg gcagatacct atacccaatt gacaattgat gggaaacctt
    31741 atcgggtcac accactagaa tatgcagacc ctatcaaatg gtttaacaat caagccaagg
    31801 gaatcggtga gtatatcaag gtggacatgg taactggaaa tgcggatttg gtggacttga
    31861 agacaccaat caagtattca gactcggagt attttaaccg tgatgtcaaa cgtcacctgc
    31921 gcttgaagta cccgaccaaa atctttaaaa ctccatcttt tgaggtggac gatgagggca
    31981 atcctttcta tgtagcaacg gtttaccaaa agcaatttgg acttgctgtt cctcgtcctg
    32041 cttcagtcat tatcttggat gctacaaatg gagaaaccaa ggaatacagc ttatcagatg
    32101 ttccagaatg ggtggacagg atctatccag cagaggaaac cattgagcaa atcaactaca
    32161 acggcaagta caaggacggt ttcttgaatg ccatgatttc caagaaaaac gtgacccaga
    32221 cgaccaatgg ctataattac ttgtctatcg gtaatgacat ctatctctac acaggtgtga
    32281 cgtcggctaa tgcggatgag agtaatcttg gtttcatcct tgaaaatatg cgaacaggag
    32341 aaattactaa gtatagcttg gcttctgcga cagaagaatc agcccgtgaa tcagcagaag
    32401 gtgctgttca ggagaaatcc tacaaagcaa ccttcccaat cctcatcaac ctcaatgaca
    32461 agcctctcta catcatgggc ttaaaggaca atgctggctt ggtcaaagag tacgccctgg
    32521 tagacgcagt cgagtaccaa aatgttatcg ttgctactac agtggaagag atgctcagca
    32581 agtatgccaa taaaaacgac cttgaaattg acaatgcaac gacagaaagc atcaagggag
    32641 tagtagcaga cctcaaatca gctgttatca agggagacac tgtctacttc tttaaagttg
    32701 atggcaagat atacaaggta aaggcttcag tatccgatga ccttccttac cttgaaaatg
    32761 gtaaaacctt cgaaggtcaa gtaggaaaag acaattatct caagaccttt aaagtacaat
    32821 aatactcttc gaaaatctct agctctatct gcaacctcaa aacagtgttt tgagcagcct
    32881 gcggctagtt tcctagtttg ctctttgatt ttcattgagt ataaaaaagg tttatttaat
    32941 aaatatatgt tataataagg taaattaagc ctaatggagg acaaagaaat gggtttttat
    33001 ttgatggttg cttcaatgct actcggactg ctcgctttga agataggttt ttctcagttc
    33061 aaggagaaaa aagataaatt cttatctatc ttaacaagtt tagctggcac agcccttgtt
    33121 ctcgtggctg tctggttagg atggcccaaa taaatagaaa aacctcggtc aaaccgaggt
    33181 ttttcaaatg aatttcttgg ttgtggctaa aaaatatgct acactatcaa tatgaaaatt
    33241 ttaatcccaa cagcaaaaga aatgaacaca gacttgccaa gtatcgaggc aattccttta
    33301 aaaccagaaa gtcagaccgt gcttgatgcc ttggctctct attctgccag tcaattggag
    33361 agtttctaca aggtatcagc tgagaaagcg gcggaagaat ttcaaaatat ccaagctttg
    33421 aaaaggcaaa ctgctcaaca ctatccagcc ttgaaacttt ttgatgggct tatgtaccgc
    33481 aacattaaga gagataagct gaccgaggcg gaacaagatt atcttgaaaa tcatgtcttc
    33541 attacctcgg ctttgtacgg tgttgttcca gccttgtcac ccatggctcc tcaccgtttg
    33601 gattttttga tgaaattaaa agtcgctggt aagactttga agagccattg gaaggcagtc
    33661 tatgatgaag ctctgaagaa ggaagaagtg attttctctc tcttgtcatc agagtttgag
    33721 actgtatttt ctaaggaaat cagagcaaag atggtgacct tcaaattcat ggaggataga
    33781 ggcggtcagc tgaagattca ctcaactatc tccaagaaag cgcgcggggc ctttctaaca
    33841 gctttaatag aaaatcaagt acaaactgtg ggggaagcac gtcgcttgaa ctttgctgga
    33901 tttgtttacc gagaagattt gtcacaacca cagggattgg tttttgtaaa ggaagtatag
    33961 aaatcagata agagaaaagt cagcacgatt atgctgactt ttttgataac ttataaagta
    34021 ttggacaaat gaatctcatt tgataaggtg ttaagatgat taaccaaatt catgattgac
    34081 agatgatttc ggcaaataaa cttcttggta atccctgaaa tatagaaatt agacacgatg
    34141 atatcgtaat ctgtctgatt taaaatctca ggacttgttt ttaattcatc ccaaatatcg
    34201 aaggtaaagc ggttattaca gtaataggaa agcatatcaa caaaggtaag ggatatagca
    34261 tggtcaaagt tactgataat caaaaccttg ataggtggtc gattttctaa caactgagta
    34321 gagaggtttt cagcatgggt taagatggtg tacatcaagt gttccaactg ttcaggatgg
    34381 tcatgctgtc caattgcctg acggtattga gccagttctt gacgcgcact tcccataaag
    34441 tctgggaagt aaccttcaaa ttttttaatc gtcaatgctt tttgttcaaa taagattggg
    34501 gtagaaaaga tttcctgtct ttcaaagaaa gcagtgttgt gtaaatgcca aatcagctca
    34561 tcgtggttag taaaagtaat cttatattgt tttgataggc gttctaatat ttgactaagt
    34621 aattggtaag agtatcttgc ttgactgtta tcttctaaag agttgaagaa ttcttgagga
    34681 tctaggaaaa tatcattctg aagaaaagaa ataaaaattt gagcgattgt atctggagtc
    34741 atttcaagcc caaaatgttt agagaaataa gcaagtgtct cctggaaatt tggaatgctc
    34801 tttaaaagag gataatattc tttgtaaaag tggttgggga ggtcaatgaa gtgaccattt
    34861 ttaaccctgt ggatactgat agctatcatc agtttgtaca ttctataaat tgaaaatcgc
    34921 attggataat tcgtgatttt atagaagaag tcagcaaact ctgttaaatc ttcctcagga
    34981 aggtcaggaa aaggccaatc taggaaataa tagcgctcag aaaagtattg ggcaaaaaag
    35041 taacgaatat cgatctcatt tccacgtatc tcggatggag taaaagataa ttcaatttga
    35101 aactgagatg aaagaacctt tgtaatattt ctgcccagtc gatagaggtt ggcactacta
    35161 aagtaaagat tttctattgt tcgataaata ggtagcccct cattgaagaa catatactct
    35221 agtaattgaa aagattgaga attagcaaga aaatattgat aaatatcttc tacactagtg
    35281 ttaacttcta aatctatcat gattccgtta acagaagatt ggatgttaat agatggaaag
    35341 gcaatacgta attcatttaa atcacttttt aagattcgtt ctgtacatcc taatttttcg
    35401 gctagttcat gtaatttcat ccaattacga ttctgaatta gggtttccat taagcgtaat
    35461 tgtctttgaa cttttgtgga taaaaggttt ctcatacggg tatttcctcc ataattcatt
    35521 atatcttttt ttgtctaagt agtataaaaa aattagagca agaaaattgt cgttagtaat
    35581 cggaaaaatt agaataatat aagtggcttt taagtgcaaa aacagaatat attaattgtt
    35641 cagaaaatat tgattaatgc ctcaaatagt tgtacattgt aaactataaa atactatcta
    35701 ggagtttaaa atgaaacatt cacataaaaa atcatttgac tggtatagca tgcaacaacg
    35761 ttattctatt cgtaagtatt actttggtgc agttagtgtc ttgctcggta ccgctttggt
    35821 attaggtgca gcagctagtg tccaaacagt acaagcggaa gaaaacaaac aagaaactac
    35881 caatagtatt tctgttggta ggggagaagc agctactaaa ccagcagagg tttctgcgtc
    35941 taataaagag aaaacctatg cagctccaac tgtagctaat ccagtagaaa cgactccagt
    36001 taaaactgga gaagttacta aaccagcaga aaaagttgaa gaagcaaaag acaaaaaaga
    36061 ggaagtaacg catcaagatg ctattgacaa gtcaaaattg ttaacggctc tttcgcgtgc
    36121 taaaaagtta gaaagcaagt tatatacaga agcaagtgct gctaacttgc aaacaagtat
    36181 ccaagctggt caaagcttgc ttgggaaagc agatgcatct gaagctgaat tatcagcagc
    36241 agagtcatct attcaatcat ctattattgg tctagaactt cgttctaact ctaacaaagg
    36301 aactgtatca gaaactcctg tagcgaagaa agctaatata gttgaagcga aggaagagac
    36361 taatccagct gcaacaactg atcgttcagc tgttgatagt gctgttttgc caactagcac
    36421 agctgccaaa gtagaaacaa cttcagctcc agcatctatt aatgaaatct tgaaaccagg
    36481 tttgagcctt tctgatgctc gccaaaatcc agctatccgt aaggaagatg ttgatagagg
    36541 gtatagtggt tttagagcgg cgcctgctcc aacgaaccaa ccagtaacta atctaggaaa
    36601 taatactgtt ttcactgata ttagcagagg ttctcatact ttccgtggta atggaaattc
    36661 acgtcgtgga aacccgattc attttgatgt aacaacaact agagttagaa atagagtaga
    36721 tttctcaatc agatattcag gaccaggtga gtttgttaat aacaacttta tcttagacaa
    36781 aggaaatgga ttcggagaac cttctcgtgc aactgtttca acaccaaaat taagagacca
    36841 agttaaaaac attagtaagg gtggaaactt tgtttctcat tcaggctaca gtttagaagc
    36901 tgcagtagaa acaagcacca atcaaacaat taagtttagt ttacctatag ccaaccctaa
    36961 tggtgatctg tctgttcgat taagacctgt tacttttaat gtaggtcaag gggaatcagg
    37021 atctgcaact agtaatgatc catatagtaa ctctaactat tacttcagag aaaaaccact
    37081 cctcttggat gcaaatccaa atggtggtac taataataag actgtttcag aagacattga
    37141 cttccaaact gtctatcttc caactagtaa gttaccagaa ggtcaaacta gattagttcg
    37201 agaaggtgaa aaaggacaac gtcaaatcat ctataaagtt catcaattgg gtaacgaaac
    37261 aatcctagga ttgccaatta gtaattcagt tattaaagaa gttaagccac gtattatgca
    37321 aattggtgtg gctaaagatc taatcgatac agtaaaacca cgtgttgatc aaaataaagt
    37381 cggtgataca aataacctca ctttctatct tgataacgat ggaaacggtg tttatactga
    37441 gggcgtagac gaacttgttc aaaaaattgc tattaaagat ggtgctaaag gtgaaaaagg
    37501 agaccgaggc gaacgcggtc taactggagc tcaaggagct aaaggtgaaa aaggagaccg
    37561 aggtgaacgc ggtctaactg gagctcaagg agctaaaggt gaaaaaggag accgaggtga
    37621 acgcggtcta accggagctc aaggagctaa aggtgaaaaa ggagaccga
//
LOCUS       DAVBCB010000014        37452 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVBCB010000014 DAVBCB010000000
VERSION     DAVBCB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.014"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..777)
                     /locus_tag="VQU28_000698"
     CDS             complement(<1..777)
                     /locus_tag="VQU28_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2476085.1"
                     /translation="MSKETVVYTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVY"
     gene            complement(873..2183)
                     /locus_tag="VQU28_000699"
     CDS             complement(873..2183)
                     /locus_tag="VQU28_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HET2476086.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(2310..3305)
                     /locus_tag="VQU28_000700"
     CDS             complement(2310..3305)
                     /locus_tag="VQU28_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2476087.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFGGDKTTKKI"
     gene            complement(3667..5148)
                     /locus_tag="VQU28_000701"
     CDS             complement(3667..5148)
                     /locus_tag="VQU28_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2476088.1"
                     /translation="MRDLLSKKSHRQLELLELLFEHKRWFHRSELAELLNCTERAVKD
                     DLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNEGCQ
                     AESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQYFSE
                     KYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGHFME
                     VDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFIDESL
                     FMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELFTEF
                     ILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHTKHL
                     VINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLEDSS
                     YDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            5591..5755
                     /locus_tag="VQU28_000702"
     CDS             5591..5755
                     /locus_tag="VQU28_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2476089.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            5839..6405
                     /gene="amaP"
                     /locus_tag="VQU28_000703"
     CDS             5839..6405
                     /gene="amaP"
                     /locus_tag="VQU28_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HET2476090.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            6417..6587
                     /locus_tag="VQU28_000704"
     CDS             6417..6587
                     /locus_tag="VQU28_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HET2476091.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            6626..7234
                     /locus_tag="VQU28_000705"
     CDS             6626..7234
                     /locus_tag="VQU28_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HET2476092.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            7265..7468
                     /locus_tag="VQU28_000706"
     CDS             7265..7468
                     /locus_tag="VQU28_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2476093.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(7578..7856)
                     /locus_tag="VQU28_000707"
                     /pseudo
     CDS             complement(7578..7856)
                     /locus_tag="VQU28_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895264.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8066..8341
                     /locus_tag="VQU28_000708"
     CDS             8066..8341
                     /locus_tag="VQU28_000708"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2476094.1"
                     /translation="MKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDI
                     SNHHDSESFFANLGCSRQRRLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(8619..9389)
                     /locus_tag="VQU28_000709"
     CDS             complement(8619..9389)
                     /locus_tag="VQU28_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HET2476095.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(9404..10252)
                     /locus_tag="VQU28_000710"
     CDS             complement(9404..10252)
                     /locus_tag="VQU28_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HET2476096.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVPNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDRFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(10249..11724)
                     /locus_tag="VQU28_000711"
     CDS             complement(10249..11724)
                     /locus_tag="VQU28_000711"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HET2476097.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYVSDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTSLKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(12047..13402)
                     /locus_tag="VQU28_000712"
     CDS             complement(12047..13402)
                     /locus_tag="VQU28_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2476098.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(13412..13729)
                     /locus_tag="VQU28_000713"
     CDS             complement(13412..13729)
                     /locus_tag="VQU28_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2476099.1"
                     /translation="MDESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(13746..14063)
                     /locus_tag="VQU28_000714"
     CDS             complement(13746..14063)
                     /locus_tag="VQU28_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2476100.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(14076..15761)
                     /locus_tag="VQU28_000715"
     CDS             complement(14076..15761)
                     /locus_tag="VQU28_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 16 glycosylhydrolase"
                     /protein_id="HET2476101.1"
                     /translation="MKNKKLISSLFILFLLIFIHVKVFANENLIKNSNFEQGELFWNS
                     RNRGIINSNESYSNGHYSGMIPSTAINDNRADGYIGQVIDIEPNEDYLVSAFAKVDIS
                     GAEGYFTARWFDNNKQGEIVQNNIGEAVDQTVNTTEWKKYMFSFNSGKHDKVLIQLVK
                     WSEDDTTKKSNIFIDNVEMYQLSKGNSYKKIWRDDFDGEQLNKKYWGYELGSIRGWEQ
                     QHYVRSDENVFLRSGNLVLRATNRSKEDQYFNPRNNHRKVVYNSGSVRTHGKVEFLYG
                     KLEMRAKLPKGQGVFPAFWTLGSDFTLDGKINPVQGRGWPSTGEIDIMELVGERNSNG
                     SGNKTVYQTLHYGQSDNDDGKFAGRGTAFKLSSGNFNDSYHTFSIDWYKDYIVWMVDN
                     HIVRKVYYGSDNIAKKIFNRPQYVQLNLAMGGSWPGVVDNNLSGTEFVVDYVSYSRND
                     LQQHQAEEYYSQSPKINGAKDIVINRGDVPDLLKGITTNSGYELDYSIENEPMFNNFG
                     GNTSVDLLIRNKTEKEKISQMPPGVYNLHYTSRPKHLDYESKVDRKTVTLTIK"
     gene            complement(15766..16662)
                     /locus_tag="VQU28_000716"
     CDS             complement(15766..16662)
                     /locus_tag="VQU28_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HET2476102.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(16665..17927)
                     /locus_tag="VQU28_000717"
     CDS             complement(16665..17927)
                     /locus_tag="VQU28_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2476103.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQGALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPSWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            18207..19385
                     /locus_tag="VQU28_000718"
     CDS             18207..19385
                     /locus_tag="VQU28_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2476104.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(19613..20059)
                     /locus_tag="VQU28_000719"
     CDS             complement(19613..20059)
                     /locus_tag="VQU28_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476105.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLPLQ
                     ALEGVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSGLKLE"
     gene            20129..20788
                     /locus_tag="VQU28_000720"
     CDS             20129..20788
                     /locus_tag="VQU28_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HET2476106.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLGIYDFHHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            complement(20840..21991)
                     /locus_tag="VQU28_000721"
     CDS             complement(20840..21991)
                     /locus_tag="VQU28_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HET2476107.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNITIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKEEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(22006..22737)
                     /locus_tag="VQU28_000722"
     CDS             complement(22006..22737)
                     /locus_tag="VQU28_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476108.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDEDSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            22966..23067
                     /locus_tag="VQU28_000723"
                     /pseudo
     CDS             22966..23067
                     /locus_tag="VQU28_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(23039..23815)
                     /locus_tag="VQU28_000724"
     CDS             complement(23039..23815)
                     /locus_tag="VQU28_000724"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HET2476109.1"
                     /translation="MPKTLTKKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFVYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(23808..25031)
                     /gene="trpB"
                     /locus_tag="VQU28_000725"
     CDS             complement(23808..25031)
                     /gene="trpB"
                     /locus_tag="VQU28_000725"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HET2476110.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGAEAAGFGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(25009..25608)
                     /locus_tag="VQU28_000726"
     CDS             complement(25009..25608)
                     /locus_tag="VQU28_000726"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HET2476111.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVAADLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQSFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            complement(25595..26362)
                     /gene="trpC"
                     /locus_tag="VQU28_000727"
     CDS             complement(25595..26362)
                     /gene="trpC"
                     /locus_tag="VQU28_000727"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HET2476112.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDC
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNTGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(26359..27363)
                     /gene="trpD"
                     /locus_tag="VQU28_000728"
     CDS             complement(26359..27363)
                     /gene="trpD"
                     /locus_tag="VQU28_000728"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HET2476113.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEDYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(27374..27940)
                     /locus_tag="VQU28_000729"
     CDS             complement(27374..27940)
                     /locus_tag="VQU28_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HET2476114.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(27937..29298)
                     /gene="trpE"
                     /locus_tag="VQU28_000730"
     CDS             complement(27937..29298)
                     /gene="trpE"
                     /locus_tag="VQU28_000730"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HET2476115.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQAIDRDPLDFLYEVTHKSQHHSDLPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRSVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            complement(29799..29876)
                     /locus_tag="VQU28_000731"
                     /pseudo
     CDS             complement(29799..29876)
                     /locus_tag="VQU28_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(30481..31482)
                     /locus_tag="VQU28_000732"
     CDS             complement(30481..31482)
                     /locus_tag="VQU28_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2476116.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(31590..31958)
                     /locus_tag="VQU28_000733"
     CDS             complement(31590..31958)
                     /locus_tag="VQU28_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476117.1"
                     /translation="MKDYKINFDLGKIEYFDNNCLIQVYKFISFYDICEMVFAFHLPP
                     DELITNVIFKEKINSMLKCYIDRLLYVFINPTHFTEKVNLQFYGSFFSYEFICREVGN
                     ILKNKGVKCNLNFFEGEEYL"
     gene            complement(32054..32764)
                     /locus_tag="VQU28_000734"
     CDS             complement(32054..32764)
                     /locus_tag="VQU28_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HET2476118.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
     gene            complement(32767..34458)
                     /locus_tag="VQU28_000735"
     CDS             complement(32767..34458)
                     /locus_tag="VQU28_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2476119.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            complement(34470..35480)
                     /locus_tag="VQU28_000736"
     CDS             complement(34470..35480)
                     /locus_tag="VQU28_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476120.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            complement(35583..36650)
                     /locus_tag="VQU28_000737"
     CDS             complement(35583..36650)
                     /locus_tag="VQU28_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2476121.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            complement(36675..37334)
                     /locus_tag="VQU28_000738"
     CDS             complement(36675..37334)
                     /locus_tag="VQU28_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HET2476122.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
BASE COUNT        12046 a         7633 c         5730 g        12043 t
ORIGIN      
        1 gtacaccata gtaattgttg agtttaatgg gccaccttgc gtcataacca ttggttgtac
       61 aatcagctta aaagcagaaa ttaaagttgt taacaataca aacagggctg ttggttttag
      121 taggggcatc gtgatataac gaaactgagc ccattttgag aatccatcta attcagcagc
      181 ttcatagaca tcttgtggaa tattctgcat tcctcctaaa aataaaagca tctgataacc
      241 tgcaccctgc caagcagaaa caaaaacaat tgcatacata gcttgtttag gactagtcaa
      301 gaaaggttga gaagcaatac ctaccttatt taaaatagca tttaataagc cactatttgg
      361 atttaaaaga taaagccaaa gaatagaaat aactaccaag gacatgacaa ctggagcaaa
      421 aaaagccact ttaaaaaaca tatttccttt tcgcttttta ttgacaatca aagccattcc
      481 taaggccgct cccaattgaa ctggaataat ccaaacaaca aatttcaaag tattcaagaa
      541 gctctttaag aaaatcggat cttttgttaa tcgaataaaa ttttcaagtc caacaaattt
      601 tctttcatct ggtgtcaaca aataataatc tgtaaaagcg taataaatga ccattccaac
      661 tggaattact aaaaagatac ctaataaaat taaagctgga gctaaaaaac tataacctaa
      721 tatgttatcc tttatttttt cacgtctttt tgtatacaca acagtttctt tactcatatc
      781 catctccttt aatcatatat atcaaactag gctattaaca agaaagggga aggtagttcc
      841 aaacattttc cttccacctt cttttttaca gattatttga gtgactgatc aatagcagtt
      901 tgcatttctt ttgtacgagt atccaagact ttttgcacat ttggattttc ttcataatag
      961 ctaatatctt gcattgcttg ttgaaaagct cgagaaacct gaggatatgc tacgacaact
     1021 gggcgagcat gagctgtttt tgaattttgt tccatcaaga aacgcattgg ttcggatact
     1081 ttatctttga tattttcaat tgtagattta cgaattggaa ggaccgaatt ccccaagcta
     1141 ataatttcac ttgattctgt atttgtagca aatttaacaa attcagatgc ggcctctttt
     1201 ttatcagatt ttgtcgtcac agctaattgc caactacctg aaggggatac taattttttc
     1261 gtcttactag atactgggta aggaaggata ccaaaatcaa tatctttgta gtttgtattc
     1321 atgtcagcaa tcgtccaaga accactcaaa agcattggat actctcctgt ttcaaaacct
     1381 ttttcgactg gactaacagt tgtatagcct tcttttacaa gattttgaat gaattgaact
     1441 gcttctacac tctctttgga gttgaagtat ccttctgcct ttgttccatc ttcgtttacg
     1501 acagaaccat tatttgacca aatcaacggc atataagcat atggcaacat ttcatcgttt
     1561 gaattaatac gaaaatcaat tgctggtttg ttataatgat ctttcaattt tttagcaatc
     1621 gtattaaatt cgtcccatgt ccatggcttt tctagcgttg gaagctctga ttccgcaatt
     1681 ccagcctctt tgaacatttt tttattgtaa taaacaccaa cattcgattc agaaaatcca
     1741 aatgcataga atttcccatt ataagttccc tgttgtttaa tactgtccaa aacatcatcc
     1801 atattattat cttttagata atcatctagc ggagtaatta ctttagattt tgcataagct
     1861 gccgtatttg gtccatcaag ggtaataaca tctggaagac tattagtcgt aatagcagca
     1921 ttaaccttgt cttcatatcc tccaccacta ccactacgag gaatatactc agttacaacc
     1981 ttgtacttac tttttcctga ttgattaaag ttatcaacaa ctttttgcca agcttttcct
     2041 tcatttgttt cgtcagagaa ttgtacccag actttaatat catttgaaga actcgcttgt
     2101 tttgagttag ttcctccact acaacctgca agtatagcta acgatagccc cgcaacaaca
     2161 gataacattg tacctttacg catcattttt ctcctttttg aaacgtttca ttttttattt
     2221 attttttaaa caagcttcta gcctattttt gaaacgtttc atttgttgat tatattataa
     2281 cagcgctttc attaattgtc aagcgctttt taaatttttt ttgttgtttt atctccccca
     2341 aaatatgtgg gaattacgat tcgtttttct agaatctctt ctcgcttaat cattttcagt
     2401 atcattgcaa cagattcttt tgccatcata ccaatatctt gaactattgt agacactaga
     2461 taagattgat cgacagaagt ttttaaacca tcaaacccaa ttatctggta atcatcaggt
     2521 gctattttcc ccagttcttt cataacacta ataactctca gagccatcat atcatttatt
     2581 gtaaatatac catcaatttc tttatgagat tgcaaaaaaa ttcttatctg atcttcagga
     2641 tttattaaag gttctttcat ttcaaaatta tagacatcta ctcctaattc cttagctttt
     2701 tctctgaatc ctttacttct gagtatagtc tcattctcat acatattaat tgaaccaata
     2761 aaagcaaggt gcttattatc ccttctaacc agttcctctg ctgcagtttg tcctcctttg
     2821 taatttgctg aagtaacata gcatacattt tccgaaaaat gtctatctat acttacaaac
     2881 ggtaaatcag ataaaatata tttgtcaata tcagaatatg taataccaat aattccatcc
     2941 actttatttt gtttcaacat ttcgatatat tcgatttcat tttgtgaatc gctatcagcg
     3001 ttacaaataa ataatttata attatatttt aataactctt tctcaacatg ataggcaaac
     3061 tccgaaaaaa atggatgcca tatgctcgga ataatcaaag cgatagtttc tgttctattc
     3121 tttttcatac ctcgagcata attatcaggg atatagtcca atgtttttat agccctttga
     3181 actttcctta aagttacttc tttaatgcct ttttctttat taattacacg tgaaacagtt
     3241 ccaacactaa ctcctgcttc taaagcaaca tctttcatgg taattgattt tctttgttct
     3301 accatattat cacctccttt caatatatag tatcatgcaa atgcttttta agcaactatt
     3361 tctcaatcat ttttggccag atcatttatc ccatcatgaa taaaatcact ccaattagct
     3421 tttgaaaata cttcaatttt catgtgtaaa catctacata aaacaggaaa agccttggtt
     3481 ttatggcttt tttcgtatct tctacaaaaa aagcaagagt tttagagggc tataaatcta
     3541 gatgtacatt ttgcttaaat gattgaaggt cttttcttaa caaaaacacc ccaaaaatta
     3601 gattttttct gtctaacttt tgaggtacag ttcaaacgcg aaatagcgtt tttttgttat
     3661 ttttggttac tcatctaatc gaataaacat catggcattt aacaaatata tgagtgagac
     3721 cgtgtttata ttatttgaat agatgagtct cttattttca ataggaggaa taataaaatt
     3781 agaaataatg atatcataag atgaatcttc taaagattcc tttgataatt ctaattcagt
     3841 ccaaacttcc agttcaaaat tattgctaca gtaataagaa agtgtctctg caacgaattt
     3901 tgcatgatac tgatcaaaat tactcataac caaaaccttt agtttaggct gattttgtag
     3961 caaattaatc accaaatgtt tggtatgagt gatgaaggta taagatagat gatttaccat
     4021 cattgaacta gaacaaacct caagagtctc taaatagtga gaaagctctt tttttatatc
     4081 tgaaacaaat tttggaaaaa tattttgaaa gttcctgatt gtattccctt tttgatcaaa
     4141 taaaataaac tcagtaaaca actcttgacg atacagatgt gcggtattat gcagatgcca
     4201 aatcagatta tccttattct ccatttcaat ctgatacttg actgaaatct gatcaataaa
     4261 atcactcaat agatggtaag atttttcaac ataactatcc ttttttacgc atttcataaa
     4321 gagactttca tctatgaaaa acattttttg aaagtaagac acaaataatt ggcaaacaac
     4381 ttcttcatct aaagagatat tgtattctga ttcaaaactc tgagcaacac cttctattcc
     4441 ttctgcctgc attaaaaaat ccaaactttg gtcgttaaaa gaatctttat ctacttccat
     4501 aaaatgacca aactttattc tatataggtt cgtaactagg agcaacttta gcattctatg
     4561 cgttgacaaa ttcattggaa agcttgtttc cttataaacc aattctaaca attgagatag
     4621 tggctctgat gaaaaatttt caaatggcca ttctaggaaa taatattttt ctgaaaaata
     4681 ttgtgcaaaa aagtaacgaa tgtctctctc atttccaatg atttgaacag gggtcagact
     4741 gacttcaaat tgaaattgcc ttttaatcac tttattgatt tggctaataa tacgatagag
     4801 cgaagatgaa ctgatataaa attctttaca aatactctca gcttgacaac cttcattaaa
     4861 gaagatgaat tctaaaatcg aaaaatgagt tgaatgttta aagaaatgat ggtaaaccat
     4921 ttcaatatca ctatcatcgg tattaataat gcgtatacca ttagtagaag aatgaaaaat
     4981 caagtcagga aaagcagatt taacatggga tagatcatct ttgactgcac gttctgtaca
     5041 atttaataac tctgctagtt cagaacgatg aaaccaacgt ttatgttcaa ataataattc
     5101 taataattct aattgcctat gacttttttt agataataaa tctctcatga atatctttct
     5161 ctctttataa attatcggat taaacctctt gcaattatac cacaaagaat aggtatagca
     5221 tgatataacg acttttccta aaatctttta tttcgtataa taacactacg gagacaatat
     5281 ataaacaatt ttcttatttt accgtctatt gagggcgtga atacagaatc aaattcaagt
     5341 ctaaagatta tatttttaat tttaaaaatt atataatagc aacaattaaa gaatttgatt
     5401 ttttaaaatt atataataat aacaatcgaa ataattgact tttctatatt aaagttatat
     5461 aatagtatta atcaaagaaa ttgatttttt gatattaaaa taaaaaagga gggtaggcag
     5521 tgttgtgatc aattattgct ggaggtctta ttggtctctt ggcaggtaaa atcactaaaa
     5581 aagtagttct atgggaatca tcgcaaatgt attcgctggt ttagtcgggg catatgcagg
     5641 acaatctctt ttaggtagtt ggggtccatc aatcgctgga atggctttgc tcccatctat
     5701 tgtaggtgca gcgattgtga ttactgtagt gtcattcttt acaggtagaa agtaaacttt
     5761 tcgccagtaa agttagcaaa ctatttttaa atcaatgacg ggaaaaatag tttaaatgtt
     5821 aaatcgaaag gattgtatat gtcaaaagca aagaaaatat gtttcattat tttctgtatt
     5881 ttaatcttga caattttcct tcctgttttg atagattatc atcaagttag tgatctaggt
     5941 attcatctac ttagctggag acagaactcc gtagttgaat tctatcttgc tagatatgtc
     6001 ttttggggga cagtggttct atcaacttta gttttattat ccattttagt tgtgatgttt
     6061 tatcctaaac gttacttgga aatccaactt gaaactaaaa acgatacatt aaaattaaag
     6121 aattcggcaa tcgaaggttt tgttagaagt ttggtgagtg atcatagatt gatcaagaac
     6181 ccaactgttc atgtaaattt acgaaaaaat aaatgtttcg ttcatgtaga aggtaaaatt
     6241 cttccttcag acaacatcgc tgacagatgc caaataattc aaaatgaaat aactaatgga
     6301 ttgaagcagt tttttggtat tgagcgtcaa gtaaaacttg aagttgcagt aaaaaattac
     6361 caaccaaaac ctcaaaacaa aaagactgtt agtcgtgtga agtaaggaag taaaaaatgg
     6421 aatggcttaa acaatatcga tatccaatta tcgctggtct cataggcgta tttctggctt
     6481 gtttgattgt ctcctttggc ttcttcaaaa caatatttgt attgatttta ggagcactgg
     6541 gagttgcagc tggattatat atcgaaaaaa actatataga taaataaaaa aataaaaatt
     6601 actaatttaa ttaaaggagt ttcatatgtc aaacgaaaaa aacacaaata ctaacgtaga
     6661 aaagaaagat gctactgttg tagctcacga aatcaaaggg gaacttactt acgaagataa
     6721 agttatccaa aaaatcattg gtctttcact agaaaacgtt tcaggtcttt tgggaatcga
     6781 tggtggtttc ttctcaaatc ttaaagaaaa aatcgttaac agcgatgacg taacaagtgg
     6841 tgttaacgta gaagttggta aaacacaagt tgcagttgac ttaaacgtta ttgttgagta
     6901 ccaaaaaaat gttccagctt tatattcaga aatcagagaa atcgtatctt cagaagttgc
     6961 taaaatgact gacttggaaa ttgttgaaat caacgtaaac gttgtcgaca tcaaaactaa
     7021 agaacagcat gaagcagact cagtaagcct tcaagatcgc gtatctgacg ttgctgaatc
     7081 aacaggagaa ttcacttcag aacaattcga aaaagctaaa tctggtcttg gatctggttt
     7141 ctcaactgtt caagaaaaag ttagcgaagg tgtagaagct gttaaaggtg cagcaaatgg
     7201 tgtagtatct cacgaaaaca ctcgtgtaaa ctaagataaa ataaatataa caggagaaat
     7261 tatcatgtca gtagaagaaa aattaaatca agctaaaggt tctattaaag aaggtgttgg
     7321 gaaagccatc ggtgatgaaa aaatggaaaa agaaggagca gctgaaaaag ttgtttctaa
     7381 agtaaaagaa gttgccgaag acgctaaaga cgctgtagaa ggtgctgtag aaggtgttaa
     7441 aaacatgttg agtggcgatg ataaataagg ttaaaagtta ctttatcttt ttggtaacat
     7501 tagtcaaaag agtctgagtc aagatgattc tcagaaaaca aaaagctaga gattcccaat
     7561 tgcggaactc tagcttttta attttgcctc tttctcttat tatatttcag caggttgttg
     7621 gccatgagta tgaatcccat gtcaattctc acttgacgct tacctctcag atgacatctc
     7681 ttataaccca aacaaacctt tatctgccca aagacagatt tcatatcaat cttacgttta
     7741 gcgaaaattt gtctaccctt ggaagataaa agtgcctgat attctttagt ttttaaacac
     7801 tggtaacgtt cattcatata cagtcccttt tgaggggctg attcaggttc ataatcgcag
     7861 tcaacattga tttcaaggct gtttgctttc tatctcccct ttttcatgat atattttcgt
     7921 acaggttctt caatagtttc tatagtaaaa tgaaccaaaa atagtacacg ttgcggtata
     7981 atgttcttgt ggcatattca ttagattttc gtaaacagtt ctctcctatt tgtgagcgaa
     8041 caagtatagt caattgattg tcagtttgaa gaccaagagt cgtaaggcaa aggatatggc
     8101 agaatccatc caagggtggc tggcacaatt tctagtgaac ctctttaaat ctattacgtt
     8161 tgactgtgga aaagagtttt caaaatggaa agatatttcc aatcatcacg atagtgagag
     8221 cttttttgcg aatctaggat gttcaagaca gcggcgtctc aacgaacatt caaatagact
     8281 tttaaggtgt catgacttgc caaagcagac agatttcaac gaagtcagtc aagaatttta
     8341 ggctggacaa aaactcaatt tcaggagaaa atgaaataaa tattcccaca ataaaacgca
     8401 tagtatcaaa gtttttccaa cacctgatat tatgcatttt ttaattttaa agactttttc
     8461 ccagcctcaa gaaatcacat cccacctaaa tcctttggat accaaatacc tcatcaggta
     8521 ttttctgagt tggtaagaat gtctagctta atttgacaaa tggagggcgt taaatccagt
     8581 ttttacagga agtctattta aatattttca cactgacctt acaaaatatg aaataaatta
     8641 tataaagtca ttcccatgac aaagaataac atccctttaa aacagtatct tacccaattt
     8701 tcaggtaaga cttttgatac aaccgtcccc aatacccctc cgcatataga agcaagaaaa
     8761 ataactggaa caggtgctaa atgatactga tttaaacctg taaccacaac aattgtagcc
     8821 aaatgagaca gctgggaaaa gaaaataatt gcaagagaat acatcgttgc ttcttttatc
     8881 gaaatagaaa aaaacaccat caataaagat acattgagcg gaccaccacc aatccctaaa
     8941 aaactagaaa ttaagcccaa tagaagacca caaatcatat agaatcctat cttatcaaat
     9001 tgataagttt ttttaaaatc tgttaaaaca aatgatacaa gtagcatcac aaatagtaaa
     9061 atcatttgaa caatggatac tagccgtact gataactgag ataacaaaac ttgaaaaata
     9121 agatgaccta gaaatcctcc taaaacagaa gaaaaggata ccgtaaaaat caatctccat
     9181 tttatctctt tactttgtga aaatcttttg attgttgaaa taatcgccat tataaaaaca
     9241 gagaaactag aataaaaagc aatatcactg accgaatggt atccaaaact atccataagt
     9301 ggcttaatta taattccacc acccatgcct gatatagaac ccaatccact agctattata
     9361 atgattagaa aataaataat ctcaaccact ttttaagtcc atcctattct ttcaaacatc
     9421 taaaacctag atggctacta gtggaagtag agatacatcc atttctagca gctactcggt
     9481 atcgattaca ataagaacaa tgacataaaa aggaacctcc tctgatagca tactcctcat
     9541 cttgtatccc atattttggc aactcatagt catcttcatt aaaactagct aataaggtat
     9601 atctcggatt gcgacaccat tcccaaacat tcccaatcat ttgatataga ccaaaatcat
     9661 taggtaaaaa ttcatagaca ggagccgttc ctataaatct atctaaggca gtattttcat
     9721 aaggaaatct tccttgccaa gtattggcat gataatatcc accttctaaa agagtatctc
     9781 cccaaggata ttcagattgt cttcctcctc tagcagcata ttcccactgt gcttcagtag
     9841 gtaaagacat tccgctccaa ttacagaaag ccaatgcatc ttctaaagca acatgaacta
     9901 ctggatggtc ttctaaaccc actagattac tgttctcacc gtagggatgt ttccagcaag
     9961 cgttcggaac ttgtaaccac cacggagccc cagcaggatg aggatatccc tccctttctg
    10021 cctctggaac gaacaatata aaaacaaaag accactcttg tcgctcagct agtgttttat
    10081 agcctgtttc tttcacaaat tgagcaaatt cttgattcgt aactggagta tccgcaatgg
    10141 aaaaatcctt tacactaaca gcgacttgag gaccttcaaa atcatctaaa aagccctctt
    10201 cagaatttgt tcccatcata aatgtcccac ccttgatttt tttcattttc atgattgact
    10261 atcccccgtt ttcgttagag ttggcgttat tttagaaagt tcaacaggaa ctaactcttc
    10321 atccacaaca aaaccttctt ctctatatct aagaaagtct actaattttt tcttcatctg
    10381 acgaacaata ggttggtatt tttcgcttgg atacaaatca ttcatttcat gcggatcttt
    10441 tttcatatca aataactgat aatgatttaa aacaggaaac cagataaatt tccactggtc
    10501 tgtcaagata tattgactag aatcttttcc taaagcatgt tcaccatgaa attctgttcg
    10561 ccacccctcg tactgtccaa ataataaatt cttaacactc cgtccatcta gttcatctgt
    10621 agtagttcct cctgccaaat ccacgagtga aggaaaaata tcttgaattt tcactaattg
    10681 cttgattgtt cccctattac cagctatcaa tccagccgga tcgtaaataa acgaaggaat
    10741 gtggatactt ccctgataag gatatccctt tctaaacaga taatgttcac caagctgatc
    10801 tccatgatcc gaaacaaacc agataatcgt atctttatca tgacgaaatt cttttaggga
    10861 agtcaaaaag cgactaattt gatggtctat atgagtaatc aatccaaaat aagcagcaac
    10921 cattcttctt tgatcatctt cctttaattt ccctcttaaa gcataaatag acggaatttg
    10981 cttttctaat acttcccaat tcccgatatg caaatcgaga aattgaggca agcgttccat
    11041 atacatatca aaatagtatt ttgggggatt taatggagca tgtggttttt caaaagacat
    11101 tttgagaaaa aaaggaaccg ttggatctct tctttgtaaa aatgaaatgg actcactcac
    11161 aacccagttt gttggatgta acttctcatc tttatcccaa ggtctagctt cccaagaatt
    11221 acaatccatt ccgtcatcaa tcaaatcaac atcataacca actttaccct ttaaaaaggc
    11281 tagatagtca ctaacataat caaattgact tccataagcc ttatcatact ttctatcaac
    11341 atgtagatac ccatcgtgaa gtaagacatg atcaaaccca agtctctgtc tggaaggaaa
    11401 aacatgcatc ttaccaatac actctgtttg ataacccata tctttaaaga cttttggaag
    11461 agtattagta aaattccaag gcacctcatc ttgataacca acacgtccac ttttatcttg
    11521 gtctaagcca gtcaataaag ctgccctagc aggaacgcaa gaaggaactg gagaataggc
    11581 attttcaaaa ttataaccta cactagccat catatctaat gttggagtgc taactagctt
    11641 atccttacta ttcaaggata aggcgtctgc tctcatttga tctacaacaa tcaaaataat
    11701 atttggctgt tttgtctgaa ccataaaatc tcctttctaa tatggcaaaa gaggcacaag
    11761 aagatatcta cctttactgc acccctttct atatcaatct ctctatataa agcaataaca
    11821 ttcttgttat gttttataga acaatgaact aaaatatgac taaatcgatt aggaaattca
    11881 aatcattttc tagtactgtt ttagaagtta cagtgtacta ttccaacttc aacaaactat
    11941 aaaccttggt ctaataacaa ttttagtgga gataagaaat cctacaccta actcatctta
    12001 cacgtaatct atttctattt tatcacaaaa aacgcaagta agaccattaa ctcaattcag
    12061 ttttatctgc cattttcaca aatgggaaat aagtcaagac actaataatc aaacaaacaa
    12121 ctgataagat gatggcacgc caatcaaatg ctgtagagaa gaaaccatat aaaattggag
    12181 gcattaccca agtaacattt tgtgtaacag gtgaaacaag accccagctt gttgcccagt
    12241 aagctaccgt tgccatgaaa accgggctaa gtacaaatgg tataaatagc aaaggattca
    12301 agacaactgg taaaccataa ttgaccggct caccaatatt aaacagaact ggtgctagac
    12361 caagtttagc aacttttcga taatgactgt ttcttgaaaa aattaaaata gcaagtacca
    12421 atcctaatcc tccaaaccag acaaacgccc caaaagaccc acttgtccat atataaggaa
    12481 tcggttcacc tttttggaaa gcatccagat tcgctaacat agcaactcca aacagccctt
    12541 ccatgatggg agccaataca tttcctccat ggagaccaaa aaaccagaat aacttattta
    12601 aaaagatcat cagaataact gcaaagaaac tttgagacaa acctagtaat ggcgtttgta
    12661 acaccttgta aacccaatca atcaataagt cattgctaag taaatggaaa acataagtca
    12721 agatggctac tatatacatc gccataaatc ctggaatgat agaagtgaac ggcttagcaa
    12781 tcgcaggggg aactgaatct ggtaacttga ttacccagtt ctttttcatt actttacaga
    12841 aaataataga ggctaaaaat ccaatcatca tggctgtaaa gtagcctctg gcattaatat
    12901 ggtttcctgg aatcacattc ccaatagtta ccatcagatt tttaccatca aatgctagat
    12961 tatcaattcc ttgtaagatc tgatctaatt tcacatctcc tacatttgcc aaagggaaac
    13021 tctttgtaac tgtacttcca atcgaaatga caaacgaagc aagtgatacc aaaccagcag
    13081 aaactgtatc aaccttgtaa atcttagcga tattcactcc caagcaatag atgaacaaca
    13141 aagaaacaat tggtatactt cccttgaata ccaaattatt gatgtcaaca agccactgaa
    13201 aggttttcgt aatactttct aggtgaaatt gttgtggtaa atccactaga aaagcattta
    13261 ataacaaagc aatggaacct gtcataataa caggcatagt ccccacaaat gaatcacgta
    13321 aagcaactaa aaacttctga ttcccaactt tcattgcaat tggagaaagt ttttcttgca
    13381 ttttatccaa cattgtattc atactaaact cctaattttt ttcaaatcgt tcgtatactt
    13441 ctaccacttc tttcgccaag tcaacaaaag tcagactcgt cattaaatga tcttgcgcgt
    13501 gtaccatcaa gaggctaatt gatgtttctt ccccctgtgc ctctcttgtc aacatttctg
    13561 tttgagcctt atgcgcctgt aataaggcag cattcgcatc agctaatctt cgattggctt
    13621 tagagaaatc cccttttttt gctgcctgaa tagcctccat agcattactt ttggcttccc
    13681 caccatacat aattagcccc attacagatt ctaaattact ttcatccata acattctcct
    13741 caatcttagt ctaataaact ttcagctaaa tctaaaactt tagaaccatt catcattccg
    13801 taatctgtca tcggaataac cgccacagga atctgtctat cttttagttt ttcttgaaag
    13861 tcccctagta aatagcgaac ttgggggcct aaaagcaaca catttacttc ttttgttgct
    13921 acaatttctt ctgcttcagg agctggaact gcaaaaattt ctgcatccaa ccccttatct
    13981 tctgctgcct tttgcatctt tgtcactagc agacttgtac tcatacctgc tgcacacgct
    14041 aacataattg ttactttagc catagcttac tccttttatt ttattgtcaa tgttactgtt
    14101 tttcgatcaa ctttagactc ataatctaaa tgtttgggac gactagtata atgcaaatta
    14161 taaactcctg gaggcatttg tgaaatcttt tctttttcag ttttattgcg aattaataaa
    14221 tctaccgaag tattccctcc aaagttatta aacataggtt cattttcaat agaataatct
    14281 aactcatatc cactattagt tgtaattcct tttaataaat caggaacatc tcctctgttt
    14341 attactatat ccttagcgcc atttattttg ggagattgac tataatactc ttcagcttga
    14401 tgttgttgta aatcatttct agagtaagaa acataatcaa ctacaaattc agtccctgat
    14461 aaattattgt ccactacccc aggccaactt cctcccatcg ctaaatttaa ctgaacatac
    14521 tgaggtctat taaaaatctt tttcgctata ttatctgatc cataatatac ttttctaaca
    14581 atatgattgt caaccatcca tacaatataa tctttatacc aatcaattga aaaagtatga
    14641 tacgaatcat taaagtttcc actcgataat ttaaaggctg taccacgtcc tgcaaatttt
    14701 ccatcatcat tatccgactg tccataatga agcgtttgat aaactgtctt attcccactt
    14761 ccatttgaat ttcgttcacc aactaattcc attatatcta tctcacctgt tgacggccat
    14821 cctcgtccct gaacaggatt aatcttccca tcaagtgtaa aatccgatcc aagagtccag
    14881 aaagcaggaa aaactccttg acctttcggt aattttgcac gcatctctaa tttaccataa
    14941 agaaattcta ctttcccatg tgttctaaca cttcctgaat tatatacaac ctttctgtga
    15001 ttgtttcttg ggttaaaata ctgatcttcc ttagagcgat tcgtagctct aagaactaaa
    15061 tttcccgaac gtagaaaaac attctcatca cttcttacat aatgttgctg ttcccaacca
    15121 cgaattgatc ctaattcata tccccaatat tttttattta attgttcgcc atcaaaatca
    15181 tctctccaaa ttttcttata tgaatttcct ttagacagtt gatacatctc tacattatca
    15241 ataaaaatat tagatttttt tgtagtatcg tcttctgacc atttcactaa ttgaatcaaa
    15301 actttatcgt gtttaccgga attgaaagaa aacatgtatt ttttccattc ggttgtatta
    15361 actgtttgat caacagcttc tccaatgtta ttttgaacaa tctctccttg cttattatta
    15421 tcaaaccatc ttgcggtaaa atatccctct gctccactaa tatctacttt ggcaaaagca
    15481 gatactaaat aatcttcatt aggttctata tcaataactt gtccaatata tccatctgct
    15541 ctattatcat taattgcagt agaaggaatc atacctgagt aatgtccgtt actataactc
    15601 tcatttgaat taattattcc cctatttcta gagttccaaa acagctctcc ttgctcaaaa
    15661 tttgaatttt ttattaaatt ttcattagca aaaactttca catgaataaa tattaataaa
    15721 aataatataa ataaacttga aattaatttc ttattcttca ttttatcacc tatttactga
    15781 cctagccaag cgagatactg tatgtctcac tcctctaatt gccttgctca atgcataaat
    15841 attatcgact gttgccagtc ccccatatcc tgcgtcacct atatgttgaa tatcaactcc
    15901 acaaatttta tttctaagtg caatttcctt gatagtatct gtatcagatg tttcttggct
    15961 agtaccaata gcactcagta ctagcccacc cttactatga acgagatcaa cgacttcacg
    16021 caactcttgg tcatgaaagg ctggaacggt tccaactgct ggaacaagta tcacatccgc
    16081 acctgcttcc aacaattgct ctgcgacaga aagctctgcc acaggctcat tcactcctgc
    16141 cccgtgcatc ttacctgcaa taatcaaacc agaaaagttt tccttagcag tttgaacagc
    16201 cttaatgatt tctcgattgc taactcctgt tccaggattt ccagtcaaac agacaaagtc
    16261 aaaacctaac tcctctattc gattcaatgt ttcaacacta gcaacacgac ctgcaacaat
    16321 ttcctgtgtt tcctctagca tctttgcaga tgggtcaatc ggttccaaat ttacaccaat
    16381 tggacaagca acaagctggt gtaagcgatg aataacatca tcacctgaac tatccaaagc
    16441 ataaatttta ggctcaaaaa catcaacaca attcaataaa atcatatcag ctccaaaagc
    16501 tctagcaatt tcagaattag ttatatcccc aacaaatgtc tcacgagtaa ctacattttc
    16561 agatagaaca actctacctt cacttgctaa aatactttgt tttaattctt cagcattcat
    16621 ctgtaatatc tcagatggat ttgcactaat taaacgtttc attctcaatc ctccaataac
    16681 ttaaaccgtt tttcaatagc tattacaaat ggtaaataaa gtaagacatc caaaacaatc
    16741 aatacaattt gtaataatga accagaaata cttcctgtag caagtaaacc acttataaca
    16801 ggtggcatag tccaacttgg atctgtataa gttagcggaa ccaaacctga tgccattgct
    16861 ccccacgcta ctagtaagtt aaacattgga gctaagataa atggaactag taacaaaatg
    16921 ttcaaaacaa taggaacgcc aaacattgct ggttcattta tattaaataa tccaggtata
    16981 acagttatag gtgccaaagt ttttagttgt ttactagctt tcttcttatg tgctaaatat
    17041 cctaacgcaa gaactaaacc tatggttgca ccaccgccac ctatgaatac aaaattatcc
    17101 ataaatacgc tagtaaaaat atttggaaga gtttctccag tttgataagc tactttattt
    17161 gcatctagat tagctaacca taacggtttc ataactgcat taacaacatt tcctccgtgt
    17221 aatcctacaa accaaaaact actattaagg aaaattaaga taagaagacc aataacatta
    17281 tttcctaaaa gtccaagtgg acctcccaaa gctccttgag caatttcatg aagattaggt
    17341 aaacttaatt tatctaaagt tgcataaaca atcaaccaac caacaatagt cactgcacca
    17401 ggaataatgg cactaaagct tttagatact gctggtggaa caccatccgg catttttatc
    17461 tgtatattat gatttataaa ccattggtag atatagccat taatcaatcc taaaatcata
    17521 gcaacaaata aaccttttga tgccatgaaa gcggtaggca ttcctgctcc agcctctcct
    17581 gtaatgaaag gcgtaactgt aataaaagat gataatgaca caataccagc aggaatacca
    17641 tcaactttgt attgtctagc cataaagtaa gcgataccaa aactagctac aagaccaagt
    17701 aatccaaaac tactatcaac acccttccat aaatagtcag caacaccaat atcacctaga
    17761 tatttttccc atcctggaat aggaaaactt gcaataacca ttagtaatga acctatcaac
    17821 aataaaggta tagttagagt aataccgtct cgaatagaaa ccaacgcttt attattaccc
    17881 aatctagccg ctactggaag taacttttct tgtaataatt tttccataac cttttcctct
    17941 taaatatatt ttattatcaa aagttatcaa ttatctttat tttttgacgt caagttatta
    18001 tttgattatc ttttgttatt tatattttac aactataaga aagccttgtc aaggatttgt
    18061 gtaaatattt gattattttt tattatcaat atataataaa aagagaccag taagcaatga
    18121 aaagcttaaa aagattaaca tctacattta tccatagcaa aaaatcatag gattttaaga
    18181 atctgacaac taggaagaaa actaatatga ccttatctaa gaaacaatta caactaagag
    18241 ctaaaatact ggaaacagtt tacacattag gtcccatttc acgaattgaa atcgctacta
    18301 aaacaggaat aacacctgca acaactagca gtatcacaaa tgatctgatt aaagaaaata
    18361 ttctcttaga acttggtgaa gacgaacacg atacaagcgt agggagaaaa aaaatcctgc
    18421 tagacattca agctaaacga ttttactaca ttggttgtga actatctgaa aaacatttca
    18481 cttttgcttt aggagataat ctaggaaata tcttaaagga agagaaagaa attgttacta
    18541 aacagttaat ccaagaaaag ggaaatcaac tcattaacca gacactaaag caattcttaa
    18601 ataactgctc tgattatgaa atagaagcaa ttggaatcgc gctacctggt cgctatttag
    18661 atgattataa aataacaaca aacaatcctt tatggcaaca tatcgactta gaaatgattc
    18721 aatcccactt tgataagcct ttgtttttct ctaacaatgt taactgtatg gctataggta
    18781 aacgcctatt tagtcgccaa cagaatgacc ctaattttgc ttattttcat tttgctagag
    18841 gtatgcactg ttcttatatc tacgacggaa acatttatgg gaaaggaaat ctaatgattg
    18901 gagaaatagg gcatacagtt gtttcatctg agggagaaga atgtagctgc gggcgcaagg
    18961 gatgcttaca aacttttgct ggagaatctt ggttaatcaa aaaatcaaaa attctatatc
    19021 atcaatctcc ctattctcta cttcctagtt tagtcaaaaa tgccgatgat attgatatcc
    19081 aagttatttt aaccgcttat caattaggtg acactggaat aatcacactt atccatcaag
    19141 ccttactcta tcttagtcaa accattttaa atatcagtat gatgattgac tctcaaaaaa
    19201 tctacctaca tagtccttta ctaacaaatc aacacatcat ccaaaaacta tatagtgaaa
    19261 tgaactacaa acccaaactg ctctataatc gcctacctga agtcattatt gaaccttaca
    19321 atgattttac agccgcacac tcagctattg cactttgttt gtatcataca attctacatt
    19381 cttaattttc tattacattt aataaatagg ctaaaaagag ggagtatatc ataaaaagcg
    19441 aataaacctg ttttctgaaa atcagaattc tgttttgttc gctttcttta tatttggaat
    19501 tatactccta tctccccttt ttcataatgt atagtgaaat gaaataataa caggacgaat
    19561 cgatcgggac agtcaaatcg atttctaaca atgttttaga aatagaggtg tactattcta
    19621 gtttcaatcc actatatttt cgtacaggtg cttcaaccat ttgaacgatt tcaaatcctt
    19681 ctttttggta aagattctga gctctttgat ttgcctcgaa gacatttaga gaaatactat
    19741 ctatatcttc attttcaaag gccaaactaa caaatttcct taaagcctgg ctacctaagt
    19801 cttgctcctg tttctggggg ttgataaaaa atctcccgat atgaagattg ctgtcttcta
    19861 gcctgatttt ctggataagt cccacaaact cttgttcatc aaagattgaa aagactcctt
    19921 ccaaggcttg aaggggcagt agaaaaggaa tccttggtcc catccattgt tcttgaaagg
    19981 atttgcctag ggagttggac cactggcata caaattgagc gttttctata ctcacctttt
    20041 cttcaaaacg aattgtcatc ttttcctcac catcttatct atgtttctcc attatactat
    20101 ttctcccatt ttttacgaat agataagtat gattgatttt tatttttttc tcgtcgggag
    20161 cattctcgct tcctttcttg gtttggtcat tgaccgtttt ccagagcaat ccattatcag
    20221 ttcagccagt cactgcgatt cctgtcagac tcccttgcgt cccttagatt tgattccgat
    20281 tctctcacag gtcttcaatc gctttcgctg tcgctactgc aaagttcgct atcctgtctg
    20341 gtatgccctc tttgaactag gcttaggact cctctttctg ctttactctt gggaattgct
    20401 ttccttgggg caagtcgtcc taatcaccgc tggtttgacc ttgggtatat acgactttca
    20461 ccatcaggaa tatcccttac tggtctggat gactttccac ctaatcctaa tagcttcctc
    20521 tggctggaat ctggtcatgg tctccttcct catacttgga attttggctc attttattga
    20581 tatccgcatg ggtgcagggg atttcctctt tttagcttct tgtgctctcg tctttagcgt
    20641 aacggagtta ctgatcttga ttcagttcgc ttctgcgacg ggtatcctgg cctttctcct
    20701 gcaaaagaaa aaggaaagac ttcctttcgt gcctttcctc ttacttgcta cttgtgtgat
    20761 tatttttggt aagctactac ttgtttgata aattccaatt tctgccatat gtactccatg
    20821 aaattatttc acagttaaat tataaattat ttcttttgta caaagggatg atgttatcaa
    20881 atcgatctgt tcttctatct tcttgatact gatcaaaaaa tttcatttcg actgaaaata
    20941 tttcgcttat aaactgtaaa cgaatacttt gtttagacat tatagtcgct agactgacta
    21001 gatgattact caaaacgacg tccagaatac tctttacttt gcttggtttt ttaacaaaaa
    21061 tttgatcatc caaaggttca atcattttgt aacctttttg cgcaatttga cgataaaagt
    21121 aagaatgttg ctttggagtc aataatccta acttaaaagc tcgatactct aaagcctgta
    21181 tcgaaacatt caaatccgac ttcaataaaa tataactatc aggattgctg acacgcttgc
    21241 caaccctctc ttcaaatttg actaaaaact cttcttttgg caataaaaaa catgatgcaa
    21301 aataatttgc ttcttgctcc aaacgatcgc catcttcatt catatcttta tatttatgta
    21361 aaagaatatg tcctagctca tgagctaaat caaaatttcg acgtacagat gatttattcg
    21421 ttcccaacac aatataaggt cttcccaatt ttgaccatgc gctataagca tcagcttggc
    21481 cattaattaa tcgttccacg atatagatgc ctgaacgttc taatttataa agcaaatcat
    21541 gattatcttt tgaaatacct aatttttccc tggcataaag agccaattcc tcaatggatt
    21601 ctcccttatg ataagattca ctcactacat tacttaggtc atgaattgta atattaggta
    21661 taattacaaa actttcaaaa taatcaatca aactatctac cttatgtaaa tacatagttt
    21721 gaatatctat tgttttccgg gttgctaggt ctgcatttct aaaggcaatt acagaagaat
    21781 caaatcgaat gctctcttct tcctgttcaa aataagttaa atcaacatga aattggttgg
    21841 ccaaatgcat tttggttgat aatttaggtt tcgtttcgtt ggactcaaac tgccaaatgg
    21901 cttgttccgt taaattaatt ctctgagcta attctgctct acttaaacca tttaacagcc
    21961 gtaattcttt caatacccga ccattaaaca tttacatact ccttactact tttgaccttc
    22021 ttgtttttct attcttggaa taatttcaaa atcttctgtt tccgataatt ctgaaaaatt
    22081 aggaatatct tgatatttag cttcttcgaa atggtacgaa cttgattgaa tatactcaga
    22141 caaatcttga atcaagtgta actgaccagt tcgtgcatca ggcataacaa catctacaga
    22201 tataatattg ttttctgagt ccgcctcata agttaaaatc ataaattttt cgatattcga
    22261 atttttagta gcttgttcaa tttcttgaat catttcatca gaaactaact ccatctgaat
    22321 tggaaaggaa tgactatctt catcattttt gtaggaagaa tgttgattaa gataaagtgt
    22381 attcatctga gcatattcaa ataagtagcc actcttattt ttttgtacca aaggaaattg
    22441 gttcgtaagt cgcttcttac cctttataat taacaatact ttcccatatt tttctgtgtt
    22501 tgtttcaaat tctaaatatc cccaagtctg tcctgctaat tgtaatttat actcaaacaa
    22561 atctgctgat gcaaatgcag tatcaatatg attaggtcgc gtccatgcat aaccattcga
    22621 cactatcatt gtctctcttt tttctagacg ttcatctaca taatcttttt gccctttcat
    22681 caaagtatct acaatttttt gtgcctcaag cgaatcaaag agatcctgat tcaacataat
    22741 tcttcctcct ccaaatactt tttaatgaat tatatcattt tcttaaagaa attactacaa
    22801 taattagctt tttcttaaag ttctgtgtca gagtaattta gaaaattata tcttctatag
    22861 taaaatcaat taaaaactga acaaatttat tgggaaattc aaatcacttt ctaaaaatat
    22921 tttaggaacc gtagtgtaat attccagatt caatttacta taaaactagc ctttctcctg
    22981 caaaagaaaa aggaaagact tcctttcgtg cctttcctct tacttgctac ttgtttaatt
    23041 atttttggta agctactgct tgtttgataa agtcctgaat cggctctcct tggtggagag
    23101 cttttactat tttcgaaccg acgataacac catctgacac cgcattgaag cgttccagat
    23161 cggcttgact agatacacca aaacctgtca agactgggat gtcggccact tgatgaagtt
    23221 gcgccaagtg cttgtccaaa tctgcacggt aattgcctga tttccctgtt accccattga
    23281 tggcaacggc atagacgaat ccctccgccc cttcaatcaa ctctttctgg cgctcaattc
    23341 ctgtggtcaa gcttactaaa ggaatcaagg cgatatctgt atctgccaaa aatggttcta
    23401 caaagttggc atgttcatga ggcaggtctg ggataatcaa gcccttcaca gctgtatcag
    23461 ccagatcttt gacaaagttc tccacaccgt actgaaagag ggggttgaag taggtcatga
    23521 tgaccagtgg aatctctgtt tcaatggttt tcaaggtttc aactaaagcc tgggtagagg
    23581 tcccgtgggc taaactgcgc aagccagctt cttcgataac aggtccatct gcaacagggt
    23641 ctgaaaaggg aatacccact tcaattgcag agacacccaa atcttctaaa aagtgaattg
    23701 tttcagcaag accatccaaa cctttttcgt ggtcaccagc catgatatag ggaacaaaaa
    23761 ttccttttcc agctgcttta atagcattta atttttttgt tagtgtctta ggcatgagct
    23821 tctcccttct ttgctgcatc tgcttccaag cggtctttta cttgaaccac atccttgtcc
    23881 ccacgacctg ataggcatac aatcatagac ttttctggtc caagttcttt ggccaatttc
    23941 accgcaaagg cgatagcatg gctagattcc aaagctggga taatcccttc cacacgagac
    24001 aagagttgga atccttccaa ggcttcttcg tctgtcacag ggacatagct ggcacgttta
    24061 atatcgtggt agtgagaatg ctctggaccg ataccaggat agtccaaacc tgctgagata
    24121 gagaaggctt caagaatttg accatgggca tcttggagca catccatgag ggaaccgtga
    24181 aggacacctg gacgaccctt ggtcaaggta gctgcgtggt gctccgtatc cacaccaaat
    24241 ccagccgctt cagctccata catggctact gactcatctt ctacaaaggg atggaagaga
    24301 ccgatggcat tcgacccacc accaacacag gctactaggg catctggcag attttgtcct
    24361 gtcaagtcac ggtactgttg tttagcctct cgaccaatga cactttggaa gtcacgaaca
    24421 atttctggaa atggatgagg ccccaaggca gaaccaagga tatagtgggt atcatctata
    24481 ttagccaccc atgaacgaag ggctgcattg accgcatcct tgagcacgcg cgaaccatct
    24541 gtcacggcct cgaccttagc tcccaaaagc tccatacgga agacattgag ggcttggcgt
    24601 ttgacatctt cctcacccat gtagatggta cattccatgt taaagagggc cgcagcagtt
    24661 gcagttgcca caccgtgctg accagcacca gtttctgcga taattttctt tttacccatg
    24721 cgtttggcaa gccaaacttg tcccaaggca ttgttaatct tgtgggctcc tgtatggtta
    24781 aggtcttccc gtttgagata aatcttggct ccgccgatat gctgggtcaa gttttttgcg
    24841 taataaagag gagtttcacg tcctacgtac tggcgcaaaa gctggtttaa ttcctcttgg
    24901 aaacttgggt ctgcctgact ttcacggtag gccttctcca actccaaaac tgctgtcatc
    24961 aatgtttctg ggacaaaacg tccgccgaat tttccgtaaa atccatcttt atttggttcc
    25021 tgatatgcca tgctttaccc tctctataaa tcttctaatc ttttcatgat ctttttgtcc
    25081 atctgtctcc actccgctcg atacatctac tgcataggga gtaaaatgtt gaattgcttt
    25141 tactacatta tcttcattaa ggccacctgc gataaagaag ggctgtgcta gtccagtcgt
    25201 atccagttga ccccaatcaa aggactgacc acttcctgcc acaggggcat caaagagtag
    25261 ataatctgcc tgagaattgg ggacatgccc atttccatct acctgcacag cctgaatact
    25321 ggcacaaggc aaattctcaa ataaatcagc tgccacctga ccgtgaactt gaaccaagtc
    25381 caagccaact ttgtcaatcg cttccagcag ttctacccga cttggtgaaa caaatactcc
    25441 aacctttttc acatctgcag gaataagctt tgccaactca gctgcctctt ctaaagtcac
    25501 ctgtctttta ctaggtgcaa agacaaaacc gatatagtcg gctcctgctg aaacggctgt
    25561 ttccaccgct tctttggtcg atagtccaca aattttaacc tttgtcaatc tgcaactcct
    25621 tgattctctg ggccacattt tctgcctgca taagagctgt ccctaccaaa attccgttaa
    25681 agtatggggc tagtcgttcc gcatcctgcc ctgtgaaaat ggcagattca gaaatgtaat
    25741 agcgaccttc ctcaaagtaa ggggctaaat ctacactggt ctgcaagtcg acctcaaagg
    25801 tagtcaagtt gcggttgttg accccgataa tctcagcacc aagtctgtgg gctacctcta
    25861 gttcagctag attgtgagtc tccactaaga cttccagacc aagctctgtc gcgtagtcat
    25921 acagttcctt gaggcgttct tcggacaagg ctgccacaat aagcaagata actgtcgcac
    25981 ctgtattgcg agcgcggatg atttgctttt catctatgat aaagtctttg ttgagcgtcg
    26041 gaatctctac ctgactggaa atttcccgta gataatccaa atgcccttta aagaaaacct
    26101 catctgtcaa caccgaaatc atcactgctc cgttttcttc ataagtctgg gcctgttgca
    26161 caatatccac atcgagattg atatctccca aactagggct agctttcttg acctcagcga
    26221 ttacctgcaa gcagtcctga tgattcttca aaaattctgc caagcgatag gtctggcgca
    26281 gaggctggat ttgctccagc ttcatctgct ccacctcacg cgccttctgc tctaagattc
    26341 gtgctaaaaa ttcctgactc atttttggta ctcctgtaac agtctgagtt tttcaagggc
    26401 cttgcctcta gcaatcactt gacgggccaa ggcaactcct tccttgatgc tatcaatctt
    26461 accattagca tagaaaccaa gaccagcatt caagactgtc gtttccaaga atggacttgc
    26521 ttcgtttttc agaacgctaa gcaaaatttc tgcattttcc tgagcattcc caccacgaat
    26581 atcttccata gcatagtctt ccattcccaa atcctctgga gtaaagcttg acaagctgat
    26641 ttcgccattt tcaagaagtg caatcttggt tgttccgttc aagccagctt catccaaccc
    26701 ttctggtcca gcaaccacga tggcacgttt gcgacccata tttttcaaaa cctgagctgt
    26761 actttctagg agttctggac gactaattcc aagaagctgt gtttctaaag ccattggatg
    26821 aatcagtgga ccagtcaagt tcataatcgt tggaattccc aattccaaac gagctggcat
    26881 gatgtatttc atagctgggt gcatattttt agcgaagaga aagacgattc cagttttatc
    26941 aaagacctta cctagttcag ctggtttgag gtcaagattg attcccaagg cttcgaggac
    27001 atctgcggaa ccagatttag aagaaatcga gcggttacca tgcttggcca tgtgaatacc
    27061 gccaccagcc aagacaaagg ctgcagttgt ggaaatatta aaactgaaag acttgtcccc
    27121 acctgtacca cagttgtcca tagcatcatg aatctcagtt ggaatatgct gggcatgtcc
    27181 tctcatgact tgggcaatgg ctgtacgttc ttcaggtgtt tcccccttca tcttaagagc
    27241 taagaggaga gaagcaatct gcgcttcagt tacacgccca gttacgatac gctcaatgac
    27301 atccgtcatt tccacacctg ataaattttc aaattttgct agtttttcaa taatctcttt
    27361 catcctagtt tcctcacttt acaaccttct cgataaaatt ccgaatagaa gacaagccgt
    27421 ctggcgttcc aatgctctct ggatggtact ggaagccata aatcggtagg tttttatgtt
    27481 gaatccccat gatggcttgg tcatcagtcg aacgagctgt cacttcaaag tcttctggca
    27541 tttcctcaat caaaatactg tgataacgca tgaccgcacg tccatcctca ataccttgat
    27601 acaaaacaga tggcgcttca aagttgatat tgctctgttt cccatgcatg acttttggag
    27661 ccaaacctag cttaccacca aagacttctg caatggcttg gtgacccaaa caaatcccta
    27721 gaatcggctt cttgcctgca aaatcacaaa tcatgtcttc catctttcca gcatcaactg
    27781 gccaaccagg accaggagaa aagaccagac catctgcttt ttcagcttct tcatacagct
    27841 tggaatcatc atttctcaaa acctgaactt ctgcaaaatt cccaatgtat tgggccaagt
    27901 tataggtaaa agaatcatag ttgtcaatca ataaaatcat ggtcttagtt ctccaattct
    27961 agtcatagat tttgctttgt taatggtttc ttggtattcg ttttgggcga tagagtcgta
    28021 gacaatccct gccccagcct gcacataggc tctttgattt ttgagaatca tggttcggat
    28081 ggcaatggcc aaatccatat cacccgtcgc agacaagtag ccgattgctc ctgcatatac
    28141 gctccgtttt tccgtttcca gttcatagat gcgtctcata gctcgaattt ttggtgctcc
    28201 agaaacggtt ccagcaggaa gcgttgcttt caaggcatcc atggcagtga gttctgcaag
    28261 caaacgtccc ttgaccacac tggtcaaatg catgacgtag cggaagagct ccacctccat
    28321 atacttagta acttggacac tggccgtttc agagatgcgg ccaatatcgt tacgccccaa
    28381 gtctaccaac attcgatgtt ctgctgtttc cttctcatca gagaggaggt cagtcgccaa
    28441 ggccttgtct tcttcatccg tagcccctct tggtcgcgtc cctgcaatcg gattggttgt
    28501 cacgatgcca tttttgacag aaaccaaact ttctggacta gctccgatga tttgataatc
    28561 cccaaaatca tagaaataaa ggtaattaga tggattagtc acgcggagat ttctgtagaa
    28621 gtcaaatgga tttccagtaa cttctgctga gaaacgctgg ctgagtacac attggaacat
    28681 atctccgtta cgaatcaagt cacgagctgt ttctaccatt ccctcaaact tatgtggagc
    28741 gatatgcggt ttgaagtcaa gtggtgataa atccaagtct tcaaattcat ttggagcagg
    28801 aatgcgtaat tcctcaagca cttggttcaa ggatttttcc aaggcctctt gactgcgctc
    28861 actataaagt gcatcctcta tgacatgtat cttctccttc ttgtggtcaa agaccatata
    28921 gctctcatag acaaagaaat gcatgtctgg cgtcccaatt gtatcctcag ggatttgacc
    28981 aatttcttca taaagcgaaa tcatatcgta accaacaaaa ccaatggctc ccccaccaaa
    29041 agggaggtct gaatggtgct ggctcttatg agtcacttca taaaggaaat ccaagggatc
    29101 ccgatcaatc gcttgaccat tttgatagag aactccattt tcaaacttaa tctcaaaaac
    29161 tggattatag gctaggatag aaaaacgagc tgtttccttg tctctcggaa tactctctaa
    29221 aataacctta tgttgcccct ttaagcgcat ataagccaag attggtgata agacatctcc
    29281 atgaatgatt cgttccattg tcatttccct ttcagttcta attctagttc gtggcgactg
    29341 tatgaaaaat ccccacgcaa aataacttgc gtgaggacga aattcgcggt gccacctcaa
    29401 ttataggatt tctcctatct ctcattcctg tctcagatat ctcctgtaac aggctgtgcg
    29461 ataaagggca ctcccttgag aatgatgttt tcttctctcg tttcagatga acccaacttt
    29521 acagctttct ctgcttgttt tcagcaacca caagctctct gtgagagaaa agactgtaat
    29581 ttttccatct attatttttt agcttctagt aatctgcaat cgcagctagg tccttgcctc
    29641 cacgaccaga gacattgatg aagagatgtt catctcggta cacctttata ctcttcgaaa
    29701 atctcttcaa accgcgtcaa cgtcgccttg ccgtaggtat ggttactgac ttcgtcagtt
    29761 ctatctgcaa cctcaaaaca gtgttttgag ctgacttcgt cagttctatc cacaacctca
    29821 aaacagtgtt ttgagcaacc tgcggctagt ttcctagttt gctctttgat tttcattgag
    29881 tattactagc ttttttcgta ttagtccagc ctttttgttt acttttagta ggaggcaagg
    29941 agctgtagat agaactcaag ttcatcaaag cgacttaagg ccctaataaa agataaacca
    30001 aacgacggat agaaaaaagc ccacacacag aatatacttc cgtgtgaggg cgttggtaac
    30061 gcggtgccac ctcaattata aagggactat ccctttacat ctctgccttg tttaacaaca
    30121 agctgcactg taaggtgtgc gcaccgaatt ttcattgttt caaattcatt ttcaaaatca
    30181 gcccactttc actacttcca accacctatt cacaatcacc acaggctccc tgaagatcaa
    30241 aaatagttac ttttctgatt tgttgaactt attttaatac tttgtttttt ctttgtcaag
    30301 acttttttac gatttttttg atatcattcg aatgtgacca tatcttcctt agatcgaaca
    30361 tgaacatgtc ccacttgtta gaaattggat ccaactcaat agaaactgaa tggaggctaa
    30421 acagaactta ttttagaaca ctccatcttt tccactagga ttttcaagaa ttaaacaata
    30481 ctagaaactc tgtctcctaa caaatttagg agaaacttca acagatgtga cactttcccc
    30541 tttaataatt gctaaaacac cttctatcat ttctttagcc aatttaacat aattgggagc
    30601 aattgtagac aaagctggag tataatactg agaaatagga atattatcaa atccaatgat
    30661 agaaatatca tctggaataa gaattccttt ctcatagcac gcacgaatca agccctgaac
    30721 cttttcatct cctgaaacaa aaataatgtc cggataattt tgggtagtca agtgctgcat
    30781 tgcataagaa taaactgaat caattgtaga taagccataa atgactttta aatccataaa
    30841 gtaattttta tcattcagaa aagaacgcac acctctttca cgatccttat taacatggga
    30901 ttctcctccc ataagcaacc acatattttt aaatttttct tcagttacag ctttcatcat
    30961 atcataagta gcttgaaaat tattattaga tacatagact actccagacg tttgagattc
    31021 accgaaaaca agaaaaggca tatggttctt ctttaaatac tgaattctga tatcatctac
    31081 actttcataa aaaacaataa caccatctac taggctacct gtgcttgata taattgaatt
    31141 actaattgta tcctcctctc caaagtactc aactatagca ttaacaccaa attctttaca
    31201 cgtccgtaac actttatcta acagcgtatg aaaccaaatt aaaggaaaag agtcgatttt
    31261 ttttacagaa atcaatatat ttatagcttc ttttttagtt aaattttttg catacgcatt
    31321 tggaatatac gacaattcct ctataacttt ttgaatcgct tgataagttt cttctttaac
    31381 atttactcca ccattaataa ctcgtgaaac tgtttttgga gaaaaacctg ataaacgtgc
    31441 aatatcataa atagttacct ttttcccatt tatatttttc atttcagtcc tccattacga
    31501 acattctaat attactatac aatatttaat tttttttaac aagagaattt agtaaattat
    31561 ttaagatcca caaattcaca aaattaattt tacaaatatt cttccccttc aaaaaaattt
    31621 aaattgcatt tcacaccttt atttttaaga atgtttccaa cttcacgaca aataaattca
    31681 tatgagaaaa aactgccata aaattgtaga ttaacttttt cagtaaaatg tgtaggattt
    31741 ataaaaacat ataatagcct gtcaatgtaa cattttaaca tagagttaat tttttcttta
    31801 aagataacat ttgttatcaa ctcatcagga ggtaaatgaa aggcaaacac catttcacaa
    31861 atatcataaa aagaaataaa tttgtatact tgtatcaaac aattattatc aaaatattct
    31921 attttaccta aatcaaaatt gattttataa tctttcataa aaacctctga gcaaaaatct
    31981 actcaaaaat tagatgatta aaacatctaa aaaacaaaag gacaaaaaca tctgtccctt
    32041 tgtttactaa atttcagcta atttcttcga cataaataac acctacaata ttagcaattt
    32101 cttccatcag tcgaagatgt tcaaatctac ctgataattc cagagtaata aatgacgcta
    32161 tttttttatc tggaacatca aagtattcaa ttctgtcaga attaacatct ccaaacgctg
    32221 ttcttgaatc ggtcattctg ataccatttt ctgcacaata aaccaataca cgattatagg
    32281 cttctgtaga tttaaccact atatacaatt caatcatttt agaacgattt tgtagatatt
    32341 tttttagtgg ttggaacatg gatatcacac cccaaacaga aatggctact aaaagagctc
    32401 cctcataaaa acctactcca atagctaatc caattcctgc cgaagcccaa atgcctgctg
    32461 cagttgtcag acctgtaatt ttctttttat ctgtaataag aatcgttcca gcgcctagaa
    32521 aacccacacc tgatataact tgagctccta atcgtgtagg atctcctgtc ccaaatttat
    32581 aagatacgta ttcattcgtc atcataatca aacatgcagc tagacaaaca atactataag
    32641 ttcggatgcc tgcaggctgg gatttgctcc ctctctctaa accaattata ctaccaatga
    32701 ctactgataa aacaatcctg acaactattt caatatttga taacccaaga ctagtggctg
    32761 tcatgattat ttccttactt tacgccccgg tctttgtgtg aagtataata ccgttccaga
    32821 aataatcatc agaacaattg tataaacaaa taccagagct tgtgcattag atgttgctgt
    32881 ttcatcacct gcagatcgaa tcgtaatacc taatggttga gctaggggat ggtaaaggaa
    32941 tacagataag tcgaagtcag ttaataaaga gttaaagttt aaagcaataa cagagagaac
    33001 aaccggtaaa ataaatggaa tgataacctt catcatagta taaaaaggtg aagcacccat
    33061 acttcttgct gcatcttcca tctcatcatc aacactaaat aaaatagcac gtaccattct
    33121 ataagaaaat gggattttta caactatata tgcaataagt agaattacca aactacctac
    33181 caaaatctga ttcaagacaa gaaattgtgg ctgattaaaa gtaaataata aacttactgc
    33241 taaaagcgta cttggtaata accaaggaag tagagcacca tattcaaata agaaatcaaa
    33301 acgagattta tgttttctga caacacgagc aaatacaact gcgagaattg ttgctgttgt
    33361 cgcagcaata atagaataaa taaagctgac caagaatgga gagaatgccg cactattact
    33421 aaagaataag cgataatttt ctaaagtaaa gtttgataat gttaagttac ctgtttgaat
    33481 tgcaactgga tctgtaaatg agtataatac tataaaaatt agtggaagca tgaaaactgt
    33541 gaacaatcca tatgctacaa tgtgagcaat gatattccaa ggcttagacg caattttttg
    33601 ttttttaaga ggcgctttag tcttagagat agaaatataa tttccacctt tttctatctt
    33661 attcatgata gtaagcaaaa ttgtagttgc aatacctaaa ataattgcaa gtaaggcagc
    33721 taaatcacga gaattcccca tccctgcaaa tgtaataatc attggattta tagtttgaaa
    33781 ttctttacca ccaacaatca tgggtgctgc tactgcagat aaaccactaa gaaaaaccat
    33841 aatagtaagt gcaaatagag ttggaattaa ggttggtaac actacttttc ggaaaacagt
    33901 aaatggtttt gctcccatat ttcgagcagc ctcaatagtg tgatagtcaa cgcttcgaat
    33961 tgtatttgtt aaaaacaatg tatgattagc agttcctgaa aatgtcataa tgaataagac
    34021 tgcaccatac ccaataaacc agttagggtc taaagaaggg ataacatttt gtaaaaattt
    34081 tgtaatcaat ccatatggac catagacaaa tttatatcca gtcgctaaaa ccactcctcc
    34141 ataaattaaa gaggtcatat aacctaattt taaaatttta gcacctttaa tatcaaagta
    34201 ctctgtaaat agaacacaaa gaatacctac gacattaact gtaataatga gtgaaaatgc
    34261 taacttaaaa ctgttcataa tactctgaag tgccctctga gattttagaa cacgatgtac
    34321 agcatcaagg gaaaattctc ctccttttac aaatacattc actactagat caaagtttgg
    34381 ataaataata aatgttacta agaaccagat taaccctaaa cgaataagcc aatcttttaa
    34441 atttaattta tgacgcatac tgcacctcct taaaattgca gaacgtctga tggtgtgata
    34501 aataattcca cactttctcc gacagatcta atagcagcct gactatcaat acttgttaca
    34561 ttaagaatct gactttcaga aacttttatt gtatagtgaa ttgtaactcc agaaaactca
    34621 acatcaataa ttgtcccttt tagaatgaaa tcttgttcag tttcacgatt gaatcgaact
    34681 ttctctaatc gaatgtatcc ttttttatct tctaagaaaa cgcttgtatt tttcagtaat
    34741 acttcgtgga ctgtttcatc ggtcaaaaca ttaatatctc caataaaatc acatacaaat
    34801 tcagtttgag aattatgata aatctctact ggtgtaccga cctgttcgat gtatccattg
    34861 ttaaagactg caattctatc agataaagtc aaggcttcct cttgatcatg agtaacatat
    34921 aaagtagtaa tacctaactc tttttgaagt cttttcaact cttttctcaa atctacacgt
    34981 aattttgcgt caaggtttga caatggttca tctagacaaa gaattttagg ttcaagaacc
    35041 agagcacgag ccaatgctac cctttgttgt tgacccccag ataattctga tacattacgc
    35101 tgtaactgtt gatcagagat cttaattttt gctgccactg ctgatacttt agctttaata
    35161 acatctggag ctaccttctt aacttttaaa ccaaatgcaa tattatcaaa aacagtcata
    35221 gttggaaata gcgcataaga ttgaaataca ataccaattc cacgcttttc aggttccaaa
    35281 tgagtgacat ctgttccatt aacttcaata cttcctgatg atggatctaa aaaacctacc
    35341 aatgctctca aagtagttga tttaccacat cctgaaggcc caagaaatgt aaagaattcc
    35401 ccttcatgta tatctaaatt cagattatca attgcaacaa aatcaccata tttaatttga
    35461 atattatcaa atttaatcat ctcactaact ccctctatta ctaaaccaaa agcctctctt
    35521 tatttcttcc ttaaatttag aaataataga gagacttgga cataaaaaat aactcttatt
    35581 tcttatttta cgtattctaa ttcagctttt tctacccatt catccaaatg ctttccaaca
    35641 gcttcccagt caatattttg tggtttcact tgatcaacaa attttttcgt atcttcaggt
    35701 aactctttta gggcatcttt atttgcagga atagatccaa attttttact atattctact
    35761 tgaatttctg attgaccaaa ccaatcaata aattctttag ctaacgcttg ttttttacta
    35821 gtgcttaaaa ccatagtttg ttcagttaca aatggtacac caatctcagg agtcataact
    35881 ttgaattcaa cattttgttc tttttgtccg attaacgctc cagaacccca gatcataccg
    35941 tattgaattg ggctttcttt atctaacgct ttaacaactg agctttcacc tttttgaaga
    36001 gtataggcat ttgcaaagta ttcttttgca acttcccatc ctttttctga tacgcctaaa
    36061 tctccttttt catcaaggta acgaactaag atacttgcta gaattgcccg tcctgtacca
    36121 ccttgaagac cataaattga atatttacct ttatacttac tacctaattc agtccaatct
    36181 ttaggcattt cttttacatc aggcgcccca attaaaacta atggttgaac aatcacagga
    36241 ttataataat tatctttatc tgataaagat tgatcaattt tatctaacca tttaggcttg
    36301 tactgtacta gtaatttttg atctctaatt ttatttgagt caacagcacc aattccaaat
    36361 accatatctg caactgcatt attcttctca gcaataacac ggtctgctaa ttgagcgcca
    36421 gcaatatcaa ccatttttat attaaaacca gcttcttttg ctttagcagt taaccaatca
    36481 ccacgaccat ttgagactga gttcgaatag attactaatt cttgactttt atcagctttt
    36541 tcttcagatg aagaagcagt cgtagaattt gaacctccag agcaagcagc aagtgtagta
    36601 agagcaactc ccgttgcaag tacagtagac caaactttca tttttttcat gataagttct
    36661 ccttttttat tattttattt aaatttttcg tgatatggaa caaattgtct catatcttca
    36721 aatacagtat agtcaatacg gtttacagta atagttggaa tcttctctaa taaaatttca
    36781 gttaattctg ctctgacttt agtaaactct tcttcctcct cttcggttag aggaatccga
    36841 agatacccaa ttgaaatatg gaattgatat ctatcatgat tagggaaaca aacacctgct
    36901 ttttctgaga cataagtacg aatttcttct aatctctttg cagaagcttc atctgcaggt
    36961 tcaactagta tgttttgttt tcccatttca gttatacgca tatgaatttc ttcatccaac
    37021 aatggaaaaa tttcaagttg tttagcaaag taatcatgta tttcctgtaa aggtgtatct
    37081 agaggaagat tactgctcca aaactccgtt tcacgatttt catggcacaa caattcaatt
    37141 acagtcatgt gaatagaatt ccttggagtt aaagtaaact tatcgataaa tggtaattct
    37201 ctataacgtg attgaataat atcaacaact tccatcaaat cttgtttagt ataaagattt
    37261 gctacaactg tattcccagg gaaatgatta aattccccat tctctttgaa cttaattttt
    37321 gttaagtttt tcatataaag accttctttt ttatatttga catcggtgtc attgtgttta
    37381 cattatacaa tgaaaccgtt tcctttgtca agcgctgtca aacatttttt ttaatttatc
    37441 tttcattaaa ag
//
LOCUS       DAVBCB010000015        37229 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVBCB010000015 DAVBCB010000000
VERSION     DAVBCB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.015"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            175..1938
                     /gene="aspS"
                     /locus_tag="VQU28_000739"
     CDS             175..1938
                     /gene="aspS"
                     /locus_tag="VQU28_000739"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HET2476123.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVVNATLGTLRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSTEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            1916..2857
                     /locus_tag="VQU28_000740"
     CDS             1916..2857
                     /locus_tag="VQU28_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2476124.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(2977..3057)
                     /locus_tag="VQU28_000741"
                     /pseudo
     CDS             complement(2977..3057)
                     /locus_tag="VQU28_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3193..4179)
                     /locus_tag="VQU28_000742"
     CDS             complement(3193..4179)
                     /locus_tag="VQU28_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2476125.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            complement(4189..4989)
                     /locus_tag="VQU28_000743"
     CDS             complement(4189..4989)
                     /locus_tag="VQU28_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HET2476126.1"
                     /translation="MLPYPFSYFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTASQAPSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRIYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            complement(5250..6092)
                     /locus_tag="VQU28_000744"
     CDS             complement(5250..6092)
                     /locus_tag="VQU28_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2476127.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(6094..7386)
                     /locus_tag="VQU28_000745"
     CDS             complement(6094..7386)
                     /locus_tag="VQU28_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2476128.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGNKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(7497..8768)
                     /locus_tag="VQU28_000746"
     CDS             complement(7497..8768)
                     /locus_tag="VQU28_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2476129.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            9318..10835
                     /gene="malQ"
                     /locus_tag="VQU28_000747"
     CDS             9318..10835
                     /gene="malQ"
                     /locus_tag="VQU28_000747"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HET2476130.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLEASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEYFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            10861..13119
                     /locus_tag="VQU28_000748"
     CDS             10861..13119
                     /locus_tag="VQU28_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HET2476131.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIDLDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(14163..14735)
                     /locus_tag="VQU28_000749"
     CDS             complement(14163..14735)
                     /locus_tag="VQU28_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476132.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     FAINLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(15357..16205)
                     /locus_tag="VQU28_000750"
     CDS             complement(15357..16205)
                     /locus_tag="VQU28_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HET2476133.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            16350..16628
                     /locus_tag="VQU28_000751"
     CDS             16350..16628
                     /locus_tag="VQU28_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476134.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            16630..18693
                     /locus_tag="VQU28_000752"
     CDS             16630..18693
                     /locus_tag="VQU28_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HET2476135.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(18771..20027)
                     /locus_tag="VQU28_000753"
     CDS             complement(18771..20027)
                     /locus_tag="VQU28_000753"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HET2476136.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADENNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            20169..22634
                     /locus_tag="VQU28_000754"
     CDS             20169..22634
                     /locus_tag="VQU28_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2476137.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            22699..23610
                     /locus_tag="VQU28_000755"
     CDS             22699..23610
                     /locus_tag="VQU28_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HET2476138.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCLEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            23840..24538
                     /gene="dapD"
                     /locus_tag="VQU28_000756"
     CDS             23840..24538
                     /gene="dapD"
                     /locus_tag="VQU28_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HET2476139.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVCVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            24606..25736
                     /locus_tag="VQU28_000757"
     CDS             24606..25736
                     /locus_tag="VQU28_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HET2476140.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFTSQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            25748..26287
                     /locus_tag="VQU28_000758"
     CDS             25748..26287
                     /locus_tag="VQU28_000758"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HET2476141.1"
                     /translation="MKSELRKQVLHEMKAISQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLTPKTYPKGRMDFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDASQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRALSTIYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            26271..26948
                     /locus_tag="VQU28_000759"
     CDS             26271..26948
                     /locus_tag="VQU28_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HET2476142.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(27020..27919)
                     /gene="galU"
                     /locus_tag="VQU28_000760"
     CDS             complement(27020..27919)
                     /gene="galU"
                     /locus_tag="VQU28_000760"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HET2476143.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(27941..28957)
                     /locus_tag="VQU28_000761"
     CDS             complement(27941..28957)
                     /locus_tag="VQU28_000761"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2476144.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     YHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKAI
                     IMHASKGLEPDSHKRLSTILEEEIPEQLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            29169..29546
                     /locus_tag="VQU28_000762"
                     /pseudo
     CDS             29169..29546
                     /locus_tag="VQU28_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29726..30151)
                     /locus_tag="VQU28_000763"
     CDS             complement(29726..30151)
                     /locus_tag="VQU28_000763"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476145.1"
                     /translation="MNNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKSLTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(30219..30869)
                     /gene="phoU"
                     /locus_tag="VQU28_000764"
     CDS             complement(30219..30869)
                     /gene="phoU"
                     /locus_tag="VQU28_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HET2476146.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(30884..31636)
                     /gene="pstB"
                     /locus_tag="VQU28_000765"
     CDS             complement(30884..31636)
                     /gene="pstB"
                     /locus_tag="VQU28_000765"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HET2476147.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDVLLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(31638..32453)
                     /gene="pstA"
                     /locus_tag="VQU28_000766"
     CDS             complement(31638..32453)
                     /gene="pstA"
                     /locus_tag="VQU28_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HET2476148.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(32446..33309)
                     /gene="pstC"
                     /locus_tag="VQU28_000767"
     CDS             complement(32446..33309)
                     /gene="pstC"
                     /locus_tag="VQU28_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HET2476149.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRTFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(33427..34302)
                     /locus_tag="VQU28_000768"
     CDS             complement(33427..34302)
                     /locus_tag="VQU28_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HET2476150.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(34469..35800)
                     /locus_tag="VQU28_000769"
     CDS             complement(34469..35800)
                     /locus_tag="VQU28_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HET2476151.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGQGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSALKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(35801..36508)
                     /locus_tag="VQU28_000770"
     CDS             complement(35801..36508)
                     /locus_tag="VQU28_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476152.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGMEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(36592..37077)
                     /locus_tag="VQU28_000771"
     CDS             complement(36592..37077)
                     /locus_tag="VQU28_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HET2476153.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            37063..37229
                     /locus_tag="VQU28_000772"
                     /pseudo
     CDS             37063..37229
                     /locus_tag="VQU28_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT        10914 a         7064 c         8026 g        11225 t
ORIGIN      
        1 atacctacgg taaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattagcgct
       61 aatttttagg gctttcacct gacttttaaa gactggcaaa cttttctgat gattttcatg
      121 aagagcctgc gcttttatgg taaaatagta acagaataaa agaggagaga aataatgaaa
      181 cgtagtatgt atgctggtcg tgttcgtgag gaacacatcg gacaagaaat aaccttgaaa
      241 ggatgggttg gccgtcgtcg tgaccttggt ggtttgatct ttatcgatct tcgtgaccgt
      301 gaaggaatca tgcagttggt tatcaaccct gaaaaagtat ctgcagaggt tatggcaaca
      361 gctgaaagcc ttcgtagcga atttgttatt gaggtgactg gtcaggtcgc tgcgcgtgag
      421 caagccaatg ataagttgcc aactggtgcg gttgagttaa acgtgacagc tcttattgtg
      481 cttaacacag ctaagacaac accatttgag attaaggatg gcattgaggc aaatgacgat
      541 acacgtttgc gttaccgtta ccttgacctt cgtcgtccag aaatgttgga aaatcttaaa
      601 cttcgtgcca aggtgaccca ctctatccgc aactatttgg atgagttgga gtttatcgac
      661 gtggagacac cattcctttc taagtcaacg cctgaagggg cgcgtgatta tttagtgccg
      721 tctcgtgtta ataaggggca tttttacgct cttcctcaaa gtccacaaat cacgaaacag
      781 ctcttgatga atgctggttt tgaccgttac taccaaatcg ttaaatgttt ccgtgacgag
      841 gacttgcgtg gagaccgcca gcctgaattt actcaggtcg acttggaaac gtccttcctt
      901 actgagaaag aaatccaaga tattacagaa ggcttgatcg cgcgcgtgat gaaggaaaca
      961 aaaggcatcg aagtaacgct accgttccct cgtatgaaat acgatgatgc tatggctctt
     1021 tacggttctg acaagccaga tactcgtttt gacatgttgc ttcaggactt gacagaagtg
     1081 gtcaaaggtg tagactttaa agtcttttca gaagcacctg ctgtaaaagc gattgtggtc
     1141 aaaggagctg cggacaacta ttcacgtaaa gacatcgaca agatgacgga agtagccaaa
     1201 cagtatggtg ccaaaggtct tgcttgggtc aaggtggttg atggagaatt aaacggacca
     1261 gttgccaagt tcttgactgg tatccaagaa gaattgacaa cagcgcttgc tcttgaagat
     1321 aaggacttgg ttctctttgt ggcggatacg cttgaagtgg ttaatgcaac actgggtacc
     1381 cttcgtggac gtattgctaa agagcttggc ttgattgata atgataagtt caacttcctt
     1441 tgggtggttg actggccgat gtttgaatgg tctgaagaag aaggccgcta catgagcgcc
     1501 caccatccat tcacacttcc acaggaagag actgctcacg aattagaagg tgatttggct
     1561 aaggttcgtg ccattgctta cgatatcgtc ttgaacggtt atgagcttgg tggtggtagc
     1621 cttcgtatca accaaaaaga ccttcaagaa cgcatgttca aggctcttgg tttctcaact
     1681 gaagaagcca atgaccagtt tggtttcctt cttgaagcca tggactatgg tttcccaccg
     1741 cacggtggtt tggctatcgg gcttgaccgt tttgtcatgt tgcttgctgg agaagaaaat
     1801 atccgtgaag tcattgcctt tcctaagaac aacaaggcaa ctgacccaat gacacaagct
     1861 ccatcaacag tcgctctcaa acaactagag gaactcagct tacaagtaga agaagatgaa
     1921 acaaacaaaa cgaattaagc ggtggcgcta ttatctgcgc cgctttgctt atcagataaa
     1981 aattttacgt gtcttacaaa gtatctctcg agaaaagtat gatgagaaga tttcggcctc
     2041 tctggtctat ggttttttat cagcagtagc agttaatttc tttttccaac cagggcatgt
     2101 gtattcgagt ggtgcaacag gtctggcaca gattatctct gccttgagta atcactggtt
     2161 tggttttcat attccgattt cgctaagctt ctacgccatt aacttccctt tgatggtctt
     2221 agcttggtat cagattggcc ataagttcac cgtctttacc tttatcacgg tatctatgag
     2281 ttccttcttt atccagtttg tccctgtggc aaccttgacg gaggatccca ttatcaattc
     2341 cctttttggg ggtgttgtta tgggtttggg gattggtttt gctcttcgaa acaatatctc
     2401 cagtggtggg acggatatcg tcagcctgac tattcgtaag aaaacgggta agaatgtcgg
     2461 tagtatttct ttcttggtaa atggaactat catgctgata gcaggtttga cctttggttg
     2521 gaaatacgct ctttattcta tgattaccat ctttgtctct agccgtgtga cagacgcagt
     2581 ctttactaag caaaagcgta tgcaggccat gattgtgaca aatcatccag agaaggtaat
     2641 tgaaaaaatc cataaaaaat tgcaccgcgg agcaaccatg atccacgatg cagaaggaac
     2701 ctataatcac gagagaaagg cagttttaat cactgtcatt acacgtgcag agtttaatga
     2761 atttaaacag attatgacac aagtggatcc aagctccttt gtctctgtct cggaaaatgt
     2821 tcatattcta ggaagatttg ttgagataga taattagtga ttaatactct tcgaaaatct
     2881 cttcaaacca cgtcagcttc accttgccgt atatatgtta ctgacttcgt cagttctatc
     2941 tgcaacctca aaaccatgtt ttgagctgac ttcgtcagtt tcatctacaa cctcaaaacc
     3001 atgttttgag caacctgcgg ctagcttcct agtttgcact ttgattttca ttgagtataa
     3061 aaagatcttg ggaaaagact caatttcagt agaaaaggaa gtaaatcttc ccacattaaa
     3121 acgcatagta tcaagttttt tcaatacctg atagtatgcg tttttgcttt taacgacttt
     3181 ttgcccagtc tcttattttt cgataatttt gtgggcgatc aattgacggt aacaaatttg
     3241 tttattgttt ttattatcat taataatctg gagaatagtt tcaagggaaa cacgaccaag
     3301 ctctaaacta ttgatatcga cataggctgc caagttgagc ttgggattaa ccgagtcaaa
     3361 gctgagaaca gggacatcca gctggtgttt ggcaatatag ttacaaacgc cttcagctag
     3421 gaggctatcg gttgtgatga tagcatcaat ttgtggatcg tgcttgaata atcgcttgct
     3481 aaatttatag cccttttctt ccagaaactc gtcggcaaag tagatgcgat tgttgtcagt
     3541 ggtaagtttg taatgtttaa gcgcctgttc atagcctgtt aaacggtctt tggtcacgaa
     3601 gagcttttta cttcctccga taaaggcaat gcgtttgcag ccttttttga tgaaatattc
     3661 agtcgcatca aaaccagctt gaacattgtc gttgtcgaca agtgggatga aaggagatag
     3721 agatttacct aagataagga aggggaactg ttcttctgcg acgagtttta cgagagggtc
     3781 ttcttcttgg gcatagagaa agattagccc atctacacgc ttgccgtaga ccatttgtga
     3841 aatagcgttg agacgctcct tctcatcttt ccctgttgct atctgaatgg catagtggtt
     3901 ttcagatgcg acttgagaga tgccacgtag aaccgatgga aagaaaggat tctggtagaa
     3961 ggcgtctgag tcatcaggaa gaactaatcc gataacctga gtatagctgc ttaccaagct
     4021 acgagcgttg aggtttgggt ggtagttgag ttccttcata gctttgcgaa cgcgtttttt
     4081 tgtttcgtcg ctaatggttg atttattttg aataacacgg gttacggttg aaggcgaaac
     4141 accagcagcc ttggccacgt ctttaatcgt aacgggcata aaaatctcct atttatggta
     4201 atttggatca cggaaatgtg ttttataaaa gatagtgacc agatacagaa caaaaagaat
     4261 attttgtaca gtaatcaggg ttgtcatatt ttggccaaat aatcccaaaa taagtgtaat
     4321 cagagtcggc aatcctaaac agttcaagat aaagtgatag cactctttaa aggttctaaa
     4381 tgaaaagagg cgtgatttct tagtgatata aaggagaaga ctagctccta gagagacgat
     4441 aaagaaattc aaaccaaaga ggaagctcgc accgagaact aggaagaggc tgatatagat
     4501 acgattttgt tggtaccagt ctttagaaat tgcttgggtc aagctgtctt tgcttttgaa
     4561 actctcagtc tgaatggctc ggtaagagat gcgggtcagt tccttgcttt ccttgctgat
     4621 gactagttca tttgtatcga aatgcagttg caagtcctta ggtaattcct tgatttgact
     4681 tggaccaatc acaacagaag gggcttgact agctgttcca gtataagtta atgtgccatc
     4741 gacaattgta gcatgttcag agagatcctg gacaacctca tttgtcagag gttcatagac
     4801 attatcgata aaagtttcta gcggataggt ctcctgggag ctgttttgga tagcaatggg
     4861 taccatagac aagctgataa ggaagatact ggtaaagaga agttgaaacc agttgagctc
     4921 gaaacgtttg gacaggggct tacgaaaccc ccaaatactt gaaaaatagg aaaatggata
     4981 tggaagcata ggtatctttc taaaaggtgt tttctatatg agtattatta tatagagaaa
     5041 tgcgctttat ttcaagtcta ggagaaaaat tgcttgctac aaggatatta tttacagtga
     5101 caattgctaa taatcaatga aaatcaaagt gcaaaccagg aagctagctg caggttgctc
     5161 aaagcacaac tttgaggttg gagataaagc tgacgtggtt tgaagagatt ttcgaagagt
     5221 ataaaatgaa aaagggtggc ggggataaat tatcccttgt cgccaccact tgtaagtcct
     5281 gaaacaaagt tcttttgtag aaagaagaag agaatacaga ttggaagggc gatgaggata
     5341 gcacctgctg agaagtaggc aatcttcaag tttttcgcat tgttaacgaa ggtttggaga
     5401 cctacggcaa cagtaaagta ttctttctca cgaagcaaga aactagagag gatgtagtcc
     5461 ccgaaaggtc ccatgaaggc ccagagagct tgtacggcaa ccattgggcg aacaagtggt
     5521 agaacaattt gccagaagcg gcggaagtgt cctgcaccgt ctagttttgc agattcgtct
     5581 aaagacattg gcactgtatc gaagtagcct ttcatgagcc aagcattcat cgggatacca
     5641 ccaccaacgt agaggaagat gaggaaccag ttgtggttaa gggcgttcaa cataagcgcc
     5701 ataacgaaga aggctgtcaa agcggccata gttggcacca tttggatgat caagaagaag
     5761 accaaacttt gtttacgagc caagaagttg taacggctgt aagcataacc agcaagtacg
     5821 atgatacttg tttgaacagc catggtaatt aaggcgataa tcaaagtgtt gaggtaccaa
     5881 gtaccgtaca aggtttcagt gaagaggcct ttaaagttat caaaattgag gtcgatatta
     5941 gtatctagtt taaaggctga gacgttacct gctttaaagg ctgacataat ggtaatcaac
     6001 agtggataga taattacaat tgatagacca atcaggtaaa ggtaagtaag gctttgagtc
     6061 agtctacgtt tgagtttaat tgagttattc atcttagacg tcctccatat caaatgcgtg
     6121 tagtttcttg aatgcgatca tagagattga gatgacaatg atagagataa tcaaggtaac
     6181 agctgccgcc attgagtatt gaggagatgt acctgttgtc aaacggtaga tccatgagat
     6241 caagatatcg gttgaaccag ctccacctcc gacactacca ggtcctccac cattgaagag
     6301 gtacatgata gagaagttgt taaagttgaa ggtgtattgg ctaatcaaag taggtgccgc
     6361 aacagccaaa atcattggga aagtgatgtt gcggaatttt tgccaagcgt tggcaccgtc
     6421 aatataagct gcttcgtaaa ggtcgttagg aatagattgc aagataccca aagtcaaaac
     6481 gtagatgtat gggaatccga gccaaccttg catcataatc aaggcaatct tagtccaagt
     6541 tgggtctgtt ttccaaggaa taagagctcc atcaaggaaa ggaaggaatt tagccaagat
     6601 tggcaatact tgagtgttga tagcaccgac actatcgtta aacatgtttg agaatgtcaa
     6661 gatagtgatg aaggctggga cagcccaagg aagaaggaaa ataacaccaa agatacgttt
     6721 tcctttgata aatggttggt tggcaatgat agctgtgaag ataccaatta cgatttgtaa
     6781 agtagaagct gccaaagccc aaatgatagt ccaagaaaga acagaaccaa aggcagaacg
     6841 gaaggtactc aagctccaaa tgtttgtaaa gttggtcaaa ccaacccagt ccaacaactt
     6901 gtttggtggc aagtgttgga agtcgtagtt ggtaaaggcg atcatcaagg ttacgataac
     6961 tgggaagata atcgcgaagg tcatggcaac ataagatgga atgatcaaga ggtaagggaa
     7021 gccattttca taaatccctt tgatcatgtc tttgagtgtg cgtggaactg gaattccatt
     7081 gttaatgcgt tttgcaatcg tatgtgcatc tttgatattt gagaaataaa agagtacata
     7141 aacgattaca aagattagat ggaaggcacc acgaatcagc ataaagaggg aattatcacg
     7201 acctggtttg ttaccaagag tgatgaggtt gctcaattca ggggttgcaa gtgctaggaa
     7261 gtaaaggaca aatacgatgg ttacaccaag gaagataaaa cctttggctt tttgtttatt
     7321 gtaaatctgt cctaacccag gaatgataga cagcagggct gctttactag gttgttgctt
     7381 ttccatatta actcctttca taggatacta cttttttata agaaagctaa attatttgtg
     7441 tctctattga taaattcaaa gggggatttg atttccaccc cccttgaaca aattttttat
     7501 tcaccaaatt tttgtttgat tgtttctttg atcaatgtta cagcatcgtt agcagctgtt
     7561 ttagcatctt tttgaccact tacagcatca aagagcatat ttttcgctgg atcccaaact
     7621 gcagacattt gagagatgtt tggcagtggt tgagtgttct tgaactgttt gataacagct
     7681 gttgtcaact catcgttttt accttcagcg tatgaacgag cctcagtatt agctgggatt
     7741 tcgttagtct tatcatataa tactttttgt tgttcagttg caacaaggaa gtctacaaat
     7801 ttttgagaag cttcaaggtt cttaacggct tgaggaatga cccaagcttt accaccaccg
     7861 aatgcagcat attcttttcc atttggaaga gttgggatag ttgcaactcc gtagtttact
     7921 ttagcatctt taaaggcttg agctttccaa ggtccgtcga tgatagcagc tgttttacct
     7981 tcttggaatt gagtttggat taagtttcca gcaccttctg tatcttgcat acctttaggc
     8041 catttttcgt accaagattt agcgtaattg atacctacga tagaaccgtc gtttgcaaga
     8101 ccgatgtctt tagcgtcttt accgttttgg ccaaagacgt aagcaccgtt accggcaaga
     8161 agtccatatg tatagtagaa gtttgtccag tcagctagga aggcagtagt tttaccatct
     8221 tcaccagcga atgcgtattt gctatcttta gcaaggtttt ccaagtcagc aaatgttttt
     8281 ggagcatctt tcaccaagtc tttgttgtag tacataacaa gtgactcgat aacggcagga
     8341 gcaccgtaaa ctttaccatt agcagctgtt acaagagatt tagttgtgtc gtctgtttta
     8401 gcaccatcgc tcaatttcac ttctgaaagt tgtccgtcag aaccaaggct acctacacgg
     8461 tcgtatggag ccatcataac atcagggaca ttaccagatt ggttgtcaag agaaagttta
     8521 tcaagacctc ctagagcatc accagtttta agagtgactt ttactccagc ttctttttca
     8581 taagctttag caacctcttc aatatagctc ttatatccct cgtctacata tacagtgagt
     8641 tctttgactt cagatgaacc agaatcagca ggcttatcag cagttttgct tccgcaagct
     8701 accaaaagca agctagcaag tgtagcagtt ccaagcaccg cagtgctctt cataaattta
     8761 gatgacatag tgtattcctc ctaaagaata gcaagtttta ttgataagga aacgcaaacg
     8821 ttttccttta tgagcttagt atagcacaaa tagaaaacgg ttgcaagtat tttttgtaaa
     8881 aattttaaaa aatttttaag cttgatttga tagcataatt ttgcatttta atagaagaaa
     8941 agtatgaaat agataagaat acagtatttt taataaaaat atatggaatc tgtctaaaga
     9001 gggaaatcag gaggggctac ctccctgttt ctaatctata tctattttct atagacgttt
     9061 gtgctttgtt acatataaat tcaagctccc taaggaggtt ttatactttg tagactgtct
     9121 cgtgagagtc aaaccttagg attagatagt tttaagttat ctttgctaaa atgagttagt
     9181 ttccttaaaa ataatcaaaa aagtttttta aaaacgcttg caattatgct tgaaaaggag
     9241 tatacttata agtaacgcaa acgtttgcgt ctgcaaaata cgcaacgttc cattatttta
     9301 acacacgagg tgctattatg aaaaaacgtc aaagtggtgt gttgatgcac atctcttctc
     9361 ttccaggagc ttacggaatc ggatcatttg gtcaaagtgc ttacgacttc gttgatttct
     9421 tggtccgtac aaaacaacgt tactggcaaa tccttccatt aggagcaact agttacgggg
     9481 attctcctta ccaatctttc tcagccttcg caggaaacac tcattttatc gatttagata
     9541 tcttggtgga gcaaggtttg ttggaagcaa gtgaccttga aggagttgac tttggtagcg
     9601 atgcgtctga agttgactat gctaaaatct actatgcacg tcgtcctctt ttagaaaaag
     9661 cggtgaaacg tttctttgaa gtcggagatg ttaaagattt tgagaaattt gctcaagaca
     9721 accaatcatg gcttgagctc tttgctgagt atatggctat caaagagtat tttgacaatc
     9781 ttgcttggac tgaatggcca gatgcagatg ctcgtgctcg taaagcttca gcacttgaaa
     9841 gctatcgtga gcaattggca gacaagttgg tttaccaccg tgtgactcaa tacttcttct
     9901 tccaacaatg gttgaaattg aaagcttacg ctaacgacaa ccacatcgaa atcgttgggg
     9961 acatgccaat ctacgtagcg gaagattcaa gtgatatgtg ggcaaatcca catctcttca
    10021 aaacagatgt caatggtaag gctacttgta tcgcaggatg cccaccagat gagttttctg
    10081 taactggtca gctttggggt aacccaatct atgactggga agcaatggac aaagacggct
    10141 acaaatggtg gattgaacgc ttgcgtgaaa gcttcaaaat ctacgatatc gttcgtatcg
    10201 accacttccg tggcttcgaa tcttactggg aaatccctgc tggttccgat acagcagcac
    10261 ctggtgagtg ggtgaaaggt ccaggctaca agctttttgc agccgttaag gaagaacttg
    10321 gtgagctaaa catcatcgca gaagaccttg gcttcatgac agatgaagtg atcgaattgc
    10381 gtgaacgtac tggcttccca ggaatgaaga ttcttcaatt tgccttcaac ccagaagacg
    10441 aaagcattga tagcccacac ttggcacctg ctaactcagt tatgtacaca ggaacacacg
    10501 ataacaatac ggttcttggt tggtaccgta atgagattga tgatgcgact cgtgagtaca
    10561 tggctcgtta cacgaaccgt aaagaatacg aaacagtggt acacgctatg cttcgtacag
    10621 tattttcatc agttagcttt atggcaattg caactatgca agatttacta gaattggatg
    10681 aggcagctcg tatgaacttc ccatctaccc ttggtggaaa ctggtcttgg cgtatgactg
    10741 aagatcaatt gacaccagct gtcgaggaag gtttgcttga cttgacaaca atttatcgcc
    10801 gaattaatga aaatttggta gatttaaaga aataagacaa taatcaggag acaactaaac
    10861 atgttatcac tacaagaatt tgtacaaaat cgttacaata aaaccattgc agaatgtagc
    10921 aatgaagagc tttaccttgc tcttcttaac tacagcaagc ttgcaagcag ccaaaaacca
    10981 gtcaacactg gtaagaaaaa agtttactac atctcagctg agttcttgat tggtaaactc
    11041 ttgtcaaaca acttgattaa ccttggtctt tacgacgatg ttaaaaaaga acttgcagct
    11101 gcaggtaaag acttgatcga agttgaagaa gttgaattgg aaccatctct tggtaatggt
    11161 ggtttgggac gtttggctgc ctgctttatc gactcaattg ctactcttgg tttgaatggt
    11221 gacggtgttg gtcttaacta ccactttggt cttttccaac aagttcttaa aaacaaccaa
    11281 caagaaacaa ttccaaatgc atggttgaca gagcaaaact ggttggttcg ctcaagccgt
    11341 agctaccaag taccatttgc agactttact ttgacatcaa ctctttacga tattgatgtt
    11401 actggttatg aaacagcgac taaaaaccgc ttgcgtttgt ttgacttgga ttcagttgat
    11461 tcttctatta ttaaagatgg tatcaacttt gacaagacag atatcgctcg caacttaact
    11521 ctcttccttt acccagatga tagtgaccgt caaggtgaat tgctccgtat cttccaacaa
    11581 tacttcatgg tttcaaacgg tgcgcaattg atcatcgacg aagcaatcga aaaaggaagc
    11641 aacttgcatg accttgctga ctacgcagtt gtccaaatca acgatactca cccatcaatg
    11701 gtgattcctg aattgattcg tcttttgact gcacgtggta tcgatcttga cgaagcaatc
    11761 tcaattgttc gtagcatgac tgcctacact aaccacacaa tccttgctga agcgcttgaa
    11821 aaatggcctc ttgaattctt gcaagaagtg gttcctcact tggtaccaat catcgaagaa
    11881 ttggaccgtc gtgtgaaggc agagtacaaa gatccagctg ttcaaatcat cgatgagagc
    11941 ggacgtgttc acatggctca catggatatc cactacggat acagtgttaa cggggttgca
    12001 gcactccata ctgaaatctt gaaaaattct gagttgaaag ccttctacga cctttaccca
    12061 gaaaagttca acaacaaaac aaacggtatc actttccgtc gttggcttat gcatgctaac
    12121 ccaagattgt ctcactactt ggatgagatt cttggagatg gttggcacca tgaagcagat
    12181 gagcttgaaa aactgttgtc ttatgaagac aaagcagctg tcaaagaaaa attggaaagc
    12241 atcaaggctc acaacaaacg taaattggct cgtcacttga aagaacacca aggtgtggaa
    12301 atcaatccaa attctatctt tgatatccaa atcaaacgtc ttcacgagta caaacgccaa
    12361 caaatgaacg ctttgtacgt gatccacaaa taccttgaca tcaaagctgg taacatccct
    12421 gctcgtccaa tcacaatctt ctttggtggt aaagcagctc cagcctacac aatcgctcaa
    12481 gatatcattc acttgatcct ttgcatgtca gaagttattg ctaacgatcc agcagtagct
    12541 ccacacttgc aagtagttat ggttgaaaac tacaacgtta ctgcagcaag cttccttatc
    12601 ccagcatgtg atatctcaga acaaatctca cttgcttcta aagaagcttc aggtactggt
    12661 aacatgaaat tcatgttgaa cggagctttg acacttggta ctatggacgg tgctaacgtg
    12721 gaaatcgctg agttggttgg agaagaaaac atctacatct tcggtgaaga ttcagaaact
    12781 gttatcgacc tttacgcaaa agcagcttac aaatcaagcg aattctacgc tcgtgaagct
    12841 atcaaaccat tggttgactt catcgttagt gatgcagttc ttgcagctgg aaacaaagag
    12901 cgcttggaac gtctttacaa tgaattgatc aacaaagact ggttcatgac tcttcttgac
    12961 ttggaagact acatcaaagt caaagagcaa atgcttgctg actacgaaga ccgtgacgca
    13021 tggttggata aagtcatcgt taacatttct aaagcaggat tcttctcatc tgaccgtaca
    13081 atcgctcagt ataacgaaga catctggcac ttgaactaat actcttcgaa aatctcttca
    13141 aaccacgtca gctttatctg caacctcaaa gcggtgcttt gagcaacctg cggttagctt
    13201 cctagtttac tctttgattt tcattgagta taagatacaa atttatacta atacatcttg
    13261 taaaaaagcg agtttcgatt gaaattcgct tttttaatga tgtagatttg ggtcaatctt
    13321 gtctaaaaat agggaaatcc tagatacagt gaaggcttta aatgctggtt tttactgtcc
    13381 tcagccttat attttttcgt agttgattac ctcatacctc ttgtattcgc ttacataaag
    13441 tattataata taattgtagg aaagaaggtg tttttatgat atacacactt aaattggtgt
    13501 tgtttattac ctttcttgta ataagcttgt tacctgataa gatttttgga aaaaataaaa
    13561 aaatttggaa aatagttttt gcaatattga cggcagtggc agcattgtca tttatgtact
    13621 aagttatttt aagaatgtag ggaaataaac cctacattct ttttagtttt ttctgttttc
    13681 taaattctat ttatccaagc gcttcaacat ttcttgcttc ttcgcttcaa gttctgcacg
    13741 cttttcttcg atttcggcat gttttttctc gagttcagaa caacttgcac cattgctaaa
    13801 ttcttttcgc catcaggaga tagggtgagt cgacatgtct attactcacc caaagcagtc
    13861 ctacaaagca ggaattttct gttacttttt tggaaatagt aacgtttata cagctttgac
    13921 acttcgtatc aaagcgccaa acacactccg agggatttac agaaagcaga aaaggaatga
    13981 tctggtataa gatcattcct tttctctctt tttctttaag taattatata caatgtacga
    14041 cgaagtcgtc attgcaatgc tgatccacca cctaaaggaa actttaaaca acattgataa
    14101 gataaagaat ataaacaacg aaaatacgtt atacccaatt aattttattg tatatctcat
    14161 gattaaaagt taatccttcc gttgttagga atggcatcat ttttatccca taattgtgct
    14221 aaataagtcc ccggtgataa taaatttata gcaaattcta aagcaacatc atttacaaac
    14281 caactaccta gatatctaga aattgctgaa cgaatagcac tttttgctgc atgttttcct
    14341 tttactttaa ttagatttgc aaggcctgca gtagttcctc ctaatgctaa agctattgca
    14401 gtatctaata gagcacccat ttgattaact gtaatacctt gccaagctgc tctaaatgga
    14461 gagtatgtag gtgggattgt ataatcgcct tgtaattgtc ggttaattac ttctttgatc
    14521 cattgttgtg agacgtctgg atgaaaagat tggatttcgt ttgcaagtgt attgatttgt
    14581 tcttctgtta gagaagtgac aggttgaagt tccatatttg tttcaatttg tgatacttgt
    14641 tcagaagcgt atacagctga aacacttgga atcgctgata caattaacac aattgacgtc
    14701 aaaaaaaccg aaataaattt cattaatttg ttcatgagct tttctccttt ttatttgtat
    14761 ctgcttacat tttatcatat gctgttatta tagtcaaaaa aatatgctat tatgttaaaa
    14821 aaatattttt caaaatataa atggacggat ttattttgga ttttatttgt tattttgacc
    14881 tgcctctata ttggtaacca tgatttgttt actctcaatc atcaagaatt ctcttttcgt
    14941 ggtagcgttt ggggtctggt actggcctta tatcacttac tattcattga taagtttgtt
    15001 atatcgaatc gaaaataaag attagagcta tgcttgactg tgtactttta ggattgattt
    15061 tggaggaaga ttttgtctct attatttatt attttaaatt tatttatttt gtataagatc
    15121 tattctttga ggcgggaaaa atcgaaatac ttgatttata cggcctatat catatttggg
    15181 gtaaatgtag tatatggtat tcaatggtta ttaagagaac tgatttcaac tatttcccct
    15241 taatgtgtat ggaaactata acaaaacgca taagattaag gtttgtgtac ctgttcttat
    15301 gcgtttttat aatattgaag attttcggta agttttatac ctttgggaag tctagtttag
    15361 aatgctttcc caaccaaaat ctctgcttcg atggtaatct ctgtcagttg gctccagtca
    15421 atcttgctct ggtcaacgtg aaagaggaga ggagtcatac tgagtagggc ttggagttgt
    15481 tctgctgtga tagtcttagt cagagaggca gtttgactag ataggatggt aaagtgttcc
    15541 tggaaatgct ccttgatatc ttggttagaa tactccttgt ttgtcagctg gtcctgtact
    15601 ctttgacgga tttctttgag gtgattttca gttgggataa cctttatcaa gataccgtct
    15661 ttggataaaa cgcgacgaaa ttctccatag ttggcaggtg agaagatatc aagcagaata
    15721 tccatgttag cgtcttttat aggaagtcgt gccaagtcgc caacgaacca attgactgcc
    15781 cagttgggtt cacttttagc agcgatttgg actgaatctt tggagatgtc aaaggcatag
    15841 aaagtttttt cagagtgact ttcttgtagt ttgcgagaat agaatccttc accacaaccg
    15901 atatccaaaa ttgttgtggt agtttttgag cttgcaagca agtcagatac agcatctaag
    15961 atagcttggt aaaagccggc ttctaggatt tgttgacggt tttgaaaatt ttccttgtca
    16021 tagttagcag attgcttgat ttgaggtgct agattgacat agccaaattt cgccaagtca
    16081 aaagaatgac ggttgcagca cttgaaatta gtctctaaca gagtcagatt ttcttgacag
    16141 ataggacagg caaaggcagt cgcagaagca aaacgctgaa gtttgggctt gagatttgta
    16201 ttcatagttt aagtgtatca aaagaggcaa atgaaagcaa ctttaggaat aagtagattc
    16261 agtagaaata aaactaattc tccaatttat agaatgaaat tgcttttata tctaaattgc
    16321 tatataataa tgataaggag gaaataagta tgttaaaaga agtattaacc gttgcaaaag
    16381 ttgcgaaaaa atcttcatta tttttgggtg gtgtcgcatt tggtaccctt ggtttgaaaa
    16441 tcttagcaag taaggaagct aaaaaaggtt attctaaagc tttggctaag gcttacaagt
    16501 tgaaagacga gctagatgca tctgtttctg ttgtgaagca acatggagac gatgtcttgc
    16561 aagatgccaa atatttgtac gagcaagaga aaaaagaaga gcaattagat agccttatag
    16621 gtgaataata tgtcttttaa agtgctacat agaggatacc aacatatccg actatcatct
    16681 tctttttcac ttaccttgga tattcaagac tatcttcgtt ccttggcgag agatgaaaag
    16741 gggattgagt ctatccagtt ttacatggat caacagcact ttactctacg cataaaagaa
    16801 ggcttttctg tattagataa tgcagaagcc tttttaaaaa gaattgataa agggaaagtt
    16861 tctgagttga tgactcttcc cattcgtaga gaagagagtg cttattctat tgtttcaggt
    16921 gcagcggtta agcgtgtact ttttcgtagt tttgtgccgt atcctattcg ctatatatgg
    16981 acttgttatc aggctttggg ttatattaga gaagcctatc aaacactagc gcgtaaggaa
    17041 ctaacgatgg aagtcttgga ctgttcggct attttattgt ccttgtttat gaaccaatcc
    17101 aagacagcta gcaatatcat gtttatgctt gatttgggga atcatttaga tcagtggtcc
    17161 ttgaaaaaaa ctgcaacaga tttagaacag agtcttcttg caaaagagag cgatgtattc
    17221 ctagtacagg gcgatacggt tgttagtatc aagagttccg atgttcaaat aggagatgtc
    17281 ttgatcttat ctcaaggaaa tgaaattctg tttgatggac aagtagtttc aggtttaggt
    17341 atggtcaacg aaagttcctt gacaggagag agttttccag ttgaaaaaag agagtctgat
    17401 ttggtttgtg caaatacagt attagaaact ggagagttac gcattcgtgt aacagataat
    17461 cagatgaaca gccgtatttt acagctgatt gagttgatga agaaatctga agaaaacaag
    17521 aaaacgaaac aacgctattt catcaagatg gcggataaag tcgtcaaata taatttcttg
    17581 ggggctgggc tgacttacct attgacaggt tctttttcta aggctatttc tttcctattg
    17641 gtcgatttct cctgcgcttt gaaaatctct actcctgtag cttatttgac agttatcaag
    17701 gaagggttga accgtgaaat ggtgattaag gatggagatg ttctggagaa atatctggaa
    17761 gttgatactt tcttgtttga taagacagga acaatcacaa ctagttatcc tatagttgaa
    17821 aaggtgttac cttttggaga ctatagtgag gaagatattc tcagaatcag tgcctgtctt
    17881 gaggaacaca tttatcatcc tattgctaat gccatcgtca agcaagctga gatagaggga
    17941 attgaacatg aggaaatgca tgggaaactc caatatatcg caagcaaggg gatcaaatct
    18001 catatagatg gccaaccagt tcttattggg aattatgtct tgatgcagga tgagcagatt
    18061 catatcagtt cagaacaaaa tgctttaatt gaagagtaca agagtcacta caatctctta
    18121 ttcttggctt atcaaaatga attgattgga atgttctgca ttcatactcc tttgagaaaa
    18181 gaagcaaaaa cagccttgga taaacttaag gcacagggga aaaaattgat tctggcaaca
    18241 ggagacacct tgattaggac agaggaatta gtcaaagatt tgccctttga tcaggtctat
    18301 acagacttga aacctgatgg gaaatttgag ttagtagaga aactgcagaa agcaggtcac
    18361 actattttga tggttggaga tggattgaat gactcagcgg ctttaactct atcagatatc
    18421 ggtgtggtga tgaatgagag tgcagatatt tctaagcaga tgagtgatat cttattgtta
    18481 gataatcgtt tggatttctt ccaagagttg gattcgttat catcatcttt gcaaacactc
    18541 atcaagaaaa atattcagga taccgttgtc gtaaatagta gtttgattgg ctttggctta
    18601 tttaattggt tcagtccttc aaatctctct atcttacata atctaacaac cttgcgcatt
    18661 gtcctgcgta gcctgtctat taaaaataga tagatgagag ttattaacag caaaaaggag
    18721 ttacctttct cgaggttaac tcctttgttt atagataaat gttgaatagt ttagtaagtc
    18781 aatacaaagt attttttctt cccacgacgg ataacagtca gttcattttc taacttatca
    18841 gcgtcactca agacatagtc aaggtcttgg atgcggtcgc cgtttacgta gatggctccg
    18901 ttttggacgt cttcacgggc ttggcgtttt gagttaacta taccagatga gacgagcagt
    18961 tccacgatat tgttgttttc gtctgcctgt acttggtagt ttggcacacc acgaagtcct
    19021 tgtttgagct ctttgacaga aaggtttttg atgtttcctg caaagagttg ctcagtgatg
    19081 ttaagtgctt ctttgtaggc ttcttctccg tgaacaagtg taacaacttc acgagccaag
    19141 actttttgag ccaagcgttc gtgtggcgct gcttcaaatt gtttacgaat atcttcaatc
    19201 tcatcaagtg acaagaaagt aaagattttc aagaagcgaa cagcgtcagc gtccatcacg
    19261 ttcatccaga attggtacat ttcgtatgga gaagtctttt cgggattgag ccagacggca
    19321 tttccttctg atttaccaaa ttttttacca gttgcatctg tgattagtgg aacagtgata
    19381 acgtgaccag tcttgtccgc cttacgacga agcaattcgg taccagctgt catatttccc
    19441 cactggtcag aaccaccgat ttgaagagtg acattatggt cttggttaag gacgaagaag
    19501 tcgtaccctt gcatgatttg gtaagcgaac tcagtgtaag aaattcctgt ttcgatccgt
    19561 tttttaacag attccttact catcatgtag ttgaccgtga agtactttcc aatgtcacgg
    19621 aggaagtcaa tgaagctgat gctgccaaac cagtcgtagt tgttgaccat gacagccttg
    19681 ttttcgccat tttcaaagtc aagaaaacga gaaagttgtc cttggataga cttgacccag
    19741 ccatctactg tgtcttttgt ttggagacta cgttcagcat ctttgaagga cggatctccg
    19801 atgagacctg tagcaccgcc aacgagcgca taaggtttgt gacctgctag ttgcaagcga
    19861 cgacttgtca agattgcgac aaggtggcct aggtgaaggc tgtcagcagt tggatcgtag
    19921 ccagtataat aagaaacttg accttcttct agggctttac gcaaagcttc ttcatcagtc
    19981 gtttgaaata tcaaaccacg ctcttttagc tcatcaaaaa tgtgcatgtg tcttttctcc
    20041 tttttaaaat tattgtttct acctattgta tcacaaactt gggcaatagc ctagcagaat
    20101 agggaagaaa gtggctattt tattgaaaat ttcccttttg tggtataata gataaagtga
    20161 ggaaatccat gcaaaatcaa ttaaatgaat taaaacgaaa aatgctggaa tttttccagc
    20221 aaaaacaaaa aaataaaaaa tcagctagac ctggcaagaa aggttcaagt accaaaaaat
    20281 ctaaaacctt agataagtca gccattttcc cagctatttt actgagtata aaagccttat
    20341 ttaacttact ctttgtactc ggttttctag gaggaatgtt gggagttggg attgctttgg
    20401 ggtacggagt ggccttattt gacaaggttc gggtgcctca gacagaagaa ttggtgaatc
    20461 aggtcaagga catctcttct atttcagaga ttacctattc ggacgggacg gtgattgctt
    20521 ccatagagag tgatttgttg cgcacttcta tctcatctga gcaaatttcg gaaaatctga
    20581 agaaggctat cattgcgaca gaagatgaac actttaaaga acataagggt gtagtaccca
    20641 aggcggtgat tcgtgcgacc ttggggaaat ttgtaggttt gggttcctct agtgggggtt
    20701 caaccttgac ccagcaacta attaaacagc aggttgttgg ggatgcgccg accttggctc
    20761 gtaaggcggc agagattgtg gatgctcttg ccttggaacg cgccatgaat aaagatgaga
    20821 ttttaacgac ctatctcaat gtggctccct ttggccgaaa taataaggga cagaatattg
    20881 caggggctcg gcaagcagct gagggaattt tcggtgtaga tgccagtcag ttgactgttc
    20941 ctcaagcagc atttttagca ggacttccac agagtcccat tacttactct ccttatgaaa
    21001 atactgggga gttgaagagt gatgaagacc tagaaattgg cttaagacgg gctaaggcag
    21061 ttctttacag tatgtatcgt acaggtgcat taagcaaaga cgagtattct cagtacaagg
    21121 attatgacct taaacaggac tttttaccat cgggcacggt tacaggaatt tcacgagact
    21181 atttatactt tacaactttg gcagaagctc aagaacgtat gtatgactat ctagctcaga
    21241 gagacaatgt ctccgctaag gagttgaaaa atgaggcaac tcagaagttt tatcgagatt
    21301 tggcagccaa ggaaattgaa aatggtggtt ataagattac tactaccata gatcagaaaa
    21361 ttcattctgc catgcaaagt gcggttgctg attatggcta tcttttagac gatggaacag
    21421 gtcgtgtaga agtagggaat gtcttgatgg ataaccaaac aggtgctatt ctaggctttg
    21481 taggtggtcg taattatcaa gaaaatcaaa ataatcatgc ctttgataca aaacgttcgc
    21541 cagcttctac taccaagccc ttgctggcct acggtattgc tattgaccag ggcttgatgg
    21601 gaagtgaaac gattctatct aactatccaa caaactttgc taatggcaat ccgattatgt
    21661 atgctaatag caagggaaca ggaatgatga ccttgggaga agctctgaac tattcatgga
    21721 atatccctgc ttactggacc tatcgtatgc tccgtgaaaa gggtgttgat gtcaagggtt
    21781 atatggaaaa gatgggttac gagattcctg agtacggtat tgagagcttg ccaatgggtg
    21841 gtggtattga agtcacagtt gcccagcata ccaatggcta tcagacctta gctaataatg
    21901 gagtttatca tcagaagcat gtgatttcaa agattgaagc agcagatggt agagtggtgt
    21961 atgagtatca ggataaaccg gttcaagtct attcaaaagc tactgcgacg attatgcagg
    22021 gattgctacg agaagttcta tcctctcgtg tgacaacaac cttcaagtct aacctgactt
    22081 ctttaaatcc tactctggct aatgcagatt ggattgggaa gactggtaca accaaccaag
    22141 acgaaaatat gtggctcatg ctttcgacac ctagattaac cctaggtggc tggattgggc
    22201 atgatgataa tcattcattg tcacgtagag caggttattc taataactct aattacatgg
    22261 ctcatctggt aaatgcgatt cagcaagctt ccccaagcat ttgggggaac gagcgctttg
    22321 ctttagatcc tagtgtagtg aaatcggaag tcttgaaatc aacaggtcaa aaaccaggga
    22381 aggtttctgt tgaaggaaaa gaggtagagg tcacaggttc gactgttacc agctattggg
    22441 ctaataagtc aggagcgcca gcgacaagtt atcgctttgc tattggcgga agtgatgcgg
    22501 attatcagaa tgcttggtct agtattgtgg ggagtctacc aactccatcc agctccagca
    22561 gttcaagtag tagttctagc gatagcagta actcaagtac tacacgacct tcttcttcaa
    22621 gggcgagacg ataattctct aaatgaagtg gccaatcaat ggattgccac ttttttcctt
    22681 tcgtgttaca ataagggtat gaatcagtat cagaaaaaga ttgttaacgg aaaaatttat
    22741 tcgctcctat ccggcttaat atggggaatc tgtggaattt taggagagta cttctttact
    22801 cattatcagg tgtcttcggg ctggattacc tctatgcgtt tgacactggc agggagtctt
    22861 gtactcattt ggtctgcaat acaattaaaa tcgcaagtgc tagatatttg gcgagacaag
    22921 aaaaattacc tgcccttttt agcctatgct attttgggga ttttttcagt tcagtatttt
    22981 ttctatctct gtttagaata ctcaaatgct acgacagcaa ctattttaca gtttattagc
    23041 cctgtcttta tcctctttta caatcgcttg gtttatcaaa aacgagcgtc aaaaagcgct
    23101 gttttctatg ttttggttgc catgctgggt gtttgcttga tggcgacaaa gggagatctc
    23161 tctcagttat ccatgacgcc gctagctctt ataacaggtt tgctgagtgc catgggtgtt
    23221 atgtttaatg ttatcttgcc ccaacctttt gctaagcgtt atggttttgt tcctacggtt
    23281 gggtggggga tgattttggc aggtttgttt agcaatgtcc tctcgccggt ttatcagctt
    23341 tcctttactc ttgatatttg gagtatcttg atttgcctca ttatcgcttt ctttggaacg
    23401 gcttttgctt ttttcatttc catgaaggct gtgtccttgg tttctccttt ggtggtttcc
    23461 gttatcagtg ccagtgaacc tctctcttct gctctcttga gtgttttgtt cttaggatta
    23521 gtagtggatt ggtccctcct tctagctata gccttgatta ttttacccat gatatttttg
    23581 tctatagaag aagcgaaaga aagtagataa aaagtctttt cttaattcta aaagtgtgct
    23641 atactagaat agtcaataaa acacggggag atagttgtga agagtatttt taggttgtat
    23701 cggtaggggc ttgcttttac cgacagcacg ttttatctca catccctaaa aaatcatacc
    23761 taaaaacaga caaaaaggtt tcaaatttga ggcctttttt ggtagaatag gtatcattat
    23821 aacgaaccag gaggcaccta tgactgctac aaaaatgaac gctcaagaaa ttatccaatt
    23881 tatcgccaat gctgaaaaga aaaccagtgt caaagtaacc tttgaggggc aactcgcaac
    23941 tgctgtgcct agctctgttg tcaaactagg aaatgtccta ttcggagact ggaaggacgt
    24001 ggctccgctt cttgaaggtt tggtagaaaa tcaagattat gttgtcgagc aagatgctcg
    24061 taattctgca gttcctttgc tagataagcg tgctatcaac gctcgtatcg agccaggtgc
    24121 tattatccgt gaccaggtgg aaattggtga caatgctgtt atcatgatgg gagctgttat
    24181 caatatcggt gctgaaatcg gtgctggaac catgattgac atgggtgcca tccttggtgg
    24241 ccgtgctatc gttggaaaaa atagccacgt tggtgcaggt gcagttttgg caggtgtgat
    24301 tgagccagct agtgctgaac cagtctgtgt cggagacaat gttcttatcg gggctaatgc
    24361 agtggttatc gaaggagtcc aaatcggtag tggttcagtt gtcgctgcag gagctatcgt
    24421 tacccaagat gtcccagaaa acgtggtagt agcaggtgtt ccagctcgta ttatcaaaga
    24481 aattgatgcc caaactcaac aaaaaacagc gctagaggat gcgcttcgta ccttgtaatt
    24541 gtaaaagtaa aaaagaggcg gaaccctttt tccagcctct ttctgctata tcggaggaca
    24601 gatagatgtt agatttgatt cagactagac gagctttaca ccagattcca gagattggct
    24661 tggaggagtt caagactcag gcttatttgc tggatgtgat tgagaaattg actacgggca
    24721 aggattttgt tcaaattcgt acttggcgga cagggatttt ggtctacctg cagggaagtc
    24781 agccggagcg aaccattggt tggcggacag atattgatgg cctgcctatc gtcgaacaaa
    24841 caggtctacc ttttacttct caacaccaag gtcgcatgca tgcctgtggt catgattttc
    24901 atatgaccat tgccttaggc tgtcttgagc gcgcccttga ggagcaaccc aagaataatc
    24961 tgctctttct atttcagcct gctgaagaaa atgaagctgg tggtatgctc atgtatgagg
    25021 acggtgcttt tggagattgg ttgccaaacc aattctatgg tctccatgtt cgtccagatt
    25081 tgaaagtcgg ccagattgcg actaatactc atacactctt tgcaggaact tgcgaggtga
    25141 agatccgttt caaaggtaaa ggtggccacg cagcttttcc tcatgaagcc aatgacgcct
    25201 tggtggcggc tagttacttt gtgacccaag tgcagtcagt tgttagccgc aatgtcaacc
    25261 caatcgaggg agcagtggtg accttcggcg tttttcaagc tggaaccacc aacaatgtca
    25321 tcacagacac agctttttta catggaacta ttcgtgcctt gacacaggac atgagtctct
    25381 tggtgcaaaa gagggtaaaa acagttgcag aaggggttgc agctgccttt gatatggaag
    25441 tcgaagtgga actcaaacaa ggtggatacc tacctgttga gaacaatcca gccttggcgc
    25501 gtgaactgat ggacttcttt gacgagaaag acggaatcga gttgattgat atcgaacctg
    25561 ctatgacagg cgaggacttt ggttatctcc tttcaaaggt tgatggcgtg atgttttggc
    25621 taggtatcga tagtccctac gctcttcatc accctcaaat gagtcctaag gaagaagtct
    25681 tagctattgg ggtggctgcg gtgtctagtt tcctgaaaaa gaaggcagca gaatagaggg
    25741 cttgtctatg aaatcggaat tacgcaagca agtcttgcat gaaatgaagg ctatatctca
    25801 agagcaaaaa caggctatag accaagcttt aaccgagcga attttacaac acccctttta
    25861 tcaagaagcc aaggtcatcg caacctacct ctctttttct catgagtttc aaacgcggga
    25921 actgattgag caggcgctga aggacggcaa gaaggttttg acacctaaga cctatcccaa
    25981 ggggcgcatg gattttgtgg tctatgatcc gcaacagttg gtaaaaactt cctttgcctt
    26041 actggagcca cagggagatt tggaagtagt ggatgcatct cagattgatt tgattcatgt
    26101 tcctggcctg gcttttacga cggatggata tcggattgga tatggtggag gttattatga
    26161 ccgctatctg gaacattttt ctggtcgtgc tttgagtacg atatatcctt gtcaaattca
    26221 ggactttata cctgaaaacc atgatattcc tgttcaggag gtattaattg atgaaggaaa
    26281 tctttgatag acgttaccct gtgacgagtt tcttcctctt agtgacggcc ttggtatttt
    26341 tactaatgtt ggtcactgca ggcggaaact ttgacagggc agatacatta tttcgatttg
    26401 gagccatgta tgggccagct attcgcctct ttcccgagca ggtttggcgt ctcttgtctg
    26461 ccatttttgt tcatattggg tgggaacatt tcattgttaa tatgctttca ctttattatc
    26521 ttggaaggca ggtagaggag attttcggtt ctaagcagtt tttctttctc tatcttttat
    26581 caggaatgat gggcaatctc tttgtttttg tatttagtcc taaatcctta gcagcaggcg
    26641 cctctacctc tctttatggg ctatttgccg cgattattgt tcttcgctat gcaactcgca
    26701 atccttatat tcaacagcta gggcaatcct atctgacact ttttgtagtt aacattattg
    26761 gaagtgttct gattccagga atcagcctag caggccatat cggtggtgca gttggtggcg
    26821 catttctagc agttatcttt ccagttagag gagaaaaacg gatgtataat accagccaga
    26881 gattaggagc ggtagtcttg ttcgtaggac tcgccatttt gcttttctac aagggaatgg
    26941 gaatgtgacg aagttgaaag aaaatttgtg taatgaaaaa agataaggca ttttttgaac
    27001 cttatctttt tattttattt tattccttct cagtcaattc ttttccaagt tggatgaggt
    27061 aattcttcaa atcatctttg acttgtgggt gtttgagggc gtagtcgatg gatgttttca
    27121 tgaagccaaa cttgtctccg acatcgtaac gagccccatt gaactcacga gcaaatacac
    27181 gttgtgtttt attgagggtg tcgattgcat ctgtcagctg aatttcattt cctgcacctg
    27241 gagcttggtt ttcgaggatt tggaaaattt caggcgtgag gaggtagcgt ccgataatag
    27301 caaggtcgct aggagcgtcc tctggagctg gtttttcaac aaaggtttca acactgtaaa
    27361 gaccatcttt tccttcgcct tgcggagcaa taaccccgta agcagatact tcgtcatgag
    27421 ggactggcat gacagcgata gtagacgcgt gggtacgctc gtagtcatcc atgagttgtt
    27481 tggtaagtgg aacagccttt tcgtctgtga tatccatcaa gtcatcacca agcataacga
    27541 caaaaggttc atttccgacg aaagccttag cttgcaaaac agcatctccg agaccgcgtg
    27601 gatgagtttg gcggataaaa tgcagacgca tgtcagttgt tttatcaact agcttcaaaa
    27661 gatctgtttt ccctttttct ttgaggttat attccaattc gaagtttgaa tcaaagtggt
    27721 cctcaataga acgttttgat ttaccagtga caactagaat atcttcaata cctgatttga
    27781 gagcctcttc cacgataaac tggatagttg gtttgtctac gattggcaac atttctttgg
    27841 caagggcctt ggttgctggt aaaaatcgag ttcctagtcc agcagcaggg atgactgcct
    27901 ttctaacttt tgatgtcata aaaatccttt ctatagaggg ttaagaccac tcattttctg
    27961 ctttaaattc attgttcatg atgtcataaa tggcatcttt gatattggtt ccgtgataaa
    28021 taacttggta aatagcctgt gtaatgggca tatagactcc aagttcttgg gctagttcat
    28081 aggctgctcg agtcgttgaa attccttcga ttaccatgcc catattagct tctatatcag
    28141 ctagggattc tcctcgtccg agagcatctc cagctctcca gttacgagag tggatggaag
    28201 ttcccgttac gatcaaatct cccacaccag ataagccgct ataggtcaat ggactggccc
    28261 cgagtgctac ccctaggcgg gtgatttctg ctaaacctcg agcgatgatg gctgccttag
    28321 cattatcacc aaatccaaga ccatgtaaag ctccagcacc gacagcaata atatttttaa
    28381 gagcaccagc agtttcaacc ccgataacat ccgtattggt ataaagtcgg aagtagtgat
    28441 tactaaatag ctcctgaacg tattgagctg tttgtaaatc tttagaagca gcagtgatta
    28501 aagttaggtc acgcacaatg gtttcttccg catgactagg ccctgaaaca acgacaatat
    28561 cactgcggag ctgttcagga atttcttctt caaggatagt tgataatcgt ttatggctat
    28621 caggttctaa tccctttgaa gcgtgcatga tgatagcctt atggtccaag gtttgtgcaa
    28681 cttgttgggc aacaagtcgt gtcacttttg ttgggacaac aaacaaaatc gcatccacat
    28741 ctttcaatgt ttctgctaag tcggtgtagg caatgatatt ttcatctaga acgacatctt
    28801 taaagtagtg cttgttagta tgataggtat taatttcatt gatttgctcg ggaagatttc
    28861 cccaaatacg tacctcgtgt ccattgtcat ttaagacttg tgaaagggca gttccccaag
    28921 aaccaggccc caagacggcg acggtttgtt tttccatgat ttcctccttg atagagttct
    28981 ttccattatt ctatcatatt ctagaggatt ttgcacttgc attactgggg tatggtggaa
    29041 aaatacacaa agaccgagat gaagggataa actcttagtc tttagttaaa gtgagaagaa
    29101 atctgataga tagtttgatt cagtatatgc tataaataga ggtaaacaac tcaggaggtt
    29161 cttgtaggtt gcgagagttt ggcgaaaaaa ttaaaagatt acgtttggct aaaaaaatca
    29221 gtcgttcaga attttgtggt gatgagtctg aattaagtat ccgtcaatta attagaattg
    29281 aaaatggaga atccagacca acactaacaa agttaaaata tatcgctgaa cgtttggggg
    29341 gggtgaagat tacaagttga tgccaagtta tatagagttg gataaggaat acctagaatt
    29401 gaagtatttc ttgatgagga ctcctacata cgaagatgaa actatcgccc aaaagaaaga
    29461 gagtgttttt gctaagattt ttgaagagta ttatgatagg ctacctgagg aagaaagatt
    29521 tatcatccca aattattcat atctagcact agcgaactac acagttcaaa aattaccaga
    29581 aaagctagtt gaaatactgt ccttctggtg attttgctta atttctgatg ttcgagacat
    29641 gattgaggag atttgtcttg attttttcct aaaaaagggc agactcctcc cttggtttga
    29701 tgctcgattt ttaaaataag gtgacttatt ctgttaggca tgttttcccc tgccctctag
    29761 ctctatatgg aacagggagt atcagattta tctggcagat tgtatcaaat aaggcttgaa
    29821 actgttttga ataggcgtat agacttgaga atttgagaat atgtcgtcta gcagtagaga
    29881 catattttcc ggcaatatga aggaagagac gtcgaaaacg cttgatagtc aaggacttta
    29941 cttcatcacc agctagctgc tttaaaaaga ggtagagatt ataagccaga cagcccatca
    30001 tcatacgtac ttcattctta atcatggtcg aactatctgt cttatcccca aagaatcctg
    30061 ctttcctttc tttgataaag ttttcagcat tccctcgttc acgatagaat tgaaacagct
    30121 cctcactatc tacactgctt aaattgttca aatgaacttt tttacaacca taaaaaagag
    30181 cacttaatcg tactcttctc ttttaaggat tagttgaatt aattcaaatc cactagttct
    30241 cctgtttcta ggtagactag gcgttcacaa atgttagcaa tgtaatcagc gaagcgttcc
    30301 agatgtccta tgatataaag gtattgagtt ccattgggaa ttgaggtttc ttggtctttc
    30361 ataagtccaa tgatttcctt tgataaggca taataatatt ggtcaatctg ttcatctttt
    30421 tgagcaatac taatagcttt tgaggcttgg tgcaaaggaa aggcaaccaa taaatcggct
    30481 agcatgctga gggataattt acccatttgg tgtaactgtt cttcgtcagg ggctagttga
    30541 ttttctttta gttgcaaaac agctttggca atgcctgcca tatggtctcc catacgttca
    30601 aggtctgaac aagaagacat gatgctaatc acaaatcgaa ggtcagacac ttgtggctgt
    30661 tgcaaggcca acaaacgggc acaggtcaat tcgatagcgc tttgaccttg gttgatagca
    30721 tgatccttat tgataattag ctctgccatc tccttgtctt tggaggctaa ggccagtaag
    30781 gcttttgaag ctgtttcaag gacaagttgc cctagtccta aaaaggattg ttctaattca
    30841 tgcaattcta agtcaaattg atttctcata ggtttttcct tccttatccg aaccgtcctg
    30901 aaatatagtc ttctgtgcgc tgatcttttg gattggtaaa cacgtcaacg gtatctccaa
    30961 attcgcaaat ttctcctgtt aagaaaaaag cagttttatc tgaaatacgt gaagcttgtt
    31021 gcatgttatg ggtaacaatg ataatcgtat aatccttttt tagttgctga atgaggtctt
    31081 caatttttaa agtggagata gggtctaagg ctgaagtcgg ctcatccatt aacagaacat
    31141 caggttctac tgctaaagct cgcgcaatgc aaaggcgttg ctgctgaccg ccagataagg
    31201 acatggcact ctttttaaga tcatctttga cttcttccca aatggctgcc ccttttaaag
    31261 atttctccac taaggcatct aattgttttt tgtctcgaat accatgtgtc cttggaccat
    31321 aagccacgtt atcatagata gacatggcaa agggattagg ctgttgaaaa accatcccta
    31381 cacgcttgcg tagctgatta aggttgaatt tgctactata aatatcttgc tcatctaaga
    31441 ggacttggcc ttcaatatgg caagaaggaa ccaaatcgtt catccggtta agggttttta
    31501 gaaaagttga tttgccacaa ccagatgggc ctatcaaggc agtaatctgt ctttctggta
    31561 attgaatcga aatatttttt aaggcttgaa aatccccgta aaataagtct aggtgtctga
    31621 ctgaaaatgt tcccatacta ggaagctcct ttcacaagtt ttcgagataa taagcttgat
    31681 agagtattta tcattaaaac agtaataatc aaaatcacac cggtagcata ggcttcattg
    31741 acatgtagcc cctcacttga cagcatatac atatgcaggg ctagagaacg acctgaagac
    31801 atgagactac ttggcgtatt ggtagaggta cctaatgtat acatgagggc agctgtctca
    31861 ccaacgatac ggccaatagc tagtatcact ccagctaaaa tacctggcat ggcaactggt
    31921 agaacaattc taaaaacagt ccgtaactta cctgccccaa gtccataact tgcttgacgc
    31981 atgctatcac taacagataa aagggcttct tctgttgagc gaataatgac tggcaacacc
    32041 atgatgactg aggttaagat tcctgataac agagagtatt gaaaacctaa gaagactaca
    32101 aagaagagca tgccaaacag accaaaaaca atggaaggaa tcccagacaa ggtatctgag
    32161 gccaatcgca tgattttaac acaaagggaa tctttttttg tatattccac aagataaaaa
    32221 ccagcaaaaa tccctatggg caaggctaaa agaagagcac caaagaccag aataacggtg
    32281 gaaataatcg ctggcataag ggaaatgttc tcagaagtat aagtccaaga aaagagggat
    32341 agacttagat gaggtaagcc tttgatgagg ataaaaccaa tgattaaaaa gagagagcca
    32401 aaggttaaag ctgaaaaaca ataaacgaga agttttagca ggtatttact cataagatga
    32461 ttttcctttc aagtaggcaa agtaggcatt aatcaagaga ataaggaaaa agagaactgc
    32521 tgaggttgca ataagggctt ccctatgctg acctgatgcg taagccattt ccagaacaat
    32581 attggttgtt aaggttctgg ttcctgaaaa gagtccactt ggaataatcg gctggttgcc
    32641 tgccaccaaa ataactgcca tggtttcacc tactgcgcga ccgattccta aaataactgc
    32701 tgataaaata ccagatctcg cagctggcaa gatgacacta aaaatactcc gttcatgact
    32761 agctcctaga gccaagctac cagaataata cgttttggga actgttcgga tagcagattc
    32821 tgacaaactg ataatggttg gcaaaatcat tattcctaat agtaacgaag cggttaggac
    32881 actcatgcca tttcctaaaa aggttctaat ccaaggcacc aataattgta ggccgaaaaa
    32941 accataaaca atagatggaa tggccgccat caagttgata gctgatttta agaagccata
    33001 gacgggcttt ggacaataat aaaccataaa caccgatgtc aagatgcctg ttggcacccc
    33061 aatcacaatc gctcctaagg taattaataa ggaaccaacg atcattggta aaataccata
    33121 gcttgccgga atgttcgttg gcgaccaatc actgcctaat aaaaaacggg caaagccgta
    33181 gttagccatg aaaggtaagc cattactaaa aataaagaaa cagattagca aaatagctac
    33241 aacagctact gttgcactca tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg
    33301 ttttgtcaca tcttgtcctt tctagtgaag aaagtaaggg agatacgaca cctccctact
    33361 tgccttcttt atcttattgt acgatgaaac gtctgcatct ctttagagat ttatggagca
    33421 aacattttat ttaatcttgt cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc
    33481 agccatactg acttggcttg ccttattgtc attattgacc acaacagcaa taccgtctaa
    33541 agcaatagca tcatgggtga gactcttacc ttcttcagga gttaattccc tagaaaccat
    33601 accaatatca gcggttttct ccttaacagc ggtaatacct gctgaagacc cattagaggt
    33661 aatatcaatc gtaacttctg gattttcttt tttataagct tctgctaatt tttccattaa
    33721 agaagatact gaagtggaac ctacaacaga caacttgcct gataagtgtt ggcttgtata
    33781 ttctgtggtt tcggttttag cttcaataaa tttattatct gtgaccactt gttgaccttg
    33841 tttggagtgg ataaagctga taaaatcttg acctagcttg gaaagattag aagaccaaac
    33901 aatgttgaag ggacgttgaa gagggtattc accatctaaa actgtgtctc gactagcctt
    33961 gacaccatca atctctaaag ccttgacaga tttcgttaaa gatcccaagg agatgtagcc
    34021 gatagcatta gcattccctt gaactgctga gagaacacct tctgtactat tttgaatcac
    34081 agctgttttg gcagtgttgt caattttttt atcaccgtct tttttgagaa tccctgtgat
    34141 ttctgtgaag gcaccccgtg ttccagagcc attttctcgt gaaatcacct caatcgttcc
    34201 tgaagctgac tgtttggaag cagctgactg attgccacag gcaacaagcc caaatcctga
    34261 taagccaatg gctgcaagag taagcatttt tttgaatttc ataataatca cctttatctc
    34321 tatgtatttt tcttgtgtag gcttactaca tttatagtct aacaagtctt tgtaaaggtt
    34381 tatccctgat tcatgtaaag attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg
    34441 ctgctcctaa aaatagcttt aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa
    34501 aaatcgtgaa gcaactgcct ctgccaagct gactcgtcac cgtgacttgg ccacctaata
    34561 attgactgag ttctttgaca atggcaaggc caagaccagt gccaccagtt tgtctgcttc
    34621 gacctttatt aactcggtaa aaacgttcaa aaatacgatc ctgctctaat tgactaatac
    34681 caatccctgt atctgataca gaaatcttaa tgccttcgtt caccttttgg gtcttgacct
    34741 caatttttcc cccttgttca gtgtaacgga tggcattgga taaaagattg agtaagattt
    34801 gggaaagtaa ttgactatct gatacgaggg tgacatcatc tggcacctgc acctttagct
    34861 gtaaatcctt cttcttgagc tgaggttgca agctttgagt caaatcctgt acaaattctg
    34921 ccaaagaaag ggtcgtccat tgtataggca tttgttgagc cttagataag gtaagaagat
    34981 gctcaacaat atgctcaaga cgcaagcttt ctttgtaaat aatgtctaga aagtcatcct
    35041 tgagcgcttc ttcttcagct gacatcccct taatggtttc agcaaagccc ttaatcgaag
    35101 taactggtgt cctcaattca tgggaggcat ttgagacaaa ggctaaattt aacttttcat
    35161 aagttctaat cgttgttaaa tcatatagca agacgagcac agcttccaca gattgggtgg
    35221 ggctaaaaac gggaactgct gtcacttcta aaatcaagtc accctcatga aacccactta
    35281 cttcttgttt taaccttgtt ttttgatcaa aggcttggtg aactaaattt cgaatatcca
    35341 tccgtttgag gtcatcaagt gaacttatgt cgccgtccac atcgggaaaa taatgaggca
    35401 gagagcgact ggataataac atctgacctt gagcggaaac taaaaacgtc cccatggtta
    35461 ggtgcgacag aagaacctcc attgtttcgg ctaggtcctt gtattgctga tcctgttggg
    35521 agactttggt ttttagggca gacacatact gagccaaaga ctttaagtct tcttgccctt
    35581 tttctaaaaa gtattcacta ctggtcaaga gaggttggtg caaggtctca aaagcaactt
    35641 cccatttcca aaggcaaaag agccagtagc caccttgtcc caaagaaagg gctagaagaa
    35701 agagaccgat gcctttactg atccaagtta atgccatccc tgcaatcaga atgaggctaa
    35761 cacttagatt gactagccaa aattgaaggt agcgtttcat ctataactcc ttgaacttat
    35821 aaccataacc ccgaatggtt cgaataaatt gaggggcttt aggattgtct tcaatttttt
    35881 ccctcaactt accaatatga acgtccacca aacgtgtttc ctgcccaaag tcataccccc
    35941 agatacgttc caaaagacgc tctctagtca gtgtcatgtt gggatgtttc ataagataga
    36001 gcaagagttc aaattctttt ggggtcaaac tcagtaactt attcgccttg tagacttcat
    36061 gacgctcagg gtatactttc aaggtcccaa atagccaaga atcgtcagcg atattatctg
    36121 aatcatctcc ttcttgttct cctttagttc gcctgaggac agccttgaca cgcgccagca
    36181 attctctagg gctaaaaggc ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa
    36241 ccttatcaaa ttcatcactt ttcgcagaaa ccatcataat tggagttttg acgcctttgg
    36301 ctctcagccg cttacaaact tccatgccat ctaattgtgg taacatgata tcaagcaaga
    36361 taaaatcaaa gggttctgtt tctgccaaag ctaaggcctt ccgtccattt gttaccaatt
    36421 gagtagaaaa gccttcctta cttaaatggt agtcaagcaa tttcagaatg tgttcttcat
    36481 catccactaa taagacttgt tttgtcatct attatctcct attggtaaca ttataacaca
    36541 attatcagaa atcctaacat tgctaaatca gattaaattt gcttatcaag actagtatct
    36601 ggtcaaacgc tcaatcatct ccttgtgctc tggataggtc gccagtagat ctaccctttc
    36661 aaataattca aaatcctcaa attcaaaacc aggagcaaca agacaagaaa ccagagcata
    36721 atccttatca actgttgatc cccaaatagt gcccttagga acacagtagt gaagttgttg
    36781 ccctttggat atgtccaggc ctaaagtgac tgcttcgtag tgaccatctg ctgtaatcat
    36841 gtgaacagta agtggggatc ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg
    36901 aaaatgtgaa ggattcgttt cttctaataa gaaataaata ctggtataaa gcgcccttcc
    36961 cttaccagca aggtttatag tgtctgaagc tttttttgtt tgtctaaaat agccaccttc
    37021 aacatgggga gctaactcta gagttcttat caagtcttct ttatccgtcg gagccaatgg
    37081 gttgaagtaa ctcttgttca aaggtggtgt tacgatttca agaactcctc tcagctctga
    37141 ggacacgata atgattgatg cgacggaagt acaaatcaat cgccctaaaa aaagaattag
    37201 cgaattattc tggtaaaaaa atgccacgc
//
LOCUS       DAVBCB010000016        37031 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVBCB010000016 DAVBCB010000000
VERSION     DAVBCB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.016"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            150..488
                     /locus_tag="VQU28_000773"
     CDS             150..488
                     /locus_tag="VQU28_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HET2476154.1"
                     /translation="MTLTIHVYYKGDGDCAIKFAREMLESGLVAEIRCQKGNLKYEYF
                     LPIEKEGIILLIDQWINQEALDEHHQSNTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            678..2114
                     /locus_tag="VQU28_000774"
     CDS             678..2114
                     /locus_tag="VQU28_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2476155.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKKVILSNGESVE"
     gene            2166..2405
                     /locus_tag="VQU28_000775"
     CDS             2166..2405
                     /locus_tag="VQU28_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HET2476156.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2535..2849
                     /locus_tag="VQU28_000776"
     CDS             2535..2849
                     /locus_tag="VQU28_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HET2476157.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            2967..4946
                     /locus_tag="VQU28_000777"
     CDS             2967..4946
                     /locus_tag="VQU28_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2476158.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFAQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLFKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNSFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQPFGTEGKVSVAICKDSLLWDNQSSYVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFISIFEKIK"
     gene            4956..5270
                     /locus_tag="VQU28_000778"
     CDS             4956..5270
                     /locus_tag="VQU28_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HET2476159.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            5301..5807
                     /locus_tag="VQU28_000779"
     CDS             5301..5807
                     /locus_tag="VQU28_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476160.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFINPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQILISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            5887..7233
                     /gene="celB"
                     /locus_tag="VQU28_000780"
     CDS             5887..7233
                     /gene="celB"
                     /locus_tag="VQU28_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HET2476161.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            7613..7789
                     /locus_tag="VQU28_000781"
     CDS             7613..7789
                     /locus_tag="VQU28_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476162.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            8199..10412
                     /locus_tag="VQU28_000782"
     CDS             8199..10412
                     /locus_tag="VQU28_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HET2476163.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHFFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(10634..11110)
                     /locus_tag="VQU28_000783"
     CDS             complement(10634..11110)
                     /locus_tag="VQU28_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HET2476164.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRIEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            11333..14533
                     /locus_tag="VQU28_000784"
     CDS             11333..14533
                     /locus_tag="VQU28_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HET2476165.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAAVHRMVPIEAKKKYKLRFKIKTDHKVGIAKVRIIEESDKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQPSEDYQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     MHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKNPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVGRATFNQMIKELERSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            14913..15298
                     /gene="tnpA"
                     /locus_tag="VQU28_000785"
                     /pseudo
     CDS             14913..15298
                     /gene="tnpA"
                     /locus_tag="VQU28_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(15677..16306)
                     /locus_tag="VQU28_000786"
     CDS             complement(15677..16306)
                     /locus_tag="VQU28_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HET2476166.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(16316..17317)
                     /locus_tag="VQU28_000787"
     CDS             complement(16316..17317)
                     /locus_tag="VQU28_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HET2476167.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(17348..17989)
                     /locus_tag="VQU28_000788"
     CDS             complement(17348..17989)
                     /locus_tag="VQU28_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HET2476168.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(18008..18823)
                     /locus_tag="VQU28_000789"
     CDS             complement(18008..18823)
                     /locus_tag="VQU28_000789"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HET2476169.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            19094..19522
                     /locus_tag="VQU28_000790"
     CDS             19094..19522
                     /locus_tag="VQU28_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2476170.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPVKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEDFESGI"
     gene            19534..20724
                     /locus_tag="VQU28_000791"
     CDS             19534..20724
                     /locus_tag="VQU28_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HET2476171.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSAQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            20735..21226
                     /locus_tag="VQU28_000792"
     CDS             20735..21226
                     /locus_tag="VQU28_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HET2476172.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLACNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            21241..22020
                     /locus_tag="VQU28_000793"
     CDS             21241..22020
                     /locus_tag="VQU28_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2476173.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTANHVVEEEAHDDWI"
     gene            22007..22825
                     /locus_tag="VQU28_000794"
     CDS             22007..22825
                     /locus_tag="VQU28_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2476174.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVVIGIIGSWLKIIA"
     gene            22825..23118
                     /locus_tag="VQU28_000795"
     CDS             22825..23118
                     /locus_tag="VQU28_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2476175.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQTEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            23140..25041
                     /locus_tag="VQU28_000796"
     CDS             23140..25041
                     /locus_tag="VQU28_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HET2476176.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMEPCSKIHQIQPMVWDQVFEDDPEWAYMLNRQEYLLQFMIGYVVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDMLEEKELEKIL
                     VSLEKQIDFMKSYYRAKYTLSNWGILQTIPMLAIYYFFSDKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYRELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEILSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPKSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSNKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSEDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            25101..26102
                     /locus_tag="VQU28_000797"
     CDS             25101..26102
                     /locus_tag="VQU28_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2476177.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(26212..26547)
                     /locus_tag="VQU28_000798"
     CDS             complement(26212..26547)
                     /locus_tag="VQU28_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476178.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(26558..26800)
                     /locus_tag="VQU28_000799"
     CDS             complement(26558..26800)
                     /locus_tag="VQU28_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476179.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(26811..27353)
                     /locus_tag="VQU28_000800"
     CDS             complement(26811..27353)
                     /locus_tag="VQU28_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HET2476180.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(27369..27563)
                     /locus_tag="VQU28_000801"
     CDS             complement(27369..27563)
                     /locus_tag="VQU28_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476181.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            27729..28679
                     /gene="rsmH"
                     /locus_tag="VQU28_000802"
     CDS             27729..28679
                     /gene="rsmH"
                     /locus_tag="VQU28_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HET2476182.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNHYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            28691..29008
                     /gene="ftsL"
                     /locus_tag="VQU28_000803"
     CDS             28691..29008
                     /gene="ftsL"
                     /locus_tag="VQU28_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HET2476183.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            29012..31264
                     /gene="pbpX"
                     /locus_tag="VQU28_000804"
     CDS             29012..31264
                     /gene="pbpX"
                     /locus_tag="VQU28_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HET2476184.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDSFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAVYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKVEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            31266..32246
                     /locus_tag="VQU28_000805"
     CDS             31266..32246
                     /locus_tag="VQU28_000805"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HET2476185.1"
                     /translation="MFISISAGIVTFLLTLVGIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNMGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDMLSVFGYQVHLGIFYIVFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLSFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            32855..34960
                     /locus_tag="VQU28_000806"
     CDS             32855..34960
                     /locus_tag="VQU28_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2476186.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELERKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQEKVIGQDKAVEVVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVVIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            35683..36702
                     /locus_tag="VQU28_000807"
     CDS             35683..36702
                     /locus_tag="VQU28_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2476187.1"
                     /translation="MSNTLKQLLLFREEFIKNILNGIDVDETVVIENDFVEELENFLK
                     ENDLLDFSNSIFFQLSGDKVIVNNIYPGYLAYFNRFLTFYPKIFAIKEVGEYIDFLNF
                     EKRIADMFYCWGFNANSRVLTAEKIIEIPNHQINAKYIKKVIKMSELLFGVDSNNRIR
                     LLNNRRELIKTIFQGSVIKSAIPALAATLDTISNSGSFQISLSKQIINNLVLSGKDKV
                     TIPRIELDNVVLSRQKIIVSVSYINDFLDLRFPDKIEYILNFLEGSGFNTKVMVYFGK
                     IVEEDKNYFVNLEKPQYFDFTSILFNKLFFKELNKFDYMVIEELIPNLDEDVMMTEYI
                     KEYTE"
     gene            36715..>37031
                     /locus_tag="VQU28_000808"
     CDS             36715..>37031
                     /locus_tag="VQU28_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476188.1"
                     /translation="MILSKVRCYIYNSKIRGKVLYELYEFINENKQLMDAINSIKVDS
                     SNGFSIQILHLGEQQTTVLLTDFFTKTLDLFKEFRVTNKSTLEQVKNQISILNSSGPK
                     MDLT"
BASE COUNT        11788 a         6024 c         7932 g        11287 t
ORIGIN      
        1 atcgctataa gaaaaaatca ttttattggt ataaagaagt gattgaaagt aatggcgaaa
       61 cgttagataa ttagttcata aaaaacaagt ttagatcctc atctaaactt gtttttttaa
      121 aattgaagga tgattttgga ggatatgaga tgacattgac tattcatgta tattataaag
      181 gcgatggaga ttgtgcaata aagtttgcaa gagaaatgct tgaatctggg cttgtcgcag
      241 aaattagatg tcaaaaaggt aatttgaaat acgaatattt tttaccaatt gaaaaagagg
      301 gaattattct ccttattgat cagtggataa atcaagaagc tttagatgaa catcatcaat
      361 caaatacaat gcagaaaatc ttagatttaa gaaaaaaata tcacttgcag atgcaagttg
      421 aacgttatat agaagatgat tctgggatgc cagaaagcga taagtcgttt atagacacag
      481 ggaattagaa ttattgtgag agtttttgta gtttgaaagt attgaaaatc gtaaagatat
      541 cattacgaga atacaagaag atattgttct atttccatta aaaattactt ctttccttaa
      601 taataaggaa aaagaatacg ttttcatctt ttggtttgag gtttataata agaatatcaa
      661 agtcaggagg aaaaaagatg actactttta aagatggatt tttatggggt ggtgctgttg
      721 ctgctcatca acttgaaggc ggatggcaag aaggtggcaa aggaattagt gttgctgatg
      781 ttatgactgc tggtcgccat ggagtagctc gtgaaattac tttgggagtt ttagagggta
      841 aatattatcc aaatcatgag gcgatagatt tttatcatcg ttataaagaa gacatagcac
      901 tttttgctga aatgggattc aagtgcttcc gtacctctat tgcatggaca cgtatctttc
      961 caaaaggtga tgagttagag ccgaatgaag aaggattaca gttttatgat aatctttttg
     1021 atgaatgctt aaagaatggt attgaacctg tcatcactct atctcatttt gaaatgcctt
     1081 atcacttagt gaccgaatat ggtggttgga aaaataggaa attgattgat ttctttgctc
     1141 gttttgcaga agtcgtattt aaacgttaca aagataaggt taaatattgg atgactttca
     1201 atgaaatcaa taatcaagcg aattatcagg aagattttgc accatttact aactcaggta
     1261 ttgtatatga ggaaggtgat aatagagaag caattatgta tcaagcagca cattacgaat
     1321 tagttgcttc tgcacgagct gtaaaaattg gtcatgagat taatccagat tttcaaatag
     1381 gttgtatgat tgcgatgtgt ccaatttatc cagttacttg caatcctaag gatatcttaa
     1441 tggcaatgaa agctatgcag aagcgttatt attttgctga tgtgcatgtt ttaggtaaat
     1501 atcctgagca tattttcaag tattgggaac gaaaaggtat ttcagttgat tttactgacc
     1561 aggataaaga agatttactt ggtgggactg tagattacat tggtttcagt tactatatgt
     1621 cctttgctat agactctcat cgtgaaaata atccttattt tgattatctt gaaacagaag
     1681 atttagtgaa aaataattat gttaaggctt ctgaatggga gtggcaaatt gatccagaag
     1741 gtttacgtta tgcgttaaat tggtttacag accactatca cttaccactc tttattgttg
     1801 aaaatggttt tggagctata gatcaagttg cagcagatgg tatggtacat gatgattata
     1861 gaattgaata tctaggtgcc catattcgtg aaatgaaaaa ggctgtagtt gaagatggtg
     1921 ttgatttaat gggttatact ccatggggat gtattgattt ggtttcagct ggtaccggtg
     1981 aaatgcggaa acgttatggc tttatttatg tagataaaga tgacaatggg aagggaagtt
     2041 ataatcgttc cccgaaaaaa tcttttggct ggtataagaa agttatttta tctaacggtg
     2101 aatcagtaga atagtataaa caaactgaga ttattctcag tttgtttagc atattgagga
     2161 gacatatgat tggaagtatt gcagcaattt taacaacatt tgcattttta ccacaagttt
     2221 ttcgtgtagt taagacaaag gacacaggat ctattgctct aggtatgtat gttatgcaag
     2281 ttattggtat tgcattgtgg cttgctcatg gtattcgtat aggagacttg cctttgattt
     2341 tagcaaatag tgtttcattt ctactctcag gaataatttt attttataaa ttgaagtata
     2401 aataattttt ttccatatca tttaggaaaa tgagggaatt gtcattgaaa acgctttatt
     2461 ttataataaa aatagataac aaataagcca acttagcaca gctttgattg gatttaagaa
     2521 aaggagaaaa tattatggca aaagctttaa ttatttgtgc agcaggaatg tcttcatcac
     2581 ttatggcaca gaaaacaaca gagtttctaa aaggaaaagg gaaagatatt gtagttgatg
     2641 ctattagtgc tactgaagga gggaaagcta tcgcagcagc agcttttgat ttgtatcttg
     2701 tcagtcctca aacaaaaatg tactttaagc aatttgaaga ggctggttct aaagcaggaa
     2761 aaccagttgt acaaatacct cctcaagcct atattcctat tccaatggga attgaaaaaa
     2821 tggctaattt gattcttgag aatatttgat gaacacatga aatgtcaata aatatttaag
     2881 ttaagataaa ttgttttgag tttgatataa attgatttaa gtgtataata tgcttcaata
     2941 tatgaaaaca tacgttttag gagggaatga atgtgttaaa cattaaagag aaagatatcc
     3001 ttcaattttt aataaaaaat aaggaacgtt ttgttacaag caaagaactg gcagaatatt
     3061 tatcctgttc agatagaact gtccgtaatg tactgaaatt aattgagaag acaatgatta
     3121 ttcaaggagt ccggttgatt tcaaaacaag gtcaagggta tcaaatattt tttgagaatc
     3181 aaggcgctta ccaggagttt agacaaactt atgaactgga agaagactat acgaaaactg
     3241 cggtttcaaa aggtgatgat cgtttagtat ttatcttaaa taaattatta tttgagcaag
     3301 ttccagttct ttttgatgat ttagctgatg aactttacgt tagtcgttca acattatctc
     3361 atgatttcag aaagatacga gtgatgttat cagagtataa tttgtccatt gagagtaggg
     3421 cgaataaagg agtttatgtc tcaggcgagg aacgtgataa acgacgtttt atcataaatt
     3481 atttcctaga gaatcaattt tttaaaacaa tacattgcta tgtaaaattt aacttttttg
     3541 ctcaaactgt tccattagaa gaatttgcta gaattgtttt ggatgaatgc caagaggcca
     3601 acctaaaact atctgatttt gtactacaga atttagtagt tcacatagca ttgtctgtga
     3661 ttcgtttaaa atccggattt gaaataaaaa atatagactg tcagatgact gatgatgcaa
     3721 tagagagaaa ggttgctcaa agaattcttt ctaaagtaag agaagtaaca aatcaggaat
     3781 ttccagttca ggaaatagac tacattactt tgcatttgtt agctaagagt caacaatgtc
     3841 aaaaaaatca gaagaatatt tctgaagaag ttttaaaaaa atctttattt aaaacatttc
     3901 agaatttagg attagatgat atgtataact tttcttcaga tttccaacta attgaaggat
     3961 tgataactca tctaatgaca ctacaagtac gtttagaaag ccggattacc ttaaataacc
     4021 ctttggtaga tgaaattaaa cagaattata gtgatatatt ctttatgact agagaaattc
     4081 ttgcaaatat ggacaagttc ttggagtgga caatatcaga tgatgaagta gcatatgtaa
     4141 gtcttcactt tttagctgct atggaacgta gcaaagagag tactaagttt aatattcttg
     4201 ctatttgtgc aacaggattt ggtgcagccc aaatgttaag aaatcgtttg gaaacggagt
     4261 ttgggaagcg agtagaagtt gttgatgtaa taggctatta tgaattgaat caagaaaaac
     4321 ttaaaggaat agattttata gtgtcagctg ttgatttatc aaatctatat tttcaaattc
     4381 cagtttttaa agttagtgtg tttcttaaga gtgatgagat ggaaatgatt agaaaagcta
     4441 tggatcagat gcaagtttct agttatgtac aatcatcaaa gattaataaa tttgaaaaca
     4501 acagctttag acagtatttt agtaaagaaa attttttaat atgtactgaa tcagacaagg
     4561 taaatttact tgaaaagatg gttgagagtt tatctgttgg tgaatcaaat gagtttgaac
     4621 aatctttact ttatggaatt aaacagcgcg aagaattaag ttcagttgtt ttttctgaaa
     4681 aaattgcagt tccgcatcct attcagccct ttggtacaga gggaaaggtt tcggttgcaa
     4741 tctgtaaaga ttcgttactc tgggataatc aaagttcata tgtccagcta gtatttcttc
     4801 tatctccatc aatatatggg aacgaaggtt tggcaactgt gactaaaaag atagtctctc
     4861 tgacagagaa tgatgagttg caaaatcagc taatatcttg caataacttt gaagatttta
     4921 ttagcatatt tgagaagata aaataggagg ttcatatgaa tcaagaggaa ctgcaggttg
     4981 cagcatttga aattatcctg cattcgggaa atgcaaggtc agaaatacat gaagcatttg
     5041 caaaaatgag agaaggtagt tttgatgatg cagaaagtaa attaaatcaa tcaaatgaga
     5101 taattcttga agcgcatcat gcacaaacca aactcttgca agaatacgca agtggagtgg
     5161 aaattaaaat tgaaattatc atggtacatg ctcaagatca tttaatgacc actatgacat
     5221 tattagaagt agcaaaggaa atgttagctc tttataagaa agttaactaa atatgtgttg
     5281 ttatataagg aggaaatgat atggataaaa aagaacgaca aaagattgaa caacaacgtc
     5341 gagagatggc tctaactaat acttttttta atcgatatct tcttctacgt tattcaatag
     5401 cactcttctt ttttgggaat atttattggc ttttgaatca gtttattaat ccctcgccta
     5461 ttattatttt ccccattatg ctcatagtat tttctatttt agcaactgtt gaacaattta
     5521 aactctatgg aaataggaaa gaaaagttgg gaataacact aatgtttgtt agaatacaaa
     5581 tacttatttc aataggatta cttgttttaa cttggacaag ttggtttaag aatctttttc
     5641 cgatttttga aaataaccaa gtagcacgtt tatttgtttt tgtagttctt ctattaggtt
     5701 tagttctgag tttgttagac attagaagaa ttaaaaaaat ttataaacga acagataagg
     5761 cttatcaaca atttgtccaa ttggaaaaga actcacttag tttataatcc tgatctagta
     5821 taatttgtat ttgaaactag tctttagaaa gtaaaaatta ttatgtttta ttaaggaggt
     5881 ctatttatgg atcaacaaaa cgggttgttt ggttttcttg aaaaccatgt tatgggacca
     5941 atgggcaaac ttgctcagtt taaagtagtg cgtgctatca cggctgcagg tatggctgct
     6001 gtaccattta ctattgtagg atcaatgttt ttggtattca gtattttgcc acaagctttc
     6061 tcattttggc caattgtggc agatattttc tctgcttcat ttgataaatt cacatcactt
     6121 tacatggttg caaactatgc gactatgggt tctctatctc tttatttcgt tctatcactt
     6181 gcatatgaat tgacaaaaat ttatgcagag gaagaagaac tcaatatgaa tcctcttaat
     6241 ggtgccttgc tcgccttgat ggcttttgtc atgacagtac cgcaaatcat ttttgatggt
     6301 ggaatgatga agactgtgac aagtctaaaa gaaggtgcag taattgcaga tggatgggca
     6361 atgggaaatg gagtcgcacg ttttgggaca acagggattt ttaccgcaat cattatggca
     6421 attgtgactg ttcttattta tcgtatgtgt gttaaacata attgggttat taaaatgcct
     6481 gaagctgttc cagaaggagt ttctcgtgga tttaccgctt tggttccggg atttgttgtt
     6541 gcatttgttg ttatctttat caacggtctt cttgtagcaa tgggaacaga tatttttaaa
     6601 gtcattgcaa ttccatttgg ttttgtatcc aatctgacta attcgtggat tggtttaatg
     6661 attatttatc tattgactca actactttgg attgtaggta tccacggtgc gaacattgtt
     6721 tttgcatttg ttagtccaat tgctcttgct aacatggctg aaaatgctgc tggcgggcac
     6781 ttcgctgttg caggtgaatt ttctaatatg tttgtaattg caggtggttc tggtgcaact
     6841 ttaggactat gtttatatat tgcttttgcc tctaaatctg aacagcttaa agcaatagga
     6901 cgagcatctg tagttccagc cttatttaat attaatgaac cattaatttt tggattacct
     6961 attatctata atccagcctt ggctatacca tttattttag caccaatggt tactgctact
     7021 atttattacg tagcgaattc tctaaacttt attaagccaa ttatcgcaca ggttccatgg
     7081 ccaactccag tagggattgg agctttctta gggacagcag atcttcgagc tgtattagtt
     7141 gctctagtat gtgcatttgc agcattctta gtctatcttc cattcatccg tgtatatgat
     7201 caaaaattgg tgaaagaaga gcaaggtatc taataagtaa aaaagtatac tcgatgatag
     7261 aatccatata gtttaaataa tgagtgttaa tattagcagt gaagaggtct ttttaagata
     7321 ttcttctctg ctttttcata ttttttcgct atgtagggta tgaagcaaaa tagaaagtag
     7381 ttcgaatagt tgataaacag gaaactgctg ttatttaaaa aaaacaataa agtaaagtat
     7441 gttatcgtag aatacgatac aatagtaaaa aagcaaaaag aatgcgtttt atatttgggt
     7501 ttgaggttta taaaatagga taagcgtgct gaaaacttag atatgacaaa caaaataaaa
     7561 tttttaaaag ctacatagaa acatggtata atgtatctaa aggaggtagc atatgtcctt
     7621 agatatagat aaagaaaaga tgacaatcat gggaatagct tttgaaaaca gatccgtttt
     7681 taagagtgtt tggtatgctt taagtacaaa tatgattgaa ggatggcgac ctacggttag
     7741 tgatgttgaa aaattacgag atgaagctct tgctctgggg atgacttaat gaagcgcaac
     7801 tgttatgagt cttatgacta tgttgatccg gataatttat ataccgatcc gggttcttca
     7861 gttctaagaa ataagcagga aggaaaaaag cacgcgagct tgaatatcgt atgattgcta
     7921 gtaagagttt gaaattattt atcaatccta tcttagttaa tagtttaaaa gtagtttgtt
     7981 tcacaactgc ataagcttag aaaagttgaa aagtgataac tctttaataa actcttggtt
     8041 tgttaaaaga tattttgtcc actacatttt ttcaattgac tattatttgt catagatata
     8101 acagatagaa cagatagaac attgcattca gaataatgaa aaacgcatca aaaatggtgc
     8161 gttctgtttt tatgctacca taaaaggagg aaaacaaatt gaaaattttt aagggagagt
     8221 tttatcgaat ctctgtatta acagacaagc tagtaaggtt agaatactct caaactggaa
     8281 gttttgagga tagaacgaca caacttatct ataatagaga ttttggccaa gtttcgttag
     8341 attatatcga gacatcaaac gtactagata ttatgacgga ctattttcat ctgcacttta
     8401 ataaaggaga atttaacgcc gaaaatttat ttatagaatt aaaaggaaat tttgccgtat
     8461 atggtagtcg ctggtatttt ggtgaatcta ttgaaacgtt aaaaggaaca gctcggactc
     8521 tggataaggc agatggagca atctcgttag aagatggaat tattagccga aatggtatag
     8581 ccttattgga tgattctcaa ggatttattt gggatgaaca atctggttat attgagagag
     8641 aaaatcaaat tgacctgtat ttctttgcct atgggcatga ttatagagga gcaatcagag
     8701 acttttacca tttgactggt tcaacaccct tgttgccaag atatgcttta ggcaattggt
     8761 ggagtagata ttggccttat acgtcggatg aatacttgga tttaatagac agatttgaaa
     8821 cagagaaaat tccattatct atcggtgtgt tagatatgga ttggcatata actgacattc
     8881 cagctcgttt tggaagtggc tggacaggat atagttggaa tagaaactta ataccaaatc
     8941 cagaacagtt attgcaacaa cttcatgata gaaagttaaa actctcctta aatgtccatc
     9001 ctgctgatgg gatacgggct tatgaagaag cttatcctca aatcgcaaaa cggttggggt
     9061 taaatgtaga actagaagaa cctgctattt ttgatttttt taatccctct tttagggaag
     9121 cctactttaa agatgttcat tatgagctag aaaagcaggg agtagatttt tggtggattg
     9181 actggcaaca agggacacaa ggtatgctag atccactttg gcttttaaac cattatcact
     9241 atcaggatag ttgtaaaaat gcagaaggtg gtttgatttt atcaagatat gcaggtcctg
     9301 gtagtcaccg ctaccctgtt ggtttttcag gggatactat tattagttgg aattccttaa
     9361 ggtttcaacc ctattttaca gcgacagcat ctaatatcgg ttatagttgg tggagtcatg
     9421 atatcggtgg acatatgttg ggggattatg acgaagagct acaaactaga tggctacagt
     9481 ttggtgtttt tagtccgatt actcgattac atagttctag aagtcctttt aatagtaaag
     9541 aaccttggtt cttttcagaa acaacatcta agattatgaa gaaatacctt cgtttgagac
     9601 atcagatgat tccctatcta tataccatga atgtaaagac acatgaggaa ggtgccccat
     9661 taatcagtcc aatctattat ttctatccag agaatgatga gagctataat gttccaaacc
     9721 aatacttttt tggaacagaa ctgatggtgg ctcccattgt agaaaagatg gatttgacat
     9781 tccaatctgc aaaagtagat gtatggttcc ctgaaggtga atggtatgac ttcttttcag
     9841 agaaaaaata cacaggtggt gtgaagttaa gtgtttatag ggacatctcg actacgcctg
     9901 tgtttgcaaa aagtggtgca atcattccct tggttggttc tgagataggt atgggtgttg
     9961 atttacctga ggttgtagat tggtatgtat tcccagggaa acaacatttt tttgaaatgc
    10021 ttgaagatca aaatggtcaa agatataaaa caagattatc aatcgattgg gaaatgggaa
    10081 tggtagagtt agcattacaa ggagattcta gtatcgttcc aagcaataga aaacatagaa
    10141 ttcattttaa aggaacgaat gtgtctataa ttgaattgcc aaataagaat gatacagcta
    10201 aatttgaatg gaaagataat aaaaggacat ctttaaatga cgaagttttt agactactaa
    10261 agacagcttc tcttccatat gaattaaaag atagattgtt aaatcaattc atcaatgcga
    10321 aaaactctca tgacttaatg aatatcttgc atcatcagga taaggaattg agagggcgtt
    10381 tgttggaaat gatatttact agccaaaact aattgaaata attagaaagt catttgttta
    10441 gtgtatatga agattctgta tggtattcgt tatcactggt cacattgaaa atgtgaaata
    10501 agcacctaca acatagactt tacaacactt catgtttcat atcatggggt gtttttctat
    10561 ttaagtcaaa gcccatcaaa aaatctcact attcatcagg ttaaaggaaa cctaatcata
    10621 gtgagattgt gaattatagt agagtttgta tcgcctcttc aatttgtttt gggtctgttt
    10681 ttgaagaaaa gcgttcaaag acttgcccat ctcgaccgat gagaaactta gcgaaattcc
    10741 attcgattcg ttttcctagt gggccggatt tctggtcttt taaccagaca tagagagggt
    10801 ctgcttcctt accgttgacc ttaatcttgg caaaacgtgg gaaggtggtt tgaaaatgta
    10861 ggctacagaa ggcgttgatt tcctctgcgc tgccgggtgc ttgtcccata aactgattgc
    10921 aagggaaatc caatatttcg aagccctgat cttgatagcg ttcatagagt tcttgaagtc
    10981 cctggtactg gggcgttaaa ccacatccag tagcagtgtt gacaatcaag agaactttgc
    11041 cacgatagct atccaaggga gttgcttggt tgttttggtt caaaacggaa aaatcataaa
    11101 gtgaagtcat gagggcctct tttctttttc tacattgtaa cagtttttac ttttttcgtc
    11161 gagtcggatg gaagatattg ttgaagaata agccttcaat atgatagaaa ccgtttacaa
    11221 ataactttaa aaatggtatg atttaattaa accggtttac ttagttgcta taaatctcta
    11281 atttgagatt tagttcctat tgattttaca atatgtttat tggagtgtat acatgcaaac
    11341 aaaaacaaag aagctcattg tgagtttgtc ttcacttgtt ttatcaggat ttttattaaa
    11401 ccattatatg acagttggag cggaagaaac gactacgaat accattcagc aaagccagaa
    11461 ggaagttcag tatcagcaaa gggatacaaa aaatttagtt gaaaatggtg attttggtca
    11521 gacggaggac ggaagcagtc cgtggacagg aagcaaagct caggggtggt cagcttgggt
    11581 agaccagaag aatagtgcag atgcctcaac tcgagtcatt gaggctaagg atggggctat
    11641 cactatctca agccatgaga aattaagggc agcggttcac cgtatggttc ctattgaagc
    11701 taagaaaaag tataaactgc gtttcaagat taaaacagat cataaagtcg ggattgccaa
    11761 agttcgtatc attgaggaaa gtgataagga caagcgattg tggaattctg caacgacgtc
    11821 aggaacaaag gactggcaga ccattgaagc agactatagc ccgactttag atgttgataa
    11881 aatcaagctg gagttattct atgaaacagg aactgggact gtttccttta aggatattga
    11941 gctggtagag gtagcagacc agccttctga ggactatcaa acagataaac agcttgagga
    12001 aaagattgat ttaccaattg gaaaaaaaca tgttttttct cttgcggact atacttataa
    12061 ggtagaaaat cctgacgttg cttcagtcaa aaatggaatt ttagaacctc ttaaggaagg
    12121 gacaaccaat gtcattgtca gtaaagatgg caaggaagtg aaaaagattc ctttgaagat
    12181 tctagcctct gttaaggatg catacacaga ccgtttggat gactggaatg gcatcatcgc
    12241 tgggaatcaa tactatgatt ctaaaaatga acagatggcc aaattaaacc aggaattgga
    12301 aggaaaggta gctgatagcc tatctagtat ttcaagtcag gcggaccgca cctatttgtg
    12361 ggaaaaattt tcaaattata aaatgtctgc aaatctgact gccacttatc ggaaattgga
    12421 ggagatggcc aagcaagtga ccaatccttc ttctcgttat tatcaagatg aaactgtcgt
    12481 tcgaacagtc agggattcca tggaatggat gcataaacat gtctacaata gtgaaaagag
    12541 cattgttggg aactggtggg attatgaaat cggtacacct cgtgccatca acaatacctt
    12601 gtctctgatg aaagaatact tctctgatga ggaaattaaa aaatatacag atgtgattga
    12661 aaaatttgta ccagatcccg aacatttccg aaagacgact gataacccat ttaaggctct
    12721 aggtggaaac ttagttgata tgggaagagt aaaagtaata gctggtttac tgcgtaagga
    12781 tgatcaagaa atttcttcta ccattcgctc gattgagcaa gtgttcaagt tggtagacca
    12841 aggtgaaggt ttttatcaag atggatccta tatcgaccat accaatgttg cctatacggg
    12901 tgcttatggg aatgttttga ttgatggcct gtctcaactg ttgccagtca ttcaaaagac
    12961 caagaatcca atcgataaag ataaaatgca aaccatgtac cactggattg ataaatcgtt
    13021 tgctcctttg ctggtgaatg gagagctgat ggatatgagt cgtggacgct cgatcagtcg
    13081 tgcaaatagc gaggggcacg tggccgcagt agaagtacta agagggattc accgaatagc
    13141 ggatatgtct gaaggagaaa ccaaacaacg tttgcagagt cttgtgaaga ccattgttca
    13201 atcggatagt tattatgatg tctttaagaa tttgaagact tataaggata tcagtttgat
    13261 gcaatccttg ttaagtgatg caggagtcgc aagtgttcca agaacaagtt acctatctgc
    13321 ctttaacaag atggataaaa cagccatgta caatgcagag aaagggtttg gatttggctt
    13381 gtcactcttt tccagtcgta ccttgaatta cgaacacatg aacaaggaaa ataaacgtgg
    13441 ttggtatacg agtgatggga tgttctatct ttacaatggc gatttgagtc actatagcga
    13501 tggctactgg ccaacagtta atccatataa gatgcctggt acaacagaga cggatgctaa
    13561 gagagcggat agcgatacag gtaaagtttt accgtctgct ttcgttggaa cgagcaaact
    13621 agatgatgcc aatgcgacag caaccatgga tttcaccaac tggaatcaaa cattgactgc
    13681 tcataagagc tggtttatgc taaaggataa gatcgccttt ttaggaagca atatccaaaa
    13741 cacttcaaca gatactgctg caactacaat tgaccagaga aaactggaat caagtaatcc
    13801 atataaagtc tatgtcaatg ataaagaagc ctcccttaca gaacaagaaa aggattatcc
    13861 tgaaacccaa agtgtctttt tagaatcatc cgattcgaaa aagaatattg gttacttttt
    13921 ctttaagaag agttcaatca gtatgagtaa ggctttgcaa aagggagcct ggaaggatat
    13981 caatgaagga cagtcagaca aggaagttga aaatgaattt cttacgatta gtcaggctca
    14041 taagcaaaat ggagattctt atggctatat gctcatccct aacgtgggtc gtgccacctt
    14101 caatcaaatg ataaaagagt tagaaagaag cctcatcgaa aataacgaaa cccttcagtc
    14161 tgtttatgat gccaaacaag gagtttgggg cattgtgaaa tatgatgatt ctgtctctac
    14221 tatttccaac caattccaag ttttgaaacg tggagtctat accattcgaa aagaagggga
    14281 tgaatataag attgcctact ataatcctga aacccaggaa tcagctccag atcaggaagt
    14341 ctttaaaaag ctagagcaag cagctcagcc acaagtacag aattcaaaag aaaaggaaaa
    14401 atctgaagag gaaaagaacc attcggatca aaagaatctc cctcagacag gagaaggtca
    14461 gtcaatcttg gcaagtctag ggttcttgct acttggggcg ttttatttat tccgtagagg
    14521 aaagaacaac taatttgttc atcatctaga tgaattacat gaaactgtgg aaaatagaaa
    14581 aaatccgaag aaggccaaat atcttcggat ttttctttta aaaaaggata agttgatact
    14641 tatccttttt acttcattat gtgactatag cgttttgaaa ctagaatagg acatcataac
    14701 tgctaaaaga tttctataaa ttcatttgat tttcctaatc aatttattcg tatcctattt
    14761 cactctacta tattagtccg tctcgcacct cacggagcga gacaaaacaa ccacccgcta
    14821 tgcgggtgcg cgtcgaaggt tataccaaaa aaactccaaa cgcgatacaa taaaggtgtt
    14881 caatccactt gtaaagcgaa aggagaaaaa atatggcaca aaaggctcat agtttatctc
    14941 acacaaagtg gaggtgttct atcacattgt gttcacccct aagtatagac gaaaagtcat
    15001 ctataatcaa tatagaagta gtttaggcga aatatttcat cgcttgtgta gttataaagg
    15061 tgttgaaatg atcgagggtc acttaatgcc agaccatgta cacatgttag tcagtattcc
    15121 actaaggcta agtgtttcga gtttcatggg ttatttaaaa ggaaaaagtg cactcatgat
    15181 gtttgacaaa cacgccaatc tcaagtacaa gtttgggaat cggcatttct gggtggaagg
    15241 ttattatgta agtccagtag ggcttaatga agccacaatt aagaaatata gtcaagatta
    15301 aagaaattat ccagtggatg atttcttcac gagtatgaaa atttgagaac tagtaaagta
    15361 tgatatatag taaaatgaaa taagaacagg acaaaatcga tcaggacagt caaatcgatt
    15421 tttaacaatg ttttagaagt agaggtatac tattccagtt tcaatctact atagcactag
    15481 ataaattgag tgtaaaagaa tatgaggatc cctttaggga tagtggtaag taataccaaa
    15541 gtctcttaaa gaggcaagtg acgagtcaag agcaataagg cttgaacaac gtaaaagcca
    15601 gcgtctttag gcgctggctg atgatttggg cttatagctc tgggataaac cacccgttag
    15661 acgggtagtt atgattttat ctgagtgtaa tatactgttg ggcaatctcg ctgatgcggt
    15721 caaagttgcc ttgggaagcg agtttattga gttcgccacc aattccaacg gcatctgcac
    15781 cagcagcgaa ccattgaggg atgttgttta ggccgactcc tccggttacc attacggaaa
    15841 cttgtgggat cggtgccttg actgcagaga tatatgctgg actgagagta ctacctggga
    15901 agagtttgat gatttcacta ccggcttcaa gtgcagtcgt gatctctgtg agggtaatac
    15961 agcctggaat gtacggtgtg ctgtagagat tgcacatttt cgcagtttca gcatggaaag
    16021 atggagaaac aacgtaattt gctccagcta gaatggcatc tctagcagtt acggcatcaa
    16081 gcacagtacc tgcaccgata caaacactct gatcgtcctg atacaagtct acaagttcct
    16141 tgatgatttg tcctgcatac tgattggtat aggcgatttc aatagctttg ataccgccct
    16201 tgatacaagc aatcgaggct tgtagtcctt cttcctttgt atttcctcga atgacagcga
    16261 caattttctg tttttttagt tcaataatcg tatctgattt ggtcatgtaa ttctcctaac
    16321 gaatgatatc ttgtgcattt gccagtaaat tttcaatact agttgcggaa gtggagagat
    16381 ggtctcctgg aagagtgcat ttgagagttg cgctcgcaac tgcaaagtca atggtagttt
    16441 ttagggagga atgatggagt agttggtaaa gggcaccaga tataaaggca tccccgctac
    16501 caattcgttg atagactgca gtttttagtt ggacagactc ttcaaatcgt tcttccagag
    16561 cataggcttg atagacattt ttgtcttgct catcactaga tcggagggta tggaaaatgg
    16621 ccttgaaacc ataggcttct tttaaaagtc gcatgcgatt ttccacctct tctaggctag
    16681 cactgtctct tggaaacatc tctagatttt ggtcatcaat catgagagga tcaataccga
    16741 agcaatagtc agtaaaacgt gcaaacttag aaaattcata cttggcttct aggactgaaa
    16801 tcatctttgt tctcagattg agatccattg aaacgacaat tcctcggcgc ttggcttctt
    16861 ccaagagtag gagaaggatc gcacggacct cttgaccgat agctatggtg attccactaa
    16921 aatgaaaatg gctaatcccc tgaaagagag aatccatatc tagcatgttt ggccgaatct
    16981 ggctgatact cgtatgctta cgatcgtaga aaacttcact ttgacgacaa ccaaagccgt
    17041 tctccaaata gtagaggccg attcgatcgc caagccgaca gattgaactg gtatcgattt
    17101 ggtgctgttt caagaatgtg agaaaacgat ctccaatctc gttggcaggg agtgcggtaa
    17161 agactttcgt tgagataccc agggcttgca aattacaagc gatgttaatt tctgatccgc
    17221 caaaataagt cgaactggca acatgatcgc cgatactggt ggcatctaat ggtgagattc
    17281 gaattaatgg ttcgccaaac agtaatattt tcgtcatcca ttagttcctt tctggtttag
    17341 aatagcttta cttggctaat acttctttca aataagcagc gacttcatca tcttggcagt
    17401 tttcaaagaa gtattcttgg aagtatttgc cagagatggt gtctttgagg aagtcttggt
    17461 cgattgtttt aaggatggtc atcaaatcat tgtgggtgat ttgtttcacc tcgtttaaga
    17521 tacgagcgtt gcgttgttca gggattacac gttctcttgg gtagccaccg cccatttctt
    17581 cagcaaataa gcgttcaaac atcaatttga gggtcagttc tgccccccag ccaaatcctt
    17641 tggcataagg gatagacaag gcgttaccac cattgatttg agaataaagg taagcgtcag
    17701 ttgggtccac tgctagacca cagacaacac cagggaagct gtttaaagca agcatagccc
    17761 ctacacccgt accacagccg gtaacaacaa agtcaactgc ctttgtattt aaaaggatgg
    17821 cagccattag tccgttctgc acataagtta attgactttc tccttcttct ccacgcatac
    17881 catagttaaa taattggtag tcttttttat ctgtcgcttc ttttagacta tcgtaaataa
    17941 tgtgattctt gctagcttga ctattttcat tgattaatgc gattttcata agatcttctt
    18001 tctattttta ctcaggttgt tttccgatgt aggctaagat accgccatct acatatagga
    18061 tgtggccatt gacaaaattg ctggcatcac tagcgagaaa gacagcaggg cccatcaaat
    18121 cttcaggatt tccccaacgt gcagcaggtg tttttgcaat gatgaactgg tcaaatgggt
    18181 ggcgagaacc atcttcttgc aactcacgaa gaggtgctgt ttgaggagtg gcaatataac
    18241 ccggtccaat tccgttacat tggatattgg ctccaccgta ttcagacgca atgttgcggg
    18301 tcaacatttt caagccccct ttagcagcag cataagcgct aactgtttca cgtcccagtt
    18361 cgctcatcat cgaacaaata ttgataatct ttccatgccc tttctttatc atagaaggaa
    18421 taactgcctt tgaaacgata aatggtgcgt ttaagtcaat atcgatgacc ttacggaaat
    18481 cagcggcgct catttcgcac attggaactc ggcggataat accagcgtta ttaacgagga
    18541 tgtcaatgac accaacctct tgttcgattt gcttgaccat ggcttggata ccgtcctcgt
    18601 ctgtcacgtc acagacatat ccatgggctt cgatgccaac ttcacgataa gcttcaatcc
    18661 ctttattgac cagatcttga ttgatatcgt taaagacaat agtagcgccg gcttcagcgt
    18721 aggatttggc aatagcaaat ccaattccat aagaagcgcc ggtgatgaga gcaatttttc
    18781 cttttaaaga aaactgctca attgagaatg atgtatttgt catttgatac ttcctttcaa
    18841 atttgttaag acttctctat aaccaaatta tattctagca aaccggtttt gtcaataaat
    18901 aaatattttt atttataaaa agatttgttt tcctgaaaaa cggaatgttt tgaaatttct
    18961 gtttattaaa atggttaaaa agataatgaa agcgctaata tttttttaaa aaaactattg
    19021 acaagataaa aataaaggat tacaatgggg aatataaagt aaaccggtaa acctaaacaa
    19081 gaaaggagaa aagatgaaaa ttgtacttgt agggcatgga cattttgcta cagggattta
    19141 tagttcttta caattgattg caggtaatca agaaaatgtg gaggcgattg actttgtgga
    19201 aggaatgtca gcagatgaac tcaagcaaaa aatcttactt gcaatttcaa atgaagaaga
    19261 agttttaatc ctaagtgatc tcttgggagg atcgccattc aaggtttctt ctaccataat
    19321 gggagaaaat ccagtcaaaa caatgaatgt tctctcgggt ttgaacttag ccatgttaat
    19381 ggaagcagtc tttgctagaa tggctcatag ctttgatgag gttgttaata aatcagtagt
    19441 ggcggcccag ggcggagtcg taaatggtaa agaattgttt tcaacggatg cagaggaaga
    19501 agatttcgaa tcgggtattt aaagggtaaa agaatgataa aaaaggttac gattgaaaaa
    19561 ataaaatcgc ctgagcgctt cttagaagta ccacttctga cgaaagaaga agtcggccag
    19621 gcaatcgata aggttattcg gcagttagaa ctcaaccttg actatttcaa ggaagatttc
    19681 ccgacgccag ctacctttga taatgtctat ccaatcatgg ataacacgga atggaccaat
    19741 ggtttctgga caggagaact gtggttggct tatgaataca gtcaacagga tgcatttaaa
    19801 aacatcgctc ataaaaatgt tctttctttc ctggatcgtg tcaataagag agtagaattg
    19861 gatcaccatg atctcggctt cttgtacaca ccgtcttgta tggctgaata taagataaat
    19921 ggagatggag aggctagaga agcaaccttg aaagctgcag ataagttgat tgaacgctat
    19981 caagaaaaag gtggttttat tcaagcttgg ggagacttgg gcaagaaaga gcattaccgt
    20041 ttgattatcg actgcttgct caatatccaa ctcttattct ttgcttatca agaaacaggc
    20101 gatcaaaaat actacgatat tgcagaaagt catttctatg cttcagctaa taatgtaatc
    20161 cgtgatgacg cttcgtcctt ccacaccttc tattttgatc ctgagacagg tcaacccttt
    20221 aaaggtgtaa cgagacaagg gtatagtgat gattcatgct gggcacgtgg tcaatcatgg
    20281 ggagtctatg gtattccttt gacttatcgt cacttaaaag acgagtcctg ctttgacttg
    20341 tttaagggtg tgaccaatta tttcttgaat cgtctgccaa aagatcatgt gtcctattgg
    20401 gatttgattt ttaatgatgg tagtgctcaa tcacgagatt cttcagcaac agctatcgcc
    20461 gtctgtggga ttcatgaaat gctaaaacat ctcccagagg tggatgctga caaagatatt
    20521 tataaacatg ctatgcatgc catgcttcgt tccttgatcg aacattatgc aaatgatcaa
    20581 tttacccctg gtgggacaag tcttctccac ggtgtgtact catggcattc aggtaaagga
    20641 gtggatgaag gcaatatctg gggtgactac tattacctag aagcccttat ccgtttctac
    20701 aaagactgga acctatattg gtaggaggag aaatatgaca atgccaaata ttattatgac
    20761 ccgtatcgat gaacggttga ttcatggaca aggacaactt tgggtaaaat acctagcttg
    20821 taatacggtc attgttgcca atgacgaagt aagcacggac aagatgcaac aaactctgat
    20881 gaaaacagtt gtgccagact cagttgccat gcgtttcttc cctttgcaaa aggtgattga
    20941 tatcattcac aaggctaatc ctgctcaaac gatctttatc gttgtaaagg atgtaaagga
    21001 cgctttaacc ttggtagaag gtggtgtccc tatcaaagaa atcaatattg ggaacattca
    21061 caatgcccct ggtaaagagc aagtgacacg ctccatcttc ctgggtgaag aggacaaggc
    21121 agccctcaag gaattaagcc aaactcatca agtaacattt aatacgaaaa caactccaac
    21181 aggaaatgat ggagctgttc aagtcaacat tatggactat atttaaaaga ggagatcgtt
    21241 atgtcgatta atgtatttca agcgatttta attggattat ggacagcttt ctgttttagt
    21301 ggaatgctgt taggaattta caccaataga tgtattgttc tgtcatttgg tgtcggaatt
    21361 attctaggtg atctgcctac tgctcttgca atgggagcta ttggtgaatt ggcttatatg
    21421 ggattcggtg ttggtgctgg aggtactgtt ccaccaaacc caatcggacc tggtatcttt
    21481 ggtaccttga tggctatcac tagtgctggt aaagtcagtc cagaagcggc tcttgccctc
    21541 tctactccga ttgctgtggc gattcaattc ttacaaactt tcgcctacac tgtacgtgct
    21601 ggtgcgcctg aaacggcgat gaagcacttg aaaaaccata atttgaagaa attcaagttt
    21661 actctaaatg caacaatttg gttgtttgcc tttattggat ttaccttggg ttgcttgggt
    21721 gccctttcaa tggatacctt gttgaaactc gtagactaca ttccaccggt attacttaca
    21781 ggtttgacag ttgctggtaa aatgctccca gctatcggtt ttgcgatgat cttgtcagtg
    21841 atggctaaga aagagttgat tccctttgtc ttgttgggat atgtttgtgc agcttatcta
    21901 aacatcccaa caattggtat tgcaattgta ggtactatct ttgctttgat tgaattttat
    21961 aacaagccaa aaacagcgaa tcatgtggta gaggaggaag cacacgatga ctggatctaa
    22021 caaattaaca aaacgtgatt atcttaaaac gtctttgcgg gcattctttt gtcaaaatgg
    22081 atttaactat agtaactatc aagggttggg atatgccaat gtgatgtatc ctgctttgaa
    22141 aaaacactat ggagaggatc aggaaggttt ctaccaagcc ttggaagaaa actgtgaatt
    22201 ctataatacc aacccacact tcctgccttt tattaccagc ttgcatcttg taatgttgga
    22261 aaatggccgt ccggcaaaag aaacacgtag catcaagatg gccttgatgg gaccattggc
    22321 aggtattggg gattctcttt ctcaattctg tttagctcca ttgttctcaa ccatcgcagc
    22381 ttcgtttgct caagaaggct tggttgtcgg tccaatcttg ttcttccttg cgatgaatac
    22441 gattttaaca gcgattaaat tgtcaactgg tctgtatgga tacaaactag gaacaactgt
    22501 gattgataaa ctaagcgaac agatggcaac gatttctcgt attgccaata ttatcggtgt
    22561 aaccgtaatt gctggtttgg cagcgacatc tgttaagatt atggtgccga ttacctttgc
    22621 tgcaggggaa gttaaagcag acgctaaaca aagtatcgta agtattcagg gaatgcttga
    22681 taaggttgct ccagctcttc taccagccct atttacactt ttagtttact acttgatcaa
    22741 agaaaagaaa tggacaacat ataaactcgt tattttaaca gttgtcatcg gaattatcgg
    22801 aagctggctt aagattatag cttaatggat acaacattgt tttatggaat agtgattgtc
    22861 ttggcagtga gtcccctttt actgtcaagc tttcattcta ttcgtcagca aaagttgctt
    22921 cgcaaacaga cggagcaacg acaagagtat ttagcttctt taacatctgg tgatgaagtg
    22981 ttgttattgt ctggaattca tggaaaaatc atttctatca aagatgactt ggtctccttg
    23041 cagattgcaa aaggtgtggt catctatgta gaaaaggaaa gtgtaatggg aaagacaaaa
    23101 gaactgcttt ttaagtagtt ctttttctgg taatagataa tgaaagaaga aagaagacaa
    23161 ttttttgaaa gagtcgatgg aaaccaatgt cgtgattata tcttgtcgca ctgttcaaaa
    23221 gactatgaga aagtcaagag ttcccttgaa cgcttgatgg acaatcgttt tatgtttgat
    23281 agtccttggg atatggagcc ttgttcaaaa atccatcaaa tccagccgat ggtatgggat
    23341 caagtatttg aagatgatcc agaatgggct tatatgctca atcgacaaga atatctcttg
    23401 cagtttatga tagggtatgt ggtagaagga gataaggact atattcaaaa gtgcaagttc
    23461 tttctatttg attggattga gcaggtgaga gaattttctc ctcaatcctt gatgactaga
    23521 accttggata cgggtattcg ttcctttact tggttgaaac tactcttgct cctcttgaaa
    23581 tttgacatgc tagaggagaa agaactagag aaaattttgg tcagtctaga aaagcagatt
    23641 gactttatga aaagctacta tcgcgccaag tacaccctta gtaactgggg gattttacaa
    23701 acaattccga tgcttgctat ctattatttc ttttcagata agatggacct agaagaagct
    23761 taccattttg cttcagagga gttgaaacag caaattgaga cacagatttt aggagatgga
    23821 agccagtttg aacagtcgat tctctatcat gtagaggttt ataaagcctt gctggatttg
    23881 tgtctcttgc ttccagactt gcaagatagt taccgagagt tgctggaaaa gatggcgacc
    23941 tatattcaaa tgatgacagg cttagatgga cgaactttgg cttttggtga tagcgattct
    24001 acagaaacga cagaaatttt gagcctgtct gctgtggttt tgaacaagga agaccttctt
    24061 aacggtctgg atgttaaagt cgatttgctt agcctcttgt tcctggggcg agaaaaggtc
    24121 aagcgactgc aggaatttga aaagagagct tggcagccta agtccatgat ctttgaagac
    24181 tctggacatg tctgcattaa ggatgaacat cgttatctat ttttcaaaaa tggtccgcta
    24241 ggaagtgccc atagccatag tgacgagaat agtttttgct tacagtatca aggacaacct
    24301 attttcatag atgctgggcg ttattcttat cgggagatat atgaacgtta tctcttaaag
    24361 agtgcttgga gtcattcgac ctgcattgta gatgggaaag ctccggaaag aatcacggga
    24421 tcctgggaat atgaatacta tcctcactcc ctgttttgtc accataaaga aagggaggga
    24481 gtgcattata ttgaaggggc ttattggtca gcagaacctg atttgcctta tcttcacaag
    24541 agaaaaatcc tcatgttggt agaggatgtc tggctcttgg tggatgacat caggtgtcaa
    24601 ggtcagcatg aggcgttgac tcagtttatc cttgacaagg atgtgaccta tcaagatggg
    24661 aaaatcaatc agttgagact atggagtgaa gttgattttg atttggaaga taccatcatt
    24721 tctcctaaat acaatgagct tgaaagaagt aacaaactca ccaagcgcca attctttgag
    24781 aatcagatgc tggattatac catcattgcg catgagagtt ttgaaatcat ccgtcattct
    24841 gtctaccaga cagatgatcg tgaagtggaa aatgctctgg cttttgaagt gaaaaatgac
    24901 gaaacagaca agctgattct gttattaagc gaggatattt gtgtaggtga aaaattgtgc
    24961 ctcgttgacg gaacaaaaat gcgtggaaaa tgtctagtat atgataaaat aaatgagaga
    25021 atgattcgct tgcagtgcta gaaataggca ttttgaatag tgaatatgtt ataataagta
    25081 ttagtaggag gtgttttaga ttggagaaga aactgaccat aaaagacatt gcggaaatgg
    25141 ctcagacctc gaaaacaacc gtgtcatttt acctaaacgg gaaatatgaa aaaatgtccc
    25201 aagagacacg tgaaaagatt gaaaaagtta ttcatgaaac aaattacaaa ccgagcattg
    25261 ttgcgcgtag cttaaactcc aaacgaacaa aattaatcgg tgttttgatc ggtgatatta
    25321 ccaacagttt ctcaaaccaa attgttaagg gaattgagga tatcgccagc cagaatggct
    25381 accaggtaat gataggaaat agtaattaca gccaagagag tgaggaccgg tatattgaaa
    25441 gcatgcttct cttgggagta gacggcttta ttattcagcc gacctctaat ttccgaaaat
    25501 attctcgtat catcgatgag aaaaagaaga aaatggtctt ttttgatagt cagctctatg
    25561 aacaccggac tagctgggtt aaaaccaata actatgatgc cgtttatgac atgacccagt
    25621 cctgtatcga aaaaggttat gagcattttc tcttgattac agcggatacg agtcgtttga
    25681 gtactcggat tgagcgggca agtggttttg tggatgcttt aacagatgct aatatgcgtc
    25741 acgccagtct aaccattgaa gataagcata cgaatttgga acaaattaag gaatttttac
    25801 aaaaagaaat cgatcccgat gaaaaaactc tggtatttat ccctaactgt tgggccctac
    25861 ctctagtctt taccgttatc aaagagttga attataactt gccacaagtt gggttgattg
    25921 gttttgacaa tacggagtgg acttgctttt cttctccaag tgtttcgacg ctggttcagc
    25981 cctcctttga ggaaggacaa caggctacaa agattttgat tgatcagatt gaaggccgta
    26041 atcaagaaga aaggcaacaa gtcttggatt gtagtgtgaa ttggaaagag tcgactttct
    26101 aaaatgaagg aaaatgactt gcaatctctg ttaagaaata aaataatccc acctagaaca
    26161 agctaggtgg gattatttgc ttatgaaatg agaaattatg ggagcaagct cctaaatcaa
    26221 ctgtttttga tctacttctt taactacttg ataaaagtta tagaagtagg ccaaacttga
    26281 aatgatggtt acgactagga atattgaaaa tttccattgg acagggttgg ttaaaagttg
    26341 tggaaaggat atgaggagaa agaagagggc tgcgttgagg acaggtatcc gttttgattg
    26401 tattttctca agtcctttat tgagcgcagg aagaaagagg agtaggagta gtaaaactgt
    26461 atgagaaaca gctcctgaaa taagggcgaa gaaaaggaaa atactgataa aaacatgaat
    26521 gttcagtagt ctagctagtg atttcataag gcacctccta atcctggtct tttttagctc
    26581 ttgcaatacg aagtgagtcg acaatatgta tcatcactcc gaaaaagaaa gctcccagta
    26641 tagttttaaa aatatgtttt gtatttagaa gagaactgat aaaatttgga ttttcacttg
    26701 ttagggtatc aatgagtgga attataaaaa atatcactgt tccataaatc gaacctgctt
    26761 tcagaccagg ataacgtaac tgtttctttt cttttttcat gagtttcctc ctaatcctca
    26821 tcttgatttt tcttagtttt tgcaatgcga cgggagatga ggaactgtat gctcgctccg
    26881 aagaaaatag aaccgagaat acttgataca ccatttctta tagtgagaag agaatgaaaa
    26941 tagtcctgac cttcatctat gagtatactg agaagaggag ttataaaaaa catccataga
    27001 ccaaagaaca aacctgcttt cagacctggg tagtgtagtt gcttgctttc tttctcattc
    27061 agcatatctg gttcaatgac tgtgatgcct gtttttttca tttggtaggt gacatagcca
    27121 gcagcgatga gggcaatcac taaaatcaga ggaggataga ttagagccac ttcttgaggg
    27181 tatttatagg ctagaaggag tggaataaga tttccgaaaa tcatcagata aaagaggacg
    27241 ataaagactt ggttcccaat actatcggcc tcacgccgtt tgtattcgtc aaggggacca
    27301 gaaataccgt atgtgcgttt gatcagtttt tcagtgaagg tttctttttt catgagtttg
    27361 ctcctttttt aaaaatcttc ctcccaaaag agactgttga ggtcagtttg gaggctgcgg
    27421 gcgagattga gacagagttc caaggttgga ttgtacttgt cgttttcaat catattgata
    27481 gtctgtctcg agacaccgat atccttggcg agttcgagct gggaaatacc caattccttg
    27541 cgaaattctt tcacacgatt catctggtct cctttctgat ttatgtcgta tatatttgac
    27601 tatattatag tcttttaaac ataaagtgtc aagtatattt gacatatttt ttgaagaaat
    27661 agtagtctcc ttgtcctatt tgtctgacaa gtgcaagctg gtcggatttg tggtaaaata
    27721 gataagatat gacaaaagaa tttcatcatg taacggtctt actccacgaa acgattgata
    27781 tgcttgacgt aaaacctgac ggtatctacg ttgatgcgac tttgggtgga gcaggccata
    27841 gcgagtattt attaagtaaa ttaagtgaaa aaggccatct ctatgccttt gaccaggatc
    27901 agaatgccat tgacaatgcg caaaaacgct tggcacctta cattgagaag ggaatggtga
    27961 cctttatcaa ggataacttc cgtcatttac aggcacgttt gcgcgaagct ggtgttcagg
    28021 aaattgatgg aatttgttat gacttgggag tgtctagtcc tcaattggac cagcgtgagc
    28081 gtggtttttc ttataaaaag gatgcgccac tggacatgcg gatgaatcag gatgctagtc
    28141 tgacagccta tgaagtggtt aatcactatg actatcatga tttggttcgt attttcttca
    28201 aatacggtga ggataaattc tctaaacaga ttgcgcgtaa gattgagcaa gcgcgtgaag
    28261 tgaagccgat tgagacaacg actgagttag cagagattat caagttggtc aaacctgcca
    28321 aggaactcaa gaagaagggt catcctgcta agcagatttt ccaggctatt cgaattgaag
    28381 tcaatgatga actgggagcg gcagatgagt ccatccagca ggctatggat atgttggctc
    28441 tggatggtag aatttcagtg attacctttc attccttaga agaccgcttg accaagcaat
    28501 tgttcaagga agcttcaaca gttgaagttc caaaaggctt gcctttcatc ccagatgatc
    28561 tcaagcccaa gatggaattg gtgtcccgta agccaatctt gccaagtgcg gaagagttag
    28621 aagccaataa ccgctcgcac tcagccaagt tgcgcgtggc cagaaaaatt cacaagtaag
    28681 agggaaaaag atggcagaaa aaatggaaaa aacaggtcaa atactacaga tgcaacttaa
    28741 acggttttcg cgtgtggaaa aagcttttta cttttccatt gctgtaacca ctcttattgt
    28801 agccattagt attattttta tgcagaccaa gctcttgcaa gtgcagaatg atttgacaaa
    28861 aatcaatgcg cagatagagg aaaagaagac cgaattggac gatgccaagc aagaggtcaa
    28921 tgaactatta cgtgcagaac gtttgaaaga aattgccaat tcacacgatt tgcaattaaa
    28981 caatgaaaat attagaatag cggagtaaga tatgaagtgg acaaaaagag taatccgtta
    29041 tgcgaccaaa aatcggaaat cgccggctga aaacagacgc agagttggaa aaagtctgag
    29101 tttattatct gtctttgttt ttgccatttt tttagtcaat tttgcggtca ttattgggac
    29161 aggcactcgc tttggaacag atttagcgaa ggaagctaag aaggttcatc aaaccacccg
    29221 tacagttcct gccaaacgtg ggactattta tgaccgaaat ggagtcccga ttgctgagga
    29281 tgcaacctcc tataatgtct atgcggtcat tgatgagaac tataagtcag caacgggtaa
    29341 gattctttac gtagaaaaaa cacaatttaa caaggttgca gaggtctttc ataagtatct
    29401 ggacatggaa gaatcctatg taagagagca actctcgcaa cctaatctca agcaagtttc
    29461 ctttggagca aagggaaatg ggattaccta tgccaatatg atgtctatca aaaaagaatt
    29521 ggaagctgca gaggtcaagg ggattgattt tacaaccagt cccaatcgta gttacccaaa
    29581 cggacaattt gcttctagtt ttatcggcct agctcagctc catgaaaatg aagatggaag
    29641 caagagcttg ctgggaacct ctggaatgga gagttccttg aacagtattc ttgcagggac
    29701 agacggcatt attacctatg aaaaggatcg tctgggtaat attgtacccg gaacagaaca
    29761 agtttcccaa cgaacgatgg acggtaagga tgtttataca accatttcca gccccctcca
    29821 gtcctttatg gaaacccaga tggattcttt tcaagagaag gtaaaaggaa agtacatgac
    29881 agcgactttg gtcagtgcta aaacagggga aattctggca acaacgcaac gaccgacctt
    29941 tgatgcagat acaaaagaag gcattacaga ggactttgtt tggcgtgata tcctttacca
    30001 aagtaactat gagccaggtt ccactatgaa agtgatgatg ttggctgctg ctattgataa
    30061 taataccttt ccaggaggag aagtctttaa tagtagtgag ttaaaaattg cagatgccac
    30121 gattcgagat tgggacgtta atgaaggatt gactggtggc agaatgatga ctttttctca
    30181 aggttttgca cactcaagta acgttgggat gaccctcctt gagcaaaaga tgggagatgc
    30241 tacctggctt gattatctta atcgttttaa gtttggagtt ccgacccgtt tcggtttgac
    30301 ggatgagtat gctggtcagc ttcctgcgga taatattgtc aacattgcgc aaagctcatt
    30361 tggacaaggg atttcagtga cccagacgca aatgattcgt gcctttacag ctattgctaa
    30421 tgacggtgtc atgctggagc ctaaatttat tagtgccgtt tatgatccaa atgatcaaac
    30481 tgctcggaaa tctcaaaaag aaattgtggg aaatcctgtt tctaaagatg cagctagtct
    30541 aactcggact aacatggttt tggtagggac ggatccggtt tatggaacca tgtataacca
    30601 cagcacaggc aagccaactg taactgttcc tgggcaaaat gtagccctca agtctggtac
    30661 ggctcagatt gctgacgaga aaaatggtgg ttatctagtc gggttaaccg actatatttt
    30721 ctcggctgta tcgatgagtc cggctgaaaa tcctgatttt atcttgtatg tgacggtcca
    30781 acaacctgaa cattattcag gtattcagtt gggagaattt gccaatccta tcttggagcg
    30841 ggcttcagct atgaaagact ctctcaatct tcaaacaaca gctaaagctt tggagcaagt
    30901 aagtcaacaa agtccttatc ctatgcctag tgtcaaggat atttcacctg gtgatttagc
    30961 agaagaattg cgtcgcaatc ttgtacaacc catcgttgtg ggaacaggaa cgaagattaa
    31021 aaacagttct gctgaagaag ggaagaatct tgccccgaat cagcaagtcc ttatcttatc
    31081 tgataaagta gaggaagttc cagatatgta tggttggaca aaggagactg ctgagaccct
    31141 tgctaagtgg ctcaatatag aacttgaatt tcaaggttcg ggctctactg tgcagaagca
    31201 agatgttcgt gctaacacag ctatcaagga cattaaaaaa attacattaa ctttaggaga
    31261 ctaatatgtt tatttccatc agtgctggaa ttgtgacatt tttactaact ttagtaggaa
    31321 ttccggcctt tatccaattt tatagaaagg cgcaaattac aggccagcag atgcatgagg
    31381 atgtcaaaca gcatcaggca aaagctggga ctcctacaat gggaggtttg gttttcttga
    31441 ttacttctgt tttggttgct ttctttttcg ccctatttag tagccaattc agcaataata
    31501 tgggaatgat tttgttcatc ttggtcttgt atggcttggt cggattttta gatgactttc
    31561 tcaaggtctt tcgtaaaatc aatgaggggc ttaatcctaa gcaaaaatta gctcttcagc
    31621 ttctaggtgg agttatcttc tatcttttct atgagcgcgg tggcgatatg ctttctgtct
    31681 ttggttatca agtgcatcta gggattttct atattgtctt cgctcttttc tggctagtcg
    31741 gtttttcaaa cgcagtaaac ttgacagacg gtgttgacgg tttagctagt atttccgttg
    31801 tgattagttt gtctgcctat ggagttattg cctatgtgca aggtcagatg gatattcttc
    31861 tagtgattct tgccatgatt ggtggtttgc tcagtttttt catctttaac cataagcctg
    31921 ctaaggtctt tatgggtgat gtgggaagtt tggccctagg tgggatgctg gcagctatct
    31981 ctatggctct ccaccaagaa tggactctct tgattatcgg aattgtatat gtttttgaaa
    32041 caacttctgt tatgatgcaa gtcagttatt tcaaactgac aggtggtaaa cgtattttcc
    32101 gtatgacgcc tgtacatcac cattttgagc ttgggggatt gtctggtaaa ggaaatcctt
    32161 ggagcgagtg gaaggttgac ttcttctttt ggggagttgg gcttctagca agtctcctga
    32221 cccttgcaat tttgtatttg atgtaagaat ggcaccctga tgtttcaggg tgtttttgtg
    32281 tttaaataca caatgaaaat caaagaacaa actagaaagc taactttagg ctgctcaaaa
    32341 tatagtagat tgaaactaga atagtacacc tctacttcta aaacattttt agaaatcgat
    32401 ttgactgtcc tgaacgattt atcctgttct tatttcattt tactatacag ttttgagatg
    32461 gttgatagaa ctgacgaagt cagctcaaaa cactgttttg aggttgtaga caaggcgaag
    32521 ctgatgtggt ttgaagaggt tttcgaagag tgtaaaaaaa tattggtcag ttaaagtcaa
    32581 aactcttgac cttttctgac caatgtagta tattatgaac gtaaggtaaa tgaaagcctt
    32641 actagaacac ttatttaaag taaaatagaa agaggtgagt ctatgtttca tctagcaatc
    32701 ttcagaggta aagatagtct actcctgctt gaaaaagaaa aaccagaaat agtacgtagg
    32761 gtagcgatct agctagtcta aattttttaa aacaatggtc aaagatagtc aatatcagta
    32821 atcataacta agtaaacaaa aagaggtaaa gaatatgaat aacaacttta ataattttaa
    32881 taacatggat gatttattta accaattgat gggtggtatg cgaggatata gttctgaaaa
    32941 tcgccgttac ttaattaatg gacgcgaagt cacacctgag gaatttgctc actatcgtgc
    33001 gactggtcaa ttaccaggaa atgcagaaac tgatgtgcaa atgccacaac aggcatcagg
    33061 tatgaaacaa gacggtgtcc ttgcaaaact aggtcgaaac ttaacagcag aagcgcgtga
    33121 gggcaagttg gatcctgtta tcggacgaaa caaggaaatt caagaaacat ctgaaatcct
    33181 ctcacgccgc accaagaaca atcctgtttt ggtcggagat gcaggtgttg gtaagacagc
    33241 agttgtcgaa ggtctagcgc aagccattgt gaacggagat gttcctgctg ctatcaagaa
    33301 caaggaaatt atttccattg atatctcagg tcttgaggct ggtactcaat accgtggtag
    33361 ctttgaagaa aatgtccaaa acttagtcaa tgaagtgaaa gaagcaggga atattatcct
    33421 cttctttgat gaaattcacc aaattcttgg tgctggtagc actggtggag acagtggttc
    33481 taaaggactt gcggatattc tcaagccagc tctctctcgt ggagaattga cagtgattgg
    33541 ggcaacaact caagacgaat accgtaacac catcttgaag aatgctgctc ttgctcgtcg
    33601 tttcaacgaa gtgaaggtca atgctccttc agcagagaat acttttaaaa ttcttcaagg
    33661 cattcgtgac ctctatcaac aacaccacaa tgtcatcttg ccagacgaag tcttgaaagc
    33721 agcggtggat tattctgttc aatacattcc tcaacgtagc ttgccagata aggctattga
    33781 ccttgtcgat gtaacggctg ctcacttggc ggctcaacat ccagtaacag atgtgcatgc
    33841 tgttgaacga gaaatcgaaa cggaaaaaga caagcaagaa aaagcagttg aagcagaaga
    33901 ttttgaagca gctctaaact ataaaacacg cattgcagaa ttggaaagga aaatcgaaaa
    33961 ccacacagaa gatatgaaag tgactgcaag tgtcaacgat gtggctgaat ctgtggaacg
    34021 aatgacaggt atcccagtat cgcaaatggg agcttcagat atcgaacgtt tgaaagatat
    34081 ggctcatcgc ttgcaagaaa aggtgattgg tcaagataag gccgtagaag ttgtagctcg
    34141 tgctatccgt cgtaaccgtg ctggttttga tgaaggaaat cgcccaatcg gcagcttcct
    34201 ctttgtaggg tctactgggg ttggtaagac ggagcttgct aagcaattgg cactcgatat
    34261 gtttggaacc caggatgcga ttatccgttt ggatatgtct gaatacagtg accgcacagc
    34321 tgtttctaag ctaattggta caacagcagg ctatgtgggt tatgatgaca atagcaatac
    34381 cttaacagaa cgtgttcgtc gcaatccata ctctatcatt ctcttggatg aaattgaaaa
    34441 ggctgatcct caagttatta cccttctcct ccaagttcta gatgatggtc gtttgacaga
    34501 tggtcaagga aatacagtaa acttcaagaa tactgtcgtt attgcgacct caaatgctgg
    34561 atttggctat gaagccaact tgacagaaga tgcggataaa ccagaattga tggaccgttt
    34621 gaaacccttc ttccgtccag aattcctcaa ccgctttaat gcagtcatcg agttctcaca
    34681 cttgactaag gaagaccttt ctaagattgt agatttgatg ttggctgaag ttaaccaaac
    34741 cttggctaag aaagacattg acttggtagt tagtcaagcg gctaaagatt atatcacaga
    34801 agaaggttac gacgaagtca tgggggttcg tcctctccgt cgcgtggttg aacaagaaat
    34861 tcgtgataag gtgacagact tccacttgga tcatttagat gctaaacatc tggaagcaga
    34921 tatggaagat ggcgttttgg ttattcgtga gaaagcctaa gatagaattt tgaggataaa
    34981 aaagaaggag ccagctgaaa aaaactggtt cctttttagg tacgacaggc atgtcgtata
    35041 gtagaagtgt attattccag tttcaatata ctatagtagc tcagaagtcg gtacttaaac
    35101 gtgctatatc aaaaccagtc cttgaaaaac gtggactggt ttcgtgtttg gattattacc
    35161 ttgaacgaca tgcgttaaaa gttagttgaa ccgccgtatg ccgaacggta cgtacggtgg
    35221 tgtgagaggg gctagagatt atcccctact cgattgtcta tagctatagg ggtaaagtta
    35281 ttagagaggt tgctagttta tagagacatc ttgatttgta aaaagttaaa ttttagacaa
    35341 ttatcccctt taaaaaatct attaaaccat agctagttga agtaaatttt tatagaacat
    35401 aattttattt atcaagacat tgtagagttg aggcaaattg aaaaagaaag tttaaaagtt
    35461 gataataagt caataagaaa gctattaatt ctaacgagat tatttgatag taatttgttt
    35521 tttcagctac tattcggaca atgttattat aataaacaca ggagagaaca ccagaaggta
    35581 gggacatcag aagaaatagt tcaggatata gttaacataa tcatcaaaga agctcctaga
    35641 ctcgctaatg ttaatttgga aacatattat ccagatgaat atatgtcaaa tacattgaag
    35701 caattactac tttttagaga ggagttcata aaaaatattt tgaatgggat tgatgtggat
    35761 gaaacagtag taatagaaaa tgattttgta gaagagttgg agaacttttt aaaagagaat
    35821 gatttgctag atttttcaaa tagcatattt tttcagttat ctggagataa agtaatagtg
    35881 aataatattt atccaggata tttagcatat tttaataggt tccttacatt ttatccgaaa
    35941 atattcgcta ttaaagaagt tggagaatat attgactttt taaattttga aaagagaatt
    36001 gcggatatgt tttattgttg gggatttaat gcaaattcta gagtgttgac agctgaaaaa
    36061 ataattgaaa tcccaaatca ccaaatcaat gctaagtaca taaaaaaagt tataaaaatg
    36121 agtgaactct tgtttggtgt tgatagtaat aacagaattc ggttgcttaa caatagaagg
    36181 gaattgataa aaactatttt ccaaggttct gtaattaagt cagcaattcc tgctctagct
    36241 gctactttgg atacaatcag taacagtgga tcatttcaaa tttctttatc aaagcagata
    36301 attaataatt tagtattaag tggtaaagat aaagttacaa ttcctaggat agagttagat
    36361 aatgttgttc tttcaagaca gaagattatt gtatcagttt cgtatataaa tgattttttg
    36421 gatttgagat ttccagataa aatagaatac attttaaatt ttttagaagg atctggtttc
    36481 aatactaaag taatggtcta ctttgggaaa attgtagaag aagataagaa ctattttgtt
    36541 aatcttgaaa aacctcagta ttttgatttt accagtatac ttttcaataa gttattcttt
    36601 aaagaattaa acaagtttga ttacatggtt attgaagaac tcattccaaa tcttgatgaa
    36661 gatgtaatga tgacagagta tataaaagag tatactgaat aggagcaatc tattatgatt
    36721 ttaagtaaag tacgttgtta catatataat tctaaaatta gaggtaaagt tctgtatgaa
    36781 ttatatgaat ttataaatga aaataagcaa ttgatggacg caattaattc aattaaagtt
    36841 gacagtagta atggttttag tattcaaata ttacatttag gagaacagca aacaactgtc
    36901 ttattaacag atttttttac taaaacttta gacttgttta aggaatttag ggttacaaat
    36961 aagagtactc ttgagcaggt gaaaaatcaa attagcatac taaattcttc tggtccaaaa
    37021 atggatttaa c
//
LOCUS       DAVBCB010000017        34347 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVBCB010000017 DAVBCB010000000
VERSION     DAVBCB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34347
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.017"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..4786
                     /locus_tag="VQU28_000809"
     CDS             <1..4786
                     /locus_tag="VQU28_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2476189.1"
                     /translation="QGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKG
                     AQGQAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRND
                     ADKTTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKG
                     EDGKTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTV
                     IFYYDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHT
                     ITITQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDE
                     LVQTTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTW
                     EPAGNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGDQ
                     GERGLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDG
                     KSLIAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNG
                     KNGVLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTT
                     TIRDGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIK
                     GPKGDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQG
                     KDGDKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHST
                     DITFTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGK
                     YTPGTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPL
                     AVIRDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQD
                     GQTPTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRI
                     RRDTDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITT
                     KDNGDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKE
                     TVVKNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPD
                     GSHTVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETT
                     VRDGKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVT
                     GADGKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPT
                     VTVKNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGT
                     VTKTVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVK
                     PVPAQPTPNVPTPEVPVQPTPAVPTPEVPVKPVPAVPEQPVVPTPAQPATPVNANPVA
                     PTTGKENRGDKLPETGSQSDYISVLLGSGILLSLYVGRRKEA"
     gene            complement(5023..5571)
                     /locus_tag="VQU28_000810"
     CDS             complement(5023..5571)
                     /locus_tag="VQU28_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2476190.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            5892..6380
                     /locus_tag="VQU28_000811"
     CDS             5892..6380
                     /locus_tag="VQU28_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HET2476191.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISASFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKIIFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            6377..7564
                     /locus_tag="VQU28_000812"
     CDS             6377..7564
                     /locus_tag="VQU28_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2476192.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            7557..7922
                     /locus_tag="VQU28_000813"
     CDS             7557..7922
                     /locus_tag="VQU28_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2476193.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            7938..9284
                     /gene="asnS"
                     /locus_tag="VQU28_000814"
     CDS             7938..9284
                     /gene="asnS"
                     /locus_tag="VQU28_000814"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HET2476194.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            9437..9727
                     /locus_tag="VQU28_000815"
     CDS             9437..9727
                     /locus_tag="VQU28_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HET2476195.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            9739..10209
                     /gene="ssbA"
                     /locus_tag="VQU28_000816"
     CDS             9739..10209
                     /gene="ssbA"
                     /locus_tag="VQU28_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HET2476196.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            10241..10480
                     /locus_tag="VQU28_000817"
     CDS             10241..10480
                     /locus_tag="VQU28_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HET2476197.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            10775..12175
                     /locus_tag="VQU28_000818"
     CDS             10775..12175
                     /locus_tag="VQU28_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HET2476198.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSYVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFDALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDVIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            12177..12536
                     /locus_tag="VQU28_000819"
     CDS             12177..12536
                     /locus_tag="VQU28_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HET2476199.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            12590..13339
                     /locus_tag="VQU28_000820"
     CDS             12590..13339
                     /locus_tag="VQU28_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HET2476200.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            13329..13628
                     /locus_tag="VQU28_000821"
     CDS             13329..13628
                     /locus_tag="VQU28_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HET2476201.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDMRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALFKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            13699..14634
                     /locus_tag="VQU28_000822"
     CDS             13699..14634
                     /locus_tag="VQU28_000822"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HET2476202.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            14721..15233
                     /locus_tag="VQU28_000823"
     CDS             14721..15233
                     /locus_tag="VQU28_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HET2476203.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGAVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            15270..15865
                     /locus_tag="VQU28_000824"
                     /pseudo
     CDS             15270..15865
                     /locus_tag="VQU28_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            15966..16160
                     /locus_tag="VQU28_000825"
     CDS             15966..16160
                     /locus_tag="VQU28_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HET2476204.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(16206..17651)
                     /locus_tag="VQU28_000826"
     CDS             complement(16206..17651)
                     /locus_tag="VQU28_000826"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HET2476205.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVDIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            17757..19379
                     /locus_tag="VQU28_000827"
     CDS             17757..19379
                     /locus_tag="VQU28_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2476206.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            19583..21541
                     /locus_tag="VQU28_000828"
     CDS             19583..21541
                     /locus_tag="VQU28_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2476207.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGFLQNLGLEPGEANDKAKAVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFATAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            21713..22648
                     /locus_tag="VQU28_000829"
     CDS             21713..22648
                     /locus_tag="VQU28_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476208.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILLLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIAVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTVYSRSKGSSVALENLLAVPKEKFE"
     gene            22673..23325
                     /locus_tag="VQU28_000830"
                     /pseudo
     CDS             22673..23325
                     /locus_tag="VQU28_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            23483..24577
                     /locus_tag="VQU28_000831"
     CDS             23483..24577
                     /locus_tag="VQU28_000831"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2476209.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            24585..25751
                     /locus_tag="VQU28_000832"
     CDS             24585..25751
                     /locus_tag="VQU28_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2476210.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEIQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDGLFEIDFDQLEKDLVEEE
                     VKLYILCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            25844..28942
                     /locus_tag="VQU28_000833"
     CDS             25844..28942
                     /locus_tag="VQU28_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2476211.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDSFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLNRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQNIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            28990..29607
                     /locus_tag="VQU28_000834"
     CDS             28990..29607
                     /locus_tag="VQU28_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HET2476212.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVGKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            29617..30951
                     /locus_tag="VQU28_000835"
     CDS             29617..30951
                     /locus_tag="VQU28_000835"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HET2476213.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFSGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            30961..31425
                     /locus_tag="VQU28_000836"
     CDS             30961..31425
                     /locus_tag="VQU28_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476214.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQYLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     DLDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            31422..31916
                     /locus_tag="VQU28_000837"
     CDS             31422..31916
                     /locus_tag="VQU28_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476215.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            31997..33646
                     /gene="mltG"
                     /locus_tag="VQU28_000838"
     CDS             31997..33646
                     /gene="mltG"
                     /locus_tag="VQU28_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HET2476216.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMTDSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDIIS
                     DTSRRSRREGAKPVNPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPIDA
                     NSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQKSM
                     STEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQAVGQLQGDFKESLTAEAFLA
                     KVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDEML
                     AAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDMPL
                     QSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIESSI
                     NQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            33717..34199
                     /gene="greA"
                     /locus_tag="VQU28_000839"
     CDS             33717..34199
                     /gene="greA"
                     /locus_tag="VQU28_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HET2476217.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
BASE COUNT        10715 a         6373 c         7776 g         9483 t
ORIGIN      
        1 ccaaggtgaa cgcggtttaa ctggagctaa aggtgaaaaa ggggaccaag gtgaacgcgg
       61 tctcactgga gcccaaggtg aaaaaggaga ccgaggcgaa cgtggtttaa ctggagctaa
      121 aggtgaaaaa ggagcccaag gtcaggcagg tcgtgacggt gtaactccaa ccgtaaccgt
      181 taaagataat aaaaatgacg gcactcatac tatcactatt aacgacggta gaggtaatgt
      241 tacaagtact gttgtaagag atggtttcga tggcgcaagt ccattagttg cgactcaacg
      301 aaatgacgca gataagacaa caactgttat cttctattac gataaaaatg gaaacaatga
      361 attagatgct tctgataaga aattaaaaga agttgtaatt gcagatggtg ctaaaggtga
      421 aaaaggggac aaaggtgaac aaggtcttca agggcgtgat ggtgaacaag gaccaaaagg
      481 agaagatggg aaaaccccaa cagttaaagt aactgatggt caagatggaa cgcatacaat
      541 tacaattaac gatggtaaag gtggtataac tactacagta gtaagagatg gatttgatgg
      601 tgcaagccct cttgtttcta ctcatagaaa tgaagcagat aaaacaacaa ctgttatttt
      661 ctattatgat ctaaatgata ataatcaatt tgatgaagga gatacaaaac ttaaagaagt
      721 tgttatcgca gatggaaaac aaggaccaaa aggtgacaaa ggtgataacg gaaaagatgg
      781 attcacacca gaagtaacag ttacagataa caataacgga acacacacca tcacaatcac
      841 acaaccagac aacagaccat cattaacaac aatcgttaaa aacggtgaag atggaaaaac
      901 accaaaagtc aaagcagaac gagatgatgc gaagaaacaa acaacattaa cattctacat
      961 tgacaaagat ggagatggaa gttacacagc aggaaaagac gagttagttc aaacaacagt
     1021 agttaaagac ggacaagacg gagctgcagg agcatctgga cgtgatggaa aagaagtatt
     1081 aaacggaaaa gtagacccaa caacagaagg taaagatgga gacacattcg taaatacaca
     1141 aacaggagat gtattcgtta agaaaggtaa cacttgggaa ccagcaggaa acatcaaagg
     1201 accgaaaggt gacaaaggtg cagatggtgc caagggtgaa aaaggagccc aaggagaacg
     1261 cggcctgact ggagcgcaag gtgtcaaggg tgaaaaggga gatcagggtg aacgcggtct
     1321 aactggatct aaaggtgaaa aaggagacca aggtgaacgc ggtttaactg gagcgcaagg
     1381 tgccaaaggt gacaaaggtg aacaaggtct tcaaggtcgt gatggagctc aaggaccaaa
     1441 aggcgcagat ggacaaagag gaccagcggg accacaagga ccaaaaggag aacaaggtaa
     1501 tccaggaact ccaggtaaag atggaaaatc tctaattgct gttaaaaatg gaactgaaac
     1561 aaaagtctat gtagaggatc cggctagacc aggacaacca cttaatccaa accaaccatt
     1621 agcaactatc actgatggaa gaaacggtac taatggacaa tcaccaacaa ttacagcgac
     1681 acgaagtgtg caaaatggca aaaatggtgt attagtaaca attacaccag taggaggtcg
     1741 tccacaaaca acatttgtag aggatggaca aaagggtgct gatgggaaaa ccccaacagt
     1801 aacaataact gaggggcaaa acggcacaca tacattaacg gttcataatc caggaagtcc
     1861 agatgtgaca actacgatcc gtgatggagc tacaggacaa gcaggtcgtg atggtaaaga
     1921 tgtattaaac ggaaaagtaa atccacaacc aaaccaaggt aaaaatggag ataaatatat
     1981 taataccgaa accggtgatg tctatgttaa aaacaatgga aactgggata aagaaggcaa
     2041 catcaaaggc cctaaagggg acaaaggtgc agatggtgct aaaggcgaaa aaggagacca
     2101 aggcgaacgc ggcctaactg gagcgcaagg agctaaaggt gcggatggcg cagtaggtcg
     2161 tgatggacgt gacggtaaag acgtgttgaa cggcaaagct aacccagaag cacatcaagg
     2221 taaagacggc gataaatacg ttaatacaga aacaggcgac gtcttcgtta agaataacgg
     2281 caactgggat aaagagggca acatcaaagg ccctaagggt gacaaaggtg aacgcggaga
     2341 agatggtaag actccagaag taactgtaac tccaggtaaa gatggccata gtactgacat
     2401 tacattcact gttccaggta aagatccagt tacagtaaat gttaaggacg gagaaaatgg
     2461 tctgaacggt aaaactccaa aagttgattt acttcgtgtc caaggaaaaa acggaaatcc
     2521 atctcataca attgtgacat tctatacaga tgaaaacaat gacggcaaat atacaccagg
     2581 aactgatgaa cttctaggtt cagaaatgat taaagatggt gctaaaggcg cggacggacg
     2641 agatggtaaa tcattgctta ctgtcaagga tggtaaagaa actaaagttt accaagaaga
     2701 tccagctaac ccaggacaac cattaaatcc agaaaaacca cttgcggtaa ttagagatgg
     2761 agtagatgga aaatcaccta cagttacagc tgttcgtaaa gatgaagcag ggcataaagg
     2821 tgtagaaatc actgttgata accatgatgg ttcacaacca actacagtct ttgttcaaga
     2881 tggtgctaag ggagaaactg gtgcaaccgg tcaggatgga caaactccta caatcactac
     2941 tcaacgtgga caagatggcc aaagcactgt tgtaactatc acaacatcag gtaaagatcc
     3001 agtaaccttc actgtaaaag atggtaagaa tggtaaagat ggccgtgcac cgaaaatcaa
     3061 agtagaagat attacttcac cttcaagaat tagacgcgat acagatgctg ctgcaactcc
     3121 aacgcgtaac ggtatccgtg ttacagttta tgatgatgtt aatgacaatg gggtatacga
     3181 cgaaggtgtc gataaagtat taaatagtaa agatatttat aacggtatag atggacgtga
     3241 tggttcagct ccaactatta ctacaaaaga taatggagat ggaactcaca ctatcacagt
     3301 tcaaaaccca gatggttctg aatcaacaac agttgttaaa gatggtaaag acggtaaaac
     3361 tgcgaatatc actacaacag aaaacccaga tggaagccac acaattacgg tgacaaatcc
     3421 agatggttca actaaagaaa ctgttgttaa aaacggtaaa gacggtaaga ctcctaaagt
     3481 tgaagtaacg gataacaacg atggaactca tactgttaaa gttacagatg gagacggcaa
     3541 tgttaccaac gctatcatca aagatggtaa agacggtaaa gctgcaacag caacaactac
     3601 tgaaaatcca gatggaagcc acacagtaac aatcactaac ccagacggaa ctaagaatga
     3661 gtttgttgtt aagaatggac gtgacggtgt tgacggacgt actccaaccg catctgttcg
     3721 tgataatgga gacggaagtc atacaatcgt tattacaaat ccagaaggtg tgacaactga
     3781 aaccacagtt cgtgatggta aatcaccaaa agtgactata actgatgaac aaaatggaac
     3841 tcataagatc tctgttctaa atggtgacgg aacaactaca acaactacag ttcgtgatgg
     3901 tagagaacca aaacttgaag ttattgataa caacgatggt tcacacacta ttaaagtgac
     3961 aggtgctgat ggtaaaggaa cgacaactac aatctttgat ggtaaatcac caaaagcgaa
     4021 catcgttgat aacggagatg gaactcatac attaacaatc gtagattctg atggtcgtga
     4081 atacaaatct attatcaaag atggtaaaga cggcaaagat agcgtttcac caactgtaac
     4141 tgttaaaaat aataacgatg gaactcacgt tgttacaatc actaatccag atggaagtaa
     4201 gacagaaatg gtgattaaag acggtaaaga tggtaaatca ccaaaagttt ctgttgaaga
     4261 taatggtgat ggtagtcata caatcacaat catcaattct gatggaactg tgacaaaaac
     4321 agttattaaa gatggtagag atggacgtga tggtcgagac ggcaaagacg gtaaagacgg
     4381 taaagatgga aaatgtggat gccaagacaa accagtaaca ccatcaaatg acaaaccagt
     4441 tcctccaaca ccaaatgtgc cgacaccaga agtaccggtt aaacctgtgc cagcgcaacc
     4501 aacaccaaat gtaccgacac cagaagtgcc agtacaacca actccagctg ttccaacacc
     4561 agaagtaccg gttaaaccag taccagcggt tccagaacaa ccagtagtac caacaccggc
     4621 tcaaccagca actccagtaa atgctaaccc agtagcacca actacaggta aagaaaaccg
     4681 tggggacaaa ttacctgaaa ctggaagcca atctgattat atctctgttc ttttaggtag
     4741 cggtattcta ttgagcctat atgtaggacg aagaaaagaa gcttaatcag ccaaaagcag
     4801 tcactaattt ctcgaaagaa gatgataagt gatagatctc gaatgaaaga atattcttag
     4861 aatgattttc ctatgatgga aaagactgct aaaaaccttg agtggtaacg ctcaaggttt
     4921 ttttggatgg ctgatgtttg tctgaaaatc ttgattgaga aataaaaagc caccctatta
     4981 ggtggctata attgatttag actttctaaa attttctatt ttttactgaa tcgcttcgac
     5041 caagtcttga gcttgttttt caagtgagtt taggactgtt tcttcaagaa ccaattttcc
     5101 gtctgcccag gcagagtcat taacacgtgc agcagtgaaa tcaccaacgc cttgtgtacg
     5161 gataaatggc aagaggtctt tgtagatagc gaaaagttga tcgtgccctg cattggctac
     5221 agatgatacg gtgacaaact tgtcttgaag ggcagaaacg ccacgcgtat cagacaagtc
     5281 aagggcacga gatagccagt caagcaagtt ttttactgta ccagggatag agaagttgta
     5341 gactggagag aaaatccaga tagcatccgc aacgagaact gcttcacgag cagcagctac
     5401 agctggatgt gttggaactt ccaaatcttg gctgaagaga ggaagggctg aataatcaag
     5461 gtagctaact tccgctttac cagcaagtgc tttctcagct tcgagcgcca tttggtggtt
     5521 gaaagaacct tgacgtagtg atccgacgat aaataatact tttttagaca tgatgtgtct
     5581 cctttttctt caaattttga gaactctctc gttgttataa aatacattac aacaaaacaa
     5641 aagaacttgc aagaaatttg ctcagagttt atttgaaaat tgtaaaattt cggaaactct
     5701 agtcatttgt gactgaaaaa gtaagaactg tttcactttc aaaatcggtt ttgtttgaag
     5761 ggagaaatag aattggttgc tcaaagcaca gctttgaggt tgcagataaa gctgacgtgg
     5821 tttgaagaga ttttcgaaga gtattcttat attcacttgt aataaagctt aatagatggt
     5881 aaaatagacg agtgaaaaaa agacaaaaca aagcaaaaaa taatctactg tggcagtatg
     5941 gtctagggat gacgattttg tttgtggtta tcagtgcttc ctttctgtat atggtttctc
     6001 ttagcatgaa accctatcaa acagctaaaa gtgaaggaga aaaattagct cagcagtatg
     6061 caggattaga gcaggccgat caggttgatt tatacaatgg cttggaatct tattacagcg
     6121 ttcttggtcg taataaacag caagaagcac ttgctgttct gattggaaaa gatgatcata
     6181 agatttacgt ttatcagcta aatcagggtg tttcacaaga aaaagcagaa acggtttcta
     6241 aggaaaaggg agctggcgaa attgacaaga ttatctttgg tcgttatcaa gataagccaa
     6301 tctgggaagt caagtcagga tctgattttt atctagtaga ttttgaaaca ggagcattgg
     6361 tcaacaagga gggcctatga aattatccaa ccgtgtttta gaaatggaag aaagtgtgac
     6421 tttggctgct ggagcccgtg ccaaagcact gaaggctgag ggtagagata ttttgtctct
     6481 aaccttgggt gagccagatt tcactactcc caaaaatatc caagatgccg ccattgcatc
     6541 gattcgagat gggcgtgctt ctttttatac agtaacctca ggtctgccag agcttaaggc
     6601 ggcggtcaat agctactttg agcgctttta cggctattct gtagcgtcaa atcaagtgac
     6661 agtcgctgcg ggagccaaat attctctcta taccttcttt atggctgtgg tcaatccagg
     6721 tgatgaagtc atcatcccaa ccccatactg ggttagctat ggagatcagg tcaagatggc
     6781 agaaggcgtt cccgtctttg tttctgctaa ggaagacaat cactttaagg tgaccgtaga
     6841 gcagttagaa gcagctcgca ctgacaagac caaggttttg gtgctgaatt cgccatctaa
     6901 tcccacaggt atgatttaca cccgtgagga actcttggca attggaaact gggctgtaga
     6961 aaatgatatt ctcatcctag cagatgatat ctatggccgc ttggtttata atggtcatga
     7021 gttcacaccg atttctagcc tatcggaagc gattcgcaag caaacagtgg tcatcaatgg
     7081 tgtgtctaaa acctatgcca tgactggttg gcggattggt tatgccgttg gagaagcaga
     7141 cattatcgct gccatgtcca agattgcagg tcaaacaact tcgaatccgt cagcagtagc
     7201 ccaatatgca gcagttgagg ctctatcagg tgagcaagat actgtagaaa gcatgcgtca
     7261 ggcctttgaa gaacgtctta ataccatcta tcccctcctt gcagaagtgc caggatttga
     7321 agtggtcaaa ccgcaagggg ccttctatct cttcccaaat gtcaaaaagg ccatggagat
     7381 gaaaggctac acggatgtga cagactttac aactgttatc ttagaagaag ccgaagtggc
     7441 cttggtaaca ggagctggat ttggagcacc agaaaatgtc cgcctcagct atgcgacaga
     7501 cctagacacg cttaaagaag cagtcgaacg cttgaaagca tttatgggta gtgagaatga
     7561 ttgatcattt tgagattaag gtaaaggatt tacaaatttc agaaggattt tataggagtt
     7621 ttctcgctcc tttggactat aaattggctt ttaagactag ttctctaatt agttttctat
     7681 ccccgaacag ccctcatcct ggtggtgatt tttggctgac tcagggaaca caagatcctg
     7741 ttcattttgc ttttttagca gaaaataagg aagaagttca ggcttgttat gaggctggct
     7801 tagaggcagg tgggcgagac aatggggctc ctggttatcg aagtgagcat ccgatttact
     7861 atgctgcttt tatgattgac ctggatggga acaatataga ggtggtttgc cataaagaat
     7921 aaaaaagaaa aggaaaaata atgacaaaac gtgtaacgat tattgacgta aaagactatg
     7981 ttggtcagga agtgacgatt ggcgcttggg ttgccaacaa atcaggaaaa gggaaaatcg
     8041 ctttcttaca attgcgtgat ggaacagcct tctttcaagg tgtggctttt aaaccaaact
     8101 ttgtcgaaaa atttggtgaa gaagtgggac ttgagaagtt tgatgttatc aaacgcttga
     8161 gccaagaaac gtctgtttat gtgacaggta ttgtcaaaga ggacgaacgt tctaaatttg
     8221 gctatgagtt ggacatcaca gacatcgaag tgatcggtga atctcaagac tacccaatca
     8281 caccaaaaga acacggaaca gactttttga tggataaccg tcacttgtgg ctacgctctc
     8341 gtaagcaagt agctgtgttg caaatccgta acgctattat ctatgcaact tatgagttct
     8401 ttgacaagaa cggttttatg aagtttgaca gcccaattct ttcaggaaat gcggcagaag
     8461 attctacaga actctttgaa actgactact tcggaacgcc agcctacttg agccaatcag
     8521 gtcaacttta cctagaagca ggggctatgg ctcttggtcg tgtctttgac tttggtccag
     8581 ttttccgtgc tgaaaaatca aaaacacgcc gtcacttgac tgagttctgg atgatggatg
     8641 ctgagtactc atacttgaca catgatgagt cgcttgactt gcaagaagct tatgtgaaag
     8701 ctcttctaca aggtgttctt gaccgcgcgc ctcaagcctt ggaaaccttg gaacgtgata
     8761 cagaactctt gaaacgctac attgcagagc cattcaaacg tatcacttac gatcaagcca
     8821 ttgacctctt gcaagagcat gaaaatgatg aagatgctga ctacgagcat cttgagcatg
     8881 gtgatgactt tgggtcacca cacgaaactt ggatttcaaa ccactttggt gtgccaacat
     8941 ttgtcatgaa ctatccagca gccatcaagg ccttctacat gaaaccagtt cctggaaatc
     9001 cagagcgcgt gctttgtgca gacttgcttg ctccagaagg ctatggagaa attatcggtg
     9061 ggtctatgcg tgaggaagat tacgatgccc ttgtcgctaa gatggatgaa cttggcatgg
     9121 atcgtacaga atatgaattc taccttgacc ttcgtaaata cggtacagtt ccacacggag
     9181 gatttggtat cggtatcgaa cgtatggtaa ccttcgcagc aggaacaaaa catatccgtg
     9241 aagctattcc attcccacgt atgttgcacc gtatcaaacc ataagaacag gaaaacgccc
     9301 atgtggcgtt ttttcgaatt ctagtgtttt tacttgcgta aaaaaaattt ttctagtata
     9361 atagtttatt gtgagcgaac ctcacttacc ccttgcaaag tcttggggtc attagaccaa
     9421 aaggaggaac atatcaatgg ctaaatacga aattctttat atcattcgtc caaacattga
     9481 agaagaagct aaaaacgctt tggtagcacg ttttgactct attttgactg acaacggtgc
     9541 aactgttgtt gaatcaaaaa cttgggaaaa acgtcgtctt gcatacgaaa tccaagattt
     9601 ccgtgaagga ctttaccaca tcgttaacgt tgaagcaaat gacgatgcag ctcttaaaga
     9661 gtttgaccgt ctttcaaaaa tcaacgctga cattcttcgt cacatgatcg tcaaaattga
     9721 cgcgtaagaa ggtaaactat gattaacaat gttgtacttg tagggcgtat gacacgtgac
     9781 gctgaattgc gttatacccc atcaaatgta gcagttgcga cttttactct tgcagtaaac
     9841 cgtacattta agagtcaaaa tggtgaacgt gaggctgatt ttatcaatgt cgttatgtgg
     9901 cgccaacagg ctgaaaatct tgctaactgg gctaaaaaag gctcacttat cggggtgaca
     9961 ggtcgtatcc agactcgtag ttacgataac cagcaaggac aacgtgtcta cgtgacagag
    10021 gtcgtggctg agaatttcca aatgttggaa agccgtagtg tgcgtgaggg tcacacaggt
    10081 ggagcttact ctgcaccaac tgcaaactat tcagcaccta caaattcagt accagacttt
    10141 tcacgtaatg aaaatccatt tggagcaaca aacccattgg atatttcaga tgatgattta
    10201 ccattctaat ggacaactaa acttataaag gagaaaaaac atggctcaac aacgtcgtgg
    10261 cggattcaaa cgccgtaaaa aagttgatta catcgcagca aacaaaattg aatatgttga
    10321 ttacaaagat actgagcttc ttagccgttt cgtttcagaa cgtgggaaaa tccttcctcg
    10381 tcgtgtaaca ggaacttcag ctaaaaacca acgtaaagta acaacagcta tcaaacgcgc
    10441 tcgcgtaatg gctttgatgc ctttcgtaaa cgaagattaa agaaaagacc cggttgggtc
    10501 tctttttcat gaacttgaaa acaagaaagc gaattaagac cccaacgggt cttttttcac
    10561 aagctttgaa atgagagagc gagttaggct cagtggctcc ctttttacga gtttgaaaac
    10621 aagagaacac attaaaagca cgatggagct ttttcataaa tatgtctttt ttttcatcca
    10681 agaaattaaa ataaaaatga taaacttttt gaaaataggg gatttgccta ctttatgata
    10741 taatggaaag agttagattg aaagaggtag tgagatggac gcgaaattaa gatacaaggc
    10801 aaagaagatc aagattgtct tttttgatat tgatgataca ttgcggaatt caaagacagg
    10861 gtttattcca actacaattc ccactgtttt taaacagttg cgtgaaaaag gaattttaac
    10921 aggaatcgcc tctggacgtg gcatttttgg tgttgttcca gagattcgtg atctcaaacc
    10981 tgactttttt gtaactttga atggggctta tatcgaagat aaaaaaggtc aggtcattta
    11041 tcagcatcag attgaaaagt catatgttga ggagtatatc tcttgggcta agcaagaagg
    11101 aattgagtat ggcttggttg ggagtcatga tgccaagttg tcgactcgca ccgatatgat
    11161 gagtgaagct atcaatccaa tttatcccga cttagatgta gatcccgatt tccatgaaaa
    11221 agaagatatc tatcagatgt ggacttttga agataaggga gatgacttgc acttgcctga
    11281 cagtctctca gacaaacttc gcatggttcg ttggcatcaa cattcgtctg atattgtgcc
    11341 gatttcaggc tccaaagcga cgggggtgga aaaggttgtg gaacaccttg gcttgaaacc
    11401 agagaaggtc atggtttttg gagatggtct caatgacttg gaactctttg attatgctgg
    11461 aatcagcgtt gccatgggaa tttctcatga taaaatcaaa gaaaaagcag attatattac
    11521 aaaaacatta gaagaagatg gcatttttga tgccttagaa gtatttggta tggtagaaaa
    11581 agaattgcat tttccacaag tagacattga aacagtagaa ggtcctcttg cgactattaa
    11641 gaccaatcac ggagacttac gtatcaagct tttccctgaa catgctccta aaacagtggc
    11701 taactttgta tctctttcaa aagatggcta ctatgatggt gtcattttcc accgtattat
    11761 caaggacttt atgatccaag gtggagaccc aactggaact ggtatgggtg gcgagtcaat
    11821 ctacggcgaa tcttttgagg atgaattctc agaagagctt tacaatatcc gtggtgctct
    11881 ttccatggca aatgctggtc caaataccaa cggcagccag ttctttatcg tgcaaaacca
    11941 acacctacct tattctaaga aagaaattac tcgtggtggt tggccagaac cgattgcaga
    12001 aatctatgcc aatcaaggtg ggacacctca cctagaccgc cgtcacacgg tttttggtca
    12061 gttagctgat gaagcatctt acgctgtctt ggatgtcatt gctgctgttg agacaggagc
    12121 tatggacaag ccagttgaag atgttgtaat tgaaactatt gaaatcgagg actaagatga
    12181 aaatcggtga taagctaaag gggcgtatta cagggattca gccctacggt gcctttgttg
    12241 agttagagac gggtgatacg gggctgattc acatttcaga aattcggaca ggatttattg
    12301 aaaatattca tgaaaccttg aaagtcgatg aagaggttca agttcaggta gtggatttag
    12361 atgaatttac ggggaaagcc agtctttcta tccgcacttt ggaggaagaa aagtaccagt
    12421 ttcctagacg gagacgcttt tcaagcgacc gctttaacta cggttttgct ccctttcgac
    12481 gaatgctacc aatctggact ggagaagccc tgcaccattt aaaaaagaag aagtagttta
    12541 gagttttata ttttttgtaa tgttaatata aatttgctat aataggacca tggaagaaga
    12601 acaattatta aaatcaggag agcgcattaa ccagctcttt tcgacagata tcaaaatcat
    12661 tcaaaataga gaggttttta gctattcggt ggatagtgtt ctcttatcac gatttccacg
    12721 ttttcctaag aaggggttga ttgtggattt ctgtgctggg aatggagcag tggggctttt
    12781 tgctagcact cgtactcaag cacagatatt gtctgttgag attcaggagc gtttggcgga
    12841 tatggctgaa cgctctgtcc gtttgaatgg attagaggag caaatgcagg tcatctgcga
    12901 tgatttgaaa aatatgcctg ctcacattca ggggagtaag gtggatatga ttttgtgtaa
    12961 tccaccctat ttcaaggtgg atccttattc taatctgaac gagagtgaac attatctctt
    13021 ggctcgacac gaaatcacga ctaatttgga agaaatctgt catagtgccc agagtattct
    13081 caagtctaat gggcgtttgg ccatggttca tcgtcctgat cgacttctgg atattttgga
    13141 tacgttaaaa cgacataatc tagcccctaa gcgcctgcag tttgtttatc caaaaagaga
    13201 aaaggaagcc aatatgcttt tgattgaggc gatcaaggat ggctcaacaa gtggctttaa
    13261 ggtcttacct cctttgattg tccacaatga tgatggctct tatacgcccg aaatcgaaga
    13321 gatttattat ggatcataag gcttatatgt acgttctgga gtgtcgtgat ggatcctact
    13381 atataggcta tacgactgat atgagaagac gcctcgctat ccacaatagt gggaagggag
    13441 ccaaatatac acgagcacgc ttgccagtca aacttatcta tgctcaaggt tttgccagta
    13501 aggaagaagc catgtcggct gaagctcttt tcaagcgtaa gaagaggcca cagaaggaag
    13561 aatttttatc tgaaaatcaa gatagaaatt tactcagtta ttttgaagaa tcgtggggag
    13621 tcctttgact cctcttacct ttgtcaaaaa gcgaaaaaaa tgctacaata aagaaaataa
    13681 acaaaatgag gtattatcat gtccaagatt ctagtatttg gtcaccaaaa tccagactca
    13741 gatgccatcg ggtcatctgt agcttttgcc taccttgcaa aagaagctta cggtttggat
    13801 acggaagctg ttgcccttgg aactccaaat gaagaaacag cctttgtctt gaactatttt
    13861 ggtgtggaag caccacgtgt tatcacttct gccaaagcag agggggcaga gcaagttatc
    13921 ttgactgacc acaatgaatt ccaacaatct gtatcagata tcgctgaagt agaagtttac
    13981 ggtgttgtag accaccaccg tgtggctaac tttgaaactg caagcccact ttacatgcgt
    14041 ttggagccag ttggatcagc gtcttcaatc gtttaccgta tgttcaaaga acatggtgta
    14101 gctgtgccta aagagattgc aggtttgatg ctttcaggtt tgatttcaga tacccttctt
    14161 ttgaaatcac caacaacaca cccaacagat aaaatcattg ctcctgaatt ggctgaattg
    14221 gctggtgtaa acttggaaga atatggtttg gcaatgttga aagctggtac caacttggct
    14281 agcaaatctg ctgaagaatt gattgacatc gatgctaaga cttttgaact caacggaaat
    14341 aatgtccgtg ttgcccaagt gaacacagtt gacatcgctg aagttttgga acgccaagca
    14401 gaaattgaag ctgcaatgca agctgccaac gaatcaaacg gctactctga ctttgtcttg
    14461 atgattacag atatcgtcaa ctcaaactca gaaatattgg ctcttggtgc caatatggac
    14521 aaggtcgaag cggctttcaa tttcaaactt gaaaacaatc atgccttcct tgctggtgcc
    14581 gtttcacgta agaaacaagt ggtacctcaa ttaactgaaa gctttaatgc gtaagatttt
    14641 gggtgtcagc tcaaaatcgg aaagtctagt ttgccttata tcgaaaggag tttcggctcc
    14701 tttttttcta ggagtgaagt atgttagaaa atggcgattt gatttttgtg agagatgggt
    14761 cagacatggg acaggccatc cagacttcca caggtaacta tagccatgtt gccatttatt
    14821 tggatgggat gatttatcat gctagtggac aggctggtgc tgtctgtcaa gaaccggcag
    14881 acttctttga gtccaatcat ttgtacgacc tctatgttta cccagaaatg gatatccagt
    14941 cggtgaagga aagagcttgc agacatcttg gagcacccta caatgcttct ttctatccag
    15001 atgcagctgg tttctactgc tcccagtata tagcagaaat cctacctatt tttgaaacta
    15061 ttcctatgaa atttggagat ggggaacagg agattagtga tttttggagg gagtattaca
    15121 tagaactagg tctgcctgtt cctctgaacc aagctgggac caatcctagt cagttggcag
    15181 catcgcctct gttacaatgt aaagaaagga atcttcatga ttcagatttt taattcatct
    15241 cgtttgacga gacagccatt tttggagaat tgatccgcta tctggaccag tatgaggatg
    15301 tgattctacg ggaaattaag gctcaatttc cagatgttgc agttgataaa ctcatggaag
    15361 agtatataaa ggcaagcttg attctacgtg aaaataagcg ctattacctc aattttccta
    15421 cgcttgaatc acttgatagt cttgaactgg atcaagagat ttttgtcaga gaagctagtc
    15481 cggtctatca agccttgttg gagcagagtt ttgagacgga attgcgcaat caaatcaatg
    15541 cagctatttt agttgaaaga cggactttgc gcgcattaaa atgaccctgt ccaattattt
    15601 ttacaaggtc aaacagcagt atcctttgac agaaaaacag caggagctct atgacatttt
    15661 aggagatgtt aatcctgagt atgcactcaa gtatatgacg gcttttttgt tgaaatttct
    15721 caaaaaagac cagcttatgc agaaatgccg tgatatcttt gtggacagtt tggttgtctt
    15781 aggctatatt gtgcaaaatg aagatagaaa gtatgagttg gctatcgatt ttgataagga
    15841 gagattaact ttctacttag cgtgatttct tgtttctgag cacattgttt gactttcctt
    15901 agtattcggt ataaactata tgtaaccggt aacacatatc ggaataaact aaaggagaca
    15961 atcatatgtc acttgaaaac aaattggaac aagcaacagg cgctgtcaaa gaaggttttg
    16021 gtaaagttac tggagacagc aagacagaac ttgaaggagc tgttgaaaaa acagttgcta
    16081 aggcaaaaga cgttgtagaa gacgcaaaag gtgctgtaga aggtgccgtt gaaggtttga
    16141 aaaacgtttt tactaaataa taggaaaaaa tcaagggttt cattttccct tgattttttc
    16201 tattcttata aataattttc tgcgacggct gtatctcctg ggtaggattc tttcttgccc
    16261 tggatgattt ggtaacaatc ggctccctta cccgcaataa taactgcatc taattcgtga
    16321 tttgtgatag ccattgccgc cttgatggct tcttggcgat ccgcaatctt ttcaacagga
    16381 tgattgatgt agctactaat ttcatctgca atggccattg ggtcttcata gttagggtca
    16441 tcagcagtca gaaagacttg aatctcaggg tgttgattga ggaggaggcc aaagtcctta
    16501 cgacgacttt ctcccttgtt tcctgttgat cccagaacca gagcaatctt tccggtttga
    16561 tgagtttcaa ccacattgat gagttttttc agactatccc cattgtgggc atagtcgatg
    16621 aagaccttgg ctccattttt ctgagtgagg acttccatac gaccaggaac gcgggttgca
    16681 gcgatgcctt ttttgatgtc ctcaagactt gctccgagac ggagacaagc aagtccagca
    16741 gcaactgcat tttcttggtt gaagttgcca atgagttgga tatcataatc tccagcgagt
    16801 ttacccgtag ctgaaaagct aaaggctttg gaattctcga tttggttatc aaattggcta
    16861 ccatagaaat catggtcttg atcttcaacc tgttctttca agactgagaa gtggtccatg
    16921 tcactgttaa tgatgactgc tcggctattt tccatcaaga gacgcttgtg gtagaaatag
    16981 tcttcaaagc tagggtgttc aatcgggccg atatggtcag gagtgatgtt aagaaagact
    17041 cctacatcaa aggtcagtcc atagactcga tggactagat aggcttgact ggagacttcc
    17101 atgatgaggt gggtacggtc attttgcaca gcctgattca tcatgtcaaa gaggtcaata
    17161 ctctcagggg ttgtcaacgc tgacttaaag aaagtctcgc catcaagagt tgtgttcatg
    17221 gtcgacaaca tagcaggtct atgcccttga gataagatgt tataggcgaa ataggttgct
    17281 gttgtcttac ccttagtacc agtaaaggca aggagtttga gtttctcttg tggattacca
    17341 tagaactcca tggcaatcaa actcatggct ttctttatat cgttcacaat gatgacaggg
    17401 atatcgactt cgtagtcctt ttcagctaca taccaagcta aaccttgtgt tatagcagaa
    17461 agaaggtatt cttttttaaa ggcagcgcct tttgcaaaaa aaagagtgtc ttctgttact
    17521 tttcggctgt cgtagctgat gctatcaaaa ataactttgc tgtagttgta gtggtaatga
    17581 ccttggtcaa taatttcgcg aaaaaggcca tctttcttta aaatatctaa tacggtttca
    17641 atcttaatca tactttctat tgtaaaccga aagtcgtaaa tttacaagta acaaggaaaa
    17701 gtttataatg gaagataagg agtttttcct agttatcaaa attgaatgag gaatctatgt
    17761 cgcacgaaaa caatcaccag caggcccaga tgttacgggg gactgcttgg ctaacggcta
    17821 gtaactttat cagtcgccta ctcggggctg tttacattat cccttggtac atctggatgg
    17881 gggcttatgc agctaaggca aatggtctct ttaccatggg ttacaatatc tatgcttggt
    17941 tcttgttggt ttcaacagcg gggattccag ttgcggtggc caagcaagtt gccaagtata
    18001 ataccatgcg agaagaagag catagctttg ccctgattcg gagcttctta ggctttatga
    18061 caggactagg cctggttttt gctttagtct tgtatgtctt tgctccttgg ctagcagact
    18121 tgtctggcgt gggcaaagac ttgatcccaa tcatgcaaag cttggcttgg ggagtcttga
    18181 ttttcccgtc tatgagtgtt atccgaggat ttttccaagg gatgaataac ctcaaaccct
    18241 atgccatgag ccaaattgct gagcaggtca ttcgtgttat ctggatgctc ctagcaacct
    18301 ttatcattat gaagctcggt tcaggagatt atctagcagc cgttacccaa tcaacctttg
    18361 ctgcctttgt cggtatggta gccagttttg cagtcttgat ttatttcctt gcccaagaag
    18421 gttcactcaa aagagtcttt gaaacaggag ataagattaa cagtaagcgt ctcttggttg
    18481 ataccattaa ggaagccatt ccttttatcc tgacagggtc tgccatccag ctcttccaga
    18541 ttttggatca gctgaccttt atcaatagta tgagctggtt taccaactac agcaatgagg
    18601 acttggttgt catgttttct tatttctcag ccaatcctaa taaaatcacg atgattttga
    18661 tttctgtagg ggtttcgatt gggagtgttg gtttgccact tttgacggaa aactatgtca
    18721 agggggactt gaaagcagct tctcgtctcg ttcaggacag tctcacccta ctctttatgt
    18781 tcttgctacc agcaacggtt ggagtggtta tggtaggaga acctctttat acggtcttct
    18841 atggtaagcc agatagtttg gctctgggct tatttgtctt tgcagttttg cagtctatta
    18901 ttttaggctt gtacatggtc ttgtctccaa tgcttcaggc catgttccgc aaccgcaagg
    18961 ccgttctcta ttttatctat ggttctattg ccaagctagt cttgcaacta cctaccatcg
    19021 ccctcttcca cagttatggt cctttgattt caacaaccat tgctctcatc attcctaacg
    19081 tcttgatgta tcgggatatt tgtaaagtaa ctggtgtcaa gcgcaaggtg attttgaagc
    19141 gaaccatttt aatcagtttg ctgaccctag tcatgtttct gttaatagga accatccagt
    19201 ggctgttagg atttttcttc caaccaagtg gacgtttgtg gagcttcttt tatgtagctc
    19261 ttgtcggtgc catggggggt ggactttata tggttatgag tctgcgtacc tatttattag
    19321 ataaggtaat aggaaaagcc caagcagatc gcctgcgagc aaaatttaag ctttcgtaag
    19381 gtagcattta taaataaaag gaataagttg aacaatcttt taggagaact tgaagcttat
    19441 tcctttttta gcgtaaaaat agctaaaatt atatttcaaa aatagaaaat gaggcttttt
    19501 tcgatttttt ggaaaaaatc attgactaaa caaactaaac gttgtataat aaaacaaata
    19561 tttaaagcag gaggttccgg aaatgaaaaa gtctaagagc aaatatctaa ccttggcagg
    19621 tcttgtcctg ggtacaggag ttttattgag cgcgtgtgga aattctagca cggcgtcaaa
    19681 aacatacaac tatgtttatt caagtgatcc atctagcttg aactatctag cagaaaaccg
    19741 cgcagcaaca tccgatattg ttgcaaattt ggtagacggg ttattagaaa atgaccaata
    19801 tgggaatatt attccatcat tagcagagga ttggactgtt tctcaggacg gtttgaccta
    19861 tacctacaaa cttcgtaagg atgccaagtg gtttacttct gagggagaag aatatgcgcc
    19921 tgtaactgcc caggattttg tgacaggttt gcaatatgca gctgataaaa aatcagaagc
    19981 cttgtatcta gtgcaggact ctgttgctgg tttggatgac tatatcactg gtaaaacaag
    20041 cgacttttca actgtcggtg tcaaggcact tgatgaccaa acggttcaat atactttggt
    20101 taaaccagaa ctttactgga attcaaaaac acttgcaacg atactttttc ctgttaatgc
    20161 agatttcctg aaatcaaaag gggatgattt tgggaaggcg gatccatcta gtattttgta
    20221 caatggacct ttcttgatga aagcacttgt ctcaaaatct gctattgaat ataagaaaaa
    20281 ccctaattac tgggatgcta agaatgtctt tgtagacgat gtgaaattga cctactatga
    20341 tggtagcgac caagaatcac tggaacgtaa ttttacagct ggtgcttata ctacggctcg
    20401 tctttttcct aacagctcca gctatgaagg gattaaagaa aaatacaaaa acaatatcat
    20461 ctatagtatg caaaattcaa cttcatattt ctttaatttt aacctagata ggaagtctta
    20521 caattatact tctaaaacaa gtgacattga aaagaaatcg actcaggaag cagttctcaa
    20581 taaaaacttc cgtcaggcta tcaattttgc ttttgacaga acatcttatg gggctcagtc
    20641 tgaagggaaa gaaggtgcaa caaagatttt gcgtaaccta gtggttcctc caaactttgt
    20701 cagtatcaag ggaaaagact ttggtgaagt tgtagcctct aagatggtca actatggtaa
    20761 ggaatggcaa ggtatcaact ttgcggatgg tcaagaccct tactacaatc ctgagaaagc
    20821 caaggctaag tttgcggaag ctaagaaaga actcgaagca aagggtgttc aattcccaat
    20881 ccacttggat aagactgtgg aagtaacaga taaagtaggc atacaaggag ttagttctat
    20941 caaacaatca attgaatctg ttttaggttc tgataatgta gtgattgaca ttcagcaatt
    21001 aacatcagat gagtttgaca gttcaggcta ctttgctcaa acagctgctc agaaagacta
    21061 tgatttatat catggcggtt ggggacctga ttatcaagac ccgtcaacct atctcgatat
    21121 ttttaatact aatagtggag gatttctgca aaatcttgga ctagagcctg gtgaggccaa
    21181 tgacaaggct aaggcagttg gactggatgt ctatactcaa atgttggaag aagctaataa
    21241 agagcaagat ccagccaaac gttatgagaa atatgctgat attcaagctt ggttgattga
    21301 tagttcttta gttcttccaa gtgtttcgcg tgggggaaca ccatcattga gaagaaccgt
    21361 accatttgct actgcctatg gtttaaccgg tacaaaaggg gttgaatcat ataaatacct
    21421 caaagtacaa gataagattg tcacaacaga cgaatatgca aaagccagag aaaaatggtt
    21481 gaaagaaaaa gaagaatcca ataaaaaagc ccaagaagaa ttggcaaaac atgtcaaata
    21541 aaatctaatt gtagataagt ttgtgtaaga agtctttgtg cttaaaaacg cataatatca
    21601 ggtatataaa atcctagatg ttatgcgttt tgttatagga agatttatta gattttatcc
    21661 tcaaattgag ttattcccga ctttatccgt tgaaatatta taaggagtat ttatgaaatt
    21721 aaaattatta agagtagata ctaaggtgat tatggggagt ttcttacttg ttctgtctag
    21781 tctacttgct ttgttgcttc cccttatctt aaagggttta atagatggga gttctattga
    21841 aaatataggt tccaaagtat ttcaatcgtt tttgattttt attggtcaag ccttgttttc
    21901 ttctattggt tactatctgt ttagtcaatc gggtgaaaaa aagatagcaa aaatcaggaa
    21961 aaaagtgata gaggggttga tttatgcaga gaaatccttc tttgataaga gccaaagtgg
    22021 ggagttgact tctgccattg tcaatgacac gagtgtcatt cgtgagtttt taattacgac
    22081 tttcccaaat attattctga gtttagttat ggtacttggt tccattgtag tcttatttag
    22141 tcttgattgg aatctttctc tacttttatt catcactctt ccttgtatga tgtttattat
    22201 cttgctcctt tccaatatca gtgaaaagta tagtcgtcgt ttacaggagg aaatcggatt
    22261 tttaacaggg caattgactg aaaagattca agaacatgaa ttaattaaga ctaaccaagc
    22321 agagaaaagt gtacaggatg ttttggataa ttgtattgaa agggtacaaa acaattcact
    22381 gaaatcagat agggttactt cttttgagac cccgtttgct ctcttattta tctttgcgac
    22441 tatagctgtg atgctaacct atgggggtta tcgggtcagc gcaggatata tatctgtggg
    22501 aaccttggtt tcgtttttga tttacctctt tcaattactt aatcctatta gtaatatagc
    22561 taattttgta actgtttatt ctaggagcaa gggatcttca gttgcactgg agaacttgct
    22621 tgcagttcct aaagaaaaat ttgagtgagg aaaatcggta tcaggacaag ggttgaattt
    22681 taaccatgtc tattttggtt atgatgaaaa tcgacctgtc ttaaaggata ttacttgttc
    22741 aattttcaag gggcaaaaaa ttgcttttgt tggaccatct ggatcaggaa aatcaacgat
    22801 tgtgcgtttg ttagagcggt tttataaacc gctttcagga gatattctaa tggagcaatc
    22861 aagtatatat gattttaact taaaagaatg gagaagtaaa atcgcttagt aactgatgat
    22921 gaattgatga aagtgctaga cttagtatca ctaggtgatg agattcgctc catgaaagag
    22981 ggactagata ctgaagttgg tgaacgcgga cgactcttgt caggggggca aagccaaaga
    23041 cttcaaatag ctagagccta cttaaaagat gctgaaattc ttatatttga tgaagccact
    23101 gctaatcttg atgcggattc tgagtatgcg attatcagta gcctctattc tgtattaaag
    23161 gagaagacgg ttgtgattat agcgcatcgt ttgtcaacgg taaaagatgt ggattgtatt
    23221 ttcttcttag aggaggggaa aatcactggc tcaggaactc ataaggaact actggaaaat
    23281 catgagcgtt atgctcgttt tgtgcaggag caaatgatag agtgaaggtg tcttttgaga
    23341 ttcaccattt tatagtctat taaagggagc aggaaaaact ccctttttat atagtttgaa
    23401 actataacta gctcttgaaa agaagaaaat gagttgatga aaataagtgg tacaatagtt
    23461 actatagatt tggaggtatt gtatgagcaa ggaattacac attaacacaa ttttggccca
    23521 ggcgggtatt aagtcagatg aagcgacagg tgcattggtg acaccgcttc atttttcaac
    23581 gacctatcag catccagagt ttggtcgatc tactgggttt gactatacgc gcactaaaaa
    23641 tccaactcgt agtaaggctg aggaagtctt ggcggctatt gagtcagcag actatgccct
    23701 agcgactagc tcagggatgt cagctattgt actggccttt agcgtctttc cagtaggaag
    23761 taaggtcttg gcagtgcgtg atctttacgg tggttctttt cgctggttta accaagtgga
    23821 gcaggaaggt cgtttccatt ttacctatgc caatacagaa gaagagttga ttgccgagtt
    23881 agaaaaggat gtggatgttc tctatatcga aaccccaacc aatcccttga tgttggaatt
    23941 tgatatcgaa aaactagcaa aattggctca tgctaagggt gccaaagtgg tggtggacaa
    24001 taccttctat agccctatct accaacgtcc gattgaagat ggagcagata tcgttctcca
    24061 ttcagcaacc aagtatctag caggccacaa tgatgtcttg gctggagtgg ttgtgaccaa
    24121 tagtttagaa ctatacgaga agctttttta caatctcaat acaacagggg cagtcttgtc
    24181 tccatttgac agctaccagt tgcttcgtgg tctcaagacc ttatctcttc gtatggagcg
    24241 ctcaacagct aacgcccaag aagtggttgc ctttttgaag gattctccag cagttaagga
    24301 agttctctac actggtcgtg gaggcatgat ttcctttaaa gtagccgatg aaacacgcat
    24361 tcctcatatt ttgaacagtc tcaaggtctt ctcttttgcg gaaagtttgg gcggagtgga
    24421 aagtcttatt acttatccaa cgactcaaac tcatgctgat attccagcag aagtacgcca
    24481 ttcttatggt ttgacagatg acctcttgcg tttgtctatt gggattgagg atgctagaga
    24541 tttgattgca gatttgcgcc aagccttaga aggataagac aaagatggga aaatatgatt
    24601 ttacaagcct gcccaaccgt ttagggcacc atacctataa atggaaagaa acagaaacgg
    24661 atagtgaagt tctaccagct tggatagcgg atatggactt tgtggtcttg cctgaaatcc
    24721 gccaagccgt gcaaacttac gcagaccaac tggtttatgg ttatacctat gccagtgaag
    24781 acttaattaa ggaaattcaa aagtgggaag ctacacaata cggttacaac tttgacaaag
    24841 aggctcttgt ctttatcgag ggtgtggtac cagccatctc aacagctatt caaaccttta
    24901 caaaagaagg cgaggcggtt ttaattaaca cgcctgtcta cccacccttt gctcgcagtg
    24961 tcaagttgaa taatcgtaga ttgattacta attccttagt ggaaaaggat ggtctgtttg
    25021 agattgactt tgaccaactt gaaaaggatt tggtggaaga ggaggttaaa ctctatattc
    25081 tttgcaaccc tcacaatcct ggtggacgtg tttgggaaaa agaagtgttg gagaagattg
    25141 gccaactttg ccaaaaacac ggtgttttgt tagtttcgga tgagattcac caagatttga
    25201 ccctctttgg tcacaaacac cagtctttca ataccatcaa tcctgccttc aaaaattttg
    25261 ctatcgtctt gagcagtgcc actaaaacat ttaatattgc tggaacaaaa aattcctatg
    25321 cagtcattga aaatcctaag ttgagactgg ctttccagaa acgcctgttg gccaataatc
    25381 agcatgaaat ttcaggcttg ggttatttgg cgacagaagc tgcctatcga tacggtaaag
    25441 attggctaga ggaactcaag caagtctttg aagaccacat caattatgtg gtggatctat
    25501 ttggaaaaga gactaaaatc aaggtcatga aaccgcaagg tacctacttg atttggcttg
    25561 acttttcagc ctatgacctg actgatgaaa cattgcaaga gttgttgaga aatgaagcca
    25621 aggttatcct caaccgtggt ttggattttg gagaggaagg aagtctccat gcccgcatca
    25681 atatagctat gcccaaatct ctgttgcaag aagtctgtca gcggattgtg gctacttttg
    25741 ccaaacgtta aaaatccagc cttctaggag aaaagtcttc ctagaaggct attttcatag
    25801 gcgaaaatat ggtataataa aaagataagg taaaggtgaa aatatggcta aattgattcc
    25861 ggggaaaatt cgtatcgaag gtgttgccct ttatgaaact ggtaaggttg atatcatcaa
    25921 ggaaaagaac aatcggctct acgctcgcgt tgcagaagaa gaactgcgct atagtttaga
    25981 ggatgatttg gttttttgtg cctgtgattc ttttcaaaag aggggctact gtgtgcattt
    26041 ggcagcgcta gagcattttc tgaaaaatga tgagcgtggt caggaaatct tgtggagtct
    26101 ggaagaaggt catgaagaaa aagaggccgt tgaaaccaag gtgaccttgg gtggcaagtt
    26161 tttgaatcga attttatctc caaaatcaga atgcgcctat gagttatcag ctgtgggtca
    26221 ggtggaagca ggaaccaatc atatcttgtg gactctgaga atcggccaaa tcaatagcca
    26281 aaaatactat gtcattcggg atattccact ttttttaagg attgtcgagc agagaaagtc
    26341 gtatatgatt ggcaagattt atgaggagtc tctttcttgg gaagcctttg atgatgccag
    26401 ccaagaactt ttgactttct tgcacggact aacggaggaa gggctggttc cagacctctt
    26461 tttccaaaac caaggtcggc acctcttttt ccctctgacc ttttttgagc agggtgtgaa
    26521 tttactgatg gccttgcccc attttcaatt tgaccatcaa gtggatagct accaaaccct
    26581 gctttttcag gatatgcatg ctgatgccaa tctatttgcc tttacagtaa cagaatactc
    26641 ggactatttt gaaatggaaa tcagtgagag tcctagggtc aatgtctttt atcagggagc
    26701 agtacttttc cataaaggtc aggtttattt tttaacagac cagcagatgc gtcttctcaa
    26761 ggaaattaaa gcgttacctg tgggtcaaca aggaaagaaa tacctgcaat ttgatagcag
    26821 tgatcgagac aaattggcct cctgtctgac tctttttatc cagatgggca ccgtttcagc
    26881 tccagaacgt ctacaaatca aaacttttgc gccctctttt tactttgaca gggaagaaga
    26941 taatcggatt cgtttagaaa ttcagtttga ttatggaaat agacaggtat ctagccgaca
    27001 agagctagaa gaattaccat tttcaagtga cgcggactta gaagaaagaa ttttccaagt
    27061 ctgtttggct gcgggctttg aagccgattt tcaatcttgg cgtcaggcct taaaagcgga
    27121 gtctgtctat catttttttc atgatatcat tccagtcttt gaaaaactcg ggcatgttga
    27181 cctatcagat aaactagaag agctttatag cctagcgagt cctcaggtgc agattgcctc
    27241 caagggaggt ctcttggaaa tccagtttga ttttcaaaat attgcccagg aggaaatcga
    27301 ccaagccatg caggccttgg ttgccaatca ggatttttat attgattcgt ctaatcaagt
    27361 ttactttttc gatgaagaaa ccaagaaaat tcgccaaaat ctacaggaac tggggcaatt
    27421 tgaattaaaa gatgggacct tgcaggctcg gaaatccttg gcctacagtt tagctcatct
    27481 ctttgaaggg cgagaccgtg tttctttttc acaagaattc cagaatctgg cccaggattt
    27541 gacgcatcca gaggactttc ctttgcaagc aactcaggtc aaggctgact tgcgagatta
    27601 tcaggaaaag ggaattggct ggttgcagat gctccatcat tatggttttg gtgggatttt
    27661 ggctgatgat atgggacttg ggaaaaccct tcagaccatt gcttttttga ctagtcaagt
    27721 gacaaaagaa agtcgagtct tgattctcgc tccgtcgggt ttgatttaca actgggcgga
    27781 tgagtttcag aaatttgccc cacagttgga tgtggctgtt gttcatggtt tgaaagcaag
    27841 tcgtgaggag attcttgccg agagccatca aatctatgtg actagctatg ccaccttccg
    27901 tcaggacagt gagttttatc aggggatggc ctttgacttc cttttcttag atgaggctca
    27961 ggtcatgaaa aatgcccaga ccaagattgc ccagaccttg agacaatttg tggtgccgtc
    28021 ggtctttgcc ttgtcaggaa ctccgattga aaaccatctg ggtgagttgt ggtctatttt
    28081 ccaaatcgtc atgcccggac ttttgccaag caagaaagaa tttatgaaat tgccagcaga
    28141 gcgagtggct cagtttatca agcctttcgt gatgcgacgc aagaaagaag aagttctgac
    28201 tgaattgcca gacttgattg aggtggttta taaaaatgaa ctggaagacc agcaaaaggc
    28261 tatttacctt gcccagttac aacagatgcg agaccatctg gctcaagtgt cagaacagga
    28321 atttcagcga agtcgtgtgg aaattttatc tggtttgatg cgcttgcgtc aaatatgtga
    28381 cactcctgcc ctgtttatgg aagattatca gggagccagc ggcaaactag atagtctccg
    28441 agacctactg gtacaggtgg cagacggcgg acaccgtgtc ttgattttct ctcagttcaa
    28501 gggaatgttg gaaaaaattg aacaagaact gccagacttg ggcctgactt cctttaaaat
    28561 tacgggttca accccagcca aggaaagaca agacatgacc aaggccttta atcaaggaga
    28621 aagagatgcc tttctgattt ccctcaaggc tggtggggtc ggtctgaacc tgacaggtgc
    28681 agatacagtg atcttggttg acctttggtg gaatcctgcg gtggaagcgc aagccattgg
    28741 ccgtgcccat cggatgggtc aggaagaaac ggttgaggtc tatcgcttgg tgaccaaggg
    28801 aaccattgaa gaaaaaattc aggaactcca agaacaaaag aaacatctgg tgtcacaagt
    28861 attggacggc acagagtcac gtggtagtct gaccctagca gaaattagag aaattttggg
    28921 aatttctgaa gccagcactt gaaaaatcaa gacaatacgc tataatgaag tgttgaaagg
    28981 aaatagaaaa tgaaacaaga acgatttcca ttggtgtcag atgacgaggt catgttgact
    29041 gaaatgccag tcatgaatct ctatgatgag tctgatctga tcagtaatat caagggtgag
    29101 tatcgagata aaaattattt agaatgggct cctattgctg aagaaaaacc agtaaaaccg
    29161 attgaaaagc aagtagggaa acctaaaaaa gctcctttag gggttaaaaa agaaggaaag
    29221 agctatgcgg aggtggcgcg tgaagaagcg cgtgcagact tgaaaaagaa acgctctgct
    29281 aactatctaa ctcaggattt cagccttgcg agacgtcatt ctcagcccag tctagttaga
    29341 cagggcaatc aaccgacaac tcctttccaa aaggaaaatc ctggtgaatt tgtcaaatat
    29401 agccaaaaat tgacccagtc tcattatatc ttggcggaag aagttcattc tatccctacc
    29461 aagaatgaag aagtgtcagc acctgctcca aagaaaaaca attatgattt tctaaagaag
    29521 agccaaatct acaataaaaa aagtaaacaa acagaacaag aacgtcgggt tgcccaagag
    29581 ttgaatctga ccagaatgac agaataggga gaaaacatgt caaagacata tcattttatc
    29641 ggaattaagg gatcagggat gagtgccttg gccttgatgt tgcaccagat ggggcacaag
    29701 gttcagggat cagatgttga aaagtactac tttacccaac gcggtcttga gcaggcagga
    29761 attaccattc ttccttttga tgaaaagaat ctagacggtg atatggaaat tatcgctgga
    29821 aatgcctttc gtccagataa caacgtcgaa attgcctatg cggaccaaaa tggtatcagc
    29881 tacaaacgtt accatgagtt tctaggtagc tttatgcgtg actttgttag catgggagta
    29941 gcaggagcac atggaaaaac ttcaacgaca ggtatgttgt ctcatgtctt gtctcacatt
    30001 acagatacca gcttcttgat tggagatggg acaggtcgtg gttcggccaa tgccaaatat
    30061 tttgtctttg aatctgacga atatgagcgt cacttcatgc cttaccaccc agaatactct
    30121 attatcacca acattgactt tgaccatcca gattatttca caagtctcga ggatgttttc
    30181 aatgccttta acgactatgc caaacaaatc accaagggtc tttttgtcta tggtgaagat
    30241 gctgaattgc gtaagattac gtctgatgca ccaatttatt attatggttt tgaagctgaa
    30301 ggcaatgact ttgtagctag tgatcttctt cgttcaacaa ctggttcaac cttcaccgtt
    30361 catttccgtg gacaaaactt ggggcaattc cacattccaa cctttggtcg tcacaatatc
    30421 atgaatgcga cagccgttat tggtcttctt tacacagcag gatttgattt gaacttggtg
    30481 cgtgagcact tgaaaacatt ttccggtgtt aaacgtcgtt tcactgagaa aattgtcaat
    30541 gatacagtga ttatcgatga ctttgcccat catccaacag aaattattgc gaccttggat
    30601 gcggctcgtc agaaataccc aagcaaggaa attgtagcag tctttcaacc gcataccttt
    30661 acaagaacca ttgccttgtt ggacgacttt gcccatgctt taaaccaagc agatgctgtt
    30721 tatctagcgc aaatttatgg ctcggctcgt gaagtagatc atggtgacgt taaggtagaa
    30781 gacctagcca acaaaatcaa caaaaaacac caagtgatta ctgttgaaaa tgtttctccg
    30841 ctcctagacc atgacaatgc tgtttacgtc tttatgggag caggagacat ccaaacctat
    30901 gaatactcat ttgagcgtct cttgtctaac ttgacaagca atgttcaata ggatgttccc
    30961 atggaaattc caattaagat cattcaggca agcaagtctg atttgcctga gataggggca
    31021 cttcagacct cgtcttttcc agctgaaaag cagcaacttt cccatatttt agaagaaagt
    31081 atccgtaagt gtgcggatac ctttcttcta gctagggatg aaaatcaact tttaggctat
    31141 attttatcaa gtccccagtc agacaatccg caatatctaa aagtacattc tttagtcatc
    31201 gagtctgacc atcagagaca gggcttggga acacttcttc ttgcagcctt gaaagaggtg
    31261 gcagttgatc tggattacaa agggattcgt ttggagagtc ctgatgagct gctttcctat
    31321 tttgaaatga acggttttgt tgatgaagaa gcaactttgc tctatgcaac tagccagggc
    31381 tatagtatga tttggtttaa tcccttttat ctggaggaac aatgaaaatc agacaagcaa
    31441 gattagaaga tttggatcgg attgttgaac ttgaatttga aaatttttcg gtcgaagaag
    31501 ccattcctcc ttctgtcttt gaagcacatt tgagagaaat tcagacctct tttctggttg
    31561 ctgaaaaaga aggaagaatc atgggttata tcgaaggacc agttggcctg caccgccatc
    31621 tgcaagacca gtcttttaca gaagaaataa aagactatag tcatgagcct ggtggttata
    31681 tctctgtgac ctgtctgtct attgccaagg aggcacaggg actcggactg ggtcagaaat
    31741 tgctgacagc tttgaaagaa gttgctcttg aagatgagag agatggcatt aatctaacct
    31801 gtcatgacta tctcatcgcc tattatgaaa aacatggatt tgtcaacgaa ggccagtccc
    31861 agtcaacctt tgcaggggaa acatggtatg atatggtctg ggaaatgaaa aaataagtta
    31921 ggaaaagtat catagattgc tttttttgga cttttaagat ataatattat ggattatcaa
    31981 caaggaggaa aaacttttga gtgaaaagtc aagagaagaa gagaaattaa gctttaaaga
    32041 gcagattctg agagatttag aaaaagtaaa aggctatgat gaagttctga aagaagatga
    32101 ggcagtagtt cgcactcctg caaatgaacc ttcagctgaa gaactcatga ctgattcctt
    32161 gtcaacggta gaggagatta tgagaaaagc tcctaccgtg cctactcacc caagtcaagg
    32221 tgtaccagct tctccagcag atgagattca aagagaaact cctggtgttc caagtcatcc
    32281 aagtcaagat gtaccttctt ctccagcgga agaaagtgga tcaagaccag gtcctgttag
    32341 acctaagaaa cttgaaagag aatacaatga aaccccaaca agggtagctg tttcctatac
    32401 gacggcagag aaaaaagcag aacaagcagg tccagaaaca cctacgcctg ctacagaaac
    32461 agtggatatc atcagcgata catcacgtcg tagccgtaga gaaggagcaa aacccgttaa
    32521 tcctaagaaa gagaagaagt cacatgtgaa agcttttgtg atttcattcc ttgtattcct
    32581 tgccttgctc tcagcaggtg gttactttgg ttaccagtac gtgctagatt ctttgttacc
    32641 tatcgatgct aattctaaga aatatgtgac ggttggaatt ccagaaggtt caaacgttca
    32701 agaaatcggt acgacgcttg aaaaagctgg tttggtaaag catggtctga tttttagttt
    32761 ttatgccaag tataaaaatt ataccgactt gaaagcaggt tactacaatt tgcaaaagag
    32821 tatgagtaca gaagacttac tcaaagagtt gcaaaaaggt ggaacagatg aaccgcaaga
    32881 acctgtactt gcgactttga caattccaga aggttatacc ttggatcaga ttgctcaagc
    32941 tgtgggtcaa ttgcaaggtg acttcaaaga gtctttgaca gcggaggctt tcttggctaa
    33001 agttcaagat gagacgttta tcagtcaagc agtagcgaaa tatcctactt tactggaaag
    33061 tttgcctgta aaagacagcg gtgcgcgtta tcgtttggaa ggataccttt tcccagctac
    33121 atactctatc aaggaaagca caactattga gagcttgatt gatgagatgt tagctgctat
    33181 ggataagaac ctatctcctt actatagtac tatcaaatct aaaaatttga ctgtcaatga
    33241 gttgttgacc attgcttcct tggtcgaaaa agaaggtgcc aagacagaag atcgtaagct
    33301 cattgcaggt gtattctaca atcgtttaaa tcgtgatatg ccacttcaaa gtaatattgc
    33361 aatcttgtat gcccaaggaa aactggggca aaatatcagt ctagctgagg atgttgcgat
    33421 tgataccaac attgattcac cttataatgt ttataaaaat gtaggtctca tgcctggtcc
    33481 agtcgatagt ccaagtctgg atgcgattga gtcaagcatc aatcaaacta agagcgataa
    33541 cctctacttt gtagcagatg tcacagaagg caaggtctac tatgctaaca atcaagaaga
    33601 ccacgaccgc aatgtcgctg aacatgtcaa cagcaaatta aactaaacaa actaaaatta
    33661 tgtgatacgt cacataattt tctttagaaa atattatcag aagaaagttg agaaaaatgg
    33721 cagaaaaaac atatcctatg acccttgagg aaaaggaaaa acttgaaaaa gaattagaag
    33781 aattgaaatt ggttcgtcga ccagaagtgg tagaacgcat taagattgcc cgttcatacg
    33841 gtgacctttc agaaaacagt gagtacgaag cagctaagga tgaacaagcc tttgtcgaag
    33901 gacaaatctc tagcttagaa acaaaaatcc gctatgctga aatcgtcaat agcgacgcag
    33961 ttgcccagga cgaagtagcg attggtaaaa cagtcaccat ccaagaaatt ggtgaggacg
    34021 aagaagaagt ttatattatc gtaggttcag ctggtgcgga tgcctttgca ggtaaggttt
    34081 caaatgaaag cccaattggg caggccttga ttggcaagaa aacaggtgac acagcaacca
    34141 ttgaaacgcc tgttggtagc tatgatgtaa aaatcttgaa ggttgaaaaa acagcctaaa
    34201 aacagaaaaa ggagtgggga ggcgatgtgc ttcactcact cctttttcca ttttgctatt
    34261 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttgcc gtatgtatgg ttactgactt
    34321 tgtcagtttc atctacaacc tcaaaac
//
LOCUS       DAVBCB010000018        33324 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVBCB010000018 DAVBCB010000000
VERSION     DAVBCB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.018"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..327
                     /locus_tag="VQU28_000840"
     CDS             <1..327
                     /locus_tag="VQU28_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2476218.1"
                     /translation="KPQTEKPEEETPREEKPQSEKPESPKPTEEPEEESPEESEEPQV
                     ETEKVEEKMREAEDLLGKIQDPIIKSNAKETLTGLKNNLLFGTQDNNTIMAEAEKLLA
                     LLKESK"
     gene            749..979
                     /locus_tag="VQU28_000841"
     CDS             749..979
                     /locus_tag="VQU28_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HET2476219.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAIKC"
     gene            1383..2096
                     /locus_tag="VQU28_000842"
     CDS             1383..2096
                     /locus_tag="VQU28_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HET2476220.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2154..2756
                     /locus_tag="VQU28_000843"
     CDS             2154..2756
                     /locus_tag="VQU28_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476221.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRFFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFERGSKMLNDLKSNYKK"
     gene            2892..3545
                     /locus_tag="VQU28_000844"
     CDS             2892..3545
                     /locus_tag="VQU28_000844"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HET2476222.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3555..4019
                     /locus_tag="VQU28_000845"
     CDS             3555..4019
                     /locus_tag="VQU28_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HET2476223.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAAREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4032..5300
                     /locus_tag="VQU28_000846"
     CDS             4032..5300
                     /locus_tag="VQU28_000846"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HET2476224.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5314..5574
                     /locus_tag="VQU28_000847"
     CDS             5314..5574
                     /locus_tag="VQU28_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476225.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5585..6946
                     /gene="pdrM"
                     /locus_tag="VQU28_000848"
     CDS             5585..6946
                     /gene="pdrM"
                     /locus_tag="VQU28_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HET2476226.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDKIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7100..8068
                     /locus_tag="VQU28_000849"
     CDS             7100..8068
                     /locus_tag="VQU28_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HET2476227.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8084..9076
                     /locus_tag="VQU28_000850"
     CDS             8084..9076
                     /locus_tag="VQU28_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HET2476228.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYISEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9294..10337
                     /locus_tag="VQU28_000851"
     CDS             9294..10337
                     /locus_tag="VQU28_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HET2476229.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     KVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10401..12086
                     /gene="lpdA"
                     /locus_tag="VQU28_000852"
     CDS             10401..12086
                     /gene="lpdA"
                     /locus_tag="VQU28_000852"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HET2476230.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEIIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMQSPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEIIGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            12150..13139
                     /locus_tag="VQU28_000853"
     CDS             12150..13139
                     /locus_tag="VQU28_000853"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HET2476231.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQU28_000854"
     CDS             complement(13206..14276)
                     /gene="xerS"
                     /locus_tag="VQU28_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HET2476232.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
     gene            complement(14860..16410)
                     /locus_tag="VQU28_000855"
     CDS             complement(14860..16410)
                     /locus_tag="VQU28_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HET2476233.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFIIIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGSLVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16426..17205)
                     /locus_tag="VQU28_000856"
     CDS             complement(16426..17205)
                     /locus_tag="VQU28_000856"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HET2476234.1"
                     /translation="MATIKEIKELLVTVKELESPIFLDLEKDNRSGVQKEISKHKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQVVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQU28_000857"
     CDS             complement(17192..18043)
                     /gene="ylqF"
                     /locus_tag="VQU28_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HET2476235.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EGLDGND"
     gene            complement(18250..18426)
                     /locus_tag="VQU28_000858"
     CDS             complement(18250..18426)
                     /locus_tag="VQU28_000858"
                     /inference="COORDINATES: protein motif:HMM:NF012948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2476236.1"
                     /translation="MQAGIPTDNEVSYALIYQQETLPATGSSTSALTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQU28_000859"
     CDS             complement(18478..24369)
                     /gene="iga"
                     /locus_tag="VQU28_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HET2476237.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGMGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGSIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSENTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEKKLEL
                     RNVSDIELYSQTNGTYRQHVSLDGIPENTDTYFVKVKSSAFKDVYIPVASITEEKRNG
                     QSVYKITAKAEKLQQELENKYVDNFTFYLDKKAKEENTNFTSFSNLVKAINQNPSGTY
                     HLAASLNANEVELGPDERSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEK
                     LSLKNVAISGKNDIGSLANEATNGTKIKQVHVDGVLAGERGVGGLLAKADQSSIAESS
                     FKGRIVNTYETTDAYNIGGLVGHLTGKNASIAKSKATVTISSNTNRSDQTVGGLAGLV
                     DQDAHIQNSYAEGDINNVKHFGKVAGVAGYLWDRTSGEEKHAGELTNVLSDVNVTNGN
                     AITGYHYTGMKVANTFSSKANRVFNVTLEKDEVVSKESFEERGTMLDASQIVSKKAEI
                     NPLTLPTVEPLSTSGKKDSDFSKIAHYQANRALVYKNIEKLLPFYNKSTIVKYGNLVK
                     ENSLLYQKELLSAVMMKDDQVITDIVSNKQTANKLLLHYNDHSSEKFDLKYQTDFANL
                     AEYNLGNTGLLYTPNQFLYDRDSIVKEVLPELQKLDYQSDAIRKTLGISPEVKLTELY
                     LEDQFSKTKQNLGDSLKKLLSADAGLASDNSVTRGYLVDKIKNNKEALLLGLTYLERW
                     YNFNYGQVNVKDLVMYHPDFFGKGNTSPLDTLIELGKSGFNNLLAKNNVDTYGISLAS
                     QHGTTDLFSTLEHYRKVFLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYS
                     IGVYDRITSATWKYRNMVLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALN
                     DFVEENARETAKRQRDHYDYWYRILDEQSREKLYRTILLYDAYKFGDDTTSGKATAEA
                     KFDSSNPAMKNFFGPVGNKVVHNQHGAYATGDGVYYMSYRMLDKDGAITYTHEMTHDS
                     DQDIYLGGYGRRNGLGPEFFAKGLLQAPDQPSDATITINSILKHSKSDSTEGSRLQVL
                     DPTERFQNAADLQNYVHNMFDLIYMMEYLEGQSIVNKLSVYQKMAALRKIENKYVKDP
                     ADGNEVYATNVVKELTEAEARNLNSFESLIDHNILSAREYQSGDYERNGYYTIKLFAP
                     IYSALSSEKGTPGDLMGRRIAYELLAAKGFKDGMVPYISNQYEEDAKQQGQTINLYGK
                     ERGLVTDELVLKKVFDGKYKTWAEFKTAMYQERVDQFGNLKQVTFKDPTKPWPSYGTK
                     TINNVDELQALMDQAVLKDAEGPRWSNYDPEIDSAVHKLKRAIFKAYLDQTNDFRSSI
                     FENKK"
     gene            complement(24578..25756)
                     /locus_tag="VQU28_000860"
     CDS             complement(24578..25756)
                     /locus_tag="VQU28_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476238.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLIFNGIGRSNC
                     VCRNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLPLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWILNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQU28_000861"
     CDS             complement(25926..29576)
                     /gene="addA"
                     /locus_tag="VQU28_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HET2476239.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEICSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FAKLGQLSDQITILDYQERYHQDTWKLAKTFQTFMSHFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNKHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALVEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIDEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQIIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQU28_000862"
     CDS             complement(29573..32848)
                     /gene="rexB"
                     /locus_tag="VQU28_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HET2476240.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRADLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQTTEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPISPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33048..33203)
                     /locus_tag="VQU28_000863"
                     /pseudo
     CDS             complement(33048..33203)
                     /locus_tag="VQU28_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
BASE COUNT         9515 a         6539 c         6861 g        10409 t
ORIGIN      
        1 aaacctcaga cagaaaaacc tgaggaagaa acccctcgag aagagaaacc gcaaagcgag
       61 aaaccagagt ctccaaaacc aacagaggaa ccagaagaag aatcaccaga ggaatcagaa
      121 gaacctcagg tcgagactga aaaggttgaa gaaaaaatga gagaggctga agatttactt
      181 ggaaaaatcc aggatccaat tatcaagtcc aatgccaaag agactctcac aggattaaaa
      241 aataatttac tatttggcac ccaggacaac aatactatta tggcagaagc tgaaaaacta
      301 ttggctttat taaaggagag taagtaaagg tagcagcatt ttctaactcc taaaaacagg
      361 ataggagaac gggaaaacga aaaatgagag cagaatgtga gttctagttc tcattttttt
      421 catgaaaatg tgcaaaatat agtagattga aactagaata gtatacctct acttctaaaa
      481 cattgttaga aatcgatttg actgtcctgt tcttatttca ttttactata tcttaacaga
      541 tagtgtaaat aaagataaac tatttactgg ctaattaatc agttaaacac tagttaagga
      601 gtaatgatga aaaaaagaac aatactatta ttgatggcca gtctgttagc tcttgtctta
      661 ggagcatgtg gtttcttgga catattgatc ctggatcatt ctcatcagga ttactcttta
      721 ctgctatttt agaaactggg gtggtttgat ggaaagtatt ggtattggtc ttgttatcgt
      781 ttcacattcc aaacacattg cagaaggtgt tgttgaactg attagtaaag tagctaaaga
      841 tgttccgatt acttatgtag gaggaaccga gggcggagga attggaacga gttttgatca
      901 agtagatagg gttgtttccg aaaatccagc agatacttta cttgcctttt ttgacctagg
      961 ttctgctata aaatgttaag taaatgattt caaaatcaga aagggattgc tttgggcagt
     1021 tcctttttaa tgtaaatgta aaaggagtag aatcatagtg tttctaaatt gtgaatcaat
     1081 caaaactgat tgtgatgggg ctattctagc tttagaatcc ttcaaaaatt aaaatttaag
     1141 gcaatcaatt acttggaaga gtatgaaagc atttagttta taggaattct aggcctagaa
     1201 ttacatatgt atttatgaag ataggggtgt tcgatagtta gtattgttct gttctgaaag
     1261 atttgagctg tcagttgtat agaaagtgtt cgaatttttt ttaagtgatt aaattagtta
     1321 attgtatgag gttctttatg atataatgtt cttaatgaat tttcagaaag gaaaacctca
     1381 aattgttcta caaatttcta ctcttcgacc tcgaccacac tcttcttgat tttgatgctg
     1441 ctgaggatgt ggctttgacc caacttctaa aagaagaagg agttgcggat attcaggctt
     1501 ataaagatta ttacgttcct atgaacaagg ctctctggaa agacttggag ctgaagaaaa
     1561 tcagtaaaca agagctggtt aacacgcgct tttctcgttt atttgctcat tttggacagg
     1621 aaaaagacgg tagttttctt gcccagcgtt accaatttta cctcgcccag cagggacaaa
     1681 cactatcggg cgctcatgat ctcttggaca gcctcattga gcgtgattat aacttgtatg
     1741 ctgcgacaaa tggcattact gccattcaga caggacgttt ggctcaatct ggtctagcac
     1801 cttatttcaa tcaagtcttt atctcagaac agttgcaaac tcaaaagccg gatgctctct
     1861 tttatgaaaa gattggccag caaattgctg gatttagtaa agaaaagacg ctgatgattg
     1921 gagattctct aaccgccgac attcaaggtg gcaataatgc ggggattgat actatctggt
     1981 ataatcctca tcacctcgaa aatcacacac aagcccagcc gacttacgaa gtctattctt
     2041 accaagactt gctggattgt ttagataaaa atattcttga aaagatcaca ttttaaagga
     2101 gacgagctaa tgactacaaa aaagctaata ttactattga agagtacatt gaaatgtctg
     2161 aagttgattt taatgaagct gttaattatg aatttacatc tgacacttgt caattagcaa
     2221 atagtattta tcaatctctt tttaagtttt ttgataagaa aaatttctct ggcgatttaa
     2281 tttttacttg gaaatctcca tcattagtca aagaagggga ttatattggg agaagggatt
     2341 cacaagtaga taatcttaga gtaataggaa atatatttcc gaattatctt actaatcgaa
     2401 aatatagcct caatatgaat cgtaatggct gtatgggaga ttttcctcat gacttttttg
     2461 atatatacct agatcatgta gcaaaatatg cctacgaaca aaaagttaat aatattaaag
     2521 agtattatcc tttaaaaaga gcgattttac accaagagaa tgcattgtat tttcgatttt
     2581 tttctaattt tgacgacttt ttagaaaaaa attatttaaa gactatatgg caagtttcta
     2641 aagaaactcc tttttctgaa atggatttta atatgtttaa aaatatttca gaaaagataa
     2701 tcttcgaaag aggtagtaaa atgcttaatg atttaaaaag taattataaa aaatagccag
     2761 tctttataaa ctgtattcac gtattcaaat cgtatatagt cgatttatta aatctttaga
     2821 taaactgtaa aaagtggttt ccatagccac tttttgctat aatagaggca gtaaaaatga
     2881 aggagtcggc tatggaacac tcgtcttggc atgctttgat taaggcgcaa ttacctgagg
     2941 gttatttcgg gaaaatcaat cagtttatgg agcaggtcta ttctcagggg attatttatc
     3001 cacccaagga aaaggttttt caggctctct tgacaacact gcttgaagaa gttaaggtgg
     3061 taattctagg gcaagacccc tatcacggac caggtcaagc gcagggcttg agtttttctg
     3121 tacctgactc tatcccagct ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg
     3181 atatcggagt taagaaatct catgatttga cagcttgggc tgagcaagga gtcttgcttc
     3241 ttaatgcttg tttgactgtt cctgctggac aggccaatgg tcatgctggt caaatctggg
     3301 agccttttac tgatgctgtg attcaggtgg tcaatcatct agatagacca gtcgtttttg
     3361 tactctgggg agcttatgca cgtaagaaga aggccttagt taccaatcct catcacttga
     3421 ttatcgaatc agcccatcca agtcctttgt cggtttatag aggattttgg ggttccaagc
     3481 ccttttccaa ggccaatgca ttcttaaaag agacaggaca agagccaatc gattggctta
     3541 gataaggaga aaatatgcct cagttagcga cgatttgcta cattgataat gggaaagaac
     3601 tgctcatgct ccatcgtaat aagaaaccca atgatgtcca tgaagggaaa tggattggtg
     3661 tgggtggtaa gctagagaga ggagagacgc cccaggaatg cgcggcgcgt gaaatccttg
     3721 aagaaacagg gctcaaagcc aagccagttc taaaaggtgt catcactttt cctgaattta
     3781 caccagattt agactggtac acctatgttt ttaaggtgac ggagtttgag ggcgacttga
     3841 ttgactgcaa tgaggggacg ctagaatggg ttccctatga cgaggttttg agcaagccga
     3901 cttgggaagg tgaccacacc tttgttgagt ggcttttaga ggataaaccc ttcttttcag
     3961 ccaagtttgt ttatgatggg gataaattgt tggataccca agttgatttc tatgaataaa
     4021 ggagaaagca gatgctacta atcaaaaatg gtcgtgtaat ggatcctaag tctggtttgg
     4081 atcaagtttg tgatgtctta gttcaagatg ggaaaattat caaaattgcg cctgagatca
     4141 cggaagaagg agcagaaacg attgatgcta ctggtcttgt ggttgctcct ggtttggtcg
     4201 atattcatgt gcatttccgt gaacctggtc aaacacataa agaagacatt catactggtg
     4261 ccctggcagc cgctgcaggt ggttttacta ctgtcgtcat gatggctaat accagtccaa
     4321 ccatttcaga cgtggagact ttgcaagcag ttctccagtc agctgccaaa gagaagatta
     4381 atgtcaagac agttgcgacc attactaaaa actttaatgg taaaaacttg actgacttta
     4441 aggcgctctt agaagctggt gcggttggtt tctctgatga cggtattccg cttgagagca
     4501 gtaagattgt caaggaagcc atggaggaag ccaaaaaact caataccttt atcagccttc
     4561 atgaggaaga tccaggtttg aacggtgttc ttggctttaa tgaaaatatt gctagagaac
     4621 atttccatat ctgcggtgct actggggtgg ctgagtacgc tatgatggcg cgtgatgtca
     4681 tgattgccta tgcaactaaa gcccatgttc acatccagca tttgtctaag gaagaaagtg
     4741 ttaaagtagt ggagtttgct caggggttag gtgcagaagt cacagcagaa gtagcgccac
     4801 agcatttctc taagacagaa gcacttcttt taacacaagg tagcaatgct aagatgaatc
     4861 caccgcttcg tttggaatca gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca
     4921 tcacagttat tgcgactgac cacgcgcctc atcatgtaga tgaaaaaaat gttgaggata
     4981 ttaccaaagc gccatctggt atgactggct tagaaacatc cctgtctctc ggcttgacct
     5041 atttagtaga agctggtgag ttgagcttga tggaattact tgaaaaaatg acatacaacc
     5101 cagccaagct ttacaacttt gaagcaggtt acttggctga gaatggtcca gcagatatca
     5161 ctatttttga tgccaaggct gaccgccttg tggactccca ttttgcttcc aaagcagcta
     5221 attcaccatt catcggtgaa accttaaaag ggcaggttaa atataccatc tgtaagggac
     5281 aaatcgtcta tcaagcttga taggagttag aatatgaatt attttcgaaa acgtagggag
     5341 agacaagcga aatctaatag tggaatttac tgtcccgcag ctaatcgttc caaaattcaa
     5401 tatgaaacta aagaaaaagc tgaccatgcg gttcaatatg ataacggagg tttagttaga
     5461 agctattatt gtcgtacatg tgcgtgttgg catactactt caaaatctaa taaacctccg
     5521 ctaagtttga aaaactatgg tctgaaactc tttggtttag ataaatcaga atagaaagag
     5581 aagtatgtat aagacaaagt gtttacgaga gaagttagta ttatttttaa aaattttctt
     5641 cccaatcctg atctaccaat ttgccaatta ttctgcctct tttgttgata ctgcaatgac
     5701 aggtcaatac aacactatgg acttggctgg tgtatctatg gcaaccagta tctggaatcc
     5761 tttctttaca tttctaacag ggattgtgtc agccttggtg cctatcattg gtcaccatct
     5821 tggtcgaggc aaaaaggaag aagttgcgtc tgatttttac caatttattt atttggcctt
     5881 gggcctatct gtggtcttgc tggggatggt acttttcttg gcaccaacaa tcttgaatca
     5941 tattgggtta gaagcagcag tagcggcagt agcggttcgc tatctttggt ttttatctat
     6001 cgggattatc cccttgttgc tctttagcgt cattcgttcc ttgctggatt cgctgggctt
     6061 gaccaaactg tccatgtacc tcatgctttt gttactccct ctcaatagcg gatttaacta
     6121 tctcttgatt tacggtgcct ttggtgttcc agaactggga ggggctggtg ctggtttagg
     6181 aacatccttg gcctactggg tcttgcttgg gatttctgtt ctggttttat ttaaacagga
     6241 gaagctcaaa gccttacacc ttgagaaacg aattccactt aatatggata aaattaagga
     6301 aggagttcgt ttaggtctgc ctattggggg aactgtcttc gcggaagtgg ctatcttttc
     6361 agtggttggc ttgattatgg ctaagttttc gtccttgatt atagctagtc accagtcagc
     6421 tatgaacttt tcaagtctta tgtacgcctt tcctatgagt atctcatcgg ctatggctat
     6481 tgtcgtttcc tatgaagtgg gagccaagcg atttgatgat gcgaaaacct atattggtct
     6541 aggaagatgg actgccctca tttttgcggc cttcacctta accttccttt acatttttag
     6601 gggaaatgtg gccagtcttt atggtaacga cccaaaattt atcgatttga cagcgcgttt
     6661 tttaacttat agtcttttct tccagttagc agataccttt gcggcgccgc ttcagggaat
     6721 tttgcggggg tataaggata cagttattcc tttttacctt ggtttgcttg gttattgggg
     6781 cgtagcaatc cctgtggcta cgctatttga ttccctaaca gattttggag cttattctta
     6841 ctggatcggc ttgattatta gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac
     6901 tgtgattatg aagagatttg aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa
     6961 aatattcttg ataagaaaat cccttgtttt cacggggttt tcttttttat tttgtgaaat
     7021 ttcttttaga aaaactttga cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga
     7081 aagttagaaa ggcaagaaga tgtcaacttt agataaaaat cttttgctag aaatgttccg
     7141 taagatggaa gaaatccgtc gcatggactt aaaaattgca caattagtaa agaaagggaa
     7201 agtgccagga atgacgcact tttctgttgg tgaagaggca gctaacgtgg gggctatgtt
     7261 agctctcaat ccagatgatc tgattacctc aaaccaccgt ggacacgggc aagctattgc
     7321 taaagggatc gacctcaacg gaatgatggc tgaaatcctt ggtaaataca ctggaacctg
     7381 taaagggaaa ggtggatcta tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa
     7441 tggtatcgta ggtggtggta tggggatcgc tgtcggtgca gccctcagtc agcaaatgca
     7501 aaataccggt aaaatcgttg tctgcttctt tggagatggt gcgaccaatg aaggtgtttt
     7561 ccacgaagca gtgaacatgg cttctatctg gaacctgcca gtcattttct attgcattaa
     7621 caacggttac ggtatctctg cggatatcaa gaaaatgacc aatatagaac atatccatca
     7681 acgtagcgcc gcttatggaa ttcctggaat gttcatcgaa gacggtaaca atgtcatcga
     7741 tgtctatgaa ggatttcaga aagctgtaga ccatgttcgc agtggcaatg gtccagtctt
     7801 gattgaaagt gtaacttatc gctggctcgg tcactcatca tctgaccctg gtaaatatcg
     7861 tacgcgtgaa gaagtggaat tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta
     7921 ccttattgaa aataacattg caagtgccga agaattggaa gaaatccaag cgcaagtaaa
     7981 ggaagcagta gaagcttctg ttaaatttgc agaggaaagc ccattcccac cgcttgaatc
     8041 agcctttgaa gatatttacg cagactaagg agaaagagat aaaatggaaa caaaaacaat
     8101 gtccttccgt gacaccatta tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt
     8161 gttcttgatg ggagaagacg tcggtgtctt cggaggagac ttcggtactt ctgttggaat
     8221 gcttgaagaa tttggtccag aacgcgtccg tgactgtccg atttctgaag ctgccatctc
     8281 tggagcagca gcaggagcag ccatgacagg acttcgtcca atcgtcgata tgaccttcat
     8341 ggacttctct gttattgcca tggacaatat cgtcaaccaa gctgctaaaa cacgttacat
     8401 gtttggtggt aaaggtcagg ttccaatgac tgttcgttgt gcagctggta acggagttgg
     8461 ctctgccgcc cagcactcac aatctctaga gtcttggttt actcacattc caggacttaa
     8521 ggttgtggca cctggaacac ctgcggacat gaaaggcctg cttaagtctt ctatccgtga
     8581 taaaaaccca gttattatac ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc
     8641 agttgatcca gactacacaa ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga
     8701 tgtaacagtt gttacttatg gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt
     8761 agcagaagaa ggaatttcag ttgaaattgt tgacccacgt acccttgttc cgcttgataa
     8821 ggatatcatc ataaactcag tgaagaagac tggtaaggtt gttctggtca acgatgccca
     8881 caaaacaagt ggctatatca gtgaaatttc agctattatt tcagaatcag aagcatttga
     8941 ctatctagat gcaccaatcc gccgttgtgc aggagaagat gtgccgatgc cttatgcaca
     9001 aaacctagaa aatgcaatga ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac
     9061 ttataacaaa gaatagagtt acataagata agttatgtaa ataaaataaa gctgacgaga
     9121 aactttgtat cttttaggtg cagagctctt cgtcattaat atcttatatt cgagcacggg
     9181 ctaaaagctc ggaaaaaaga taaatctcct tggcttcatc gaagccatcg tcgatttcct
     9241 atttttcagt cgcttttgac gccctttgta tcataaatag aattggagag gtcatggctg
     9301 atgacaagct aagagcgact cctgcagcta gaaagttagc ggatgattta gggataaacc
     9361 tctacgacgt ttctggctca ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt
     9421 ataaagacac aaacgtggtt cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata
     9481 acattgcttg gcaggaaatc caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg
     9541 ttttggccct gcttcctgaa aatatcgaaa acgatagcat caaatctcct gctcagattg
     9601 aaaaagtgga agaagtccct gataacgtaa caccatatgg tgaaatcgag cgtattccaa
     9661 tgacaccaat gcgtaaggtt attgcccaac gcatggttga atcttacttg actgcgccaa
     9721 ccttcaccct caactatgaa gttgatatga ctgaaatgtt ggctcttcgt aagaaggttc
     9781 ttgagccaat catggaagca actgggaaga agactactgt aacagacctt ctttcgcttg
     9841 cagttgttaa gactcttatg aaacacccat acatcaacgc ttcattgaca gaagatggca
     9901 agactattat cactcacaac tatgtcaatc ttgccatggc agttgggatg gataatggat
     9961 tgatgacacc tgttgtttac aatgctgaga agatgagtct ttcagaacta gttgtagcct
    10021 ttaaggatgt tattggccgt accttggatg gtaaattggc tccaagtgag ttacaaaatt
    10081 caacattcac aatcagtaat ttgggaatgt ttggtgttca gtcctttggc ccgattatta
    10141 accaacccaa ctcagctatc cttggtgtca gttcgacaat cgagaagcca gttgtcgtca
    10201 atggtgaaat tgtgattcgc ccaatcatga gtttaggatt aaccattgac caccgtgtcg
    10261 tagatggtat ggctggtgct aagtttatga aggacttgaa agagttgatt gaaaccccaa
    10321 tctcaatgtt gatttaagag caagagtatt catttttaag atatagataa aggaaggaag
    10381 acatggcctt agaaataatt atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg
    10441 tccaatggaa taaaaaagtc ggagaatttg taaaagaagg agaaatcctt ttggaaatca
    10501 tgactgataa agtcagcatg gaattggaag ccgaagaaga tgggtacttg attgccattc
    10561 tcaaaggaga tggtgaaact gtccctgtaa cggaaattat cggttacctt ggtgaagaaa
    10621 gggaaaacat cccaacagct ggagcagcat cgccagaagc tagccctgta cctgtagcaa
    10681 gtacctcaaa cgatgatggt aagagcgatg atgcttttga tatcgttgtg attggtggag
    10741 gtcctgctgg ttatgttgca gccattaaag ctgcccaact cggcggtaag gttgcccttg
    10801 ttgagaaatc tgaacttggt ggaacctgct taaaccgtgg atgtattcca accaagacct
    10861 accttcataa cgctgaaatt attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga
    10921 ttgaaaatcc taacttcact gttgatatgg aaaaactttt agaaactaaa tctaaagttg
    10981 ttaatactct tgttggtggt gttgcaggac ttcttcgtag ctacggagtt actgttcata
    11041 aaggaattgg tacaatcact aaagacaaga acgtcttggt aaatggttct gaattgcttg
    11101 aaaccaagaa aatcattctt gctggtggtt caaaagtcag caagatcaac gtccctggta
    11161 tgcaatctcc acttgtcatg actagtgatg acattcttga aatgaacgaa gtgccagaaa
    11221 gccttgttat catcggtggt ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat
    11281 ttggttcaaa agtgactgtt atcgaaatga tggaccgtat agttccagct atggatgcgg
    11341 aagtttctaa gaaccttcgc ttgatccttg agcgtaaagg aatgaccatc ttgactggta
    11401 ctaaactgca agaaatcatt gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag
    11461 acgatatcat cgcaagcaaa gctcttcttt caattggtcg tatgccagac cttgaaggta
    11521 ttggagaggt tgagtttgaa ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa
    11581 cttcagttcc aggcatttat gcaccaggtg acatcaacgg tactaagatg ttggctcacg
    11641 cagctttccg catgggtgaa gtttccgctg aaaatgccct taaaggaaat catgcagttg
    11701 ccaaattgaa tttgactcct gcagccatct acactctccc tgaagtagca gcagtaggtt
    11761 tgacagaaga acaagcccgt gagaaatacg atgttgccat cggtaagttt aactttgctg
    11821 ctaacggtcg tgctattgca tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata
    11881 agaaatacgg agaaatcatt ggtgtacaca ttattggtcc tgcagccgca gaattaataa
    11941 acgaagcatc aagcatcatc gaaatggaaa tcactgttga ggaaatgctg aagaccatcc
    12001 acggacaccc aacttactct gaagtgatgt atgaagcgtt tgcagatgtt ctaggaatgg
    12061 ccatccattc acctaagaaa aaataaaacg aagatagatt agactggaag gttatttcca
    12121 gtctctatca tataaagtaa agggatatca tgaaatacat tatcaatcat tcaaatgaca
    12181 ctgcttttaa tattgccttg gaagaatacg cctttaaaca ccttttggat gaggatcaaa
    12241 tcttcctact ttggattaac aagccatcta tcattgttgg tcgtcaccag aacactatcg
    12301 aagaaatcaa ccgtgattat gttcgcgaaa atggtattga ggtagttcgc cgtattagtg
    12361 gtggtggagc tgtttaccac gatttaaata acctcaacta cacgatcatc tccaaagaag
    12421 atgaaaataa ggcatttgac ttcaagagct tctcaactcc ggttatcaat accctggctc
    12481 aactcggggt taaagctgag ttcacaggtc gtaatgacct tgagattgat ggtaagaagt
    12541 tctgtggcaa tgcccaagcc tatatcaacg gccgtatcat gcaccacggt tgcttgctct
    12601 ttgacgttga tttgtcagtc ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat
    12661 caaaaggtgt gaaatccgtc cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa
    12721 aaatcacagt cgaaaaattc cgtgatttac tcttggaata catgaaaaaa gagtacccag
    12781 agatgactga atacgtcttt tcagaagaag aattggccga aatcaatcac atcaaggata
    12841 ctaagtttgg aacttgggac tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg
    12901 gaaccaaatt cactagtggt aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc
    12961 aagacatcaa gatttatggt gacttctttg gtattgaaga cgttgctgca gtagaagatg
    13021 tccttcgtgg ggtgaaatac gaacgcgaag atgttcttaa ggcgctaaaa accattgata
    13081 tcacacgcta cttcgctggt attagccgtg aagaaatcgc tgaagcggta gttggataaa
    13141 tcaaaaaaag aagttggcca acttcttttt ttgatattta tttacataat ttaaaatacg
    13201 tgaaatcata aactatctag agcattcttt tgttcatcat taacaatatg ggtatagagg
    13261 tcagtgactt gtgtgctggc atgtcctagt tggtgactga ctaaaacttg tgatttagtc
    13321 gcatcataga gcctagttgc tagcgtatgg cgcagtttat ggggtgttac acgtacttta
    13381 aaatcctctg agtatttagc aaccattttc tcaacgctag aagcatcgat acgattagga
    13441 acacctctgt agagagttaa aaaaagggct gtatctgttt tttccgtttt atagcgttga
    13501 ttccgaatgg ccagataatt ctctaaataa ggtttagcaa aagcagcgac attgactgag
    13561 tcacgtttgc caccttttcg agtaacatca ataaccatca tttttagatt gagatctctt
    13621 agatctagat taacagcttc agataagcga acaccagatg ccaagagaag ggcaataatg
    13681 gctaaatctc gttctttatt tttgttgaat gatgagagag ctcgatttga aagctgttgt
    13741 gggtgctctt gatcgatata agttagaaaa ccttctgttt catcacctag aaagagtttt
    13801 tgcttgatat tttcagctct ggcagcaagg gtttctttct ttttcttggt tgaaactttt
    13861 ttcattacat tacgatagaa ataaggttcc ccctgatcgt tttcaacctc ctcggttaga
    13921 tacttgtaaa gactagaaag tgctgataag gttcgattga tagttgtctg tgaaacacct
    13981 tgttttgttg tattagcatt cagcaaggga cgttcacgta gataaaggat aaaggattcc
    14041 atgtctttct tagacatatt ttccaaaact gataaaggaa tatcggatat tttatcagcg
    14101 tttgaaatac cagactccaa aacccagctg aaaaatcggt catattcctt aaggtattcg
    14161 tacagggttg taaaactgta gggcacagcc agcttagatt ggtagtattc cagaacatac
    14221 cagggcatga gttgttttag tttgtcgatt cgttccagta aaatctcacg tttcatgtat
    14281 tttctccata agtaagtata ctagtagtat agcatagact tgtatatttt tcaagaaaat
    14341 tatagttttt cagaaagatg ttataggagt ttttcaaagt gataaaaaga cttagaccta
    14401 caaacaaaac agaataagga aaataataaa aatctcaccc actaaacttc tgttaatact
    14461 acataactga agagctatat aaggagctat gagatacaaa acacattgta taatagattg
    14521 aaactagaat agtacatctg gacttctaaa acattgctag aaattgattt gattgttctg
    14581 attgttttat cctatttcta tttcatttca ctatataatt gctattgttt tctttttcat
    14641 gattttaatc ttactgtaca gatttgatac agtaaactcc tttctagctt tatactttat
    14701 catttctaac ttaattatag tcttattttt aataaagttc aattatttat atgtaaaatt
    14761 cctcatcagt aaaaaataac cgatctcatt tctgagaatc ggttttctaa ataaatcaag
    14821 ctgagtattt acataacata aattatgtaa atactcatac tacaacaaca aatctttgac
    14881 ttttccaatt tcactttttg gaataaccag gtgaatcata tcacccagat acattctggt
    14941 tgagccgtta actgtttggc tcttgccatt atggacttga gttgtgatga ggacgttgtg
    15001 tggtaagttg agttcatgaa cttgtttccc agcaatttta tcagaaactg gtatttcgat
    15061 aagtgtaact tctccttcgc tagatacttc ttctggaagc attttttcca gcatggcttc
    15121 atagactggc gtacctttga gcaaatccat gataatataa gaaacaagag tgacaagacc
    15181 aagtggcatg aggttgcgaa tatctcctac catctcagtt acgaggatca tagcggttaa
    15241 gggagctttt gatatggctc caaaatagcc actcattcct agaatgacaa atatagggaa
    15301 ttgctcttga ctgacaagtc caagattgac acagataaca ccaactaagg aaccaagcaa
    15361 agaaccaaga gctaaaatgg ggaggaaaat tcctcctggc agtccacttc catagctaat
    15421 catactccaa ataaagcgga tcaaaaagta agctaataaa acttggaaac taaaattttg
    15481 ttcagttaaa gaaaggacaa gctgatttcc gccaccaatg atttgaggta agaagattcc
    15541 gactggtatg ataatgataa aagccaagat gggataataa gccctatcca aatggatttt
    15601 ttgaccaatc aagtcataaa ctcttccaac gtttaataca gctttctcat agagaaaacc
    15661 tgaaaatcca aggaaaattc ccatgacgag atatatccaa tactggtcta gggtcatggg
    15721 aggaatgtta tctggcatat ccaatactgg tgtcaaaccg aacatgagta gagacacaaa
    15781 gtttgctacg atgctggctg ctagagttga gacccagaaa aagcgcgaaa aatggtgata
    15841 gacttcttct acaacaaaga gaagtgctgc aataggagca ttaaaggctg cggctaaacc
    15901 tgctgcagct ccactggcaa tcaaggaacg ttcctctact ggactggatt tgagccactt
    15961 ggcaattcct ttaccaccaa ctgctccaag ttgaatgctg ggtccctctc gacccagcat
    16021 gagtccactg gcaatagcaa gaatacctag cacatatttt ttccaaagaa tgccccacca
    16081 gttgagggac atgaggcctt tcagttcggc ttcgacttga ggaattcctg agcctttaat
    16141 atctttttct gaccgtgtta gtttggcact gagccaacag atgagtatat aaaacaaaac
    16201 cagtacaaaa agattgcgca ctaagtaccc ttgatcttga taaactcctt gtatcaggtg
    16261 gaagcccttt tcaattaaga aacgaaagga tccgacgata attccaacga tgaggccgac
    16321 aatgattcct cgaccaactt gggatagtat agtgctggat gcaaaggcga attctttctt
    16381 agaacggact atttccgact gttcctccat ggtatttcct ttcaattaac tttctttttt
    16441 acctaaaacc agtgatttaa cgggttcaaa gctggttcgg tgaattgggg taactcctag
    16501 ttttgtgagg ccttccagat gtttagctgt gccatatcct gcattagtag cgaaatcata
    16561 gccagggaac tgctgatcgt attccttcat caattcatca cgtgttacct tggctactat
    16621 agatgctgct gcgatagaga gggagttggc atctcctttg ataatggagg tttgtgaaat
    16681 gggcaagtcc agtttcatgg catcaatcaa aaggtgctct ggttgagggc tgagctggga
    16741 gattgcttct tgcatggcta gtttggttgc ttcatagata ttgacttggt cgatgacctg
    16801 attatctatg ataccaattc caatcgacaa ggcttggtct tgaacgactt ggaaaatctc
    16861 cagatgtttc tttttaggaa ttttcttgct gtcgttgaga cctttaatct tacaattttt
    16921 aggtaaaata acggccgcag cgactacagg accagcaaga ggaccacgac caacctcatc
    16981 aatacctgca attaaggtca atccttgctt ataaagttct ttttcataag aaagcatgga
    17041 ttccaagcgc aaattttcat ctaattcagc ttgaatggct cttttatgct tgctgatttc
    17101 cttttgaact cctgagcgat tatccttttc aagatctaaa aaaatagggc tttctaactc
    17161 cttgactgtc acaaggagtt ctttgatttc tttaatcgtt gccatcgagg ccttccaatg
    17221 tatctaaggt atagttaccg agtttgccat cacggacttc cttcacgaag agactgtaaa
    17281 aacggtcata gtcatcacgg aaaccgaggg cgcgggtcat atccataata atcacaggcg
    17341 cttcttcttc aattttcatt tgtttgaagc gttcagccag cttttctgga taatgttctt
    17401 tgaaataatt gataccaaaa atggtaacct catccatagg aagcaactga tccttgatag
    17461 ctccagtcaa tgccaactta agtgcaacag tttcatcctc aaacttaggc cagagaatcc
    17521 ccggtgtatc caagatttcc agatctttat tggttttaag ccattgttga ccttttgtga
    17581 cccctggctt gtttccaaca acagcaatct ttttaccagc caaacggttc atcagagttg
    17641 atttaccagc gtttggaatc ccaataatca tggtacgcaa ggtttcaatc tgaatcccac
    17701 gttctttctg gcgagcaatc ttatcagcca tgagcttctt ggccgcatct gttacaactt
    17761 ttacagtcac ttgctctttg gagttgatag ctagcgtctg gattccttgt gattcaaaat
    17821 actgacgcca ttccttggtc attgctggat cagccaagtc ggccttgttt aaaatcaaga
    17881 gttttggttt atcaccaaca atcttggtta acataggatt ttgactagat agaggtaagc
    17941 gtgcatcaac taaaatcgtc acaaaatcaa caaattttaa attctcctgc acctgtcgac
    18001 gagctttaga catgtgacca ggaaaccatt gaatagtagc cattgagttt tttcctttca
    18061 tagcaaggat ttagatcccc tattttattt tactattgtt tgaacataga gagttttcaa
    18121 atgaatgatg tccaaacagt tcttgtttta tgacatagaa atctacttta tattatatca
    18181 tgccttcttt attttgtccg atgatagcct tttaccctat atagaaaaaa gccatcaaat
    18241 gatgactttt cagagggagc aaaaaactgt tcccgtcata ttatgaacca aaagaactaa
    18301 actaccaaca gctaataggc ctaaagctgt aagtgcagaa gttgatgaac ctgttgcagg
    18361 aagagtttcc tgctgataaa taagagcata acttacttca ttatcagtag gaattcctgc
    18421 ttgaatcttg gtttgttctt catcaccttt ttaaacttta tttcctaata gtaaacacta
    18481 ttttttattc tcaaaaattg aacttctaaa atcatttgtt tggtcaagat aggctttaaa
    18541 gattgctctc ttcaacttat gaacggcact gtcgatttct ggatcataat tactccaacg
    18601 tggaccttca gcatccttga gaacagcttg gtccatgagg gcttgcaatt catccacatt
    18661 attgatagtc tttgtgccat agcttggcca tggttttgtc ggatctttaa aggtcacctg
    18721 cttcaagttt ccaaactgat ccactcgttc ttggtacata gctgtcttaa attctgccca
    18781 agttttatac ttaccgtcaa ataccttttt caaaacaagc tcatcggtca ccaagccccg
    18841 ttctttacca taaagattga tagtttgccc ttgttgttta gcatcttctt cgtattggtt
    18901 tgagatataa ggtaccattc catccttaaa gcctttggca gccaaaagtt cgtacgcaat
    18961 cctacgtccc ataaggtccc ctggtgtgcc tttctcactg ctgagagctg aatagattgg
    19021 ggcaaagagt ttgatggtat agtagccatt tcgttcatag tcgccagact ggtactcacg
    19081 agctgataag atgttgtggt caatcaaact ttcaaaacta ttcaggtttc gggcctctgc
    19141 ttctgtcaat tctttgacta cgttagtggc ataaacctca tttccatctg ctggatcttt
    19201 cacatacttg ttctcaattt ttctgagagc cgccattttc tggtaaacac ttagtttatt
    19261 aacgattgac tgcccttcga gatattccat catgtagata aggtcaaaca tgttatggac
    19321 atagttctga agatctgctg cgttttggaa tctctctgtc ggatccaaga cttgaagacg
    19381 ggatccctct gtactatctg attttgagtg tttcaagata gaattaatgg taatggttgc
    19441 gtcacttggt tggtcagggg cttgcaataa gccttttgcg aagaactctg gtcccaagcc
    19501 atttcttcga ccatagccac caaggtaaat atcctgatcc gaatcatggg tcatttcatg
    19561 ggtataagta atggctccat ccttgtccag catgcggtaa gacatatagt agacgccgtc
    19621 ccctgtagcg taggctccat gctggttgtg tactacctta ttgccaactg gtccaaagaa
    19681 gttcttcata gctggattgg agctatcaaa cttagcctcc gctgtagctt ttcctgatgt
    19741 tgtgtcatcc ccaaacttat aggcatcata aaggagaatc gtacgataga gtttttcgcg
    19801 tgactgttca tctaaaatac gataccaata atcgtagtga tctcgctgac gtttggctgt
    19861 ttcacgcgca ttttcttcaa caaaatcatt gagagccttg cccgctttat ggtcactact
    19921 gcggtagcga tcataagctc caaatcctag actagacatg gtcgagatga caaatacgga
    19981 tctctctggc aaggtcagga gaggcaagac catattgcgg tatttccatg tggcactcgt
    20041 gatacgatca taaacaccga tagaatactt ggtgccagct aacccttgct tcgttttcac
    20101 ctcttcgata gtggattttt cttcgacaat gtaagcctta gtctctgatt taaaccagtc
    20161 attattgctt gtatttggta aaaagacttt tcggtaatgt tccagcgtgc taaacaaatc
    20221 tgtcgttcca tgttgactgg caagactgat accataagta tcgacattat tcttagctag
    20281 aagattgtta aagccagatt tacctaactc aatcagagta tctagtgggg aagtatttcc
    20341 tttaccaaag aagtctggat gatacataac taggtctttg acattcactt gaccatagtt
    20401 aaagttatac caacgttcta aataagttaa accgagtagc aaggcttcct tattattctt
    20461 gattttatct acaagatagc ctctggtgac tgagttatcg ctagctagac cggcatctgc
    20521 tgacaaaagt tttttcaagc tgtctcccag attttgtttt gttttggaga actggtcttc
    20581 taaatagagc tcggttaact taacttctgg agaaatacca agtgtctttc tgatagcatc
    20641 tgactggtaa tcaagcttct gcaactcagg caagacttcc ttaacaatag agtctcgatc
    20701 atataagaat tggttaggag tgtagagaag tcccgtatta cctagattat attctgctag
    20761 attggcaaaa tcagtctggt acttgagatc aaatttctca gatgaatggt cattatagtg
    20821 aagtaagagt ttgtttgcag tctgtttgtt ggaaacaata tctgtgatta cttggtcatc
    20881 cttcatcata actgcagata agagttcttt ttggtataag agactgttct ccttaaccag
    20941 gtttccgtat ttgacgatgg ttgacttatt ataaaaaggt aacaattttt caatgttctt
    21001 ataaaccaaa gcacggttag cttgataatg ggctatctta gaaaaatcac tgtctttctt
    21061 gccacttgtt gagaggggtt ccaccgttgg tagagtgaga ggatttattt ctgctttttt
    21121 gcttacaatt tgagaagcat ctagcattgt tcctctttct tcaaaggatt ccttgctgac
    21181 gacctcatcc ttctctaaag tgacattgaa aactctatta gccttactac taaatgtatt
    21241 agctaccttc attcctgtat agtggtatcc agtgatggca tttccgtttg ttacattgac
    21301 atcgctaaga acattggtca attcaccagc gtgtttttcc tcaccagaag ttcgatccca
    21361 caaatatcct gccacaccag cgactttacc aaagtgcttg acattattaa tatcaccttc
    21421 cgcataactg ttctgtatat gcgcatcttg gtctactaga cctgcaagac caccgacagt
    21481 ctgatctgac ctattggtgt ttgacgaaat ggttactgtc gctttggatt tagcaataga
    21541 cgcatttttt cctgttaaat gaccgaccag accgccaata ttgtaggcat cagtcgtttc
    21601 ataggtattg acaattcttc ccttgaaact gctctctgcg atgcttgatt ggtctgcctt
    21661 agccaacaaa ccaccgacac cacgttctcc agccagaaca ccatcaacat gaacttgttt
    21721 aatctttgtg ccattcgtag cttcatttgc tagtgaacca atatcatttt tccctgaaat
    21781 agcaacattt tttagactca gtttttctac tgtagcacca ctcaagtttt caaacagagg
    21841 ttttttcaaa ttatagatag cataattctt gccatctttt tcaccgatta aacgaccagt
    21901 aaaggtgtcc ttgatatagg atctttcatc aggaccaagc tccacttcgt tagcattcag
    21961 gctggccgct aaatgatagg ttccagaggg attttggttt atagctttga ccaggttgct
    22021 aaaggaagta aagtttgtat tttcctcttt agccttctta tcgaggtaga aggtgaaatt
    22081 gtcgacatat ttattttcta gttcttgctg gagtttctca gctttggctg tgattttata
    22141 aactgactgc ccatttcttt tctcttcagt tattgaagct actggtatat agacatcttt
    22201 aaatgctgaa gattttacct tgacaaagta agtatccgta ttctctggaa ttccatccaa
    22261 tgaaacatgt tgtctgtagg ttccattcgt ctgactatac aactcaatat cagaaacatt
    22321 tcttaattcg agttttttct ctggttctgt ttgtccattc tctgatgttg tattacctga
    22381 attttctggt tttgttgcag ttccgttttt ttctggttga tttgattctt caactggtgg
    22441 ttttgaatca ctaggtttat tggatacttc tccagtattt tcgttagcta ttttcccaga
    22501 gtttgtttgt gtttcttctg caggttgaac tggtttttct gtttcttgat ttgaggtacc
    22561 ttctactgtg ccttcatttg gatttactgg aacttcttct acagtttttt ctgaattttc
    22621 atttttagag tcattatgtt ctggtttatt tgattctcca actgaggttg tcgaatcact
    22681 aggattactg gacacttccc cagtattttc gctagatgta tctggtgata ctttctctga
    22741 attcgttgtt gattcttctg caggttgaac tggattttct gcttcttgaa ttgaggttcc
    22801 ttctgtagta ccttcatttg gatttactgg tgtttcttct gttggtttta ctggaacttc
    22861 ttcagttttt tctggacctt gttctttggt cttctcaacc ggagtttcag gttttacttg
    22921 ctcaatatta cccttatatt ctggcaatgg ggctacctgc tctggttcac ctttatcact
    22981 taccacagta tctggcgact ctggttgaac ctcagtctca cctttgtcgg tcacaactgc
    23041 ttcgggtaat gtcggttgaa cttctggttc gcctttatca cttactacag cttcgggcaa
    23101 ctcaggctga attgcgggtt caacaataga tccagactgt acgtccttat gttctacacc
    23161 agtctcaggt tgttccttta taacttgagt ttttttagta cctttttcga ctattcttgg
    23221 actaggcgca gtcgttgaag ttgaaacaat ttctcgcgaa acttcttcct tgtttacaga
    23281 gaatattctg acgatttcaa ctttcttacc taatttacct tcttgtttta ctcttacagt
    23341 tccttcagct aaatcaggat tttcttgaat ttcttcttga aaatctattt ttgtctctat
    23401 agtttcctca cgatataaga gttcaggttt gttcaattga cctgataaaa cttcatcctg
    23461 tggatttaat gtatttaccc cagtcttttc ttttggagaa atcttctcct ctttcttcgt
    23521 ttctagattc ttatgttcgg ctaattgttc ttgagaatct gaagattgtt tctcttcttt
    23581 tcttggattg attaattcag tagagaaagg tttttcaact acttgaactt ctgtcggctt
    23641 agttgaagaa acaggtgttt gttcctgaat agcttgtact gttgatggat ggtctacaaa
    23701 atttggtgta acattataat ccaccttttg ttgttttgta ggagtggctg ctgatttttc
    23761 ttgattactt acttcaaaat cagaagtcgt ttttccctct ttgatatatc caatataagt
    23821 gtaacctgaa atctctttag gaagaggtaa tttttctcca gaggtcaatt catagtccgt
    23881 attgtaattt agcaaaagat gattttctaa agcatggact gaaactaaga caccatttcc
    23941 cattcccgca accaatacta aatgtaatac cgttttattc ttaacctttt tcttggaaac
    24001 agcaaaaatt aaaattccca gagcagctaa gctagcacca gcaactaggg cttgcctctc
    24061 attcttgctt ccagtatttg gtaattccgc cagttgattt tgagaattta acttataaac
    24121 aagataataa gtttcatcat cattctccac gtatgtcgga atatcataga caagctgctt
    24181 cttttcttct gatgatagct ctgaatctgc cacatattta tagtgaactc ccgcagtttc
    24241 ttgagcatcc acagatgaac tagctaatac agacataaaa aataaacttg aaatcgttgc
    24301 agatacaagt cctactgata attttctaaa tgaaaaacgc tcttgttttt caccaaaata
    24361 cttttccatt attcctcctt gaaataaaat ttatatatgt tacaaagacc tttattatat
    24421 tagtgtatta tctattatct atagaaaagg cagtatacct taattatact cttaatttac
    24481 aaaaaagtct taaaattgag atgcgctttc atactttgtt ttatattatt tgggggtaca
    24541 ataacaccta ccatgaaatt tacacggtag gtgttactca tatcactaat cgttctaaaa
    24601 atggtttgag gcagttgagg agaattcctt ctatccagct tccttgtgct gatgagcgat
    24661 ggtcttcctg caggcttttt tttagaaaat ctcggacttg ttctggtgcg atttcaaatt
    24721 caaaggcttt cattttatag aaaaagtcga tgagatgatc tgacaggtat tcagttgaaa
    24781 agggtacttc accacttttt cgatattcta ataagagtct agaaaatcga gctttttctt
    24841 caggaagctc acgaaaatag gaattgagga tccaagtctg cttctgtttt ctttcaattg
    24901 gatcctgact ggcaattcgt tggtcttttt ccagctcttt ttggtattgt ttggccttga
    24961 tagctcgttc tgctctattt ttaccaaaaa gaattttttc ccacttgcgt tcttcttgag
    25021 tcagggtctc tgtaaagcca aagtaatctt gataagcacg ctctgcgggt cccatggcta
    25081 gaaccagatt gtctgcatat tgcttggcga ttttatccct cttcttgcgt tctttctctg
    25141 cctggatacg gagttcttgt tcgtagtcaa ttttctcctt gcctagcttg acaaggtaga
    25201 gttggtcatc cgatttccca agtaaaaagg gtttgataca cttttcaagg acttcttcca
    25261 tccgagcctt tttctttggt tccgccttgg tccaacttcc tccctgaaag acttctagga
    25321 aaagctggta gtctctctca ggcgcaaatt gattgccacg attgggtttg aaaacacctt
    25381 tttcccagag ccattttaga agtcgctcgt caaagtcact tttattgacc ttgatttttt
    25441 cctttttctg agcttttctg gttagatttt caacctttct gagcagtttt tcttcctctt
    25501 ccaattgctg gtcaaggggc aatcgatgaa aatgacgaac acagtcgcta ccaattggaa
    25561 agaggcgttg gcctgtgaca ccgttaaaga gttcataagc gtatttgatg gcatttcgac
    25621 agacacaatt gctacggcca ataccgttaa aaatcaagga aacttcattc cattccttgg
    25681 tagcttgttc ccaagtatcc gctttcgaag cctgtaaaac tgcatcgtgc agggattttc
    25741 taactggaag tgtcatgagg tctcctttct aatactcaat aaaaatcaaa gagcaaacta
    25801 gaaagctagc cgcagtcagc tcaaaacact gttttgaggt tgtagataga actgacgaag
    25861 tcagtaacca tatatacagc aaggcgaagc tgacgtggtt tgaagagatt ttcgaagagt
    25921 ataagttata cttttacaac ttgaacctcg tctttaccga gtaaaatcaa gtatttttca
    25981 atattttcaa tcgaataggc tcgtgataaa gcctcttcgt atagagctaa ctgaccacga
    26041 tagcggtcta cgagttgact tggttcatca tagcggtctg tcttgtagtc gaacagaaca
    26101 attttgtttt cgtaaagcag atagccatca aggataccac ggacaacaaa gtcttcctga
    26161 ctcttttggt ctcgtttgag catggagaaa ggttgctcgc gatagagatg gtcggtatta
    26221 gcaagaattt cctgaccgag tgctgtgtca aagaaagcaa gaattttatc aagattgatc
    26281 ttgtctctga cagcttggct agtttgaact tgtttgagtg tttctgttaa gctagcaagg
    26341 gttagttgct ggctgaggtc aattatctgc atgagttcgt gagtagcact accaatctca
    26401 gctccagtta ccttttcttt ggttgaaaaa tctggcaaat cgaagctgat tttcttgcct
    26461 actgactgac cttgaccagc aatctcgaca ccttccatat ccataactgg ttcgtagaat
    26521 ttcttgattt gacttggggt ttgaacacta ggaagttcaa tagctgcgcg gtgaagagta
    26581 ttataaactt ccacctcctt cagcatttcc agagcttctt tgatggtatc tgactgacga
    26641 ttgtctgctt gggagctatc ttggagagga ctcttggttt ccaactctcc gatagcttct
    26701 ctggtcaact gatcttcgtc aataaaacga taactaaagt tgagcttgtc cttagtaaac
    26761 actttactga tagcccaaag ccaatcttgg aaattccgtg cttgcagtct agtattgcta
    26821 tttagtttcc catttttggc tgctgggtat tccttggatt ccagcttttc acgagaaccc
    26881 ttgccgacaa gatagagctt tttctcagcc cgcgtcatag caacatacag caaacgcatc
    26941 tgctcagaat agcttgctag ctgtaattcc tcttcgttct gcctataggt cagactagga
    27001 atggagagtt tgatggtttt aggatagtgg tcttctactg cccctgtctc catcttggca
    27061 atatatttga caccaagacc attctgacga ctgagaatga cttctgacat agagtcttgc
    27121 ttgttgaaat cttgatccat attgaggata aagacgtaag gaaactccag ccctttactc
    27181 ttgtggatgg tcatgagctc tactgcatct tttggcggtg cgacggccac gcttgccaaa
    27241 tcgtgctggg cttctaagac ttggtcaatc atacgaataa aacgcgacaa acctttgaaa
    27301 ttgctctttt caaattgatc agcacgcagt gctagggcat agagattggc ctgcctagca
    27361 ggaccattcg gcaaagcccc aacatagtca taataaaaac ggtcgttgta aatcttccaa
    27421 atcaagtcat agagagagtg ggttttggca tacaagcgcc aagaagctag gatatccatg
    27481 aattgcttta gtttttcaac tagagctgtg tgaatcaagc ctttttgact acttgccatt
    27541 ttttgtgcat tgaccagttt ctcatagaga ttttcgtgga ctttatcctc tgctttctga
    27601 agggacaaac gtgctagctc atcctcatca aaaccaaaca ttggagactt cataagggca
    27661 accaaggcgt agtcttgcag gggattgtga atgacacgaa gagtgtctag catgacttgc
    27721 acttctaggg attggagata attgttttgc tctccgtcag ttttgacagg aatcccgtac
    27781 tcagacaggg cgaggagaat ctggtcatta cgactgcggc tggaggtcag aagggcaatt
    27841 tccttaaagg caacaccttt ttcttgatga agtttcagaa tctccttgat aactaagcgc
    27901 atttcgcctg ttagtttcgt ttctgtttga ctctcttctt cctcacctgt atcgtccttg
    27961 tcgtagagga gaaattctgc cttgttgtct ggattgggag tcagtttggt attggcaaaa
    28021 acaagctggt gcttgttatc atagttgatt tcaccgacct cttggtccat gagacgttca
    28081 aagacatcat tggttgctga cagcacttct gaactactac ggaaattttc cttgaggaga
    28141 atgagcttgc cttcttgggg attttgcgca tagcgttgga atttctcatt gaaaatctgc
    28201 gggtctgcct gacggaaacg atagatggat tgcttgatat ctcccaccat aaagcgattg
    28261 tggccattag acaacaattc cagcatccgt tcttgaatat ggttggtatc ctgatactca
    28321 tcgaccatga cttcatggaa gcgctcctga taagactcac gaacttgtgg gaaattctct
    28381 aaaatctcaa tggtgtaatg gctgatatca gcgaattcaa aggcattttc ctggcgtttg
    28441 cgctgacgat aagcctccac aaaatgactc ataaaggttt ggaaggtttt agctagtttc
    28501 catgtgtctt gatgataacg ttcttgatag tcgagaatgg ttatctggtc tgataattgt
    28561 cctagtttag caaactgggt ctttctctct tcgttgtagg catcagccag gggcttcaaa
    28621 tcagccttac gactggcatt agtcagagct cgaccgtttt tctccttaga gatggcgaca
    28681 acacgcgcaa gcactgcctg ataagcctga ctatcggact cctgatttag ggagcaaatt
    28741 tcatccagaa ttaactgaac attttctaaa taggcagcct ttgcaaactc cttggcatcg
    28801 ttatccagat ggtaacggaa aaagctttcc aaatcccaaa gggcttgttt gatttgctca
    28861 gtcagttttt ctttttcact ggtaaaatca gctttctcaa atcctttgag gaaagattca
    28921 ctcagccact tttgaggatt actggtggat tggaggaagt catagatttt atagacctgc
    28981 tggcgcagac cccgttcgtc cttgccacgc ccagcaaagt ttttcagcaa atgactaaag
    29041 gtctctttct gtttaccttg gtaatgcgct tcaaagacct catgaaagac ttcgttttcg
    29101 agaagaagtt gctcgctttg gttttgtaaa atacggaaat taggtgcaat atcaagcaga
    29161 taaccatgtt tgccaaggaa tttttgtgtg aaagagtcca tggttccgat ggcagcgttt
    29221 ggtaggtctg ccaactggcg tcccaagtgt tgtttgaggt cgacatcatc tgtttcttgg
    29281 attttcttgc tgattttttt ctctaaacgt tctttaagtt cagttgcagc cttgacggta
    29341 aaggttgaga taaagagttg agaaatttcg acaccacgcg ccaattggtc cagaatgcgc
    29401 tctgccatga caaaggtctt tccagaacca gccgatgctg agaccaggat attctgacca
    29461 gaagtgtaga tagcttcgat ttgctcggca gttttctttt gttccttgct cgaatttgct
    29521 tctgcttctt gcagtttttg aatctcctcc tcacttaaaa agggaataag cttcatcgat
    29581 tcaactcctc tcttattttt tcaagccaag cttgcttgag tttttctccg accagacgct
    29641 tgccatcagc taggtccaac ttttctagga aacgggcttg gcccagatgg taattggctt
    29701 caaagcctgt aatagcctga tgttgctgga cgtatggggc aatgcttctg ccattttcag
    29761 tataaggatt gatggcgaac cggcctgcta aaatcttctc agcagctttc ttgtaaagat
    29821 aggcattgta gtccagtagg agctgaaatt cctcatctgt cagttgattc gccttgtttt
    29881 tgttataaaa ttcgcctaaa taactgcttt ctttttccaa gaagagccct tggtatttca
    29941 tagatttgct ggcttctacc actgctcctg ccagactttt taccgccatc agagattgga
    30001 caggttcagc catttccaag tacatggcgc cgaaaaagtt ctgctcccct tctcttttta
    30061 gggcagcaag ataggttggt aactgagaat tgagcccatt aaagaaatga ggaaactgga
    30121 actgagtcag actggatttg tagtctacta ctcctatcgc tccattagct ttcaaacggt
    30181 caatccggtc caccttgcct cgtacaaaga cactgcgtcc attgtctaat tgaataaagg
    30241 cttggtcttt tccaccaaaa tttgcttctt ctttgattgt ttcgatggct ggattgtgtc
    30301 ggagaatatg tccagttgtc cgtgcaacat caagcaaaac ttccttggta aactgggctt
    30361 ccaaactttc ttgataaata gcttcaaatt cgcgttcttg actggtttct tgaatagctt
    30421 gttctagacg ttggtcaaag gaatcttcat taggcaactg caaggcgcgt tcaaagatac
    30481 gatgcaagaa attcccgtga ctacgggcat caggacgcaa acgtaattcc tcctgcaagc
    30541 ctaaaacgta gcgtaggaaa taactgtatt cattgcgata aaactctgtc aaacccgacg
    30601 tagacaggta aaactcctgt ttggcaggat agagagcttg caaggtgtcc ttggctaagg
    30661 tcttgctgct tggactgatt gggatggctg gattttccag accttgctga tctagttttt
    30721 tacctatgac acgcgacaga accttgacaa aagtcaaatc ttgctcagta tcgctcatct
    30781 caccctgctg gtgataggca accagactag acaaaagact gtgataggac cccatatcct
    30841 ccttagacag tcctttgtga ttcatcctct tctctctccg cctaaatcca aaatggatca
    30901 actcttgaag ataggcagat tccttacttt cactttcgtt aaaaaggctt ggagccgaca
    30961 agaacaactg cttacgagca gaattgacca aggaaagcat agtgtagcga tttttcttga
    31021 gattttcact gctggcaatc agtaattgaa cgccttcttc ggtcgtttgg tttaggtttt
    31081 gcctttcttc atctgtcaga agactggtgt tttgagaaat ttttggtaaa ttgtcctgag
    31141 ttagtccaat agcatagaca aagtcagcag tcaatggtgc aatcaaatcg taactctgca
    31201 ccagaacagt gtccactgtt gctggaatgg tacggtattg ggacaaactc attccagaat
    31261 ggagcaaggc taggaagtct tccagactaa cctgtgaacc agcaaaaaca gtcgcaaatt
    31321 gttctaaaac atggcagaaa gccttccaaa cttcggcttg tctttcctgt tctacagctt
    31381 ccaaagtggt tgtcaaatct tgtaactgct tggtcacagc tccttctttt agaaagacac
    31441 tccatttttg taggaggttt tcagcctttt gttttcggct ggcaaagagg gtttcaagag
    31501 gtgctaaaat tctcaggcgg aggacattca aacgctcaag attaaatttt ccatggtggg
    31561 atttggtgaa agtttgctga aaggctggca agccattgat accaagatag cggatatatt
    31621 gctcaaaagc atcaatatca gactgactga ggtcagtata caaatcagtt ctaagaagat
    31681 taatcaaatc ctcctgacga aaacggtaac gttttaaagc taaaatagac tcgacaaact
    31741 gagtcaaggg atgatgagcc atggcttcgc ttctaccaag ataaaaagga atctgatact
    31801 ggtcaaaaat ggttttgaga gataactggt aagaagctac atcccccaag agaatacgaa
    31861 aatgcttgta gctcaggtct gagttctcat gtaatttctg acgaatgcta cgggctacta
    31921 gctccaactc ctccttttgc gtcaaacaag accagatttg taaattttca cggtctttct
    31981 catcgacatc caaagcgagt tctgaaaagt cataagaaga ctccaacaaa cgagaggcct
    32041 tgtcaaaact atccatcttc tcatgagttt gagaacagtc ctgagcaggc gtttggtatt
    32101 tagaagccag atgatggaga aattttacgc tggcttggta gagattgccc tcgctaaaag
    32161 gactggtata ggctttctta ctagcataag ccccgataac aatctcaaca cctttgccgt
    32221 gaagtaagtc cacaacccgc tcttcctcag cagaaaaacg agtaaagccg tcaatgacca
    32281 aggtgatttg attaaaatca ctacttacct tgtcattctc aatagcctca atcaaatggg
    32341 acaactgact tccctgggct aactgacctt gattaagata ggctgttact ttctcaaaaa
    32401 tcaagagtaa atccgccctc ttatcctcat ctgttaaatt ctccaagtcc aaaaaactca
    32461 tctgagattt ggtcatctca tggtaaagct caattaactg ctggatcaat tgaggatcct
    32521 gcttaatagc gccataaaca cgcaagtcct tgggatcgag ttcggcaagg catttgtaaa
    32581 aggccaaccc aagaccgata tcatcaagag tagttttaac tggtaaatca ttcaagacca
    32641 gataacgagc catttgagca aagcgcgtga cggtaatcga aaaagaagcc tgctgggaca
    32701 agcattccag cacggcgcgt tccttttcaa aagaaagaga gttgggggca atgtagaaga
    32761 cccgcttgcc agctgcaact agctcttctg cctctcttgt tagaatttct gtcaaagaag
    32821 ttcgaatatc agtataaagt aatttcatct cagcctcgtt ggaatttttc atcaccctat
    32881 attataccat gattagcctc gtaaatctgt taaaatattt aggccatcct ttcttttctt
    32941 catcatctgc taaatcttaa atacttagct ttacttgtat tagatagaat aagtctggct
    33001 actgaaaatc acataataaa aaagcctcgg taacaaggct ttgagtttta tgattgtttc
    33061 ttaggtacgg aatacacttc aatgtgttgt cccagtatct taatgtcgac tggtagattg
    33121 tctgatttat cgccatcaac atcggactct aattcgatat cagaagaagt tttaatatta
    33181 cgtgccttta tatattcaat attcttgata gaatgattga actatagtaa attgaaacta
    33241 taatagtaca ccgtggatgc taaaatattt ctagaaatta atttgatttc cctaatcaag
    33301 ctgttcgtat cttatttcaa tcta
//
LOCUS       DAVBCB010000019        33055 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVBCB010000019 DAVBCB010000000
VERSION     DAVBCB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33055
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.019"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(599..1288)
                     /gene="rplA"
                     /locus_tag="VQU28_000864"
     CDS             complement(599..1288)
                     /gene="rplA"
                     /locus_tag="VQU28_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HET2476241.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMGLVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(1495..1920)
                     /gene="rplK"
                     /locus_tag="VQU28_000865"
     CDS             complement(1495..1920)
                     /gene="rplK"
                     /locus_tag="VQU28_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HET2476242.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAVRMIEGTARSMGFTVID"
     gene            complement(2043..2759)
                     /locus_tag="VQU28_000866"
     CDS             complement(2043..2759)
                     /locus_tag="VQU28_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HET2476243.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGAAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     VIGTNGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYFGFEGGDYVD"
     gene            complement(2762..3232)
                     /locus_tag="VQU28_000867"
     CDS             complement(2762..3232)
                     /locus_tag="VQU28_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2476244.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVSELHHLEKKTRIRFLEEMSVVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGYGLNGRGPVWWIPFEEMTAEICQSKPDEIKRLVERLSSEVDKLLEIKE"
     gene            complement(3234..4271)
                     /locus_tag="VQU28_000868"
     CDS             complement(3234..4271)
                     /locus_tag="VQU28_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HET2476245.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGQKVLGTILIHQTSCHVYKDAGTAERTQDNMEVRLDVKVSNEKETRALGIEVG
                     DFISFDPRTVVTDTGFIKSRHLDDKVSAAMLLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTNEYTVSICVKDASGPYHYDFRQHLVALA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSALVD"
     gene            complement(4369..5694)
                     /gene="brnQ"
                     /locus_tag="VQU28_000869"
     CDS             complement(4369..5694)
                     /gene="brnQ"
                     /locus_tag="VQU28_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HET2476246.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(5865..5957)
                     /locus_tag="VQU28_000870"
     CDS             complement(5865..5957)
                     /locus_tag="VQU28_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2476247.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            complement(6000..6152)
                     /locus_tag="VQU28_000871"
     CDS             complement(6000..6152)
                     /locus_tag="VQU28_000871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HET2476248.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LADSYD"
     gene            complement(6312..6890)
                     /locus_tag="VQU28_000872"
     CDS             complement(6312..6890)
                     /locus_tag="VQU28_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HET2476249.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPCT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(6944..8344)
                     /gene="pepV"
                     /locus_tag="VQU28_000873"
     CDS             complement(6944..8344)
                     /gene="pepV"
                     /locus_tag="VQU28_000873"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HET2476250.1"
                     /translation="MIAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLTDLQAKLDAFVAEHKLRGELQEEAGQYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(8357..8962)
                     /locus_tag="VQU28_000874"
     CDS             complement(8357..8962)
                     /locus_tag="VQU28_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HET2476251.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDLKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYNEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDDKLEPSYRLPVDEIIEKR"
     gene            9133..9933
                     /locus_tag="VQU28_000875"
     CDS             9133..9933
                     /locus_tag="VQU28_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2476252.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            complement(10115..10948)
                     /locus_tag="VQU28_000876"
     CDS             complement(10115..10948)
                     /locus_tag="VQU28_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2476253.1"
                     /translation="MNHKIAILSDIHGNATALEAVIADAKTQGASEYWLMGDIFLPGS
                     GANDLVALLKELPITASVRGNWNDCVLEALDGQYGLEDPQEIQLMRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(10938..12785)
                     /gene="uvrC"
                     /locus_tag="VQU28_000877"
     CDS             complement(10938..12785)
                     /gene="uvrC"
                     /locus_tag="VQU28_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HET2476254.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGIHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGRPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMRHFKSLTKIKEA
                     SVDEIVEVGVPRAVAEAVQRKLNPQETEILLQVAEERVDYQTEGNHNKP"
     gene            complement(12825..13526)
                     /locus_tag="VQU28_000878"
     CDS             complement(12825..13526)
                     /locus_tag="VQU28_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HET2476255.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKMGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(13605..14837)
                     /gene="murN"
                     /locus_tag="VQU28_000879"
     CDS             complement(13605..14837)
                     /gene="murN"
                     /locus_tag="VQU28_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HET2476256.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQGLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(14842..16062)
                     /gene="murM"
                     /locus_tag="VQU28_000880"
     CDS             complement(14842..16062)
                     /gene="murM"
                     /locus_tag="VQU28_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HET2476257.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(16174..16953)
                     /locus_tag="VQU28_000881"
     CDS             complement(16174..16953)
                     /locus_tag="VQU28_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HET2476258.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17022..18683)
                     /locus_tag="VQU28_000882"
     CDS             complement(17022..18683)
                     /locus_tag="VQU28_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HET2476259.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPEHIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKILSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEDYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(18858..21080)
                     /gene="recJ"
                     /locus_tag="VQU28_000883"
     CDS             complement(18858..21080)
                     /gene="recJ"
                     /locus_tag="VQU28_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HET2476260.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQDQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKTYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            21357..22115
                     /locus_tag="VQU28_000884"
                     /pseudo
     CDS             21357..22115
                     /locus_tag="VQU28_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            22155..22919
                     /locus_tag="VQU28_000885"
     CDS             22155..22919
                     /locus_tag="VQU28_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HET2476261.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDAFSVDRSILSGYTSKRTELLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            22932..23609
                     /locus_tag="VQU28_000886"
     CDS             22932..23609
                     /locus_tag="VQU28_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2476262.1"
                     /translation="MIDLSSWTAYFQDFGQFFNGFLFTLALAIGSFILAMVLGIFFGA
                     MSTSKRPILRILAHIFVEFYQNTPLLVQFVIVFYGLPLISDHTIMIPIYWTAVLCMGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQTFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            23619..24278
                     /locus_tag="VQU28_000887"
     CDS             23619..24278
                     /locus_tag="VQU28_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2476263.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNTVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLCILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            24573..25763
                     /locus_tag="VQU28_000888"
     CDS             24573..25763
                     /locus_tag="VQU28_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HET2476264.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHGLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKNSGDHTNNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26084..26965)
                     /locus_tag="VQU28_000889"
     CDS             complement(26084..26965)
                     /locus_tag="VQU28_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HET2476265.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27106..28434)
                     /gene="vncS"
                     /locus_tag="VQU28_000890"
     CDS             complement(27106..28434)
                     /gene="vncS"
                     /locus_tag="VQU28_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HET2476266.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGATMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            complement(28431..29087)
                     /gene="vncR"
                     /locus_tag="VQU28_000891"
     CDS             complement(28431..29087)
                     /gene="vncR"
                     /locus_tag="VQU28_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HET2476267.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASIAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(29136..29219)
                     /gene="pep27"
                     /locus_tag="VQU28_000892"
     CDS             complement(29136..29219)
                     /gene="pep27"
                     /locus_tag="VQU28_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HET2476268.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(29399..30778)
                     /gene="vex3"
                     /locus_tag="VQU28_000893"
     CDS             complement(29399..30778)
                     /gene="vex3"
                     /locus_tag="VQU28_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HET2476269.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(30830..31477)
                     /gene="vex2"
                     /locus_tag="VQU28_000894"
     CDS             complement(30830..31477)
                     /gene="vex2"
                     /locus_tag="VQU28_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HET2476270.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQIDYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(31490..32767)
                     /locus_tag="VQU28_000895"
     CDS             complement(31490..32767)
                     /locus_tag="VQU28_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476271.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNGITSYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9331 a         7575 c         6147 g        10002 t
ORIGIN      
        1 gtattagaaa gctcgatgga ctattccaga ttcattccac tagatttcga aatcgagtag
       61 gggataatct ctagcccctc tcacaccacc gtacgtgccg ttcggcatac ggcggttcaa
      121 ctaactgtta acgcatgtcg ttcaaggtaa taatccaaac acgaaaccag tccacgtttt
      181 tcaaggactg gttttgatat agcacgttta agtaccgact tctgagctac tatagtagat
      241 tgaaactaga atagtacatc tcttcttcta aaacattgtt agaaatcgat ttggctgccc
      301 tgatcgattc gtcctactct tatttcattt tactataatt gatagtggtc gccccagcca
      361 gataccttat ccgctatata ggccttatta tcatgttcct gttcgtagag ccacgatttc
      421 gctatccctt cctctgacga ttcttatttc ctgcgtcaga cttgggaatc gctattgggt
      481 tcaccggtag caggtgccca ccgaggactt acacctcaga tatacgacat gcccgtcgta
      541 catcaaaaaa agttgagaca tagcgtcttc aactttttta cattacttta actactgatt
      601 aaagtgagtt tacgtcaact ttgataccaa caccttgagt agttgtgata gtcaagtttg
      661 ttacgtaagt tcctttagct gtagctggtt ttgctttttg gattgtttcg ttgaaagctt
      721 taaagttttc aaccaatttt tcagcttcaa atgatacttt accgatgatt gcttgaacgt
      781 tacctgcacg gtcagcacgg taagtgattt taccaccttt agactcttca actgctttag
      841 caacatccat tgttacagta ccagttttag ggtttggcat caagttacgt ggtccaagga
      901 cacgtccaag acgtccaaca agacccatca tatcaggtgt agcgataact acgtcgaagt
      961 ccaaccaacc gtcgttgatt ttagcaacaa ggtcatcttc accaacaaag tctgcaccag
     1021 cagcttttgc ttcttcagct tttgcaccac gtgcgaaaac aagaacgcgt gaagttttac
     1081 cagtaccgtt tggcaatacc attgctccac ggatttgttg atcagctttt ttaacgtcga
     1141 tgttcaagtt gtaagcaact tctacagttg cgtcaaattt tgcaaagttt gtttcttttg
     1201 caagtgctac agcttcttct acgctgtatg cttttgtgct gtcgattttc tcaagagcag
     1261 cacgaagttg tttgcttttt ttagccattt tctattctcc ttgtaagtgg ttcaatcgat
     1321 tttcatctcc cacgtcactt ctcgaaatga tgaagtcttg cgggttttca gtctattaga
     1381 ttcttttcct acggccttaa gctcccttgc tgtacccaag tacaagcatc agtcacttgc
     1441 ctagtatgaa aagcaactaa tagactgctg tgagattttc tactgtgtta ttgattagtc
     1501 aataacagtg aatcccatag aacgagcagt accttcgatc atacgcactg cagactctac
     1561 gtttgctgcg ttcaaatctg gcatcttagt ttctgcaatt tcttgtactt gtgcacgagt
     1621 aactgtagca actttagttt tattaggtgt acctgatcct ttttcaacac ctgcagcttt
     1681 tttcaaaaga acagcagctg gtggtgtttt cgtaacgaaa gtaaatgatt tatcttcgta
     1741 aactgagata acaactggaa tgatcatacc agcttggtca gctgtacgag cgttgaactc
     1801 ttttgtgaat cccatgatgt tgataccagc ttgaccaaga gcaggtccaa ccggtggagc
     1861 tggtgtagct ttaccagcag ggatttgcaa ttttacaagt ttttcgactt ttttagccat
     1921 ttttaaatcc tcctttgtgg ttttggcggt aattagagat ttttacctcc cacaagtatg
     1981 tttttcacat acccatctat tatacactat ttatctggaa ttgcaagaga aaagtttatt
     2041 ttttaatcga cgtaatctcc accttcaaag ccgaaatact cttccaaact gaggccacta
     2101 gctgcaatct ctttagcctc ttttccgaca taacgaaggt gccattcctc agccatatag
     2161 cctgtttcct tttccttacc tttgagataa cgtacaacaa agccataatc agccgcatga
     2221 tccaagagcc attgggccgc tttttcttct gtcaccaaat caccatttgt cccaatcaca
     2281 tcaaaggcta aacctgtctg gtgctcgcta tagccaggac gggcagaata acggtcggca
     2341 gcttctttcc catcttgatt gacataatct tgatagagct tggtctgagt ttcataactt
     2401 ctaaagccac tgtaatgatc gctaatgggg aaacctgcct cttgcatagc tttgatgagc
     2461 ttgaccaact cagccttggc tgttggattt tcccctggat tgtagtcttt tgacaatgga
     2521 tagtgtttgt tggctacgat gatttcatcg tatttccctt gaatgctgta atagtctcct
     2581 ttgttaacca cttctgcttt tttctgggca gctccttgag acttacttcc tactgttcca
     2641 tcagctttag cagtttgctc tgtcttagct gctccatctt cattttttgc tttttcttgt
     2701 gaacaagctg ctagactcaa ggctagcaag gctgtcaaga caaggtatct ttttttcatc
     2761 tttactcctt tatttctaat agtttatcta cttctgatga taaacgttcg actaatcttt
     2821 taatctcatc aggttttgat tggcagattt ctgctgtcat ttcttcaaag ggaatccacc
     2881 atactggacc acgtccattg aggccataac cattcatatc acctcttcgt ctaggaaaca
     2941 gatgccaatg gagatgagca tcgccatttc ctagtagttc gatattcatt ttctcagcag
     3001 caaaggcctt ggcaactgcc tcttggacca cactcatttc ttctagaaaa cgtattctag
     3061 ttttcttttc taaatgatgc aattcgctga catgttcctt ggctagaaag agactatagc
     3121 ctgcaaaata ctggtggtct ccaatcacaa gatagcctgt ttccaactct ttgacaaaat
     3181 agggattttc tcccttcttg atgagctcaa ttctctggca aatcaaacac atattagtct
     3241 accaatgcac tcttaagata ggcatcgacc attcgttctg ttgcgaccac cgagtcaata
     3301 tgagtgcgct cataagaatg actagactcg atacccgcac caagaagggc atgcttgacc
     3361 tctgcccctg cagacatagc cgctgaagca tccgaaccat aaaatggata gatgtccagc
     3421 ttaaaaggaa tatcttgctc tttagccaag gcaaccaagt gttgacggaa gtcatagtga
     3481 taaggacctg aggcatcctt gacacagata gatactgtgt actcatttgt ctgctggtca
     3541 tctcccatag ctcccatatc cacagccaga tattctacta cctgagcagg aatgttagaa
     3601 ttagcaccat gtcctacttc ttcaaaaact gaaaaagcaa aatgagttgt tactggcaat
     3661 tcaatcttct ctttcttata aatacgaagc aggttgagca acatcgctgc gctgacctta
     3721 tcatccaaat gacgagactt gataaaacct gtatcagtca caacagttcg tggatcgaag
     3781 ctgataaaat caccgacctc aatcccaaga gcacgagttt ctttttcatt gcttactttt
     3841 acatccaaac gcacttccat attgtcctgc gtgcgttctg cagttcctgc atccttgtag
     3901 acatggcaag aagtttggtg gatgaggatg gttcctaata ccttttgacc tgtgctagcc
     3961 acatgaacgg tacagttttc accttcaatc atgttccaag gaaaaccacc gatacggtcc
     4021 aatttaagac gaccatctgg tttgacagca cgaacaatag cacccagtgt gtccacatgg
     4081 gcagtcacat agcgatgttg ctcatcattt tgacctttga tggtcacatt gacaccaccc
     4141 ttggctgtgc gaactggctg gtaaccaaaa ccttctagag tcttgactaa ataattcgaa
     4201 atttcacgag tgaagcccgt tggcgatgcg atggctgtca gttctttgat atattctact
     4261 gtttgattca tttcttcacc tctttcgcta cctattataa cacatttatc atcggataaa
     4321 aaaagaaaat cactcctgta gacttggcta caaaagtgat ttaagtgatt attccatttc
     4381 aaaaacatcg ctttcttgct tgtttggtag aaccaatgag agcaagattc caacaatggc
     4441 tggcaccaac catgggagag atgccttggc aaaaggaaga acgctaacaa gatttgcaag
     4501 aaactcaacc ttaaacgagc ttcctagtac gcttgcaatg gcaataactg taaccacagc
     4561 aattgtcaac tgcatacctg gttttgaaag ggcaacaaat ttgttgacaa tgacaatcat
     4621 aacgatagca atcgtgattg ggtacaagat aaccagtact ggaattgagt acttgataat
     4681 cgcatcaaga cccaaattgg caatagcaaa tccaatcaag gtaaaggctg tcgcataaac
     4741 cttgtagctg atttgtggga agcgctcatt aaagaactca gctgttgaca caatcaaacc
     4801 aacagtcgtt gtgaagcagg ttacggtaac catagctgca aggaagagtt gagctgttga
     4861 gccaaagatt tcttgagtgg cttgtgacaa gatgtaaaca cctggtgttc cacccttcat
     4921 cgcttcagct ggtactggga aatgatttcc aagaaaacct aaaccgatgt aaagagcgct
     4981 gaaggcaagg gcaacaacga taccaacaac ccaaatagtt gaaatgtatt ctttcttact
     5041 tgaaaatcca agttgtttca aggtttgaac tgcgattacg ctaaaggcca ctgaggcaag
     5101 ggcgtccaag gtattataac cttctaggaa acctgtacca aaggcagaag cttgataagc
     5161 agctgaagca gcttgaggac ttgttccacc atatttgata gctcccagaa cgaccaagat
     5221 aacaatcaaa attgcaaaga ctggcgttaa aatacgtcca atgcggtcta agatttttga
     5281 tggattaagc gaaatcaaat aggctgccgc aaaatacaga accgtaaata caatcaagcc
     5341 aagtccttta tttgcatccg acaaaagggg gctaatccct acttcgtaag ctgttgtagc
     5401 agtacgtggg gtagcaaaga atggaccgat tgacaagtaa agaactgaga ggtaaagagt
     5461 cgcaaaccaa ggcgctatct tcgttgaaat ctcgtagata tatcctttag gatttagcgt
     5521 tccaataata agggtcaaga cggcgatacc aacgcctgaa aagacaaaac ctgcgatggc
     5581 aggaagaaaa tgttctccag atagagcacc tagagaaggc ggaaaaatca agttccccgc
     5641 accaaaaaat attccaaaca ggagcaaacc tgttagggta ccttttttag ccataaaaat
     5701 ctccttcata tttataaaat actgatctag tataccacga ttaggagtct gaaaaaagca
     5761 tcacgaggca aatattaaat gttttcaatt ttcttaaaaa taagaattat ctaaaaataa
     5821 aactctccta cccagtccac accaagtagg agaaatgcaa aattttaaag ttcttcaaag
     5881 gctaacatgc caccctggac attgataaca ttataacctt gttctaatag gaattggcaa
     5941 gcacgcgccg atctcattcc agatttgcaa ataatataat gcaagcgatc tttgtccaat
     6001 taatcataac tatcagccaa ttgactaagc ggtaggttgt gggcaccttc taaatgaaga
     6061 gctgcaaact catccacttc tctcacatcc actagagaaa gctggtcgtt ttggtaaagc
     6121 tggtaaaatg cgtcaaaggc tatttctttc attctttttc ttctaatact cttcgaaaat
     6181 ctcttcaaac cacgtcagcg tcgccttacc gtgcgtatgt taccgacttc gtcagtttta
     6241 tctgcaactt caaagctgta ctttgagcag cctacgacta gcttcctagt ttgctctttg
     6301 attttcattg actataaaat ggttttaatt ctttttttca aatctggcac tacttctgcc
     6361 tcaaaccaag gatttttggc catccagatt tgatttcgtg gtgatgggtg aactagcgga
     6421 aaataggctg gcagatagtc tttatagtgt ttcaccctct ccgttacctt cccactgatt
     6481 ttctcctgta aatagtaggc ttgggcatat tgcccaatca agagggttaa ctgaatatca
     6541 ggcaattcct gtaagagctg cggatgccat ttttctgcaa aacctgtaca aggcggaaga
     6601 tcacccgact tgccatgtcc tggaaagtag aaatccatag gcaaaacagc aaaataacct
     6661 gaattgtaaa aggtatcttc atccacacct agccagtccc gcaaacggtc accactttta
     6721 tctttccagt aaaggcctgc ttcttgagtt ttaagtcccg gagcctgacc gatgatattg
     6781 atgcgagcag tttttggcgc tgcaaagaga ggctcaatgc cacgctctgt atagctggca
     6841 ttctgcgaat ccgccatgat agcctgtttg attctttcga tttgagacat ctactttccc
     6901 tccaaaaaga agtccaagca taagatctca gacttctatc gttttatttg atcaattcgt
     6961 aaatagcttc ggcataaatt gctgctgctc ggaagagatc gtccaaggca ataaattcat
     7021 tggcttggtg catggtatca attgagtctg ggaacatagc accataggca actccgcgct
     7081 ctaacaagcg accaaaggtt ccaccaccga tgacttgttc atgaccttta aggcctgttt
     7141 gtttttcata gacattcaac aaggtttgca caagtggatc ttccattggc acatagtgag
     7201 gggtatgacc atgttctgat agactgacag aagcaactgg caagttttct aggattgact
     7261 tgatttgttc tggacttgtt ccttttggat agcggatgtt aagggcaatg gtattatcag
     7321 cacttgtttc atcgaagcgg aagacgccgg cattcataga aagagcaccc atcttttcat
     7381 ccacatgagc aatcttgaga ttttcaccct catgatcgtt caagagaatt ttacctgcga
     7441 tgtcaaggta gtctttggct ggaccagcaa agccaaactg gctgaggaag agggcaaggt
     7501 aagttgcgcc attgacacct gaagcaggca tagcaccgtg ggctgattta ccaatgatcg
     7561 tcaccttgta ttggccagct tcttcttgga gttctcctct aagtttgtgt tctgcaacaa
     7621 aggcatctag tttagcttgc aagtctgtca agtcacctga aacgactgct gttgctgatt
     7681 ctggtaccat attttcacgt aaaccacctg taaagctgtg aagacgggct gcacctgtat
     7741 tttcacctgc aaagtggagg tattccgtga tgtttccttt ttcaccattg atgattggaa
     7801 attcagcatc tggtgagaaa ccaaagtctg gcttggcgag tcctacgtgc tcgaagtagt
     7861 agtccatatc tgcccagcct gattcttcgt ctgttccaac gatgaagcga actttcttag
     7921 aagttggaag gcccaattct ttgatgattt tcaaaccata gtagcaagct gttgtaggtc
     7981 ccttgtcatc tgaagcacca cgcgcataaa ggcgaccatc tttgatagtt ggtgtgtaag
     8041 ggtctgtgtc ccaaccgcta ccagctggaa ccacgtccat atgggcaaag attccgagaa
     8101 cttcttctcc atcaccaaac tcaaaatgtc ctgcatagtt atcaacattt ttagttggat
     8161 aaccatcacg gtctgcgatt tcaaggaatt tttccaaggc ttttactgga ccaggtccaa
     8221 atggatgttg ggcatcagcc ttgctatcat cacgttctga gttgatttcc aaaaggctaa
     8281 acaagtcagc caagaggtct tctttgcgtt tttctacttc tgctgtaaaa tcaattgcta
     8341 tcattttttc ttcttcctat cttttttcga tgatttcatc tactggcaag cggtagcttg
     8401 gttccaattt gtcgtctgtg taacccactg tgatcaagag ttctgggcgg aaacggtctt
     8461 caatttccaa aacctcattg acttttgatt tgtcaaaacc aaggataata ttggaaccaa
     8521 ttccttggtc tgtaagagcg agaaccaagt tcatagcaac caaacctgca ttgagtgcta
     8581 ggtagtcgct gacttgttgt tcattgtagc gtgcaaattc agctggcaaa tttttcatga
     8641 agtattgaag ttgttcttct gagaaattgt tagcaccacc aactcgggca atcttacgag
     8701 cgcgtttggc aaggtctgta tctgtaaata gggcaatggt tacaggtgct gatgatacct
     8761 gctcaaagtt tgaaccataa gccaactttg ccagttcagc atttttctca cgaacaacta
     8821 caaatttcca aggctggctg ttgtgggcgc ttggcgccaa ggttgcaatt tcgatagccg
     8881 tacgcacatc ttttagatca acaggcttat cagtgaaatg cttggtcgca tgacgttttt
     8941 tatttaattc aagaaatttc ataatcgatt tccttttcta attctactgc ttccattcta
     9001 ccataatttg aaagagcttg cagggatttt tcatgattaa gtttttctat cacagacata
     9061 aaagatatat attggtttat caggaaagtt tctgataaac tagcaagtac tttacatttg
     9121 aatggagaca gtatgaaaaa atttagccta ttactagcta tcctaccatt tttggttgcc
     9181 tgtgggaatc aagctacacc caaagagact agcgctcaaa agacaatcgt ccttgctaca
     9241 gctggcgacg tgccaccatt tgactacgaa gataaaggca atctgacagg cttcgatatc
     9301 gaagttctaa aggcagtaga tgaaaaactc agcgactacg agattcaatt tcaaagaacc
     9361 gcctgggaaa gtatcttccc aggacttgat tctggtcact atcaggctgc ggccaataat
     9421 ttgagttaca caaaagagcg tgctgaaaaa tacctttact cgcttccgat ttccaacaat
     9481 cccctcgtcc ttgtcagcaa caagaaaaat cctttgactt ctcttgacca gatcgctggt
     9541 aaaacaacac aagaggatac cggaacttct aacgctcaat tcatcaataa ctggaatcag
     9601 aaacacactg ataatcccgc tacaattaat ttttctggtg aggatattgg taaacgaatc
     9661 ctagaccttg ctaacggaga gtttgatttc ctagtttttg acaaggtatc cgttcaaaag
     9721 attatcaagg accgtggttt agacctctca gtcgttgatt taccttctgc agatagcccc
     9781 agcaattata tcattttctc aagcgaccaa aaagagttta aagagcaatt tgataaagca
     9841 ctcaaagaac tctatcaaga cggaaccctt gaaaaactca gcaataccta tctaggtggt
     9901 tcttacctcc cagatcaatc tcagttacaa taacactctt cgaaaatctc ttcaaaccac
     9961 gtcagcttta tctgcaacct caaagctgtg ctttgagcaa cctacggcta gcttcctagt
    10021 ttgctctttg attttcattg agtataagat atactaaaag cgattggacc agccaatcgc
    10081 ttttttagtt tgcattggct attttaggaa actcttacaa aaaatcaaaa aaattcttca
    10141 catcctctac atacccatgc ttttctatta agctggctaa aagttccaga ttgtgcccct
    10201 gatagttatc gtcacgacgc agttcttcat acatttcaat aaagggaagc cccttggact
    10261 tggccaattc taactcagcc tcgtaatcat aagcaacttt acgaaattgg atattgacca
    10321 attccccatc ttcaacttct atcacggcat actgggcacg gtgatttttt aacgcctccc
    10381 aattaaaata gggcatgcca atcgaccctg gattgatgat ttgttgccct tgacttccat
    10441 aacgaagcaa ctgcttgtga acatgaccat aaactgccac gtccgtttcc gcatctagca
    10501 gttggtcaaa tttctctgta tcattctcaa ctagcaagtc accaccatag ttcttatttg
    10561 gcaaattatg agagatagaa aagcgcagtc cagcaatttc tttcttttcc agcaaaggca
    10621 agcttcgtag ccagacagtc gttgcaggat ccattcgctc catcaaaaac tgagtcattc
    10681 gcatgagctg gatttcctgt gggtcttcta agccatattg cccatctaaa gcctcaagga
    10741 cacaatcatt ccaattgcct cgaacacttg ccgtgatagg aagttccttt aacagagcga
    10801 ccaagtcatt tgcacctgaa ccagggagga aaatgtctcc catgagccaa tattcactgg
    10861 ctccttgagt tttagcatct gcaatcactg cttctagcgc cgtcgcattg ccatgaatat
    10921 ctgataaaat tgcgatttta tggtttattg tggtttcctt ccgtttggta atctactctt
    10981 tcttccgcca cttgaagcaa gatttctgtt tcctgcgggt tcaactttct ttgcacagcc
    11041 tctgctactg ctcttggtac tccaacttcg acaatctcat ccacactggc ttccttgatt
    11101 ttggtcagag acttgaagtg cctcatgaga ttttgcttgc gtttaggtcc cagaccatca
    11161 atcccatcca attgagatga gaaagaattt ttggagcgca gttggcggtg gaaggtaatg
    11221 gcaaagcggt ggacctcatc ttgaatgcgt tggaggagga aaaattcctg agaattgcga
    11281 gacaagtcca ccacttccag cggatctcca aagagcaatt catgggtttg gtgcttatca
    11341 ttcttttgca gacctgcaat tggaatatcc aagcccagtt cttcttggat gacttgctta
    11401 gcaatattga cttgaccttg ccccccatca atcacaatca aatctggagg agtcaaagcc
    11461 tcacgctgta ctcgaccata gcgtcttcga atgacttctc tcatactagc atagtcgtcc
    11521 ggcccaacaa cggtttttat cttgtactta cggtaatctt ttttacttgg cctgccgtta
    11581 acaaagacca ccatggctga aacaggactg gtccccatga tgttggagtt atcgaaggac
    11641 tcgatgcgaa ctggagtcgg aatttggagc aaacgtccta gattttcaat agctccttgg
    11701 gtcttttcga ctgatttttc tagcaggtta aatttctgct ccagactaac acgtgcattt
    11761 tttatggcta gattgaccag ctgttttttc tctccacgtt ggggtttgag aatcttggta
    11821 tccaccaaag ccttgacagc ttcttcgtca atatcctgcg gaatcagtac ctcattggga
    11881 actaggtgag atttttcttg atagaattgt cccacatagg tcaagaaatc ctcatctgga
    11941 tcattgaagt aggggaagag attgacatcg cgctcgatga gcttgccctg acgaacaaag
    12001 aaaacctgca cacacatcca gcccttatcc acatagtagc caaagacatc gcgattttgg
    12061 agatccttag ccatgacccg ttgcttggtt cgaagcgttc caatagcctg aatcaggtca
    12121 cggtattccg ccgcacgttc aaactccata ctttgtgctg ctactgccat tttactcttg
    12181 agatcatcga tgattttgtc atcctgacct ttcagaaaat cagacacctc ctgagccata
    12241 gacttgaaat aagcctcatc cttcttacag atggtgtggg ccatacactg gccgatatgg
    12301 taataaaaac agaccttaga gggcgggttg gtacattttc gaaaagggaa aatcctatct
    12361 agaagtcgct tgatttcatt ggctgccccc acatctggat aagggccaaa ataaagacca
    12421 ccgtcctttt tgacctgacg agtaatgata agccgtggat agcgctcatt ggtgattttg
    12481 atgaaaggat aggacttgtc atccttgagc atgatattgt acttgggctt attttccttg
    12541 atcaagttga tttccagcag aagcgcctca atattggact cagtgacgat aaattcaaaa
    12601 tccacaattt cagacaccag agcctctgtc ttggtatcat gaattccacg aaaataggac
    12661 cgtactcgat tacgcagatt tttagccttt cctacataga taatggtgcc atttttatcc
    12721 ttatgaatgt agcaaccagg gctggtcggc aagagctcta gttttgattt aatcaagttg
    12781 ttcatagttc cattatagca aaaaaagtag ggaaagatag taccctactc actagtctca
    12841 tatatttttc ggaggtttgc aacatcctct tcctggatac cataaaagga acctgctggt
    12901 tgcatgacag acttctcatc tgtatggtcc aagccaatcg catgacctaa ctcatgttct
    12961 gccgtatgga caaggcgttc ataggagtag ccatagtatg gattggacaa ataataatga
    13021 ttcaaccgca ccgttacgga caagaattgc cctgttaaga gattagtttg actttccgcc
    13081 tctcctgcca caggagtgcc tccgtcgttc atctccgtag ccgtaatatc cgccttacta
    13141 gactcagtca cgagttcaaa gttaaaagca ccagtttgat tccagttctg gattgcttct
    13201 aaataagcct cttgaaagcg tgaatccatc tgcggatcca ggtaaatacg agcagaggcc
    13261 tgtgcccacc ttccttcaga atgctggtga ctatctgtct ggcttaactt aggcagttgc
    13321 cctgttttct cccattggtc aatactttgt tgacccattt ttactgaccg ttctgcttgc
    13381 tttagcgctc cttgaaaatc cccgttcaga taccagagaa gtccgaaagc aagcacacat
    13441 aagagcacaa ctatccaaac cagacgccaa aacaaacgcc acacaaaaga aaagaaagcc
    13501 cctatcaaac gaaaaagcca gcgcatgtct ctccaccttt ctagcaaata agaattattt
    13561 tactaaaaga agctaaaaga aatctaaata ctgacttttt catcttaacg tcctactatt
    13621 tttttgagta actggatagc tttgtatttt aaaggcgatg gatggtaacg gaaagtacct
    13681 gctttgcgta caatatagcc attaaaattc tgtttaaaac gcaaaacacc atcacttcca
    13741 tcaaaaatcc cttgaatgcc taggaagttg tatttaggta ttccacgttt tatgctttcc
    13801 aacataacat atttttgaag cagtgcaggg gcatagaact tattaaactc agtgtaggaa
    13861 ccactaaaga gataagtcgt ttcctgaggc atataaacaa ataaactccc agctaaaaca
    13921 atatcttctt ctccatattt ttcaatcaag tctcgcgctt ctgcttttcg aacttcaaac
    13981 gtttcaaatt gactagaata ttctctcagt tgattttgtt ttttctcaga atgaggattt
    14041 ttactcaaat caagtcgcaa cttgtccaag ttttcttcta gtttactttg ttcaccttgc
    14101 aatttgctca tatactccga aaaattcaag cttgctatga gaaactccgc ttgttctcca
    14161 aaagtatcat aaaaatgctc ataatattct aaacttttat cactatattc tctacgttca
    14221 gaggtttctt ttgttatatt cttaaaaatc gatagttctt cacgttttaa ctttttcaac
    14281 cgaatgccaa aggtttcagc ctttttcacc aagggtttac cctttttgct aaaactttta
    14341 agcaaactct tttcagttaa ttcagttaaa tctttatagt ataaccaatc tggttctcca
    14401 cctgggtaac ctgttgttaa gccatcaaat tgataaccta aatcagtcaa accttgaata
    14461 atactttttt tctcagcatc tattggatta ccttggctat caaaagtttg ataagtttca
    14521 taaggtttta caagcaactc taatacacca ttttgcttgg catattcttt taactctgca
    14581 taaaaaactg gaagagcatc ttgttgggta taaatcggcc ccgaattgag ttccatatgc
    14641 agaccaccca gcatgggcaa gctataaacc agagctgcaa cttgaatttc tccttcttgt
    14701 ttcaaagcaa gataaacaat tcgagcccct cttttttcta gcaaatcccc catctggaca
    14761 gattgcataa aggaacgaga agaaacctga tcagaataag tctgaaactc ttctttcgtg
    14821 agtgttgtta gtgccatata cttactttct atgttttttt cttaatgttt tacggaaatc
    14881 aagagcaagt cttaacagag gatagagagg atgagtgggc attgtaaatt cacccaagta
    14941 ttcttcaatc gttggattaa atttttcctt aaaatgataa agtccaccat tgagagagtt
    15001 ttcaacacca cctaaatttt gccagaccat acctcgctca aaggcatagc gagccgtttc
    15061 ataccatgtt aaaattggtg cattgtaacg tttaaaatca tcatccatac cagcatatat
    15121 attgacagag gtagtaccaa attccaaact caaagtagcc gctaaaggaa ctctcgcttg
    15181 acctacatct atatattcct gcaagaaggt caattcctct aacaaacgtt ctttttcctt
    15241 cttctgcgct tctacttttg aagttcgagt cgactcagta aaggtctctt ccaaggctct
    15301 atttttcgct aactgttctt ctaactcttg cgaacgttta gaaacatcca aggtggccaa
    15361 ggtgatatag gccttgtcct taaaattatc taacaatttt ttataatagg cttcattcct
    15421 caaatgaatc tcttttcgct tctcagtttt tttcatcaac tccgaaaatg aatctaatag
    15481 ttccagtcca ccatattgaa tctcaagccc tttgtttcgt gctgttcgaa tagcctgttt
    15541 tgttgacttg gaaagtttat cttcttcaaa attttccttg tatattttcg cctgaatacg
    15601 aggttgaatg gtgtctccca tttcctccgt ttttcctgac caccttactc ccatttgttg
    15661 caaactatca ataatagcca gattttcagg aaattctgtc ttttcctgat tgattaaact
    15721 ttgagatagg caaatacttg ggtcaaaagt cacaaaaacc gctctcttac tgcgagcata
    15781 ggacttaata gactgaatgg caaaattcaa gagttcttta tccccataat ccaatatagg
    15841 tcctcttggg atgtaaaaca ttttatagcc tagcggaaga gttctaatca aaatactagc
    15901 tgtcgccagt aatttttctt ccctgtaaac accaaacttc tcatgttgcc aattagactt
    15961 aacttcctcc caagcactac tttgtaatac attggctaat tcatgttctt tgacaaactg
    16021 atcatattct aatgtgggaa tgccaatttg ataacggtac atttcctact ctctttcctc
    16081 cagtatttta ttactattct actactttcg tttgaaaagt ctagtgaaaa catattttat
    16141 tttagttcta ctccccctat gctgaagttc aaactaagtc agtctctctt ctaatttgaa
    16201 atcaattggt agggttgtta aaaaaacttc caattgtttt tcccagtagt accactcgtg
    16261 agttccagcg ctatggctat aggtcacatc aaaacctagt tttttgagat ttttcactgc
    16321 gagattattg gcttcgtaca agaaatcctg ttcgccacac cacgcccaaa gtttggtctt
    16381 tttatccgat tttttagcca gactttcaag agaataggga ctagttgtcc agtctctaat
    16441 ctctccaaaa acacctctcc agtaggctgg acttcccaga ttttgacttt caggagaaaa
    16501 gttttgaaag ctgagggcac ctgaaaaact agctgcatga gaaaaacgat ttgtcgcaag
    16561 agccagtttg aagcagccgt aacctcccat agaaagacca gcgataaagg tcttttcacg
    16621 cttgctagtc atattaggga agaagcgttt cagaacctgt ggcaattcct ctgctagagc
    16681 cgtgtagtag tcaaaaccat actgggtatc ggtgtaccaa ccattgctgg tattgggcat
    16741 aacaacgatg agattagttc ctcgaagcaa gcgttctaca ttggtccgct taagccaact
    16801 attatgattt ccagacatcc cgtgcaaaag gtacaagacg ggaatatctt cacactctgg
    16861 ttcttccact cgattggcat cagggtagag gacattcacc ccccactcca tatccaatac
    16921 ttgtgagtaa tactcgattt tcattactgc catgattttc tccttctatt tttctataag
    16981 aaaaagccga aactcgactt tctctctgtt tatttcaacg attattttgc ttccatgact
    17041 actggtaaaa tagctggacg acgcttggtt tggtcaaaga gatacttggt caaattatct
    17101 cgaaccttcc ctttaagatc tgcccagtca aagtcatctc cttgaagata atcttctacc
    17161 gtttggttaa tcaattctga actttcacgg agaatatcgc gactcttctt gagataaaca
    17221 aatccgcgcg tatgaacacg agccctagcc acaattttct tctcacgacg gttgactgta
    17281 atagccacga tgaaaattcc atcctctgac aagatcttac ggtcacgaag aacaacattt
    17341 ccaacatcac caatggcatt cccatcaatc aagatatctc ctgctgaaac cgatccagct
    17401 ggaacaaagt ctccattctc gtaagccatg gtcgtcccct ttttaggaat gaagatgtgt
    17461 tctggcaaca tcccaactgc catggcagcc ttagcgtgag catccaactc acgatactcc
    17521 ccttggacag ggaagaggta cttaggttgc aaaagattga tcatcagctg caaatcacgc
    17581 acatttccgt gccctgatac atgtaaactt tgggtaatca atttgacaac cccacctgcc
    17641 tgataaatca tattttccac acgcgcaaca aaggcttctt tagcaataga cggagccgta
    17701 gcaatataga ccaggtcccc atccttgatt tctacataac gatggcgacc aatcgacatc
    17761 ttacgaagtc cattgatagg ttcacccata cgacctgtct caagaataat caactcatgg
    17821 tcttcaaagc gagacatatc tttaggctta atcaagagaa tttcgttggc taaagacaac
    17881 ttcttaagac gaatcgctgt gcggacgata ttttcaatat caaatcctgt caagacgata
    17941 cgtcgacctg ttttatccgc agcgtcaaaa atctgctgaa tacgagaaag attactggaa
    18001 acagctgcaa cgatgatacg accttcccag tcagcaatag tttgggtaat ttcatcccta
    18061 acttcacttt cactagccac ctgaatattg ctgtctgcat tggccgaatc actgaggaga
    18121 gccaggacgc cgtcacgacc aatctctgcc aaacgagcaa aatcagttgc ataagattca
    18181 ctagccgttt ggtcaaattt gaagtcacct gtataaacga tgcttccttc cgatgtcttc
    18241 aagacaattc ccagactctc tggaacggag taagtcgtag ggaagaagga aaccactgtc
    18301 ccaccaaaat caatctccgt attctcatca atgacatgga aatcattaaa tttcttaacg
    18361 gcatcatttc ctttgacaaa gagctttgcc aactcaatgg tcaactcaga cccaaataca
    18421 ggaactttag cctctgccaa gagataaggt agagcaccaa tggcatccgc atgcccgtgg
    18481 gtcaagaaaa ccccagcaat acggtcgcta ttttcaaaaa ggtaatccat gtttggaatc
    18541 accacatcga cccctaattg ttcattttca ggatatttta accctacatt caaaacaaaa
    18601 atggactctc caatttcagc aatgtacata ttttttccat tctcacgcac accaccaagt
    18661 gttgttaaac tgatattact catttttcct ccaaaatctt agtttatctt gattataggg
    18721 caattctacc ctcttattct aaaagttctg atactttcag ccgaatcttt tcctaccatt
    18781 ataccatttt tttgatgatt ttcaaaatga aggtctgttt tcaaaaaaga gttgatttcc
    18841 caactctttc ctaacttcta ctctttttcc atcaaaaaat cataaatttc ttgcaccgta
    18901 cccagcgcca tcatttcttg gtctttaacg gtttgtttga gattttggta aatttgactt
    18961 tctcctatct cccgctttgg cgcctcttta ttgactgtca tcacaccatc ttttatcgtc
    19021 acaaagccta gttcttcaaa tacttgaatc atcttgacca gcaagatttg ttgaatatta
    19081 agatatgcag ccaaatcttt cagcttgtag cgaatatcaa actctgggaa ctggtaaata
    19141 gtcttgtaca atttagcaaa ctgatctcta gtcccataac ctgtcagata ataggtcttg
    19201 tcaatatcat ttttgaaata gacagcagag aaatgctgtt cctgaaaaat ggtcttcagc
    19261 tgagtaatat cctctggaat gttttttacg acaacagctt cactagccgc aagatttggc
    19321 agttctccag gaaaatccaa gactggaaca ccttctggca agactgcatt ttttccacga
    19381 atgttaaaaa gttgaacacc ttccactcgc gcatccacca tcatcaactg gagggcagtt
    19441 tggccattcc attggttgac agacaattta accgctaact ctagattctt ggtttgagaa
    19501 aactctgtcg cccatctgcc ttgaccaaag gctaccactt caaaactcgc ctcacccttg
    19561 gaaattttca gctttagatg ggcattacct gcccccatag tacgagcact ttcgacctga
    19621 aaattcttga tataaaaaat aggtttctga ttatccattc caaaaggagc taaacgttca
    19681 aaacttttga ccgtttccaa gctaagtgcc tccaaatcca actcttcatc taggtttaac
    19741 ttattcttgc caccagcatc tgcacctttt tcacgaacat aatcttccaa aacctgagat
    19801 aaatctgaga gttgctcaac ttccagcgtc atacccgctg cacctgcatg acctccaaag
    19861 gcgatgaaga ggtctcgatg gggatccaga gcttcaaaaa tatcgaccgc ttccacacta
    19921 cgagcactgc ccttggcacg accgtcttct atattaagaa caatgactgt ctgtcccaat
    19981 tcttccaata aacgaccagc cacgattcct agaaccccag gattccagcc ttccttggcc
    20041 aagacctgaa ccttcttctc aggatccacc atggtcttgg cttcttcata gatagactga
    20101 acgatttcct tgcgctcttc gtttttctgg tgaatcataa gggcaatctc atgcgcttcc
    20161 tcatcatcaa atccagtcaa caaatcaatg gcaggattgg gatcatccaa gcggcccaag
    20221 gcattcaaac gaggagcaat ctggaaacca accgtttctt ctgttacttc gttggcagca
    20281 atcccagcca tgtccagcat ttcttgcaga ccaatgcgct gggtatgacc caacatttcc
    20341 agaccatatt gaactaagat acgattttca tccgtcagac tcaccatatc tgcaatagtt
    20401 ccaatagcga ccaaatcaag caattccact tgcacttctt ctaacagggc acaagccaac
    20461 ttgaaagcaa ctccacaacc agccaaatat ttaaaaggat aatccgcatc tggatgttca
    20521 ggatggacaa tagcataagc atctggcagg gtttcaggca tggaatgatg gtctgtcaca
    20581 atgacatcta ctcccataga ctgagccaat gcaatagcct catgaccagc aaccccattg
    20641 tccaccgtca caatcaagga aattccttct tgctcgataa agtatttata aacactagca
    20701 ttagggccat agccatcggt aaaacgattt ggcaggtaaa ctcggcactc agcaccaagt
    20761 tgttccaaac tttccttcac aatagaagcc gaagtcatgc catccgcatc atagtctcca
    20821 taaacgagaa tattttcccc ttcttcaata gcctgacgaa tccgctccac tgccttgtcc
    20881 atatcatgga gcagataagc atcatgtaag tcctccaagg aaggttctaa aaacttcttc
    20941 agactttctt ggtcctgaat ccctctctca aacaataacc gagccacctc aggacccagt
    21001 ccagccttct tggctatctt tgtaaaatcc gcatcttcta cctgcagggc aaactgccat
    21061 tcataagtag gtgttatcaa aaagacatcc actctttcta agaattctat tgcttcatta
    21121 taacatgatt tagacgtttt ctctatccag attgcttttt tataacattt tatagtatat
    21181 tgaaactaga atagtacacc tctacttcta aaatattgtt agaaatcgat ttgactgtcc
    21241 cgatcgattt gtcctgttct tatttcacta tagtgaattt tttcagatat ggtttgacaa
    21301 gaacattctt tcattgtaga atcgtgttat aaaatataac acaaaggaga tctctcatgg
    21361 ctttagtaga atttaaaaac gtcgaaaaat attacggaga ctaccacgca ctccgcaaca
    21421 tcaatctccg ttttgaaaaa ggacaagttg ttgtcctgct tggaccttct ggctctggga
    21481 agtccactct tatccgtacg atcaatggtt tagaggctgt tgacaaagga agtctcctag
    21541 tcaatgggca ccaagttgct ggtgccagcc agaaagattt ggtacctctt cgcaaggaag
    21601 tcggcatggt ttttcaacat tttaaccttt atccacacaa aacggtgtta gaaaacgtga
    21661 cacttgcgcc cattaaagtt ctaggaattg ataaaaaaga agctgaaaaa accgcccaaa
    21721 aatatctgga atttgtaaat atgtgagaca agaaagattc ctatcccgcc atgctatctg
    21781 gtggacaaaa acagcggatc gccatcgctc gtggtcttgc tatgcatccg gaactcctcc
    21841 tctttgatga accaacatct gctcttgatc ctgagactat cggagatgtt ctagcagtta
    21901 tgcagaaact ggcgcatgat gggatgaaca tgatcatcgt tacccacgaa atgggctttg
    21961 ctcgagaggt tgcggaccgc attatcttta tggccgacgg agaagtttta gtagatacga
    22021 cagatgtcga taactttttt gacaatccaa gcgaacctcg tgcccaacaa ttcctcagca
    22081 aaattatcaa ccacgaaagt gacaaagtca aataaggagg cgcctatgaa aaaaaattct
    22141 ttttatcagc attattgatt agccttttcg gccttgctgc tgccaaacca gtccaggctg
    22201 atactagtat cgcagacatt caaaaaagag gcgaactggt tgtcggtgtc aaacaagacg
    22261 ttcccaattt tggttacaaa gatcccaaga ccggtactta ttctggtatc gaaaccgact
    22321 tggccaagat ggtagctgat gaactcaagg tcaagattcg ctatgtgccg gttacagcac
    22381 aaacccgcgg cccccttcta gacaatgaac aggtcgatat ggatatcgcg acctttacca
    22441 tcacggacga acgcaaaaaa ctctacaact ttaccagtcc ctactacaca gacgcttctg
    22501 gatttttggt caataaatct gccaaaatca aaaagattga ggacctaaac ggcaaaacca
    22561 tcggagtcgc ccaaggttct atcacccaac gcctaattac tgaactgggt aaaaagaaag
    22621 gtctgaagtt taaattcgtc gaacttggtt cctacccaga attgattact tcccttcacg
    22681 ctcatcgtat cgatgccttt tccgttgacc gctctattct gtctggctac actagtaaac
    22741 gcacagaact actagatgat agtttcaagc catctgacta cggtattgtt accaagaaat
    22801 caaatacaga gctcaacgac tatcttgata acttggttac taaatggagc aaggatggta
    22861 gtttgcagaa actttatgac cgttacaagc tcaaaccatc tagccatact gcagattaag
    22921 gaggacaccc catgatagat ttatcatctt ggacagccta ttttcaggat tttggacaat
    22981 ttttcaatgg tttcctcttt actcttgccc tagcgattgg atcctttatc ctcgccatgg
    23041 tcttaggaat cttctttggt gccatgtcaa ccagtaaacg tccaatttta cgcattttag
    23101 ctcacatctt tgtggaattt taccaaaaca ctcccctctt ggtgcagttt gttattgttt
    23161 tttatggcct acctcttatc agtgaccata ctatcatgat tccaatttat tggacagctg
    23221 ttctctgcat gggactctat cacggcgctt atatcgctga ggttattcgt tcagggattc
    23281 agtctattcc tagtggtcag atggaggccg ccttgtcgca aggttttacc tatatcagtg
    23341 ccatgcgctt gattatcttg cctcagacct tccgcatcat tctccctcca ttgaccaacc
    23401 aaattgttaa cctcatcaag aacacctcta ctgtagctat catctctgga gtagacttga
    23461 tgtttgtgac taagtcttgg tcggctctca acggaaacta tatcccagcc tttttaggtg
    23521 ctgctcttct ctactttgct ctatgcttcc ctgttgccca gtttggtcgc aagatggagc
    23581 aagccaacaa aaaagcctat tcactttagg aggttactat ggaatctatt ttagaagttt
    23641 taaccccaga taacctagtc tttatcttta aaggatttgg cttgaccctc tatatttctc
    23701 tgattgccat catcctctct actatcatcg gtacggtgct agctgtcatg agaaatggca
    23761 aaaatcctgt cttacgcatt atttccagta tttatatcga gtttgtgcgc aacgttccca
    23821 accttctctg gatttttact atctttttgg tgttcaaaat gaaatccaca ccagcaggta
    23881 ttacagcctt tactctcttt acatcagcag ccttggctga gattattcga ggcggtctca
    23941 ataccgtaga caagggacag tacgaagcag gaatgtcaca aggcttcacc tcagcccaaa
    24001 tcctctacta catcattctc ccacaagcca tccgcaaaat gctaccagcc atcatttctc
    24061 agtttgttac cgtgattaag gataccagtc tcctctactc tgttatcgcc ctacaagaac
    24121 tctttggagc cagccaaatt ctcatgggcc gttatttcga accagagcag gtcttcagtc
    24181 tttgcatcct gattgccctc atctacttca gctttaacct agcaatttct agcctgtctc
    24241 atatgctagc caaacgttgg caacaagctg cagaataata ctcttcgaaa atctcttcaa
    24301 accacgtcag cttcaccttg ccgtatatat gttactgact tcgtcagttc tatccacaac
    24361 ctcaaaacgg tgttttgagc agcctgcggc tagcttccta gtttgctctt tgattttcat
    24421 taagtataaa cagccactct ccagttcaat tggagagttt tttgatgaga gtagatattt
    24481 atagacctca atttgacacc atacattttc tatgataaaa tgtaacaaat tcatttacaa
    24541 caaatcgaaa tctaaatgaa atggaatttt ctatgaaatc agtcaaagga ctactcttta
    24601 tcatagctag ttttatcttg actcttttga cttggatgaa cacttctccc caattcatga
    24661 ttccaggact agctttaaca agcctatctc tgacttttat cctagccact cgtctcccac
    24721 tactagaaag ctggtttcac ggtttggaga aggtctacac cgtccacaaa ttcacagcct
    24781 ttctctccat catcctacta atctttcata actttagtat gggcggtttg tggggctctc
    24841 gcttagctgc tcagtttggt aatcttgcca tctatatctt tgccagcatc atccttgtcg
    24901 cctatttagg caaatacatc caatacgaag cttggcgatg gattcaccgc ctggtttacc
    24961 tagcctatat tttaggactc tttcacatct acatgataat gggcaatcgt ctccttacat
    25021 ttaatcttct aagttttctt gttggtagct atgccctttt aggcttacta gctggttttt
    25081 atatcatttt tctatatcaa aagatttcct tcccctatct aggaaaaatt acccatctca
    25141 aacgcttaaa tcacgatact agagaaattc aaatccatct tagcagacct ttcaactatc
    25201 aatcaggaca atttgccttt ctaaagattt tccaagaagg ctttgaaagt gctccgcatc
    25261 ccttttctat ctcaggaggt catggtcaaa ctctttactt tactgttaaa aattcaggcg
    25321 accataccaa taatatctat gataatcttc aagccggcag caaagtaacc ctagacagag
    25381 cttacggaca catgatcata gaagaaggac gagaaaatca ggtttggatt gctggaggta
    25441 ttgggatcac ccccttcatc tcttacatcc gtgaacatcc tattttagat aaacaggttc
    25501 acttctacta tagcttccgt ggagatgaaa atgcagtcta cctagattta ctccgtaact
    25561 atgctcagaa aaatcctaat tttgaactcc atctaatcga cagtacgaaa gacggctatc
    25621 ttaattttga acaaaaagaa gtgcccgaac atgcaaccgt ctatatgtgt ggtcctattt
    25681 ctatgatgaa ggcacttgcc aaacagatta agaaacaaaa tccaaaaaca gagcttattt
    25741 acgaaggatt caagttcaaa taagtaatca tactctcctg ggatttccca ggagagtttt
    25801 ttatagccaa atgaaataaa accggaacga tggaattttg gcattcaaat caattgataa
    25861 gaatatttta tagtggagtg aaataggata cgaacaaatt gattaggaaa atcaaatgaa
    25921 tttatagaaa tcttttagca gttatgttgt cctattctag tttcaaaacg ctatagaagc
    25981 agcattgtgc tagtctagat tcagtttact atactaaaac gagtagcttg aaatcaaaaa
    26041 acccaccctc acaggcaggt tttatctgta ttattcagct agattatgct ttaccttctg
    26101 aaccgaatac gtcgatacgt tcttcaaccg atgcttggat agcttttaca ccgtcagcca
    26161 agaatttacg tgggtcgaag agttttttct tgtcgtattc tgcttcgttt gcttcgtagt
    26221 cacgagcaaa tttacgagtt gcgttagcga atgcgatttg gcattctgtg ttaacgttaa
    26281 ctttggcaac accaagtttg atagctgctt ggatttgctc atcaggaata cctgatccac
    26341 cgtgcaatac gattgggaat cctggaagag cttctgtcaa tttttgcaag tggtcaaggt
    26401 caagaccttc ccagtttact gggtaagggc cgtggatgtt accgatacca gctgccaaga
    26461 agtcgatacc agtttcaacc attgctttag cgtcttcgat tggagccaat tcacctttac
    26521 cgatgattcc gtcttcttca ccaccgatag taccaacttc agcttctact gagatacctt
    26581 tagcgtgtgc tttttcaaca acttctttag ccaatttaag gttttcttca actggaaggt
    26641 gtgaaccgtc aaacatgatt gaagtataac caacttcgat acactcaagt gcatcttcgt
    26701 agtgaccgtg gtcaaggtgg atagctactg gtacagtgat acccattgat tcaacaaggt
    26761 tagcgatcaa gttgcgagca actttgtaac cacccatgta tttagcagca cccattgaag
    26821 tttggatcaa aactggagct tttttagctt ctgctgcgcg caagatagct tgagtccact
    26881 caaggttgtt tgtgttaaat ccaccaactg cataaccgtt gtcacgggct gcttggacaa
    26941 atttttctgc tgaaacgatt gccattttat caggcctcct gtatattttt atgggtcatc
    27001 ccatttacat tgttcatttt atcacttttt gccaaaaaaa tctagttttt cccgcagttt
    27061 cgattgattt tcttctaact ccatctatgt aaaccctttc tctccctagt cttggacgac
    27121 ttttggaaaa tctataaaga aggttaaacg attctcctcc atctcgaaac gataagctaa
    27181 tttttcatgt tctaatagac tcttaaccac aaagagcccc ataccagacc ccttgacctt
    27241 gcgactggca ttgtcagaaa aagactgggc tagtttttct tgttcctctg agctacagct
    27301 attttcgata aaaagttctc cttctctttc tccaattcga actaagccac ctggaacaga
    27361 gtgcttaatg gcattgctga tgagattaga aagaatcaac ttcataactg atgggtttag
    27421 ataagcctgc tgatgggtca aactattgtc tatctggagc tctctttcct tggctagcaa
    27481 ggcataatct ttgaccagat tttgcgtcat ctggaggagg tcaattgttt ccctatcatc
    27541 tcgcaattcc tgcacagaag agagggaaag tatctgcaga acatggtgat tgagttcatc
    27601 cacaatcccc aaggcaactc ccagatactg gtctctatcc ttataacgac cgatattctc
    27661 tctcatattt tcgattagga ttttcaaact agccagcggt gttttcaatt catgagaagc
    27721 tcctcgtagg aattcgacct tcatcttctc cagctggaga atggcttcat tcttttcatg
    27781 caagtccgca ataacagtca agagatgctg gtagaggcta ttgatttgtt ccttgagatt
    27841 acctatctca tccttagaat ccacgcgcaa tcgcacttgg gaatccaggt ccatcatccg
    27901 acgggtcacc cgcttgattt ccaagatcgg tgcaacaata gtccgagcgt agatgtaggc
    27961 caccaagagg gaaatcagaa aggaggccag caaggtatag ggaagaaact ggagactgat
    28021 ttgctccgct tccttttgta aatccatgga agctagaaac tggagaatca tagtagcacc
    28081 gtcttgcgtt ttcacctcgc gctcctcaat aaagagagag gttgtctggc ggtctgtgtc
    28141 cagaggaaga ctgtccttga cttctaactt gtcctcggtc atctcacctt tgacggtccc
    28201 cttgatatca ctagtctggg aatacaagtc taacacttgc tcgatactct gcctatcttt
    28261 cccttctagg gactgggcaa tggctgttgc cttttgacca atggtttcct gacgatgact
    28321 cagataagtc gaaggaaaaa gaaaataaat agctaaatga aggcagataa ccagaacact
    28381 aaatatcgag aaggtataga taaatatctt tgcaaataaa cctgttcgtt tcattttcgc
    28441 tccaatttat aaccaacatt gcgcacagtg aggatacaat ccaagtctag ctttttccgc
    28501 aattccttga tataaacatc aataacacgg tcaaagggaa cctcatctgt cgctttccag
    28561 acggcatcga taatctgaga tcgagtcaag gcccggcctt catttttcac tagatagtcc
    28621 agaatttcca actctttggc attgataggc acttcttgac ctgcgatgct tgcactgtag
    28681 ctttcaaagt ccaccttggt atccttgtaa gaaaagattc gtcctgtatc gtagtagcgc
    28741 ttgaaaatcg cgtccaccct cacttttaaa agggagaggg agaaaggttt ttccagatag
    28801 ccatctgcca aagaggcaaa ggcactcatc ttgtattcct catcttgaaa agctgtcaac
    28861 atcaagacag gaacctgact ggttttacga atctcagcta ggacttctaa gccgttgagc
    28921 ttgggcatct ggatatccag taaaaccagg gccacctcat agctagaaaa ttgctccaga
    28981 gcttcctgac cgtccgctgc ctcaatagtt tcatagccac aatccgtcaa ataatcactg
    29041 accccctcac ggatcatctc ttcatcttct acaattaaaa ttttcatact ttaactgctc
    29101 tctatttttt atttttctta gaataaatac ctactctatt ttctattata gtctcttgct
    29161 ggccttttgt ctgcaagcaa ctgaccacta gataaaacgt tgtgaaattc ctttctcata
    29221 aattccataa ctttagtata ttatatttaa gcactaaagt acaaagaaag caactgaaag
    29281 caatgatttt caccactgct ttcggattta ttttgaattg ttaaatagcc attcctatcc
    29341 actattcttg aatagaaaca caagatgcaa tctttattct agactcattt tttcaaattt
    29401 attcaccatc cagcaagagc tcttttggtt gttttctaag gagattgctt gaagcaagcg
    29461 ccataacgag aaccactaga accaaggcaa ggacaaaaat gatgataaag tctgatgtct
    29521 gaatggaaat gtctaggctc gacaaggtct tgctaaagcc atctacttct gcaccaccac
    29581 caaggttaga ggcttgagcc gccttactag cctgtttggc aacacctgaa gtcacattgg
    29641 caaggacagt gtttccaatt gcacgggcag tgtaattagc taggaagtaa gcagaaacta
    29701 gagcagggat agcaatcaag atagattcgg tgatgaattg acccaagata cttgcctgct
    29761 tgaggccgat agagaggaga attcccactt ccttgcgacg ggcgttgatc caaaggctga
    29821 gcaagagggc aaggaggaga actgagaagc tcaagctacc ccagaagagg aggttggcca
    29881 tcttgtacat accagagata gattgctcaa gagctgggta gttagaggag ctcttgacga
    29941 gtgtgtagct cttccagttg ataccactga tgccattcaa ctctttcata acatcatcca
    30001 agttcttgtc tgctgttaca aagaaggttg cgtccccata aatggctgtg tcttctgtgt
    30061 atccataaag ttttgcagca gtgtgaatgt ctgtaatagc tgtgttttcg taaagttctt
    30121 gtgagtaggt tactgctgac ttattatgac catcaaagag tcccttgatt gtcacttcaa
    30181 ctgtttcctt ggctcctttt tcattatctg catcgtagat attagagtcc agtttaacct
    30241 tgtcccctac tttccagccg tgtttggctg ccaagtcctt gtgcaagagg attttatcct
    30301 tgtcgtcgtt ggttaagtgc tctccttcga ctagtttata agaaccagag acaaacttgt
    30361 cttctttaga ggagtcattg acacctgtaa tcatcaagct acttccaaaa cgcttggcac
    30421 gatcagcagt gagattcttc ttggtttctg gcgtttcaat caggtcatat ccagtcaaat
    30481 ctccgatagc gttgatacgt ttgacataag actcgatggc cttgttttcg gtgatttttt
    30541 tgatgtcttc acccttgata ttcccagcac cacgaggcgt tccttggttg acgcgacgat
    30601 tgatttgcat ggagaagcta ttagtgatat ttttaaaggt ctcctgagaa gccttggcag
    30661 tagctccctt gattgacaag ccgaccaaac tcaagctcgc catgaggaga ataatcagga
    30721 agatgacaat cgatttgaaa aacttccttg taacataggc aaatgcgttg tgtaacatag
    30781 attccctttc tagattttgt tttaatcatt ctatcaaaat aagctcaaat tatttactag
    30841 tattgcgcgt ttcagtcagt ttcttatcct ttaattcaag tgtaatatct gacgcttgtg
    30901 ccacttcttt actgtgagtt acgacaatca cacatttacc tgttttctgg gcaagtgatt
    30961 tgagtagttc gacaatatct ccagcagttt taggatccag atttcctgtt ggctcatcag
    31021 ctagaataac tggagcttct gagaccaaac tgcgagcaat ggcaacacgt tgctgttgac
    31081 cacctgataa ctggagaaca ttccgcttga tctggctttc atccaaacca agctcaagaa
    31141 gtgtatcctt gcttgccttt ttgttgacca agcggatatt ttccagcgga gaaagataat
    31201 ctatcaagtt ataattttga aagaccaggg aaatatggtg catgcgatgg taagaatagc
    31261 ccttcttacg aatatcctct ccttgaaaaa ggatagaacc ttcaacagga ctatctagac
    31321 cagcaagtag ggacaagagt gtggattttc ctgctcctga ctccccaata atactgtaaa
    31381 attttccagg ttcaaaatta taatcgatct gatataggac tgcttcagca gtattcttat
    31441 aacggtaggt aacatcttgt aattgtaata aagtcatgat ttctccttct taactaatag
    31501 atgataaaat ttctttcggt gattttctaa ataagaatag gaaacaaagg gctacagata
    31561 agcaactaag cagaactaga aaaacatagg attctgcaaa agataagatg ctagttgata
    31621 aactgcttgc tttggctagt gtatcttgta agcttgcctg atctccactt gctagtagag
    31681 tttggagtag ataacttgtg attccgtttc ctgcaacaaa tgctggaagc aaagctccaa
    31741 gagataccaa aactacctct aaacagaatt gtaggaagat cgagctcttg ccttttccaa
    31801 gtgcaagtaa aatccccact tcatagaccc gttctctcaa ccagagagac aaaaccagaa
    31861 ttaaggctcc agctcctgct atcaacatcc cataaaggaa gatggtcaag aaggtttgga
    31921 aagttgcaac tgagtctttg atttgttcaa aagccttgtt ttccttttcg acttggtagc
    31981 cttgattttc caaggccaag ttttctacct gcttcatgag tccgtccatt tccttaggat
    32041 tttctacata gaagcgtgct gcactgactt gagcttcact attgcccaaa agggtttggc
    32101 tactttcata gtctgtaaag acttgatttt cactgaagtc agaagacaag cctgtgaatt
    32161 tctcttgttt tttaccagaa aagatgccga taatctcaaa ctctactgtt tgtccttttc
    32221 cagattcaga ctgaccagca tccaagccaa tcttgtcatg aagtgaaaga ccgttcttct
    32281 tagccaattc ttcgtggata aggattttct tggaatcccc tttttgaagg tgtcgccctt
    32341 cttttagatt gaaagccgaa ctggtaaagg ttacatcctt ggatgaatcc tcaagagccg
    32401 ttaagctaac caagttattg tctgcagctg ataaatcatc acgctccacg ctctgctcac
    32461 cagtcactgc ttccttgtct tttagttttg cgaccgtctc aagttcagga gagacatttt
    32521 ccagcccctt aatcttgctt acagatgcta ggtctgacaa cttgaatgtc tgaccattct
    32581 ctatcttctt aatagaaaaa gatgtattga gtgatttata aagattgctt tctactgttt
    32641 tgttggactt catcagagtc aaacaggctg aaattccggc caataagacc aataaaatca
    32701 gaaataaaat aaaacttctc agtcgctttc tgctgacata agcccaagat ctttggattg
    32761 gattcatttg tcacctccat atttgtaaga ctattataaa acccaaatat gaaatgttta
    32821 tgaaatacga aaaaaaatat cgagtagggg ataatctcta gcccctctca caccaccgta
    32881 cgtgccgttc ggcatacggc ggttcaacta acttttaacg catgtcgttc aaggtaataa
    32941 tccaaacacg aaacctgttc acgtttttca aggactggtt ttgatatagc acgtttaagt
    33001 accgacttct gagctactat agtagattga aactagaata gtacatctct tcttc
//
LOCUS       DAVBCB010000020        31002 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVBCB010000020 DAVBCB010000000
VERSION     DAVBCB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 31002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31002
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.020"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(298..516)
                     /locus_tag="VQU28_000896"
     CDS             complement(298..516)
                     /locus_tag="VQU28_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476272.1"
                     /translation="MVLNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(762..1040)
                     /locus_tag="VQU28_000897"
     CDS             complement(762..1040)
                     /locus_tag="VQU28_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476273.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEMLELACWFRVIIVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYSIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQU28_000898"
     CDS             complement(1254..2885)
                     /gene="rlmD"
                     /locus_tag="VQU28_000898"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2476274.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSLHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWCTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSQKNASLNKITNAHYVCDTAENAMKKWLKEGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(2959..3933)
                     /locus_tag="VQU28_000899"
     CDS             complement(2959..3933)
                     /locus_tag="VQU28_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HET2476275.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQU28_000900"
     CDS             complement(4007..4621)
                     /gene="pmp23"
                     /locus_tag="VQU28_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HET2476276.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     KDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(4621..5595)
                     /locus_tag="VQU28_000901"
     CDS             complement(4621..5595)
                     /locus_tag="VQU28_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HET2476277.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQKENGTYSILIKNIEDIQSK"
     gene            complement(5603..6859)
                     /locus_tag="VQU28_000902"
     CDS             complement(5603..6859)
                     /locus_tag="VQU28_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HET2476278.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(6921..7352)
                     /locus_tag="VQU28_000903"
     CDS             complement(6921..7352)
                     /locus_tag="VQU28_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476279.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(7354..7956)
                     /locus_tag="VQU28_000904"
     CDS             complement(7354..7956)
                     /locus_tag="VQU28_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HET2476280.1"
                     /translation="MMDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRR
                     PRDKALNLNIASFEDILHFSKNQPTYLQKLVETFLPGPLTIILEANDRVPYWVNSDLA
                     TIGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFL
                     TGQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQU28_000905"
     CDS             complement(7940..8779)
                     /gene="prmC"
                     /locus_tag="VQU28_000905"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HET2476281.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKKVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQU28_000906"
     CDS             complement(8779..9858)
                     /gene="prfA"
                     /locus_tag="VQU28_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HET2476282.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(9855..10445)
                     /locus_tag="VQU28_000907"
     CDS             complement(9855..10445)
                     /locus_tag="VQU28_000907"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HET2476283.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRGLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            10568..10750
                     /locus_tag="VQU28_000908"
     CDS             10568..10750
                     /locus_tag="VQU28_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HET2476284.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            10887..12260
                     /gene="mnmE"
                     /locus_tag="VQU28_000909"
     CDS             10887..12260
                     /gene="mnmE"
                     /locus_tag="VQU28_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HET2476285.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     NKVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEANLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLIEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQU28_000910"
     CDS             complement(12307..13242)
                     /gene="dapA"
                     /locus_tag="VQU28_000910"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HET2476286.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKTIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(13298..14374)
                     /locus_tag="VQU28_000911"
     CDS             complement(13298..14374)
                     /locus_tag="VQU28_000911"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HET2476287.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(14716..14788)
                     /locus_tag="VQU28_000912"
     tRNA            complement(14716..14788)
                     /locus_tag="VQU28_000912"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14753..14755),aa:Thr,seq:ggt)
     gene            complement(14888..15397)
                     /locus_tag="VQU28_000913"
     CDS             complement(14888..15397)
                     /locus_tag="VQU28_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HET2476288.1"
                     /translation="MKKLTIRDIADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(15395..15491)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            15520..15951
                     /locus_tag="VQU28_000914"
     CDS             15520..15951
                     /locus_tag="VQU28_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HET2476289.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            16033..16410
                     /gene="mscL"
                     /locus_tag="VQU28_000915"
     CDS             16033..16410
                     /gene="mscL"
                     /locus_tag="VQU28_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HET2476290.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQU28_000916"
     CDS             complement(16452..17546)
                     /gene="hemH"
                     /locus_tag="VQU28_000916"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HET2476291.1"
                     /translation="MKKAILMMTFGSPEEISFEGVAEFFTNIRRGVRPQDHEIQTLYD
                     NYIRIGGTPLQRITREEVNLVKERLGEEYGIYFANKFSRPFIPDVIKQMETDGVEECI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEVLLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQU28_000917"
                     /pseudo
     CDS             complement(17624..18456)
                     /gene="srtG2"
                     /locus_tag="VQU28_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218039.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG2"
     gene            complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQU28_000918"
     CDS             complement(18534..19532)
                     /gene="srtG1"
                     /locus_tag="VQU28_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000851047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system class B sortase SrtG1"
                     /protein_id="HET2476292.1"
                     /translation="MKTKREKPKKSLSRRLVLAVDGVINHLLLIFAALIFLFGFYALW
                     DSNQVYSLASSSEYEAYRPVTTQQDELASFSGFSKLQELNPEVLGWINVYGTNIDYPL
                     VQAKDNEKYLNKDSKGEFAATGAIFLDARNNPKFEDFNTIIYGHHVENGVMFGDVAKF
                     ADQEFFDQHRYGSIYYNGVEKGLEIFEMLEVDAYDFNIYDPGIQGEDRQQAYLDHLLS
                     VAMHKRDISLSPSDRIILLSTCFLDVTNGRHIVVAKITDTVPKNTFHTKKSKPFPYSV
                     FDDSSLGRFLSSIPLWIWYLILFVLFLLLIFLLLVLYLILRRRRESKKNARSRPFY"
     gene            complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQU28_000919"
     CDS             complement(19550..20746)
                     /gene="pitB"
                     /locus_tag="VQU28_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus major pilin PitB"
                     /protein_id="HET2476293.1"
                     /translation="MKKTFFKKLFTASIAAITALSVFRGVPTFADDNSAITKANGENN
                     AVVKINKTLNIAEGITTPTATFTFKFTEKTGQSSNGAPYQTGVAIPDRNVEYNKNDHP
                     TADKIQKATEDIFSGVAYGHAGEYVYDVAEAKTGWQAITKNGKTIDAMRYDKRTYEMH
                     VIVKNKVNGGVYISSVYFKENNKSNAPKVEPSEQGVYNLFDNTYTKDASKEPNPDDPS
                     QVDPNAKALTITKKVDGASGDKTRDFQFHIKIQLPSTNKTAETPVTNIIVKHGSKSEV
                     LAVVTPADTVEYNFTLKDGETFTVEQLPAGSKYTVTETGVAGYTDSSIYTTNGAEQTS
                     QGQKNVDFTLTDILIGEKKNDNKVTNKIDDVTPTGLLIDNLPFILMIGLGLAGFVVLS
                     KKRREA"
     gene            complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQU28_000920"
     CDS             complement(20791..21342)
                     /gene="sipA"
                     /locus_tag="VQU28_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PI-2 pilus system signal peptidase SipA"
                     /protein_id="HET2476294.1"
                     /translation="MLLKKKHKKPVTQVNRDKSPPSVWGDILYLVSKLLMVGFVLAIL
                     YFFVFGLLRYNDDGMKPALKDGDLVVYYRLDKRYSIGDLLVYSYKGKERVARVIATEG
                     STIDINENGLIINGSPQQEQDIYKETLLYKEGATFPMKVPAGQLFVLGDNRTTAVDSR
                     AFGTIPIQDTQGKVVTVIRRRGF"
     gene            complement(21342..23297)
                     /locus_tag="VQU28_000921"
     CDS             complement(21342..23297)
                     /locus_tag="VQU28_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HET2476295.1"
                     /translation="MDAGIGTGTNINAGLTEAQRLLQSARAGAKKVVILLSDGEANMY
                     YESNSGRTIYNYYSNPNVGRMIDTPYWFTSGLERGMLNISSLIAPKIDGFYSIKFRYY
                     IGSNDSITSLKGYISGYNSGIPNEIFSANNENDLQQKFKEITDKILPLGVHHVTISDV
                     LSKYVQLLPGDASHLRVVKIKDGNEQELNDNQVTIETKKNEQGLVEVTAKFNPSYTLE
                     DDAKYVLKFTVTSSQEAFDAIAGDKTLTSDDAEEADATKLYSNKGAKVAYSYGIGTSR
                     TKIKDYSEKPTFKPSDPLTVPVEIEWKGVDGKSNPSANRPPSVELNLNQKKDGSIKDS
                     YRKVTSPVQTNSFTENTSFAKVAKGYDYELKAPDAPGYTVEVQKTGTKEKPSFKVIYR
                     QLPSLTVKKILEGEQSPNKSFTINVTFSDKDGKPINGKFGNTTVTNGKAQISLKNSQE
                     TALSYLPRDTHYKVEEVENSRTGYHVTYEKQEGTLSEDVQTIVTNHRLPTLSVTKKVT
                     GAFANLLQSFKITINVKDAQNKPLNGSYSAIVNNQKTTLQFTNGKATVDLKKDKTIKI
                     LDLPLNARYSIEEEASSSRGYQVSYDKKEGTLDANKSATVTNNKNSVPETGIDFLSST
                     LVLGVVLPLGGIFFIILLGHLVVNRRK"
     gene            complement(23427..23930)
                     /locus_tag="VQU28_000922"
     CDS             complement(23427..23930)
                     /locus_tag="VQU28_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HET2476296.1"
                     /translation="MNVQYDFKKIQYFTSSLVIFLAILFLCAPINSLRADSITEPQTT
                     LHKTITPISGQKDQYELSLDITSKLGTETQSEPLDVVLVADLSGSMEERDVWSYSSRR
                     YISRIEALKHTLKGVNGRQGLIDTILSNSQNRLSIVGFAGKIDNQYNDRYYNEYYLSY
                     QYGTWPN"
     gene            24289..25512
                     /gene="pepT"
                     /locus_tag="VQU28_000923"
     CDS             24289..25512
                     /gene="pepT"
                     /locus_tag="VQU28_000923"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HET2476297.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPRQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25548..26177)
                     /locus_tag="VQU28_000924"
     CDS             complement(25548..26177)
                     /locus_tag="VQU28_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476298.1"
                     /translation="MNKANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDSQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSANSKDKTALLESTKAILVLNHQIQSKVSASEETSPARNAEANSDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            26142..26363
                     /locus_tag="VQU28_000925"
     CDS             26142..26363
                     /locus_tag="VQU28_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476299.1"
                     /translation="MDLFQELICLVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(26634..26982)
                     /locus_tag="VQU28_000926"
                     /pseudo
     CDS             complement(26634..26982)
                     /locus_tag="VQU28_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27087..27647)
                     /locus_tag="VQU28_000927"
                     /pseudo
     CDS             complement(27087..27647)
                     /locus_tag="VQU28_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669441.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQU28_000928"
     CDS             complement(27873..30992)
                     /gene="phtE"
                     /locus_tag="VQU28_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HET2476300.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQISQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         8835 a         6360 c         5686 g        10121 t
ORIGIN      
        1 aaaaggatct agtttttatt tttagttaaa ttatgtctat atttttcatt aactcatcaa
       61 taaaaatttg tttgctaata tctctttctt ctgattttgt ttcaaggatt attgctacat
      121 cattcatatt tttaggtaaa taccaaccat ctggcttatc tgaatatccc ttgaagccta
      181 attgattaaa agtagttatt tgaccagtac cttgattgat gttttcttca acttcattaa
      241 agcctatact tctcttgcat aatccctaac ttgatcttct gtccttaaag tcattttcta
      301 ttcctcgtca ctaaaattat cttcaaaact aataatatca ttaggagtgc aagataaagc
      361 cttacatatt ctttctaaat tcttaaatgt aattggttta tcctttccca tttgtgccat
      421 aacatttgtt gtaagtcctg ccatagctat tacatctgtt ttctttagtc ctttttttgc
      481 taactgtatc cataatggtt tatagttaag taccataata tccctctttt tttcttcatt
      541 aaatttattt aattatagca taaatattga tttcattcaa tcttacattg atttagtgat
      601 ttgttttaga gaatttgttg tgttattccg tccttatttt gccataaatt gttttaagtg
      661 ggacctctta tccttaccat ttttacctcc taattttgag tataaaaaag acgatagagt
      721 ttttaatttc tatcgtctga gatttacata ttaaattttc atcacaaaat aagactcttt
      781 aaagaatctt taaattcatt ggtatgctgc ttacaatatc cattattaaa atggatacta
      841 taagcagcac accaactatt tattttttga gttttaaact ttggtagaag tcttgaactc
      901 cttatattta cataactatt tgcctctata actattatga cacgaaacca acaagcaagt
      961 tcaagcatct ccatatcttt attcaaatga ggactaagat attcacaaac tttatcataa
     1021 acctctttat cttttttcat aaaaggggca tacaacttag caaatttatc ctaaaatttt
     1081 ttgttgcctt tatcaatcat ttcaacgctc ctttaaatat aaaaataatc ctatcaatct
     1141 ttatattgag cctattttct aattgatacc ttgcaagaaa ggaataatag ctgttcctat
     1201 accaagtagg aatgtaataa atgcagaaag tcatatactg tcattctttt ctattaaatc
     1261 attttgaagt gtctcaaagc atccatgatg gcttcttctt tttcaggtgt acactgtgga
     1321 ataatttgtt tatccttttt agacttgttg taatgttctc gtaattctat tccacatttc
     1381 ttttttatct gtgcaatata taatgtcgaa acttttaatt caaatttatt ccaaacatat
     1441 tctttgattt gagcatatgt tgctttgctc tccgcacttg tcaaatccat ctcatccagc
     1501 tcaatttcaa cacttatgtg cttatcgaca tcgagtttgg acaaaagtgc taccgtctcg
     1561 acgtgatgcg tttgaggaaa tagatccacc ggctggactt tcttcaattc atatcccaat
     1621 tcttggtaga gtttgatatc acgcgccatg gttgcgacat tgcaggagat ataggcgatg
     1681 cgatcggctc ctgtttgggc gcttgctttg ataaagcttt ctgtcaagcc cttgcgtgga
     1741 ggatcaacca agataacggt tggttgaata ccttccttga gccatttctt catggcattt
     1801 tcagccgtgt cacagacata gtgggcatta gtaatcttgt tcaaagaagc attcttctgg
     1861 ctattctcaa ccgcttctgg aatcagttca acaccgtaga cttctttgac atgcttggcg
     1921 actgataaac caatggttcc aataccagaa taagcatcaa taaccacgtc atcttttttt
     1981 aactctgcaa agtcaatggc tgtttgatag agtttctccg ccatttcagt attgacttgg
     2041 taaaaggctg ggccagcgat ttggaagtca tttcccaaca tctggtccgt aatatagtct
     2101 tgaccataaa gagtgcacca ctccttacca aaaatcgcat tggtattctg gtcgttgata
     2161 ttttgcatga cagacacaat ctctgggaac tgcttgataa cttgttcaat caattggtca
     2221 acacgaaaaa cttttggacg agttgtcacc aaaacgacca tgatttgtcc tgaatagtga
     2281 ccacgacgca ccacaagatt ccgaatcaat ccagactgtt ccttttcgtc ataaggtttt
     2341 aaatcaaaac gacggagcag gtctcgaaga gctactacga cttggtcaat gacaggatcc
     2401 tggataaaga aatcttcaag gggcatgagg ttgtgcgaat tcttacggaa aaatcctgtt
     2461 tccaagacac cattcactcg acgaacgggc acctgcgcct tattgcgata cttgactgga
     2521 tgttccatac caagcgtttc agcaacttct acatctgcaa ttccagcaat cttgtagaga
     2581 ctgtccttga cttgcttggt tttaaacttg agctgttctg gataagaaag gtgtcctaaa
     2641 tccgcgattc ctgaacgcag gtaagccaaa tctagatctt gattacggtg tagtgactgg
     2701 acaaggtatt tttcaacttt tccaaagcca atctttttat tgaccttgag gacacgcatg
     2761 agaatttttt cactcggtaa agcattctct acaaaaaaga ccaaaccatc taccttggca
     2821 actcctgccc cttcatgggt caaatcaaca atttcaactt ctacaatatc atttttcttt
     2881 aacattcttt tctctttcta ttggccgaca aaaaagacgg caacgttacg attaccatcc
     2941 attataccat gaaatttctt aagaaccaaa actcttgaag aagttgacaa gggcttgcca
     3001 gagggagctt aagaagttac caccgtcctc tagcgcctta tccgcatcaa agttaaggtt
     3061 gatattttta aaactgtcgc cagcttgtga tacgatgctt tgtttaaggt catttagggt
     3121 tttagtgaaa tctgcattgc tgaggatatc actctttgag agattcaagg caaaattgat
     3181 gatgatattg atctggtttc ctgttatgac ctgatcaagt ttgtaatttt ttaaggtatc
     3241 ttcaacaatc ttgcggacat cttcttctgt cagatttccc ttgctttctt tagctttggc
     3301 gagtcctgac ttgatatcag ctagggcaac gtttaattta ttagcatcat agcctgattt
     3361 gtccttgttt tcagcattga tatctgacaa agcctttagc tcttcttgag ccaaatcttt
     3421 attggcttgc ggcaccttgg ctccattagc ttctagcgaa tagtagatcc cagccaaagc
     3481 actctcacct gtaactggaa taggggctgc tacagtgatt ttggcatgtt ccatacccag
     3541 cgttactgct gcgtttcggt acatatcctg agtcacctta gtgatatttt ctggtgtttc
     3601 aatcttgacc tcaagtggcg atttgtcacc tagcttttga atcttggctg atgaatacaa
     3661 ctgtaagcta gagtcattgg ccacattcat gattttagaa taaacatcag gtgtcatggt
     3721 cttgagttct ttggtatctg ttgaggcatt gtagcccagt tttttaagag tttgattttt
     3781 ttggtcttca gatagggagg aacctaggac atattcaggt tggacatagg tttcatcgat
     3841 aaccttttga acatctgttg ctgcatggac gctattcata gctgttactg cccacaagac
     3901 cgcagcactg gtcagaaaga gtttctttct catagggaat ttcctccttt acttttctag
     3961 agtaatatat ctatcttaaa gaaaacttat aacaaaaaca cctggtctag ccagatgttg
     4021 aaaagagagt gaaacatttg atgatgtaaa ggttaagtcg tacctgtcta gaataataat
     4081 agtttcctcc atttacatag agttcagcac cgtgaaaaat ggaaatgggg tgaatataac
     4141 tataagtctt tccagtcgta ttaccaagca agggggcaac agtctcacga gagtactgtt
     4201 tggctagagc caaggtattt tccttgccat tttgggcgat aaaatcgata taggcaggtc
     4261 caaaattata ggcttgaaca gctgtccaga tatctacccc cttcttctgc gccagataga
     4321 gattgcctgt cagagtttga atgccttgcc gaatgctaga ggcattatca ttgatggtgt
     4381 tggttgaacc acttgcagac tcactagact gcataacatc gccttctttt ccttttgttt
     4441 cagtataaat catagcaagc acaagctctt cgtttgctgg ggtgtctttt tcactcaaga
     4501 tttctcgcac catgggttga taggtcatga cttgtttgac atcttgatga acgcggtaag
     4561 ctttatagcc agcaaaaagg aagactgcta gtacaagcac tcttcgaatt cgtttaaaca
     4621 ttatttactt tggatatcct cgatattttt gattaagata gagtaggttc cattttcctt
     4681 ttggataaac tcaacagact cggcgtcttg atagacgtta ttgggaacga tgagctcaat
     4741 tccatttgat aaggagagtt tttggttttc aaatttcttt aattggcgac tggcatcaat
     4801 ttcatcaaat tgaacaggtt ctggtacggc ttctttgact tggtcaataa agctcaaacg
     4861 agccgtcaga ttgttgtcaa aaaggtcatt agccaatttc tcaggtgaca attcattgct
     4921 ttcttctagg ttgttgaaaa tcgctgattt gaccttggat tgaaattgaa aatcatctgt
     4981 gttaaaagat tcagcaattc tctgggctgt tttttccagt tccttgatag attttttagg
     5041 agaaatctta ggagcgacag caagaagatt atctgaaaaa tagttcaaaa aagtcccgtt
     5101 gtacttgatt cgtttttcaa tcaggtgata cttgcgactc tgaagattga ccaccaaggc
     5161 ctcgtcagca cccgttccaa atccaggcag gttattctga gtcagcttga ttggattatc
     5221 aacttctcct ccgaggtggg tcaaggtctc ccgcagggca attcgcaaga aagcgaaatg
     5281 ttctacacct tctttagaaa attgaacaaa aatcaagtca ttggtcttga gattttcaga
     5341 aatgctaaac tcctctttcc agagattagc cagcgttact gatgtctcca acaaatcgtc
     5401 tgtaatatga ttgaagaagg gattttcttc ttcgaaaatc ccagtcttgg cttcatctga
     5461 atacacatgt tcaatttttt tacgtaggta ttcttcgatt tttggagtaa tattgagaaa
     5521 cttatctgct aagaacagct cggtatcatc cggactgaac tggtgaataa tggctttctt
     5581 aatataaatg tccataaaag ttttattcct cgtataatgg gaaggcatct gtcaattctt
     5641 tgactgcact tctcacttct tctaatacag cctcattttc tgaattctta agggttttaa
     5701 tgatgagttc agccactttg cgactttctt cttcaccaaa tccacgtgca gtaatagctg
     5761 ctgctccgat acgaatccca cttgtcttga atggtgacaa ggtttcgtaa gggattgaat
     5821 ttttatttag ggtaatattg acttcatcca gcaagttttg agcaactttt ccgttttcta
     5881 caaccttagt cacatcaaca aggaagagat ggttttcagt tccacctgaa ataatacgga
     5941 aatcagggtc ttgcaagaag acatctgcca tagccttgct gttcttaatt acattggcag
     6001 catattcctt gaaggctgga tccaaaactt ctttgaagga aactgcctta gccgccacaa
     6061 catgctctaa aggaccgccc tgaatacctg ggaaaatagc tgaattgatt tttttagcaa
     6121 gttcttcgtc attggtcaaa atcaaaccac cacgaggtcc acgaagggtt ttgtgggtcg
     6181 ttgttgtagt gatatgagcg tatggcactg ggcttgggtg aagaccagct gcaaccaagc
     6241 cagcgatgtg ggccatatca accataagct tcgcaccgac agcatctgcg atttcacgga
     6301 attttgaaaa gtcaataatt tgagaatagg ctgaagcacc agctacaatc agttttggtt
     6361 ttacttcttg ggcttgtttc aagatagcat caaagtctaa gagttccgtt tcaggatcaa
     6421 cactataaga aacaaagttg taggtttgac cagagaagct aacaggagcc ccatgagtca
     6481 aatgaccacc tgatgccaaa tccattccca taaccgtatc acctggctca atcaaggaca
     6541 tgtaagccgc acagttggcc tgacttcctg agtgaggttg gacattggca aatttagcac
     6601 cgaaaatttc ttttgcgcgt tcaatagcaa gagtctctac aacgtctact acatcagttc
     6661 caccataata acggcgtcct gggtaaccct cggcatattt atttgtcaag atagaccctt
     6721 gagctgccat aacagccttg gaaactacgt tttccgaagc aattaactcg atattatttt
     6781 gttggcgttc ttcttctttg gcaatagcat tccagagatc agcatcatat gctttaaaat
     6841 catctttgtc aaaaatcata ggtcttctcc tttattgtgt gactagtcca ttagtttgat
     6901 tttacaataa gaaaatcaaa ctaaaagatg cgaataaacc gtttctgcat tttatcacaa
     6961 gtatagccaa ctttttcata aaatgcatga gcacccagac gatgattggc agaatttaag
     7021 cggataaacc cataaccaca tcttttggct tcttgttcca acccttgtag taaactttta
     7081 ccgatacctt gaccttgcgc ttgaggtgaa actgctaaag ctaagatatt aaatcctgct
     7141 ttggaataga gtgattcgta aacttcagcg tggacatatc caagtaagac atgattagct
     7201 gcatcctcat agccaagtag gaaatgatgg gaatcctgag acagtctagc tagttggcta
     7261 gccgtttcct ctggactaaa agtataaccc aaagcctctt ggttgatgtc acatatcgct
     7321 ttcacatctg tttcttgcaa atctcttagc atttcaagcc tcctcaaaag aaatctctgg
     7381 caaccgagca agaatatctt ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc
     7441 tccagacaaa tccacaatag ttgaatcctg tccagttaga aaagcatcgt cttccagacc
     7501 cagaacctct tggtcaaaat ccttcagaat ttgttcaaag gttacaccac ttgcctgacc
     7561 tgagatattg gcagacggcc caatcaaggg acctgtctct cgaattaaat ccagtgtgat
     7621 agggtgactg ggcatccgaa atccaatagt tgcaaggtca gaatttaccc aatagggaac
     7681 tcggtcattg gcttcgagaa taatggtcaa gggacctggc aaaaaggtct ctacaagttt
     7741 ttgtagataa gttggctgat tcttagaaaa gtgcaagata tcctcgaaag aggcgatatt
     7801 gagattgagt gccttatctc taggacgacg tttgagttgg taaacatggt caactgcttt
     7861 ttcatctaag gccttggcaa aaagaccata aacagtctct gtaggtagaa cgacagctcc
     7921 accattttcc aactcttgtc taatcctgtc catcatcaac cacaaccatc ctattttgac
     7981 caaattggtc tttgagtgtt cgaacttttt tttcaggaag atgttttcta aaaagttcgg
     8041 gaacactttg accttgcttg tatccaattt caaggtaaat cttaccacca tccttgagat
     8101 agtcttttgc atcttccgca attctacagt aaatagctag gccatcctcg tctgcaaaga
     8161 gagctagatg aggctccgaa tgcaaaacat tcaagcctac ctctgactca tcttcacgag
     8221 agatataggg tggattggat acaattatat catatttttc agaaatttct gcaaaacaat
     8281 cagatttttt aaaaaatata ttaagatttt gatcgctagc attctctgat gcaagctcta
     8341 aggcctcttg agaaacatct gctgctgtca ctgaccaatc tggtctattt tttgctagag
     8401 cgagagcaat agctccacta cctgttccaa tatccagaac tgagagattc gtctcaagat
     8461 tttcagttag aataagctcc actaactcct ctgtttctgg acgaggaatc aaaacccgtt
     8521 catccacctt taaatgcatt ccataaaaat ctgcctgtcc aatgatgtac tgagctggct
     8581 tgtgagctgc tagttgctgg taaatatctt ctacaaattg tttttcttcc tctgttgtca
     8641 cctcctgctg gagggcaaaa ataaagtctg taaaagatag atttttcaga ctacgataga
     8701 caaaagagag gctttccgct tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa
     8761 ataattgagc taatttcatt atttgtttaa ttcttctagt ttttgtgttt ggtcataaag
     8821 caccaaggca tccacaactt cgtccaattt accagacaaa atcgtatcta gtttttggag
     8881 ggtcaagccg atacggtggt ctgtgacacg gttttgaggg aagttataag ttcggatccg
     8941 ttctgaacgg tcaccagtac cgattgtcga cttacgctca gcgtcttgtt catcctgagc
     9001 aatctgagca aagtggtcag cgacgcgtgc acggataatc ttcatagcct tctcacggtt
     9061 cttctgctgg gtacgttctt cctgcatctc aaccttgata ttggttggca agtgaacgat
     9121 acgaacggca gtcgcaacct tattgacgtt ctgtccacca gcacctgatg cgtggtaaat
     9181 atcaacacga agatcttttg gatcgatatc gtattctact tcttcgactt caggcatgac
     9241 aagaactgtg gcagttgagg tatgaacacg accttggctt tctgtcacgg ggacacgctg
     9301 cacacggtgg gcacctgatt cgtacttaag cttagagtat acagactgac ctgaaaccat
     9361 agcaaccact tctttaaacc caccgacacc attcatagag gcttccatga cttcaaagcg
     9421 ccagccttgg gcttccgcat acttttggta catagttagc aaatctccag cgaaaagtgc
     9481 cgcttcgtct ccaccagctg ctccacggat ttcaaggatg atattcttgt catcgtttgg
     9541 atcctttgga aggagcaaaa ttttcagttt ttcttcatat tcttcttttt cagccttggc
     9601 atctttgagt tcttgcttgg ccatttcttc caagtccgca tctccgcctg attccttaat
     9661 catctcttcg gcatcgacga tattttgaag gacttgttta tactcacggt aggctattac
     9721 ggtatcacga ttggaagctt cttcttttga aagctccata aaacgcttgg tgtctgaaac
     9781 gacatcaggg tcactcagca attctcctag ttcttcataa cggtcttcta cagcttgtag
     9841 ttgatcatag atgttcattt ttcttcattc tccttattga tttcaggggc aaaataatgt
     9901 ttacggcaaa ccgagatata ggtttcatta ccaccaattt ggatctgttc tccatcgtaa
     9961 acgggcagtc catcctgtgt tcgcaacacc atggtcgcct ttttcttgca atactgacag
    10021 atggtcttga tttcgtcaat cttgtctgct aaaagcaaga gatatttgga accttcgaac
    10081 aattcattgc gaaagtcatt tttcaagcca aaagccatga cgggtatgtc taactcgtcc
    10141 acaacacgag ctaggtcata aacatggtgg cgtttgagaa actgggcttc atcgaccaac
    10201 acacagtaag gtttttctgg taggcctcgg atatagccaa agatatccgt tgtttcctca
    10261 atcgcaaggg cagggcgttt catgccaatt cgactcgaca catagccaac accgtcacgc
    10321 gtatccagag ctgaagtcat aatcacaaca ccttttcctt gctcctcata gttataggcc
    10381 accttgagaa tctcgattgt ctttccagag ttcatggtcc cataacgata atacaactgt
    10441 gccatgtttc ttgcttcacg tccatttcta aatttttgct acattctagt atatcataat
    10501 tttcttaagc tttaaacggc aaaatgtggt aaaatggaag aaatcaaaac tagtggagga
    10561 agctattatg ccatttgtac gcatcgattt atttgaagga cgcacgctcg agcaaaagaa
    10621 agctcttgct aaggaagtaa cggaagcagt tgtccgcaac actggagccc ctcaatctgc
    10681 tgtccatgtc atcatcaacg acatgccaga aggaacttac ttcccacaag gggaaatgcg
    10741 tactaaataa gctagcttaa gcagaattgc ttaggctttt tcaatctcca agtagcattc
    10801 attgaagaaa tatcctaaat ttgttacaat ttgaaaagaa acttggagaa tttccaagaa
    10861 aagagctatt aattaaagga aacattatga ttacacgtga atttgatacc atcgctgcta
    10921 tctctactcc actaggtgaa ggggctattg gtattgtccg cctgagcgga acagacagtt
    10981 ttgctattgc gcaaaagatt tttaaaggaa aagacttgaa caaggttgcc agccacactc
    11041 tcaactacgg tcacattatt gatcctctga ctggtaaagt catggacgag gttatggttg
    11101 gggctatgaa gtctccaaag accttcactc gtgaggatat tatcgagatt aacacccacg
    11161 gtggtattgc ggtgaccaat gagattctcc agctagctat ccgtgaagga gctcggttgg
    11221 cagaacctgg tgaatttacc aaacgtgcct ttttaaacgg tcgcgtagat ttgacacagg
    11281 ccgaggcggt gatggatatc atccgcgcca agacagacaa ggccatgaac atcgcggtca
    11341 aacaacttga tggttccctt tctgatctta tcaataatat ccgtcaagaa atccttaaca
    11401 cacttgccca agttgaggtc aatatcgact atcctgagta tgacgatgtt gaggaagcca
    11461 ctactgctgt tgtccgagag aagacaatgg agtttgagca attactaacc aagctcctta
    11521 ggacagcacg tcgtggtaaa atccttcgtg aaggaatttc aacggctatc attggacgtc
    11581 ccaacgttgg gaaatcaagc cttctcaaca acctcttgcg tgaggacaag gctatcgtaa
    11641 ccgatatcgc tgggacaaca cgagatgtca tcgaagagta cgtcaacatc aatggtgtac
    11701 ctctcaaatt gattgataca gctggtattc gtgaaacgga tgatatagtg gaacaaatcg
    11761 gtgttgagcg ttcgaaaaaa gccctcaagg aagccaactt ggttctacta gtgctaaatg
    11821 ccagtgaacc actgactgcg caagacagac aacttcttga aattagccaa gataccaatc
    11881 gcattattct acttaataaa accgacctgc cagaaacgat tgaaacttcg aaactacctg
    11941 aagacgttat ccgtatttca gtccttaaaa accaaaacat cgacaagatt gaagagcgaa
    12001 tcaacaacct cttctttgaa aatgctggct tgattgagca agatgctact tacttgtcaa
    12061 acgcccgtca catttccctg attgaaaaag cagttgaaag cctacaagcc gttaatcaag
    12121 gtcttgagct ggggatgcca gttgatttgc ttcaagttga cttgactcgt acttgggaaa
    12181 tcctcggaga aatcactggg gatgctgctc cagatgaact catcacccaa ctctttagcc
    12241 aattctgttt aggaaaataa gaaaaatcca tgatccttca ttcggtcatg gattttattg
    12301 tctttattag taatctggtc ttaagacccc tgttacagtt gccttagttg cttcgtagtc
    12361 gccatctacg acaaccttga taatgcgttt ggcatcttct tctggtgctg gaacaagagg
    12421 tagacgagtg ggtccagctt caaatcccat atagttaaga attgccttaa ctggagcagg
    12481 acttggataa gagaagagag cattaacctt aggaatgaat ttacgctgaa ttgctgcggc
    12541 tttcttcata tcgctttctg caatggcagt aaacatctcg tgcatttcat ccccatttgt
    12601 atgagaggca acagaaataa ccccatccgc cccaaggttc atggcatgga aagcatctcc
    12661 atcctcacct gtataaatca agaactcttc aggcttgtgc tcaatcaagt aagccatatt
    12721 agccaagcta gtacattctt tgacaccgat aatatttgga tggtcagcca agcgaagcat
    12781 ggtttctgga gtcaattcga caactacacg ccctggaatg ttatagataa taattggtag
    12841 gtcagaagca tctgcaatag tcttaaagtg ctgatacatc ccttcttgag aaggtttgtt
    12901 gtagtaagga acaatagcaa gcccagctgc gaaaccacca aattccgcta cttctttgac
    12961 aaactcaata gagtcacgcg tatcattagt acctacaccc gcaatcaaag gaacgcgtcc
    13021 attgacaacc ttttgtacag ccgcaaacaa ctccaactcc tcatcgtggg tcaaagttgg
    13081 actctcagca gtcgttcctg cgagaagaat tccatccgta tgatgggcca ataaatgctc
    13141 aatcaaggct ggaatagcat caaagttaat agaaccatcc tcatgaaagg gggtaataaa
    13201 ggctgtaatg attttacatt cttttaaatc ttgataagac atgaaaacac ctctctattt
    13261 caaagaagga gttcatctga actcctaaac gatatgacta ttttaattca aatttcaatt
    13321 cggctgttgg acgaaccaat ccacgttcat gaagagtttc agcaatctga actgagttcc
    13381 aagcagcacc tttgagaagg ttatctgaaa caacccacat gtgaattcct ttttctgcat
    13441 ccaagtcttt acggatacga ccaacaaagg tatcacgcga accaactgca ttgatagctt
    13501 gaggatagat ttgatgagct acatcatctt caagaacagc acctgggaag gctgcgatag
    13561 ctgcttttac ttcttcgatt ggagccactt cttttgtttc gatataaaca gactcagagt
    13621 gagctgacaa gactggaata cgcacacatg ttgcagatac tgcaatgcta tcatcttcca
    13681 taattttctt agtttccttg gtcatcttca tctcttcgta cgtgtaatca ttatcagtga
    13741 aaacatcaat ttgtggaaga gcgttaaagg cgataggata atgtttcttg tcaccacctg
    13801 aaggcaaaat ttccgcatgc aaatcacatg gtttcacacc atcattcaag acttcacgaa
    13861 gttcacgttg tgtctcaaga attgctccca taccagcacc tgaaacggct tgataagttg
    13921 aaacaatgat acggtccaag ccccattttt ggcgaaccgg ctcaagagcc accatcattt
    13981 ggattgttga acaattaggg caggcaatga ttccgttgtg ggcatcaagt gcatgagcat
    14041 tgacctctgg aacaaccaaa ggaacatctg gattttgacg gaaataagat gtattatcca
    14101 ctactaccac gccagctttt actgcgtatg gtgcatactt agctgatgta gaactacctg
    14161 ctgaaaagag agcaatatca actccttcaa aagctgtttc agtcgtttct tcaattgtaa
    14221 tatcttgatc tttaaatttc aatgacttac ctgctgaacg tgcagaagca aggtaacgga
    14281 ttttatcgat tggaagtgtt gattcttcca acatttttat catctgagca ccgacagcac
    14341 ctgtcgcgcc gactacagca actgtatatc ccataaataa cctctttcgg aattttctaa
    14401 aaatttctat aatagaagta ttatactact ttttccatga attgcaaagc tttttcatca
    14461 ttttttggaa aataaaaatc ccagtcgcta gactagggac taagaggcat cttcatgtga
    14521 tgagcaggtt cacacaactc atcaaggtcc gctcctgcgt tatgacctcc ttatgctcaa
    14581 tagtagtccg aagactacct atcgactcat cgcatattct atattactag agatacttga
    14641 ttttgtcaat aagaaatttt ttcgatcgag tataacaaaa aactctcgta ttcaagagtt
    14701 tccctataca tatcatgcta attgccggga tcgaaccggc gacctcatcc ttaccatgga
    14761 tgcgctctgc caactgagct aaatcagctt acttaagaag tatactatat tcagaaaaac
    14821 ttgtcaagtt tccttagaaa ttttccatag gaaaaagcca ggtaagaact acctagctta
    14881 tcttcttcta tttacttaaa tcaattcgac gaccaatttt accaataata atcgccccaa
    14941 taatcaatga agcaaattca ccgattcctg ttgtaaacca agtaaggaag aatggtaatt
    15001 ctgctacaat atttaactct gcagcgatag taaacatgga aattgaaaag aggattgaaa
    15061 agaagaaatg atcttttcga atcaatccgt taaagaggta atccttgcta tattttgcaa
    15121 aaagccaaac acctaaactg aggaatacta aggttgaacc accaccaaca aagacatcta
    15181 gcagtccgaa gctaaagaaa ttagcaatca tacaaccgat ggtaactccg atgatgtact
    15241 taggattgta aaaagccata aagttcatca tttctgaaat acgaaactga taagcaccat
    15301 agctgatggc atttaggggc ggtgtgacag tcaaaacgac gtagatagca gcgacgattg
    15361 caatatctgc aatatcacga atagttaatt ttttcatctt ttctcctttg gcggttgccc
    15421 gcgtgtaata tgcttggtga aagaagctaa gcaccaaggg ttgctttatt caaccctact
    15481 agtatagcac aatagcctgc tttatgctat acttaacaca tgaaaattcc tattcaaaaa
    15541 ttttttaaca atgaaatctt agcctatctc ttttttggcc ttgcgacaac tctagtttcg
    15601 attctctcac gactagtcat ttatcaacta agtcatcagg aactcctggc tactgcccta
    15661 gcaaatatta tcggtatcct atttgccttt atcacaaatg atacgattgt atttaagcaa
    15721 aaaaagaaaa atagacttat ccgtttagtg aaattttctc ttgctcgcct ttctactttt
    15781 ctgttagact tgtttttaac ttttctcttt gtaacccaat ttcctcacat tatagggcaa
    15841 ttcgtaactg aaaacattga taagataaat gctattgaaa caataattgc acaaatattc
    15901 attattatca tcaattatat attgagcaag gcctatattt ttagaaaata atgctccatt
    15961 tatgagcctt ttaattgcct tctataatga tttaggatac aatgaaacac gaaacattga
    16021 aaggaaaaca gtatgttaaa aaatctaaaa tcgttcttgc ttcgaggaaa tgttattgac
    16081 cttgctgtcg gtgttgtaat tgcctctgct tttggtgcta tcgttacttc acttgtaaac
    16141 gacattatca ctcctcttat tttaaatccc gctttgaaag ctgctaaagt tgaacgtatc
    16201 gctcaacttt cttggcatgg agtcggctat ggtaacttct taagtgctat tatcaatttt
    16261 atctttgtgg gtaccgccct cttctttatt atcaagggca ttgaaaaagc acagaaactg
    16321 actggcataa agaaagaaaa aactgccgaa aaaaaaccaa ccgaattgga agtccttcaa
    16381 gaaataaaag ctctccttga gaaaaaataa tcgattaaaa ggatctagcg caaagctaaa
    16441 tccttttttc tttactcttt gggtaacttg cctgcttttt caagcatttt tttgatcaga
    16501 taaggcatct taacattctc ccgccctttt ttctcaatca gttttttcac aaattctggc
    16561 atttgtaaat cttcagattc agctaaaatg tttttcgtct cgtctgaagg aactaactca
    16621 tcaaaggtct cttcttctgg gagaaattcc ttaaactctt gattttcatt gactctaact
    16681 gtattgacca aagcatcaat caaacgagaa tctgtatttg gcattggtgg acgatggtag
    16741 tttaccccaa attcctggca taagtcataa cattctacat cattgtcaaa caagacctca
    16801 atatgctcac tgatgaaact aataggtaca aaaatataat ggtctggatg ttctgtctgt
    16861 tctctgagat actccaaaac atctggttta atccatggaa taccaatatc actctcactc
    16921 tgccaagtgt tggtatattg ttctgaactc aagcccagtt tttcagcgac tagcttgcta
    16981 ttttcgaaaa tttggtctat ataaggatca ccaaagtcca aggcaaaaat gggcacacta
    17041 tgggctgaaa agatgacttt aaagctgtct tgctttactt cttcttttaa aatcttagca
    17101 atttcatctg cccaatagtt taagagcact tcttcctgat accagtcctt aatgaccaaa
    17161 aattgaattt gtttgctttc taaaaatttt tcatagccca tgacagagta gaaagaataa
    17221 tggggctcca aaatcaagca gatacattct tcaacaccat cagtttccat ctgcttgatc
    17281 acgtctggaa taaagggacg agaaaatttg ttggcaaagt agataccgta ctcctcccct
    17341 aaacgttctt tgactaaatt gacctcctca cgtgtaatcc gctgcaaagg cgtcccaccg
    17401 atacgaatat agttgtcata gagagtctgg atttcgtggt cttggggtct aactccacga
    17461 cgaatgtttg taaagaattc tgctactcct tcaaaactaa tctcctctgg agatccaaag
    17521 gtcatcatta atatggcttt tttcatgtta tcatccttat gatattattt tctctatcca
    17581 ttctatcatg aaagaaaaaa atagtaaacg tttcctacgt ctactatttc ttttgctctt
    17641 ttgttttttt atctcgtctt ataattagga tgataaggac aagaagtaag aggacgataa
    17701 taaggagtag aaaggctaga atgtaaatcc aatgactggg aattccgtga tcgaaatacc
    17761 gtgttcctac tgcttcccca ctttcatcct gtgctacttt caccggtgca tccgttattt
    17821 tagctaaaag gatatgtcgt ccattagtta tgttaaggaa acatctcgct tgtacttggc
    17881 tttacttagc agatattgat agtaagcctg gtgttcctca tccttggaac tcaaggttcg
    17941 ataaatctcc gtgtcatagg catccacttc taaaatccca aaaatttcaa gaccacgttc
    18001 tcgaccattg tagtagatag aaccgtagcg atgctggtcg aagaattcct tatcagcaaa
    18061 tttcttaatc tcaccgaaca tagcccctga agccatagag tgtccgtaga tgatcgtatt
    18121 aaaatcagta aagttgggat taaagcgata gtcgagaaaa attgatccca ttatcgtata
    18181 atcgccaaag gcattgcggt caaggtattt gacattatcc tgactttgta gaagcggata
    18241 gtctaccttg gtcccatcaa ggctaatcca accgcaaaca tctttgttca agtgctgtag
    18301 ggtttcaaaa cctgtgctcg aagcatcctt ttcaatagga ttatagtgat catagtcact
    18361 tgcttctact actctctgcc aactaaaaag agctacaaaa acacagaaga ataccagaaa
    18421 tacgttttta aatcgcgctc ttttttgaac cgtcatcagc actcctttct atgcgagatt
    18481 tcttaaaagg aaaggaggcg ggacaaaacc gcctcttttc tattcaccct tagtcagtaa
    18541 aagggtctgc ttcttgcatt ttttttactc tctcttctac gacgtaggat caagtagagg
    18601 acaaggagta agaaaatcaa gagcaagaac aatacaaaca agataaggta ccaaatccat
    18661 agtgggattg atgagaggaa acgtccaaga gacgagtcat caaagacact gtatggaaat
    18721 ggttttgatt tttttgtatg gaaagtattt ttagggacgg tgtctgtaat ctttgcgact
    18781 acgatatgac gaccattggt cacatcgaga aaacaggtac tgagtaggat gatacgatca
    18841 ctcggtgaga gtgagatatc ccgcttgtgc atggcgactg agagcaggtg gtctagatag
    18901 gcctgctggc ggtcctcacc ctgtattcct ggatcataga tgttaaagtc ataggcatca
    18961 acctccaaca tctcaaagat ctcgagccct ttttccacac cattgtagta tatactaccg
    19021 taacgatgct ggtcaaaaaa ttcctgatca gcaaacttag ccacatcacc aaacatgacc
    19081 ccattttcta cgtggtgccc gtagataatg gtattaaagt cttcgaactt aggattattt
    19141 cgtgcatcga gaaagatagc gcctgtcgct gcaaactcac ctttggagtc cttgttgaga
    19201 tacttttcat tgtccttggc ttggactaag ggatagtcga tattggtgcc atagacattg
    19261 atccaaccga ggacttcggg attgagttct tggagtttgc tgaagcctga aaaactggcc
    19321 agctcatcct gttgcgtcgt gacaggtcga taagcttcgt actcacttga cgaagctaag
    19381 gagtagactt ggttggaatc ccaaagggcg tagaaaccaa agagaaagat caaagctgca
    19441 aaaatgagca gcaagtgatt gatcacccca tccacagcaa gaacgagacg cctagacaga
    19501 ctcttttttg gtttctcacg cttggttttc atcatctgcc cccagccgtt taggcttctc
    19561 tacgtttttt agacaagaca acaaatccag ccaaaccaag accaatcatc aaaatgaatg
    19621 gaaggttatc aatcaagaga ccagtaggag taacgtcgtc gattttgtta gtaactttgt
    19681 tgtcgttttt cttttcacct atgaggatat ctgttaatgt aaaatctaca tttttttgtc
    19741 cttgagatgt ttgttctgca ccatttgtag tataaattga tgaatctgta taacctgcta
    19801 ctccagtttc agttactgta tatttagaac ctgctggtag ttgttcaact gtaaatgttt
    19861 caccatcttt aagagtaaaa ttgtactcaa ctgtatctgc tggggtcact actgccaaca
    19921 cctctgactt agatccatgt tttactataa tattcgtaac aggggtttct gctgttttat
    19981 ttgtacttgg aagttgaatc ttgatatgga attggaaatc tcttgtttta tcccctgaag
    20041 ctccatcaac ttttttagta attgttaatg ctttcgcatt ggggtctact tgactcggat
    20101 catcaggatt aggctcctta cttgcgtctt tggtatatgt gttatcaaat aaattataaa
    20161 cgccttgttc acttggttct actttagggg cgttagattt attattttcc ttaaagtata
    20221 ctgatgaaat atagacacca ccatttactt tattcttaac aataacgtgc atttcatatg
    20281 tacgtttgtc gtatctcatg gcatcaattg ttttaccatt tttggtaatc gcctgccatc
    20341 cagtttttgc ttccgctaca tcataaacgt attcaccagc atggccataa gcaactcccg
    20401 aaaaaatgtc ttctgttgct ttttgaatct tatcagcagt tgggtgatca tttttattgt
    20461 attctacatt tctatctgga attgcaactc cggtttgata tggcgcaccg ttagaagatt
    20521 gtcctgtttt ttctgtaaac ttaaatgtaa atgtcgctgt tggtgttgtt attccctctg
    20581 caatattcaa cgttttatta atcttcacaa cagcattatt ttcaccattt gctttggtta
    20641 ttgctgaatt atcatccgca aaagtcggga cacctctgaa tacggacaaa gcggttatag
    20701 ctgcaatgct tgcagtgaat agctttttaa agaatgtttt tttcatgatt atttcttttg
    20761 tttttttatt ttcttttttc attatgatca tcaaaagcct cgtcttctaa tgactgttac
    20821 aactttgcct tgagtatcct gtatagggat ggttccaaaa gcacgactgt ctacagccgt
    20881 tgttcgattg tccccgagga caaaaagttg tcctgctggg actttcatcg ggaaggttgc
    20941 cccttcctta tagagcagcg tttctttgta gatatcttgc tcttgttgag gagaaccgtt
    21001 gatgatgaga ccattttcgt ttatatcgat tgtacttcct tcggttgcta tgacacgcgc
    21061 cactctttcc ttacctttat aactatagac tagcaaatca ccaatcgaat agcgtttatc
    21121 caacctataa tagacgacca agtcgccatc ttttaaggcg ggcttcatgc catcgtcatt
    21181 gtatcttaat aatccaaaga cgaaaaagta aaggatggct agtacaaatc caaccatcag
    21241 aagtttactg actaagtaaa ggatatctcc ccagacactc ggcggagact tatcccgatt
    21301 gacttgtgtt actggtttct tatgtttctt tttaagcagc atcacttcct cctattcacc
    21361 acaaggtgac caagtaagat gataaagaag atccctccta gaggaagaac gactccaagc
    21421 acgagagtgc tactcaagaa gtcaattccc gtttcaggta cgctgttttt attattcgtg
    21481 actgtcgcag acttatttgc atcaagagtt ccttcttttt tatcatagga cacctgatac
    21541 ccacgagacg aacttgcttc ttcttcgata ctataacgag catttagagg aaggtcgaga
    21601 atcttgatgg ttttatcttt ctttagatca actgtcgcct taccattggt gaattgtagc
    21661 gttgtttttt gattatttac tattgcacta tacgatccat tcaatggttt attttgcgca
    21721 tcctttacgt taatggtaat cttaaaggat tgcagaagat tagcaaaagc acctgtaact
    21781 ttttttgtga ctgaaagtgt cggaagtctg tggttggtga cgattgtttg aacatcctct
    21841 gacaaagtcc cctcttgttt ttcataggtg acatgatatc ccgttctaga gttctctact
    21901 tcttccacct tatagtgggt atcacgaggc agataactga gggcagtttc ctgactattt
    21961 ttgagagaaa tctgtgcttt cccgttagtc actgttgtat tcccaaactt gccgttaatc
    22021 ggcttgccat ccttatctga aaaggtaaca ttaattgtga aagatttatt aggtgattgt
    22081 tcaccttcta ggattttctt tacggtgaga cttggaagct gtcggtaaat aactttgaag
    22141 gatggtttct ctttcgtacc tgtcttttga acttcgactg tgtatcccgg agcgtctggt
    22201 gctttcagtt cgtagtcata tcccttagct acctttgcaa aactagtatt ttcagtaaaa
    22261 ctattcgttt gaactggact agtgaccttt cgataggaat cctttatact tccatctttc
    22321 ttttggttta agtttaattc gacactaggt ggacgatttg ctgatggatt tgattttcca
    22381 tccacacctt tccactcaat ctctacagga accgtcaatg gatctgacgg cttgaaagtg
    22441 ggcttctcag aatagtcttt tattttggta cgtgaggtcc caataccata ggaataggca
    22501 acttttgccc ccttgttgga gtagagttta gtagcatcgg cttcttcggc atcatcacta
    22561 gtaagtgtct tatcacccgc aatcgcatca aatgcctctt ggctagaggt gacagtaaac
    22621 ttgagaacgt acttggcgtc atcctccaaa gtgtaactcg gattaaactt ggctgttact
    22681 tccactaatc cctgttcgtt cttcttagtt tcaatcgtaa cttgattgtc attcagttct
    22741 tgctcgttac catccttgat tttgacgaca cgaaggtgtg aagcatcacc aggtaacagc
    22801 tgcacgtact tggacaagac atctgatata gttacatggt gtacgcctag aggtagaatt
    22861 ttatctgtga tttctttgaa tttttgttgc aagtcatttt cattattggc agaaaatatt
    22921 tcgttgggga ttccagaatt ataaccactg atatatcctt taagagatgt gatactatcg
    22981 tttgaaccta tataatatct gaatttgatt gaataaaagc catctatttt tggagctatt
    23041 aaactagata tattcagcat tcctctttct aaaccagagg taaaccaata tggagtatca
    23101 atcatacgtc ccacatttgg attagaataa tagttatata ttgttctccc actattagac
    23161 tcgtaataca tattagcttc gccatctgac agcagaataa ctactttttt tgccccagcc
    23221 cttgcacttt gcaacaatct ttgagcttca gttaacccag catttatatt tgtcccagtg
    23281 ccaatgcccg cgtccatacc ataagaacga ctagagtcag caattgttaa cgaactaaca
    23341 atattttttg agctattaga atccgtgctc caagatacta aagttttagc atcatcatat
    23401 gaagagatat ttgaatacca accagctcaa tttggccaag ttccatattg ataactcaga
    23461 taatattcat tataataacg gtcattatac tgattatcaa tctttccggc aaaaccaact
    23521 atagacagac ggttttggga attagaaaga attgtatcaa tgagcccctg acgaccattc
    23581 acacctttca gtgtatgttt tagtgcttca atcctactaa tgtatcgtct actagagtaa
    23641 gaccacacat ctcgctcttc catactccct gaaagatcgg caaccaagac tacatccaag
    23701 ggttctgact gggtctccgt tcccagttta gatgtgatat ccagtgacaa ctcatactgg
    23761 tctttttgcc ctgatatcgg agtaatcgtt ttgtgcagag ttgtctgagg ttcagttatt
    23821 gaatctgcac gtaaagaatt aattggtgca cacaaaaaaa gaatagcgag aaagataact
    23881 aaactactgg taaaatattg aatcttctta aaatcatatt gaacgttcat gctatctccc
    23941 ttgctagaat atgttttaat ctgatactat ataaatctga gacttccata agataattgt
    24001 ataataatat taacttcgtg tcaattaatg tgtttgaaaa catgttatta taaattacaa
    24061 aaaagtatga ttcttctttg tttttcaact ttttctgtgg taaaatggaa aaaatagtaa
    24121 aaaatttcat aaaataaaga tgcatatatt tattctaatc gctcgaaaat ttcttttgaa
    24181 agtcttctga atttgaatta ttatattttt tcaaaattca taaaatttct caaatcatat
    24241 ttttccgaag ttttgtattc tattttatga taaaaaagga gtttttttat gacttatcca
    24301 aatctcttgg accgcttctt aacctacgtt aaggtcaaca cgcgctctga tgaacactct
    24361 actactactc caagtacaca gagtcaggtt gacttcgcaa caaatgtcct aattcctgaa
    24421 atgaaacgtg ttggactgca aaatgtttac tatctaccga atggttttgc tattggaacc
    24481 ttgccagcca acgatccgtc tttaacacgt aagattggtt ttatatcgca catggatact
    24541 gctgatttta atgctgaagg agtcaatcca caggtaattg aaaactacga tggtggtgtg
    24601 attgaactag ggaattctgg tttcaaactc gatccagccg acttcaagag tcttgaaaaa
    24661 tatccaagac aaacactcat cacaacagat ggaacaacct tgctaggtgc tgatgacaag
    24721 tcaggaattg ctgaaattat gacagccatt gaatatctaa ctgctcatcc tgaaattaag
    24781 cactgtgaga ttcgtgttgg ttttggtcca gatgaagaaa tcggtgttgg tgccaataaa
    24841 tttgatgcag aagattttga tgtggatttt gcctacactg ttgatggtgg tccactaggt
    24901 gaacttcagt acgagacttt ctcagccgct ggtgctgaat tgcatttcca aggccgtaat
    24961 gtccaccctg gtactgccaa agggcagatg gtcaatgccc ttcagctagc aattgatttt
    25021 cataatcaac ttccagaaaa tgaccgacct gagttaactg aaggttacca aggtttttac
    25081 catctaatgg atgtgacagg tagtgttgag gaggtgcgtg caagctacat cattcgtgat
    25141 tttgaaaaag atgcctttga agcgcgtaaa gcatccatgc aatctatcgc tgataagatg
    25201 aatgaagaac ttgggagcga ccgtgtcact ctcaacttga cagaccagta ctacaatatg
    25261 aaagaagtca ttgaaaaaga tatgactcca attaccattg ctaaagccgt tatggaagat
    25321 ctaggtatca cgcctattat cgaaccaatc cggggtggaa cagacggctc taagatttcc
    25381 tttatgggaa tcccaactcc gaatatcttt gcaggtggcg aaaatatgca cggacgtttt
    25441 gaatacgtta gccttcagac tatggaacgt gcagttgata ccatcattgg catcgtagct
    25501 tataaaggct aaaaagacga ggtggctcag ctacttcgcc tttcttttta ttctactggt
    25561 ttttcttgat ttccagtagt tgtagaagat tctgttgttt cattttctga agttgattca
    25621 gcaggtttag aatctcttgt attgcttggt ttgttttcgt cgctagcagt ttcaatgtta
    25681 gattctgcag ttgcgtttgg ttggttctca gcactggtgt tatcactatt tgcttcagca
    25741 tttcttgctg gacttgtttc ttcacttgcg ctaacttttg actggatttg atgattcaaa
    25801 actagaatag cttttgtcga ttcaagtaaa gctgttttgt ctttactatt agcagaaagt
    25861 tgatctaata atgcatccac cttatcaaag tccgcatcag atccattatt actttctaaa
    25921 taagagtgaa gcgacatgag aatatcgtag agtttttgat agagtacaag tgtctgagaa
    25981 tcttgctcag cattttcctt ttcttgttga agggcgctag cgatacgagt caaaacatct
    26041 tttacctgac tgtttacttc atccaagtct gcatcagcct tgtttgtggc agcttttaga
    26101 ttttctactt cttctgccaa ggattgtctg attccttctt catggatttg ttccaagagt
    26161 tgatttgcct tgttcaaaag actttctact tcttccttgc tatctgtcgc agattattgg
    26221 ttgctatcta ccatgtactc ctaaaacagg agagttataa tccaagatta caaggcctta
    26281 cagaaataag aaatccagat aagacaatgt tcgtccaaga cgctattcgc ttcgcacagc
    26341 agcacggatt caatatgctt taattttaaa gtttaggtgt caagacctct ttttagtgtg
    26401 cccaaaattt agagaagtaa tcaatcaact aacttttatt tttttcaaac tttcagtaaa
    26461 ctgacctaaa gctaactcaa tctgtctttg tagatgcttc tgctatcagc tagaagttga
    26521 tctacttttg ccaagactgc cttctcatca aaagttccag gttgatagtt ggattgcagg
    26581 gatggaatct tgtttttcaa agccgcttca tatcccttag tttgaacctt gatgtagtga
    26641 ttgtggtcgc catgaggaat cacaaaacct tctgaatctt cacttataat tcgattggca
    26701 tcaaaaacca taaccatctt cttcctcatg gtggacatgt agtgacggat tacttaatac
    26761 agaactagaa gaacttccta cctcttccgt gttagagtgt gatgggggat tgttaagaga
    26821 tgacttagga atatagtgat agtgttcccc atgtcttact atataagcat cacctatatc
    26881 tctgacaata tcattagggt taaagacata tgtggctgct aattcacctg ccgacaagtc
    26941 actctcagga atgaaatgat agtgaccacc atgtggtact attagtagat tgaaatagaa
    27001 tatgagcaaa ttgataaggg gattttaaag taatttctaa caatgattta gaaactatga
    27061 tgtgctattc taaattcaac tcactatata taaccatcat cggtagtata acgtccctgt
    27121 aattttgcta cagatacttc tgcactagct cctttatcgt ctttaccatg ttcttgtttt
    27181 tggcgattga tttcatcttt tgttcgtaca ttttctgcat gagcttgatc tttaaggtaa
    27241 acataatact ttccatctac cttaataata tatcctccct taacctaact gacaatatct
    27301 tgatctttcg gctgatagtt gggggctttc attaatggct cttcactaaa gagcgcatca
    27361 aaaggaactt taccattata gtagtgataa tgatcgccat gagaagttac ataaccttga
    27421 tctgtaatct taataacaat ttgttttgct tgaattcctt ctttttgact aacctagtct
    27481 ggagtcaaat tttcagtctt cttagtgtct ttattactgt ttacatatga aacacgattt
    27541 ttatctgtat tggcctgtta gctatgttgg ttcagagcat aaacacacag acttaaggaa
    27601 aggataacaa cagatccagc tgctatatat ttctttttaa atttcataat tacctcattt
    27661 ctataattat ttatatgatg tcttcattat taaatgatta tagaaattaa ttaaccaatt
    27721 aattaactag taaatattcc acctcttttt aagttgtatg tcaagaaatt ttatatatta
    27781 ataataaaat gaaattctcc caaagtcaga gttttatttc taacttttga gagaacttca
    27841 tttttgattc agactttttc tactgctatt ccttacgcta tgagatcaga taaattcttt
    27901 tttatcactt ctccacttgg caatcttaat tcaatcgttc catccatatt gaatataaca
    27961 ctatctaagc ctaatccgta actagctgta aatttttcta atttttcttg tacaggatct
    28021 actgctggag cttcctctaa tgctggatct aacatagggt cactccccac attcccttct
    28081 ggattcaaca ttccattatc cgttgagttt tctggtttta caggtttttc gtttggtgcc
    28141 tctggtaaag aatctgctgg tttattttct gttggttggt tctcaactgt tccagtagat
    28201 acttttccat tttcagatgg tttattttca ccatttcctt gaggtgcttc tcctgtaaaa
    28261 tctgccatat tctttttaat gacttctccc gatggtaaat ataattcaat tgttccgtcc
    28321 atattaaaca agacattttc tagcttcatc ccataacttt cagcaaattt tgctactttt
    28381 tcttgtacag gatccactgt aggaacttct tctaacgttg aattactagt actattccca
    28441 gtttcagaaa gtttttcttt ttctaccttc tcactagtct ttggttcttc taccttttca
    28501 tcaagttttg agttttcttg tgctttattc cttttaaatt gtggtagaat acttggttta
    28561 tcagtttgat tttctttttc caagataggt acttccacaa tataagtcga ttgattgtcc
    28621 aaataagcat ttgccatgaa ggttacagga attttatttc cggccgttct ggttgttcct
    28681 tggtttaatt tcggaatcgg taatttgatt tcaccaactt tatagttatt ttctaaataa
    28741 gcatttccat gaaattcatc aaacactctg actaaagcat cagttccttt aggcactgca
    28801 aattgagggt tcactcttaa ataagtatcc cctgcatgga aaggatagaa aatcgtttga
    28861 ctggccattt tgtaagctaa agaggttgga actgtaaatg taccatcata acttacttct
    28921 ggataatctt ttgaagcgat agtatactta aatgtttgtc ctggtaaata aggttgatct
    28981 aattcaaagt ttgcaatatt ccctactcct tctccaaata ctttaccaga tactttctcc
    29041 aatacttttc catctggtgt tattaatttt actagcatat tgatacctaa ttttttctcc
    29101 aattcaggcg gaaaactaaa agaaacgcgt ttttgaccat tggctagagt aaagttttga
    29161 ttattaaacg tactattttt taacaaatta acaacattcg ttaattcttc tccagtataa
    29221 actttattcc cttctttttt agcaactcct tcttcgggtt taaacagttc atagttactg
    29281 tgagaatgac caattccaac cggtttatgt tcatcaatcg gatctgcatg atggtgatct
    29341 ccatgcggat aaataatcgc atttttttct ttattcacga caatactttc acgtttgaca
    29401 ccatattgtt tcataatgcc agcaattttt tcttcgattt ttttatctaa atctttcatt
    29461 tctttggcat tacttggata atcctgttca tgagatgaca aagaatctaa tccattatga
    29521 ctagttttaa cttcctctaa atgtttttgc gcagccttaa tttgctcttc tgtcaagtcc
    29581 ttcttgaaga aataatgatt gtggtctccg tgactcatga caaaacctga ttcatcttca
    29641 gcgataatac gattagcatc aaatccgtat ccatcttctt catgtttctc atgtgaagtt
    29701 cctggattga ttggaagaga tggagaaggt gttgctagac tattgtttgg aagagtcggt
    29761 tgcccaattt gatttgattt tggaatgtaa tggaaatgat caccatgtct tacaatataa
    29821 gctgtagccg tttcttcaac gatatctttt ggattaaaaa tataaccatc agatgctgaa
    29881 gagagctcct tacttgtcgt taaagaagaa ggattgcttg aaagactgcc tagactagac
    29941 actacttcat taggttttgc atttgtagaa actgtagaac cagttccact gataggcacc
    30001 attctggcaa tcttttcttc taaggcagaa agcttgctgt aaggaataaa gtggtaatgg
    30061 tcgccatgcg gaatcgcaac tccatttggt gtacgactga taatcttagc agggtcaaag
    30121 accaggccat ctgattcact gtaacgttgg gcgctaggtg aatcatagag ttccttcaaa
    30181 agactctgga gattttcaga tttatttgct ggcttgctag ttgatccttt tgctacagat
    30241 tgcgtgttat tgtcactagc tgttgaagaa tagcttaact gactcggttg catatttttt
    30301 ccagccagat gtgctttagc tgctgctaat tcactagcag ataaatcgct tttgggaatg
    30361 tagtgatagt gacctccatg aggaacgata taagcattac ccgtatcttc gataatatca
    30421 gctggattaa agacataacc atcatttgtc gtatatcgtc cctgagacct tgctacagca
    30481 acattagagt taaccttctc attatctttg acatgttctt gtttttgacg attgatttca
    30541 tctttagttc gaacattatc agcatgagct gcatctttca ggtagacata atattttcca
    30601 tcgaccttga tgatataacc acccttgact tcattgacaa tatcagcgtc tttaagttga
    30661 tagtttggat ccttcatcaa gagttcttca ctaaagaggg catcataagg aactttccca
    30721 ttatagtaat gatagtggtc accgtgtgac gttacatagc cctgatctgt aattttgatt
    30781 acaatttgct cggcctgaat tccttctttc tggctaatct ggtctggtgt caagttttca
    30841 cttttctgac ttgactggct gccatctaca taagagacac gattattgtc cttattttcc
    30901 tgcgaacgat gctggtttag tgcataggca catagactca aggatacgat aacagctgat
    30961 ccagctgcta tatatttttt actgaatttc ataaatccct ca
//
LOCUS       DAVBCB010000021        30864 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVBCB010000021 DAVBCB010000000
VERSION     DAVBCB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.021"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..83)
                     /locus_tag="VQU28_000929"
     CDS             complement(<1..83)
                     /locus_tag="VQU28_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HET2476301.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEE"
     gene            78..332
                     /locus_tag="VQU28_000930"
     CDS             78..332
                     /locus_tag="VQU28_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2476302.1"
                     /translation="MHRTTIPYFVSQEVYWKVRNIGAEAIRRNCERGAIFSGKIKHHE
                     DSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            676..2010
                     /gene="trmFO"
                     /locus_tag="VQU28_000931"
     CDS             676..2010
                     /gene="trmFO"
                     /locus_tag="VQU28_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HET2476303.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRIFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2094..2831
                     /locus_tag="VQU28_000932"
     CDS             2094..2831
                     /locus_tag="VQU28_000932"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HET2476304.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            2840..3397
                     /gene="frr"
                     /locus_tag="VQU28_000933"
     CDS             2840..3397
                     /gene="frr"
                     /locus_tag="VQU28_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HET2476305.1"
                     /translation="MANAIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRIHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVV
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3457..4311
                     /gene="cvfB"
                     /locus_tag="VQU28_000934"
     CDS             3457..4311
                     /gene="cvfB"
                     /locus_tag="VQU28_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HET2476306.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTSLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4320..4535
                     /locus_tag="VQU28_000935"
     CDS             4320..4535
                     /locus_tag="VQU28_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HET2476307.1"
                     /translation="MRKSFYTWLMTERNPKSNNPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4621..5589
                     /locus_tag="VQU28_000936"
     CDS             4621..5589
                     /locus_tag="VQU28_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HET2476308.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMALVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            5769..6269
                     /locus_tag="VQU28_000937"
     CDS             5769..6269
                     /locus_tag="VQU28_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476309.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6272..6532
                     /locus_tag="VQU28_000938"
                     /pseudo
     CDS             6272..6532
                     /locus_tag="VQU28_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069408.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(6899..8010)
                     /gene="ald"
                     /locus_tag="VQU28_000939"
                     /pseudo
     CDS             complement(6899..8010)
                     /gene="ald"
                     /locus_tag="VQU28_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253293.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8187..8756
                     /locus_tag="VQU28_000940"
     CDS             8187..8756
                     /locus_tag="VQU28_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476310.1"
                     /translation="MESIFVKFAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLANPLGRWGIELKDNGKFIGTIDLHKIDPVLKKAAIGYI
                     INKKYWNQGLTTEANRAVIELAFEKIGMNKLTALHDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            8824..9474
                     /locus_tag="VQU28_000941"
     CDS             8824..9474
                     /locus_tag="VQU28_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HET2476311.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTSVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9458..11698
                     /locus_tag="VQU28_000942"
     CDS             9458..11698
                     /locus_tag="VQU28_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HET2476312.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSVSYFALLGFVFLLVCL
                     FIQFPWKSAGKVLVICGVFGFWFLFQTWQQSQVSQNLVDSVERVRILPDTIKVNGDSL
                     SFRGKSDGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFDYQGY
                     LKTQGIYQTLNIKRIQSLQKVGSWDIGENLSSLRRKAVVWIKMHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMDGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVTSESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     VFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVASRPLVFGQPNEWLLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIKKWQEKMTTSNAQRSLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEMTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMIVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            11878..12066
                     /locus_tag="VQU28_000943"
     CDS             11878..12066
                     /locus_tag="VQU28_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476313.1"
                     /translation="MTVTKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            12098..12685
                     /locus_tag="VQU28_000944"
     CDS             12098..12685
                     /locus_tag="VQU28_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476314.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12689..13864
                     /locus_tag="VQU28_000945"
     CDS             12689..13864
                     /locus_tag="VQU28_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476315.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILALFVSVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWKDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLPKQIDNVDLNWSMGMVLLPCLIIFLLLGILFIERWGSSQKKRIF"
     misc_feature    14038..14169
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14185..14715
                     /gene="infC"
                     /locus_tag="VQU28_000946"
     CDS             14185..14715
                     /gene="infC"
                     /locus_tag="VQU28_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HET2476316.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14748..14948
                     /gene="rpmI"
                     /locus_tag="VQU28_000947"
     CDS             14748..14948
                     /gene="rpmI"
                     /locus_tag="VQU28_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HET2476317.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15000..15359
                     /gene="rplT"
                     /locus_tag="VQU28_000948"
     CDS             15000..15359
                     /gene="rplT"
                     /locus_tag="VQU28_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HET2476318.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15417..15797
                     /locus_tag="VQU28_000949"
     CDS             15417..15797
                     /locus_tag="VQU28_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HET2476319.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16047..16847
                     /locus_tag="VQU28_000950"
     CDS             16047..16847
                     /locus_tag="VQU28_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HET2476320.1"
                     /translation="MNPTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVNQRVCEDGPVFRTGTVVL"
     gene            16810..17796
                     /locus_tag="VQU28_000951"
     CDS             16810..17796
                     /locus_tag="VQU28_000951"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HET2476321.1"
                     /translation="MVLFSAQEQLYYKEKIMTTNRLQVSLPGLDLKNPIIPASGCFGF
                     GQEYAKYYDLNLLGSIMIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAE
                     KLPWLEREYPNLPIIANVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGL
                     LIGQDPDLAYDVVKAAVEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVG
                     MRFDLKTRKPILANGTGGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEM
                     YLAGASAIGVGTANFTNPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            17838..19739
                     /locus_tag="VQU28_000952"
     CDS             17838..19739
                     /locus_tag="VQU28_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HET2476322.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYM
                     AKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWL
                     DKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIP
                     VASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINN
                     SLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYL
                     SAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMK
                     INEKLGGKD"
     gene            complement(19800..21455)
                     /gene="pavA"
                     /locus_tag="VQU28_000953"
     CDS             complement(19800..21455)
                     /gene="pavA"
                     /locus_tag="VQU28_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HET2476323.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPLANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21560..22057
                     /gene="ybeY"
                     /locus_tag="VQU28_000954"
     CDS             21560..22057
                     /gene="ybeY"
                     /locus_tag="VQU28_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HET2476324.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLGYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22038..22433
                     /locus_tag="VQU28_000955"
     CDS             22038..22433
                     /locus_tag="VQU28_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HET2476325.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFGFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22450..23349
                     /gene="era"
                     /locus_tag="VQU28_000956"
     CDS             22450..23349
                     /gene="era"
                     /locus_tag="VQU28_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HET2476326.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPDRFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23398..24222
                     /gene="mutM"
                     /locus_tag="VQU28_000957"
     CDS             23398..24222
                     /gene="mutM"
                     /locus_tag="VQU28_000957"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HET2476327.1"
                     /translation="MPELPEVETVCRCLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQSALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARSSQTLTAEEATAIHDQTIAVLSQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQGCVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24222..24827
                     /locus_tag="VQU28_000958"
     CDS             24222..24827
                     /locus_tag="VQU28_000958"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HET2476328.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKPGG
                     RLFEALVQHFWQEIILENGELNRPLLASLIFSNPDEREWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            24814..26013
                     /locus_tag="VQU28_000959"
     CDS             24814..26013
                     /locus_tag="VQU28_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HET2476329.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSEQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26013..26162
                     /gene="rpmG"
                     /locus_tag="VQU28_000960"
     CDS             26013..26162
                     /gene="rpmG"
                     /locus_tag="VQU28_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2476330.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26202..26435
                     /gene="secG"
                     /locus_tag="VQU28_000961"
     CDS             26202..26435
                     /gene="secG"
                     /locus_tag="VQU28_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HET2476331.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26537..28891
                     /gene="rnr"
                     /locus_tag="VQU28_000962"
     CDS             26537..28891
                     /gene="rnr"
                     /locus_tag="VQU28_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HET2476332.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            28854..29321
                     /gene="smpB"
                     /locus_tag="VQU28_000963"
     CDS             28854..29321
                     /gene="smpB"
                     /locus_tag="VQU28_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HET2476333.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29337..30197
                     /gene="tehB"
                     /locus_tag="VQU28_000964"
     CDS             29337..30197
                     /gene="tehB"
                     /locus_tag="VQU28_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HET2476334.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEDVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30270..>30864
                     /gene="coiA"
                     /locus_tag="VQU28_000965"
     CDS             30270..>30864
                     /gene="coiA"
                     /locus_tag="VQU28_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HET2476335.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQLHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLTRLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQG"
BASE COUNT         9168 a         5715 c         7071 g         8910 t
ORIGIN      
        1 atctcttcaa aagtcgtgcg ctgaacacca acaagacgct taaatcgtgc atcagttagt
       61 tgtttacttg cttcataatt catagaacta ctataccata ttttgtttcg caggaagtct
      121 attggaaagt aagaaatatt ggagctgagg cgattagaag aaattgtgag cgtggtgcta
      181 ttttttcagg taaaataaaa catcacgaag attctcagtt taaaggagac cactatgttg
      241 aatgttatgc tgttttagat aatacggtta tagcaagaga tagaataaca gtccctatcg
      301 atccgttatg tggaaaagat tttatagagt agcatataat tgattcttaa ctggaatact
      361 ctctatctct ttacatcaag aaaatgacta aacagggaag tttgccttct tccctttttt
      421 tgttatacta gtagaagaaa aaaatagaaa gatttgtggg agtgaaaaag tccgggggac
      481 tttttcagct tgagccaaga aattcgagag cgaggaaagt ccgggggact ttttcagcct
      541 gagccaagaa attcgagagc gaagtaagtc caatggactt tttcagcctg agccaagaaa
      601 ctcgaaagct cgtaagtctg attggctttt tcaatgtgaa tcttaacttc acactcccaa
      661 agaggtatta gtgtcgtgtc tcaatcttat atcaatgtta tcggtgctgg tttggcaggt
      721 tctgaagcag cttaccaaat cgcagagcgt ggtattccag ttaaactata tgaaatgcgt
      781 ggtgtcaagt cgactcccca gcataaaaca gacaattttg ctgagttggt ttgttccaat
      841 tctttgcgtg gggatgcttt gacaaatgca gttggtcttc tcaaggaaga aatgcgtcgc
      901 ttgggttctg ttatcttgga atctgctgag gctacacgtg ttcctgcagg tggtgccctt
      961 gcagtggacc gtgatggttt ctctcaaatg gtgaccgaaa aagttgccaa ccaccccttg
     1021 attgaagtgg ttcgtgatga aattacagaa ttgccgacag atgttattac ggttatcgct
     1081 actggtcctt tgacaagtga tgccttggct gaaaagattc atgcccttaa tgacggtgat
     1141 ggtttttatt tctacgatgc ggcagcgcct attatcgatg tcaacactat cgatatgagc
     1201 aaggtctacc tcaaatcacg ttatgataag ggagaagcgg cctacctcaa tgcccctatg
     1261 accaagcaag aatttatgga tttccatgaa gctttggtca atgcagaaga agcaccgctt
     1321 aattcttttg aaaaagaaaa gtactttgaa ggctgtatgc ctatcgaagt catggccaaa
     1381 cgtggcatta aaactatgct ttatggccct atgaagccag tcggtcttga gtacccagac
     1441 gactatacag gacctcgtga tggagaattt aaaacacctt atgcggttgt gcaacttcgt
     1501 caggacaatg cagctggtag cctctacaat attgttggtt tccagaccca cctcaaatgg
     1561 ggagaacaaa agcgtatctt ccaaatgatt ccgggtcttg aaaatgcaga gtttgtccgt
     1621 tatggtgtca tgcatcgcaa ttcttacatg gattcaccaa atcttcttga gcagacttac
     1681 cgttctaaga aacaaccaaa tctcttcttt gctggtcaga tgacgggggt ggaaggctat
     1741 gttgagtcgg cggcttcagg cttagttgcg ggaattaacg cagctcgtct cttcaaggaa
     1801 gaaagcgagg ctattttccc cgagacgaca gcgattggaa gcttagctca ttacattacc
     1861 catgccgaca gcaaacattt ccaaccaatg aatgtcaatt ttgggatcat caaggagttg
     1921 gaaggcgagc gtatccgtga taagaaggct cgttatgaaa aaattgcaga gcgtgccctt
     1981 gccgacttag aggaattttt gactgtctaa tttttttgaa agaattgctc atgatactat
     2041 aaaaatctta gaaattgtga taaaataggt aggatgaaag aaggagagtg aaaatggcga
     2101 atcccaagta taaacgtatt ttaatcaagt tatcaggtga agcccttgcc ggtgaacgtg
     2161 gcgtagggat tgatatccaa acagttcaaa caatcgcaaa agagattcaa gaagttcata
     2221 gcttaggtat cgaaattgcc cttgttattg gtggaggaaa tctctggcgt ggagaacctg
     2281 cagcagaagc aggtatggac cgtgttcagg cagattacac tggaatgctt gggactgtta
     2341 tgaatgctct tgtgatggca gattcattgc aacaagttgg ggttgatacg cgtgtacaaa
     2401 cagctattgc tatgcaacaa gtggcagagc cttatgtccg tggacgtgcc cttcgtcacc
     2461 ttgaaaaagg ccgtatcgtt atctttggtg ctggaattgg ttcaccatac ttctcgacag
     2521 atacaacagc ggcccttcgt gcagctgaaa tcgaagcgga tgccatcctc atggctaaaa
     2581 atggtgtcga tggtgtttac aatgccgatc ctaagaaaga taagacagct gttaagtttg
     2641 aagaattgac ccaccgtgac gttatcaata aaggtcttcg tatcatggac tcaacagctt
     2701 caaccctctc aatggacaac gacattgact tggttgtctt caacatgaac caatcaggca
     2761 acatcaaacg tgtcgtattt ggtgaaaata tcggaacaac agtttcaaat aatatcgaag
     2821 aaaaggaata agaaagaata tggctaacgc aattattgaa aaagctaaag agagaatgac
     2881 ccagtctcac caatcacttg ctcgtgaatt tggtggtatc cgtgctggtc gtgccaatgc
     2941 aagcttgctt gaccgtatac atgtagaata ctatggagtt gaaactcctc ttaaccaaat
     3001 cgcttcaatt acgattccag aagcgcgtgt tttgttggta acaccatttg acaagtcttc
     3061 attgaaagac atcgaacgtg ccttgaacgc ttctgatctt ggtatcacac cagctaatga
     3121 cggttctgtg attcgcttgg ttgtcccagc tcttacagaa gagactcgtc gtgaccttgc
     3181 taaagaagtg aagaaggtcg gcgaaaatgc taaagtggct gtccgcaata tccgtcgcga
     3241 tgctatggac gaagctaaga aacaagaaaa agcaaaagaa atcactgaag acgaattgaa
     3301 gactcttgaa aaagacattc aaaaagtaac agacgatgct gttaaacaca tcgacgatat
     3361 gactgccaac aaagagaaag aacttttgga agtctaaaaa taaacagaaa aactcagttg
     3421 gcattgctgg ctgagtttta ttcgaaagaa ggaaatatga atacaaatct tgcaagtttt
     3481 atcgttggac tgatcatcga tgaaaacgac cgtttttact ttgtgcaaaa ggatggtcaa
     3541 acctatgctc ttgctaagga agaaggccaa catacagtag gggatacggt caaaggtttt
     3601 gcatacacgg atatgaagca aaaactccgc ctgacaagct tagaagtgac tgccactcag
     3661 gaccaatttg gttggggacg tgtcacagag gttcgtaagg acttgggtgt ctttgtggat
     3721 acaggccttc ctgacaagga aatcgttgtg tcactcgata ttctccctga gctcaaggaa
     3781 ctctggccta agaagggcga ccaactctac atccgtcttg aagtggataa gaaagaccgt
     3841 atctggggtc tcttggctta tcaagaagac ttccaacgtc ttgctcgtcc tgcctacaac
     3901 aacatgcaga accaaaactg gccagccatt gtttaccgtc tcaagctatc aggaactttt
     3961 gtttacctac cagaaaataa tatgcttggt tttattcatc ctagcgagcg ttacgcagag
     4021 ccacgtttgg ggcaagtatt agatgcgcgc gttattggtt tccgtgaagt ggaccgcact
     4081 ttgaacctct ccctcaaacc acgctccttt gaaatgttgg aaaacgatgc tcagatgatt
     4141 ttgacttatt tggaaagcaa tggcggtttc atgaccttaa atgacaagtc atctccagac
     4201 gacatcaagg caacctttgg catttctaaa ggtcagttca agaaagcttt aggtggtctt
     4261 atgaaggctg gtaaaatcaa gcaggaccag tttggtacag agttgattta gggaggctta
     4321 tgagaaaatc attttacact tggctcatga ccgagcgcaa tcctaaaagt aacaatccca
     4381 aagcaatttt ggcagacctc gcttttgaag aggcagcttt tccaaaacat acagatgatt
     4441 ttgatgaggt cagtcgcttt ttggaggagc atgccagttt ctcttttaac ctaggagatt
     4501 ttgacagcat ttggcaggaa tatctagaac actagcattt attcattggg tttgggctag
     4561 taatttctcc atccctctgc tataataaaa agaaataaaa ggattagaga ggttctttat
     4621 ttgaaggaac attcaataga cattcaactg agtcatccag atgacctgtt tcatcttttt
     4681 ggttccaatg aacgccatct tcgtttgatg gaagaagagc ttgatgttgt gattcatgct
     4741 cgtacggaga ttgtccaggt tttgggagaa gagtctgctt gcgaggaagc ccgtcaggtt
     4801 attcaagctc tcatggcctt ggtaaatcgt gggatgaccg ttggtacgcc agatgtagtg
     4861 acggctatta gtatggtcaa aaatgatgaa attgacaagt ttgtcgccct ttacgaagaa
     4921 gaaatcatca aggataatac tgggaagcct atccgcgtca aaaccttagg tcaaaaactt
     4981 tatgtggaca gtgtcaaaca gcatgatgtg acctttggaa ttgggccagc aggtacaggg
     5041 aagaccttcc ttgcagtgac cttggcagtg actgccctta aacgtgggca agtcaagcga
     5101 attatcctaa ctcgtccagc ggtggaagcg ggagagagtc ttggatttct tccgggtgat
     5161 cttaaggaga aggtggatcc ttaccttcgt cctgtttacg atgccttgta tcaaattctt
     5221 gggaaagacc aaacgactcg tctcatggag cgtgaaatta tcgaaattgc gccccttgcc
     5281 tatatgcgtg gccggacctt ggatgatgcc tttgtcattc tcgatgaggc gcaaaacacg
     5341 accatcatgc agatgaagat gttcttgacg cgtttaggct ttcattctaa gatgattgtc
     5401 aatggagata tcagtcagat tgacctgcca cgtaatgtca agtccggttt gattgatgct
     5461 caagagaaac tcaagaacat ccatcagatt gactttgttc atttttcagc caaggatgtg
     5521 gttcgccatc cagttgtcgc tcagattatc cgagcctatg aatattctac tgaagttgca
     5581 cacgactgat tttgaggaag ttcgcctgca aaagaatata cttttgagag aggaaaatcc
     5641 agttgtatag gctaaaggtt tgagacaacg attaggcacg atggaaagaa cttttatgtg
     5701 gctgatgacg atcagtgcat cttcctgtgt cataatcaca gggcacaaga aagtaggaat
     5761 ttgaaaagat gattgaccaa ctatctaagt attacagttg taggatacta actgaaaagg
     5821 atattccaag tattttatct ttatatgaaa gtaatcctct gtattttcag cattgtccac
     5881 cagagccaaa ttttgcaact gtaaaagagg acatgctttg tctacctgaa ggtaaagcta
     5941 aggctgataa gttttttgtt ggattttgga atggctctga ccttgtggct gttatggatt
     6001 ttgtctatgc atatcctgat gaggagactg tttttattgg tttgtttatg gttgatcaag
     6061 cctatcagag gaaagggatt ggtagtcata ttgtgacaga agcactagct tattttgcta
     6121 agaactttcg aaaggcacgt ttggcttatg ttaagggaaa tccgcaatct cagcattttt
     6181 gggaaaagca gggctttaaa tcaattggat gcgaggttaa gcaagaactc tatacggttg
     6241 ttatcgctga acagagccta gaagattaga aatggcatca agtaagaact atttggaatt
     6301 tgttttggaa caattatcag gattagatga tgtgacttac cgttccatga ttggggagta
     6361 tattctttac ttccgcggca agattattgg cggcatttat gacgatcgct ttttagttaa
     6421 acccgtgcaa gcagtcttag ataagattga ccaatcttct tttgagtttc catacaaagg
     6481 tgccaaagaa atgatttgag tggaagaact tgataataag atgtttctat aagacctgat
     6541 tttagctatg tataaccaac tgccaacgcc caaacctaaa aagaaaaagc aagggtgaac
     6601 gaagtaaaaa agaagtctgc taaggccctg tctttgcacg ggtaaaattt tatatataaa
     6661 aagaagctgg gactaaagag ctcagcttcc tttggtttat ataattgtca ttacaagacg
     6721 aagtggttgg gcgaaactct gttgacttta ttcaatttag agtttcttat gcacaattga
     6781 gtctggaacg aaagtctcca gttgcaaagt atacagtaca ataaaccaac gatgtaatag
     6841 ctgatgacac aaagcacagt gggtaggact tgcgaagtca cccttttctt ttcaaaattt
     6901 atactaaatc attgatatca gtgtagtcac gattaagtcc ttgagcaact ggtaggctag
     6961 tcaagtaacc ttgataagtg gtcacacctt gacgcaagcc ttcatcttca gagattgctt
     7021 gtgcgaatcc tttgccagcc aaagcttcga tataaggaag agtgacattg gttagggcga
     7081 tggttgaagt gcgggcaacc gcaccaggga tattggcaac ggcatagtgg agaacaccgt
     7141 gtttttcata gacgggttca tcgtgcgttg tcacacggtc agctgtttcg ataacgccac
     7201 cttggtcaac agcaacgtca acgatacaga gcctggacgc atttgtttga ccatctcatc
     7261 tgtcaccaat tccggtgctt ttgcaccagg gatgagaatg gctccaatca ccacatcagc
     7321 atctctcaca cttgcttcaa tgttgaatga attagatata atagtttgaa tttgacttcc
     7381 aaagacttct tctagaactg agagacgctt ggcactaata tctaaaatag tcacttgagc
     7441 accaagacca agggcgatgc gggcagcatg tgtaccgacg acaccatcac cgatgatagt
     7501 tacttttcct tttggaacac ctggtacacc accaagtaga acaccagagc caccagcttg
     7561 cttagtaagg aagtgagctc cgatttgaac agccatacga cctgcaacct cactcatagg
     7621 aacgaggagc ggtagttgtc cttgattgtc acgaacagtt tcataggcaa ttcctgttgt
     7681 ttttgctgct aacatagcat ctgctaattc tggagcagcg gccatgtgca agtaggtgaa
     7741 gagaagaaga tcgtcgcgca agtaaccgta ttcagaactt aaaggttctt ttactttcac
     7801 aaccaactct gctgcccaag cttcaccagc agtagcgaca atctcagctc cttgcttttg
     7861 atagtcagca tcagtaaagc cagaaccgag accagcattt gtttcgataa ggacacgatg
     7921 accacgacta actaagctat gaacacctgc aggtgtgagg gcgacacggt tttcgttatt
     7981 tttaatttct tttgggattc cgattaacat tgagataacc tacctttcaa ttgacggtct
     8041 tgttttggtt gtcacattcc agttcataaa tcaaaaatgt gacggtttca ttgtatatga
     8101 aaccgcttca aaaatcaaga aaaacttgtc atccaaattt ttttatgcta gactagtgaa
     8161 aatcaagctc taatggaggg aaaagtatgg aatcaatatt tgtgaaattt gcccagtatc
     8221 cgtctataga aacggagcgt ttattgctta gacctgtaac tttggatgat gcggaagcaa
     8281 tgtttgacta tgcctcggac aagggtaata cacgttacac ttttccaacc aatcaaagct
     8341 tggaagaaac caagaataac attgctcagt tctacttggc taatcccttg ggacgttggg
     8401 ggattgaatt aaaagataac ggcaagttta tcggaactat tgacttacac aagattgatc
     8461 ctgttcttaa gaaggcagct attggctaca ttatcaataa aaagtattgg aatcaaggat
     8521 taacgacaga agccaatcgt gctgtgattg agctagcttt tgagaagata gggatgaata
     8581 agttgactgc ccttcacgat aaggctaatc ccgcgtcagg aaaggtcatg gagaaatcag
     8641 gcatgcgttt ttcccatgca gaaccatatg cttgtatgga ccagcatgaa aaaggccgaa
     8701 tcgtgacaag agttcattat gtcttgacca aggaagacta ttttgcaaat aaataagcag
     8761 ttgaaaagaa atttttcgac tgttttttct tcctcttacg aataatctaa gagaggagaa
     8821 aatatggaag caattatcga gaaaatcaaa gagtataaaa tcatcgtcat ctgtactggt
     8881 ctgggcttgc ttgtaggagg atttttcctg ctaaagccag ctccacaaac atctgtcaaa
     8941 gagacgaatt tgcaggctga agttgcagct gtttccaagg actcatcgac cggaaaggaa
     9001 gtgaacaagg aagagaagga agaaccagtt gaacaagatc taatcacagt agatgtcaaa
     9061 ggtgctgtca aatcgccagg gatttatgac ttgcctgtag gtagtcgagt caatgatgct
     9121 gttcagaagg ctggtggctt gacagagcaa gcagacagca agtcgctcaa tctagctcag
     9181 aaagttagtg atgaggctct ggtttacgtt cctactaagg gagaagaagc agttagtcaa
     9241 cagactggtt cggggacagc ttcttcaaca agcaaggaaa agaaggtcaa tctcaacaag
     9301 gccagtctgg aagaactcaa gcaggtcaag ggactgggag gaaaacgagc tcaggacatt
     9361 attgaccatc gtgaggcaaa tggcaagttc aagtcagtag acgagctcaa gaaggtctct
     9421 ggcattggtg ccaagaccat agaaaaactt aaagactatg ttacagtgga ttaagaattt
     9481 ctctattccc ctaatttacc tgagttttct attactatgg ctttactacg ccattttctc
     9541 agtatcctat tttgctttgt tgggttttgt ttttctgcta gtctgcctct ttatccaatt
     9601 tccttggaaa tcagcaggta aagttctagt gatttgtgga gtctttggct tctggtttct
     9661 gtttcaaact tggcaacaga gtcaagtgag tcaaaatttg gtggattctg ttgaaagggt
     9721 acggatttta ccagacacta ttaaggttaa cggtgacagt ctgtcctttc gtggtaagtc
     9781 tgatggtcgt gctttccaag tctattataa actccagtcc gaggaggaga aagaagcctt
     9841 tcaagcttta actgaccttc atgagatagg actagaaggg aagctttcgg agccagaagg
     9901 gcagagaaat tttggtggct ttgactacca aggctatctg aagactcagg gaatttacca
     9961 gacactcaat attaaaagaa tccagtcact ccaaaaggtt ggcagttggg atataggtga
    10021 aaacctgtcc agtttacgtc gaaaggctgt ggtttggatt aagatgcact tcccagaccc
    10081 tatgcgcaat tacatgacag gactcttgct aggacatctg gacaccgatt ttgaggagat
    10141 gaatgagctt tattccagtt taggaattat tcaccttttt gccttgtcag gtatgcaggt
    10201 agggtttttc atggacggat ttaagaaact acttttacga ttgggcttga cacaagaaaa
    10261 gttgaagtgg ctgacttatc ccttttccct tatctatgcg ggactaactg gattttcagc
    10321 atcggttatt cgcagtctct tgcaaaagct actggctcaa catggggtta agggcttgga
    10381 taattttgcc ttgacggtgc ttgtcctctt tattgtcatg ccaaactttt tcttgacagc
    10441 aggaggagtc ttgtcctgtg cttatgcttt tatcctgacc atgaccagca aagaagggga
    10501 ggggctcaag gctgttacta gtgaaagtct agtcatctcc ttgggcatat tgcccattct
    10561 atccttctat tttgcggaat ttcaaccttg gtctatcctt ttgacctttg tcttttcctt
    10621 tctttttgac ttggtcttct taccgctctt gtctatctta tttgtccttt cctttctcta
    10681 tccagtcatt cagctgaact ttatctttga atggttagag ggcattattc gcttggtctc
    10741 gcaggtggca agtagaccgc ttgtctttgg acaacccaat gaatggcttt taatcctatt
    10801 gttaatttct ttggctttgg tctatgattt gaggaaaaac attaaaaagc taacggtatt
    10861 gtgcttattg attacagggc tctttctcct gaccaagcat ccactggaaa atgaaatcac
    10921 catgctggat gtggggcaag gagaaagtat tttcctacgg gatgtaactg ggaaaaccat
    10981 tctcatagat gtaggtggta aggcagaatc ttataagaaa atcaaaaaat ggcaagaaaa
    11041 gatgacgacc agcaatgccc agcgaagctt gataccctat ctcaaaagtc gaggagtagc
    11101 taagattgac cagctaattt tgactaacac ggacaaggag catgttggag atttgtcaga
    11161 gatgaccaag gctttccatg taggggagat tctagtatca aaagacagtc tgaaacagaa
    11221 ggaatttgtg gcagaactac aggcgactca aacaaaggtg cgtagtatga tagtagggga
    11281 gaacttaccc atttttggaa gtcagttaga agttctatct ccaaggaaaa tgggagatgg
    11341 aggacacgat gataccctag ttctgtatgg gaaattcttg gataagcaat ttctcttcac
    11401 gggaaatttg gaggagaaag gagagaagga cttgctgaag cactatccag acttgaaagt
    11461 aaatgttttg aaagctagcc aacatggcaa taaaaaatca tcaagtccag cctttctaga
    11521 aaaactcaaa ccagagctta ctcttatctc agttggaaag agcaatcgaa tgaaactccc
    11581 ccatcaggaa acattgacac gactggaagg tatcaatagc aaagtttatc gaactgacca
    11641 gcaaggagct atacgtttta aggggttgga tagttggaaa atcgaaagtg ttcgatagga
    11701 aggataaatg ttgtagatta gtgaaataaa ctaaaaattt gttgcataat aatgataaaa
    11761 acggcataat gaaaacgtat tcaatattga ggatataaaa tcattaaaaa tcagcaaaag
    11821 ttgttttagt tagttagttt ataatctatt ggtcttcttc agtccagtgt atctgctgtg
    11881 acagtcacta aaagttacaa gtatgattgg aatacggttt tggaatatag taccaactat
    11941 cacgaccatc agtatgcttg gattccggaa tggtctcgtt attacagcta ttctgagtat
    12001 aaagttggcg gaggctggaa ctacgctcgt tatgaggtca taaactacta tactggaggt
    12061 tattaatcct taaagagtga gaaaaggagg gctggatatg ttaaatctta ctcatgttac
    12121 cttaaaaacg cgacaagtca tcttgcaaga tgcggatttt acctttaaaa agggtaggat
    12181 ttatggcctt cttgctatca atggctcggg aaagacgaca ctattccgag ctatgagcaa
    12241 gttgcttccc cttagtagtg gacacatcgc agttcctcct tctttgtttt attatgagag
    12301 cgttgaatgg ctggatggaa acttaagtgg gatggactac cttcgtctca taaaaaacat
    12361 ctggaagtca gacctaaact tgagagatga aatcgcctac tgggaaatgg ctgactatat
    12421 cagtcttccc atccgcaagt attccttagg gatgaagcaa cgcttggtga ttgctatgta
    12481 ttttctcagt caggccaaat gctggctcat ggatgagatt acaaatggct tagacgagta
    12541 ttatcgacag aagttttttg ataggctagc acaaatcgat agacaagaac agctggttct
    12601 tttaagttcc cactataagg aagagttggt tgatatctgc gatagagtag taaccattca
    12661 tcaggggcag atagaagagg tttagtttat gaaagatgtt agtctatttt tattgaaaaa
    12721 agttttcaaa agtcgtttaa actggattat cttagcttta tttgtatctg tactcggtgt
    12781 taccttttat ttaaatagtc agactgcaaa ctcacacagc ttggagagca ggttggaaag
    12841 tcgcattgca gccaacgaga gggctatcaa tgaaaatgaa gagaaactct cccaaatgtc
    12901 tgataccagc tcggaggaat accagtttgc taaaaataat ttagacgtgc aaaaaaatct
    12961 tttgacgcga aagacagaaa ttctaacttt actaaaagaa gggcgctgga aagaagccta
    13021 ctatctgcag tggaaagatg aagagaagaa ttatgaattt gtatcaaatg acccgactgc
    13081 tagctctggc ttaaaaatgg gggttgaccg cgaacggaag atttaccaag ccctgtatcc
    13141 cttgaacata aaagcacata ctttggagtt tccgacccac gggattgatc agattgtctg
    13201 gattttagag gttatcatcc caagcttgtt tgtggttgcg attattttta tgctaacaca
    13261 actatttgca gaaagatatc aaaatcatct ggacacagct cacttatatc ctgtttcaaa
    13321 agtgacattt gcaatatcct ctcttggagt tggagtggga tatgtaactg tgctgtttat
    13381 cggaatctgt ggcttttctt ttctagtggg aagtctgata agtggttttg gacagttaga
    13441 ttatccctac ccaatttata gcttagtgaa tcaagaagta actattggga aaatacaaga
    13501 tgtattattt cctggcttgc tcttagcttt cttagccttt atcgtcattg tggaagttgt
    13561 gtacttgatt gcttactttt tcaagcaaaa aatgcctgtc ctctttcttt cactcattgg
    13621 gattgttggc ttattgtttg gcatccaaac aattcagcct cttcaaagga ttgcacatct
    13681 gattcccttt acttacttgc gttcagtgga gattttatct ggaagattac ctaagcagat
    13741 tgataatgtc gatctaaatt ggagcatggg aatggtctta cttccttgcc tgattatctt
    13801 tttgctattg ggaattctat ttattgaaag atggggaagt tcacagaaaa aaagaatttt
    13861 ttaatagatt ctagctttcc tataggtagg gaaaataagt aaaaactaac atagagaggg
    13921 aatcaacttg attctctctt tttgattcga aaaccaaacc aaaatacaaa cacaaacttt
    13981 tcaaaaaata actttttatc ttgacaagag ctagaaaact tggtatcata taaaagttga
    14041 gaaaagcaga agtgagagct tctcgccttg tgacattaag ttgcctggcc ctacggatga
    14101 aaagtttcga agaaacgcta tcataacgtg cgggcttgta tatttacgag tccgctattg
    14161 tttttctcta ataaaacaaa agaggtgaaa accatagcaa agcaagactt attcatcaat
    14221 gatgagattc gtgtacgtga agttcgcttg attggtcttg aaggagaaca gctaggcatc
    14281 aagccactca gtgaagcgca agctttggct gataacgcta atgttgacct agtattgatt
    14341 caaccccaag ccaaaccgcc tgttgcaaaa attatggact acggtaagtt caaatttgag
    14401 taccagaaga agcaaaaaga acaacgtaaa aaacaaagcg ttgttactgt gaaagaagtt
    14461 cgtctaagtc caactattga caagggtgac tttgatacaa aacttcgtaa tgcacgtaag
    14521 ttccttgaaa aaggaaataa agttaaggta tctattcgct ttaagggtcg tatgattacc
    14581 cataaagaga ttggtgcaaa agttttagcc gagtttgctg aagcaactca agatattgcc
    14641 atcatcgaac aacgtgctaa aatggatgga cgtcaaatgt tcatgcaatt ggcgccagcg
    14701 actgacaaaa aataattgtc agaaagttaa ataaaggaga aaaaattatg ccaaaacaaa
    14761 aaacacaccg cgcatcagct aaacgtttca aacgtacagg ttctggtgga cttaaacgtt
    14821 tccgtgctta cacttctcac cgtttccacg gaaaaactaa gaaacaacgt cgtcatcttc
    14881 gtaaagcatc tatggtgcat tcaggagatt acaaacgtat caaagcaatg cttactcgct
    14941 tgaaataata gcttgactgt aagtaaacaa ttctaggaaa tatttggagg aaataaaaca
    15001 tggcacgtgt taaaggtggc gttgtatcac gcaaacgtcg taaacgtatt cttaaattag
    15061 caaaaggtta ctatggagct aaacacatct tgttccgtac tgcaaaagaa caagtaatga
    15121 actcttacta ctatgcatac cgtgaccgtc gtcagaaaaa acgtgacttc cgcaaattgt
    15181 ggatcactcg tatcaatgcg gcagctcgta tgaacggact ttcatactca caattgatgc
    15241 atggtttgaa attggctgag atcgaagtta accgtaaaat gcttgctgac ttggctgtta
    15301 acgatgcagt agctttcaca gctcttgcag atgcagctaa agcaaaactt ggtaaataac
    15361 aactgatgag actggaacag gattgtccag tctttttaaa aataaaggag aaaaatatgg
    15421 cttcaaaaat gctacacact tgcttacgag tagaaaatct tgaaaaatca atcgcatttt
    15481 atcaagatgc ttttggtttt aaagaattgc gtcgcagaga ttttccagac catgccttca
    15541 cgattgtcta tctaggtctt gagggtgatg actatgagtt ggagttgact tataactacg
    15601 atcacggtcc gtatgtggtt ggtgatgggt ttgcccatat cgccctcagt acacctgatc
    15661 ttgaggcact tcatcaagaa cacagtgcaa aaggctatga agtgactgag ccaaatggtc
    15721 taccaggaac tacaccaaac tattactttg tcaaagaccc tgatggatat aaggtcgaag
    15781 tcattcgtga aaaataattt cgtgcaagtt aagtagaata ttcgtttttt acttgacttt
    15841 ttttagctga aagagtatac taataaaaat tcaaccttta aggggagtcc agagagactc
    15901 acaaggtgtc agataaaaga atagtgcaat tttctagagg agactttttg agtgtgctct
    15961 cttgtgttgt acgattttaa ctgaggcctt gcactagcaa ggtcttttct ttatctggtc
    16021 cccttaaaat ttaaggagga aaagttatga atcccacatg taagaagcgt ttgggtgtca
    16081 ttcggttgga aaccatgaag gtggttgcac aagaggaaat cgcgccagca atctttgaat
    16141 tagtcctaga aggagaaatg gttgaagcca tgcgagcagg ccaatttctt catctgcgtg
    16201 taccagacga tgcccatctt ttacgtcgtc ctatttcaat ttcgtctatt gacaaggcaa
    16261 acaagcagtg tcacctcatt tatcggattg agggagctgg gactgcaatt ttttcaacct
    16321 taagtcaggg agacactctt gatgtgatgg ggcctcaggg aaatggtttt gacttgtctg
    16381 accttgatga gcagaatcag gttctccttg ttggtggtgg gattggtgtt ccacccttgc
    16441 ttgaggtggc caaggaattg catgaacgtg gagtgaaagt agtgacagtc ctcggttttg
    16501 ctaataagga tgctgttatt ttgaaaacgg aattggctca gtatggtcag gtctttgtaa
    16561 cgacagatga tggttcttat ggcatcaagg gaaatgtttc cgttgttatc aatgatttag
    16621 atagtcagtt tgatgctgtt tactcgtgtg gggctccagg aatgatgaag tatatcaatc
    16681 aaacctttga tgatcaccca agagcctatt tatctctgga atctcgtatg gcttgtggga
    16741 tgggagcttg ctatgcctgt gttctaaaag taccagaaag cgagacggtc aaccaacgcg
    16801 tctgtgaaga tggtcctgtt ttccgcacag gaacagttgt attataagga gaaaattatg
    16861 actacaaatc gattacaagt gtctctacct ggtttggatt tgaaaaatcc gattattcca
    16921 gcatcaggtt gttttggctt tggacaagag tatgccaagt actatgattt aaacctttta
    16981 ggttctatta tgatcaaggc gacaaccctt gaaccacgtt ttgggaaccc aactccaaga
    17041 gtagcagaga cgcctgctgg tatgctcaat gcaattggct tgcaaaatcc tggtttagag
    17101 gttgttttgg ctgaaaagct accttggctg gaaagagaat atccaaatct tcctattatt
    17161 gccaatgtag ctggtttttc aaaacaagag tatgcagctg tttctcatgg gatttccaag
    17221 gcaactaatg taaaagctat cgagctcaat atttcttgtc ccaatgttga ccactgtaat
    17281 catggacttt tgattggtca agatccagat ttggcttatg atgtggtgaa agcagctgtg
    17341 gaagcctcag aagtgccagt ttatgtcaaa ttaaccccga gtgtgaccga tatcgttact
    17401 gtcgcaaaag ctgcagaaga tgcgggagca agtggcttga ccatgatcaa tactctggtt
    17461 ggaatgcgct ttgacctcaa aaccagaaaa ccaatcttgg ccaatggaac aggtggaatg
    17521 tcaggtccag cagtctttcc agtagccctc aaactcatcc gccaagtagc ccaaacaaca
    17581 gacctgccta tcattggaat ggggggagtg gattcggctg aagctgccct agaaatgtat
    17641 ctggctgggg catctgctat cggagttgga acagctaact ttaccaatcc ttatgcctgc
    17701 cctgacatca tcgaaaattt accaaaagtc atggataaat acggtattag cagtctggaa
    17761 gaactccgtc aggaagtaaa agagtctctg aggtaaactg caatcaatct gttcttgatt
    17821 ttttattagt ttgtaatatg aatttaggag aattttggta caataaaata aataagaaca
    17881 gaggaagaag gttaatgaag aaagtaagat ttattttttt agctctgcta tttttcttag
    17941 ctagtccaga gggtgcaatg gctagtgatg gtacttggca aggaaaacag tatctgaaag
    18001 aagatggcag tcaagcagcg aatgagtggg tttttgatac tcattatcaa tcttggttct
    18061 atataaaagc agatgctaac tatgctgaaa atgaatggct aaagcaaggt gacgactatt
    18121 tttacctcaa atctggtggc tatatggcca aatcagaatg ggtagaagac aagggagcct
    18181 tttattatct tgaccaagat ggaaagatga aaagaaatgc ttgggtagga acttcctatg
    18241 ttggtgcaac aggtgccaaa gtaatagaag actgggtcta tgattctcaa tacgatgctt
    18301 ggttttatat caaagcagat ggacagcacg cagagaaaga atggctccaa attaaaggga
    18361 aggactatta tttcaaatcc ggtggttatc tactaacaag tcagtggatt aatcaagctt
    18421 atgtgaatgc tagtggtgcc aaagtacagc aaggttggct ttttgacaaa caataccaat
    18481 cttggtttta cataaaagaa aatggaaact atgctgataa agaatggatt ttcgagaatg
    18541 gtcactatta ttatctaaaa tctggtggct acatggcagc caatgaatgg atttgggata
    18601 aggaatcttg gttttatctc aaatttgatg ggaaaatagc tgaaaaagaa tgggtctacg
    18661 attctcatag tcaagcctgg tactacttca aatccggtgg ttacatgaca gccaatgaat
    18721 ggatttggga taaggaatct tggttttacc tcaagtctga tggcaaaatg gctgaaaaag
    18781 aatgggtcta cgattctcat agtcaagcct ggtactactt caaatctggt ggttacatgg
    18841 cgaaaaatga gacagtagat ggttatcagc ttggaagcga tggtaaatgg cttggaggaa
    18901 aagctacaaa tgaaaatgct gcttactatc aagtagtgcc tgttacagcc aatgtttatg
    18961 attcagatgg tgaaaagctt tcctatatat cgcaaggtag tgtcgtatgg ctagataagg
    19021 atagaaaaag tgatgacaag cgcttggcta ttactatttc tggtttgtca ggctatatga
    19081 aaacagaaga tttacaagcg ctagatgcta gtaaggactt tatcccttat tatgagagtg
    19141 atggccaccg tttttatcac tatgtggctc agaatgctag tatcccagta gcttctcatc
    19201 tttctgatat ggaagtaggc aagaaatatt attcggcaga tggcctgcat tttgatggtt
    19261 ttaagcttga gaatcccttc cttttcaaag atttaacaga ggctacaaac tacagtgctg
    19321 aagaattgga taaggtattt agtttgctaa acattaacaa tagccttttg gagaacaagg
    19381 gcgctacttt taaggaagcc gaagaacatt accatatcaa tgctctttat ctccttgccc
    19441 atagtgccct agaaagtaac tggggaagaa gtaaaattgc caaagataag aataatttct
    19501 ttggcattac agcctatgat acgacccctt acctttctgc taagacattt gatgatgtgg
    19561 ataagggaat tttaggtgca accaagtgga ttaaggaaaa ttatatcgat aggggaagaa
    19621 ctttccttgg aaacaaggct tctggtatga atgtggaata tgcttcagac ccttattggg
    19681 gcgaaaaaat tgctagtgtg atgatgaaaa tcaatgagaa gctaggtggc aaagattagt
    19741 actataagtg aatatgattt gagtgaatag taagttaaaa atcctgattt caagtaaaat
    19801 caggattttt tcatggatgc aatttttttg gagtctggtg tgacgcggag ggtcttttgt
    19861 cctgtgtaag tgacaaagcc gggttttcca ccagttggtt tattgagttt tttgacttca
    19921 atcatatcta cctgcaccag attcgacagg cgcccttgag agaagtaggc agctaactct
    19981 gctgcgtctg tcttgactgc atcagatggg tcaagatttc ctgagatgac aacatggctt
    20041 ccaggaatgt ccttagcatg gaaccaaagt tcctccttgc gggccatttt aaaggtcaat
    20101 tcctcatttt gaagattgtt tcgtccgaca tagatgatgg ttttgccatc gcttgctaga
    20161 tattgttcta gttttttgcg tttctggatt ttctcccgtt gtcttctgcg gataaaacct
    20221 gtttgaatca attcttcacg gatttcagcg atttcttcca gtccagcttg gttgaggacg
    20281 gtttctacac tttccagata gagaatagtg gctttggttt cttcaatcaa atcagtcaag
    20341 tatttgacag cttctttgag tttctgatac cgtttaaaat agcgttgggc attctggttg
    20401 ggagtcagag ccttatcaag cgcaatcatg ataggttggt tggtatagta gttgtctagg
    20461 ataacctggt cttggtcgtt aggcacttgg tggaggaagg ttgtcagcaa ttctcctttt
    20521 tgacgaaatt cttcagcgtt gtctgtcgcc agtaactctt tttcctgttt tttgagtttg
    20581 tgtcggtttt tctgaagttc attttcaaca cgacgaatca gttcactggc ctgctgtttg
    20641 acgcggtcgc gctcagcctt atccttatag taggtgtcca acaaatcaga aagatttgca
    20701 agaggctctc ccacctgatt tgcaaaagga actggactga aggaagtctc agtcaagcat
    20761 ggcttggttt cttgattgaa aaaatttcgg aaagcggaaa gtttttcact aaccagtatc
    20821 ctttccaatt catttgccgt atcgcgtccc agaccttgaa agaggctttg aagatttttt
    20881 gctgttagtt cttgggtttg caggatttca aagagctttt catccttgat agtaaaagga
    20941 ttgagagatt ttgtacttgg cggagcgata taggtcgatc ctggaagtaa ggtgcggtag
    21001 ctattttgtg aaaagccgac gtgtttgata acttcgagga ttttatgact gcttttatcg
    21061 accagtagaa tattactgtg tttccccata atttcgataa tcaaggtagc ctggatatgg
    21121 tctccaatct cgtttttatt ggaaactgta atttccacaa tacggtcatt ttccacttgc
    21181 tcaatcgact caatcagggc cccctgcaaa tactttctca aaaccatgat aaaggtagaa
    21241 ggttgggctg gattttcaaa agtcgtttgg gtcagctgaa tgcgtccaaa aactggatgg
    21301 gcagaaagga gcaggcgatg gctttggcga ttgctgcgga tttgcaagac caactcttgt
    21361 tcaaaaggct gattgatttt ctggatgcga ccattcacta attcgcttcg caattcctca
    21421 actatgtggt gtaaaaaaaa tccgtcaaat gacatcgttc tctccttgtg attgtattcc
    21481 atagtattat atcaaaaagg tagaataaaa tcatggaaat gtggtataat aaagccaagt
    21541 aaagagaaac gagaagcaca tgtatattga aatggtagat gaaactggtc aagtttcaaa
    21601 agaaatgttg caacaaaccc aagaaatttt ggaatttgca gcccaaaaat taggaaaaga
    21661 agacaaggag atggcagtca cttttgtgac caatgagcgt agtcatgaac ttaatctggg
    21721 gtaccgtgac accgaccgtc cgacagatgt catcagcctt gagtataaac cagaattgga
    21781 aattgccttt gacgaagagg atttgcttga aaatccagaa ttggcagaga tgatgtctga
    21841 gtttgatgcc tatattgggg aattgttcat ctctatcgat aaggctcatg agcaggccga
    21901 agaatatggt cacagctttg agcgtgagat gggcttcttg gcagtacacg gctttttaca
    21961 tattaacggc tatgatcact atactccgga agaagaagcg gagatgttcg gtttacaaga
    22021 agaaattttg acagcctatg gactcacaag acaataaacg aaaatggaaa aatcgtgacc
    22081 tgatatccag tttagaattt gccatcacag ggatttttac tgctatcaag gaagaacgca
    22141 atatgcgaaa acatgcagtg acggctctag tggtcatcct tgcaggtttt ggttttcagg
    22201 tgtcacgaat cgaatggctc tttctcctat tgagcatttt cttggtggta gcctttgaaa
    22261 ttatcaattc tgctatcgaa aatgtggtgg atttggccag tcactatcac ttttccatgc
    22321 tggctaaaaa tgccaaggat atggcggctg gcgcggtatt agtggtttct ctttttgcag
    22381 ccttaacagg cgcattgatt tttctcccac gaatctggga tttattattt taaacagtaa
    22441 gaggaaatta tgacttttaa atcaggcttt gtagccattt taggacgtcc caatgttggg
    22501 aagtcaacct ttttaaatca cgttatgggg caaaagattg ccatcatgag tgacaaggcg
    22561 cagacaacgc gcaataaaat catgggaatt tacacgactg ataaggagca aattgtcttt
    22621 atcgacacac cagggattca caaacctaaa acagctctcg gagatttcat ggttgagtct
    22681 gcctacagta cccttcgcga agtggacact gttcttttca tggtgcctgc tgatgaagcg
    22741 cgtggtaagg gggacgatat gattatcgag cgtctcaagg ctgccaaggt tcctgtgatt
    22801 ttggtggtga ataaaatcga taaggtccat ccagaccagc tcttgtctca gattgatgac
    22861 ttccgtaatc aaatggactt taaggaaatt gttccaatct cagcccttca gggaaataac
    22921 gtgtctcgtc tagtggatat tttgagtgaa aatctggatg aaggtttcca atatttcccg
    22981 tctgatcaaa tcacagacca tccagaccgt ttcttagttt cagaaatggt tcgcgagaaa
    23041 gtcttgcacc taactcgtga agagattccg cattctgtag cagtagttgt tgactctatg
    23101 aaacgagacg aagagacaga caaggttcac atccgtgcaa ccatcatggt cgagcgcgat
    23161 agccaaaaag ggattatcat cggtaaaggt ggcgctatgc ttaagaaaat cggtagcatg
    23221 gcccgtcgtg atatcgaact catgctagga gacaaggtct tcctagaaac ctgggtcaag
    23281 gtcaagaaaa actggcgcga taaaaagcta gatttggctg actttggcta taatgaaaga
    23341 gaatactaag tagaggtggg ctcatgcctg cttcttgttt ttacagaagg aggacttatg
    23401 cctgaattac ctgaggttga aaccgtttgt cgttgcttag aaaaattgat tataggaaag
    23461 aagatttcga gtatagaaat tcgctacccc aagatgatta agacggattt ggaagagttt
    23521 caaagggaat tgcctagtca gattatcgag tcaatgggac gtcgtggaaa atatttgctt
    23581 ttttatctga cagacaaggt tttgatttcc catttgcgga tggagggcaa gtatttttac
    23641 tatccagacc aaggacctga acgcaagcat gcccatgttt tctttcattt tgaagatggt
    23701 ggcacgcttg tttatgagga tgttcgcaag tttggaacca tggaactctt ggtgcctgac
    23761 cttttagacg cctactttat ttctaaaaaa ttaggtcctg aaccaagcga acaagacttt
    23821 gatttacagg tctttcaatc tgcccttgcc aagtccaaaa agcctatcaa atcccatctc
    23881 ctagaccaga ccttggtagc tggacttggc aatatctatg tggatgaggt tctctggcga
    23941 gctcaggttc atccagctag atcttcccag actttgacag cagaagaagc gacagctatt
    24001 catgaccaaa ccattgctgt tttgagccag gctgttgaaa aaggcggttc caccattcgg
    24061 acctatacca atgcctttgg ggaagatgga agcatgcagg attttcatca ggtctatgat
    24121 aagactggtc aaggatgtgt acgctgtggt accatcattg agaaaattca actaggtgga
    24181 cgtggaaccc acttttgtcc aaactgtcaa aggagggact gatgggaaaa atcatcggaa
    24241 tcactggggg aattgcctct ggtaagtcaa ctgtgacaaa ttttctaaga cagcaaggct
    24301 ttcaagtagt ggatgccgac gcagtcgtcc accaactaca gaaacctggt ggtcgtctgt
    24361 ttgaggctct agtacagcac ttttggcaag aaatcattct tgaaaacgga gaactcaatc
    24421 gccctctcct agctagtctc atcttttcaa atcctgatga acgagaatgg tctaagcaaa
    24481 ttcaagggga gattatccgt gaggaactgg ctactttgag agaacagttg gctcagacag
    24541 aagagatttt cttcatggat attcccctac tttttgagca ggactacagc gattggtttg
    24601 ctgagacttg gttggtctat gtggaccgag atgcccaagt agaacgctta atgaaaaggg
    24661 accagttgtc caaagatgaa gctgagtctc gtctggcagc ccagtggcct ttagaaaaaa
    24721 agaaagattt ggccagccag gttcttgata ataatggcaa tcagaaccag cttcttaatc
    24781 aagtgcatat ccttcttgag ggaggtaggc aagatgacag agattaactg gaaggataat
    24841 ctgcgcattg cctggtttgg taattttctg acaggagcca gtatttcttt ggttgtacct
    24901 tttatgccta tcttcgtgga aaatctaggt gtagggagtg agcaagtcgc tttttatgca
    24961 ggcttagcaa tttctgtctc tgctatttcc gcggcgctct tttctcctat ttggggtatt
    25021 cttgctgaca aatacggccg aaaacccatg atgattcggg caggtcttgc tatgactatc
    25081 actatgggag gcttggcctt tgtcccaaat atctattggt taatctttct tcgtttacta
    25141 aacggtgtat ttgcaggttt tgttcctaat gcaacggcac tgatagccag tcaggttcca
    25201 aaggagaaat caggctctgc cttaggtact ttgtctacag gcgtagttgc aggtactcta
    25261 actggtccct ttattggtgg ctttatcgca gaattatttg gcattcgtac agttttctta
    25321 ctggttggta gttttctatt tttagctgct attttgacta tttgctttat caaggaagat
    25381 tttcaaccag tagccaagga aaaggctatt ccaacaaagg aattatttac ctcggttaaa
    25441 tatccctatc ttttgctcaa tctcttttta accagttttg tcatccaatt ttcagctcaa
    25501 tcgattggcc ctattttggc tctttatgta cgcgatttag ggcagacaga gaatcttctt
    25561 tttgtctctg gtttgattgt gtccagtatg ggcttttcca gcatgatgag tgcaggagtc
    25621 atgggcaagc taggtgacaa ggtgggcaat catcgtctct tggttgtcgc ccagttttat
    25681 tcagtcatca tctatctcct ctgtgccaat gcctctagcc cccttcaact aggactctat
    25741 cgtttcctct ttggattggg aaccggtgcc ttgattcccg gggttaatgc cctactcagc
    25801 aaaatgactc ccaaagccgg catttcgagg gtctttgcct tcaatcaggt attcttttat
    25861 ctgggaggtg ttgttggtcc catggcaggt tctgcagtag caggtcaatt tggctaccat
    25921 gctgtctttt atgcgacgag cctttgtgtt gcctttagtt gtctctttaa cctgattcaa
    25981 tttcgaacat tattaaaagt aaaggaaatc tagtgcgagt aaaaattaat ctcaaatgct
    26041 cctcttgtgg cagtatcaat tacctaacca gtaaaaattc aaaaacccat ccagacaaga
    26101 ttgaggtttt aaagtattgt cccaaggaaa gaaaagtaac cctacatctt gaatctaagt
    26161 agattttatg gtaaaataat agggatttta aggagtttga tatgtataac ctattattaa
    26221 ccattttatt agtattatct gttgtgattg tgattgcaat tttcatgcaa ccaaccaaaa
    26281 accaatccag caatgtattt gatgccagtt caggtgattt gtttgaacgt agtaaagctc
    26341 gcggttttga agctgtaatg cagcgtttga cagggatttt agtctttttc tggctagcca
    26401 ttgccttagc attgacggta ttatcaagta gataagaaaa taatgggcag gactaggtct
    26461 ttgcctcttt ttatttttaa aggatgtttg agaaggtttt acagtaaaag aaaattaaaa
    26521 aatctagaaa gaaaatatga aagatagaat aaaagaatat ttacaagaca agggaaaggt
    26581 gactgttaat gatttggctc aggctttggg aaaagacagt tccaaggatt ttcgtgagtt
    26641 gattaaaacc ttgtccttaa tggaaagaaa gcaccaaatt cgttttgaag aagatggtag
    26701 tctgacatta gaaattaaga aaaaacatga gattaccctc aaggggattt ttcatgccca
    26761 taaaaatggc tttggctttg ttagtctgga aggcgaggag gacgaccttt ttgtagggaa
    26821 aaatgatgtc aactatgcta ttgatggtga taccgtcgag gtagtgatta agaaagtcgc
    26881 tgaccgcaat aagggaacag cagcagaagc caaaattatt gatatcctag aacacagttt
    26941 gacaacagtt gtcgggcaaa tcgttctgga tcaggaaaaa cctaagtatg ctggctatat
    27001 tcgttcaaaa aatcagaaaa tcagtcaacc gatttatgtt aagaaaccag ccctaaaatt
    27061 agaaggaaca gaagttctca aagtctttat cgataaatac ccaagcaaga aacatgattt
    27121 ctttgtcgcg agtgttctcg atgtagtggg acactcaacg gatgtcggaa ttgatgttct
    27181 tgaggtcttg gaatcaatgg acattgtatc cgagtttcca gaagctgttg ttaaggaagc
    27241 agaaagtgtg cctgatgctc cgtctcaaaa ggatatggaa ggtcgtctgg atctaagaga
    27301 tgaaattacc tttaccattg acggtgcgga tgccaaggac ttggacgatg cagtgcatat
    27361 caaggctctg aaaaatggca atctggagct tggggttcac atcgcagatg tttcttatta
    27421 tgtgaccgag gggtctgccc ttgacaagga agcccttaac cgtgcgactt ctgtttacgt
    27481 gacagaccga gtggtgccaa tgcttccaga acgactatca aatggcatct gctctctcaa
    27541 tccccaagtt gaccgcctga cccagtctgc tattatggag attgataaac atggtcgtgt
    27601 ggtcaactat accattacac aaacagttat caagaccagt tttcgtatga cctatagcga
    27661 tgtcaatgat atcctagctg gcgatgaaga aaagagaaaa gaatatcata aaattgtatc
    27721 aagtatcgaa ctcatggcca agcttcatga aactttagaa aacatgcgtg tgaaacgtgg
    27781 agctctcaat tttgatacca atgaagcgaa gattttagtg gataaacaag gtaagcctgt
    27841 tgatatcgtt cttcggcagc gtggtattgc cgagcggatg attgagtctt ttatgttgat
    27901 ggctaatgaa acagttgccg aacatttcag caagttggat ttgcctttta tctatcgaat
    27961 tcacgaggag cctaaggctg aaaaggttca gaagtttatt gattatgctt cgagttttgg
    28021 cttgcgcatt tatggaactg ccagtgagat tagtcaggag gcacttcaag acatcatgcg
    28081 tgctgttgag ggagaacctt atgcagatgt attgtccatg atgcttcttc gctctatgca
    28141 gcaggctcgt tattcggagc acaatcacgg ccactatgga ctagctgctg actattatac
    28201 tcactttacc agtccaattc gtcgttatcc agaccttctt gttcaccgta tgattcggga
    28261 ttacggccgt tctaaggaaa tagcagagca ttttgaacaa gtgattccag agattgcgac
    28321 ccagtcttcc aaccgtgaac gtcgtgccat agaagctgag cgtgaagtcg aagccatgaa
    28381 aaaggctgag tatatggaag aatacgtggg tgaagagtat gatgcagttg tatcaagtat
    28441 tgtcaaattc ggtctctttg tcgaattgcc aaacacagtt gaaggcttga ttcacatcac
    28501 taatctacct gaattttatc atttcaatga gcgtgatttg actcttcgtg gagaaaaatc
    28561 aggtatcact ttccgagtgg gtcagcagat ccgtatccgt gttgaaagag cggataaaat
    28621 gactggagag attgattttt cattcgtacc tagtgagttt gatgtgattg aaaaaggctt
    28681 gaaacagtct agtcatagtg gcagagggcg tggttcaaat cgtcgttcgg ataagaagga
    28741 agacaagaga aaatcaggac gctcaaatga taagcgtaag cattcacaaa aagacaagaa
    28801 gaaaaaagga aagaaacctt tttacaagga agtagctaag aaaggagcca agcatggcaa
    28861 agggcgaggg aaaggtcgtc gcacaaaata aaaaggcacg ccacgactat acaatcgtag
    28921 atacgctaga ggcagggatg gtcctgactg gaactgaaat caagagtgta cgagctgctc
    28981 gaattaatct caaggatggc tttgctcaag tgaaaaatgg agaagtttgg ctgagcaatg
    29041 ttcatatcgc gccttacgaa gagggcaata tctggaacca ggaaccagaa cgtcgtcgta
    29101 aactcctgct ccataaaaag caaattcaaa aattggaaca agagaccaaa gggacaggaa
    29161 tgaccttagt tccccttaag gtctatataa aagatggcta cgctaagctt cttttaggac
    29221 ttgccaaagg gaagcatgac tatgacaaac gggagtctat caaacgtcgt gagcaaaatc
    29281 gagatatcgc gcgtgtgatg aaagctgtta atcagcgata aaaagaggaa ttgaaaatgg
    29341 aaaaattagt tgcctataaa cgcatgcctt tgtggaataa acaaacaatg cctgaagatg
    29401 ttcagcaaaa gcacaataca aaagttggga cttgggggaa aattactgtc ttgaagggag
    29461 ctctcaagtt tattgaattg acagaagaag gggaagttct agctgaacac ctctttgaag
    29521 caggggcaga caatccaatg gcccaacctc aagcctggca ccgagtggaa gctgccacag
    29581 atgatgtgga atggtacttg gaattttatt gtaaacctga ggattatttt gctaaaaaat
    29641 acaataccaa tcctgttcat tcagaggtcc tagaggccat gcagacagtg aaacaaggga
    29701 aagctttgga tttgggttgt ggtcaggggc gtaattctct ttttctagcc cagcaagatt
    29761 ttgatgtgac ggctgtagat caaaatggac tagctcttga aatcttgcaa agcattgtgg
    29821 agcaggaaga tttggacatg cctgttggcc tttacgatat caattcagct agcattgaac
    29881 aagaatatga ttttatcgtt tcaacagttg ttctcatgtt tctacaagcg gaccgcattc
    29941 cagctattat tcaaaatatg caggagaaaa ccagtgttgg tggttacaac cttatcgttt
    30001 gtgccatgga cacggaggat tatccttgct cggttaattt cccattcacc tttaaagaag
    30061 gagaactggc agactattac aaggattggg aattggttaa gtacaatgaa aatccaggcc
    30121 atttgcaccg tcgcgatgag aatggcaatc gtattcaact acgctttgcg accttactag
    30181 ctaagaaaat caagtaaaca cacatgaaga ttaggaattt tcctgatctt ttttcttttt
    30241 tacgaatgat atagaaaagg agggaattca tgtttgttgc gagagatgct aggggagaat
    30301 tggtaaatgt gttagaggat aaacttgaga agcaagcata cacctgccca gcttgtggag
    30361 gccagctcca tttgcgtcaa ggaccaagtg tacggacgca ttttgcccat aaatccttaa
    30421 aagactgtga ttttttcttt gaaaatgaaa gtccagaaca cctggccaat aaggaatccc
    30481 tctatcactg gttgaaaaaa gagacaaagg ttcaattaga gtacccgctt tcagaactta
    30541 aacagattgc ggatgtattt gtaaatggca atctagctct agaagttcag tgtagtccct
    30601 tgcctcagaa agtccttaaa gagcgaagtg agggatatcg tagtcagggt taccaagtac
    30661 tgtggttgct gggtcaaaaa ctgtggctca aggagcgttt gactcgtcta cagcaaggtt
    30721 ttctttattt cagtcaaaac atgggctttt atgtttggga attagacaag gaaaaacaag
    30781 ttttaagact caaatacctg atttaccagg atctccgcgg taaactccat tatcaaatca
    30841 aggaattttc ctatggtcaa ggta
//
LOCUS       DAVBCB010000022        30074 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVBCB010000022 DAVBCB010000000
VERSION     DAVBCB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30074
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.022"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..1100
                     /locus_tag="VQU28_000966"
     CDS             51..1100
                     /locus_tag="VQU28_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HET2476336.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAVHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQU28_000967"
     CDS             complement(1200..5042)
                     /gene="spuA"
                     /locus_tag="VQU28_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HET2476337.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPGNANASLEKTEEKPAASPA
                     DPAPQTGQDRSSEPTTSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDD
                     VEKPSENWPNGALSFKDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVE
                     KLVPKMNEAWLDQDYKVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQW
                     PDGTDFTATGKYGRYIDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQ
                     IFLKDDDESIYTNPYYVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHL
                     GNKVTITDVAIDEAGKKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGK
                     LGADLKEEGKQVDLTLWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDST
                     NKLGITDFTGYYYQYQIERQGKTVLALDPYAKSLAAWNSDDSKIDDAHKVAKAAFVDP
                     AKLGPQDLTYGKIHNFKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDY
                     LKDLGVTHIQLLPVLSYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSD
                     PKNPEKRIAEFKNLINEIHKRGMGAILDVVYNHTAKVDLFEDLEPNYYHFMDADGTPR
                     TSFGGGRLGTTHHMTKRLLIDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARAL
                     NPNLIMLGEGWRTYAGDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPA
                     FITGGKRDVNTIFKNLIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKA
                     ENYAEIHRRLRLGNLMVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHL
                     LRDKDGNPFDYPYFIHDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQS
                     TDAFRLKSLQDIKDRVHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKARE
                     FNLGTAFAHLRNAEVLADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTS
                     HESTAEEKPDSTPSKPEHQNEASHPEHQDPAPEARPDSTKPDTKVADAENKPSQATAD
                     SQAEQPAQEAQASSVKEAVRNESVENSSKKNIPATPDRQAELPNTGIKNENKLLFAGI
                     SLLALLGLGFLLKNKKEN"
     gene            5459..5872
                     /gene="rpsL"
                     /locus_tag="VQU28_000968"
     CDS             5459..5872
                     /gene="rpsL"
                     /locus_tag="VQU28_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HET2476338.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            5892..6362
                     /gene="rpsG"
                     /locus_tag="VQU28_000969"
     CDS             5892..6362
                     /gene="rpsG"
                     /locus_tag="VQU28_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HET2476339.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            6787..8868
                     /gene="fusA"
                     /locus_tag="VQU28_000970"
     CDS             6787..8868
                     /gene="fusA"
                     /locus_tag="VQU28_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HET2476340.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEEIRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            8972..13363
                     /locus_tag="VQU28_000971"
     CDS             8972..13363
                     /locus_tag="VQU28_000971"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HET2476341.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMAPPPAEEKPAFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFESVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYRSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            13465..13728
                     /locus_tag="VQU28_000972"
     CDS             13465..13728
                     /locus_tag="VQU28_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HET2476342.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            13721..13999
                     /locus_tag="VQU28_000973"
     CDS             13721..13999
                     /locus_tag="VQU28_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HET2476343.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            14020..14172
                     /locus_tag="VQU28_000974"
     CDS             14020..14172
                     /locus_tag="VQU28_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476344.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            14194..15435
                     /locus_tag="VQU28_000975"
     CDS             14194..15435
                     /locus_tag="VQU28_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HET2476345.1"
                     /translation="MVLPNFKENLGKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEEFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFKNAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            15445..15675
                     /locus_tag="VQU28_000976"
     CDS             15445..15675
                     /locus_tag="VQU28_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HET2476346.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAILGAMIVLAIFWRRGS"
     gene            15898..16620
                     /locus_tag="VQU28_000977"
     CDS             15898..16620
                     /locus_tag="VQU28_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HET2476347.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNNGQLAHVLLSPKRHVDKTYQAQVKGIMTQEDVETFIKGIPLKDFTC
                     QPARLEILSTDAEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            16837..18171
                     /gene="pepC"
                     /locus_tag="VQU28_000978"
     CDS             16837..18171
                     /gene="pepC"
                     /locus_tag="VQU28_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HET2476348.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(18227..19138)
                     /locus_tag="VQU28_000979"
     CDS             complement(18227..19138)
                     /locus_tag="VQU28_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2476349.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(19162..19965)
                     /locus_tag="VQU28_000980"
     CDS             complement(19162..19965)
                     /locus_tag="VQU28_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HET2476350.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(19993..20982)
                     /gene="manX"
                     /locus_tag="VQU28_000981"
     CDS             complement(19993..20982)
                     /gene="manX"
                     /locus_tag="VQU28_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HET2476351.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(21215..22234)
                     /gene="adhP"
                     /locus_tag="VQU28_000982"
     CDS             complement(21215..22234)
                     /gene="adhP"
                     /locus_tag="VQU28_000982"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HET2476352.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLTLAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(22573..23385)
                     /locus_tag="VQU28_000983"
     CDS             complement(22573..23385)
                     /locus_tag="VQU28_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2476353.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            23575..24993
                     /locus_tag="VQU28_000984"
     CDS             23575..24993
                     /locus_tag="VQU28_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HET2476354.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            25046..25753
                     /locus_tag="VQU28_000985"
     CDS             25046..25753
                     /locus_tag="VQU28_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2476355.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGLSIVVLALFIMGARKTKLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            25848..26822
                     /gene="folP"
                     /locus_tag="VQU28_000986"
     CDS             25848..26822
                     /gene="folP"
                     /locus_tag="VQU28_000986"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HET2476356.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGANLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGITPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            26824..28146
                     /locus_tag="VQU28_000987"
     CDS             26824..28146
                     /locus_tag="VQU28_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2476357.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGIPLVTGRIAPEALTVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            28127..28681
                     /gene="folE"
                     /locus_tag="VQU28_000988"
     CDS             28127..28681
                     /gene="folE"
                     /locus_tag="VQU28_000988"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HET2476358.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            28724..29536
                     /gene="folK"
                     /locus_tag="VQU28_000989"
     CDS             28724..29536
                     /gene="folK"
                     /locus_tag="VQU28_000989"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HET2476359.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFEFYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPTLKQPIRNLYDALKK"
     gene            complement(29581..29898)
                     /locus_tag="VQU28_000990"
     CDS             complement(29581..29898)
                     /locus_tag="VQU28_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HET2476360.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMYGRETILLPEEN
                     DLF"
BASE COUNT         8527 a         5841 c         7006 g         8700 t
ORIGIN      
        1 ttagaataat ttttcaaatt gaaggacaga gatagacaag gagaatcaca atggtagaat
       61 tgggaatttc aacatttggg gaaataacgg agcttgaagg gactggacaa acttacagtc
      121 atgctgaacg cattcgccag ttggtggcag agattgagct ggctgacaag gttggtttgg
      181 acgtatatgg gattggcgag caccatcggg cggattttgc agtatcagcc ccagagattg
      241 tcctggccgc tggggcagtc aataccaaga aaatccgttt gaccagtgca gtcagcattc
      301 tgtcaagcat ggatccgatt cgtttgttcc aacagtatgc cactatcgat gctttgtcaa
      361 atggacgagc ggagattatg gctggaaggg gctctttcac ggaatctttc cctctgtttg
      421 gttatgactt gaaagactac gactctcttt ttgatgagaa attagacttg cttcagttag
      481 tcaatgaaaa gaccaagtta gactggcaag gtcgattgac tcaaacgatt gctggtaaag
      541 aagtttatcc tcgtccagtt caggacaaat tgcccttgtg gatagctaca ggtggtcatg
      601 tcgaatcaac agtgaagatt gctcaggctg gtctaccgat tgtatatgct attattggtg
      661 gtaatccacg ttattttaaa aagttgattc aggcttatcg tgagattgga agcgaagcgg
      721 tccatgctga caaagacttg aaagtgggag cccattcttg ggggtggatt gcggaagatg
      781 gtgagcaggc ggtgaaagat tatttccatc cgactaagca agtggtggat gctatttcca
      841 aagaccgtcc gcactggcag gaattgcgtt atgagcaata tttggagcag gtagggtcaa
      901 atggcgccat gtttgttggc aatccagatc aggtggccga aaaattgatt cgcatgatag
      961 aggatttagg attggaccgc ttcatgcttc atctaccgct tggttctatg cctcatgacc
     1021 aggttctgag agctattgaa ctcttcggaa cacaagttgc acccaaagta cgagcttatt
     1081 ttgctatgaa agaggcttaa taaaaaatcc taatcaagtt tattaggaca acaaatattg
     1141 tacgaaaatt aatcccccga gctataatgc ttgggggatt tttctatagg agggctagtt
     1201 tagttctctt ttttattttt tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt
     1261 cctgcaaata ggagtttgtt ttcgttttta attcctgtat ttggaagttc agcttgtcta
     1321 tctggggttg caggtatatt tttcttgcta gagttttcta ccgattcgtt tcgaaccgct
     1381 tcttttacag atgatgcttg tgcttcttgt gctggttgtt cagcttgtga atcagctgta
     1441 gcttggctag gtttattttc cgcatcagct actttggtat ctggtttagt agaatcaggt
     1501 ctagcttctg gagctggatc ttgatgttca gggtgagaag cttcattttg atgttcaggc
     1561 ttggaagggg ttgagtctgg tttctcttct gcagttgact catggctagt tccattttga
     1621 gagactcgaa gaacagtagc tgtaagggca ttcaatttca agcctttttc agtccattca
     1681 agtcctttcg ggttggcaat tccgactgat cctgcttggt tttcatctgc caaaacttcc
     1741 gcatttctta gatgtgcaaa ggcagttccc aaattaaatt cgcgagcttt ttcatccgca
     1801 ttgacaaaga ctgcgtagat atcgccgttt ggagcagtga tttgatagcc aatcactaca
     1861 tcctcttttt ccacaccatt ttggcctggg acagtgatga ggtggacacg gtctttgata
     1921 tcttgaagac tcttaagtcg gaaggcatct gtagattgac gaagggcaat caaacctttc
     1981 atatagtcac ggctcttgac attttcagga taagctttac catctgtagc cttagtccag
     2041 tcaaacttgt tgactgcatc actagaatcg taagagtcat ggatgaagta aggatagtca
     2101 aatgggttgc cgtccttatc acgcaacaag tgagatttgt ttggaacctt atcctctgct
     2161 actggagtct tgtaggctgg gtcacggaat tgtttagtac gtccatattc ctgaccggag
     2221 tggataaatg gagttccttg agctgtcaag accatgagat ttccaagtcg taaacgacgg
     2281 tggatttcag catagttctc agccttgctt gggtcttttt tgatagactg ggcaatgatg
     2341 tcaaagaggg tcaagttatc atgggctgcg atgtattgga tgacatctcc agggctgtca
     2401 gcttcaaagt tagttggttg agcaatgaga tttttaaaga tggtgttgac atcacgcttg
     2461 ccacctgtga taaaggcagg ttgaccttcg tttggataac cagatttgag gttgttacgg
     2521 atgtcatctg aaaagacagc gacagtatcg gtatgtttca tccaatcttg gtcagcagct
     2581 ttagtaggca tgttttcatc accggcatag gttctccaac cttcaccaag catgatgagg
     2641 tttggattga gggcgcgtgc agccttgtaa gcttcttcga tagaagcggc gtcatggtct
     2701 cccatcatat cgaaacggaa gccatccact ttgtaggtat caactaggta tttgatagag
     2761 tcaattagga gccgtttggt catatggtgg gttgtcccca agcgtccacc accaaagcta
     2821 gttcgaggtg tgccatcggc atccataaag tggtagtagt ttggttccaa atcttcaaag
     2881 agatcgactt tggctgtgtg gttataaacg acatctagga tagctcccat accacgtttg
     2941 tggatttcgt tgatgaggtt tttaaattct gcgattcgtt tttctggatt cttaggatcg
     3001 cttgagtaca taccagtcaa ggagaagtag ttttgagggt catatcccca gttgtagttg
     3061 ctgttgcttg aagcgtagtc agacaagcgt tcatggtttt tcaattcatt gacaaagtag
     3121 taagacaaga ctggaaggag ctggatatgg gttacaccca agtctttgag atagtctagt
     3181 ttttcaatga aggcttcaaa agtcccaaat ggtttggtca agtcttttgc aatggcagga
     3241 tctgaagtga aatcacgcac atgagcttcg tagataacgg cgtcttcacg agtcttgaaa
     3301 ttgtgaatct taccataagt caagtcttga ggtccgagtt tagctggatc tacaaaggcg
     3361 gctttagcca ctttatgggc atcgtcaatc ttggaatcgt cgctattcca agcagcaaga
     3421 gatttagcgt aaggatcgag tgcaagaaca gttttacctt gacgctcgat ttggtattga
     3481 taatagtagc cagtgaaatc tgtgattccg agtttgtttg tgctgtctag agtttgtttc
     3541 caagttcctc tttccccttt ttcaagagcg acagttccaa ctactttgtc agggtcattc
     3601 ttgtcgtaga caacaacaga aaccttatca gcacttggtg accaaagggt caaatcaact
     3661 tgttttcctt cttcttttag gtcagctccc agtttgccat catagctgta tgtctcatct
     3721 ttcaggcgcc agcttgtttt ggtagtgaat tggtcggaat tgtagctaac agtataagga
     3781 tgttttgtgt cagagaaatc tccgctgtag gtcactttct taccagcttc atcgattgca
     3841 acatcggtaa tagttacctt gtttcctagg tgattagtga tgttggagtg tttgaggata
     3901 tcttcttttt tagcaccgac aagtgttgaa aagctacttt caatgctaga agtgcctacg
     3961 tgttgggctc ctgtcatacg gatatcatgg acatagtatg gatttgtgta aatcgattca
     4021 tcatcgtctt ttaggaaaat ttggctatga tttttcaaat ctgtgaactt ataattttct
     4081 ttacggattt tcacgtcgtc tccttgtttg ctctcatcta gtaataaaaa tccaaattct
     4141 cttgcggctt cattaagagg aatgtcgata tagcggccat atttgcctgt agccgtaaag
     4201 tctgttccgt caggccattg agcgctactt ggatttttca catctcccca gtaccagaga
     4261 gatttcttgt catagttgcc atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc
     4321 tgtggctcgt aagagaaaac cttgtaatct tggtctaacc aagcttcgtt catttttgga
     4381 actagttttt ctacagattt atcgccggtt agattttttc cagctgtatt gttgatgagg
     4441 aagctaattt tcttggcttg ttctcccttt aatttgacat ctaggtaata gccgtagtca
     4501 tctttcttgg catccttgaa ggacaaagct ccgtttggcc agttttcaga tggtttttca
     4561 acatcgtccc aagtccatag tccttgagca tccttgtttt cttcaggaag ttttttgaca
     4621 tggatacgga agtagttatc ttcgatgggc tcttctgcct tagtttcagt tgttactgga
     4681 ctagtagaag tagttggctc acttgaacga tcttgtccag tttgtggtgc tggatcggct
     4741 gggcttgcag caggtttttc ttctgttttc tctagggaag cgtttgcatt tccaggtgaa
     4801 gaaatatcac tttcgttctt accagagcca gctgctgtat cagtaagagg ttgggcaagg
     4861 gtggttgtat ttctgttgtt gggttcgtta gtggttggtg tactttcgtc ggatgagata
     4921 gttggtgtag ccatggcaag caagacaagg cttgcgccga taaggacaga accagttcca
     4981 tttttgaggg aacggatgct gtagaccatt tttttctcag tgtgagatgg tgtttttctc
     5041 ataatgattc tccaatcaat aaatttctat atcagtatag catgtagtaa aacaatatgc
     5101 aagcgttttc tttgatttgt gaaagaaatt cttacttttg agaagttttc cgtactattt
     5161 tatatatcag tataaatgac ggagataaaa atgtcacgta agccaagccg tgttattagg
     5221 aagatgatgt gttctcagct atttttattc agaaattgat ctctaacatt accaataaaa
     5281 aagaaaaata tcttgacaag caagggaaaa ttttgttaca ataatagacg gtacttttta
     5341 cttttggtct ctcaagagtg tacagggacg tgctgacaaa tgttgcaaaa gtacacacag
     5401 atgatatctg tcaccaagtg tatcatcacc aaaaataaaa aaacacagga gaatgtagat
     5461 gcctacaatt aaccaattgg ttcgcaaacc gcgtaaatca aaagtagaaa aatctaaatc
     5521 accagctttg aacgttggtt acaatagtca taaaaaagtt caaacaaacg tttcttcacc
     5581 acaaaaacgt ggtgttgcaa ctcgtgttgg aacaatgaca cctaaaaaac ctaactcagc
     5641 ccttcgtaaa ttcgctcgtg tacgtttgag caaccttatc gaagttactg cctacatccc
     5701 aggtatcgga cacaacttgc aagagcacag cgtggtgctt cttcgcggtg gacgtgtaaa
     5761 agaccttcca ggggtacgtt accatatcgt ccgtggtgca cttgatactg caggtgttaa
     5821 cgatcgtaaa caaggccgtt ctaaatacgg tactaaacgt ccaaaagcat aaggaaaggg
     5881 gataaagaga aatgagtcgt aaaaatagag ctccaaaacg tgacgtattg ccagatccgc
     5941 tatacaattc acaactagtt actcgtctta tcaaccgcgt tatgcttgat ggtaaacgtg
     6001 gtactgctgc ttcaatcgtt tacggtgctt ttgagcaaat caaagaagct actggcaacg
     6061 atgcacttga agtatttgaa acagctatgg aaaacatcat gcctgtactt gaagtacgtg
     6121 cacgtcgtgt tggtggttct aactaccaag tcccagttga agttcgtcca gaacgtcgta
     6181 caacacttgg acttcgttgg ttggtaacaa tcgctcgtct tcgtggtgaa cacacaatgc
     6241 aagaccgtct tgcaaaagaa atcttggatg ctgctaacaa cactggtgca gcagttaaga
     6301 aacgtgaaga tactcaccgt atggctgaag ctaaccgtgc attcgcacac ttccgttggt
     6361 aagataggat gcgaaagcgt taagaaagtc ccagagaaaa tagggaatcg aagcaggttg
     6421 cggttgcaac caatgagatt catctttttc tccagacttt tagcttgagc tcaactaaat
     6481 catgatgcta ggaacggtaa ggatgcaagg taaaaatagg aaactgacgc agtattcgac
     6541 gaatacaagg agttttatct ttttcacgca gcatcccgtt ccagctcaca tcggctaact
     6601 aactttagcc cgggttcaaa ttagctaaat cgattagtat tagctataac tcagcttacc
     6661 atctcgtaag ttgaaaccaa caatagcatg aaaacattga gaacgggtag gtcctgccta
     6721 tccgttttta ttaaaatcgt gttataatag aatagaaatc aaaaataaat aggagaaaca
     6781 aacctcatgg cacgcgaatt ttcacttgaa aaaactcgta atatcggtat catggctcac
     6841 gtcgatgccg gtaaaacaac aactactgag cgtattcttt actacactgg taaaatccac
     6901 aaaatcggtg aaactcacga aggtgcgtca caaatggact ggatggagca agagcaagaa
     6961 cgtggtatca cgatcacatc tgctgcgacg acagctcaat ggaacaacca ccgcgtaaac
     7021 atcatcgaca caccaggaca cgtggacttc acaatcgaag tacaacgttc tcttcgtgta
     7081 ttggatggtg cggttaccgt tcttgactca caatcaggtg ttgagcctca aactgaaaca
     7141 gtttggcgtc aagcaactga gtacggagtt ccacgtatcg tatttgccaa caaaatggac
     7201 aaaatcggtg ctgacttcct ttactctgta agcacacttc acgatcgtct tcaagcaaat
     7261 gcacacccaa tccaattgcc aatcggttct gaagatgact tccgtggtat cattgacttg
     7321 atcaagatga aagctgaaat ctatactaac gaccttggta cggatatcct tgaagaagac
     7381 atcccagctg aataccttga ccaagctcaa gaataccgtg aaaaattgat tgaagcagtt
     7441 gctgaaactg acgaagaatt gatgatgaaa tacctcgaag gtgaagaaat cactaacgaa
     7501 gaattgaaag ctggtatccg taaagcgact atcaacgttg aattcttccc agtattgtgt
     7561 ggttcagcct tcaaaaacaa aggtgttcaa ttgatgcttg atgcggttat cgactacctt
     7621 ccaagcccac ttgacatccc agcaatcaaa ggtattaacc cagatacaga cgctgaagaa
     7681 attcgtccag catctgacga agagccattt gcagctcttg ccttcaagat catgactgac
     7741 ccattcgtag gtcgtttgac attcttccgt gtttactcag gtgttcttca atcaggttca
     7801 tacgtattga atacttctaa aggtaaacgt gaacgtatcg gacgtatcct tcaaatgcac
     7861 gctaacagcc gtcaagaaat cgacactgtt tactcaggtg atatcgctgc tgccgttggt
     7921 ttgaaagata ctacaactgg tgactcattg acagatgaaa aagctaaaat catccttgag
     7981 tcaatcaacg ttccagaacc agttatccaa ttgatggttg agccaaaatc taaagctgac
     8041 caagacaaga tgggtatcgc ccttcaaaaa ttggctgaag aagatccaac attccgcgtt
     8101 gaaacaaacg ttgaaactgg tgaaacagtt atctcaggta tgggtgaact tcaccttgac
     8161 gtccttgttg atcgtatgcg tcgtgagttc aaagttgaag cgaacgtagg tgctcctcaa
     8221 gtatcttacc gtgaaacatt ccgcgcttct actcaagcac gtggattctt caaacgtcag
     8281 tctggtggta aaggtcaatt cggtgatgta tggattgaat ttactccaaa cgaagaaggt
     8341 aaaggattcg aattcgaaaa cgcaatcgtc ggtggtgtgg ttcctcgtga atttatccca
     8401 gcggttgaaa aaggtttggt agaatctatg gctaacggtg ttcttgcagg ttacccaatg
     8461 gttgacgtta aagctaagct ttatgatggt tcatatcacg atgtcgactc atctgaaact
     8521 gccttcaaga ttgcggcttc actttccctt aaagaagctg ctaaatcagc acaaccagct
     8581 atccttgaac caatgatgct tgtaacaatc actgttccag aagaaaacct tggtgatgtt
     8641 atgggtcacg taactgctcg tcgtggacgt gtagatggta tggaagcaca cggtaacagc
     8701 caaatcgttc gtgcttacgt tccacttgct gaaatgttcg gttacgcaac agttcttcgt
     8761 tctgcatctc aaggacgtgg tacattcatg atggtatttg accactacga agatgtacct
     8821 aagtcagtac aagaagaaat tattaagaaa aataaaggtg aagactaatc cgtcctcact
     8881 ctagaaggaa gtcacttagt ggcttccttt tgtctttaga aaatacctct aaatatggta
     8941 aaatagtaga agaataatgt gaggaaaatg aatgtcaaat agttttgaaa ttttgatgaa
     9001 tcaattgggg atgcctgctg aaatgagaca ggctcctgct ttagcacagg ccaatattga
     9061 gcgagttgtg gttcataaaa ttagtaaggt atgggagttt catttcgtat tttctaatat
     9121 tttaccgatt gaaatctttt tagaattaaa gaaaggtttg agcgaagaat tttctaagac
     9181 aggcaataaa gctgtttttg aaattaaggc tcggtctcaa gaattttcaa atcagctctt
     9241 gcagtcctac tatagggagg ctttctctga aggtccatgt gctagtcaag gttttaagtc
     9301 cctttatcaa aatttgcaag ttcgtgctga gggtaatcag ctatttattg aaggatctga
     9361 agcgattgat aaggaacatt ttaagaagaa tcatcttcct aatttagcca aacaacttga
     9421 aaagtttggt tttccaactt ttaactgtca agtcgagaag aatgatgtcc tgacccaaga
     9481 gcaggaagag gcctttcatg ctgaaaatga gcagattgtt caagctgcca atgaggaagc
     9541 gctccgtgct atggaacaac tggagcagat ggcacctcct ccagcggaag agaaaccagc
     9601 ctttgatttt caagcgaaaa aagctgcagc taaacccaag ctggataagg cggagattac
     9661 tcctatgatc gaagtgacga cagaggaaaa tcgtctggta tttgaagggg ttgtttttga
     9721 tgtggagcaa aaagtgacta gaacaggtcg tgttttaatc aactttaaaa tgacggacta
     9781 tacttcaagt ttttctatgc aaaagtgggt taaaaacgag gaagaggccc agaagtttga
     9841 cctcatcaag aagaattctt ggctccgagt tcgagggaat gtggagatga ataacttcac
     9901 acgcgatttg actatgaacg tacaggatct gcaggaagtt gttcactatg agcggaagga
     9961 tttgatgcca gaaggtgagc gtcgggttga gtttcatgct catactaaca tgtcgactat
    10021 ggatgctttg ccagaggtcg aagagattgt tgcaacagct gctaagtggg gacacaaggc
    10081 ggttgctatc acggaccatg ggaatgtcca gtcctttcca catggctata aggcggctaa
    10141 gaaagcggga atccagctga tctatgggat ggaagccaat atcgtggagg accgtgtccc
    10201 tatcgtctat aacgaagtgg agatggactt gtcagaagca acctacgtgg tctttgacgt
    10261 ggaaacgacg ggactttcag ctatctataa tgacttgatt caggttgcgg cttctaagat
    10321 gtacaagggg aatgttattg ctgaatttga tgaatttatc aatcctgggc atcccttgtc
    10381 agcctttact acagagttaa ctggaattac agatgatcat gtcaaaaatg ccaaaccact
    10441 agaacaagtt ttgcaagaat tccaagaatt ttgcaaggat acggtcctag ttgcccacaa
    10501 tgctaccttt gacgttggct ttatgaatgc taattatgag cggcatgatc ttccaaagat
    10561 tagtcagcca gttattgata cgctggagtt tgctagaaac ctctatcctg agtataaacg
    10621 ccatggtttg gggcctttga ccaagcgttt tggtgtggcc ttggaacatc accacatggc
    10681 caactacgat gcggaagcga ctggtcgtct gcttttcatc tttatcaaag aggtagcaga
    10741 aaaacatggt gtgaccgatt tagctagact caacattgat ctaatcagtc cagattctta
    10801 caaaaaagct cggatcaagc atgcgaccat ctatgtcaag aatcaggtag gtctaaaaaa
    10861 tatctttaag ctggtttcct tgtctaatac caagtatttt gaaggagtgc cacggattcc
    10921 gagaacggtt ctagatgccc atcgagaggg cttgatttta ggttcagcct gttcagaggg
    10981 tgaagttttt gacgtggtcg tttctcaagg tgtggatgcg gcggttgagg tggccaagta
    11041 ttatgatttt atcgaggtca tgccaccggc tatctatgca cccttgattg ccaaagagca
    11101 ggtcaaggat atggaggaac tccagaccat tatcaagagt ttgatagagg ttggagaccg
    11161 ccttggcaag cctgttctgg ctacgggaaa tgttcactat atcgaaccgg aagaagagat
    11221 ttatcgtgaa attatcgtcc gtagtttggg acagggtgcg atgattaatc gaactatcgg
    11281 tcatggtgaa catgcccaac cagcaccact tccaaaggct cattttcgaa cgactaatga
    11341 gatgttggat gaatttgcct ttttgggaga ggaactggct cgtaaactgg ttattgaaaa
    11401 caccaatgcc ttggcagaaa tatttgaatc cgttgaagtc gttaagggtg acttgtatac
    11461 gcctttcatc gacaaggctg aagaaacagt tgctgagttg acctataaga aagcttttga
    11521 gatttatgga aatccgctgc cagatattgt tgatttgcgg attgaaaaag aattaacatc
    11581 catactgggg aatggatttg ctgtgattta tctggcatcg cagatgctgg tgcaacgttc
    11641 taatgaacgg ggttatttgg ttggttctcg tgggtctgtc ggatctagtt tcgttgcgac
    11701 catgattggg attacggagg tcaatcctct ctctcctcac tatgtctgtg gtcagtgtca
    11761 gtacagtgag tttatcacag atggttcgta cggttcagga tttgatatgc cccataagga
    11821 ctgtccaaac tgtggtcaca aactcagtaa aaacggacag gatattccgt ttgagacctt
    11881 ccttggtttt gatggggata aggttcctga tattgacttg aacttctcgg gagaagatca
    11941 gcctagcgcc cacttggatg tgcgtgatat ctttggtgaa gaatatgcct tccgtgcggg
    12001 aacggttggt acggtagctg ccaagactgc ctatggattt gtcaagggtt acgagcgaga
    12061 ttatggcaag ttttatcgtg atgcagaagt agaacgcctc gctcaaggag cggcgggtgt
    12121 caagcggaca acaggccaac acccgggggg aatcgttgtt attccgaact acatggatgt
    12181 ctacgatttt acgcctgtcc agtatccagc agatgatgtc acggctgaat ggcagaccac
    12241 tcactttaac ttccacgata tcgatgagaa cgtcctcaaa ctcgatgtac tgggacatga
    12301 tgatccgacc atgattcgaa aacttcagga tttgtctggt attgacccta ataaaattcc
    12361 tatggatgac gaaggcgtga tggcactctt ttctgggact gatgtactag gggtaacacc
    12421 tgaacaaatt ggaacgccta cgggtatgtt ggggattcca gagtttggaa caaatttcgt
    12481 acgtggaatg gtagacgaaa cccatccgac aacctttgcg gaattgcttc agctgtctgg
    12541 tctgtcccac ggtactgacg tttggttggg gaatgctcag gatctgatta agcaaggaat
    12601 agcggaccta tcgactgtta tcggttgtcg ggacgacatc atggtttacc tcatgcatgc
    12661 gggtctggaa cctaagatgg cctttaccat tatggaacgg gtacgtaagg gtttgtggct
    12721 aaagatttca gaagaggaga gaaatggcta tatcgaagcc atgaaggcta ataaggtgcc
    12781 agagtggtat atcgaatcct gtgggaaaat taagtacatg ttccctaagg cccatgcggc
    12841 agcctacgtt atgatggcct tgcgtgtagc ttacttcaag gttcaccatc ctatttatta
    12901 ctactgtgct tacttctcca ttcgtgctaa ggcttttgat atcaagacca tgggtgcggg
    12961 cttggaggtc atcaagcgca gaatggaaga aatctctgaa aaacggaaga acaatgaagc
    13021 ctctaatgtg gaaatcgatc tctatacaac tcttgagatt gtcaatgaga tgtgggaacg
    13081 aggtttcaag tttggtaaat tagatctcta ccgtagtcag gcgacagagt tcctcatcga
    13141 cggggatacc cttatcccac catttgtagc aatggatggt ctgggagaga acgttgccaa
    13201 gcaactggtg cgggcgcgtg aagagggaga attcctctct aaaacagaac tacgcaagcg
    13261 tggtggactc tcatcaacct tggttgaaaa gatggatgag atgggtattc ttggaaatat
    13321 gccagaggat aaccagttga gtttgtttga tgagttgttt taaaaaattg cttaataatc
    13381 tattaaaaga ggctaacgta tatccaatag atttacatta gctttctttt ttgttaaaat
    13441 agtctatgga aagagggtga gagtatgtca aagatgagta taagcatccg tctggatagt
    13501 gaggttaagg agcaggccca acaggtgttt agtaatctgg gaatggatat gacaacagct
    13561 attaatattt tccttcgtca ggcaattcaa tatcagggat taccttttga tgttagacta
    13621 gacgaaaatc ggaagttgct ccaagcgtta acggatttag accaaaatcg taatatgagc
    13681 cagtcttttg aatcagtctc agatttgatg gaggacttac gtgcttaaga ttcgttatca
    13741 taaacagttt aaaaaagatt ttaagttggc tatgaagcgt ggtttgaagg cagaattatt
    13801 agaagaagtt ttgaattttc tggttcaaga aaaagaacat cctgccagat atcgtgatca
    13861 ttcattgacg gcatccaagc attttcaagg agttcgtgaa tgccataccc agccagattg
    13921 gcttttggtt tataaagtag acaagtcgga attgatttta aatttgctga ggacaggcag
    13981 tcacagtgat ttattttaat ctattttaag ggggttctca tgaaactaag aatatttgcg
    14041 gaagataagc cggctaagaa ggtatttgaa tatcaattag aacttgctga tcgtacaatt
    14101 cttctatcga cagcactctt gtcaggtgct attgctttag caggaatctt ttctgctttg
    14161 aaagaaaaat aaaaatagaa aagagaaaac agaatggttt taccaaattt taaagaaaat
    14221 ctaggaaaat atgcgaaatt gttggttgcg aacggaatta acgtgcaacc tggtcacact
    14281 ttggctctct ctattgatgt ggagcaacgt gaattggcac atctaatcgt gaaagaagct
    14341 tatgccttgg gtgcgcatga ggtcatcgtt cagtggacag atgatgtgat taaccgtgag
    14401 aaattcctcc atgccccgat ggagcgtttg gacaatgtgc cagaatacaa gattgctgag
    14461 atgaactatc ttttggagaa caaggctagc cgtcttgggg ttcgttcatc tgatccaggt
    14521 gccttgaacg gagtggacgc tgacaagctt tcagcttctg ctaaagctat gggacttgcc
    14581 atgaagccta tgcgtatcgc aactcaatct aacaaggtta gctggactgt agcagccgct
    14641 gctggacttg agtgggctaa gaaagtcttc ccaaatgctg cgagcgacga agaagcagtt
    14701 gatttccttt gggatcaaat cttcaaaact tgtcgtgtct acgaagcaga tcctgttaag
    14761 gcttgggagg aacatgcagc cattctcaag agcaaggccg atatgcttaa taaggagcaa
    14821 ttttcagccc ttcactacac agcgccagga acagatttaa cacttggttt gccaaagaac
    14881 cacgtttggg aatcagctgg tgctgtcaat gcacagggcg aagaattctt gccaaatatg
    14941 ccgacagaag aggtcttcac agcgcctgac ttccgtcgtg cagatggtta tgtcacttct
    15001 acaaaaccgc ttagctacaa cggaaatatc attgaaggca ttaaggtgac ctttaaggat
    15061 ggacaaatcg tagatatcac tgctgagaag ggtgatcagg ttatgaaaga ccttgtcttt
    15121 aaaaatgcgg gtgcgcgtgc cttgggtgaa tgtgccttgg taccagatcc aagtccaatt
    15181 tctcagtcag gcattacctt ttttaacacc cttttcgatg aaaatgcgtc aaaccacttg
    15241 gctatcggtg cagcctatgc gactagcgtt gttgatggag cggagatgag cgaagaggag
    15301 cttgaagctg cagggcttaa ccgttcagat gttcacgtag actttatgat tggttctaac
    15361 caaatggata tcgatggtat tcgtgaggat ggaacgcggg tacctctttt ccgtaatggg
    15421 aattgggcaa attaaggaga taatatgtta ggaagtatgt tcgttggtct cctagtggga
    15481 tttttagcag gtgctatgac caatcgtgga gagcgaatgg gatgttttgg aaaaatgttt
    15541 ctcggttgga tcggagcctt tctaggtcac ttgctctttg gaacttgggg gccagtttta
    15601 tcaggaacag ctattatccc agcgatttta ggagccatga ttgttttagc tattttttgg
    15661 agacgaggaa gttaatttcc taaatctgat actcaatgaa aatcaaagag caaactagga
    15721 aactagccgc aggttgctca aagcactgct ttgaggttgc agatagaact gacgaagtca
    15781 gtaacatata tacggcaagg cgacgttgac gcggtttgaa gagatttttg aagagtatga
    15841 aacgaaaagg aggttggtca ttgtactagc ctccttttga tatgatataa tagttctatg
    15901 agattagata aatttttagt tgcctgtgcg gtgggaagtc ggactgaggt caaaaacttg
    15961 ctcaaggctg ggcgcgtgac tgtaaatggt aaaaaagaaa aatcagctaa attgcagatt
    16021 gatgaaaaaa tagatgagat tcgctttgat gggcaagtgt tggagtatga agagtttgtc
    16081 tactacatga tgaacaagcc caaaggagtt atctcagcga ctgaggatcc caagcacaga
    16141 accgttctgg acttgttgga tgacttggcg cggagtaagg aagtttttcc agtaggacgc
    16201 ttggatattg acacgcatgg tcttttgctc ttgaccaata atggtcagct ggctcatgtt
    16261 cttctttcgc ccaagcgtca tgtggacaag acttatcagg ctcaggtcaa gggaattatg
    16321 acccaagaag atgtggagac atttatcaag ggcattccgc tcaaagactt tacctgtcag
    16381 cccgctagac tggagattct ttccacagat gcagaaaaga atcagagcca aatccgtgtg
    16441 accattgcag aagggaagtt tcatcagatc aagcgtatgg tgggctactg tggcaaggaa
    16501 gtggtggact tgcaacgatt gactatggga actttagtat tagatgagaa cctggaacga
    16561 ggggaatggc gtcgcttgac caaggaagaa ttagaaattc tccgcgctaa tattatttaa
    16621 tttagtttaa tactcaatga aaatcaaaga gcaaactagg aaactagccg caggctgtac
    16681 ttgagtacgg caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tataaattcg
    16741 aaaaaggtga gggacaatgt cctcgccttt tatgtttttt agttgcttcc tttgtgaaaa
    16801 gagttataat agactgtaga ataaaaggag gaatctatga acgcgattca agaatcattt
    16861 actgataaac tatttgccaa ctatgaagca aatgtaaaat accaagcgat tgaaaatgct
    16921 gccagccaca acggaatttt tgcagctctg gaatgtcgcc aaagccatgt agacaacaca
    16981 cctgttttct cattggattt aaccaaggac aaggtcacta accagaaagc gtctggtcgt
    17041 tgctggatgt ttgcggctct caacaccttc cgccacaaac tcatctcgca atacaaattg
    17101 gagaactttg agttgtcaca agcccacact ttcttctggg ataagtatga gaaatcaaac
    17161 tggttcttgg agcaagtcat tgcgacttca gaccaagaat tgactagccg caaggttagc
    17221 ttcttactcc aaacacctca acaagatggc ggtcaatggg atatggtcgt ttccctcttt
    17281 gaaaaatacg gtgtcgtgcc taagtcagtt tatcctgagt ctgtttcatc tagcagcagt
    17341 cgtgagctaa atgcgatcct taataaattg cttcgtcaag atgctcaaat cttgcgtgat
    17401 ttgcttgttt ctggtgcaga tcaagcgact gttcaagcta agaaagaaga cctcttgcaa
    17461 gaaatcttta actttcttgc tatgtcatta ggacttccac cacgcaagtt tgactttgct
    17521 tatcgcgata aagataacaa ctataaaagt gaaaaaggaa tcacaccaca agagttttac
    17581 aagaaatatg tcaatcttcc tttagaagac tacgtttctg ttatcaatgc tccaactgct
    17641 gataaacctt acggaaaatc ttacacagtt gagatgttgg ggaatgtggt tggtagccgt
    17701 gcagttcgct acatcaacgt tccaatggag cgcttgaaag aattggcgat tgcccaaatg
    17761 caagcaggtg agactgtttg gtttggttct gatgtcggcc agctcagcaa ccgtaaggct
    17821 ggcatccttg cgacagatgt ttatgacttt gaatcaagca tggacattaa acttactcaa
    17881 gacaaggctg gacgtttgga ctatagcgaa agcttgatga cccacgccat ggtcttgaca
    17941 ggtgttgact tggacgaaaa tggtaaatca accaagtgga aggttgaaaa ctcatgggga
    18001 gacaaggtcg gtacagatgg ttactttgtt gcctcagacg cttggatgga cgaatacaca
    18061 taccaaatcg ttgttcgtaa ggaattgctg acagcagaag aacaagctgc ctatggagca
    18121 gaaccaatcg tacttgcacc atgggatcca atgggagcct tggctgaata aaagcataga
    18181 aaaaaggaat cagattttag aacctggttc ctttttagtt gcttgattac atgatgtgaa
    18241 gaacatgtgc cacaataccc actgcgaaga gggcaaggat aatagtgatt ggagatactt
    18301 ttttcttaag taagtacatg caaagtaaag taaggagtag tcctgataat ccaggaatca
    18361 acatatccaa gttttgttgg aaagtagtaa ctttttcagg agtttgagac aatccttgtc
    18421 ctacttgtgc gaatgcttct tggatacctt tagacccttc tggcaattta tcccaatgga
    18481 tataagcctt ttcatctagt tgaactttag aaacatcgaa agcaaattta atatttaccc
    18541 agcgttgaac aaggacagca agaatgaaca tcccaaggat agaagctcct ttagtgatat
    18601 cttgaaggat accaccagac atatctttag tgatttctga tccagccttg tatccaatct
    18661 cttgaacata ccacaagaat gacatacgaa tcaagttcca tgcaacaaag aagaggagtg
    18721 gccccaagat attgccagta agggcaagtg aagcaccgag agatccaagg attgggcgta
    18781 ctgtaaacca gaatactggg tcaccgatac cagcaagagg tcccatcata ccgattttaa
    18841 ccccttggat agcagcgtca tcgatttcca caccgttagc acgttcttct tcaagcgcaa
    18901 gagtaacccc catgactgga gcagctacgt atggatgagt gttgaagaac tcaaggtgac
    18961 gctcaagagc agcgatttga tcttcttttt tagtatagag ttttttgata gctggaatga
    19021 gtgtataagc ccagcccaag ttttgcatcc gttcaaagtt ccaagaccct tgtaagaagg
    19081 ttgaacgcca ccaaactttt ttacgatctg atttagttaa ttgaagtttt tcagtcatga
    19141 tgttttcagt cctttcttat cttagtagtc ttctaggata tcgccgattg ggtcgttaga
    19201 agttgcggct cctccgccac catttccacc agttttagaa aggtgaaggt agataagagc
    19261 gatagcaaca ccgatagcac cgaatccgat tagagtaata tctgacacag cagcaagaac
    19321 gaaaccaaga gcgaagaatg gccatacttc acgagttgcc atcatgttga taaccatggc
    19381 gtaaccaacg gcaacgacca taccaccacc gatagccata ccatctttga gccagtctgg
    19441 catggcacta aggatacttt gtacagtttc agttggtacc ataaggagaa gagctgcagg
    19501 aagcgcgata cgaagtcctt ggaaaagtag cgcaatgaaa tgagcacgct ccacagcgcc
    19561 gaagtcacct tttttagcgg cagcatcagc agtgtgaacc aatccaactg aaattgtacg
    19621 aacaatcata gttaagaata gaccagctac ggcaagaggg atagcaaccg cttgggcaac
    19681 accgatacca gtcttggtaa agtcaccacc aagaaccatg ataatggcag cagcgacaga
    19741 agcaagtgca gcatcaggag cgatagcagc accgatattt gaccaaccaa gggcaatcat
    19801 ttgaagcgat ccaccgagga taatccctgc ttccaagtga cctgttacaa gcccaataag
    19861 ggtacaggct acaagtggtt ggtgaaattg gaactggtcg aggatgcctt caagacctgc
    19921 aaagaaggct acaacgacta ctaaaaccat agaaataata gacatgttta aaatcctttc
    19981 ataaataatg gcttatttga cattggcttt gttaatcaag tcaaacaaat cttttttaga
    20041 atcatttggt actttacgga catcaaattc aacacccaag tcacgcattt tttcaaatgt
    20101 agcaacatct tctttgtcca tagacaaaac ggtattgacc aatgttttac ctgttgagtg
    20161 agccatagaa ccaacgttaa gagtcttgat tggcacgccg ccttcgatgg cacgaagggc
    20221 atcttgaggt gtttcaaaca agataagggc atgtgtttct ccaaaacgtg ggtcttttga
    20281 aatctcaatc agtttttgaa ttggaaccac gttagccttg acattacctg gagctgcttg
    20341 tttaatcaat tctttacgaa ggtcgtcttt agccacgtta tctgaagcaa cgatgatacg
    20401 atttgctttt gaatctggag tccaagcagt tgcaacctga ccgtgaagta gacgtgtgtc
    20461 aagacgggca agattgattt tcaatttacc gtctccgata acagttcctt ctgggatagc
    20521 agtttgggca actggagcag ctgcagcgct tgcaacttct tcaactggat ttagctcttc
    20581 tggaagagct ttgatgccat ctttggcttc tttaatgata ttagcagcga ctttttctac
    20641 acctgcagca gcgtccatga ggcgctctgt gtaggcttga atcaacatcg gtaagttaag
    20701 tcctgtgatg atggcaaact tacgctcagg attttctccc atcacgcgac tagcttggtt
    20761 aaatggagaa ccactccaaa ggtcagccaa aactagaacc tcatcttctg cgtcaaatgc
    20821 agcaacagcg ttattaaact tagcgtatag atcatcagga ccttcatttg gcataaaggt
    20881 cacaacttga accttttctt gttcaccaaa gatcatagat cctgactgat gaatacccgc
    20941 agcaaattcg ccgtggctcg caataatgat tccgatactc attattgtca ttcctccttt
    21001 tttgttttag ttttcattta agaaaacttt aagactgttt aagtataaac cgttttcata
    21061 aaaaaagcaa ctatttatca taaaaaatta aaaaattttt atttttgtat gttgatatgt
    21121 tgagtttttg aggagtaatc ttgaattctt atttaaaatt taatataaaa aagttggaag
    21181 aagcttccaa cttttaaaat aggtttctat taaattagtg ggtgaagtcg agtaccatac
    21241 gtccttggat ttggcctttt tccatttcgt cgaaaatggc aacagcatct tctactggac
    21301 gtttttgaac aactgggact accagacctt ctgcaccaaa ttggaaggct tcttctaagt
    21361 ctttacgagt tccgacaaga gaaccgatga cttggattcc atcgaggact gttttaacga
    21421 tgctgagttc catcatttca gaaggaagac caacagcgac gacgcgacca ccagcacgaa
    21481 tggagtcaac agcctggttg aaggcaactt tagacacagc agttacgaca gctgaatgag
    21541 ctcctccatc agttttttct ttaatgagtc cagctacatc ttcaacttcg aggccgttaa
    21601 tcacaatgtc agcgcctact tcttttgcaa gggtaagttt gtcattgttg atatcgactg
    21661 cgataacatg agcattgaat acttttttag cgtattgaac agcgaggtta ccaagtccac
    21721 cagcaccgta aagaacaacc cattggcctg gttcaacttt tgcttctttg atagctttat
    21781 aggttgttac tccagcacat gtgatagaag aagcttgggc tggatcaagt ccgtcaggaa
    21841 ctttgacagc atagtcagca gttacgatac attgttcagc cataccaccg tctactgagt
    21901 agccagcatt tttcactgta cggcaaaggg tttcgcgacc agttgtacag tattcgcaag
    21961 tgccacatcc ttcaaagaac caagcaacgc tgacgcggtc accgacttta aggcttttca
    22021 catctggagc aatctcttta acgataccga taccttcgtg cccaagaaca cgtcctggga
    22081 cttgaccaaa gtcaccatga gcaacgtgga ggtcggtgtg gcaaacgcca cagtattcaa
    22141 cttctacaag tgcttcccca gtttcaagtg gacggagtac tttttcttca atagcaacac
    22201 cagtgctttc tggatttaca acaacagctt tcatagctat cctccttgat attgaccgag
    22261 aacagggaca acgggactgg attgattttt tgtcaacaga gtcgagtgtg acgagctctc
    22321 ttgtatttat atgtgaagta tatcacaaaa ggaagccttt tccaaggttt tggagacaaa
    22381 aagtagaaca atcgattaac tggttgatga gactgtgaaa agagcacaaa gaccagcttg
    22441 caagctggtc atactcttcg aaaatctctt caaaccacgt cagcttcatc tacaacctca
    22501 aagcagtgct ttgagcaacc tacggctagc ttcctagttt gctctttgat tctcattgag
    22561 tatcagtata ggttataaga ataagtcttg tagggttttg gcaaccccat cttggtcgtt
    22621 ggtaagggaa atttgctcat ctgcataagg gagtagctct ggattggcat ttttcatggc
    22681 ataacccttc ccagcaaaag cgagcatttc ggtatcattg tgttcatctc caaaggcaat
    22741 caaatctttt ttgtcacgat tcattatctt gagcaagtag tccaaagcaa aggccttgtt
    22801 gacacctttt ggggtacatt caaggatatt gagcggacct ccccaggtat tgatagaaag
    22861 ttgatgctgg tagaaggcgt tcatttcttt tgccaaggca tatttgtcac tggctctggt
    22921 ctgcaagagg atacagttag ggtccttggt caccaattca ggctggaatt gatcttcagg
    22981 ctggaaagct tctacaccaa atagtttggg attggcaatt tcttcattgg gatttgtaat
    23041 gtagaatttt ttacgatatt ctccagcgat aaaatcggct tgaatgtcct ctgaacgttg
    23101 aaccatatct agcagatatt ttttgtctac agtcaaacac ttttcaaaat cccaaacttg
    23161 gtctggtaaa tgagtaaggg atccgttgaa gttaatcata ggagtgtcta agcccagttc
    23221 acggtaaaaa tcttttgaca tacggtaagg gcgacctgtc gtaataataa cctgatggcc
    23281 tttttcagca acttttttga tggttctttt ggtaaagtca gaaatttgac tgtctgagtt
    23341 gagcagggtt ccatccaggt caactgcaat aatttttttc gtcatatgga cctttctagt
    23401 gtgttttcag ataagatgtc ttctattata acagaaagca ggcagaaaag cagattccaa
    23461 acaaaaaatg aaattggatt aatttcttaa acaaatcaaa caaagacatt gaaaaagtga
    23521 atgataaatg atataattct attattgttc gtaaaaatta aaaggagatt gatgatggac
    23581 aaattattta aactaaaaga gaacggtaca gacgttcgta cagaggttct cgctggttta
    23641 acaactttct ttgcaatgag ctatattctc tttgtaaacc cacaaatact ttcacaaaca
    23701 ggaatgcctg ctcagggcgt cttcctagcg acgattattg gtgcagtagc gggtaccttg
    23761 atgatggctt tttatgctaa cttaccttat gcccaagcac caggtatggg actcaatgcc
    23821 ttctttacct ttacagttgt attcgggctt ggttattctt ggcaagaagc cctagctatg
    23881 gtcttcatct gtgggattat ttcattgatt attaccttga caaatgttcg taaaatgatc
    23941 attgaatcga ttcccaatgc tcttcgctca gctatttcag ctggtatcgg tgtcttcctt
    24001 gcctatgtag ggattaagaa tgctggactt ttgaaattca cgattgatcc aggcaactat
    24061 actgttgtag gagaaggggc tgacaaagct caagcaacga ttgcagcaaa ctcttcagca
    24121 gttccaggat tggtcagctt taataatcca gctgttttag tggctcttgc aggacttgcc
    24181 attactatct tctttgtcat caaagggatt aaagggggaa ttattctctc tatcttgaca
    24241 acaactgttc ttgctattgc agttggtttg gttgatttgt ctagtatcga ttttgctaat
    24301 aaccatgttg gtgcagcttt tgaagatttg aagacaatct ttggtgcagc tcttggttca
    24361 gaaggtttgg gagctttggt ttcagataca gctcgcttgc ctgaaactct gatggccatt
    24421 cttgccttct cattgacaga tatttttgac acaattggta ccttgatcgg tacaggtgaa
    24481 aaagttggta ttgtagcgac aaatggtgaa aatcaccaat cagccaaatt ggataaggct
    24541 ctttactctg atttgattgg aacgacagtc ggtgccattg caggtacttc aaacgtaacg
    24601 acttatgttg agtctgctgc tggtatcggt gcaggtggac gtactggttt gacagccttg
    24661 gttgtagcta tctgttttgc gatttcaagc ttctttagcc cacttctagc gatcgtacca
    24721 acagcggcta cagctccaat cttgattatc gttgggatta tgatgcttgg tagcttgaaa
    24781 aatatccatt gggatgatat gtctgaagca gttcctgcct tcttcacatc tatctttatg
    24841 ggattcagct actctatcac tcaagggatt gcagttggtt tcttgactta cactttgact
    24901 aagcttgtca aaggtcaagt taaagatgtt catgccatga tttggatttt ggatgccttg
    24961 tttatcctta actacatcag catggcctta taatagaatg acccaggggg atttcccccc
    25021 ttttttaata caaaggagat aggtgatgaa agagaaaaat atgtggaaag aattgttgaa
    25081 tcgtgcaggc tggattttgg tctttttact tgccgtcctt ttatatcagg ttcccctagt
    25141 ggttacctct attttgactt taaaagaagt agccctgcta cagtcagggc tgatagttac
    25201 tggcctttca attgtggttc tggctctatt tattatggga gctcgtaaaa ccaagttagc
    25261 tagttttaat ttttcttttt ttagagctaa agatttggca cgtttgggct tgagttattt
    25321 agttattgtc gggtcaaata tacttggttc tatcttgttg caactgtcaa atgagacgac
    25381 aacagctaac cagtctcaga ttaacgatat ggttcagaat agttctctga tttctagctt
    25441 cttcttgcta gccttgcttg ctccaatttg tgaggaaatc ttgtgccgtg ggattgttcc
    25501 taaaaagatt ttccgaggca aggagaactt gggatttgta gtcggtacga ttgtgtttgc
    25561 tttattgcat caaccaagta atttaccttc tttattgatt tatggaggta tgtcgacagt
    25621 tctatcttgg acggcctaca agacccaacg tttggaaatg tcgatcttgc ttcacatgat
    25681 tgttaatggg attgctttct gtttgttggc tcttgtggtg attatgagtc ggacattagg
    25741 aatttctgtg taaaagtttt tatgtaggaa ccgacctctt tctactaggg aaagatgaat
    25801 gcaatcgtgt ccatcttttt ctttttatgg taaaatagaa aaataatatg atgaaaatcc
    25861 ttgagggagt gaccgatatg tcaagtaaag ccaatcatgc aaagacagtt atttgcggaa
    25921 ttatcaatgt aaccccagac tccttttcgg acggtggtca attttttgct cttgagcagg
    25981 cactccagca ggctcgtaaa ttgatagcag aaggagccag tatgctcgat atcggcggag
    26041 aatcgactcg gccgggcagt agctatgttg agatagaaga ggaaatccag cgtgttgttc
    26101 cagtgatcaa agcgattcgc aaggaaagtg atgtccttat ttctatcgat acttggaaaa
    26161 gtcaggtggc agaggctgct ttggctgctg gtgccaatct agttaatgat atcactggtc
    26221 tcatgggcga tgaaaaaatg gcccatgtgg ttgctaaagc tggagcgaaa gtagtcatca
    26281 tgtttaatcc agtcatggct cgacctcaac atcctagttc gcttatcttc cctcattttg
    26341 gctttggtca aacctttaca gaaaaagagt tagctgactt tgaaacattg ccaatcgaag
    26401 acttgatggt ggctttcttt gaacgagcac tagcgagagc ggcagaagct ggtattacac
    26461 cagaaaatat cctgttggat ccaggaattg gctttggtct gaccaagaaa gaaaatctcc
    26521 tccttttacg ggacctggat aaactacatc agaagggcta tccaatcttt ctcggagtgt
    26581 cgcgcaagcg atttgtcatc aatatccttg aagaaagtgg gtttgaagtc aatcctgaga
    26641 cagagcttgg tttccgaaat cgggacacgg cttcggctca tgtaactagt atcgctgcga
    26701 gacagggtgt agaagtggtg cgcgtgcatg acgtagctag tcacaggatg gcagttgaaa
    26761 ttgcctctgc cattcgtctg gctgatgaag cggaaaattt agatttaaaa caatataaat
    26821 aagatgaaag aaattgaaaa caatcagtgg attgctaact accggacgga tcaaccgcat
    26881 tttggattgg aacgaatggt ggaactgtta gctttgcgtg gcaatcccca tctcaaactc
    26941 aaggtcctcc atatcggagg gactaacggc aagggctcga ctattgcttt tttgaaaaag
    27001 atgctagaaa agctagggct gagagttggc gtgtttagct cgccctatct cattcattac
    27061 acagaccaga ttagcatcaa tggggaatcg atctcagaag cgaggctaga agctctcatg
    27121 gcagactatc agtctttgct ggagggagaa gcggtcgcca atttacaggg cacaaccgag
    27181 tttgagatta tcacagccct ggcctatgac tactttgcct cagagcaagt agatgtggcc
    27241 atcatggaag ttggcatggg tggacttttg gatagtacca atgtctgtca gcccattttg
    27301 acaggaatta caactattgg cttggatcat gtggccctac ttggtgacac cttggaggcc
    27361 atagcagagc agaaggcagg tattatcaaa caagggatac ccttggtaac agggcgtatt
    27421 gctccagaag ccttgactgt gattgaccgc attgcggaag ggaaagatgc gccgagactt
    27481 gcctacggga cagattatca ggttcgtcat caagaaagtg tggtgacagg ggaagtcttt
    27541 gactatacaa gtgctgtcag acaaggtcgc ttccagacta gcctgcttgg tttgtaccaa
    27601 atagagaatg ctgggatggc catagcttta cttgatactt tttgtcaaga agatggtcga
    27661 gagctagcaa gcaatgactt tcttggtcaa gccttggaag aaacaagttg gccagggcgt
    27721 ttggaaatcg tgtcaagaga tcccttgatg attttggatg gagcccataa tccccatgct
    27781 atcaaggcct tgttggtaac cttgcaagaa cgttttgcgg attatcataa ggaaatcctc
    27841 ttcacttgta tcaaaaccaa agccttggag gatatgttgg acttgctggg agccatgcca
    27901 gataccgagc ttactctaac acattttgcg gatagtcggg cgacggatga aagcgtgctg
    27961 aaagaggcag ctaagtctag aaatctcagc taccaagatt ggcatgattt tctagagcag
    28021 aatttgacag ataaaaaaga agagaaacaa acagttagga ttgtcacagg ttccttgtat
    28081 ttcttgagcc aagtgagggc ctatctgatg gagaggaaga acgagaatgg atacacaaaa
    28141 gattgaagca gctgtaaaaa tgattatcga ggctgtagga gagaacgcta atcgcgaggg
    28201 cttgcaggaa acacctgctc gtgtagcccg tatgtatcaa gagatttttt caggtcttgg
    28261 tcaaacagca gaggaacatt tgtcaaaatc ctttgaaatt attgacgata atatggtggt
    28321 agaaaaggat atctttttcc ataccatgtg tgaacaccac ttcttgccat tttatggtag
    28381 agcgcacatt gcctacattc cagatggtcg tgtggcaggc ttgtctaagc tagcccgtac
    28441 ggttgaagtt tattcgaaaa aaccacaaat tcaagaacgt ttgaatatcg aagtggccga
    28501 tgccttgatg gactatctag gtgctaaagg agcctttgtt gtcattgagg cggaacatat
    28561 gtgtatgagt atgcgtggtg ttagaaaacc aggcactgca accttgacga cagtagctcg
    28621 tggtctattt gaaacagata aggacctccg cgaccaagct tatcgtttaa tggggctata
    28681 aaaagaatcc gcttcaagcg gatttttcta gaaaggaatc attatggatc aactgcagat
    28741 taaggatttg gaaatgtttg cctatcatgg tctttttcct agtgagaaag aattggggca
    28801 gaaatttgtc gtttcagcca tcctatccta tgatatgacc aaggcagcta cagacttgga
    28861 tttaacagcc tctgtccatt acggagaatt atgtcagcag tggacgactt ggtttcagga
    28921 aacgagtgaa gatttgattg aaacggtagc ctataaactg gtggaacgta cctttgagtt
    28981 ttatcctctt gtccaagaaa tgaagttgga actgaaaaaa ccttgggcac cggtgcattt
    29041 gtcactagat acttgctcgg taaccattca tcgccgcaag caacgagcct ttatcgccct
    29101 aggaagcaat atgggagata aacaagcaaa cttgaagcaa gccattgaca aactgcgagc
    29161 tcgtggcatc tatattctca aagagtccag tgtcttagcg acggagcctt ggggtggagt
    29221 ggagcaggat agctttgcca atcaagtggt tgaggtggaa acctggctac cagcacaaga
    29281 cttgttagaa accttgttag ccattgagtc agagctggga cgggtgagag aagtgcattg
    29341 gggacctcgt ttgattgatt tggacttgct ctttgtggag gaccagatcc tttatacaga
    29401 cgacctcata ttgcctcatc cttacatagc ggaacgcctt tttgtccttg agtccttaca
    29461 ggaaattgcg cctcatttta tccatccgac attaaaacaa ccgatccgca acttgtatga
    29521 tgctttgaaa aaatagaaaa actctagttt tcagttactt gcaactgaag gctagagttt
    29581 ttaaaatagg tcattttctt ctgggaggag gatagtttct ctaccgtaca tgtctaaaac
    29641 cagtactctt gggggataac gagggtcgaa aggatggtta aagtcaaaat caatggctgt
    29701 agggaggtgt tgacttgaaa agtggaaggt aatctttcct tggttattaa gcaattgaaa
    29761 ctcgagttct tcttccaatt caaagacatt ttttaagaaa tgatcgatga tataccaaaa
    29821 agagtcaatg atgtcatcgg gcaagctggt aacaatgcca aaactagcag accgcatgtg
    29881 ggtattggta aaagccatat ctctgtcccc tttcttttcc cttatcatac agcaaatagg
    29941 attaaaaatc aagaaaaggt gattttttga aaaggatttt aactacaggg aaaaataagg
    30001 aaaaaaattc ccaaaaatct accgaagtta ataggtaaat tcccaaatta acttgattat
    30061 ataactttca gtta
//
LOCUS       DAVBCB010000023        29758 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVBCB010000023 DAVBCB010000000
VERSION     DAVBCB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 29758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29758
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.023"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            132..494
                     /locus_tag="VQU28_000991"
     CDS             132..494
                     /locus_tag="VQU28_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476361.1"
                     /translation="MRLASESRQILDKLKDDVYNKLGYEVSYSSIVSQAVREYVPKKE
                     RIDWIKLKETAIPFSSLKQSNNWEYQTSLMLEKDVLILLSELQNFFLDVFQAKRIHRA
                     FCIRLCLRAQFLLSNNDS"
     gene            902..1201
                     /locus_tag="VQU28_000992"
     CDS             902..1201
                     /locus_tag="VQU28_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476362.1"
                     /translation="MNRNVLNFLRTESAERVSLYIDKANRLEGDVTLLAPSSQDLEDI
                     KNAMFSNPNLELKVARLDVMKKIAYASTRNHYLTGATIFGDISKGTYNCDPKSYV"
     gene            complement(1296..1733)
                     /locus_tag="VQU28_000993"
     CDS             complement(1296..1733)
                     /locus_tag="VQU28_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476363.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFEWLLLSGLIFFLA
                     QGLLLFLLVVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISNTYTILSISSFLV"
     gene            complement(1864..2498)
                     /locus_tag="VQU28_000994"
                     /pseudo
     CDS             complement(1864..2498)
                     /locus_tag="VQU28_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819460.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
     gene            complement(2709..3359)
                     /locus_tag="VQU28_000995"
     CDS             complement(2709..3359)
                     /locus_tag="VQU28_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HET2476364.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRMHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            3471..4313
                     /locus_tag="VQU28_000996"
     CDS             3471..4313
                     /locus_tag="VQU28_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HET2476365.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            4389..4826
                     /locus_tag="VQU28_000997"
     CDS             4389..4826
                     /locus_tag="VQU28_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HET2476366.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKKSKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTDIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            4894..5196
                     /locus_tag="VQU28_000998"
     CDS             4894..5196
                     /locus_tag="VQU28_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476367.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(5287..6360)
                     /locus_tag="VQU28_000999"
     CDS             complement(5287..6360)
                     /locus_tag="VQU28_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HET2476368.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDKLAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHKIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            6516..7193
                     /locus_tag="VQU28_001000"
     CDS             6516..7193
                     /locus_tag="VQU28_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476369.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            7186..8160
                     /locus_tag="VQU28_001001"
     CDS             7186..8160
                     /locus_tag="VQU28_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2476370.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVEMQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLLLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQEICIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(8209..10152)
                     /gene="thrS"
                     /locus_tag="VQU28_001002"
     CDS             complement(8209..10152)
                     /gene="thrS"
                     /locus_tag="VQU28_001002"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HET2476371.1"
                     /translation="MINITFPDGAIREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHGDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(10210..10386)
                     /locus_tag="VQU28_001003"
                     /pseudo
     CDS             complement(10210..10386)
                     /locus_tag="VQU28_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
     gene            complement(10393..10533)
                     /locus_tag="VQU28_001004"
     CDS             complement(10393..10533)
                     /locus_tag="VQU28_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HET2476372.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(10964..11164)
                     /locus_tag="VQU28_001005"
     CDS             complement(10964..11164)
                     /locus_tag="VQU28_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476373.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKGLYINNLL
                     FFTPMVILIVSLLYSYRFRFSL"
     gene            11403..11948
                     /locus_tag="VQU28_001006"
     CDS             11403..11948
                     /locus_tag="VQU28_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HET2476374.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGSGS
                     LYIDETKTTRLLDTPDFPEEFKSLAKAQADELDLLRTKNNLNWAFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKRNKPD"
     gene            12174..12443
                     /gene="rpsO"
                     /locus_tag="VQU28_001007"
     CDS             12174..12443
                     /gene="rpsO"
                     /locus_tag="VQU28_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HET2476375.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            13259..13873
                     /locus_tag="VQU28_001008"
     CDS             13259..13873
                     /locus_tag="VQU28_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HET2476376.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(14165..14914)
                     /locus_tag="VQU28_001009"
     CDS             complement(14165..14914)
                     /locus_tag="VQU28_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HET2476377.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            15047..17383
                     /locus_tag="VQU28_001010"
     CDS             15047..17383
                     /locus_tag="VQU28_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HET2476378.1"
                     /translation="MDKNKIMGLTQREIKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAVASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            17485..18162
                     /locus_tag="VQU28_001011"
     CDS             17485..18162
                     /locus_tag="VQU28_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HET2476379.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            18149..18946
                     /locus_tag="VQU28_001012"
     CDS             18149..18946
                     /locus_tag="VQU28_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HET2476380.1"
                     /translation="MLASEVIHAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            18963..20066
                     /locus_tag="VQU28_001013"
     CDS             18963..20066
                     /locus_tag="VQU28_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HET2476381.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            20073..20264
                     /locus_tag="VQU28_001014"
     CDS             20073..20264
                     /locus_tag="VQU28_001014"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476382.1"
                     /translation="MKIKEQTRKLAAGYSKHCFEVVDRTDEVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFELDFRRV"
     gene            20367..21386
                     /gene="galE"
                     /locus_tag="VQU28_001015"
     CDS             20367..21386
                     /gene="galE"
                     /locus_tag="VQU28_001015"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HET2476383.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAIEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            21388..22341
                     /locus_tag="VQU28_001016"
     CDS             21388..22341
                     /locus_tag="VQU28_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2476384.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRKLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDDRLKKIEFI"
     gene            22493..23158
                     /locus_tag="VQU28_001017"
     CDS             22493..23158
                     /locus_tag="VQU28_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476385.1"
                     /translation="MLSKEDYTEEIGLIKKQNYVEAELYPIVADIIKPTLKDSLSKRY
                     VFGRQRRGLGQIYYGLSNFPDIVILDKTYENKSRKSIKIEEWKKLRGCVEVKNLNYSL
                     ITEEKIKSTISNSFEHITGEMEQLTGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKE
                     EIDNTIVEVVNKRIETEEAGNSFDWWKNIKDLSFNYTDICLSKDCRQEWNEFVKRVKE
                     IEW"
     gene            23197..23298
                     /locus_tag="VQU28_001018"
                     /pseudo
     CDS             23197..23298
                     /locus_tag="VQU28_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(23349..23555)
                     /locus_tag="VQU28_001019"
     CDS             complement(23349..23555)
                     /locus_tag="VQU28_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HET2476386.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            23607..24083
                     /locus_tag="VQU28_001020"
     CDS             23607..24083
                     /locus_tag="VQU28_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HET2476387.1"
                     /translation="MAKEPWQEDIYDQEESRAERRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            24092..24763
                     /locus_tag="VQU28_001021"
     CDS             24092..24763
                     /locus_tag="VQU28_001021"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HET2476388.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDFETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            24894..25232
                     /locus_tag="VQU28_001022"
     CDS             24894..25232
                     /locus_tag="VQU28_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HET2476389.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLADGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            25235..25864
                     /locus_tag="VQU28_001023"
     CDS             25235..25864
                     /locus_tag="VQU28_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HET2476390.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITKHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            26104..27255
                     /locus_tag="VQU28_001024"
     CDS             26104..27255
                     /locus_tag="VQU28_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2476391.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            27568..28317
                     /gene="truA"
                     /locus_tag="VQU28_001025"
     CDS             27568..28317
                     /gene="truA"
                     /locus_tag="VQU28_001025"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HET2476392.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQAI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFTVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            28307..29080
                     /locus_tag="VQU28_001026"
     CDS             28307..29080
                     /locus_tag="VQU28_001026"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HET2476393.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            29058..29519
                     /locus_tag="VQU28_001027"
     CDS             29058..29519
                     /locus_tag="VQU28_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2476394.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         8719 a         5366 c         6692 g         8981 t
ORIGIN      
        1 taaattttta gattgtggaa tttttttcaa cttaattctt gatttttttt ctaaattgtt
       61 atatacttaa tttacgggat aacagaatcc cgtcagaaag gggtgataat atgggaaaag
      121 agaatgttag aatgcgttta gcgtctgaat ccagacagat attagataag cttaaagatg
      181 atgtttacaa taaattaggc tatgaagtaa gctattccag tattgttagt caagctgtta
      241 gagaatacgt tcctaaaaaa gaacgtatag attggataaa attaaaagag acagctatac
      301 ctttttcatc gttaaaacag agcaataatt gggagtacca gacatcactt atgttagaga
      361 aagatgtatt aatcttactg tctgaactac aaaatttttt cttagatgtc ttccaagcga
      421 aacgtataca tagggcattt tgtatacgtc tatgtttgag agcgcaattt ttattaagta
      481 ataatgattc ttaatactat caatattaat gcggagtgta atgctccgca aaaataaaac
      541 ctattacggg ataaaaacac tactttattt aggatggaaa taagaacgta ttttttacta
      601 atattgttta gaaaggagtt tctttatgac accagaagaa caaactgctt tggaatactt
      661 ttatgaggaa ctcgagagtt acggtgaaga tgagcaagga gaggtctttg atttgtatga
      721 taattaaatc atctccatag tttctgagaa taattgagaa actatactct aaatcatagg
      781 tttatgtaaa gctagatata cacaaaagga ggtacgaatg aagatgaaag ctagagcttc
      841 accgttgaat tgattttgtt tctagaaaaa atcaataatt agaaaaatac gaggtaaaaa
      901 aatgaataga aatgtactaa attttctgcg aacagaaagt gctgaaagag ttagtcttta
      961 tatagacaag gctaatagat tggagggcga tgtaactcta ctcgcaccta gttctcaaga
     1021 tttggaagat attaaaaatg ctatgttttc caatcctaat ctcgagctaa aagtagcgag
     1081 actggatgtg atgaagaaaa tagcatatgc ttctacaaga aatcattatt taacaggggc
     1141 tacgattttt ggggatattt cgaaaggaac atacaattgt gatcctaaaa gttatgtcta
     1201 atataaggga ggttaatccc tccccttgtt tttgaaaatc gaaaggaaaa gtataaaaat
     1261 gaaagttaat gaatatttct ggagaacgag ctgtcttata ctaaaaaaga agaaatcgat
     1321 aaaatagtat acgtgttaga gatgtgtttg tcgtaaaagt ctggatagtt ttctcttagg
     1381 tagtcagctg tcatataata aaaagtagaa tggcaagcag tgatgattgg cataagagat
     1441 gaaaaacgct gagaactaat gaaggctaag agaacaaaaa atagaaaatc aatggtgaga
     1501 acccctaaat agtagaagcg aatcttttta ttgatttgag gataaatctc agcgaattga
     1561 tgtttgagtc ggacgaccaa gagaaatagc aggagtcctt gagcaaggaa gaaaattaaa
     1621 ccagagagca agagccactc aaaagatgtc tcgagtctag tgataaaaaa tgcaaatagt
     1681 agcaaatcaa taactgcaat tgctataaaa tggattctat agagtttttt catgacaaga
     1741 cctccctctt ttatctaact tcattctact ctaaaagaat gggagttaca actaaaatga
     1801 taaaaatagc agaggggaga ttctcttaag ttggctagta ttctttattt gagtttcctt
     1861 ctattatcta acttcttcat cattccagac aaataaagct ccgattgcat tgaggatata
     1921 aaagatgtat ttaccgatat tggcgaagtt tccttgaata ccagcttttg tcagctgaac
     1981 gaaattgtaa atcaaccaaa agccccactg tccctcattg ccaatgcatt caaagcattg
     2041 gcaatgaggg acagtgcaaa ggcaatagtt gttacgtagg caaggagatt catcttgccc
     2101 ccatatccga tatagttggt cacaaaggca aagaggaagg cgatgatgga aatgatgatg
     2161 gccgccaatt ttacctgttt ttggctcatt tggttgggtc tgccttcttg cgaagctttc
     2221 catttcttta tagcaaaggt ataaatgagg aaggtgacgg gataggtaat gatggccgcc
     2281 ttatttccaa ggatataatc aatagcaccg gacaaaatgg tattaacaat accaaagtaa
     2341 ttttcccatt tgcttaattt ccccgtgaaa cgagtggaca acatggaaat cccaacgttg
     2401 gttacggaaa tcaatccaaa gggtacaaga gctgtccatg gtccccagtc tacaaattta
     2461 tcgaggcgtg agttgaggta accagatgca atcgcaatcc aacgaccaaa gcaaccccga
     2521 agaggtcaaa ctatttagat gtagcaaaaa tttttagtga ttttttcata ggttaaacta
     2581 cctttctttt tttcaaatat tctcccattt ttttttcaaa tattctacca ccaaatgaaa
     2641 gtaaaataaa atgatagaaa taaaaccctg aaaataaaac tttcagggtt ggtaggggac
     2701 ttgagaaatt agttgatttt ctcgacatag agttgttttg caatgtccat attcacttgt
     2761 aaatcctcgt cgttactgag gatagtgaag gtattgctga agccatcaaa ctgcttaact
     2821 tggagctggt caccgatgtg aagtgagtgc ttgtccagat aatggagaat gtcaaaacta
     2881 tcgtgcatcc gagtcaggcg gtaggcgcca gcttccttga tatcagctag tgggaggtta
     2941 ttgatttcaa cgaggagttc tcccttggca ggaatagttc ctccgtgggg gcaggtttta
     3001 gggaaaccta gcagtttatc tagtctttcc acgaacaggt cagagacagt gtgttccaag
     3061 acctcagctt cctcgtgaat ctggtcactt gtatagtcta aatgatgaac tagaaaaact
     3121 tcaatcaagc ggtgcttacg atagagctca gagaccagtt tgagaccgag gtcagtcagt
     3181 agatagccac attccttgtc ctttaggatg agattttcac ttttcattcg tttgatcatt
     3241 tcagttacgg cagggggaga gacttgcatg cgagccgcaa tttccttgtt ggtaatctta
     3301 tgcaggtcta tgccaatttc ataaatacat tttagatagt cttctttgtt tggggtcatt
     3361 cgtttcctct tgtctaatat ctttatctag tatatcaaaa aaagctagat ttctctagct
     3421 gtgacttttt atgttatact tattgcaaga gaaaaaacga ggagatggat atgacgaaaa
     3481 tagctcttct ttcagatatt catggaaata ccaccgcctt ggaggctgtt ttggcagatg
     3541 ctcggcagct aggagtggat gaatactggc ttttgggaga tattctcatg ccagggacag
     3601 ggcgtagaag gattttggac ttgttggatc aactaccgat tacggctaga gttttgggaa
     3661 actgggaaga cagtctttgg catggtgtcc gtaaggaatt ggatagtact cgccctagtc
     3721 aacgctatct cttgcgccag tgccagtatg ttttagagga aatttcccta gaagaaattg
     3781 aagtgctcca caatcaacct cttcaaattc atcgtcagtt tggggatttg acagtgggaa
     3841 ttagccatca tctgcctgat aagaactggg ggcgagagtt gattcatact ggcaaacaag
     3901 aggaatttga ccgcttggtg actcatcctc cctgtgatat tgctgtttat ggacacattc
     3961 accagcagtt gcttcgttac gggactggtg ggcaattgat tgtcaatccg ggttcgattg
     4021 gccaaccttt ctttttggac gctcagctac ggaaggactt gcgggcccag tatatgattt
     4081 tggagtttga tgacaagggc ctggtagata tggacttccg acgggtagat tacgatgtgg
     4141 cagctgaatt gcagctggct aaagacctga gacttcccta ttttgaggtt tactatgaaa
     4201 gtctggtcaa tgggatccac catactcatc atcaggaatt tttgagagaa ttggctcaga
     4261 aagagggttg cgatcgggag ttagacgact ggttgaaaag tggtaacgat tgacattaca
     4321 actaaaaagg gtataataat agaaaaagaa gagtagaggc agggtagcct cgtaaaaagg
     4381 agaagaggat gcaaattcca agtagattta ccattgcgac tcatatgctg ataatcattg
     4441 ccctcgaggg gaagaaaagc aaggtgacca gtgattttct ggctgctagt gtcggggtca
     4501 atcctgtcat tatcagaaag atcttgtccc agttgaagaa ggcagagctg atttcagtag
     4561 cgcgtggaac gggcggaaca gatattgtca aggaccttaa ggatattagt cttttagatg
     4621 tttatcaggc ggtcgaatgt cttggtaaga caggtcaact cttcagtttc catgacaatc
     4681 cgaatccaaa ttgccctgta ggagctcata ttcatgatgt tttggatcaa aaattggaga
     4741 gaattcagtt gactatggag gcagaacttg gtcaaaccag tctagaaaaa gtcgtggccg
     4801 atgcagagag tcagatgaag gattaagagg gataaatgcc ctcttttttc tttggataat
     4861 tatgataaaa tgtagctatt aaaggagtaa gaaatgtatc tcgttatagg aattgtatta
     4921 gcctttatag tgtcattttg gaaggataat agaagtttgt ggaatccagt attattttta
     4981 ttatccctca tttcaagtta tttttatcta tcctaccttt tttataaaaa tggatacgag
     5041 aatgttcagt tagcttttta tgtatttgcc tttgtcttac ttcctttttt gctttttcta
     5101 agtggcatct ttttaatcta taatggggtc attttgttga agcgagaggg acgttccaag
     5161 ccccattatc tctcaatgct ctttgatttt tattgactat tagattctat ttaaaaagac
     5221 ttagaatgca catgaaagta tgtattctag gtcttttgtt ttgtatacat ttaattttca
     5281 agagtattat ctatttttcc atgcctggta acgagtatca atcaataact gggtaaggcg
     5341 acccaaggtt tctctttctt ctggagtgag ggattgagct tggacaaaga gatgatgaat
     5401 gtattcaagc gcctttaagg aagccaagtc attgatagag ctaataccag cctcaagaat
     5461 agtgccaaag aagaggtcga agtagagctg aagttcttcg tcagggactg tggccaccca
     5521 ttctttaaag gttgtgtcta cttgctggct atcactgttg gtcttatcca gttggacgaa
     5581 gtgcttgtcc tcaatctgcc aactaaaggt atcgtgctgg gcgatgccac ccagggcagt
     5641 actctgaacg atgattttgt gagcaggaat ttccagcatc ataccgataa tggaaccttg
     5701 tggaatgaat atcttgcttc tatccattat cctttgataa cccgcagtct gtgtcaattc
     5761 ttgatggaga ccaggtgcat caaatgtata aactgctgtg atctgatttt gcaaactttg
     5821 ctcaatttgg ctagcagcat agatagcgag atttcctccc ttggaatgcc cagctagaat
     5881 aaccttttgc ttaggatgat gggcaaaaaa gttctttaaa tagcgaaggg cgtgcttttg
     5941 agcaggaatt tccttcatat aggtcaggtg gaaatcttcc ttccagccaa tgatactgtc
     6001 atctgtccca cgaaagacaa tcagataggt atcgaggctg acacgataag tcatagccgc
     6061 aaattgcttt tgcagttcag ggtcgatgtc gttgataaaa tgggagagtt tgcaattttt
     6121 gaagcgcttg tgttgagcca atttatctaa taattgaagg cgatttttgc tagtaagcat
     6181 ggtgggctct cttggaacct gaggtgctag atctaaaaga cgttgaggaa gtgtggagac
     6241 cagattatca aaggagaggt aggtgatttc tattaaggtt agaatgtcta actcattcaa
     6301 gggaaggtcg taataagaat catatgcgac atctttcaga tagtcaaaaa tattggccat
     6361 aagagctcct ttctttttat ttatcttagc aaatttccct caaattagct agtagcatag
     6421 cctgtttgca ctggctaaaa acaggctatt tcaaattcag tttcagacca tctagcatgg
     6481 aaaaatctgt tataataatg gaaaaggaga agcgcatgca caagatttta ttaatagaag
     6541 atgatcaggt cattcgtcaa cagattggga aaatgctctc tgaatgggga tttgaagtgg
     6601 tcctggtaga agactttatg gaagttttga gtctatttgt tcagtcggaa cctcatctgg
     6661 tcctcatgga tattggtttg cccttgttta atggttatca ctggtgtcag gaaatccgca
     6721 agatttccaa ggtacctatc atgtttcttt cttcgagaga ccaggctatg gatattgtca
     6781 tggcaatcaa tatgggggcg gatgactttg tgaccaagcc ttttgaccag caggttcttt
     6841 tagctaaggt tcagggcttg ttgcgtcgtt cctatgagtt tgggcgtgat gagagtttgc
     6901 tggaatatgc tggtgttatc ctcaatacca aatccatgga tttacattat caagggcaag
     6961 tcttgaattt gaccaagaat gaattccaga ttttacgcgt gttatttgag catgcaggca
     7021 acatcgtagc acgtgacgac ctgatgcggg aactttggaa cagtgacttt ttcattgatg
     7081 ataataccct ctctgtcaat gtggctcgtt tgcgtaaaaa gttggaggag cagggattgg
     7141 taggatttat cgagaccaag aaaggaatag ggtacggatt gaagcatgct tgattggaaa
     7201 caattttttc tagcctatct gcgctcccgt agtcgtcttt ttatctatct gctttctttg
     7261 gcatttcttg tcttactctt tcagttttta tttgccagtc taggaattta cttcctctac
     7321 tttttcttct tgtgttgctt tgtaaccatc ttatttttca cttgggacat attggtggaa
     7381 atgcaggtct atcgccagga acttctctat ggagagaggg aagccaagtc tcctttggaa
     7441 atagctttag cagaaaaatt agaagcgcgt gagatggaac tctatcagca gaggtcaaaa
     7501 gcagaaagaa aactgacgga tttgctggat tactatacct tgtgggtcca tcagataaag
     7561 acccccattg cagccagtca actcttagtt gcagaagtgg tcgaccgcca actgaagcag
     7621 cagctagaac aggaaatttt caaaatcgac tcttatacca acctagtttt acagtacctg
     7681 cgtttagaaa gtttccatga tgatttgctc ttaaagcagg ttcaaattga ggacttggtc
     7741 aaggaaataa ttcgtaaata tgctcttttc tttattcaaa aaggcttaaa tgtcaatcta
     7801 catgaccttg ataaagaaat cgtgacggat aaaaagtggc tgctagtggt tattgagcaa
     7861 atcatctcaa acagtctcaa gtacaccaag gaaggtggtc tggagattta tatggatgac
     7921 caagagattt gtatcaaaga tacgggaatc gggataaaaa acagtgatgt cctccgagta
     7981 tttgaacgtg gcttttcagg atacaatggc cgtttgaccc agcagtcctc tggacttggc
     8041 ctttatctat ctaagaaaat ttctgaagaa ctggggcacc agattcgtat cgagtctgag
     8101 gtcggaaaag gaacgacagt gcggattcag tttgctcaag tgaacttagt ccttgagtaa
     8161 aaatagatga gaaaaagcct ccagattgga ggcttttatg ctagactctt atttctcaac
     8221 gcgtgatttg ttggcgatat cagctaggat agcttgaaca aaattatcaa ctgagacagt
     8281 ttgtgtttct ttttggccgt agcgacgaac gttgactgtt tcgtcttcca tttctttgtc
     8341 tccaacaatt aattggtaag gaatcttgct ggtttgtgaa gcacggatct tgaactgcat
     8401 tttttcattg cgctcatcta cgtctgcacg gacaccgcgg tcacggagtt tcttggctac
     8461 ttcccaagcg tagtccacgt gtttttcgtt agatactggg atgagggtta cttggtgtgg
     8521 tgccagccat gttgggaagg cccccttgta gttctcaatc aagatagctg tgaagcgttc
     8581 catagttgag ataaccccac ggtggatcat gactggacgg tgatcttcgc catcagctcc
     8641 gatgtatttg aggtcgaagc gttctggcaa caagaaatca agttggatag tagaaagggt
     8701 ttcttctttt ccaagggcag ttttaatctg gatatccaat tttggtccgt agaaggctgc
     8761 ttcaccttcg gcttcaaagt agtccacgcc catttcatca agagctgcac gaagcatggt
     8821 ttgggcattt tcccacatct catcgttatc aaagtactta tgagtatctt gagggtcacg
     8881 aagagagagg cggaagcggt agtcagtcaa gttgaagtct tcataaacat cgataatcaa
     8941 ctgaagggca cgttggaatt cttcttggat ttgttctgga gtaacgaata ggtgaccgtc
     9001 gttgagtgac atttcacgta cacgttgaag gccagtgagg gcaccagatt tttcgtaacg
     9061 gtgcatcata ccgatttcag cgatacggat tggcaattca cggtaagagt gaacatggtg
     9121 tttgaaaact tggatgtggt gcggacagtt cattggacga aggacaaatt cttccccgtc
     9181 acccatgtcc atggttggga acatgtcttc ttggtaatga tcccagtgac cagaagtctt
     9241 gtaaagctca acagaagcaa gtggtggagt gtagacgtgt tggtagccag aagccaactc
     9301 tttgtttacg atgtagcgtt ccaattcacg acggatagtc gcaccatttg gcaaccagaa
     9361 tggcaaacct tgtcccactt cttgtgaaat cataaagagg tcaagctctt taccaagttt
     9421 acggtggtca cgttccttag cttcttcacg catttgaagg tagtttttca agtctttctt
     9481 gtcaaaccaa gctgtaccgt agatacgttg catcatagcg ttgtcgctgt ttccacgcca
     9541 gtacgcacca gctacatgga gaaggtggaa gatttggata cgacctgttg atggaacgtg
     9601 aggtccacgg cagaggtcta catattcacc ctgacgatag atagtcaaac cgccttcgtc
     9661 ttctgagtgt tcttcaatca attccaactt gtaagggtca tttttgaaga tttcacgtgc
     9721 ctcgtcttta gtcacttctt cacgaataga tgggaagttt tctttgacga ttttttgcat
     9781 ttcttcttcg atacgaggaa ggtcttcgtt agagatttga ccagctgtgt tgtcagtatc
     9841 gtagtagaaa ccatcttcga tggctggacc aactcccaag tgaatgtctg ggaaaagacg
     9901 acgagctgct tgggcgaaca agtgagctgc tgagtgacgc aagattggaa gggcatctcc
     9961 gtgatcaggt gtcacaattt cgatgcttcc atcttcagtg atagcgcgag tagtgtcgat
    10021 gagtttgccg ttgaatttac cagccaaggc ttttttagct agggaattgc tgatagattg
    10081 ggcaatttca aaagttgtta cgccagattc gaattcacga atagcgccat ctgggaaagt
    10141 aatgttaatc atgatgttct ccttttttat tatttcatag tttctaagca ttgatttttt
    10201 atcagcagac tacttccaag ttctaacgtt caattgcttt tctttatcag agaggaagtt
    10261 ttccatttcc gtcagaatat ctatatccaa acgttgagaa agttctaata gtcaccagat
    10321 attttctgaa agtttttgtt ccagtgtgta gggtgtttca tcatagtagc gtccttgctt
    10381 tgtcatcacc agtcattgga aatttccaat atcattagat aaagccaaga ggtcttcttc
    10441 taccgtccac ttgaaatcat gatgcttaac ttccagttcg tgataagctt gtcggattgc
    10501 ccaactgcgc tccactaatt cttgcaactc cataattagt atcctaataa aaaaatgcga
    10561 catcctcgaa aggacgtcgc aacgtggttc caccttcatt tatgtacctc aaaagagagg
    10621 gacacctctg atcggctcta acgtggccac cgttttatgt tttcataaaa aactcaagag
    10681 tagtatcagt ttagacttcc tatgcgtttc cagcaaccac gcactctcta gtagaaaggg
    10741 actaagtgac ttgtctctat ggattattat agcatgattg tgagattttt caaggatttt
    10801 gtgaaagttt tttgtttttc attttttgta gaatatgctt gatgctaata ccagaatcca
    10861 tgcccaccag agcaactgtt tcatagatta gcaagtaaat tagtataaac acaccaaagg
    10921 ttatgatagt aaagtaaaag agcagtccgt taccaactaa ccatcaaagt gagaaacgga
    10981 aacggtagct atagagtaac gatacaatca agattaccat aggtgtaaag aagagaagat
    11041 tgtttatata gagtcccttg agtgtaaggg ggtcagaaac aactagcaag aacaactcat
    11101 aaagtggcca ataaaagaat aatatgacca gatagtaggg gagatgggaa tagaatctac
    11161 gcatgataat cacctcgttt taaaaaaaca gacaacttag atctcttgct atgattatta
    11221 tagtatgttt aaaacaagaa agtaaatttg ttgacaaaga agatttttct tttcactaga
    11281 atcatggtta gttttcttgg tagaaatatt ttgataataa ttaagcaaga ctattgacat
    11341 tgattattaa gagtgtcaca attgttgtaa ctaaaataat tacaacaaga ggggtataaa
    11401 aaatgaaact agcagttatt gcagcaaatg gacaagcggg gaaggcaatt gttgaagaag
    11461 cagtcaaacg aggacatgaa gtaacggcta ttgttcgttc tgaaaacaaa agtcaagctg
    11521 agtctattat taaaaaagat ttatttgagt tgacaaaaga tgatcttact ggatttgatg
    11581 ctgttatttc agcgtttggt gcttatacac cagatacgct accgctccac agcaaatcaa
    11641 tcgaactatt taatcagctt cttgctggaa cacagacacg ttttctagtt gtagggggat
    11701 caggaagcct ttatatagat gaaacaaaaa cgacacgact tttagatacg cctgattttc
    11761 ctgaagaatt taagtcacta gcaaaagctc aagcggatga attggatctt cttcgtacaa
    11821 aaaataatct caactgggct tttgtcagtc ctgctgtaga ttttattccc gatggtgaaa
    11881 aaacaggaaa ctacattcta gcaggagaaa tttttactac taatgaaaaa aggaataagc
    11941 cagattagtt atgcagatta tgctattgga ttggtagatg aacttgaaaa aggtcatcat
    12001 ataaaagaac gtatctcttt acttgaaaaa taataactat gagagtatta atgtttgaca
    12061 gaaaatttaa atcttggtag aatagggatt gtcgtaaaga caaataactt cttcttggtt
    12121 acaggcatgc caacctgtca ctcggatgaa gccaaataaa aaggagaaac atcatggcaa
    12181 tctcaaaaga gaaaaaaaat gaaatcatcg cacaatatgc acgtcacgaa ggtgatacag
    12241 gttcagtaga ggttcaagtt gctgtcctta cttgggaaat caaccacctt aatgaacaca
    12301 tcaaacaaca caaaaaagac cacgctactt accgtggatt gatgaaaaaa atcggtcgcc
    12361 gtcgtaactt gcttgcatac ttgcgtaaaa acgacgttaa ccgttaccgt gagttgatca
    12421 actctctagg acttcgtcgc taattcaaga tacaaaggct gtcaaaagca caaagtaaaa
    12481 ataggaaaat ttacgaagaa acttcagttt ctaggagatt ttatcttttt tgccaagtgc
    12541 ttaggccgtg ttcaattgag catatcttga taatgaagct actctaaatt gggtagcttt
    12601 tttgtgttgt caccttacct cgatatactc aagtataatc tttggttacg gttcctagca
    12661 ctgtaaggta aaataaacca gcttttctcc cttcggggag attttttttg tttcactgaa
    12721 agtttcgtac gatcttcgcc tcgtcgccta gtctataaac gatttatcta gcaagaatca
    12781 tgatgctaag ggcgtcaaaa atccgtatga aaatagggaa atgacacagt gttcgatgag
    12841 cacaaggagt ttcatctttt tcactaggat tttagcccgg actcaaatca gctctctgac
    12901 ttcagagggc ttttttattg aggttttatc ggtaacaatt ttggagacta caaaaacctc
    12961 aaacggtatc agctaattac accataaagg tgcgcagtta ctcggctttt caagccgagt
    13021 agttgtatgc aagcccccta gaagagccca ctctttacaa agtaaagtat agtatgttat
    13081 actttacatg gaagtagtca ctgaattcca gttagaaatt actttgtaac tacgttttga
    13141 ggaggagtaa aatgctttcc tacgttcgat attacccact agcgatagct aaattaatgt
    13201 gtctgtgctc tcctaaaatc tgctgattta ttactgacta atacaggagt ttttttttat
    13261 gggacagaca atcatatctg ctattggtgt ttatatttcc accagtatcg attatttaat
    13321 tattttaatt attttatttg cacagctatc acagaataaa cagaaatggc atatttatgc
    13381 ggggcaatat ctaggcacag gcttacttgt aggggcgagt ttagttgctg cttatgtcgt
    13441 taatttcgtg cctgaagaat ggatggttgg attgcttggt ttaatcccta tctatttagg
    13501 gattcgcttt gcaattgttg gagaagatgc ggaagaagaa gaggaagaaa ttattgaaag
    13561 attagaacaa agcaaggcaa atcaactgtt ttggacagtt acattgctga caattgcgtc
    13621 tggcggagat aatttaggta tctatatacc ttattttgct tcgttagatt ggtcacagac
    13681 cctcgtggcc ttgcttgtgt ttgtaatcgg cataattatc ttttgcgaga ttagtcgggt
    13741 gttatcttct attccgttaa tattcgagac aattgaaaaa tacgagcgaa tcattgtgcc
    13801 cttagtattc attctacttg gactatacat catgtatgaa aatggcacga tagagacttt
    13861 tctgatcgtg tagatttttt tgtttcacta ggattttagt ccgagctcaa atcagctctc
    13921 tgattttcag agggcttttt cattgagtat aaatggacaa tcttaaagtt actctattgt
    13981 agagtggctt ttgatttgat taggagattc ttataaatat agtaaaatga aataagaata
    14041 ggacaaatcg atcaggacag tcaaatcgat ttctaaaaat gttttagaag tagaggtgta
    14101 ctattctagt ttcaatttga tatataaatg aaaaccttag ctgtttggcc aaggttcatt
    14161 tcttctatgc aagttcttct cttttcttat ctgggttcca gatgaagctt gcgataaagc
    14221 taaagatgat ggttaggata gcgagaataa tagcaaggat gagggcaggg atgcggataa
    14281 accaccagag tggatttggt tttttatcat tgtgccattg tttcgtttct tcgtccagac
    14341 gttggaattc tgtttgaata cgattggcga ctgtttcaat gccttcatca ttcattttct
    14401 tgatatctga gatatcgatt ggatttccaa agttcatatc gacacgttca cggctaatca
    14461 agcccttcaa agtcatggga ccggtgtagg taaccggcat gatacggacc ttggccattt
    14521 tggcaatcag tgctgcgccc cccttgacat cgtttgagtg gcggctacca cttggaaaca
    14581 tgatgagaga gcggtcactt tttttgagaa cgttgatagg atatttgatg gctgaggcgc
    14641 tgggattttc acggtcgatg ggaaaggcgc cacacatacg aatccaccaa ccaaagatac
    14701 ggttggtaaa gagttctttt tttgccataa agatgaactg ttttggcttg gtcgcaaagg
    14761 ccatataaac aggatcccac caggtacggt gaggcgcaac taaaatataa ttttcatctt
    14821 gattaggaat tttatcagta ttatgatagt gagcattgcc attgatggac cataagagca
    14881 atacaactaa tccacgcaaa taagtataaa acatgcgatc tccttcgatt gttttcttgt
    14941 tattattata ccttatcaaa ggagggctgg caaacttttc ccttgactag atgcatattt
    15001 gggatgaaat tagaattctg ttaaaaaaaa tgatataata gaatttatgg ataaaaataa
    15061 gattatggga ttaacccaaa gagaaatcaa ggaaagacag gctgagggtt tggtcaatga
    15121 ctttaccgca tcagccagta ccagcacttg gcaaatcgtt aaacgaaatg tctttaccct
    15181 ttttaacgct ttgaactttg ccattgcttt ggcccttgcc tttgtgcagg cttggagcaa
    15241 tctggtcttc tttgctgtta tctgctttaa cgctttttct gggattgtga ccgagctacg
    15301 agccaaacac atggtggaca agctcaatct catgaccaag gaaaaggtca aaaccatccg
    15361 tgatggtcag gaagttgctc ttaatcctga agaattagtg ctaggagatg tcattcgttt
    15421 gtctgcagga gagcagattc ctagtgatgc cttggttttg gaaggctttg cggaagtcaa
    15481 tgaagccatg ttaacgggag aaagtgattt ggtgcaaaag gaagttgacg gcttactttt
    15541 gtcaggaagt ttcctagcca gtgggtcagt tttatctcaa gttcaccatg tcggtgcaga
    15601 caactatgct gccaaactca tgcttgaggc taagaccgtt aaacccatca actcccgtat
    15661 catgaaatcg ctggacaagt tggctggttt tactgggaag attatcattc cctttggtct
    15721 ggctctcttg ctggaagcct tgcttttaaa aggcctgcct ctcaagtcat ccgttgtaaa
    15781 ctcgtcgaca gctcttttgg gaatgttgcc taagggaatt gcccttttga ccattacttc
    15841 gctcttgact gcagtgatta agttgggctt gaaaaaggtc ttggtgcagg agatgtactc
    15901 tgttgagacc ttggcgcgcg tggatatgct ctgtctggac aagacgggta ccatcaccca
    15961 aggaaagatg caggtggagg ctgttcttcc actgacggaa acttatggtg aagaggctgt
    16021 tgccagcatc ctgaccagct atatggccca tagtgaggat aaaaatccaa cagctcaagc
    16081 cattcgccag agattccaag gtcaagtagt ctatcctatg ctctctaatc ttcccttctc
    16141 cagcgaccgc aagtggggag ccatggagtt agaaggtttg ggaatagttt tcttaggggc
    16201 acctgagatg ttgctggatt ctgaggtccc agaagccaga gaggccttgg agagaggatc
    16261 acgtgtcttg gtcttagctc tcagtcagga gaaattagac catcacaaac cacagaaacc
    16321 atctgatatt caggctctag ccttgctgga aatcttggat cctattcgag agggagcggc
    16381 agagacgctg aactatctcc gttctcagga agtggggctc aagattatct ctggtgacaa
    16441 tccagttacg gtatccagca ttgcccagaa ggctggtttt gcggactatc acagctatgt
    16501 cgattgttca aaaatcacgg atgaggaatt gattgctctg gcggaggaga cagcgatttt
    16561 cggacgtgtt tcccctcatc aaaagaaact cattatccaa acgctgaaaa aagcgggtca
    16621 tacaacagct atgactgggg atggagttaa tgatatcctg gctcttcgtg aggcggattg
    16681 ttctatcgta atggcggagg gagacccggc aacccgtcag attgccaacc tggttctctt
    16741 gaactcagac tttaatgatg ttcctgagat tctttttgaa ggtcgtcgtg tggtcaataa
    16801 cattgcccac atcgctccga ttttcttgat aaagaccatc tattccttcc tgttagcagt
    16861 catctgtatt gccagtgctt tactaggtcg gtcagagtgg attttgattt tccccttcat
    16921 tccgatccag attaccatga ttgaccagtt tgtggaaggt ttcccaccat tcgttctgac
    16981 ttttgagcga aatatcaaac ctgttgagcc aaatttcctc agaaaatcca tgcttcgtgc
    17041 cctaccaagt gccctcatgg tcgtgtttag cgttcttttt gtgaaaatct ttggaacgag
    17101 tcaaggttgg tctgagttag aaatctcaac tctcctctat tatctcttgg ggtcaattgg
    17161 tttcttatcc gtatttagag cctgcatgcc attcactcta tggcgagtcc tcttgattgt
    17221 ctggtcagta ggaggcttcc tagccacagc tctcttccca agaatccaaa aactgcttga
    17281 aatttcaacc ttgacagaac aaacgttgcc tgtttatggt gtcatgatgt tggtctttac
    17341 agtgattttc atcctgacta gtcgctatca agctagaaaa taaagaaaga ctgcaatctg
    17401 tggattgcag tttttttagg tacaagattg ccagccgaaa tgtggtataa taagaggtaa
    17461 tagagttttg gaaagtgaga gaagatgatt tcaaagagat tagaattggt agcttccttt
    17521 gtgtcacagg gggctatttt actagatgtg ggaagtgacc atgcttatct gcctatcgag
    17581 ttggttgaga gaggccaaat caaaagcgct attgcaggtg aggtggtgga aggtccctat
    17641 cagtctgcgg ttaaaaatgt tgaggctcac ggcctaaagg agaaaatcca agtccgttta
    17701 gccaatggct tggcagcttt tgaagagact gaccaagtgt ctgtcattac cattgctggc
    17761 atgggtggtc gtttgattgc taggatttta gaagaaggtt tggggaagtt agctaatgta
    17821 gagcgtttga tcctccagcc caataatcgt gaagacgact tgcgtatctg gctacaggat
    17881 catggattcc agattgtagc agaaagcatc ttagaagaag ctggaaagtt ttatgagatt
    17941 ttggtggtgg aagcaggaca aatgaagcta tcagccagtg atgttcgctt tggtcccttc
    18001 ttgtccaaag aagtcagtcc agtatttgtc caaaaatggc aaaaagaagc tgagaagcta
    18061 gagttcgctc tcggacaaat cccagaaaaa aatctggaag aacgtcaagt tctagtagat
    18121 aagattcaag ctatcaagga ggtgctccat gttagcaagt gaagtgattc atgcttatga
    18181 agccttttgc cctcaggaat tttctatgga gggagacagc cgtggtctgc aaattggcac
    18241 tttagacaag ggtatccaaa gggtcatggt tgccctcgat attcgtgaag agacagtggc
    18301 agaggccatt gaaaagggtg tggacttgat tatcgtcaag cacgcgccta tctttcgtcc
    18361 tatcaaggac ttgcttgcca gccgtccgca aaatcagatt tacatcgacc tgattaagca
    18421 tgacatcgca gtttatgtca gccataccaa tattgatatc gttgaaaatg ggctcaatga
    18481 ctggttttgt cagatgctag gaatcgagga gacgacttat ctgcaggaaa caggtccaga
    18541 acgtggaatt ggacgtattg ggaatattca gcctcagaca ttttgggaat tggcccaaca
    18601 ggtcaagcaa gtctttgacc tagatagcct tcgaatggtg cattatcaag agaatgattt
    18661 gcagaagcct atttcaagag tagcaatttg tggtggaagc gggcagtctt tctataagga
    18721 tgctttggca aagggggcag atgtctatat cactggtgat atctactacc atactgctca
    18781 ggatatgctg tctgatggct tgttagcatt ggatccaggt cactatatcg aagtgctttt
    18841 tgtggaaaaa atcgcagcac tccttactca atggaaagag aaaaaaggct gggaactaga
    18901 gattttacct agtcaagcat cgaccaatcc tttccgtcat atctagttag aaagtgaaga
    18961 caatgaaaaa agttgccatt attggagcag gaattgtggg agcaacagct gcctactacc
    19021 tctcgagaga aagtgaccta gaggtgaccg tttttgacca tgagcaaggt caagccacca
    19081 aggccgcagc aggaattatc agtccttggt tttccaaacg ccgtaataaa gcctggtaca
    19141 agatggcgcg cttgggggct gatttttatg tggatttatt agctgattta gagaaatcag
    19201 gacaaaaaat cgacttttac cagcgttcgg gagtctttct cttgaaaaag gatgaatcca
    19261 agttggaaga actttatcaa ctggccctcc agcgcagaga agaatctccc ttgatagggc
    19321 aattagccat tctggaccaa gcctcagcta atgaattatt ccctggtttg cagggatttg
    19381 accgcctgct ctatgcttct ggtggagcga gagtagatgg ccaactcttg gtaactcgtc
    19441 tgctagaagc cagtcatgtc aagctggtca aagaaaaagt gactctgaca ccgttagcat
    19501 caggctacca gattggtgaa gaggagtttg agcaggttat tttggcgacg ggagcttggt
    19561 tgggggacat gttagaaccc ttgggttatg aagtagatgt tcgtcctcaa aaaggacaac
    19621 tacgagatta tcagctggtc caagacatgg aagcttaccc tgttgtcatg ccagaagggg
    19681 agtgggattt gattcctttt gcaggtggga aattgtcctt aggcgctacc cacgaaaatg
    19741 acatgggatt tgatttgacg gtagatgaaa ccttgctcca acaaatggag gaggccacct
    19801 tgactcacta tctgattttg gctgaagcta cttcaaaatc tgagcgtgtt ggaatccgtg
    19861 cctacaccag tgatttctct cctttctttg ggcaggtgcc tgacttaact ggtgtctatg
    19921 cagccagtgg actaggttca tcaggcctca caactggtcc tattattggt taccatttag
    19981 ctcaactgat ccaagacaag gagttgacct tggaccctga aaactaccca attgaaaact
    20041 atgtcaaacg agtaaaaagc gaataatact caatgaaaat caaagaacaa actaggaaac
    20101 tagccgcggg ttactcaaag cactgctttg aggttgtgga tagaactgac gaagtcagct
    20161 caaaacactg ttttgaggtt gtagatagaa ctgacgaagt cagtaaccat acccatggca
    20221 aggcgacgtt gacgtggttt gaattggatt ttcgaagagt ataagcattt tactgaaatt
    20281 ttatcaggta gtttaggatg gcaaatgaca ttccctgtca aaaatggtaa aataagaaaa
    20341 aataatctga gaatcgagga aaaaagatgc aagaaaagat tttggtaact ggtggtgccg
    20401 gttttattgg aacccacact gttattgaat tgatccaagc aggccatcaa gttgttgtgg
    20461 tggataatct tgtcaatagc aatcgtaaga gtttagaagt tgttgaaaga atcacaggag
    20521 ttgagattcc tttctatgag gcagatattc gtgacacagc taccctcaga gatattttca
    20581 agcaggaaga accgactggt gtcattcact ttgctggttt gaaggctgtt ggcgaatcaa
    20641 cacgtatccc tcttgcctac tatgacaaca atatcgctgg aactgtcagc cttttgaaag
    20701 ccatagaaga aaacaactgt aaaaacatca tcttcagttc ttctgcgaca gtttacgggg
    20761 atccgcacac agtgcccatc ttggaagatt tcccactttc agtgaccaac ccatacggtc
    20821 gtactaagct catgctagag gaaattttga ctgatattta caaagcagac tcagaatgga
    20881 atgttgtctt gcttcgttac tttaacccaa tcggagccca tgagagtggt gatttgggag
    20941 aaaatccaaa cggtattcca aacaatctct tgccatatgt gactcaagta gccgttggaa
    21001 aattagagca agtgcaagtg tttggagacg attacgatac ggaagatgga acaggtgttc
    21061 gtgactatat ccacgttgtc gatttggcta agggtcacgt tgcagctttg aaaaaaatcc
    21121 aaaaaggttc aggactaaac gtttataacc ttggaactgg taaaggttac tcagttcttg
    21181 aaattatcca aaacatggaa aaagcggtgg gacgtcctat tccttaccgt atcgtagaac
    21241 gtcgcccagg tgatatcgct gcctgctact cagacccagc aaaagctaaa gcagaactcg
    21301 gttgggaagc agaactcgac atcacccaaa tgtgtgaaga cgcatggcgt tggcaaagca
    21361 agcatccaaa tggatttgaa gactaagatg atgatttcaa tcatcgtccc ttgtttaaac
    21421 gaagaggaag tacttcctct tttttatcag gctctggaag ctttacttcc agatttggaa
    21481 acagaaatcg agtatgtctt tgtcgatgat ggatcaagtg atgggacctt ggaactctta
    21541 aaggcctatc gggagcaaaa tccggcagtc cattatattt ctttctctcg aaattttggc
    21601 aaagaagcag ccctttatgc aggcttgcaa tatgcgacag gagatttggt ggtggtgatg
    21661 gatgcagacc tccaagatcc tcctagtatg ttgtttgaga tgaaaaatgt actagacaaa
    21721 aatgtagact tggactgcgt tgggacacgg agaactagtc gggagggaga acccttcttt
    21781 cgcagtttct gtgctgttct cttttatcgc ctcatgcaaa aaatcagccc agtggctctg
    21841 ccgtcgggtg tccgtgattt tcgtatgatg agaaggtctg tagtcgatgc cattttaagc
    21901 ttgaccgagt ccaatcgttt ttcaaagggg ctcttcgcct gggtgggttt caaaacctac
    21961 tatttggact atccaaatgt cgaaaggcag gctggcaaga ccagttggag ttttaggaaa
    22021 ctctttttct actccattga agggattatt aatttttcag attttccctt gattatagcc
    22081 tttgtagctg gtctcctatc ttgttttctt tctctgctga tgaccttttt tgttgtggtt
    22141 cggaccctca ttttgggcaa tccgacatct ggttggacct ctctgatggc tgttattctc
    22201 tttcttggag gcattcaact cttgaccatt gggattctcg gcaagtatat cagtaagatt
    22261 tatttagaga ctaaaaaaag accactttat cttatcaaag aaaaaagtga tgataggcta
    22321 aaaaagatag aatttatctg acatagtcac tttttgttat ttgtaattct tttagttcgg
    22381 agtttgggtg atttttaaaa gaattaggga tttgaaatga tttaaaaaat ctatgtagac
    22441 taaatttaaa ttgtagtgac ttaataaata aaaaatttag gagtattatg ttatgctaag
    22501 taaagaggat tatactgaag aaatcgggct aataaagaaa caaaattatg tagaggctga
    22561 attgtatcct atagttgctg atataattaa gccaacttta aaagatagtt tatcaaaaag
    22621 atatgtcttc ggaagacaaa gaaggggttt gggacaaatt tattatggtc taagtaattt
    22681 tcctgatata gttattttag ataagactta tgaaaacaaa tctaggaaaa gtataaaaat
    22741 tgaagaatgg aagaaacttc gaggatgcgt ggaagttaag aatttaaatt atagtttaat
    22801 tacagaggaa aaaatcaaat caaccatatc gaacagtttt gaacatataa ctggagagat
    22861 ggaacaacta actggagaga tgggacaact tattggagac cttttatggt ataaaaaagt
    22921 aatttataca aatggaattg aatggagatt tctaagttta gatgacaagg aagagataga
    22981 taatacaatt gttgaagtag ttaacaaaag aatagaaaca gaagaagcgg gaaattcttt
    23041 tgattggtgg aagaatatta aagatttatc gttcaattat acggatatat gcctatctaa
    23101 agattgtaga caagagtgga atgaatttgt aaaaagggta aaggaaatag aatggtagaa
    23161 gtctattctc attgagaaat ttagatttta agttacagta agatttattt agagactaaa
    23221 aaaagaccac tttatcttat caaagaaaaa agtgaccttc ctgattttac agaaaaaaat
    23281 aaagtgaaaa gactataatt ttacatggaa atgtgctaaa ctagagggag tggattatcg
    23341 ccattgattt aggcttcctg gtttccaatc agggcgcgag ttgggcaatt tttaacagcc
    23401 tctaagacat cctgactagg agaaatttct ttttccagtt ggtcagggtc atcgtaaaaa
    23461 cgcacgattc cattatcgtg gtaatcaaat aaatcagaat aagtttggca aagcccacag
    23521 gcgatacatc gttcaggtat aagtgtgatt ttcatgttca tatttatatt gtaataagaa
    23581 aagtaaaaaa aaacaaggag taaggtatgg caaaagaacc gtggcaagaa gatatctatg
    23641 atcaagaaga atcaagagca gagcgtcggc atcgaaacca cggaggggct gataggatgg
    23701 ctaatcgtat tttgacgatc ctagctagta ttttctttgt aattgtggtg gtgatggtca
    23761 tcgttctcat ctatctatca tcggggggaa gtaatcgcac agcagcctta aaagactttc
    23821 atgattctga tgcaagtgta gtacaaatct catcttcaag cagttctcag cctgagcaga
    23881 gttcagagcc agaatctact tctagtagtt cagaagaagc tgctaatcct gaaggaacga
    23941 ttaaagttct cgcaggagaa ggggaagcag ctattgccgc tcgtgcagga atctccattg
    24001 ctcagttaga ggccttgaat cctgggcaca tggctacagg atcttggttt gctaatccag
    24061 gtgatgttat aaaaataaaa taggagccga tatgaaaaca attcaaattg ctattgatgg
    24121 tcctgcttcc agcggtaaga gtacggtcgc aaagattatt gctaaggatt ttggattcac
    24181 ctaccttgat acaggagcta tgtatcgtgc agcgacctat atggctctta agaaccaatt
    24241 aggagttgaa gaagtcgaag cccttctagc cttgttggac cagcatccaa tcagctttgg
    24301 acgttcagaa actggagacc agcttgtttt tgtaggagat gtggatatta cccatcctat
    24361 ccgtgaaaat gaagtgacca atcatgtttc tgctattgca gcaattcctg aagtgcgtga
    24421 gaaactggtt tctctccaac aagaaattgc ccagcaaggc gggattgtca tggatggtcg
    24481 cgatattgga actgttgtat tgccacaagc agaattgaaa attttcctag tagcttctgt
    24541 tgatgagaga gcagagcgtc gttacaagga aaatattgcc aagggaattg aaacagactt
    24601 tgaaacccta aaaaaggaaa ttgctgcgcg tgactacaag gatagtcatc gtgagacttc
    24661 tcctctcaaa caagcagagg atgctgtcta ccttgataca actggtttga acattcaaga
    24721 agtagttgaa aaaatcaaag cagaagctga aaaaagaatg taaatgtaga aaagccgata
    24781 gattgatatc ggtttttttc taaatttgtc aaaatgctga ttttagggta taatagttgc
    24841 tgttcgagaa attttgattt tcaaagaata gtgaatgatg ataaggagaa accatgaaca
    24901 atttaccaaa ttgtccaaaa tgtaactcag agtatgtcta cgaagacggt gccctactgg
    24961 tttgcccaga gtgtgctcat gagtggaatc ctgctgaagt tgcagaagta gaagaaggtc
    25021 ttgtcgctat cgatgccaac ggaaataaat tggctgatgg tgatacagta actctcatca
    25081 aggacttgaa agtaaaaggt gcgccaaaag atttgaaaca agggacgcgc gtgaaaaata
    25141 tccgcatcgt agaaggcgac cacaatatcg actgtaagat tgatggtttt ggtgccatga
    25201 aacttaaatc agagtttgtg aggaagattt aagcatgtca aaacactata aacttgtatt
    25261 ttatagccgt atcttcttgt ttctagcggc ttttacggga gtttatcttg aaattactaa
    25321 gcatggtggt tttgggatgc ttctctatta cacggttctg tccaacctct tggtaactat
    25381 ttttaccctt tatcttctaa aggttatgag ccgtgtaggt gaaaattggc aaaggccaag
    25441 tctcttgcgc ttaaaaggtg gggtcaccat gagtatcatg attacctgtg tgatttacca
    25501 tttcctcttg gcgcccattg cgactaattt ctatacccta gaaaatttcc tttgccacta
    25561 tatcgttccc atctggtttt tagcggatac cctctttttt gacaaacagg gtcaatacaa
    25621 gatttgggat ccagcagtgt ggaccatttt accctttctg tatatgatgt ttgctctttt
    25681 taatggcttg gttctaaaac tcaatattcc aaatgccaag gataatcctt tcccttactt
    25741 ctttttgaat gtgaacaagg gttggaatgt tgtgtttaag tggtgtctga ttatctttgt
    25801 tgcctatatg gtagcaggat ttattttcta ctttatcaag caaatcaaga gaaagtcatc
    25861 ctaatactct tcgaaaatct cttcaaacca cgtcagtttc catctgcaac ctcaaaacac
    25921 tgttttgaac aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtataag
    25981 atacaaggga ttcattttcg aatttaagaa ggagtctaca aaccaatgag gcttcttctt
    26041 ttcttgctta tttgataact atcaaaaaag tagaagatta agagaaatat agaaagagat
    26101 ttcatgaaac aatttttaga acgggccagc attttggctc tctccctcgt tttgattacc
    26161 tccttttcca tttcgagtgc cctaccagcc atgtttgact attatcaggg ttattctaag
    26221 gaacaaattg agctcttggt gagcttgcct tcctttggaa tcatgatgat gttactgcta
    26281 aatggtttct tagaaaaaat atttcctgag cgcttacaga ttagtttggg cttgctgatt
    26341 ttatcattga gcggtacagc tcccttctgg taccaagcct atccctttgt ctttggaaca
    26401 cggcttctct ttggtttggg tcttgggatg atcaatgcca aggccatttc tattatcagt
    26461 gaacgctacc aaggaaaaag gcgaattcag atgttagggc tacgcgcttc tgcagaggtc
    26521 gttggagctt ctctcattac cttggccgtc ggtcagttgt tggcctttgg ttggacagct
    26581 atctttctag cctatagtgc tggatttttg gtgctgcccc tttatctgct ctttgtccct
    26641 tatggaaaat caaagaaaga agtcaagaaa agagcgaagg aagcaagtcg tttaactcga
    26701 gaaatgaaag gcttgatttt taccttagct atcgaagcgg cagttgtagt ttgtaccaat
    26761 acagctatta ccatccgtat tccaagtttg atggtggaaa gaggattggg ggatgcccag
    26821 ttatctagtt ttgttcttag tatcatgcag ttgatcggga ttgtggctgg ggtgagtttt
    26881 tctttcttga tttctatctt taaagagaaa ctgctcctct ggtctggtat tacctttggc
    26941 ttggggcaaa tcgtgattgc cttgtcttca tccttgtggg tggtagtagc aggaagtgtt
    27001 ctggctggat ttgcctatag tgtagtcttg acgacggtct ttcaacttgt ctctgaacga
    27061 attccagcta aactcctcaa tcaagcaact tcatttgctg tattaggctg tagtttcgga
    27121 gcctttacga ccccatttgt tctaggtgca attggcttac taactcacaa tgggatgttg
    27181 gtctttagta tcttaggagg ttggttgatt gtaatctcta tctttgtcat gtacctactt
    27241 cagaagagag cttaggattg attcctaagt ttttcttttg atactcaatg aaaatcaaag
    27301 agcatagttg attgagtttg gaatagtacg ctgtagctgc taaaatattt ctagaaatta
    27361 atttgacttt tctaatagag ttattcatat cttatttcaa tctactatag aaagctagcc
    27421 gcaggttgct caaaatagtg ttttgaggtt gcagatggaa actgacgtgg tttgaagaga
    27481 ttttcgaaga gtatgagatt tttataccca tacaatattt gttttaggtc cttgtttaca
    27541 gactaatttc ttgataaaat agaggttatg acgagatata aagcaactat ttcctatgat
    27601 ggttatgcct ttgctggctt tcagcgccag cctcatgcgc gtagcgttca ggaagaaatt
    27661 gaaaaaacct tgaccagatt aaataaaggg caagccatta ctgttcacgg tgctggtagg
    27721 acagatagtg gggttcatgc cctgggacag gtcattcatt ttgacctgcc ttatcagatg
    27781 gatgaggaga aactccgttt tgccttggat acccagtctc ctgaagatat tgatgtgatt
    27841 tcgattgagc ttgtggcaga tgattttcat tgccgttatg ccaagcatag caagacctat
    27901 gagtttactg tggatagagg acgtcccaaa aatccgatgc gccgtcacta tgccacccac
    27961 tttccctacc cactcgatgt ggaacgaatg cagattgcaa tcaaaaagct agagggaacc
    28021 catgatttta ccggttttac agcctctggg actagtgtag aggataaggt tcgcaccatc
    28081 acagaagcta gtttaatagt cgatgagaca ggacaatttt tgacctttac cttttcagga
    28141 aatggtttct tgtataaaca gattcgcaat atggtgggga ctcttctcaa aatcggtaac
    28201 aaccgcatgc cagtagagca gattgatctc atcttggaga agaaggacag gcaacttgca
    28261 ggtcccactg cagcaccaaa tggtttgtat ttaaaggaga ttcgttatga agaataatcg
    28321 tattttagca ctttctggaa atgatatttt tagtggtggt ggactgtcag ctgatttggc
    28381 tacctatacc ttgaacggct tgcatgggtt tgtagcagtg acttgtttga cagccttgac
    28441 agaaaaagga tttgaagtct ttccaactga tgataccatt tttcaacatg aattagatag
    28501 cttgcgtgat gtggaatttg ggggaattaa gattggtctt ctccctactg tcagtgtggc
    28561 tgagaaggcc ttggacttta tcaaacaacg cccaggagta cctgtggtgt tggatcctgt
    28621 cttggtctgc aaggaaacgc atgatgtagc tgtcagtgag ctctgccaag agttgattcg
    28681 ctttttccct tatgtcagtg tgattacgcc taatctccca gaagcagaat tattatccgg
    28741 tcaggaaatt aaaaccttgg aagacatgaa aactgcagcg cagaaattgc atgatttagg
    28801 agcgccagca gtcattatca agggaggcaa tcgtcttagt caggacaagg ctgtggatgt
    28861 cttttatgat ggacagacct ttactatcct agaaaatcca gttatccaag gccaaaatgc
    28921 tggtgcaggt tgtacctttg cctctagcat tgccagtcac ttgattaaag gtgataaact
    28981 tttgccagca gtagaaagct ctaaggcttt cgtttatcgt gctattgcac aagcagatca
    29041 gtatggagta agacaatatg aagcaaacaa aaacaactaa aatcgccctt gtatccctat
    29101 taaccgccct ttctgtggtt ctaggttatt tcttaaaaat cccaacacct acaggaattc
    29161 taactctttt agatgctggt gtcttctttg cggcctttta ctttggtagt cgtgaaggag
    29221 cggtagtcgg aggactagca agtttcttga ttgacctctt atcaggctac cctcagtgga
    29281 tgttctttag cttggtcaac catggcttgc agggattttt cgcaggattt aaaggaaaaa
    29341 gtcagtggtt aggccttatt ttagcaacta ttgccatggt aggaggctac gccttgggtt
    29401 ctactttgat gaatggctgg gcagcagccc tcccagaaat tctaccaaat ttcatgcaaa
    29461 atatggtagg gatgattgta ggatttattc ttagtcaaag tatcaagaag attaagtaat
    29521 actcttcgaa aatctcttca aaccacgtca gcttccatct gcaacctcaa aacactgttt
    29581 tgagcagcct gaggctagct ttctagtttg cactttgatt ttcattgagt ataaagaggt
    29641 tgagtaaaaa gtatttcaaa taagaaaaac gcatagtatc aggtgttgaa tgaactgccc
    29701 cccaaaaagt tagacagaaa aaatctaact ttttggggtg tttttattat gaaattaa
//
LOCUS       DAVBCB010000024        28779 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVBCB010000024 DAVBCB010000000
VERSION     DAVBCB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28779
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.024"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..405)
                     /locus_tag="VQU28_001028"
                     /pseudo
     CDS             complement(1..405)
                     /locus_tag="VQU28_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            751..1704
                     /locus_tag="VQU28_001029"
     CDS             751..1704
                     /locus_tag="VQU28_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2476395.1"
                     /translation="MDFEKIEQAYTYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLISLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            1755..2945
                     /locus_tag="VQU28_001030"
     CDS             1755..2945
                     /locus_tag="VQU28_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HET2476396.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHTQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKHIGQYLAVLNGTDAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            3094..3465
                     /gene="rnpA"
                     /locus_tag="VQU28_001031"
     CDS             3094..3465
                     /gene="rnpA"
                     /locus_tag="VQU28_001031"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HET2476397.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            3440..4264
                     /locus_tag="VQU28_001032"
     CDS             3440..4264
                     /locus_tag="VQU28_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HET2476398.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAKIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            4283..5269
                     /locus_tag="VQU28_001033"
     CDS             4283..5269
                     /locus_tag="VQU28_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HET2476399.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            5320..5943
                     /locus_tag="VQU28_001034"
     CDS             5320..5943
                     /locus_tag="VQU28_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HET2476400.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPGWQLQQTSQWMNQAQRLRPHFWVYLQRDEQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            6074..7531
                     /locus_tag="VQU28_001035"
     CDS             6074..7531
                     /locus_tag="VQU28_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2476401.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            7554..7835
                     /locus_tag="VQU28_001036"
     CDS             7554..7835
                     /locus_tag="VQU28_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2476402.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            7912..8397
                     /locus_tag="VQU28_001037"
     CDS             7912..8397
                     /locus_tag="VQU28_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2476403.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            8414..9079
                     /locus_tag="VQU28_001038"
     CDS             8414..9079
                     /locus_tag="VQU28_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HET2476404.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            9083..9946
                     /locus_tag="VQU28_001039"
     CDS             9083..9946
                     /locus_tag="VQU28_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HET2476405.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKEIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            9948..10652
                     /locus_tag="VQU28_001040"
     CDS             9948..10652
                     /locus_tag="VQU28_001040"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HET2476406.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVGPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            10831..12504
                     /locus_tag="VQU28_001041"
     CDS             10831..12504
                     /locus_tag="VQU28_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2476407.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALGDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVILQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            12616..13707
                     /gene="ulaG"
                     /locus_tag="VQU28_001042"
     CDS             12616..13707
                     /gene="ulaG"
                     /locus_tag="VQU28_001042"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HET2476408.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            13821..15797
                     /gene="tkt"
                     /locus_tag="VQU28_001043"
     CDS             13821..15797
                     /gene="tkt"
                     /locus_tag="VQU28_001043"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2476409.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVTDYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDANRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKVVRNLK"
     gene            15914..16213
                     /gene="yajC"
                     /locus_tag="VQU28_001044"
     CDS             15914..16213
                     /gene="yajC"
                     /locus_tag="VQU28_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HET2476410.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVEGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            16260..16688
                     /locus_tag="VQU28_001045"
     CDS             16260..16688
                     /locus_tag="VQU28_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HET2476411.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            16690..17100
                     /locus_tag="VQU28_001046"
     CDS             16690..17100
                     /locus_tag="VQU28_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476412.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            17400..20051
                     /gene="adhE"
                     /locus_tag="VQU28_001047"
                     /gene_synonym="adhC"
     CDS             17400..20051
                     /gene="adhE"
                     /locus_tag="VQU28_001047"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HET2476413.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITEPSMEATSALMNHEGIATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
     gene            complement(21036..21344)
                     /locus_tag="VQU28_001048"
     CDS             complement(21036..21344)
                     /locus_tag="VQU28_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2476414.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
     gene            complement(21341..21649)
                     /locus_tag="VQU28_001049"
     CDS             complement(21341..21649)
                     /locus_tag="VQU28_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2476415.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            complement(21654..22949)
                     /locus_tag="VQU28_001050"
     CDS             complement(21654..22949)
                     /locus_tag="VQU28_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2476416.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTKFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPSAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGIVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            complement(22967..24376)
                     /locus_tag="VQU28_001051"
     CDS             complement(22967..24376)
                     /locus_tag="VQU28_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2476417.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLGLDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSLCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            24534..25262
                     /locus_tag="VQU28_001052"
     CDS             24534..25262
                     /locus_tag="VQU28_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2476418.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            25722..25916
                     /locus_tag="VQU28_001053"
     CDS             25722..25916
                     /locus_tag="VQU28_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2476419.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSDVTLITGKNGTGKSTLL"
     gene            complement(26282..27592)
                     /locus_tag="VQU28_001054"
     CDS             complement(26282..27592)
                     /locus_tag="VQU28_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HET2476420.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNFAITNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYRKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTSIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            27829..28701
                     /locus_tag="VQU28_001055"
     CDS             27829..28701
                     /locus_tag="VQU28_001055"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HET2476421.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT         9002 a         5135 c         5988 g         8654 t
ORIGIN      
        1 catttctttt taccagaatc attcgctaat tcttttttta gggcgattga tttgtacttc
       61 cgtcgcatca atcattaccg tgtcctcaga actgagagga gttcttgaaa tcgtaacacc
      121 actttgaaca agagttactt caacccattg gctccgacgg attaagttgc tttcgtggat
      181 accaaaatca gccgcaattt cttcataagt gcggtattct cgcacatatt gaagagtgcc
      241 cataagaagg tcttctaggc ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg
      301 ataagctgtt tttaatacag ctaacatctc tttaaaagtc gtgcgctgaa caccaacaag
      361 acgcttaaat tgtgcattgg ttagttgttt actttcttca taaattttat tgtatcatat
      421 ttttgtttca caggaagtct aataaataaa aattctttaa aattttgttg ggaaaatggg
      481 aaaagatata aatggaaact tagtataaca cttggaaaac tctgtaaaaa tagtaagtca
      541 attcatgtcc cgttttttct ctagggaagt tgggcggcta tatttatatg accatttgtc
      601 gtaataaaca tccctaccaa gggtcaatag aaataaattc aacgaaactt ttgctgtcag
      661 atttagatgc ccaatttgtc atttatactc tttcatatag gactttctat aaggatggaa
      721 aaaagaaggg aaatttgata agataggaat atggattttg aaaaaattga acaagcttat
      781 acctatttac tagagaatgt ccaagtcatc caaagtgatt tggcgaccaa cttttatgac
      841 gccttggtgg agcaaaacag catctatctg gatggtgaaa ctgagctaaa ccaggtcaaa
      901 gacaacaatc aggcccttaa gcgtttagca ctacgcaaag aagaatggct caagacctac
      961 cagtttctct tgatgaaggc tgggcaaaca gaacccttgc aggccaatca ccagtttaca
     1021 ccggatgcta ttgctttgct tttggtgttt attgtggaag agttgtttaa agaggaggaa
     1081 attactatcc tcgaaatggg ttctgggatg ggaattctag gcgctacttt cttgatctcg
     1141 cttgataaaa aggtggatta cttgggaatg gaagtggatg atttgctgat tgatctggca
     1201 gctagcatgg cagatgtaat tggtttgcag gctggctttg tccaaggaga tgccgttcgt
     1261 ccacaaatgc tcaaagaaag cgatgtggtc atcagtgact tgcctgttgg ctattatcct
     1321 gatgatgccg ttgcgtcgcg ccatcaagtt gcttctagcc aagaacatac ttacgcccat
     1381 cacttgctca tggaacaagg gcttaagtac ctcaagtcag acggatacgc tatttttcta
     1441 gctccgagtg atttgttgac cagtcctcaa agtgatttgt taaaagaatg gctgaaagaa
     1501 gaggcgagtc tggttgctat gattagtctg cctgaaaatc tctttgctaa tgctaaacaa
     1561 tctaagacta tttttatcct acagaagaaa agtgaaatag cagtggagcc ttttgtttat
     1621 ccacttgcta gcttgcaaga tgcaagtgtt ttaatgaaat ttaaagaaaa ttttcaaaaa
     1681 tggactcaag gtactgaaat ataaaataga ttttgttata atagttgaaa acgcttaaaa
     1741 aggggtatca tgttatgaca aaaacaattg caatcaatgc aggaagttca agtttgaaat
     1801 ggcaattata cttaatgcca gaggaaaaag tgttagccaa aggtttgatt gaacgtatcg
     1861 gtttgaaaga ttcaatttca actgtaaaat ttgacggccg ttctgaacaa caaattttgg
     1921 atatcgaaaa tcatacacaa gctgttaaaa ttttattgga tgacttgatt cgtttcgata
     1981 ttatcaaggc ttatgacgag attacaggtg ttggccaccg tgttgtagca ggtggagaat
     2041 atttcaaaga atcaacagtt gttgagggag atgttttaga aaaagttgaa gagttgagtt
     2101 tgttggctcc tctacacaac ccggccaatg cagcaggtgt tcgtgctttc aaggaattgt
     2161 tgccagacat taccagtgta gttgtttttg atacttcctt ccacacaagt atgccagaga
     2221 aagcttatcg ctaccctcta ccaacaaaat attacacaga aaacaaggtt cgtaaatacg
     2281 gtgctcatgg tacaagtcac cagtttgtag caggagaagc tgcaaaactc ttgggacgtc
     2341 cattagaaga cttgaagtta attacctgtc atattggtaa cggaggctca attacagctg
     2401 tgaaagccgg caaatctgta gacacttcta tggggttcac tcctcttggt ggtattatga
     2461 tgggaacgcg tacaggggat attgatccag ctatcattcc ttatttaatg caatatacag
     2521 aggattttaa cacaccagaa gatatcagtc gtgttcttaa ccgtgaatca ggtcttttgg
     2581 gagtttctgc taattctagc gatatgcgcg atatagaagc agctgtagca gaagggaatc
     2641 acgaggctag cttggcttat gaaatgtatg ttgaccgtat ccaaaaacat atcggtcagt
     2701 accttgcagt gctaaatgga acagatgcca ttgttttcac agcaggtgtc ggtgaaaatg
     2761 cagagaattt ccgtcgtgat gtaatctcag ggatttcgtg gtttggttgt gatgttgatg
     2821 atgaaaagaa tgtctttggc gttacaggag acatctcaac agaggcagct aaaatccgtg
     2881 tcttggttat tccaacagat gaagaattag tcattgcccg tgacgttgaa cgcttgaaaa
     2941 aataagtgaa actaaaaaaa tattcaatac aaggagttgg gaaagttatt tttccagctt
     3001 ctttttctga tgaaattgtc caaaaccttg ctatgattgg cttttttgaa aaatatggta
     3061 taatagtagt aatttaatag atggagttga gttttgaaga aaaactttcg tgtaaaaaga
     3121 gagaaagatt ttaaggcgat tttcaaggag gggacaagtt ttgctaatcg caaatttgtg
     3181 gtctaccaat tagaaaacca gaaaaaccat tttcgagtag gtctatcagt tagcaaaaaa
     3241 ctggggaatg ccgtcactag aaatcaaatt aagcgacgga ttcggcatat tatccagaat
     3301 gcaaaaggga gtctggtaga agatgtcgac tttgttgtca ttgctcgaaa aggagtcgaa
     3361 accttgggat acgcagagat ggagaaaaat ctactccatg tattaaaatt atcaaagatt
     3421 taccgggaag gaaatgggag tgaaaaagaa actaaagttg actagtttgc taggactgtc
     3481 tctgttaatc atgacagcct gtgcgactaa tggggtaact agcgatatta cagccgaatc
     3541 ggctgatttt tggagtaaat tggtttactt ctttgcgaaa atcattcgct ttttatcgtt
     3601 tgatattagt atcggagtgg ggattattct ctttacggtc ttgattcgta cagtcctctt
     3661 gccagtcttt caggtgcaaa tggtggcttc taggaaaatg caggaagctc agccacgcat
     3721 taaggcgctt cgagaacaat atccaggtcg agatatggaa agcagaacca aactagagca
     3781 ggaaatgcgt aaagtattta aagaaatggg tgtcagacag tcagattctc tttggccgat
     3841 tttgattcag atgccggtta ttttggcctt gttccaagcc ctatcaagag ttgacttttt
     3901 aaagacaggt catttcttat ggattaacct tggtagtgtg gatacaaccc ttgttcttcc
     3961 gattttagca gcagtattca cctttttaag tacttggttg tccaacaaag ctttgtctga
     4021 gcgaaatggc gctacgactg cgatgatgta tgggattcca gtcttgattt ttatctttgc
     4081 agtttatgcg ccaggtggag tcgccctata ctggacagtg tctaatgctt atcaagtctt
     4141 gcaaacctat ttcttgaata atccattcaa gattatcgca gagcgcgagg ccgtagtaca
     4201 ggcacaaaaa gatttggaaa atagaaaaag aaaagccaag aaaaaggctc agaaaacgaa
     4261 ataaataagg aggaatctgg taatggtagt atttacaggt tcaactgttg aagaagcaat
     4321 ccagaaagga ttgaaagaat tagatattcc aagaatgaag gctcatatca aagtcatttc
     4381 tagggagaaa aaaggctttc ttggtctatt tggtaaaaaa ccagcccaag tggatattga
     4441 agcgattagt gaaacgactg ttgtcaaagc aaatcaacag gtagtaaaag gcgttccgaa
     4501 aaaaatcaat gatttgaacg agcctgtgaa gacggttagt gaagaaaccg ttgaccttgg
     4561 tcatgtggtt gatgctatta aaaaaataga ggaagaaggt caaggtattt ctgatgaagt
     4621 caaggctgaa atcttaaaac atgaaagaca tgccagcact atcttagaag aaactggtca
     4681 cattgagatt ttaaatgaac ttcaaatcga ggaagcgatg agggaagaag caggcgctga
     4741 tgaccttgaa actgagcaag atcaaactga aaatcaagac ttgaaagaga tgggcttgaa
     4801 ggtcgagcaa agttatgata ttgcccaggt ggctacggat gtgactgcct atgttcaagc
     4861 gattgtggat gacatggatg ttgaagctac actttcaaat gattataacc gtcgtagcat
     4921 caatctacaa attgacacca acgaaccagg tcgtattatc ggctaccatg gtaaagtctt
     4981 gaaggccttg caactgttgg ctcaaaatta tctttacaac cgctattcca aaaccttcta
     5041 cgttacaatc aatgtcaatg attatgtcga acaccgtgca gaagtcttgc agacctatgc
     5101 gcaaaaattg gcgattcgtg ttttagaaga aggacgtagt catcaaacag atccaatgtc
     5161 gaatagtgaa cgcaagatta tccaccgtat tatttcacgt atggatggcg tgaccagtta
     5221 ctctgaaggt gatgagccaa atcgctatgt cgttgtagat acagaataag taaaatcagg
     5281 gttgacctga ttttttgcta gctagaggag gttaaactaa tgttgaataa gataagagac
     5341 tatttagact ttgctggttt gcagtaccgt aatcctgata aagcgggagc agagcgagag
     5401 aagatgctgg cattccgcca caaaggacaa gaggcccgaa aggcttttac agaactggcc
     5461 aaagcctttc aagcaagcca tccaggatgg caactccaac agactagtca gtggatgaat
     5521 caggcccagc gtttgagacc acatttttgg gtttatctac agagagacga acaagtgaca
     5581 gaacctatga tggccttacg tttgtatggg acatctactg actttggaat ttctttggaa
     5641 gtcagtttca tcgaacgtaa gaaggatgag caaacactgg gcaagcaggc caaagtttta
     5701 gacattccaa ccgttaaagg gatttattat ctaacctact ctaatggtca aagtcaacgg
     5761 tgggaggcga atgaagaaaa gcgtcgtact ttacgcgaga aggtgagaag tcaagaagtt
     5821 cgaaaagttt tagtgaaggt agatgttcct atgacagaaa attcgtctga agaagaaatc
     5881 gtagaaggct tattgaagtc ttattctaaa attcttccct attatctagc tacgagaaaa
     5941 taagataatt tgtaaaacat cataaatcat acagtccaag agtgaacagt ccgctgtgta
     6001 attcttggtc tttttgtttg cgctttcgca ttatataata aacttacaaa aacaattcaa
     6061 aaggagaaca attatggaag tcgtttcaag tgttctaaat tggttttcta gcaatatttt
     6121 gcagaatccc gcatttttcg taggtttatt ggtgttgata ggatatgcac ttttgaaaaa
     6181 acctgcccat gacgtttttt cagggtttgt taaagcaaca gtagggtata tgttgcttaa
     6241 cgtgggtgct ggtggtttgg ttacaacctt tcgtccaatc ttagcagctc ttaactacaa
     6301 attccaaatt ggtgcagcgg ttatcgaccc ttactttgga cttgctgcag caaacaacaa
     6361 aattgcagca gagtttccag attttgttgg aactgcaact acagctctat tgattggttt
     6421 tggaataaat atcttgctcg tagctcttcg aaagattacg aaggtaagaa ccctctttat
     6481 tactggtcac atcatggtac aacaagctgc aacagtatct cttatggttc tattcttagt
     6541 accacaattg cgcaatgctt acggtacagc agcgattggt atcatctgtg gactttactg
     6601 ggcagttagt tcaaatatga ctgttgaggc aactcaacgc ttgactggtg gtggcggatt
     6661 tgcgattggt caccaacagc aatttgcaat ctggtttgta gataaagtag caggacgctt
     6721 tggtaagaaa gaagaaagtt tagacaatct taaattacct aagttcctct caatcttcca
     6781 cgatacagtt gttgcatctg ctaccttgat gctcgtattc ttcggagcca ttcttttaat
     6841 cttgggtcca gacattatgt ctaataaaga agtcatcact tcaggaactc tattcaatcc
     6901 tgctaaacaa gatttcttta tgtacattat ccaaacagcc tttaccttct cagtttactt
     6961 gttcgttttg atgcaaggtg tccgaatgtt cgtatctgag ttgacaaacg ccttccaagg
     7021 tatttcaaac aaattgttgc caggttcatt cccagcggtt gacgttgcag cttcttatgg
     7081 atttggttct ccaaatgctg tcttgtcagg atttaccttt ggtttgattg gtcaattgat
     7141 tacaattgtt ttgctcatcg tctttaaaaa tccgattctt attattacag gatttgtacc
     7201 agtgttcttt gacaatgcag ccattgcggt ctacgctgat aaacgcggcg gatggaaagc
     7261 ggctgttatc ctttccttta tatcaggtgt ccttcaagtt gctctaggag ctctttgtgt
     7321 ggcccttctc gatttggcat cttatggtgg ctaccatgga aatatcgact ttgaattccc
     7381 atggcttgga tttggatata tcttcaaata ccttggtatt gttggttatg tacttgtgtg
     7441 tctcttcttg cttgttattc ctcaacttca atttgccaaa gcaaaagata aagagaaata
     7501 ttacaacggt gaagttcaag aagaagctta gtatctagaa aaggagaaat aaaatggtta
     7561 aagtattagc agcgtgcgga aatggaatgg gttcatcaat ggttatcaag atgaaggttg
     7621 aaaatgctct ccgtaagctt aatcaaacag attttacagt caattcatgc agtgtcggtg
     7681 aagctaaagg tttagcagta ggatatgaca ttgtaatcgc ttctcttcat ttgattcaag
     7741 aattggaagg gcgaactaat gggaagttaa ttgggcttga taacttgatg gatgataaag
     7801 aaatcaccga aaaactcagt caagcactac agtaaaaggt tggagggggc tggacagaaa
     7861 ctgagagtta tcgtttctgt ccagccccct ctttaaataa aggaggcaga tatgaattta
     7921 aaacaagctt taattgacaa tgactcgatc cgactaggtt tagaggctaa caattggaaa
     7981 gaagcagtca aggtagcagt agatcccttg attgaaagtg gggcaatttt gccagagtat
     8041 tacgatgcta tcattgaatc gactgaagag tatgggcctt actatatctt gatgccaggt
     8101 atggctatgc cccacgctag acctgaagct ggtgtgcaaa gtgatgcctt ttcattgatt
     8161 accttacaaa atcctgttgt attttcagat gggaaagagg tatctgtttt gttggcacta
     8221 gcagcaacaa gttcaaaaat tcacacaagt gtagccattc cacaaattat tgccctgttt
     8281 gaattagaag attctattgc acgtttacag gcttgccaga ctaaagaaga tgtcttggct
     8341 atgattgaag aatctaagga tagcccttat ctcgaaggat tggatttgga aagttagaaa
     8401 gaggaataaa gaaatgacaa aaagaatacc taatttacaa gttgcattag accattcaga
     8461 cttgcaagga gcgattaaag cagctgtttc tgttggtcag gaagtagata ttatcgaagc
     8521 tggaactgtt tgcttgcttc aagttggaag tgaactggct gaagtcttgc gtagcctttt
     8581 cccagataag attattgtgg cagacacaaa atgtgctgat gctggtggaa cagttgctaa
     8641 aaataatgcg gttcgtggag cagactggat gacttgtatc tgttgtgcaa ccatccctac
     8701 tatggaagca gctctaaagg ctatcaagac tgaacgagga gaacgaggcg aaatccagat
     8761 cgagctttat ggcgattgga cttttgaaca agctcagctt tggctagatg caggtatctc
     8821 acaagctatt tatcaccaat ctcgtgatgc tcttcttgct ggtgaaactt ggggtgaaaa
     8881 agaccttaat aaggttaaaa aactcattga catgggcttc cgtgtatctg taacaggtgg
     8941 tctagatgta gatactctca aactctttga aggtgttgat gtctttacct ttatcgcagg
     9001 tcgtggaatt acagaggctg cggatccagc aggagcagcg cgtgccttca aggatgaaat
     9061 caaacgaatt tgggggtaaa tcatggtacg tccaattgga atttatgaaa aggcaacccc
     9121 aacacacttt acttggctag aacgtttaaa ttttgccaag gagttaggct ttgattttgt
     9181 cgagatgtct attgacgaac gtgacgagcg tttagcaaga cttgactgga gtaaggaaga
     9241 acgcttggaa gttgtcaaag aaatctatga aactggtgtt cgtattcctt ctatctgttt
     9301 ttcaggccat cgtcgctacc cattgggttc aaaagatcca gttctagagg aaaaatctct
     9361 agaactcatg aaaaaatgta tcgaattagc tcaagacttg ggagttcgta cgattcaatt
     9421 agctggttac gatgtttact atgaggaaaa gtcaccccag acacgccaac gttttatcaa
     9481 aaatttgaga aaagcctgtg actgggctga agaagctcag gtggtacttg ctattgaaat
     9541 tatggatgat cctttcatca atagcatcga aaaatatttg gctatagaaa aagagattga
     9601 ctctcccttc ctctttgtat atccagatat tggtaatgtg tctgcatggc ataatgatat
     9661 ctatagtgag ttttatcttg gtcatcatgc catcgcagct ctccatctca aggatactta
     9721 tgcagtgaca gaaagttcaa ggggccagtt ccgagatgta ccttttgggc aaggttgtgt
     9781 caaatgggaa gaagctttcg atattttaaa ggaaaccaat tataatggac ctttcctaat
     9841 cgaaatgtgg tctgaaaatt gtgaaacagt agaagaaaca cgcgcagcca ttcaagaggc
     9901 gcaagctttt ctctatccac tcattaagaa agcaggtttg atgtaagatg aatcaagtaa
     9961 tcaatgctat gcgtaaacga gtctgtgatg ccaatcaatc attgccaaaa catggacttg
    10021 tcaaatttac ctgggggaat gtatctgaag tcaatcgcga actcggtgtc attgttatca
    10081 aaccatcagg cgtggattat gacgaattga cacctgaaaa catggtagtg actgatctag
    10141 atggtaagat cctagaaggg gatttaagac catcttccga cctcccaact catgtgcaat
    10201 tatataaggc ttggtcagaa attggtagtg tggttcacac ccattcgaca gaagctgttg
    10261 cttgggctca ggcaggtcgt gatattcctt tctacggaac aacccatgca gattatttct
    10321 acggttcaat cccttgcgcc cgtagtttga ccaaggacga agtagaagtg gcctatgaaa
    10381 aagatactgg cctggttatc gtagaagagt ttgaacatcg cggacttaac ccggttgaag
    10441 taccaggaat tgttgtacgc aatcacggtc cattcacctg gggcaaaaat ccagagaatg
    10501 ctgtttatca ctctgtcgta ctagaggaag tatcaaagat gaatcgcttt acagaacaaa
    10561 tcaatccaag agttggacct gctccccagt acatactaga aaaacactac caacgtaaac
    10621 atggaccaaa tgcttattat ggtcaaaagt aagaacgatg aaggaggaga aaaagataaa
    10681 tttagctcct ctttttacat ttgattttta ttgagagtaa agttggagtt gaagtaattt
    10741 taaaagattt tttagaaata gcgcttgata tatatatggt aaaataaaaa gaattgctgt
    10801 gatatcaata gatttggggg attttttaat atggtactgg ataaggcaag ttgtgatttg
    10861 cttcaatatt tgatggatca agaaacgtcc aaaacgatta tggcgatttc gaaagatttg
    10921 aaagagtcaa gaaggaaaat ttattatcac attgacaaaa tcaatgctgc tctgggtgac
    10981 gaggcgcttc acatcattag tattccacga attggtattc acttaacgga agagcagaga
    11041 gatgcttgtt gtaaactatt atcggaagta gattcgtacg attatatcat gagtgcgcat
    11101 gaacgtatga tgataatgtt actatggata ggtatttcta aagaacgtat tacgattgaa
    11161 aaattgatag agttaacaga ggtatctagg aatactgttc tcaatgattt gaatagtatt
    11221 cgttatcaac taactttgga acaatatcag gtgatcttgc aagtgagcaa gtcacaggga
    11281 taccaccttc atgcccaccc tcttaataaa attcagtatc ttcaatcgct tctatatcat
    11341 atttttatgg aagaaaatgc cacttttgta tctattttag aagataagat gaaagagagg
    11401 ttagatgatg agtgtttgct ttctgttgaa atgaaccaat tttttaagga acaggttcct
    11461 ttagttgaac aagatttagg gaagaaaata aaccatcatg aaataacttt tatgttgcag
    11521 gttctacctt atttgctgtt aagctgtcat aatgttgaac agtatcaaga aagacatcag
    11581 gatatagaga aagaattttc tttgataaga aaaagaatag agtatcaggt gtctaagaaa
    11641 ttaggagaac ggttgtttca aaagtttgaa atttctttgt caggacttga agtttctctt
    11701 gtagctgttc tcctcctctc ctatcgtaaa gatttggata ttcatgcaga aagtgatgat
    11761 tttcggcaat taaaacttgc tttagaagaa tttatctggt attttgaatc acaaatccga
    11821 atggagattg agaacaagga tgatttgtta cgaaatttga tgatccactg taaagccttg
    11881 ttatttagaa agacttacgg tattttttct aaaaatcctc taacaaaaca aattcgatcc
    11941 aagtatggag aattattttt agtcactaga aaatctgcgg aaattttaga aggagcatgg
    12001 tttattcggc taacagacga tgatattgcc tatttgacga ttcatattgg aggattttta
    12061 aaatatacac catcatctca aaaaaatatg aaaaaagttt atctcgtttg tgatgaaggt
    12121 gttgcggttt cgagactttt gctgaaacaa tgcaaacttt attttccaaa tgagcaaatt
    12181 gacactgtat ttacaacaga acaatttaag agtgtggaag atattgcaca agttgatgta
    12241 gtgattacta ctaatgatga tttggatagc agatttccga ttttaagggt taatcctatc
    12301 cttgaagcag aagatatttt gaaaatgcta gactatctta aacacaatat atttcgtaat
    12361 aagagcaaaa gtttcagtga aaatctttct agtcttattt cgtcttatat tgtagacagc
    12421 aagttggcta gtaagttcca agaagaggtt caaacactta taaatcaaga aatagtagtt
    12481 caagcttttt tggaagatat ttgaaggaca gtccaatgat gaacacaaac ctgtgttttt
    12541 cttggtcttt tttagtgttt tgaagggtgg tatactaatc tcaaagataa caattatatc
    12601 caaaggaggc aacatatgcc aaacgtcaaa gaaattacaa gagagtcatg gattttagcc
    12661 actttcccag agtggggaac atggttgaac gaagaaatcg aagaagaagt cgtacctgaa
    12721 ggcaactttg ccatgtggtg gctaggcaac tgtggtactt ggattaagac accagctggt
    12781 gctaacgttg tcatggacct ttggtcaaac cgtggaaaat caaccaaaaa agtgaaagat
    12841 atggttcgtg ggcaccaaat ggcaaatatg gcaggtgttc gtaagctgca accaaacttg
    12901 cgtgttcagc caatggttat cgatccattt gctatcaacg aactagacta ttacttagtt
    12961 tcacacttcc acagtgatca tatcgaccca tacacagctg cagcaattct caataatcct
    13021 aagttagagc atgttaagtt tatcggtcct taccactgtg gacgaatctg ggaaggatgg
    13081 ggtgttccaa aagaacgtat catcgttgtt aaaccaggtg acactatcga attaaaagat
    13141 atgaagattc atgcagtaga atcatttgac cgtacttgct tggtaactct cccagtgaac
    13201 ggtgctgatg agacaggcgg tgaacttgct ggcttggctg ttacagatga agaaatggct
    13261 caaaaggctg ttaactatat ctttgaaaca ccaggtggaa ccatctatca tggtgcagat
    13321 tctcacttct caaactattt tgcaaaacat ggtaaagact ttaaaattga tgttgctttg
    13381 aataactatg gtgaaaatcc ggtaggtatc caagacaaaa tgacatctat cgaccttctt
    13441 cgtatggcag aaaatctgcg taccaaagtc attatcccag ttcactatga tatctggtct
    13501 aacttcatgg cttctactaa tgagattcta gaactttgga aaatgcgaaa agatcgcttg
    13561 caatacgatt tccatccatt tatctgggaa gttggcggta agtacactta tcctcaagat
    13621 caacacttag tagaatacca tcatccacgt ggttttgatg attgttttga acaagactct
    13681 aacattcaat ttaaagcttt gctataatat aaaaatattc tttggaggtt atccgtatgt
    13741 tgcatgatac ggataatttt catataatag taaaatagaa tgtgtgattc aataatcacc
    13801 tcaaatagaa aggaaattct atgtcaaatc tatctgttaa tgcaattcgt tttctaggta
    13861 ttgacgccat taataaagcc aactcaggtc atccaggtgt ggttatggga gcggctccga
    13921 tggcttacag cctctttaca aaacaacttc atatcaatcc agctcaacca aactggatta
    13981 accgcgaccg ctttattctt tcagcaggtc atggttcaat gctcctttat gctcttcttc
    14041 acctttctgg ttttgaagat gtcagcatgg atgagattaa gagtttccgt caatggggtt
    14101 caaaaacacc aggtcaccca gaatttggtc atacggcagg gattgatgct acgacaggtc
    14161 ctctagggca agggatttca actgctactg gttttgccca agcagaacgt ttcttggcag
    14221 ccaaatataa ccgtgaaggt tacaatatct ttgaccacta tacttacgtt atctgtggag
    14281 acggagactt gatggaaggt gtctcaagcg aggcagcttc atacgcaggc ttgcaaaaac
    14341 ttgataagtt ggttgttctt tatgattcaa atgatatcaa cttggatggt gagacaaagg
    14401 attcctttac agaaagtgtt cgtgaccgtt acaatgccta cggttggcat actgccttgg
    14461 ttgaaaatgg aacagacttg gaagccatcc atgctgctat cgaaacagca aaagcttcag
    14521 gcaagccatc tttgattgaa gtgaagacgg ttattggata cggttctcca aacaaacaag
    14581 gaactaatgc tgtacacggc gcccctcttg gagcagatga aactgcatca actcgtcaag
    14641 ccctcggttg ggactacgaa ccatttgaaa ttccagaaca agtatatgct gatttcaaag
    14701 aacatgttgc agaccgtggc gcatcagctt atcaagcttg gactaaatta gttacagatt
    14761 ataaagaagc tcatccagaa ctggctgcag aagtagaagc catcatcgac ggacgtgatc
    14821 cagtcgaagt gactccagca gacttcccag ctttagaaaa tggtttttct caagcaactc
    14881 gtaattcaag tcaagatgcc ttgaatgttg tggcagctaa attaccaacc tttctaggtg
    14941 gatcagctga ccttgctcac tcaaacatga cttatatcaa gacggatgga cttcaagacg
    15001 atgccaatcg cttgaatcgt aacattcagt ttggtgttcg tgaatttgca atgggaacaa
    15061 tcttgaacgg gatggccctt catggtggac ttcgtgtata cggtggaact ttcttcgtct
    15121 tctctgacta tgtgaaggca gctgtccgct tgtcagcctt acaaggactt cctgtgactt
    15181 atgtctttac ccatgattca atcgcagttg gggaagatgg tccgactcat gaaccagttg
    15241 agcatttagc aggtcttcgt gctatgccaa atctaaatgt tttccgtcca gcagatgcgc
    15301 gtgaaacgca agcagcttgg taccttgcag tgacaagtga gaaaacacca actgcccttg
    15361 tcttgacacg tcaaaatttg actgttgaag atggaacaga cttcgacaag gttgctaaag
    15421 gtgcttatgt tgtatatgaa aatgcagccg actttgatac catcttgatt gcgacaggtt
    15481 cagaggttaa tcttgctgtc tcagctgcta aagaattggc tagtcaaggc gaaaaaatcc
    15541 gcgtagtcag catgccatct acagatgtct ttgataaaca agatgcagct tacaaggaag
    15601 aaattcttcc aaatgcagtc cgccgtcgtg ttgcagtcga aatgggtgca agtcaaaact
    15661 ggtacaaata tgttggtctc gatggtgccg ttctaggtat tgataccttc ggagcctctg
    15721 ccccagcacc aaaagtattg gcagaatatg gctttactgt agaaaatctt gtaaaagttg
    15781 ttcgaaactt gaaataatcc taaaaatcag ggcgtaagct ctggtttttc ttaccagaaa
    15841 agtaaggtac aatcttgtaa aagtagctga aatttgatat agtagtccta tgtaaaagac
    15901 aaaggagaaa acaatgaatc caaatattac ttttttaatc atgcttgtag gtatgatggc
    15961 cttgatgttc tttatgcaac gttctcaaaa gaaacaagct caaaagcgta tggaaagctt
    16021 aaataaacta caaaaaggct atgaagtgat tacaatcggt ggactttatg gaacagtcga
    16081 tgaagtagat acggagaagg gaacaatagt tcttgacgta gagggagttt acttgacttt
    16141 tgaactagct gctatcaaga cagtattacc gctgaaagaa acagcttcac tagaaggcgc
    16201 aattgaaaaa taagacggga tcacgaactc ccgtttttct ataaaagaaa ggaaatggga
    16261 tgaaaaaatt agtctttgtc tgtctgggaa atatttgccg tagccctatg gccgagtttg
    16321 ttatgaaatc aatgacagat aactacgaaa tccaaagtcg agcaacttcc tcttgggaac
    16381 atggcaatcc gattcataag gggactcagg gaatttttca agagtatgag attccttatg
    16441 acaagaacaa gacatcgctt cagattagta aggaagattt tgaagccttt gattatatta
    16501 tcggaatgga cgcttcaaat atttctgact tacgtcagat gtgtccagta gactgtcaag
    16561 ataagattta ctcattttca tctgaaagtg ttccagaccc ttggtataca ggagattttg
    16621 aagaaaccta tcgacgtgtt caagagggct gtcaagcttg gttagaacgt ttagaaaagg
    16681 agagttaagg tggaaaatct tacgaatttt tacgaaaagt atcgtgtcta tctgactcgt
    16741 ccacgtttag agcttttggc agtagttacc attgttttct gtgctgtact cgtctttttt
    16801 ctaaatattc caggaaaagg tgtcttaaaa ctcgataatg gaacgattgt ttatgatggc
    16861 agtcttgtcc gcggtaaaat gaatggccaa ggtaccatta ccttccaaaa tggagaccaa
    16921 tatacaggtg gcttcaacaa tggagccttc aacggaaaag gtacctttca atctaaagaa
    16981 ggctggacct acgaaggtga ttttgtaaat ggtcaggctg aaggaaaagg gaaactaaca
    17041 acagaacaag aagtcgttta tgaaggaact tttaaacaag gcgtttttca acaaaaataa
    17101 agcctcctta tcaaaggagg tattattaga atcgcaaggt aagcgcttac ctgcaaatcc
    17161 ctttctttcc aaatccctct tccaagcaag tttgtgaaat aaaaaatatt tgaaataaat
    17221 ttcacaaact tcaaagataa aacctgataa gaaaagaaaa tgagaaaagt ttcacaagag
    17281 tttaaaaatt ctttgtgaaa tgtttatgaa actgtttaca aagttgaaat aatgcgttat
    17341 aataaacttg tgaaaaaatt aacaaaggat atcgttcctt gaaagctatg gaggaaaata
    17401 tggctgataa aaaaactgtg acaccagagg aaaagaaact cgttgctgaa aaacacgtag
    17461 atgagttggt tcaaaaagct ctagttgccc ttgaagaaat gcgtaaattg gatcaagaac
    17521 aagttgacta catcgttgcc aaagcatcag tagcagcttt ggatgcccac ggagaattgg
    17581 ctttacatgc ctttgaagaa acaggacgtg gtgtatttga agataaagca actaagaact
    17641 tgtttgcttg tgaacacgtg gtaaacaaca tgcgtcacac taaaacagtt ggcgttatcg
    17701 aagaagatga tgtaacagga ttgaccctga ttgctgaacc agttggtgtt gtttgtggta
    17761 tcactccaac aacaaaccca acatcaacag cgatcttcaa atcattgatt tcattgaaga
    17821 cacgtaatcc aatcgtcttt gccttccatc catcagctca agaatcatca gctcatgcag
    17881 ctcgtatcgt ccgcgatgca gctatcgcag ctggtgctcc tgaaaactgt gtacagtgga
    17941 tcactgaacc atctatggaa gcaactagtg cccttatgaa ccacgaaggt attgcaacaa
    18001 tccttgcaac aggtggtaat gccatggtta aagccgcata ctcatgtggt aaaccagctc
    18061 ttggggtagg tgccggaaac gttccagctt atgttgaaaa atcagcaaac attcgtcaag
    18121 cagcacacga tatcgtcatg tctaaatcat ttgataacgg tatggtctgt gcatctgaac
    18181 aagcagttat cattgataaa gaaatttacg atgaatttgt agcagagttc aaatcttacc
    18241 acacttactt tgtaaacaaa aaagaaaaag ctcttcttga agagttctgc ttcggcgtca
    18301 aagcaaacag caaaaactgt gctggtgcaa aattgaacgc tgacatcgtt ggtaaaccag
    18361 caacttggat tgcagaacaa gcaggattta cagttccaga aggaacaaac attcttgctg
    18421 cagaatgtaa agaagttggc gaaaatgagc cattgactcg tgaaaaattg tcaccagtta
    18481 ttgcagtttt gaaatctgaa agccgtgaag atggtattac taaggctcgt caaatggttg
    18541 aatttaacgg tcttggacac tcagcagcta tccacacagc tgacgaagaa ttgactaaag
    18601 aatttggtaa agctgttaaa gctattcgtg ttatctgtaa ctcaccttct acttttggtg
    18661 gtatcgggga cgtttacaat gccttcttgc catcattgac acttggatgt ggttcttacg
    18721 gacgcaactc agttggggat aacgttagtg ccattaacct cttgaatatc aaaaaagtcg
    18781 gaagacggag aaataacatg caatggatga aacttccttc aaaaacatac tttgaacgtg
    18841 attcaattca ataccttcaa aaatgtcgtg acgttgaacg tgtcatgatc gttactgacc
    18901 atgccatggt agagcttggt ttccttgatc gtatcatcga acaactggac cttcgtcgca
    18961 ataaggttgt ttaccaaatc tttgcggatg tagaaccgga tccagatatc acaactgtaa
    19021 accgtggtac tgagattatg cgtgccttca aaccagatac catcatcgca ctcggtggtg
    19081 ggtctccaat ggatgctgcc aaagtaatgt ggctcttcta cgagcaacca gaagtggact
    19141 tccgtgacct tgtccaaaaa ttcatggata tccgtaaacg tgccttcaag ttcccattgc
    19201 ttggtaagaa gactaaattc atcgcgattc caactacatc tggtacagga tctgaagtaa
    19261 caccatttgc cgttatctct gataaagcaa acaaccgtaa atacccaatc gctgactact
    19321 cattgacacc aactgtggca atcgtagatc ctgctttggt attgacagtt ccaggatttg
    19381 ttgctgctga tactggtatg gacgtattga ctcacgcgac agaagcatac gtatcacaaa
    19441 tggctagtga ctacactgat ggtttagcac ttcaagccat taaattggtc tttgaaaatc
    19501 tcgaaagctc agttaagaat gcagacttcc actcacgtga gaaaatgcat aacgcttcaa
    19561 caatcgctgg tatggccttt gccaatgcct tcctaggtat ttctcactca atggcccata
    19621 agattggtgc gcaattccac acaatccacg gtcgtacaaa tgctatcttg cttccatacg
    19681 ttatccgtta caacggtaca cgtccagcta agacagcaac atggcctaag tacaactact
    19741 accgtgcaga tgaaaaatac caagatatcg cacgcatgct tggacttcca gcttctactc
    19801 cagaagaagg ggttgaatct tacgcaaaag ctgtctacga actcggtgaa cgtattggga
    19861 tccaaatgaa ttttagagac caaggaattg acgaaaaaga atggaaagaa cattctcgtg
    19921 aattagcctt cctggcttat gaagaccaat gttcaccagc taacccacgt cttccaatgg
    19981 tagaccatat gcaagaaatc atcgaagatg catactatgg ctacaaagaa agaccaggac
    20041 gccgtaaata attgtttatc agtctagaag caagacaaaa actcaatttg agggaaagat
    20101 ccagtaattt ttctatgata aaaggcatcc tatcaaggtt tttgaacacc tgataggatg
    20161 cctttttatg atattgaggc ctttttgccc tttttgaaaa actagaatag aaacaaaata
    20221 tataatagat tgaaactgga atagtacact tctgcttcta aaacattgtt agaattcgat
    20281 ttgactgtcc tgatcgattt gtcctattcc tatttcattt tactatataa aaaatatagt
    20341 atagtagact gaatctaaaa tagtacgaaa caattgctaa aacatttata gaaattaatt
    20401 ttactttcct gatagagttg ttcacatctt atttcaattc actatagttt aatttaagag
    20461 tagtatttac taaggcccaa ttaaaatcaa agagcaaact agaaaacgag tgccattcag
    20521 ctcaaaacac tgatttgaga ttgcagataa gactagcccc tcattaacag gtttacgata
    20581 aaacgatgac aaggtgtgtt gctttttgat ttctaaagag tataatgata gatctctata
    20641 aaataagtgc gaaggaaatg agcttttata gtcctttcgt tttaaaatac tatctcagat
    20701 gttcttatat cgacaagaag tttttgagtc attccctcat catacatatt aaataaatag
    20761 tggctcattc aattttttac tagaataata agctagtata gtaaactgaa ataagatata
    20821 aacaaataaa ttggagctta acatctattt ccagcaattt tttagaaact gcagtggact
    20881 attctaaatt caacacatta taaaaactag agtaaaagaa aaggattgga tcttgtgtaa
    20941 tgcaggatct aatcctttca atcattttgt ccaacttttg gaggttccta caatgtagcc
    21001 gtcattaata aagacagatg ggaacgacag tgttcctatt tattttgata gagatcgatg
    21061 aattctttag atagcaactg aataatctct gttgaagcca tttggtcttc tgcatgcata
    21121 aatagcaagg agaatcctat tttttctcca gtagcttctt tttgtatgag attagagtga
    21181 atcttgtgcg cttctactaa ggagtcttcc gcttcttcaa ctttaatttt cgcttctttt
    21241 aaatttcctg ccttagctag ttggatggct tcaataaagg atgatttggc tgctccacta
    21301 ttggcaatga gctgaaaaca gatatattcc atttcttctg tcatcttatt tctcctatcc
    21361 atgcaagtgc ttgttccaga acttttgctc cattcatcat tccgtaatcc cgcatatcaa
    21421 tggtatctac agggatattt cctgcaattt ctttcacagc aagtaactca taacgaattt
    21481 gtggcccaat tagaatgaca tctgcttcat ggatattctt tttcgcttct gtcattgatt
    21541 ttgcttggat agaaatttca atcccacgtt cagtcgcact ttgttgcatt tttttaacaa
    21601 gcatacttgt cgacattccc gcattacata ctaataaaat ttgtttcata atcttaacct
    21661 tccatttctt gctcaacaac tttgtcatta actttgataa atggaatgta tagaagaact
    21721 ccaagtgcaa agatgatgaa ttgaactaga actgctctca cgtcccctgc tgttgctaac
    21781 catgcattta agaatactgg tgtagtccaa ggaacttgta taaatgcagg actcatgaat
    21841 cctgtaactg ttgctaagta gctgattaaa ataccaagga ctggaactgt gataaatgga
    21901 atagctaatg aaatgttata aacgattggg taaccgaata atactggttc attgatattg
    21961 aagataccag gtccaaaaga taatttagcc acgtttttag aaacagcatt gcgactcact
    22021 aagaatgttg ctattaataa acataatgta gatccactac cacccattaa agcgaatgtt
    22081 tgtatttgtg ataagttgat gatgtgtgga atggcttgtc cattatttgc tgcagtgatg
    22141 ttttcagtaa tgttaattaa tagtaatggt tctaggatgg cactgtaaat aactgcttgg
    22201 tgaataccaa atagccataa catatttcct aaagagtaaa taataatgac cccgattaag
    22261 cttgtaccaa tatgacgaat tggttcttga ataaagattg taatgattga gattaagttc
    22321 attccagtta tattgaataa taatgctgaa acaatcccaa ataaggagat gacagtcatg
    22381 actggaagta atacgctaaa tgatctacta acagctggtg gaatattttc accaaggttc
    22441 atttgtaaag ctttaacgtt tgataattca atgaataatt ctgttgcaat aatcgctacg
    22501 ataaccccgg cgaacattgc gcctgtacct gtgttgttga atgaaagaac acctgaaatg
    22561 tttacagcat cttttgctcc gtcaggaact acagaaactg tatttggcat catcacaatt
    22621 aaagaaacta atgatagcat tgatgctgct gacgggtttt cgaaatctct gtttttagct
    22681 aagaaataac caaccattac agcaataatc atacctgaaa tacttaaagt accgtttgca
    22741 attgttattc cccaatattg gaattttgtt aatgtatccc cttggaaaat ccacttaaat
    22801 accgtgttgt tcaaaagaac gattaaacct gccaaaatat ataatggcat tactgttacg
    22861 aatgcatctc ttagggtttt taaatgaatt tggttcccta gtttaccagc aaaggtggca
    22921 aattgatcta taaattttga gtttttcatc tgaatatctc ctctctttac tcctcaattt
    22981 ctacttcaaa accgttattg tcacttactt tgcgatacca tcttcctgag ctcttgatgg
    23041 ttctcttttg cgtttctaaa tcaacggaga taaatccgta acgattctta tatgcattat
    23101 tccaagacca gcaatcaaat gcggtccaag cgtggtatcc aaaacagaga cttccctctt
    23161 caatggcttt atgtagccat cttaaatgtt cttcataaaa ttcaatacga tacacgtcat
    23221 cgataactcc attttcatcg ataaaccgtg cttcgttttc aacacccatt ccgttctcac
    23281 tgataaacca tgggatatta ccatattctt ccttcacaat catagcaata tcataaatgg
    23341 ctttcggaaa aatttcccaa ccacgatatg gattcatgcg acgctccggc cattcatact
    23401 ctttgaagta ttggtctggc atccagggcg tctgatattc ctcaggattt gcttgtgctt
    23461 gaacacgttt tggatggtag tagtttactc caagaaaatc aaccgtattt gatttcatca
    23521 gttgaagctc tttttcggta tgactccata acacgccatc tctctctagc tgttttacca
    23581 atctttctgg gaaagttcct ttaacagctg gattcaagaa gactttgtta aagaagtctt
    23641 ctgtaaatcg acttgcttct aagtcttctg gagaattact tcttggataa gcaggtgtca
    23701 agtttaaaat aatcccaatc tttccatcaa gtcctaatga gcgatatagt tgaatcactt
    23761 ttgcactagc aaggtttaga ttatagatga cttgtacggc ctcttttccc tttcctttta
    23821 gatttggata atggaaagca tataagtatc ctgcttctgg aatgaccatt ggctcattga
    23881 aagttgtcca gtaatgaacc ttatctccaa agcatgtgaa agcagtcttg gcaaacttca
    23941 cgaataactc cactacatgt ttgctttccc aaccaccgta tttttgaaga agttccactg
    24001 gtaaatcaaa atgatgtaaa ttcatcacaa gatccatctg gttcttttta gcttcttcaa
    24061 tgatggcatt gtagaaagca ataccttttg gatcaggctc acctgtctct aaattcttga
    24121 tgagtcgact ccattggatg gaagtgcgga aagaattaac tccaatttcc ttcatcaagt
    24181 ggaaatcttc ggtgtaagta tgaaaaaagt tactggctac aagaggtccg acattatcga
    24241 aaaaatcttc tggatgcgtt ttgaaccagt agtccatcac attttcatgt acttttccat
    24301 attgaccttc tgtctgaggt ccgctagaag cagctcccca ccaaaacaga ttaggaaata
    24361 ctaacttttt tttcatgctc aacctccttt ctattatgct atacataaag tatagcataa
    24421 ttttaaaatt tgtctagaca tttttaaaaa ttgttgatac atatattgtg tttgcctcat
    24481 ttcttgaact ttgttatact actattagaa tattatagag agaaccagat taaatgagta
    24541 caaaatattt atttatttac aatgagattc gtgaaaagat tctctgtaat aaatatacca
    24601 tgaacgaaca attgcctgat gaaatgacat tagctaaaca gtttgcctgt agtcgaatga
    24661 cgatcaaaaa agctttagac ttgttagttt ctgagggctt aatttttaga aaacgtgggc
    24721 agggaacctt tgttctctct cgtggcagct caaaaagaaa attaatcgtt ccagaaagag
    24781 atatccgggg actgacaaaa atatctgaag atgctcattc tacaattgac tcgaggatta
    24841 ttcacttcaa attagaattt gcaaatgaat ttttagcaga aaaactacag gtcgctttgc
    24901 agagtccagt ttataatatt taccgcctgc gtattattga cggtaaacct tatgttctgg
    24961 aacaaactta tatgagtacc gatgttattc caggtattac tgaagatatt ttacaaaaat
    25021 cgatttacaa ttacattgaa ggaaagttag gattgcatat tgccagtgct acaaaaatct
    25081 tgcgagcttc ttctagttca gaaaatgagc aacattactt gcagctcctt ccaacagaac
    25141 cggtatttga agtagaacaa gtggcttatt tggataacgg aactccgttt gagtactcga
    25201 ttagtcgtca tcgctatgat ttatttgaat ttaattcttt tgcattacga cattcctcct
    25261 aggagaaaat gtgaaaatga agccaatctt ttacaggctc tagtttaaga aaaatttaaa
    25321 acagggcaag aaggtcccat ctatgcttaa atggtttctc ttttctaaat aagatggctt
    25381 taaaagagtg atcgttttat ccatcatgtt gaaaaatatc ttcgtatagc ttatagagta
    25441 ggtactgaaa ttgttcacct gatctacttc ttatagttat ttagttttaa atagtgtttc
    25501 aaacattctt acactgacga gaagtttttg agtcttttct tgtaacacat atagtatact
    25561 gaggttagaa tagtagactg tgacttctaa caaattgcta gaaatgaatt tcaatctccc
    25621 aatttatttg cttatatatt attttaatat attaaataaa ttctggatca taatcattta
    25681 aaaaaatttt atttttttat ttttcattac gaataatata gatgaagggg aaagagtatg
    25741 aaaacagaac tgtttctttt gctattagtt caaaaggaga aaaaatgaaa gtagaaaata
    25801 tttcgtatag ggtggatcat cgtatattgt ttgataatat ttcttttgat acttcgagtt
    25861 cagacgtgac attaattact ggtaaaaatg gtacaggaaa gtcaacttta ctatagtgga
    25921 ttgaaactag aatagtacac atctacttct aaaatattgt tagaaatcga tttgactatc
    25981 ctgatctatt tgtcctgttc ttatttcatt tcactatatc tcaaattgag tatgacgaag
    26041 tgcgctccca tgtcctggga acgcactttc ttcatatttt tcatattctt gaatccatcg
    26101 ataaagacta ttgggatgaa tttttaaagt tgaactaatc atttttacag gatgaggttt
    26161 acagcagaga gtttgaaggc tttatcaaag gtttttcttg gcataatgac ttttcctcgt
    26221 ttccacttaa ttttgtgtct actttattat accaagtcca cccttaagtt agataataaa
    26281 tctaacttaa ggaagctaga aggatgagaa tccaggtggt caagagtccc aaacttaagc
    26341 tgattgggac acccagaata atttgctttt tgaaggcaag gccacgttcc tctatattgg
    26401 gaagtgagag ttgaatgaga gaaccagctg atgaaaaggg tgagatatta gtagatagag
    26461 cgccaataac ggtggctgtt gtgagtaagt gaatatcaat ctggggattt tgagcactga
    26521 tgatagcaat gatgggaaag agggctggag ctacaacgga tagggtggaa ctaaagagtg
    26581 acatcacccc ggctatcaca caaaagaaca gaggtaacca gaaatgagga atggatgttg
    26641 tcatgaggtg ccctatcagt gtgactaaac ctgacttgac cgctagagac attagtaagc
    26701 tcatgccgca gagcatgata attgtagccc agggaacttt agccaaaatg gcttcttgct
    26761 tccctaattt gagccttaag gcgaggcaga ccatgagtat tgagacaaag ccaatatcaa
    26821 atgtttttcg ataagtagct atccaggcga tgtttgggaa aatgagatgc aacaagggaa
    26881 aaagccaaac caaaaccatg ctgctgatca tgagcaaggt ggtttgtctt tgaaccttgc
    26941 tgaggagtgg tggttggtca atagtcaagg atgagtttgt tcttccctta ctatagtgac
    27001 tgtaacagga taataaaagc aagacaatga gtgggtagat aatgctgacg ataaagatat
    27061 gattgccaag tgaaaaagct tgctcttccc atcccatttg cttaaacagg ccttgaaaga
    27121 caatgcctga gctactggtt atcaaattag cccctcctga agctccccaa ttgacggctt
    27181 gagctccaat caaagggtgt ttgtccgctt tttgacagag agtaatcgct agaggacagc
    27241 aaacggccat agtagtgaaa aatccagcac ctaaagcaga caaaagggtt gccatcaggt
    27301 ataaaatcat gtagagggcg ttagggtagg tgcgtgtgcg gtagagaatg tgttgagcca
    27361 aaacatcaag agtaccgtta gttattgcaa agttataaaa gagagagacg ctaaaaatgg
    27421 taaaaaagag tgaggttggc caaaaatgaa gaagttcttt ggggcttaat cccatgagag
    27481 tggttgcgat gaggtaagaa aaagcaatag ccagcaggcc aatattgatt ttggtgcggt
    27541 aaccaattcc aatggctaga gcaatggcgc taatcattat taaatgaatc attgaattgt
    27601 cctttcagtt agaatatgga aataggttgg caattttggc gctcaaaaga ccacctgttt
    27661 cctattggta cttgactatt atacacaaaa aaaatacaat tgtcttgaca attgtattga
    27721 cacttgttag aattaaaaat gaggtattca ggcttgtgat ttcctatgga agttaatagt
    27781 gattgcctct aatgcttaca agtgatatta aaaatagagg acctagtgat gtcaatcatt
    27841 tcaactgatt taaccccttt tcaaatagat gatacattga aagcagcctt gcgagaagat
    27901 gttcattccg aagattacag taccaatgcc atttttgatc atcatggcca agccaaggtg
    27961 tcgctttttg ccaaggaagc tggtgtttta gcggggctaa ccgtttttca aagggttttt
    28021 accctatttg atgccgaggt gaccttccag aatcctcatc aatttaagga tggggatcgt
    28081 ttgactagtg gcgatttggt tttagaaatc ataggctcgg tgagaagtct cttaacatgt
    28141 gaacgcgttg ccttgaattt tttacaacat ttatcaggga ttgcttcgat gacagctgct
    28201 tatgtagaag ccttaggcga tgattgcatt aaggtatttg atactcgaaa aactactcct
    28261 aatttacgtc tttttgagaa atatgcagtg agagttggcg gtggctataa tcatcgcttt
    28321 aatttatcag atgctatcct gctaaaagac aatcacattg cggcagtagg tagtgttcaa
    28381 aaggcaattg ctcaagcgcg tgcctatgcc ccttttgtga aaatggtcga ggtggaagtg
    28441 gaaagccttg ctgctgccga agaagctgcg gcggcgggtg ctgatattat catgttggat
    28501 aatatgtcat tggaacagat tgaacaggcc attaccctaa ttgcaggacg ttctcggatt
    28561 gaatgttctg gaaatattga tatgaccact attagccgtt ttcgtggttt agcgattgat
    28621 tacgtctcca gtggtagttt aacccatagt gctaagagtc ttgatttttc catgaagggg
    28681 ttaacctacc ttgatgtcta agttgtaaaa taaactaact ttttaaagga tgtctttcct
    28741 ctagaacgag ttttatgtca gatagtttaa acgcctctt
//
LOCUS       DAVBCB010000025        28430 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVBCB010000025 DAVBCB010000000
VERSION     DAVBCB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 28430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.025"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQU28_001056"
     CDS             complement(295..2022)
                     /gene="ezrA"
                     /locus_tag="VQU28_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HET2476422.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLEAGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQU28_001057"
     CDS             complement(2104..4050)
                     /gene="gyrB"
                     /locus_tag="VQU28_001057"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HET2476423.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4065..4628)
                     /locus_tag="VQU28_001058"
     CDS             complement(4065..4628)
                     /locus_tag="VQU28_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2476424.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4713..5267)
                     /locus_tag="VQU28_001059"
     CDS             complement(4713..5267)
                     /locus_tag="VQU28_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HET2476425.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5278..6501)
                     /locus_tag="VQU28_001060"
     CDS             complement(5278..6501)
                     /locus_tag="VQU28_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2476426.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6538..8988)
                     /locus_tag="VQU28_001061"
     CDS             complement(6538..8988)
                     /locus_tag="VQU28_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HET2476427.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIHISSTKSGRTLLSSL
                     LPESCQAMGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="VQU28_001062"
     CDS             complement(9187..10116)
                     /locus_tag="VQU28_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2476428.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVK"
     gene            10256..10543
                     /locus_tag="VQU28_001063"
     CDS             10256..10543
                     /locus_tag="VQU28_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HET2476429.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQU28_001064"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="VQU28_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HET2476430.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQU28_001065"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="VQU28_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HET2476431.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="VQU28_001066"
     CDS             complement(12684..15230)
                     /locus_tag="VQU28_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HET2476432.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHIAYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15354..15794)
                     /locus_tag="VQU28_001067"
     CDS             complement(15354..15794)
                     /locus_tag="VQU28_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HET2476433.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQVHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15769..16638)
                     /locus_tag="VQU28_001068"
     CDS             complement(15769..16638)
                     /locus_tag="VQU28_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HET2476434.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16680..17408)
                     /locus_tag="VQU28_001069"
     CDS             complement(16680..17408)
                     /locus_tag="VQU28_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476435.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGFAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17416..17895)
                     /locus_tag="VQU28_001070"
     CDS             complement(17416..17895)
                     /locus_tag="VQU28_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2476436.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVDHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17906..18604)
                     /locus_tag="VQU28_001071"
     CDS             complement(17906..18604)
                     /locus_tag="VQU28_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HET2476437.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18597..18863)
                     /locus_tag="VQU28_001072"
     CDS             complement(18597..18863)
                     /locus_tag="VQU28_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HET2476438.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18929..19855)
                     /locus_tag="VQU28_001073"
     CDS             complement(18929..19855)
                     /locus_tag="VQU28_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HET2476439.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFNLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19930..20745)
                     /locus_tag="VQU28_001074"
     CDS             complement(19930..20745)
                     /locus_tag="VQU28_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476440.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            complement(20749..21069)
                     /locus_tag="VQU28_001075"
     CDS             complement(20749..21069)
                     /locus_tag="VQU28_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2476441.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLTKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21366..21446)
                     /locus_tag="VQU28_001076"
                     /pseudo
     CDS             complement(21366..21446)
                     /locus_tag="VQU28_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(21490..23487)
                     /gene="metG"
                     /locus_tag="VQU28_001077"
     CDS             complement(21490..23487)
                     /gene="metG"
                     /locus_tag="VQU28_001077"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HET2476442.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEYDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23611..24870)
                     /locus_tag="VQU28_001078"
     CDS             complement(23611..24870)
                     /locus_tag="VQU28_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476443.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKKSKIYGFGGLP
                     ITTNISLAANFNIDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESV
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24872..25573)
                     /locus_tag="VQU28_001079"
     CDS             complement(24872..25573)
                     /locus_tag="VQU28_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476444.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25557..26756)
                     /locus_tag="VQU28_001080"
     CDS             complement(25557..26756)
                     /locus_tag="VQU28_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HET2476445.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTAVKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILIPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            27042..28388
                     /gene="gor"
                     /locus_tag="VQU28_001081"
     CDS             27042..28388
                     /gene="gor"
                     /locus_tag="VQU28_001081"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HET2476446.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESIAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
BASE COUNT         8500 a         6262 c         5073 g         8595 t
ORIGIN      
        1 aagttacttc aaagtacaga tttgaagctg cagataagac tgacgaaatc actttaatac
       61 taaatgaaaa tcaaaaagca aactaagaag ctagcctcag gctgtacttg agtacggtaa
      121 ggcgacgctg acgtgatttg aagagatttt cgaagagtat aaaacactgt tttgaagtgg
      181 tggataaatc ggctgggtaa aaactcaatc tcaggagaaa atgaagtaaa tctccccaca
      241 actatagaaa aagatttgat tctgctcctc acacaataaa atctttttct tttattaaaa
      301 acgaatcgtt tcacgtgttt tctcatatga ggtaacaaag cgattggtta caccaggctc
      361 tgccacttcc aatgcttgag aaatcttgtc aaatgaagcg tgataatcaa attctttttc
      421 aaaaatatct aaagcttcgt taaatgcttc ttgaatgcgt tcatcaaatg agcgatagcg
      481 gttagaatat tgcaagagtt gctctgtcaa agttgcatat tgtacaatat tataagtttc
      541 cgtttctaaa gcttccatat cattcgttgc aatttcaaga actcgggtaa cagattcaat
      601 gttaatcatt ttttgttcta actcaaccat taaatcctcg gtattattgc ttgccgtaaa
      661 gaataacttc aagaaagttt gtggaatacc tggcagattg cgtttttcca tgtatcgctt
      721 gatagtatgg agacgattga cataaacatt ggccttttga cgtgcattaa tatcatcttt
      781 ctcaatttgt gtcaggcgct cactaactga aatttgctca tcttcaatat cttttagttg
      841 agtttgtaaa tcctcaagat tttcttcaag aactgaataa gcttgggttg gttcttcttg
      901 atttgaagtt acctcaacaa tagctgcctc aaaactctct aattctgtct gaatacgacg
      961 aacatggctt gcagctgtct caggaagtaa ataggtcttg ttcaaacgtg caatatcttc
     1021 tcccaataaa gtattattct ctttcatatg ttgaaggtaa gttggaagag ttgcaagtag
     1081 attttccact actttctgag cagcaatttc tcgagtaaaa atatcataca aggcattgat
     1141 ttcctcttgt gcctgtccat tctcatattc ggcattatcc aattccaact gacgaatatt
     1201 ctcttggttt ttcttgaatg cttcataaag caagtggaaa cgcgcttcaa tatccgtttc
     1261 aacaaaatga taattagcat caattagttt acgataaccg gcttccaaat cctgtaattg
     1321 atctggcaat tctgtagaaa gcgtcgtaac caaggctgga acacgatcca caatatgact
     1381 taaggccaaa atgtgatttt ctgtattatc caaaatcact gcggcttcca cagggtcacc
     1441 cgatgaattc aaggttacaa attgtgaaaa ttcagattgg atattttcta attgtttttc
     1501 aatttcatcc aaggcttgac catactgttc tgaattttca gcaactctat gctgaagttc
     1561 ctcaaataaa tccaaagcat gaagaacacg accactattt ttagattctt gcttctctaa
     1621 gtctgccaaa gcattgcgaa ttgccgcaat atcttcttca atcaaagtaa tttgactctc
     1681 aatttggtca atttgatgac tggccttgag aaaacgaaat gaatggttat aaccttctgc
     1741 ttcaaagaga ttattttcaa tatcggcaaa agagttgaga gataaatcga cccatttttg
     1801 attccattca cggaaagcca cttgactttg tccaatcaag tgcatatttt ttacagcttc
     1861 tacttcatca tttactggaa gattgtatag ttcttctttt ctttcttcta gcgcctctaa
     1921 tctcccctcg tttcgcttac gtagaaagat tgccactaca taagccagaa ctaaaatgac
     1981 tgcaattgca accattaaat aaattagttg tccattagac atatcaaact ccttttttac
     2041 ttgaaacaat cgtaacgatt atatcatatt ttttagacca agggaactac ttctccctat
     2101 tttttagaca tcaagtgtac tatagacagc attttcttcg ataaactcac gacgaggctc
     2161 tactcgatcc cccatcaaca tatcaaagat tttatctgct tctgcagcat catctacaga
     2221 aactctagcc atcaagcgat gttcgggatc catggttgtt tcccacagct gatgatcgtc
     2281 catttcacct agccccttat aacgctgaat agtcggtttg gtacgacctt cactataacg
     2341 ggctaaagct tcttggagtt tgatttcttg atctgcaccc ggctggatat attctttaat
     2401 ctcgcttcca accttgacac catagattgg tggttgggca atataaacat aaccagcttc
     2461 taggattggt ttcatataac gataaatcaa ggttaaaaga agggtacgaa tatgggctcc
     2521 atcgacatcg gcatcggtca tcaaaacgag tttttggtaa cgggctttcg aaacatcaaa
     2581 ttctgcgcca aatcctgttc ccatggctgt gaaaagacta cgaatttctt cgttagctag
     2641 aatcttatcc atacttgctt tttcaacgtt caaaatctta ccgcgaattg gaaggatagc
     2701 ctgaaactca cggttacgac cagatttggc tgatccacca gctgagtctc cttcgacgat
     2761 gaagagttct gtttcagcag ggttattaga agaacagtct gctagtttcc ctggaaggtt
     2821 ggaaatttcc aaaccagatt ttttacgtgt gacttcacgc gcacgcttgg cagccacacg
     2881 agccttggca gctaaaatcc ctttttccac gatacgtttg gcaatctgtg gattttccat
     2941 gaggaaatcg gagaaggctt cactgaagag gcgattggta atcttgacca cttcgctatt
     3001 tcccaatttg gtcttggttt gtccttcaaa ctgtggattt gggtgtttaa ctgagataac
     3061 tgcagttaat ccttcgcgaa catcttcccc tgttaaatta tcttcattgt ctttcagtaa
     3121 cttattctta cgagcataat cattgataac acgagtcagt gccgtacgga aaccttgttc
     3181 atgcgttcca ccttcatggg tatgaatatt attggcgaaa ctcatgacat tttcatggta
     3241 acctgttgtg tactgcatgg ctacctcaac tgtgatatca tccatctcac cgtctgtata
     3301 gattggtgta tcaaagatta catccttgtt ctcgttgata tattcaacgt aactagcaat
     3361 cccaccttca taatgataat gcttggtttg ttccaaacct tggcgcttat cagtgataga
     3421 aatttgaaga ccgcgattta gaaaggccaa ctcttgaatc cgtttattta atttatcaaa
     3481 atcaaaggtt gttgtttcag taaagatttt tgggtccggt atgaagtgaa ccgttgttcc
     3541 agttctatct gtatccccaa ccacctcaag gtctgcaaca acatgaccac gacggtattc
     3601 ttggtaatga atcttaccgt ttttatggac atgaacgtct aattgagtgg aaagggcatt
     3661 aactactgac gaccccaccc cgtgaagacc acctgaaacc ttgtatccac caccgccgaa
     3721 ctttcctcca gcatgaagga ctgtaaagac ggtctcaaca gcaggacggc ctgttttttc
     3781 ctgaatatcg actgggatac cacgcccatc atccacaaca gtaatcgaat catctggctc
     3841 aataaaaact tgaatatggc tggcaaatcc tgccaaggcc tcgtcaattg agttatcaac
     3901 aatttcccag actagatggt gaagaccttc ttttgaggtt gatccaatgt acatccctgg
     3961 acgcatacga acagcctcta agccctctaa aacttgaatt tgactggcat cataatcctg
     4021 tgcctgcaga tttttgattt cttctgtcat cttattcctt tttcttatat gtctaatact
     4081 tgtcttaaat tcacgatact ggtaaacaag tgggtatcaa gtcctgcagc ttgacctgct
     4141 tcgatatcaa tcggccgatc accaatgaca agaccagagc taatctgata cttttctctt
     4201 aaataaagca tggattcggg atttggcttt ctcttaaagc ctgagctaga agtcaccact
     4261 tctgtaaaat aagctgctat agaggttttt tctaaaattt ccaaaacctg atcatttcga
     4321 tgagagacca aaaaatgacg gccaccttga tttaaaatgt cttccaatag gtcagaaact
     4381 ccttcaaata aaatcgggtg ttcaagctct ctggcttcat tttccttgta cttttctaaa
     4441 aaattctcta aattgggagc gaatgtctca atcgcaaaag gagtagaaac ctttaaagct
     4501 tgatagacac tgtcatggtc ttgtgtgata ccatacagtg ccaatgtttc aacaaatgca
     4561 gctgttgaag tttcataatt atccagtaaa gttccaccta aatcccagat gtaatcgtga
     4621 tatttcatac ccttcattat agcatttttt tatgaaaaaa gcgatgattt ccctgagttt
     4681 tccacataaa aaacgagaga ataactcccg ttttactgat ttttaccaaa gacatctcga
     4741 tagagcattc ctgttcgtaa actctctgcg tcccctccta gttgctccac caactcgtag
     4801 gcaaaggcaa gggctgttga aggaccccga ctggttgtca actgaccatc taccactact
     4861 gtttccttga cgtagtgacc atcaaggatt tgctcttgaa cgccgtcata acaagtgtat
     4921 cgcttatttt tcaatatctc tgcttgattg agggcaattg gtgccgcaca aatggctgct
     4981 agtttcttcc cttcttgctc gaagctttgc aattcttgaa tcaaggtctg attatcacgt
     5041 aaatgtgcag aaccaggcat acctccagga agaacaatca tatcatagtc tgataaatct
     5101 ccatcaaaga catgatctgc tcttacttgg attgcatgcg aacccgttac ttgctcttca
     5161 aaaccaacca tatcacatgt gatattggct cgacgcaaga catctacaac tgtcaaggct
     5221 tcaatttctt caaagccctg agctaacata actgctactt ttaccatgat tttctcctta
     5281 tttaatttgt tttaatacaa cctttttccg tttgaatggg acttttccgc tcttttcttc
     5341 agctagatta gtctggtaac tcatcgataa aagcaaacca acaccaatca gattactgat
     5401 aatagctgat cccccttgcg aaatgaaagg caagggaatc cccgtcaaag gaagtagtcc
     5461 agtcacagca ccgatattct caaagatgtg gaagagcaac atcataatca aacctgtgga
     5521 aatataagtg tagaactggt tatttgattt aagagtaatc ttcaacatac ggtaaatcaa
     5581 catgagatag agggcaataa ccaggacaga gccaataaag ccaaaatctt ctgcaataac
     5641 cgtaaaaatc atatctgact ctcgaactgg gataagcaga ttcgaagcat taaatccctg
     5701 accaaataag ccaccactcc caatggcaat ctgcccttga gcctgctggt aagtcgttgt
     5761 ttgggcaaac tcaaagggat tgagccaagc caaaatccga ttgatttggt aggtcggcat
     5821 tccaatctgg tgaagaaaag ctcgtccgtc cttgctaata aagatagcta agaaaccagc
     5881 aactcctgtt acagcagtca caaatactgg gataataatt ttccaagaaa cccctgataa
     5941 taaaacgatt cctgagaaaa tggctacaaa aaccaaagcc gtccccaagt cactttgaag
     6001 tgctaaaaga actaggactg gaatggtaaa gagaatcatc cagaaaatta acaaaaagtc
     6061 cagcgaaacc gtgcgtctcc attccttatg tttctttgta aattggacaa tgacacgagc
     6121 caacatgagg atataggata tcttcataaa ttctgacggt tgaaataggg taattccatt
     6181 tattgatacc cagtttttgg cacccgttga tgcaactaag cttggattat aaaatacaat
     6241 cggcaagatc ataagtccca agcctaaaat atatagaaag ggggtcacct tccaaagaaa
     6301 ttctgtatta aagagcatga ccacaaaacc aatcacaagc cccaaggcaa tccaggcgac
     6361 ctgctgccct aaaatgggca gaatattatt gggataatca tgactaacgg ctatatagat
     6421 agccaccaca ccgatgacca gtagaaaaaa tactggcaag agcaaactat aatcgactct
     6481 agagtcgaga gaacgtttca tatatactaa ccttatactt tcatacaata ctatttatca
     6541 aagttcatta aaaaatctat caatagcctc gtcaacttcg gatcgagaga tggttttaac
     6601 agtcgcttct tctgctagag atgctactat ttgtttgccg tatcgttttc cgatgattct
     6661 cctatccaaa ataagagtta aggaacgttg gtattcacgt ctcatacttc ttcccaaagc
     6721 ctgttttaaa cgaataatgg ccattggcaa ttgataatca taaaaggcat ttttcccttc
     6781 ttgattcagt tcttgattaa tctttttcgt caagggttct tgaggatttt ggaaaggaag
     6841 cctcggtaca acttgaatca caaaaggatg gcttgaaaaa tcaactccct cccagaaact
     6901 tgctgcacca agcaagattt gttgttcacc tttttcaaag cgtttcttta gctgatgaac
     6961 atccccattt ttatactggg ccaagtggct aactgtaagt aaatccgata ctgctagaag
     7021 catgtcttta gcggtaaaga gaaccaaaat cggttgctgg aaagcttgaa tttccactag
     7081 taaagcagct acctctctgg catagacttc taaggaggtt tctgttacca gagggaaatc
     7141 tttgaccata accacttctt gttcctgttt tccccgagat tcaatcttga caaatttggc
     7201 ttcaggatag cctaaaaggt ctgccaaaga aaccctctga ctaatctcaa gagtagccga
     7261 tactcccatg gcttggcaac tctcaggaag taaagaggac agaagagtac ggcctgattt
     7321 tgtagaagaa atatgaattt tcttttgact cgtttcagtt acttcaagcc agtaatcacc
     7381 ttcagctgta aaatagcgaa ccagctcatc aaagccttct acttctaatt ctgaaaaata
     7441 ctgatggaga ttgtccagag aatctaagat atttttccta gatttaccag actgaaattg
     7501 ttctatcaag tagagacact caaagcgaat actttctagt atccgttgtt gaaccctgtt
     7561 ttcttctcct actaaagcct tatcaattaa atcgataata gattgtatat cgtaggtctc
     7621 ttgaagcaga ttttctagag ctaacaaaat cttttggact tcatcaataa tcagtaaacg
     7681 gtcactgaca aattcaggat tatcttcaag tctggtcacg agataggcat gattagtcac
     7741 taaaagcttg caagtctctg ccctttcttg actacgtttc caaaaatctt ccgtcacaaa
     7801 taagctctgg gatgataaat tcccatcatg acgaaggtct gctagaaaat gttggtaacg
     7861 atagagttgc ccgatttcat ccaaatctcc tgtctctgtc tcagtaagcc agaccaagac
     7921 ttgcatttta aagcgtctaa ataagcgatt ttcatcattt tcctgcaagg aatgataaaa
     7981 ggcatccaac ttcagataat tttgtggtcc ctttaagcta tgaatatctg tatggaacac
     8041 ttccttgagg cgtttacctt cttcttccat gatttgattt tgaagaatct ttgtcggaac
     8101 actaagaaca atttgtcgct ctttggattg agataaagcg ggtaagagat agccataggt
     8161 tttcccaatc cctgtcggcg cttgaatcag agagacaggt tcatctttca atagcaagcc
     8221 aacctcttta gcaaaacttt cttgttcctc cctcacttca aggttcaaca gagaaatatt
     8281 tttagaaaag tcttgagata gttttcgtgg ctccagagaa gctgccgttt tcttaaaata
     8341 cagaccttga acttggacca agtctggaga actcaggata gattggttgc gataagtttc
     8401 ctcaataacc aggtaggact catataggag agcgtcagcc atttccagca agcgttccaa
     8461 gagaccttta ggaagctgag tcatcttttc ccgtaaaaaa agaagtaatt ctgctgtagc
     8521 ttgggcatct gaaagggctg tgtgtgcgtg tttaagagga attcctaatt ctcgacacaa
     8581 aatcggcaag ctatattttt ccagttcagg gaaaaagacc tgggccaatt cgaccgtatc
     8641 aacacgaggg tttcttagct catagccttc aaaaaataaa ttttccgcca agagattagc
     8701 atcaaactga acattatggg ctacaaaaat cccatcctcc accaagtcaa atatttttct
     8761 ggcaacttgc gaaaaatcag gtgcttgcgc cagacgttgg tctgtcaatc ctgtcagttc
     8821 tttgatatga gcatccaagg gttcatgtgg attgacatcc gtcgtatagt gatcgacgat
     8881 ttctccgtcc tcaatcacga caattcccac ttggataatt ttagccttac tacctgtgct
     8941 agttgcctct aaatccacca caacatagcg attaccaatc gttttcatta taaaaaatta
     9001 taccaaaaaa agggccattt ggcacctgca aacataggat aattttcaaa ctcaagaaca
     9061 ctacttttct taaaaatccc atcgaataga gcattctagc ctgctgattt tttaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgttttcatc ttccttctct caacgaatca
     9181 tgtattttat ttaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttggtcat ggctaaagtt
     9301 gccctcttga atcaagctac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcatc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgt tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tctttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaaa
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaagtc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgcgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagcaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagcg
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagcta ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaaat agtctacagt
    15361 gggtagacct tgcgaaattc ttctaaaaca accttgcttt caggtgtaaa agtcagccct
    15421 acttcaccca aacagtctgt gaaaaagtcc tcatgaacct ggcgccaata ggcaagtgat
    15481 ttgtctccct caccttcctt aaaggcatgt tgcgcagaaa cttgattgaa gagttcaaca
    15541 gaaacctttg taatttcgac aatgcagaca gactgatttt gactatctaa aatgacatcg
    15601 aaggtcccta cttggggaag tggttcgtct tctagtacat agaggtcata ggctgatgct
    15661 gttgctgtct tttctccttt aaacaccaaa tccgctaaaa ggtctggttc aactccaaaa
    15721 gcccaagcat cgatttcatc tccgatcaaa ggattgattt gcttgtattt attccacatt
    15781 tcttgcggta tcatgggtac tcctttgtaa ttttttactt tcttctttta tgtgtttaag
    15841 atgatctgga tggtcaatct ctaaatcaaa aatctctgga atagaactgt agtggataat
    15901 gcacttgata cccaactgat tcattttttg tatgaaagaa gtattcagat agcctgctac
    15961 agcaaaatta atcttgttct ttcttgcttt atcctgcata tctcttagca tatctaacat
    16021 tattggactt tccagatcat gccattgact gtttctcata gtcgcaaaaa caaaggaagt
    16081 caaatcattc attccaacta caatctttga aatgcccgtt tccagtatac tagataagtc
    16141 aaaatacgct gacggtaatt caatcatcgt tccgactttc ccagtaaagc catactgacg
    16201 caatactgta atagcttgtt ttaattggtc ggcatcattg acaaaaggaa agataacaga
    16261 tagattgggg ttggtttgat aaacttctgt aacgacatgt gcttcagcct gaaattcatc
    16321 caaacacgcc agtaaacgcc tggttcctct atagccaaac aagggatgcc cttcgtcaaa
    16381 aaactcttta gtccccacta aacaattggc ttctgtattc gttaattcag taaaacgata
    16441 ccaaacttct tcatctgagt acagggagca gatagtctct agataatctt ttacaaattg
    16501 ctgacaactt gctaatatga tgttttgatt aagctctctc aacaaatatt ccccacgaat
    16561 catgccgacg tggtgaaata gttgaggata aattttttca agaatttttt cgccactaag
    16621 ggcaagttga tttctcatag ccattcacct cgtcttaaaa gatacaatat catatataat
    16681 tatatcaaaa tttcctctag ttcaaaagct tcataaggtt cagataactc atatcctaaa
    16741 aattctgcta cttttttgga agcctcatta tgagcatccc aaagtggaaa catatctcta
    16801 tttaaactct ctagaatcat tgcagcacca agctttttag caaatccatt tccttgttcg
    16861 tttggtctag ttgcaacttc cacttcaact gctttacggt aaactaaccc tgaggaaatc
    16921 ccagcaatca gttcattatt tttaagaatc acaaagccaa atccaccttt taaaacaaaa
    16981 tcctgatagg actcaaaatc cccctgtaaa tcctgtgacc attcttccgt agaaaagcag
    17041 ttataaatat gattatcaat aggcacaata ttgtaacctt cctctaaatg agtaactaga
    17101 ttatttaaaa attcaacttg aaaatttgcc ttatctttaa aagaatagcg agtaaaagaa
    17161 tttagcccca cttttcgatg cagaaaatct tgccaattag tattttctga aataataatc
    17221 ttataatcta aaccatattt tctaacaaaa tcttcccaaa atctggaatc caactcccct
    17281 gctaggtata gaaaattccc aacatcataa aagcttgttg tcctatcatt gtttcggtag
    17341 acagttccaa tttgagattt aagaccatag agaaccatat ttttcttcca tgttttaaat
    17401 agttccatta tccctctact tcaccttcca attcatgtaa gaagtcttgt ccagttctgg
    17461 aaatcctaat aattcagact taaccttcaa gactaatggc gatgcatttt cttctgtaat
    17521 ctcttgaata tccatccaaa tatatccaag tgaatcattc gcaccatcag acacagcttc
    17581 cgaagtcgta acttgaggtg cactctcatt catttcaaca tcatacaagg ccatgacatg
    17641 gtcaaccata aaatttgtta actcttctct gacgaaaaca tcgtagattc gaggattaga
    17701 gtagcttcta acagtaaatc ccgtctcttc cataacttct ctagtcagcg tttccgtcag
    17761 tccttcacca agttgctgac tgcctccagg tagatcatac cgatgttgat aagggcctct
    17821 cgttttttca atgcagagta acttcccatt ttctaagcaa acagcgtaga ctccaaagtg
    17881 ttttttgatt tccatccaac tcctcctact tcaaagacca gccaccatct attgtcaaga
    17941 tttgtccttg catggcgctc gcttttccac ttgctaaaaa aagactaagc tctgctattt
    18001 cctctggctc aatccagcgc ttgattggag tttcactagc cacccagtca gccaaaccac
    18061 ctggttcaaa atccgcagcg gtcatagctg tcttgactgc tcctggagcg ataccaaaga
    18121 cctgaatccc agcttcagca tagtctagag ccaactgctt ggtgaagcca gccaaggcat
    18181 gcttggatga agtataggcg tgaccacctc cacctgctag gctagaagca atggaacaca
    18241 tattgatgat gattcccttt ttattttcca gcatttgtgt caaataatac cgagtcaact
    18301 ctactggaat aatgtagttg atttcaaaaa tctcttgaat gtcctgcgcc gtttgttcca
    18361 acagtggttt gtaatcatcc aaaactccag cagtattaca caaaacatcc acctgagggc
    18421 accagtcaaa aataggttcc aagtccaagg tcaaatctct ctgtaaaaag tgaaaatcac
    18481 cctctaagag tggcttttca ccttggtcaa ctccataaac ttgatagccc ttctctaaaa
    18541 agaggcgagc ttgagccaat ccgatccctg aactcactcc tgtaatgagt acacgtttag
    18601 tcatgcactt ctacccaatc cgttgccaaa acatcacaaa ctgtcgggct ccacatggaa
    18661 aaaccttctc cttcgccaga aacgttgatt aggaaataag gtgtcatttc aagtgcaagc
    18721 ccattttgct cgatggtatc aaagagttgg acatagtttt ccgcacctcc ccaaccagtt
    18781 cgtacatatt ttctcttagc ctttaaccca ggcaggatct cttcaaatgt catgtttttc
    18841 tcctttaatt ctacattctt catttaatta tagcaaaaaa ccgctttata cggctttttg
    18901 aatgtgagtt attcaaacct gctactactt acggcaaatt attccctgca gcaagataaa
    18961 tttcatacca ttcttttctt gttaagctaa agtttgccgc tcggctaact tctctcaagt
    19021 gcttaggatt tgttgtacct acgactgcct gcatttttgc tggataacgc aatatccaag
    19081 aaatggcaat agttgaagag gttactccat atttaatagc taaacgatca agtacttgat
    19141 ttaaagcttg aaatttctca tttccaacaa aattcccttt aaaatacccg aattgtaaga
    19201 cagaccatgc ttgaatgacc acatcgtgta attggcaata ttcaaaaatg ctgccatctc
    19261 gcatagctgc ttgactatct tccatattaa catgaaaagc tgattcaaat cctggagtaa
    19321 aagccgcact caattgtagc tgattaacag ctaacggctg cttgacatct tttttaagca
    19381 actccatcat cataggattt tgattagaaa ctccaaaatc tcgaacttta ccttgtttat
    19441 aaaggagatt aaaggcttct gctacttggt cagattccat caaagcatct ggtcgatgaa
    19501 ggagcaagct atctagatga tcaatcttca atctttgcaa aataccgtct actgatttta
    19561 taatatagtc cttagaaaaa tcaaaatagg taaattcttc aatgcgaatg ccacatttgg
    19621 actgaatcca catcttttct cttaaatctg gacgattttt taggacaaga cctaacagtt
    19681 cttcacaacg accacgacca taaatatcag ccaagtcgaa ggcattgatt ccaacagaaa
    19741 gtgctgtttc tacaagctct tcaacttctt ttacagactt atcttttatt ctcatcattc
    19801 cgagaacaat ttctgataat tctttgtcat cttgaccaag agttatgtat ctcatcaaat
    19861 ttttctcctt taatttctaa cattcttccc ttcattataa caaaaaaccg ctttgcaacg
    19921 gctttttgac tatacttcac tccattttat cttcttaaac ccacggaaca agacaaagat
    19981 tccaataaag aggacagcta aaggaataac ttttgtaagg aaaacatttg aaattcccat
    20041 ccactcataa taacggagca gagaacccac cacaagatgg gcaataatca tactgacaaa
    20101 tggacgaaag accgcttctt tccaattcca aataccgata actagcgaaa tcgtaaagac
    20161 agacaaacta tcccagggag ccggaatata aaaggctcct tcttgtatga agcttgccat
    20221 tcctacatat cctaaaacaa ctagaagaac tatagtccca acaacaatgt aagtgccaat
    20281 tttcatttta ggagaatctt ggactaaact tcttcgtaaa attgtggcca caagtccaaa
    20341 tccaatcaga aaaataagaa gttgccctaa aaatgtgagc aaattgactg ttaagagagg
    20401 acctttagaa aaatcactta gtagttgata ataacgtaat acagccagga caagaattgg
    20461 cgtcaaaagg gactctttga tagaactgcg aggtgctccc ttgagaatct ctttcattat
    20521 ttttttagga ttcttaccta gataatcctc tgcactcatg ccatctcgtt ctgcttctga
    20581 gaaatctagc atcatcaaat agatctgctc tctgagatag tcttcatcat agagaaatcc
    20641 agcaagatta aaactttccc acaactcctc aaaatacttt tgattctcct cagaaaactc
    20701 atgtagcaaa gcgcttgttt cttcgtaata cttcattttc ttcatggttt aacccccatt
    20761 cttaatccct tctacttttt gactcaaatc gtcccattgc tgccaaaaga ctgagacacg
    20821 ctcttctcct tctttcgtta atgaaaaata cttccgatct ggaccatctg gcgacgggcg
    20881 catgtcgcct cttatccatt gatttttttc taacttttgc aacaaaggat aaatagttcc
    20941 tggaacgata gtatcaaatc cagcctctcg caaagtctga accaactcat aaccataccg
    21001 ctctttttga ccaatcatat ccaagacaca accttcaaga acacctttta atagctgagt
    21061 ttctttcatc acttctccct tctaatctat tttgtaatac ctactagtaa cttcacctat
    21121 agtatattac ttctacacta gtttgtaaag cataatagtt aatactcttc gaaaatctct
    21181 tcaaaccacg tcagcgtcgc cctaccgtat gtatggttac tgacttcgtc agtttcatct
    21241 acaacctcaa aacagtgttt tgagctgact tcgtcagttt catctacaac ctcaaaacag
    21301 tgttttgagc tgacttcgtc agtttcatct acaacctcaa aaacatgttt tgagctgact
    21361 tcgtcagttc tatctacaac ctcaaaacag tgttttgagc aacctgcggc tagcttccta
    21421 gtttgctctt tgattttcat tgagtataaa taaaaaaaca gaactagtct gaactagtcc
    21481 tgtctacttt tacccaatca cacttccatt tggtacagct ggatcaactg tgagaagggt
    21541 taatttgcca tcatattcag ctgatagaat cataccctgg ctgacatatt ttttcatcat
    21601 tttacgtggt ttgaggttag caacgatttg aactttcttg ccgaccaatt cttgttcatt
    21661 tggatagtat tttgcaattc ctgaaagaat ctgacgatct tctccatcac cagcatccaa
    21721 gcggaattga agcaacttat ctgaaccttc tactttagac acttctttga cttctgcgac
    21781 acggatttca accttgtcaa agtcttcaaa cttgatttca tccttgttta gtttgagctc
    21841 aacttcgtcc ggattccatt ctttttcgac tgctggttta ttgccttcca tttgttcctt
    21901 gatataggcg atttcttctt ccatatttag acgtggaaag ataggtgttc ctttggcaac
    21961 tacagtcaca tctgctggga agtcagccaa actcaagttt tcaagactag aaacttcttc
    22021 caaaccaagt tgagtcaaaa ctgcacgact agtttccatc ataaatggtt caatcaagtg
    22081 agcaactaca cgaatgctgg ctgccaagtg gctcatgaca cttgccaatt ggtcacgaag
    22141 agcttcatcc ttggccaaga cccatggtgc ggtctcatcg atgtatttat tggtacgaga
    22201 gatcagagtc cagactgctt caagcgcacg tggatagtca actgcttcca tgtgtgtatg
    22261 gaagtctgcg attgattgtt ctgcaacctc agcaagaaca tgatcaaatt cagtcacacc
    22321 ttctacatag gcagggattt gtccatcaaa gtacttatta atcatggaaa ccgtacggtt
    22381 aaggaggttc ccaaggtcat tagccaattc atagttgata cggccgacat agtcttcagg
    22441 agtaaaggtt ccgtctgaac caactggaag gttacgcatg aggtagtaac gaagtggatc
    22501 tagtccataa cgctctacca acatttcagg gtaaacgaca ttcccttttg acttagacat
    22561 ttttccgtct ttcatgacaa accaaccatg ggcaatcaaa cgatcaggta atttaacatc
    22621 caacatcata agaaggattg gccagtagat agagtggaag cgaaggatat cttttcctac
    22681 catatggaag actgttccat tccagaactt gtcaaagtta ccatgttcgt cttgagcgta
    22741 gccaagagct gtcgcatagt taagaagggc atcaatccaa acgtagacaa cgtgttttgg
    22801 atttgatggg acaggcactc cccatgtaaa ggttgtacga gataccgcca aatcttccaa
    22861 gcctggctcg atgaagttgc gtagcatttc attaaggcga ccatctggcg tgataaattc
    22921 aggatgagct ttgaaaaatt cgaccaaacg gtcttggtat ttgctaaggc gaaggaagta
    22981 tgattcttca gaaacccatt caacctcatg acctgatgga gcaataccac cagtcacatt
    23041 tccagcttca tcacggaaaa cttctgccag ctggctttct gtaaagaatt cttcgtctga
    23101 tactgaatac caaccagagt attcacccaa gtagatatca tcttgagcaa gtaagcgttc
    23161 aaagacctgt gcgacaactt tttcatggta gtcatcggtt gtacggataa atttatcgta
    23221 tgagatatct agtaattgcc agagttcttt aactccaacc gccattccat caacataggc
    23281 ttgaggtgta ataccagctt cttccgcttt ctgctggatt ttctgaccat gttcatcaag
    23341 acctgtcaga taaaatacat cgtagcccat caggcgtttg taacgtgcta ggacatcaca
    23401 tgcgatagtt gtgtaggcag aaccgatatg aagtttccca gatggatagt aaatcggcgt
    23461 tgtaatataa aaattttttt cagacataat ttttcctttc caggcaaatg aaacctgttt
    23521 ttctaacact tcattatatc acatttttaa tgaatttcga tagggaaatc catacaaaaa
    23581 caagatagac gagtgtccat cttgttgatc tcattcataa cgaagggctt caattggatc
    23641 aagtttcgat gccttgttgg ctggcaagac tccaaaaatc ataccaacac tagccgaaac
    23701 tgcaagacta aatagggcaa ctgggattga tactccaact tctatacctt ctattaaacc
    23761 ttgcagtaac aaacctgcta aggcagttaa accacttgca attgtcaagc caattaagcc
    23821 acctaacaag gtcaaaatca tggattcaat caaaaactga attaaaatat tggcacgtgt
    23881 tgcacccaaa gctttacgaa gaccaatctc acgagtgcgc tctgtcaccg aaaccagcat
    23941 gatgttcatg acaccagttc ctccaacaaa gagagaaatc cctgcgatgg aactaataat
    24001 cgtcgtcata aaactaaacg attgttgaat ttctgcaaat acaacggact catctgccac
    24061 ctggtattct ccctgttgta agcctgcaag ctctgtcatt tttcgtgcca gttctggacc
    24121 cagagttggg gttaaactgg tatcattcac tcgaaagaca atactagcta tttcatctat
    24181 attaaaattc gcagcaaggg agatattggt agtaataggc aagccaccaa acccatatat
    24241 ttttgacttt ttagcctccg gactagtata aaccccaatg acccggtaac taaatccatt
    24301 gacttctaca accttgttaa tagcctcttg aggagattca aataaactaa tggacaattc
    24361 ctcatctagc aaaataacac ttgcaaactc tttgaaatct tgctctctca gactacgacc
    24421 tgcaataatt tcattcttaa cagcgtccat gtaagttctg tttccacctg tcaaattagc
    24481 attctcaacc tttttatctt gataggtcaa gatggcattc gttgaattgg ttacatagta
    24541 actatccact cccttcagtt tagctgcctc ttgaacccag gattcttgcg gttttggcgg
    24601 ttcaacagga acttcctctt cctttccaga aaccgtaaaa gctgattgtt tctgagtaaa
    24661 agacccatct ttactttttt taggagagaa aaagacgcta atatttttct gagatttagt
    24721 catatcttta ttgacttgac gagataggga atcacccaaa gccataatca caacaactga
    24781 tgaaacaccg ataataatcc caatcatagt aagcaaagaa cgcatcttgt gagccatgat
    24841 agatgaaaag gcaaatttca gattctgcat cttagttttc ctcctttcct aactgagcac
    24901 tgtcagacga aatgacccca tcccgaatga caatctgacg tttggcatag gcagcaatct
    24961 caggctcatg cgttaccatg ataatggttt ttccttcttt attcaaatca accaataatt
    25021 gcataatttg gttacctgtt ttggtatcca aggctcctgt cggttcatcc gctaggataa
    25081 tagaaggatt gtttaccaag gcacgcgcaa tggctacacg ttgcttttga ccaccagata
    25141 attctgaagg taaatggtga ctacgttctg tcaattcaac cttgtctaaa tattcctcag
    25201 ccaacttgcg acgttttgaa gacgaaactc ctgcgtaaat caagggcaat tctacatttt
    25261 gcagagcatt gagcttcgat agaagaaaga actgctgaaa gacaaaaccg atttgttggt
    25321 tacggacctt agctagttgt ttttcaccaa gcccagccac ttcttgacct tcaagataat
    25381 attctccact ggttggtgta tccaacatgc caatcgtatt catcagagtg gacttaccag
    25441 acccagatgg tcccatgatg gctacaaatt caccctcatt cacttctaga ttgatatttt
    25501 tgagaacctg cagttcttgg tcaccattac ggtaacttct gaagatattt tttagactaa
    25561 ttagttgctt catcagcctt cacctctttt ccttcttcca aggaagatgt tggattactg
    25621 atgaccttag caccgttcgt taaaccagaa gtgatttctt gattttctgc gtcagcattt
    25681 cccaatgaaa cctcaacttt tttagccttt tgttgttcat ccacaatcca gacataattt
    25741 ttactatcat ccattactag actgctaaca ggaataagaa tagccttagt tttgctttta
    25801 acctcaatgt tgacagaaaa accttgtttc aaatcaccaa cctcgcctgt cacatcaata
    25861 gtataagggt atttagaacc tgtattattc ccggctgctg gactagctgc ttcaccattg
    25921 tttttaggat agtcagaaat atagcttaat ttcccagtcc attttttatc aggatacact
    25981 ttagaagtaa agcttacttc ttgacctaca gaaaggttgg ctagattgta ctcagacaat
    26041 tctcccttga cttgtaaatt ttcattgctg acaatatgaa ccataacttg actcgcccct
    26101 gttggagatt tagaaacatt gctattgact tcgactacag ttccctctag ggtactgaga
    26161 acagttgttg catccaattg actttgagcc ttgcttaatt gcgctgcagc atctgcacgc
    26221 gcatcacggg catcacccaa ttgagcatca atagaagcaa cagaatttcc agccactgga
    26281 gttgggcttt gcaccgttgc atcttctcct cctactggcg ctggtaactg tggagcctga
    26341 gctgaagcgg cttcatttcg tgcttgattg agttcattga tatgacgatc tgccttagct
    26401 actgctcgac tagctgaatc ataggccgcc tgcgcttctg aactactgta cttgactaaa
    26461 gcctgccctt cgctgacctt atcgcccaca gaaacaagga tttcatctaa atcaccctta
    26521 ctagcatcaa aataaacata ttgttcattt tttgctgtta ctgtccctga caataaaaca
    26581 gaggatgcca cgcttccttc cttggcaaca acaagatgag taggctcatc ttttacagca
    26641 gtctgagaag gttgtctaaa aagtaaaatc cccccggcac ccaatacaac tacactcgca
    26701 gcaccgattg ctgcatacag ttgccacttt ttagctttac cattcttttt cttcataatg
    26761 aaactccttt tcttttttac aatactttgc tattatacca aatttccctc cagcaaacaa
    26821 tgcagttcag atggaaataa tggcagttca ggattaaaca atcgttcgga attttgcttt
    26881 tctattgtac tagatatata atacactatc ttgacttatt tggcaagctt ttttgcaatt
    26941 ttttgtgcgt agcagatttt tgcaatcaaa tcaaaaaaag aataaaatat aactatcaaa
    27001 aaagacactc tactatttaa aagattacaa aggagttttc aatgagagaa tatgatatca
    27061 ttgctatcgg tggaggtagc ggaggaatcg ctaccatgaa ccgtgctggt gaacatggag
    27121 ccaaagcagc cgttattgag gaaaagaaat taggtggaac ctgtgtcaac gtcggttgtg
    27181 ttcctaaaaa aatcatgtgg tacggggcgc aaatcgctga gactttccat caatttggag
    27241 aagactacgg ctttaagact actgatctta actttgactt tgcaacccta cgtcgcaatc
    27301 gtgaagccta cattgatcgc gctcgttctt cttatgatgg tagttttaaa cgcaacggtg
    27361 tagacttgat tgaaggtcat gctgaatttg tagattctca tactgtaagc gtaaatggtg
    27421 aactgattcg tgctaaacat atcgtgattg ctacaggtgc ccatccaagt attcctaata
    27481 ttcctggtgc tgagctaggt ggctcttctg atgatgtatt tgcctgggaa gaacttccag
    27541 agtcaattgc cattctaggc gctggttata tcgccgttga attagctggc gtactccaca
    27601 cttttggtgt caagacagat ctctttgttc gccgcgatcg tcctttacgt ggttttgatt
    27661 cctacatcgt tgaaggtttg gtcaaggaaa tggaaagaac aaacttacca cttcacactc
    27721 acaaagtccc tgtcaagtta gaaaaaacta ctgacggcat taccattcat ttcgaagatg
    27781 gtactagtca cacagctagc caagttatct gggctacagg tcgccgtcca aacgttaagg
    27841 gcttgcaact tgaaaaagct ggagtgactc tgaacgaacg tggctttatc caagtggatg
    27901 aataccaaaa tactgttgtt gagggaatct atgctctagg tgatgtaacg ggcgagaaag
    27961 aactgactcc agttgcaatc aaggccggac gtaccctatc tgaacgtctc tttaacggaa
    28021 aaactactgc aaaaatggat tactcaacta ttccaactgt tgtcttttca caccctgcta
    28081 tcggaactgt tggtttgaca gaagagcaag ctattaaaga atacggtcaa gaccaaatca
    28141 aggtttataa atcaagcttt gcatctatgt actctgcttg cactcgcaac cgtcaagaat
    28201 cccgtttcaa actcataaca gctggttcag aagaaaaagt tgtcggactt catggaattg
    28261 gctacggcgt tgatgaaatg attcagggat ttgccgttgc tatcaaaatg ggagcaacca
    28321 aggctgactt tgatgcaact gtggcaattc acccaactgc atctgaagaa tttgtaacca
    28381 tgcgttaatc gaaacaaaag aagctgatat aaatatgtct gcttcttttt
//
LOCUS       DAVBCB010000026        27272 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVBCB010000026 DAVBCB010000000
VERSION     DAVBCB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.026"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            225..1907
                     /locus_tag="VQU28_001082"
     CDS             225..1907
                     /locus_tag="VQU28_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476447.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQTAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLLPLLDQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHRPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLANLDNMSIS
                     KGQVQLQNELVKETPELQSLFKLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKAISSFIQTEGRYLWEKQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEKEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHRKGHTIVMITHDMQLMLDYSDRVLVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            1892..2722
                     /locus_tag="VQU28_001083"
     CDS             1892..2722
                     /locus_tag="VQU28_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2476448.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATVFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVASLLLSLQNQGRFYNPWK
                     "
     gene            2915..3418
                     /locus_tag="VQU28_001084"
     CDS             2915..3418
                     /locus_tag="VQU28_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HET2476449.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     regulatory      3472..3706
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            3871..4434
                     /locus_tag="VQU28_001085"
     CDS             3871..4434
                     /locus_tag="VQU28_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HET2476450.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTIGLTVAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYILLKPTLKHYER"
     gene            4448..5074
                     /locus_tag="VQU28_001086"
     CDS             4448..5074
                     /locus_tag="VQU28_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2476451.1"
                     /translation="MKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLTLAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASLCVGLGALFIEFPFYDKLRFQWRFKGKQK"
     gene            5186..6058
                     /locus_tag="VQU28_001087"
     CDS             5186..6058
                     /locus_tag="VQU28_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000525616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476452.1"
                     /translation="MGMLLATRLKNKRKELKLSQKELAEGICEQGQISRMEQGKYSPG
                     SELLFQLSKRLKVSMDYFFEDTEVSSLENIDKFKELSKKFLDEREYESLHYIYELEKS
                     KRARLSAKDQIYLDWIDTLVQFCYLNHQTGAVAKLEKILLSLKKSDFYYLQLGNTLAN
                     FYFDMGQEDTYQELYQLLKENLQSLSVHHLEQLELMIKFRYNYCRYLWLNERTSEAIE
                     EITETIAICKRYNSSYRLADLYCLLGSISEGFAERDRILGFYQVSYQLYKHEENQRMM
                     LEIEKEMKLLESED"
     gene            6188..6325
                     /locus_tag="VQU28_001088"
     CDS             6188..6325
                     /locus_tag="VQU28_001088"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476453.1"
                     /translation="MKRSQVVKRCILIALLLLFLLNVLSGSLSVTTRGVDPRPAYGVS
                     L"
     gene            6749..7663
                     /locus_tag="VQU28_001089"
     CDS             6749..7663
                     /locus_tag="VQU28_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HET2476454.1"
                     /translation="MKLYQINDGIQIFESSNLEKIVIRNYRNGKKITIDHQPLLKKLL
                     LFLEVPKREEEILLYLPELSNKTLRSVLTNFMSLNIIHCQDTLIIHKKINILIIGLGT
                     TGGYLADGLCRSNINSKLFLLDKDRVDETNVGRQIYLRDDIGQYKVDVFKQKFSDKEI
                     KVFKIFINSKKDLEKICKREGIDLVIQCGDSPSPRELGKIVNTVCDTLQIPYIVNTGY
                     MSNVVPLPEFYYPNNRYNFNYKHQFSDEKLLFSQVLSKADYMVAIQSSFVMLMQIKNF
                     IFNEEPIYYGYRGYYNSVTLAWEVEKID"
     gene            7656..8669
                     /locus_tag="VQU28_001090"
     CDS             7656..8669
                     /locus_tag="VQU28_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476455.1"
                     /translation="MTKYLELDYSHSYILEGFRKNDAFIENHKECLDMLSDKIWRDNK
                     YMNTEKNISSPTRTILSRYTCNILSSLFIDISKNSIEKLIVTCENSDLNWIYNYYKSV
                     YNGVFCELRQLIQQYCKVKDKYEKWLFIKAIINQGIRQNEKEVVEFFLKQLKDNNQGI
                     FDYINSFSFYLLKFYSKDKSRIFLEEQLDIDDFLGSDKEDYARSLVFKNYASLLPDTS
                     ELKRNIMEKCLSQTPQDTDLWKEWFETYASQKEIRQKATEIFKHGYSDISLLKLVKIE
                     PDDTESLIRMIILCTSDLNSNIARYLISFLSDGRLKEFLELFVENFDFLNIIEGKTSE
                     INF"
     gene            8653..10245
                     /locus_tag="VQU28_001091"
     CDS             8653..10245
                     /locus_tag="VQU28_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476456.1"
                     /translation="MRLIFRIIRKDIILYSILTFLLSIAMVSEAFLMQFIVDSIKFGE
                     KKYVLISIFIVIFLILQTLLYYYQQFLTAVLSKKSAYEYRKLIFKNIQQTSLRLVTGE
                     KNDSILASLTTQIDQIEYNYFYSVYWGGYLLCQLVVAIVMSLYLNPLMSVLTICLSLP
                     NLFVALLFKNSLEQKQEELINETNSSIAVTQDLIEGITDWKIANREPNIFRVFESKTL
                     KLLEKQVKVEKFQYIVVSLNQLFSNVLYFGSWIVGGFLIIRGQLSLGSLIAFSQLLAR
                     ISYPVYASSDLLSKYISGKKIVEILSKEFIFSDESRNYPDEFNVISFEHYFTNIDEKD
                     MPFDKEFYKNKKYLLKGKSGSGKTTILKSILREYDDYSGIVKINGVDVRDIKESVLFD
                     NIAYVSQKPHLFSASLRDNLTLFSEDFTDNEIVEVLKFVELSKWANKDCLDMMLSNDD
                     IKLSGGEVKRVSLARALLMNKNILLLDEFSAGIDYETLLKIEKKLIDLDKMIIYVTHV
                     DIDRSGKQFDDVIDLDCLFISS"
     gene            10437..11340
                     /locus_tag="VQU28_001092"
                     /pseudo
     CDS             10437..11340
                     /locus_tag="VQU28_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091364.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11358..11495
                     /locus_tag="VQU28_001093"
                     /pseudo
     CDS             11358..11495
                     /locus_tag="VQU28_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
     gene            11523..11864
                     /locus_tag="VQU28_001094"
     CDS             11523..11864
                     /locus_tag="VQU28_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476457.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLKNHI
                     LPLWIGMVSFF"
     gene            11837..12148
                     /locus_tag="VQU28_001095"
                     /pseudo
     CDS             11837..12148
                     /locus_tag="VQU28_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            12276..12863
                     /locus_tag="VQU28_001096"
     CDS             12276..12863
                     /locus_tag="VQU28_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HET2476458.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            13253..14860
                     /locus_tag="VQU28_001097"
     CDS             13253..14860
                     /locus_tag="VQU28_001097"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HET2476459.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(15419..17050)
                     /locus_tag="VQU28_001098"
     CDS             complement(15419..17050)
                     /locus_tag="VQU28_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HET2476460.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGITITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            17343..22322
                     /locus_tag="VQU28_001099"
     CDS             17343..22322
                     /locus_tag="VQU28_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HET2476461.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAAEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAVLASLGLASLALTLRRKREDKD"
     gene            22412..23608
                     /locus_tag="VQU28_001100"
     CDS             22412..23608
                     /locus_tag="VQU28_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HET2476462.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            23744..24271
                     /locus_tag="VQU28_001101"
     CDS             23744..24271
                     /locus_tag="VQU28_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HET2476463.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            24348..24704
                     /gene="glnR"
                     /locus_tag="VQU28_001102"
     CDS             24348..24704
                     /gene="glnR"
                     /locus_tag="VQU28_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HET2476464.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            24741..26087
                     /gene="glnA"
                     /locus_tag="VQU28_001103"
     CDS             24741..26087
                     /gene="glnA"
                     /locus_tag="VQU28_001103"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HET2476465.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(26183..26302)
                     /locus_tag="VQU28_001104"
     CDS             complement(26183..26302)
                     /locus_tag="VQU28_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HET2476466.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            26790..27272
                     /locus_tag="VQU28_001105"
                     /pseudo
     CDS             26790..27272
                     /locus_tag="VQU28_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         8592 a         4568 c         5792 g         8320 t
ORIGIN      
        1 catacctacg gcaaggcgaa actgacgtgg tttgaagaga ttttcgaaga gtataaaatg
       61 atggggaagg ctgggcaact agtctttttc gatgtttata gattagttag gcaagtaagc
      121 taggatagga atctttttgg tgctaagatt ttaagagaag agactgcaag aagggcttga
      181 tttatgataa aatagaagtc taagaagcga atgaagagag taagatgaaa gaagctataa
      241 ttgagtggaa ggatttctct ttccggtatg aaacacaaca agaaccgacc ttgcaaggga
      301 tagacttgac catttacaag ggagagaaag tcttaattgt tggaccatct ggatcaggta
      361 aatctacctt gggtcagtgt ttgaatggga ttattcccaa tatttacaag ggtcagacat
      421 atggagaatt tttgataaag ggtcaaacag cctttgatat gagcatctat gataagtctc
      481 atctggttag cacagttttg caggatacag atgggcagtt tattggcttg tctgtggcag
      541 aagatttggc gtttgctctg gaaaatgatg tgacagccct agatgagatg aaaggtcgtg
      601 tttataaatg ggctgaaaag ctggaccttc ttcctttact ggatcagcgt cctcaggatt
      661 tgtcaggtgg acaaaagcag cgagtcagtc tggctggtgt cttgattgat gaaagtccga
      721 ttctcttgtt tgatgagcca ctcgccaatc tagatcccaa gtcaggtcag gatattatcg
      781 aattgattga ccagattcat aaggaagagg ggacgacgac tcttattatc gagcaccgtt
      841 tggaggatgt tctgcatcgc cctgtggatc ggattgtctt gataaacgat ggtcgtatcc
      901 tttttaatgg gagccctgac cagttgcttg cgactgattt attgactcaa aatggaattc
      961 gagaacccct ttatctaacc actctccgtc aattaggtgt ggacttagtc aaggaagaac
     1021 agttagcgaa tctggataac atgtctatct caaaaggtca ggttcagctg cagaatgaac
     1081 tggtaaaaga aactccagaa ttgcagtcac tctttaaatt agaggacgtg tctttctctt
     1141 atgatgatag accgatttta aaatccctac atttagatat taaaaagggt gaaaagattg
     1201 ctattgtcgg aaaaaatgga gcagggaaat caactctagc caaggctata agtagcttta
     1261 ttcagacgga aggacgctat ctttgggaaa aacaggatat aaaaggcgat tctgttgcag
     1321 agcgggcgga acgagtaggc tatgtgctac aaaatcctaa tcaaatgatt tcaaccaata
     1381 tgatttttga tgaggtggct ctagggctcc gtttgcgagg tgtggatgag aaggaaattg
     1441 aaacgagagt ctatgaaacc ttgaaaatct gtggacttta tgaattccgt aattggccta
     1501 tttctgccct gtcatttggt cagaaaaaac gtgtcaccat tgcttcaatt ttggtcttag
     1561 gagctgaaat tattctccta gatgaaccga ctgcaggtca agatcagaag aactatactg
     1621 agattatgga atttctcgaa gagttacatc gaaaagggca taccattgtc atgattaccc
     1681 atgatatgca attgatgctg gattattcag accgagtcct tgtcatggtg gatggagaat
     1741 tgattgccga tactgttcca gccagtctgt tgagcgatcc tgagctgtta gtaaaagcca
     1801 atctaaaaga aacctccatc tttaacttgg ctaagaaact agatgtggat ccactggatt
     1861 taacggcatt ttacaaagaa aggagagagg gatgcaagct aaattaatcg gttaccagca
     1921 tagagatact gtgattcatc gcttgtcagg agctgggaaa cttctctttt tcattctggt
     1981 atcattggcg gccatgatta gctatgatac cagaatgctc ttgctgattg ccatcttttc
     2041 agtctttctc ctctatttat cagaaattcg ctttaaagat gtttcctttg tagccgtttt
     2101 tgcgacggta tttgccgttt taaacgtttt gatggtctat ctcttttctc ccgagtatgg
     2161 ggttggactt tatggagaga gaagtgtgat ttggcaggga atcggtgtct acactctaac
     2221 cagccaggag ctcttttatc tgctaaattt ggccattaag tatctttgca ccattcctct
     2281 ggctattatc tttttgatga caacccatcc tagtcagttt gcttccagtt taaatcaaat
     2341 tggtgtgccc tataagattg cttattctgt cagcctgacc ttgcgctata ttccagattt
     2401 gcaggaagaa ttctttacta tcaagatgtc tcaggaggcg cgtgggatgg aattatccaa
     2461 gaaagcttct cttatgcaac gaatcaaagg caatctgctc attattacgc ccttgatttt
     2521 tagctcgcta gaacgcattg ataccattgc gactgccatg gagcttcgcc gctttgggaa
     2581 agaaaaaaaa cgcacatggt atagttatca ggccttgaaa aaaggagact atattacctt
     2641 gctcttggca accttgtttt tagtagctag tttactactt agcttgcaga atcagggacg
     2701 attttacaac ccttggaaat agcttgaaaa aattgaaaaa atcaagtcgt ttctattgac
     2761 aatgattctg aaagtgttat actaagaaag tagtttcgct gatttacttc aaacctgttg
     2821 tgaggtaagt taacgatgcc ttaaccacgc tgtttgctga gcttgactcc gggcagtgtg
     2881 gctatttttt tgcaatggtg aaaggaagca agtcatgaca aatcacattg tattatttga
     2941 acctcaaatt ccacaaaata cgggcaatat cgcgcgtact tgcgctgcga ccaattctcc
     3001 cctccacatc atcaagccta tgggctttcc tattgatgac cgcaagatga agcgggctgg
     3061 attggattac tgggataagc tagagattta tttttacgag agtttggaag atttcatatc
     3121 tcagatgaag ggaaaactct atctgatttc gaaattcgcg gaaaaagtct attctgaggt
     3181 ggatttatcg actgacgagg accattattt tctctttgga cgtgaagaca agggcttacc
     3241 tgaagatttt atgcgagaac atcctgagaa ggctctccgt attcccatga atgatgaaca
     3301 tgtccgcagt ctcaatgtgt ctaataccgt ctgcatgatt gtctatgagg ctcttcgcca
     3361 gcagaacttt gcaggtcttg aactcgtcca tacctatgaa gtggataaat tgaaataaaa
     3421 aatgaaaatg aacaaaatgc ttgcgcttgc aagcgttttt tgttatgata aaagagtctt
     3481 cagggcaggg tgtgattccc gaccggcggt gactttaact aggaaatgat cttttccttt
     3541 tatactttgt tgacaagctt tgcctaacca gaagttatgc ctacagcttg tcgcctagtc
     3601 taaaagaaaa atctctattt ccttctcttt aaagaagtcc gcgagcgcaa gctgatgtgg
     3661 tgagattcca caaccgacag tatagtctgg atgggagaag acgaaagaat agctttgtct
     3721 gttttgatag atttatagcc ggattgtaac cacttgcttt gtttttcttt aatagagtag
     3781 gagggaactt ttgggatata aaaagtgaga atagatagag gaatcctttc caacttcttc
     3841 tgattttata gaaaattgga ggaacctgtt atgacaaaca cacgtcgact ttcgaccatt
     3901 gcaattttat cagccatctc atttgtgctg atgtactttg actttccgct tttaccagcg
     3961 gcatctttcc tcaagatcga atttagtatc ttgccagtcc ttgtgggtct ggtggtcatg
     4021 gatttgcctg ctgctctagg agtgctcttg cttcgctcac tcttgaaatt gcttcttaac
     4081 agccaaggag tgaatactta cattggtttg ccgatgaata tcgtagcttt gggagttttt
     4141 gtcatcgtat ttgctttgat ttggaaaaag gaacggacaa cccttcgttt cctactaggc
     4201 tctctagctg ggactattgg tttaaccgtg gctatgttag ttctcaacta tgtttatgct
     4261 gttcctttgt acgctaagtt tgctaacttt gatattggaa aaattttggg actttccaac
     4321 tacctaatga ccatggtatt accttttaac ttgattgaag gtgtaatctt ttccgtttca
     4381 ttctggttgt tgtacattct cttgaaacca accttaaaac attatgaaag ataaacaaac
     4441 atttttaatg aagggcagtt ttgccctttt acttttcgtt attcttggct atatggtcaa
     4501 attttaccct gaaatgttgg tcaattttga ccaatcgatt cagactgcca ttcgaggaga
     4561 cttaccagat tatttgacta ttctttttcg atccctcaca cgtctgattg atattccagt
     4621 gattatcact tgggttgtca ttatagcttt tgtcttttat cgtaagcgat ggaagataga
     4681 aagtttcttc atgctgggaa atctgacttt ggcaggtctt ttaatcgtga cctttaaaaa
     4741 tatctaccag cgcccacgac cagctatctt acatctggtg gaggagaagg gattttcctt
     4801 cccaagtggg cattctctgg ctgtaacgct gatggtaggg actttgattg ttattcttag
     4861 tcagcggatt aaagatcagg tctggagaaa aatcgtacaa atcgtccttg gcctctacct
     4921 agtcagtgtg ctggtatcaa gagtctatct gggagttcat tacccatcag atgttcttgc
     4981 cagtctctgt gtgggcttgg gagccctgtt tatcgagttt cccttttatg ataagctccg
     5041 cttccaatgg cgatttaaag gcaaacagaa atgatggaaa agataaaaaa ttgtacaaat
     5101 acaaatagaa ccatatgaaa tatatacttg aaacatgttt tggagtttga tatattttaa
     5161 taaataaagc aaggaggaag aaagtgtggg tatgttatta gcgacacgct taaaaaataa
     5221 aagaaaggaa ttaaaattat ctcaaaaaga attagctgag gggatttgtg agcaagggca
     5281 gattagccgt atggagcaag gtaaatactc acctgggtca gaattactct ttcaactatc
     5341 taagcgattg aaggtgagca tggattattt ctttgaagat actgaggttt ctagtttaga
     5401 aaatattgat aagtttaaag aattatctaa gaaatttttg gatgagaggg aatatgaatc
     5461 tctccattac atctatgaac tggaaaaatc taaacgtgct cgtctatctg ctaaagacca
     5521 gatttatttg gattggattg atacattagt tcaattctgt tatttaaatc atcaaacagg
     5581 ggcagttgcg aaactggaaa aaatactgtt atctctgaaa aaatctgatt tttactatct
     5641 acaactggga aataccttgg ctaattttta ttttgatatg gggcaggaag acacatatca
     5701 ggaactctat caacttttaa aagagaacct ccagtcacta tctgttcatc atttggaaca
     5761 gttagaattg atgattaaat tccgatataa ctattgtaga tatttgtggt tgaatgaacg
     5821 aacatctgaa gctatagaag aaattacaga aacgatagct atttgtaaaa gatataatag
     5881 tagttatcga ttagcagatt tgtattgttt attgggaagt ataagcgagg gctttgctga
     5941 aagggatagg atactaggtt tttatcaagt atcttaccag ctttacaagc acgaagagaa
     6001 ccagagaatg atgctagaaa ttgagaagga aatgaaatta ttggaaagtg aagattaagc
     6061 aaaaagattt gtattcttag ataactttaa tacatattat aaaaaaaata aaaaaatatc
     6121 ataaaaatta ttgacaaacc aaaattgatg tgttagaata tcaccaatag ttaaggaggt
     6181 ttagaagatg aaaagatctc aagttgtcaa acgatgtata ttaattgctt tattgcttct
     6241 atttctactt aatgtgttaa gtggttcttt atctgtaaca actagaggag ttgatccaag
     6301 accagcatat ggagtttcgc tttaaagttg tgcatagttc ttcttttata gacggagagt
     6361 ttttctcaat agtaagtgat tcaaattttg atggatgatt ttccattaaa ttaagtgatt
     6421 gcgaaaactt tttgaaatac attctctttg gatattgaaa gtttgttttg gattttttta
     6481 tgaagcttat cttagctgtt agattggggt tttatgggat ttgtcctcac cgggaaaaaa
     6541 tcttaaatct ttgaagaaag aggtttttat cttatggtta gagatacatc agaagaaatt
     6601 gaagttattg acttgtcttc tatgaccgtt tcctactcat ctgttatgct aggaaactct
     6661 tggatgacgt ggaattgatt tattaagata gattatttgg tttttaccaa aaatctgtct
     6721 ttttaatttt taaaggacta atggtgatat gaaactatat caaataaatg atgggataca
     6781 aatttttgaa agtagtaatc ttgaaaaaat agtaattaga aattatagga atggtaagaa
     6841 aataactatc gatcaccaac cattattaaa aaaattacta ctattccttg aagttcctaa
     6901 acgtgaagaa gaaattctat tatatcttcc agaattatca aataaaacat tacgaagtgt
     6961 tttgacgaat tttatgagtt taaatataat tcattgtcaa gatacattaa taattcacaa
     7021 aaaaataaac atattgataa taggtttagg gactacgggt ggatatctgg cagatgggtt
     7081 atgtcgtagt aatattaatt cgaagctatt tcttttagat aaggatcgtg tagatgagac
     7141 taatgtaggt agacagattt atttaagaga tgatattggt caatataaag ttgatgtatt
     7201 taaacaaaag tttagtgata aagaaataaa ggtattcaaa atttttatta atagtaagaa
     7261 agatttggaa aagatttgca aaagggaggg tatcgatttg gttatacaat gtggtgactc
     7321 cccttcacct cgagaattag gaaaaattgt gaatacagta tgtgatactc tccagatacc
     7381 ttatatagtt aacactggct acatgagtaa tgtagttcct ttgccagaat tttattatcc
     7441 aaataataga tacaatttta attataagca tcaattttca gatgaaaagt tactgttctc
     7501 acaagttttg tctaaagctg attatatggt ggcaattcaa tcatcatttg taatgttaat
     7561 gcaaataaaa aattttatat ttaatgaaga acctatttat tatggttata ggggatacta
     7621 taattctgta acattagcat gggaggtgga aaaaattgac taaatattta gaattggatt
     7681 attctcattc atatattttg gaaggtttta gaaaaaatga tgcgtttatt gaaaatcaca
     7741 aagaatgcct tgatatgtta tcagataaga tttggcgaga taataaatat atgaatacag
     7801 aaaaaaacat atcatcaccg acacgaacaa tcttatcacg ctatacttgt aatattttat
     7861 cgtctttgtt tattgatatt agcaaaaata gcatagaaaa attgatagtg acatgtgaaa
     7921 atagcgatct taattggatt tataattatt ataaatcggt ttacaatgga gtattttgtg
     7981 aattgcgaca acttatacaa cagtactgta aggtgaagga taagtatgaa aagtggcttt
     8041 ttataaaagc tattataaat caaggtattc gtcaaaatga gaaagaagtt gttgaattct
     8101 ttttaaaaca attgaaggat aataatcaag gtatttttga ttacatcaat agttttagtt
     8161 tttatctatt aaagttttat tcaaaagaca aatctcgcat ttttttagaa gagcagctag
     8221 atatagatga ttttttggga tcagataaag aagattatgc taggtcttta gtttttaaga
     8281 attatgcttc tctattgcct gatacttctg aattaaaaag aaatattatg gaaaaatgtt
     8341 taagtcaaac acctcaagat acagatttat ggaaagaatg gtttgaaact tatgccagtc
     8401 agaaagagat tagacaaaaa gcaacggaaa tatttaagca tggttattca gatatttcgt
     8461 tactaaaatt agttaaaatt gagcctgatg acactgagag tttaatcaga atgattatct
     8521 tatgtaccag tgacctaaat agtaatattg caaggtattt gatttcattt ttgagcgatg
     8581 ggcgattaaa agaatttttg gagttatttg ttgaaaattt tgactttttg aatattattg
     8641 agggaaaaac tagtgagatt aatttttaga ataattagaa aagatattat attatattct
     8701 atattaacat ttttgctatc aatagcgatg gtaagcgaag catttttgat gcaatttatt
     8761 gttgattcaa ttaaatttgg tgaaaagaag tatgttttga tttctatttt tattgtaatt
     8821 tttttgattc ttcagacttt attgtattac tatcaacaat ttcttacagc tgtattaagt
     8881 aaaaaatctg catatgagta ccgtaaatta atttttaaaa atattcagca gacttctctt
     8941 aggcttgtta caggtgaaaa aaatgatagt attctagctt ctttgacaac gcaaatcgat
     9001 caaatagaat ataattattt ttattctgtt tattggggtg gatatttgct ttgccaatta
     9061 gtggtagcta tagttatgtc gctttatcta aatccattga tgtctgtatt aaccatttgc
     9121 ttaagtctcc ctaatttatt tgtagcactt ctttttaaaa actccttgga acaaaagcaa
     9181 gaagagctaa ttaatgagac aaattcctca atagcagtta ctcaagattt aattgaggga
     9241 attactgatt ggaaaattgc aaatagagaa cctaatatat ttagagtgtt tgaaagcaaa
     9301 acattgaagc ttttagagaa gcaagtgaag gtagaaaaat ttcaatacat tgttgttagt
     9361 ttaaatcaat tgttttcaaa tgttttatat tttggtagtt ggatagtagg tggattcttg
     9421 attatacggg ggcaactttc attagggagt ttgatagcgt tctctcaatt attagcaaga
     9481 atttcttacc ctgtttacgc ttcttcggat ttattgtcaa aatatattag cggtaaaaaa
     9541 attgtagaaa ttctttctaa agaatttata ttttcagacg aaagcagaaa ttatccagat
     9601 gagtttaatg ttatttcatt tgaacattat tttacaaaca ttgacgaaaa ggatatgcct
     9661 tttgataaag aattttacaa aaataaaaaa tatttgttga aagggaaaag tggtagtggg
     9721 aaaacaacaa ttttaaaatc gattttacga gagtatgatg attatagtgg aattgtaaaa
     9781 attaatgggg tagatgttag ggatataaaa gagagtgttc tttttgacaa tatagcttat
     9841 gtttcccaaa aacctcactt gttcagcgca agtcttagag acaacctgac attattttct
     9901 gaagacttca ctgataatga gatagttgag gttttaaaat ttgttgaatt atctaaatgg
     9961 gctaataaag attgcttgga tatgatgctt tcaaatgatg atataaaatt atcaggaggg
    10021 gaggtcaaaa gggtttcttt agctcgagcc ttattgatga ataagaatat tctgttattg
    10081 gatgagttct ctgctgggat tgattatgaa acattgttaa aaatagagaa gaaactcatt
    10141 gatttggata agatgattat ttacgtgacg catgtggata tagatagatc cggcaaacag
    10201 tttgatgatg tcattgattt ggattgtctt tttatatcct cgtgacatga gagttgagtc
    10261 agttgaatta ttttgtcact ctgtttagtt cttgttggta gtgtcaggta atctttgtta
    10321 atttttcagg aagtctattg aaatatcctc ttggttatat attacgagga gtaattaatg
    10381 gatattctcg agaattcaat acaacaattt gattctaaag ttaagtatgt ttctttattt
    10441 gcggaatcaa tcttttatca gataccatga gctattttgc aggtgctatg atttctcctt
    10501 tgtatgtgag gattatcgga gatgatatga cagatgctat gggggttaga caggcaactg
    10561 ctggtgtagt taatattctc agcaacctta tagggggagt tttgattggg ggtcatcagt
    10621 cttcagatgt ttgtaggttt gaatgctttg acctttcttt ttgcactgtt gggtgtggtg
    10681 tttattcgta cttctttgag agatattgag gaacagatgg aggttagtaa aagattagcc
    10741 gcaaaatctt ttggagagca tttcctccat tcaatgaaga tattgtcaag ttttccagcc
    10801 atcatcaaat taatattcat tgcagctatg aaccaagtga ttttaagcat tattatccct
    10861 ttaacgactt tgatgttaat ccatcatcca tttatcttct ttgacactgg gaaatccatt
    10921 gcagcattgt cagtggttat tttttcagga acgattgtgg gaagtttttt gagtggaggt
    10981 atcctaaaaa atattcccat aaaaatgatt gtctactttg aagaaattat gcatctctgt
    11041 attctctttt cttggttgac caatcatttc ctccttttat tggtagttgt cttcttatgc
    11101 gcagttgcag taggaatgat ggggcctcgg attttcaacc ttgtattctc gatggttcca
    11161 gaagaagtca tgggaaccat ccagtcagtg cttggagtat tcaatgttgt tgtaccagga
    11221 attcttagca tgctcatcgt tgccttagcg agtgctacaa acctaaaaat cacagtgctt
    11281 ccacttttta tttttgtatt gactgcctta gcaattgtga aagcaatgaa aatagagtaa
    11341 tagtagtatg aactactgtc tctttttggg tgggagttca ttacccatca gacgtccttg
    11401 ccagtctctg tgtgggcttg ggagttttgt ttatcgagtt tcccttctat gacaagctcc
    11461 gcttccaatg gcgatttaaa ggcaaacaga agtgagtatc gaattccctt gaggagaaag
    11521 aaatgaaagt caatatagca gatcttcatc cgactcaact atatttatca gaaaagaagt
    11581 tgcaagatat tcagatgctt taccagtcgg cagaaacaat ccaagtcgat ccaatcagta
    11641 ttcttgcctt tggagattgt ttgctgatta cagatgggca tcacagggct tatcaggctt
    11701 tattggcagg tcgggatacg atttctgctg agtgggatag agatggtggt gatgaactat
    11761 atcatctcta tgcgcaagct tgcgaggaaa gaaagattta ctctgttttt gatttaaaaa
    11821 atcatatctt acctctttgg atcggcatgg tgagcttttt ttgattgaag tcaaggagaa
    11881 gggagaatgg tttccaattg gggatattac actatctcag gataatctcc ccattgtgat
    11941 tgggaattcc gcttaccaac atcgaggact tggaaaaaag attctaagtg ctttgattga
    12001 attggctcga gtaaaaggat ggaaagaatt gagagtcaag gaaatctaca cctacaatca
    12061 tgcttctagg aggtgtttca agtcgcttgg atttgtggaa aatggagcaa cagaaaaagg
    12121 aaggagtttt atattggaat tagtctaatc ctgccacttt ctccccctta acctttgact
    12181 attccgcaaa attatcgtaa aataaagagt aaatgataaa atgaggtcag agtctgttcg
    12241 ctctgacgat agtagtataa atgaggagaa acgctttgga attagaagta tttgctgggc
    12301 aagaaaaaag tgaactatct atgattgagg tagcgcgtgc tatattagaa cttcgtggtc
    12361 gcgatcatga gatgcatttt agcgatcttg taaacgaaat tcaaaactac cttggaacat
    12421 caaacagcga tatccgcgaa gctttgcctc tgttctacac agagttgaac tttgacggta
    12481 gcttcatctc acttggggac aacaaatggg gtcttcgttc atggtatggt gtggacgaaa
    12541 tcgacgaaga aatcatcgct cttgaagaaa atgacgacga tgaagtagca ccaaaagcta
    12601 agaaaaaacg tgtcaatgcc tttatggatg gtgattcaga tgccattgac tacaatgcag
    12661 atgatccaga agacgaagat gcatacgaag cagatccagc tctttcatac gatgatgaaa
    12721 atccagatga tgaaaaaaat gaagtggaag cttatgatgc agaaatcaac gaaatcgctc
    12781 cagatgactt gggagaagat gtggatctca acgaagacga cgacgagttt tcagatgacg
    12841 acgctgaaac cagcgaggaa taagagttag ctattgacaa ttatcctatt tttaggtatc
    12901 atattgtttg ggcacctctt ttagaggtcg gggctcccta gttcttaggg agctattttt
    12961 gttttttcaa gaagttatct tcttgtattt tatactcaat gaaaatcaaa gagcaagcta
    13021 ggaaactggc cgtaggctgc tcaaaacact gttttgaggt tgtagataag actgacaaag
    13081 tcaggaacac atatctacgg caaggcgacg ttgacgcggt ttgaagagat tttcgaagag
    13141 tattagttgt gaatctggtg cagtcgtccc agattattct tattagtagg gtcttgtttt
    13201 ctatatcccc tcgtagttaa caagaccttg agcattttag aaagaggaat ctatgtctac
    13261 gaaatatatt tttgtaactg gtggtgtggt atcgtctatt gggaaaggga ttgtggcagc
    13321 gagtctaggc cgtctcttga aaaatcgtgg tctcaaagta accattcaaa agtttgaccc
    13381 ttatatcaat attgatccgg gaaccatgag tccttaccag cacggggaag tttttgtgac
    13441 agatgacgga gctgagacag atttggactt gggtcactat gaacgtttca tcgatatcaa
    13501 tctcaacaaa tattccaacg tgacaactgg gaaaatttac agtgaagttc ttcgtaaaga
    13561 acgccgtgga gaataccttg gggcaactgt tcaagtcatt cctcatatca cagatgcttt
    13621 gaaagaaaaa atcaagcgtg ccgctctaac gaccgactct gatgtcatta tcacagaggt
    13681 tggtggaaca gtaggagata tcgagtcctt gccattccta gaggctcttc gtcagatgaa
    13741 ggcagatgtg ggtgcggata atgtcatgta tatccataca accttgcttc cttacctcaa
    13801 ggctgctggt gaaatgaaaa ccaaaccaac ccaacactct gtcaaagaat tgcgtggctt
    13861 gggaatccaa ccaaatatgt tggttattcg tacagaagag ccagctggtc aaggaattaa
    13921 aaataaactg gcccagttct gtgatgtggc accagaagcc gttatcgaat cgttggatgt
    13981 tgaacacctt taccaaattc cactgaactt gcaggcacaa gggatggacc aaattgtttg
    14041 tgatcatttg aaattagacg caccagcagc ggatatgaca gaatggtcag ccatggtgga
    14101 caaggtcatg aacctcaaga aacaagttaa gatttccctt gttggtaagt atgtggagtt
    14161 gcaagatgcc tatatctcag tggtcgaagc cttgaaacac tctggctatg tcaatgatgt
    14221 agaagttaaa atcaattggg tcaatgccaa tgatgtgaca gcagagaatg tagcagaact
    14281 cttgtctgat gcggacggga tcatcgtacc aggtggtttt ggtcaacgtg gtacagaagg
    14341 gaaaatccaa gccatccgct atgcgcgtga aaatgatgtt ccaatgttgg gagtctgctt
    14401 gggaatgcag ttgacatgta tcgagtttgc tcgtcacgtt ttaggtcttg aaggtgccaa
    14461 ttctgcagag cttgcaccag aaacaaaata ccctatcatt gatatcatgc gtgatcagat
    14521 tgatattgag gatatgggtg gaacccttcg tttgggactt tatccgtcta agttgaaacg
    14581 tggctctaag gctgctgctg cttatcacaa tcaagaagtg gtgcaacgcc gtcaccgtca
    14641 ccgttatgag tttaataatg ccttccgtga gcagtttgag gcagcaggtt ttgtcttttc
    14701 aggagtttct ccagacaatc gtttggtaga aatcgtggaa attcctgaaa ataaattctt
    14761 tgtagcttgt cagtatcacc ctgaactgtc aagccgtcca aaccgaccag aagaactcta
    14821 cactgccttt gttactgcag cggttgagaa cagcaattag caaaatcaga acctttgaga
    14881 aaaatctcag aggttttttg catacgatga tattgcaata tatctgaggt agggggcctc
    14941 tgtatgtacc tgctaccgtt gaaatcaata gcgactccct cttgccctgt gctagtgaat
    15001 ggatttatca gtatattgaa atgaaataaa atttgaacaa attaattcgg aaagccaaat
    15061 caatttctag caaagtttta ggaactggat tgtatagtga attgaaataa gatgtgaaca
    15121 tctctatcag aaaagtcaaa ttaatttata gaaatatttt agcagtcaag atgtactgtt
    15181 atagattcaa tacattatac ttttttaatt taatccacta tagtaaaatg aaataagaac
    15241 aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tgttttagaa atagaggtgt
    15301 actattctag tttcaatata ctatatatct gcagaatact ctttcagttt cattcagcct
    15361 agtcttgctt aggaaaaagt aaaaagccat ccaattatga tggatagctt cttggttctt
    15421 agatttgttc tgcaaaaacc ttttcagcaa ggttcatagc gtggtctgat acacgagtgt
    15481 agtgtgagat gatgtcgata aagttgaccc cagcttgtgt tgaacattcg cctttgttga
    15541 ggcgtttgat gtgggttttt ctgagaacac gttctatctt attgattgct tcatgacgtt
    15601 ctacaagact gcgtgctttt tcaatatcat tgttttccac actatccaga gcatctttga
    15661 taaagtcact agtttggcgg taaacttcct ctaattcttt caaggcggca tcagaaaatt
    15721 caacattttt ccgttgaaga tagtcagtca gattgagtag agcctccgtg tggtctccaa
    15781 tccgttccaa atcacgggag gaatcaagga tattggtaag cacttcactt tctttttggc
    15841 tgagagcttc gcttgaaagg gcaatgagat aacgtgttaa ttgctcatcg atggtgttaa
    15901 ttgcttcttc ggttttatgc cctttttcag caactttttc atccaagtca atgatgtact
    15961 tataggaaag gtcaaaggct ttagcagcgt agtttcctaa gtgcaagagc tctttcttag
    16021 catttcctag agcgatagat ggggcctgtt tgatgaaatg ttcatcaaga tataagggtt
    16081 cgtatttgac aacctcgtcc tctccaggaa taatcttggt tacaaagtaa gccagagctc
    16141 cgataaatgg aaattggaca atggtgttgg taatattaaa ggttccgtga gcaaaggcga
    16201 tggtcatttc cggtgctaga tttagcgtag cttcaaacca atgaatcagg acagtaaaag
    16261 gaactagaaa aataacgcag acgactgttc cgataacgtt gaaggcaaca tgagctcctg
    16321 ctacccgttt agctgcaata ttagccccta aagaggcaat gatggctgta atggttatcc
    16381 cgatattgtc accaaataga actggcaaag ctccctgtag gtcaattaga ttgccggcgt
    16441 agaggttttg taaaatccca atggtagccg aagaagcttg aattagcaag gtcaagccgg
    16501 taccgacaaa gacacccaaa acaggattct tacttagctc aatcatatag tccttaaaga
    16561 cctgtaaatc cttgagtgga gccattgcgc cgctcatgag attgagggca aaaaagatac
    16621 caccgacacc aaagaggatg cgtccgatat tattgactgt ccgatttttt gtaaaaaaga
    16681 gacagacggc accgataaag agcataggta gggcatagtt acctaattta aaaccgatga
    16741 gaaaggatgt gacagttgtc ccaatattag cacccatgac aatcccgata gcctgacgta
    16801 aggttaagag accggcactg accaggccga ctgtgataac tgttacacca gaactagact
    16861 gaattagagc agtcatccca ataccaacca gaactccaaa gaaaggatta ctagtatatt
    16921 tgtcaatgta aaaacgaagg cgatctccag cagcttgttg caaaccgtct cccatggtct
    16981 tgatgctata taagaatagc cccagaccac ctaaaaagtg aaataaaatt tcctgccaat
    17041 taatggacat ttctttttcc tccgaaaaat aatcgcggaa tatcttctat tctattttaa
    17101 aggataaaag taaatctaac aagtgttaat gtcaagtttg taaaagaaaa ttataattga
    17161 taagtgtatt atttttaaga gtttttaacc ttattttaga tttgtgcaaa cagttattgt
    17221 tttttgtcta aaaatgtttt aggataaaaa tgtgattatc atggttctag agtttgataa
    17281 aaaactacag aatataaagc gctttcttga aaacaacaaa atcaatcttt taggaggaga
    17341 aaatgaagaa tccatttttt gaaagacgtt gtcgttacag tattcgtaag ttatcagtag
    17401 gagcctgctc gctgatgatt ggtgctgttt tatttgctgg tccagccttg gctgaagaaa
    17461 ctgcagttcc tgaaaatagc ggagctaata cagagcttgt ttcaggagag agtgagcatt
    17521 cgaccaatga agctgataag cagaatgaag gggaacatgc tagagaaaac aagctagaaa
    17581 aggcagaagg agtagcgata gcatctgaaa ctgcttcgcc agcaagcaat gaagctgcaa
    17641 ctactgaaac tgcagaagca gctagcgcag ctaaaccaga ggaaaaagca agtgaggtgg
    17701 ttgcagaaac accatctgca gaagcaaaac ctaagtctga caaggaaaca gaagcaaagc
    17761 ccgaagcaac taaccaaggg gatgagtcta aaccagcagc agaagctaat aagactgaaa
    17821 aagaagtcca gccagatgtc cctaaaaata cagaaaaaac attaaaacca aaggaaatca
    17881 aatttaattc atgggaagaa ttgttaaaat gggaaccagg tgctcgtgaa gatgatgcta
    17941 ttaaccgcgg atctgttgtc ctcgcttcac gtcggacagg tcatttagtc aatgaaaaag
    18001 ctagcaagga agcaaaagtt caagccttat caaacaccaa ttctaaagca aaagaccatg
    18061 cttctgttgg tggagaagag ttcaaggcct atgcttttga ctattggcaa tatctagatt
    18121 caatggtctt ctgggaaggt ctcgtaccaa ctcctgacgt tattgatgca ggtcaccgta
    18181 acggggttcc tgtatacggt acactcttct tcaactggtc taatagtatt gcagatcaag
    18241 aaagatttgc tgaagctttg aagcaagacg cagatggtag cttcccaatt gcccgtaaat
    18301 tggtagacat ggccaagtat tatggctatg atggctattt catcaaccaa gaaacaactg
    18361 gagatttggt taaacctctt ggagaaaaga tgcgccagtt tatgctctat agcaaggaat
    18421 atgctgctaa ggtaaaccat ccaatcaagt attcttggta cgatgccatg acctataact
    18481 atggacgtta ccatcaagat ggtttgggag aatacaacta ccaattcatg caaccagaag
    18541 gagataaggt tccggcagat aacttctttg ctaactttaa ctgggataag gctaaaaatg
    18601 attacactat tgcaactgcc aactggattg gtcgtaatcc ttatgatgta tttgcaggtt
    18661 tggaattgca acagggtggt tcctacaaga caaaggttaa gtggaatgac attttagacg
    18721 aaaatgggaa attgcgcctt tctcttggtt tatttgcccc agataccatt acaagtttag
    18781 gaaaaactgg tgaagattat cataaaaatg aagatatctt ctttacaggt tatcaaggag
    18841 accctactgg ccaaaaacca ggtgacaaag attggtatgg tattgctaac ctagttgcgg
    18901 accgtacgcc agcggtaggt aatactttta ctacttcttt taatacaggt catggtaaaa
    18961 aatggttcgt agatggtaag gtttctaagg attctgagtg gaattatcgt tcagtatcag
    19021 gtgttcttcc aacatggcgc tggtggcaga cttcaacagg ggaaaaactt cgtgcagaat
    19081 atgattttac agatgcctat aatggcggaa attcccttaa attctctggt gatgtagccg
    19141 gtaagacaga tcaggatgtg agactttatt ctactaagtt agaagtaact gagaagacca
    19201 aacttcgtgt tgcccacaag ggaggaaaag gttctaaagt ttatatggca ttctctacaa
    19261 ctccagacta caaattcgat gatgcagatg catggaaaga gctaaccctt tctgacaact
    19321 ggacaaatga agaatttgat ctcagctcac tagcaggtaa aaccatctat gcagtcaaac
    19381 tatttttcga gcatgaaggt gctgtaaaag attatcagtt caacctcgga caattaacta
    19441 tctcggacaa tcaccaagag ccacaatcgc cgacaagctt ttctgtagtg aaacaatctc
    19501 ttaaaaatgc ccaagaagcg gaagcagttg tgcaatttaa aggcaacaag gatgcagatt
    19561 tctatgaagt ttatgaaaaa gatggagaca gctggaaatt actaactggc tcatcttcta
    19621 caactattta tctaccaaaa gttagccgct cagcaagtgc tcagggtaca actcaagaac
    19681 tgaaggttgt agcagtcggt aaaaatggag ttcgttcaga agctgcaacc acaacctttg
    19741 attggggtat gactgtaaaa gataccagcc taccaaaacc actagctgaa aatatcgttc
    19801 caggtgcaac agttattgat agtactttcc ctaagactga aggtggagaa ggtattgaag
    19861 gtatgttgaa cggtaccatt actagcttgt cagataaatg gtcttcagct cagttgagtg
    19921 gtagtgtgga tattcgtttg accaagccac gtaccgttgt tagatgggtc atggatcatg
    19981 caggagctgg tggtgagtct gttaacgatg gcttgatgaa cactaaagac tttgaccttt
    20041 attataaaga tgcagatggt gagtggaagc tagctaagga agtccgtggt aacaaagcac
    20101 acgtgacaga tatcactctt gataaaccaa tcactgctca agactggcgc ttgaatgttg
    20161 tcacttctga caatggaact ccatggaagg ctattcgtat ctataactgg aaaatgtatg
    20221 aaaagcttga tactgagagt gtcaatattc cgatggccaa ggctgcagcc cgttctctag
    20281 gcaataacaa ggtacaagtt ggctttgcag atgtaccggc tggagcaact attaccgttt
    20341 atgataatcc aaattctcaa actccgctcg caaccttgaa gagcgaagtt ggaggagacc
    20401 tagcaagtgc accattggat ttgacaaatc aatctggtct tctttattat cgtacccagt
    20461 tgccaggcaa ggaaattagt aatgtcctag cagtttccgt tccaaaagat gacagaagaa
    20521 tcaagtcagt cagcctagaa acaggaccta agaaaacaag ctacgccgaa ggggaggatt
    20581 tggaccttag aggtggtgtt cttcgagttc agtatgaagg aggaactgag gacgaactca
    20641 ttcgcctaac tcacgcaggt gtatcagtat caggttttga tacgcatcat aagggagaac
    20701 agaatcttac tctccaatat ttgggacaac cagtaaatgc taatttgtca gtgactgtca
    20761 ctggccaaga tgaagcaagt ccgaaaacta ttttgggaat tgaagtaagt caggaaccga
    20821 aaaaagatta cctagttggt gatagcttag acttgtctga aggacgcttt gcagtggctt
    20881 atagcaatga caccatggaa gaacattcct ttactgatga gggagttgaa atttctggtt
    20941 acgatgctca aaagactggt cgtcaaacct tgacgcttcg ttaccaaggt catgaagtca
    21001 actttgatgt tttggtatct ccaaaagcag cattgaacga tgagtacctc aaacaaaaat
    21061 tagcagaggt tgaagctgct aagaacaagg tggtctataa ctttgcttca ccagaagtaa
    21121 aagaagcttt cttgaaagca attgaagcgg ccgaacaagt gttgaaagac catgaaacta
    21181 gcacccaaga tcaagtcaat gaccgactta ataaattgac agaagctcat aaagctctga
    21241 atggtcaaga gaaatttaag gaagaaaaga cagagcttga tcgtttaaca ggtgaggttc
    21301 aagaactctt ggctgccaaa ccaaaccatc cttcaggttc tgccctagct ccgcttcttg
    21361 agaaaaacaa ggccttggtt gaaaaagtag atttgagtcc agaagagctt gcaacagcga
    21421 aacagagtct aaaagatctg gttgctttat tgaaagaaga caagccagca gtcttttctg
    21481 atagtaaaac aggtgttgaa gtacacttct caaataaaga gaagactgtc atcaagggtt
    21541 tgaaagtaga gcgtgttcaa gcaagtgctg aagagaagaa atactttgct ggagaagatg
    21601 ctcatgtctt tgaaatagaa ggtttggatg aaaaaggtca agatgttgat ctctcttacg
    21661 cttctattgt gaaaatccca attgaaaaag ataagaaagt taagaaagta tttttcttac
    21721 ctgaaggcaa agaggcagta gaattggctt ttgaacaaac ggatagtcat gttatcttta
    21781 cagcaccaca ctttactcat tatgcctttg tttatgaatc tgctgaaaaa ccacaacctg
    21841 ctaaaccagc accacaaaac aaagtccttc caaaacctac ttatcaaccg gcttctgatc
    21901 aacaaaaggc tcctaaattg gaagttcaag aggaaaaggt tgcctttcat cgtcaagagc
    21961 atgaaaatgc tgagatgcta gttggggaac aacgagtcat catacaggga cgagatggac
    22021 tgttaagaca tgtctttgaa gttgatgaaa acggtcagcg tcgtcttcgt tcaacagaag
    22081 tcatccaaga agcgattcca gaaattgttg aaattggaac aaaagtaaaa acagtaccag
    22141 cagtagtagc tacacaggaa aaaccagctc aaaatacagc agttaaatca gaagaagcaa
    22201 gcaaacaatt gccaaataca ggaacagctg atgctaatga agccctaata gcagtcttag
    22261 ccagccttgg tcttgctagt ttagccttga ccttgagacg gaaaagagaa gataaagatt
    22321 aaatatcgaa aaatcttgtg aaatctttcc ttatatttcc aaagtgtgat ataatagttt
    22381 cgaataaaat aaataaagga gtttttgtaa catggcaaaa cttactgtta aagacgttga
    22441 cttgaaaggt aaaaaagtcc tcgttcgtgt tgacttcaac gtaccattga aagatggcgt
    22501 aatcactaac gataaccgta tcacagcagc tcttccaact attaagtaca tcatcgaaca
    22561 aggtggacgt gcaattcttt tctctcacct tggacgtgtg aaagaagaag ctgataaagc
    22621 tggcaaatca cttgctcctg tagcagctga cttggcagca aaacttggtc aagatgttgt
    22681 tttcccaggt gtcactcgtg gtgctgaatt ggaagcggca atcaacgctc ttgaagatgg
    22741 acaagttctc ttggttgaaa acactcgtta cgaagatgtt gacggcaaga aagaatctaa
    22801 aaacgatcct gaacttggta aatactgggc atcacttgga gatggtatct tcgtaaacga
    22861 tgcattcggt acagctcacc gtgcacacgc atctaacgtt ggtatctcag caaacgttga
    22921 aaaagcagtt gctggtttcc ttcttgaaaa cgaaattgcc tacatccaag aagcagttga
    22981 aactccagaa cgtccattcg tggctatcct tggtggttca aaagtttcag acaagatcgg
    23041 tgttatcgaa aacttgcttg aaaaagctga taaagtcctt atcggtggtg ggatgactta
    23101 cacattctac aaagcacaag gtatcgaaat cggtaactca cttgtagaag aagacaaatt
    23161 ggatgttgcg aaagctcttc ttgaaaaagc aaatggtaaa ttgatcttgc cagttgactc
    23221 aaaagaagct aacgcatttg ctggttacac tgaagtgcgt gacactgaag gtgaagcagt
    23281 ttctgaaggc ttccttggtc ttgacatcgg tccaaaatct atcgccaaat ttgacgaagc
    23341 tttgactggt gccaaaacag ttgtatggaa cggacctatg ggtgtatttg aaaacccaga
    23401 tttccaagct ggtacaatcg gtgtgatgga cgctatcgtg aaacaaccag gagttaaatc
    23461 aatcatcggt ggtggtgact cagctgccgc agcgattaac cttggccgtg cagacaagtt
    23521 ctcatggatt agtacgggtg gtggagcatc aatggaactt cttgaaggta aggttcttcc
    23581 aggacttgca gccttgacag aaaaataaga ttttataaac aaatcaaaga agagagggat
    23641 gaaagttcct cttttctttt gcttaaaata aaaacgcttc ctctcaacta ttactcataa
    23701 aaatcaccga tttatgataa aatggaaata gaaagttgag attatgagtt attttaaaaa
    23761 atataaattc gataaatccc agttcaaact tggtatgcga acctttaaaa caggtattgc
    23821 tgtttttcta gttctcttga tttttggctt ttttggctgg aaaggtcttc aaattggtgc
    23881 tttgacagcc gtttttagcc tgagggagag ttttgatgag agtgttcatt ttgggacttc
    23941 gcgtattcta ggaaatagta tcggtggact ctatgccttg gtcttcttct tattaaatac
    24001 ttttttccac gaagcctttt gggtgacctt ggtagttgtt ccaatctgca ccatgttaac
    24061 cattatgaca aatgtagcca tgaataacaa agcaggggtt attggtggtg tagcagctat
    24121 gttaatcatt accctatcaa ttccaagtgg tgagacaatt ttgtacgtgt ttgtgcgtgt
    24181 attagaaacg tttatgggag tttttgtcgc aattatcgta aattacgata ttgatcgtat
    24241 tcgtctcttt ttagagaaaa aagaaaaata atgttacatt ttataacatt atcaattgac
    24301 gtttgtcttt ttttagacta taatagacag aaagaaggaa attgtaaatg aaggaaaaag
    24361 aatttcgccg aaatatggct gtttttccta tcggcagtgt tatgaagttg accgatttat
    24421 cggcgcgtca gattcgttat tatgaagatc aagagttgat caagcccgat cgaaacgaag
    24481 gaaatcgtcg catgtattcc ttgaatgaca tggatcgtct gcttgaaatc aaagattata
    24541 tctctgaagg ttataatatc gctgccatta agaaaaaata tgctgaacgt gaagcgaaat
    24601 ccaagaaagc ggttagtcag actgaagtac gtcgtgcact tcacaatgaa ctcctccaac
    24661 aggggcgttt tgcttcagta cagtcacctt ttggtcgcgg ttaggcaacc gcaagtagtc
    24721 atagattaag gagaaaactt atgccaatca cagctgcaga tattcgtcgt gaagtcaagg
    24781 aaaaaaatgt tacctttatt cgtcttatgt tctcagatat tttgggaacc atgaaaaacg
    24841 tcgaaattcc tgctacagat gaacagttag ataaggtctt gtcgaacaag gttatgtttg
    24901 atggatcttc tattgaaggt tttgtacgta tcaatgagtc ggatatgtac ttgtacccgg
    24961 acttggatac atggacagtc ttcccttggg gagatgaaaa tggaagtgtt gcaggtctga
    25021 tctgtgatgt ctatacaaca gaaggtgaac catttgcggg tgaccctcgt ggtaatttga
    25081 aacgagctct tcgtcacatg gaagaagttg gattcaaatc cttcaacctt ggtccagagc
    25141 cagaattctt cctatttaag ttggatgaaa atggggaccc aacacttgaa gtgaatgaca
    25201 agggtggcta ctttgatttg gcacctactg accttgcgga caacacacgt cgtgagattg
    25261 tgaatgtctt gaccaaaatg ggatttgaag tagaagcgag tcaccacgag gttgcggttg
    25321 gacagcatga gattgacttt aagtacgatg aagttctccg tgcttgtgat aagattcaaa
    25381 tctttaagct tgttgttaaa accattgctc gcaaacacgg actttacgca acatttatgg
    25441 cgaagccaaa atttggtatt gctggatcag gtatgcactg taatatgtcc ttgtttgatg
    25501 cagaaggaaa taacgccttc tttgatccaa atgatccaaa aggaatgcag ttgtcagaaa
    25561 cagcttacca tttcctaggc ggtttgatca agcatgctta caactatact gccatcatga
    25621 acccaacagt taactcatac aaacgtttgg ttccaggtta tgaagcgcct gtttacattg
    25681 cttgggctgg tcgtaaccgt tcgccacttg tgcgcgtacc tgcttcacgt ggtatgggaa
    25741 ctcgtcttga gttgcgttca gtggatccaa tggcgaaccc ttacattgct atggctgttc
    25801 ttttggaagt tggtttgtat ggtattgaaa ataaaatcga agcaccagct cctatcgaag
    25861 aaaatatcta catcatgaca gcagaagagc gcaaggaagc tggtattaca gaccttccat
    25921 caactcttca caacgctttg aaagctttga cagaagatga agtggttaaa gctgctctcg
    25981 gagatcacat ctacactagc ttccttgaag ccaaacgaat cgaatgggca agttatgcaa
    26041 ccttcgtttc acaatgggaa attgataatt atttagacct ttactaatac taatatagaa
    26101 gaaagattgc ctgtgggtaa acagtcacac agcagtctaa gccatcaaat acctataacc
    26161 cttaaaatag cgatttcggt cgctatttat ttttcgaaaa atcctcttga ctcttttgcg
    26221 aattagattt atcatcagac gtgtagaaga aaatcaagga gtcactatgg aaaaattcaa
    26281 tttcaaaaac aatatcgggc aagaaaacaa gttacttcaa atcgaaatct acaagtttac
    26341 taacttttgc aagcttcaaa attacacatc tgtaaatatt ttttcgaaag atatatttga
    26401 agccatagta aattgagact agaatagtac actacatctg ctaaaaattt ctagaaatta
    26461 atttaacttt cctaatcgat ttgtttacat cttatttcaa tctactatat tatctcaata
    26521 aatgatgatg gcgactgttt tgtttgtgct tgtcgatttt tgaatcctga tcgagcggaa
    26581 gaatatttct gccgaggttg tcaaggggaa aatcctgaag atattgaatt ttatgatgaa
    26641 caattacagg cagaaaaggt ggaggctttg aatattcgat tggaagtaaa aaattgaatt
    26701 atctacaatc tcatcaacaa taaacctatt tcaaccccat tatctatagg cgtattttta
    26761 cgccttttta tatacttgaa gcacataaga aacattatgg gaagtttgct gatggaagta
    26821 ctcaagaaat tgatgttcct tatgatattc ctgatacttg ggagtgggtg aggataaaat
    26881 caatttattg gaattttggg caaaataagc cagagaaatc ctttaggtat atagatacgt
    26941 ctagtattga tagaaaaaag aacataatca actacaaaaa tctacaatat ctttcacctg
    27001 aacaagcgcc ttcccgtgcc agaaaattag tttcgcagaa tagtgtctta ttttcaacag
    27061 ttagaccata tctaaaaaat attgctgtag ttagagaact taaagagtat ttgatagcta
    27121 gtacagcatt tattgttttg gatactttac ttaacgaaac atatttgaaa tattatttat
    27181 tatcagataa ttttatcaat cgtgttaata ataaatctac tggaacaagt tatcctgcaa
    27241 tcaatgatta taattttaat ctattattaa tt
//
LOCUS       DAVBCB010000027        26076 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVBCB010000027 DAVBCB010000000
VERSION     DAVBCB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 26076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.027"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..183
                     /locus_tag="VQU28_001106"
     CDS             <1..183
                     /locus_tag="VQU28_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476467.1"
                     /translation="LTVEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFG
                     LRMNLSAGIINHELGF"
     gene            complement(346..597)
                     /locus_tag="VQU28_001107"
     CDS             complement(346..597)
                     /locus_tag="VQU28_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476468.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHAFFMDNVEWSHADSYFLS
                     FVSDDVEERYIENVYLDSLSVKQKFKFIFDFGDEWRFEC"
     gene            complement(887..2767)
                     /locus_tag="VQU28_001108"
     CDS             complement(887..2767)
                     /locus_tag="VQU28_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HET2476469.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(2761..3630)
                     /locus_tag="VQU28_001109"
     CDS             complement(2761..3630)
                     /locus_tag="VQU28_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2476470.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPDLENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNILCQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQDVKKAVDNFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(3714..6359)
                     /locus_tag="VQU28_001110"
     CDS             complement(3714..6359)
                     /locus_tag="VQU28_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HET2476471.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDERFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELIVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFALECHQAQERFSAFRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVFRYYNMSQENVHISEHQQTILDLLE
                     RPYPVHSGPLDPQEIRTEFIKKEEI"
     gene            complement(6450..7730)
                     /locus_tag="VQU28_001111"
     CDS             complement(6450..7730)
                     /locus_tag="VQU28_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HET2476472.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIENYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSIDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            7901..9985
                     /locus_tag="VQU28_001112"
     CDS             7901..9985
                     /locus_tag="VQU28_001112"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HET2476473.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLNQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEAGDWRIDLASSQTEMQLATSFIS
                     PSQALINLPQEDFDSCKTSAQADWENLLHRFDIIETGEADRTFFDHCLYRLFLFPQTF
                     YEVDESGKAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIASCAKKLENIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKENKWLDIHTEQNHSHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPNH"
     gene            complement(10025..11704)
                     /locus_tag="VQU28_001113"
     CDS             complement(10025..11704)
                     /locus_tag="VQU28_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HET2476474.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFDCRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            12459..13688
                     /gene="arcA"
                     /locus_tag="VQU28_001114"
     CDS             12459..13688
                     /gene="arcA"
                     /locus_tag="VQU28_001114"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HET2476475.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELILSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGNLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            13746..14762
                     /gene="argF"
                     /locus_tag="VQU28_001115"
     CDS             13746..14762
                     /gene="argF"
                     /locus_tag="VQU28_001115"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HET2476476.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            14927..15874
                     /gene="arcC"
                     /locus_tag="VQU28_001116"
     CDS             14927..15874
                     /gene="arcC"
                     /locus_tag="VQU28_001116"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HET2476477.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVIVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIVLTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            16083..17594
                     /locus_tag="VQU28_001117"
     CDS             16083..17594
                     /locus_tag="VQU28_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HET2476478.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYEA
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            17616..18947
                     /locus_tag="VQU28_001118"
     CDS             17616..18947
                     /locus_tag="VQU28_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HET2476479.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAEDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            complement(19353..19643)
                     /locus_tag="VQU28_001119"
     CDS             complement(19353..19643)
                     /locus_tag="VQU28_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476480.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
     gene            complement(19631..19882)
                     /locus_tag="VQU28_001120"
     CDS             complement(19631..19882)
                     /locus_tag="VQU28_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HET2476481.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGDQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            complement(20281..21165)
                     /locus_tag="VQU28_001121"
     CDS             complement(20281..21165)
                     /locus_tag="VQU28_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2476482.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            complement(21310..22461)
                     /locus_tag="VQU28_001122"
     CDS             complement(21310..22461)
                     /locus_tag="VQU28_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HET2476483.1"
                     /translation="MAIFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHRCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPQALTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIEHENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIVEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            complement(22607..24373)
                     /locus_tag="VQU28_001123"
     CDS             complement(22607..24373)
                     /locus_tag="VQU28_001123"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HET2476484.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEANDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRG
                     IYDPEEFERAMVWVKEHIKEGVDRNREDLILSKEEKEKQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGCAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDSGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            complement(24430..>26076)
                     /locus_tag="VQU28_001124"
     CDS             complement(24430..>26076)
                     /locus_tag="VQU28_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HET2476485.1"
                     /translation="NYRTDKTAMWALSGNFDASKSWKKEELELANWISKNYSINPVDN
                     DFRTTTLTLKGHTGHKPQINISGDKNHYTYTENWDENTHVYTITVNHNGMVEMSINTE
                     GTGPVPFPTPDKFNDGNLNIAYAKPTTQSSVDYNGDPNRAVDGNRNGNFNSGSVTHTR
                     ADNPSWWEVDLKKMDKVGLVKIYNRTDAETQRLSNFDVILYDNNRNEVAKKHVNNLSG
                     ESVSLDFKEKGARYIKVKLLTSGVPLSLAEVEVFRESDGKQSEEDIDKITEDKVVSTN
                     KVATQSSTNYEGVAALAVDGNKDGDYGHHSVTHTKADSNAWWQVDLGEEFTVSKVDIY
                     NRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDKVVDGLLSLKVPSVGAKLVKIELKSAA
                     IPLSLAEVEAYGSKRTPKKLSNIALTKETRQSSTDYNGFSRLAVDGNKNGDYGHHSVT
                     HTKGDSPSWWEIDLAQTEELEKLIIYNRTDAEIQRLSNFDIIIYDSNNHEVFKQHIDS
                     LESNNLSIDLKGLKGKKVRISLRNAGIPLSLAEVEVYTYK"
BASE COUNT         7403 a         5824 c         5085 g         7764 t
ORIGIN      
        1 ctaacagttg aagataaagc ctataaccat gcgctatcca aggagagaag caaggttgag
       61 aacatctttg ccaaagtaaa aacgtttaaa atattttcaa caacctatcg aaatcatcgt
      121 aaacgcttcg gattacgaat gaatttgagt gctggtatta tcaatcatga actaggattc
      181 tagttttgca ggaagtctat tgaaatacta agcctacaca gactgatttc aattttacca
      241 ttcttcatag tcaaaaccat cataatctgg atattgttct ggcaacgttc caaccgaacg
      301 tacgagataa gcttcttcgt cctctgtctc gatttctctc agcacttagc attcaaaacg
      361 ccattcatca ccgaagtcga aaataaactt aaatttttgt ttgacactta ggctatccag
      421 atagacattt tctatgtaac gttcttcaac atcgtcacta acaaagctaa gaaagtaaga
      481 atctgcatga ctccactcaa cattatccat gaaaaatgca tgagcatgat catttgcaaa
      541 atcgaaactc cataaaatca catctgctag ttccgctaac gtttttgttt ttggaatcta
      601 tatatgtcgg tagcaacctt ttttaatttg acggaaatga tccaagcttc tacagatgct
      661 ggcttttcag cttctatttc ttgttttact aattttaact gtagcatttg aaaccagata
      721 attcttctgt ttcacgcgaa acaccctgcg ccaagcaagg tgttcttgtt tttatactca
      781 atgaaaatca aagagcaaac taggaagcta gctgcaggct gctcaaaaca ctgttttgag
      841 gttgtagatg gaagctgacg tggtttgaag agattttcga agagtattaa gtcgtataaa
      901 tcgtcgaagc tgtcgcaatg gtatgccact ggttagctgt agttgctgcg aaatccttgt
      961 ctgcgtagaa gtcagtaaat ggtaggattt caacttccag ctcgtcgatg cggtcaagct
     1021 ggccagccag ataaacctcg atacggcttt ctgctcgttt gatgcgttgc aagagtccgc
     1081 ccatacggat gtcaactgta tccaaaccaa agaccttgtt ttctttcagc cattggtgac
     1141 taaagagggc atggaagtct tcaatttggc ttctaagttc tggtaattct tgtctggcga
     1201 tttgttgtaa actttcttta tcatccgctt ggtaggcctg acgaatgcgt cgtcccacat
     1261 ctactttgct acttaaaata gcattcaact gggcctgagt ttcaaagaga taagcatagt
     1321 ttccagcttt ttctttaatg ttagcaagcg tctcagcagc ctgagcgaag tgcggtttgt
     1381 cctgttcagg tgtcatgtgt tgatcaagaa tcggacaaag aatatcctga taaaaaacat
     1441 agcggttggg attgataccg ctgagattgc ctggtaggtc tggtaagagg ttggcaaggt
     1501 caatctgcat aaaatcctca accgttagac cagtattggt cttgaagtgc gcagacaaac
     1561 catctaggtc attgcgatag ctgagttctg cccagatttg caagcttggt aggatagaga
     1621 actgggcagt ttcaccacca ttgtctcccc aacccgttac gatgacttct ttaatctgat
     1681 tggcacggca ggctttatta gcctcgatag ccactagacg gctaaaatgg ttgtgaggtg
     1741 taaagccaat ccacttccaa gctccccctg caaatgcaag gtcatggcta atcttgtgat
     1801 gattgcggaa attacggttg tatttttcct cgctatcctg ataataatcc cagtaaacca
     1861 gagtcacacg gtctttgaga cggtctaggt agacacgagt ttcctctgga atttccacat
     1921 cacggtcgta ctggccatcc gctgacatga gtttgaagaa catatcactc cacatctggc
     1981 agtggaaacc atatttgtca gcaatatcca gcacgcgctc caagtgttgg cacatgagga
     2041 gactacgatc cacaacaccg ttcaaaatca ggtagcgtcc caaaccaacc aagtgggctt
     2101 cgtccatccc gatattgacc ttgcgagtct tcagtttaga caacgtggca aacatgccat
     2161 caatcaggtc ataaaccttt tcttcgccaa tgagaagaat gtcctctaca tcacggagct
     2221 cctgcacttc cttgacaccc catttgacaa aggccgacaa gtgggccaag gtctggatgc
     2281 atggtacaaa ggtcatgtca aactgttgag cataggcttc gatttcctgc aactcctctg
     2341 ctgaataagc tccacggaaa tagccaaagt aaggctgccc ttcaatctgg taagtgtctt
     2401 ccatgtaaag ttcaaaggtt gagtagccca tgagagccaa gacctcaatc atctgcttgg
     2461 cagaagccac attcagcacc gcatttcgag aacagtcaac catgtaagcc aaatcttcgt
     2521 aagctgcttg ttcctcaatc tctactttat cagcttctgc tagaactgtt accaacaagg
     2581 ataaggcacg ataaagttgg tgaggtttgc ggtaggtcaa ttgatagtga ccttcctcac
     2641 cctggataga gatagaggct tggtcagact gagtgacagc cacttccaca tctggtagag
     2701 aaatatgacc ttttaaaacc tcaatagctt gcgtttgttt gggactaagt cctgtaaatc
     2761 ttaccattgc ttttcctcct gtagccagtt gacaagagca ccgtagagat tggcatctgc
     2821 gtgataggtg caggcctgga taactggtgc gaccgtgtat tcttcgtagg tatcgacaaa
     2881 gttatcaaca gccttcttga catcttggat aaaatctgga ttttgactga tagagccacc
     2941 cagactgatg acatctggat cgatcagata ctggatattg agcaagcctt gcgccagatt
     3001 gcggttcatg cgctcaatgg cttcttgaca aaggatatta ccagctgcgg cctcttggta
     3061 aatcttgcga ccgtcccaat cagtatgacc agatttttca atcacgtatc gtaccatatt
     3121 cccagttgat gctagttgcg accagttatt aagtttttca gcaggggcaa gggttgtcat
     3181 gtagccaaat tctccaccca agccgtggcg acctcgatga agtctaccat tgataatcat
     3241 ggctccgcca atccctgtcc caatcacgac acaggctgca ttttcaagat ctggatgagc
     3301 tagtagttca ctgagtccaa cgcagttggc atcattttct aaatggacag gtagctgata
     3361 agagctaagc gcctcatacc aagaaaagcc atggatgtag ggcaccgcac tgaagccatc
     3421 aatcacacct gtctcttgat tgactgcacc tggaacgctc atagcaatcc cactgtaatc
     3481 ctgttctgac aagcgttgat ctagccacgc tagtaaatcc tccaagtttt caggcgttga
     3541 aatacttgtc ttatccagta ttttcccatc aggagtcaga ctggcaaact taatcccagt
     3601 ccctccgata tcaatcgttg caatggtcat tgtttttacc ataaaaaggg gcgaatcagc
     3661 agttagttct tactttttcg cccctccttt cttttgatgt ttactttttg aaattaaatt
     3721 tcttcttttt tgatgaattc tgtacgaatc tcttgtggat ccaatggtcc tgaatgaact
     3781 ggatatggtc gctcaagtaa gtcaagaatg gtttgttgat gttctgatat gtgcacattt
     3841 tcttgactca tattatagta acggaagaca tagccttctt cattttccgc cactttaaag
     3901 gctgttggac aaacttgcgg tatgctgaga acagaatggc tcaagaggct accagtcgca
     3961 accacgcttc cttcctgtct agcaagctga aggctggtaa acggtgtctg caaggctttg
     4021 gcacgacgga aagctgaaaa gcgttcttgg gcttggtggc attcaagtgc aaactcgact
     4081 tcaaactcac gcaagcattg tgcttctggc gttgggaagt agccccagtc acctagctca
     4141 cctgatgcac gcaaaatggt cacggcaatg gtgttatccc caaggatttc gtattcattc
     4201 aatcccttat tggatacagt cacccctttt tcatcgtcat acagactgac aaaagcttgt
     4261 tggtgttgag gattttcagg attttcccat gaagcagctg gtttgtttgg tcgtgtcacc
     4321 acctcataga tactttcaga atcattgctt ggacgcgtgt tatgagtctt gaccaagaga
     4381 cggatacggt gatccttggc agtgttagta aagcgagtct tgaagcggat ttgtggattg
     4441 tcaacaaaga caatcaactc agtttccaga ggaatgtttg tcaattcttc tgaccgtcca
     4501 gcctcacgct tcataaactc gatgatacct tgttgctctt cttctagctt ttcatccgca
     4561 ctgacaggca cggtcaattc atgtttgagc aagattttag catagcgatc tgtgttttcc
     4621 aagacctcgt ggcccttaag ctctgcaaag attggctctg ttccttttgg ttggaaatag
     4681 atatactcgt ttccgatgtc cccacggtct tcgaagcgga taaagtcttc ataggcttcg
     4741 tgagttgtct tgtcatatac tgtgatattg tcatccacac tcaccgttac gaatggtgta
     4801 tcaatcactc cgttttggta aataccctca cggtgttctt gttttccttc cagcaattgg
     4861 aaggttgtcc aagaaagcgg cgctaggtga actggaatgg tcacgcgcac ttgtcgagca
     4921 atacgagctt ggcggaactt gtcttttggt aaatcgtact caaaattagc tccgaggtct
     4981 tcgattgtag cctctacagg acgaccatcc aagtcctcca cacggtaact tggtaagata
     5041 agagcagcca tctttttgta gccttctgtt gggtgcaatt ccttgaaatc acaagtcgcc
     5101 acatcaatca ctgtgctgac agtatcgacc ttatcatgca agcctgtgtt aatgacagta
     5161 aagagatagt cactttgagc cttatccgta gcaattttac ccttccactc gttgagcaag
     5221 ttacttttaa caaagtttcc tacttggttg accttggcaa aacgcgtttc catctcgcgg
     5281 tgaacttcgt ccacgctaca gccacagata ctatcatgtg gcgcattctg caaaagtgtt
     5341 ttccaagcat aggtcaactg gtccttgtgg ttgtgtccac cagtgataat agtcaagggt
     5401 tctaccactt gctctaggag gttgctattt tcttggaagg cttgttttag gtaaatgcgg
     5461 gatgaagaag tgttggcaag tgtgtaccag ccatctgttt cctgactggt caactcgcct
     5521 gtaacagttg ataagtgttc aggaagcgca ccttctacag cttgaacata ttcatcaaaa
     5581 gaactatgaa caaagattac atccgggaag agttcatttg ccacacgaat ggcttcgctc
     5641 agatttttct gtacaggctg gtggtcacag ccgttcatca tcaaccattg gttggtcgaa
     5701 gcgtaggcac gcacatctga caatttttgt ttccagaagg tcaaggcctc atctttgtca
     5761 actgggattt cattcccatt actgtaccag ttggcaaaga gaatacctaa aacacgacta
     5821 ccatccacac cctgccagta catttctgaa aactgggatg taaaccgctc atcttcaagg
     5881 acttggttgt caaatccaat cggcttcaca ccacgaccaa aggctgccac gtgaatacct
     5941 gatttttgaa ggatttgagg agcttgtccc atatttccaa aggtatctgg aaagtagcca
     6001 atctgggttg atttacccca tttggcagct tcttgttgac caatcaaggt attgcggaca
     6061 ttggcttcac tggagatcaa gtagtcatcc tgcaagatgt aaaagggacc aattttaagt
     6121 ttgccctcgt caatgtagcg ttggaccttg tcgcgatttt cagggcgaat ttgtaagtag
     6181 tcatcaagga caatagtttg tccatccaag tggaaactct tgaactcagg gtcattttca
     6241 aagagatcaa agagattgtc aaacaattcc accaactgca tacgatggct ttcaaaaggc
     6301 aagtaccact cacgatccca gtgactatgt gagataatat gtacaacaac attttccatg
     6361 aagtaaaacc tcattctaaa tttaaatttt aacgttttaa atgtaaactt gtgattctga
     6421 taagaatcgg ttgagctaaa gcgagcccct tagcggatat ccaagtaatc caagaccaac
     6481 tcacagaaca tcatgttagc ccaggagaac cattcacgag agtagagggt cggatcatct
     6541 acgtggaagc tttcgtgcat gacacctgta ccaccatcgc aggcaaccag ctgatccagc
     6601 aagaattttt tctctgcctt atctcttgtt gtcaatcctt ggatagaaag ggcgattggc
     6661 cagatatagc gatagaaggt atgagaactt ccgagaccgc tagcgtattc tccttggtag
     6721 aagtatggat tttcagagct cagaatggta cggcgagtgg cttgatacac ttcatcatcg
     6781 atcgaacagt agcccagata gggcgcagct agtagacttg gtacatttgg atcatccatg
     6841 atgctggcat ttcctaggcc atctacttca aaagcgtaaa tcttttcgcc cttgctgttg
     6901 gtggtgtaag cgtagttttc gattccttct tggatttcat cctgaagacg cttggcatca
     6961 gcaataacac tctggctatc agctaggttt aatgctgcga agatttcttg cacataaccc
     7021 aagactacta cagcaaacat atttgacgga atcaagtaac tatactggca acagtcatca
     7081 ctcggacgaa aagctgacca agtcatacct gtcactgcaa agtcaggtcc aaagccatca
     7141 tttaccaagg tgtcttcctt acggtccgta tctcggacaa aacgataagg agagttcttg
     7201 tggtcttgtt caactgtcca tagatggaga atttccttag tcgctgcgac aaaagtttca
     7261 tcaaactgac tagtctcgcc agtctctttc cagaggagat aagccaactg caaaggatag
     7321 caaagcgaat ccacctcata cttgcgctcc cagatccagc cgttaaggtc tgtgtggtca
     7381 gtctcgtggt gccctttcca gttctcctca atgttgaagg agttagcata gggatccttg
     7441 agtaccaagg tcatctgacg tttgaccaaa cctgcaatgg tctgacgcag gagggcatct
     7501 cttttagcta catgaaggta gggtctgagt tgggctgtcg aatctcgaag ccacatggca
     7561 ggaatatccc cagtcaagac aaaagttgaa ccatcttcta agatttcaac cgtattgtcc
     7621 aaggtatcgg tgtagcaacg ctcaaagaca tcaacccact ctggatggtc cttggcccgc
     7681 tctgctactt catctagcca ttctctaaca atttctttcg aataaatcat cgtgcaattc
     7741 cctctttcaa acaagtttca tgttcagtat atagcttttg agacagaaaa tacatacaca
     7801 aatgatagtc atttttctac aaaatagtta tctttaaaac tccttttcta aaggccctct
     7861 ttttctgata taatgtagaa aaacagctaa aggaaagact atgaaaccac tacttgaaac
     7921 catcgatacc cgctttggta ctgctagcaa gcatgccttc tctcggggaa ataccctgcc
     7981 atacacaggc gtgccttttg ggatgaatta ctttgtgccc cagaccagtg accaggatgg
     8041 atcttggttt tttgatccgc atctgcctat ctttcagggg attcgattaa ctcaccagcc
     8101 cagtccttgg attggggact actcttggct ccttctgaca cctgtcacca gtcaactggg
     8161 gggagacagc ctctttcacc gccagtcttc ttatgatata gataaagctt gcttccagcc
     8221 tcactatttg aagctctttt ctctgcgtta tcagattgaa acccagctca caccgacttg
     8281 ctatggtgct tctattcgtt tgaatcaaaa gcaaggcaaa gccctctccc tctatcttca
     8341 cgcagcagat gaactgacag tagagcaagt agataagcgg actcttgccc tgcgacaaga
     8401 aggtaaaact gaaactaaca aaaattcgct aacgatgttc actgccctgc aaatgaacac
     8461 ggatattctt gctatcagcc aagaagctgg ggactggcga attgacttag caagtagtca
     8521 aaccgagatg caactagcca cttctttcat ctctccttct caagctctaa tcaatctacc
     8581 tcaagaagat tttgatagct gtaaaacaag tgcccaagcg gattgggaaa atctcctcca
     8641 tcgttttgac attatagaga caggagaggc tgaccgaacc ttctttgacc actgcctcta
     8701 cagactcttc ctcttcccac agacttttta tgaggttgat gagtcaggga aagccatcca
     8761 catggatctg gctactggta ctgtcaagcc tggtgtcctc tttagcaaca atggtttctg
     8821 ggataccttc cgcaccacct tccccctctt tgcccttatc ataccggagc actatcaacg
     8881 ctttttagaa ggtttcctca atagctaccg cgatactggt ttccttccaa aatggctggc
     8941 tccagatgaa cggggtatga tgccaggtac actgctagat ggcattatcg cagatagcgc
     9001 ctgtaaggac atggcccccg acctagaagg agaactcttc caagccatgc tcgaaacagc
     9061 cagcaaagcc gaccctctcg gcatcaatgg ccgccacgga ctagcccaat accaagaact
     9121 aggttacctc tctaccgacc accacgaaag tgttagccat actctagact atgcctatag
     9181 tgacttttgt atcgccagct gtgccaaaaa actagagaac atagaaatcg ctgaaaccta
     9241 caaggctgct tcacaaaatt accgccagct atttgacgct gagacaggtt acatgcgagc
     9301 acgagacaat caaggaaact ttcaccctga cttctctcct tatagttggg ggcgagacta
     9361 cgctgaatgc tctgccattc aagctacttt aggcgttctc cacgacatcc ctggcttaat
     9421 ccaactgatg ggtggaaaag aaacctttag caactatctt ttgaaagcct gtcaagatgc
     9481 tcccctcttt gaaacaacag gctatggtta cgaaattcac gaaatgagcg agatggctac
     9541 tgctcctttt ggacaaatag ccatttccaa ccagcctagt ttccacattc cttatctctt
     9601 ccgctacagc gattaccctg actacactgc ccttcttatc aagacgctcc gtcagaaagc
     9661 ttttcaccca agttgggaag cctatcctgg agatgaagac aatggtagtc tctctgcttg
     9721 gtacatctgg tcagctctcg gattttatcc aacctgtcca ggaaaaccaa gctatgatct
     9781 cggaatccct ctctttgacc atctacgagt ctacctagct aaagaaaata aatggctaga
     9841 tattcatact gagcaaaacc acagccattt taactttgtc aaagaatgcc gactggacaa
     9901 aacactcgta tcaactattc aacaccaaga cctcctaaaa gctgaacaac taactttcac
     9961 cctcagctgg ctaccaaatc actaataacc aaaagaacct ttgcatcgcg caaaggttct
    10021 tcttttatga aacttggact tgaagttgat ccacaagctg accatccacc gttaggttca
    10081 gatagaaatc caaggtttta tctcctgcga aatacaaggt tggtagcgtc agcctttttt
    10141 cagtctcacc agcttgaaac gcaaggacat aaatctcgtc ctgataggcg acaccatgga
    10201 cacctgtccc cggttgaatc gaaatcttag cctctaaaga ggagctactt tcactacgtt
    10261 caatcctaaa atgaagagtc tctccctttg ccacagctag gcttttttct gcaaatgcta
    10321 gttcctgaac aacttctttt tttgataacc caggagtttt ataaagggaa atcttggtca
    10381 atacagggat agattgtgcc tcggtaatca tcacgcgcac cttttgtgcc tctactagcg
    10441 gacctcgcaa gaggcgctta tgaccaactg tgaagcccct gccaaactcc tgccagacac
    10501 catccacctc tacttgcaca tgaaaagcag cgattcgttg ccctagcttc aaatcttctc
    10561 ttaactcaat tacatcaaaa gttttaggag aacctaaatc gagttctaac tggatgggca
    10621 agtctgcatc gcttgcccaa gagctggtct caaggccgtc tgtcaaatgg cgacaatcaa
    10681 agtctgcgga aagagctgga ccagatacct cagctcccag agccaaatct tctttataga
    10741 gctcattgcg ataggtcgca aattcataaa gtcgttcaat atcctttgca tcaaagagcc
    10801 cagcttgatt cggcggaata ttaagcaaga gtggagttcc tcgccctact gagtgaaagt
    10861 agatttcgac caactcctcg agagacttag gatcctgatc ctcatggtag aaccagcctg
    10921 gacggatgga aacatctgcc tctccgattg aaaaaatcgt gcccgaggga tccccgtgct
    10981 gaagatagtt cagctctgct tctgttccta gtttatcaag attcaccttt tgccacagtg
    11041 gatcacctgc ataccctcgt tcattgccaa tccagcggat actggtgcct tctgttgaaa
    11101 aaatcaagca atcgccctgc aggtcacgaa tggtttcaaa ccatttttca aattcataat
    11161 taaccttttg cgcgccctct cctctggcac catccatcca aacctcagcg aacttaccag
    11221 cattcccata gttaggattt gataagattt ccttcaactg agccagataa taggcattgt
    11281 agtccgcttc tcggtccaca tgatagaggg gactatgggc atcccacggt gacaggtaga
    11341 cccccatatc catatcaaac tctgtggcag cttgggatac ttcaaggagc aagtcgccct
    11401 ttcctctcct ccaaggactg accttaaccg aataatctgt gtgagctgtc ggataaagga
    11461 caaagccatc gtggtgcttg accaccaaaa tcaacttttt gaagcccgtt tccttgagca
    11521 cacgaaccca ctcacgcgca tccaactgac tcgggttaaa gcgctcagga tcctcctgtc
    11581 cagtccccca ttcttggtca taaaaggtat taggaccgaa gtggataaaa gctgctagtt
    11641 catctcccag ataagctagc tgagtctgac ttggtaacgg tccatgcggt ttgattttct
    11701 tcatcgttat atatctcctt cttttgcaac ttctcttgtt cacaaggtta tccacaggtt
    11761 gtggataata aaccatgatc taacagccag ctctttccaa ctccaactgg taggctcagg
    11821 atttttctcc tcaatgttcc ctcttcaaac agctctgtca tatggttctg ctaacacctc
    11881 tcagaatgct actctccata attattctca ggcttctttc aggtcattgt gtgaagacca
    11941 ttcactctcc agacttttcc acagattgtg aataagtgat tattctaagc tatcacctcg
    12001 tcgactataa caaaaaataa aaagagggtc aataagataa ttatctcttc ctaaatactc
    12061 agaaaagatt ctataatttg tacaattgaa caagaaatgc ttctccaaaa tctatcttca
    12121 aaagaatgtt gcatcttttt atcctttcac acaaacattt ctctttatac agatccaagt
    12181 gataaccaga tgataattgg acactttttg gtcatttgta tattttttga aaaatttttt
    12241 aaaaaaattt tatatccttt ttatagaagt ttatgcattt gtatctatgt attctatcct
    12301 atgttatgta taataacata aaattgtgct aaacatcata atttatatta ttttccgaga
    12361 aattatagcc attgacatta cctaggaatt gcgttataac atacatgtaa gcggtaccca
    12421 aaatggtgcc aagtcaaaat ttttaaggag gaaaatacat gtcttctcat ccaattcagg
    12481 tcttctcaga aattgggaaa ctgaaaaaag ttatgttgca ccgtccaggc aaggagttag
    12541 aaaacttgtt gccggactat cttgaaaggc ttctttttga tgatattcct ttcttggaag
    12601 atgctcaaaa agaacatgat gcatttgccc aagctcttcg cgatgaagga attgaggttc
    12661 tctacctaga acaactcgct gctgaatcat tgacctctcc agaaatccgc gatcaattta
    12721 tcgaggaata cttagacgaa gccaacatcc gtgatcgtca aaccaaggtt gctattcgtg
    12781 aattacttca cggcatcaag gacaaccaag aattggttga aaaaacaatg gctgggattc
    12841 aaaaagttga attgccagaa attcctgacg aagctaaaga tctaactgac ttagttgaat
    12901 cagattatcc atttgcaatt gacccgatgc caaacctcta tttcactcgc gacccatttg
    12961 caacaattgg aaacgccgta tcgcttaacc acatgtttgc agatactcgt aaccgtgaaa
    13021 cactctacgg taagtatatc ttcaaatacc acccaatcta tggcggaaaa gtggatttgg
    13081 tctacaaccg tgaagaagat acgcgtatcg aaggtggaga cgagctaatt ctttctaaag
    13141 acgtccttgc agtaggtatc tctcaacgta cagacgcagc ttctatcgaa aaacttttgg
    13201 tcaacatctt caagaaaaat gttggcttca agaaagtttt ggcctttgaa tttgctaaca
    13261 accgtaaatt catgcacttg gatactgtct tcactatggt agactatgac aagttcacta
    13321 ttcacccaga aatcgaaggc aaccttcacg tttactcagt tacttacgaa aacgaaaaac
    13381 ttaaaattgt tgaagagaaa ggtgacttag ctgaacttct tgctcaaaac cttggtgtag
    13441 aaaaagttca tttgattcgt tgcggtggtg gcaatatcgt agcagctgcg cgtgaacaat
    13501 ggaacgacgg ttctaacact ttgaccatcg cacctggtgt ggtagttgtt tatgaccgca
    13561 ataccgtgac caataagatt ttggaagaat acgggcttcg cttgattaag attcgcggaa
    13621 gtgaattggt tcggggccgt ggtggacctc gttgtatgtc tatgccattt gaacgtgaag
    13681 aagtgtaatc gctgttcggt attcgtcaat agaaaatgta aaaaatagaa agaggaaata
    13741 ataaaatgac aaattcagta ttccaaggac gcagcttctt agcagaaaaa gactttaccc
    13801 gtgcagagtt agaatacctt attggtcttt cagctcactt gaaagatttg aaaaaacgca
    13861 atattcaaca ccactacctt gctggcaaga atatcgctct cctatttgaa aaaacatcta
    13921 ctcgtactcg tgcagccttt acaactgcgg ctatcgacct tggtgctcac ccagaatacc
    13981 tcggagcaaa tgatattcag ttgggtaaaa aagaatctac tgaagatact gctaaagtat
    14041 tgggacgtat gtttgacggg attgaattcc gcggattcag ccaacgtatg gttgaagaat
    14101 tggcagaatt ctcaggcgtt ccagtatgga acggtctaac tgacgaatgg cacccaactc
    14161 aaatgctcgc tgactacttg actgttcaag aaaacttcgg tcgcttggaa ggcttgacat
    14221 tggtatactg tggtgatgga cgtaacaacg ttgccaacag cttgctcgta acaggtgcta
    14281 tccttggtgt caatgttcac atcttctcac caaaagaact cttcccagaa aaagaaatcg
    14341 ttgaattggc agaaggattt gctaaagaaa gtggcgcaca tgttctcatc actgaagatg
    14401 ctgatgaagc agttaaagat gcagacgttc tttacacaga cgtttgggta tcaatgggtg
    14461 aagaagacaa attcgcagaa cgtgtagctc ttcttaaacc ttaccaagtc aatatggact
    14521 tagttaaaaa agcaggcaat gaaaacttga tcttcctaca ctgcttgcca gcattccacg
    14581 atactcacac tgtttatggt aaagacgttg ctgaaaaatt tggtgtagaa gaaatggaag
    14641 taacagacga agtcttccgc agcaagtacg ctcgccactt cgatcaagca gaaaaccgta
    14701 tgcacactat caaagctgtt atggctgcta cacttggtaa cctttatatt cctaaagtat
    14761 aattttagat aataaaccgt ctaccaacag ctatgagggc tgcgactaat agctttagtc
    14821 cggtcctctt ttatgtaatg gtaatctatt atttcttata aaatatgtga aaaatcatta
    14881 aattgaaatc taaacgcatt ctattgagtg tgataaagga gaatttatgg caaatcgtaa
    14941 aattgtagta gctttgggag gaaatgcgat tctttcttct gacccatcag caaaggctca
    15001 acaagaagct ttagttgaaa cagctaagca tcttgtaaaa ttgattaaaa atggagatga
    15061 tttgattatc actcacggta atggacctca agttgggaat ctcttgctcc aacatttggc
    15121 atcagactct gaaaagaacc ctgccttccc actcgactca cttgtcgcta tgacagaagg
    15181 tagcatcggt ttctggttga aaaatgcttt gcaaaatgct ctcttggatg aaggcatcga
    15241 aaaaaatgtt gcctctgttg taacgcaagt tatcgtagat aaaaatgatc cagcttttgt
    15301 taacttgagt aaaccaatcg gtcctttcta ttcagaagaa gaagcaaaag cagaagccga
    15361 aaagagcgga gcgactttca aggaagatgc tggccgtggc tggcgtaagg tcgttgcctc
    15421 accaaaacct gttgacatca aagaaattga aaccatccgt actcttttaa ataatggtca
    15481 agtcgtcgta gctgcaggtg gtggcggtat tcccgtcgtc aaagaaaaca atggacattt
    15541 gactggtgtc gaagcggtta ttgataaaga cttcgcttcc caacgtttgg cagaattggt
    15601 tgatgcagac ctcttcatcg ttttgacagg tgtagattat gtatttgtta actacaacaa
    15661 gccaaaccag gaaaaattgg aacatgtgaa tgttgcccag ctggaagaat atatcaaaca
    15721 agatcagttt gcaccaggta gcatgcttcc aaaagtagaa gcagctatcg cttttgtcaa
    15781 tggtcgtcca gaaggaaaag cagttattac ttcccttgaa aatctaggcg ccttgattga
    15841 atctgaaagc ggaacaatta ttgaaaaagg ataagttgtt ttactaataa gatgtattct
    15901 atttctagta tctttatatc aaattagaaa ttattcttga aaacatgtac aatatttcaa
    15961 aagatactag ttttagactt taatatggta aaacaaatat aaatagaaag cgttttcttg
    16021 aatgtttatt taagaaagta gttggttttt tacactttgt tagacatcag gaggaaaaac
    16081 aaatgagtga aaaagctaaa aaagggttta agatgccttc atcttacacc gtattattga
    16141 taatcattgc tattatggcc gtgctaactt ggtttatccc tgcgggggcc tttatagaag
    16201 gtatttacga ggctcagcct caaaatccac aagggatttg ggatgtcctc atggcaccga
    16261 ttcgggctat gctaggtact catccagagg aaggttcgct cattaaagaa acgagcgcag
    16321 cgattgatgt agccttcttc atccttatgg ttggtggttt ccttggcatt gtcaacaaaa
    16381 ctggtgctct tgacgtaggg attgcctcta tcgtgaagaa gtataagggc cgcgaaaaaa
    16441 tgttaatttt ggtattgatg cctttgtttg ccctcggtgg tacaacttat ggtatgggtg
    16501 aagaaacaat ggccttctat ccactccttg tgccagttat gatggccgtt ggttttgata
    16561 gcctgactgg tgttgcaatt attttgctcg gttctcaaat cggctgtttg gcatctactc
    16621 tgaatccatt tgcgacaggt attgcttcag cgactgcggg agttggtaca ggggacggta
    16681 tcgtacttcg tctgatcttc tgggttacct tgactgctct tagtacttgg tttgtttacc
    16741 gttatgcgga taagattcaa aaagatccga ctaagtcact ggtttatagt actcgcaaag
    16801 aagatttgaa acactttaac gtagaagaat cttcatctgt agaatctaca cttagcagca
    16861 aacaaaaatc agttctcttc ttatttgtgt tgacattcat cttgatggta ttgagcttca
    16921 ttccatggac agaccttggc gttaccattt ttgatgactt taatacttgg ttgactggtc
    16981 ttccagttat tggtaatatt gtcggttcat ctacttctgc actaggtact tggtacttcc
    17041 cagaaggcgc aatgctcttt gcctttatgg gtatcctgat tggtgttatt tatggtctta
    17101 aagaagataa gattatctct tccttcatga atggtgctgc tgacttgctc agtgttgcct
    17161 tgatcgtagc gattgctcgt ggtattcaag ttatcatgaa cgacggtatg attaccgata
    17221 caatcctcaa ctggggtaaa gaaggcttga gcggtctatc ttcacaagtc tttatcgttg
    17281 taacttatat cttctatcta cctatgtcat tcttgatccc atcttcatct ggtcttgcca
    17341 gcgcaactat gggtatcatg gctccacttg gagaatttgt aaatgtccgt cctagcttga
    17401 ttatcactgc ttaccaatct gcttcaggtg tcttgaactt gattgcacca acatctggta
    17461 ttgtgatggg agctcttgca cttggacgta tcaacattgg tacttggtgg aaattcatgg
    17521 gcaaactcgt agtcgctatt attgtagtga ccatcgccct tcttctcctt ggaaccttcc
    17581 ttccattcct ataaaatagt gagtgaggtg attccatgaa aatagatata acaaatcaag
    17641 ttaaagatga atttcttata tcattaaaaa ccttgatttc ctatccttca gtactcaatg
    17701 aaggagaaaa tggaacacct tttggacaag caatccaaga tgtcctagaa aaaactttag
    17761 agatttgtcg agacataggt ttcactacct atcttgaccc taaaggttat tacggatatg
    17821 cagaaatcgg tcagggagca gagcttctgg ccattctctg tcatttggat gttgttccat
    17881 caggtgatga agcagattgg cagacaccgc catttgaagc aactatcaaa gacggctggg
    17941 tattcggacg tggtgtccaa gatgataaag gcccttcgct cgcagctctc tatgcagtaa
    18001 aaagcttgct ggaccaaggt attcagttca aaaagcgcgt acgctttatc tttggtaccg
    18061 atgaggaaac cctctggcgc tgcatggcac gctacaatac catcgaagaa caggccagta
    18121 tgggctttgc acctgactca tcttttcctc tgacctatgc tgaaaaaggg cttctacagg
    18181 tcaaacttca tggccctgga tcggatcaac tagagcttga agtaggaggc gcctttaacg
    18241 ttgtaccaga caaggccaac taccaaggtc ccctctatga acaggtttgt aacggtctca
    18301 aagaagctgg ttatgattac caatccactg aacaaaccgt aacggttctc ggagtgccaa
    18361 agcatgctaa ggatgctagt caaggtatca atgctgtcat ccgactagct accattcttg
    18421 ctcctctcca agaacaccct gctctcagtt ttcttgcaac acaagcaggt caagacggca
    18481 caggaagaca aatctttggt gatatagcag atgaaccttc tggtcaccta tcctttaatg
    18541 tcgcaggtct catgatcaat catgaacgtt ctgaaatccg tattgacatt cggactcctg
    18601 tcttagctga caaggaagaa ctagtagagt tgcttacaag atgtgcacaa aactaccaac
    18661 tccgctacga agagtttgac tatctagcgc ctctatacgt cgcagaagac agtaaactcg
    18721 ttagcacact gatgcaaatc taccaagaaa agactggcga taacagtcct gctatttcat
    18781 ccggtggtgc cacttttgct cgcaccatgc caaattgtgt agccttcggc gccttattcc
    18841 caggagcgaa gcagacagaa catcaggcaa atgaatgtgc cgttctagaa gatttgtacc
    18901 gtgctatgga tatttatgcc gaagccgtct atcgacttgc aacttaatca ggcaactgtt
    18961 tctaccaaaa aaaatcgacc gattaatgaa ctgcacccca aaagttagac agaataaatc
    19021 taacttttgg ggtgttttat tatgaaattg agttatgaag ataaagttca gatctatgaa
    19081 ctaagaaagc aaggacaaag cttcaaacag ctttcaaaaa gatttggtgt ggatgtttct
    19141 ggtctaaagt catctgaatc tttgagatga gctttataaa tcgctttttt cagtttttgc
    19201 actggtgttt cgataaactc aaacttttta gccgtggtat tgcctgattt tatagtatat
    19261 tgaaactaga atagtacacc tctacttcta aaacattttt agaaatcgat ttgactgtcc
    19321 tgaacgattt gtcctgttct tatttcattt tactatattt gagccacttc gtctttaacg
    19381 gctttattca taagctcttg taatttttct ttactatcaa ttacttctga ttttccgttg
    19441 taatttattg taataggttt taacttacct aatttctcga cacgctcatt aatttgatct
    19501 tttttgaagg ctgcatatgt ttttcctaag attttttcga aaatatattt atcagatagc
    19561 ggtttgcctt cttcttcagc ttggtttttg tattgatttg aaacataagg aacaaatcct
    19621 tcatagtaac ctaatgctcc cataagttca aaagcttgtt ttctaattca aaccattgca
    19681 actcagattt cagcttttca gataaatcct gctcatccaa ataatgactt gaaattagtg
    19741 ctgaactcgt ttctgtatcc tgtacaggct gatcacccat accagcaaaa aataaactcg
    19801 ttcctagcaa gaccgaacaa gctcctattg catatttcct caaagaaaaa cgctgctttc
    19861 tctcaaattg aaattctttc atcccatctc ccatcattca ttattactgt atattttgta
    19921 tatcagaaat agtttgtatt cacaaatctt tctagttatt cccttatcat tcctaattaa
    19981 gggaggtaac atacaataat ttttagttaa atgtatatct atgttttttg tttttcttaa
    20041 taaacgcaat acaaaaagag cctgttgcca agctctttgt actcaatgaa aatcaaatag
    20101 caaactagga aactagccac aggttgctca aaacaccgtt ttgaggtagt agataagact
    20161 gacgaagtca gctcaaaaca ctgttttgag gttgcagata gaactgacaa agtcagtaac
    20221 atctatacgg caaggcgacg ctgacgtggt ttgaagagat tttcgaagag tattagtcta
    20281 ttatttcttc tcagcgcgaa gggctgacaa gatttgtgtt cggatatcat ccacaccatt
    20341 tggcgtattt ggtaaaaaga tggtttgatt tcctttagag gcaaaggtat tcaaggtatc
    20401 caaatactgg ttggtcaaga ggatagacat gatttgttct tctgtcatgc caacattggc
    20461 ttccttgagt tcggtgatag actctgccaa tccatccaca atcgccttac gttgttgggc
    20521 aatccccaca ccatgaaggc ggtctttttc tgcttctgct tcagctgcag tgacaatttt
    20581 aatcttgtca gcttccgcca attcttgtgc tgcgacccgc ttacgttgcg ccgcattgat
    20641 ttcattcatg gattgcttaa cttctgcatc cggttcgact ttggtaatca aggttttcac
    20701 gataatgtag ccgtaagtgg tcatttcttc tgctacttgg tgttgaactt caagggcaat
    20761 ctcatctttt ttctcaaaca attcatccaa ggttaatttt ggaacagaag agcgaagagc
    20821 atcttcgata taagatttaa tctgagattc tggacgtatg agtttatagt aagcatctgt
    20881 cacgctctgc tcgttgacac ggtactgagt cgctacattc atcataacga acacattgtc
    20941 cttggtctta gtctcaacca caatatcact ttgcaacaag cgcaactgaa tccgtgctgc
    21001 aatcgagtca atcccaaaag gcaagcgaat atgaataccg ctattagcaa ccttttggta
    21061 tttcccaaag cgttcaataa tcgccaccga ctgctgacga accacataaa ctgtactcag
    21121 tgtgactatc accaatagga gcacacaaac aatcagaaaa atcatgaaaa atattgccat
    21181 aatggaacct ccacaagtat ttttctagta ttatagcaca tttaaagaag gctgtgccgt
    21241 ttttactgcg atttttcctg aaatgtcaat aattagaggt gaattgtcct attgtcgtcc
    21301 aatctcttgc taaaataact ctttataaaa ggcaatcgtt tcttctaagg ttggcataaa
    21361 tggatttcct ggtgcgcagg catcaatcaa ggcattctta gaaaggtatt caaagtcgaa
    21421 atctttttct tcaataccaa gttcagtcag tttcttagga atacctactg tctcagaaag
    21481 cttctcaatc tcaacaatcg cataatcggc acattcttga tctgatttac cttctacatg
    21541 aagtcccaag gctttggcaa cattgcggaa agcttctggt acacgtttag cattttcatg
    21601 ttctataact ggtagcaaca tggcacagca aacaccatgt ggcaaattat atactgcacc
    21661 gagttggtga gccattgagt gaacataacc aagaccagca ttattaaagc tcataccacc
    21721 aaggaagatg gcattgacca taccttcacg cgcttcaata tcatgtccat tttctacagc
    21781 acgaggaaga tattccttga taagctcaat agcaccaata gacagtttct tggttacatc
    21841 ataagcacca ggtgttacca aagcttcaac agcgtgagtc agagcatcca taccagtagc
    21901 agcagtcaag gcttgaggtt tggaaagcat gagttcaggg tcattgacag aaatcaaggc
    21961 aaggctattc ttgtcaacca ttaccatctt aaccttgcgt tcttcatcag taatcacata
    22021 gttaatagtg atttctgctg atgtacctgc cgttgtattg atagccacaa ccggcaagcc
    22081 ttttttagca gacttatgaa gcccttcata atcctgtggt tttccaccat ttgtagccat
    22141 gatagaaata caactagctg catcctgtgg agaacccccg ccaagactga tgataaaatc
    22201 acatctatgt tcttgcaaag cagctacccc atctgtgaca tttttacaag tagggtttgg
    22261 ctctacatcg ctaaagatga catattcgat tccttctgta tccagtggtt ttaaagtctt
    22321 aggtaaaata tcactgcctt cgatgtactt atctgtcacc aaaagtgcct ttttatagcc
    22381 aagttccttg atataaggac ccacttcatt tacaacacct tttccaataa ggttgactgc
    22441 cggaacataa aatatagcca tataattttc ctcctgcgcc tccgcgctat ctatttaaca
    22501 tcattatatt actttttgta ttcgttttca attatttgtg aattgtttaa caaatctttt
    22561 taaaaggata ggagggacgt aagttcacct cctatataaa caagttttat ttatgtagtg
    22621 gtcccaacaa ctgacatgca cgatagtctg ctgattctag atcttctgtt ccaaataagg
    22681 accaattttt aggtctaaag atatcttcct caggtacatt atgcatattg acaggaattc
    22741 tcaacataga agccaaggta atcaagtctg ctccaatgtg tccatatgtt atggctccgt
    22801 gattagctcc ccaattattc atgacgtcat agacagactt gaaagcacct tttcctgtca
    22861 aacgtggagc aaaccaagta gttggccatc ctgaatctgt acgattatct aaagtatggt
    22921 gaacatcttc aggaagttca agtgtgtaac cttctgcaat ttgtagcact ggaccaaccc
    22981 cttttagaag attgagacgt accattgtta ctggcatatc ccccttcgtc aagaaacgag
    23041 ttgagaatcc tcctccacgg aagtactcgc ggtttgctgg tgggaagtct gtattttcaa
    23101 gcatagcctg tacttcactt tcttccaact cccagaatgg tttcataaca ggtttgccat
    23161 ctcgagtagc ttgacctgta ccatccaatg tacaagaacc agagttgatt agatgtaaga
    23221 agccagctgc agcacaaccc tctaaagtat gtcccgttac acgtttaaca gcctctggac
    23281 tccaataagt gcgcacatca gcaaagattt gtggagtatt tgttaataga taattaaaga
    23341 gcatagacac accatttagt gaatcattct ctgtcgcaaa tacaaatggt tttcgaatac
    23401 cattccagtc aaactgagta ttgaggaaag tttccataaa atctccattt ggaaaatggt
    23461 ctgtccactg acgttgacct tggaaaccag ctactaaagc atggtgacca accgcttctt
    23521 cctcaaaacc aagttcagca agccttgggt taccaaccat taagtcacgt ccaatcatga
    23581 acatcttaat aacaaattcc cattgttttt ctttctcttc tttagaaaga atcaaatcct
    23641 cacgatttcg gtcaacccct tctttgatat gttcttttac ccaaaccata gcacgttcga
    23701 actcttctgg gtcataaata ccacggtcca tacggcgcgt gaactccgtc atatctaccg
    23761 attcatttcg cattcctaag tattcttgga agaaatctgg atttacaata gaaccgccaa
    23821 tccccatcga aacactaccc attgataggt aagcagtgtc tctcatcaag ccagttgcaa
    23881 gagctgcacg cgcataacgt aaaagttttt ctttgacatc ttctggaata tctgtatcat
    23941 tagcttcctg aacatctcta ccataaatcc caaaggctgg aatccctttt tgagcatgtg
    24001 aagctagtac agctgcaaga tagacagctc ctgggcgttc tgtcccatta aatccccaaa
    24061 tagcatgagg aatatctgga gacatatcca tagtttcact accataacac cagcatggtg
    24121 taactgtaat tgttgcgcaa acatttgatt ttttaaacaa ctcatgggaa gctgcagcct
    24181 ctggaacacg accaatggta gatggagaaa tcacacattc cacaggttcc ccatctggat
    24241 atttcaatgt gcttgaaatc aaatctgcca cacttttagc catgttcatt gtttgtactt
    24301 caagtgattc gcgtacacct tgacgacgac catcaatagt cggacgaatc ccaatacgtg
    24361 gatgttgaat catactattt tcctcttatt tttctgtttc tttcatacta ccgatgataa
    24421 tttttaaaat tacttataag tataaacctc tacctctgcc aaacttaaag gaattcctgc
    24481 gtttctcaaa gaaattctaa ccttttttcc cttcagccct tttaagtcta tggatagatt
    24541 attgctttct aaactgtcaa tatgttgttt aaaaacttca tgattatttg aatcatatat
    24601 aataatatca aaatttgata atctctgaat ttcagcatct gttctattat aaataattaa
    24661 cttttctaat tcttcggttt gtgctaaatc tatctcccac catgaaggag aatctccttt
    24721 ggtatgagtc actgaatgat gaccataatc tccgttttta tttccatcaa ctgctagacg
    24781 agaaaaacca ttgtaatccg ttgaactctg tcgagtttct ttggttaatg caatattaga
    24841 aagtttcttc ggagttctct ttgaaccata ggcttcaact tccgctaaac ttaacggaat
    24901 agctgctgat tttaattcta ttttgactag cttagcccct acagaaggta cttttaaaga
    24961 taacaaacca tcaactactt tatcaaaatg tcttctaaaa acttcttctc ctgatgaaga
    25021 tagaaaaata acatcaaaat tagataaacg ctgaggttcg gcatctgttc tattataaat
    25081 atcaacttta gaaaccgtaa actcttctcc cagatcgacc tgccaccaag cgttagaatc
    25141 tgccttagta tgagtcaccg aatgatgtcc gtaatctcca tctttattac catcaactgc
    25201 taaagcagct acaccctcat aattggttga actttgagta gctaccttat ttgtagagac
    25261 tactttatct tctgttattt tatctatatc ctcttcagat tgcttaccat ctgattctct
    25321 aaaaacctct acttctgcta aactcaaagg cactccactc gttagtaatt taactttaat
    25381 ataccttgct cctttttctt tgaaatctag actaacagat tcacccgaca aattattaac
    25441 atgtttctta gcaacttcgt ttctattatt gtcatataga atcacatcaa aattagatag
    25501 acgttgagtc tcagcatctg tgcgattata aattttaaca agcccaactt tatccatttt
    25561 tttcaaatcg acttcccacc aagagggatt atctgcccta gtgtgtgtta ccgaaccaga
    25621 gttaaaatta ccatttctgt taccatccac agctctatta gggtctccat tgtaatctac
    25681 agaactttgt gttgttggtt ttgcatatgc tatattcaaa ttaccatcat taaatttatc
    25741 tggtgttggg aaagggactg ggcctgtccc ttcagtattt atagacatct ctaccattcc
    25801 attatgatta accgtaatgg tataaacatg ggtattctca tcccaatttt ctgtataagt
    25861 ataatgattt ttatcgccac ttatatttat ctgaggttta tgaccagtat gcccttttaa
    25921 tgtaagtgtt gttgtcctaa agtcattatc tacaggattg atggaataat ttttgcttat
    25981 ccagttcgct aactctaatt cttctttctt ccaacttttt gatgcatcaa aatttcctga
    26041 taatgcccac atagctgtct tatctgtcct gtaatt
//
LOCUS       DAVBCB010000028        25336 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVBCB010000028 DAVBCB010000000
VERSION     DAVBCB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25336
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.028"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..309)
                     /locus_tag="VQU28_001125"
     CDS             complement(<1..309)
                     /locus_tag="VQU28_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HET2476486.1"
                     /translation="MKKKWMYYAACSSNESADDSSSDKGDGGSLVVYSPNSEGLIGAT
                     IPAFEEKYGIKIELIQAGTGELFKKLESEKEVPVADIIFGGSYTQYATHGELFENYIS
                     K"
     gene            complement(897..1673)
                     /locus_tag="VQU28_001126"
     CDS             complement(897..1673)
                     /locus_tag="VQU28_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HET2476487.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            1795..2541
                     /locus_tag="VQU28_001127"
     CDS             1795..2541
                     /locus_tag="VQU28_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2476488.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            2554..3534
                     /locus_tag="VQU28_001128"
     CDS             2554..3534
                     /locus_tag="VQU28_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HET2476489.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            3729..4049
                     /locus_tag="VQU28_001129"
     CDS             3729..4049
                     /locus_tag="VQU28_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2476490.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            4095..4403
                     /locus_tag="VQU28_001130"
     CDS             4095..4403
                     /locus_tag="VQU28_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2476491.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            4420..5718
                     /locus_tag="VQU28_001131"
     CDS             4420..5718
                     /locus_tag="VQU28_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2476492.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASANLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQTLGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVITQLVILAMSTLVYFPFFKVQDRLAYQNEIKQS"
     gene            5869..8307
                     /locus_tag="VQU28_001132"
     CDS             5869..8307
                     /locus_tag="VQU28_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HET2476493.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMTIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            8326..8994
                     /locus_tag="VQU28_001133"
     CDS             8326..8994
                     /locus_tag="VQU28_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HET2476494.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVTQN
                     SRSI"
     gene            9012..10100
                     /locus_tag="VQU28_001134"
     CDS             9012..10100
                     /locus_tag="VQU28_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HET2476495.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            10555..13056
                     /locus_tag="VQU28_001135"
     CDS             10555..13056
                     /locus_tag="VQU28_001135"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HET2476496.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSTEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEIPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPESELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLIRSKEIVAENNGALD
                     KVYNETVKAVTEQIDSLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAATGESISYVTWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVISVPNKLVNIVVK"
     gene            13250..13945
                     /locus_tag="VQU28_001136"
     CDS             13250..13945
                     /locus_tag="VQU28_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476497.1"
                     /translation="MPVNEYGQMIGESMEGYTPGELPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPQEMWTYLFQEPVADMGELVSLLHQMLARKDRFYYAIIDKATGKALG
                     SFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYVFEELNYRRYEWKCDAL
                     NLPSRRAAERLSFIYEGTFRQAVVYKGRTRDTDWLSMIDKDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            13957..14373
                     /locus_tag="VQU28_001137"
     CDS             13957..14373
                     /locus_tag="VQU28_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476498.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEQALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVLVQDLIVLPIYQRQGIGSALMKEALEDYKDAYQVQ
                     LVTEETERTLGFYRSMGFEILSTYNCIGMTWMNRKK"
     gene            complement(15000..15092)
                     /locus_tag="VQU28_001138"
     CDS             complement(15000..15092)
                     /locus_tag="VQU28_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HET2476499.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            15253..16224
                     /gene="ruvB"
                     /locus_tag="VQU28_001139"
     CDS             15253..16224
                     /gene="ruvB"
                     /locus_tag="VQU28_001139"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HET2476500.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            16205..16777
                     /locus_tag="VQU28_001140"
     CDS             16205..16777
                     /locus_tag="VQU28_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HET2476501.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            17008..17766
                     /locus_tag="VQU28_001141"
     CDS             17008..17766
                     /locus_tag="VQU28_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HET2476502.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            17775..18578
                     /locus_tag="VQU28_001142"
     CDS             17775..18578
                     /locus_tag="VQU28_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HET2476503.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            18600..19859
                     /gene="rseP"
                     /locus_tag="VQU28_001143"
     CDS             18600..19859
                     /gene="rseP"
                     /locus_tag="VQU28_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HET2476504.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            19872..21725
                     /locus_tag="VQU28_001144"
     CDS             19872..21725
                     /locus_tag="VQU28_001144"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HET2476505.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            21825..23204
                     /gene="bglA"
                     /locus_tag="VQU28_001145"
     CDS             21825..23204
                     /gene="bglA"
                     /locus_tag="VQU28_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HET2476506.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTVFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNELIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIHDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            23396..25204
                     /gene="glmS"
                     /locus_tag="VQU28_001146"
     CDS             23396..25204
                     /gene="glmS"
                     /locus_tag="VQU28_001146"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HET2476507.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKKALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
BASE COUNT         7736 a         4313 c         5536 g         7751 t
ORIGIN      
        1 ttttgaaata tagttttcaa agagttctcc gtgggtagca tattgtgtat aagaaccacc
       61 aaagataata tcagctacag gaacttcttt ttctgactct agttttttga aaagttctcc
      121 agtaccagct tgaatcagtt ctattttgat accatatttt tcttcaaagg caggaatagt
      181 tgctccaatt aagccttctg agtttggtga ataaacgact agcgaaccac cgtctccttt
      241 atcagatgaa ctgtcatcgg cagattcatt agaagaacaa gcagcataat acatccattt
      301 ctttttcatg atggatacct ccgttgtgtt atttaagttt attttaaaac aatgtaagcg
      361 tttttaaaac ctacaattct attctatagt gtattgaatc tataacagta cacattgact
      421 gctaaaatat ttctataaat taatttgact ttcctgatag agatgttcac atcttatttc
      481 aattcactat attagagtaa aattctctac aaaaaaaaag aatagcctat tttactattt
      541 ttccgagtga tttcaattcc tttggggaaa tatggagata ctttttaaat cctgacaaat
      601 ggttgtttct ttttctaaat cggtgatact gtatcggaga atgcgcgtga ggtcacaaag
      661 gctgcgatag agcttctatg gagaatttct ttttggagag attttttaaa ggaatgagac
      721 atccgctacc tccttggaag gtttttttgt gataagtata gcagtttaaa aattctagag
      781 caaggtaaaa tagaaaaggt ttggctaaaa ttttttagct aaaccttttg aaaagttaag
      841 tatgtattat agatgcttgt tatctgcaaa tagctagagc atttcaagga aataaactag
      901 aaataacaat taatatggtg gttcagaaat accttttgat aatcaataag atcttcagga
      961 tgaagtgcgt tgattccatc catttcatat ttccgattta ataaacgata tttgttttca
     1021 ccaaattgat gaaaagggag tagttgaact tggtcgatat ttaatgagtt aaatagagta
     1081 gcgaattttt ctgcatcctc taaactattg ttaaaattag gaataactgg gattcttaaa
     1141 acgatagttt tattttgtga aaaagcataa tgaatgtttt taataatcat ttgattaaaa
     1201 accccagtca cttttttatg ttttatagaa ttataatgtt ttaggtctgt gtagataaaa
     1261 tccacatatt gaattaaatc aataaatttt tcatgatcaa caaaggcagt agtttcaatg
     1321 gcagtgtgta tgtgatgttc tttagctgat tttaagatag ctttagcaaa ttcaaactga
     1381 gcaaatattt cacctcctga taaagttaaa cctccaccgg attcttcgta aaattctttg
     1441 tcttttaata cctctgtaat aatttcttct acagtctttt cttcaccgac taaggtgaat
     1501 ttctctcgtt gagcatcttt cattttttca ggtttcattc tttgagattc aggattagaa
     1561 caccatggac agcgcagagg acatcctttt aaaaaaacag ttgtacgaat acccgggccg
     1621 tcatgaattg aaaagtgttg aatattaaaa ataattcctt tagatatttc cataagagtt
     1681 cctcctcttc ataagtctat cctatcacaa aaacgaaaga aaaacaatta cgaacgaaag
     1741 gttaatttgt tgttttttgc tttttatttt ggtagaataa aacggaggtg taaaatgaaa
     1801 agattggaac aaattattaa attagtatca gaatatgaaa agatcgacgt taatacatta
     1861 tcggaaaaat taaatgtatc gaaagtaacg attagaaaag atttagataa attagagtca
     1921 aaaggtttat tacacagaga gcatggatat gctgtattaa atagtggaga tgacttaaat
     1981 gtacgtttgt caattaatta tgaaattaag agaaaaattg ttcaggaagc agtaaaattg
     2041 gtgtcagata atgaaacgat aatgatagaa tctggatcga cctgtgcttt acttgctgag
     2101 gaaatttgca agcaaaaaag aaatgttacg attgtaacaa attcattttt tatagcaaat
     2161 tttgtgagag cttatgattc atgtcgtatt attgttcttg gtggagagtt tcagaaggat
     2221 tcacaggtta ctgtgggacc tttattaaaa gaaatgatac agacttttca tgtgcatcaa
     2281 gcttttgttg ggacagatgg atacgataaa gagatgggct ttacaggaaa agatttaatg
     2341 cgcagtgagg tagttcaata tatttcagca gcgtcggata aagtcattgt cctaactgac
     2401 tcaagtaaat ttgataaaag gggtacagta agaagatttg ctttaagtca agtctatgaa
     2461 gtaataacag acgaaaaact ttctaaacaa aatatagcta cattagaaaa tgctgggata
     2521 atggttaagg tagtttcgta agaggttaag tgtatgaatc aagataggaa taaactgctc
     2581 tctaaaattg cttatctgta ttatattgaa aacttaaatc agtcacaaat agcagcaaaa
     2641 ttaggaattt atagaacctc tattagtaga atgttaacag aagcaaggaa tgcaggaatt
     2701 gttaaaattg aaatagagaa ttttgatacc aatatgttta agttggaaaa ttatgtaaaa
     2761 gaaaaatatg gtctggaaag tttagaaatt attccaaatg aatttgatga taatccaaca
     2821 attttatctg aaagaatttc tcaagttgca gcaggtgtcc ttaggaatct aattgatgat
     2881 aatatgaaaa ttggcttttc ttgggggaaa agtttaagta atttagtaga tttaattcac
     2941 agtaaaagtg tccgaaatgt tcacttctat cctctagcag gtggtcctag tcacatacac
     3001 gctaaatacc atgtgaatac actgatttat gaaatgtcta gaaaatttca tggagagtgt
     3061 acatttatga atgcaacgat tgtgcaagaa aataaattgt tagcagatgg tattttgcaa
     3121 tcaagatatt ttgaaaattt gaaaaatagt tggaaagatt tagatatagc tgtagtcgga
     3181 attggtgatt ttagcaataa aggaaagcat caatggttag acatgcttac agaggatgac
     3241 tttaaagaat tgaccaaagt taaaactgta ggagaaattt gttgtcgatt ttttgattca
     3301 aaaggtaaag aagtctatga aaacttacag gaaagaacga tagcaatctc tttggaagat
     3361 ttaaaaaata ttcctcaaag tttagctgtt gcttacggtg atacgaaagt atcttcgatt
     3421 ctttctgtct tgcgtgctaa tttagtaaat catttgatta cagacaaaaa tacaatttta
     3481 aaagttttgg aagaagatgg ggatttgact tttagagaga ttctaggtga gtgaaaatga
     3541 tagactgatt cagtttatcg tttttctttt tagttgattg cacatttgtg cttatataaa
     3601 caaaaatagt ttatctgttg tttttggatt gacaacttta ttatgtagtt gtattctata
     3661 gttacaaaag aaaattttaa aatttcaaat gaaaaaagct ttttacatag tgaaatgagg
     3721 aggaatttat ggaaattatt gttccagatc aaattatcat gggtttaatt ttatatgctg
     3781 gtgatgcgaa acaacatatt tataaagcgt tagattacat aaaaaatggt acatgtgaac
     3841 ggtgtgaaga agaaatccag ttagctgatg ccgccttatt agaagcccat aatctacaaa
     3901 caaaattctt ggcacaagaa gcgtctggta caaagacaga aattacagct ctctttgttc
     3961 attcacaaga tcatctcatg actagtatga cggagattaa tttaatcaaa gaaattatta
     4021 gtttgagaaa agaacttcat aaaaaataat actagagtat tatcattgtt attaacatag
     4081 aggaggaaaa cataatggtg aagattggtt tgttttgtgc agcaggtttt tctactggta
     4141 tgcttgtaaa taatatgaaa attgcagcgc aatctagtgg agttgaggca gaaatagagg
     4201 cgttttctca gtctaaatta gcggattatg cgccaaatat agatgttgca ctattgggtc
     4261 cacaagttgc ttatacatta gataaatcaa aagaaatttg tgataagtgt gatgttccga
     4321 tagctgttat tccgatgatg gactatggta tgttagatgg gaaaaaagta ttagatttgg
     4381 ccctatcttt gattagtggg taagaaaagg agatttatta tgtcaaagat ggatgttcag
     4441 aaaatcattg caccgatgat gaagtttgtg aatatgcgtg gcattatagc tctaaaagat
     4501 gggatgttag caattttgcc attgacagta gttggtagtt tgttcttgat tatgggacaa
     4561 ttgccgttcg aaggattaaa taagagcatt gctagtgttt ttggagctaa ttggacagag
     4621 ccgtttatgc aagtatattc aggaactttt gctattatgg gtctaatttc ttgtttttca
     4681 attgcctatt cttatgctaa gaatagcgga gtagaggctt taccagctgg agttctatct
     4741 gtatctgcat tctttatttt gctaagatca tcttatatcc ctaaacaagg tgaggcgatt
     4801 ggggacgcta ttagtaaagt ttggtttgga ggccaaggaa ttatcggtgc tatcattata
     4861 ggtttggtag taggaagtat ttataccttc tttataaaga gaaaaattgt tattaagatg
     4921 ccagaacaag ttccacaagc tattgccaaa cagtttgaag caatgattcc agcatttgta
     4981 attttcttat cttctatgat tgtatatatt ttagcgaagt cattgactaa tggcggaaca
     5041 ttcatagaaa tgatttattc tgctattcaa gttccgttgc aaggtttaac tggatctttg
     5101 tatggtgcta ttggaattgc attctttata tcatttttgt ggtggtttgg tgttcatggg
     5161 caatcggtag taaatggagt agtgacagct ctgcttttat ctaatcttga cgctaataaa
     5221 gctatgttag cctctgctaa tctatcatta gaaaatggtg cacatattgt tactcaacaa
     5281 tttttagatt catttttaat tctatcaggt tcagggatta cgtttggtct tgtagttgcc
     5341 atgctttttg cagcaaaatc aaaacaatac caaaccttag gaaaagttgc agcttttcca
     5401 gcaatattta acgtaaatga gccagttgta tttggatttc cgattgtcat gaatccagtt
     5461 atgtttgtac ctttcattct tgttcctgta cttgcagctg tgatagtata tggagctatt
     5521 gcaacaggtt tcatgcagcc attctcaggg gtaacattgc cttggagtac accagctatt
     5581 ttatcaggat ttttggtggg tggatggcaa ggagttatta ctcagctggt gatattagcg
     5641 atgtctacat tggtttattt tccattcttt aaagtacagg atcgtttagc ttaccaaaat
     5701 gaaatcaaac aatcttagag gtatttgtgt gttactgtta aactcacaca tttgtgctaa
     5761 aaattagaga gttaaaattt ttctagttaa aagcttgaaa atttctataa aaatcggtat
     5821 tatattttcg aaagaaataa aaatattttc gaaagaaagg tggttacgat ggtaaataca
     5881 gaagtagcaa gaacaacaat caagacagaa tattttggca gccttactga aaggatgaac
     5941 aaatatcgag aagatgtttt aaataaaaaa ccttatattg atgctgagag agcagttcta
     6001 gcaacacgcg cctatgaacg atacaaggaa caacctaatg tcctaaaacg tgcatatatg
     6061 ctgaaagaaa ttttggaaaa tatgactatc tatattgaag aagaatctat gattgcggga
     6121 aatcaagctt cttccaataa agatgctcct atttttccgg aatatacgct agaatttgtt
     6181 ctcaatgagt tggatctttt tgaaaagcgt gatggagatg ttttctatat tacagaagaa
     6241 acaaaagaac aacttagaag tattgctccg ttttgggaaa ataataattt acgtgctaga
     6301 gctggtgcct tattacctga agaagtgtct gtttatatgg aaacaggatt cttcggtatg
     6361 gaaggtaaga tgaattctgg agatgctcac ctagcagtta actatcagaa acttttgcaa
     6421 tttggtttaa gaggttttga agagcgggct cgtaaagcaa aagtagctct agatttaaca
     6481 gatccagcaa gtattgataa atatcatttt tacgactcta tatttatcgt aatcgatgct
     6541 attaaagtat atgcaaagcg ctttgttgct cttgctaaaa gtttagccga aaatgcaaat
     6601 cctaaacgta agaaagaatt acttgagatt gcagatattt gctctagagt cccatatgaa
     6661 ccggcaacta cttttgcaga agctattcaa tcagtttggt ttattcaatg tattttacaa
     6721 attgaatcta atggccactc tctttcatat ggccgttttg atcaatatat gtatccatat
     6781 atgaaggctg atttagaaag tggtaaagaa acagaagata gcattgttga acgtctgaca
     6841 aatctttgga ttaagacaat tacaatcaat aaggttcgca gtcaatcaca tacattttct
     6901 tcagcaggaa gtcctttata tcaaaatgtt acaattggtg gacagactcg agataagaag
     6961 gatgctgtta acccattatc ttatttggta ttaaaatcag ttgcacaaac ccatctaccg
     7021 caacctaatc taactgtacg ttaccatgca ggtttagatg ctcgtttcat gaatgagtgt
     7081 attgaagtga tgaaacttgg ttttggtatg cctgcattta ataatgatga gattattatt
     7141 ccttctttta ttgcaaaagg agtattggaa gatgatgctt atgattacag tgccattgga
     7201 tgtgttgaaa cggcagttcc agggaaatgg ggctatcgtt gcacaggtat gagttatatg
     7261 aacttcccta aggttctact tatcacgatg aatgatggaa ttgatccggc ttcgggtaaa
     7321 cggtttgcac caagctttgg tcattttaag gatatgaaga acttttctga attagaaaat
     7381 gcttgggata aaacactaag atatttgaca cgaatgagtg ttattgttga aaattctatt
     7441 gatttatcat tggaacgaga agttcctgat attctatgtt cagcattgac tgatgattgt
     7501 attggtcgtg gaaaacacct taaagaaggt ggagcagtat atgattatat atcaggattg
     7561 caagttggaa ttgcaaattt gtcggattca ttagctgcaa ttaaaaaatt ggtgtttgag
     7621 gaagaacgta taagcccaag tcagctttgg catgcactgg aaacagatta tgccggagaa
     7681 gaaggtaagg tcattcaaga aatgttgatt catgatgcac ctaagtatgg taatgatgat
     7741 gattatgctg acaaattggt tactgctgct tatgacattt atgttgatga aattgctaaa
     7801 tatcctaata cacgttatgg aagagggcct attggaggaa ttcgttattc aggaacatct
     7861 tctatctcag ccaacgtagg gcagggacgt ggaacattag caactccaga tggacgcaac
     7921 gcgggtacac cgttagcaga gggttgttca ccatcacata atatggatca acacggccct
     7981 acatctgttt taaaatctgt ttcaaaatta ccaacagatg aaatcgtagg tggggttctc
     8041 ttaaatcaga aagtaaatcc tcaaacgtta gccaaagaag aagataaatt aaaactaatt
     8101 gctttgttac gaacattctt taatcgttta catgggtacc atattcaata caatgttgtt
     8161 tccagagaga cgttgattga cgctcagaaa catcctgaaa aacacagaga cttaattgtt
     8221 cgtgttgcag gatactctgc attcttcaat gttctttcta aggcaaccca agatgacatt
     8281 ataggacgta ctgagcatac tttgtaaaat aaagaggttc tttttatgga atttatgctt
     8341 gacacattaa atttagatga gattaaaaag tggtctgaaa ttttgccgct agctggggta
     8401 acttcaaatc ccactattgc aaaaagagag ggttctatta atttttttga acgaatcaaa
     8461 gatgtaagag aattgattgg ctctacaccc tctattcatg ttcaggtgat ttctcaagat
     8521 tttgaaggca tcttaaagga tgctcatgaa attcgaagac aagcaggaga tgatatattt
     8581 atcaaagtac ctgttactcc agctggatta cgtgcaataa aggtgctaaa aaaagagggc
     8641 taccatatca ctgcaacagc tatttataca gttattcagg gattattagc tatcgaagta
     8701 ggagcggatt acctagctcc atattataat agaatggaaa atctgaacat tgattcaaat
     8761 tctgtcattc gtcaattagc tcttgctatt gatagacaga actctcctag taagatttta
     8821 gctgcatcct ttaaaaatgt agcacaagta aataatgctt tagctgcagg tgcgcatgct
     8881 gttacagcag gagcagatgt ttttgaatca gctttcgcca tgccatctat ccaaaaggcg
     8941 gttgatgatt tttctgacga ttggtttgtt actcaaaata gtcgttccat ttagatagag
     9001 aggaaataca tatgagaatt tttgctagtc cttctagata tattcagggg gaaaatgcct
     9061 tgtttgaaaa tgccaaatca attttggatt tgggaaatta ccctattcta ttatgcgatc
     9121 agttggttta tgatattgtt ggaaaacgat ttgaagatta tctacatagg tatggtttcc
     9181 atattgttct ggcgctattt aatggtgaag cttctgacaa tgaaatcaat cgagttgttg
     9241 ccttggctga gaaagaaaat tgtgatagta ttatcggtct tggtggggga aagacgattg
     9301 atagcgcaaa agctattgca gatttgattg aaaagcctgt tattattgct ccaacaattg
     9361 catcgaccga cgcacctgta tctgctttat ctgttattta tacagatgaa ggtgcatttg
     9421 atcattatct attttattct aaaaatccag atttagtttt ggttgataca aaagttattt
     9481 cacaagcccc taagcgttta ttagcgtctg gtattgcaga tggtttagca acttgggttg
     9541 aggcgcgtgc ggttatgcag gcaaatggaa aaactatgtt gggacaacag caaacattgg
     9601 ctggagttgc aattgcgaag aaatgtgaag aaacgctgtt tgcagatggt ttacaggcta
     9661 tggcagcttg tgaagctaaa gtggtgacac cagcattaga aaatattgtt gaagctaata
     9721 ctttattgag tggcctaggt tttgaaagtg gaggattagc tgcggcgcat gcaattcata
     9781 atggttttac tgcattgaca ggtgacattc atcatttaac acatggtgaa aaagtagctt
     9841 atggaacttt agtacaacta ttattggaaa atagacctaa agaagaactt gataagtata
     9901 ttgagtttta caaaaaaatt ggtatgccaa caactctaaa agaaatgcat ttggatcaag
     9961 ttggatatga tgatttaata aaagttggta aacaagcaac tatggagggt gagacaattc
    10021 atcagatgcc gtttaagatt tcgccttcag atgttgctca agctattatc gctgtagatg
    10081 cctatgtaaa ttcaaaataa acaataagga ctactgtttt ccaaatggta gtcttttatt
    10141 gatcctcgta ttgaattcta tagcagattg aaataagatg agaacaaatc gattgggaaa
    10201 gtaaaattaa tttctataag tattttagca attgtttcgt actatttcag attcagtcca
    10261 ctatacgttc ttcataaatc aaaaagcgac ataggttgtc ggctatttat tgtgaataca
    10321 ttaattagca ttccagtttt atcttcggtc taaaataagt attttgtgct atacgagata
    10381 agtttcttga cttactcctt gatttactgc ataacaatgg gataaaaagt gggagataga
    10441 gcaattcata gtcatcaaaa ttaatgagat acagtataca gtttttcctt taaacacatt
    10501 tcaaattccc tcaaaaatgg tataatagta acatcacaaa attggagaga gaccatgagt
    10561 ttttacaatc ataaagaaat tgagcctaag tggcagggct actgggcaga acatcataca
    10621 tttaagacag gaacagatgc atcaaaacct aagttttatg cgcttgatat gttcccttat
    10681 ccgtctggag ctggtctgca cgtaggacac ccagaaggtt atactgcaac cgatatcctc
    10741 agtcgttaca aacgtgcgca aggctacaat gtccttcacc caatgggttg ggatgctttt
    10801 ggtttgcctg cagagcaata cgctatggat actggtaatg acccagcaga atttacagcg
    10861 gaaaacattg ccaacttcaa acgtcaaatc aatgcgcttg gattttctta tgactgggat
    10921 cgtgaagtca acacaacaga tccaaactac tacaagtgga ctcaatggat tttcaccaag
    10981 ctttacgaaa aaggcttggc ctatgaagct gaagtgccag taaactgggt tgaggaattg
    11041 ggaactgcca ttgccaatga agaagtgctt cctgacggaa cttctgaacg tggaggctat
    11101 ccagttgtcc gcaaaccaat gcgccaatgg atgctcaaaa tcacggctta cgcagagcgc
    11161 ttgctcaatg acttagatga actagattgg tcagagtcta tcaaggatat gcaacgcaac
    11221 tggattggta aatcaactgg tgccaatgta actttcaaag taaaaggaac agacaaggaa
    11281 tttacagtct ttactactcg tccggacaca cttttcggtg cgactttcac tgttttggct
    11341 cctgaacatg aattagtaga tgccatcaca agcacagagc aagcagaagc agtagcagac
    11401 tacaaacacc aagccagcct taagtcagac ttggctcgta ccgaccttgc caaagaaaaa
    11461 actggtgtat ggactggtgc ttatgccatc aatcctgtca atggtaagga aattccaatc
    11521 tggattgcgg attacgttct tgctagctat ggtacaggtg cggtaatggc tgtgcctgcc
    11581 cacgatcaac gtgactggga atttgccaaa caatttgacc ttccaatcgt cgaagtactt
    11641 gaaggtggaa atgttgaaga agctgcctac acagaagatg gccttcacgt taattcagac
    11701 ttcctagatg gactcaacaa agaagacgct attgccaaga ttgtggcttg gttggaagaa
    11761 aaaggctgtg gacaagagaa ggtaacctac cgtctccgcg actggctctt tagccgtcaa
    11821 cgttactggg gtgagccaat cccaatcatt cattgggaag atggaacttc aacagctgtc
    11881 cctgaaagtg aattgccact tgttttgcct gtaaccaagg atatccgtcc ctcaggtact
    11941 ggtgaaagtc cactagctaa cttgacagac tggcttgaag tgactcgtga agatggtgtc
    12001 aaaggtcgtc gtgaaacaaa tactatgcca caatgggcag gttcaagctg gtactacctc
    12061 cgctatattg acccacacaa tactgagaaa ttggctgacg aggatctgct caaacaatgg
    12121 ttgccagtag atatctacgt gggtggtgcg gaacatgctg tacttcactt gctttacgct
    12181 cgtttctggc ataaattcct ctatgacctc ggtgttgttc caactaagga accattccaa
    12241 aaactcttta accaaggaat gattttggga acaagctatc gtgaccaccg tggggctctt
    12301 gtggcgactg acaaggttga aaaacgtgat ggttctttct tccatgtgga aacaggggaa
    12361 gagttggagc aagcgccagc caagatgtct aaatcgctca agaacgttgt caacccagac
    12421 gatgtggtgg aacaatacgg tgctgatacc cttcgtgtct atgaaatgtt catgggacca
    12481 ctcgatgctt cgattgcttg gtcagaagaa ggtctggaag gaagtcgtaa gttccttgac
    12541 cgtgtttacc gtttgattag aagtaaggaa attgttgcgg aaaacaatgg cgctcttgac
    12601 aaggtttaca acgaaactgt taaagctgtc acagagcaaa ttgattccct caaattcaac
    12661 acagccattg cccaacttat ggtctttgtc aatgcggcta acaaggaaga caagctctat
    12721 gttgactatg ccaaaggatt tatccaattg attgccccat ttgcacctca cttggcagaa
    12781 gaactctggc aaactgttgc tgcaacaggt gagtcaatct cttacgtgac ttggccaaca
    12841 tgggacgaaa gtaaactggt tgaagacgaa atcgaaatcg ttgtccaaat caaaggtaaa
    12901 gtccgtgcca aactcatggt cgctaaagac ctatcacgtg aagaattgca agaaattgct
    12961 ctagcggatg aaaaagtcaa agcagaaatt gacggtaagg aaatcgtgaa agtgattagt
    13021 gtaccaaata aattggttaa tattgttgta aaataaccaa tttatttgct gatttccaac
    13081 ctcaaacagt ttcccaaact gtttgagcca actaaactcg ttaatatcgt tgtgaaataa
    13141 ggtaggaatc cttcagagta gaatctggag gattttttga atcttcttat gaaagtatgg
    13201 tatactatgg gcaactataa agtttgaaaa gtgaaaaaag gagaaagcta tgccagtaaa
    13261 tgaatatggt caaatgattg gggagtcaat ggaaggttat acaccaggtg aactgccttc
    13321 tattgatttc ttagaagggc gttatgctag gatagaggct ctttcagtgg aaaagcatgc
    13381 ggaggatttg ctagctgttt atgggcctga tacgcctcag gagatgtgga cctacctttt
    13441 tcaggagcca gtagcagata tgggggagct ggttagcctt ttacatcaga tgttggctcg
    13501 taaggaccgt ttttactatg caatcataga caaggcgact ggtaaggctt tgggatcttt
    13561 ttctctcatg cgcattgacc agaataaccg agtaatagaa gtcggagcag tcactttttc
    13621 tccagagcta aggggtacac ggataggaac tgaagctcag tatctcctag ctcgctatgt
    13681 ttttgaggag ctaaactatc gtcgctacga gtggaaatgc gatgccttaa atctgccatc
    13741 cagacgagct gcggagcgtt tgagctttat ttatgaagga accttccgtc aggcagtggt
    13801 ttataaggga cgtacgagag atacggattg gttgtctatg attgataagg actggcctca
    13861 agtcaaagat cgtttggaaa catggttgcg tcctgaaaat tttgataaaa atggacagca
    13921 gtacaagagc ttgagagaac tttaagaggt gttgagatga ttactattaa aaagcaagaa
    13981 attgtcaagc tagaggatgt tttacatctc tatcaggctg tcggttggac aaattatacc
    14041 catcaaccag agatgctgga gcaggcctta tctcattcat tagtaattta tctggcactt
    14101 gatggtgatg ctgtggtggg cttgattcgt ttggttggag atggattctc atcagtattg
    14161 gttcaggatt taatcgtttt accaatctat cagcgtcaag gaattggtag tgccttgatg
    14221 aaagaggctt tagaggatta caaagatgcc tatcaagtcc agctggtgac agaagagaca
    14281 gaaagaacct tgggcttcta tcgttctatg ggctttgaaa tcttatccac ctataattgt
    14341 atagggatga cttggatgaa tcgtaaaaaa taacaaaact tgttttttct taagcaaaat
    14401 ttaaggatgg tctagtatta tatagtcatt aaataaagac ctcctaactt tatttaataa
    14461 aatcctaaac ttttcttttt cataataatc tcccttaact ccacccaatc aggtggagtt
    14521 ttttagctct atttcaggct tttggggact attctaaaaa taatttttcg atatttttcg
    14581 gtatttttcg gattttggtc ggggaattgg cggggacttt tttagcgaat ataactaaga
    14641 aataggtctg tggtcgcttc agctagttca aactcaattt gattgtaagt gactgttaat
    14701 tgaagaactg gaactactgt aataatgatt ttgcttgtct agtagtggcg tattaaatga
    14761 gaagtgacat gtaagattgt ctcttcgttt acgaaatcaa aatttctatg aaattgatag
    14821 aatccctaac gttctcttaa tcttaaccgt cttgtcgtac agcggttgaa gaaaatgtaa
    14881 acctttgaca aaattcgtga actatgggat aataaaaagg aattgagtac tagttctata
    14941 tcataggcta gaaaagaccc caagctcacg accaaataag cttgaggtct tttttgacac
    15001 tatttgtcct tgtttagcca tttatcgact atacgaagaa cgacaccgac cacaattggt
    15061 ccgatgatag ttttgagtac taattccatc atgggctatc tcacctcctt tcgtaggcgg
    15121 tgtagcagtg tcgtagaata ttataccaca taagtgctaa acttaggagt cacccaatca
    15181 ggtggctttt ttgtttgtgg cttggttttt tgatataata gagccatgag tagaatttta
    15241 gataatgaga taatggggga tgaggagtta gtagaacgca cgctccgtcc tcagtattta
    15301 cgtgaatata tcggacagga taaggtcaag gaccagctac aaatctttat tgaagctgcc
    15361 aaaatgcggg atgaagcgct ggatcatgtg ctcttatttg ggcctccagg tctcgggaaa
    15421 acgaccatgg cctttgttat tgccaacgaa ctgggagtca atcttaagca gacgtcgggt
    15481 ccagtcattg aaaaagccgg agatctggta gctattttga atgagttaga gcctggggat
    15541 gtacttttta ttgatgagat ccatcgtttg ccaatgtcag tggaagaggt gctttatagt
    15601 gctatggagg acttctacat agatattatg attggggctg gtgagggtag tcgtagtgtt
    15661 catttggagt taccaccttt taccttgatt ggtgcgacga ctcgggctgg tatgctctcc
    15721 aatccgctac gggcacgttt tgggattaca ggccatatgg agtattatgc ccatgctgac
    15781 ttgacagaaa ttgtcgagcg gacggcagat atttttgaga tggaaatcac tcatgaggca
    15841 gcatctgagt tggccctacg tagtcgtggg acccctcgta ttgccaatcg tctcctcaag
    15901 cgcgtgcgcg attttgccca gataatgggg aatggggtaa ttgatgatat tattaccgat
    15961 aaggctttga ctatgctgga tgttgaccat gaaggtttgg actatgtgga tcaaaaaatc
    16021 cttcgtacca tgattgagat gtacagtgga ggacctgttg gtctaggaac tctttctgtg
    16081 aatatcgccg aagagcgtga gacagttgaa gatatgtatg agccttactt gattcaaaaa
    16141 ggttttatca tgcggacacg gtctggacgg gtggcgactg ctaaggcata tgagcactta
    16201 ggttatgaat acagtgaaaa ataagcaaga aatcttagag gcttttagag aaaatccaga
    16261 tatgatggcc attctgacga tcatccgaga ccttggtctg aaagactcgt ggttggcagc
    16321 aggttctgtc agaaatttca tttggaatct tttgtcagac aaatcccctt ttgatcatga
    16381 aacagatata gatgtgattt tctttgatcc agatttttct tatgaggaaa ccttattact
    16441 ggagaaaaag ctgagagagg attttcctca gtaccagtgg gaattgaaaa atcaggtcta
    16501 tatgcatcag cacagtcctc acactgcttc ctataccagt tctcgtgatg ctatgagtaa
    16561 gtatccagaa cggtgtacgg cagttggact gcgcttgaat gaagaatcag attttgaact
    16621 ctatgcacct tatggtttgg aggatatttt gaattttcaa gttcgtccaa ctcctcattt
    16681 cttagaaaat gaagaccgaa tggaactcta tcaaacacgt ttatccaaga aaaattggca
    16741 ggagaaatgg aaaaatttga tttttaaaaa tacttaagga aactttaagc tagggagtgt
    16801 acactaagtt cataagttaa gaagacctta acttaaactc ctaaaacttt ttcataataa
    16861 tctccctata aaaataaagt cgcccaatca ggcggcttat ttttttgaaa aatgggcatg
    16921 gtgcctgaga ataaatagct tagtgataga agaaaatggg gaaatatggt ataatgaaac
    16981 gatagatttt tgaataggaa taagatcatg tttggatttt ttaagaaaga taaggctgtg
    17041 gaagtagagg ttccgacaca ggttcctgct catatcggca tcatcatgga tggcaatggc
    17101 cgttgggcta aaaaacgtat gcaaccgcga gtttttggac ataaggcggg catggaagca
    17161 ttgcaaaccg tgaccaaggc agccaacaaa ctgggcgtca aggttattac ggtctatgct
    17221 ttttctacgg aaaactggac ccgtccagat caggaagtca agtttatcat gaacttgcca
    17281 gtagagtttt atgataatta tgtcccggaa ctacatgcga ataatgttaa gattcaaatg
    17341 attggggaga cagaccgcct gcctaagcaa acctttgaag ctttaaccaa ggctgaggaa
    17401 ttgactaaga acaacacagg attgattctt aattttgctc ttaactatgg tggacgtgct
    17461 gagattacac aggcgcttaa gttgatttcc caggatgttt tagatgccaa aatcaaccca
    17521 ggtgacatca cagaggaatt gattggtaac tatctcttta ctcagcattt gcctaaggac
    17581 ttacgagacc cagacttgat tatccgtact agtggagaat tacgtttgag caattttctt
    17641 ccatggcagg gagcctatag tgagctttat tttacggaca ccttatggcc tgattttgac
    17701 gaagcggcct tgcaggaagc tattcttgcc tataatcgtc gtcatcgccg atttggagga
    17761 gtttaggagg aaatatgacc caggatttac agaaaagaac cttgtttgca gggattgccc
    17821 tggctatttt cctaccaatt ttaatgattg ggggcctctt gcttcagata gcaatcggaa
    17881 tcatagccat gctagccatg catgaacttt tgaagatgag aggtctagag accatgacga
    17941 tggagggcct cttgaccctc tttgcaacct ttgcattgac cattcccttg gagaattacc
    18001 tgactttttt gccagttgat gggaatgtgg ttgcctatag tgttttgatt tcaatcatgt
    18061 taggaacgac cgtttttagc aagtcttata cgattgagga tgcggttttc cctcttgcta
    18121 tgagcttcta cgtgggcttt ggatttaatg ctttactaga tgctcgtgtt gcaggtttgg
    18181 acaaggctct cttagccttg tgtatcgtct gggcgacaga cagtggtgcc tatcttgttg
    18241 ggatgaacta tgggaaacga aagttagcac caagggtatc gcctaataaa acccttgagg
    18301 gtgccttggg tggtatttta ggagcaattt tagtaaccat tatctttatg atagttgaca
    18361 gtacagttgc tcttccatat ggaatttaca agatgtcagt ctttgctatt ttctttagca
    18421 ttgctggaca atttggtgat ttactagaaa gttcgatcaa acgtcatttt ggtgttaagg
    18481 attctgggaa atttatccct ggacatggtg gtgttttgga tcgtttcgat agtatgttgc
    18541 ttgtatttcc aatcatgcac ttatttggac tcttttaatc aaaagacgga ggaaacgcta
    18601 tgctcggaat tttaaccttt attctggttt tcgggattat tgtagtggtg cacgagttcg
    18661 ggcacttcta ctttgccaag aaatcaggga ttttagtacg tgaatttgcc atcggtatgg
    18721 gacctaaaat ttttgctcac attggcaagg atggaacggc ctataccatt cgaatcttgc
    18781 ctctgggtgg ctatgtccgc atggccggtt ggggtgatga tacaactgaa atcaagacag
    18841 gaacgcctgt tagtttgaca cttgctgatg atggtaaggt taaacgcatc aatctctcag
    18901 gtaaaaaatt ggatcaaaca gccctcccta tgcaggtgac ccagtttgat tttgaagaca
    18961 agctctttat caaaggattg gttctggaag aagaaaaaac atttgcagtg gatcacgatg
    19021 caacggttgt ggaagcagat ggtactgagg ttcggattgc acctttagat gttcaatatc
    19081 aaaatgcgac tatctggggc aaactgatta ccaattttgc aggtcctatg aacaatttta
    19141 tcttaggtgt cgttgttttt tgggttttaa tctttatgca gggtggtgtc agagatgttg
    19201 ataccaatca gttccatatc atgccccaag gtgccttggc caaggtagga gtaccagaaa
    19261 cggcacaaat taccaagatt ggctcacatg aggttagcaa ctgggaaagc ttgatccaag
    19321 ctgtggaaac agaaaccaaa gataagacgg caccgacttt ggatgtgact atttctgaaa
    19381 aggggagtga caaacaagtc actgttacac ccgaagatag tcaaggtcgt taccttctag
    19441 gtgttcaacc gggggttaag tcagattttc tatccatgtt tgtaggtggt tttacaactg
    19501 ctgctgactc agctctccga attctctcag ctctgaaaaa tctgattttc caaccggatt
    19561 tgaacaagtt gggtggacct gttgctatct ttaaggcaag tagtgatgct gctaaaaatg
    19621 gaattgaaaa tatcttgtac ttcttggcaa tgatttccat caatattggg atttttaatc
    19681 ttattccgat tccagccttg gatggtggta agattgtgct caatatccta gaagccatcc
    19741 gccgcaaacc attgaaacaa gaaattgaaa cctatgtcac cttggccgga gtggtcatca
    19801 tggttgtctt gatgattgct gtgacttgga atgacattat gcgactcttt tttagataat
    19861 cgaggaatat tatgaaacaa agtaaaatgc ctatcccaac gcttcgcgaa atgccaagcg
    19921 atgctcaagt tatcagccat gctcttatgt tgcgtgctgg ttatgttcgc caagtttcag
    19981 caggtgttta ttcttatcta ccacttgcca atcgtgtgat tgaaaaagct aaaaacatca
    20041 tgcgccaaga attcgaaaag attggtgctg ttgagatgtt ggctccagcc cttcttagtg
    20101 cagaattgtg gcgtgaatca ggtcgttacg aaacctatgg tgaagacctt tacaaactaa
    20161 aaaaccgtga aaaatcagac tttatcttag gtccaactca cgaagaaacc tttacagcta
    20221 ttgtccgtga ttctgttaaa tcttacaagc aattgccact caacctttat caaattcagc
    20281 ccaagtatcg tgatgaaaaa cgcccacgta atggacttct tcgtacacgt gagtttatca
    20341 tgaaggatgc ttatagtttc cacgctaact atgatagttt ggatagtgtt tatgatgagt
    20401 acaaagcagc ctatgagcgt attttcactc gtagtggttt agacttcaag gctattattg
    20461 gtgacggtgg agccatgggt ggtaaggata gccaagaatt tatggccatt acatctgctc
    20521 gtacagacct tgaccgctgg gttgtcttgg acaagtcagt tgcctcattt gacgaaattc
    20581 ctgcagaagt gcaagaagaa atcaaggcag aattgctcaa atggatagtc tctggtgaag
    20641 ataccattgc ttactcaagt gagtctagct atgcagctaa cttagaaatg gcaacaaacg
    20701 agtacaaacc aagcaaccgt gttgtcgctg aagaagaagt tactcgtgtt gcaacgccag
    20761 atgttaaatc aattgatgaa gttgcagctt tcctcaatgt tccagaagaa caaacgatta
    20821 aaaccctctt ctacattgca gatggtgagc ttgttgcagc ccttctagtt ggaaatgacc
    20881 aactcaacga agtcaagttg aaaaaccact taggagcaga tttctttgac gttgctagcg
    20941 aagaagaagt ggcgaatgtt gttcaagcag gatttggttc acttggacca gttggtttgc
    21001 cagagaatat taaaattatt gcagatcgta aggtgcaaga tgttcgcaat gcagttgtcg
    21061 gtgctaacga agatggctac cacttgactg gtgtgaaccc aggccgtgat tttactgcag
    21121 aatatgtgga tatccgtgaa gttcgtgagg gtgaaatttc cccagatgga caaggtgtcc
    21181 ttaactttgc gcgtggtatt gagatcggtc atattttcaa actcggaact cgctactcag
    21241 caagcatggg agcagatgtc ttggatgaaa atggtcgtgc tgtgccaatc atcatgggat
    21301 gttacggtat cggtgtcagc cgtcttcttt cagcagtgat ggagcaacac gctcgcctct
    21361 ttgttaacaa aacgccaaaa ggtgaatacc gttacgcttg gggaatcaat ttccctaaag
    21421 aattggcacc atttgatgtg catttgatta ctgtcaatgt caaggatgaa gaagcgcaag
    21481 ccttgacaga aaaacttgaa gcaagcttga tgggagctgg ttatgaagtc ttgacagatg
    21541 accgtaacga acgtgtcggt gttaaattta gcgatagtga cttgattggt cttccaatcc
    21601 gtatcactgt tggtaaaaaa gcggccgatg gcattgtaga agttaagatc aaggcaactg
    21661 gtgacaccat tgaggttcat gcagataacg tgcttgaaac gcttgaaatc ctaagcaaga
    21721 aataaaaact ataatcagaa gaaaaacaag gaaaaaatgt aactagtttt taccttgttt
    21781 tttctgtata atgggagaaa tgagtagata aagaggtaaa tgttatgcta agatttccaa
    21841 aggattttgt ctggggatcc tctacttctg gaccgcagac agaaggacgt gtagctggtg
    21901 acggtaaggg agacaatctc tgggattact ggttccaagt ggagccaaat cgttactata
    21961 atgggattgg ttcagataag acatcgactt tttatgaaaa ttgggagcgg gatattgagc
    22021 ttttgttaga gactggtcac acagtctttc ggacttctat tcagtggtca cggatttttc
    22081 cacaaggctg tggaaaagtc aaccctcaag gtgtggattt ttatcgtaag gtctttgagg
    22141 ctattaaggc taaaggaatt cgtctgttag tcaatctcta tcattttgat ttaccttttg
    22201 cccttcaaga ggatggtgat ggttgggaaa ataaggcgac agtctcagcc tatgaagact
    22261 atgctcgttt ttgttttgag acttatggag atttagtgga tcaatggatt acctttaacg
    22321 agctcatcgt tcctgtagaa tttggttatt tttatgatgc ccattatcca cataaggtgg
    22381 atgcagaggc agctgttaag gtagcctatc atacacaatt ggccagcagc cgggctgtta
    22441 aggcttgcca tgaacttttg cctgattcca agattgggat tgtcctcaac ttgacaccgg
    22501 cttatccacg tagccagcat cctgctgatg tcaaggcagc tcgtattgcg gccctttttc
    22561 aggcccaatc tttcttagat ccatctgtct tggggactta tccacaggag ttggtagaaa
    22621 tcttgcatga acacggtctt ttacctgatg ctacagagga agagttggaa ctcattcatg
    22681 ataatacggt ggacttcctt ggtgtgaact actatcaacc tttgcgtgtt atggctcctc
    22741 gatttgctaa gcatccagag agtccactct taccagaaca tttttacgag ccttatgtga
    22801 tgcctggacg taaaatcaat tctcaccgtg gttgggagat ttatgagcaa gggatttatg
    22861 acatcgccca aaatatcaag gaaaattatg gcaatattga atggatgttg actgagaatg
    22921 gtatgggtgt tgaaggggaa gaaaaattcc gtcaagatgg aatgattcaa gatgattacc
    22981 gtattgactt tgtaaaaggt catcttcgtg aacttcaccg tgccattgaa gatggtgcca
    23041 actgtaaggg ctacttgatt tggaccttta ttgactgctg gtcatggctc aacagctata
    23101 aaaatcgcta tggtttggtc gaattagact tggaaacgca agaacgtcgt ctgaagaaat
    23161 caggccactg gttcaaaaaa ttaagcgata ataatggatt ttaaaaagga ctctgactga
    23221 ttatcccaat cggtcagagt tttttgctta caggagctta atttgaacta taccaatttt
    23281 tactcttgac aagtgaaaga aacaagtata tactgttttt gctgattcta taaaagatga
    23341 attagactat accaatttta aggagaaagc acagcttgcc tgtgtcgtat atactatgtg
    23401 tggaattgtt ggtgttgttg gaaacacaaa tgcaactgat attttgattc aagggcttga
    23461 aaagcttgaa taccgtggct atgattctgc gggaattttt gtcctagatg gtgctgataa
    23521 ccatttggtg aaggctgttg gtcgtatcgc agaattgtct gccaagacag ctggtgttga
    23581 gggaacaact ggtatcggac atactcgttg ggcaactcat gggaaaccaa cggaagacaa
    23641 tgctcaccca caccgctctg agacagaacg ttttgtcttg gttcacaatg gggtgattga
    23701 aaactacctt gaaattaaag aagaatacct tgctgggcac cacttcaaag ggcaaacaga
    23761 tacggaaatc gccgtacatt tgattggaaa atttgcggaa gaagacggtc tctcagttct
    23821 tgaagccttt aaaaaagctc ttcatattat ccgtggttca tatgcctttg ccttgattga
    23881 ctctgaaaat ccagatgtca tctatgtagc gaaaaacaaa tctccacttt tgattggtct
    23941 tggggaaggc tacaatatgg tctgctcaga tgctatggct atgattcgtg aaaccaacca
    24001 atacatggaa attcatgacc aagagttggt aatcgtcaag gctgatagcg tggaagttca
    24061 agactatgat ggaaatcgtc gtgagcgtgc tagctacact gctgaacttg acttgtcaga
    24121 tatcggtaag ggaacttacc cttactacat gctcaaggaa attgacgagc aaccaactgt
    24181 tatgcggaaa ctcatccaag cctacacaga tgaggctggt caagttgtcg tagacccaga
    24241 tatcattaag gctgttcaag acgcagaccg catctacatc cttgcagctg gaacatctta
    24301 ccatgcagga tttgcttcta agaagatgtt ggaagaattg acagatacac cagttgaact
    24361 tggaatctca tctgagtggg gctacggtat gccacttctc agcaagaaac cactcttcat
    24421 ctttatcagc caatctggtg aaacagcgga tagtcgtcaa gttttggtca aggctaatga
    24481 aatgggaatt ccaagcttaa cagtgacaaa tgttccaggt tcaaccctct cacgtgaagc
    24541 caactatacc atgctccttc acgcaggtcc tgaaattgcc gtggcatcaa ctaaagccta
    24601 tacagcgcaa atcgcagccc ttgccttcct tgcaaaagca gtcggagaag caaatggtaa
    24661 tgctaaagcg caagcctttg acctggttca tgaattgtca atcgtagctc agtctattga
    24721 atcaactctt tcagagaaag aaaccattga agccaaggtt cgtgaacttc ttgaaacaac
    24781 tcgtaacgcc ttttacatcg gacgtggtca agattattac gtagccatgg aagcaagtct
    24841 caaactcaaa gagatttctt atatccagtg tgaaggtttt gcggcaggag aactcaagca
    24901 cggaaccatt gccttgattg aagaaggaac gcctgttttg gctctcttgt cagatccagt
    24961 tcttgccaac catactcgtg gaaatatcca agaggtcgca gcccgtggtg ccaaagtcct
    25021 cactatcgca gaagagaatg tagccaaaga taccgacgat atcgtcctta cgaccgtaca
    25081 tccatacctc tcaccaattt caatggtcgt accaacgcaa ttagtcgctt actttgcaac
    25141 cctccaccgt ggcctcgatg tggacaaacc acgtaacctt gccaagtcag taacggtaga
    25201 ataagctaaa aaagtctagt tatctaggct ttttcttgtg agcaaattgg ttgcttgtac
    25261 tcaagattcg tgttatactc ttagaaaatc tcttcaaacc acgtcaccgt cgccttgccg
    25321 taggtatggt tactga
//
LOCUS       DAVBCB010000029        24327 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVBCB010000029 DAVBCB010000000
VERSION     DAVBCB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24327
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.029"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..618)
                     /locus_tag="VQU28_001147"
     CDS             complement(<1..618)
                     /locus_tag="VQU28_001147"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HET2476508.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEK"
     gene            complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQU28_001148"
     CDS             complement(769..2079)
                     /gene="secY"
                     /locus_tag="VQU28_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HET2476509.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQU28_001149"
     CDS             complement(2092..2532)
                     /gene="rplO"
                     /locus_tag="VQU28_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HET2476510.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQU28_001150"
     CDS             complement(2677..2901)
                     /gene="rpmD"
                     /locus_tag="VQU28_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HET2476511.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQU28_001151"
     CDS             complement(2873..3367)
                     /gene="rpsE"
                     /locus_tag="VQU28_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HET2476512.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQU28_001152"
     CDS             complement(3385..3741)
                     /gene="rplR"
                     /locus_tag="VQU28_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HET2476513.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQU28_001153"
     CDS             complement(3825..4361)
                     /gene="rplF"
                     /locus_tag="VQU28_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HET2476514.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQU28_001154"
     CDS             complement(4553..4951)
                     /gene="rpsH"
                     /locus_tag="VQU28_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HET2476515.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQU28_001155"
     CDS             complement(5236..5505)
                     /gene="rpsN"
                     /locus_tag="VQU28_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HET2476516.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQU28_001156"
     CDS             complement(5523..6065)
                     /gene="rplE"
                     /locus_tag="VQU28_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HET2476517.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQU28_001157"
     CDS             complement(6089..6394)
                     /gene="rplX"
                     /locus_tag="VQU28_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HET2476518.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQU28_001158"
     CDS             complement(6472..6840)
                     /gene="rplN"
                     /locus_tag="VQU28_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HET2476519.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQU28_001159"
     CDS             complement(6866..7126)
                     /gene="rpsQ"
                     /locus_tag="VQU28_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HET2476520.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            complement(7151..7357)
                     /locus_tag="VQU28_001160"
     CDS             complement(7151..7357)
                     /locus_tag="VQU28_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HET2476521.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQU28_001161"
     CDS             complement(7367..7780)
                     /gene="rplP"
                     /locus_tag="VQU28_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HET2476522.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQU28_001162"
     CDS             complement(7784..8437)
                     /gene="rpsC"
                     /locus_tag="VQU28_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HET2476523.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQU28_001163"
     CDS             complement(8450..8794)
                     /gene="rplV"
                     /locus_tag="VQU28_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HET2476524.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQU28_001164"
     CDS             complement(8806..9087)
                     /gene="rpsS"
                     /locus_tag="VQU28_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HET2476525.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQU28_001165"
     CDS             complement(9191..10024)
                     /gene="rplB"
                     /locus_tag="VQU28_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HET2476526.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            complement(10042..10338)
                     /locus_tag="VQU28_001166"
     CDS             complement(10042..10338)
                     /locus_tag="VQU28_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HET2476527.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQU28_001167"
     CDS             complement(10338..10961)
                     /gene="rplD"
                     /locus_tag="VQU28_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HET2476528.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQU28_001168"
     CDS             complement(10986..11612)
                     /gene="rplC"
                     /locus_tag="VQU28_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HET2476529.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQU28_001169"
     CDS             complement(11828..12136)
                     /gene="rpsJ"
                     /locus_tag="VQU28_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HET2476530.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            complement(12402..13019)
                     /locus_tag="VQU28_001170"
     CDS             complement(12402..13019)
                     /locus_tag="VQU28_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HET2476531.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQU28_001171"
     CDS             complement(13016..13606)
                     /gene="nrdG"
                     /locus_tag="VQU28_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HET2476532.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKEIYEQVKRE"
     gene            complement(13599..14105)
                     /locus_tag="VQU28_001172"
     CDS             complement(13599..14105)
                     /locus_tag="VQU28_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476533.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDVEDFSYEEWL
                     EGNRNKEMGIWLPENRVPSIQFVLFGESGCALGFLNLRLRLNEGLLNYAGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            complement(14149..14289)
                     /locus_tag="VQU28_001173"
     CDS             complement(14149..14289)
                     /locus_tag="VQU28_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476534.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQU28_001174"
     CDS             complement(14301..16508)
                     /gene="nrdD"
                     /locus_tag="VQU28_001174"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HET2476535.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNCRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            complement(16628..18166)
                     /locus_tag="VQU28_001175"
     CDS             complement(16628..18166)
                     /locus_tag="VQU28_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2476536.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            complement(18203..18346)
                     /locus_tag="VQU28_001176"
     CDS             complement(18203..18346)
                     /locus_tag="VQU28_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476537.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQU28_001177"
     CDS             complement(18424..19956)
                     /gene="cls"
                     /locus_tag="VQU28_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HET2476538.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            complement(20102..20659)
                     /locus_tag="VQU28_001178"
     CDS             complement(20102..20659)
                     /locus_tag="VQU28_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HET2476539.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRAQQVQQPVAQQQVQQPAQQPAQQNTNTANAGGNQNQAAPVQNQPVAQP
                     TDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKI
                     YTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            complement(20656..21906)
                     /locus_tag="VQU28_001179"
     CDS             complement(20656..21906)
                     /locus_tag="VQU28_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HET2476540.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRIQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFEMLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNFSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSCREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            complement(22114..22419)
                     /locus_tag="VQU28_001180"
     CDS             complement(22114..22419)
                     /locus_tag="VQU28_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HET2476541.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQU28_001181"
     CDS             complement(22435..22854)
                     /gene="ruvX"
                     /locus_tag="VQU28_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HET2476542.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPLDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            complement(22858..23124)
                     /locus_tag="VQU28_001182"
     CDS             complement(22858..23124)
                     /locus_tag="VQU28_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HET2476543.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            complement(23211..23780)
                     /locus_tag="VQU28_001183"
     CDS             complement(23211..23780)
                     /locus_tag="VQU28_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2476544.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQU28_001184"
     CDS             complement(23846..24244)
                     /gene="spx"
                     /locus_tag="VQU28_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HET2476545.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
BASE COUNT         6740 a         5398 c         4560 g         7629 t
ORIGIN      
        1 tttttcaata tctgagaaga catcattgat atcttgatta ccttcgatgt catgaaccaa
       61 acctttggca cggtagtgag caatgattgg ttctccttga gcaatattaa catccaaacg
      121 acgttttact gtctcaggct tatcatcttc acgttggtag taatcttctt ctttatagtc
      181 aactggtggg ttaaagacct tgtggaaagt ttctccagtt acgcggtgga tgatacggcc
      241 actcaaacgt tccaagaggc tgtcagggtt cacttcaata ttgataatac cttctagttc
      301 aatgccaagt tcagccaatg ttttgtccaa ggcatgagct tgttcaattg tacgtgggta
      361 accatccaat aagaatcctg tttctttaat atcatcttgt gaaaggcgtt cttttacgat
      421 tccatttgta acttcgtcag gaaccaattc acccttgtca atatatgact tagcaagaac
      481 acccatttca gtttgatttg ccattgcagc gcggaacata tcacctgttg agatatgtgc
      541 aacatggaat tgttctacga tttttgctgc ttgagttccc ttacctgcac caggtaagcc
      601 cataatcaaa agattcatga tttgatctcc ttattttatt tttaaataga agcaaaaagt
      661 cttttcaccc ctatttaaaa acaaaataga agaggggaga tccaactctt acaaatgtca
      721 gagtttaaac ctccactccc tcagtattta ctgattcagt aaatactttt attctgttct
      781 gtccatgaaa ccaacatact tacgtttcaa taggtaacct tccaattgct tgattccttc
      841 gatacctgta gagataatga tcaagagact tgttccacca aaggcaacaa catcagaaag
      901 accaaataca tctttagctg caatcggtaa aatggaaatc acaccaagga agagggaacc
      961 aacagttgca agacgacgaa gaagtttaga catatattct tctgtacctt taccaggacg
     1021 aactccatgg atataggcac cactcttttg taggctctct gctgcttttt caggattaat
     1081 ctgtacaaac gtatagaaga atgtaaagag aataatcaac aaagcataca tggcaatacc
     1141 agttggagaa gtagttgcca acatctcttg tgctaccctt acccaagccc aatcatgacc
     1201 tgtggcactc aaaaactgaa gaatagccgc aggcgctgca gtaatcgaac tggcaaagat
     1261 aacagggata actccagcag ggtttacctt taacggaagg taagagctag atggagcacc
     1321 ttgtgcaacc ttagtatatt ggattggaat tttgtattct gcttgttgaa cataagttgt
     1381 aaagtaaata atcaacaata cagtaataat caaaatgatt acgaaaatga tagatgaagt
     1441 gatacggcta cttgggacgt tcacaaagta gtccacatag atgccctgaa tcatctctgg
     1501 aattgaggaa acaatcccgg caaagataat catggaaaca ccgtttccgt atcccttatc
     1561 tgtaatttgc tcacccaacc aagtgacaat catactacca gctgttaaga tgataccaat
     1621 cgtcagaaaa acttgtggag ttaaagcagt tttaatcaat tgagctccag ccaaggtatt
     1681 aaaaccagct gtaatcccga tagattgcac aaaagcgaga actagagcaa tataacgagt
     1741 agcttgattc aattttcttc gacctacttc cccttgttta ccccactcta caaacttggg
     1801 taaaatatcc atttgcaaga gttggacaac aatagaagcg gtgatatagg gactaactcc
     1861 tagggcaaaa atcgaaaagt tttttagggc attccccgac accaagctca acatgtttaa
     1921 gaaggataat ccacttaaag cattcaagct attggcattc acaccaggaa ctgtaatgct
     1981 agttccgata cgaaagacca aaacgataaa aattgtaaat aaaatttttg atcgaacctg
     2041 cttgacttta agagcttctc ttaataattt aaaaaacata ggtcacctct cttagatgac
     2101 ttctactgaa ccacctttag cagtgatagc ttcttcagct gatttagaga atttagctgc
     2161 tttcacagtc aatttcttag tcaactcacc gttaccaaga attttaatac ctgacttttc
     2221 agctttaaca attcctgctt cgataagaac aactggagtt acttcagcac catcttcaaa
     2281 gacgttcaat tggtcaaggt tcacaattgc gtattcttta gcgttgatgt tagtgaatcc
     2341 acgttttgga agacgacgga acaatggagt ttgtccacct tcaaaaccaa ggcgaactcc
     2401 gccaccgcta cgagcttttt gacctttttg accacgacca gatgttttac cgttacctga
     2461 tgaagtacca cgaccaacgc ggttacgtac tttacgagaa ccttctgcag gtttcaattc
     2521 atgaagtttc atttttattt tctcctcttt tgtaaaatgc tagcgccgat aagggagaaa
     2581 aggttgtctc ccccatcaac tcgcctatac gatgtcatca tagatgacta tatctagttt
     2641 taggggatgg tacagtgcac atcccctaaa acttcattag tttacttctt caactgttac
     2701 taagtgagat actgctgtaa tcataccacg gatagcagcg ttatcttctt taataacaga
     2761 gctgttcaat ttgccaagtc caagtgctac aacagtttta cgttgtgatg gaatgcgtcc
     2821 gattggagac ttagtcaaag taattttaat ttgagccatt ttatcccctt tcttatgcca
     2881 aatcagaaac tgaaatacca cgaagggcag caatttcttc agcgcgtttc aattgtttca
     2941 aaccttcaac agttgcacga acaatgttga ttggagtgtt agaaccaagt gatttagatg
     3001 taatatctgc cacacctgcc aattccacaa cggcacgaac tgcaccacca gcggcaactc
     3061 cagaaccttc tacagcaggt ttcaacaata ctttagctcc accgaattct gaaagaactt
     3121 cgtgtgggat tgttgttcca accataggaa cttcgatcaa gtttttctta gcatcatcta
     3181 ctgctttacg gattgcttct ggaacttctt gagctttacc agtaccaaat cctacgcgac
     3241 cattgtggtc accaacaaca acaagagctg cgaaacgaag acgacgtcca cctttaacaa
     3301 cttttgtaac acggttgaca gcaactacgc gttcttctaa ttcaactgca ttgtctttaa
     3361 atgccatttt ctagtgtcct cctattagaa tttcaatccg ttttcacgag ctgcatcagc
     3421 caaagctttc acacgtccgt gatatagata tccaccgcgg tcgaacacca cttctgaaat
     3481 acctttagcg tttgcacgtt ctgcaacgag tttaccgaca gcaacggctt gttcagtttt
     3541 agttcctttt gaaacttctt tatcaagagt tgaagcactt gcgagcgtta cacccgctac
     3601 gtcatcaatc acttgagcgt agatgcctgt attagaacgg aatacgttca aacgtgggcg
     3661 atcagcagtt ccagagagtt ttccgcgaac gcgacggtgg cgtttttggc ggagtttgtt
     3721 tttatctggt tttgaaatca cagttttcac ctctttagtt ttaaatcgtg tgctatgcac
     3781 aaagttggaa aataggttgg tggttgatga tcaaccactc aacattattt acctgtttta
     3841 ccttctttac ggcgaacgaa ttcaccaacg taacggatac ctttaccttt atatggttct
     3901 ggtgaacgaa ggctacgtac gtaagcagct gtttgaccaa ctacttcttt tgaaattccg
     3961 ctaacaacga ttgttgttgg gtttggaagt tcaaaagtaa ttccttctgg agcttcaact
     4021 tcgtctggat gagatttacc aacagccaaa acaagtttag atccttgaag ctgtgcacgg
     4081 taaccaaccc cacgcatttc aagttctttc ttgaatcctt ctgatacacc aacaaccatg
     4141 ttgttcaaaa gggcacgagt agttccgtgg atagttttca tttcttttga atcgtttgga
     4201 cggtgaagag ttatttcagt accttccaca cggatttcaa tatcttttga gaactcacga
     4261 gtaagttctc ctttaggtcc ttttacagtt acaacgttgt cattgttagc gagttcaaca
     4321 ccagcaggca acacgataac tttattacca atacgtgaca tgtttatttt ctcctgttaa
     4381 attgtcaggc cagaacggcc agttttcacg gggttcagat acttatttag ttcaagagct
     4441 gaactgaaca cgctctaaga actgtgcaaa atagataagt tggcttgttt gtaaacaaac
     4501 ttctccaact tcctaatttt gctttgttct ttacgagctt tgtatcttga ttttaccaaa
     4561 cgtaagcgat aacctcacca ccaacattct tttggcgtgc ttctttatca gtaagcaaac
     4621 cttcagaagt tgaaaggatg gcaattccaa gtccgttaag aacttttgga aggtcttcac
     4681 gttttttgta gacacgaagt cctggtttag aaacacgttt caagttagtg ataactttct
     4741 caccatttgg tccgtattta agaaatacac ggatgacgcc ttgtttgtca tcttcaatga
     4801 tttcaacgtt ttttacaaaa ccttcgcgtt taaggatttc agcaatccct tttttgatgt
     4861 ttgatgcagg tacttcaagt acttcgtgtt tagcttggtt agcattacga atacgagtta
     4921 ggaagtctgc gattgggtca gtcataacca ttttgttttt ctcctcttac tagtagtttg
     4981 caagttgcac ttgctagtta atacatgata caaagagcgt aacagcacga gcaaaaatag
     5041 acaatttaat gcagaagcaa tgctccaaag caaattggtc ttttttgcca aagctgtagc
     5101 tcgtgttcaa attggcaagc ccaattgaac ccgggctaaa ctctgtgtga aaaagataaa
     5161 ctttcctaga aactttagtt tcttcgtcaa gtttcctatt tttacttgga gttttgacgc
     5221 tcttgatatc ttaaattacc aagatgcttt tgttacacca ggaatttgac ctttatgcgc
     5281 aagttcgcga aaagcgatac gactcagacc aaatttgcgg taaactgaat gtgggcgccc
     5341 cgtaactcta caacgattat gtaaacgagt cggtgaggcg ttgcgaggta atttagataa
     5401 accttcgtag tcccctgccg cctttaatgc agcacgtttt tcagcataac ggtcaacaat
     5461 tttttggcgt ttagcctctc tagctaccat tgatttttta gccattagat ttacctccta
     5521 tattattttg caaaaggcat tccaaggcct gtaagcaatg cacgtgactc ttcgtcagtg
     5581 ttagcagttg ttacgataac gatgtcaaga ccacgagttt tgtcaacgtc atcgaagttg
     5641 atttctggga agattaattg ttctttcaca ccaagtgtgt agttcccgcg tccatcaaat
     5701 gattttgttg gaacaccgtg gaagtcacgt acacgtggaa gtgaaactga taccaattta
     5761 tccaagaatt cgtacatacg ttcaccacga agggtaactt ttgcaccgat cgcaacacct
     5821 tcacgaagac ggaagccggc gattgatttt ttagctttag tgataagtgg tttttgacct
     5881 gagataagtg ccaattcttc agcagctttt tcaaggcttt tagcgtttga tacagcttca
     5941 ccaacaccca tgttcaaaac aatcttatct actttaggca cagccatcac tgatgagtag
     6001 ttgaattgtt ctgtcaaagc aggaactact tcattaagat atttttcttt taaacgattt
     6061 gccattatac ttctcctttc cttcgtgatt aatcaagcac ttcgcctgat tttttgttgt
     6121 agcgaacttt tttaccgtct acaaatttgt aaccaacacg accagctaca ccatttttgt
     6181 ccaaaacttg aacgtttgat acgtggatag ctgcttcttt ctcgatgata ccaccttgag
     6241 gaagctcgtt agttggacgt tggtgtttct taacaatgtt aacaccttca acgataactt
     6301 tgtttacttt tggaagggca gtaaggacaa cagcttctgt tcccttatct ttaccagcga
     6361 ttacgcgaac tttgtcgcct ttttttacaa acattaggtt tctccttgat ttttcttacg
     6421 cccataaggg caccctagct tgaagctagg ggactagttt gttcctaaaa attaaagtac
     6481 ttctggagca agtgacacga tcttcatgaa gccaccttca cgcaattcac gtgcaactgg
     6541 gccaaagata cgtgttccgc gaggagtttt gtcttcacgg atgataactg ctgcgttttc
     6601 gtcaaatttg atgtatgaac catcagcacg acgagcacct gatttagtac gaacgataac
     6661 tgctttaaca acgtcacctt ttttaaccgc accaccagga gtagcttgtt ttacagatgc
     6721 cacgataaca tcaccgatgt ttgcaaattt acgtcctgaa ccaccaagaa ctttgatagt
     6781 caagatttcg cgagcaccgc tgttgtctgc gactttcaaa cgagtttctg tttgaatcat
     6841 ttcagttttc tcctttcagg tttgattaga tgatgaccgc ttcttcaaca acttctacaa
     6901 gacggaaacg ttttgtagct gaaagcgggc gagtttccat gatacgtacg atatcgcctt
     6961 ctttggcaac attgttttca tcatgagctt tgtatttttt agagtagtta atacgtttac
     7021 catagactgg gtggttacgt tttgtttcaa ctacaactgt gattgtcttg tccattttgt
     7081 cagatacaac acgtccaaca agaactttac gattattgcg ttccattgaa atttctcctt
     7141 ccctagtcta ttatttcgct tcagattgaa ctgttttgat acgagcgatt tgttttttaa
     7201 cttctttcaa gcgagctgtt tgttccaatt gaccagtagc agcttggaaa cgaagttcaa
     7261 acaattcttt tttcaattcg ttttcgcgct tcgcgagttc ttcttgagaa agaccacgaa
     7321 gttctttaac aaattctttt acttcattaa gtttcatgcc ttctccttat tctgcttcac
     7381 gttttacgaa tttacattta actggcaatt tgtggctagc aagtcgaagc gcttcacgtg
     7441 caatctcttc agatacacca gcgatttcga acatcacttt accacgttta actggtgcta
     7501 cccaaccttc aggtgcccct ttaccagatc ccatacgcac accgatagct ttagcagtgt
     7561 atgatttgtg tgggaagatt ttaatccaaa ctttaccacc acgtttcatg taacgagtca
     7621 tggcgatacg agcagcttcg atttggcggt tagtgatcca gtggctagtt gtagcttgaa
     7681 gaccgtattc accgaatgct acttcttttc caccttttgc ttcaccgcgc atttttccac
     7741 ggaactcacg acggtgttta acacgtttag gtactaacat tggttattta cctcctttag
     7801 tgtttttacg agctggaaga acttcaccac ggtagatcca tactttaaca ccaagtttac
     7861 cgtatgtagt atctgcttct tcccaagcgt aatcgatatc tgcacgaagt gtgtgaagcg
     7921 gaacagttcc ttcagagtat ccttcagcac gggcgatatc tgcaccgttc aaacgacctg
     7981 atacttgagt tttgattcct ttagctccag cacgcattgc acgttggatt gcttgttttt
     8041 gtgcacgacg gaaagcaaca cgttgctcca attgacgagc aattccttca cctacaaggt
     8101 gagcatccaa atcaggttgt ttgatttcga tgatgttgat gtgtacttgt tttccagtca
     8161 atttgttaag ttttgcacgg agtgcatcaa cgttagcacc acctttaccg ataaccatac
     8221 ctggtttagc agtgtgaagt gaaacgttaa ctttgtttac tgcgcgttcg atttcaatag
     8281 ttgaaactgc tgcgtcagca agttcttttt gaacgaattt acggattgca agatcttcat
     8341 gaaggtaatc cgcgtattct ttttcagcat accatttggc atcccaatca cggatgatgc
     8401 cgacacgcat accaattgga tgtacttttt gacccacgat tttacctcct tatttttctg
     8461 caacagctac agtgatgtga gctgtacgtt tgttgattgg tgaagctgaa cctttcgcac
     8521 gtggacggaa acgtttcata gttggtcctt cgtttgcgaa tgcttcagat actaccaaat
     8581 tagctttatc caaaccaaag ttgttttcag cgttagctac agctgagttc aaaactttca
     8641 agatgatttc agcagctttg tttggagtga atgtcaagat tgcgattgca tcggctacgc
     8701 ttttaccacg gatgttatca agaacaagac gtgatttacg aggtgaaaca cgtactgtac
     8761 gagccattgc tttagctgaa gtaatttctg ccatttatgt tctccttatt ttctacgtgt
     8821 tttcttgtcg tctgcagcgt gacctttgta agtacgagtt ggtgcaaatt caccaagttt
     8881 gtggcctacc atgtcttctt ggatgtaaac aggtacgtgt ttacgtccgt cataaactgc
     8941 aatagtgtaa ccaatgaaac ttgggaagat cgttgaacga cgtgaccaag ttttaataac
     9001 ttttttcttt tcgtcgttag cttgagcttc aacttttttc atcaaatgct catcgacgaa
     9061 aggtcctttt ttaagactgc gtcccatttt tatattttct cctttaaatg ttgtaccaca
     9121 gcggcttgca ctcctatgga gcgctaccga gctggcggat ttactagttg cttaagcgac
     9181 tagtttaata ttatttctcg ttgcgacgac gaacgataag tttgtcagat ttcgctttct
     9241 tgttacgagt tttaagacca agagcaggtt tgccccatgg agtagatggt gctttacgac
     9301 caactggtgc tttaccttca ccaccaccgt gtgggtgatc gttagggttc attacagaac
     9361 cacgaactgt tgggcggata cctttccaac ggctacgtcc tgctttacca aggtttacaa
     9421 gtccatgttg ttcgtttccg acaacaccaa ctgtagcacg gcaagttcca agaatcatac
     9481 gaacttcacc tgattgaaga cgaacaagaa catatttacc ttcagaaccc aatacttgag
     9541 cagatgcacc agcagcacgt accaattcac caccacgacc tggtttcaac tcgatgttgt
     9601 gaatcaaagt accaactggg atgttagcaa gtggaagagc gtttccgact ttgatatctg
     9661 cttctggacc tgaaacgata cgttgaccta cttcaagacc ttttggagcg atgatgtatg
     9721 ctttcacacc gtcagtgtag tgtacaagag cgatgtttgc agaacggttt ggatcgtact
     9781 cgattgtttt aacaactgct tcaacgttgt ctttattacg tttgaagtca accaaacggt
     9841 aaaaacgttt gtgtccacca ccttggtgac gaactgtgat acgaccgttg ttgttacgac
     9901 cagccttgct cttcaatgca acaagcaatg atttttcagg agtgcttgtt gtgatttcag
     9961 cgaaatccaa agaagtcata ttacggcgac cgtttgttgt tggtttataa acacgaattc
    10021 ccacgatatt tcctccttag attattcagc ttcagcagca aacaactcga ttgctttaga
    10081 atcagctgta agtgtgatga tagctttttt agttttgtta gtaaaaccag tgtaacgtcc
    10141 aacacgttta gcttttggtt ttacgttgat tgtgttaaca ttggcaactt taacaccttc
    10201 gaaagcagct tcaacagctt gcttgatcaa aagtttgtgt gcacgagtgt caacttcaaa
    10261 tacatatttt cctgcttcaa gttgagccat tgagctttca gtgatgacag gttttttgat
    10321 aacatcatac aaattcatta tgcaagaacc tcctcgattt tagagatagc tgcttgtgtg
    10381 acaagaagtt tgtcgctatt tgcgatgtca agaacacttg cagttgtagc agttgcaact
    10441 ttcacgtttg gaaggttacg agctgaaaga gctgcgaatt catttccttc ttcaaggata
    10501 acaagaactt tagaatcgat gctcaatgct gcaagaactt ttgcaaattc agcagttttt
    10561 ggagctgtaa atgaaagagc gtctacagct acgaatttgt tttcagcaac tttttcagag
    10621 taaactgatt taagagctag gcgacgaact ttttgtggaa gtttgtagcc gtatgaacgt
    10681 ggagttggtc cgaagacaac accaccacca cgccattgtg gtgagcggat agaaccttga
    10741 cgagcacgtc cagttccttt ttgacgccat ggtttgcgtc caccacctga tactgcagag
    10801 cggtttttaa cagcgtgtgt tccttgacga aggcttgcgc gttggctgat gattacatca
    10861 aacacaactg attcatttgg ttcgatacca aatactgcat cgctaagaac aacttggcca
    10921 gcttctttac cagtttggtc aaataatgtt acgtttgcca ttgtgactga tttccccttt
    10981 ctttattatt taccagcttt aactgctgat ttgatagtga taagagattt cttagcacct
    11041 ggtacgttac ctttgataag gataacgttc ttttctggaa caacttgtac aacttcaagg
    11101 ttttgaattg ttacgcggtc gccacccata cgccctgcaa ggtttttacc tttgaatacg
    11161 cggttaggtg caacaggtcc catagaacct ggacgacggt ggtaacgaga accgtgagcc
    11221 attggtccac gtgattgtcc gtggcgtttg ataacacctt ggaaaccttt acctttagaa
    11281 gtaccagtta cgtcaacaac gtctccagct gcgaatgttt caactgtaat ttcagcacca
    11341 acttccaagc cttcaacgtt tttgaattca cgaatgaagc gcttaggagc cgtgttagct
    11401 ttcgctacat gtcctttagc aggtttgttg ctcaatactt cgcgtttgtc atcgaaacca
    11461 acttggatag cgttgtatcc gtctgtttca acagttttaa cttgaagaac aacgtttgga
    11521 gttgcttcaa taactgttac agggatcaat tcgccagctt cagtgaagat ttgagtcatt
    11581 cccacttttt tccctaagat tccttttgtc atgagaaaat agttcctttt ctatattttt
    11641 attcaaaaag tttttaacga gcgtttttta tgctcaagtc taagatacaa agggcgtcaa
    11701 actcaacgtg aaaataggaa actgacacag tgtctagcag acgcgaggaa gtttatcttt
    11761 ttcacaccga gtttagcccg tgttcaatta gattgaaaca ccagcctctg ctcttttata
    11821 ttttagatta aagtttgatt tctacgttta caccacttgg aagatccaat ttcatcaagg
    11881 catcaactgt tttttgagtt gggttaacga tatcgatcaa acgtttgtgt gtacgcattt
    11941 caaattgttc gcgagagtct ttgtatttgt gagtcgcacg aatgattgtg tagaggctac
    12001 gttcagttgg aagtgggatt ggacccgcaa cttgtgcacc tgtacgagta gctgattcta
    12061 cgatttttgc agccgctgtg tcaagcgtac ggtgttcgta agctttcaaa cggatacgga
    12121 tttttttgtt tgccatcttt ttctcctttt cgtctattta agataatagg ctagctccac
    12181 aagaaaaccg acgcggtgtt gcgtggcaat gcaaccgagc gtgtcgcaac ctcttgcatc
    12241 aaaactaagg ctgtaattta cagcaccata atagaataac acaaatcccc tgcgattgca
    12301 agggatttgt taggtttttt cgttttttgg ggatgaaact gttttcctac tttttctttc
    12361 taaaattagg gattttcttc tatatttctt ctatacataa gttaagtcct cttgacatca
    12421 aatttatctt ctgattggtc cactaagata tccgccttag actcagtttc tttatagtat
    12481 cgcagatact gctcccgtct catctgatgg ctagccaata taaaggatgc atcgcgattt
    12541 ctcacagtcg tatctctagc aaggcgtcgc tttaattcgg tctcctcatc cgtgtagaaa
    12601 cagatggttt tttcaaagag ttccttgggt agaaagccaa cagacatccc ttcgacaatc
    12661 aaaattggtt tggctccaga caagacctca ctagccttcc aaggttcttc aattgtcaag
    12721 acatccatac ccgcctgcaa ggcaaggata tctctctgca aactctccag ttcatgcgcc
    12781 actggcagac tggctgtcac cttttgattc ggcgcgtcct tgggtactac caaatagcgt
    12841 ccggaggtga tatagggatc tgtttctagc aaatttactg tagtagaatc taaagcttgg
    12901 tacaattcct gtgcaaaggt tgatttacct gaagctccat gaccgtatat tcccagtgtc
    12961 ctgactttcc ccgtctcgat ctctgagact agttggtcta ctaagtcttt tttcttcatt
    13021 ctctcttcac ctgttcatag atttcttttc cgtcattgag cttgtcccaa atcactactt
    13081 gcccactttt gagcgatttt tgcacatcga taattcgttg gttagacgaa cctcgaaact
    13141 ggagcataag atttctctta gttcgatcat atcttccatc gacaagaatg tcaatcagtg
    13201 acaagagttc cagtttatct ggagtttcca acatcatttc ttcccaagtg tagcccgtcc
    13261 aggaccagat gtccttgtct ggcaattcct tccgaatccg cttaacaagt ggcaagagaa
    13321 tcccagtatt aagaaaaggc tcccctccta gtaaggttaa cccctgaaca taaggctggg
    13381 caagatctgc cataatctgt tcttccaact cagctgtata gggaatgcct gcattgaagg
    13441 accaggtcgc tacattataa cacccctcgc agtgaaacat acagcctgct acatagagag
    13501 agttacgcac gccttcgcca tcaacaaagt taaaggcctt gtagtcaatg atacgacctt
    13561 gactaagttc ctcgcttttc cattcttgtg gttttggatt attcattcgc tacctctatc
    13621 caataacgct cgactccatt gcgagcatcc tcaaatattc caccatttgc tagaatgact
    13681 gctctgctag caggattatt cacgctacag gtcaccagag ctttcttgat gttcttttcc
    13741 ttagcaactt gcaagccctg acggagagtc tcttttgcat aacccttgcc tctttcagat
    13801 ggacggatgg agtagccaat gtggccagca taattcagta acccctcatt cagtctcaat
    13861 cgcagattca aaaatcctag agcacaacct gattcaccaa acaatacgaa ctgtatcgat
    13921 ggaacgcgat tttcaggtag ccatattccc atttctttat tacggttacc ttctagccac
    13981 tcctcgtaag aaaaatcctc aacatcccaa aatcctccat catgggctga ttggctcttt
    14041 tcaaactctg tcatcatatc taaaacagct ttcttatccg ctaatcttgg tctgcgtaat
    14101 tccatcctta cctcccacta tgagaaaagc gagagctggc tctcactttt attttttctt
    14161 gttcttgttt aaaaattgtt ctctgaagct aagcaagcgt tctttcttac cagctccacc
    14221 tttagagtgt ttctcgtgat aacgggtcac ttgtgcgcgc cccttatcgt ctagttgata
    14281 ttttcccatt tcatttcttt ctaatttgtt acttgatgcc cagctatttt aatcgttgaa
    14341 ccattcatat gtttgacacg cgcagcgatt tccttgtgac gcccgttgac catcggtctt
    14401 gcttgaggat tacctaggta gccacaagtt cgcttcacca catctactgt tttagggtcg
    14461 ctattgccac agtttggaca agcaaaccct ctctcagttg gttcaaaatc cccttcaaag
    14521 tcacacttgt agcaacggtc aatcggagta ttggtgccta gatagcctac acggtcataa
    14581 gcataatccc agacagcttc caaggccttt ggattttgct gaaggactgg atactcacaa
    14641 taatggatga aaccacctga cgcacctgct tctggataga ctttctcaaa gtccaatttt
    14701 tcaaacggtg ttggattttt acgaacatcg tagtggaaag agttggtgta gtattccttg
    14761 tctgtgatat caggaataga gccaaacttg tctatatcta gtcggcagaa acggtctgtc
    14821 agactttcgg atggtgttga gtagatagag aaatggtagc catattggtc tgaccactct
    14881 tctacacggc gtttcatatc gtgaatgatg tctagcgtga attccttagc atctggatta
    14941 ctttcccagc tgttaccaaa gaaaactgtc gctacttcat acaagccgat atagcccagc
    15001 gaaacggtcg cacgacaatt cttaaagagc tggtcaacac tttcttcttt acctagacga
    15061 tggccaaaag caccgtactg ataaaggata ggagcattcg ctggtgtcgc ctctttagtg
    15121 cgttcgacac ggtaaacaag agcatcttct gcgatattca ttcgctcgtt gaagatttcc
    15181 cagaacttat tcatatcacc ttcagactca agagcaatac gaggcagatt aaccgtcaca
    15241 acacccagat tcatgcgacc tgaattgact tctacaccat tttcatcctt ccatccttgg
    15301 aggaaagaac ggcaacccat aggcaccttg aaggatcctg tcaagtcaac aatcttatcg
    15361 taagataaaa catctggata catccgtttg gttgcacact caagagccaa ctgcttgatg
    15421 tcgtagttgg gagttccttc ctctaaatta aggcctcttt tcagagtgaa aataagttta
    15481 ggaaagatag ccgtacggtg ttctgaacca agacccttga tgcgaatgtt taaaatagct
    15541 ttttgaattt ctcgttcaaa acgactggtt cccagaccaa aacctagcga agtaaaaggt
    15601 gtttgtccat ttgaagtgaa gagagtattg atttcatact caagagattg catggcatcg
    15661 tagatgtcct tttgcgtttt cttccaagcg taatcttccc gtttttcagg caatacccac
    15721 tcttctgcat ctttgagatg tttttgatag ttcttctctg cataaggcgc caaaatttca
    15781 tcgatacggt cagctgaaca gccaccgtac tggctagaag caacgttggc aatgatttga
    15841 gaaatctgtg ctgtcgcagt ctggatagac ttgggactct ctacctctgc atttccaatc
    15901 ttaaaaccat tttccaacat acccttaaaa tcaatcaaac agcagttggt cataggggta
    15961 tagggactgt agtccaaatc gtgatagtgg atatccccct tttggtgggc attggctacg
    16021 tgcttaggaa gcatttgcag tccgattgat ttcccaacaa tccctgctgt caaatcacgc
    16081 tgagtgttaa agacatcact gtctttatta gcattttcat tgacaactgt ctggtctttg
    16141 ttgagaagtt tatgaatact aaagttgata tccgtcgctt ttgagcgctc aaaatccctc
    16201 tgtgtccgat aagtaatata ctcctcagcc agcgcatatt ctttggcttc aaggagttca
    16261 tgttctacga tattttgaat ttcgtaaatc ttaattccct gtggaaagcg actatgaatt
    16321 tctgtaataa ttctctcagt cagatcattg aggcgttttt caaccagggg tgtcacatcc
    16381 ataaccttgt cagctgcctt gtggagagcc ttgtcaatct tgtccacatc aaatacaaca
    16441 cgtctcccat ctcgtttctc gacgaagaga gttggcaaaa ttgtaatttt ttcttctagt
    16501 gcaatcatat gcatgctctt ttctaaaatg ttctttctaa aaagtattat accactctaa
    16561 aaagaaaaat caatatcttg tgttaaaaat cctaaaattt ttaaaaatac acaagatatt
    16621 gatttggtta tttaatttta tagactttaa actctgagta atcagtcttt acttgttggt
    16681 aattttcttt taaaagtgtt tccacttcag accagactac caccttgtca tttaccacaa
    16741 tcaccttagg ttgtttttct ttcaagtcat taagtaactt attacgattt tcctcagttg
    16801 ctgtatagtg caacggggac aaaatcgccg atggcgacaa gcgctcactc ttacgataaa
    16861 gagtcgcagt atcatcccat gcatagatag catcttccgt atttgtctcc tcttttacca
    16921 aatcagcaag acgattacgt tcttgataag gtactggata aaggacaaac ttcatcaaaa
    16981 aaggaacaga caaaagatag acaaacccaa aaactagcag atatagattt cctttgaaga
    17041 aacgactcca taggctaact ggcgtttctc ttcggcttct tctagcacta cgccccctct
    17101 gttgactctt gatattggtt accaataaaa gtaggaaaac agggaagacg accatcaaat
    17161 aagaagcgtg cagaggctct tgtgaaaaga aaagtaaaat caaacctaaa attaccacaa
    17221 gacttgcagg aaccgaaatg acgtatagtt taaagggttt ggattgaaag agacctaaaa
    17281 agatacaaac cacaaatccc aaaccaaggg acaacaagcc ataaaaaaac atattctcta
    17341 aaattttaga agtaaaatca aagcgaatac tgtcaatagg ataacgaata ccactaatcg
    17401 catccccaaa acttcctgtt gcagcactat agtaggcagt tggataaaag acaagtgaaa
    17461 aacctaaagc cactgcaaga aactgataaa acccatgcgc aaagcgtcta tgcccaaggt
    17521 taaagaccaa caagcctaaa ctcactacag caataaacag gagtgatgat aagggagcaa
    17581 agaaaaatcc gcctgccaaa gctaacccaa tccgtacaaa tcccttatca tggcttggat
    17641 tgcttaggta agccgcaact aaactaaagg ctgcgaataa gaaaggaagc gctaaaagag
    17701 tcgcatagcc tccaccaaaa gcaagacctg taactagcat gtaaaaaata gtcaccagat
    17761 gtccagcttg gtctccttgc tctgtcaagg tgtccgctga tctaaaaagg aaaaatcctc
    17821 ctgctaccaa ggctaaccac tcaaagatgg caaagaaaaa tccgccctga ctcacgtaag
    17881 tcagcaaata ataaagcaaa ccttgacttc cataatagtc gctgtaaatc ttccctgtct
    17941 gatgaagcgc ccaacctgca taaaaatcct gcacttcttg tgcactcatt aagtcgagta
    18001 atagcggtac tcctagagtt atccccgtta caagcgtact ccatagtaaa attttcacca
    18061 aaggaagacg acttgattca cgatgatgcg attcttgctc gatttggtat tctagaggtt
    18121 cacgattctc cttatgaact tcttctactc taccatacac actcatatcg tttctcctgt
    18181 tcaatttatc tgtcttagta tatcaaaaag tcctaggatt gtcagcttgc gatggctgtt
    18241 tgagtgattt tttgcatagt ttcacaaagg tttcaatttc ccgaaagcgg tgtaaatgtg
    18301 tctgtttgaa ctcaatcata tcattcactt cttccttagt tagcatgaat tcccctcctc
    18361 tttctttcta ttcgtaaaag acaagaaaaa taggtcgggt ttgtatccta agttctagat
    18421 ttcttataag ataggggcga atagctgggc gatttctttt acaagctttt gataccagct
    18481 cgttttgata gaatgaggat agacttcctg agaatctgca aatatatttc gaaaatctcg
    18541 ggcgatttcc cttatagaag gagttttata gagtaagact gcattttcat aatggtgtac
    18601 caagctccgg tagtcgagat tgattgtccc caccaccgca aaatcttcgt ctaccaacat
    18661 ctgcttacta tgaataaaac ctggactata ctcataaatc cgaacaccag cagaaagaag
    18721 gtcgggataa gctcctctcg tgactaactg aatgaacttc ttatctggta tgtaaggggt
    18781 gataattcga acatcgaccc ctctcatagc tgcatttttg attgtctctg ttaaatcata
    18841 atctataatc aaataaggtg tcgtaatata gaccgattcc gttgcttgat tgattaaact
    18901 ctgataaact tttttcccta cctgcgctcg aaaaattggc ttgggtccac ttccgtatgg
    18961 aatggttaaa ccgtcactcg ggatagaatg attttctaaa tgatattgat caaaatcact
    19021 aatttctcct cgattaatgt accaagtggt caaaaataag cgtgtcaggg cttttactgc
    19081 tagtccgtct aagcgaattc cactatcctt ccaataacca aatctctcga cgtggttaat
    19141 gtactcatct gccagattga ccccaccagt ataggctatc tgaccatcaa caatcaatat
    19201 ttttctatga tctctgttat tataagccac tgtcaaacga ggaataactt tattgaattt
    19261 atgggcctcg atgcccagct gacgaagtcg atgtgcataa tctcctgtta aagtagccat
    19321 acagccgata tcatcataga gcatcttaac ctctacaccc tgagctacct tttgctctag
    19381 tatatctagt atgcgattcc acatcaaacc ttcttctata atgtaatatt ccaagaaaat
    19441 aaatttctca gcctttttaa gatcttctac catctttttc cacatagctt ctccgttagg
    19501 aaaaaatgta gaggcagttt gatcatagat gtcagcattg gtatccatac tcaataagga
    19561 cttgatgact ccataagctg ccttgtcact ttcttttaat ttctctttta gtagctggct
    19621 atttgcttct tggaaatgca tagagcctag cttcttcagt tgtttgattt cttttttgga
    19681 caatcgcctt tcaccaaaca tcagatagag taagggacca aatactggca caaaggctac
    19741 taacaaccag gttaccttat tctcaggagt cgtattacgg ttgactatcg aaatgattgt
    19801 gatgatactc agtagaatga ggacagtaat ccataaaatg ggagccatgc gtcctaaata
    19861 aagaaagaga ccaaaaacaa gacaaagttc aagtagcata atcgaaagac taaagccata
    19921 cttggacatc aataattgaa atttcctata tttcatatcc cctccttgat agtttgaatg
    19981 ctaaaaacaa gtaattgata ctagtataac gcaggtattc ggtagaaggc aagtatttat
    20041 gattattttc taggtaaaag taaaaaggct gaaaaatcca gccctttcta cttgcatagt
    20101 cttatttagt taaaacgatt tggtccgatg ggtctctatc catatcatct acgacgattt
    20161 gattaccgtt tacagtgtaa attttgacat catcgccaat aatcatgcgt tgatttgctg
    20221 aatcaaatgt aacctgtttg actttctgat ccccgtcaga ttcgagctca gtccatgtac
    20281 cagtcgttcc agttaccact aaagtgatac ggtctccgtc atcctgacca gtataagtcc
    20341 catcaatatc ggtcggttga gcaacaggtt ggttttgtac tggagccgct tgattttggt
    20401 tacctcctgc atttgcagta ttggtattct gttgagcagg ttgttgagca ggctgttgga
    20461 cctgctgctg agcaacaggc tgttgaacct gttgagctct ttgtgaacaa gctactaaga
    20521 gactaaaact tgctagagaa gcaagagaaa gtgtgaatct ttttaatttc atgatgaatt
    20581 tcctttctgc taccaattta gagaaatttt ctctaaccag caattcccct agtataacaa
    20641 gttcaaacaa gggggtcaat ttatctgctc acggtccagc aggtagcccc gtacttctga
    20701 gataaaatag agagaccctg taacgaacag caagtcttga gcgtctgccc tttcttcgaa
    20761 actgctgata aattctcggc aagaagaaac tatatcgtaa cctgtcacat ccctttcgtc
    20821 caaagccccc tgatagtcaa agccggtcac cttgagttcc acctgaggca atttttcagt
    20881 caggtaacct aacatccctt gataatcctt ccgtttcaag gctccaaaaa ggatttgagg
    20941 tcgatagcct tcctgctctt tttctttgat aaactcaacc aagcgagtca aggcagggag
    21001 gttatgagca ccatccaagt aaatctgtgg gcgaatacgc tccaagcgac cagcccaatg
    21061 ggtctgttcc aaggcctttc ttacagcctg ttcatcaaca gcttcctttc cttctcccat
    21121 aaaaagaaga aaagtttgca acgccaaggc tgcattctcc tgctgataga ctccttctaa
    21181 gcctatgttc agctgtgaaa aatttagtaa agagcttgaa aaatcaccat tcagcattaa
    21241 aaaatcttga cctgcctgat aaaggttaac agctaaagat tcggcttttt tctgacagac
    21301 aagcctagct tctggaggca atttcgcaat cactgccttc ttaccagcct tgaaaatacc
    21361 agctttctgc tctgcaattg cttctagact atcacccaag gtttcttgat ggtcaagccc
    21421 aatggaggtg atgacagcaa gctctccagt taccacattg gtcgtgtcaa gtaagccacc
    21481 aattcccact tctaataaaa ccaaatccac ctcttgctcc ctaaaataaa gaaaagcaac
    21541 caaggtcagc atttcaaaaa aggacaactg gtcaggagtt tgcagaagcg ttttctccat
    21601 ctccttgacc tgatcagcca aacggataaa gtctgcgtct gctataggtt gcccattaat
    21661 gcagattcgg tcattgatag agacgatatg aggggaggta aaggtcgcaa cttttttgcc
    21721 atgccccata aataattccc tcataaaagc aatggtagat cctttcccat tagtccctgt
    21781 tacgtggata atagggtaag actgctcagg atttcctaac aaatccaccg cttgctgtat
    21841 tcggcccaaa cctgatcgaa aattcaaacc aatccgacta tgaagccatt cttctacttc
    21901 aaacatacac atctccttga caaaagtcca atcaattatc gcattaaagt atggttacta
    21961 ataaaaacaa ggccaggatt ttcgtcccga cctcttacct ggttagctaa taactagcca
    22021 ctatgaatgt gaatatgggc taaaaacatc cactagatgt tccaactctt ccccatttct
    22081 gggagttggg ctgatacagt ctcccagact gtatcactcc tccataaagc tgttgaagac
    22141 ttcttcaatc atgttccatt cgtcttctga gtcttctggg attggttgca attcgccttc
    22201 tgttccatct tcattttcaa tgaatgagta agcttggatt tcaacttgtc cgtcttcgtc
    22261 ttcttctgcg ttaactggta ctagaagaac atagttttta ccaaattctt cttttccatc
    22321 aattgtcaaa aggatttcaa acaaggtttc atttccttgc tcatctacta gtgtgattag
    22381 ttcacgttct tcgtggtcgt ggttatgatc gtgtgacata gcctctcctt tatattaaaa
    22441 ttttctatct aaataatttt gtaaaatcag ctgagctgct aacttatcaa tgactttctt
    22501 gcgcttattg cgactgatat ctgcttgttc aatcaacatg cgctcagcag ccactgttgt
    22561 caagcgttca tcctgatagt ctaatggtaa accaaaaaac tcttctagct ttgctccgta
    22621 ggcttgacta gcttctacgc gcggtccact tgtattgttc atgtttttag gcaagcccac
    22681 tacaaatcgt tccaccttgt aagtatcaac caattcctta acgcggtcaa aaccaaattg
    22741 gccttgttct tcatttatct ggatgatttc aagcccttga gctgtaaaac caagcggatc
    22801 gctaatcgcc acccctaccg tttttgaacc gacgtccaat cccataattc tcataggtta
    22861 tagatcgact ccttgtcctt tgaggtagta gcgaaccaat tcctcaacga tttcatcacg
    22921 ctcatactta cggatttgat ttcgtgcatt attataacga ggaacgtagg cagggtctcc
    22981 actcaatacg taacctacga tttggttaat tgggttgtaa cccttatcgt tcaacgaagc
    23041 ataaacatct gtcaaagttt cgctaatttc ttttttattg gaatcgtcca atttaaaacg
    23101 tactgtttct tcagtaaatc ccattctaac accctctttc cttagaatag taccattata
    23161 gcataattcc ttaccttcta caattcaggc agtctattta tttggatttt ctattgttct
    23221 gtcgcgccat ttgccaatct atctgaaata tatttgcttg gttcattttt caaaagattt
    23281 tccaaaccaa tattcttcag atgttccaac tgggaagcct tcttgacatc cagaacttga
    23341 aaatcaaaac tagtcgttgt ttgaagttcc gttgcgctca atagttttgt ttcaagtttg
    23401 aaacctgcca atttacgagc ttcaatgata gacttatcct tctcctccgc ttcaagaaga
    23461 gctttttgag tttcctccac tccatgttgg tcaatataag tcttcaactc atcagagact
    23521 ggacgttttt gttggatagt tagactcaaa aaagtcttgt ccttgtatgt aatggtttgt
    23581 gtctgctggc taccatcatc tgacttgggc aagaccaaag tcttagttac aactgtattc
    23641 ttctcagcat tttcaataac tggcaatgcc gactgaagcg tatctttttc tgtttttgta
    23701 gctggtccag tttctttttt ctgtccgcaa ccaaccagga caaaaaggaa agctagacta
    23761 acaagaacta tttttttcat ttctttcttc tttctttttg aaattaaaat agaataagac
    23821 tgggaagtgc tcccagcctt gatgtttata gagctgcacg caaacgtgct tctgcatttt
    23881 ctacattacg gacagagcgt ggtaggaagg cacgaatatc gtcttccttg tagccaactt
    23941 gcaggcgttt ttcatctaca aggattgggc tctttaaaat tctcggtgtt tccataatca
    24001 gattgagaac ttcattgaca ctcaaatctt caatatccac tccaagggct ttggcatagc
    24061 gatttttaga cgaaacgatg ctggctattc cgttatctgt tttggttaga atatccagta
    24121 attcttctct cgtaattcct tctttaccaa ggttttgttc tttataactt aactggtggg
    24181 cattgagcca ggtttttgct tttttacagc tagtacaact tgagactgta taaattttaa
    24241 tcatgtacct acccctttcg ctacatgtta ctatcagttt agtctattat accataaaaa
    24301 acatccgact tgcgacctat ttttaat
//
LOCUS       DAVBCB010000030        24206 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVBCB010000030 DAVBCB010000000
VERSION     DAVBCB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 24206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24206
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.030"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..289
                     /locus_tag="VQU28_001185"
     CDS             <1..289
                     /locus_tag="VQU28_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476546.1"
                     /translation="MMTSDFFEAWFQKFLLPTLTTPSAIIMDNARFHRMGKLELLCEE
                     FGHKLLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCCNTFYEALLSCSCFN"
     gene            complement(496..2754)
                     /locus_tag="VQU28_001186"
     CDS             complement(496..2754)
                     /locus_tag="VQU28_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2476547.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2983..3213)
                     /locus_tag="VQU28_001187"
     CDS             complement(2983..3213)
                     /locus_tag="VQU28_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HET2476548.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3403..4086
                     /locus_tag="VQU28_001188"
     CDS             3403..4086
                     /locus_tag="VQU28_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2476549.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSEIVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAVKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            4086..4820
                     /locus_tag="VQU28_001189"
     CDS             4086..4820
                     /locus_tag="VQU28_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2476550.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDIAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4974..5831
                     /locus_tag="VQU28_001190"
     CDS             4974..5831
                     /locus_tag="VQU28_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HET2476551.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGYRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5973..6845
                     /locus_tag="VQU28_001191"
     CDS             5973..6845
                     /locus_tag="VQU28_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HET2476552.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLMTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQERVSKDKLV"
     gene            7127..7810
                     /gene="rpiA"
                     /locus_tag="VQU28_001192"
     CDS             7127..7810
                     /gene="rpiA"
                     /locus_tag="VQU28_001192"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HET2476553.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7824..9035
                     /locus_tag="VQU28_001193"
     CDS             7824..9035
                     /locus_tag="VQU28_001193"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HET2476554.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            9037..9579
                     /locus_tag="VQU28_001194"
     CDS             9037..9579
                     /locus_tag="VQU28_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2476555.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVHYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9595..10404
                     /locus_tag="VQU28_001195"
     CDS             9595..10404
                     /locus_tag="VQU28_001195"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2476556.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10880..11860
                     /locus_tag="VQU28_001196"
                     /pseudo
     CDS             10880..11860
                     /locus_tag="VQU28_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855254.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
     gene            11885..12397
                     /locus_tag="VQU28_001197"
     CDS             11885..12397
                     /locus_tag="VQU28_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HET2476557.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPISAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            13044..13754
                     /gene="deoD"
                     /locus_tag="VQU28_001198"
     CDS             13044..13754
                     /gene="deoD"
                     /locus_tag="VQU28_001198"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HET2476558.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13777..14301)
                     /locus_tag="VQU28_001199"
     CDS             complement(13777..14301)
                     /locus_tag="VQU28_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HET2476559.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     CEWMLTEIKNFLTKHKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAADCPERFEWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(14354..14590)
                     /locus_tag="VQU28_001200"
     CDS             complement(14354..14590)
                     /locus_tag="VQU28_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HET2476560.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14658..15578)
                     /locus_tag="VQU28_001201"
     CDS             complement(14658..15578)
                     /locus_tag="VQU28_001201"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HET2476561.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEKKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15768..16172
                     /locus_tag="VQU28_001202"
     CDS             15768..16172
                     /locus_tag="VQU28_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476562.1"
                     /translation="MKILKRYILELCFILSFALPFIKGTNADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            16388..16969
                     /locus_tag="VQU28_001203"
     CDS             16388..16969
                     /locus_tag="VQU28_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2476563.1"
                     /translation="MYYAENPDAAHDIHELRVDLLGQKMTFLTDAGVFSKKMVDFGSQ
                     LLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16966..18243
                     /locus_tag="VQU28_001204"
     CDS             16966..18243
                     /locus_tag="VQU28_001204"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HET2476564.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLVEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIRGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVHQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            18261..18923
                     /gene="deoC"
                     /locus_tag="VQU28_001205"
     CDS             18261..18923
                     /gene="deoC"
                     /locus_tag="VQU28_001205"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HET2476565.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18910..19299
                     /locus_tag="VQU28_001206"
     CDS             18910..19299
                     /locus_tag="VQU28_001206"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2476566.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGSVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMAEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19389..20441
                     /locus_tag="VQU28_001207"
     CDS             19389..20441
                     /locus_tag="VQU28_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HET2476567.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALNNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20585..22120
                     /locus_tag="VQU28_001208"
     CDS             20585..22120
                     /locus_tag="VQU28_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476568.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            22113..23171
                     /locus_tag="VQU28_001209"
     CDS             22113..23171
                     /locus_tag="VQU28_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476569.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            23174..24130
                     /locus_tag="VQU28_001210"
     CDS             23174..24130
                     /locus_tag="VQU28_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476570.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         7033 a         4379 c         5503 g         7291 t
ORIGIN      
        1 gatgatgacg agcgactttt ttgaagcatg gtttcagaag tttctcttac caacattaac
       61 cacaccatcg gctattatta tggataatgc aagattccat agaatgggga agctagaact
      121 tttatgcgaa gagtttgggc ataaactttt acctcttcct ccctactcac ctgagtacaa
      181 tcctattgag aaaacatggg ctcatatcaa aaagcacctc aaaaaggtat taccaagttg
      241 ttgcaatacc ttttacgagg ctcttttgtc ctgctcttgt ttcaattgac tatagcagat
      301 tgaaataaga tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag
      361 caattgtttc gtactatttc agattcagtc tactatatat cataatggac aaagggcaag
      421 ctgtcaagaa attacagtta ttttagtaaa aaaagaagca gaaaaattta attttctact
      481 ccttttctat aggatttatt tttctttttc agaactttta acttcagctt ttttggcaga
      541 tttaatctga atgtttccct tgctagtcat aactgctttg agatcttttt cgcttggatt
      601 ttcaaggtag tagtcagtga ttgtgtcctc aatatagtct tgaatagtcc gacgaagtgg
      661 gcgtgctccc atttttggat cataacctag gtcaaccaac ttttccttga ccttgtcagt
      721 tacatccaaa cgaatgttgt tgctagagag gcgcttgtta acatctgcta gcatgagctc
      781 gacaatctga aggaggttat ccttgctgag agccttaaat tcgataatgc catcaaaacg
      841 gttcataaac tctgggctaa agaagttacc gagttcaccg aggacagaat tggtacgtcc
      901 ttctctagca gcaccaaatc caacgctagc ttcggtcttt cctgtacctg catttgaggt
      961 catgataatg atggcatcct tgaagctaac ggtgcgtcct tgcccgtctg tcaaacgacc
     1021 atcgtccaag acttgaagaa acatgtgcat aacatctggg tgagcttttt ccacttcatc
     1081 gagaaggatg agagaatatg gattgtggcg aactttttca gttaattgac cagcctcatc
     1141 atagccaaca taacctggag gagcgccgac caacttagcc acactatgtt tttccatgta
     1201 ttcactcata tcaaagcgaa tcatactatc agcagaacca aaaagttcga tagccagttg
     1261 tttggaaagt tctgtcttac cgacaccagt tggcccaacg aagaggaagc ttccgattgg
     1321 gcggttaggg gtaccaagtc cgacacgatt acggcgaata gccttggcaa tcttatcgac
     1381 tgcatcatct tggccaataa catgagactt gagatcttcg gctagatgga tgagttgaga
     1441 ttgttctttc tctttcaaat caccaacagg gatattggtt ttctgctcga taatgtgctc
     1501 aatagttttc tcgctgatga taggagtatc ctggtctgtg atctttttct tttgcatttc
     1561 cttatacttg gcaatctggt cgcggaagta ggccgccttc tcaaaatctt cttctcgtgt
     1621 agcttgagac ttgagatttt cagcctcaat caagcgctga tcaattactt taggatccac
     1681 aaaattcaag gtcaagttca tcttagaacc agcttcatct aggaggtcaa tggccttgtc
     1741 aggcaagaag cgatcttgga tgtagcgatt ggaaagagtt gcagctgctt caatcgcagc
     1801 atctgtatat tgaacgtggt ggtaatcttc gtatttcttt tgaatccctt tgagaatagt
     1861 gattgtttcg tccaccgttg gttcatcgac tttaacaggc tgcatacgac gctcgagggc
     1921 agcatccttt tcaatgatac ggtattcatt gagggtagta gcaccgacta gttgcagttc
     1981 tccacgagca agggctggct tgaggatatt tcctgcgtcc atattaccat cactcgcaga
     2041 accagcacca acaatttcat ggatttcatc gataaagagg atgatgtctt cacgtttgcg
     2101 aatttcttcc atgagttttt gcatgcgttc ttcaaattgt cctcgaatcc ccgttccttg
     2161 aactaagcta accacatcca gacggatgac ttgtttacct tggagtttat gtggcacatc
     2221 gccatcgaca attttctgag ctagaccttc gacaacggcc gtttttccga caccaggttc
     2281 accgataagg acaggattat tcttggttct acgattgaga atctcgatga cacggataat
     2341 ctcatcgtcg cgcccaataa cggggtcaat gtctccacga cgggcaattt cagttacatt
     2401 aataccaaat tcttccagca ggcctttttc ttggctaggt ggcggagttt gagcagatcc
     2461 acgattttgg gaaccataac cgccgtttcc accgtaacct ccacctgatt gggttggggg
     2521 aataggagga gtattgctag aaggtctgaa attgtttaga tcattgaaga aatcaccaaa
     2581 gggatcgaag tcacgattgt tcagatccgt catacctttg aagaggctat tgttaggatc
     2641 tgttttgata atcttatagc agttttgaca gaggtcaatt tgtttttgtt ttccattgag
     2701 attggtgtaa agatgaattg ttgagtcgtt aattttacag ttttgacaaa gcatacatct
     2761 acctcatttc tttctttagc ctgacttgtt taaaggtcaa aaatagtcaa attctatact
     2821 ctcattataa caggattggg gtgtaaagca agtaaaaaga ttgaagcttt gagaagttgt
     2881 tacaatgaaa atttttgtga atttggacta taaaaaaatc ctatttgtga gacaaaaagg
     2941 attttactta gacatgcagg gacaatccca gcttgtttta gattagtaga gaagttcgta
     3001 aatttccatt gcgattaagt caatgctatc aaacgtatac gtttcgcgat cttctacgtc
     3061 acgaagggta aagattgggc cgttttcttc gtcgtggctg tacgcaactt ctgcaacaac
     3121 aactccttcg cgttcaaaat tacgttttaa atttccgcca tcgtttgcca ttgcttcaag
     3181 gcggttgatg attctaacca aatgtgattc cattttgatt ctccttcatt tcttatcgtt
     3241 actattttac cataaaggag gccaacttga ctagaaaaaa ctaagatttc tcgctaattc
     3301 tgctagttta aagattttta aataaattgg ataaaaaatt taaattttaa ttcaaaacat
     3361 gttataatca tacagtattc tattttagaa agcagtgtga ctatgaattt ttctttttta
     3421 cctaagtatt taccttattt taactatggg gctgttgtga cgattcttat ttctatctgt
     3481 gttatctttt tgggaactat tttgggtgtt gtcttggctt ttgggcaacg ttcaaagttt
     3541 aaaccgcttg tttggttggc caacttgtac gtttggattt tccgtgggac accgatgatg
     3601 gttcaaatta tgattgcctt tgctcttatg catatcaatg ctccgactat tcagattgga
     3661 attttaggtg ttgatttttc gcgtctgatt ccagggattt tgattatctc tatgaatagt
     3721 ggtgcttatg tttcggagat tgttcgtgcc ggaatcaatg cggttccaaa aggtcagcta
     3781 gaggcggctt attcgctagg gattcgtcct aaaaatgcga tgcgttatgt gattttgcca
     3841 caagcagtca aaaatatctt gccagcattg gggaacgaat ttatcaccat tatcaaggac
     3901 agctccctct tatcagctat tggggtcatg gagttgtgga atggggctac aacagtttct
     3961 acaacaacct atctaccttt aacaccactt ttatttgcag cattttacta cttgattatg
     4021 acctctattc tgacagtagc cttgaaagct tttgaaaaac atatgggaca aggagataag
     4081 aaataatgac agaaaccttg ataaaaattg aaaatttaca taaatctttt ggaaagaatg
     4141 aagtattgaa gggcatcaac ctcgagatta aaagaggaga agttgtcgtt atcatcggtc
     4201 cttcagggag tgggaaatct accttgcttc gctctatgaa tttgttggag gaagcaacca
     4261 aggggaaggt tatctttgag ggagtcgata ttacggacaa gaagaatgac ctgtttgcca
     4321 tgcgtgagaa gatgggcatg gttttccaac aatttaacct ctttcctaat atgactgtga
     4381 tggaaaatat taccttgtca cctatcaaga ctaaaggtga aagcagggaa gttgctgaga
     4441 agagagccca agagcttttg gaaaaagttg gtttgccaga taaggcagac gcttatccac
     4501 agagtctgtc gggtgggcaa caacaacgga ttgccatcgc gcgtgggttg gctatggaac
     4561 cagatgtttt gctctttgac gaaccaactt cagccttgga tcctgaaatg gtaggtgagg
     4621 tattggctgt tatgcaagac attgccaagt cagggatgac tatggttatc gtaacacatg
     4681 agatgggatt tgcccgtgag gtggcagatc gtgttatctt tatggcagac ggtgtggttg
     4741 ttgaagatgg gacacctgag cagatttttg aacaaaccca agaacaacgg actaaagatt
     4801 tcttgagtaa ggttttataa tctattttaa aatttcaaga caagattagt gaaaagttca
     4861 accagagctt tttcttatag tttgaaacta taggataacc taggaaagaa gtgttagagg
     4921 gcactagcag tttttggtat aatggaagat atttgaaaga aaagagaagt gatatgacac
     4981 agattattga tgggaaagct ttagcggcca aattgcaggg gcagttggct gaaaagactg
     5041 caaaattaaa ggaggaaaca gctctagtgc ctggtttggt agtgattttg gttggggata
     5101 atccagccag ccaagtctac gttcgcaaca aggagaggtc agctcttgcg gctggttacc
     5161 gtagcgaagt agtgcgagtt ccagagacca ttactcaaga ggaattgtta gacctgattg
     5221 ctaaatataa tcaggatcca gcttggcatg ggattttggt ccagttgcca ttaccaaaac
     5281 atatcgatga agaggcggtt ttattagcca ttgacccaga aaaggatgtg gatggtttcc
     5341 atcccctaaa catgggacgt ctttggtctg gacatccagt catgattccc tcgacacctg
     5401 caggaattat ggaaatgttt catgaatatg ggattgactt ggaaggtaaa aatgcggtcg
     5461 taatcggtcg ttcaaatatc gttggaaaac ctatggctca gcttcttttg gctaaaaatg
     5521 cgacagtaac cttgacccac tcacgaactc ataatcttgc caaggtggct gctaaagcag
     5581 atattcttgt agtcgcaatc ggccgcgcca agtttgtgac tgctgacttt gtcaaaccag
     5641 gtgcggttgt cattgacgtt gggatgaatc gagatgaaaa tgggaagctc tgtggagatg
     5701 ttgattatga ggcagttgca ccacttgcta gccatatcac gccagtccct ggaggtgtcg
     5761 gtcctatgac cattaccatg ctgatggagc aaacttatca agcagcactt cggacattgg
     5821 atagaaaata agagaagcat tcgattaata gagtgtattt tctatagcta tatctaaaat
     5881 ggcagaaatg aatattaaat tttagatata agattacaaa aggaggtctg cgtctccttt
     5941 ttattgtata ataaaagtga gaggaaaaaa ggatgaaagt gattaatcaa accttattag
     6001 aaaaagttat tattgaacgt tctcgttcaa gtcataaagg agattatggt cgtctgctgc
     6061 tgcttggtgg tacttatcct tatggaggtg ccatcatcat ggctgcttta gcagctgtaa
     6121 aaagcggtgc aggattgatg accgttggaa cggacaggga aaatatcccg gctctgcaca
     6181 gccatttacc tgagcctatg gccttttctc ttcaagacca gcaattgtta aaagagcaat
     6241 tggagaaggc agaagttatc ttgctggggc ctggtttagg agacaatgct tttggagaag
     6301 atttagttaa acaggtcttt gctggcttaa aacagaatca gattttgatt gtagacggag
     6361 gtgccttgac cattcttgct aggacaagtt tgtcatttcc ctcaagccag cttatcctaa
     6421 ctcctcacca aaaagaatgg gaaaaattat ctggaattac gattgaaaag caaaaggaag
     6481 atgcaacggc tagtgttctg acttccttcc ctcaaggaac aattttggtg gagaaaggcc
     6541 cagctactcg tatttgggaa gttggccaat ctgattatta ccagttacag gttggtggtc
     6601 cttatcaggc gactggggga atgggagata ctctggctgg gatgatcgca ggatttgtag
     6661 gccaattccg acaggccagt ctctacgagc gtgtggcagt agcgacccat cttcattcag
     6721 ccatagccca agaactatct caagaaaatt atgtggtctt accgacggaa atcagcaggt
     6781 atcttcccaa aataatgaaa attatttgtc aacaagagag ggtaagcaaa gataaacttg
     6841 tttaaaatag acagataaat ctacttcctt ctcttaattt atgattcttg aaatgcgcct
     6901 gctttaaggc taaactagac gtatcaaagt taagagtttg tttttgtaac tggaaagaga
     6961 tgaacaaatc aaatacataa aagataaact catttttatt cgttttgtaa aaataaaatg
     7021 catattttta aagaaagatt gatgaaaaat gtagatttga tgtttttact tgaataaaaa
     7081 gcggtttcat agtaaaataa gaactgagaa tgatggaggt aaatcggtgg aaaatctgaa
     7141 gaaaatggca ggtatcaagg ctgctgagtt cgtgagcgat ggaatggtcg ttggacttgg
     7201 aacaggctcg actgcctatt attttgtcga agaaatcggt cgtcgtatca aggaagaagg
     7261 attgcagatt acagctgtaa caacttctag tgtgaccact aaacaggcag aaggccttaa
     7321 tatcccgctc aagtctattg accaagtaga ctttgtcgat gtgacggttg atggcgcgga
     7381 tgaagtggat agtcagttta atggaatcaa aggcggtggt ggtgcccttc tcatggaaaa
     7441 ggtggtcgca acaccatcaa aagaatacat ttgggtggtg gatgaaagca agctggtcga
     7501 aaaactaggt gcttttaaat tgccagtaga agtggttcag tatggtgcag agcaggtctt
     7561 tcgtcatttt gaacgagctg gctacaaacc aagtttccgt gaaaaagacg gtcaacgttt
     7621 tgtgacggat atgcagaatt ttatcattga cctcgccttg gatgtcattg aaaatccaat
     7681 tgcttttgga caagaattgg accatgtcgt tggtgttgtg gagcatggtt tattcaacca
     7741 aatggtggat aaggtaatcg ttgctggacg agatggagtt cagatttcaa cttcaaaaaa
     7801 aggaaaatag aagggggcat aagatgtcta aatttaatcg tattcatttg gtggtactgg
     7861 attctgtagg aatcggtgca gcaccagatg ctaataactt tgtcaatgca ggggttccag
     7921 atggagcttc tgacacactg ggacacattt caaaaacagt tggtttgaat gtcccaaaca
     7981 tggctaaaat aggtcttgga aatattcctc gtgaaactcc tcttaagact gtagcagctg
     8041 aaagcaatcc aactggatat gcaacaaaat tagaggaagt atctcttggt aaggatacta
     8101 tgactggaca ctgggaaatc atgggactca acattactga gcctttcgat actttctgga
     8161 acggattccc agaagaaatc ctgacaaaaa tcgaagaatt ctcaggacgc aaggttattc
     8221 gtgaagccaa caaaccttat tcaggaacgg ctgttatcga tgattttgga ccacgtcaga
     8281 tggaaactgg agagttgatt atctatactt cagctgaccc tgttttgcag attgctgccc
     8341 acgaagacat tattcctttg gatgaattgt accgtatctg tgaatacgct cgttcgatta
     8401 cccttgagcg tcctgccctt cttggtcgca tcattgctcg cccttatgta ggtgaaccag
     8461 gtaacttcac tcgtacggca aaccgtcgtg acttggctgt atctccattt tccccaactg
     8521 ttttggataa attgaatgag gctggtatcg atacttatgc tgtgggtaaa atcaacgata
     8581 tctttaacgg tgctggtatc aaccatgaca tgggtcacaa caagtcaaat agtcatggaa
     8641 ttgatacact attgaagact atgggacttg ctgagtttga aaaaggattc tcattcacaa
     8701 acctagttga ctttgatgcc ctttacggcc atcgtcgtaa tgctcacggt taccgtgatt
     8761 gcttgcatga gtttgatgaa cgcttacctg aaattatcgc agctatgaga gagaatgacc
     8821 ttctcttgat tactgcggac catggaaatg acccaacgta tgcaggaacg gatcacactc
     8881 gggaatatat tccattgttg gcctatagcc ctgcctttaa aggaaatggt ctcattccag
     8941 taggacattt tgcagatatt tcagcgactg ttgccgataa ctttggtgtg gaaactgcta
     9001 tgattgggga aagtttctta gataaattgg tataagatga cgcgctatgc tttgctggtg
     9061 agaggtatca atgttggtgg taagaataag gtcgtcatgg cggagcttcg tcaagaattg
     9121 acaaacttgg gactggaaaa ggttgagagc tacatcaata gtggcaatat tttctttact
     9181 tcgatagatt ccaaagccca attggttgaa aagctagaga ctttctttgc agtccattat
     9241 ccatttattc agagcttttc tttactgagt ctagaggact ttgaggcgga acttgaaaat
     9301 ctaccagctt ggtggagcag agacttggca cgaaaagatt ttctctttta cactgagggt
     9361 ttggatgtgg accaagtcat cgcgacagtt gaaagtttag agctgaaaga tgaagtgctt
     9421 tattttggaa aacttgggat tttctggggg aaattttctg aagaatccta ttctaagact
     9481 gcctatcata agtacttgct gaaggtgcct ttctaccgcc acattactat tcgtaatgct
     9541 aaaacctttg acaaaattgg tcaaatgcta aaaaaataat aaaggagaca cacaatgaca
     9601 tttttaaaca aaatccatga aactgctact ttcctgaaag aaaagggaat tgcagcccct
     9661 gagttcggtc taatccttgg atcaggactt ggagaattgg cagaagaaat cgaaaatcca
     9721 gttgtagtag actatgctga gattccaaac tggggccgtt caacagtagt cggtcatgct
     9781 ggtaaattgg tatatggtga actggcaggt cgcaaggtct tggctcttca agggcgtttc
     9841 catttctatg aagggaatcc tctggaagtg gtgactttcc cagttcgtgt gatgaaagtt
     9901 cttggatgtg aaggtgttat tgtaaccaat gcagctggcg gtatcggatt tggtcctggt
     9961 accttgatgg ctatctcaga ccatatcaac atgacggggc aaaatccatt gatgggtgaa
    10021 aacttggatg actttggccc acgtttccca gatatgtcta gggcctacac accagaatac
    10081 cgtgccactg cccatgaagt ggctaaaaaa cttaatatca agcttgatga aggtgtctat
    10141 atcggagtta ctggtccgac ttatgaaaca ccagcagaaa ttcgttccta taagacactg
    10201 ggagcagatg cagttggtat gtctacggtt cctgaagtta tcgtggcagc ccactctggc
    10261 ttgaaagttc tgggaatttc atgtatcact aactttgcgg ccggtttcca agaagaactc
    10321 aatcacgaag aagttgtaga agtgactgaa cgtgttaaag gtgatttcaa aggcttgctt
    10381 aaagcgattc ttgctgaatt gtaagaaaaa agatttaaaa gggggagtgc ctctgttttt
    10441 tcaggattga ctgcctatcc ggattaaaga agaaacagag gaatactatg agcttcttcc
    10501 tgctcttata actgaaagaa gcggaagaat aggtatgtct gatctgatag ccagcattgt
    10561 gaaagacaag attctaggat actagcatta gcttcctagc caagcagact agtatgataa
    10621 ggagagatga gaatgaattg actttctgaa tttctcagtc ttatcatata tagcacaatg
    10681 agatttcgct tgagtctgct tgtaaataaa cgaaaagaaa gataagaaat aatgaaaatt
    10741 ggtcaacgaa ttatgcgctt tggcataaaa aattaagtat cggagttgta tctgttgtag
    10801 tcggctttga tttctagctc cagctggaat ttcagccaat gaagtaaagc aagatgtaac
    10861 atctgaagtg gtaataggtg tgctagattc taaggaggaa ttgaaagagt cagaaaatga
    10921 tgctccaaaa ctagaaactc ctcttagaga ggagccaaga ctagctcctc aaacgcttcc
    10981 ggaagcaagt gaagttcttg aaaacaaaag ggaagagtca aaagtagaga taacataacc
    11041 agctcaagcg gatgatatcc gcaaggttgt tggggaatta gccaaggata taagtattac
    11101 taagttgtat atgacaggtc attctcttgg atgttaccta gctcagattg cagcggttga
    11161 agcttaccaa aaatatcctg atttttataa ccatgtattg aggaaagtga caactttcag
    11221 tgctcctaaa gtgattactt ccagaactgt ttggaatgct aagaatggtt tctgggatgt
    11281 tggtttggaa agtcgtaaat tagctgttag tggaaaaatt aagcattatg tggttgataa
    11341 tgacaatgtt gtgactccct tgattcataa taatcgtgat attgttacat ttacaggtaa
    11401 ttcacgcttt aaacaccgtt ctcgtggcta ttttgaaagt ccaatgaatg atattcctaa
    11461 ctttaatatt ggtaaacaag ctaccttgga taaacatggt tatcgtgatc cgaaattgga
    11521 taaagtgcga ttctttaaga aacaggctct gcctcgatct tctagtcaac caagcgctga
    11581 accaatggaa aatattgcct taggaaaaca ggttactcaa agttcgacag ctttcggagg
    11641 agatgctaga agagctgtgg atggcaaagt cgatggtaac tatggtcaca attctgtcac
    11701 tcatacaaac ttccaatcta agccttggtg gcaagtagat ttggctaaag aagaaaccat
    11761 tcgccaaatc aatatttaca accgaacaga cactgcccag gatagattgg caaactttga
    11821 tgtcattctt ttagacagtt ctggtaaaga aattgagtga aaacgtataa tatctcctaa
    11881 agatgtgtca gcacaaatta cgattaacca taaaaaagcg cgctatgttc ggattgagct
    11941 agaaggctat aatgccctca gtcttgcaga agttgaagtt ttccgcttta tagctacgaa
    12001 tgctgaaacg gcgacacaag tttctaagcc agttcaacca atcagtcaga ctcctgtgaa
    12061 agataaaaca ttgacaattc aacacagtgg agcttacatt gcccgctact ccataacttg
    12121 ggaagaagtt ccagtagata aagatggaaa ccaagttgtt cgtagtcatt cttgggaagg
    12181 aaacggtcgc aaccagactg cgggttttgt cctcaacctc ccaatcaaag aaaatatgag
    12241 aaatctgcga gttaagattg agaaaaagac gggcctacta tggaatagat ggcaaacaat
    12301 ctatgaaaac agaccaattt cagctcaacc ccaccgtaaa attacccatt ggggtacgac
    12361 attgaattcc aaggtgagtg acgatgatgt cttgtaatct gatggtacaa tgacagttag
    12421 tttgtctagt ttataagaaa gtactacctg agcttgaata gaactcaggt agctctctgt
    12481 gaaagaacaa aattaatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
    12541 ttgctcaaag cactgctttg aggttgtaga taagactgac gaagtcagtc acatatataa
    12601 tccaaggcga cgttgacgtg gtttgaagag attttcgaag agtataaaca gaaaggtaga
    12661 gcgcgtgttc taatttgaac acgagtagaa aacttttcta aaaacaaaaa cgaaaggatg
    12721 ggtaaactgt attcgctgaa ctgaatacgg gcgactctcc tctaaatcaa aattaagaaa
    12781 ggaattgacc ccaccctaaa agtagtggga aaaagatagt tggtctagcg agcatcgctc
    12841 actgcgccca actcctattt tcccttcgct ttttgatggg tttggtatct ttctcaatat
    12901 aaaataaaga aaggtagagc gtgtgttttg atttgaacac gagcggaaaa ctcggaaaat
    12961 agataatctg actgaaaaat caggatttct cgtcaggttc ctaattttca gtcgttttct
    13021 tctcgctctt tgtatcataa attatgtcta tccatattgc tgctcagcag ggtgaaattg
    13081 ctgataaaat tcttcttcct ggggatcctc ttcgtgctaa gtttattgcg gagaatttcc
    13141 ttgatgatgc tgtttgtttt aacgaagtgc gtaacatgtt tggttacact ggtacttaca
    13201 agggtcactg tgtatctgtc atgggaactg ggatgggaat gccatctatt tcgatttatg
    13261 cgcgtgagtt aatcgtagac tacggtgtga agaaattgat tcgtgtggga actgcaggtt
    13321 ctttgaatga agaggttcat gttcgtgaat tagttttggc gcaggcggct gcaaccaact
    13381 caaacatcgt tcgtaatgac tggccacagt acgattttcc acaaattgct agctttgatt
    13441 tgcttgataa agcctaccat atcgccaaaa aacttggtat gactactcac gttgggaacg
    13501 ttttgtcatc tgatgtcttt tactcaaatt actttgaaaa gaatatcgag cttggtaaat
    13561 ggggagtcaa ggctgtggaa atggaagcag cagctcttta ctatcttgct gcccaatacc
    13621 atgttgatgc gctagctatc atgaccatct ctgatagctt ggtcaatcca gacgaagaca
    13681 caactgcaga agaacgtcaa aataccttca ctgatatgat gaaggttggt ttggaaacct
    13741 tgattgcaga ataattatag ccaaaaagga agccctttat gaattgtagg acttcctttt
    13801 cttatccaga aattgatcta gctctctctg atttcgaaga atagtgactt tatgtgaata
    13861 ttcttggcaa agtttttggt aattttcttt ttgagttttg ctacgcccat cccaaagaat
    13921 ccatctgata aactcccact caaagcgttc agggcaatct gccgccatac tttctctgac
    13981 ttttccacgg tatttaagat aacgcttaaa ggctctaaag agacaggtca atggcgaaaa
    14041 attgagaaag atgatttggt cagcttcttg cattcgttct tggtagtagc accaagaata
    14101 attaccatcg atgacccaag ctttatgctt ggtgagaaag ttttttatct cggttaacat
    14161 ccattcgcag tcactgtctt gccaaccagg ttgaaattgg agtgtgtcca tgtgcagttt
    14221 tggaatggag tagtagttag ataacttttc tgctatagtt gacttaccag aaccagaata
    14281 tccgataatt gcgattttca ttttctactt tttcctattt ggagacaaaa aaacagcctc
    14341 tatggactgt ttcttattta gcaagtttag ctgaaagacg agctttatcg cggcttgctt
    14401 tgtttttgtg aatcaaacct ttagtttctg ctttatcgat agctgagcta gcagcacgga
    14461 aaagttcttc agatgggttt gcttcgaaag cttttatagc agtacgcata gctgattttt
    14521 gagctgagtt cttttcgttt tgtttaacgt tcaattcagc gcgtttgata gctgatttaa
    14581 tgtttgccaa tgttcttacc tccatattta ctaactatac cattatatat gaaaacttac
    14641 gttttgacaa gggaaaatta cttttttaag taaatttcat cgatttcatg gttcttgctt
    14701 ttatgaagaa tcacttctgc acgatttctg gttggttcaa tataattttg cagatttgtg
    14761 agattgatac tgatccagac ctgatgggca aaggactcca cttccccaat cggcatctga
    14821 gtaaaacgat aatagtagct atcagggtcg ttttgggcta ggctcagcat tttcaagaaa
    14881 cggtccagat accaactttc aatatcatcc actccagcat ctacatagat ggaaaagtca
    14941 aagaagtcag tgatatagag acgatcgttt tgtggatttt gaaagacatt aattccctca
    15001 acaattacaa aatcagcagc tttgacactt tgtttctttt cgggtacgat gtcgtaaact
    15061 tcatgagaat agacaggaat atctacatct tgtccatttt tgatgcggtc caagaagttg
    15121 agaagagctt ccatatcata gctttcagga aatcctttac gatttaaaat cccctgctca
    15181 atcaaggttt gattgggata gagaaaacca tcagttgtaa ccaactcaac cgtagcatct
    15241 gtaaacgtac gggacagtag gatttgaagt aggcgactgg ttgtggattt tccaacggca
    15301 acactcccag aaaccccaat aataaaaggt tgagatttac tttcacgttg gaggaaaatt
    15361 ccttttgaaa aggctaaatc ttccttagtt cgcttgtaaa tctgaatcag atgagccaag
    15421 gggagataga tatctgtaac gtcttggaga ctgatttggt cattaaaact cttgatagat
    15481 tccaattctt cttctgtcaa aggaggtgtt gtctttcgat gtaaagattg ccaagtctgg
    15541 cggctgattt tttcaaaatg taaaaattcg ttggtcattt gttttcccct agatattttg
    15601 tctatcatac cataaaaagc taaaaaaata aagcggtttc ttgaatgtgg taagtcaatg
    15661 gtatatagta taagtgatca atttattgat gaaataggag atagattcta ctgatttata
    15721 agcttataaa gggggctttt tagttcacaa cttataggag gtgtactatg aaaatcttaa
    15781 aacgttacat attggaactc tgttttattt taagttttgc tttacctttt ataaaaggaa
    15841 ccaatgcaga taatggtaga tgctttgtgg aaacctatta cggttttact tttttgatgg
    15901 aacatgctat tgtaacagct gtctttatct attcgttttt aattgctttc ttactaaaaa
    15961 aacgatgggc gaaatggatt gctgctggta gttattgctt tttagtccta tggattgcga
    16021 cagagggtta tttcttccgc atgtcactag aagatttgat acgactttgg acaagtttgg
    16081 aaatcctgac acaaacgtat cagttgggct tttatctaaa cattttgtta ggaattttgt
    16141 tgataataaa gtattttaag gttaagcaat agaaataata atctgcctga ttatagatga
    16201 taaacttttt gtttttatct ggactgtgat atttatagtt ctaaatggtt tattatttat
    16261 aagtgttgaa agagattgaa gaaaatggct tcgtgacttg ctttcttcgg tctttttcgt
    16321 tttattatca atttgtaaac gatttacaat ttgagtcaaa ttatggtaaa atagtgtcat
    16381 gagtaaaatg tattatgcag aaaatcctga cgctgctcac gacattcatg agttgagagt
    16441 ggacttgttg ggacaaaaaa tgactttttt gacagatgcg ggtgttttta gcaagaaaat
    16501 ggttgacttt ggaagtcaac tcttgctcaa gtgtctggat gtcaaccaag gagagacagt
    16561 cctagatgta ggctgtggtt atggtccatt aggtttgtcc ttggtcaagg tttacggagt
    16621 tcaggcgacc atggttgata ttaacactcg tgccttggat ttagctcgga gaaatgctga
    16681 aaaaaataat gcaaaagcga cgattttcca gtccaacatc tatgaacaag ttgaaggaca
    16741 ttttgatcat gtcatttcca atcctcctat ccgtgcgggc aagcaagtcg ttcatgaaat
    16801 cattgagaaa agtaaagatt tcttggaaac tggtggggat ttgacaatcg tcatccagaa
    16861 aaaacaaggg gctccaagtg ctaagtccaa gatggaggat gtgtttggta attgtgaaat
    16921 ccttaaaaaa gacaaaggat actatatcct tagaagtgtg aaagaatgag agcagttgat
    16981 ttaatacaaa aaaaacgaga cggtcaagaa ctgacttcaa gtgaaattga atggctggta
    17041 gaaggctatg tgtcaggaac tgttcctgat tatcagatgt ctgcctttgc tatggctgtt
    17101 tattttaaag gaatgacgac tcgtgaaatt tctgatttaa cgatgaatat ggttaagact
    17161 gggcaagagt ttgacctgtc agccattgat ggtgtgaaag ttgacaagca ttctactggt
    17221 ggtgtcggcg ataaggtaac cttgatttta gctcctcttg ttgctagctt cggtgtgcct
    17281 gtagcaaaaa tgagtggtcg tggtcttggc catacaggcg gaacaattga taaattggag
    17341 tccattaggg gctatcaagt agaacgtagt caagaggatt ttattcgtca ggttcaggat
    17401 attggagtat ctgtcattgg gcaatcagac cagctggtca aagcagataa gcttctctat
    17461 gcccttcgtg atgtgaccgc aactgtcgac acgattcctt tgattgcgag ttcggtgatg
    17521 agcaagaaaa ttgcggcagg ggcggatgct attttgctag acgtgactgt cggtgagggt
    17581 gccttcatga agacggttga tgaggcgcgt gagttggctc aaactatggt agatcttggt
    17641 aaggttgttg gacggaagac agtagcagtg attacggata tgagccagcc tttgggacga
    17701 gcgattggaa atcgtctgga aatacttgaa gcattggaga ttttgcaagg tcaaggacgt
    17761 caggatatta cccactttat ttgtgaattg gctcaaatta tgcttggttt ggcaaatgtt
    17821 aacaagactg ttgaagaagt tcaccaacat cttgagaatg gtcaagcact ggctaagttt
    17881 gaggaaatgg tgcaagccca aggtggagac ttggaagacc tctatcgtcc tgtaaatgta
    17941 gctcatgtgg tggaaatccc tgctcaggaa acgggtgtca tttcagctct tccagctatg
    18001 gactttggtc tttatgccat gaggctgggg gctggtcgtg cagttaagtc tgatgccttg
    18061 gactatgaaa caggaattgt ttttgaaaag aagattggag actccgttca aaagggtgaa
    18121 attgttgcaa aagtatacac aaatgaaaaa attccacctc aactagttac agatttccaa
    18181 aaatgtgtta aaataagtga tgaagtgaaa aaaatccgtg aaattgtaga aatcatctcc
    18241 taaatggagg gaaaaccgaa atgaaattaa ataaatatat agatcatacg cttttaaaac
    18301 aagatgcaaa gaaaaaacaa attgatagtt tgttgtctga ggctagagag tatgactttg
    18361 ccagtgtttg cgttaatccg acctgggttg aacatgctaa aaaaggactt gaaggcacag
    18421 atgttaaggt ttgcacggta gtaggtttcc ctttgggagc aacaacttca gccgtgaaag
    18481 catttgagac aaaagaagct atccaaaatg gtgcagatga gattgatatg gtgatcaatg
    18541 ttggagctct caaatcaggt aatttagcct tggttgagtc agatattcgc gcagtagtgg
    18601 aagcaagtgg tgataagtta gtgaaagtca ttattgaagc ttgccttctg acagaccaag
    18661 aaaaaattgt tgtttgccaa ttggcccaaa aagctggggc tgactttgtt aaaacatcta
    18721 ctggtttttc aactggtggt gctacgatag cagatgttac attaatgcgt gaaacagttg
    18781 gatctgatat gggagtcaag gctgctggtg gagctcgttc ttatgcagat gctcttgcct
    18841 ttgtcgaagc aggtgcgacc cgtatcggaa cgtcagctgg ggtagctatt ttaaaaggag
    18901 aattggcaga tggcgactac tgagttgatt gaactggcaa ttgaaaccag caaacatgcc
    18961 tatgtcccct attctcactt tcctatcgga gcagttttag tagccaaaga cggaagtgtt
    19021 tacacgggag tgaatatcga aaatgctagc tatcctttga ccaattgcgg tgagagaaca
    19081 gctattttta aggctatatc tgaaggccaa agagaatttt cagaattgat tgtctatggt
    19141 caaactgaaa aaccgatctc gccatgtggt gcttgtcgcc aagtgatggc tgaatttttt
    19201 gaacaagatc taaaagtgac cttagtcgca aaagataaat cgacggtcga gatgacggtc
    19261 ggggaattac ttccatactc ttttacagac ttaaactagt ctgagtcgct ctctgagtgg
    19321 cacggtcctt gtggccaacc aatccatact tgcaacatcg ttgcacatct tatttaggag
    19381 gttcagtaat gaacaagaaa caatggctag gtcttggcct agttgcagtg gcagcagttg
    19441 gacttgctgc atgtggtaac cgctcttctc gtaacgcagc ttcatcttct gatgtgaaga
    19501 caaaagcagc aatcgtcact gatactggtg gtgttgatga caaatcattc aaccaatcag
    19561 cttgggaagg tttgcaggct tggggtaaag aacacaatct ttcaaaagat aacggtttca
    19621 cttacttcca atcaacaagt gaagctgact acgctaacaa cttgcaacaa gcggctggaa
    19681 gttacaacct aatcttcggt gttggttttg cccttaataa tgcagttaaa gatgcagcaa
    19741 aagaacacac tgacttgaac tatgtcttga ttgatgatgt gattaaagac caaaagaatg
    19801 ttgcgagcgt aactttcgct gataatgagt caggttacct tgcaggtgtg gctgcagcaa
    19861 aaacaactaa gacaaaacaa gttggttttg taggtggtat cgaatctgaa gttatctctc
    19921 gttttgaagc aggattcaag gctggtgttg cgtcagtaga cccatctatc aaagtccaag
    19981 ttgactacgc tggttcattt ggtgatgcgg ctaaaggtaa aacaattgca gccgcacaat
    20041 acgcagccgg tgcagatatt gtttaccaag tagctggtgg tacaggtgca ggtgtctttg
    20101 cagaggcaaa atctctcaac gaaagccgtc ctgaaaatga aaaagtttgg gttatcggtg
    20161 ttgatcgtga ccaagaagca gaaggtaaat acacttctaa agatggcaaa gaatcaaact
    20221 ttgttcttgt atctactttg aaacaagttg gtacaactgt aaaagatatt tctaacaagg
    20281 cagaaaaagg agaattccct ggcggtcaag tgatcgttta ctcattgaag gataaagggg
    20341 ttgacttggc agtaacaaac ctttcagaag aaggtaaaaa agctgtcgaa gatgcaaaag
    20401 ctaaaatcct tgatggaagc gtaaaagttc ctgaaaaata atggatggaa gtcatttctt
    20461 aggaagcggc caagggccgc ttttttaaga gttgagacaa agtcattttg tctcagtaaa
    20521 gcttgctact agacaaacta gcaagtttta ttgaaataaa ataagactct gaaaggaaga
    20581 gcacatggca cacgaaaatg tcattgagat gcgtgatatt accaaggtgt ttggtggatt
    20641 tgttgccaac gacaaaatca acttgcacct acgaaaaggt gaaattcatg cacttttagg
    20701 agaaaatggg gctggtaagt ccacgctaat gaacatgtta gcaggccttc ttgaaccaac
    20761 tagtggtgaa atcgcggtca acggtcaagt tgtcaatctc gactccccat ctaaagcagc
    20821 tagcttggga atcgggatgg ttcaccagca ctttatgttg gttgaagcct tcacagtggc
    20881 tgaaaacatc attttaggta gtgaattgac taaaaatggt gtgctagata tcgctggagc
    20941 tagcaaagaa atcaaggctc tttctgaacg ttatggctta gctgttgacc cttctgccaa
    21001 ggtagcagat atctcagttg gagcccaaca acgtgtagaa attttaaaaa cactttatcg
    21061 gggggctgat atccttatct ttgacgaacc aacggctgtt ttgactccat cagaaattga
    21121 tgagttgatg gctattatga aaaatcttgt caaagaagga aaatcaatta tcttgattac
    21181 ccacaaattg gatgaaattc gcgcagtttc tgaccgtgtt acagttatcc gtcgtgggaa
    21241 atcaattgaa accgttgaaa ttgcaggggc taccaatgct gatttggcgg aaatgatggt
    21301 aggacgttct gtttccttta aaacagagaa gcaagcctct aaaccaaaag aagtggtttt
    21361 gtctatcaaa gatttggtgg tcaatgaaaa ccgtggtgtt ccagctgtta aaaatctgtc
    21421 cttggatgtt cgtgctggag agattgttgg tattgcgggg attgatggaa atggtcagtc
    21481 tgaactgatt caagccatta caggtcttcg taaggttgaa tctggtagca ttgagctaaa
    21541 aggagattca attgtaggct tgcacccacg tcagattaca gaactaagtg ttgggcacgt
    21601 tccagaagac cgtcaccgtg atggcttgat tttggaaatg atgatatctg aaaatattgc
    21661 ccttcaaacc tactataaag aaccacatag taaaaatgga attttgaatt attcaaatat
    21721 tacttcttat gctaaaaagc tgatggaaga gtttgatgtt cgcgctgcca gtgaattagt
    21781 tcctgcagct gcactctcag gaggaaatca acaaaaagca attattgctc gtgaaattga
    21841 tcgagatcct gatctcctta tcgttagcca gccaactcgt gggttggatg tcggtgccat
    21901 tgagtatatc cacaaacgct tgattgaaga gcgtgataat ggcaaggctg tccttgttgt
    21961 cagctttgaa ttggatgaga ttttaaacgt ctcagaccgt attgccgtta tccacgatgg
    22021 taagattcaa ggtattgtat caccagaaac aaccaataaa caagaacttg gtgtcttgat
    22081 ggctggtgga aacttgggaa aggagaagag tgatgtctaa aaaattacaa caaatttcgg
    22141 ttcccttgat ttctgtattt ctaggaattt tactcggagc cattgtcatg tggatcttcg
    22201 gttatgatgc tatttggggc tacgaagaat tgttctatac agcctttggc agtctgcgtg
    22261 ggattggaga aatcttccgt gctatgggtc ctctggtctt gattggtctt ggttttgcgg
    22321 ttgccagtcg agctggtttc tttaacgtcg gacttcctgg tcaggctttg gcaggttgga
    22381 ttctcagtgg ttggtttgcg ctgtcgcatc cagatatgcc ccgtcccttg atgattctag
    22441 caaccatcgt gattgccttg attgctggtg ggattgtcgg agcgattcca ggtattctta
    22501 gggcctatct agggacgtca gaggttattg taaccatcat gatgaactac attgtcttgt
    22561 atgtagggaa tgcctttatc catgctttcc ctaaagactt catgcaaagt acagattcga
    22621 ccattcgtgt tggggctaat gcaacctatc agacaccttg gttggctgag ttgactggta
    22681 actcacggat gaatattggt attttctttg ccatcattgc cgttgcagtt atttggttca
    22741 tgctcaagaa aacaactctt ggttttgaaa tccgtgcagt tggtcttaat ccacatgctt
    22801 cagaatatgc tggtatttct gccaagcgga ctattatcct atctatgatt atttcaggtg
    22861 ccttggcagg tcttggtgga gctgttgaag gtttgggaac cttccagaac gtctatgttc
    22921 aaggttcgtc attagctatc ggatttaacg gaatggcggt tagtttgctt gcggccaact
    22981 caccaattgg tatactcttt gcagccttcc tatttggcgt tctccaagtt ggggctcctg
    23041 gtatgaatgc ggcgcaggta ccatctgagc ttgtcagcat tgtaacagcg tctattatct
    23101 tctttgtcag tgttcattac cttatcgaac gctttgtcaa accgaaaaaa caagttaaag
    23161 gaggtaagta aagatgtcta ttataacctt gctccccctc ttggtgtctt ctatgctgat
    23221 ttactcagca cctctcatct ttacaagtat cggtggtgtt ttctctgaac gtggtggtgt
    23281 ggtaaacgtt ggccttgaag gaattatggt tatgggtgcc ttttctggag ttgtctttaa
    23341 ccttgaattt gcagaacaat ttggagcagc aactccatgg ctatccttgc ttgtagcagg
    23401 attggttggt agtgttttct ctatcatcca cgcagcagcg acggttcatt tccgtgcaga
    23461 ccatgttgtc agcggtacgg tattgaactt gatggcgcct gccttggctg ttttcttagt
    23521 taaagttctt tataacaaag gacaaaccga caacctaagt caaacttttg gacgctttga
    23581 tttcccagtc ttggcaaata tcccagtgat tggtgatatc ttcttcaagt caactagtct
    23641 acttggttat ctggcgattg ccttctcatt ccttgcttgg tttattctct ttaagactca
    23701 atttggtctt cgtctccgct ctgtcggtga acatcctcaa gcagcggata ccttgggaat
    23761 caacgtctac aagatgagat atttagggat tattatttca ggttttctag gtggaattgg
    23821 cggagcgatt tatgcgcaat caatctcagt taacttctca gtgacaacta ttgttggacc
    23881 tggatttatc gcccttgctg cgatgatctt tgggaaatgg aatccaatcg gtgctatgct
    23941 ttctagtctc ttctttggac tttcacaaag tttggctgtt atcggttctc aattgccgtt
    24001 cctacaagga gtgccagcgg tttatcttca aattgcacct tatgttttga caattcttgt
    24061 cttagcagcc ttctttggaa aagcagtcgc accaaaagca gatggtatca actacatcaa
    24121 atcaaaataa gcatacaaaa aaacgtcagt ccaatgactg aactgccccc caaaagttag
    24181 acagaaaaaa tctaactttt ggggtg
//
LOCUS       DAVBCB010000031        23727 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVBCB010000031 DAVBCB010000000
VERSION     DAVBCB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23727
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.031"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..222)
                     /locus_tag="VQU28_001211"
     CDS             complement(<1..222)
                     /locus_tag="VQU28_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476571.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDFQKPSKIPYLETTGMPTRIL"
     gene            538..798
                     /locus_tag="VQU28_001212"
     CDS             538..798
                     /locus_tag="VQU28_001212"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2476572.1"
                     /translation="MSGGETKRVALAKIILKNPPLIFADELTASLDSTTSQGIMKLLL
                     GMKNKNRLIIIATHNPDIWNQADEVINLNQLLQSIYIFYILK"
     gene            complement(902..1372)
                     /locus_tag="VQU28_001213"
     CDS             complement(902..1372)
                     /locus_tag="VQU28_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2476573.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQTLLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(1389..3662)
                     /locus_tag="VQU28_001214"
     CDS             complement(1389..3662)
                     /locus_tag="VQU28_001214"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HET2476574.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLASDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQ
                     AIQNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            4009..7110
                     /locus_tag="VQU28_001215"
     CDS             4009..7110
                     /locus_tag="VQU28_001215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HET2476575.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQGVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRLAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQVMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEVREGAFYYIKGKVQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHESDQEISRILEQFKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNEIVMKT
                     IYR"
     gene            7192..8199
                     /gene="pfkA"
                     /locus_tag="VQU28_001216"
     CDS             7192..8199
                     /gene="pfkA"
                     /locus_tag="VQU28_001216"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HET2476576.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            8256..9761
                     /gene="pyk"
                     /locus_tag="VQU28_001217"
     CDS             8256..9761
                     /gene="pyk"
                     /locus_tag="VQU28_001217"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HET2476577.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(10493..10718)
                     /locus_tag="VQU28_001218"
                     /pseudo
     CDS             complement(10493..10718)
                     /locus_tag="VQU28_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            11566..12438
                     /locus_tag="VQU28_001219"
     CDS             11566..12438
                     /locus_tag="VQU28_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HET2476578.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(12584..12928)
                     /locus_tag="VQU28_001220"
                     /pseudo
     CDS             complement(12584..12928)
                     /locus_tag="VQU28_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            13071..13361
                     /locus_tag="VQU28_001221"
     CDS             13071..13361
                     /locus_tag="VQU28_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476579.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKNAKNCVRLSQLSLNKR"
     gene            13385..14101
                     /locus_tag="VQU28_001222"
     CDS             13385..14101
                     /locus_tag="VQU28_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2476580.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSIGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIISDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(14230..15045)
                     /locus_tag="VQU28_001223"
     CDS             complement(14230..15045)
                     /locus_tag="VQU28_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HET2476581.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(15042..15947)
                     /locus_tag="VQU28_001224"
     CDS             complement(15042..15947)
                     /locus_tag="VQU28_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HET2476582.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGMLFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLVYSLLIGVIL"
     gene            complement(16218..16301)
                     /locus_tag="VQU28_001225"
                     /pseudo
     CDS             complement(16218..16301)
                     /locus_tag="VQU28_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            16345..18474
                     /locus_tag="VQU28_001226"
     CDS             16345..18474
                     /locus_tag="VQU28_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HET2476583.1"
                     /translation="MDKKYEKISQDLGVMLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRAQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYTAVKELFKRL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVSVGD
                     LVTVWVKKIDTEREKVNLSLLAPNETD"
     gene            18461..18910
                     /locus_tag="VQU28_001227"
     CDS             18461..18910
                     /locus_tag="VQU28_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HET2476584.1"
                     /translation="MKLTDYVKQVSLEDFGRAFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQKLGMEVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPLKLYRCQSCQQRYQRKRRIDTKRYRCGLCRGKLLLINQPED"
     gene            18969..19241
                     /locus_tag="VQU28_001228"
     CDS             18969..19241
                     /locus_tag="VQU28_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2476585.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(19595..19786)
                     /locus_tag="VQU28_001229"
     CDS             complement(19595..19786)
                     /locus_tag="VQU28_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476586.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            20214..20972
                     /locus_tag="VQU28_001230"
     CDS             20214..20972
                     /locus_tag="VQU28_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476587.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVVTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            20974..22962
                     /locus_tag="VQU28_001231"
     CDS             20974..22962
                     /locus_tag="VQU28_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476588.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQTKVVITVLV
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSSESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNEGVKEQKALTLNDHQFSVKEEFTKDFIVNHVPNQF
                     NILTADYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVNASEAEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFLHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(23004..23648)
                     /locus_tag="VQU28_001232"
     CDS             complement(23004..23648)
                     /locus_tag="VQU28_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HET2476589.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATTNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQNEEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
BASE COUNT         7272 a         4265 c         5013 g         7177 t
ORIGIN      
        1 gagaattcta gtaggcatac cagtcgtttc aagataagga attttagaag gtttttgaaa
       61 gtcatatttc ttcaattggt ttccgcactc agggcaagat ggggcgtcgt agtccagttt
      121 ggcgatgatt tccttgtgtg tatccttatt gatgatgtct aaaatctgga tattagggtc
      181 tttaatgtct agtaattttg tgataaaatg taattgttcc atatgaatct ttctaatgag
      241 ttgttttgtc gcttttcatt ataggtcata tgggactttt tttctacaat aaaataggct
      301 ccataatatc tatagtggat ttacccacta caaatattat agaacccttt tttattgaca
      361 gtatcaggat atctttttca aatttttggt ttgttagata atgaaaatgt ttctatcaat
      421 ctagatttag gattaataaa tcgtaaatgt aattataaag aaaaagataa gttaaaaaaa
      481 gatgtattag caagagttgg tctaactcat attcatctat aacaaaagat atatgaattg
      541 tctggcggtg aaacaaaacg tgttgcttta gcgaaaatca ttcttaaaaa tccaccatta
      601 atattcgcgg atgagttgac tgcatcactc gattcgacga cttcacaagg aattatgaaa
      661 ttgttattag gtatgaaaaa taaaaatcgt ctaatcatca ttgcaactca taatcccgat
      721 atttggaatc aagcagatga agttataaat ttaaatcaat tattacagtc aatatatatc
      781 ttctatattt taaagtaatt ttatctgaat ccgttaagag actattgtcc caggctcttt
      841 ctgatgttaa agactttcta ccaggttttt taaaagcata attgttagtt gtagtcattt
      901 attattcttc aaagaaaaat ggtggggcga attttttcag ttcttcaaag cacttttgag
      961 cagtatctgc atcttcacag atgataagac agacatcatt accacaaagg gtagcgatag
     1021 cgtcagggaa gctcaaagta tcaatgatag aaccaaagga ttgagccagt ccaggaaggg
     1081 tttttagtag gacttggtgt tgaactgggc gcatccagac aagggcgtct tccatgtaga
     1141 gttcgagacg tttttcccat tttgagatgg aaccattgtt aagaacataa taagcgctat
     1201 cttcttcgcg gacttttgat aggttcatat ttttgatgtc gcgtgagagg gttgcctgag
     1261 ttacttgaat gtcgttctca gcaagaaggg tttgcaactc agcctgtgta tgaatcttgt
     1321 tttttgtgat aagagcgcgt ataagttggt ggcggtgttc tgatttattc ataataatgt
     1381 aactcctttt agcaaggtaa ggtaagcatg gactgagcga ggtcgacagt caagtggtag
     1441 tctgtattgt cacggatggt gatttcaaag tcagtagtat agaggactaa acggagagtg
     1501 tctccttctt ttagcttgta aatagttggc tgcagttcaa attgaacgtc catccattca
     1561 tctgcagtaa tatcctctac taacagtaaa tcatttctat tttgtaaatt aaggtaacct
     1621 tttgtcacga ctctttgtgc ctctggtcta aatggcaatt cacagagatt ttccaacatg
     1681 tgatagcgac cgttgtcaat ggttctagca cttaaaatag ctggataagg ttgtaggtat
     1741 ttcttttgcc caaattctag cagttgggca gataagagcc ccttgtttgt actggatttg
     1801 atacgaagat tgagctgagc gcgaccgttt aggtggagat ctttagtcac aggaaggtta
     1861 atagtaatct gattagcttt cccttgatag agctctgtat tgaaggtttg gtatgtctta
     1921 ccatagcgct caaaatcctt atctgggtac tggttttgaa tagcttgctc ttcttgacca
     1981 agtgagaagg tttcacagtt ttcttgccca ccgaagttat caagtgataa ccaagtctgt
     2041 ggagctgtat tgtcctgcca gataacagta ggaagttgaa agtctgtttc ctgtcctagt
     2101 aatttcttgg tcaataaggc atttatggac tcacggaagt caattgattg ccaattgttc
     2161 atgtaaacat gggcaccatt atggaaaaag agatgcttgt gtatatgagt aggaagagca
     2221 tggaacatct ggtaaacatg aagtggtttg acattccaat cctgagaacc atgagtaaag
     2281 acaacctctg cctttacttt atgggcattg agcagataat tgcggtcatg ccaaaactga
     2341 ttgtagtccc cagtttttcg gtctagttga gctttcactt tttctaagtc agcttggtga
     2401 gcttcattgc cacggatata gtcgctagcc aagagattac gagaataggt taactcagca
     2461 agggagtcaa agtcctcacc tggataacca cctgggctag tcaccagacc gttttcacgg
     2521 tagtagttgt accatgatga aattcctgcc tcggcaatga taacttctaa accatcgact
     2581 cctgtagtcg caagaccatt ggacatggta cctagatagg aaagtcccgt tgtagcaact
     2641 tttccgtttg accaatcagc cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg
     2701 caacggccgt taagccaatc gatgacattt ttataagcct cgatttgctg gtagtctcca
     2761 ttagtcatga aacctgtcga gtctttggta ccaacacctg agacatagag attggcaaag
     2821 cctctcggaa ggaagtagtc gtttagtgta tagctagagt tgatgtgagt tagcttttcc
     2881 tcagcctctg ctataagctc agctttacct tggggttgga cgagatttag ttgaggtttc
     2941 tctagctcaa tcttgtgagg aagtttaacc tcaagctcgc cctccatctt gtagagagcc
     3001 ttgtcactag ccttgtcatt ggttccctga tgataagggc tggctgtcat gatggcaggg
     3061 atttttccat caaaacgagg gcgaataatg ctaaccttta ctaggtctga tagccctttt
     3121 tggtcagtat cgacacgaga ctcaacgtaa acgacttcac gaatgacatc ctggttagaa
     3181 aaagtagcca aactcttgcc gttaaagtag tggtagtcat tatcctccgg aataagacca
     3241 tcactaacaa gttggtcgat aagagtattt ccttttttgg tgcgagtatt gagtaactga
     3301 tagagatttt caatcaagtc accatatata atgggaaatc cagtttcttt acgaaaaacg
     3361 tcactatctt cgaagtcaac caaataagaa aagcctaaaa gttgaaaagc aacagtataa
     3421 aaaatatctg ctgtcagttc atcttctgat tgaaaaaatg tcagcaggtc tgttttttta
     3481 tcagctgcta ggatagaaag tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt
     3541 aaaaaggttt caagtgagtc tttgtgattg gctgtatttt gtaaatcaaa gccacatttt
     3601 tttagttcag ataagacatt ttcttttgga aaattgatat aactatattg attaaaacgc
     3661 atagaacctc catatagaat gacagttaag gttattatat caaaaaaaaa gcagaaaggg
     3721 aattgttaac ttcaaaagga aataatccaa taaaaatgaa taaagtacta aattcaatat
     3781 agagaacaga gtaacaataa gaataaatag atagggtata aaagttctag gagatttata
     3841 ttatatgctt tctgttttta tatacaatat agtataaata taaaaatgat gacaaaaata
     3901 caaatgaata gaaaataaat tagtaagctg atgaaatttt tctcaagaga agccatttat
     3961 aggtgaaaat ggtataatat agtgagaagg atagaggaga agtgtaaatt gatcgcacaa
     4021 ctagatacaa aaacagtcta tagttttatg gaaagcgtca tttcgatcga aaagtatgtg
     4081 agagcagcta aagaatacgg ctacactcat ttggctatga tggatattga caatctttat
     4141 ggcgctttcg actttctaga gattacaaaa aaatacggca ttcatccttt gctagggctt
     4201 gaaatgacag tgtttgtaga tgatcaggga gtgaatttgc gctttttagc tctatctagt
     4261 gtgggctatc agcagttgat gaagctttcg acagccaaga tgcaggggga gaaaacttgg
     4321 tcagtcctgt cccagtacct ggaggatatc gcggtcattg tgccttattt tgatagagtt
     4381 gagtcgttag aactaggctg tgattactat ataggggttt atccagaaac actagcaagc
     4441 gaatttcatc atcctatctt acctctttat cgggtcaacg cttttgaaag cagggataga
     4501 gaagttcttc aagttttaac agcgattaaa gaaaatctac cgctcagaga agttcccttg
     4561 cgttcgagac aagatgtctt tatatcagca agttctttag agaaactatt ccaagagcgt
     4621 tttccgcaag ctttggacaa tttagaaaag cttatttcag gcatttctta cgacttggat
     4681 actagtctga aactgcctcg ttttaatcca gctagaccag cagtagagga gttgagagag
     4741 cgtgctgaac tggggcttgt tcagaagggg ttgactagta aagaatatca agatagacta
     4801 gaccaagaat tgtctgttat tcatgatatg ggctttgatg attatttctt ggttgtttgg
     4861 gatttgttgc gttttggacg atcgaatggc tattatatgg gaatgggaag gggttctgca
     4921 gtaggcagtt tggtttctta tgccttagac atcacgggga ttgacccagt agagaaaaat
     4981 ctgatttttg aacgctttct taatcgtgaa cgctatacca tgcctgatat tgatattgat
     5041 atcccagata tttatcgtcc agattttatc agatatgttg gtaataaata tggtagtaaa
     5101 catgcggcac aaatcgttac tttttcaacc tttggagcca agcaagctct tcgagatgtc
     5161 ttgaaacgct ttggtgtgcc agagtatgaa ttatctgcaa ttactaagaa aatcagtttt
     5221 cgtgacaatc ttaagtcggc ctatgagggc aatctccagt ttcgtcagca aatcaatagt
     5281 aagttagaat accaaaaagc ttttgagatt gcttgcaaga tagagggcta tccaaggcaa
     5341 acctctgtcc atgcggctgg tgttgtaatt agtgaccaag atttaaccaa ctacattcct
     5401 ctaaagtatg gtgatgaaat tccactgact cagtatgatg ctcatggagt tgaggctagc
     5461 ggacttttga agatggactt tctgggacta cgaaatttga cctttgtcca gaagatgcaa
     5521 gagttgcttg ctgaaacaga aggtattcat ctgaaaattg aagaaatcga tttagaagac
     5581 aaagaaacat tagctttatt tgcctctggt aatacaaaag gtatctttca atttgagcaa
     5641 ccaggtgcca ttcgtctgct taagcgtgtg caaccagtct gttttgaaga tgtcgtcgcg
     5701 actacttctc taaatcgacc gggtgctagt gactatatca ataattttgt ggcaagaaag
     5761 catgggcagg aagaagtgac tgttctggat ccagtactgg aggatatttt ggctccaacc
     5821 tacggcataa tgctctatca ggagcaggtt atgcaggttg cccagcgact tgccggattt
     5881 agtcttggga aagccgatat tttgcgtcgg gctatgggga aaaaggatgc ctctgccatg
     5941 catgagatga gggcttcctt tattcaaggt tcattagaag ctggtcatac tgtggaaaaa
     6001 gcagagcagg tctttgatgt tatggagaag tttgcaggtt atggttttaa caggtcacac
     6061 gcctatgcct actcagcctt ggccttccag ttggcttatt tcaaaacgca ttatccagcc
     6121 attttttatc aggtcatgtt aaattcttcc aacagtgatt acttaataga tgcacttgaa
     6181 gcaggttttg aagtagcctc tctatccatc aacaccattc cctatcacga taaaattgcc
     6241 aacaaggcca tctatctagg tttgaaatcc attaaaggag tcagtaatga tttagctctc
     6301 tggattattg aaaatagacc ttattctaac attgaagatt ttatagctaa attacctgag
     6361 aattatctga aacttcctct gctagaacct ttggtaaaag ttggtctttt cgattcattt
     6421 gaaaaaaatc gtcaaaaagt atttaataac ttagctaatc tatttgaatt tgtgaaagag
     6481 ttgggaagtt tgtttggaga tgctatttat agttggcagg aatcggaaga ttggacggaa
     6541 caagaaaaat tttatatgga acaagagctt ttagggatag gtgtcagcaa acatccacta
     6601 caagctattg caagtaaggc tatttacccg attaccccaa tcggaaattt gtcagaaaat
     6661 agctatgcta ttatcttggt tgaagttcag aaaataaaag tgattcgtac caaaaagggt
     6721 gaaaatatgg ccttcttaca ggcagatgat agtaagaaaa aattggatgt tacactcttc
     6781 tcagatttat atcgacaggt tggtcaggaa gtaagagaag gagcattcta ctatatcaaa
     6841 gggaaagtcc aatcacgtga cggtcgtctg caaatgattg cacaagaaat aagagaagca
     6901 gttgctgaac gcttttggat acaggtgaaa aatcatgaat cggatcaaga aatttcacgt
     6961 attttagaac aatttaaagg cccaatccca gtcatcatcc ggtatgaaga ggaacagaaa
     7021 accatcgttt ctccccatca ttttgtagct aaatctgatg aattagaggc gaaattgaat
     7081 gaaatcgtta tgaaaacgat ttatcgctaa aaatacggaa aatagaagaa ttttaaaatc
     7141 aaatgtgata taatcaagaa gaatgttaaa agaaaaagga gcataaccaa tatgaaacgt
     7201 attgctgttt tgactagtgg tggagacgcc cctggtatga acgctgccat ccgtgcagtt
     7261 gttcgtcaag caatttcaga aggaatggaa gtgtttggta tctatgacgg atatgctggt
     7321 atggttgccg gtgaaattca tcccctagat gcagcttcag taggggacat catttctcgt
     7381 ggtggtactt tccttcactc agctcgttac ccagagttcg ctcaacttga agggcaactt
     7441 aaagggattg agcaattgaa aaaacacgga attgaaggtg tagttgttat cggtggtgac
     7501 ggatcttacc acggcgctat gcgtttgact gaacatggct tcccagctat tggtcttcca
     7561 ggtacaatcg ataacgatat cgttggtact gactttacaa tcggttttga cacagcggtt
     7621 actactgcca tggacgctat cgataagatt cgtgatacat catcaagtca ccgtcgtact
     7681 tttgtaatcg aagttatggg acgtaacgct ggtgatatcg ctctttgggc tggtattgca
     7741 actggtgctg atgaaatcat catccctgaa gcaggcttca agatggaaga tatcgtagca
     7801 agcatcaaag ctggttatga atgtggtaaa aaacacaata tcatcgtctt agctgaaggt
     7861 gtgatgtcag cggctgaatt tggtcaaaaa cttaaagaag ctggagatac aagcgacctt
     7921 cgtgtaacag aacttggaca tattcaacgt ggtggttctc caactgcgcg tgaccgtgtt
     7981 ttggcgtcac gtatgggtgc acatgctgtt aaacttctta aagaaggtat cggtggtgtt
     8041 gcggttggta ttcgtaacga aaaaatggtt gaaaatccaa ttcttggtac tgcagaagaa
     8101 ggggcattgt ttagccttac tgcagaaggt aagattgtgg ttaacaaccc gcacaaagcc
     8161 gatattgagc tatctagctt gaataagagc ttgtcataat ttacaattta gttaataaga
     8221 ccaaaaaggt catatataaa ggagtcacaa aaatcatgaa caaacgtgta aagatcgttg
     8281 caactttggg tcctgcggta gaaatccgtg gtggtaaaaa attcggtgag gacggatact
     8341 ggggtgaaaa acttgatgtt gaagcttcag ctaaaaacat tgctaaattg attgaagctg
     8401 gtgctaacac attccgattc aacttctcac acggcgacca ccaagaacaa ggtgagcgta
     8461 tggcaactgt taaacttgcg gaaaaaattg caggtaaaaa agttggtttc cttcttgata
     8521 caaaaggacc tgaaatccgt acagaattgt tcgaaggtga agctaaagaa tattcataca
     8581 aaactggtga aaaaattcgt gttgcaacta aacaaggaat caaatcaact cgtgaagtga
     8641 ttgcgttgaa cgttgctggt gctcttgata tctatgatga tgttgaagtt ggtcgtcaag
     8701 ttttggttga cgatggtaaa cttggtcttc gtgtggttgc taaagatgat gcaactcgtg
     8761 aatttgaagt tgaagttgaa aacgatggta tcatcgctaa acaaaaaggt gtgaacatcc
     8821 ctaacactaa aattcctttc ccagctcttg ctgaacgcga taacgacgat atccgtttcg
     8881 gtcttgaaca aggtatcaac ttcatcgcaa tttcattcgt acgtactgca aaagatgtga
     8941 acgaagttcg tgcaatctgt gaagaaactg gaaacggaca tgttcaatta ttcgctaaaa
     9001 tcgaaaacca acaaggtatc gataacttag acgaaatcat cgaagcagct gatggtatta
     9061 tgattgctcg tggtgatatg ggtatcgaag taccgttcga aatggttcca gtttatcaaa
     9121 aaatgattat caagaaagtc aatgctgcag gtaaagttgt tatcactgca acaaacatgc
     9181 ttgaaacaat gactgaaaaa ccacgtgcaa ctcgttcaga agtatcagac gtattcaacg
     9241 ctgttatcga cggaactgac gctacaatgc tgtcaggcga gtctgcaaac ggtaaatacc
     9301 cactcgagtc agtaactaca atggctacaa tcgacaagaa tgctcaagct cttcttaatg
     9361 aatacggacg tcttgattca gattcatttg agcgtaactc taagacagaa gtaatggctt
     9421 ctgctgttaa agatgctact agctcaatgg atatcaaatt ggtcgtaact cttactaaga
     9481 caggtcatac tgcacgtttg atttctaaat accgtccaaa tgctgacatc ttagcattga
     9541 catttgacga attgacagaa cgtggcttga tgttgaactg gggtgttatc ccaatgttga
     9601 cagatgctcc atcttcaact gacgatatgt tcgaaatcgc tgaacgtaaa gcggtagaag
     9661 caggtctcgt tgagtcaggc gatgatatcg ttatcgttgc tggtgtgcca gtaggagaag
     9721 ctgttcgcac aaacacaatg cgtatccgca cagtacgtta agaaaaatat aaaaacctat
     9781 catatccagc tttagagctt gtgtgatagg ctttttgtat agagggtaag aaataggcaa
     9841 aactttcata atggattgat actcttcgaa aatcaaattc aaaccacgtc agcgtcgcct
     9901 taccgtatat atgttactga cttcgtcagt tctatctaca acctcaaagc agtgctttga
     9961 gcaacctgcg gctagcttcc tagtttgctc tttgattttc attgagtatg aaataagata
    10021 tgcacaaatt gattaggaaa ttcaaatgaa tttctacaaa tgttttagca atcgtaatgt
    10081 acttgtctag attcgatctg atatattttc gatttaatga tatggtattt aaaacctcca
    10141 aagtagcttg ctccattctt ttacttacgt gagtgtagat gttatttact gttttagcgt
    10201 ttttgtgttc cactctaacc attatagcat tcttctcagc tagtgtacta aggagtgtgt
    10261 gcctgaaaat atgggaacta aggggctggt ttatcggttt ctctagttta gtatttgcct
    10321 tttgcaaagt gatgttaaat gcctttctct aaatttacat atcactattg tttaacaaaa
    10381 tctaatctac tttaggtcac ttattctttt tttgaaatgt agaatgaact ttttcaaagt
    10441 ttttcgaatc ttttaaaatc tgtttgtttt atatcgccat tctccccctt ttttaattct
    10501 ccctatatag cctgacaact ttcccgatgg tacgaatatg gttgctttcg tctaggtgga
    10561 tgtcggggta tttgggattg agttttttta aggtagcctt ggcggagttt cttgacatag
    10621 ttagcgccgt ctacttggaa gatgccgatg gtattatagt caatctgtgg ggtattcttg
    10681 ataaataggt agtcgctgtt tcttatcttt ggctccatgg acttgctgac gacataagcg
    10741 attgggtcgt agtcgtctgg gataatggaa actccataac taaatcgtcg tcctgcatcg
    10801 agcggctacc tgcagagata aactacctaa cacgagagta agtagtctgt ctgtagtcgt
    10861 ccagtctgat gatttttacg atatttcgtt tttctgctca tacagttgcc tctcggcata
    10921 ggtcagaact ttaccttgtc tgggtggtgc ccgttggtcg tagatagatt ggatatcgct
    10981 aggagaatcc ttttgaactg gaggaaagag ggtatcaatc aagctactga atactttagc
    11041 taagtcaaat atagtatttt tcttagtaga cctaaccctt ttttcataat ttccaatggt
    11101 gtttttactt ataccaatct tagtacccaa ttcttattga gtccaaccat tactagtcta
    11161 tattgtttta tagttgattg agtttggaat agtacgctgt agctgctaaa acatttctag
    11221 gaattaattt gactttccta atagagttgt tcatatctta tttcaatcta ctatgttttt
    11281 cacctctaac aatcgcaatc tcttctttat ccatgaatga aatcgctttc tatttttgta
    11341 agtaaagcat cacacgaaat ccacgaaaat gaaaaccttt gttgtgtttt cgtaaaaaat
    11401 ttgttgacag agcacgaaac gcctactata attaaatcaa gcttaaggaa ataacaaaaa
    11461 aacaaatcgg agggaaagac gatgaacaca ttaaatgaga aagtaatcaa tatctgtaaa
    11521 gcagtagtta aagaaacttt aatccaagac atttaggaga aagaaatgaa gaaatcaaga
    11581 aaattagcca cattaggaat ctgttcagcc ttgtttttag gcttggctgc ttgtcaacaa
    11641 caacatgcta cttctgaggg gacgaatcaa aggcaaagca gttcagcgaa agttccatgg
    11701 aaagcttcat acaccaacct aaacaaccag gtaagtacag aagaggtcaa atctctctta
    11761 tcagctcact tggatccaaa tagtgttgat gcatttttta atctcgttaa tgactataat
    11821 accattgtcg gctcaactgg cttatcagga gatttcactt cctttactca caccgaatac
    11881 gatgttgaga aaatcagtca tctctggaat caaaagaagg gcgatttcgt tgggaccaac
    11941 tgccgtatca atagttattg tcttttgaaa aattcagtca ccattccaaa gcttgaaaag
    12001 aatgaccaat tgcttttcct agataacgat gcgattgata aaggaaaggt ctttgattca
    12061 caagataagg aagagtttga tattctattt tcgagagttc caactgaggc aactacagat
    12121 gtcaaggttc acgctgaaaa gatggaaaca ttcttctcac aatttcaatt caatgaaaaa
    12181 gctcgaatgc tgtctgtagt cttgcacgac aatctggatg gcgagtatct gtttgtaggc
    12241 catgttgggg tcttagtacc tgctgatgac ggtttcttat ttgtagagaa attgactttc
    12301 gaagagccct accaagcgat taaatttgct agtaaggaag attgctacaa gtatttgggc
    12361 accaagtatg cggattatac aggcgaggga ctggctaagc cttttatcat ggataatgat
    12421 aagtgggtta aactttaata gtgttatgat atgacagtaa tcttaggatt tttatttcta
    12481 tttttgatga cttggtgggt tatttcaatc gttaatagcc agaaaccacg caaaaatgaa
    12541 acatttattg gctatatcca aagatacgat attgatggga atggctatca atcattaccg
    12601 tgtcctcaga actgagagga gttcttgaaa ccgtaaaacc gctttgaaca agagttactt
    12661 caacccattg gctccgacgg agtaagttgc tttcgtgaat accaaaatca gccgcaattt
    12721 gttcataagt tcgatattct cgcacatatt gaagagtggc cataagaagg tcttctaggc
    12781 ttaatttagg ttttcgtcca ccttttgcgt gtttaagttg ataagctgtt tttaatacag
    12841 ctaacatctc ttcaaaagtg gtacgctgaa caccaacaag acgcttaaat cgtgcatcag
    12901 ttagttgttt acttgcttca taattcatag aactactata ccatattttg tttcgcagga
    12961 agtctaatgc taaaatctta tcagtgagaa gatttcttaa agtttattct ttgtagtttc
    13021 atctgtatta gtacataatt taacttagag aaaataggag gactaatcga atggaattaa
    13081 aagattttac agaaaaagaa caggaaatga ttaagaaaag gcttacaatg tctaatatta
    13141 gtgacaaaga aactactgag aagattctgg cgctagtccc acaagacttg attaagcgaa
    13201 tcccgttttt tgtcagaaaa catgctacaa cacgtacgat taaacgtatt tcaattgaat
    13261 accctgaact ctacgctgta gctcaaacaa gtggtgagat tccagaaaaa aacgcgaaga
    13321 attgcgtcag attatcacaa ctatctttga acaaaagatg aataagcata gtattaagta
    13381 gagaatgaaa aaatatttta ttggcggttt gggaagcaat gcctatcata gcaaggattt
    13441 tcttcaagaa ctagattcgc aggtctattt tctaaatcca tatgaaaagc atcttcgaga
    13501 tgaaacagaa ttgaaatcat ggtttaaaaa tgagattgta gaggaagaat ctatctgtct
    13561 gataggtcat tctattggag gcgatttagc tcgttatttc gcatcggaat ttgaagaagt
    13621 aaagaaactg attcttttgg atggtggcta tctagattta gataagattt tacctttgga
    13681 tacagagtta gaggaaacta aaaattatat caaatctcaa attatttcgg acttagatgt
    13741 tcttacttct aaagaaaaat ctgaagcaaa gcattggtca gaaaatatgg agaaagctgt
    13801 aagacagtcc tatcactgga atgttgagta taatagatat gagttggcta taaattatga
    13861 aaatatagaa gcgatactcc gcctacggag aaaaatacaa gcttttaaga gagaagtggg
    13921 agataccttg tttattagtc ctcgctatcc taatgaagct acatggagag aggaagccct
    13981 aaaagaattg ccagactatt ttgatactat ttttttagaa aactttggcc atgagcttta
    14041 tactcaagca cctaaagaaa tcgctagtct aatgaatgag tggctcgctt attttctatg
    14101 agctacttgt attgctggaa atggtataag atgaaaatct acaagtcact aattttcata
    14161 taacaaaaaa cgaacaggct ctttttctgt tattcagaag agaacttgtt cgtttttatc
    14221 ttgattttct taaaaagctc tatatacata gggattttct ggtttatcga ctttggtaaa
    14281 gctgtcgatt tccaaggttg gaaggttttg tttgagttct ttatggtagt gattgaccag
    14341 ttttcctttg gctgttatcc aagtgttgtt ttcatactgc cgggtattgc ccttggtcag
    14401 caatccatag acaccagaat ctgcgataca gtggataatg ccgaatcgga acagaaattg
    14461 actattggca tgactggggt cgttatagac aaagcctgta aactggattg tcttgccctc
    14521 aaactcatct ggatagtcgt agatagcctc catgacttcc atatagtttt cattagtgat
    14581 ctgaatacta tcttgggata agtatttatc cgccgccgtt cgcatttcct tttcataggc
    14641 tgattttgaa aaataagaac tggtatctgg tttcaaatat tggcttgtcg tcccttcgct
    14701 tgtctgaatg gctagatctg ttccttccga tagggggaaa tgataacctt tagcagaaac
    14761 ggtctgagaa tccaagctaa cagttgggaa agttaagccg atgacaagtg gaatagccag
    14821 aagagaaata ctcgtcatct tggccaatcg gctgttcaga tgactgtggg ttttgacttg
    14881 cttcatccag atatacaatt gaacaattgc caagataaag gaaagcacca tggagatata
    14941 ggccagatag gaatagtgca tgttgatgta ctggtttagt ttgcccgaca gatggaggta
    15001 aatagtcagt tcaaaatagc cagctaaaac taaaaatcga atcataggat gactccaatc
    15061 aagagagaat agactaagac gacaagagtt acaatcgtta tgaagtgact gataaatcgt
    15121 gctttcaagt aatttttcat catgagaata tttttgatat ccagcattgg accaatgacg
    15181 agaaaggcca gaactggtgc caaaccgaaa ctcgagagaa gagaagcacc tataaaggca
    15241 tccgcctcac tacagagcga aagaagaaag gccaaaatca tcaagagcag gatggcaaaa
    15301 agaggagtcg cactgataga ggtcagaatc cgagtcggaa cgtagacctg tattatagaa
    15361 gcaaagaggc agccaaatac caaataacgc cccgtatcaa aaaattcatc aatggcctgc
    15421 acaaagactt gaaaaacttt ttttgcagaa ctcaagtaag aaaaatcatg ctcatgacaa
    15481 gccagacgat tttctttctg aatcggttct tgccagaaaa atcctagaaa aattcctagt
    15541 attacagcca caagaatgga acccagagct cgtaataggg cgacatggaa ggagttgcca
    15601 aaggcagaat aggtcgcaaa aagaacaatg ggattgataa caggtgctgt cacaagaaaa
    15661 ggaacggctg tgtaactggg aacctttttt tccagaaaac gattgatgat ggggacgatt
    15721 ccacattcac aagaagggaa aagcataccg acaaaggtcc caaaaaagat tctcccccaa
    15781 cgatttcgag ggagaaaatg ataaaccttg tcaggtgtga tataaacttc aatcagccct
    15841 gagacaatgc ttcctattag aacgaaggga agggcttcaa tgataatgga gagaaaaatg
    15901 gctccagttt gaagtacact agatgggagt tgttgaaaag cagtcatcta gtttttcttt
    15961 tctgctttgg cttttttctc tttgtcatca gggaaagtaa cttgtttcaa atcagggagt
    16021 ttagcaaact cttcaaacat cttgtccaag tcgtctcgtt ttgtaaattt tacagccata
    16081 ggtgtacctc gattctaaag tctacctcta ttatactatt attttgcgga aaagggagtt
    16141 ttttaatttg ttttcgatag ttgatacact tcgaaaatct cttcaaacca cgtcagcttc
    16201 gccttaccgt atatatgtga cttatccaca acctcaaaac agtattttga gcagcctgcg
    16261 gctagcttcc tagtttgctt tttgattttc attgagtatg aaaaggattg gagaaagcct
    16321 tccagcttgt ggtataatag atttatggat aaaaaatatg aaaaaatctc tcaggatttg
    16381 ggagtgatgt taaagcaaat tgataccgtt ctaagtttga cagctgaagg ggcgactatt
    16441 ccctttatcg cgcgttatcg caaggacatg actggtagtc tggatgaggt ggcgattaag
    16501 gctattattg atttggataa aagtctgaca aatctcaatg accgtaagga agctgtctta
    16561 gctaagattc aagaacaagg taagttgacc aaggaattgg aagaagctat cttagttgcc
    16621 gaaaaattag cagacgttga agaactctat cttccttata aggaaaagcg tcgtaccaag
    16681 gcaaccattg cccgtgaagc tggactcttt cctcttgctc gtttgatttt gcagaatata
    16741 gttgacttag agaaagaagc tgaaaagttc gtctgtgaag gatttgcgac tggcaaggaa
    16801 gccttgaccg gtgcagttga tattttggtc gaagccttat cggaagatgt gaccttgcgt
    16861 tctatgactt atcaggaagt gctgagacac tctaaactta cttctcaagc caaggatgaa
    16921 agtcttgatg aaaagcaggt ttttcagatt tattatgatt tttcagagac agttggaact
    16981 atgcaaggct atcgtacctt ggctctcaat cgtggggaga aacttggtgt cttgaagatc
    17041 ggttttgaac atgcgacgga ccgtattctt gccttctttg ctactcgttt caaggtgaaa
    17101 aatgcttata ttgatgaagt tgttcagcaa tccgttaaga aaaaggtctt gcctgctatc
    17161 gagcgtcgta ttcggacaga attaactgag aaagctgaag aaggagctat ccaacttttt
    17221 tctgacaatc tgcgcaatct cctcttggtt gctccactga aagggcgcgt ggttcttgga
    17281 tttgacccag cctttcgtac aggtgccaag ttagctgtcg tggatgcaac aggaaaaatg
    17341 ctgacaactc aggttattta tcctgttaaa ccagcatcag ctcgtcaaat cgaagaagcc
    17401 aagaaagatt tagcagattt aattggtcaa tacggtgtag agattattgc cattggaaat
    17461 ggaacggcca gtcgtgaaag tgaagctttt gtagcggaag ttctgaaaga tttccctgaa
    17521 gtcagctatg ttatcgttaa tgaaagtggt gcttctgtct attctgccag cgaacttgct
    17581 cgtcaggagt ttccagactt gaccgttgaa aaacgctctg ccatttctat cgcccgtcgt
    17641 ttgcaagatc ctcttgcgga attggtcaaa atcgatccta agtcaattgg tgtcggtcaa
    17701 taccaacacg atgtcagtca gaagaaacta tctgagagtc tggactttgt tgtcgataca
    17761 gtggttaacc aagttggtgt caatgtcaat acagctagcc cagctcttct ttcacacgta
    17821 gctggactca acaaaactat ctctgaaaat attgtcaaat accgcgagga agaaggaaaa
    17881 atcacttcac gcgcccaaat caagaaagtt cctcgtctgg gagccaaggc ctttgagcag
    17941 gctgctggtt tccttcgtat ccctgaaagt agcaatatcc ttgataatac aggagttcac
    18001 ccagagaatt acactgcagt taaggaactc ttcaaacgtt tagatattaa agacctaaac
    18061 gaagaagccc aaagcaaact caagtccctt tcagtcaagg aaatggctca agagctagac
    18121 ctcggtccag aaacccttaa agatatcatt gcagatcttc tcaaaccagg tcgagatttc
    18181 cgtgattcct ttgacgcacc tgtgcttcgc caagatgtcc tagatatcaa agacctagtg
    18241 gtaggtcaga agctagaagg tgttgtgcgt aacgttgttg acttcggtgc cttcgttgat
    18301 atcgggattc acgaggacgg cttgattcat atttctcata tgagtcgcaa gtttatcaaa
    18361 caccctagtc aagtggtgtc agttggagat ttggtgaccg tttgggtcaa gaaaattgat
    18421 actgaacgtg aaaaagttaa tctgtcgctc ctcgctccaa atgaaactga ctgattatgt
    18481 caagcaggtt tcactagaag acttcgggag agcttttatc catcatgtcc agtggaatag
    18541 gcgtctacgt tcgacaggtg ggcgattttt ccccaaagat gggcatctgg attttaatcc
    18601 caaggtttat cagaaactgg gtatggaggt ttttaggaaa attgtccgac atgaactctg
    18661 tcattatcac ctttatttcc aagggaaggg ctatcaacac aaggatcggg attttaagga
    18721 actgttgaaa gcagtggatg gattaaggtt tgtaccatcc ttgcccaata gtaactctaa
    18781 accactcaag ctttatcgtt gtcaatcctg ccagcaaagg tatcagcgca agcgtaggat
    18841 tgataccaaa cgctatcgct gtggactttg tcgaggtaaa ttgcttctga taaatcagcc
    18901 tgaggactga tgaaagccga gcctccccgt gatatactat gtctaaccta gcagaaagag
    18961 gaaatatcat gaacagtaaa ttttataaaa tgagacgcaa tcgcatgatt tcaggagttc
    19021 tggcaggtct gtcagacaag tggaattttg atgttaccct agttcgtttc ttgtttgcca
    19081 tttttacagt tgcaaacttt ggacttggcg tgattatcta tatcatccta gcttctatca
    19141 tgcctaccaa ggaagaaatc gaagcagaaa tgtacggaac aggcccacgt aaacgcaaag
    19201 aagcccaagc cattgacgac aatgaaggct ggttttggtg aggttctaca gaagatttgg
    19261 aataagcaaa aagctttaaa tcaatagtct aaactgcttg atttaaagct tttttacttt
    19321 tatactcaat aaaaatcaaa gtgcaaacta ggaagccagc cacaggctgt aatttagtcg
    19381 gcaatgtgaa gttgacatag tttgcacttt gatttttgaa tagtattaat aatatttcgc
    19441 cagcttcatc cacaaactat agtggattgg aactagaata gtacacctct acttctaaaa
    19501 cattgttaga aatcgatttg actatcctga tcgatttgtc ctgttcttat ttcattttat
    19561 tataaaagca gtaatttaaa ctcagttttt taatctattt ttgctgctga gacaatacat
    19621 ttttggaaaa atggtggatg ctgataacaa ctgataacca gcaaataaca taagcagtgc
    19681 cggtaaattc tgtaccgata aagtgtgtgt gttcaactaa gagctgaccg atgaatggag
    19741 agagcagata gagaagtccg atgagtatat gttctttgtg tttcattaaa gcgcaccttc
    19801 tttccttata ggttgaacga cttgcaagtg ttaaattgaa tggtatgtta tctaggtaag
    19861 cttactcaaa tgatagttcc cgcttcctcc tttccaaagt aagccttttc atacttatat
    19921 tttacaactt taaaaatagg aaatcaaatc attttctaag taattttgct tatatttcac
    19981 ccaatcttac aaaaatgtaa gattaaagtc tcttttgtaa ggttaggtta tggcaagcgg
    20041 tctttttttg atgtaaacta gactcataaa gaacaaagga gtaacatcat gaaacaaatc
    20101 ttacataaga aaactagaaa accaagtcac aaagaaattg agcgcgttca gctgggatgc
    20161 actatgatgc aagctacatt tcatttgatg ggatattaag aaaggagatt ctcatgacac
    20221 ttttagatgt aaaacacgtt caaaaaattt ataaaacacg ttttcagggc aaccaagtag
    20281 aagccctcaa ggatattcac tttaccgtcg aaaagggtga ctacgttgcc atcatgggtg
    20341 agtctggttc tggtaaatca actcttctca atattctagc tatgctggat aaaccaagtc
    20401 gtggtcaggt ttacttgaat ggaactgaca ccgcaaccat taaaaattca caggcctcta
    20461 gcttccgtcg tgaaaagttg ggctttgtct tccaagactt taacttgcta gatactctat
    20521 ctgtaaagga caatatcttg cttccgcttg tcttgtcaag aagacctatt acagagatga
    20581 tgaagaaatt ggtggtgaca gctgagaatc tgggaataaa ccaattacaa gagaagtacc
    20641 cttacgagat ttctggtggc cagaaacagc gtgtagcagt agcacgcgcc atcatcactg
    20701 aacctgaaat tctccttgcg gacgaaccga caggagctct tgactccaag tcatctgcag
    20761 ccttacttga tgtctttgat gaaatcaatg agcgcggcca aaccattctc atggtaaccc
    20821 actcaacagc agcagctagc agagctaaac gtgtactctt tatcaaagac ggcattcttt
    20881 acaatcaaat ctaccgtgga gagaagacag aacgtcagat gttccaagaa atctctgata
    20941 ccttgactgt catggcaagc gaggtgaatt agtatgtttc gattaaccaa taagttagcg
    21001 gtatcgaact tgattaaaaa ccgcaaactc tactatcctt ttgcgctggc tgttctcttg
    21061 gcagtcactg tcacctatct cttttactct ctaaccttca atcccaagat tgctgaaatt
    21121 cgtggaggaa caaccattca ggctacactt ggatttggta tgtttgtcgt cacccttgcg
    21181 tcagccatta tcgttctcta tgccaatagt tttgtcatga agaaccgttc caaggaacta
    21241 ggaatttatg gcatgttggg cttggagaag cgtcatctta tcagtatgac ctttaaggag
    21301 ttagtggtat ttgggattct aactgttgga gcgggtatcg gtattggagc cttgtttgac
    21361 aagttaattt tcgctttcct gctcaaacta atgaaattga aggttgagct ggttgctacc
    21421 ttccagacga aagttgtcat tacagtgctt gttgtcttcg gtttgatttt cctaggcctc
    21481 atgttcctga atgcccttcg aatcgcccgt atgaatgccc tccagctctc tcgtgagaaa
    21541 gctagtggag agaaaaaagg tcgcttcctt cctctccaaa ccattcttgg ttccataagt
    21601 ttaggaattg gctattatct tgcccttacg gtaaaagatc ctcttacagc cttaacaacc
    21661 ttcttcatag ctgttttact ggttatcttt gggacttatc tcttgtttaa tgcagggatt
    21721 accgttttcc tccaaatctt aaagaaaaat aagaaatact attaccaacc aaataatctc
    21781 atatctgttt ctaacttgat tttccgtatg aagaaaaatg cagttggact agccaccatc
    21841 gctatcttgt caacaatggt tttggtaacc atgtcagcag cgacaagcat tttcaattcc
    21901 tcagaaagct ttaaaaaagt tctaaatcct catgattttg gggtttcagg gcaaaatgtt
    21961 gaaaaagaag acttggacaa actcttgagc cagtttgcaa gtgacaaagg ttatagtgtc
    22021 aaagagaaag aagtacttcg ttacagtaac tttggtattg caaatcaaga aggaactaag
    22081 ttaactattt ttgaaaaagg acaaaaccgt gtccaaccaa aaacagtttt catggtattt
    22141 gaccaaaaag attatgaaaa tatgactggt caaaaactgt ctctatcagg aaatgaggtc
    22201 ggactctttg caaagaatga gggagttaaa gaacagaaag ctctaactct aaatgatcat
    22261 caattttctg taaaagaaga atttactaaa gattttattg tcaaccatgt tccaaatcag
    22321 tttaatattt tgactgctga ttacaattac cttgttgtac ctgatttaca agcctttttg
    22381 gatcaattcc cagattcggc tatctataat cagttttacg gtggtatgaa tgtaaatgcc
    22441 agtgaagcag aacaactcaa ggtcgctgag gagtatgaaa aatacttaca aaagtttaat
    22501 gctcaattaa acactgaagg taactatgtg tatggtagca ctctagcaga tgctagtgct
    22561 cagatgagtg ccctctttgg tggtgtcttc tttatcggta ttttcttatc cattatcttt
    22621 atggtcggaa ccgttctggt catctactac aaacaaattt ctgaaggata tgaagaccgt
    22681 gagcgcttta ttatcttgca gaaagtcggt ttagatcaaa agcaaatcaa gcaaaccatc
    22741 aacaaacagg ttttaactgt attcttcctt cctttgctct ttgccttcct acatctagcc
    22801 tttgcctacc atatgcttag tctgatttta aaagtgattg gtgtactgga tacgactatg
    22861 atgttgattg tgaccttgtc tatctgcgct atcttcctca tcgcctatgt gctgattttc
    22921 atgattactt caagaagtta tcgcaagatt gtgcaaatgt aaaaaagata cctcgacttc
    22981 aaaatcgagg tatttcttgt attctaaatg ctgaaaagtt gttcgagcag gaaggtaact
    23041 cccatggtca agagaccaat agcaaggttc cgaatcatag ctgttttggt tggggctttt
    23101 ccaagtctag cacttgtgta accagtgaga agaagggcca caccgacaat aaggacggta
    23161 gcagggatgc ggtaatcact tggaaaaatg gtcactgaca gcattggagg caaacttcta
    23221 aggaaaaagg caacgaagct agaaatggca gcgtgccaag gattggtaaa ttcttcatac
    23281 tcaatcccat atttttcctc taccagagcc ttgagtggat ttttaagaaa gatcttatta
    23341 gtcaagagtt gggcagaagt tttgaattct tcattttgga tataagcagc atagagggat
    23401 tttttggcta gttccctatc ttggtctagc aagagttttt ctcgcgaaac ggcagcttcc
    23461 tcggtatctt ttggagttga aacggataca tattctccac cagccattga aaaggcacca
    23521 gctaagatag ccgcaaatcc tgataaaaag ataatccaga tattggtcgt ggcactggca
    23581 accccgataa ccacaccagc aatggaaata attccatcgt tagcatcaag aacacccgca
    23641 cgcaggatat ttaaacgacc tgcaaaattt gaatcaattt cgtgatttgt ttctgacgct
    23701 aaatttcaag ttcaagttag ccatcca
//
LOCUS       DAVBCB010000032        23312 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVBCB010000032 DAVBCB010000000
VERSION     DAVBCB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.032"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(196..654)
                     /locus_tag="VQU28_001233"
     CDS             complement(196..654)
                     /locus_tag="VQU28_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HET2476590.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(651..1091)
                     /locus_tag="VQU28_001234"
     CDS             complement(651..1091)
                     /locus_tag="VQU28_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476591.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1675..2541
                     /locus_tag="VQU28_001235"
     CDS             1675..2541
                     /locus_tag="VQU28_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476592.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYQVNHLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAYFLYKLEENMSMT
                     LKVEHYIADITE"
     gene            2878..3435
                     /locus_tag="VQU28_001236"
     CDS             2878..3435
                     /locus_tag="VQU28_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase family
                     flavoprotein"
                     /protein_id="HET2476593.1"
                     /translation="MDKKNLLVLVTGSVGASNVDTYLYYIKKFYNVKVILSENSKKFI
                     SKELISYFCDKVYDEIFVEEVVPHVFLPYESDLLLILPATANVIGKIANGIADDLVTA
                     TVLNFNKKIIFCPNMNSTMWDNHIVQRNVSILKELGHIFLFESKKTYEVGLRKAIDST
                     CSMLQPQSLVKELIKLENIVLEEGH"
     gene            3656..4762
                     /locus_tag="VQU28_001237"
     CDS             3656..4762
                     /locus_tag="VQU28_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HET2476594.1"
                     /translation="MINKKIQQVVLESLQNFLNGNFISPCVVYDFGLLETVLDEFKNQ
                     IPVTFNYQLFYAVKANSNEKILEFLVDKIDGVDVASLSELDVAKKFFPPTQISVNGPA
                     FSYETLYNLIKKQYKVDINFLEHLQQFSPKESVGIRVTEPDELNNRMSRFGINICSDN
                     WTSNLQNPLITRLHFHFGEKDDKFIVKLDKILFKLQEINKLREVREINLGGGFMKLFM
                     ENRLKEFFLSLMEIYKKYDIDSTVTTIIEPGSAITSFSAYMITSPVNVSEVNEQQVIT
                     LDTSIYTNTLWFVPHIITTLNSSSKERYSTILYGNTCYEHDKYKMKVSLPRLTQNSSI
                     VFFPVGAYIKSNHSNLHRNDFMREVYLWTKNLTY"
     gene            4738..6129
                     /locus_tag="VQU28_001238"
     CDS             4738..6129
                     /locus_tag="VQU28_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476595.1"
                     /translation="MDKKLDILDKVKEYLGNKTTQILDNQYKEFLKLNDIRRAFGISE
                     KVLNNSFNFTSKEFNDLINNENYLFEYACRIREEWRKKCFNHSYRFLCSPIITDDFLN
                     TKTLRSSQIEYKYERYLSKSSIGDRAVDGFVSFNTLTANGMSAIKLCLEILNSIFFKK
                     KIDLLYSTGYYETRFLLNNLAKSGISCYEVSNCELDKDKFYNVFMMEPNRADLTLQKT
                     DFKIVEYFVKYKNNSIKVVILDISYQGSNFKLVEFLEKFKFANVIIFVVRSLIKLDQM
                     GLELTNGGIIEVFIPNHLRKLKNFIEEEFNKFRNSHGANLSLYEYCLLDNSLTLKNDW
                     NYSDLVMKFTSNFYADIKDLFMENSDIEIIHEEGVPFVFLDLIGEGKKEYEMFFQWLN
                     FFYKQLGITLYARNSFGFRNLTVEYFGIIGTERYIFKICPGVYKGLSYYLMKFLLKSF
                     SNEYLKTTDEVNR"
     gene            6126..7304
                     /locus_tag="VQU28_001239"
     CDS             6126..7304
                     /locus_tag="VQU28_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2476596.1"
                     /translation="MKNLIKLLIIRLIVNLADSVFYIVALWHVSNNYSSSMFLGIFIA
                     VNYLPDLLLIFFGPVIDRVNPQKILIISILVQLAVAVIFLLLLNQISFWVIMSLVFIS
                     VMASSISYVIEDVLIPQVVEYDKIVFANSLFSISYKVLDSIFNSFASFLQVAVGFILL
                     VKIDIGIFLLALFILLLLKFRTSNANIENFSFKYYKREVLQGTKFILNNKLLFKTSIS
                     LTLINFFYSFQTVVVPIFSIRYFDGPIFYGIFLTIAGLGGILGNMLAPIVIKYLKSNQ
                     IVGVFLFLNGSSWLVAIVIKDYTLSLILFFVCFMSKGVFNIIFNSLYQQIPPHQLLGR
                     VNTTIDSIISFGMPIGSLVAGTLIDLNIELVLIAISIPYFLFSYIFYTDNGLKEFSIY
                     "
     gene            7729..7962
                     /locus_tag="VQU28_001240"
     CDS             7729..7962
                     /locus_tag="VQU28_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000623387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2476597.1"
                     /translation="MISIDLGGTNIKITVLSNDGEIETLWSITTDTSEKGSQIISDII
                     SSIKNKLTERNIPDSDLLGIGMGSCSSYFPCKS"
     gene            8245..10194
                     /gene="mutL"
                     /locus_tag="VQU28_001241"
     CDS             8245..10194
                     /gene="mutL"
                     /locus_tag="VQU28_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HET2476598.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKIQITDNGYGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(10519..10986)
                     /locus_tag="VQU28_001242"
     CDS             complement(10519..10986)
                     /locus_tag="VQU28_001242"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HET2476599.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(10987..12192)
                     /locus_tag="VQU28_001243"
     CDS             complement(10987..12192)
                     /locus_tag="VQU28_001243"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HET2476600.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(12212..12847)
                     /gene="ribE"
                     /locus_tag="VQU28_001244"
     CDS             complement(12212..12847)
                     /gene="ribE"
                     /locus_tag="VQU28_001244"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HET2476601.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(12832..13932)
                     /gene="ribD"
                     /locus_tag="VQU28_001245"
     CDS             complement(12832..13932)
                     /gene="ribD"
                     /locus_tag="VQU28_001245"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HET2476602.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHHVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(14067..14181)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            14338..14931
                     /gene="ruvA"
                     /locus_tag="VQU28_001246"
     CDS             14338..14931
                     /gene="ruvA"
                     /locus_tag="VQU28_001246"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HET2476603.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            14941..15504
                     /locus_tag="VQU28_001247"
     CDS             14941..15504
                     /locus_tag="VQU28_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HET2476604.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRDFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            15501..16178
                     /locus_tag="VQU28_001248"
     CDS             15501..16178
                     /locus_tag="VQU28_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2476605.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     VGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(16319..17350)
                     /locus_tag="VQU28_001249"
     CDS             complement(16319..17350)
                     /locus_tag="VQU28_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HET2476606.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRALFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNAGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(17605..18288)
                     /locus_tag="VQU28_001250"
     CDS             complement(17605..18288)
                     /locus_tag="VQU28_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HET2476607.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASVFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(18300..19244)
                     /locus_tag="VQU28_001251"
     CDS             complement(18300..19244)
                     /locus_tag="VQU28_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HET2476608.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            19376..22207
                     /gene="uvrA"
                     /locus_tag="VQU28_001252"
     CDS             19376..22207
                     /gene="uvrA"
                     /locus_tag="VQU28_001252"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HET2476609.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAIKASSVEQIVDKVLELPERQRLQILSPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDNSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPNASKTLHEGALAPWNPISSNYYPNMLEQAMTAFGVAMDKP
                     FEDLSEEDKNLILYGSDGKKFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCHGYRLNDQALSVRVGGEQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            22200..23261
                     /locus_tag="VQU28_001253"
     CDS             22200..23261
                     /locus_tag="VQU28_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2476610.1"
                     /translation="MNKRVQAFLAKMQEKELDGVIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
BASE COUNT         7035 a         4105 c         4754 g         7418 t
ORIGIN      
        1 agatctgaaa taactgttat gatatagagg attgaaacta gaatagtaca cctctgcttc
       61 taaaacattg ttagaaatcg atttgactgt cctgatcgat ttatcctgtt cttatttcat
      121 ttcactatag taaggtgcag caggataaag aaaaaccgag aagtttcttt ctcggatttg
      181 cttattatga ggttcctatt ttctatgctc caagagttga ttgatgtgat ctactttttt
      241 agcctctctt ttatagagaa tagcccagat gatgagatag acaatcgcaa actcgataat
      301 gagctggagg tagaaaatcc agtggaaagg gaaccaacca gcaagagttg ctagtgggac
      361 aaagccagcc agcataagga agaaatgagt cagagtggca cgaagcatgc tccagtcacg
      421 gctgaataag cgattgccaa agttgaagag cataccaatg gctgcccaga taagtgtgca
      481 gtagagcaag accaaggcac cgtgaacctg atgctgatcc atcacttggc cgatgagaga
      541 gtagggattt agtggtgcgt aggtatttgg tgcataaatg agtgaaaaga ggatagagag
      601 gatgaggccg ataagaacac cggcagctgc atcgtgaaat acttgttttt tcatagttct
      661 aatttctcct tgatggtttt tagataacgg cgtgaagagt aggtgaagct ttcgtttttc
      721 aagaaaattt ctaccagacc gtttggcgtg agcttgaggt gagagatgga atcgatattg
      781 atgatttctg attgggaaat ttggataaaa ttggttggca agagtttaag aacctgatag
      841 agtcgcaaat caatgctgta ggtctgactc gcggtttctg ctagaacctt ccgattctcg
      901 atatagaagc gctgaatctt gccaatctca actagataga cctgatcatc gattttccct
      961 ttgatttttt ctctttggtc cagattttct gcgaactcga tgactttctg aactttatct
     1021 gtcggctgag gtgcttggac aatcagcttt tcctcctcgt aagtctcact aatctgtaat
     1081 tctactttca taaatttctc tccttgtttt tcatacaaaa ttaatctcac ttcttgtaca
     1141 cctttattaa tactcttcaa aaatctcttc aaaccacgtc agcgtcgtct tgccgtagga
     1201 tagttgttga ctttgtcagt tttatctaca acctcaaaac agtgttttga gctgtctgcg
     1261 cctagctttc tagtttgctc tttgattttt attgagtata ccactatttt actccctctg
     1321 gcaaggtact ttgtctatgt ggagggattg ggctcctatg tggtggagct tttctgttct
     1381 ttctgaaata tggtataata gcactaatca atttctagga aaatagatac agaaaggggc
     1441 tgaaagaact aggggaacag ttgtaattag aaggtgatag gtatgaaaag tggcgttgaa
     1501 gctggataag tttataaaga gtttgaatga agaaatgaaa agtaaataag ttgatgatga
     1561 attgcaaaaa tagattttat atacaatttg tatctttcaa atcattgatt tgaattttag
     1621 atttaatcat gttacaatta aaaatgaaat caaatagaag aggaagatgc aaacgtgggc
     1681 acactattag caacaagatt aaaaaataga cgaaaagaat taaaaatgtc tcagcgagaa
     1741 ctagctgaag gtatctgtaa acaggggcaa atcagtcgat tagagagcgg agagtttact
     1801 cctggagcag atttcttgca tgcccttgct aagaagttaa aagtcagtat ggattatttt
     1861 tttgatgagc aggttgtaga gaaagttgag gagttatcag aatttaagaa attagcacag
     1921 acatttatta caaatcgcaa ttatgagtct ttgaaatata tatatgaatt ggagaatgta
     1981 aaagctcacc gcctatcttt agcagataaa atctacatgg aatggataaa atctctgata
     2041 gatttttatt tttacggaca gaaagaagat gcgattgtac gattagaggg ggtattatca
     2101 cagttaagtg tatctgattt gaactatctc caagtttcaa acaccctatt caatttttat
     2161 tatgatattg gagatttagg tcgtttcaat aaaattcgtg aaaaactaga gtatcaagta
     2221 aatcacctca atttgagtac aatcgaagag ttagaactat ctattaagtt taactacaac
     2281 gtttgtcgtt atctatggtt acagaaaaat attgaagaag ctattactaa aattacagct
     2341 actataaaac aatgtaaggc ttacagaacg aattatcttt tggctgattt gtacttattg
     2401 atgggaaatg taagtgaaaa tttttcttct aaaagttcgg tgaaagaata ttttgagacg
     2461 gcatattttc tatacaaact tgaggagaac atgtcaatga ctctgaaagt tgagcattac
     2521 attgcagata taacagaata atgaatacgg tgttcaagtg agcatcgttt ttcaaaccta
     2581 gttcagatac aaaaaagtat caaataaaaa aaaataaaaa aacattataa aaacaattga
     2641 caagctaatt ttgaagtgat aaaataaatg caagaaaaca aaaggagagg tatatgagaa
     2701 gtttgtttaa gaaaatagtt gctgtattag ttattggctt aattctgtta ggaattgcga
     2761 gaacacctca agttcataag atggctagag ggattgatcc tggtcctgcg aattttattt
     2821 agaaatcaat ttctactaat tgagtactaa ttgagtatta atgggtaaaa agattgtatg
     2881 gataagaaaa atttacttgt attggttact ggttcggtag gtgccagtaa tgtagataca
     2941 tacttatatt atataaaaaa attctataat gttaaagtga tactttctga aaattctaaa
     3001 aaatttataa gtaaggaatt aattagttat ttttgtgata aagtttatga tgaaatattt
     3061 gttgaagagg tagttccgca cgtttttctg ccatatgaat ctgacttact tcttatttta
     3121 ccagctacgg caaatgtgat tggcaaaatt gctaatggta ttgctgatga tttagttaca
     3181 gcaactgttt taaactttaa taaaaaaata attttttgtc ccaatatgaa ctctactatg
     3241 tgggacaatc acatagttca aagaaatgta tcaattctaa aggagttggg acatatattt
     3301 ttatttgagt ctaaaaaaac atatgaggta ggattgcgta aagcaataga ttcaacatgt
     3361 tcaatgttac aaccacagtc gttagtaaaa gaacttatca aattagaaaa tattgtcctt
     3421 gaagagggac attaaaaact actgagaata ttaaggaggg gaaaaaatgg aaaattcatc
     3481 aatcgatgta gatatgctgt tggaagaatt gacacaagaa gcaatggtcg ttgttgctgt
     3541 tgataaggac tgttaattta aacttatggc aatatatgaa aggttactgg atgttttaaa
     3601 ttatgcaggc agtagccttt tattatatac aaatggataa agtaaggata atacaatgat
     3661 taataaaaaa atacaacaag ttgttttgga atcattacag aattttttga atgggaactt
     3721 catttcgcct tgtgtagtct atgattttgg cttgctggaa actgtacttg atgaatttaa
     3781 aaatcaaatt cctgtaacat tcaattacca acttttttat gccgttaaag caaattcaaa
     3841 tgagaagata cttgaattct tagtagataa aattgatgga gttgatgtgg cgtcattatc
     3901 tgaattagat gtggctaaaa aatttttccc accaactcaa atttctgtta atggtcccgc
     3961 attttcttat gaaactttat ataatctgat taaaaaacaa tataaagttg atattaactt
     4021 tttggaacat cttcaacaat tttccccaaa agaatctgtt ggaataagag taacggagcc
     4081 agatgaactt aataatcgta tgagtcgatt tggaataaat atttgcagtg ataattggac
     4141 tagtaattta caaaatcctt taattacacg actgcatttt cattttggag aaaaagatga
     4201 taaatttatt gttaagttag ataaaatatt atttaagtta caagaaatta ataaacttag
     4261 agaggttaga gaaataaatc ttggaggcgg ttttatgaaa ttatttatgg aaaatcgttt
     4321 gaaagaattt tttctatcac ttatggaaat ctataaaaag tacgatattg atagtactgt
     4381 gactacaata atagaaccag gtagtgcaat tacttcattt tctgcctata tgattactag
     4441 cccagttaat gttagtgagg tgaatgagca gcaggttatc acgttagaca catcaatata
     4501 caccaataca ttatggtttg ttccgcatat tattacaacg ttaaattcaa gtagtaaaga
     4561 gcgttatagt actattctct atggtaatac ctgttatgaa catgacaagt ataaaatgaa
     4621 agtttcgctt ccaaggttaa ctcaaaatag cagtatagtg ttttttcctg taggagctta
     4681 tataaaaagc aatcattcaa atttacatcg taatgatttt atgcgggagg tatatttgtg
     4741 gacaaaaaac ttgacatatt agataaagtt aaggaatatt taggaaataa aactactcaa
     4801 attctggata atcaatataa agaatttttg aaacttaatg atataaggcg agcgtttggt
     4861 atttcagaaa aagtattaaa caattctttt aattttacga gtaaagaatt taatgattta
     4921 attaataacg aaaattattt attcgaatat gcatgtagaa ttagagagga atggagaaaa
     4981 aaatgcttta atcattctta tcgttttcta tgctcaccta taattacaga tgattttctt
     5041 aacacgaaga cattgagaag tagccaaatt gaatataaat atgagcgata tttatcgaaa
     5101 agttcgatag gcgatagagc ggttgatggc tttgtttcct tcaatacttt aacagctaat
     5161 ggtatgtctg ctattaaact atgtcttgag atattaaact ctattttctt caagaagaag
     5221 attgatttat tatattcaac cggatattat gaaacaagat ttttattaaa taatcttgct
     5281 aaatcaggta ttagttgcta tgaggtaagt aattgtgaat tggataaaga taaattttat
     5341 aatgtattca tgatggaacc caatcgagcc gatttaacat tacaaaaaac tgatttcaag
     5401 atagtagaat attttgttaa gtataaaaat aattcaataa aagtcgttat tttagatatt
     5461 tcatatcaag gttctaattt taaattagta gaatttttag agaaatttaa atttgcgaat
     5521 gtaattattt ttgtggtacg atctttgata aaattagatc aaatgggatt agaattgaca
     5581 aatgggggaa taatagaagt gtttattcct aatcatttga gaaagttgaa aaattttatt
     5641 gaagaggaat tcaataaatt tagaaattct cacggagcta atctaagcct ctatgaatac
     5701 tgtttgcttg ataattcttt aaccttaaaa aatgattgga actattctga tttagttatg
     5761 aaatttacga gtaattttta tgctgatata aaagacttgt tcatggaaaa ttctgatatt
     5821 gaaatcatcc atgaagaggg agtacctttt gtatttttag atttaatagg tgaaggtaaa
     5881 aaagaatatg aaatgttttt tcaatggtta aacttctttt acaaacagct tggaatcaca
     5941 ttgtatgcta gaaatagttt tgggtttcgg aatctaacag tagagtattt tggaattatt
     6001 gggacagaaa gatatatatt taagatttgt ccaggtgttt ataaagggtt aagttattat
     6061 ttgatgaaat ttttattaaa atctttttca aatgaatatt taaaaactac tgatgaggtt
     6121 aatagatgaa aaatttgata aagttgctaa taattagatt gattgttaac ttagcagaca
     6181 gtgtatttta tatagtagca ttgtggcacg ttagcaataa ttattcttcg agcatgttct
     6241 taggaatatt tattgcagta aattatctac cggatttgtt actaatcttt tttggaccag
     6301 ttattgacag agtaaatccg caaaaaattc ttataatatc aattttggtt caattagcag
     6361 tggctgtaat atttttatta ttattaaacc aaatatcatt ttgggtgata atgagtctag
     6421 tgtttatttc agtaatggct agctccataa gttacgtgat agaagatgtg ttgattcctc
     6481 aagtggtaga atatgataag attgtatttg caaattctct ttttagtatt tcgtataaag
     6541 tattagattc tatttttaat tcattcgcat catttttaca ggtggcagta ggatttattt
     6601 tattggttaa gatagatata ggcatatttt tacttgctct atttatattg ttgttgttaa
     6661 aatttagaac tagcaatgcg aatatagaaa acttctcttt caaatattac aagagagaag
     6721 tgttgcaagg tacaaagttt attttaaata ataaattatt atttaaaacc agtatttctt
     6781 taacgcttat aaactttttt tattcatttc agacagtagt tgtaccgatt ttttctattc
     6841 gatattttga tggtccgatt ttttatggta tttttttaac tattgctggt ttgggtggta
     6901 tattgggaaa tatgctagcg ccaatcgtaa taaaatattt aaaatcgaat caaattgttg
     6961 gtgtatttct ttttttgaac ggctcaagtt ggttagtagc aattgttata aaagactata
     7021 ctttatcact tattttattt ttcgtttgtt ttatgtctaa aggagtcttc aatattattt
     7081 ttaattcgtt gtaccaacaa atacctccac atcaacttct tggtagggta aatactacca
     7141 ttgattctat tatttctttt ggaatgccaa ttggtagttt agttgcagga acgcttattg
     7201 atttgaatat tgaattagtg ttaattgcta ttagcatacc ttattttttg ttttcttata
     7261 ttttttatac ggataatgga ttgaaagaat ttagtatata ttagaaatgt ttatgttcat
     7321 tcaaaagcat aatgactata actgaaaaag aaaagtgata tctttaaggt tgttcttctt
     7381 ggtggtgaga ttcgtgagac aacccaagct tttgtcggaa agattaccaa tgctttgatg
     7441 gataggatgt actttagcaa gatgttttta gtggtaacgg tatcgtggat ggacgtgtaa
     7501 taacctcttc tttcgaggag tattttacta aaaaactagc cttggagcgt tccccagaaa
     7561 cggacttact cattgactct tcaaagattt ggggagaaga ttttgcttca tctgttcctt
     7621 gaaaaaagtc acagcagtca tcacagacga tagtactgaa caaaactatg aagagttaga
     7681 aatttatacg caggtgattg tataaaggat ctggaaatag ataagaagtt gattagtatt
     7741 gacctaggtg gtacaaatat taagattact gttctttcaa atgacggtga gattgaaact
     7801 ttgtggagta ttacaacaga tacaagtgag aaaggttctc aaattatatc ggacatcatc
     7861 agttctatta aaaataaatt gaccgaacgg aatattcctg atagcgacct tcttggaatc
     7921 ggtatgggaa gttgctcatc atactttcct tgtaaatcat aggggctata aactctccgt
     7981 ctacttgtcc tgcaacaatt gaagtctgct caaaacgccg tccgctaatc ttttcataga
     8041 ctttctccct tttaggagcc tagctttcta gtttgttctt tgatttttat tgagtatacc
     8101 actattttac tccctctggc aagggacttt gtctatgtgg agggattggg ctcctatgtg
     8161 gtggagcttt tctgttcttt ctgaaatatg gtataatagc actaatcaat ttctaggaaa
     8221 atagatacgg aaaggggctg aaagatgtct catattattg aattgccaga gatgctggca
     8281 aaccaaatcg cagctggaga ggtcattgaa cgtcctgcca gcgtggtcaa agaattggta
     8341 gaaaatgcca ttgacgcggg ctctagtcag attatcattg agattgagga agctggtctc
     8401 aagaaaatcc aaatcacaga taacggttat ggaattgccc acgatgaggt ggagttggcc
     8461 ctgcgtcgcc atgcgaccag taagataaaa aatcaagcag atctctttcg gattcggacg
     8521 cttggttttc gtggtgaagc cttgccttct attgcgtctg ttagtgtctt gactctgtta
     8581 acggcggtgg atggtgctag tcatggaacc aagttagtcg cgcgtggggg tgaagttgag
     8641 gaagtcatcc cagcgactag tcctgtggga accaaggttt gtgtggagga tctctttttc
     8701 aacacgcctg cccgtctcaa gtatatgaag agccagcaag cggagttgtc tcatatcatt
     8761 gatattgtca accgtctggg cttggcccat cctgagattt cttttagctt gattagtgat
     8821 ggcaaggaaa tgacgcggac agcagggact ggtcaattgc gccaagcaat cgcagggatt
     8881 tacggtttgg tcagtgccaa gaagatgatt gaaattgaga actctgacct agatttcgaa
     8941 atttcaggtt ttgtgtcctt gcctgagttg actcgggcta accgcaatta tatcagcctc
     9001 ttcatcaatg gtcgttatat caagaacttc ctgctcaatc gtgctatttt ggatggtttc
     9061 ggaagcaagc ttatggttgg acgttttcca ctggctgtca ttcacatcca tatcgaccct
     9121 tatctagcgg atgtcaatgt gcatccaact aagcaagagg tgcggatttc caaggaaaaa
     9181 gaactgatga ccctggtttc agaagctatt gcaaatagtc tcaaggaaca aaccttgatt
     9241 ccagatgcct tggaaaatct tgccaaatcg actgtgcgca atcgtgagaa ggtggagcaa
     9301 actattctcc cactcaaaga aaatacgctc tactatgaga aaactgagcc gtcaagacct
     9361 agtcaaactg aagtagctga ttatcaggta gaattgactg atgaagggca ggatttgacc
     9421 ctgtttgcca aggaaacctt ggaccgattg accaagccag caaaactgca ttttgcagag
     9481 agaaagcctg ctaactacga ccagctagac catccagagt tagatcttgc tagcatcgat
     9541 aaggcttatg acaaactgga gcgagaagaa gcatccagct tcccagagtt ggagtttttc
     9601 ggacaaatgc acgggactta tctctttgcc caagggcgag atggacttta catcatagat
     9661 cagcacgctg ctcaggaacg ggtcaagtac gaggagtacc gtgaaagcat tggcaatgtt
     9721 gaccagagcc agcagcaact cctagtgccc tatatctttg aatttcctgc ggatgatgcc
     9781 ctgcgtctca aggaaagaat gcctctctta gaggaagtgg gcgtctttct agcagagtac
     9841 ggagaaaatc aatttattct acgtgaacat cctatttgga tggcagaaga agagattgaa
     9901 tcaggcatct atgagatgtg cgacatgctc cttttgacca aggaagtttc tatcaagaaa
     9961 taccgagcag agctggctat catgatgtct tgcaagcgat ctatcaaggc caatcatcgt
    10021 attgatgatc attcagctag acaactcctc tatcagcttt ctcaatgtga caatccctat
    10081 aactgtcctc acggacgtcc tgttttggtg cattttacca agtcggatat ggaaaagatg
    10141 ttccgacgta ttcaggaaaa tcacaccagt ctccgtgagt tggggaaata ttaaaagtat
    10201 aaaaaagtct gggaaaaatt ttcaaaatca gaaaaacgca taaaatcagg tgttcaaaaa
    10261 ccttgatttt atgcgtttta tcatggaaat agttacttca tgttttccta attcttttcg
    10321 aaactctttt taaaccacgt cagttttatc agtaatctca aaacagtgtt ttgagctaat
    10381 tttgccagtt ttatctgtaa catcgaagtt gtgtttgacc actctgcgac tggtttccta
    10441 gtttgctcta tgattttcac agagtattaa attgcgattt tgccaagttt ctttattcgt
    10501 ctaaaagtag agtctgttct atgcgtctaa tgtacgaatt aggttgacca tttcaatagc
    10561 tccttgtgca cactcagaac ccttatttcc tgctttggta ccagctcgtt ctatggcttg
    10621 ttcaattgta tctgtcgtta gcacaccaaa cataacagga atttcgctat ttaaactgat
    10681 ttgggcgatt cccttagata cctcgctaca tacataatca taatgacttg tattccctct
    10741 aatgacagct cccaagcaga taattgcatc atatttttta ctttttgcca tttttgatgc
    10801 aatcagtggt atttcaaaag ctcctggaac ccaggctacc tctatatctt tctcgtttac
    10861 attctctctt ttgagattat ctagtgctcc agataataat tttgaagtta taaattcatt
    10921 aaatctcgct acaacaatac ctattttaat attgtttgct actaaattac cttcataagt
    10981 gttcatttat ttttcctcca tatttaaaat gtgacccatt cgatttttct ttgtttctaa
    11041 ataaaaacta tcgtaaggat tggcttctat ttcgattgat attctactgg aaatggtaat
    11101 tccatatttt tctaactgtt caaccttgtc aggattattt gtcagtaaat gaagtgactg
    11161 aagtcccaga tctttaagca tttgtgctcc aatatgatat tctcttaaat caccttcaaa
    11221 gcctaatgca agattggcat caagcgtatc catgccttga tcttgtaaat gataggcttt
    11281 taatttattg ataagtccaa ttcctcgtcc ctcctgtcgc aagtaaagta agacacccga
    11341 accattctca acaatcattt tcatagcttt atcgaattgc tgtccacaat cgcaacgtaa
    11401 agagcctaaa acatctcctg ttaaacattc ggagtggacc cgacataata cattggcttc
    11461 atcctctata tttcccataa taagagcaag atgatgttcc ccatttagtt tatctatata
    11521 gctaattgct ttgaaattac cgtatctagt aggcatattg acagttgaaa ctcgttctac
    11581 cagctgatca tatacttttc tatattcttg taattctttg atggtaatta gtggaatgtt
    11641 gtgtttcttc gagaactgaa ttaaatcatc tgttctcatc attttgccat catgattcat
    11701 tatttcacaa cataggccac actcttttag tccagctaat tttaataaat caacagttgc
    11761 ttctgtgtgt ccatttcttt ctagaacacc accttttttt gcaattaaag gaaacatgtg
    11821 tcctggcctg cgaaaatcag agggtgttat atcttcagct acacacatac gtgcggtcag
    11881 tcctctctcc tcggcagaaa tacctgtggt cgtttcttta taatcaattg aaactgtaaa
    11941 agcagtctta tgattatctg tattgttttc aaccataggt gaaagcatta attgattagc
    12001 taaactttcg ctcataggca tacaaattaa tcctttggca taagtagcca taaaattaac
    12061 attttctgtt gtagctgctt gtgcagaaca aattaagtct ccttcatttt ctctatcctt
    12121 gtcgtctata acaagaacaa gtcgtccctt ctgcaatgct tctaatgctt cttgtatttt
    12181 tcgatattcc attgactgat tatcctttct gctaaaatcc attttgatat aatagttcct
    12241 tagatatttc tgattttgga gagttatcca tcagtttttg cacatattta cctaagatat
    12301 cattttcaag atttactgta ctcccgactt gtttactctt aagaatggtt tgttccaagg
    12361 tatgagggat aacagatact gaaaagttta ctttggagac tttagcgaca gtcagactaa
    12421 tgccgtcaat tgtaatagat cctttttcaa ctattaaatc taaaatttct ttttgtgtgt
    12481 tgatttgata ccatacagca ttatcatctt tttttattga cgagattttt cctgtaccat
    12541 caatgtgtcc tgtaacgacg tgacccccaa gtcgaccgtt gacagataag gctctttcta
    12601 gattcacctc acttccatgt tttaatagag taagagctgt tcgactccat gtttcattca
    12661 ttacatcaac tgtaaaggat tgatgattga aatgagtaac tgtaagacag ataccattta
    12721 ctgctatact atcgcctaaa tggatatccg ttaatatttt tgaggcttta attgatagtt
    12781 tacaattacg agagtctttc tgtattcttt caacttttcc gatttcttca attattcctg
    12841 tgaacatgga taaatcactt cactttctat gagatagtca tttcctattt gagaaaaagc
    12901 ataaggtttc aatctaatag cgtcatttgg caaagaaatg ccttcacctc cgacaggaaa
    12961 cttggcactg cctccaaaaa tttttggtgc aatatatatt ttcagctcat caacaatttg
    13021 ttgttccaaa gcactccaat tcattagact gcccccttct agaactaggc tatcaatctg
    13081 catgtttcct agatgttgca ttaaactcga taagtctata tgattgcctt ttttctttat
    13141 ggaaagtatt tcacagccat gattttgata tagcttcatt ttatttttgt cttcagagga
    13201 agtggcaatg taagttttaa tatcatttgc tgtttttacg attttagagg taagaggagt
    13261 tcgtaaatgt gtatcgcata tgatacggat aggatttttc ccttcctcca atctacatgt
    13321 cagcaaagga tcgtcttgaa taacagtatt gactcccacc ataattgcac taacatggtg
    13381 tcgtaactga tgcacatgct ttcttgcttc ttcttcagta atccatttgg attgatttgt
    13441 tttagtggct atttttccat ccattgacat tgcatatttc ataaaaacat agggtacatg
    13501 ctgggtaata tactttctaa aactttttat taagttaaga cactcatttt ctaaaattcc
    13561 aacagtaact tgaagattat tttcctcaag tatctttact ccttttccag atacaatagg
    13621 attacagtct aggcttccaa tgactactct tgtaatacca ctatcgatta tagcatctat
    13681 acagggaggt gttttcccga agtgacaaca gggttcaagt gttacataaa gcgtcgctcc
    13741 gacaggggat tctctacagt ttttaagagc atttctctca gcatgtgggc caccaaaaaa
    13801 ctcatgataa ccttgtccga tgatgtgatt atcttttaca ataactgcgc cgaccatagg
    13861 attgggattg acgtaaccag cccctttttg tgccagtttt attgctaatt tcatatattt
    13921 tgaatcgctc atctcactac ctccaaaaaa aatatacctt gaatagggga ctactcaagg
    13981 catacaaaag aaaacttatg cgattaacaa aaatactctg aaatgacaag taatcatttc
    14041 agagcacgca aaaagcacaa atatactttt atcttctttc atccagacta tactgtcggc
    14101 tttggaattt caccaaatca tgcctttcgg ctcgtgggct ataccaccgg tagggaatta
    14161 caccctgccc tgaagatagt tattcaatta cagatgatta tagtacttaa ttttgaatat
    14221 gtcaacagat aaataccgat tgtttttgat atactgtatt tgtgataatc gattctcgct
    14281 cctcggataa agaaaatatg atatactaga taaacgaaat aagagagaag gaatactatg
    14341 tacgcatatt taaaaggaat cattaccaaa attactgcca aatacattgt tcttgaaacc
    14401 aatggtattg gttatatcct gcatgtggcc aatccttatg cctattcagg tcaggttaat
    14461 caggaggctc agatttatgt gcatcaggtt gtgcgtgagg acgcccattt gctttatgga
    14521 tttcgctcag aggatgagaa aaagctcttt cttagtctga tttcggtctc tgggattggt
    14581 cctgtatcag ctcttgctat tatcgctgct gatgacaatg ctggcttggt tcaagccatt
    14641 gaaaccaaga acatcaccta cttgaccaag ttccctaaaa ttggcaagaa aacagcccag
    14701 cagatggtgc tggacttgga aggcaaggta gtagttgcag gagatggcct tcctgccaag
    14761 gtcgcagtgc aagcaagtgc tgaaaaccaa gaattggaag aagctatgga agccatgttg
    14821 gctctgggct acaaggcaac agagctcaag aaaatcaaga aattctttga aggaacgaca
    14881 gatacagctg agaactatat caagtcggcc cttaaaatgt tggtcaaata ggagcagaga
    14941 atgacaaaac gttgttcgtg ggtcaagatg accaacccac tctacatcgc ctatcatgat
    15001 gaggagtggg gccagcccct ccatgatgac caagcgttgt ttgagttatt gtgtatggaa
    15061 acctatcagg caggcctgtc ttgggaaacg gtactcaaca aacgccaagc tttccgagaa
    15121 gcctttcata gctatcaaat tcactcagtc gcagagatga ctgacactga attggaagcc
    15181 atgctggaga atccagctat cattcgaaat agagccaaga tttttgctac acgcgctaac
    15241 gcccaagctt ttctacagtt acaggcagag tacggctctt ttgatgccta tctttggtct
    15301 tttgttgagg ggaaaactgt cgttaacgat gttcctgatt atcgccaagc cccagctaaa
    15361 acacccttat ctgagaaatt agccaaagat ctcaaaaaac gagacttcaa gttcacaggc
    15421 ccagtcgccg tattgtcttt tctacaggct gcagggctag ttgatgacca cgagaatgat
    15481 tgtgagtgga aaggtcttaa atgatgtcta acaaaaataa gggaattctg atttttgcga
    15541 ttctctatac agtcctcttt gtgtttgatg gcgttaaatt gctggcttct ttaatgccat
    15601 ctgccattgc aaattatctt gtttatgtag ttttagctct atatggctcc ttcttattca
    15661 aggatagatt gatccaacaa tggaaggaga ttagaaagac taaaagaaaa ttcttctttg
    15721 gcgtcttaac aggatggctc tttctcattc tgatgactgt tgtctttgaa tttgtatcag
    15781 agatgttgaa gcagtttgtg gggctagtcg gacaaggtct aaatcagtct aatattcaaa
    15841 gcacttttca agagcaacca ctactgatag ctgtttttgc ttgtgtcatt ggacctctgg
    15901 tagaagaatt atttttccgt caggtcttat tgcattactt gcaggaacgg ttgccaggtt
    15961 tactaagcat tattctggta ggacttgttt ttgctctgac tcatatgcac agtttggctc
    16021 tatcagagtg gattggtgca gttggttact taggtggagg ccttgccttt tctattattt
    16081 atgtgaaaga aaaagagaat atctactatc cactacttgt tcacatgtta agcaacagcc
    16141 tctccttaat cattttagct atcagtatag taaaatgaaa taagaacagg acagtcaaat
    16201 cgatttctaa caatgtttta gaagtagatg tgtactattc tagtttcaat ttgctgtaat
    16261 atgtgatgaa aatgccagta atgataccga gaaaaaagct gagaaacttt tcccagcttt
    16321 atttgttata gtcaaagcga atgacttgtt cctgtgcatc tacatgagca tggaccccaa
    16381 agggtacaat tgctcttgga gttgcgtggc cggcattcag attatagaca atcgggatat
    16441 tgctgtcaat gatatccaat agtgcctctt tatagtcgtc atggaaagtt tcatccatag
    16501 gttttccgac caagagtcca ttgatgaccg cgaatatgcc agtgtccttt aaagttagca
    16561 acatcttttt gaagtcttct ggcttaggct tttcttcgct tgtttcgagc aagaggattt
    16621 tcccttccca gtctgacaag tcagggaaaa gtttgtattt ttggcagagc tccgtgctat
    16681 ctgcgtatcg agagttgtca aagatatcgt agagggattc gaggcaacca ccgaggattt
    16741 tcccctcgaa tagtgccctt ccttgcaaca agtcaaaacc tgtatttgta tgactgacac
    16801 gaggtgttcc cagggccgtg ggactaaaat cagttcgttc ctcataccaa acgtcactag
    16861 ggcggatttc tgaaattctt cccgtctcaa tcaattcttt aaagtagtga aggctatagg
    16921 ctagcatttc tttgtctaat tcacaaatgt ctgctaaaaa ggattgacca taaaaagtct
    16981 tgattcctaa tttatgcaac atgaggtggt tcatggttgt atccgagaag ccaagaaaaa
    17041 ttttttgctt gataaccttt tggagttggt cattttcaaa aagataaggt agcaagcgat
    17101 aggtatcgtc tccaccgatg gcacatagga tcatgtcgat gctatcatca gaaaaggcat
    17161 gaatcaaatc ctctgcacga gcttcaggat ggtccttgat aaagtctaat ccttttaacg
    17221 aatggggcaa aaagatggga ttgagtccca gatccttgag acgttggata cccaagtcca
    17281 cttcgtgttt gacaaaatcc tctccgataa tgccactaga taaactaaca ataccaatag
    17341 tagaaaccat atctcatcct cctagaaata gattgagcct attttatcac aaaatataag
    17401 agaaagctat gtgggatttc agaagaatac ttttaatact cttcgaaaat ctcttcaaac
    17461 cacgtcagtt ttatctgcaa cctcaaagct gtgctttgag caacctgcag ctagcttcct
    17521 agtttgctct ttgattttca ttgagtataa ctcaaaaaag taagtgaaaa agcaaccgca
    17581 cctgcagttg cttttatcgt tttcttattc ttgatagcgt gttggtcctg cacttgcact
    17641 acggatattc aagcgtttct tgagatagaa gcgaagggct aggagggctg ctccaataat
    17701 agctagtggc aatggatcca gtactgggtt aaggctagct ggtaggaagc ttgttgcaaa
    17761 gaagacaagc aaccaaagga acacagaagc taggataact agtacagatt tccagaaagg
    17821 tggacgttga ctgcgatcca tatctggtcc atagtattgg taaacaaagt agtacatcaa
    17881 gtagaaggca aatccaccaa ccagtccaac taatagaaga gtaatcaatc catagccgaa
    17941 agcttgatct gccgcaaaga aggttgtgag ggcgctgaca agggcaaaga ggctagtgat
    18001 gaaaagagct gagtccataa tcatcagttt tgggtcatca ttttcttttg gatgctcttt
    18061 ttcgtactgc tctttgacag tgaagctatg agcccaatga gttggtgcgc cgtataggga
    18121 acgggcagtt gtacctttag attgctcctc aaggatttgg ggaatgactt cctcaaaaat
    18181 agtctggatt tcagcgtctg ttttcccatc tttgatgaat tgttgggtag caatgtggac
    18241 aaactcttgg tttttcttag ttaatttttg tagatcaatt tgagacatag gaactccttt
    18301 tagaaccatt ttttatggat gagatagaga gtgagcgaga cactcatagc aaaggcgata
    18361 aagacgatta accagaaggc atttggctct ccgtttaggg gaatttcatt atccttaaag
    18421 ttcatcccgt aggcagaaaa gaccatggtt gggatggaca tgacgatggt cacaagggcc
    18481 aaggttttca tgatgttgtt ctggttgtta gaaatgatag aggcaaaggt ctctgtcata
    18541 gaatgcaaga cgtttccata aatatctgcc atctcgatgg cctgttgggt ttcaatcagg
    18601 gtgtcttcaa gcaggtcttc gtcctcaagg tatttcttga tattgctggt tgaactggtc
    18661 aatttcttaa tcacgcgctc atttgttttg agggaggcct tgaaatagac gatagttttt
    18721 tccaattcca tgagctcaat caattcttca ttacgagttg attgatgcag ttgactttcg
    18781 atttgttcac tcttgcggtc gattgaacga agggctgtta ggtaaagctc tgcattgcga
    18841 taaagaattt gaaagataaa acgtgaacgc atgaaggtat agaaattacg caatcgacgg
    18901 ttgataaaga catcaaggac aggtagtggt tccaaacacg tagtgataat ggtttcctca
    18961 gtgatgataa taccaagcgg gatggttacg tagtaggtgc ggttatttct ttcctccgtg
    19021 accggcacgt ctacgataat cagggtatac tcgtcttcaa tggtaatacg agacatttct
    19081 tccgcatcga gcggtgctcg aaggtcggca atatcaatat cgaaggtgtt ggcgatttcg
    19141 agtgattcat tttgagtcgg attgacgaga ttgatccaag tacccggttc aagcgtatcg
    19201 atctctttaa attcagttgt tgtagagaga aaaacttgtt tcatatccct tatcctttct
    19261 catttttcag attttttcac actgtactat tatactacaa aatcggcctt ttgggtaata
    19321 gaatctcact ttttttggta taatggtaag caataatgga ctagaaagaa caaagatgca
    19381 agataaaatt gtcattcatg gggcgcgtgc ccataattta aaaaatattg atgtggagat
    19441 tccgcgagac aagttggttg tcgtgactgg cttgtcaggt tcagggaagt ccagtttggc
    19501 ttttgatacc ctctatgcgg agggacaacg tcgctatgta gagagtttgt ccgcctacgc
    19561 tcgtcagttc ttgggaaata tggaaaaacc agatgtagat gccattgatg gtctcagccc
    19621 agctatttct atcgaccaga aaacgactag caaaaaccct cgctcgacgg tgggaacaac
    19681 gactgaaatc aatgactatc tgcgtctcct ctacgcgcgt gtggggacgc cttactgtat
    19741 caacgggcat ggggctatca aggcttcttc tgtggagcaa atcgttgata aggttttgga
    19801 gttacctgaa cgccagcgct tgcagatctt gtctcctgtc atccgcaaga aaaaaggcca
    19861 acataagagt gttatcgaga aggttcagaa agacgggtat gttcgtgtcc gtgtggatgg
    19921 ggaagtctat gatgtgaccg aagtgccaga gttgtctaag agcaagcaac acaatatcga
    19981 tgtcgtggtt gaccgtattg ttatcaagga gggcattcgt agtcgtctct ttgattccat
    20041 tgaggctgcc cttcgtattg cagaaggtta tgtcattatc gacaccatgg acaactctga
    20101 gttgttgttc tctgagcatt atgcctgtcc agtttgtggt tttactgtcc cagagttaga
    20161 gcctcgtctc ttttctttca atgctccttt tggctcttgt agtgagtgtg acggcttggg
    20221 catcaagctg gaggtggata ctgatttggt agtgccaaat gccagcaaaa ccttacatga
    20281 gggagcgctg gcaccttgga atcctatctc atccaactac tatccaaaca tgctagagca
    20341 ggccatgacg gcttttggag tggctatgga taagcctttt gaggacttgt cagaagaaga
    20401 caagaacttg attctctacg gctcagatgg taagaaattc catttccact atgagaatga
    20461 atttggtggc gtgcgcgata tcgacattcc gtttgaggga gttatcaata atatcaaacg
    20521 tcgttaccat gaaaccaata gcgattacac tcgcactcag atgcgcctct acatgaatga
    20581 gctgacttgc ggaacctgtc acggttatcg tctcaatgac caggccttgt ctgtccgtgt
    20641 gggtggtgag caagggccac atatcggaga aatttcagac ctgtctatcg cagatcactt
    20701 ggacttggtg agccagttaa ctttgtctga aaatgaagcc atcattgctc gtcccattct
    20761 caaggaaatc aaggatcgtt tgaccttcct taataatgtg ggtcttaact atctaaccct
    20821 gtcacgttca gcaggaaccc tttcaggtgg ggaaagtcag cgcattcgtt tggcaaccca
    20881 gattggttcc aacctatcag gcgtcctcta tatcctagac gagccgtcaa tcggtcttca
    20941 ccagagagac aatgaccgcc tgattgccag tctgaaaaag atgcgtgact tgggcaatac
    21001 tcttatcgtg gtggaacacg acgaagatac catgcgtgaa gctgattatc tgattgacgt
    21061 tggtcctggt gccggtgttt ttggtggtga gattgttgca gcaggtacac ctaagcaagt
    21121 ggctcgcaac agcaagtcta tcacaggtca gtacttgtca ggcaaacgtg tcatcccagt
    21181 accagaagag cgccgtgtcg gaaatggtcg ttttattgaa gtgataggag cgcgtgagaa
    21241 caacttgcaa aatgtcacgg ctcgctttcc actaggaaaa tttatcgcag tgacaggtgt
    21301 gtcgggttca gggaaatcga ctctaatcaa cagcattctc aaaaaagcca ttgcccagaa
    21361 gctcaaccgt aattcagaca aacctggtaa gtttaagact attacaggga ttgagcatgt
    21421 cgaccgtttg attgatattg accagagccc tatcggacgg acgccgaggt ctaacccagc
    21481 tacctataca ggagtttttg acgatatacg tgaccttttt gctcagacaa atgaagccaa
    21541 gattcgaggc tacaagaagg gacgcttcag tttcaacgtc aagggaggtc gctgtgaagc
    21601 ttgctcaggt gacgggatta tcaagattga gatgcacttc ttgccagatg tttatgtggc
    21661 ttgtgaagtt tgccacggaa cccgctataa cagtgaaacc ctagaagttc actacaagga
    21721 aaagaatatt tcgcaggtct tggatatgac ggtcaacgat gcggtagaat ttttccaaca
    21781 cattccgaaa attcaacgca aactccagac catcaaggat gtagggctag gctatgtgac
    21841 cttgggacag ccagctacca ccctttctgg gggagaagcc caacgtatga agttagctag
    21901 cgaactccac aaacgctcga caggaaaatc tttctacatt ctggatgaac cgacgacagg
    21961 gcttcataca gaggacattg ctcgcctgct caaggtttta gctcgctttg tagacgatgg
    22021 caatacagtc ctcgtcatcg agcacaatct ggatgttatc aagacggcag accatatcat
    22081 tgacttggga cctgagggcg gtgtcggtgg tggaaccatc atcgtaacag gaactccaga
    22141 agaagtagct gccaacgaag ccagctatac aggacactat ttgaaaggaa agttacatca
    22201 tgaataaacg cgtacaagca tttctagcta aaatgcaaga aaaagaacta gatggtgtca
    22261 tcatcaacaa tcttaaaaac gtctattatt tgactggttt ttggggctca aacggaacag
    22321 tctttatcag ccgtgaccgt caggtattag tgacagactc tcgctatatc atcgcagcta
    22381 agcaagaaac cagtggtttt gagattgtgg ctgatcgtga tgaattggct gtcattgcag
    22441 gaattgttaa ggacatgggc ttgactcgta tcggttttga agatgagatt tcagtgtctt
    22501 attaccaccg tatgcaggca gcttttgcag gtttgaactt gcttccacaa actcagtttg
    22561 tggaaggtct tcgtatgatt aaggatgagg cagagattgc agcgattcgc aaggcttgtt
    22621 ctatctcaga ccaagctttc cgcgatgcgc ttgactttat caaaccagga aaaactgaaa
    22681 ttgagattgc caacttcctt gatttccgca tgcgtgagtt gggagcatct ggcttatctt
    22741 ttgatacgat cctagctagc ggtatcaatt cttctaaacc ccatgcccat ccaatgcaca
    22801 aaccagtgga gttgggagaa gccattacca tggacttcgg ctgtctctat gaccactatg
    22861 tcagtgatat gacccggact atctatctag ggcatgttag cgatgagcag gcagagattt
    22921 acaatacggt tctaaaagct aaccaagcct tgattgacca agctaaggca ggcttaggtt
    22981 tccgtgactt tgacaaaatc cctcgtgata ttatcattga ggcaggttat ggtgactact
    23041 ttactcacgg cattggccac ggtattggtc tggatatcca tgaggaaccc tactttagtc
    23101 agacttctac agaaactatt aagacaggta tggccttgac cgatgaacca ggtatctata
    23161 tcgaaggcaa atatggcgtt cgtatcgagg atgatatcct gattacagag acaggttgtg
    23221 aattattgac cctagctcca aaagagttga tagtcattta gttgtttgtc aagaaaaaag
    23281 tacgaaaatg acgaaaaaag tcaaaaaaaa tt
//
LOCUS       DAVBCB010000033        23023 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVBCB010000033 DAVBCB010000000
VERSION     DAVBCB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 23023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23023
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.033"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..337)
                     /locus_tag="VQU28_001254"
     CDS             complement(<1..337)
                     /locus_tag="VQU28_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476611.1"
                     /translation="MDSASIIAHTIHQKFNLKVPNYRQEEDWARMGLPITRKEIANWH
                     IKASQYYLESLYNLLREKLLEQPLLHADETSYRVLESDSQLTYYWTFLSGKSEKQGIT
                     LYHHDQCRSG"
     gene            complement(345..650)
                     /locus_tag="VQU28_001255"
     CDS             complement(345..650)
                     /locus_tag="VQU28_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HET2476612.1"
                     /translation="MFPVETEEITYKRKKSKGKRQALLAQFDSEEVHHQVEESICSDC
                     QGDLKEIGATLQRQELVFIPAQLKRIDHIQHAYKCQACSDKNPSDKIVKAPIPKAPL"
     gene            complement(637..831)
                     /locus_tag="VQU28_001256"
     CDS             complement(637..831)
                     /locus_tag="VQU28_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476613.1"
                     /translation="MKIIQQQSATIDSLTNALALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEKDGDVPS"
     gene            complement(907..1247)
                     /gene="tnpB"
                     /locus_tag="VQU28_001257"
                     /pseudo
     CDS             complement(907..1247)
                     /gene="tnpB"
                     /locus_tag="VQU28_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(1228..1413)
                     /locus_tag="VQU28_001258"
     CDS             complement(1228..1413)
                     /locus_tag="VQU28_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476614.1"
                     /translation="MPQPIVPVEIPQSRPFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MVEQLLDKVLLYDNSSI"
     gene            complement(1717..3279)
                     /gene="guaA"
                     /locus_tag="VQU28_001259"
     CDS             complement(1717..3279)
                     /gene="guaA"
                     /locus_tag="VQU28_001259"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HET2476615.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            3421..4119
                     /locus_tag="VQU28_001260"
     CDS             3421..4119
                     /locus_tag="VQU28_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HET2476616.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4162..5058)
                     /locus_tag="VQU28_001261"
     CDS             complement(4162..5058)
                     /locus_tag="VQU28_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HET2476617.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLLIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAIFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5067..6002)
                     /locus_tag="VQU28_001262"
     CDS             complement(5067..6002)
                     /locus_tag="VQU28_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HET2476618.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(5999..6724)
                     /locus_tag="VQU28_001263"
     CDS             complement(5999..6724)
                     /locus_tag="VQU28_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HET2476619.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(6808..7443)
                     /locus_tag="VQU28_001264"
     CDS             complement(6808..7443)
                     /locus_tag="VQU28_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HET2476620.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNNLEAIIQSVARDYPLTEIKLLSILPVNEGEEYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(7445..8272)
                     /locus_tag="VQU28_001265"
     CDS             complement(7445..8272)
                     /locus_tag="VQU28_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2476621.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8294..8630)
                     /locus_tag="VQU28_001266"
                     /pseudo
     CDS             complement(8294..8630)
                     /locus_tag="VQU28_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
     gene            complement(8631..9347)
                     /locus_tag="VQU28_001267"
     CDS             complement(8631..9347)
                     /locus_tag="VQU28_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HET2476622.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPVEVMDAIKEAGYHVLDLAHNHILDSQIEGVISMADII
                     EKAGITPIGVYTHEPRDQAPLVIKEVNAIKVALLAYSYGFNGIEQYIFQEDYNRYLSD
                     LNEDKIKAEVERAEKEADITIIMPQMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSKTVEKDGDKKLIIY"
     gene            9870..10481
                     /locus_tag="VQU28_001268"
     CDS             9870..10481
                     /locus_tag="VQU28_001268"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HET2476623.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEGIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            10526..11254
                     /gene="rlmB"
                     /locus_tag="VQU28_001269"
     CDS             10526..11254
                     /gene="rlmB"
                     /locus_tag="VQU28_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HET2476624.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFLNRL"
     gene            complement(11293..12204)
                     /gene="trxB"
                     /locus_tag="VQU28_001270"
     CDS             complement(11293..12204)
                     /gene="trxB"
                     /locus_tag="VQU28_001270"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HET2476625.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVKEIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12270..12494)
                     /locus_tag="VQU28_001271"
     CDS             complement(12270..12494)
                     /locus_tag="VQU28_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HET2476626.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(12572..13315)
                     /locus_tag="VQU28_001272"
     CDS             complement(12572..13315)
                     /locus_tag="VQU28_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2476627.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILLLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13315..14115)
                     /locus_tag="VQU28_001273"
     CDS             complement(13315..14115)
                     /locus_tag="VQU28_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2476628.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14273..14629)
                     /locus_tag="VQU28_001274"
     CDS             complement(14273..14629)
                     /locus_tag="VQU28_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2476629.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(14642..15154)
                     /locus_tag="VQU28_001275"
     CDS             complement(14642..15154)
                     /locus_tag="VQU28_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HET2476630.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15154..15648)
                     /locus_tag="VQU28_001276"
     CDS             complement(15154..15648)
                     /locus_tag="VQU28_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476631.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            15783..16229
                     /locus_tag="VQU28_001277"
     CDS             15783..16229
                     /locus_tag="VQU28_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HET2476632.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16226..16873
                     /locus_tag="VQU28_001278"
     CDS             16226..16873
                     /locus_tag="VQU28_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HET2476633.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(16894..17475)
                     /gene="pdxT"
                     /locus_tag="VQU28_001279"
     CDS             complement(16894..17475)
                     /gene="pdxT"
                     /locus_tag="VQU28_001279"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HET2476634.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(17476..18351)
                     /gene="pdxS"
                     /locus_tag="VQU28_001280"
     CDS             complement(17476..18351)
                     /gene="pdxS"
                     /locus_tag="VQU28_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HET2476635.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(18503..19882)
                     /locus_tag="VQU28_001281"
     CDS             complement(18503..19882)
                     /locus_tag="VQU28_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2476636.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKDKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20177..21100
                     /locus_tag="VQU28_001282"
     CDS             20177..21100
                     /locus_tag="VQU28_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HET2476637.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGISPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21160..21765
                     /locus_tag="VQU28_001283"
     CDS             21160..21765
                     /locus_tag="VQU28_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2476638.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKTIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLTHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            21783..>23023
                     /locus_tag="VQU28_001284"
     CDS             21783..>23023
                     /locus_tag="VQU28_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HET2476639.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRFLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGAS"
BASE COUNT         6846 a         5158 c         4197 g         6822 t
ORIGIN      
        1 aaccacttcg acactgatca tggtggtaaa gcgtaatccc ttgtttctct gatttacctg
       61 acaaaaaagt ccaatagtaa gtcagctgac tatcactttc taagacccga taagaggttt
      121 catctgcatg aagaagaggt tgttctaaca acttttctcg taaaaggtta taaagggact
      181 ctaaatagta ttgactcgcc ttgatatgcc aattagcaat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattagg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaagctg agtcaatagt ctcgttataa aggggcttta
      361 ggaataggag ctttcacaat tttatcactc ggatttttat cactgcatgc ttggcatttg
      421 taagcgtgtt ggatatgatc tatgcgtttt aattgcgcag gaataaagac taattcttgt
      481 cgttgaaggg ttgctccaat ctcttttaga tctccctgac aatcagagca aatgctctct
      541 tctacttgat gatgaacttc ttctgaatca aattgggcaa gaagagcttg gcgtttccct
      601 ttagatttct tacgtttgta ggtaatttcc tctgtttcaa ctgggaacat ctccatcttt
      661 ctcagatgga gactcttcct caaaaaggtt gagttgtcca gaggggcaaa cacttttctc
      721 agaggatttt ccatagagct tttgagtcag ataagccact tgttcacgaa gaagggcaag
      781 tgcattggtg agactatcaa ttgtagcact ctgttgttga atgattttca atcttcttcc
      841 atgggcttcc cctccttgtt ttctaccttt attatactaa aaagaaagtc ctcatttcaa
      901 tagaaatcac gactttctga tggatttatt tttggagtga tagaaaagtc cttcataagc
      961 cagtctactt gttcagatgt gagagctttg acatcctttt ctgtactggg ccaagtcagt
     1021 ctgccgttcc caaagcgttt atatagtagc caaaatcctt gaccatccca gtaaagggct
     1081 ttaaagcggt atttacgtcc accacaaaag agaaaaactt gacctgagaa aggatctaat
     1141 tcaaagtggg ttttaaccag ataagccagt gaatcaatgc cttgcctcat atccgttttc
     1201 ccacatacga gatagacctg ccctaggcta gatagatgaa ttgtcataga gcaacacctt
     1261 atccaaaagc tgttctacca tttcgagatt gagagattga aaaaaactta cttcaagctt
     1321 gccaatacga attttaagca gaatatcatt tctcttttta gaatcaaaag gacgagattg
     1381 tggaatctct acaggaacaa taggttgtgg cattaaaata tcctccaata gttttacttt
     1441 actaatagta tacaggagga actccatgaa tgatagatac cttgttatga cgcgattact
     1501 atattagaaa gttggctctc taattaaatt aattctcatt atatatatca aaagaccgca
     1561 gcttgaaaac tacggtctaa ttttttgtcc aaatgtaaac cacttggaac tattattcaa
     1621 tttattcttt ttagtaaatg ttcccgtttt aatcccagtt acagttttta atcctctgta
     1681 accgttgatt ttacaaagct tttaattttt ttgcgattat tcccactcaa ctgttgcagg
     1741 tggtttactt gtaatatcgt agacgatacg gttaacatga tccacttcat ttacgatacg
     1801 tactgagatt ttttgaagta cttcccatgg aattttggca aaatcagcag tcataccatc
     1861 gatagaagtg atagcacgga ttgcaatcgt gtagtcatat gtacgaccgt cacccataac
     1921 accgactgaa cgaacgcctg tgttaacagt gaagtattgc caaatatcgc ggtcaagtcc
     1981 agctttagcg atttcttcac gaagaatagc gtctgattca cgaacggttt caagtttctc
     2041 ttcagtgatt tcacccatga cacggatagc aagtcctggt cctgggaatg gttggcgcca
     2101 tacgatatgg tctggcatac caagctctgt accaagagca cgaacttcat ccttgtaaag
     2161 agtattgagt ggttcaatca attcaaactg catatcttct ggaagaccac ccacgttgtg
     2221 gtgtgacttg atagtttgag ctgtatccgt accagactcg ataacatctg tatataaagt
     2281 accttgagca aggaatttca catctttgag cttgcttgct tcgtcatcga atacatagac
     2341 aaactcgtta ccgatgattt tacgtttttg ttcaggatca gaaacgccag caagtttgtc
     2401 aaggaaacgt ttagcagcgt ctgctttgac gatattcaaa ccaaacttac caccgagcat
     2461 gtccataact tgatcagctt cgcctttacg aagaagaccg tggtctacga agatacagat
     2521 caattgatcg ccaatcgctt tttggagaag aaccccaacg acagatgagt caacaccacc
     2581 tgatagacca agaaggacac gtttatcacc gacggtttca cgaatttttt tgatctgcat
     2641 gtcaatgaaa ttatccattg accagtcacc tttagcctta caaatgttaa gggcaaagtt
     2701 acgaaggata tcatttccgt atacagaatg acgaacttct gggtggaatt ggataccgta
     2761 aatgtgttta tctgggtttt cgatggctgc gtatgggcag tcagctgatg tacctgtacg
     2821 aacaaagtca gcaggaatct cagtaaccgc atcaccatgg ctcatcaaaa cagtctgttc
     2881 atcaggtgtt gattcaaaaa gcgctgatgg tgtgtgagtt agggttgatt gaccgtattc
     2941 acgatttcca gcatcacctg caggaacaac ttttcctcca agtttatggg tcaataactg
     3001 cataccataa cagattccca aaattggaat tccgagttcg aagatttctg ggtcaatatc
     3061 aaatgaacca tcttcatata cagaatttgg accacctgat agaataattc ctacaggatt
     3121 gacttcacga acttcagcag ctgaaatttt atggcttttt agttctgaaa aaacaccaat
     3181 ctcacggata cggcgtgaaa tcagctggtt gtactggcta ccatagtcca atacgatgat
     3241 tttttctaca tcttgcaaat cagttgaaat gttgctcatc tttttccttt ctcaaaagaa
     3301 atatcttttc tcaatattct atcacaaata agggcgaatt accaactaaa caaggcgaag
     3361 tttgactttt tctagtttat tggggtacaa tagagaaaag atagaaatga agtgaaaaat
     3421 atgctacctg cttatatgaa aatccatgat cagataaaaa aggatattga cgagcaccgt
     3481 tgggctattg gtgagaggct tcccagtgaa agagatttag ctgagcagtt tgcggtcagt
     3541 cgcatgaccc tccgccaagc cgtatctcta ttagtcgaag aaggcgtctt agagcgccgt
     3601 gtaggaagcg gcacctttgt ttccagtact cgagtacaag aaaagatgcg agggacaacc
     3661 agttttactg aaattgtcaa atcccaaggt aaagttccct ctagccagct catttcctac
     3721 aaaaaaacca ttcccaatga gcaagaagtt gccaagctag gaatttttcc aacggacaat
     3781 attatccgaa tggagcgggt ccgctatgcc gaccaagttc ccctagttta tgaagttgct
     3841 tctattcctg aaaaattcat taaggacttt aaaaaagaag aaatcacaag tcatttcttc
     3901 caaaccttgc aaaaacatgg ctatcgtatc ggcaaatctc aacagaccat ctatgctcgc
     3961 cttgctaaag aaaagattgc ccactatttg gaagttgaaa aaggacatgc tattcttgga
     4021 ttgacccagg tttcctacct agaagatggt actgcttttg aatacgtaaa aagtcaatat
     4081 gtaggcgaac gctttgaatt ttatcttgaa aataattaga ttaccacgca aaaactctct
     4141 gtcacggaca aagagtcttt tttatttttc taagaataaa tctttcaaag aaatcaaagt
     4201 tacagcaaac aatatcattg ccataccaag aaaatcaatg ggataaaatt gttcattcat
     4261 tattaagaag gcaaagaaaa tcgccgatat tggctcaatt gaagccaaca agcttgactt
     4321 gaccggtcct atcagactgg ctcctttaag gaaagctgta taggcaaaga cagtcccgat
     4381 aaggataatg cctgcaaacg caaggagaaa atcaagacta gtcgggatat cggcctgtag
     4441 aacccctgta aaaggaaggg cgaccaaacc tgctatgacc attcccacac caatgagcaa
     4501 gctgctcccc cactttttaa tcaaggctat gggtaaaatg atatacagag cataagtcaa
     4561 ggcagaaaag agaccccaga acagaccagc aggtgtcatg gataactggt ccaactgccc
     4621 atgtgttgcg atcaggaagg ttcctccgat ggcgaatatg atggaaacta tctctcccag
     4681 tgtcggtgcc accctatcct tgatacagct ataaattaaa attccgacag gacaaacata
     4741 ctgaagcacc gtcgctgttc ccgcattggt ctcctgaata gcagacagat aggcgaattg
     4801 gttgagaaaa agaccaatca gagcaaaaat aagaagagac agcaaactct ttctatcctt
     4861 taaaaagacc agtattttat cctttgcagt agcataagcc aagagcatga gaattccacc
     4921 agcgattaaa agacgcaagt tggtcaagac cagagccgaa attccgtgtg ccattaggta
     4981 ttggccactc gttcctgaca acccccaagc aataccagcc acaactgtta gtaaagtccc
     5041 ttttaaacta tttgacatgt ctctccttac tcttctttgc gaatcaagtt catcacttga
     5101 ttgcggtcta atatccattg agctctctcg tccttttcat aggtacgatt cgataggaaa
     5161 atagtggctt cttgcttctg acgattccac atgataaagg tacctgtata gcccgtatgg
     5221 tctagccaat ctccttccaa attccatgct aaagaacgtt ccttgtcatc caaaggagaa
     5281 aaattttgat ttaagtctct tgcaaaatca tctgctaaat agtgttctaa aaagatttgt
     5341 aaatccttta tagtcgaaaa taaaccagca ctcccagcat gtctacccag gagacgagcc
     5401 ttgggatcat gcactatgcc tgcctctaca cctctaactg ttggaacagc aagctcaact
     5461 ggcccaaact tggtttccgt cattccccaa ggtttccaga cttgatcctt taaaatcaca
     5521 tccaaatctt gattaaaaat tctttccaaa ataaagccca acagcaaaaa atggacatcc
     5581 gaataaagaa aggctggctg acttcgtctg ttgagatgaa acatcgcttc ctttaattca
     5641 ggggctgtta aaagatcacg attaggaata aaaggatcaa ggtctgttgc atgagtcaag
     5701 agctggcgaa tagtgatgtc tggataatca ctctcaggta aaaaatctat taccagtcta
     5761 tcaatatcta attgacctat ttcccacaag aaggtacaaa ctgtgccaac cccaacaacc
     5821 ttgctgacac tagctagatc ataaactagt cctgcctcag tctgcaagcc atgctctggg
     5881 tcactctggc ctaaatagaa ctctgtccat tgattgtcct taaaatacgc aaaagaggct
     5941 ccgggataaa ttcctgcctc gatttgttct tctatttttt taataatctt ggtccacttc
     6001 atacttcttc aaaccacaat tcaacattat ttctatcttt accaaggaag aatttttcag
     6061 acttaggaat aaaatattca gtagactcaa acttctggcg aagacttgct atgtctaatt
     6121 cattgaccaa gaacttgagc atagataagt cccaggtaac cgtattgtcc acagtcaaat
     6181 cctgcccctg agctggaata aaatcaaggg atgccccaat ttcagatgat tccaagaaac
     6241 tttcgatatc agttgggaga tgtaattcca tagaaatctc aaatttactt aaagaaattg
     6301 atgccaaatc tgtttgaaat tcaggctttt ctcctacttc tactagactt gctctgtcat
     6361 cttccgcatg aatcaaaatc aaatcatctt ctggtgagaa aatttcaaaa gcgtagccat
     6421 tttgaccttt atataatcga tgaatcgaat ctgttttaga taagattcct tcaatttcta
     6481 agggattttc caccttgaca atcaatctag ctagtttttt tcttccctct accttacgag
     6541 tacgcatact gggagcttct tctaaaacca gcttttcaag acccgtttgg tcacctagtg
     6601 acagaaaggc cgattcttct aacaaggcct tcattccaag ggtttcaata taaaatgttt
     6661 catttaattt tctattatta gcttttaaag taggaataat ccgtacaatc tgatttacat
     6721 tcataaattc ctccgtcact ttttatttta aaggatttta gaatttttta caagatatta
     6781 tgctcaaatt taacattatt tagctattta gtaaagatag tctttcaagg attttgacaa
     6841 agcctgataa ccagcaatac tgaggtgcag tccatcagtt gtatattctt ttttgagttg
     6901 gcctgcttgg tctgtcaaac aatcaaatac tggcacaaat tccacctgca tataggcaga
     6961 tgcaagctct tgataggctt gattccagtt ctgaattttt tcattcgagc ggatatagac
     7021 tgcctgctgg tactcctctc cctcattgac aggcaaaatg gaaagcaatt taatctctgt
     7081 caatggataa tcgcgagcaa cggattgaat gatagcttcg agattattga gagcctcatt
     7141 cacaggaaca tcctttccga tatcatttgt cccaatcaga agaaaaattt tatctactgc
     7201 tccaccatat agatgagcat caagattctc tagtaacagt cctgtctgat agccacgaat
     7261 tcctcgattg acaatcgtct ttgaagtccc aaatagctcc tgtagaggat aatactcgac
     7321 aatggaatcc ccaataaaaa gaatgtttgg ttctacaaca gaaatgtgat ttaggtgacg
     7381 atacttagtt tgaatttttt cttgttcctt taggagccaa ttttctaata actgtactgc
     7441 cacttcattc tcctttttct aaccagtttt ccaagacttg ataaactcct ccttggctgt
     7501 tggctggtgc tagagcagtc gccacagctt tggctttctc atcagcattt tccatcgcat
     7561 aggcaattcc agccatttca agcatttcaa catcattttc actatcacca aaagccatga
     7621 tttcttggga tttcaagtcc cagcgcttga gtaattcctc caagccccat gctttatgaa
     7681 tcccagcttg gaggatatca atgcaaccat agccactgga tacagctcgg acacggccat
     7741 caaagagagc attgatttct tccaaaaccg aactcaaacg ttcctcacca acaaccatgc
     7801 tcatcttgag cacaccacca aagaggtcag atgttaattc atccacaaat tgcatccgtt
     7861 ggtagaattt ttcaatcatt tctggagtca taaaactttc aagatctgta aaaatcgtac
     7921 cttccttgac aaaatcaccc ttcatccccg ttacaacaaa ctggtcctga cacgctcgac
     7981 ccttgaaatg agccaaagcc ttgttgacaa tggcgtcatc ccatgtctga gcctgaatca
     8041 attcattgtt ttcaaaaata cgagcgccat tagcaacaac cagaaccact cgatccacca
     8101 agggactcag tagttgtctc atgcggtgaa tttcattgcc cgtcgcaatg acaaaacgaa
     8161 tgcccctttg atccaactga tctaaaatct tttccaatcg tgggagatca agctgacccc
     8221 tagcatccag caaggtccca tccatatctg tcgcaattac cttaatcatc atttactttc
     8281 ctctggattc aagctaatac cacttcaatc ccacatgttc attcatttct ttataggctg
     8341 tatcaattcg ttccttagtc gcttcatcta actggtcacg atgactgcca cccctctata
     8401 aaatcttcca aaatataagt ttggtaatga tacaagggat atccttctgg tgaaaaggtt
     8461 ccctttggtg ttcgattgac ccaagtagga tgagctttag ctgttccgat agttgttttt
     8521 ccatccttct tcttgatggt gacatccatg agaacaccac gttcagtcca cttagcattc
     8581 tcttcatctc ccatagattc aatccgttga ttggaaatga agttccccat ttaatagata
     8641 atgagtttct tatctccatc tttttcaacc gttttagatg gttcaacaac gtgaggatgc
     8701 cctccaaaga taatatccgc tccccaatcg atcatcttgt gataaagagc tttttgttct
     8761 tcagttggtt ccaatcgata ctcaacaccc atctgaggca tgataatggt gatatctgct
     8821 tccttctctg cccgttcgac ttcagccttt atcttatctt cgtttaaatc tgaaagataa
     8881 cgattatagt cttcctgaaa aatatactgc tcaattccat tgaaaccata ggaataggct
     8941 aacaatgcaa ccttgatagc attcacttcc ttaatgacca gcggagcctg atcacgtggt
     9001 tcgtgcgtat aaactccgat tggagtgatt ccagctttct caataatatc ggccattgaa
     9061 ataactccct caatttgcga atccaaaata tgattatgag ctaaatccag cacatgataa
     9121 cctgcttcct taatagcatc cataacttca acaggagcat taaagagaag ataacccgct
     9181 aaataatgat ccttattaat ggttccttca aaatcaccaa tagctaaatc tgcttgcttg
     9241 agccaaggag tcacatactc aaaattttca tgaaagtcat aggtaccgtc ttctttttta
     9301 gctgaaaaga aaagtccatc gtggtagagc aaatccccgt gggccataat tctggcagtt
     9361 ttttcctctt cctactcctg agacttttgc ttagtcccct cttgagaaat agtatctcgt
     9421 ttttgactag tcaagggatt ccttggaagc tttcaaacaa caagaccaag cccattgata
     9481 aaccaactgc tagcaagagt atcgccacaa atcccttatt gctccacttg cgataactcc
     9541 taaaaagttt accaagccct tcataaaacg aaaagctaaa ccaccctgat ttcgattttg
     9601 tcttctttgt atcttcattc tccctacttt cttatgcaag ccttttcttt ttattatatc
     9661 acagataagt atttctttca caattgaatt gaacttccca tctattttct ataaatccta
     9721 aatgccataa tgctttcaat tcttgtcatt ttgtgatatc atgtagaggt aatgaaagga
     9781 aatgaactaa tccacagtgg cttattccaa gtataccact tgggctttgg cagtagttaa
     9841 ctgcactaaa cataatataa ggagagaaaa tgtctgcaat agaacgtatt acaaaagctg
     9901 ctcacttaat tgatatgaac gatattatcc gtgaagggaa tcctactcta cgcgcgattg
     9961 ctgaggaagt cactttcccc ctatctgacc aggaaatcat cctaggcgaa aagatgatgc
    10021 aattccttaa acattcccaa gatcctgtca tggctgaaaa aatgggactc cgcggtggtg
    10081 ttggactggc tgctccccag ttagatatct caaaacgcat tatcgctgtt ttggtaccta
    10141 atattgttga agaaggcgaa actccacagg aagcctacga tttggaaggc attatgtaca
    10201 atccaaaaat cgtctctcac tctgttcaag atgctgctct tggcgaagga gaaggttgcc
    10261 tgtctgttga ccgtaacgtg cctggctatg ttgttcgcca tgcccgcgtt actgttgact
    10321 actttgacaa agatggagaa aaacaccgta tcaaactcaa aggctacaac tccattgttg
    10381 ttcagcatga aattgaccac attaacggta tcatgtttta cgatcgcatc aatgaaaaag
    10441 acccatttgc agttaaagat ggtttactga ttcttgaata aagaaaatcc cgttgcaaga
    10501 cggggttttg tgttataata gaggcatgaa aacaaatgat attgtctatg gtgtccacgc
    10561 cgttaccgaa gccctccttg caaatacagg aaacaaactc tacctccaag aagatctccg
    10621 aggtaagaat gttgagaaag tcaaggaact agctacagaa aagaaggtgt ccatttcttg
    10681 gacatcaaaa aaatctctct ctgagatgac tgaaggtgct gttcatcaag gttttgttct
    10741 acgagtgtct gaatttgcct atagcgagct agattacatc cttgcaaaaa cacgccaaga
    10801 agaaaatcca cttctattga ttctagatgg tctaaccgat ccccataatc tgggttctat
    10861 cttgcgaaca gccgatgcga ccaatgtttc aggtgtcatc attcccaagc accgtgctgt
    10921 cggagtaact cctgtcgttg ccaaaacagc cacaggtgct attgaacacg ttccaattgc
    10981 ccgagtgaca aacctcagtc aaaccttaga taaactcaag gatgaaggct tctggacctt
    11041 tggaacggat atgaacggta ctccttgcca caagtggaat acaaaaggga aaatcgccct
    11101 catcatcgga aatgaaggaa aaggcatctc tagcaacatc aaaaaacagg tcgatgaaat
    11161 gattaccatt ccgatgaatg gacatgttca aagcctcaat gccagtgttg ctgcagccat
    11221 tctcatgtac gaagtttttc taaatagact ataaaaaagt ttccagtcat ctgattggaa
    11281 acttttttat gattaactat gttctgtgat aaacttgtag gcttcttgac cagcgatagc
    11341 tccatctcca actgctgttg ttacttggcg aaggtctttc aagcgaacat ctccaactgc
    11401 aaagataccg tcgactgcag tcttcatgtg gctatctgtc acaatccagc ctgcctgatc
    11461 ttggatattc aattctttaa caaaatcgct aagagggtcc aaaccaacat agataaagac
    11521 accaccgaag gcttgctctg tcacttgacc tgttttcaca ttttcaaaaa cgacagattc
    11581 tactctgttt tcacccttga tttccttcac tacagaatcc cagataaagc tgattttttc
    11641 attcgcaaag gcgcgatctt gtaaaacctt ttgggcacga agttggtcac gacggtgaac
    11701 aatggtaaca gtcttagcaa aacgagtcaa gaagagggct tcttcaacag ctgaatctcc
    11761 accaccaact accaataaat cttggtcacg gaagaaagca ccatcacaca cagcacagta
    11821 agaaacacca cgactgttca gttcttcttc tccaggcact cccaaaggac ggtgtttaga
    11881 accagttgct acgataactg tacgtgtttc atatgtttgg tcatcagtca tcactttctt
    11941 aaaatcacca tggtcttcga cattttcaac ataaccataa atgtgctcaa caccaagatt
    12001 ttcaagtggt tcaaacatct tttcagccaa ttcaggtcca ctaatattag cgtatcctgg
    12061 gtaattttcg atatcagatg tattattcat ctgaccacct ggcagaccac cttcaatcaa
    12121 agctactttt agattgcttc gagcagcata caaggccgca gtcatccctg caggtccagc
    12181 accgataata atagtatcgt acatatagat tccttctttc ttgttgtaac tatctttatt
    12241 ctaactcttt cttgtcaatc acgcaaggat tatgccttga aaacaagaat taaactcata
    12301 accgcaaagg ataatactgg gacaaccaag actccaatca taatcatatc atagagatgg
    12361 gcttggcgag ccctggctgt cttatcaact cccgacatgg ctctcagacc aatccaaatc
    12421 cctaaaaaaa tcagaacaag gatggtggtc aagatcaaac tctcgaaata taaagaaaat
    12481 agttgcagta gcatgatttc tctcatttct atctttttta aagagtaaac tcagctagtc
    12541 caactaactg agttttcctt tatctattat atcaaatata agtccgtttg taactagcga
    12601 agaattcttt tgtccgctct tctttagggg tgtggataat ctcatccgga gttccagact
    12661 cgatgatttt ccccttatct aataagagaa ttttatccgc aacttgggct acaaaggaca
    12721 tgtcatgact gaccaaaatc atggtctgac ctgacttagc agcatctgca atagactttt
    12781 ctacttcacc gaccaattct gggtcaaggg ctgaagttgg ttcgtctaag agcaaaacat
    12841 ctggtttcat agcaagcgca cgcgctaggg caacccgttg cttctgtcca cctgataaat
    12901 ggcgaggata atggttttca cggtccgaaa gcccaacctt agccaactct tccttggcaa
    12961 tcttagtcgc ttcttggtca gataatttct tgacaacaac caagccttct ttcacattat
    13021 caagtgctgt tcgacgttca aataaattaa actgttggaa aaccatagac aacttacggc
    13081 gtagggcaag gatttcttct tgagtgattt tagaaaaatc aactgaaaaa ccatcaatct
    13141 gaatagagcc actgtcaggt gtttcaagat aattgagact gcgaagaaag gttgattttc
    13201 cagctcctga agaaccaatc aaggctacaa cttccccttt ttgaatatcc aagttcagat
    13261 gatccaagac agtctgtcct gaaaaggatt tgcttaaatt cgaaatctta atcattaacg
    13321 aaggtctcct ttcacatctg tttgcactgt atcaggtgca gaaatagcca tttttctctc
    13381 gatgaaacga ccgaggcttt caattccgat attgactacc caataaacaa gggcaacaga
    13441 gatgaagcgt tcaaaatagc gataatcagc tccacctaga atctgagctt gggcaaagac
    13501 ttccacaaca cccgcactaa aagctagaga tgttcccttg gtcaaaccga tgagggaatt
    13561 aatcaaggtt ggagtagcta ccaccgctgc attaggaata atcactcgtc gataaacttg
    13621 cgctcgggtc atacccagac tgcgcgccgc ctcaatctca ccaggattaa ctgagagaat
    13681 ggctgcacga atggtttcac tagcataagc tgcctcatta aaggcaaaag cgacaatcgc
    13741 aaaagctgca gctggaatcg cattgatatt gagaccagtt ccccattgct gattgagggc
    13801 tttcaaagcc aaagggattc cgtagtaggt caacatgagt tgcaccaaaa tcggtgtccc
    13861 ttttaagaaa ctaacaaaga aggcctgcaa gggatataaa atcttgacac gattgatctt
    13921 cacaatggca aaaagaagcg ccaaaaccaa gccaaaaagg gcaccaccaa ttgtcaacat
    13981 aattgttgtt ggaagttgtt ggacaattct agggattcca tcaaagaccg aacgtaggct
    14041 aaacagcttg ccatccggaa tcaattgcat caagttttgg taccaatctg atgctaaaat
    14101 cgttgtaaca ttcataaaaa catcctcttc tgataatgat tcattctacc atgcttctgc
    14161 cgtcaatgaa attatttgct acggtcagat acagactttg tctacctact agtttatcat
    14221 actttgaaac agggaagtta tatattttat ctatcaaaga gatagataaa aactatttca
    14281 gtcccagttc ttcataagat tttcgataac cgatttgctt aacagttcca ttttctacta
    14341 aaatgggccg ttttaacaac ataccgtcac ttgctagcaa ctcagccgct tcttggtttg
    14401 acaaacttcc taccttatct tttagcccta attcacggta tttgatacca ctggtgttga
    14461 aaaattgctt caattcaaat cctgaggttt ctagccaatt caaaatgact tcttggctag
    14521 gtgtttcttc cacgatatgg acggctttat agtccacacc taattgattt aattcttgtt
    14581 ttgctttttt acaagttgaa catttggggt attcgataaa ttctaacatg tgcttctcct
    14641 tctatttgtt tatatcttta aaatctacgc cttcatgctc caagagccaa gctttctttt
    14701 ccactcctgc agcatagcct gtcagacgct tgcctgctcc caacacacga tgacaaggta
    14761 ctaggataga ccaaggattg cgtcccactg ctccaccaat tgcttgagca gaagccactt
    14821 gcaggtcttg agcaatttgt ccataggtca ctgtctgacc ataaggaatg ccctgtaaat
    14881 agtcccaaac tctcttttca aaattcgttc cgattggcgc caagagcaag ttggataaat
    14941 cctgaggctt gcctttaaag taatcatcta agcaagcaat aactgggtct aaaataggat
    15001 gactaacaac ttcttctatc gtttcatctc ctagtcccct ctcaaaatgc ttctgctcct
    15061 gaacccaaat tccatacaaa taatggtcat cagctacaag tgatagaata ccaattggtg
    15121 aggagtagag tatttttaca tacttctttt gcattatttc tttaattttt gataggccaa
    15181 gcgttcgcca tctactggca ccttaccaac ttgtttaaaa ccaagttttt caaaaatatg
    15241 ttgcataacc ttgttttcag catgcgtatc tgagcgaaaa tcaagataat caaaaccttc
    15301 aatcaagccc tctaagaagg tttgagcaac tccttttccc tgcacatctg ctgccacagc
    15361 aatacggtga aagactagat actctgactc tccagcttgc cagtttccct cataaatagc
    15421 ttcataggcc tcctctggac tcttggtcac agcagcatag gctagcagtt ctccctcttc
    15481 caaggctacg taggcttgcc ctgagatgat atcatcgata ataacatctg catttggata
    15541 gccattttgc cactggtcac taccagcatc tgctaaacat tttttggcat cctccatcac
    15601 ctgcatgatg gcatctactt catttggaaa agctaaacga atttccatct tttttcctca
    15661 tttctgacta gtttctccca tcatagcata atttaagtat ttctacaagc agttaaaact
    15721 tgactttgtg tttttgttat tttataatct agttatcaaa actatataaa aaggagttga
    15781 aaatgaaaac taccttttcc tacccaaaat gggcagaaat tccaaacatt gacctctatc
    15841 tggaccaggt tttgctctat gtcaatcagg tctgcgcccc tatctctcct aataaagaca
    15901 agggcctaac agcatctatg gtcaacaatt atgtgaaaaa tggttacctg acaaagcctg
    15961 acaaaaaaaa ataccaacgc caacagattg cccgtttgat tgctatcaca accctcaagt
    16021 ctgtattttc tattcaagaa atagcccaga cacttaatac tctacaaact caagcaagtt
    16081 cagaccaact ctacgatgct tttgtggact acatgaacca aggaattgac ccagctaacc
    16141 ctattatcca aaccagctgc caaaccgtca aactctatca tcaaactcta gacttaatcg
    16201 atcatactca agaggaggta atccaatgaa cactagtctt aaactcagca aacaactcag
    16261 ttttggagag gagattgcta atagcgtgac ccatgctgtg ggtgcagtca tcatgcttat
    16321 cttgctgcct atttcatcca tctatagtta tgaagcacac ggatttttat cctctatcgg
    16381 cgtttccatt ttcgtcatca gtctctttct catgttccta tcatccacca tttatcactc
    16441 tatggcctat ggttcgaccc acaaatatgt tttgcgaatc attgaccatt ctatgattta
    16501 cgttgccatt gccggctcat acacgcccgt tgtcttgacc ttgatgaata actggtttgg
    16561 ctatctgatt attgtcatcc aatggggaac gaccatcttt ggtattctct ataaaatctt
    16621 tgctaaaaag gtcaatgaga aatttagcct tgctctttac ctgattatgg gctggttggt
    16681 tctggctatc attcctgcca ttatcagtca aacgacaccc gttttctgga gtctcatggt
    16741 aactggcgga ctctgttata cagttggagc tggattttat gccaagaaaa aaccttattt
    16801 ccacatgatt tggcatctct ttatcctagc tgcgtccgca cttcaataca tcgctattgt
    16861 ttattacatg taaaaaagtt gagaaattca atctcaactt ttttctttac acatattgat
    16921 aaagtactgg tgcaagcgca catcatcagt caattctgga tgaaaagaac ttaccaacat
    16981 atttttttct tgggctgcaa caatttgatt gttcactgtt gctagaattt ctacaccctc
    17041 accaacacta ctgataatcg gaccacggat aaaggtcatt ggaatcttgc caactccctt
    17101 acattctgct tccgtgtaga aacttcctaa ttggcgccca taagcattac gctcgaccac
    17161 catatccata gttcctagat gactctcttt ctgagaagtg atttccttag ccagcaaaat
    17221 taagcccgca caggtcccaa acactggtaa gccagataga atggcttctc ggatgggaag
    17281 tagcatgttc tggtcacgta agagcttgcc catggttgta gactcaccac caggcaaaat
    17341 caaacccgac aagtcactct gatcttgctg aaaatcatct agatttctga gttctacact
    17401 ctcgacacct aattgatcta gcacttttgc atgttctgca aaggcccctt gcaaggccaa
    17461 tattccaatt ttcatctatt ttcctcgttc agccatgaga atttggattt cattttcatt
    17521 aataccaacc atggcttctc ctaaatcttc agagatttga gctaggattt gaggattacg
    17581 gaagttagtc acagccttaa caatggcact cgctcgttta acaggatctc ctgacttgaa
    17641 aatacctgaa ccgacaaaga ccccctctgc ccctaattgc atcattaacg cagcatctgc
    17701 tggcgttgca acacctccag cagcgaaatt tacaactggc aattttccat gttcatgaac
    17761 atattggacc aattctacag ggacttgcaa atccttggca gcaacataaa gctcgtcctc
    17821 acgtaagttt tgaatgcggc gaatttcctg attcatcata cgcatatgac gaacagcttg
    17881 gacgatatcc cctgtccctg gttctccttt ggtacgaatc atggaagcac cttcagcgat
    17941 acgacgcaag gcttcaccca aatccttagc accacagaca aaaggaactt ggaattcttt
    18001 cttgtccaca tggaaacggt catcagctgg agatagaact tcactctcgt cgatataatc
    18061 aatttcaata gcctctaaaa tctgagcttc aacaaaatgc ccgattctga ccttagccat
    18121 tactggaata ctaaccgctt cttggatttc cttaatcatc tttgggtcgc tcatgcggga
    18181 aactcctcca gctgcacgaa tatcagccgg aattcgttcc aaggccatca cagctgccgc
    18241 accagcagct tctgcgatac gagcctgttc aggattctgc acatccataa taacaccacc
    18301 cttgagcatc tgtgccaagt ttttatttag ttcataacga ttttcagtca tttgttttat
    18361 cctcatcatt tgtattggta gctacctttt cattttaagt cagaacagaa tgaagcacaa
    18421 gataaaaaaa ctataattgt atggggacag attggctaaa aactatctga ccttaactta
    18481 aggccagata gctcattcat ttttattttt cagccgtaag ggcagccatt gtgatgtagt
    18541 tgtatggttt attgaagtgt ggcaagaaga agaggtctgt caatgccaat ttatcaattg
    18601 tcacatgctc ttggatagca agtgagaaca tgtggattcc catgctaatt gcaatatcat
    18661 gtgaaaccat ttgggcacca agaatttcac ggctatcttt gtcaaagaca atcttaattg
    18721 ctacttcatg gttgtcatgt ttcatgaatt ctggtttttg aagatcgtta aagcctgttt
    18781 cagttgcgtt gtaaccagca gctttcgctt tttcaagagt caaaccagtt gaaaccatgt
    18841 gaagaccgta gattgagata ccatttgaac cttgaacacc gattccttcc aattcatgtc
    18901 cacaagcgtt gtaggcacca acgataccag tgcgaacagc atttgaagca agagcgatat
    18961 agcttgtatc tttacgagca ttgtcataaa cagtcgcaca gtcaccaaca gcgtaaacgc
    19021 ctgggataga tgtttcttgt ttcttgtcta caaggaaggc accgttgcgg aagagttcga
    19081 tcttaccatc agcaagggct gtgtttggac ggaaaccaac tgcaaggata accatatcca
    19141 cgtcaaagct ttctttgtca gtaatcaagc gttcaacttt accatcacct tcgattgctt
    19201 taacagtttg acctagagcc aagcggatgt tgtgatcttc caagttcttc gccatcattt
    19261 gtgtgaagtc tttgtcatag taaccgttca agacagtatc aacgatatca acaaggacaa
    19321 cttcttttcc aagacgttca aaggcttcag caagttcaac accgatgtaa ccaccaccaa
    19381 caacggcgat acggtcgaga tgttggctct tgtcagaaag tttattgata acttcttcag
    19441 cattttggta caatttcacg aattgtacgt tttcaagagt tgctttaaat tcgcggtttc
    19501 ctttaacaat ttcaacacct tcgattggtg gcaagattgg tgtagaacct gtagcgaaaa
    19561 tcaatttctc gtatgattct ttgtgttctt ttccttcaac ttctgctgta accactttgt
    19621 tatcatagtc gattgaaaga actggtgaat tcatataaac cttagcacct ttagcttcta
    19681 atttgtcttt atcagaatag aacaagcctt cagcaccgtc aatttgttca ccaatccaaa
    19741 gagccattcc acatcctagg aaagagatgt tagagttttg gtcaaataca acaatttcgt
    19801 tctcatttcc aaaattatcc aacatggtat tgatacatgc tgtaccagcg tggttagcac
    19861 cgactacaac gattttactc ataaaaaatt tcctgccttc attctaaatt tactttacta
    19921 gtataccatt taccgttagc gtttacaagg gggggactat tatttcagca ttttttatca
    19981 aaaataattc cccatttatc ctgttttcaa gcctcatgaa ttttatgagc atatttcttg
    20041 actttttcca aattttgttt gtgaaaattc taactttatg gtattataat aacatgaaaa
    20101 taaaatgtaa gggcttcaat ttagatgatt atttgatatt gcaacttatt ttctataaga
    20161 gaaaggagtt ggtagcttga ctcttcgttc acattctgaa cggctaatgg ggactactat
    20221 cactatttca ttagtggatg agcaagccga tatctttctc caaaaatcct ttgacttgct
    20281 caaagagctt gaataccgct tcaacgccaa tagtcaagaa tctgagttga tggaaatcaa
    20341 ttatcaagca ggaatatccc ccgtcacagt tcatccagac ctgtttgagc tgatttcact
    20401 tgggttagag catagcctag cgctctctag ccacctcaat atcagcattg gtcccttaat
    20461 tcaaacctgg cgtatcggtt tttcagatgc caaggtcgcc cagcctcaag aaattgaatc
    20521 ggtgctgcct ctaatcaatc ctcatggtat cgagttagat tcttctactt ctactgtgtt
    20581 tttaaaacag aaaggaatga agatcgatct aggttgttta gccaaaggat acagtgcgga
    20641 taaggttgcc caatttctta ggaaagaggg ggtgacttct gccttgatca atctgggagg
    20701 gaatatcctg accattggaa aaaatcaggc aagaggggat aacccatggc aaatcgggat
    20761 tcaagaccca gccaatccta ggggaaatca tttaatgacc atccctgttg tcaataaatc
    20821 tgtcgtgact tcaggcattt atgaacgtca cctgaccgtc gatggacaag attaccatca
    20881 catttttgac agtcaaacag gatatcctgt tgaaacggaa ctagcgagtc taacaatcat
    20941 ctctgataaa tcagtcgatg gcgaaatctg gacgactcga ctctttggag aaagacctgc
    21001 ttctatcctc tggcaagtcg aaagtttgga gggcatcgaa gtcatcctca tcgataaaga
    21061 aggccaccta agctgttctt caggaattcc tactctatag agacaactgt ttcaatggaa
    21121 ttttaaaatt gtattatgta aaaagagaaa ggaaatatca tgttaaaact tattgctatt
    21181 gttggaacaa attcaaaacg ttctacaaac cgtcaattgc ttcaatacat gcaaaaacac
    21241 tttgctgaca aagctgaaat tgaacttgtt gaaatcaaga ccattcctgt cttcaacaaa
    21301 ccagctgaca agcaagtacc tgctgaaata ttggaaattg ctgctaaaat cgaagaggca
    21361 gatggcgtta ttatcggtac tcctgagtat gatcactcta ttccagctgt tttgatgagc
    21421 gctcttgctt ggttgtctta tggtatttac ccacttttga acaaaccaat catgattaca
    21481 ggtgcttctt acggtacgct tggttcatct cgtgcccaat tgcaacttcg tcaaatcttg
    21541 aatgctcctg aaatcaaggc aaatgttctt ccagatgaat tcttgctcac acactctctt
    21601 caagcattta acccaagtgg cgacttggtt gaccttgatg ttatcaagaa attggatgcc
    21661 atctttgatg acttccgtat cttcgtgaag attactgaga aattgcgcaa tgcgcaagaa
    21721 ttgcttcgca aagatgctga agactttgac tgggaaaatt tgtaagatag gagaccgaaa
    21781 gaatgaaatt tgttggactt gttggatcaa actacgatca atcatataac cgcaaactct
    21841 tggaatttat ccgtcgcaat ttcaaattca aatttgaatt agaagtactt gaaatcgacg
    21901 aagttccaat gtttaaccaa gacgaaaaat gggacgaaag cttccaattg cgtttcttgt
    21961 ataacaagat tacacgtgct gatggtgtca ttatcgccac tcctgagcac aaccacacta
    22021 tctcagcttc actcaaatct gtacttgaat ggctttcata cgaagttcat ccatttgaaa
    22081 acaagcctgt tatgattgtg ggtgcatcat actatgacca aggaacatca cgtgctcaag
    22141 ttcaccttcg taaaatcctt gatgctccag gtgttaatgc ctacacgctt ccaggtaacg
    22201 aattccttct tggtaaagct aaggaagctt ttgataacaa cggaaatatc accaacgaag
    22261 gcactgttaa attccttgaa acttgcttag ataactttgt taaatacgta ggagtcgttt
    22321 cgaaattgaa aaaaccaaaa ccaatcgaat cagaagactt ggattgtgga aaaccaatcg
    22381 ctacaaccat cacagaagtt gaccctgacg atccagaatg ggtagaaaaa gttgcagcaa
    22441 tcactggtgc tgtttctggt gatacctatg tcaaattgga ccacggtatc ctaacagtta
    22501 accaaattga tatgttcttg aaagctatgc catttgaatt gacatacgct gacgacaaca
    22561 accaattcct ctactacaac aacgctcacc aagatccaga cactatgttc gctaaacgtg
    22621 taccacctca atcaggtagc cgtatgtcga ctgttcatgg ttctcttcca ccagcacgta
    22681 tgaagaacgt agagtgggtt atcggaacac ttcgcaacgg aaaccaagaa tacgtccgta
    22741 cgatcgttcc aggttctcct gcaggtgtta tcaacactca caactaccaa gcaatgtact
    22801 atcctgatgg atcatacgct ggtatcaatg aaatcgtctt taacttccaa ccatggcttg
    22861 actggtacct aaaagagact ggtcaacgtt tggtgggtgg tagcggacca tttgctcctg
    22921 ctgccggagg tcatggagac gccgatgcta cttctggcgc ttctgattca ggggatgctg
    22981 gaggccacgg tggtgacgca gacgctacag ccggtgcaag tta
//
LOCUS       DAVBCB010000034        22997 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVBCB010000034 DAVBCB010000000
VERSION     DAVBCB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.034"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(20..667)
                     /locus_tag="VQU28_001285"
     CDS             complement(20..667)
                     /locus_tag="VQU28_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HET2476640.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(776..1294)
                     /locus_tag="VQU28_001286"
     CDS             complement(776..1294)
                     /locus_tag="VQU28_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HET2476641.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRKWKKIVL"
     gene            complement(1521..2390)
                     /gene="rsmI"
                     /locus_tag="VQU28_001287"
     CDS             complement(1521..2390)
                     /gene="rsmI"
                     /locus_tag="VQU28_001287"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HET2476642.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGTSAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2393..2710)
                     /gene="yabA"
                     /locus_tag="VQU28_001288"
     CDS             complement(2393..2710)
                     /gene="yabA"
                     /locus_tag="VQU28_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HET2476643.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2750..3640)
                     /locus_tag="VQU28_001289"
     CDS             complement(2750..3640)
                     /locus_tag="VQU28_001289"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HET2476644.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEMAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3637..4275)
                     /locus_tag="VQU28_001290"
     CDS             complement(3637..4275)
                     /locus_tag="VQU28_001290"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HET2476645.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVKVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4495..5292)
                     /gene="proC"
                     /locus_tag="VQU28_001291"
     CDS             complement(4495..5292)
                     /gene="proC"
                     /locus_tag="VQU28_001291"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HET2476646.1"
                     /translation="MKIGFIGLGNMGASLTKSVLQARPSDEILLANRSQVKVDAFIAN
                     FGGQTSNNEEIFEEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSEIFCQLLAKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5296..6558)
                     /locus_tag="VQU28_001292"
     CDS             complement(5296..6558)
                     /locus_tag="VQU28_001292"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HET2476647.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAVSFLPRLEQ
                     VLVADRKEAGLEPIQFRLDSKASQFVSGQAAQAQDFDTEFLDYILAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVTGDGQIRE"
     gene            complement(6568..7698)
                     /locus_tag="VQU28_001293"
     CDS             complement(6568..7698)
                     /locus_tag="VQU28_001293"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HET2476648.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMVEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGVFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRFQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7781..9661)
                     /gene="cbpE"
                     /locus_tag="VQU28_001294"
                     /gene_synonym="pce"
     CDS             complement(7781..9661)
                     /gene="cbpE"
                     /locus_tag="VQU28_001294"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HET2476649.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIDGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYKGQW
                     YYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWLKKAN
                     DWYFYKTGGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVPIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSVNKSE
                     SE"
     gene            complement(9664..10551)
                     /locus_tag="VQU28_001295"
     CDS             complement(9664..10551)
                     /locus_tag="VQU28_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2476650.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10541..11002)
                     /locus_tag="VQU28_001296"
     CDS             complement(10541..11002)
                     /locus_tag="VQU28_001296"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HET2476651.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMFLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(10999..11907)
                     /locus_tag="VQU28_001297"
     CDS             complement(10999..11907)
                     /locus_tag="VQU28_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HET2476652.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11900..12097
                     /locus_tag="VQU28_001298"
     CDS             11900..12097
                     /locus_tag="VQU28_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476653.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12149..12820)
                     /locus_tag="VQU28_001299"
     CDS             complement(12149..12820)
                     /locus_tag="VQU28_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2476654.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12874..13095)
                     /locus_tag="VQU28_001300"
     CDS             complement(12874..13095)
                     /locus_tag="VQU28_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HET2476655.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAARF"
     gene            13367..14176
                     /locus_tag="VQU28_001301"
     CDS             13367..14176
                     /locus_tag="VQU28_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2476656.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHITAIHKAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14357..15232)
                     /gene="aguB"
                     /locus_tag="VQU28_001302"
     CDS             complement(14357..15232)
                     /gene="aguB"
                     /locus_tag="VQU28_001302"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HET2476657.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKLLAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYNLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15242..16327)
                     /gene="aguA"
                     /locus_tag="VQU28_001303"
     CDS             complement(15242..16327)
                     /gene="aguA"
                     /locus_tag="VQU28_001303"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HET2476658.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALERPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVV
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16324..17451)
                     /gene="nspC"
                     /locus_tag="VQU28_001304"
     CDS             complement(16324..17451)
                     /gene="nspC"
                     /locus_tag="VQU28_001304"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HET2476659.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17451..18710)
                     /locus_tag="VQU28_001305"
     CDS             complement(17451..18710)
                     /locus_tag="VQU28_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HET2476660.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMLD"
     gene            complement(18707..19567)
                     /gene="speE"
                     /locus_tag="VQU28_001306"
     CDS             complement(18707..19567)
                     /gene="speE"
                     /locus_tag="VQU28_001306"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HET2476661.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEYDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            19948..20537
                     /locus_tag="VQU28_001307"
                     /pseudo
     CDS             19948..20537
                     /locus_tag="VQU28_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(20503..21978)
                     /locus_tag="VQU28_001308"
     CDS             complement(20503..21978)
                     /locus_tag="VQU28_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HET2476662.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            22685..>22997
                     /locus_tag="VQU28_001309"
     CDS             22685..>22997
                     /locus_tag="VQU28_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2476663.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTKIKRRTVRKCLGKGRFKEVYSADYAQQSYENNRKHSVKRSSVTKELKEKILHYHNQ
                     KF"
BASE COUNT         6746 a         5245 c         4095 g         6911 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaatg taattctcaa
       61 gctgtgagtt cattatgatt tgtaaatccg atatatcttc agcagctaaa aacacatttt
      121 gaaatgttcc aagtccgttt tcaccaaaac tatattctct cttactatac ttactcttat
      181 tattctctat atagttagag tcaaggttct taacctcttg aagttcattt tctttacttc
      241 tgaggttaaa atcttttact tctgaagtta agttttttga cttctgtaag tcattttctt
      301 ttacctgaaa tatactcata aagtctttaa cataaatgat gttcggctta ccaagtccaa
      361 gccttactct ttcgatcagt cctattcctt tttttacatc aagctcatct aatgttttta
      421 tggcagttgg ctttgagata tttcttcttt tcataatttc ttcgacagta aaatagataa
      481 atactctgcc ttccttgtct atccagttat tcttaaatga cattcctgtc tgtttaagaa
      541 gtatggaata aaggataatt gcttctgctg ataagccccg aaattcttct ctatcaacta
      601 atatctccgg tacttttaag aagttaaatc tttccgcttc tctgttataa aaatattcaa
      661 agtccatgca agatcacctc ccttcttaaa aatttgcaaa gaaaaagacg atagtttttc
      721 ttctatcgcc tttggtaacc atttttatga aattttttaa agttaaaaat ataatttata
      781 atactatctt cttccatttt ctgattttag ttctgaaagt tccaaaaggt gcaaccgtat
      841 ttacatgaat aaacttatac acttcccaag tcgctgtttt agtcgcttca tcagcccact
      901 ttctcatatg cggttcaaat aattcttcct cactcaacga atcaatcatt gcagagatag
      961 aattaatatt ttcatttaat tttgctttca actcttgcag agataaatga gcgtaggtat
     1021 ctgtgaacca ctgatataat tcaccaagtt gattccattt aaatttatcc gatggtgttt
     1081 ttacttgaag cccctttctt tcatcttctt cccatttaag aaccaagttg gtccaaccaa
     1141 cctgataagc aaggttttct gctggagttc tgtcaacttc atcagctctc ttatctttta
     1201 aattttctgg aatattatca aattctaaaa tatatttctc aaatgttttc tctatctcag
     1261 cttttagttc ttctttattt tcgtatgttc gcaaaaaatc accttctttc tgatttatca
     1321 atcctttgat ttaaaatgac cttatttttc tatttatacc ttctaagaac gctataactg
     1381 ctgctccaaa catcggtatt ccgtatgggc ttaataaaaa acttaatacc aaagcttcta
     1441 taccaagtga tacatcttta tattatcatc actaattaag ttaaacagaa acagacagaa
     1501 ttattacatc ctgtctccct ttattgtttt tcttcccaat cgtggtaggc agcgtagagt
     1561 tgactcttat tccactggta aatcttagct acttccttaa tagcttgatt tttcttcatg
     1621 ccttgctgga tacgggcttg gatttctaag aacaagtcct cctcgtcttt ttcctccaca
     1681 tcctgactgg caccttcaac aataagaagg cattcgccct tgagtggcgt ttcagcgatg
     1741 ctttccagta attcagaaat tgtacctcgt tggtattctt catagatttt ggtcaattcc
     1801 ctgaccaaaa caaccgagcg gtcaccgtag acttctaaca tattttccaa cgtgtctgct
     1861 acacgatgag gtgattcata aaaaatctgt gtttcaggat aatctttttt agagccaaaa
     1921 aattgcttct gttgacctga ttttctcggt aaaaaaccgt aaaagatatg tggctgtggc
     1981 gctaaaccac tggcaatcaa ggcagaaatt cctgcagagg tacctggaac agtcacaact
     2041 gcaatttctt cctcaatagc tgccttaact aaatcatgac cagggtctga aatgctaggc
     2101 aaaccggcat cagagacctg agcaatactt tgccctgctt tcaagaaacc aatcaaatca
     2161 ggaatttttt ccttggcatt gtgctcatga aaactgatct gcttggtgga aatgtcaaaa
     2221 tgcttgagca aaagccctgt attgcgcgta tcctcagcag caatccagtc cacttctttc
     2281 aaggtctgga tagcacgaaa agtcatatca tctagattgc caatcggcgt tgccactaga
     2341 tacagcttgc catagggaga ctgcccctta aaactttttt gaatttgcat gcttactccc
     2401 tgtataacaa ctcatcacag aacatacatt cttcgtcctg ctctcgacgt tgtccataaa
     2461 aatcattaca tacgtgaaat ccatcacggt aaatacgacg gacactttcg cgaacatgct
     2521 tggccttgac gggagcgtct gcttccacct cacccaagcg ttctcgcaac ttactatttt
     2581 ccaagcgaag agctgtattt tcttctacca agctcttgag atttttcttg atggcttcca
     2641 catcggctaa agttaccaat aattgttggg aaaaatcatc cagcgcgtca aataattctt
     2701 ttttgtccat aaaacagccc tttcctttct ttatcattca tttttgagtt tatatttctt
     2761 tcaagaccag atattccatg gcattttgaa agctgacatt agcttgccac atttttctag
     2821 cttctagtaa atcttgtaga atcactctta ctcttacctg caagaggtcc tgcccacaga
     2881 gaacttcaag aatccgtaaa acctgatcct gtttttcctt atcatctgcc aagttggcta
     2941 atttggcaac ctgtagataa ctttcttttt tcttagctac taaccaagtc agcaggcgtt
     3001 cactttcatc gaccaaggtc caaaaacttg cctgattagc caacttttct gcttcagctc
     3061 gcgattgact aaacttagct aaaagagtcg cttttttctt aacaagtccc atttgttcta
     3121 agagtaagat aagtttttct tcttgctttt taaagtggaa gatctgagtc cgacttcgga
     3181 ttgtcggtaa catcttttcc tcatcgctag tcaagaagaa aatataaact tcactctggg
     3241 gttcttcgat gaccttgagc agagaattgg ctgcgttggg atgcatttta tccgcttgct
     3301 cgatgataaa gacctgttgc tggctttcaa tccctgcttg agaaaactgt cccaccaatt
     3361 cccgaatacg ttctgtcttg atgacctgat tgactggctt aatcaaggtg acatctggaa
     3421 actcttcctg ttcaatcagc ttgcaacttc ggcatttctc acatggtaag acgccaactt
     3481 tatccgtaca aaagaggctc ttagctaaaa attgcgccat ttccaagctt ccaaagaaac
     3541 ctgaaaagag ataggcgtga ttgagctggt cttgttctaa gatacggaca aaacggtcaa
     3601 actgagctgg ttgccaagcc tttagttgat cttgtttcat ttggccaagc ccattccgtc
     3661 aaacaagaca gccttggtag tttccacaac ttgctccaaa gggagactag catcaatctt
     3721 gacaatgcga tttccctctt tatccagaag agaaaggtag ccttgacgaa cttttttatg
     3781 caagtccaac ccttccaaat ccaaacgatt aacctcgcgg tcactattag cagcaatacg
     3841 agccagccct tcttccacct cgatgtcaaa atagagtgtc aaatcgggtt tgaggccatc
     3901 tgtcgcaaac tgattgagcc agtcaatggc ttcaatatct aagccacgac caaatccctg
     3961 ataggcaaca gaactatcga taaaacgatc catgatgacc aacttgccag cttcaagggc
     4021 tggaagaact ttttccacca aatgctgtct gcgactggca atatagagaa gtagctctgt
     4081 tttagcatcc atctgagtat gacttggatc caaaatcact tcccgaatct tctccccaat
     4141 caagactccg ccaggttcac gggtcgtcag cacctttact cctttttcct ctaaaattgg
     4201 tagcagagcc tctaaaacac tggtcttgcc tgctccctct ggtccctcaa gagagactaa
     4261 aaatcctttt gacatgtcta actcatttct tttttactac ttctattcta tcaaaaaaat
     4321 aggggtttgt gacaatcttt ttgtcttctc atttcatact caatgaaaat caaagagcaa
     4381 actaggaagc tagccgcagg ctgtacttga gtacggcaag gtgaagctga cgcagtttga
     4441 atttgatttt cgaagagtat cagccaccat aaaagaggca gataaaacta cctcttattt
     4501 acctagttct tgtgttcgtt tataggcttg atgaactgcc tccatgactg tgcctcgaaa
     4561 agcatgcgct tctaggcttg ctacgccagc aatagtcgaa ccgcctggac tacaaacttg
     4621 atctttcaaa actccaggat gttgctggct ttctaggacc aattgcccag ctcctaccac
     4681 agtttgggct gccattttca aggctgtttc tcgtggtaat cccgtctgaa cacctgcatc
     4741 tgccaaggcc tcaataaaga gatagacaaa ggctggtcca caacctgcaa gacctgtcgc
     4801 tgcattgatt aagctttctc ctaattcaac caagagacca gccttggcta aaagctgaca
     4861 aaagatctca ctgtcctcag ccctgcaatt aggagacaag gcataactaa tcactccttg
     4921 cccgatagaa gcaggggtat taggcatcat acgaataatt cggtgttggc ttgggataag
     4981 acttgctagt ttttctaagg tcaatccagc tgccatcgaa atcaaaagaa gactttctct
     5041 tttttcaagg atggtctggt attgagaaag cagttcagaa aactgagcag gcttaacacc
     5101 tagaaaaatc acatctgctt cttcgaatat ttcttcattg ttagaagtct gaccaccgaa
     5161 gttagcgatg aaagcatcca ccttgacttg actacgattg gcaaggagaa tctcatctga
     5221 cggtctagcc tgcaagacag acttggttaa actagcaccc atattcccca aaccgataaa
     5281 tccaatcttc atctcttact cccttatctg tccatcacca gtgaccacat acttgtagct
     5341 agtcaattcc ttcaagccca taggaccacg cgcgtgcaat ttctgagtag aaatccccat
     5401 ttcacaacca agaccaaatt gtcctccatc tgtgaaacga gttgaggcat taacatacac
     5461 cgctgcagag tccacttgat ctgtaaagta tgctgcagct tcagcatttt ccgtcacaat
     5521 ggcatccgaa tgatgggtac tgtgggattc aatatgcgca accgcttctt ctaaactgct
     5581 cacaacctta acagctagaa tatagtctaa aaactcggta tcaaagtctt gtgcttgagc
     5641 agcttgacct gaaacaaact ggcttgcttt gctatctagg cggaattgaa ttggttccaa
     5701 cccagcttct tttcgatctg caaccaacac ttgctccaag cgaggaagga agcttactgc
     5761 tttgttttca tgaaccagca gaacctccat ggcattacaa acagaaggac gactggtttt
     5821 agcattgttg atgatagaca gcgccttgtc ttcgtctgca tccttatcca catagacatg
     5881 gacaatccca gttcctgtct cgataacagg tacaatcgca ttctcaacca ctgcattgat
     5941 caagccagct cctccacgag gaatgagaag gtctagatag cccttggcct tcatcatagc
     6001 ataactactt tcacggctag tatcctccac cagttgaatc acatttggat gaatagtagt
     6061 cgtctccaag cccttcttca aggctgtgac aatggcatgg gttgtttgat aggcatcctt
     6121 accactacga agaacaaccg catttccact cttaagagtc aaagcagccg catcagacgt
     6181 cacatttgga cggctttcat agataatacc gatgacaccc atagctacac gtttttttgt
     6241 gataaccaaa ccattttcaa gctgacttgt ttctaaaact tcaccgattg gatctggtaa
     6301 ggcaaccact tcacgaattc ctcttgccat cgcttctata cgatctgcat ccaaataaag
     6361 acgatccaac atcacatctg agattttccc cttagccgct gccatatcga gggcattagc
     6421 cgctaaaatt tcctcagtag cagccactaa gtgatcagcc atggctagca aggcttggtt
     6481 tttcacttct tcactagctg tgttaatcga ttttttaaca gcctgtacct gttcaaattg
     6541 ttcttgtcta ctcaccatag tttacctcta aaattctgta aaaagtagtt ggatttcagg
     6601 agtaatggaa atccagtcgt cacggtaaat caagacaccc ttggcttttt gaaaacgcaa
     6661 catatcctcc aaagcagatg ctccaaattg cacgcgtcct tttccaagtg attttccact
     6721 ttccttatca aatactgtca cgatatcacc gtaagaaaag actccttctg cttcaacgat
     6781 accagataag agaagactct ttccatgttg agagagagct tccgcagccc ctttatcaac
     6841 ccaaatagaa ccttgactct gagcatagaa ggcaagccat tgtttctggg tacgaagccc
     6901 cttctcttga gcaacaaagt aagaaccatc ctcggtctcc tctgccgcct caaccatgga
     6961 atctgatttc aaggatgagc agatataaac aggaactcct gattccgtcg cgatagttgc
     7021 agccttgatt ttggttaaca tacccccagt tccgtttgac gaaccagctc caccagccat
     7081 atcaataatc tcacgattga tggtctcgat tctctccaag cgtttggctc ttggatctga
     7141 attaggattt ccagtataga gaccgtccac atctgtcaag aaaactaaaa ggtctgcttg
     7201 gaccatcgcc gctacttgag cacttagagt gtcattgtcc ccaaccttga gctcatcaat
     7261 aacgacacta tcattctcat tgatgatagg aattgcccca cggttgagca aaaccgacaa
     7321 agcctgatgg gcatttttat aacgacgctt atccacaaag tcatcttggg tcagcaagat
     7381 ttgtgcagaa acgatttgac gcaagagaag attggtcgta tattcttcca acaaaagtcc
     7441 ctgccctacc gctgctgaag cctgtttatc agcaatttta gtcggacgct ttttaaatcc
     7501 taaggctcca aaaccagccg caatggcacc tgaagacacc aaaatcaact catgaccagc
     7561 ctcgtgcagc atagccaact gctgggtaat atcctttacc ttactacgtg ataaacttcc
     7621 atcctcattt gtcagagaag aagtgcccac cttaaagaca atccgtttgt atttcatagt
     7681 ctcactccga ttattcatat ttatccatta taacatgaat aacaggaaat agaaaagagt
     7741 tgataggaaa aagaaggccc taaaaccttc ttttctacta ctattctgat tccgatttgt
     7801 ttacactcgt ttcagagctt tgtgaagttg aggatgtcaa atcttgacta gttgaagaat
     7861 ttgtaacact ttcatgttga ctcgtttctt tattttcaag atcctttttc actacttctt
     7921 tggattcttt tatttctgaa tgacttgtag ttttaattgt agcagatttc tcgatcggaa
     7981 catccactaa ccaggcacca cttgaatcca cagtatagcc ttctggtgtc ttaccattca
     8041 caagtaattg accattttct ttcaagaagt accatttatc cttgtcttta atccaaccta
     8101 tagctcgtga accacctgtc ttgtagaaat accaatcatt tgccttttta agccagcctt
     8161 gcttcatagc tcctgattct tctagatagt aatgatgtcc agcaacttct atctcacctg
     8221 tcttcatgat accagtagaa tccatatagt accaggcttc tttatctttt acccagcccg
     8281 ttttcatttc ccctgatgga gcaaagtaat accattgccc cttatattga agccaacctg
     8341 tcttcataga gccatcaaca tccaaataat accactgctc tttatcttga acccaaccaa
     8401 tttccatctg attttctttg ttaaaataat accagattcc atcaattttc ttccaatttt
     8461 tagcagaagc accagagtct gtcaaataga accaatgatt gttccatttt ttccattgat
     8521 tctgtaacaa gatacccgtt tggttaaagt aataccattc accttcgatt tcattccaac
     8581 cgacagcata ctctcctgta gaatcaggcg cttgatacca ccagttccca tatgcactct
     8641 tatgccaacc agcttgaaaa cttggaatcg gcttgtagga tgttgaaata ttgacaaaac
     8701 cgtcttttcg aatatcaaaa actgttgcat catagtcttt gctggctgcg ttgattctct
     8761 caattcctcg ttctttgagc caattaacat actcactatc aacaccattt ttccaaggta
     8821 gactatccga agtttgaaca atcaaactcg gactcaaatt tttaatgaaa tccttggtat
     8881 ttgatttgtt ggtatcatgg tgatgattaa acttcatcaa atcaactttt ccaatgagag
     8941 gaccatactt gtcttctgct ccatgaacat tatctaaatc gcccccaagg taaattttct
     9001 tgccattgac tttcaccacg ctaatcaagg aattggaatt gtcatcccaa attttcttta
     9061 attcacccga tgaatcagtt tcattttcat aattatagag ctgaatatcc atgtccccaa
     9121 actgaaaatg agcatcccct tgtgtgatat tttgaataac tgaaacacct ttttctgcag
     9181 cagtctgtaa aaccttatca tagccataca gattatccca tagacgttca gaattagtaa
     9241 tacgactatc actatatttc ttaagataga ctcggtcaac tggataggta gacagtaatt
     9301 catcaacatt tccaatatga tcactgtggg tatgggtcac caaaataaaa tcaagttttt
     9361 ggacacccaa ttccttcaaa cgacgaaaga cacggtctgt tagaacatgc ttataagacg
     9421 tttcaattcc ttctctccat ggataacgag aatcacttcc atctgggaaa tcataatctt
     9481 ctcctgtatc caccatggca aaatgtccat tgctttcaag aataatcgca tcactgccac
     9541 cttcttgaac attgataaag tggattttat ttcctgaact ttcttgagcc tgaacattcc
     9601 cataccatga caaacctaaa aaagcgccta caagtgctaa actagttaat ttctttttca
     9661 ttcttacttt ctcaatctct ccaaggtttc cttaaaaatc tctgggatat ctgctttaaa
     9721 ttccaaggtc ttacctgttc tcggatgagt aaaacctaaa gtcttggcat gaagaaattg
     9781 tccatgtcct ttcaaagtct tgcgaggacc atagacctca tcaccagcga ctggatggcc
     9841 gatataagcc atgtggacac ggatttgatg agtgcgccct gtctccagtt gcaactctac
     9901 taagctataa tcgccaaagc gttccaagac gtgaaaacgc gtcactgcag gcttcccttt
     9961 agcagttaca gcctgtttct tacggtcttt ttcactccgg ccaatcggcg cttcaattac
    10021 accacgatca ttaggcagat ttccatgaac aatcgcccaa tatttgcgga gagacttttt
    10081 atctttgagt tcttgggcaa gtactagatg cgcatcatcg tttttagcaa tcatgagaag
    10141 acctgacgta tccttatcaa tacggtgaac aatccctgga cgcagaaccc cattgatacc
    10201 cgacaagtcc ttaatatgat acatgagggc atttactagg gttccactgg tatgaccagc
    10261 actcgggtgc acaaccattc cctgaggttt gttaacgaca gccacatcct catcttggta
    10321 gactatttct agcggaagat cctcagccac atactctaat acctctggtt ctggcacatg
    10381 gtaagtgacg acatcaccct cttggactgt gtatttagct ttcttgactt gaccattgac
    10441 caagacctgg cctgatttaa tttgttcatt cgcgagacta cgtgataatt ctgacaaatc
    10501 tgacaaagcc ttatccaaac gcagaccacc agtttcaatt ttaatttcca tttatttcct
    10561 cttttagcat tgcaatcaat aaaataatca ctccaaccgt cagatagcta tctgccacat
    10621 tgaaaattgc aaagttgata aagtcaaggt ggaacatatc cacaacaaag ccctgactga
    10681 ccctgtcaat aaagtttcca agaccacccg cgattattag agtcaaaccc aagaacatcc
    10741 agaatgagtc ctccatgtgt ttatgtaaat accaaatggc acctatcacg acaaccagag
    10801 taatgacagc gaataacagc tgctgatctt gtaagataga aaaggctgca cctcgatttt
    10861 gcaggtaggt caagctaacg aaattgggaa tccaggagcg cacttcaccc agtggaatct
    10921 gctggacgat ataggatttg accaactgat ccagcccaat taaaagcagt acaatgactg
    10981 ccactattgc tctttttttc atgatttcct cttttgatca aaatattctt gcatgacttc
    11041 tacgaagaga gtcccagctt gactaagctc cacttcttca cgtttaacat agaccatgcg
    11101 gttatctagg ttatccttga gacgaataac tgtaatgcca ttaacactgt cactatctaa
    11161 aaatccagaa cctgtcgcat aggcgtccgt ccgctccaaa ataccattca aggtggcacg
    11221 gtctgtcaca ttaaacatct gtgagctagc gctggtatcg acaaagttct ctgaataata
    11281 aaggtactcg tctttctctt gagtgaaacg aaccgttggt aaatccgcta aatcctccat
    11341 gactaattcc tctttctggg ctaaaggatg accctcacag agataaatat gggtatggaa
    11401 aggaatcaat tcgatgacct ccagacctaa cttttcaacc cgttgcataa tccccttttt
    11461 attttgattg ttgaggtaga taatcccaat ctcactatgc ccttgcgcca cttcatctaa
    11521 tatttgaaca gtagttgatt caaaaatacg gaagttctta tagtcaggat agcgctctga
    11581 aaaggccgta atagttggtg gtaagaagtc atagtgctgg ctagcaacgg aaaattcatc
    11641 tttttcttct tcaggattgg catactgatt ttgaaaaata tcaaatcctt taaccaattc
    11701 ttgcgctttt tcataaaatt ccatcccacg acgggtcaag aaagtccctg agctggtccg
    11761 acggaaaatc ttaaagccca actctttttc caaatcacga acagaaatag acagactcgg
    11821 ctgactaaca tacatctttt cagcagcttc acgaaaagta ccactattgg caatagccac
    11881 aacatagcgt aattgttgaa tgttcatctt ctacccccaa cttctttatc ttttcattat
    11941 accatatttt agaagttttc caaaaaggaa aaaagaacat cctattcttc ttaactatct
    12001 tcactatctg cctttctcac gccaatctta ttttcaaaat caattcaaaa aaataagtgg
    12061 ctacacacca cttcagtatc ggaaagaaaa acgttgacta tttgtgaaaa aagaaaatgc
    12121 cggaaaattc cgacattttt ttagtaagct aacttcctga aaataggagt aaccaaacat
    12181 cccagaaggc agtgatattg atgagaatat gaactagtat tggatagtag agatttttag
    12241 tcatgcgata caatagagct aaaatcagac ctccactagc ataaatgaaa aaagcaagag
    12301 gagttaaagc aaaattgata tgaatataac cgaaaataat agcagaaagc aaaacatctc
    12361 cgtaccaagg tgagtttttg aaaaaggttg tcataagcac acctcgataa atcaattcct
    12421 cagcaatagg ggcgatgaag caaacgatga gcaagaaata agggaactcc tgtctcccca
    12481 tcatttctac cgtttcattc aaagaaatct gatttgaaga cagggatatg aaatacgaaa
    12541 agaggaagtc agacatatat gaaatgatgt agcccagtaa aaggtagatg aagtacctca
    12601 gctgccactt gaaatgaaaa attttctttc ctgcaaaaac cagtagatag atgaccgcta
    12661 agaaaagaac tccgctctcc atcaaaagca gaatctgaaa aaattcacga cttgctggta
    12721 gatagggctt tgcgaggtga ttgaaaactc tccaaaacca agttgatttg taaaaaatta
    12781 aggacaatgc tagtaaaatt tgaatagccc gttttttcat attaaattct ctgctttctc
    12841 cacttatttc ttcatattac taataaattt tacttaaaat ctcgcagcac ttcctttgca
    12901 aagatgattg cctcctccaa tatatcctaa atcataggtg cctctgggac aaaatccatg
    12961 aaccggcaga gttaatactg gttctatttc ccaatcgtac ctaccattag gatttttacc
    13021 atatgaatct gctccacctt gtatcagctc taattcttca tttgttagat ttatattttt
    13081 tgtcattaaa atcatgatat atttcctcca ttattcttca gattagttga gtaatctgat
    13141 actcatacct acttacaaaa aaactattat attaagttgg ttttttaatt tgtctagttg
    13201 caacattgac aaacatagta tagcatatct ttgcgaaata tcctcttcaa atcatgaatt
    13261 gtcatcaaaa catcttaaac tataaaatca attagtctca agctttctat caatttcttc
    13321 tccaaaatat gctataataa tagcaaaaga taaagaagga agacctatga ttaaactact
    13381 agccttggat atggacggca ccctcctcaa tgaagccaag gaaatcccac aagctcacat
    13441 tactgctatt cacaaagcca ttgaaaaagg tgtcaaactg gttctctgta cgggtcgccc
    13501 ccttttcggt gtcctcccct actacaaaaa actgggactc gacctccaga atgagtatgt
    13561 gattgttaac aacggttgtt caactcacca gactagcgac tggggcttgg ttgactggca
    13621 agaacttagt ccagctgaca tcgaatacct ctatgacctt gctgaaaaga gtgatgttca
    13681 gttgacactt tttgacgagt cacattattt tgttctcggt ggaaagccca atcaagttat
    13741 tgaaaatgat gctaaactag tattttcaga cctgactgaa atttctcttg aggaagcgac
    13801 tagtggaaag ttccggatgt tccaaggtat gtttttagga acaaaagaac aaacagacga
    13861 ttttgagcag cgttttgctg aagagctttg ccaacgattc agtggagttc gttcgcagcc
    13921 tgtcatttat gaagcaatgc cacttggaac gacaaaggct actgctcttt cacgactagc
    13981 tgagattttg aagattgatt cctcagagat tatggccatg ggcgatgcta ataacgatat
    14041 cgaaatgctc cagtttgcag ggcttgggat tgcaatggga aatgccagcg attatgtcaa
    14101 atctcttgcg gatgccgtta cctcaagcaa cgaagaagac ggcgttgcgc gtgctattga
    14161 gaaatatatt ctataaaaaa gaaaagcaaa tagacagaag ttactgctat ttgctttttt
    14221 gctaatattt taaaatacgg actaatcaat agagaagaat agcgaatcat aaaaatcgat
    14281 ttactagatg ccatagtatc ttatagttgc taataagaag ttagtctagc agaatgaatc
    14341 tctcatttct cccacactaa tctgtaattt gtctatacat ttctggtctt ctatctcgaa
    14401 acaagcccca gtttaggcgt tcacttgctc ccttgtctag gttataagta gctaacagaa
    14461 cagcttcttc ttgtctttca gctcgttcta gaatagctcc tgtttcatcc gtcataaagg
    14521 aggaaccgta gaagtcaaga ctggaactct gtccgccatt ttcctcacta ggagtaacct
    14581 cctctaaacc ataacgattg gctgcgatga ctggaacaat attcgctgct gcgtgccctt
    14641 gcatagtacg ttgccagtga ccacaactat ctgtatccaa aattggctct gaaccgatag
    14701 ctgtaggata aaagagcaat tcagcaccat tcaatgcaag acagcgcgct gtttcaggga
    14761 accattgatc ccaacagata ccgataccaa tcttagcata gcgagtattc cagaccttga
    14821 aaccagtgtt accaggcgtg aaatagaatt tttcttgata ataatggtca tctggtatat
    14881 gggtctttcg ataaacgccc agcacttccc catctgcatc aatgacggca atagagttat
    14941 acaagacatt accatctttt tcatagaaac tgattggtaa aacaacttgt agttccttag
    15001 caagcaactt aaaatgctga atggcagtat tttccgctac agattgggca tactggtagt
    15061 agtcatactg acgttcctga cagaaatagg gatgttcaaa caactcgggc aagagaataa
    15121 tttgggctcc ttgctcagca gcctgacgta ctaaacgctc tgcggtttgg atatttgttg
    15181 ccacatcctt agcgcattgc atctgaatgg ttgcaactct tacatttctc atctttttct
    15241 cctattctgg aatttgttgg gtgatacagt ggatattgcc accacctaag agaatatctc
    15301 tggctggtat tccgacaact ttacggtctg ggaaacactt gctgaggata tctaaggcca
    15361 cttggtcgtt tacatcctca aactgtggaa ccaagacagc cttgttggcg atataaaagt
    15421 ttacgtagga agctgctagt cgttcacctg cgtatcgctc ttcttctcct tcttcataga
    15481 tgtagcctgg caaatcttct tctgtcacaa cttgtcgaac tgcagggata ggcaatttat
    15541 gaatggtgaa gtgacaacct tttgcatctg tttcctgttc taagagttcg agatctgctt
    15601 ttgacatggc atactgggga tcattttcgt catctgtcca agccaaaaca acctcagcag
    15661 gaccaacaaa ggcaacaaca ttatcgacgt gttcattggt ttcatcctga taaataccat
    15721 aaggaagcca aataactttt tcagcaccaa gactttctaa taatgtgttt tcaatctcct
    15781 ctttagtcaa gttaggattg cgaccaggac taagcaagca actttcagtt acgagaatag
    15841 ttccttgacc atcgctatgg attgcgcctc cttccagtac aaaaggttta gcatcataga
    15901 caggcctttc caaggcctca gcaaaacgac tggctacttg gtcatcctct tcataatctt
    15961 gataaagacc atcataggtg cctccccaag cattaaaggc ccaatccacg gctaatttct
    16021 tacctttatc attgacgaga atggttgggc cagtatcacg cgcccaggca tcattggtgg
    16081 gaatgtctaa ataaacaacc ttgtctccaa gataggattg ggcttcagat agataggcct
    16141 gctccaccaa aagatagact ctttcccctt ctgctatggt ctcgatgatc tgagtaaatg
    16201 ctcttttagc agcctttcct tgaaaaggcc atgatcctgg tcgagtcggc catatcatga
    16261 gggtaccatg atggggttcg tactctgctg gcatgtgata gcctaatttt tttggactgt
    16321 ccatcatgat aatctccctt taaagtcttg atagccaaaa gctttgagta agctacagtc
    16381 tccctgttcg tccatgagat agagacttgg caatccaata ccattaaagg tattattttt
    16441 gacaaaagaa taaatggcca tgtcttgaaa ataaagtctg tctccgattt ggactggatt
    16501 ttcaaaacta taatcaccaa tcacatcgcc agtcagacag gtattagaag aaagtctgta
    16561 ggtatgggct ttttcctgtg actcaaagcc atttctcaaa ggtggacgat agggcatctc
    16621 aagtacatca ggcatatggc aggtcgcaga ggcgtctaaa accaagattt ccataccgtt
    16681 ttctacaata tctaatacct cagttgctaa ataacccgca ttaagcgcaa tggcttcacc
    16741 aggctcgata tagatttcaa gattgtaagt ttttcggata cgcttgattt ctgaaatcag
    16801 caaatccaca tcgtaacctt ctcttgtaat atgatgacca ccacccatat tgagccattt
    16861 tacctcatgt aagtagggac caaactgttc ttctactgct ttcaaagttg tttgtaaatc
    16921 atctgctccc tgctcgcaaa gggtatgaaa atgaagtccg tcaaccaaat ctagcaaatc
    16981 gctcggaatc ttgtctatag taactccaaa gcgagaacca ggtgcacaag ggtcatagag
    17041 cgcgtgatct ccttgagttg aacactgagg gttgaggcgc aaaccaacac tgacaccagc
    17101 ctctcgacaa cgagccccat gcttacgcaa ctgtctctct gagttaaaga cgatatggtc
    17161 tgttatccca agcaattcct ccatgtcctc atccttgaaa gctggtgcaa aaacatggac
    17221 ttcaccagga aattcttccc ttgccaattt ggcctcatag agtccactag ctgtcgtacc
    17281 tgatagatac tggctaatca agggataagt tttgtagagg gaatatgcct tctgggcaag
    17341 caagaccttg caaccggcct cttcttgtac atattgtaga atgcggcaat tagcttctaa
    17401 cttggccaag tcaataacat aggctggtgt tggtacttgt tctaacttca ttagtccaac
    17461 atttgtggat tttcaaccac aacccatggc aaaccatact cattcaaagc ttccatgaat
    17521 ggatctggat ctaactcctc aaggttatac actccagctt gtttccaagt tccgttcatg
    17581 actaattttg tcccaatcat ggctggaacg cctgtcgtgt atgaaattgc ttgtgacccg
    17641 acttctgcat agcactcttg atggtcgcaa acattgtaga tgtagatagt cttttcaacg
    17701 ccatctttga cacctgtaaa gatacatcca atattggttt tacctacagt acgtggacca
    17761 aggctagcag gatctggaag caaggctttc aaaaattgaa ttggaacaat ttcttggccg
    17821 ttaaagttaa tggtatccgt acgaaggagt ccaacatttt caagacattt catgtgcgtc
    17881 aagtaagatt gaccaaaagt cataaagaaa cgaatgcgtt tgacacctgg aatgttcttg
    17941 gccaatgatt cgatttcttc atggtgaagg agatacatat ctttttgtcc aacttgaggg
    18001 aaatcatact cacgcttgat agacatagct tcgacttcga cccatttccc atcttcccag
    18061 taagaacctg gcgcagaaac ctcacggaga ttaatttctg gattaaagtt ggttgcaaat
    18121 ggataaccgt ggtcaccgcc attacagtct aaaatgtcga tataatggat ttcatcaaaa
    18181 tagtgtttga gggcataagc tgaaaagaca ctagttacac ctgggtcaaa accagaacca
    18241 agaagagcag tcaagcctgc ttctttgaat ttctcttgat aagcccactg ccatgagtag
    18301 tcaaagtagg ctgtaaaacc aagttcctta caacgttttt cgtagatagc acgccactca
    18361 gggtcttctg tgtcttctgc ttcgtagttg gctgtatcga tatagtgaac acctgttgcc
    18421 aaacaagcat ccataatggt taaatcttga taaggcagag ctacattcaa aacagcttct
    18481 ggtttgtagc tttcaatcag agcaatcact tcttcaacct tgtcagcatc aagagcagct
    18541 gtctcaatct ttgtacttgt tttgccttcc agtttagctt tcaagtcatc gcattttgac
    18601 ttggtacggc tagcaatcat aatctctgta aatgtttcgc tatcttgaca aatctttgaa
    18661 atagcaactt gggcaacgcc cccacaacca ataactagta aacgactcat ttttttcctt
    18721 cctcttcttc taaaatgtcc tcaacatact tgggcaacat aaaggctccc acgtgtaagt
    18781 ttgcagtgta gtattctgtg aaaagctggc gttttttcca gccttccttg tcaaaatctt
    18841 tgacagggtg gtattttttc gatgcaaatc caaacaacca atagccagct gggctagttg
    18901 gaatatgggc ctgataaacc cgactgattg gaaaggcttg attgaccttg cggtgcatgc
    18961 ttcggcaggc cgactcatcc tcgtcaaaga acggactccc atgctggtaa atcatgatgc
    19021 cgtcttcctt cagagctcga taactattgc cgtagaattc cttggtaaag agtccttccg
    19081 tatggccaaa tggatctgtc gcatcgttga tgataatatc atagtcatat tcgcagtttc
    19141 gcaaaaagcg tagcccattt tggtagtaaa tggtaacacg aggatcatct aggcctgcag
    19201 caaagtctgg gaaatactca cgacagacct cgaccaacat ctcatccggt tccacgatat
    19261 cgatttgttc cagttcagga tagagtgtta atacttgggc aacaccgccg tcaccacccc
    19321 caataaccaa tactttcttt ggatttgggt ggacagccat gggaacgtga acggtcattt
    19381 cattgtagac gaaatcatcc gcatctgaga acaagacatg gccatttaaa atcagtattt
    19441 tcccaaaagc tggcgtatcc aagacttcga tatcctgcca ttcacttttt ccagcgtaaa
    19501 gttgcttggc tgttctcaga gacaatttga catctggagt atgaacttca gaaaaccata
    19561 aatccatcta tttctcctta tattatattt agcgcagtta gctactgcca aagcccaagt
    19621 ggtatacttg gaataagcca ctgtggatta gttcatttcc tccaatcgct tgacgatttc
    19681 cgaaagggtc taaccacagc tttttgtttc aattggtaat gagtttgata acgcatagac
    19741 gttttatctc gtctaaggta acaaggcaaa aagttcgtgg ataaccaagt agacttgaaa
    19801 tactatatct ggaggtactc ccatgcttta tgtgggcatt gatgtcgcta aaaataaaca
    19861 cgatgttaca gccttgaatg ttcgaggaaa actgttctta aaccactcac tttttcaaat
    19921 aataaagctg gttttgaact cttagatctg tctcttcgac aactcaacca agactgtctc
    19981 atcgctcttg aaaataccgg acattatgcc tttaatctac tgaacttcct gcatgaacaa
    20041 ggatataaag tttacaccta cactcctttg ctcatcaagg aatttgccaa atcattatca
    20101 cttcgcaaga ctaaaacgga taagaaagac gctcatggca tcgctcttaa acttctttct
    20161 gaccccaatc gtgaacaatt tcaacacgat aatcggcaag tagaactaaa aatactgact
    20221 agacatattc atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgt
    20281 cttgatatca tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac
    20341 caactcttga cacgctaccc taatcctcag aaaaggattg aggcaggatt tgataagctg
    20401 atagaaatta agcgattgac cgcttctaat tcaagggctt tttgccgtgc tctaaaatca
    20461 tcgccaaatc atcaaattga aataagaaat agtttagata aactattgac ttttcttata
    20521 gtttgtcttt ctcttaataa cgttgatatg attgacctct ggatcttccg tcccttggag
    20581 ggagcaacca cgttccttgg cgaattggat atagtcgaca atttctcgtg taatgcgttc
    20641 accaggagcc aagataggaa tacctggagg gtaacacata acaaattctc cacagacctg
    20701 tccaacagaa tcatccaaag ttaaactttt tctttctgaa tagaaggctt cttgcggaga
    20761 cagcactaac tcgggctgaa tatattctcc tgctatcaaa tcttttccat ctcttgaata
    20821 gagtctctta atatcagcca gagcaccaac caagcgctcg atgtcttgga tgcggtcgcc
    20881 gatggaaata taggccaaga tattgccgat atcaccaaac tcgatctgaa tgtcgtattc
    20941 gtctcgcaag aggtcataaa cctcgatacc tgttaagcca ataccctgag tgtaaactga
    21001 caacttagtt acgtcaaaat cgcaaaccga aacaccgtct attaactctt ttgagtaggc
    21061 atagtagcca ccgatagcat tgatttcatg acgagcgtac tcggatagct caatgacttc
    21121 ctcaaacgac tctttaccac gaagggccaa gttgcgacgt gaaatatcca aactagccat
    21181 caataagtaa gaggcagatg tagactgggt caggtttata atctgacgaa cgtactcggg
    21241 attcatctgc tccccgatta aaagaagcga actttgtgtc aaactcccac cagacttatg
    21301 catggaaact gctgccatat cagctcctgc atccatagca gaaattggaa gtttaccagt
    21361 gaaatgcaaa tgcgctccat gggcttcatc tactaaaacc atcatgccag cttcatgagc
    21421 catttctgtc aaccccttta ggtctgaaca gatgccgtag taagtaggat tgttgattag
    21481 gatagcctta gcatctggat ggtcctttat ggcctgtgct actcggtcat tttcaagacc
    21541 taaagcgata ccaatcttag gatctacact catctcgata tagatgggaa tggcaccaca
    21601 tagaaccaac gcattgatag cagatttatg gacatttcgt ggcagaataa tcttatctcc
    21661 tgccttgcag gttgccagaa tcatagtctg caccgatgaa gttgttccac caatcattag
    21721 aaaggcatgg ctagctccaa aagcatctgc agccagctcc tctgcatcac gaataatcga
    21781 aataggatgg cctaaattat ccaagggttt catcgaattg acatcaatgc ctacacattt
    21841 ttctcctaag agttcgacaa gttctggatt tccccgtcca cgcttgtgac ctggaacatc
    21901 aaagggaaca atccttttct tgcgtaactt caccaaggcc tcataaattg gggcttggtt
    21961 ttgatctaac tctttcaaaa catactcctt tcaatatttt taggacctta tgaaaactaa
    22021 ggaactaaag gttgactcat gaaaaaagag caggttttca cctgctctgc aatcttctga
    22081 cgtgtttatt ttggtttctg ttgagtatgt ctgttcccga tgtttcctaa ctctctagta
    22141 gcaaatatta cgtactttat tgatcgtttc acaagatgac gtgtgctttc accacactaa
    22201 aaatttatga attaggacaa gtgtcctaac atcaacttat aaaagtaggc ttttaaccct
    22261 atcaataatg tggcttttca accacgcttc ggcattcaac cctgcctgtc cgtaggcatt
    22321 ttttactcgg gtttatattt gctagaaaac atcatctcat tttctaagct ttattactat
    22381 atcatgtttt taagaacttg taaagtattt tttgataaaa aacaaaaata taatagattg
    22441 aaactagaat agtacacctc tgcttctaaa acattgttag aaatagattt tactgtcctg
    22501 atcgatttgt tctgttcttg tttcatttta ctatatgttc gtaaatgaaa gttctataat
    22561 gaagttagcc acccagccct cctaaaaatt agatggttca actagctgat agctcttgta
    22621 ggcataagaa aacacctctg tgttatactt gttgttcaac cacaaacaca agaaaggcac
    22681 agaaatgcaa gacaattata ctacaaaagg taaacatttg acaatcgata gccgtcgctt
    22741 aatcgaaaga tggaaaaaag aaggaaaatc aaatagagaa attgcctctc tacttggaaa
    22801 agctcctcaa actatccaca ctaaaatcaa gcgtaggaca gtccgaaaat gtcttggaaa
    22861 agggcgcttc aaagaggttt attctgccga ctatgctcaa cagtcttatg aaaataatcg
    22921 caagcactcg gtcaagagat caagcgtgac caaggaacta aaggaaaaga ttctccacta
    22981 tcataaccaa aaatttt
//
LOCUS       DAVBCB010000035        22314 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVBCB010000035 DAVBCB010000000
VERSION     DAVBCB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22314
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.035"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(160..2118)
                     /gene="ftsH"
                     /locus_tag="VQU28_001310"
     CDS             complement(160..2118)
                     /gene="ftsH"
                     /locus_tag="VQU28_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HET2476664.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEETGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMFGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNREIHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2134..2676)
                     /gene="hpt"
                     /locus_tag="VQU28_001311"
     CDS             complement(2134..2676)
                     /gene="hpt"
                     /locus_tag="VQU28_001311"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HET2476665.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKAREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2680..3957)
                     /gene="tilS"
                     /locus_tag="VQU28_001312"
     CDS             complement(2680..3957)
                     /gene="tilS"
                     /locus_tag="VQU28_001312"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HET2476666.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(3954..5222)
                     /locus_tag="VQU28_001313"
     CDS             complement(3954..5222)
                     /locus_tag="VQU28_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HET2476667.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKAWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5342..5710)
                     /locus_tag="VQU28_001314"
     CDS             complement(5342..5710)
                     /locus_tag="VQU28_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HET2476668.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5703..5969)
                     /locus_tag="VQU28_001315"
     CDS             complement(5703..5969)
                     /locus_tag="VQU28_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HET2476669.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6027..9536)
                     /gene="mfd"
                     /locus_tag="VQU28_001316"
     CDS             complement(6027..9536)
                     /gene="mfd"
                     /locus_tag="VQU28_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HET2476670.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYAQHAFIHQLKENG
                     YQKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPIQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKEFKEENSI"
     gene            complement(9537..10106)
                     /locus_tag="VQU28_001317"
     CDS             complement(9537..10106)
                     /locus_tag="VQU28_001317"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HET2476671.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVKYYLQEKNFEKTMQRYNG"
     gene            complement(10177..11292)
                     /gene="ychF"
                     /locus_tag="VQU28_001318"
     CDS             complement(10177..11292)
                     /gene="ychF"
                     /locus_tag="VQU28_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HET2476672.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIIEFRFNV"
     gene            complement(11376..11570)
                     /locus_tag="VQU28_001319"
     CDS             complement(11376..11570)
                     /locus_tag="VQU28_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HET2476673.1"
                     /translation="MYQVGNFVEMKKSHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            complement(11635..12771)
                     /gene="dnaN"
                     /locus_tag="VQU28_001320"
     CDS             complement(11635..12771)
                     /gene="dnaN"
                     /locus_tag="VQU28_001320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HET2476674.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(12930..14291)
                     /gene="dnaA"
                     /locus_tag="VQU28_001321"
     CDS             complement(12930..14291)
                     /gene="dnaA"
                     /locus_tag="VQU28_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HET2476675.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14504..15262)
                     /locus_tag="VQU28_001322"
     CDS             complement(14504..15262)
                     /locus_tag="VQU28_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HET2476676.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15320..16501)
                     /locus_tag="VQU28_001323"
     CDS             complement(15320..16501)
                     /locus_tag="VQU28_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HET2476677.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16684..17163
                     /gene="rlmH"
                     /locus_tag="VQU28_001324"
     CDS             16684..17163
                     /gene="rlmH"
                     /locus_tag="VQU28_001324"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HET2476678.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17207..17280
                     /locus_tag="VQU28_001325"
     tRNA            17207..17280
                     /locus_tag="VQU28_001325"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17241..17243,aa:Arg,seq:tct)
     gene            17445..17570
                     /gene="comC"
                     /locus_tag="VQU28_001326"
     CDS             17445..17570
                     /gene="comC"
                     /locus_tag="VQU28_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HET2476679.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17591..18916
                     /locus_tag="VQU28_001327"
     CDS             17591..18916
                     /locus_tag="VQU28_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2476680.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            18913..19665
                     /locus_tag="VQU28_001328"
     CDS             18913..19665
                     /locus_tag="VQU28_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476681.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19717..19788
                     /locus_tag="VQU28_001329"
     tRNA            19717..19788
                     /locus_tag="VQU28_001329"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19750..19752,aa:Glu,seq:ttc)
     gene            19794..19867
                     /locus_tag="VQU28_001330"
     tRNA            19794..19867
                     /locus_tag="VQU28_001330"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19828..19830,aa:Asn,seq:gtt)
     gene            complement(19908..20450)
                     /locus_tag="VQU28_001331"
     CDS             complement(19908..20450)
                     /locus_tag="VQU28_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HET2476682.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20629..21396
                     /locus_tag="VQU28_001332"
     CDS             20629..21396
                     /locus_tag="VQU28_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HET2476683.1"
                     /translation="MIGISLIPDLYNIIFLSSMWDPYGQLSDLPVAVVNNDKEASYNG
                     NTMAIGKDMVSNLKENKTLDFHFVDEEEGKKGLEDGDYYMVVTLPSDLSEKTTTLSNI
                     QSTAAYQSLTSEQQTEISDSVSQNSTDSIQSAQSIVALVQDLQGSLENLQNQSSNLST
                     LKNQSNQVSPITSTSLIGLSSGLTEIQGDVTSKLVPASQSIASGVNAYTTGVDKVSQG
                     ASQLSEKNATLTGSLDKLVSGSNTLTQKSSRLTAGVG"
     gene            21481..22161
                     /locus_tag="VQU28_001333"
     CDS             21481..22161
                     /locus_tag="VQU28_001333"
                     /inference="COORDINATES: protein motif:HMM:TIGR03057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
                     /protein_id="HET2476684.1"
                     /translation="MADGSGKLAEGGTKLTSGLEDLQTGLASLGQGLGNASDQLKSVS
                     TESKNAEILSNPLNLSKTDNDQVPVNGIAIAPYMISVALFVAAISTNMIFAKLPSGRH
                     PESRWAWLKSRAEINGIIAVLAGILVYGGVQLIGLTANHEMRIFILIILTSLVFMSMV
                     TTLTTWNSRIGAFFLLILLLLQLASSAGTYPLALTNDFFRSINPWLPMSYSVSGLRQT
                     ISINKSFS"
BASE COUNT         6901 a         4110 c         3643 g         7660 t
ORIGIN      
        1 acagtccctt ggacttcttc atacaattct ttaatcataa ttttcccctt tctagataca
       61 gtctaacaga ttagaaaaca catttgttcc attttctcca ttctgttccc tttagctcct
      121 aaactgcaca aaaaagagag gctccagcct ctctcagggt tatttttcgt cattcatttt
      181 tgactttact tcatcatagg atagtgcatg agattcctct tctactgctt caggcatctt
      241 tcctgtttcg taaagagctt taatttgtgt actatccaat gtttcgtatt tcaataatgc
      301 ttctgcaatt aacttgtgaa tttcacgatt tgactgaata atttcagcag ctttatttcg
      361 tgcctcattt aataatgaac gaacctcttc atcaatttca taagctgttt gttctgaaat
      421 tgatttttga ggactctgtg caccaaacat agcatggttt ccttcatatt gtactgggcc
      481 aagtttttca ctcataccgt actctgtaac cattgcacgc gccatttgtg tcgcttgttc
      541 aaagtcgttt gaagctcctg tagtttggac attaaagata atttcttcag ctacacgtcc
      601 acccattaag ccagccaatt gctctttcat atcttcttta gatagaagca tttgatcctc
      661 tttaggaagt gcaatcatgt atccgcctgc acggccgcgt ggtacaattg taaccttatg
      721 gacaacgcga gcattcgaca agactagacc aacaatggta tgtcctgcct catggtaagc
      781 aaccaattct cgttcttttt gtgaaactgt cttatctttc ttagaaggtc cagcaataac
      841 tctatcttct gcttcatcaa tatctgaagc atcaattatc gatttattgc gacgagcagc
      901 aactaaagct gcttcattca agacattctc taaatcagca ccaacaaagc ctggagtttg
      961 ttgagccact aatttcaaat caacatcttc tgctaaaggc ttgttcttag cgtgaacttt
     1021 caagattgct tcacgacctt taacatcagg acgaccaacc aatacttttc tatcaaaacg
     1081 tcctggacgc aaaagggcag gatcaagtac atctgaacgg tttgtcgcag cgatgacgat
     1141 aatcccttca tttccctcaa aaccatccat ctcaatcaaa agttggttca aggtttgttc
     1201 acgttcgtca ttacctccgc cgagaccgac tccacgttga cgtccaacag catcaatttc
     1261 atcgataaag atgatagctg gtgctgcttt tttggcatcc tcaaaaagag agcgaacacg
     1321 actagctcca actccgacaa acatttctac aaagtcagaa cctgagatac taaagaatgg
     1381 aacacctgct tctccagcga ctgccttagc aagcaaagtt ttacctgtcc ccggaggtcc
     1441 ctccaaaaga acacctgctg gaatacgggc tccaagtttt gtgaatcgtt ttggatcttt
     1501 taagaactca acaacttcaa ctagttcttg tttttcttcc tcagctccag caacatctga
     1561 aaatcttact ttaatatctt ctttatttgc tgctttagcc ttactacgtc caaaactcat
     1621 tggattacgg ccattgcctc ctcccatatt tcccatcata gagaatagga agaagaatag
     1681 aattccaaat ggcacaatgg atacgagtag attaatccat ataccacttg aactttcatg
     1741 cttaacagtt acttctgctt tatggtcagt agcaagtttt tgcaattctg atacggtagt
     1801 atctgcagga agaatagtgc tggtaaattt ctctacctta gtaacagatg gcgtgaaaaa
     1861 ctgaatacct gtttcttctt tacttgtttt aggattttta tagacaccag aaacttcgat
     1921 aacactacca tttggttggt aagttaattc ttttacatta ccatcggtaa tttcttgtac
     1981 caactcagta tagttgattt gctgacttcc tcctgagtta ttcccagaat agaaatactg
     2041 gaatcctgtc acaaggaaaa agataaataa taaccataga aaaggatttt taattaaacc
     2101 attattttgt tttttcatta aagattattc tttctaattt gaatacactt cctctttcaa
     2161 tactccaata taaggaagat tacgataatt ttctttgtag tctaaaccat aacctactac
     2221 aaactcattt gggatagtaa agcaggtata gtctgcctca atttctacaa cacgtccttc
     2281 tggtttatcc aacaaggttg caattttaac agaagctgct tctcttgctt taaacatatc
     2341 tcgcaaattc ttcaaagttt gacctgtatc aatgatatct tctacaaata gaacatgtct
     2401 tcctttgata tcttgagtca catcttgttt aatattgata acaccactac ttgctgttcc
     2461 accatggtag ctagaaacca tcatgaagtc catttcaata tgtgtatcaa tatgtttgac
     2521 caattcagcc ataaaaggaa tagatccttt taaaatccca actaagattg gattttttcc
     2581 tgcatagtct ttagttaatt gagcacctag ttttttagct gcttctgtaa tttcatcgtg
     2641 tgaaacgagg acttttttaa tatcgttttc taacattttt tacctatcta ttttttctat
     2701 ataaagtaca gtgttcatta tatcattttt cgttttttta ctcaaattat tggtcgcaat
     2761 tcccaaaatt gagacaattt caccaaattg ctcaataata agagcagagt ttctcttttc
     2821 catagggatt ttcaaatcaa taaataaacg tctgagtttt tttctatgcc cattttgaat
     2881 caaaacatct cctgtttttc gatgacgaat gtgtatggat gtttcacgtg aaacaggtat
     2941 ttgttgaatt gattcacctt ctaatggaag tccaaaggaa aataaatatc cttgataagc
     3001 tacctgattt tgatagtgta acacaagttc atcttcctct tcatcagcct gcggactgat
     3061 tttacaaatc tgaaactgtt ggtactcttt tatcaattca tagccatttt taatcggatg
     3121 acgatactgg cttttagatt ttaaaatctg ctgaacttca gcaaactgag cttttgtaag
     3181 attcaaatct ggaaaacgat tcagataagt ttgaagtaaa actctttgtg tagactcaga
     3241 gtaagaaaat aactgctgta aatcttccac attaatattg ttagataatt cagctattgc
     3301 caaatcataa tctaaaattt cattgccaat gcctaagatt gcatccctaa atcgaggatt
     3361 ttctttttcc aattctggta agtaagagtt tcgaatacga tttcgaaaat aatgattctc
     3421 ctgatttgat gtatcttcaa agtgaaaaat tgatggaaag tcttttttct gaaaatgcaa
     3481 gaagggacga atgatttcta tctctccgac tacttgcttc tccttaattc ctgatagata
     3541 gcgcaagcga gttcctcgaa tcaagcgcat caaaatcgtt tccacctgat catcagcatg
     3601 gtgggcagtg actaaagctg tcgcacctgt ctttttcatg acctcttgaa aaaaatcata
     3661 acgaaaattt cgtgcacgcg cttctgaaaa ttctcctgaa aaattgctga tataaatagg
     3721 aagctctgct tcagcagcca acttccttaa ttccttttct tcccaatctg attcaattct
     3781 ctgcttatga ttcacatgag ctagaatcaa ttcaatctct aactcttttt gataagtaga
     3841 caatacctta aatagaaaca tggaatctaa tccaccagaa agagctagaa ccgccttagc
     3901 atgctttttg aaatatccct tcttgagaaa atgatttaaa aaatctggtt ccctcatttt
     3961 agaacctcat aaacatcctt ggctatctta gaaatcgtat cataatcaga attcttagtg
     4021 aaaatagaaa gaataaatgg agaatctgca tagacaacac ccgtatcatg cttaaattca
     4081 tccgcatctc caattttatg agctacttta acagaaacac ctttggcaat tcgctgacta
     4141 tcaaaatctg ttttagtcaa agactctaac acaaatccat tttgattata aatagcttcc
     4201 ataaacttcc cggccatctt agaagaaatc aatttttctt ttggatccca atcatctccc
     4261 ataatggcag acatcttgga tttgaatgtg gcatcagatt ggtttgaaat gtaatatccc
     4321 aatagattat gagctacatt atcagattct tttgatactt tcgtaattaa atcctttaaa
     4381 gaatattctt tattatcttc ttttttagga agactaccac ttccctctgg tttataagaa
     4441 cctggaaaat cattgactgc agatacgtat tttacagtcg tatctaactg ataaagaccc
     4501 tcatttattt tttcttgcgt ataatagaga taagagagtt tcaaaacgct ggctgcatac
     4561 atcttttcat cttgattgat accagcttct tttccagtag tcagttgctt aacataaata
     4621 gagaaagaat ctttctgata tttttcagat aacatttctt gaactttact catccgatta
     4681 tcttcttcag aagttgattc tttagctacc catccagcct gatcaatatg tagaaattct
     4741 cttccttcta caaacatggt cttgtcgatt gatacttgcg aataagctga taaggatgat
     4801 ttcacttctt ttaaatcata aggactattg tacagtttaa agtcagattc taaccatgct
     4861 ttttttattg ttggagttac ctctgattga tcatataaaa atcgtttgtc cgcagctata
     4921 aattgatgat ttgatagctt aaatactgga attccttgtt tatttaagcg ccactcggtt
     4981 atttgaaaac ttgttttggg agtcaatttc ccagattcca ctactaaatc ttcattcgca
     5041 taaacaggaa cctctccata aaccatggga gaacttaatt tttctctaaa ataaatacca
     5101 aagtcagatt gtgaaaggta ataaatttct ttcgaagtat agacgacttc tttttctgtg
     5161 ctaacgactt ttgaaatggt caaaaaactt ggtagcaaca aaataattaa gaatttacgc
     5221 attcttcctt tccttttctt cttgtaaacg caataattga tttttatcag ccaattcttc
     5281 cagcttttca tctgacaaac tcaaaaattg ctctattacg tctaataaat tttccatttt
     5341 atcacctttg aagcaagtca ggaatcgtat aaactttttc cctcgactta gaataatagt
     5401 acttcgctcg tgtatatttg gcagcataat cttcatcttt caacttggta gcaaatgctg
     5461 tctccttatc cttttcatca ctcaaagttt gatactgagt ttgcaagtct gctaattgct
     5521 gacgtctttg gagtaattgc tgataactct gcgctaaatt aaaagttggc aagataaata
     5581 atagcataat caaaatcaat acccctccca taaaacgatt ccgtttttgt cgttctttca
     5641 tcaggtagcg acgacgttgg tattcatttt gaataaaaga attattcaat tgtacaatat
     5701 ttttagacat tttcttctac ccgtgtttca ctgataattt catacattcc tgctgcatct
     5761 tctttttttg tactatcttt catctctagt acttttacaa gcagcaactt attgccaaag
     5821 cgaatttcaa cttggtcatt aactttcaag tccgttgaac ttttggccaa gattccatta
     5881 accttgattc tacctttatc tgctacttcc tttgcgactg tacgacgctt gataattcgc
     5941 gatactttta aatatttatc taatctcatc cttattacct caatttatta ttgtaccatt
     6001 tttatctatt ttatagaaga aaaatatcaa atggaatttt cttccttaaa ctcttttatc
     6061 tctaataaac tttctccaaa aatcagcaga ccttctaaaa tttcataatc tttcttattt
     6121 tggacatcaa atacaagctc cattaatccc ttattctcag cgatgcctgc ttttaagttc
     6181 gttacggata aagctttaaa ataatcttga gctaaaaaca atcgttgagt gactttttca
     6241 aattgaattg taattttatt atcttttctt tccacacgtt gaacaaagac cttgtccaag
     6301 tatgatttaa ccaaaccaat ctctaaaaga taggctacta catctgggta ttctccaaaa
     6361 cggtctatca actcctcttg taactcttca taattgacac ggttgtcaat ttgacgaatt
     6421 ttcttgtaaa tttcaatctt atgtcgttga tcagaaatat aagtatcagg aagataggca
     6481 tcaatttgca aaatcaactc agcattccct ttggttcttg tgttagcgtt agcattaccg
     6541 tttcgtttag caatagcttc ctctaataac tgcgaataca attcaaaacc aacagaatca
     6601 atgaaaccag actgggattt tcctaaaaga tttcctgctc cacgaatcga aagatctcgc
     6661 attgcaatct taaagccaga gcccaattct gtaaatcctt taatcgcttc taatctcttt
     6721 tcagagactt cactgattga tttttctgga cgatacatga gataagcata agcaatacga
     6781 ttactacgac cgactcttcc tcttaactga tataaggttg acaagcccat atggtccgca
     6841 ttttcaataa ataaagtatt agcatttgga atgtccaccc ctgtctcaat aatagtagtc
     6901 gtcaccaaga tatcgtattg tccctcaata aagtctaata gagtattttc caactggact
     6961 tcactcattc gaccatgaac atatccaatc gaagcctccg gaatcaactc ctgtaattct
     7021 gaaaccttct gaacaattgt gtcaactttg ttgtaaagat aataaacttg acctccacgc
     7081 tccatttcac gcaagacagc atcacgaatg acactatcat tcttttccaa aacataggtc
     7141 tgaacaggat agcgattagt cggcggagtt tcaataacag ataaatctct gattcccagc
     7201 atagacatat ggagggtacg agggattggc gtagcggtca aggttaggac atccacttgt
     7261 ttcttcagtt ctttcaaagt ttccttatgc ttgacaccaa atcgctgttc ctcatcaata
     7321 atcatcaagc ccaaatcagc aaacacaaca tcttttgaca aaacacgatg tgttccaatc
     7381 aaaatatcga cttgaccgtt tttcaatttt tcaagtgttg cagtctgctc ttttttactt
     7441 ctaaagcgac tcaacacatc aatattaact gcaaaatttt ggaatcgttc cttaaaattc
     7501 gtatagtgct gttgcgctaa aaccgtcgtc ggaactagaa tgacaacctg tttgtgatca
     7561 ttgactgctt taaaggctgc acgcatagca acttcagtct ttccaaaacc aacatcccca
     7621 actaaaagtc gatccattgg ctgagaagcc tgcatatccc tcttgatttc ctcaatacta
     7681 cgaagttgat catccgtttc aacataaggg aaagcatcat caaaggcatc ttgatcatca
     7741 tcatcagctg agaaagcaaa acccttcaac tgactacgtt cagagtagag tttgattaaa
     7801 tcatcagcta tatcctctac ctggttctta accttttgct tggccttttt aaaatgaccg
     7861 tcatttaatt tattgagttt tggagcttta ccatcacttg aaatatattt ggacagtaga
     7921 tgaatctgtt ccacggggat agaaatttga tcaccatttt ggtattggac actgacataa
     7981 tcgcgatgaa ttcccttgat ttcaatggtt tcaattccta gatattgacc aatcccatgg
     8041 atatgatgga caacatagtc ccctttttca agttcattgt aatcttttaa tctctctgca
     8101 tttgaaacat gttgtcttcg aaaacgacgc tttaatttct tttgaaaaat ctcatgttca
     8161 gttatcaata aaatcttttc atctacaaaa tgaaaaccat gtctgagatt accctctatt
     8221 aaatttacag attctttaca gatacttgtc ttatctctag aatccaattt aatctgatat
     8281 tcctctaaca tatcctccaa tgttttactt cccattgaat tgctagactg cagaataatg
     8341 gtgtaatcca tttttttata tcgttcaatt tcttctttta gaaaagaaaa ctgattgaaa
     8401 aattcctgta taggatattg attgaattga taaattttgt caaatttgag atttcctaaa
     8461 cccttttgaa gattagagaa aaaggtcact ggactttgtt ttttatagat ttgttcaata
     8521 tcagaaaaat actgcatatc agaaaatgct ttactattct gtaattcttc tgtaaagtac
     8581 tgcgctaatt ctctttcaaa gacttcatac tgattcatca atttttgata atcatcaaag
     8641 aatattggag tatctttttc aatataatca aagacagtcc atgtcttatc atagcacaaa
     8701 gataaaaact tccgagaatc tgcatgactt tgtttttggt gaaaacttga aagaatttct
     8761 tctaggtatg atttcaaaat aggtgataaa gtttttgaaa tttgtttttc taaagctgac
     8821 tgtcctcgtt gataatcctt ttctctcaaa agcatatcac tagctggaaa gatagtgagt
     8881 tctgtcttat tttctttcga taattgtgtt tctacttcaa atgacctgat accatcaatt
     8941 tcatcaccaa aaaactcaat tcgacaaggt tctaactggg atatttcaaa aatatctaaa
     9001 atatctcctc gaagactaaa ttcgccctga gtttgtactt gagtaacttt ttgatagcca
     9061 ttttccttta actgatggat aaacgcgtgt tgagcatatt cttcaccaac tgagattttt
     9121 acaatactat ctttgaatgc attgggagac ggtaaaatca atcgacttgc tgcgatatta
     9181 caaactaaaa tccctttctt agatgaatca gtcaaaaaac gcaaggcttc aacccgtgaa
     9241 ataatttttt cctgtgaaga catcaaaaac tccaccatag gagcatcatc taccaaaaat
     9301 ggatagacga gttcctcacc caagatagaa ataagatcac taacaagtcc ttctgcttct
     9361 ccataagttg acgtcaataa cacaatccta tcttcttttt ctaaactgct tgcaattgca
     9421 agagccttag tagatgttga taaaccaagt attagttgtc ttttcttatc tgttaaattt
     9481 tgatgccatt ttttaatctg atcattttct gagaataaat ctaataaggt caccatttat
     9541 ccgttatacc tctgcattgt tttctcaaaa tttttctctt gtaaatagta ttttacagaa
     9601 tcgtcaactt tgtcaacaga ctgtaaaata ccgatataat catccctgtc aaacttactc
     9661 aaaacatgat gaacaactga cataccattt ttaggtcttc caattccaat cttaacacgg
     9721 ttaaagacct gagttcctat atgttgaata atagacttga taccattatg accacctgct
     9781 gagccttttg ctcttaaacg aattttccca acttccatgt caagatcatc gtaaatgata
     9841 agtaaatcgt caatatccaa accatagtaa gttaataaag catgaactgc ttttccactt
     9901 tcattcataa aggtcgttgg tttaaccaga taaatttttt ctccatttag gaaaaaggat
     9961 gctaggtcag cttgaaatat cttatcgtgt gtaaaagtga cattctgttt cttcgctagt
    10021 tgatcaatca acataaaacc aacattgtgt tttgtttcaa aatatttatc ccctggattt
    10081 cccaaaccta caagtaattt ggtcatttat ttttcctttc aaaagccaaa agggttggaa
    10141 tttttttcca acctaattga caccatttat taatttttag acattaaagc ggaattctat
    10201 gatgtcgcca tcttgaacga tatattcttt tccttcttca cgcaagcgtc cagcttcttt
    10261 tacggccttt tcagatccgt atttcactag atcttcatat gacatggtta ctgcacgaat
    10321 aaagcctttt tcaaagtctg agtggataat accagctgct tgaggagcct tcataccacg
    10381 tttgaaagtc caagcgcgaa cttctttttc accagctgtg aagtaagttc ccaatccaag
    10441 caagtggtaa gctgcacgcg tcaacttatc tacacctgat tctgtcaaac caatggcttc
    10501 aagaaactct tttttatctt catcatccaa ttcagaaatt tcttcctcag cacgcgcaga
    10561 aataacgact acttcagcat tttctgtcgc tgcaaattca cgaatttgtt tgacatagtc
    10621 gatagagtca ggttctgaaa ccacatcctc gtccacatta gctacataaa gaactggttt
    10681 agtcgtcaaa aggaaaagac ctttgacaac cttttgttcc tcatctgtaa attcaatggt
    10741 acgagctgat ttcccgtctt ctaggactgg tttaatcttt tgaagaacat tgaattctgc
    10801 tactgattct ttatcttttt gcgtacgtgc catcttttct acacgcgcat atcgtttgtt
    10861 cactgattct aagtcagcaa gaattaattc cagattaatt gtatcaatat ctgcaagtgg
    10921 atctacaaag gcgtcttcac gtccttgctc gcgcatcaca ttttcatcat caaaagcacg
    10981 aactacgtga acaatcgcat ctacttcacg aatattggcc aagaatttat tccctagccc
    11041 ttctcctttt gaagctcctt ttacaatccc tgcaatatcc gtaaattcaa atgttgtggg
    11101 aactgtcttt ttaggagtta tcatttcagt tagtttttgt aggcgttcat ctggaacttc
    11161 caccattcca acatttggat caatcgtcgc aaatgggtag tttgctgcct ctgctcctgc
    11221 ttttgtaatt gcattaaata gtgttgattt accaacgttt ggcaaaccaa cgatacctgc
    11281 tgtcaaagcc atatttctca ttctccgttt tcatttcaat ccctaatatt ataacacaaa
    11341 aagggataaa gtgctaaccc tctaactaag ggttctcagt caataatttt attcattttt
    11401 cgctcaaaat catatcgccc catcatgaca acatgctcac aattactgca ttttattttg
    11461 atatctgctc ctacacgtgt aatttcccaa cgattagcct ttttaccagt cgacttgatt
    11521 gtacaagcgt gtgatttttt catctcaaca aaatttccaa cttgatacat actactctcc
    11581 ttttcttttt cgattatatc ataaaagagg cgagccaggc tcaacctctt tcacttaatt
    11641 tgtacgaact ggtgtaatga gctgcatgaa gtcttcgtca gtatctgctg gcacaagagt
    11701 aaatggacga acagctgaga taaagctaat ggtcaccttt tcgctattta aagctttaag
    11761 agaatcaatc aagtaagttg ggttgaaact aatggtcaaa tcttcaccag taacctgatc
    11821 agtatcgatt tcttcgttta ctttaccaac ttctggagag tgaacatggg cgctaacaac
    11881 cccatcctta atttcaagtt tcacagtacc attttgagtc gcacttgata aaagacgggc
    11941 acgctccatt gactggcgta agtttaccac attaaaagta atagtagtgt taaagtctgt
    12001 tggaatcaag cgatctgtat caggatagtt tccttctagg agacgagtat agaagctaat
    12061 attttcgctt ctaaagagga tttggttatt ggcaaagaaa atctctacag tttcgatatc
    12121 atctgtaaat accgctgaaa attcgcgtag agaacggcta ggaattacga catcaaaatc
    12181 atcactattt ttttcaagag tcaatttttt ctggcttagg cgatgagagt ctgttgcaac
    12241 tgtttttaac tctttgtgtt gactcaatac gaagtggaca cctgttaaaa tcggacgact
    12301 ctcttgtgta cttgcagcaa aggctgtttc attaataatt ttcttgagta attttgtttc
    12361 aagtattaaa ggagtgcttg ctgaaatttc ttggattcgt ggatattgtt cgctatcttt
    12421 tccttttagg gtaatttctg atttgccact ggttaaaaca atttgatttt gttcaatttc
    12481 tttaaaatca agagttacat caggtaaact agatactaca ttgataaaga aagaagcttc
    12541 aagaaggatc gaacctaaag aagtaattaa caaaccagca tcttcatttt tttgagaaat
    12601 aaaattttca attgaaattt gaccatttga accaattaaa gtaatacctt cattggtcac
    12661 gtcaattttt actgttgata aaataggaat ggcattttta gaactaatag ctctcttagt
    12721 agtatttaat gcttgtagaa ataaattttt attaattgaa aaatgaatca tggattctcc
    12781 tttatttatt tttagtatta gaaagataat agtaataata gagtctgtga aatctgtgga
    12841 aaacagcctt taaacaagtc atatcaagtt ttttagcttg tttaaaaaat gtggataaaa
    12901 aagataaaaa actaaaagtt atccacaaat tatttgattt tctttttgat tgattcaatt
    12961 tctaaacgta aattatcgtc ttgatcaatc aaagatttta ttttggcatg ggcatgaatg
    13021 actgtggtat gatcttttcc cccaaattcc ttcccaattt ttggaagact attatctgtt
    13081 agttctctag ataaatacat ggctacttga cgggccaaaa caatattttg aaggcgtcta
    13141 cttcccttca tttctttgat actaacacca taaaagttac caacttcagt ttggatttta
    13201 tcaattggga tgacgagcat ttggctaaca tcttgtttgc gggctctaat ggcttctgca
    13261 gcaatatcaa tagtgatatc cttgattttt tttactctgg caattaaagt gatgtcgttg
    13321 atggctccct caagatctcg aacatttgaa tcaaattgcc cagctaggta ttctagagta
    13381 tcactttgga aattgtagcc taaatgttcc gttttacttt gtaaaatggc aatacgtgtt
    13441 tcaaagtcag ggggtgtgat agtttgtgtc aatccccaac taaaacgcgt gacaagcctc
    13501 tcctcgagcc cttctagatg ttttggacta cgatcactcg ttaggacaat ctgtttttgc
    13561 ttgtcatgaa gggcgttaaa ggtattgaaa aattcttcct gagttgcgac tttttttccg
    13621 ctgagtgact ggatatcatc gattaacaaa agatcaagac tacgataggt ctttttaaac
    13681 ttttccattt ccccaagtct taggtgatca agaaagtcat taataaagct ttcggcaggg
    13741 atatatttaa cacgcgcatt aggaatattt tttagaattt catttccaat agcgtttaat
    13801 aagtgagtct taccaaggcc tggtcctcca tagataaaaa gagggttata ggtcagagcc
    13861 aaatcttcag agacagctaa agcggctgac acagcccaaa catttccatc cccttgaata
    13921 aagttatcaa aggtatactt ttcttttaat cccgtatctg aataaggaat agatactaac
    13981 tttggactat agttataaag agttaaattt gtagcttctt caacttgtga gctagtcgta
    14041 tcttgaggtt tggtgaaaat atagtgggga gttatttcag cgtcataaat ttcaaaacca
    14101 gctactacaa taatatcttt tagttgtttt tcccagacca tttccatttc agagcgaggt
    14161 agaaatatag tggcaacatt ttcctctacc ttgatgagtt cagcttgaat agcatagaaa
    14221 tcatacatgg atcgagtcag tctttcttgt gcaaattcta atatacgatt ccaaaattgt
    14281 ttttctttca atcctttctc ctcctttact atttaaaagt ttaatactag agatatttta
    14341 ccatagatag caagaatttt ccacaagctg tgaaaaataa tccacaatgt taccaaactt
    14401 tatccacagg ttggggataa aagaagaaat tattgattta ttaagctttt tattgaaaaa
    14461 catagtggat aaccttgtga gataaaaaaa taaaagaaca gccttatttc aggctgttga
    14521 taattctact atattcttct tgatttgaaa aagaaataat gatttttcca ctgtcttttt
    14581 tagatagttt aatttctaca tctaatccga gtagttttct taactgtttt tcttcatttt
    14641 gtatgaaatg attagttttt tgctgtttct tttgtttttt ctctgtcaga agagcttcta
    14701 atttccttac agaaatatct tcttctataa tccgttgaaa gaaatagtct tgttgttcct
    14761 tatttaaccc aactagggaa cgcgcatggg cttgtgatag tttgccattt tctacttctg
    14821 aaagaatctg ttctggcaag gaaagtaaac gaatggagtt gctgatatat ggacgagact
    14881 tgcccatctt atctgcaatt tcagcatggg tgaatccttt ctctacgaga gattcatagg
    14941 cgcgtgcttc ttctattggg tttaaatttt ctctctgtaa attttcaata atggactgga
    15001 ccatcatctc ttggtctgaa atctgtttaa caacagctgg gatagaccgt agaccagcta
    15061 aaagtgaagc ccgatagcgt ctctctcctg caaggatttc ataaccaata acaggagatt
    15121 gacgaacaat aatcggttga atgaccccat tttctttgat agactgtgct agttcatcta
    15181 gtttttctct atcaaattct tttcggggtt gatagggatt tttttgtata tctgtgatag
    15241 aaatcatttc aaatttttcc atgattctac actaacacat cttttctctt atgtaaagct
    15301 ttctttacat agatgtcaat taagattcta aatcacctga actcttgtta agtttgatag
    15361 aggtagtttc ttctttcccg ttacgatagt aggttatctt aatggtgtct ccgatagaat
    15421 ggttgtaaag agcactttgt aagtctgttg atgaagcaat ctctttgtca tctacttttg
    15481 taattacatc gtatttttca aggtgaccat tggcaggcat attactttgt accgaacgaa
    15541 caactacacc agatgtaaca ttacttggaa tattgagtct tctgatgtcg cttgtactca
    15601 cattagataa attaaccatc tggattccca aagctggacg cgtcactttt ccgttttttt
    15661 ctaactgttc aataatattg atagcatcat ttgcaggaat tgcgaaacca agaccttcta
    15721 cagatgttcc tccatttgta gcaattttac ttgaggtaat tccgataacc tgcccttgaa
    15781 tattgatcag tgggccgcca gagttacctg ggttaatagc agtatcagtt tggatggctt
    15841 ttgtagaaat agcttgtcca tcttccgatt ttaaggatac atttctattg agactggata
    15901 cgataccttg agtgacagta tttgcatatt cagaacctaa cgggctaccg atggcaatag
    15961 cagtttctcc tacagttaac ttactagaat caccaaactc agctactgtt gtcacttttt
    16021 ctgaagagat ttttacgaca gcaatatcag agaaagtgtc agctccgaca atttctccag
    16081 gtactttagt cccatctgac aatcgaatat ctactttgct ggcgccattt ataacgtgat
    16141 tgttggtgac gatgtaagct tctttatcat tctttttata aataactcca gatccttcac
    16201 tagagattcg ctgagaatct gtgtcagtat catcattgcc aaatacgcta ttttgtctgt
    16261 ttgccgaata agtaataaca gaaacaacag catcttttac tttgttaaca gcctgtgttg
    16321 ttgaattttc gttcttatag gcagtttgtg taatagtact attgttgtta gagttgttta
    16381 cactactttt ttgagttagt tgagttattg aaaaactacc caaggctcca ctaaaaaagc
    16441 taatgacgat aacgactaat aattgaaacc attttttgta aaatgttttt agatgtttca
    16501 tatttgcctc catatgtttg aattactgaa agtataaact gactagctta attataactt
    16561 aaacacaaaa gttttacaca aactgtggat aactcttttg aaactgtgat tttcttaatt
    16621 gaaatctatt ttttattttg tgaataagat gtgaaaaaat agagaatatg ttagaataga
    16681 gtcatgaaaa ttaaagttgt aacagttggg aaactgaaag aaaagtattt aaaagatggt
    16741 atcgcagagt attcaaaacg aatttctaga tttgctaagt ttgaaatgat tgagttatca
    16801 gatgaaaaaa caccagataa ggccagtgaa tcagaaaatc aaaagatttt agaaatagaa
    16861 ggtcagagaa ttttatcaaa aattgctgac cgtgatttcg ttattgtgtt agccattgaa
    16921 gggaaaactt tcttctcaga agaatttagt aagcagttag aagaaacttc tataaaagga
    16981 ttttctactc ttacttttat tattggggga agtttaggat tgtcatcatc tgtaaaaaat
    17041 agagccaatc tttctgtcag ttttggtcgc ctaaccttac cccatcagtt aatgagacta
    17101 gttcttgttg aacaaatcta tcgcgctttt acgattcagc agggattccc ctaccataaa
    17161 tagaaaattg acttttaatt gaatttttgg tagaataatt gtattaggtc tcatagctca
    17221 gctggataga gcattcgcct tctaagcgaa cggtcgcagg ttcgaatcct gctgggatca
    17281 atataatagc aaagctggga attttcccgg cttttttctt aaaaaagtac actttgggag
    17341 aaaaaaatga cagttgagag aattttatct aaaacgaaat tccattttgt ataatggttt
    17401 ttgtaagtta gcttacaaga aaaaacattt taggagattt tattatgaaa aacacagtta
    17461 aattggaaca gtttgtagct ttgaaggaaa aagacttaca aaagattaaa ggtggggaga
    17521 tgaggttgtc aaaattcttc cgtgatttta ttttacaaag aaaaaagtaa tgaaataagg
    17581 ggaaagagta atggatttat ttggatttgg gacggttatt gttcattttt taattattag
    17641 tcacagttac cattttattt gtaaaggtca aataaataga aaagaattat tcgtttttgg
    17701 tgcttataca ttactaacag aaatagtatt tgattttccc ttatatattc tatatttaga
    17761 tgggttaggg attgaaagat ttttatttcc tttgggatta tattcctatt ttcgatggat
    17821 gaaacagtat gagagggata gaggactatt cctaagttta ctactatctc ttttatatga
    17881 gagcactcat aactttctgt ccgtaacttt ctcctctata acaggagata attttgtttt
    17941 acaatatcat ttcccattct ttttcgttgt gacggtgtta acctattttg ttacattaaa
    18001 aatcatttac tatttccatt tggaactagc ctattttgac gaggactacc tttatccttt
    18061 cttgaaaaaa gtattttttg ctttactatt gctacatatt gtatctttcg tttcagatat
    18121 ggtaagtacg attaaacatt tgaatagttt tggaagtatt ttgtcatcta ttgtctttat
    18181 ctctctcctt ttgaccttct ttgcaatgaa ttctcataaa gttcaaatgg agaaagagat
    18241 tgctttgaag cagaagaaat ttgaacagaa acatttacag aattacacag atgaaattgt
    18301 tggtctgtat aatgaaatcc gtggttttcg acatgattat gctggaatgc ttgtcagcat
    18361 gcagatggca attgacagtg gtaatttaca ggaaattgac agaatttaca atgaagtttt
    18421 agtcaaagca aatcataaat tgcgttcaga taagtacact tactttgatt tgaacaacat
    18481 agaagactca gctttacgaa gtttggttgc tcagtcaatt gtctatgctc gaaataatgg
    18541 tgtagagttt acactggaag taaaagatac gattaccaag cttccaattg aactattgga
    18601 tttggttcgt atcatgagcg ttttattgaa taatgctgtc gaaggatcgg ctgatagcta
    18661 taaaaagcag atggaagtag cagttattaa gatggaaact gaaacagtta ttgtgattca
    18721 gaattcatgt aaaatgacga tgactccttc aggagatcta tttgccttag gattctccac
    18781 taagggaaga aatcgcggag tcggattaaa taatgtgaaa gaactactag ataagtacaa
    18841 caatattatt ttagaaacag agatggaagg cagtacattt agacaaatca ttagatttaa
    18901 gagggaattt gaatgaaagt tttaatttta gaagatgtta ttgaacatca agtgagacta
    18961 gagagaatat tggatgaaat ttcgaaagaa tcgaatattc caatatcata caagacaacg
    19021 ggaaaagtcc gtgaatttga agaatacatt gaaaatgatg aagtaaatca gctttatttc
    19081 ctagatatcg atattcatgg aattgagaaa aagggatttg aagtggctca gctcattcgt
    19141 cattacaatc cttacgctat tatcgtcttt atcactagtc gatcagagtt tgcgactcta
    19201 acctataaat accaggtatc agccctagat tttgttgata aggatatcaa tgatgagatg
    19261 tttaagaaga gaattgagca aaatatcttc tacacgaaga gtatgttact tgaaaatgaa
    19321 gatgttgtag attatttcga ctacaattac aagggaaatg atttaaaaat tccttaccat
    19381 gatattttgt atattgaaac aacaggggtc tctcataaat tgcgcattat tggtaagaat
    19441 tttgcaaaag agttttatgg taccatgaca gatattcagg aaaaggacaa acatactcag
    19501 cgattttatt ctcctcacaa gtcatttttg gtaaatatag gcaatatcag agaaattgat
    19561 cgaaaaaact tagaaattgt tttctatgaa gaccatcgtt gtcctatttc aagattaaaa
    19621 attagaaaat taaaagatat tttagagaaa aaatctcaaa agtgattgac aattagcaag
    19681 aaattgatat aatggttata tctgctacag atagaaggtc cgttggtcaa ggggttaaga
    19741 caccgccttt tcacggcggt aacacgggtt cgaatcccgt acggactatt ttatccgcca
    19801 tagctcagtt ggtagtagcg catgactgtt aatcatgatg tcgtaggttc gagtcctact
    19861 ggcggagtat agataaaagg gaacacagct gtgttcctct ttttgtatca atttgtatca
    19921 ccaagcattt tcataaggaa gtctgttatt tcttgagaac tttctttttt tccatgtgca
    19981 atccaagttt ggcagacacc aaaaagtgca tgagttagat agatgctact atattctaat
    20041 tcggtggtat ttagattcag ttgcataaat cgcttttgta aatctgtact aagcatgata
    20101 tgaagtttat ttcgtaagaa attttggatt tctttagtcc cattttcaga aagaagggca
    20161 gccagaagtg gttctgactc tagatattca aaaacttcta aaatagcgtc tcttttgtga
    20221 tgagcatgtt tttgaaaaat atattcaaat gtatggaata gcttgctttg atagtgctca
    20281 atcatatcat acttatcctt atagtgagta tagaagctgg aacgactaat tccggctttt
    20341 tctactaatt tgacagtaga aattttatca aatggctgtt ccatcagtaa ttgtaccata
    20401 gcattttcaa tagttcgctt tgtttttaag cgtttgttac tttcttgcat atttcctcct
    20461 tgtaaacaaa ttagactata tgtttaaaaa tagatttttt atcttgtaat ttagattttt
    20521 taatgtataa tctattatat caaaatttta gacaatatgt ttaaaaaagg agaaactaag
    20581 tttaaagaat ggaaagcaat ttaaaaaaaa ccaaccttta ttattgtcat gatcgggatt
    20641 tctcttattc cagatctgta caatatcata tttttgtcat caatgtggga tccatatggg
    20701 caattgtctg acttacctgt ggcagttgta aataatgata aagaggcttc ctataatggt
    20761 aatactatgg caataggaaa agacatggtg tccaatttaa aagaaaataa aaccttggat
    20821 tttcattttg tagatgaaga ggaaggaaag aagggattgg aagatggcga ttactatatg
    20881 gtagtgactt taccaagtga tttatctgaa aaaacaacta cattatccaa tattcaatcg
    20941 acagcagctt atcaatcatt gacaagtgag caacaaactg agataagtga ttctgtatct
    21001 caaaattcaa ctgatagtat tcaatcggct cagtcaattg tagctttagt acaagattta
    21061 cagggaagtt tagaaaactt acaaaatcaa tcttctaatc tttcgacttt aaaaaatcaa
    21121 tctaatcaag tatcacctat tacttctact tctttgatag gattgtcaag tggattaaca
    21181 gagatacaag gagatgttac tagcaaatta gttcctgcca gtcagtcgat tgcatcaggt
    21241 gtaaacgcat atactacagg tgttgataaa gtttctcagg gcgcaagtca actaagtgaa
    21301 aaaaatgcca ccttgacagg tagtttggat aaactagttt caggctcaaa caccttgaca
    21361 caaaaatctt ctagattgac agcaggagtt ggttaattac aatcaggatc tgggcaatta
    21421 gcagacaaat ccagtcagtt actttcaggt gcttctccat tagagaatag agctaataaa
    21481 ttggcagatg gatctgggaa actagcagaa ggtggaacaa agttaacttc tggattggaa
    21541 gatttacaga caggacttgc ttctttagga caaggactag gtaatgctag tgatcaactc
    21601 aaatcagtat caacagaatc taaaaatgca gagattttgt caaatccact caatctttca
    21661 aaaacagaca atgatcaagt tcctgtaaat ggaatcgcaa tagctcctta tatgatatca
    21721 gttgctcttt ttgttgcagc aatatcaaca aatatgatat ttgcgaaatt gccttcagga
    21781 cgtcatccag agagccgttg ggcttggttg aaatctcgag ctgaaataaa tggtattata
    21841 gctgttttgg caggaatttt ggtatatgga ggagttcagc ttattggttt aactgctaat
    21901 catgagatga gaatatttat tctcatcatc ctaacaagtt tagtattcat gtctatggtg
    21961 accactttaa caacgtggaa tagccgtata ggagcttttt tcttacttat tttgctttta
    22021 ctacagttag catcaagtgc aggtacttat ccacttgctt tgacaaatga tttctttaga
    22081 tctattaatc cctggttacc aatgagctat tcagtttcgg gattacgaca aacaatctct
    22141 atcaacaagt cattttccta gctgtcatac tagctctatt tactggttta ggtatgctag
    22201 cctatcaaca taagaaaatg gaagaagatt aaaaaaatcg accgattaat gaactgcacc
    22261 ccaaaagtta gacagaaaaa atctaacttt tgaggtgttt tattatgaaa ttaa
//
LOCUS       DAVBCB010000036        21460 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVBCB010000036 DAVBCB010000000
VERSION     DAVBCB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.036"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            66..287
                     /locus_tag="VQU28_001334"
     CDS             66..287
                     /locus_tag="VQU28_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476685.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            804..1043
                     /locus_tag="VQU28_001335"
                     /pseudo
     CDS             804..1043
                     /locus_tag="VQU28_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            1187..2416
                     /locus_tag="VQU28_001336"
     CDS             1187..2416
                     /locus_tag="VQU28_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HET2476686.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            2436..5132
                     /gene="ppc"
                     /locus_tag="VQU28_001337"
     CDS             2436..5132
                     /gene="ppc"
                     /locus_tag="VQU28_001337"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HET2476687.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATNLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYEIILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    5215..5432
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5545..6549
                     /locus_tag="VQU28_001338"
     CDS             5545..6549
                     /locus_tag="VQU28_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2476688.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTIKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            6618..7484
                     /locus_tag="VQU28_001339"
     CDS             6618..7484
                     /locus_tag="VQU28_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476689.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            7481..8239
                     /locus_tag="VQU28_001340"
     CDS             7481..8239
                     /locus_tag="VQU28_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476690.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            8329..10089
                     /gene="dnaG"
                     /locus_tag="VQU28_001341"
     CDS             8329..10089
                     /gene="dnaG"
                     /locus_tag="VQU28_001341"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HET2476691.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVTFMEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAAILKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMERISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            10092..11201
                     /gene="rpoD"
                     /locus_tag="VQU28_001342"
     CDS             10092..11201
                     /gene="rpoD"
                     /locus_tag="VQU28_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HET2476692.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            11215..11544
                     /locus_tag="VQU28_001343"
     CDS             11215..11544
                     /locus_tag="VQU28_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2476693.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            11659..12702
                     /gene="cpoA"
                     /locus_tag="VQU28_001344"
     CDS             11659..12702
                     /gene="cpoA"
                     /locus_tag="VQU28_001344"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HET2476694.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            12718..14043
                     /locus_tag="VQU28_001345"
     CDS             12718..14043
                     /locus_tag="VQU28_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2476695.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGILIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGALYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFRTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            14074..14250
                     /locus_tag="VQU28_001346"
     CDS             14074..14250
                     /locus_tag="VQU28_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HET2476696.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            14309..14443
                     /locus_tag="VQU28_001347"
     CDS             14309..14443
                     /locus_tag="VQU28_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HET2476697.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            14507..15817
                     /gene="obgE"
                     /locus_tag="VQU28_001348"
     CDS             14507..15817
                     /gene="obgE"
                     /locus_tag="VQU28_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HET2476698.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            15827..15985
                     /locus_tag="VQU28_001349"
     CDS             15827..15985
                     /locus_tag="VQU28_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476699.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            17035..18294
                     /locus_tag="VQU28_001350"
     CDS             17035..18294
                     /locus_tag="VQU28_001350"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HET2476700.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            18310..18861
                     /locus_tag="VQU28_001351"
     CDS             18310..18861
                     /locus_tag="VQU28_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476701.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLFIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            18854..20131
                     /locus_tag="VQU28_001352"
     CDS             18854..20131
                     /locus_tag="VQU28_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2476702.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIDRLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            20147..21007
                     /locus_tag="VQU28_001353"
     CDS             20147..21007
                     /locus_tag="VQU28_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HET2476703.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            21230..21340
                     /locus_tag="VQU28_001354"
                     /pseudo
     CDS             21230..21340
                     /locus_tag="VQU28_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818216.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase"
BASE COUNT         6560 a         3687 c         4816 g         6397 t
ORIGIN      
        1 ccagttagga cactttgaat gatttcttag ttttatatat tatacttttc tcaggagaaa
       61 gctagatgta cagacgtttg agagatttga gggaagatca cgatttgacc caaaaacaaa
      121 tagctaaaat actttcgttt acaaactcag cttatgctaa aattgaacgg ggtgagcata
      181 cgttaacagc agatgtattg gtaactctct caaactttta cgatgtcagt acagactacc
      241 tattggaatt gacagatttt cctgataaaa ttcgctttag aaaataatct cctcaatttc
      301 atagagtgtg aaaatgagga gattttttat ttgttctttg aaaattaaat agtccaaaat
      361 ggtactttcc tcatttgtgg agcaaatttg aatggctcgc catgataaga gcgattttaa
      421 agccatcaat aaaatagagc gatactttat atgccatgat acaaatgata tacaatgata
      481 cttctgaccg ttcaggctgc catcgtaaaa gagcagcaag tgaaattctt atgatgactt
      541 catcagtcat gccacgttga atgtgtgagt ttgttagaga aacgcaatta atcctcaaaa
      601 ggttccccga accttttgag ttccacagac gcatcacgtg gagtgtgtaa gcttgttgct
      661 aaaagcgtaa aaaccttgga acgaaaggaa taatagactt cctgcgaaac aaaaatacaa
      721 tacaataaaa ctatggatga tgaagcaagt aaacaattaa gcgatagccg tttcaagagc
      781 cttgtaggtg ttcagcgcac gagatgttag ctgtgttaaa aacagcttat caacgcaacg
      841 taaacgcgca aaaggtggac gaaaaaccaa attaagccta gacgatctcc tcatggtaac
      901 tattcaatac atgcgagaat agcgcactta tgaacaaatt gcggctgatt ttggcattca
      961 cgaaagcaac ataattagtc ggaggtcaat ggattgaagc aactcttatt caaaatggtt
     1021 ttacgatttc aaattctgcc ttaattctgt aaaaacagta aaattcgaag gattgtaagg
     1081 taagagtttt tttctttctg aaaaaatggt ataatagcaa tcaaaactag aaaataaaac
     1141 ggaatttgga acagatttgt ctgtatccta gtagagtggt gatactatga agattagtaa
     1201 gaggcactta ttaaattatt ccatcttgat tccctacttg cttttatcta ttttgggctt
     1261 gattgtggtc tattcgacca ccagtgctat tttaattgaa gaaggcaaga gcgccttgca
     1321 gttggttcga aaccaaggaa tcttttggat tgttagtttg atactgattg ccttaattta
     1381 taaattgaga ctagattttt tgagaaatga gcgactaatc attttagtta tattaataga
     1441 aatgctttta ttgttcttgg ctcgttttat tggtatttca gtaaacgggg catacggttg
     1501 gatttcggtt gcaggagtaa ctattcagcc agctgagtac ttaaaaatca ttattatttg
     1561 gtatttagct caccgattct ccaaacagca agaagaaata gctacttatg attttcaagt
     1621 tttgactcaa aatcaatggc ttccccgtgc ttttaatgat tggcgattcg ttctcctagt
     1681 tctgattgga agtttgggaa ttttccctga tttaggaaat gcgactattt tagtcttggt
     1741 ttccttgatt atgtatacag ttagtggaat cgcttatcgc tggttttcaa ccattctggc
     1801 gctcgtatct gccgcttctg tctttgtctt gaccactatc agcctaatcg gtgttgagac
     1861 cttttcaaaa attccagtat ttggctatgt agccaaacgc tttagtgcct tttttaatcc
     1921 ttttgccgat cgtgctgatg caggtcacca gttagctaat tcttattttg ccatggtcaa
     1981 tggtggttgg tttggtctag gtcttggaaa ctcgattgaa aaacgaggtt atttgccaga
     2041 agctcataca gactttgtct tttctatcgt gattgaagaa tttggctttg ttggtgccag
     2101 tcttatttta gctctcttgt ttttcatgat tttgcggatt atcttggtcg gtattcgagc
     2161 ggagaatcct ttcaatgcca tggttgcact cggtgtcgga gggatgatgt tggttcaggt
     2221 atttgtcaat atcggaggga tttcgggctt gattccatct acaggagtaa ccttcccctt
     2281 cttatcccag ggtggaaata gtcttctagt cttatcagtg gcagtagcct ttgtcttaaa
     2341 tattgatgcc agtgaaaaac gcgctaagtt gtaccgagaa ttggaaaatc aaccaatgaa
     2401 ccttctgttg aagtaggata aagaaaggat agtttatgtc tcttcaaaaa ttagaaaatt
     2461 ctagtaataa aagtgttgtg caagaagaag tcttgattct aacagaatta ctggaagata
     2521 ttactaaaaa tatgcttgcc ccagagacct ttgaaaaaat aatacagttg aaagaattat
     2581 caacgcagga agattatcaa ggtctaaacc gtctagtgac tagcttatca aatgatgaaa
     2641 tggtctatat ttcacgctat ttctctatct tgcctctttt gattaatatt tcagaggatg
     2701 tggatttagc ttatgaaatc aatcatcaaa ataatattga tcaggactat ttaggtaaat
     2761 tatctacaac gattaaattg gtagcagaaa aggaaaatgc cgttgagatc ctagaacact
     2821 tgaatgttgt tccggttttg acagcccatc caacacaagt gcaacgcaaa agtatgttgg
     2881 atttaacaaa tcatattcat agtcttttgc gtaaataccg tgatgttaag ttggggttga
     2941 tcaataaaga taaatggtac aatgatttgc gtcgttacat cgaaattatc atgcagacag
     3001 acatgattcg tgagaaaaaa ttaaaagtga ctaacgaaat cacgaatgct atggaatatt
     3061 ataacagctc ctttttgaaa gctgtacctc atttgacgac ggagtataag cgcttagcgc
     3121 aagcgcatgg tctgaattta aaacaggcta aaccaatcac catgggtatg tggataggtg
     3181 gtgaccgtga tggaaatcca tttgttacag caaagacctt gaagcagtct gcactcactc
     3241 aatgtgaagt catcatgaac tactatgata aaaagattta ccaactttat cgtgaatttt
     3301 ctctttcaac tagcattgtc aacgtcagca agcaagtcag agaaatggct cgtcaatcca
     3361 aggataactc gatttaccgc gaaaaagagc tttaccgtcg tgccttgttt gatattcaat
     3421 caaaaattca ggcaactaaa acctatctga ttgaggatga agaagttggg actcgttatg
     3481 aaaccgccaa tgatttctac aaggatttga ttgccattcg agattctcta ctagagaata
     3541 agggcgagtc cttgatttca ggtgattttg tggaattatt gcaggcagta gagatatttg
     3601 gtttttacct agcatcaatt gatatgcgac aagactctag cgtctatgaa gcctgtgtgg
     3661 cagaactctt gaaatcagca ggaattcatt ctcgttatag cgagttgagc gaagaagaaa
     3721 agtgtgacct tcttttgaaa gaattagaag aagatccccg aattctttct gcgactcacg
     3781 cagaaaaatc agaattatta gcaaaagaac tagctatttt taagacggct cgtgttttga
     3841 aagataagtt gggagatgat gtcatccgtc agaccatcat ttcacatgca accaaccttt
     3901 ctgatatgct agaattagct attctgttaa aagaagtagg actggtggat acggaaaggg
     3961 cgcgtgttca gattgttccc ctttttgaaa caattgaaga cttggatcat tcagaggaaa
     4021 caatgagaaa atatctttct cttagccttg ccaaaaaatg gattgactca cgaaataact
     4081 accaagaaat catgcttggc tactctgaca gtaataaaga tggcggttac ttgtcatcat
     4141 gttggaccct ctacaaggct caacaacaat tgactgctat tggagatgaa tttggcgtta
     4201 aggttacctt cttccatggt cgtggtggta ctgtcggtcg tggtggtggg ccaacctatg
     4261 aagccattac atctcaaccg ctcaagtcta tcaaggatcg tatccgtttg acggagcagg
     4321 gtgaagtaat tgggaataaa tacggtaaca aagacgccgc ttactataac cttgaaatgc
     4381 tagtatcggc agctattaac cgtatgatta ctcagaagaa gagcgatacc aataccccaa
     4441 atcgttatga agccattatg gatcaagtag tggaccgtag ttacgatatc taccgtgatt
     4501 tggtctttgg taatgagcat ttctatgatt atttcttcga gtcaagtcca atcaaggcta
     4561 tttcaagttt taatattggt tctcgtccag ccgctcgtaa gactattact gaaatcggtg
     4621 gtttgcgtgc catcccttgg gtattctcat ggtcacagag tcgtgttatg ttccctggat
     4681 ggtacggggt tggttcaagc ttcaaggaat ttatcaataa aaatccagag aatattgcta
     4741 tcttacgaga tatgtaccaa aattggcctt tcttccaatc gcttctttca aatgttgata
     4801 tggttttgtc aaaatcaaat atgaatattg cttttgaata tgctaaactt tgtgaagacg
     4861 agcaagttaa ggccatctat gagattattt taaatgaatg gcaagttact aagaacgtta
     4921 tcttggctat tgaaggacat gacgaactct tagctgacaa tccatatcta aaagctagtc
     4981 tggattaccg tatgccttac tttaatattc tcaactatat tcagttagag ttgattaaac
     5041 gccaacgtcg tggagaattg tccagtgatc aagaacgatt gattcatatc accatcaacg
     5101 gaattgcgac aggattgcgt aattcaggtt gataattttc aagagtgaat gctaaaagtg
     5161 aatatcaaaa aaattctaat agactattga caagtagttt aaaaatgata taatttaacc
     5221 attcagaaaa gtaatcatac aaacttttta gagagtctgt ggtagctgaa aacagataag
     5281 tggcgatgat gaaaattggg ctgaatgcta tttagaattt gaaattataa aaattcggta
     5341 agcacacctt acagtgcatc tcgttattgc gagactgagc gatagggaaa ttccctataa
     5401 ttgaggtggt accgcgcatc gacgtcctca cacaagtttt ttgtgtgagg atttttttga
     5461 tggaggttag tatggaaaga aaacgatggc gtcgcttgtt tagataagtg aaatatgtta
     5521 aaggaaataa aaaggagaaa cagaatgaaa aataaacgtt taattggaat tattgctgca
     5581 ttagcagtct tagtagcagg aagcttgatt tattcttcaa tgaataaatc agaagctcag
     5641 aataataagg atgagaagaa aataaccaag attggtgtgc ttcaatttgt gagccatcca
     5701 tcccttgatt tgatttataa agggatccaa gatggacttg cagaagaagg atataaagat
     5761 gatcaagtta aaattgattt tatgaactca gaaggtgatc aaagtaaggt tgcgacaatg
     5821 agtaaacaat tggttgcaaa tgggaatgac cttgtggttg gtattgcaac accagcagcc
     5881 caagggttgg ctagtgcaac aaaagaccta ccagttatca tggccgctat tacagaccca
     5941 attggtgcta acttggttaa agatttgaaa aaaccaggtg gtaacgttac aggggtatct
     6001 gaccacaatc cagctcaaca acaaattgaa ctcatcaagg ctctgacacc gaatgtgaaa
     6061 acaatcggag ctctttactc aagtagcgaa gacaattcaa aaacacaggt tgaagaattt
     6121 aaggcttatg ctgaaaaagc aggtctgaca gtggaaacat ttgcagttcc ttcaacaaat
     6181 gaaattgcct caactgtcac tgttatgact atcaaggtag atgctatttg ggttccaatt
     6241 gataacacca ttgcatcagg atttccaacg gttgtctcta gcaatcaaag ttctaagaaa
     6301 ccaatttatc ccagtgcgac agctatggta gaagtaggtg gtttggcatc agttgtaatt
     6361 gaccaacatg accttggtgt ggcaacaggt aaaatgattg tgcaagtctt gaaaggtgca
     6421 aaaccagccg ataccccagt caatgtcttt tcaactggta agtcagtcat caataaaaaa
     6481 atagcacaag aactaggtat tactattcct gagtctgttc tcaaagaagc aggacaagtc
     6541 atcgaataaa taaggtaggg aggaggagac tcttcccatt ttttaaaaag atgaatatag
     6601 aaaggattaa ataaaatatg attgtttcca ttatttctca aggatttgtc tgggctattc
     6661 taggtctggg aatctttatg acatttagga ttttaaactt tccagatatg acgacagaag
     6721 gttccttccc tcttggggga gctgttgctg tcactttgat aaccaaaggc gtgaacccat
     6781 ttttagcgac acttgttgct gtaggagcag gttgtttggc tggaatggca gcaggccttc
     6841 tttatacaaa agggaagatc ccaaccttgc tctcagggat tttggtgatg acttcttgtc
     6901 actcaatcat gctcttgatt atgggacgtg cgaatttagg cctgcttgga accaagcaaa
     6961 ttcaggatgt tttgcctttt gattcggatt tgaatcaact cttgacaggt ctcatctttg
     7021 tgagcattgt tattgctctc atgctctttt tcttggacac taaactcgga caagcctata
     7081 ttgctacagg ggataatcct gatatggcta gaagtttcgg gattcatact ggacgcatgg
     7141 agctcatggg cttggtctta tcaaatggtg tgattgccct tgcaggtgcc ctcattgctc
     7201 agcaagaagg ctatgccgat gtgtctcgag ggatcggggt tatcgttgtg gggcttgcaa
     7261 gtttgattat tggagaagtt attttcaaga gtttgagctt ggcagagcgt ttggttacta
     7321 tcgttgtagg ttctatcgct tatcaatttt tagtgtgggc agttatcgca cttggcttta
     7381 atacaagtta ccttcgttta tacagtgcct tgattttagc agtctgcctc atgattccaa
     7441 catttaagca aacaatcttg aaaggagcca agttaagcaa atgacagcaa ttgtagaatt
     7501 aaaaaatgca accaaaattg ttaaaaatgg ctttgatgaa gaaaagatta ttttaaatga
     7561 tgtttcctta gaaatttttg aacgggactt tatcacgatt ttgggcggaa atggtgctgg
     7621 aaaatcaacc ctctttaaca ctatagcagg gaccttatca ctaactagtg gaactatccg
     7681 tattttaggt gaagatctca ctaagttttc acccgagaag cgtgccaagt acctgtctcg
     7741 tgtcttccaa gatccaaaga tggggacagc tccccgtatg acggtcgctg aaaatctttt
     7801 aatcgccaag tttcgtggtg agaagcgtgg attgttacca cgacgcttga ctagctataa
     7861 ggatgaattt caggcaacca ttgaaaaagt aggaaatggt cttgagaaac acttgaatac
     7921 accgattgag ttcttatcag gtggacaaag acaggctttg agtctcttga tggcaacctt
     7981 gaagcgacct gaattactcc tgttagatga gcatactgct gccctggatc caaagactag
     8041 tgttgctttg atggaattga cagatgaatt tgttaagaaa gatcagctaa cagcccttat
     8101 gattactcat catatggaag atgctctcaa atacggcaat cgcttgattg tcatgaaaga
     8161 aggacggatt atccaagatt taaaccaaga agaaaaagca aaaatgaaaa tctctgatta
     8221 ttatcaactc tttgaataaa gtagggaaaa tgagtgaaat ggtttacttt ttttctgaaa
     8281 taaagtatac tatataaagt aaactatgat aacatggagg tattgtgtat ggttgacaaa
     8341 caagtcattg aagaaatcaa aaacaatgcc aacattgtgg aagtcatagg agatgtgatt
     8401 tctttacaaa aggcaggacg gaactatcta gggctctgtc cttttcatgg tgaaaaaaca
     8461 ccttctttca acgttgtaga ggacaagcag ttttaccact gttttggttg tggtcgctca
     8521 ggtgatgtct ttaaattcat cgaggagtac caaggggtta cctttatgga ggctgtccaa
     8581 atcttaggtc agcgtgtcgg gattgaggtt gaaaaaccgc tttatagtga acagaagcca
     8641 gcctcgcctc accaagctct ttatgatatg cacgaagatg cggctaaatt ttaccatgct
     8701 attctcatga caacgactat gggcgaagag gccagaaatt acctttatca gcggggtttg
     8761 acagatgaag tgcttaaaca tttttggatt ggtttagcac ctccagaacg aaactatctc
     8821 tatcaacgtt tgtctgatca gtatcgtgaa gaggatttac tggattcagg cctgttttat
     8881 ctctcggatg ccaatcaatt tgtagacacc tttcacaatc gcattatgtt tcccctgaca
     8941 aatgaccaag gaaaggtcat tgccttctca ggtcgtatct ggcaaaaaac ggattcacaa
     9001 acttctaagt ataaaaacag ccgatcgact gcaattttta acaaaagtta cgaattatat
     9061 catatggata gggcaaaaaa atcttctgga aaaactagtg agatttacct gatggaagga
     9121 ttcatggatg ttattgcagc ctatcgggct ggaatcgaaa atgctgtggc gtcgatggga
     9181 acggccttga gtcgagagca tgttgagcat ctgaaaaggt taaccaagaa attggttctt
     9241 gtttacgatg gagataaggc tgggcaagcc gcgatattga aagcattgga tgaaattggt
     9301 gatatgcctg tgcaaatcgt tagcatgcct gataacttgg atcctgatga gtatctacaa
     9361 aaaaatggtc cagaagactt ggcctatcta ttaacaaaaa ctcgtattag tccgattgag
     9421 ttctacattc atcagtacaa acctgaaaac agtgaaaatc tgcaggctca gattgagttt
     9481 attgaaaaaa tagctccctt gattgttcaa gaaaagtcca tcgctgctca aaacagctat
     9541 attcatattt tagctgacag tctggcgtcc tttgattata cccagattga gcagattgtt
     9601 aatgagagtc gtcaggtgca aaggcagaat cgcatggaaa gaatttccag accgacgcca
     9661 atcaccatgc ctgtcaccaa gcagttatcg gctattatga gggcagaagc ccatctactc
     9721 tatcggatga tggaatcccc tcttgttttg aacgattacc gtttgcgaga agactttgca
     9781 tttgctacac ctgaatttca ggtcttatat gacttgcttg gccagtatgg aaatcttcct
     9841 ccagaagttt tagcagagca gacagaggaa gttgaaagag cttggtacca agttttagct
     9901 caggatttgc ctgctgagat atcgccgcag gaacttagtg aagtagagat gactcgaaac
     9961 aaggctctct tgaatcagga caatatgaga atcaaaaaga aggtgcagga agctagccat
    10021 gtaggagata cagatacagc cctagaagaa ttggaacgtt taatttccca aaagagaaga
    10081 atggagtaat aatggcaaca aaacaaaaag aagtaacaac atttgacgta caggtagcag
    10141 aatttatccg taatcataag caaaaaggga cagcaacaga tgatgaaatc aatgcaagtc
    10201 tggttattcc ttttaccttg gacgctgatg ggattgaaga tctcttgcaa cggattcagg
    10261 atgcagggat ttctatcaca gataacgaag gaaatccaag tgcgcgtgtt cttagcaatg
    10321 aagaagaacc agaactcagc gatgaggact tgattggatc aacttctgct aaggtcaatg
    10381 accctgtccg tatgtacttg aaagaaatag gggtcgttcc tctcttgacc aatgaagagg
    10441 agaaagagtt ggcactggct gttgaagctg gcgatatcga agccaaacaa cgtcttgcgg
    10501 aagccaatct tcgtttggtt gtttccattg ccaaacgcta tgtcggtcgt ggcatgcagt
    10561 tccttgactt gattcaagaa ggaaatatgg gcttgatgaa ggcggttgac aagtttgact
    10621 attctaaagg gttcaagttt tcaacttatg caacttggtg gattcgtcag gctatcactc
    10681 gtgctattgc ggaccaagct cgtaccatcc gtatcccagt tcacatggtt gaaactatca
    10741 ataaattggt tcgtgaacag cggaatctcc ttcaagaatt ggggcaagat ccgacaccag
    10801 aacagattgc tgaacgaatg gatatgacac ctgataaggt tcgtgaaatc ttgaagattg
    10861 cccaagaacc agtatctctt gaaactccta tcggtgaaga ggacgatagc caccttggag
    10921 actttatcga agatgaagtg attgaaaatc cagtggatta tacgactcgt atcgtcttgc
    10981 gtgagcaatt ggatgaaatc ttagatactc ttacagaccg tgaagaaaat gttctgcgtc
    11041 tacgttttgg actagatgat ggaaaaatgc gcacacttga agatgtgggg aaagtcttta
    11101 acgtaactcg tgagcgtatc cgtcagattg aagcaaaggc tttgagaaaa ctacgccaac
    11161 caagtcgcag caaaccgctt cgtgatttta ttgaagacta agagtgagga aaatatggct
    11221 tatacagaag agcaaattga aaacatcaaa acacggattt taacagcctt ggaagaagtc
    11281 atcgaccctg agttgggaat cgatattgtc aatcttggtt tgatctatga gattcgtttt
    11341 gacggtgaca cagggcaaac agagattgac atgactttga caactatggg ttgtccctta
    11401 gcagaccttt tgacagacca gatttatgat gctatgatag aggttccaga agtaacggat
    11461 actgaggtta aattagtctg gtatccagcc tggacagttg aaaaaatgag tcgctatgcc
    11521 cgcattgccc taggcattaa gtaaacatat aaaagcatgt gagagactag aggaaaagcg
    11581 aggaaatttc ccctcttttc ctctagtctc tcctttcttt tgctgatttt attcaaagaa
    11641 aatgatataa tagtagttat ggagaaaaag aaattacgca tcaatatgtt gagttcaagt
    11701 gagaaagtag caggacaggg agtttcaggt gcttaccgtg aattagttcg tcttcttcac
    11761 cgtgctgcca aggaccaatt gattgttaca gaaaatcttc caatcgaggc agatgtgact
    11821 cactttcata cgattgattt tccctattat ttatcaacct tccaaaagaa acgctcaggg
    11881 agaaagattg gctatgtgca tttcttgcca gctacacttg aaggaagttt gaaaattcca
    11941 tttttcttaa agggaattgt gaaacgctat gtattttctt tttacaaccg gatggagcac
    12001 ttggttgtgg tcaatcctat gtttattgag gatttggtag cagctggtat tccacgtgaa
    12061 aaagtgacct atattcctaa ctttgtcaac aaggaaaaat ggcatcctct accacaagaa
    12121 gaggtagtca gactgcgcac agatcttggt cttagtgaca atcagtttat cgtagtaggt
    12181 gctgggcaag ttcagaaacg taaagggatt gatgacttta tccgtctggc tgaggaattg
    12241 cctcagatta cctttatctg ggctggtggc ttctcttttg gtggtatgac agatggttat
    12301 gaacactata agaaaattat ggaaaatccc cctaaaaatt tgatttttcc aggcattgta
    12361 tcgccagagc ggatgcgcga attgtatgct ctagcggatc ttttcttgtt gcctagttac
    12421 aatgagctct ttcctatgac tattttagaa gctgcgagtt gtgaggctcc tattatgttg
    12481 cgtgatttag atctctataa ggtgattttg gagggaaatt atcgggcgac agcgggtaga
    12541 gaagagatga aagaggctat tttggaatat caagcaaatc ctgctgtctt aaaagatctc
    12601 aaagaaaagg ctaagaatat ttccagagag tattctgaag agcatctgtt acaaatctgg
    12661 ttggactttt atgagaaaca agccgcttta gggagaaagt aaaaagtgag gtaatctatg
    12721 cgaattggtt tatttacaga tacctatttt cctcaggttt ctggtgttgc gaccagtatt
    12781 cgaaccttga aaacagaact tgaaaagcag ggacatgctg tttttatctt tacgacgaca
    12841 gataaggatg tcaatcgcta cgaagattgg caaattatcc gcattccaag tgttcctttc
    12901 tttgctttta aggatcgtcg ctttgcctac cgaggtttta gcaaggcact tgaaattgct
    12961 aaacagtatc agctagatat tatccatact cagacagaat tttctcttgg cctgttgggg
    13021 atttggattg cgcgtgaatt gaaaattcca gtcatccata cctatcacac ccagtatgaa
    13081 gactatgtcc attatattgc taaggggata ttgatccggc cgagtatggt caagtatctg
    13141 gttagaggtt tcctgcatga tgtggatggg gttatttgcc ctagtgagat tgtccgtgac
    13201 ttgctatctg attataaggt caaggttgaa aaacgggtca ttcctactgg gattgaatta
    13261 gccaagtttg agcgtccgga aatcaagcag gaaaatttga aagaactgcg tagtaaacta
    13321 gggattcaag atggtgaaaa gacgttgctt agtctttcga gaatctccta tgaaaaaaat
    13381 attcaagcag ttttagcagc ctttgctgat gttctgaaag aggaagacaa ggttaaactg
    13441 gtagtagctg gggatggccc ttatctgaat gacctcaaag agcaagccca gaacctagag
    13501 attcaagact cagtcatctt tacagggatg attgctccta gtgagacggc tctttactat
    13561 aaagcggcgg atttcttcat ttcggcatcg acaagcgaaa cgcaaggttt gacctacttg
    13621 gaaagcttag ccagtggaac acctgtcatt gctcacggaa atccttattt gaacaacctc
    13681 atcagtgata aaatgtttgg agccttgtac tatggagaac atgatttggc tggtgctatt
    13741 ttggaagccc tgattgcaac accagacatg aatgagcata ccttatcaga gaaattgtat
    13801 gagatttcag ctgagaactt tgggaaacga gtgcatgagt tttatctgga tgccattatt
    13861 tcaaataact tccagaaaga tttggctaaa gatgatacgg tcagtcagcg tatctttagg
    13921 acagttttgt atcttccgca acaggtggtt gctgtacctg taaaaggatc tagacgcatg
    13981 ttgaaggctt caaaaacaca gttgatcagt atgagagact attggaaaga ccatgaagaa
    14041 tagaaagagg aacagctatg aaaaaaacaa ttaatgagaa gcggtcgtga taaaaagatt
    14101 gcgggtgttt gtgctggggt ggcccattat ctggatatgg atccgactat cgttcaagtc
    14161 atttggggtg ttcttacttg ctgttacgga gctggaattg tagcttacat tattttatgg
    14221 attatcgcac cagtagcaac tgactattga aaactagctc aattcatgct aagataatag
    14281 aaaatagaat gtaacgaagg agagaaaaat ggcatttgga gataatggaa atcgtaaaaa
    14341 aactatgttt gagaaaataa ccttgtttat cgtgattatc atgctagtag caagtttatt
    14401 gggaattttt gcaactgcaa ttggtgccct cagtaatcta taaaatagat tcaagaaaat
    14461 ttagtgactg ggatttccca gccctttttt aaagtgagaa gaaataatga gtatgttttt
    14521 agatacagct aagattaagg tcaaggctgg taatggtggc gatggtatgg ttgcctttcg
    14581 tcgtgaaaaa tatgtcccta atggaggccc ttggggtggt gatggtggtc gtggaggcaa
    14641 tgtggtcttc gttgtagacg aaggactacg taccttgatg gatttccgct acaatcgtca
    14701 tttcaaggct gattctggtg aaaaagggat gaccaaaggg atgcatggtc gtggtgctga
    14761 ggaccttaga gttcgagtac cacaaggtac gactgttcgt gatgcggaga ctggcaaggt
    14821 tttaacagat ttgattgaac atgggcaaga atttatcgtt gcccacggtg gtcgtggtgg
    14881 acgtggaaat attcgtttcg cgacaccaaa aaatcctgca ccggaaatct ctgaaaatgg
    14941 agaaccaggt caggaacgtg agttacaatt ggaactaaaa atcttggcag atgtcggttt
    15001 agtaggattc ccatctgtag ggaagtcaac acttttaagt gttattacct cagctaagcc
    15061 taaaattggt gcctaccact ttaccactat tgtaccaaat ttaggtatgg ttcgcaccca
    15121 atcaggtgaa tcctttgcag tagccgactt gccaggtttg attgaagggg ctagtcaagg
    15181 tgttggtttg ggaactcagt ttctccgtca catcgagcgt acacgtgtta tccttcacat
    15241 cattgatatg tcagctagcg aaggccgtga tccatatgag gattacctag ctatcaataa
    15301 agagctggag tcttacaatc ttcgcctcat ggagcgtcca cagattattg tagctaataa
    15361 gatggacatg cctgagagtc aggaaaatct tgaagaattt aagaaaaaat tgtctgaaaa
    15421 ttatgatgaa tttgaagagt taccagctat cttcccaatt tctggattga ccaagcaagg
    15481 tctggcaaca cttttagatg ctacagctga attgttagac aagacaccag aatttttgct
    15541 ctacgacgag tccgatatgg aagaagaagc ttactatgga tttgacgaag aagaaaaagc
    15601 ctttgaaatt agtcgtgatg acgatgcgac atgggtactt tctggtgaaa aactcatgaa
    15661 actctttaat atgaccaact ttgatcgtga tgaatctgtc atgaaatttg cccgtcagct
    15721 tcgtggtatg ggggttgatg aagcccttcg tgcgcgtgga gctaaagatg gggatttggt
    15781 ccgcattggt aaatttgagt ttgaatttgt agactaggag actggtatgg gagataaacc
    15841 gatatctttc cgagatgcgg atggtaattt tgtttccgcc gcagacgttt ggaatgaaaa
    15901 gaaattggaa gaactattta atcgtctcaa tccaaatcgt gccttgagat tggcacgaac
    15961 taaaaaggaa aatccatctc agtaaagaag ctaaaaaatc ccgtgcctca tcagacacgg
    16021 gattttgtgg tacgacaggc atgtatagca aactgaatct ggaatagcac agcatatctt
    16081 ctaaaatatt gttataaatt gatttgaatt tcaaaattaa attgtttgat tcttatctca
    16141 atttgttata gtatatctga tgtcaaagtt ctcggcgagt caaatagcga ttcccaagcc
    16201 tgactatcgt gaggtagcgg attaaaatgg tctggggata gaccgtttta agtctgacgc
    16261 tggaaataag aattgtcaga agaagggata gcgaaatcgt ggctctacga acaggaacgt
    16321 gataataagg cgtatatagc ggataagagg gcctcaaact ctaaagtcca aaaaggtagt
    16381 cgtaacctat atgcgtaaat cacgagagta attgaattcg tactaagatt ttctattttc
    16441 actgtaacct tttaacgtcc ttatatcttg tatacacgag gaaagatgta cgacttatcc
    16501 cgtgaggtct atcactataa agagaaaacg acagatagaa gtgatcctga gtcacggtta
    16561 tctttaaaat agattgtctg ataggacggt atgtataaaa cgcttctgtg aactgagaga
    16621 agggggagaa gttcttgcta aaatttagtt gaacagccgt attccgatac ttagataaga
    16681 gatctagtct tagctcctac tcagttttag gggataaaaa aggggaaata gcgattcgag
    16741 aaagattata ctcttcgaaa atctcttcaa atcacgtcaa tatcgccttg ccgtatgtgt
    16801 aggatactga ctacgtcagt tccatctaca acctcaaaac agtgttttga gcaacctgcg
    16861 gctagtttcc tagtttgatc tttgattttc attgagtatt agtaattcag ttactaactc
    16921 gtcaactctg atttatccaa taaaattgaa aaggatggaa aaaaggataa atttatgata
    16981 tactttattt tgaagacctt attagaaatc ttgaaagagt attgaaaact tagaatgaga
    17041 aaaattgtta tcaatggtgg attaccactg caaggtgaaa tcactattag tggtgctaaa
    17101 aatagtgtcg ttgccttaat tccagctatt atcttggctg atgatgtggt gactttggat
    17161 tgcgttccag atatttcgga tgtagccagt cttgtcgaaa tcatggaatt gatgggagct
    17221 actgttaagc gttatgacga tgtattggag attgacccaa gaggtgttca aaatattcca
    17281 atgccttatg gtaaaattaa cagtcttcgt gcatcttact atttttatgg gagcctctta
    17341 ggccgttttg gtgaagcgac agttggtcta ccgggaggat gtgatcttgg tcctcgtccg
    17401 attgacttac accttaaggc gtttgaagct atgggtgcca ctgctagcta cgagggagat
    17461 aacatgaagt tatctgctaa agatacagga cttcatggtg caagtattta catggatacg
    17521 gttagtgtgg gagcaacgat taatacgatg attgctgcgg ttaaagcaaa tggtcgtact
    17581 attattgaaa atgcagcccg tgaacctgag attattgatg tagctactct cttgaataat
    17641 atgggtgccc atatccgtgg ggcaggaact aatatcatca ttattgatgg tgttgaaaga
    17701 ttacatggga cacgtcatca ggtgattcca gaccgtattg aagctggaac atatatatct
    17761 ttagctgctg cagttggtaa aggaattcgt ataaataatg ttctttacga acacctggaa
    17821 gggtttattg ctaagttgga agaaatggga gtgagaatga ctgtatctga ggacagcatt
    17881 tttgtcgagg aacagtctaa tttgaaagca atcaatatta agacagctcc ttacccaggc
    17941 tttgcaactg atttgcaaca accgcttacc cctcttttac taagagcgaa tggtcgtggt
    18001 acaattgtcg atacgattta cgaaaaacgt gtaaatcatg tttttgaact agcaaagatg
    18061 gatgcggata tttcgacaac aaatggtcat attttgtaca cgggtggacg tgatttacgt
    18121 ggggccagtg ttaaagcgac cgacttaaga gctggggctg cactagtcat tgctgggctt
    18181 atggctgaag gtaaaactga aattaccaat atcgagttta tcttacgtgg ttattctgat
    18241 attatcgaaa aattacgtaa tttaggagcg gatattagac ttgttgagga ttaaaccgta
    18301 gaggtgttta tgaatatttg gaccaaatta gcaatgtttt ctttttttga aacggatcgc
    18361 ttgtatttgc gtcctttctt ttttagtgat agtcaggact tccgcgagat agcttcaaat
    18421 ccagaaaatc ttcaatttat tttcccaacg caggcaagtc tggaagaaag tcaatatgca
    18481 ctggccaatt actttatgaa gtcccctttg ggagtgtggg caatttgtga ccagaaaaat
    18541 caacaaatga ttggttctat taaatttgag aagttagatg aaatcaaaaa agaagctgag
    18601 cttggctatt ttttgagaaa agatgcttgg tcgcaaggat ttatgacaga ggttgttaga
    18661 aaaatttgtc agctttcttt tgaggaattt ggcttaaaac aattatttat cattacccac
    18721 cttgaaaatg aagctagcca aagagttgct cttaagtctg gatttagttt gttccgtcag
    18781 tttaagggaa gtgatcgtta cacaagaaaa atgcgggatt atcttgaatt tcggtatgta
    18841 aaaggagagt tcaatgagta agcatcagga aattctaagc tatttggagg aattaccagt
    18901 aggtaaaagg gtcagtgttc gtagcatttc gaatcatcta ggagttagtg atggaacagc
    18961 ctatcgagct attaaagaag ctgaaaaccg tggaattgtg gagacccgtc ctagaagtgg
    19021 aacaattcgt gttaaatccc agaaagttgc tatagataga ttaacgtttg ctgaaattgc
    19081 agaagtgact tcttctgagg ttctggctgg gcaagaaggt ttagagagag aatttagtaa
    19141 gttttcaatt ggtgccatga ctgaacaaaa tatcttgtct taccttcatg atggggggct
    19201 cttgattgtc ggagaccgaa cccgtattca gttgctagcc ttggaaaatg aaaatgcagt
    19261 tctggttaca gggggatttc aggttcatga tgatgtgctt aaactggcca atcaaaaagg
    19321 gattcctgtt ctaagaagta agcatgatac ctttaccgtc gctaccatga tcaataaagc
    19381 cttgtcaaat gtccaaatca agactgatat tctgacagtt gagaaacttt atcgccctag
    19441 tcatgagtat ggttttctga gagagacaga tacagttaaa gattatttgg acttggttcg
    19501 taagaatcgt agcagccgtt tccctgttat caatcaacat caggtcgttg ttggcgttgt
    19561 aaccatgaga gacgctggtg ataaatcacc aagcacgaca attgataagg ttatgtctcg
    19621 tagtctattt ttggttggat tatcgacaaa tattgccaat gtgagtcaac ggatgatcgc
    19681 agaagacttt gaaatggtac cagttgttcg aagcaatcaa actttgcttg gcgttgtgac
    19741 gcgacgagat gtcatggaga agatgagccg ttcccaagtt tcggctctac caactttttc
    19801 tgagcagatt ggacaaaagc tctcttatca ccatgatgaa gtagtcatta cagtggaacc
    19861 ctttatgcta gaaaaaaatg gagttttggc taatggtgta ttggcagaaa ttctgaccca
    19921 catgacccaa gatttagttg ttaatagtgg tcgcaatctc attatcgagc agatgctgat
    19981 ctactttttg caggccgttc agatagatga tatattgcgc attcaggcac ggattattca
    20041 tcatacgaga cggtcagcta taattgatta cgatatttat catggtcacc agattgtttc
    20101 aaaagcaaat gtgactgtta aaattaatta gaaactagga gaaaagatga taacattaaa
    20161 atcagctcgt gaaatcgaag ctatggacaa ggctggtgat tttctagcaa gtattcatat
    20221 aggcttacgt gatttgatta agccaggcgt agatatgtgg gaagttgaag aatatgtccg
    20281 ccgtcgttgt aaagaagaaa atttccttcc acttcagatt ggggttgacg gtgccatgat
    20341 ggactatcct tatgctacct gttgctctct taacgatgaa gtggctcacg ctttccctcg
    20401 tcattatatc ttgaaagatg gtgatttgct caaagttgat atggttttgg gaggtcccat
    20461 tgctaaatct gacctaaatg tctcaaaatt aaacttcaac aatgttgaac aaatgaaaaa
    20521 atacactcag agttattctg gtggtttagc agactcatgt tgggcttatg ctgttggtac
    20581 accgtccgaa gaagtcaaaa acttgatgga tgtaaccaaa gaagctatgt acaagggtat
    20641 tgagcaagct gttgttggaa atcgtatcgg tgatatcggt gcggctattc aagaatacgc
    20701 tgaaagtcgt ggttacggtg tagtgcgtga tttggttggt catggtgttg gcccaactat
    20761 gcacgaagaa ccaatggttc ctaactatgg tattgcaggt cgtggactcc gtcttcgtga
    20821 aggaatggtc ttaaccattg aaccaatgat caatacaggc gattgggaaa ttgatacaga
    20881 tatgaaaact ggttgggcgc ataagaccat tgacggtgga ttgtcatgtc agtatgaaca
    20941 ccaatttgtc attacgaaag atggacctgt tatcttgact agccaaggtg aagaaggaac
    21001 ttattaataa aaagtgaaaa gactactgga agtttattat gaaaaaatcc agtagatctt
    21061 ttcataataa aacgcattgt atcaagtgtt aggggctgat atcatgcgtt tttctgcttt
    21121 taagattttt tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc
    21181 tctcctccta tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc
    21241 tattgttcct gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat
    21301 tctgtttaat gttcgtattg ccaatcttaa atttaccttt tttagattag acttcctgcg
    21361 aaacaaaaca tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc
    21421 acgatttaag cgtcttgttg gtgttcaacg cacgactttt
//
LOCUS       DAVBCB010000037        21312 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVBCB010000037 DAVBCB010000000
VERSION     DAVBCB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21312
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.037"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(57..176)
                     /locus_tag="VQU28_001355"
     CDS             complement(57..176)
                     /locus_tag="VQU28_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2476704.1"
                     /translation="MDFKSFIIGLVVGIFGPYMYNLIRKIFSKFPKKDTDSTP"
     gene            complement(179..409)
                     /gene="blpU"
                     /locus_tag="VQU28_001356"
     CDS             complement(179..409)
                     /gene="blpU"
                     /locus_tag="VQU28_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HET2476705.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAARGLQ
                     LGIKTGTWQGAATGAAGGAILGGVAYAATCWW"
     gene            complement(478..747)
                     /gene="blpJ"
                     /locus_tag="VQU28_001357"
     CDS             complement(478..747)
                     /gene="blpJ"
                     /locus_tag="VQU28_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HET2476706.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(1214..1411)
                     /gene="blpI"
                     /locus_tag="VQU28_001358"
     CDS             complement(1214..1411)
                     /gene="blpI"
                     /locus_tag="VQU28_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HET2476707.1"
                     /translation="MNTKMMEQFSVMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            1693..2280
                     /locus_tag="VQU28_001359"
     CDS             1693..2280
                     /locus_tag="VQU28_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2476708.1"
                     /translation="MTSYKRTFVPQIDARDCGVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLATKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLIGHYYQYRWFLLSPRNLG"
     gene            2273..3862
                     /locus_tag="VQU28_001360"
                     /pseudo
     CDS             2273..3862
                     /locus_tag="VQU28_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
     gene            3873..5234
                     /locus_tag="VQU28_001361"
     CDS             3873..5234
                     /locus_tag="VQU28_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HET2476709.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMDESQKTAL
                     ETQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLANSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LIASQGQTQGTAEEPFLSQINQSIASLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLLLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     SIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5291..5446
                     /gene="blpC"
                     /locus_tag="VQU28_001362"
     CDS             5291..5446
                     /gene="blpC"
                     /locus_tag="VQU28_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HET2476710.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(5490..6830)
                     /locus_tag="VQU28_001363"
     CDS             complement(5490..6830)
                     /locus_tag="VQU28_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2476711.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNTYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(6844..7581)
                     /locus_tag="VQU28_001364"
     CDS             complement(6844..7581)
                     /locus_tag="VQU28_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476712.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKEHHITPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(7586..7924)
                     /locus_tag="VQU28_001365"
     CDS             complement(7586..7924)
                     /locus_tag="VQU28_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476713.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8201..8530
                     /locus_tag="VQU28_001366"
     CDS             8201..8530
                     /locus_tag="VQU28_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HET2476714.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(8698..9747)
                     /locus_tag="VQU28_001367"
     CDS             complement(8698..9747)
                     /locus_tag="VQU28_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476715.1"
                     /translation="MKDLFLKRKQAFRKECLCYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEAIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKASKFFTETG
                     LDWDYVISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(9744..10475)
                     /locus_tag="VQU28_001368"
     CDS             complement(9744..10475)
                     /locus_tag="VQU28_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476716.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            10543..10953
                     /locus_tag="VQU28_001369"
     CDS             10543..10953
                     /locus_tag="VQU28_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HET2476717.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMNATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            10963..11250
                     /locus_tag="VQU28_001370"
     CDS             10963..11250
                     /locus_tag="VQU28_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476718.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIIDQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            11412..11651
                     /locus_tag="VQU28_001371"
     CDS             11412..11651
                     /locus_tag="VQU28_001371"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476719.1"
                     /translation="MHHNLGNSYSIKIKKQTRKLGTDCSKHRFKVVDRTDEVSSKHCF
                     EVVDRTDEVSNHTHDKPTLTWFEEIFEEYHSPFHN"
     gene            complement(12001..13137)
                     /gene="dnaJ"
                     /locus_tag="VQU28_001372"
     CDS             complement(12001..13137)
                     /gene="dnaJ"
                     /locus_tag="VQU28_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HET2476720.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(13342..13695)
                     /locus_tag="VQU28_001373"
     CDS             complement(13342..13695)
                     /locus_tag="VQU28_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000115730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476721.1"
                     /translation="MSVNKNNLSDIADYHEKIKFFEELFNLDYNPTEMLVTVERNGNV
                     IYKEFTEVAVGKSKYDHTSVFKDVMIYFSDHICQELHLYKTQDPHYNKFEFDLAKRSL
                     KITTRDNTTIFISNE"
     gene            complement(13708..15531)
                     /gene="dnaK"
                     /locus_tag="VQU28_001374"
     CDS             complement(13708..15531)
                     /gene="dnaK"
                     /locus_tag="VQU28_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HET2476722.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(16011..16535)
                     /gene="grpE"
                     /locus_tag="VQU28_001375"
     CDS             complement(16011..16535)
                     /gene="grpE"
                     /locus_tag="VQU28_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HET2476723.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LGMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(16562..17596)
                     /gene="hrcA"
                     /locus_tag="VQU28_001376"
     CDS             complement(16562..17596)
                     /gene="hrcA"
                     /locus_tag="VQU28_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HET2476724.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFHRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTGLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(17760..18269)
                     /locus_tag="VQU28_001377"
     CDS             complement(17760..18269)
                     /locus_tag="VQU28_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HET2476725.1"
                     /translation="MNSRVEFRIFTIVDLDKEEEYLHEMHLKGWRYRTSRFGLFYFDQ
                     CQPDDVIYRIYDSRFLKKYKHELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTTTFLGGLVVCTSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            18877..20814
                     /locus_tag="VQU28_001378"
                     /pseudo
     CDS             18877..20814
                     /locus_tag="VQU28_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            20812..21312
                     /locus_tag="VQU28_001379"
                     /pseudo
     CDS             20812..21312
                     /locus_tag="VQU28_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         6698 a         4437 c         3832 g         6345 t
ORIGIN      
        1 attaaagaaa ctcccctgta aattaagcta gcaaatacag gggagaaatt tattttttag
       61 ggggtactat ctgtatcctt tttgggaaat tttgaaaata tttttctaat taaattatac
      121 atataaggac caaatatacc aactactaaa ccaataataa aacttttaaa atccataatt
      181 accaccaaca tgttgctgca taggctacac ctccaagtat agctccaccc gcagcaccag
      241 ttgctgcacc ttgccatgtt cctgttttaa tgcctagttg aagacctctt gctgctgctc
      301 ctccaacacc tgctttggca aaatctcccc aattgcatcc gccaccttca acgcaagcaa
      361 gcatttcagt atccataaca gaaaattgtg acatcatttt tgtatccatg acaaatactc
      421 cttttttaaa aaactaaaat aaatcagaat agaatcctca taattttact ataagtctta
      481 ccaacttagt cccaatttat caccaaccat acctcctaag catgttaatc cacccccaat
      541 tgcaccaatg tgtgctccaa caaatgcacc agcaagtcca gctactccta aagtggccaa
      601 acctgctcca gttccaccag ttataattcc cgtagtgact cctgtaatca gtgcattttg
      661 acaatcagtg gagctatacc ccccttcaac tttcgcaagc atttcagtat ccataacctc
      721 taactgtgac aacatttttg tattcatgat gaatacctcc tttttatttt caatttgtta
      781 ccaaagtctt aaattcaata aacaaataga ttttttatag tatctttttg attttcttaa
      841 aaaagtatat acgtctacta tcttcttaaa ggtagcagta cctatttttt agtctaagat
      901 ttcaataatc ttgagtatct aaaatatctt aatttcgtta ttctccttgc aataaaaagt
      961 tttactatac tatttattaa cttgcagaaa gcaaaaaata ttagtaaata atagtttata
     1021 gttaagtttt ttattcctac caatccatca actaagtaaa gcatcaacga ttacataaac
     1081 gattgataat ataattaaaa ttttgctaac tatcttattc tcatcattct tagataactt
     1141 tgatattttg taagtaagta aataagacag taaattaata gcgataataa tactatattt
     1201 aagaatcata atcttacaaa gaggacataa ttcctgaacc tacacaaata agtgttgctg
     1261 ctcccccagt tatcggacca gtcgcagcag ctaatagtac tgctccaata caaccaccga
     1321 ttgcagatcc taaattgcct cttcctccac taactatttc gagttcttca ttatccataa
     1381 cagaaaattg ttccatcatt tttgtattca tgacaaatac tccttttttc tttttttatt
     1441 tttgtcttgt tgtaactttg ataagtttag tatatcatcg ttttttaaaa tttttcatcc
     1501 agatcttgaa ttgtcatcga aacgtcttga attagctttt ttatttcaag ccacctctaa
     1561 atgtttaaaa aaaataattt ctaatcactt ttttaccatt caggaagttt taatgactat
     1621 tcaagatttc ataaaatatg aacttagttt tatgacataa tagacctatc cactatatga
     1681 aaagaattgc caatgacttc ttataaacgt acatttgttc ctcaaataga tgcgagagac
     1741 tgtggtgtcg ctgccttagc ctcgattgct aaattctatg gttcagattt ttctctagct
     1801 cacttgagag aacttgcaaa gaccaataaa gaagggacga ctgctcttgg cattgtaaaa
     1861 gccgctgatg aaatgggctt tgaaacaaga cctgttcaag cagataaaac gctctttgac
     1921 atgagtgatg tcccctatcc atttatcgtt cacgttaaca aagaaggaaa actccaacat
     1981 tactatgttg tctatcaaac aaagaaagac tatctgatta ttggtgatcc tgacccttct
     2041 gtaaaaatca ctaaaatgtc aaaagaacgc tttttctatg aatggactgg agtagctatt
     2101 tttctagcta ccaaacccag ctatcaaccc cataaagata aaaagaatgg tctactaagc
     2161 aagcttcctt cctctgattt tcaaacaaaa atctctcatt gcttacattg ttctctcaag
     2221 cttattggtc actattatca atataggtgg ttcttactat ctccaaggaa tcttggatga
     2281 atacattcca aatcagatga aatcaacttt aggaatcatc tcagttggtc tggttatcac
     2341 ctaggttatc acctatatcc tccaacaagt catgagcttc tccagagatt atctcctaac
     2401 cgttctgagc cagagattaa gcattgatgt gattttatcc tatattcgcc atatttttga
     2461 acttcccatg tctttctttg cgacacgtcg tacaggagaa atcatttcac ggttcacaga
     2521 tgctaactct attatagatg ccttggcttc taccattctt tctctttttc tggatgtttc
     2581 tattctgatt cttgtagggg gagtcttgct ggcacaaaac cctaatctct tcctcctttc
     2641 tcttctttcc attcctatat acatgttcat catcttttct tttatgaaac cttttgaaaa
     2701 aatgaaccat gatgtcatgc aaagtaattc tatggttagc tctgccatta tcgaagatat
     2761 caacgggatt gaaactataa agtcgctcac gagtgaagaa aatcgctatc aaaatataga
     2821 cagcgaattt gtagattatt tggaaaaatc ctttaagctc agtaaatatt ctattttaca
     2881 aacgagttta aagcagggaa caaaattagt tctgaatatc cttatcctat ggtttggcgc
     2941 tcaattagtc atgtcgagta aaatttctat cggtcagctg attaccttta acacactttt
     3001 ttcttacttt acaactccta tggaaaatat tatcaacctc caaaccaaac tcccatctgc
     3061 gaaggtcgct aataaccgtt tgaacgaagt ctatctagtc gaatctgaat ttcaagttca
     3121 agaaaaccct gtccattcac attttttgat gggcgatatt gaatttgatg acctttctta
     3181 taagtatggt tttggacgag ataccttaac agatattaat ctcacgatta aacaaggaga
     3241 taaggttagc ctagttggag ttagtggttc tggtaaaaca actttagcca aaatgattgt
     3301 caatttcttt gaaccctaca aagggcatat ttccatcaat catcaggata ttaaaaacat
     3361 tgataaaaaa gtcttgcgcc gtcatattaa ttacctaccc caacaagcct atatctttaa
     3421 tggctctatc ttggaaaatt taaccttggg cggtaatcat atgattagcc aagaagatat
     3481 tctaagagct tgtgaattag ctgaaatccg tcaagacatt gaaagaatgc ctatgggcta
     3541 tcaaactcag ctctctgatg gagctggtct atcaggagga cagaagcaac gaatagccct
     3601 cgctcgtgct cttttaacta aatctcctgt tttaatacta gacgaagcta ctagcggtct
     3661 tgatgtcttg actgagaaaa aagttataga taatcttatg tccctaactg ataaaaccat
     3721 tctctttgta gcccatcgtc tcagtatagc cgaacgaact aaccgtgtca ttgttcttga
     3781 ccaggggaaa atcattgaag ttggtagtca ccaagagtta atgcaggcgc aaggcttcta
     3841 ccatcatctg ttcaataaat aaggagaatg tcatgaatcc taatcttttt agaagcgtcg
     3901 agttttatca gagacgttac cataactatg cgacagtgtt aattatacct ctttcattac
     3961 tgtttacttt catcttgatt ttctcccttg ttgccacaaa agaaattact gttacttccc
     4021 aaggagaaat cgcccctaca agtgtcattg catccattca gtcaaccagt gataatccta
     4081 tccttgctaa tcatttagtg gcaaatcaag tagttgaaaa aggtgactta ctcatcaaat
     4141 actctgaaac aatggatgag agtcagaaaa ctgccttaga aactcaatta caaagacttg
     4201 agaagcaaaa agaaggactt ggaattttga aacaaagttt agaaaaagcg actgatcttt
     4261 tttctggcga ggatgagttt ggctaccata atacctttat gaattttact aaacaatccc
     4321 atgatattga actgggtatc acaaagacta acaccgaagt ttcaaatcaa gctaatcttg
     4381 ccaatagcag ttcatcagcc atcgaacaag aaattacaaa agttcaacaa caaattggag
     4441 aatatcaaga gttgagagat gctatcataa ataacagagc acgcttacca actggcaatc
     4501 cgcaccagtc aattttaaat cgttatctta tagcctctca aggacaaaca caaggaacgg
     4561 cagaggagcc atttttatct caaattaatc aaagtattgc aagtcttgaa tcatctatcg
     4621 caagcctcaa aattcagcaa gctggtatcg gaagtgtagc aacttatgat aatagtttag
     4681 caaccaaaat tgaagtactc cgcactcagt ttttacaaac agcctcacag caacaactaa
     4741 ccgtggagaa tcaattaaca gaattaaaag tacaactaga tcaagcgaca cagcgcttag
     4801 aaaataatac cttaaccgct ccaagtaaag gtatcgttca tctgaacagc gaatttgaag
     4861 gtaaaaatag aattccaact ggtacagaaa ttgctcaaat attccctgtc atcacagata
     4921 caagagaagt actaatcact tactacgtat catctgacta tctcctctta ctagataaag
     4981 gacaaactgt aagattaaaa ctggagaaga ttggaaatca cggcaccacc atcatcggcc
     5041 aacttcagag tattgatcaa actcctacca gaacagagca aggcaatctc tttaaattaa
     5101 ccgctcttgc aaaactatct aacgaggata gtaaactcat ccaatatggc ttacaaggtc
     5161 gcgtcactag tgtaactaca aagaaaacat attttgatta tttcaaagat aaaattttaa
     5221 cacattctga ttaattttca gataacactc tataactatt tattatctta tcaaaaagga
     5281 gaatcataac atggataaga aacaaaacct aacttcattt caagaactaa caactaccga
     5341 actcaaccaa attataggtg gaggatggtg ggaagatttc ttatatagat ttaatataat
     5401 tgaacaaaaa aatacaaaag gatttcatca gccaatacaa ctataaaaaa acaagaccaa
     5461 gagaacgcac tctcggtctt attttttctt cattctatat gtattacagt aagtacctga
     5521 cgaaaaacgt gatcttggca ggtggtattg agactggtat tgggatgact ttccacaatt
     5581 ttcataacgg tatagagacc aactcctctc tcctcccctt tagaacttgc tccaaaggag
     5641 aagatttcag aaatatcgat tccctcttct ttgatggaat tttcaatgat aaaggtctcc
     5701 tgtgctccat tttttaaaaa ggcaattgaa acatgaggtt gacaggcttc tacactagct
     5761 tcaatagcgt tgtcacaaag gatagacaca acggttagaa aatcaagtag actcatcccc
     5821 tctacctgaa tctcctctgg aacttcgaca ttaaagacaa tgttcttatc tctagctttt
     5881 agaaatttcc ctgctaggag acttttgagg gctttatcac gaatattcac caatcgacca
     5941 aggtcatatt tattgtcctg caatttttca ctagaatcct ttaagaccga gtcgtagatc
     6001 tcttttatct gctccatatc ctcctcttca atgcccagac gtaagctagt caagaggttg
     6061 gtgtagtcat ggcgaaaact ccgtacttcc ttataaagct cctctatatg ccgactatac
     6121 cgctccatct ctctgtagcg caagtcctgc tcttggttca gtctctcatg gagtttgtcc
     6181 ttcaaatagg tatccaattt cttgataatc cccataaaaa agagcaggta aaagactagg
     6241 atgagatggc gaacagtcgt tgattgaatc ccctgttcat attcaaagta agacagattt
     6301 tgtatcacca aatagtaagc ccccattatc cagttaatct gagtcaggga cttttgaaaa
     6361 tctttatcga gaatcccctt tctcaagcta gtgaaatcat agtccaacca tttcaaaaaa
     6421 gctagagaaa tgaagaaatt gaaaattatt atacataacc aaataaatga gtagtcatca
     6481 tatacttgcc cttgtcccaa aaatggaagc acaaaatagg aaacacctct ataaaagaga
     6541 ttcactaata tcattggaaa gagaccataa aagagaagga gttttttagg aagttctctt
     6601 aacaataaga aagataagcc tatgccgtac aagggttcca taaaataaga tagataagta
     6661 tttcctacca tatagccaag cattccaaaa acaacggcca acagtatctt aaaaagaaag
     6721 gccttaaaaa tcctctcaaa agtgagacta attccatcta ccttaaagaa tatgacaatt
     6781 tttaatccgt gagtaacaag tgtatacaac aatatccaag caacattcat aaactctcct
     6841 agctcagtgt aagttattaa tagcctcaga cacttccctg accttataac gcgcgattag
     6901 acaacttcca ccattgggaa agaaaagcag tttttctttc ttatctaaat gcaccacatt
     6961 ggcaggattg atgagaaaag agcgatggca ctgcaagaga cgaggctcct gcttgagaac
     7021 ctcctctaga ctcgctgtaa attccagcct gtctgtcttg gtatagagaa taacacgatg
     7081 gggtcttgtg gacgtttcga gatagtaaac ctctttaaaa ggatactgga attgagcaaa
     7141 ttttgattta aagtaaaagc aatcttccgc tagactttta ctgtcttgac cattggcata
     7201 gaggagggct gtctcgatac gagattcaaa ctcctctgcc gaaagggcct tatcaatata
     7261 gtccagagca gacacttggt agcgaaagga caggggcata aactccgagt gagtcgtcac
     7321 aaagacaatc agggcatacg gatcccgatc ccgaatcttt ctagccactt ccagaccctt
     7381 catctcctca tttcgaatct caatatccaa aaagaatagc tgatgggccc ccttctcatg
     7441 cacctctgcc agcagttggt ctggcttgcc aaatacctca aaagagctgg gagtgatatg
     7501 atgttccttc aagagtttct caatcgtcgt ttcaatccta gtctgttggg aaaaatcatc
     7561 ctctaaaaca aatattctca tcttcttact ctccctcttt caaccatttt tgacgaattt
     7621 ctctatagcg acgtctggca tacttgacag cgtattgacc ttcttctccg agaaaaagga
     7681 ttctttcttg aaaatcaatc gattttaatt tatcaagatt aaccaaacaa tttcgatgac
     7741 atctcatcaa ggtttcccca tattggttct caagattact taaattttga agcatattaa
     7801 aactagcttc ttctgtaaca atctgtacgg tatgggcttt agttggatgt gtttggatat
     7861 agtagatatc gtcgacgttt actttataga ctgttttctg cgtctgaata atcatatgtt
     7921 tcattggatt ctcctcctaa gaaaagaata acagaaaagt tctagcccaa gcccacccaa
     7981 tcctaaacag tctttgaaag ctcctaaaag gattattttt ctacaacatc atgatgagct
     8041 tacaactatt ttatcataaa attcactcag cgcaattcaa gacatttcaa tgacaattaa
     8101 agatttgaat aaaaaaaaag aattaaaaat gataaactaa cagtatcaaa acacagttgc
     8161 cagaagtaag actggcaccc agattaaagg aggcattccc atgacagata cagaccccat
     8221 caaaagagct catactttga ttactgactt aaacaaagcc taccaagcat gcaaacaggc
     8281 aagcgctgac gacgtccgct ttcaggagca attaaactct attcttggtt ttttagccaa
     8341 ggctgaaaca gtggataatc gattcttgat tgaactagaa aaattttacc agacttccag
     8401 tcttctcatg ggactcagcg ctcttgatcc agatgctcca actcgcgccg cttggcgggc
     8461 ctatgaccgc ttccactttg accaagtcaa gaccaagtta atactcaatg aaaatcaaag
     8521 agcaaactag gaagctagcc gcaagctgta cttgagtacg gtaaggcgac gctgacgtgg
     8581 tttgaagaga ttttcgaaga gtataagtct ctacggacca accattatcc tgtagtaaca
     8641 aaaaagaagc tgagacagac tgaactcaac ttctttttta gtgtcgtatc agcaatgtta
     8701 gtcctgcatc tgacgtttta cctgataagg caaatacaag actagtaaaa ccaaaccagc
     8761 tcctagtaag gctagaaggg ccagtttttc ttggaaggta atcaacccaa caactaattc
     8821 cacaagtaaa acaaaactgg tcaatcctcg aactacctcc tgtaagcctt tttccttttg
     8881 ccccttgtcc agcggaaaga gttgggtcaa atactggtag tcaaaggcat gatagagggc
     8941 cagcaactgg aagagcaaga ggtagttaaa gagaactacc actgctgtcg caatccaagc
     9001 ttgctcgata aaaacctgcg ccagtacgga aagtaataga agacgaagac tgagagcaaa
     9061 gaggtcgcca tttcgcagat aagaacgcag atagagattt tgccaaatct tcccaggcac
     9121 cttctgaaca gcctttaaga taaagtccag ataggcacga cgcttaacgc tgtttgaaat
     9181 tcccttgacc tgcgtaaaga gggcaaagaa acgaagcaag acttgcttac gcttgctttc
     9241 ttgagaaata acatagtccc agtccagtcc agtttcagta aaaaatttgc tggccttttg
     9301 acgaaagtgg aaatattttc ctacccccaa taaaagcaca tatagcagaa aaactggcaa
     9361 gccataaccc attgctaaaa ataaaggcgc aaataacagc aagaaaaggg tctgtacaaa
     9421 gagccaaaag actagggaaa tgccagtttg acgcttgaga tggagcttga ttgcctcttc
     9481 tccaactaag agaaagagct tgtctggagc ctccatatag gtggcaattc ctccccaaag
     9541 taagagtaaa acagacgtaa ttcctacaaa caaaaggata ggccaatgat tttcaggaaa
     9601 atgttgtaag agttgactgt actggtaggc tagaaagccc aacaggacaa gcaggaacaa
     9661 gacaaagtgg tcattgagca catagcgcag ataacaaaga cactccttac gaaaggcctg
     9721 ctttctcttt aaaaacaagt ctttcataga tcctcctctt tggtcagagc caagtaaata
     9781 tcattcaaac tagcctcagg catatcaaag gcttcacgta gttgcaggag attgcctttg
     9841 gcacgcacct ctcccttgtg aagaatgaca aaggcatcac acatcttttc cgccgaatcc
     9901 agcacgtggg tactcatgag aatagacttg cccttttgct tctccacttc caaaagctga
     9961 atcaaatcag aaatagccag cggatcaaga ccaaggaaag gctcatccac gatgaaaaga
    10021 cttggatcca ccacaaaagc acagataatc atgaccttct gcttcatccc ttttgaaaaa
    10081 tgaacaggga accagtctaa tttctgttcc aaacggaaca tttttaacaa gggctctact
    10141 cgttcgaaag ccactttttg ctcaataccg taagccatag caaccgtttc gatatgctct
    10201 ctgagggtca attcctcata cagactaggc gtctcaggaa tgtagccaat ctgcttgcgg
    10261 tagctagtcg catctccttg cagagtcagg ccattgatat tgatggagcc actataaggt
    10321 gccaacagac cgataatctc attgatcgtc gttgatttcc cagcaccatt gagaccaatc
    10381 aaaccgacca actgcccact ttcaacagta aaggacacat ctttcaaaac aggaacatga
    10441 acatagccac ctgtcaggtt tttaatttct aacatatttt ctccaaatct ggtataatgt
    10501 agctatatta tatcaaaatt cagtacagta gaggtagatt ttatgtcaga ttgcattttt
    10561 tgtaaaatca tcgcagggga aattcctgct tcaaaagtat atgaagatga gcaggtcctt
    10621 gcctttcttg atatctctca agtaacacta ggacacacct tggtcgtgcc aaaagaacac
    10681 tatcgcaatc ttttggagat gaatgctacg agcgccagcc aactctttgc ccaagtacca
    10741 aaagtagctc aaaaagtcat gaaagtcact aaggctgctg gtatgaatat catttccaac
    10801 tgtgaagaag tcgctggtca aacagttttt catactcacg ttcaccttgt gcctcgctac
    10861 agtgctgacg atgacctcaa gattgatttt atcgcccacg aaccagactt tgacaaactt
    10921 gctcaagtcg ctgaaaccat caaaaacgct taaggagttg ctatgaaatt atccaaccta
    10981 ctgctatttg caggagctgc agccggaagt tatctggtta caaaaaatcg ccaaaccatc
    11041 acagatgaag tcttgaatac cactgaccgc gttcaagcta tcaaagacga tgtggatatt
    11101 atccaaaata gcctgcaaat catcgaccag caaaaagaac ttatcaagga ataccaagaa
    11161 gacttgactt acaagtttaa ggtcttggaa aaggatatcc agactagact agctgtgata
    11221 aaagaaatgc agggaactga agataagtaa aaagagcccc ccggctcttt tttaaaacgt
    11281 aaacaagtta tagtgaattg aatttagaat agcacatcat agtttctaaa gcattgttag
    11341 agattacttt aaaatcctct tatcaatttg ttcatattct atttcaatct actatatata
    11401 tcccatcaac tatgcatcat aatttaggta actcatactc aataaaaatc aaaaagcaaa
    11461 ctagaaagct aggcacagac tgctcaaaac accgttttaa ggttgtggat agaactgacg
    11521 aagtcagctc aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata
    11581 cccatgacaa gccgacgctg acgtggtttg aagaaatttt tgaagagtat cattcaccat
    11641 ttcacaacta aatcgaagac tttctgccgt aatgaaaaca cctgaaacag cttggtttca
    11701 gctgtccgga aactttaaga cttaggttca aagtttaggt atggaacttc gaaggaggtc
    11761 gctaccgtcc gtcattactt aggaaagtct taaaaaatct ataaagtaaa aagagcccga
    11821 cagctctttt tgtttatact caatgaaaat caaaaagcaa actaggaaac tagccacggg
    11881 ctgctcaaaa cactgttttg aggttgtaga tacgactcac atatatacgg caagccgacg
    11941 ctgacgtggt ttgaagagat tttcgacgct gacgtggttt gaagagattt tcaaagagta
    12001 ttattctcca tcaaaggcat ctttaatatg gtcaaagaag ccttttttct ttggatttac
    12061 tttcaagtca ccagcagccg cgaattcttt caaggctact ttttggcggt cgttcaagcc
    12121 tgtcggtgtt acgacattaa cagtaacgta ttggtcacca actgcaccgc cacgaaggct
    12181 cggtgccccc ttactacgta ggcggaattt cttaccagtc tgagttccct ctggaataac
    12241 caattcaaca tcaccgtgaa cagttggaat atctactgta tcaccaagag ccgcttggac
    12301 aaagttgagg ttgagattgt agaagatagt cgttccttca cgttcaaact tgtcgctagc
    12361 ttctacagaa actactacat acaagtcccc ataaggtcca ccgttaaagc ctgcttcgcc
    12421 ttgaccagcg aggcgaattt gttgacctgt ttccacacca gcagggattt tcacatgtac
    12481 gctatgagct tgtttctcat gacctgttcc atgacaggtt gtacatggat atttgatttc
    12541 ttttcctcga ccgtgacaga catcacaggt tacttggcga cgcatcatac caagaggagt
    12601 ctgcgtatcg acgttaatga caccagcgcc atgacagcgt ccacaagtga ctggacttgt
    12661 ccctggctta gcaccagagc cattacatgt acgacagcca gcttcacgat gatacttaac
    12721 ttccttctca gttccgaaga tagcttcttc aaaggtcaaa ttgacacgat actggagatc
    12781 atctccttgg cgaggagcgt ttggattgcg cgaagaaccg cctccgccga agaaacttga
    12841 aaaaatatcc tcaaaaccac cgaagccacc tgccccattg aaaccaccga aaccaccagc
    12901 tccaccaaaa ccaccattgg cgcctgcagc accatactgg tcataggcag cacgtttttg
    12961 gtcgtcactc aaagtctcat aggcttcttg aacttccttg tacttgtcct cagcaccagg
    13021 ctccttgttg atatctgggt gatatttttt ggaaagctta cgataagcct ttttgatctc
    13081 gtctgccgaa gcgttttttg acacccccag acgatcataa aattcagtat tgttcataca
    13141 agataccaag accgtaaaat tcgactgaaa aataggaaat ctgacgctgg agcgacgctc
    13201 ctagacagat ttctcttttt tccgagaatt taggtcgggt tcaattcctt tcttttatat
    13261 tgatgagata atactccagc cactcggccc tatccctttt taccgaaaaa cagaggctga
    13321 gttgcaacct ctgtggttag attattcatt tgaaataaaa atggttgtat tatctctagt
    13381 agtaatcttc aaagaccttt tagctaaatc aaactcaaat ttattatagt gtgggtcttg
    13441 tgttttatat aggtgtaact cctgacatat atgatccgaa aagtaaatca taacatcttt
    13501 aaacacagaa gtgtggtcat atttactctt accaacagct acttctgtaa attctttata
    13561 aatgacattt ccatttcttt ccacagtcac cagcatttct gttggattat aatccaaatt
    13621 aaataattct tcaaaaaact ttatcttttc atgataatct gcaatgtcag aaagattatt
    13681 tttattaaca ctcataacac tagcccctta tttttcagta aactctccgt ctacgacgtc
    13741 atcgcctgcg tttcctgttg cttgtgcgcc ttctgctcct tcttgagctt gttgcgctgc
    13801 tgcagcttgt tcgtagagtt taacagcaag tccttgagct ttttcgttca atgcttcaag
    13861 ttttgctttc atgtcgtcca agttgttgtc ttcttgagct ttcttaaggt catcaagggc
    13921 agcttgggca gcgtcacgtt ctgcgtcgaa gcctttacct tcagtttcct tgattgtctt
    13981 ttcagtcgca aagattgctt ggtctacttc attacgaagg tctacttctt ctttacgttt
    14041 cttatcggct tcagcgtttg cttctgcatc tttcatcatg cggtcgattt cttcgtcagt
    14101 caaacctgag ttcgattgga tgacaatagt ttgttctttt tgagttccaa ggtctttggc
    14161 cttaacagac acgataccgt tcttgtcgat gtcaaatgtt acttcgattt gaggaattcc
    14221 acgaggtgca gctgggatat cagtcaattg gaagcgtcca agagtcttgt tatctgctgc
    14281 cattgggcgt tcaccttgaa gaacgtggat atcaacggct ggttggttgt ctgctgctgt
    14341 tgagaagact tgtgatttag atgttgggat tgtagtgttg cgatcgataa gttttgtaaa
    14401 tactccaccc attgtttcga taccaagtga caatggcgtt acatcaagaa ggacaacgtc
    14461 cttgacatca ccagtaatca caccaccttg gatagccgca cccatagcaa ctacttcatc
    14521 agggtttact gatttgtttg gttctttacc agtttcagct ttaacagctt caacaacggc
    14581 agggatacga gttgaaccac caacaaggat aacttcgtcg atttctgaca agctcaaacc
    14641 tgcatctgaa agggcttgac gaactggaac ttttgtacgt tcaacaaggt cacgagtcaa
    14701 atcgtcaaat ttcgcacgag tcaaagtcat ttccaagtga agaggtccag cctcacctgc
    14761 agtgataaat ggcaagctga tttgtgttga agttacacca gaaaggtctt tcttcgcttt
    14821 ttcagccgca tctttcaaac gttgcattgc catcttgtca gtagacaagt cgataccgtt
    14881 ttctttcttg aattctgcta ccaagtggtc aatgattttt tggtcaaagt cgtcaccacc
    14941 aagtttgttg tcccctgcag ttgacaatac gtcgaagaca ccgtcaccca attcaaggat
    15001 agagacgtcg aatgtaccac caccaaggtc aaataccaag attttttctt ctttgtcagt
    15061 cttgtccaaa ccataagcaa gagctgctgc agttggttcg ttaacaatac gttctacttc
    15121 aagaccagca attttaccag cgtcttttgt tgcttgacgt tgagcgtcgt tgaagtaagc
    15181 cggaactgtg ataacagctt tggttacttt ctcaccaagg tagtcttcag cgtagccttt
    15241 caagtattga aggatcatag ctgagatttc ttgtggagtg tattcttttc catttgcaga
    15301 aactttttca gaagttccca tcttagattt gatagagata actgtatctg ggtttgtaac
    15361 tgcttgacgt tttgcagcat caccaacgat gatttctccg tttttgaatg agactacaga
    15421 tggagttgtg cggtttcctt ctgggtttgc gatgattttg ctttcagttc cttcaagaac
    15481 tgcaactgct gagtttgttg tacctaagtc aataccgata attttagaca tgtgtttttc
    15541 tccttgttag ttaattttct atttttcttt ttgatactct tcttaagtgg cgacgccgtc
    15601 agagcttgac ttcgtcaatc tctttgacta aactttgagc ctaaggtctc aaaatttgcg
    15661 caaatagcac cacggcgaag agtatcgtct ttggttcgct tcgctcacta ttacaaagcc
    15721 aaacatattt tatctttctt catagtttat tgtcgtttcg gacaagtttt ctattatgta
    15781 aattgcgaca cgaggagtcg aaatcgattt tatttcgacg acgagttagt aaggaagcta
    15841 ggcaaacgtc atagcgattg ccgttttctg acgagttgct ttagctaccg tcagaattgc
    15901 cgaatcgaac acgccctaag ctctgagtaa aaaagataga ttttcttgtg ttcatcgaac
    15961 acttcgtcaa tctcctattt ttactgcgag ctttttacga gctttgtatc ttagttatac
    16021 accactacca ttgctgggcg taggatgcgg tcatggagtt tgtagccttt ttgaaagact
    16081 tgggcgatgg tatctactgg gtgttcatcg tctgctggga gagtttggat ggccatatgg
    16141 tagttatggt caaattcgcc atctgctgcg atttcttcaa ttccttcttc tttcaaagcg
    16201 tgaatcaagc tttcttgcac catccccaag cccttcttca catcatctgt caaaccttca
    16261 actgcaagtg cacgctcaag gttgtcaaga gatggtaaga ttgcttttgc caagtcctgg
    16321 ctacgataac gttgcaagtt ttgacgttct tcattggcac ggcgttggat attttgcatt
    16381 tctgcatgag cgcgaagata tttgttttcg aactcatctg cacgttcatt tgccaagtcc
    16441 aactcagact tttcaggagt tgtttcttca gctgtttcca caacttcctc ttcttgaact
    16501 tcttctactt cttcattttt tatatcttgg gccatgttcg cctcctttaa tgatttcaat
    16561 cttaatgtac ttcgtaatga ttactgctga ggtagcggta aaaatctgtc aacttcatgg
    16621 tcaaaacacg gttgaccaca ttgacttgat tgattagctg ttggtaatcc agattaactg
    16681 gaccgataat ggctagaatt ccaactcccc gataaggaat gaggaactta ctactaatca
    16741 ccgctaggtc agctaaacag gactcttgac cgtctgcaac acgaacattt tgcatctgat
    16801 cctcacgcaa cccctcacga atctccaagg ccaccttttg cggttggtca aagaactgat
    16861 aggcttctag attggcaaaa ttcaagagat tgaccttgcc cgccatcaca atgttttcgt
    16921 tgaacatttc cttaaagatg tgttcaaaga gatcgatgac attatccgtt gttgtaaagt
    16981 aacgctggat aatctgcgga atctccgtcc gaatcttgta gtgaatatct aaaacggtgt
    17041 gaccgaggaa acgttcctga atgatgctct tcagtttcag caaatcctcc tgcaagaagt
    17101 tccttggaat cagaaactga ctagtaaccg ttcgcgactc gtctagggta aataccgcca
    17161 aggctgtatg ttgccccaaa acaacgatat caaaggctgt caaacgttgc ctgctcggct
    17221 caacatccag tgccactacc gtacagccac tcaggcctgt tagcaagtta gcagcctctt
    17281 gcagaatatc ctccaatttg aagaattcct gatcaaaggc tttgacaatc tcatatacct
    17341 cattttcagc cagccggtga aaatccagtg agtgtttcac atagtactga aaaccagcaa
    17401 cacttggcat ccgaccgctt gaagtatgag ccttctcaag caacccttgt ttttctagtt
    17461 ccgccatgtc attacgaatg gttgcactgc tagagttaat agactcttgc aaggcttttg
    17521 atccgacagg ttcgtgcgtt ttggtaaaga tgtcaataat cagatttaaa atatcctgct
    17581 gacgctctgt aaccatctca tcactcctct ctgtctgatc aattagcact caatgcatcc
    17641 aagtgctaac ttatgattct attatacacc cttaaaagag aatgtcaaga caaaaactca
    17701 aaaaattagc actcttttat caagagtgct aaaaatcaat ctgaagacct agaaccttgc
    17761 tattcatcta ctcggtattt cctttttagt ctgaaataac catgaattag ataagaaatc
    17821 agaaaagcat ataaaacaaa aataagaaag aaagacatag aaagatcctc tctaaacaaa
    17881 ctcgtacaaa caacaagacc acctaagaaa gtagttgtac aggaacgaag tctagatcgc
    17941 ataacttccc atttgagccc cttactcata tagacttgat cctctggaag taaatcagaa
    18001 gacgatttac gaagaatcga acaagaacct gctcctatca attcccaacc tctatctcta
    18061 aaatcttgca gttcatgctt atatttttta agaaatctag aatcatagat acggtagatg
    18121 acatcgtctg gttgacattg gtcaaaatag aacaaaccaa aacgactcgt tctatacctc
    18181 caacctttca aatgcatctc atgtaaatat tcttcttcct tgtccaaatc aacaatggtg
    18241 aaaatccgaa attctactct gctattcatt gtcttacccc aaaattagaa aacatgcctg
    18301 gcgttattta ttagataatt ctttccactt ttgactcaat ctccaaaaaa tataagaaat
    18361 ctgaatcgca aaaactatca ataaaaccca atctattatg aaaatcaaaa acactttcca
    18421 actgaaagaa ctacctccag tgacaaactt tgagcaaaac ggtagtagag ctaaaaagag
    18481 aagtaaaata ggaagcatcc gcattgttaa aatccgtttg gcataaaaaa atctttattt
    18541 aaactaaaat attatagcaa aatttacgcc agtttttgaa cggctgatgt agatatttta
    18601 tactttcaaa atgtttaaat gtgattattt atttttgaaa aatagctcac cagcccgact
    18661 gaaagtgctt atagaatgat aataagtcgc ctgccgaaaa cagcggaaaa tagcggtgtt
    18721 atgcggagat aatctgacgc gatgcgaaag tatattgcat acttattttc aacaatttag
    18781 cagagtattt ttataagtgt gatataatag aagtataatt tgttctgata gtttatttta
    18841 tggagaagta gatttttaga atgcggaggg ttcaatatgg ttgagtttat aaagtctaag
    18901 aaagaaatga gtgaggagga tattaaagca aatttcatca ctcctgctat tgtatccaaa
    18961 ggatggaaaa atggtgagca tatcgcttac gaagaatact tcactgatgg tcgaattgag
    19021 gttagaggag ataaggctcg tcgtaaagaa ggaaaaaaat cagactattc actgtattac
    19081 caatttggaa ctcgaattgc aattgttgag gcaaaggata ataaacacag cgttcgagca
    19141 ggattacaac aagctattga atatggagag attttagatg ttccatttgt ttattcttcg
    19201 aatggtgatg gctttattga acacgaccgt atcacgagag aagaacgtga gctggagtta
    19261 gacgaattcc ctactcgtga agaattattt tctcgtatga cgaaggaaaa aggattgacg
    19321 tacgaaatta cagaagctat ctcaactcca tactatacag acgccttctc aatgaaaacg
    19381 ccacgctatt atcagcaaat agctatcaac cgtactattg aaacagttgc cagaggacaa
    19441 aaacgagtaa tgtttgtgat ggcaacagga acagggaaaa cgttcatggc ttttcaaatt
    19501 attcatcgcc ttcgaaaagc tggtttggct aaacgagttt tattcttagc agatagaaac
    19561 atcttagtag accaaacgat ggctgaagac tttaagccat tcgaaaaggt aatgacgaaa
    19621 attacaccaa aacttttgac tgctcctgaa aaattaaatt ctttcgaaat ttatctaggg
    19681 ctttatcagc aactaactgg tgaagatgga actgaaacac attatcaaaa atttgacaaa
    19741 gacttctttg atttaatcgt aattgatgaa gcgcaccgtg gttcagctaa ggaaaacagt
    19801 aactggcgta aggtaattga ttatttcagt tctgcgacac agattgggat gaccgctact
    19861 cctaaagaaa ccaagaatgc ttccaatacg gaatactttg gtaagccaat ctatacttat
    19921 agtttaaaac agggaatcga ggatggtttt ttggctccat atcgtgttat gagggttaat
    19981 ttagatgtgg atgtggatgg ttatcgtcca gaaactggaa aagttgatgc taacggacaa
    20041 ttaatagaag ataggtacta cggcaggaaa gattttgata aaaccattgt cattgatgat
    20101 agaacgcaaa gagttgccaa gtttgtttct gattatatga agcaaaacaa tgcacgattt
    20161 gataaaacaa ttgttttttg tgttgatatt gaccatgccg agcgaatgcg tgctgcactt
    20221 gtaaaagaga atctagactt agtccaagaa gactatcgtt atgtcatgca agtaactggt
    20281 gacaacgctg aaggaaaagc tcaactggat aactttatgg atgtcaattc taattttccc
    20341 gctattgtaa caacgtctaa attattaacg acaggagtta atgctaaaac atgtcgtttg
    20401 attgttttag actctaatat ccaatccatg actgaattta aacaaattat tggtcgtggc
    20461 acacgtcttt atcctcaaaa ggggaaagaa ttttttacga ttattgattt tcgaaatgtt
    20521 accaatttgt ttgctgaccc tgattttgat ggtgagccag tgaaggtgct ggaaacaggt
    20581 gcgaaaacag tcagtggttc tacgcctggt ttcgtaggtg aggaaggtga cccagtagaa
    20641 aaatatatcg ttacagacaa gcaggttacc attcttaatt ctactgttca agtattggat
    20701 gaaaacggga aactgattac cgaaagcctg accgactaca ctcgaaagaa tatcttaggt
    20761 agctacgcca ctttgaacga ttttatcaca gtttggcata cggcagataa gaaacattat
    20821 gggaagtttg ctgatggaag cactcaagaa attgatgttc cttatgatat tcctgatact
    20881 tgggagtggg tgaggttttc tacattggtt gaaattgtca gaggtggctc tccacgacca
    20941 atcaaagatt atcttacttc tgaagtagat ggaataaatt ggataaaaat aggtgatact
    21001 gaaaagggtg aaaagtatat aaataatgtt aaagaaaaaa tcaaaaaatc agggcttaac
    21061 aaaactagat ttgtaaaaaa aggtacattt ttgttaacta attctatgag ttttggtaga
    21121 ccttatattt tgaatgttga tggtgcaata cacgatggat ggttggctat ttcgaactat
    21181 gaaaactcat taaataaaga ttacctattc tatattcttt catcaaatgt agtttattct
    21241 caatttctat ctctaattag tggagctgtt gtgaaaaact tgaatagtga taaagttgct
    21301 tctattctta tc
//
LOCUS       DAVBCB010000038        21310 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVBCB010000038 DAVBCB010000000
VERSION     DAVBCB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.038"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            40..384
                     /locus_tag="VQU28_001380"
     CDS             40..384
                     /locus_tag="VQU28_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002202361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476726.1"
                     /translation="MIAHTIHQKFNLKVPNYRQEEDWARMGLPIIRKEISNWHIKTSQ
                     YYLEPLYNLLRERLLTQPLLHADETSYRVLESDSQLTYYWTFLSGKAEKQGITLYHHV
                     LIDLFISYFNPL"
     gene            complement(581..1501)
                     /gene="cysK"
                     /locus_tag="VQU28_001381"
     CDS             complement(581..1501)
                     /gene="cysK"
                     /locus_tag="VQU28_001381"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HET2476727.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKLGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(1786..2421)
                     /locus_tag="VQU28_001382"
     CDS             complement(1786..2421)
                     /locus_tag="VQU28_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HET2476728.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVSVNLV"
     gene            2477..3775
                     /locus_tag="VQU28_001383"
     CDS             2477..3775
                     /locus_tag="VQU28_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2476729.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTL
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            3772..4434
                     /locus_tag="VQU28_001384"
     CDS             3772..4434
                     /locus_tag="VQU28_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HET2476730.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQACQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            4514..5062
                     /gene="raiA"
                     /locus_tag="VQU28_001385"
     CDS             4514..5062
                     /gene="raiA"
                     /locus_tag="VQU28_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HET2476731.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            5198..7171
                     /locus_tag="VQU28_001386"
     CDS             5198..7171
                     /locus_tag="VQU28_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HET2476732.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDALGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNTVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            7168..7620
                     /gene="rplI"
                     /locus_tag="VQU28_001387"
     CDS             7168..7620
                     /gene="rplI"
                     /locus_tag="VQU28_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HET2476733.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            7664..9016
                     /gene="dnaB"
                     /locus_tag="VQU28_001388"
     CDS             7664..9016
                     /gene="dnaB"
                     /locus_tag="VQU28_001388"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HET2476734.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            9018..9284
                     /locus_tag="VQU28_001389"
     CDS             9018..9284
                     /locus_tag="VQU28_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476735.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            9373..10719
                     /gene="cbpD"
                     /locus_tag="VQU28_001390"
     CDS             9373..10719
                     /gene="cbpD"
                     /locus_tag="VQU28_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HET2476736.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSRG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGGTHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      10802..10904
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            10975..11265
                     /locus_tag="VQU28_001391"
     CDS             10975..11265
                     /locus_tag="VQU28_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HET2476737.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            11228..11986
                     /locus_tag="VQU28_001392"
     CDS             11228..11986
                     /locus_tag="VQU28_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476738.1"
                     /translation="MRNLKSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     NPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRT"
     gene            12024..13031
                     /locus_tag="VQU28_001393"
     CDS             12024..13031
                     /locus_tag="VQU28_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2476739.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            13031..13759
                     /locus_tag="VQU28_001394"
     CDS             13031..13759
                     /locus_tag="VQU28_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476740.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            13878..14336
                     /locus_tag="VQU28_001395"
     CDS             13878..14336
                     /locus_tag="VQU28_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HET2476741.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            14338..16770
                     /locus_tag="VQU28_001396"
     CDS             14338..16770
                     /locus_tag="VQU28_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HET2476742.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFMELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALVLGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     ALRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKVKYVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            17412..18065
                     /gene="rr06"
                     /locus_tag="VQU28_001397"
     CDS             17412..18065
                     /gene="rr06"
                     /locus_tag="VQU28_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HET2476743.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDIWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            18062..19402
                     /gene="hk06"
                     /locus_tag="VQU28_001398"
     CDS             18062..19402
                     /gene="hk06"
                     /locus_tag="VQU28_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HET2476744.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILFLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            19485..20012
                     /locus_tag="VQU28_001399"
     CDS             19485..20012
                     /locus_tag="VQU28_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HET2476745.1"
                     /translation="MVLAIILVTFFIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            20091..>21310
                     /locus_tag="VQU28_001400"
     CDS             20091..>21310
                     /locus_tag="VQU28_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HET2476746.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLFLGGVVHAEGVRSGD
                     TPKVTSSGDEVESHLKKILSEIQTQLDRKRHAKTVALINKLQDIKRTYSYKLNVLEEK
                     PKAELTSKTKEELTAAFEQFKKDTLPTEPGKKVAEAEKKVAEAEKKAEDQKEEDRRNY
                     PTNTYKTLELEIAEFDVKVKEAELELLKEKAKKPLNEGTIKQAKEKVESKKAEATRLE
                     NIKTDRKKAEEEAKRKAAEEDKVKEKPAEQPQPAPAPQPEKPAPKPEKPAPAPKPENP
                     APKPEKPAPAPKPENPAEQPKAEKPADQQAEEDYARRSEEEYNRLTQQQPPKTEKPAQ
                     PSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYL
                     NANGSMATGWLQNNG"
BASE COUNT         6688 a         3546 c         4808 g         6268 t
ORIGIN      
        1 cctaaagccc ctttagcgca taactttggc tcagcttcta ttatcgctca caccatccat
       61 cagaagttta atctgaaggt acccaattat cgccaagaag aagattgggc taggatgggt
      121 ttaccaatca tacgtaagga aatctctaat tggcatatca agacgagtca atactatttg
      181 gagccccttt ataacctctt gcgagagaga ctattgactc agcccttact tcatgcggat
      241 gaaacttctt atagggtgct agagagtgat agtcagctga cttactactg gacttttttg
      301 tctgggaaag ctgagaaaca agggattacg ctttaccatc atgtcctaat cgatttgttc
      361 atatcttatt tcaatccatt ataaatagtg agaaatatct atcctatctt ctagaatgtc
      421 ttccaaacgt ggaaactctc gtaaacaaag aggttttaga ggcctattta ccatggacta
      481 aagttgtaca agaaaagtgc aaataagaaa tctccagatt aggaactatc cgtgagttct
      541 ctagtctgga gatttttcaa tagacttcgt tattggacgg ttacaattca taaagtgctg
      601 tagagagata acgttcaccg ttatctggtg ctagggcaag gactttttta cctgtaccta
      661 attttttggc aacctcgatg gctccgtaga tagctgcagc tgaggaaatc cctacaagga
      721 agccttcttt tccaccaatt tcacgtccga gtgcaagagc gtcatctgat gttacacgaa
      781 cgataccatc ataggcttta gtatcaagtg tatcaggaat aaatccagct gagatacctt
      841 gaattttgtg aggaccaggt ttttcaccag atagaatagc agattcatct gcttctactg
      901 caaaaacttg aatgttagaa ttttctgatt tgagtgcatg agaaacacca gaaatcgttc
      961 caccagtacc tactccagca acaaaggcat ctaatccatc tttaccgaaa gcagctagta
     1021 tctcagctcc tgttgttctt tcgtgtactt ctggattagc tggattgtca aattgaagag
     1081 gaaggaaacc atcacgttca gcagcgattt cttgagcctt agcaatagca cctttcattc
     1141 cctcgctacc aggagttagg acgagttcag caccataagc ttggataatt ttacgtcgtt
     1201 ctacactcat agtttcaggc ataacgatga cgactttata ccctttagca gcacctaccc
     1261 atgaaagtcc aataccggtg tttccacttg ttgcttcaac aatagtagaa ccaagtttca
     1321 gaataccatc ttgttcagct ttttcaatca tgctaagggc aatacggtct tttacagatg
     1381 aaccaggatt aaatgcttca agctttatat agacgtctgc agcaccttct ggcacgatgt
     1441 tgttaagttt aacaatcggt gtttgaccga ttaattcagt aatgttgtta taaatagaca
     1501 tatgaatacc tctttgtata agatatcttt agtataacgc tatccatacc atttgtaaaa
     1561 tatagaaatc ctatcgaagc gataggattt ctatatatca tagatcaaag tcattaattt
     1621 tcaagtgatt gcgttcagtt aattctagta aataggtgtg aaggaaaacg atatcaggtt
     1681 tcgtttgaat tgcttcattt tctagtatat tttggtacgc atctttttat tttgctcgct
     1741 aaagtagttg tgagaatgtc aacgaaacgc tgtattattc attgtttaca ctaagtttac
     1801 agaaacttca acctctcgta aaccttggtc agttaaagtg acttttccat taaaaaactc
     1861 cacaagtgca gctttaatag tttctttttc ttctttatca acataaatca tcgtatcgac
     1921 ttgatctgta aagtttgtat ccagctccat gagatcatgt tctttaagga agttactgta
     1981 ctcttggtac tgagcataag acatttgaat agcaatgcca gcctgttctt ttatttcaat
     2041 aataccaatt tctttgacag ctaaggcgac actgccggcg taagcacgaa ttagtcctcc
     2101 agcgcctagt ttaataccac caaagtagcg tgtcacgacc acacagacat tggtgagatt
     2161 gtgattttct agtaccccaa gcatgggaac accagcagta ccactaggct caccatcatc
     2221 acttgtacgt ttaatttcac tacgttctcc aataataaag gcagagcagt tatgtgtcgc
     2281 tttgtagtgt tcttttttga tggcagtaat gaagtcacga gcctcttctt cgctataaac
     2341 acgcttggca tggcagataa agcgagattt tttgatttct tcttggactt ggccgtcctc
     2401 tttaattgtc ctaaattcca tgatttaatt gtactaaaaa ataatctaaa gtgctagttt
     2461 ttacgaataa agaagtatga aagtaaattt agattatctc ggtcgtttat ttactgagaa
     2521 tgaattaaca gaagaagaac gtcagttggc ggagaaactt ccagcaatga gaaaggagaa
     2581 ggggaaactt ttctgtcaac gttgtaatag tactattcta gaagaatggt atttgcccat
     2641 cggtgcttac tattgtcgag agtgcttgct gatgaagcga gtcagaagtg atcaaacttt
     2701 atactatttt ccgcaggagg attttccgaa gcaagatgtt ctcaaatggc gcggccaatt
     2761 aactcctttt caagagaagg tgtcagaggg actgcttcaa gcagtagaca agcaaaagcc
     2821 aaccttagtt catgcggtaa caggagctgg aaagacagaa atgatttatc aagtagtggc
     2881 taaagtgatc aatgcgggtg gtgcagtgtg tttggctagt cctcgcatag atgtttgttt
     2941 ggagctgtac aagcgcctgc aacaggattt ttcttgcggt atagctttgc tacatggaga
     3001 atcggaacct tattttcgaa caccactagt tgttgcaaca acccatcagt tattgaagtt
     3061 ttatcaagct tttgatttgc tgatagtgga tgaagtagat gcttttcctt atgttgataa
     3121 tcccacgctt taccacgctg tcaagaatag tgtaaaggag aatggattga gaatcttttt
     3181 aacagcgact tcgaccaatg agttagataa aaaggtccgt ttaggagaac taaaaagact
     3241 gagtttaccg agacggtttc atggaaatcc gttgattatt ccaaaaccaa tttggttatc
     3301 ggattttaat cgctacttag acaagaatcg tttgtcacca aagttaaagt cctatattga
     3361 gaagcagaga aagacagctt atccgttact catttttgct tcagaaatta agaaagggga
     3421 gcagttagaa gaaatcttac aggagcaatt tccaaatgag aaaattggct ttgtatcttc
     3481 tgtaacagag gatcgattag agcaagtaca agcttttcga gatggagaac tgacaatact
     3541 tatcagtacg acaatcttgg agcgtggagt taccttccct tgtgtggatg ttttcgtaat
     3601 agaggccaat catcgtttgt ttaccaagtc tagtttgatt cagattggtg gacgagttgg
     3661 acgaagcatg gatagaccga caggagattt gcttttcttc catgatgggt taaatgcttc
     3721 aatcaagaag gcgattaagg aaattcagat gatgaataag gaggctggtc tatgaagtgc
     3781 ttgttatgtg ggcagactat gaagactgtt ttaactttta gtagtctctt acttctgagg
     3841 aatgatgact cttgtctttg ttcagactgt gattctactt ttgaaagaat tggggaagag
     3901 aactgtccaa attgtatgaa aacagagttg tcaacaaagt gtcaagcttg tcaattttgg
     3961 tgtaaagaag gagttgaagt cagtcataga gcgattttta cttacaatca agctatgaag
     4021 gattttttca gtcggtataa gtttgatgga gacttcctgt taagaaaagt tttcgcttca
     4081 tttttaagtg aggagttgaa aaagtacaaa gagtatcaat ttgttgtaat tcccctaagt
     4141 cctgatagat atgctaacag aggatttaat caggttgagg gcttggtaga ggcagcaggc
     4201 tttgagtatc tagatttatt agagaaaaga gaagagagag ccagttcttc taaaaatcgt
     4261 tcagagcgct tggggacaga acttcctttc tttattaaaa gtggagtcac tattcctaaa
     4321 aaaatcctac ttatagatga tatctatact acaggagcaa ctataaatcg tgttaagaaa
     4381 ctgttggaag aagctggtgc taaggatgta aaaacatttt cccttgtaag atgaggaaaa
     4441 aatttgcaaa aatagaatga aagcgttata ataaaatcat aaaaacaaaa atgtttagaa
     4501 agaaggtact catatgatta aatatagtat ccgtggtgaa aacctagaag taacagaagc
     4561 aattcgtgat tatgtagttt ctaaactcga aaagatcgaa aagtacttcc aaccagaaca
     4621 agagttggat gcccgaatta acttaaaagt ttatcgtgaa aaaacggcta aagtggaagt
     4681 aacgattccg cttggatcta ttactctccg cgcagaagat gtatctcaag atatgtatgg
     4741 ttcaattgac cttgtaactg ataaaattga acgtcagatt cgtaaaaata aaacaaaaat
     4801 cgagcgtaaa aataaaaata aggtagcaac tggtcaatta tttacagatg ctttggtgga
     4861 agattcaaat attgtccagt ctaaagttgt tcgttcaaaa caaattgatt taaaaccaat
     4921 ggatttggaa gaagcaattc tacaaatgga tttattgggg catgatttct ttatctatgt
     4981 ggatgttgaa gatcagacaa ccaatgtgat ttatcgtcgt gaggatggcg aaattggttt
     5041 gttagaggtt aaagaatctt aatttcaata tgtgtaaagg taggtttact gaattgtaaa
     5101 ctaccttttt cttataattg atagaattac tgattacact tttaaaaagt cgctttttga
     5161 ggggtattat ggtataatgg gtgccaagag gttttgaatg aaaaaatttt atgtaagtcc
     5221 aatttttcct attctagtag gattgattgc gtttggagtc ttatccactt tcattatttt
     5281 tgttaataat aatctgttga cggttttaat tttgtttctt tttgtaggag gctatgtttt
     5341 tttatttaag aaactgagag tgcattatac aaggagtgat gtagaacaga tacagtatgt
     5401 aaaccaccaa gcggaagaaa gtttgacagc tctattggaa cagatgcctg taggtgttat
     5461 gaaattgaat ttatcttctg gagaggttga gtggtttaat ccctatgctg aattgatttt
     5521 gaccaaggaa gatggtgatt ttgatttaga agctgttcaa acgattatca aggcttcagt
     5581 aggaaatccg tctacttatg ccaagcttgg tgagaagcgt tatgctgttc atatggatgc
     5641 ttcttccggt gttttgtatt ttgtagatgt atccagggaa caagccataa cagatgaatt
     5701 ggtaacaagt cgaccagtga ttgggattgt ctctgtggat aattatgatg atttggagga
     5761 tgcaacttct gagtcagata ttagtcaaat caatagtttt gtagctaatt ttatatcaga
     5821 gttttcagaa aaatacatga tgttttctcg tcgggtaagt atggatcgat tttatctatt
     5881 tactgactac acggtgcttg agggcttgat gaatgataaa ttttctgtta ttgatgcttt
     5941 cagagaagag tcgaaacaga gacagttgcc cttgacctta agtatgggat tttcttatgg
     6001 cgatggaaat catgatgaga tagggaaagt tgctttgctc aatttgaact tggctgaagt
     6061 acgtggtggc gaccaggtgg ttgttaagga aaacgacgaa acgaaaaatc cagtttattt
     6121 tggtggtggg tctgctgctt caatcaagcg tacacggact cgtacgcgcg ctatgatgac
     6181 agctatttca gataagattc ggagtgtaga tcaggttttt gtagtcggtc acaaaaattt
     6241 agacatggat gctttgggct ctgctgtagg tatgcagttg ttcgccagca atgtgattga
     6301 aaatagctat gctctttatg atgaagaaca aatgtctcca gatattgaac gagctgtttc
     6361 attcatagaa aaagaaggag ttacgaagtt gttgtctgtt aaggatgcat tggggatggt
     6421 gaccaatcgt tctttgttga ttcttgtaga ccattcaaag acagccttaa cattatcaaa
     6481 agaattttat gatttattta cccaaaccat tgttattgac caccatagaa gggatcagga
     6541 ttttccagat aatacggtca ttacttatat cgaaagtggt gcaagtagtg ccagtgagtt
     6601 ggtaacggaa ttgattcagt tccagaattc taagaaaaat cgtttgagtc gtatgcaagc
     6661 aagtgtcttg atggctggta tgatgttgga tactaaaaat ttcacctcgc gagtaactag
     6721 tcggacattt gatgttgcta gctatctcag aacgcgcgga agtgatagta ttgctatcca
     6781 ggaaatcgct gcgacagatt ttgaagaata tcgtgaggtc aatgaactta ttttacaggg
     6841 gcgtaaatta ggttcagatg tactaatagc agaggctaag gacacgaaat gctatgatac
     6901 agttgttatt agtaaggcag cagatgccat gttagccatg tcaggtattg aagcgagttt
     6961 tgttcttgcg aagaatacac aaggatttat ctctatctca gctcgaagtc gtagtaaact
     7021 gaatgtacaa cggattatgg aagagttagg cggtggaggc cactttaatt tggcagcagc
     7081 tcaaattaaa gatgtaacct tgtcagaagc aggtgaaaaa ctgacagaaa ttgtattaaa
     7141 tgaaataaag gaaaaggaga aagaagaatg aaagtaatct ttttagcaga tgttaaagga
     7201 aaaggtaaaa aaggcgaaat taaggaagta ccaacagggt atgcgcaaaa ctttcttatc
     7261 aaaaagaatc tagccaaaga agcgactgct caagctgtag gtgaacttcg tggtaaacaa
     7321 aaatcggaag aaaaagctca cgctgagatg attgcagaag gaaaagcaat taaagcacaa
     7381 cttgaagcag aagaaactgt tgtagaattt gttgaaaaag ttggtccaga tggtcgtacc
     7441 tttggctcta ttaccaataa gaagattgca gaagaattgc aaaaacaatt tggaattaag
     7501 attgataaac gtcatattca agtacaagct ccgattcgag cggttggttt gattgatgtg
     7561 ccagtgaaaa tctatcaaga tatcacaagt gtaatcaatc ttcgtgtgaa agaaggataa
     7621 gtttacacct tcttgacaag attgtaaaag gaagggaagt ctgatggcag aagtagaaga
     7681 gttacgagta caacctcaag atatcttagc tgagcaatcc gttttagggg ctatctttat
     7741 tgatgagagt aaacttgttt ttgtgcgaga atacattgag tctcgggact tttttaagta
     7801 tgcccatcgt ttgattttcc aagccatggt cgatttatcc gatcgtggtg atgccataga
     7861 tgcaacaacg gttcgtacta tccttgataa tcaaggtgat ttacagaata ttggtggctt
     7921 gtcttacttg gttgagattg ttaattctgt gccaacttct gctaatgcgg agtattatgc
     7981 taagattgtt gcagaaaaag caatgctacg tcgtttaatt gccaagttga cagagtctgt
     8041 caaccaagct tacgaagcgt cacaaccagc tgatgaaatt attgctcagg cagaaaaagg
     8101 gctgattgat gtcagtgaaa atgcaaatcg aagcgggttt aagaacattc gagatgtgtt
     8161 gaatctcaac tttggaaatc tggaagctcg ctcgcaacaa acgaccgata ttacaggtat
     8221 tgcgacaggt tatcgtgatt tggatcatat gacaacagga cttcatgagg aggagttgat
     8281 tatcttagca gctcgtccag cagttggtaa gacagcattt gccttgaata tcgctcagaa
     8341 cattgggact aagttggaca aaacggttgc tattttttca ctcgaaatgg gtgcggaaag
     8401 cttggtagat cgtatgttag ctgcagaagg cttagtggag tcacattcta tccgtacagg
     8461 gcaattgaca gatgaggagt ggcaaaaata tactattgct cagggtaatc tagctaacgc
     8521 cagtatctat atcgatgata cgccaggtat tcggattaca gagattcgtt ctcgttctcg
     8581 taaattggct caagaaactg gaaatcttgg tttgattttg atagactatt tgcaacttat
     8641 cacgggaact ggtcgagaaa atcgtcaaca agaagtttct gaaatttctc gtcagttgaa
     8701 aatactagcc aaggaattga aggttccagt aatcgctctg agtcagcttt ctcgtggtgt
     8761 agaacaacgt caggacaaga gaccggtctt gtctgatatt cgtgaatctg ggtctattga
     8821 gcaggacgct gatatcgtag cttttctcta tcgcgatgac tactatgaac gtggtggtga
     8881 agaagaggag ggtatcccaa ataataaggt ggaagttatt atcgagaaaa accgtagtgg
     8941 agctcgtgga acagtggaat tgattttcca aaaagaatac aataaatttt caagtatctc
     9001 aaagagggag gcataagatg tcagatgcat ttacagatgt agccaagatg aaaaaaatca
     9061 aagaagaaat caaggcacat gagggacaag tcgtagaaat gactttggag aatggtcgta
     9121 agcgccaaaa aaatagattg ggtaagctaa ttgaagttta tccatctcta tttattgtgg
     9181 agtttgggga tgtggaagga gataaacaag ttaatgttta cgttgaatcc tttacttact
     9241 cagatattct tacagaaaag aatttgattc attatcttga ctaaagtgag aaattttctc
     9301 actttttctt ttttctccga ataatttagg tgaaggcaat catcgcttta tattattttt
     9361 caaggaggaa gaatgaaaat tttaccgttt atagcaagag gaacaagtta ttacttgaag
     9421 atgtcagtta aaaagcttgt tcctttttta gtagtaggat tgatgctagc agctggtgat
     9481 agtgtctatg cctattccag aggaaatgga tcgattgcgc gtggggatga ttatcctgct
     9541 tattataaaa atgggagcca ggagattgat cagtggcgca tgtattctcg tcagtgtact
     9601 tcttttgtag cctttcgttt gagtaatgtc aatggttttg aaattccggc agcttatgga
     9661 aatgcgaatg aatggggaca tcgtgctcgt cgggaaggtt atcgtgtaga taatacaccg
     9721 acgattggtt ccattacttg gtctactgca ggaacttatg gtcatgttgc ctgggtgtca
     9781 aatgtaatgg gagatcagat tgagattgag gaatataact atggttatac agaatcctat
     9841 aataaacgag ttataaaagc aaacacgatg acaggattta ttcattttaa agatttggat
     9901 ggtggcagtg ttgggaatag tcaatcctca acttcaacag gcggaactca ttattttaag
     9961 accaagtctg ctattaaaac tgaaccccta gttagtgcaa ctgtgattga ttactattat
    10021 cctggagaga aggttcatta tgatcaaatt ctcgaaaaag acggctacaa gtggttgagt
    10081 tatactgcct ataatggaag ctatcgttat gttcaattgg aggctgtgaa taaaaatcct
    10141 ctaggtaatt ctgttctttc ttcaacaggt ggaactcatt attttaagac caagtctgct
    10201 atcaaaactg aacccctagt tagcggaact gtgattgatt actattatcc tggggagaag
    10261 gttcattatg atcagatact tgaaaaagac ggctacaagt ggttgagtta tactgcctat
    10321 aatggaagtc gtcgctatat acagctagag ggagtgactt cttcacaaaa ttatcagaat
    10381 caatcaggaa atatctctag ctatggatcc aataatagtt caactgtcgg ttggaagaaa
    10441 ataaatggta gttggtatca tttcaaatca aatggttcta aatcaacagg atggctgaaa
    10501 gacggttcta gctggtatta tttgaaatta tctggtgaaa tgcagacagg atggttaaag
    10561 gagaatggct cgtggtatta tctgggtagt tcaggggcaa tgaaaacagg ctggtaccag
    10621 gtctctggta agtggtatta ttcttactct tcaggcgcct tagctgttaa tacgacggtg
    10681 gatggctaca gagtaaacag tgatggagaa cgagtataga aaattggagt aggagaaatt
    10741 tcctgctctt ttctgtaagc agtttccttg acatttttct tattttgcgc tatcatatat
    10801 acatataata tatgggagtc tgtgtacagt ctgagaggaa gtgttaaact tcgaccgcac
    10861 ctgatctggg taatgccagc ggagggaacg atacttagtc taattttgca ccttttccat
    10921 gtatggtaaa ggtttttctt ttttttaaaa ggaaaacgag aagaggaggt tcttatgaaa
    10981 gcaagcattg ccttgcaagt tttaccccta gcacagggga ttgatcggat agctgttatt
    11041 gatcaggtca ttgcttatct gcaaactcaa gaagtgacga tggtagtgac accatttgaa
    11101 acggtcttgg aaggggagtt tgatgagctt atgcgcattc taaaagaagc gctggaagtg
    11161 gcagggcagg aggcagacaa tgtctttgcc aatgtcaaaa taaatgtagg agagatttta
    11221 agtattgatg agaaacttga aaagtatact gagacgacac attagtctat tgggctttct
    11281 cggagtattg tcaatctggc agttagcagg ttttcttaaa cttctcccca agtttatcct
    11341 gccgacacct cttgaaattc tccagccctt tgttcgtgac agagaatttc tctggcacca
    11401 tagctgggcg accttgagag tggctttact ggggctgatt ttgggagttt tgattgcctg
    11461 tcttatggct gtgctcatgg atagtttgac ttggctcaat gacctgattt accctatgat
    11521 ggtggtcatt cagaccattc cgaccattgc catagctcct atcctggtct tgtggctggg
    11581 ttatgggatt ttgcccaaga ttgtcttgat tatcttaaca acaacctttc ccatcatcgt
    11641 tagtattttg gacggtttta ggcattgtga caaggatatg ctgactttgt ttagtctgat
    11701 gcgagcaaac ccttggcaaa tcctgtggca ttttaaaatc ccggttagcc tgccttactt
    11761 ttatgcaggt ctgagggtca gtgtctccta cgcctttatc acaactgtgg tatctgagtg
    11821 gttgggaggt tttgaaggtc ttggtgttta tatgattcag tctaaaaaac tgtttcagta
    11881 tgataccatg tttgccatta ttattctggt gtcgattatc agtcttttgg gtatgaagct
    11941 ggtcgatatc agtgaaaaat atgtgattaa atggaaacgt acttaaaata gagcgttttt
    12001 gaaaaagaaa agaggaaatc aaaatgaaga aaacatggaa agtgttttta acgcttgtaa
    12061 cagctcttgt agctgttgtg cttgtggcct gtggtcaagg aactgcttct aaagacaaca
    12121 aagaggcaga acttaagaag gttgacttta tcctagactg gacaccaaat accaaccaca
    12181 cagggcttta tgttgccaag gaaaaaggtt atttcaaaga agctggagtg gatgttgatt
    12241 tgaaattgcc accagaagaa agttcttctg acttggttat caacggaaag gcaccatttg
    12301 cagtgtattt ccaagactac atggctaaga aattggaaaa aggagcagga atcactgccg
    12361 ttgcagctat tgttgaacac aatacatcag gaatcatctc tcgtaaatct gataatgtaa
    12421 gcagtccaaa agacttggtt ggtaagaaat atgggacatg gaatgaccca actgaacttg
    12481 ctatgttgaa aaccttggta gaatctcaag gtggagactt tgagaaggtt gaaaaagtac
    12541 caaataacga ctcaaactca atcacaccga ttgccaatgg cgtctttgat actgcttgga
    12601 tttactacgg ttgggatggt atccttgcta aatctcaagg tgtagatgct aacttcatgt
    12661 acttgaaaga ctatgtcaag gagtttgact actattcacc agttatcatc gcaaacaacg
    12721 actatctgaa agataacaaa gaagaagctc gcaaagtcat ccaagccatc aaaaaaggct
    12781 accaatatgc catggaacat ccagaagaag ctgcagatat tctcatcaag aatgcacctg
    12841 aactcaagga aaaacgtgac tttgtcatcg aatctcaaaa atacttgtca aaagaatacg
    12901 caagcgacaa ggaaaaatgg ggtcaatttg acgcagctcg ctggaatgct ttctataaat
    12961 gggataaaga aaatggtatc cttaaagaag acttgacaga caaaggcttc accaacgaat
    13021 ttgtgaaata atgacagaaa ttagactaga gcatgtcagt tatgcctatg gtcaggagag
    13081 gattttagag gatatcaacc tacaggtgac ttcaggcgaa gtggtttcca ttctaggccc
    13141 aagtggtgtt ggaaagacca ccctctttaa tctaatcgct gggattttag aagttcagtc
    13201 agggagaatt gtccttgatg gtgaagaaaa tcccaagggg cgcgtgagtt atatgttgca
    13261 aaaggatctg ctcttggagc acaagacggt gcttggaaat atcattctgc ccctcttgat
    13321 tcaaaaggtg gataaggcag aagctatttc ccgagcggat aaaattcttg cgaccttcca
    13381 gctgacagct gtaagagaca agtatcctca tgaacttagc ggtgggatgc gccagcgtgt
    13441 agccttactc cggacctacc tttttgggca caagctcttt ctcttagatg aggcctttag
    13501 cgccttggat gagatgacaa agatggaact ccacgcttgg tatcttgaga ttcacaaaca
    13561 gttgcagcta acaaccctga tcatcacgca tagtattgag gaggccctca atctcagcga
    13621 ccgtatctat atcttgaaaa atcgccctgg gcagattgtt tcagaaatta aactagattg
    13681 gtctgaagat gaggacaagg aagtccaaaa gattgcctac aaacgtcaaa tcttggcaga
    13741 attaggctta gataagtaga aaaataggga gttggtgaag attatccttt accagcgccc
    13801 tttttctttt aaaaatgaga aaatttcggt ataatagtca aacaaggtca aggtttaaag
    13861 agagaggtgg gtttgttatg agatttaaaa atacatcgga tcatattgag gcctacatca
    13921 aggcgatttt agatcaatct ggtatcgtgg agttgcaacg gagtcagttg gcagatacct
    13981 ttcaggttgt tcctagtcag attaactacg tgatcaagac acgctttacg gaaagtagag
    14041 gctacttggt tgaaagtaag cgtggtggcg gaggctacat tcgtatagga cggattgagt
    14101 tttctagtca tcatgaaatg ctccgggagc tgctttactc gattggtgag cgagtcagtc
    14161 aagaaattta tgaggatatt ctccagcttt tggttgagca ggaattgatg accaagcagg
    14221 agatgaattt gctagaatca gtagctttgg atcgcgtttt aggagaagaa gctccagttg
    14281 ttcgagcaaa catgctacgt caggtcatac aagaggtaga tagaaaaggg aagtaagatg
    14341 aactattcaa aagcattgaa tgaatgtatc gaaagtgcct acatggttgc tggacatttt
    14401 ggagctcgtt atctagagtc gtggcacttg ttgattgcca tgtctaatca cagttatagt
    14461 gtagcagggg caactttaaa tgattatccg tatgagatgg accgtttaga agaggtggct
    14521 ttggaactga ctgaaacgga ctatagccag gatgaaacct ttatggaatt gccgttctcc
    14581 cgtcgtttgc aggttctttt tgatgaagca gagtatgtag cgtcagtggt ccatgctaag
    14641 gtactaggga cagagcacgt cctctatgcg attttgcatg atggcaatgc cttggcgact
    14701 cgtatcttgg agagggctgg tttttcttat gaagacaaga aagatcaggt caagattgct
    14761 gctcttcgtc gaaatttaga agaacgggca ggctggactc gtgaagatct caaggcttta
    14821 cgccaacgcc atcgtactgt ggctgacaaa caaaattcta tggccaatat gatgggcatg
    14881 ccgcagactc ctagtggtgg tctcgaggac tatacgcatg atttgacaga gcaagcgcgt
    14941 tctggcaagt tagaaccagt catcggtcgg gacaaggaaa tctcacgtat gattcaaatc
    15001 ttgagccgta agactaagaa caaccctgtc ttggttgggg atgctggtgt cgggaaaaca
    15061 gctctggtgc ttggtcttgc tcagcgtatt gccagtggtg atgtgcctgc ggaaatggct
    15121 aagatgcgcg tgttagaact tgatttgatg aatgtcgttg cagggacacg cttccgtggt
    15181 gactttgaag aacgcatgaa taatatcatc aaggatattg aagaagatgg ccaagtcatc
    15241 ctctttatcg atgaactcca caccatcatg ggttctggta gcgggattga ttcgactctg
    15301 gatgcggcca atatcttgaa accagccttg gcgcgtggag ctttgagaac ggttggtgcc
    15361 accactcagg aagaatatca aaaacatatc gaaaaagatg cggctctttc tcgtcgtttc
    15421 gctaaagtga cgattgaaga accaagtgtg gcagatagta tgactatttt acaaggtttg
    15481 aaggcgactt atgagaaaca tcaccgtgta caaatcacag atgaagcggt tgaaacagcg
    15541 gttaagatgg ctcatcgtta tttaaccagt cgtcacttgc cagactctgc tatcgacctc
    15601 ttggacgaag cagcagcaac agtgcaaaat aaggtaaagt atgtaaaagc agacgattca
    15661 gatttgagtc cagctgataa ggccctgatg gatggcaagt ggaaacaggc agcccagcta
    15721 atcgcaaaag aagaggaagt acctgtctac aaagacttgg tgacagagtc tgatattttg
    15781 accaccttga gtcgcttgtc aggaatccca gttcaaaaac tgactcaaac ggatgctaag
    15841 aagtatctaa atcttgaagc agaactccat aaacgtgtca tcggtcaaga tcaagctgtt
    15901 tcaagcatta gtcgtgcgat tcgccgtaat cagtcaggaa ttcgcagtca caagcgtccg
    15961 atcggttcct ttatgttcct aggacccaca ggtgtcggta agaccgagtt ggccaaggct
    16021 ttggcagaag ttctctttga tgacgaatca gcccttatcc gctttgatat gagtgagtat
    16081 atggagaaat tcgcagccag ccgtctaaat ggagctcctc caggctatgt gggttacgaa
    16141 gaaggtggag agttgacaga gaaggttcgc aataaaccct attccgttct cctctttgat
    16201 gaggtagaga aggcccaccc agatattttt aatgttctct tgcaggttct ggatgacggt
    16261 gttttgacag atagcaaggg acgcaaggtc gatttttcaa ataccattat cattatgaca
    16321 tcgaacctag gtgcgactgc ccttcgtgat gataagactg ttggttttgg ggctaaggat
    16381 attcgttttg accaggaaaa tatggaaaaa cgcatgtttg aagaactgaa aaaagcttat
    16441 agaccggaat tcatcaaccg tattgatgag aaggtggtct tccatagcct atctagtgac
    16501 catatgcagg aagtggtgaa gattatggtc aagcctttag tggcaagttt ggctgaaaaa
    16561 ggcattgact tgaaattaca agcttcagct ctgaaattgt tagcaaatca aggatatgac
    16621 ccagagatgg gagctcgccc acttcgcaga accctgcaaa cagaagtgga ggacaagttg
    16681 gcagaacttc ttcttaaggg agatttagtg gcaggcagca cacttaagat tggtgtcaaa
    16741 gcaggccagt taaaatttga tattgcataa aagaataaaa gtatcagcat ctgaccataa
    16801 gtcacagtgg agtgaaactc aatgaaaatc aaagagcaaa ctaggcagct agccgcaggt
    16861 tgctcaaaac actggtttga ggttgcagat agagctgacg tggtttgaag agattttcga
    16921 agagtatgaa actaaaacct atagcttcta aacgatccgt ggttttcacc attcaacaca
    16981 aaattcatat gtttattacc ctccgtcgta tttgtcttag agcgtgtgta gtagaaaaag
    17041 agcagtctta tctgaaattt ttattctttc aaaagagacc tgtttctttt ttgcatgtca
    17101 aatccgttct agctggtatt tgaaaaatca aactaatatt caatgaaaat caaagaacaa
    17161 actaggcagc tagccacagg ttgctcaaaa cactgttttg aggttgtaga tagagctgac
    17221 gtagtttgaa gagattttcg aagagtataa gctgcaagat gaatgatttt cttgtattga
    17281 cgttgttgtt gacaaaaagt agcggataaa tgaaatccat tccattatca tagatgatag
    17341 gctggtagga aattttcaaa tagcatacag gaaatagatg tatggagttc tggtagtaga
    17401 aagggagaga gatgaacatt ttagttgcag atgacgagga aatgattaga gaaggaattg
    17461 cagcatttct gacagaagag ggttatcatg tcattatggc taaggatgga caagaggtct
    17521 tggaaaaatt tcaagatctc cctatccatc tcatggtatt ggatttaatg atgcctagga
    17581 agagtggttt tgaagtgtta aaagaaatca atcaaaagca cgatattcct gtcatcgtct
    17641 tgagtgctct gggagatgaa actactcagt cacaggtatt tgatctctat gctgatgatc
    17701 atgtgacaaa acctttttct ttggtactgc ttgtcaagcg tattaaggcg cttatcagac
    17761 gttactacgt catagaggat atttggcgat atcaggatgt aacagtggat tttacctctt
    17821 acaaagcaca ttataaaaat gaagaaattg atctcaaacc aaaggaatta ctggtactaa
    17881 agtgtttgat tcagcataaa aatcaagttt taagtagaga gcagatattg gaagaaattt
    17941 caaaagatgt agctgattta ccttgtgata gggtcgttga tgtctatatt cgtactcttc
    18001 gcaaaaaatt agctttagat tgtatcgtga ctgtgaaaaa tgttgggtat aagattagct
    18061 tatgataaaa aatcctaaat tattaaccaa gtctttttta agaagttttg caattctagg
    18121 tggtgttggt ctagtcattc atatagctat ttatttgacc tttccttttt attatattca
    18181 actggagggg gaaaagttta atgagagcgc aagagtgttt acggagtatt taaagactaa
    18241 gacatctgat gaaattccaa gcttactcca gtcttattca aagtccttga ccatatctgc
    18301 tcaccttaaa agagatattg tagataagcg actccctctt gtgcatgact tggatattaa
    18361 agatggaaag ctatcaaatt atatcgtgat gttagatatg tctgttagta cagcagatgg
    18421 taaacaggta acagtgcaat ttgttcacgg ggtggatgtc tacaaagaag caaagaatat
    18481 tttgtttttg tatcttccat atacattttt ggttacaatt gctttttcct ttgttttttc
    18541 ttatttttat actaaacgct tgctcaatcc tcttttttac atttcagaag tgactagtaa
    18601 aatgcaagat ttggatgaca atattcgttt tgatgaaagt aggaaagatg aagttggtga
    18661 agttggagaa gttggaaaac agattaatgg tatgtatgag cacttgttga aggttattca
    18721 tgagttggaa agtcgtaatg agcaaattgt aaaattgcaa aatcaaaagg tttcctttgt
    18781 ccgtggagca tcacatgagt tgaaaacccc tttagccagt cttagaatta tcctagagaa
    18841 tatgcagcat aatattggag attacaaaga tcatccaaaa tatattgcaa agagtataaa
    18901 taagattgac cagatgagcc acttattgga agaagtactg gagtcttcta aattccaaga
    18961 gtggacagag tgtcgtgaga ccttgactgt taagccagtt ttagtagata ttttatcacg
    19021 ttatcaagaa ttagctcatt caataggtgt tacaattgaa aatcaattga cagatgctac
    19081 cagggtcgtc atgagtctta gggcattgga taaggttttg acaaacctga ttagtaatgc
    19141 aattaaatat tcagataaaa atgggcgcgt aatcatatcc gagcaagatg gctatctctc
    19201 tatcaaaaat acatgtgcgc ctctaagtga ccaagaacta gaacatttat ttgatatatt
    19261 ctatcattct caaatcgtga cagataagga tgaaagttcc ggtttgggtc tttacattgt
    19321 gaataatatt ttagaaagct atcaaatgga ctatagtttt ctcccttatg aacacggtat
    19381 ggaatttaag attagcttgt agacagatta gttttttatt aaagttcata tagggttaac
    19441 ataagtgtgt tattctttgt gtagataaaa gaaaggatac taatatggta ttagcgatta
    19501 ttttagtaac attctttatt cgattgattt ttttaaagcg ttcgatagag aatgagaaac
    19561 gaatccttag caatggcggg aaagaatttg gagttgagaa tacaaaacga ttaactatgg
    19621 ctcatattgt tttttatctc tcttgcttgg ttgaggcaat ggtgcacaag acaatttttg
    19681 atggcatggg catggttggt ttagtcttgc ttattttttc tatgctgatg ttaatgttgg
    19741 tgattcactt gttgggagat atttggacag tgaagcttat gcttgtcaat aatcacaaat
    19801 atgtagatca tatcttgttt aggacagtaa aacatcctaa ttacttttta aatattcttc
    19861 ctgagttgat tggcttgacc ttgttgagtc atgcttatgt gacttttgtt ttagtttttc
    19921 cagtttatgc agttattttg tatcgacgaa tagctgaaga ggaaaagcta ttacatgaag
    19981 ttataatccc aaatggaagc ataaagagat aaatacaaaa ttcgatttat atacagttca
    20041 tattgaagtg atatagtaag gttaaagaaa aaatatagaa ggaaataaac atgtttgcat
    20101 caaaaagcga aagaaaagta cattattcaa ttcgtaaatt tagtattgga gtagctagtg
    20161 tagtagttgc tagcttgttc ttaggaggag tagttcatgc agaaggggtt agaagtgggg
    20221 atacccccaa ggttacatct agtggggatg aagtcgagtc gcatctaaaa aaaatattga
    20281 gtgagatcca aacacaatta gatagaaaac gacatgccaa aactgtagcg ctaattaaca
    20341 agttgcaaga cattaagaga acgtattcgt ataaattaaa tgttttagaa gagaagccga
    20401 aagctgagtt gacgtcaaaa acaaaagaag agttaaccgc agcttttgag caatttaaaa
    20461 aagatacatt accaacagaa ccaggaaaaa aggtagcaga agctgagaag aaggttgcag
    20521 aagctgagaa aaaagccgag gatcaaaaag aagaagatcg tcgtaactac ccaaccaata
    20581 cttacaaaac gcttgaactt gaaattgctg agttcgatgt gaaagttaaa gaagcggagc
    20641 ttgaactatt aaaagagaaa gctaagaaac ctttaaacga gggcacaatt aagcaagcaa
    20701 aagagaaagt tgagagtaaa aaagctgagg ctacaaggtt agaaaacatc aagacagatc
    20761 gtaaaaaagc agaagaagaa gctaaacgaa aagcagcaga agaagataaa gttaaagaaa
    20821 aaccagctga acaaccacaa ccagcgccgg ctcctcaacc agaaaaacca gctccaaaac
    20881 cagaaaaacc agctccagct ccaaaaccag agaatccagc tccaaaacca gaaaaaccag
    20941 ctccagctcc aaaaccagag aatccagctg aacaaccaaa agcagaaaaa ccagctgatc
    21001 aacaagctga agaagactat gctcgtagat cagaagaaga atataatcgc ttgactcaac
    21061 agcaaccgcc aaaaactgaa aaaccagcac aaccatctac tccaaaaaca ggctggaaac
    21121 aagaaaacgg tatgtggtac ttctacaata ctgatggttc aatggcgaca ggatggctcc
    21181 aaaacaatgg ctcatggtac tacctcaaca gcaatggcgc tatggcgaca ggatggctcc
    21241 aaaacaatgg ttcatggtac tatctaaacg ctaatggttc aatggcaaca ggatggctcc
    21301 aaaacaatgg
//
LOCUS       DAVBCB010000039        21272 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVBCB010000039 DAVBCB010000000
VERSION     DAVBCB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 21272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.039"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..787)
                     /locus_tag="VQU28_001401"
     CDS             complement(<1..787)
                     /locus_tag="VQU28_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476747.1"
                     /translation="MLYVGIDIAKNKHDVTALNVPGKTVLKPLTFSNNKAGFELLDLS
                     LRQLNQDCLIALENTGHYAFNLLNFLHEQGYKVYTYTPLLIKEFAKSLSLRKTKTNKK
                     DAHGIALKLLSDPNREQFQHDNRQVELKILARHIHRLKKKQSDWKVQYTRCLDIIFPE
                     LDKIVEKHSEYTYQLLTCYPNPQKRLEAGFDKLIEIKRLTASKIQDILSVAPRSIGTT
                     SPAREFEIIKHYKRLIDKAETCVNDLMAEFNSVITTVTGIGNRL"
     gene            1042..1887
                     /locus_tag="VQU28_001402"
     CDS             1042..1887
                     /locus_tag="VQU28_001402"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HET2476748.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            complement(2034..3095)
                     /gene="dinB"
                     /locus_tag="VQU28_001403"
     CDS             complement(2034..3095)
                     /gene="dinB"
                     /locus_tag="VQU28_001403"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HET2476749.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIRDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKSVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGVSYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILLAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSKKERGVRLLGITMTGF"
     gene            3443..5767
                     /gene="pflB"
                     /locus_tag="VQU28_001404"
     CDS             3443..5767
                     /gene="pflB"
                     /locus_tag="VQU28_001404"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HET2476750.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            complement(6134..6352)
                     /locus_tag="VQU28_001405"
     CDS             complement(6134..6352)
                     /locus_tag="VQU28_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476751.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(6448..6686)
                     /locus_tag="VQU28_001406"
                     /pseudo
     CDS             complement(6448..6686)
                     /locus_tag="VQU28_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6679..6963)
                     /locus_tag="VQU28_001407"
     CDS             complement(6679..6963)
                     /locus_tag="VQU28_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476752.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            complement(7575..7745)
                     /locus_tag="VQU28_001408"
     CDS             complement(7575..7745)
                     /locus_tag="VQU28_001408"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476753.1"
                     /translation="MTKQVPKFTKDTAQLYTCKWLLYNKVTKMYDHTVVNHSVREYIT
                     DSISTNTIEETG"
     gene            complement(8395..9618)
                     /locus_tag="VQU28_001409"
     CDS             complement(8395..9618)
                     /locus_tag="VQU28_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HET2476754.1"
                     /translation="MITKQSAFLQNRATILEFLYRNPATSRTDIVNETGLTPATTTNI
                     IKELSEQSLIYETGDEFSEFSGSGRRRKTISITDNIPYVVGGIEINVLGIFLSLCDLQ
                     GKTLFETEILNEDYPISEINSTITNMIKTAIEYVPLETKLLGFGLSIPGHYNKDSGSI
                     ITNNPIWESFNLLNVIKRFNFPFIVKNNIDCMAIGQYLFNPHNTPDNFIFLHAGLGIY
                     TSFFTKEKIGASKNPYIGEIGHTIVELNGQYCECGKKGCLQTYISDAWLIKHAQLLFK
                     NSQLTVLKSLVKTEKDIHLDTLLTAYNLGDSALRQQIDKGVNLLATSIANLLLINPAD
                     KIYINSQLLNYQPFTHEVRDKIQDQLHFVPFTRNIEIEILPYNKHRGSIGACALAIVA
                     FFIEHSNVLQDIISP"
     gene            9786..11108
                     /locus_tag="VQU28_001410"
     CDS             9786..11108
                     /locus_tag="VQU28_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HET2476755.1"
                     /translation="MNNILAFLETKVAPFGEKVGNQRHLKAIREGFMMAMPLILVGSL
                     FLILISWPQEDFTNWLNSVGLLSILTTMNQSTVAIISLVACFGIAYRLSEGYGTDGPS
                     AGIIALSSFVLMAPRFSSMVYDKNGEQVKQLFGGAIPFSSLNASSLFMAITIGLVTAE
                     IYRMFIQRGITIKMPSGVPDVVSKSFSALLPGFTTFVLWALVLKGLEAAGVAGGLNGL
                     LGAIVGTPLKLIAGTLPGMILCVIVNSFFWFCGVNGGQVLNAFVDPVWLQFTTENQEA
                     VAAGQTLQHIITLPFKDLFVFIGGGGATIGLAICLFLFSKSRANKTLGKLAIIPSIFN
                     INTAILFTFPTVLNPIMLIPFIATPTINALITYVSMAVGLVPYTTGVILPWTMPPIIG
                     GFLATGASWRGALLQVVLILVSVAIYYPFFKIADKRNLEKEKATVGGK"
     gene            11110..12996
                     /locus_tag="VQU28_001411"
     CDS             11110..12996
                     /locus_tag="VQU28_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4838 domain-containing protein"
                     /protein_id="HET2476756.1"
                     /translation="MVIRVFDQQKNTYSSFALEELSYYINRVFKTDIELVEEKEADIF
                     VGLVNKEDRKDHVLISLDKGKGRIESNTIVGLLIGIYRMFHEFGVVYTRPGRGHDFVP
                     ELRFEDFLDKELSIDETASYYHRGVCIEGADSFENILDFIDWLPKIGMNSFFIQFENP
                     YSFLKRWYEHEFNPYLNKEKFSNELVQELSDRLDKELQKRGLIHHRVGHGWTGEVLGY
                     SSKFGWESGLSISEEKKPYVAEINGKRELFNTAPILTSLDFSNPDVADKMVEIIKDYA
                     KKRPDVNYLHVWLSDARNNICECENCRQELVSDQYIRILNQLDRALTSEGLDTKICFL
                     LYHELLWAPQKEKLDNPERFTMMFAPITRTFEMSYADVDFDNSIPTPKPYLRNKIILP
                     NSLEENLSYLFEWQKTFKGDSFVYDYPLGRAHYGDLGYMKISQTIYRDVSYLSNLHLN
                     GYISCQELRAGFPHNFPNYVMGEMLWKKTRSYEELIEEYFSALYGSNWQSVVEYLEKL
                     SSYSSCDYFNAIGSRQNDVLANHYYIAYNLADNFLPIIEENISKLLNSQKDEWKQLSY
                     HREYVVKMAKALYLQATGKTRQAQDEWRNVLNYIRGHELLFQSNLDVYRVIEVAKNYA
                     GFHL"
     gene            13164..13508
                     /locus_tag="VQU28_001412"
     CDS             13164..13508
                     /locus_tag="VQU28_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2476757.1"
                     /translation="MKSKEEINMLGFTIVAYAGDARSDLMDALAFARDGYFEQARELV
                     ESANDSIVSAHREQTNLLAEEASGDNFEVSFIMIHGQDTLMTTMLLYDQVKFFIDEYE
                     RIRKIEEHIGLQ"
     gene            13518..14930
                     /gene="lacG"
                     /locus_tag="VQU28_001413"
     CDS             13518..14930
                     /gene="lacG"
                     /locus_tag="VQU28_001413"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2476758.1"
                     /translation="MENLQVKALPKEFLLGAATAAYQVEGATRVDGKGTNMWDVYLQE
                     NSPFLPDPASDFYYRYEEDITLAAEHGLQALRLSISWVRIFPDIDGEPNILAVRYYHR
                     VFQSCLKHNVIPFVSLHHFDSPQKMLETGDWLNRENIDRFIRYARFCFQEFTEVKHWF
                     TINELMSLAAGQYIGGQFPPNHHFQLSEAIQANHNMLLAHALAVLEFHQLGIEGEIGC
                     IHALKPGYPIDGQKENILAAKRYDVYNNKFLLDGTFLGYYSEDTLFHLNQILEANNSS
                     FIIEDGDLEIMKRAAALNTMFGMNYYRSEFIREYKGENRQEFNSTGIKGQSSFKLNAL
                     GEFVKKPGIPTTDWDWNIYPQGLFDMLLRIKEEYPQHPVIYLTENGTALKEVKPEGEN
                     DIIDDSKRIRYIEQHLHKVLEARDRGVNIQGYFIWSLQDQFSWANGYNKRYGLFFVDY
                     ETQKRYIKKSALWVKGLKRN"
     gene            15000..16679
                     /locus_tag="VQU28_001414"
     CDS             15000..16679
                     /locus_tag="VQU28_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2476759.1"
                     /translation="MDKLVAAIEKQQGKFEKISTNNYMIAIKDGFIATMPLIMFSSFL
                     MIIIMIPKNFGVELPSPAIAWMRKVYMLTMGVLGIIVSGTVAKSLAGNVNRKMPPGKV
                     INDTSAMLAAICGYLVLTVTLVVDEKTGSTSLSTNYLGSQGLITSFVSAFITVNVYRF
                     CIKRDITIHLPKEVPGAISQAFRDIFPFSFVLLISGLLDIVSRFSLDVPFAQVFQQLL
                     TPIFKGAESYPAMMLIWFMCALLWFVGIHGPSIVLPAVTALQLSNMEENAQLLANGQF
                     PYHSLTPNFGNYIAAIGGTGATFVVPFILIFFMRSKQLKSVGKATITPVLFAVNEPLL
                     FGMPVILNPYLFVPFLMTPPVNVFLGKVFIDFFGMNGFYIQLPWAFPGPLGLLIGTNF
                     QLISFVFLSLMLVVDILIYLPFCRAYDRQLLVKEDIASSNDIILEEDTSEIMPGEIDV
                     IKSKELKVLVLCAGSGTSAQLANAINEGAQLAEVRVIANSGAYGAHYDIMGVYDLIIL
                     APQVRSYYREMKVDAERLDIQIVATRGMEYIHLTKSPSKALQFVLEHYQAV"
     gene            complement(16805..18244)
                     /locus_tag="VQU28_001415"
     CDS             complement(16805..18244)
                     /locus_tag="VQU28_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HET2476760.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDVSVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYLALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRIKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKTVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(18248..19597)
                     /gene="trkA"
                     /locus_tag="VQU28_001416"
     CDS             complement(18248..19597)
                     /gene="trkA"
                     /locus_tag="VQU28_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HET2476761.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            19785..20633
                     /locus_tag="VQU28_001417"
     CDS             19785..20633
                     /locus_tag="VQU28_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HET2476762.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     EQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            20648..21196
                     /locus_tag="VQU28_001418"
     CDS             20648..21196
                     /locus_tag="VQU28_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HET2476763.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLVGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
BASE COUNT         6427 a         3619 c         4366 g         6860 t
ORIGIN      
        1 ctaaacgatt cccaatccca gtgaccgtcg tgatgaccga gttgaactca gccatcaagt
       61 cattgacaca tgtttccgcc ttgtcaatga gcctcttgta atgtttgatg atttcgaatt
      121 cacgagcagg agatgttgtt ccgatagaac gaggtgcaac tgagaggata tcctgaattt
      181 tagaagcggt caatcgctta atttctatca gcttatcaaa tcctgcctca agccttttct
      241 gaggattagg gtagcatgtc aagagttggt aggtatattc tgaatgcttt tcaacgattt
      301 tatccaactc aggaaagatg atatcaagac aacgagtgta ttgtactttc caatcagact
      361 gttttttctt gagacgatga atatgtctag ccagtatttt tagttctact tgccgattat
      421 cgtgttgaaa ttgttcacga ttggggtcag aaagaagttt aagagcgatg ccatgagcgt
      481 ctttcttatt cgttttagtc ttgcgaagtg ataatgattt ggcaaattcc ttgatgagca
      541 aaggagtgta ggtgtaaact ttatatcctt gttcatgcag gaagttcagt agattaaagg
      601 cataatgtcc ggtattttca agagcgatga gacagtcttg gttgagctgt cgaagagaca
      661 gatctaagag ttcaaaacca gctttattat ttgaaaaagt gagtggttta agaacagttt
      721 ttcctggaac attcaaggct gtaacatcgt gtttattttt agcgatatca atgcccacat
      781 aaagcatggg agtacctcca gatatagtat ttcaagtcta cttggttatc cacgaatttt
      841 ttgccttgtt accttagacg agatcaaacg tctatgcgtt atcaaactca ttaccaattg
      901 aaacaaaagc tgtggttaga gcctttcgga aatcgtcaag cgattggagg aaatgaacta
      961 atccacagtg gcttattcca agtataccac ttgggctttg gcagtagcta actgcgctaa
     1021 ttataatata gggagtaatc tatgtatctt attgaaattt taaaatctat cttcttcggg
     1081 attgttgaag gaattacgga atggttgccg atttccagta caggtcactt gattttagca
     1141 gaggaattca tccaatacca aaatcaaaat gaagccttta tgtccatgtt taatgtcgtg
     1201 attcagcttg gtgctatttt agcagttatg gtgatttatt ttaacaagct caatcctttt
     1261 aaaccgacta aggacaaaca ggaagttcgt aagacttgga gactatggtt gaaggtcttg
     1321 attgctactt tacctttact tggtgtcttt aaatttgatg attggtttga tacccacttc
     1381 cataacatgg tttcagttgc tctcatgttg attatctacg gggttgcctt catctatttg
     1441 gaaaagcgca ataaagcgcg tgctatcgag ccaagtgtaa cagagttgga caagcttcct
     1501 tatacgaccg ctttctatat cggactcttc caagttcttg ctcttttacc agggactagc
     1561 cgttcaggtg caacgattgt cggtggtttg ttaaatggaa ccagtcgttc agttgtgaca
     1621 gaatttacct tctatcttgg gattcctgtt atgtttggag ctagtgcctt aaagattttc
     1681 aaatttgtga aagccggaga actcttgagc tttgggcaat tgtttttgct cttggtcgcg
     1741 atgggagtag cttttgcggt cagcatggtg gctattcgct tcttgaccag ctatgtgaaa
     1801 aaacacgact tcaccctttt tggtaaatac cgtatcgtgc ttggtagtgt tttgctactt
     1861 tacagttttg tccgtttatt tgtataagaa aaaccttgaa ggggcaactc ttcaaggttt
     1921 tatactcttc gaaaatctct tcaaaccgcg tcagctttat ctgcaacctc aaaacagtgt
     1981 tttgagcagc ctgcggctag cctcctagtt tgctctttga ttttcattga gctttaaaat
     2041 ccagtcatgg taatccccaa taggcggaca cctctttctt tcttgcttaa ttcttcatag
     2101 agttgcaggg ctatttggct tatctgacta gcatcttgtg ttttttgagc aagacttttt
     2161 cgtttggtaa gagttgaaaa gtcctcgtag cggattttca aaatgacaat ttttccagct
     2221 ttttcttgtt gatgtagatt gagagcgact ttttctgata gaagagtcag ctcttttttg
     2281 atatcttcct cagcaaggag aatcttcccg taggttttct ccttgccgat tgatttacgg
     2341 atgcgattgg atttgactgg agagttgtga atgccacgag cctttcgata cagatcatag
     2401 cctagtctac caaaacggtc tattagggtt acctcaggaa cttcaagtaa atcagcacca
     2461 gtaaaaacgc ccatttgatg aagacgttct actgtctttt ttcctactcc atgaaatttg
     2521 gaaatatcca tttgtttgag aaaatcctca gcctgttcag gtagaatcac tgtcaaacca
     2581 tgtggttttt gataatcact cgccatttta gctaagaatt tgttgtaaga aacgcctgcg
     2641 gaagcagtta gatggagttc ttgccagata tctttttgaa tgaggcgagc aattttgacc
     2701 gctgacttga taccgagttt attttctgtc acatccaaat aggcttcgtc aatgctcatg
     2761 ggttcaatca aatctgtata gcgcttaaaa atagctcgaa tctggagtcc cacagacttg
     2821 tatttctcat aattccctga gataaagaca gcctggggac aacgttcata agcttccttg
     2881 gaactcatgg cagaatggac accaaaagct cttgcctcat aactacaggt agaaacgact
     2941 ccccgtccac ctgtttgccg agggtcgctt ccaataatga caggttttcc tctgagttta
     3001 ggattatccc tgatttccac tgcagcaaaa aaggcatcca tgtcaatatg gatgattttt
     3061 cttgacaaat catttaacaa aggaaaaatc aacatgccta gcaccttttt atactcttcg
     3121 aaaatctctt caaaccacgt cagctctatc tgcaacctca aaacagtgtt ttgagcaatc
     3181 tgcggctagc ttcctagttt gcttttcgat ttccattgag tgttactgct tattttcttt
     3241 tattataccc ttttttctga aaaaaagaaa aaaggacttt attttttcaa aaatataata
     3301 cagtttgaaa taaaatatag actgttttag aaaagaaagt gtaaaaatag ggaattttca
     3361 cttgttgaaa tcggttacta tatggtatac ttgtcttatg aatgtaacag atgactgtta
     3421 ctagaaaaaa gaggacatta atatggttgt taagacagtt gttgaagcac aagatatttt
     3481 tgacaaagct tgggaaggct tcaaaggcgt agattggaaa gaaaaagcaa gtgtatcacg
     3541 atttgtacaa gctaactaca caccttatga tggagacgaa agcttccttg caggaccaac
     3601 agagcgttca cttcacatca agaaaattgt agaagaaact aaagcacact acgaagaaac
     3661 tcgtttccca atggacactc gtccaacatc tatcgctgat atccctgctg gatttatcga
     3721 caaagaaaat gaagttatct tcggtatcca aaacgatgaa ctcttcaaat tgaacttcat
     3781 gccaaaaggt ggtatccgta tggctgaaac tactttgaaa gaaaatggat acgaaccaga
     3841 cccagctgtt cacgaaatct tcactaaata tgtaacaaca gttaacgacg gtattttccg
     3901 tgcctacact tcaaatattc gtcgcgctcg tcacgcacac actgtaactg gtcttccaga
     3961 tgcatactca cgcggacgta tcatcggtgt ttacgcacgt cttgctcttt acggtgcaga
     4021 ctacttgatg caagaaaaag taaatgactg gaatgcaatc aaagaaatcg atgaagaaac
     4081 aatccgtctt cgtgaagaag taaaccttca ataccaagca ttgcaacaag ttgttcgcct
     4141 gggtgacctt tacggggttg atgttcgcaa accagcgatg aacgtgaaag aagcaatcca
     4201 atgggttaac attgctttca tggctgtctg ccgtgtgatt aacggtgctg ctacatctct
     4261 aggtcgtgta ccaatcgtat tggacatctt tgcagaacgt gaccttgctc gtggtacatt
     4321 tactgaatca gaaatccaag aattcgttga tgatttcgtt atgaaacttc gtacagttaa
     4381 atttgctcgt acaaaagctt atgaccaatt gtactcaggt gacccaacct ttatcacaac
     4441 ttctatggct ggtatgggta acgacggtcg tcaccgtgtt actaagatgg actaccgttt
     4501 cttgaacact cttgacaaca tcggtaactc accagaacca aacttgacag ttctttggac
     4561 tgacaaattg ccatacaact tccgtcgcta ctgtatgcac atgagccaca aacactcttc
     4621 tatccaatac gaaggtgtaa caacaatggc taaagacgga tatggtgaaa tgagctgtat
     4681 ctcatgctgt gtgtctccac ttgatccaga aaatgaagaa caacgccaca acatccagta
     4741 cttcggtgct cgtgtaaacg ttcttaaagc ccttcttact ggtttgaatg gtggttacga
     4801 cgatgttcac aaagactaca aagtatttga tatcgaacca atccgtgacg aagttcttga
     4861 atttgaatca gttaaagcga actttgaaaa atctcttgac tggttgactg acacttacgt
     4921 agatgccttg aacatcatcc actacatgac tgataggtac aactacgaag ctgttcaaat
     4981 ggccttcttg ccaactaaac aacgtgccaa catgggattc ggtatctgtg gatttgctaa
     5041 cactgttgat acattgtcag ctatcaaata cgctacagtt aaaccaatcc gtgacgaaga
     5101 tggctacatc tacgattacg aaacaatcgg tgactaccca cgctggggtg aagatgaccc
     5161 acgttcaaac gaattggcag aatggttgat cgaagcttac acaactcgtc tacgtagcca
     5221 caaactatac aaagacgcag aagctacagt atcacttttg acaatcacat ctaacgttgc
     5281 ttactctaaa caaactggta actcaccagt tcacaaaggt gtatacctca acgaagatgg
     5341 ttctgtgaac ttgtctaaac ttgaattctt ctcaccaggt gctaacccat ctaacaaagc
     5401 taaaggtggt tggttgcaaa acttgaactc actttctagc cttgacttta gttatgcagc
     5461 tgacggtatc tcattgacta cacaagtatc acctcgcgct cttggtaaga ctcgtgatga
     5521 acaagttgat aacttggtaa caatccttga tggttacttc gaaaacggtg gacaacacgt
     5581 taacttgaac gttatggact tgaacgatgt ttacgaaaaa atcatgtcag gcgaagacgt
     5641 tatcgtacgt atctctggat actgtgtaaa cactaaatac ctcactccag aacaaaaaac
     5701 tgaattgaca caacgtgtct tccacgaagt tctttcaatg gatgacgcct tggatgcatt
     5761 gagctaatca agttcttgaa taataaaaag gaaccctcgg tcaaacgact gagggttttg
     5821 tgcttgggat agtatgagca attccttcgg cgcaatatgc aatgtttttg ggaaggaata
     5881 gtggaccgaa tctgaaatat aatagattga aactagaata gtacaccttt acttctaaaa
     5941 cattcttaga aatcgatttg actgtcctga tcgatttgtc ctattcttat ttcattttac
     6001 tatagtccgc tgaaacggct caaacagtgc tagaaattga ctggaattct ctaagtgatt
     6061 tgttccgatt ttatttcatt tcatcataaa aataagatga ctggctaatt gacaatcatc
     6121 ttgtttgata ctgttaataa aattcgcgaa gtttatcttg attttcttta aaaaattttt
     6181 ctaaccaatc cataccataa gcataactaa aaccttctaa tagaacagag aggtcaatag
     6241 tatcaaatcc catgtctttt aaatatttaa aggtatcgaa tggtgaatga aagtttttgt
     6301 atttaataat tgcaccaata tcttgcaaat cctgctctct gatactcatc atcttcattc
     6361 ctaacacata ctcaataggg actactaaaa cggtcagatt ctcaaatgag tacaaggatt
     6421 cgcataaact taagggaggt tgtttgttca tatttgcttt gcaacatgat tgttgagcca
     6481 taactcttcg tggatgttaa gattaaactg ttttcctaca cgagcaataa tttcattgat
     6541 tttctgattt tggtcataaa aagcatcaac atcttgtgtg gcacgtaaac catgatattc
     6601 taagacataa ccataattgt taaagtcaga ttttcctgaa ttaatttctt atttagggct
     6661 tggaagaccg gtttcatttt atccatgttt gtatttctcc atcaattttt gaacattggt
     6721 taacttagca ggtttattta atgaaatcac atatttatga gttggagtat gctcttgaaa
     6781 ggcatacttc aagctaattt tttgaaaacg ggttagttct ttggagtcta gcaacttttc
     6841 tacatcttgg tttgttaaat aagaatagtc ttcaaaaata acttttcgca aatagcgaaa
     6901 ccaatggctt gcgttgaatt gtgattgttt ggatgcctgt atcatataat cgtaccaatc
     6961 cattgtcttc ctcctataaa atttagttta tattattgta gtttatattc aaaaatgtag
     7021 tttttcttaa ttagctcgta tctataggat gatactgaaa atcttcttgt tgctttaaat
     7081 cacaatcgaa aaattctgtt tttcatctta gaaaagaatg tttagaaatg taaaatgaac
     7141 cttttttctt atccgaaaac cttactaaag tatataattt aggcagtcaa ttctgtatga
     7201 taatgtttgt actatcgtcg agtttttgct tgattgccta acgagataaa ttttagaaga
     7261 gttttcggtg tgatttgaca agtttctgac tggaaggata gttacactag tattgtttat
     7321 taaaatgagc agatagagaa aatttggaat ctatttgctt attatcaatc gtttttttag
     7381 cttatttgtc ttagattggt atagaactga gctatggatt aatcttcttc tatcttatcc
     7441 tgttcctaaa tatgttggta gaaaaatgaa gcagttgtta cgagtgtctg tattgggaaa
     7501 aattttgtct atagaattct ttaaatgata tgaaggctag gaatgccaaa acactgacat
     7561 atttcgttta aagtctatcc agtctcttca attgtatttg tagaaatgct atcagtaata
     7621 tattcgcgaa cactatgatt tacaacagtg tggtcgtaca tctttgtaac tttattatac
     7681 aacaaccatt tacaggtgta aagttgagca gtgtctttag taaatttagg gacttgtttt
     7741 gtcatagttt tgatacctgt attcgttgtt tcatgagcat ttgacgtttc ttttcatgta
     7801 ggcatttcaa caattagaat gatatttttc cgaggatgaa cttatatatt gtgacttttt
     7861 agagttatga tgcctcttga tttgactacc gctatcaatt tcatccactt ttacagtttc
     7921 atggttttat gaaccgaaac atctaggtac aggttttagc atcaattgat tgtttttgag
     7981 ccatatgaag ttgaaacgtg gagatgcttt tcttgtgata acgccctatt caaagaggaa
     8041 taaccgtttt tggagagagt tcagtactat gtggcgctaa atactatatg taaaacatat
     8101 attaaacaag atttaactaa ggagaattgt aaaaacaaaa atgagaattt catataaagt
     8161 ttattctagg agtatgtatc ttgaataaaa atcgttattt aagttcaggg ttatgatgaa
     8221 tgtttttata ataataaaat gcagctagaa gaaaagttat tttaatataa atcactcaac
     8281 atagagagaa ggcggtttcc tatatggaag tcgtcttgtt ttcatattgt ctaaatacga
     8341 gatatctact acaggttata gttgatttaa acaggtctat agatttctaa tatattaagg
     8401 tgaaataata tcttgtaata cattgctatg ttctatgaaa aaagcgacga tagctaatgc
     8461 acaagctcct atacttccac gatgtttgtt gtaaggtaaa atttcaattt ctatattacg
     8521 agtaaaggga acgaagtgga gctggtcttg gattttatcc ctgacttcat gagtgaaagg
     8581 ttgataatta agcaattgac tgttgatata gattttatca gcaggattga tgaggaggag
     8641 atttgcaata gaagtggcta ataaattgac tcctttatca atttgttgac gtaaagcgga
     8701 gtcgcctaaa ttataagccg ttaaaagggt gtctaaatga atgtcttttt cagtctttac
     8761 aaggcttttt agtacagtta gttgggaatt tttaaataat aattgggcgt gtttgattaa
     8821 ccaagcatcc gaaatatatg tttgtaaaca accttttttt ccgcattcac aatattgccc
     8881 attcaattcg acaatggtgt gtccaatttc tccgatataa ggatttttag aggctcctat
     8941 tttttctttt gtgaaaaagg aagtgtaaat acctaatcca gcgtgtagga aaataaagtt
     9001 atcgggggta ttgtgtggat taaaaaggta ttgtcctata gccatacaat cgatattatt
     9061 ttttacaata aaaggaaaat tgaatctttt aattacattt aataaattaa aagattccca
     9121 tatggggttg tttgtaatga tacttccgga gtctttgtta taatgtccag gtattgataa
     9181 gccaaatcca agtaattttg tttccaaagg gacgtactct atagctgttt ttatcatatt
     9241 ggtaatggtg gaattgattt ctgaaatagg ataatcttca ttcaaaattt ctgtctcgaa
     9301 aagagttttc ccttgtaagt cacatagact gagaaagata cctaggacgt ttatttcaat
     9361 acctccaaca acatacggaa tattatctgt gatagaaata gtttttctac gtcttccaga
     9421 tccggaaaac tcgctaaatt catcaccagt ttcgtatatg agggactgtt cgcttagctc
     9481 ttttatgatg ttagtcgttg ttgctggagt aagtcctgtt tcattaacaa tatctgtacg
     9541 ggatgttgct ggatttcgat atagaaactc cagtatagtg gctctatttt gtaaaaaggc
     9601 tgattgtttt gtaatcattt gtttcctcct ttggtttatt ttatattaaa cataagaaaa
     9661 aatcaattaa aattattatt ttatacaaag aaaaaaatat tgtaaaaaca acaaaaaata
     9721 tattgacaac gctttctgtt agtggtatat ttaatttata aaaaataaaa aaaggagttc
     9781 cggtgatgaa caatatttta gcgtttttag aaacaaaagt cgctccgttt ggtgaaaaag
     9841 ttggcaacca acggcatttg aaagctattc gtgaaggatt tatgatggca atgcctttga
     9901 ttttagtcgg atctttattt cttattctaa tcagttggcc tcaagaggat tttacaaatt
     9961 ggctgaatag tgttggattg ctaagtatct tgacaactat gaatcagtca acagtagcga
    10021 ttatctcctt ggtcgcttgt ttcggtattg cctacaggtt gtcggaagga tatggtacag
    10081 atggtccgtc ggcagggatc atagccttat ccagttttgt attgatggca cctcgttttt
    10141 cgagtatggt ttatgataaa aatggggagc aggtcaagca gttatttggc ggcgcaatac
    10201 cattttctag cctgaatgca tcttctttgt ttatggcgat tactattgga ttggttacag
    10261 cagagattta tcgtatgttt atccagcgcg gaattacgat aaaaatgcca agtggtgtcc
    10321 cagatgtggt aagtaaatca ttttcagctc ttttacctgg ctttactact tttgttttgt
    10381 gggctctggt cttaaaaggt cttgaagcgg caggagttgc aggaggtctc aacggactcc
    10441 taggtgcaat tgttggaaca ccgcttaagt taattgcagg aacgcttcca ggtatgattc
    10501 tatgtgttat tgtaaactca ttcttttggt tctgtggagt taatggggga caagttttaa
    10561 atgcttttgt agatccagtt tggttacaat ttactacaga aaaccaagaa gctgtggctg
    10621 caggacaaac actccaacac attattacat taccgtttaa agatttattt gtatttattg
    10681 gtggcggtgg agcgactatt ggtcttgcga tttgtctctt cctatttagt aagagtcgtg
    10741 cgaataaaac attaggtaag ctagctatta taccgtctat ttttaatatc aatacagcta
    10801 ttctatttac gtttccaaca gttttaaatc cgattatgct gattccgttt attgctactc
    10861 ctacaatcaa tgccttaatt acctatgtat caatggctgt aggattagta ccctacacaa
    10921 caggtgtaat ccttccgtgg acaatgccac cgatcatagg aggcttcctt gcgacaggag
    10981 ctagttggcg aggagctcta ttacaagttg ttttgatttt ggtttctgta gcaatttatt
    11041 atccattctt caaaattgca gataaacgca atcttgaaaa agaaaaagct actgttggag
    11101 ggaaataaga tggttatcag agtatttgat cagcagaaaa atacttattc tagctttgcc
    11161 ttagaggaat taagttacta tataaatcgg gtctttaaga ctgacataga acttgtcgag
    11221 gagaaggaag cggatatttt tgtaggatta gtcaataaag aggatagaaa agaccatgtt
    11281 cttatctcat tagacaaggg taaggggaga attgagtcta atacaattgt aggtttgctt
    11341 attggaattt accgaatgtt tcatgaattt ggggttgtgt atactagacc agggcgcgga
    11401 catgactttg ttccagagtt acgatttgaa gattttttag ataaagagct atctatagat
    11461 gaaacagcca gttactatca taggggagta tgtatagagg gagcagattc atttgaaaat
    11521 atactagatt tcattgattg gctacctaag attgggatga acagtttttt cattcagttt
    11581 gaaaatccct attctttttt gaaacgttgg tatgaacatg agtttaatcc atacctaaat
    11641 aaagaaaaat tctcaaatga attagtacaa gaattgagtg ataggttgga taaagaattg
    11701 caaaaaagag gtcttattca tcatcgtgtt ggtcatggat ggacaggtga ggttttaggt
    11761 tactcttcaa aatttggctg ggaatcaggt cttagtattt cagaggagaa gaaaccctat
    11821 gtcgctgaaa taaacgggaa acgagaatta tttaatacgg ctccgatttt aaccagtctg
    11881 gatttttcaa atccagatgt ggcggataag atggtagaaa ttatcaagga ttatgccaag
    11941 aaaagacctg atgttaacta cttacatgta tggttgtcgg atgctcgtaa taatatttgt
    12001 gaatgcgaaa actgtagaca agaattggtt tcggatcagt atattcgtat tctcaatcaa
    12061 ttggataggg ctttaacgag tgagggatta gatacaaaga tttgttttct gctttaccat
    12121 gagttgttat gggcgcctca gaaagaaaaa ttagataatc ctgaacgctt taccatgatg
    12181 tttgcaccga ttacaagaac atttgaaatg agttatgcag atgtagattt tgacaattcc
    12241 atacctacgc ctaaacctta tctgcgtaat aaaattatac ttccgaattc tcttgaggaa
    12301 aatttatctt atctttttga gtggcaaaaa acatttaaag gagatagttt cgtatatgac
    12361 tatcctttag ggcgtgctca ttatggcgat ttaggctata tgaaaattag tcaaaccatt
    12421 tacagagatg tatcttatct ttctaaccta catttgaacg gttacatttc gtgtcaagaa
    12481 ttacgtgccg gattccctca taattttcct aattatgtta tgggggaaat gctctggaag
    12541 aagacaagaa gttatgaaga attgattgaa gaatactttt ctgctttgta tgggtcaaat
    12601 tggcagtctg ttgttgaata tttagaaaaa ttatccagtt attcctcatg tgattatttt
    12661 aatgcaattg gcagtcgtca aaatgatgtt ttagcgaatc attattatat agcttacaat
    12721 ctagctgata attttttgcc aattattgag gaaaatattt ctaagttatt aaatagtcaa
    12781 aaggatgaat ggaaacagct cagttatcat cgtgaatatg ttgttaagat ggcgaaggct
    12841 ttatatcttc aagcaaccgg aaaaacaagg caagctcaag atgaatggag aaatgtgttg
    12901 aattatatcc gtgggcacga attgctattt caatctaatt tggatgttta tcgtgtaatt
    12961 gaagtagcaa aaaattacgc tggtttccac ttataaatca taagtataga aaatgaacca
    13021 aggtattcag agaagattga tcctaaatat tatgaaattt aaggattttt aagatattta
    13081 gggtcgactt tctatttata tcgtagcgaa gtcattttaa taatgatgtg taaaagatgg
    13141 atcaagattg aggaggaaga aagatgaaat caaaagaaga aataaatatg cttggtttta
    13201 caattgtcgc ttacgcagga gatgcaaggt cagatttgat ggatgctttg gcgtttgcga
    13261 gagatggata ttttgaacag gcaagagaat tggttgagtc tgcaaacgac tcaatagtgt
    13321 ctgcccatcg agaacagact aatttattag cggaggaggc atctggagat aattttgaag
    13381 tgagttttat catgattcat ggtcaagata ccttgatgac aacgatgcta ttgtatgatc
    13441 aggtcaagtt ttttattgat gaatatgaac gaattcgaaa gattgaagaa catattggtt
    13501 tgcaatgagg attagtcatg gaaaatttac aggttaaagc cttaccgaag gagtttttat
    13561 taggagctgc taccgctgct tatcaagtag agggtgcaac tagggtagat ggcaaaggaa
    13621 caaatatgtg ggatgtttat ttgcaagaaa acagtccgtt cttaccagat ccagctagtg
    13681 atttttatta tcgttacgaa gaggatataa ctttggcggc agaacatggt ttgcaggctt
    13741 tgcgtttatc tatttcttgg gttcgtatat ttcctgatat agatggggag cctaatatat
    13801 tagctgttcg ttattaccat agagtctttc agtcttgctt aaaacataat gtgattccgt
    13861 ttgtttcttt acatcatttt gattcgcctc agaaaatgtt agaaacaggg gattggttga
    13921 atagagagaa tattgatcgt ttcatacgat atgctcgctt ttgtttccaa gaatttacag
    13981 aagtcaagca ttggtttaca atcaatgaac tgatgtctct tgctgcaggt caatatatag
    14041 gaggtcagtt tcctccaaat catcattttc aattatctga agcaattcaa gcgaatcata
    14101 atatgttgtt ggcgcatgct cttgcagtcc tcgaatttca tcaattagga attgagggag
    14161 agataggttg tattcatgct ttaaagccag gctatcctat tgatgggcaa aaagaaaata
    14221 ttttggcagc taaacggtat gatgtttata ataataaatt tctattagat ggaacttttt
    14281 tgggctacta cagtgaggac acgctttttc acttgaatca aatattggaa gctaataatt
    14341 ctagctttat tattgaagat ggtgatttag aaattatgaa gagagctgca gctcttaata
    14401 cgatgtttgg gatgaattat tatcgttcag aatttattcg tgaatacaaa ggtgaaaata
    14461 gacaagaatt taattcaaca ggaataaaag gacagtcttc ttttaaatta aatgctctag
    14521 gtgaatttgt aaaaaaacct ggtattccga caacagattg ggattggaat atttatcctc
    14581 aagggttatt tgatatgttg cttcgtatca aagaagaata tcctcaacat ccggtcattt
    14641 atttaactga aaatggtaca gcccttaaag aagttaagcc agagggcgag aatgatatta
    14701 ttgatgacag taagagaatc cgttatattg agcaacattt acacaaagtt ttagaggctc
    14761 gagatagagg agtcaatatt caaggctatt ttatatggtc tttgcaagat caattttctt
    14821 gggcgaatgg ctacaataag cgatatggtc ttttctttgt tgattatgaa acacagaaga
    14881 gatatattaa gaaaagtgct ctttgggtaa aagggctaaa acggaattaa ggttagcgat
    14941 ttgactgatg tttaatatat tttaactatg agcttaaatt ttttacagga ggagttttta
    15001 tggataagtt agtcgctgcc attgaaaagc aacaagggaa atttgaaaaa atttctacta
    15061 ataattatat gatagctatt aaagatggat ttattgctac tatgccttta attatgtttt
    15121 caagcttttt gatgattatt attatgattc ctaaaaattt cggagtagag ttaccgagtc
    15181 cagctattgc ctggatgagg aaagtgtata tgctaaccat gggagttttg ggtattattg
    15241 tttcagggac agttgcaaag tcattggctg gaaatgttaa tagaaaaatg cctcctggaa
    15301 aggtaataaa tgatacttct gcaatgttgg cggccatatg tggctatctt gtattaactg
    15361 taacgcttgt agttgatgag aaaacgggat ctacaagttt gtcgacaaac tatttaggat
    15421 ctcaaggatt gataacttcg tttgtcagtg cctttattac tgtaaatgtt taccgattct
    15481 gtattaagcg agacattact attcatttac ctaaggaagt tcctggggct atatcacaag
    15541 cttttagaga tatttttcct ttttcttttg ttttacttat tagtggtttg ttagatattg
    15601 tatctcggtt tagtttagat gttccttttg cccaagtatt tcaacaacta ttgactccta
    15661 tttttaaggg ggcagaatca tatcctgcta tgatgttgat ttggtttatg tgtgctttgc
    15721 tttggtttgt tggaattcat ggaccatcta ttgtcttacc tgctgttaca gctttacaac
    15781 tgagcaatat ggaagagaat gctcaacttc ttgcaaatgg gcagttccct tatcattctt
    15841 taacacctaa tttcgggaat tatatcgctg ctattggagg aacgggggct acctttgttg
    15901 taccatttat tttgattttc tttatgcggt ctaaacaatt aaaatcggta ggtaaagcta
    15961 caattactcc tgttttattt gcggtaaatg aacctcttct atttggtatg cctgttattt
    16021 tgaatcccta tctttttgtc ccttttttga tgactccacc agtgaatgta tttctaggaa
    16081 aggtatttat tgatttcttt ggaatgaatg gattttatat ccagttacct tgggcctttc
    16141 ctggtccctt gggattgtta attggaacga attttcaact tatctccttt gtatttttat
    16201 ctttgatgtt agttgtcgac atattgattt atttgccatt ctgtagagcg tatgatagac
    16261 agttactggt gaaagaagat attgcaagct caaatgatat tattttagag gaggatacaa
    16321 gtgaaataat gcctggtgag atagatgtaa taaaaagtaa ggagttgaaa gtactggttc
    16381 tttgtgcagg gtctggaaca agtgcgcaat tagccaatgc aattaacgag ggcgctcaat
    16441 tagcagaagt tagagtgatt gcgaattcag gagcgtacgg agctcattat gatattatgg
    16501 gtgtttatga tttaattatt ctggccccac aagttcggag ttattataga gagatgaagg
    16561 tggatgcaga aagattagat attcagatag ttgctaccag aggaatggaa tatattcatt
    16621 taacaaagag tccaagtaaa gccttacaat ttgtattgga gcattaccaa gctgtgtagt
    16681 aagtttttcc atcttttatt tgagtaaaga ttttgcttac agataggctt ggatttaaaa
    16741 acgttccccc ttttttaata taagaatccc tctttcacaa ttgtaaaaag agggattttg
    16801 tattttatct cttagaccaa gttctcttca taaagagaag gaggattggg taaatctcca
    16861 agcgccctgc aatcattgca aaggatagga gaatttttga gatgggacta aagattgaga
    16921 aactagaagt ggttcctaga ataggcccga tattattgaa acagctaaag acagcgctgg
    16981 tcacgaccag aaaatcattg ctatctaggc tgacaataaa gataagcgct agcaaaatca
    17041 tagcatagat gacaaagtac ttgagaatct tatgctgggt atctttgtca atcaccgttt
    17101 tattaacatg gagggtcaaa acacggtggg gcgataggat tgacaaaatt tggtttttgg
    17161 caatttttga aaggatgagg cctcgaataa tcttgagtcc acctgcagtt gatccagcag
    17221 agccaccgat tgccatgagg aaaaggagga taaactggga gaagaggggc cagttggtaa
    17281 tatctccata tccaaaacca gttgttgtaa tgatgttgga aacctggaag aaggtcattt
    17341 caaagctctt tgaaaaccct gggtagaggt agagggtgtt gaggctaatc aagcctgtag
    17401 aaaccagtac aatgaccaag taagccctaa gctcttcatc tccaaagaag gccttgatgc
    17461 gacggagcat gaggtagtag tagaggttga aatttactcc aaaaaccaga actccgatac
    17521 tgaccagata ggtaatcagt gagctgccat agtgggcaat tccgtcgtta tagacggtaa
    17581 agcctccagt tcccgctgtc cccatagcaa tgacaaaact atcgtagaga ggcataccag
    17641 ctagataata gatgatgaca aagagggaga agagagctag ataaaggaga tagagaatct
    17701 gggcagtgtt ttttagtttg gatacaacct tgccaaaaac aggacctgga acctcagcct
    17761 tcatcacctc taggtggcta tttttggcat tgtccataat agcaagtgca aaaacaagca
    17821 ctcccatccc tccaatcaag tgggtaaaac ttcgccagaa gaggagggaa cgactgagaa
    17881 ctgaaacgtc gttcaaaata cttgctccag tagtcgtaaa gccggaacta atctcaaaaa
    17941 aggcatcaat aaggctgggg atttgcccag aaaagacaaa ggggagacca ccaaagaaag
    18001 accaaaggat ccaacagagg gcaacgatca agactccctc cttggcataa atccgttgat
    18061 tttttggctt ctgtaaactc cctgaaccgc ctaacaatac gagaatccct atggtcgaaa
    18121 agagggctgt aaagacttgg ctcgattcac ggtaatagac agcgatagcc acaggaacca
    18181 agaggagaac agcttcaatc aaaagtaact ttgaaaggag gtaacgaatc atacttttat
    18241 tcatttctta cctcgcaatc aagtcataaa tcttggtgat gtttggcaac aaggttgtta
    18301 ctaggagctt gtctccaact tccaacatat cctccccagt tgggaaaata gtcttgccct
    18361 ttcgaataat ggctgcaata agaacccctt ttttcaattt cagttgagaa agaggtttgg
    18421 cagtcatttt attggcttcc ttgatatgga attgcagggt ttcgatttgg ccattggcta
    18481 gatggtgcat ggcttgaagg tctgaatact gggcattaac tcgaccacga ataaagtgca
    18541 taatcgtatc tacagcgatg cttttaggtg tgatgatact tgaaaaatca ggcgcattga
    18601 taatctcgag gagactggta cgattgacct tagtaatatt tttctgtaca cctaccctgt
    18661 caaggaacat agatgtaatc agattttcct catcgactcc tgttagagtc gcaacggcat
    18721 catagtgttg agcactttct tccagcagga tatcttttgc ggttccatct ccttgaacga
    18781 tgtagagatt tgggaatttc tcgctaaaga agctggcgat ttcaggattg atttcaatga
    18841 cttttgtatc gatacgacta tctttgagaa taccaagtag ataataggca attctacctg
    18901 ccccaacgat gagaaggctc ttcacggcgc gtgatttaaa ataattatgg aagagtatca
    18961 tatcgacacg gttaccagtg acaaagattc tatctttatc ctgtacagtc atgtcaccgc
    19021 ttggaatgat aatttgatga tccctctcta tcgcacagac aatgacatta ccaaattttt
    19081 tacgaaaatc agaaatgggc atttggcaaa gaccgctggt ggacttgacg acaaattcca
    19141 tgaggctaac gcgtccacca gcaaagcgtt cgacagacag ggcgttgggg aagtcaatga
    19201 tattcgcgat agcacgggca gccaagagct caggattaac gataagagaa aaaccgagaa
    19261 tattcttttc cttgaaataa gagttagaat attcagggtt ccgcacccga acgatagttt
    19321 ctttagctcc cattttcttg gctagaactg ctgcaatcat gttgacttca tcgtgctcag
    19381 tcagggcgat aaagatatca caatcttgga cgctggcttg ctcaagaatg gcaaaatcgg
    19441 ccccgttacc aaggatacca atgatatcaa agcgactgac aatatgattg agaacagctt
    19501 cgtcttgctc aatcagcaaa acatcatgct tttctgcaac caaggagcga cagagggcaa
    19561 aaccaacttt tccccctccg acaaggataa ttttcataat aaaacctact ttttcatgat
    19621 gtaactatca tacccttttt caagaaaaaa tgcacctact agctaataac aagagctttt
    19681 agtgaaaatt cgctataaga taaaactata ccctaaccaa ttgaaatagc tattagcgac
    19741 tttctctgaa atatggtatg ataaaggata tacaaggaga taaaatgaat aataatttac
    19801 tggtattaca atcagacttt ggtctggttg atggtgcggt atcggctatg attggagtgg
    19861 ctttagaaga gtctccaacc ttaaaaatcc atcacttgac gcacgatatc acgccttata
    19921 atatttttga ggggagctat cgtctctttc agacggtgga ttactggcct gagggaacga
    19981 cgtttgtatc ggttgtcgat ccaggtgtcg gttcgaaacg taagagtgta gttgccaaga
    20041 ctgcaaaaaa tcaatacatt gtcacgccag ataatgggac gctttccttt atcaagaaac
    20101 acgttggcat tgtagccatt cgtgagattt ctgaggtggc caataggcgt caaaacacag
    20161 agcattctta taccttccac ggtcgtgatg tctatgccta tactggtgct aaactggcca
    20221 gtggtcacat tacttttgag gaagtagggc cagagctcag tgtggaacag attgtagagc
    20281 ttccagtcgt agcgaccatc atagaagatc atctggtgaa gggagccatt gatattctgg
    20341 atgtgcgttt cggttcgctt tggacctcta tcacacggga agaattttac aagttggaac
    20401 cagaatttgg tgatcgtttt gaagtgacca tctatcatgc tgatatgctg gtctatcaaa
    20461 atcaggttgt ctatggcaaa tcatttgcag atgtgagaat tgggcaaccc atcctttaca
    20521 tcaactctct ctatcgttta ggtttggcta tcaaccaagg ttcctttgcc aaggcctata
    20581 atgtaggtgt cggttcatct tggaccattg aaataaagaa aatagaagga taaaatagga
    20641 gaatagtatg gaaatcaaat ttacaattaa acaagttgtt gctgtcggaa ttggcgctgc
    20701 cctctttgtc gtcatcggga tgatcaacat tccaacccct gttccaaata caagcatcca
    20761 gcttcagtat gcggtacaag cgctactttc tattattttt ggaccgatta tcggtttgct
    20821 tgttgggttg attggtcatg caatcaagga ctctcttgtt ggttatggtc tatggtggac
    20881 ttggattatc gctagcggac tctttggttt agttgtggga ctttttagaa agtacgttcg
    20941 cgtgatcaat ggtgtttttg actggaaaga tattcttatt tttaacctca ttcaactact
    21001 tgcaaatgcc cttgtttggg gtgtcttggc accacttgga gatgttgtga tttatcaaga
    21061 agcggcagaa aaagtatttg cacaaggaat tgttgcggga attgccaatg gtgtatctgt
    21121 agctattgca ggaactcttc tcttacttgc ctatgcagga acccaaactc gtgcaggaag
    21181 tttgaaaaag gactaatact caatgaaaat caaagagcaa actaggaagt tagccgcagg
    21241 ttgctcaaaa cagtgttttg aaattgtgga ta
//
LOCUS       DAVBCB010000040        20855 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVBCB010000040 DAVBCB010000000
VERSION     DAVBCB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20855
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.040"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQU28_001419"
     CDS             <1..128
                     /locus_tag="VQU28_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2476764.1"
                     /translation="FRSLENLEQAIVDYIDYYNNKRIKVKLKELSPVQYRTKSFG"
     gene            complement(176..2719)
                     /locus_tag="VQU28_001420"
     CDS             complement(176..2719)
                     /locus_tag="VQU28_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HET2476765.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLFPFILTILFFTLKSIKFHVKLIYVIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVAGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(2781..4403)
                     /locus_tag="VQU28_001421"
     CDS             complement(2781..4403)
                     /locus_tag="VQU28_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2476766.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            4609..5634
                     /gene="trpS"
                     /locus_tag="VQU28_001422"
     CDS             4609..5634
                     /gene="trpS"
                     /locus_tag="VQU28_001422"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HET2476767.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            5787..7265
                     /gene="guaB"
                     /locus_tag="VQU28_001423"
     CDS             5787..7265
                     /gene="guaB"
                     /locus_tag="VQU28_001423"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HET2476768.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(7316..8413)
                     /gene="recF"
                     /locus_tag="VQU28_001424"
     CDS             complement(7316..8413)
                     /gene="recF"
                     /locus_tag="VQU28_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HET2476769.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(8416..8784)
                     /gene="yaaA"
                     /locus_tag="VQU28_001425"
     CDS             complement(8416..8784)
                     /gene="yaaA"
                     /locus_tag="VQU28_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HET2476770.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKIDIPDMNIDILLTQSTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            8943..10193
                     /locus_tag="VQU28_001426"
     CDS             8943..10193
                     /locus_tag="VQU28_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2476771.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSYLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHCPSKYGDEKHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSQLDLFSGFLRMYAGIDRENRNQARKMMNNQLLDL
                     KKGYFTELELEQTKEMIRRSLLLSQDNQSSLIERAYQNALLGKSSADFKSWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            10190..11473
                     /locus_tag="VQU28_001427"
     CDS             10190..11473
                     /locus_tag="VQU28_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HET2476772.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTLVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLDLLDELITSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKLTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            11515..12336
                     /locus_tag="VQU28_001428"
     CDS             11515..12336
                     /locus_tag="VQU28_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2476773.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            12347..12892
                     /gene="pgsA"
                     /locus_tag="VQU28_001429"
     CDS             12347..12892
                     /gene="pgsA"
                     /locus_tag="VQU28_001429"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HET2476774.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            12889..13716
                     /locus_tag="VQU28_001430"
     CDS             12889..13716
                     /locus_tag="VQU28_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2476775.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            13701..14540
                     /locus_tag="VQU28_001431"
     CDS             13701..14540
                     /locus_tag="VQU28_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HET2476776.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPNQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            14533..15327
                     /locus_tag="VQU28_001432"
     CDS             14533..15327
                     /locus_tag="VQU28_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2476777.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            15386..16204
                     /gene="mreC"
                     /locus_tag="VQU28_001433"
     CDS             15386..16204
                     /gene="mreC"
                     /locus_tag="VQU28_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HET2476778.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKVSQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            16204..16698
                     /gene="mreD"
                     /locus_tag="VQU28_001434"
     CDS             16204..16698
                     /gene="mreD"
                     /locus_tag="VQU28_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HET2476779.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            16792..17970
                     /locus_tag="VQU28_001435"
     CDS             16792..17970
                     /locus_tag="VQU28_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HET2476780.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            18194..18973
                     /gene="rpsB"
                     /locus_tag="VQU28_001436"
     CDS             18194..18973
                     /gene="rpsB"
                     /locus_tag="VQU28_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HET2476781.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            19052..20092
                     /locus_tag="VQU28_001437"
     CDS             19052..20092
                     /locus_tag="VQU28_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HET2476782.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            20339..20680
                     /gene="tnpB"
                     /locus_tag="VQU28_001438"
                     /pseudo
     CDS             20339..20680
                     /gene="tnpB"
                     /locus_tag="VQU28_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT         6642 a         3341 c         4293 g         6579 t
ORIGIN      
        1 gctttagatc tttagaaaac cttgaacaag ctattgtgga ctacattgat tactacaaca
       61 acaaacgaat taaggtaaaa ctaaaagaac ttagccctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actttttggg gtcagtacat taactggtcg attttttatg
      181 ccttagatga ctttcgtctg tgattataga ttccaaatag taagagagaa gtaaaggaac
      241 agattgctcc agtaataaaa ccattgggaa tgaaggaaag tgtaatagtt cctttcccct
      301 tgggaatgtc aactttcata aatccagttt gagcttgttt aatttctatt ttcttaccat
      361 cttggtaggc agaccaacct ttgtcataag gaatggtgaa gaaaatagat gtatcttgtt
      421 ggacatcata tgtagcaaaa accttgtttt tagaagttga tactgtgaca ggttgttctt
      481 taattttttg aattgcctcg gtgaaagttt tggtatctaa acgatagaag gtaggagatt
      541 caaatgatac ttgtgaattt ccagggaaac taacattgat attgaaagtt tttttctctt
      601 tagtatatcc tagattaaag aaggagaaga cattatcagt tgtaaaagtc tttttttcac
      661 catttacaag gatgtcaacc ttcttttgtt tatcgttaga aaagtgaagg tttatgaaag
      721 agagataaac ttggctgttt tctggaactt caatttgata ctggattgct gcatcttcat
      781 ttgaagaact tgtgacacta atcaaatcat tagtattttc tgttttttca taaggtattg
      841 gagaaaaata atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca
      901 aggtatgttc attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa
      961 gagagtattg attttcatat agggtaagat tatctttttg atagatatct ttaaagccat
     1021 acttatcaat aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa
     1081 tactattatt tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat
     1141 ctaaagtaga gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt
     1201 tgaatttcat actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat
     1261 ttccaatata tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag
     1321 aagcaaatcc ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt
     1381 caaccagtat aaataaagag attagaatgg caaatagatt cacagatata aactttttga
     1441 taactgcaag gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca
     1501 aatctgttaa aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgctacta
     1561 caagaaaaag cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt
     1621 ctgctgctgt gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt
     1681 ttggagtatg catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta
     1741 gaaatgcaaa gaatattaca tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa
     1801 aaaataaaat ggtcaaaata aagggaaata gtccaacaaa aatcattggg atggccccat
     1861 actttgttgt gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac
     1921 tttcagtttg aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaata
     1981 gagtgggaag agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat
     2041 caagaacaga tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa
     2101 taagaaacaa tactgtcata tatccaaaat aataattttg aataaataag attgacagac
     2161 ttgtaaagta caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta
     2221 aaggaatcaa gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac
     2281 tcatcagagc ataggaagta gataaggcta gttttaaagt ctgagggata gattgaaaca
     2341 atttattcaa actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca
     2401 gatagacagc atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac
     2461 tacccaagta ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt
     2521 aaaacagact actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt
     2581 gatgaaagcc atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata
     2641 gatatactcc agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg
     2701 tttttaaaaa tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca
     2761 actgagtttt gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct
     2821 gcattttcta ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat
     2881 aagacaatga tatggttagc caaagtttga ataaactcgt ggtcatggct ggcaaagatg
     2941 attgattctt taaagttttt cagtccatca ttcaagcttg agatagattc caagtccaag
     3001 tgatttgttg gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc
     3061 atgacacgaa ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca
     3121 gagaagagca tacggccgag gaaaccacgt aggaaagtat tgtcatcttc ttctttactt
     3181 gcgaattgac gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct
     3241 tttggtaggt aagattgact agttgtaact ccccacttga cagttccttc atagtcaata
     3301 tctcccatga ttgcacgaat taatgcagtc gtttgaatat cattttgtcc aataagtgct
     3361 gtcttatcat ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc
     3421 tttacagtta gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag
     3481 ttgataaatg gatatttacg actagatggt acaatctctt ctagctcaat cttatcaagc
     3541 attttcttac gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg
     3601 aattcttgca attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta
     3661 gcagcaagct cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt
     3721 ccaaagtcaa ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat
     3781 actacgataa ctgtgttatc aaagtcaatc aagaagtctt ctaaccaagt aatcgattgg
     3841 atatccaaac cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct
     3901 ttggcgagga gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt
     3961 aattcttctg gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa
     4021 cctccaagtt cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag
     4081 aaatcttcct tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt
     4141 cccatgataa cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga
     4201 acagagagac gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca
     4261 gctaaaattt ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta
     4321 tttccttctg taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt
     4381 gaaacatcag atactgtaag caatgttttt ctcctatatg tgtaatatat ttattctact
     4441 agaaaataca gaaatattca aatttttatt tgtcaatttt gtgtaaatta tatttacagt
     4501 atcctttaca caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt
     4561 cattaaaaaa atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc
     4621 attattttaa caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt
     4681 ctcaaaaatc gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct
     4741 gaccaacaag ccttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga
     4801 aatgtggctt tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt
     4861 caaagccaga ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta
     4921 gcacgtttgg agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa
     4981 agcattccga caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc
     5041 aaggctaatt atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa
     5101 attgttcgtt cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt
     5161 tatccagaaa atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct
     5221 aaatcactaa ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta
     5281 atgagtatgt atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga
     5341 aatatggttt tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct
     5401 gatatgaaag aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta
     5461 cttgaaatat tagaacgtga actgggtcct attcgtgagc gccgtattga atttgctaag
     5521 gatatgggag aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg
     5581 ggtcaaaccc tatctgaggt aaaaggggca atgggacttc attactttaa ctaagtttat
     5641 tttacaagaa actatcattt ctatctcgaa gaaaagatag tttttttatt tacccctgta
     5701 acatttgaca aatttttata gaatggtatg atagatacaa tataaaaaga gtcaagctca
     5761 aaaagaaaga aaagaggaaa cttcgaatgt ctaattggga cactaaattt ttgaaaaaag
     5821 gttttacctt tgatgatgta ttgcttattc cagctgaaag tcatgtgttg cctaacgatg
     5881 cagatttaac aactaaattg gcagataatt tgactttaaa tatcccaatt attaccgctg
     5941 ccatggacac agttacagag agtcaaatgg ccattgctat tgctcgtgca ggcggtctcg
     6001 gagttatcca taaaaacatg tcaattgctc aacaagcaga cgaggttcgt aaggtaaaac
     6061 gttctgaaaa tggagttatt attgatccgt tcttcttgac gcctgaacat acaattgctg
     6121 aagcagatga gcttatgggt cgttaccgca tcagtggtgt tccagttgtt gaaacacttg
     6181 aaaatcgtaa attggttggt attttgacaa accgagatct tcgttttatt tcagattata
     6241 atcaaccaat ttcaaaccat atgactagtg aaaatcttgt tactgctcct gtgggtacgg
     6301 atcttgcaac ggctgagagt attcttcaag agcatcgtat tgaaaaactt ccgttggtcg
     6361 atgaagaagg cagtctttct ggtttgatca ctatcaaaga tattgaaaaa gttattgagt
     6421 ttccaaatgc ggctaaagat gagtttggtc gtcttctagt tgcaggtgca gtaggtgtta
     6481 cttcagatac atttgaacgt gcagaggctc tttttgaggc aggagcggat gcgattgtta
     6541 ttgatactgc acatggtcat tctgcaggtg tcttgcgtaa aattgccgag attcgtgctc
     6601 atttcccaga tcggactttg attgctggaa atattgctac tgctgaaggt gcacgtgccc
     6661 tttatgaagc gggtgtagac gttgttaagg ttggtattgg accaggttct atctgtacta
     6721 ctcgtgtgat tgctggtgtt ggtgttccgc aagtaacagc tatctacgat gctgcagctg
     6781 ttgcgcgcga atatggtaaa acgattattg ctgacggtgg gatcaagtat tctggagata
     6841 ttgtaaaagc acttgctgca ggtggaaatg ctgttatgct tggatctatg tttgctggaa
     6901 ctgatgaagc tccaggcgaa actgaaatct tccaaggacg taaattcaag acttaccgtg
     6961 gtatgggatc aattgctgct atgaagaaag gttcaagcga ccgttatttc caaggttctg
     7021 tcaatgaagc aaacaagctt gttccagaag gaattgaagg tcgtgttgct tataaaggag
     7081 cggcagctga tattgttttc caaatgattg gtggtattcg ctctggtatg ggttactgtg
     7141 gtgcagctaa ccttaaagaa ctacacgata atgctcaatt tattgaaatg tctggtgctg
     7201 gtttgaaaga aagccatcct catgatgtgc aaattactaa tgaggcacca aattattcta
     7261 tgtaaaaaac aatgaaaaga actccagtga aaacaggagt tcttttacaa tgttgtcaat
     7321 ttccatttac agcagcttta ccatcctgaa tagtgaagat acttagattt tctggcagat
     7381 tttgaagatg gtctaagctt gttgttgtga taaaggtttg gattgattga gaaatcgttt
     7441 ctaataattt taactgtcta gtgttgtcaa gttcactcat cacatcgtca agcagtaata
     7501 taggagattc tgtggtaatg ctttccatta attcgatttc tgctaatttt atcgagagga
     7561 cgagactacg atgttgacct tggcttccga aactagcatc catcccattt atataaaaag
     7621 aaatgtcatc tcgatgagga ccgacaccag tattcttttt aaataaatct ctggatctac
     7681 ttttttctaa agcaattttg aaagattcgg ataagttttg tttgtcagtt atattgacag
     7741 aagattgata ggatattgac aactcttcga tctgattaga gagttcaaaa tgtttcttac
     7801 gcccaaatga ttctagtttt tttatgaaat ctaagcggtg attcattaca cgacatccat
     7861 aatcaactag ctgatcatct aacacagaaa ggaatgtttc atctattttt tgagctgatt
     7921 ttaggtaagt gtttctttgc tttaggatgt ggttataatt ggttaagtca gataaataga
     7981 ttggcttaat ttgcccaagt tccatatcaa tgaattttcg tcgaatcgaa ggtgctcctt
     8041 taattagttg taaatcttca ggagcaaata agacaacatt catgtgtcct acataatctg
     8101 aaaggcgtgc ctgttttaag tgattaactt ttgttacacg gcctttttgt gttagttcga
     8161 tttctagggg aatggatcca gtttttttct gaacgagacc tgaaagatga agttgttcct
     8221 catcaaaatg aatgagattt ttatctgttc gagttcgatg actacgcgtt aaggctaaaa
     8281 aatagatagc ctctaacatg tttgtttttc cttgtgcatt acgtcccaaa aagacattca
     8341 atttaggatt aaagtctatt ttcgtttctt tgtaattacg aaaagtcttg agagatagat
     8401 gttgtagcca catgattatc taccagggaa tcgtggagct tgttggcttt taggtgatga
     8461 agcaggtttc gatttgtctt ttttaactcc cttattcatc tttttgacaa gtttagcgat
     8521 ccgttctttt tcaactttat cagcttggta ttcatcttgt tcttcagaag tagattgtgt
     8581 caacaagatg tcaatgttca tgtcagggat gtcaatttta tcgccaatac gaagtttttt
     8641 accacgacga ctctctaatt ccctattaaa gtaaacagaa tgttcagaga gaaatgattt
     8701 aatagctcct ccgctatgtg taattccaag ttctttgagt agtgcttgga gggtaataaa
     8761 ttcttcaaat aatttgtatt ccataattca cctcaatctt tggtattata ccatattttc
     8821 ctaaaaatag tgagagatac agcgagaaat gtaagcgatt tttatttcaa aagtgttaca
     8881 gggaacaaga aaatttcagg ttttcgtgat ataatagaag tctgtatata aggagataaa
     8941 tcatggagtt agtgcatgga atttcaacac attttatcca atcaaaaaag tttaaaacaa
     9001 acaaaattac cgtgcgtttt accgctccat tatcccttga tacgattgca ggtcacatgt
     9061 tgagtgcaag tatgctagag actgctaatc agatgtaccc cacttctcaa gatttgagga
     9121 gacacttggc cagtctatac ggtacagata tgtcaaccaa ttgtttcaga agagggcaaa
     9181 gccacattat agaattgaca tttacctatg ttcgtgatga gtttttaagt aggaaaaacg
     9241 tgctaacttc tcagattttg gaacttgtaa aagaaactct tttttcaccc gtagtagttg
     9301 ataatgggtt tgatccggcc ttatttgaaa ttgagaaaaa acaattgcta gcaagtttag
     9361 cagctgatat ggatgattct ttttattttg cacataaaga attggataaa ttgttttttc
     9421 atgatgaacg tcttcaattg gaatatagtt atttacgaaa tcgtatttta gctgaaactc
     9481 cacaaagttc ttattcttgt ttccaagaat ttttagccaa tgatcgaata gatttctttt
     9541 tcctaggtga ttttaatgag gttgaaattc aaaatgtatt agaatcattt ggctttaaag
     9601 gtcgaaaagg agatgtgaag gttcagtatt gtcaacctta ttctaatatc cttcaggaag
     9661 gtatggttcg gaaaaatgtg ggacaatcca ttttggaatt aggttatcat tgcccttcta
     9721 aatatggtga tgagaaacat ttacccatga ttgtaatgaa tggtttactt ggtggatttg
     9781 ctcactctaa actttttaca aatgtccgtg aaaatgctgg attagcttat actatttcaa
     9841 gtcagctcga tttatttagt ggattcttga ggatgtatgc tggtattgac cgagaaaatc
     9901 gtaaccaggc tcgtaaaatg atgaataatc aactgcttga tttaaaaaaa ggttatttta
     9961 cagagcttga gttagagcag accaaggaaa tgattcgtcg gtctttgtta ctttctcaag
    10021 ataaccaatc ttcattgatt gaacgtgctt atcaaaatgc cttacttgga aaatcttcag
    10081 cagattttaa aagttggatt gcaaaactcg agcaagttga caaagatgct atttgtagag
    10141 cagctaacaa tgtgaaacta caagcgattt actttatgga aggaatagaa tgacaaaggt
    10201 tgtttttgaa gaaaaatact atccagctgt aaaagaaatg gtttatcgaa ctcgtttggc
    10261 aaatggattg acagtagctc ttttgcctaa aaaggaattt aaagaggttt acgggagtgt
    10321 aactgtacag tttggttcgg tagatacgct tgtcacagaa gttgatggag atgtaaaaca
    10381 atatcctgca ggaattgctc attttcttga acataaatta tttgaaagag aagattctag
    10441 cgatttgatg tcggctttta cgagtctagg tgcagatagt aatgccttta caagctttac
    10501 aaaaacaaac tatctttttt cagcaacgga ttatttttta gaaaatttag atttacttga
    10561 tgaattgata acatcagcac actttactga agcttccatt ctgacagagc aggatattat
    10621 tcagcaagaa cgagaaatgt accaagatga tccagattcg tgtttattct tttcaacttt
    10681 agcgaatttg tatcctggta cacctttagc aactgatata gttggaagtg aggagtccat
    10741 ttcccaaatc aatctaacta atttgcaaga aaattttaca aagttttaca aacctgtaaa
    10801 catgtctctg tttttagttg gtaattttga tgtggagcga gtacaggact attttgaaag
    10861 caaagaactg aaagattcag attttcagga agtagcaaga gaaaagttgt ttttacagcc
    10921 tgtaaagcta acagatagta tgagaatgga agtatcttct cccaaactag cgattggagt
    10981 tagaggtaag cgagaagttt ctgaagcgga ttgctatcga catcatattt tattaaaatt
    11041 attgtttgca atgatgtttg gttggacttc ggatcgtttt caaaaatgtt atgaatcagg
    11101 taaaattgat gcgtccttat ctctggaagt tgaaataaca agtcgctttc attttgtcat
    11161 gttgacaatg gatacgaaag agccagttgc tttgtctcat caatttagga aggctattcg
    11221 taattttaca aaggatttag atattacaga ggaacattta gatattatca aaagagagat
    11281 gtttggcgaa tttttcagta gcatgaactc tcttgaattt attgcaacgc aatatgatgc
    11341 ttttgaaaat ggtgagacaa tttttgattt gccgaaaatt ttacaggaaa ttactttaga
    11401 ggatgtcctt gatgctggac atcatttaat agatgatggt gacatagttg attttacaat
    11461 atttccatcg tagtaaccta tcataataga cactagaaag aagggatgac aagtatgaga
    11521 aaaaaaacaa ttggagaggt tttacgatta gctagaatca atcagggatt gagtttagat
    11581 gaattgcaga aaaagacaga aatccagtta gatatgttgg aagcaatgga agcagacgat
    11641 ttcgatcaac ttccaagtcc tttttacacg cgttctttct tgaaaaaata tgcatgggct
    11701 gttgagttag atgaccaaat tgttttggat gcttatgatt ctgggagtat gattacttat
    11761 gaggaagtag atgttgatga agatgagttg acaggtcgta gacgttcaag taagaaaaag
    11821 aagaaaaaaa catcattttt acctttattt tattttatcc tttttgcttt atcgatttta
    11881 atttttgtga cttattatgt ttggaactat attcaaactc aaccagagga gccttctctt
    11941 tctaattaca gtgtggttca atcaacaagt tcaactagct ctgttcccca ctcctcaagt
    12001 agtagttctt ctagtataga atcagctata agtgtatcag gcgaaggaaa tcatgtagaa
    12061 atcgcttata agacaagtaa ggaaacagtt aaattgcaat tggcagtttc agatgttaca
    12121 agttgggtca gtgtttcaga aagcgaactt gagggcggtg taaccttatc gccaaagaag
    12181 aaaagtgcag aagcaacagt tgcaactaaa agtcctgtaa caattacgtt aggtgttgta
    12241 aaaggtgttg atttgacagt agataatcag actgttgatt tatcgaaatt aacagctcag
    12301 actggacaaa tcactgtaac ctttactaaa aattaaggaa aaacgaatga aaaaagaaca
    12361 aattcccaat ctcttaacaa taggtcgaat tctctttata cctattttta tctttatttt
    12421 aacgatagga aattcgatag agagtcatat agttgcagct attatctttg ctgttgccag
    12481 tattaccgac tatttagatg gatatttagc tcgtaaatgg aatgtggtca gtaattttgg
    12541 taaatttgca gatcctatgg cggataagtt actagttatg tcggctttta ttatgttgat
    12601 tgagttaggt atggctccgg cttggattgt tgcagtgatt atatgtcgtg agttagctgt
    12661 gacaggttta aggcttttat tggttgaaac tggtggaaca attttagcag cagcaatgcc
    12721 tggaaaaatt aaaactttta gtcagatgtt tgctattatt ttcttgctat tacattggac
    12781 tttgcttggt caagttctac tttatgtagc cttatttttc actatctact ctggctatga
    12841 ctattttaag ggtagtgcct atgtatttaa agggacattt ggttcgaaat gaaatcaata
    12901 attgatgtaa aaaatctttc ttttcgctat aaagaaaatc agaactacta cgatgtgaag
    12961 gatattacgt ttcacgtgaa acgtggagaa tggctttcga ttgtagggca taatggtagt
    13021 ggtaaatcaa cgacggttcg attaattgat ggcttactgg aagcagaatc cggagagatt
    13081 gtaattgatg gccaacgttt gactgaggaa aatgtttgga atatacgtcg tcaaatcggt
    13141 atggtttttc aaaatccaga caatcaattt gttggagcga ctgttgaaga tgatgttgct
    13201 tttggtttgg aaaatcaggg actttctcgt caagaaatga aaaagagagt ggaagaagct
    13261 ctggctttag ttggcatgtt ggactttaaa aagagagagc cagcgcgtct atcaggtggt
    13321 caaaagcaac gtgtggccat tgcaggtgtt gtagccctaa gaccagctat tttaatctta
    13381 gatgaagcaa cgagtatgtt ggatcctgag gggcgtagag aacttattgg gacagtaaaa
    13441 ggaattcgaa aagactatga tatgacggtc atttctatta cccatgattt ggaagaagtc
    13501 gccatgagtg atcgcgtatt ggtcatgaaa aaaggggaaa ttgaatcaac tagtagtcca
    13561 agggagcttt tctctcgaaa tgatttagat caaattggat tagacgatcc ttttgctaat
    13621 caattaaaaa aatctttgag ccagaatggc tatgatttac ctgaaaatta tttgacagaa
    13681 agtgagctag aggataagct atgggaattg ctctagaaaa tgtgaatttt acatatcaag
    13741 aaggtactcc cttagcttca gcagctttgt cggatgtttc tttgacgatt gaagatggct
    13801 cttatacagc tttaattggg cacacaggta gtggtaaatc aactatttta caactcttaa
    13861 atggtttatt ggtgccaaat caagggagtg tgagggtttt tgatacctta atcacctcga
    13921 cttctaaaaa taaagatatt cgtcaaatta gaaaacaggt tggcttggta tttcagtttg
    13981 ctgaaaatca gatttttgaa gaaacggttt tgaaggacgt tgcttttgga ccgcaaaatt
    14041 ttggagtttc tgaagaagat gctgtgaaga ctgcgcgtga gaaactggct ctggttggaa
    14101 ttgatgaatc actttttgat cgtagtccgt ttgagctgtc agggggacaa atgagacgtg
    14161 ttgccattgc aggcatactt gccatggagc cagctatatt agtcttagat gagccaacag
    14221 ctggtctaga tcctctaggg agaaaagagt tgatgaccct gttcaaaaaa ctccaccagt
    14281 cagggatgac catcgtcttg gtaacgcatt tgatggatga tgttgctgaa tatgcgaatc
    14341 aagtctatgt aatggaaaag ggacgtttag taaagggggg caaaccaagt gatgtctttc
    14401 aagacgttgt ttttatggaa gaagttcagt tgggagtacc taaaattacg gccttttgta
    14461 aacgattggc tgatagaggc gtgtcattta aacgattacc gattaagata gaggagttca
    14521 aggagtcgct aaatggatag tatgatttta gggcgttata tcccagggga ttcgattgtt
    14581 caccgattgg atccacgtag caaattgctt gctatgatgc tactgatttt gattgtgttt
    14641 tgggctaata accccttgac gaatctgatt ctttttatag cgacagggat atttattgcc
    14701 ttgtcaggag tatctctttc attttttatt cagggcttga agtctatgtt tttcttgatt
    14761 gccttcacaa ctatttttca actatttttc atttctaatg ggaatgtttt atttgagttt
    14821 tcgtttgtga gaatcacgga ttatgctttg caacaagctg ggattatttt ttgtcgcttt
    14881 gtattgatta ttttcttttc aactttgtta accttaacaa ccatgccttt aagtttggca
    14941 tcagctgtcg aagctttatt agcaccttta aagcgtgtga aagttccagt tcatgaaatt
    15001 ggattgatgc tgtctatgag tttgcgtttt gtcccaacct tgatggatga tacgactcgg
    15061 attatgaatg cacagaaagc tcgtggagtg gattttggag aaggaagcat cgttcaaaaa
    15121 gtaaaggcga tgattcccat tttgattcct ctttttgcga caagtttaaa acgtgcagat
    15181 tccttggcta tcgctatgga agcgcgtggc tatcagggtg gaaaaggcag aagtcaatac
    15241 cgacaattga aatggactct aaaggatacg ctgaccattc ttgttattct cgtacttggt
    15301 tgttgtttat ttttcttaaa atcttagtag ctttcaggaa ttgataaaaa gttactgtaa
    15361 cagtttttga tataaaggta ggaatatgaa ccgttttaaa aaatcaaaat atgtcattat
    15421 tgtttttgtc actgttctgc ttgtgtcagc tctcttagcg acgacttatt caagtacaat
    15481 tgtgacaaaa ttaggagatg gaatctcatt ggttgataga gttgtacaaa aaccttttca
    15541 gtggtttgat tctgtcaaat cagatttggc tcatttgaca cgaacatata atgaaaatga
    15601 aagtttgaag aaacagcttt accaattaga agttaaatca aatgaggtgg aaagtttaaa
    15661 gacagaaaat gaacaactgc gccaattgct tgatatgaag tctaaattgc aagccacaaa
    15721 gactttagca gcagatgtta ttatgcgttc tccggtatct tggaagcagg agttgacctt
    15781 agatgcaggt agatcaaaag gtgcttctga gaacatgtta gctattgcaa atggtggctt
    15841 gattgggagt gtttcaaaag tagaggagaa ctctactata gtcaaccttc tgacaaatac
    15901 ggaaaatgct gataagattt ctgttaaaat tcaacatggc tctactacaa tttatggaat
    15961 tattattggc tatgacaagg aaaatgacgt tcttaaagtt agccaattaa atagtaatag
    16021 cgatattagt gcgggagata aggtgactac tggtggatta ggaaacttta acgttgctga
    16081 tattcctgtt ggtgaagtgg ttgccacaac gcatagtaca gactatttga cacgagaagt
    16141 aactgttaaa ttgagtgcag atactcataa tgtagatgtg atagaattag tggggaattc
    16201 ataatgagac agttgaagcg agttggagta tttttattgc ttcctttctt tgttctaatt
    16261 gacgcccata ttagccagct tctgggctca tttttccccc atgtacattt ggctagtcat
    16321 tttctttttc tatttctctt atttgagacg atagaagtat cagagtatct ctacctagtc
    16381 tattgttttg ttataggctt ggtttatgat gtttactttt tccatctaat agggattaca
    16441 actctcttat ttatcttatt gggagccttc cttcataaat tgaatagtgt tattttgttg
    16501 aatcgttgga caagaatgct agctatgatt gtactgacat tcctgtttga aatgggtagt
    16561 tatcttttgg cttttatggt agggttgaca gtagatagca tgtcgatttt tatagtctat
    16621 agcttggtac cgacgatgat tttaaatttt ttatggatta ctgtttttca atttattttt
    16681 gaaaaatatt atctataaga acgacatata aatgtaacaa aggcgtaata tttattaggc
    16741 ctttttttgg tatactagta ttgtctttaa aagaaggagt atctacgtaa tatgaagaaa
    16801 aaaatcttag cgtcactttt attaagtaca gtaatggttt ctcaagtagc tgttttaaca
    16861 actgcgcatg cagaaacgac tgatgacaaa attgctgctc aagataataa aattagtaac
    16921 ttaacagcac aacaacaaga agcccaaaaa caagttgacc aaattcagga gcaagtatca
    16981 gctattcaag ctgagcagtc taacttgcaa gctgaaaatg atagattaca agcagaatct
    17041 aagaaactcg agggtgagat tacagaactt tctaaaaaca ttgtttctcg taaccaatcg
    17101 ttggaaaaac aagctcgtag tgctcaaaca aatggagccg taactagcta tatcaatacc
    17161 attgtaaact caaaatcaat tacagaagct atttcacgtg ttgctgcaat gagtgaaatc
    17221 gtatctgcaa acaacaaaat gttagaacaa caaaaggcag ataaaaaagc tatttctgaa
    17281 aaacaagtag caaataatga tgctatcaat actgtaattg ctaatcaaca aaaattggct
    17341 gatgatgctc aagcattgac tacgaaacag gcagaactaa aagctgctga attaagtctt
    17401 gctgctgaga aagcgacagc tgaaggggaa aaagcaagtc tattagagca aaaagcagca
    17461 gctgaggcag aggctcgtgc agctgcggtg gcagaagcag cttataaaga aaaacgagct
    17521 agccaacaac aatcagtact tgcttcagca aacactaact taacagctca agtgcaagca
    17581 gtatctgaat ctgcagcagc acctgtccgt gcaaaagttc gtccaacata cagtacaaac
    17641 gcttcaagtt atccaattgg agaatgtaca tggggagtaa aaacattggc accttgggct
    17701 ggagactact ggggtaatgg agcacagtgg gctacaagtg cagcagcagc aggtttccgt
    17761 acaggttcaa cacctcaagt tggagcaatt gcatgttgga atgatggtgg atatggtcac
    17821 gtagcggttg ttacagctgt tgaatcaaca acacgtatcc aagtatcaga atcaaattat
    17881 gcaggtaatc gtacaattgg aaatcaccgt ggatggttca atccaacaac aacttctgaa
    17941 ggttttgtta catatattta tgcagattaa tttacagagg gactcgaata gagccctctt
    18001 ttcaggtttt accgtgacaa tccctattaa aaattatatc aaaatagctt gaaaatattg
    18061 gaaaagtatg gtaaaatgaa aattgtcgtg tgaacgataa tactcattct tgatgaattg
    18121 tgaagcagtt gcccttgggt cgttttgcga gttgaagtca agaagaggaa aaaaacaaaa
    18181 aggagaaata ctcatggcag taatttcaat gaaacaactt cttgaggctg gtgtacactt
    18241 tggtcaccaa actcgtcgct ggaatcctaa gatggctaag tacatcttta ctgaacgtaa
    18301 cggaatccat gttatcgact tgcaacaaac tgtaaaatac gctgaccaag catacgactt
    18361 catgcgtgat gcagcagcta acgatgcagt tgtattgttc gttggtacta agaaacaagc
    18421 agctgatgca gttgctgaag aagcagtacg ttcaggtcaa tacttcatca accaccgttg
    18481 gttgggtgga actcttacaa actggggaac aatccaaaaa cgtatcgctc gtttgaaaga
    18541 aattaaacgt atggaagaag atggaacttt cgaagttctt cctaagaaag aagttgcact
    18601 tcttaacaaa caacgtgcgc gtcttgaaaa attcttgggc ggtatcgaag atatgcctcg
    18661 tatcccagat gtgatgtacg tagttgaccc acataaagag caaatcgctg ttaaagaagc
    18721 taaaaaattg ggaatcccag ttgtagcgat ggttgacacc aatactgatc cagatgatat
    18781 cgatgtaatc atcccagcta acgatgacgc tatccgtgct gttaaattga tcacagctaa
    18841 attggctgac gctattatcg aaggacgtca aggtgaggat gcagtagcag ttgaagcaga
    18901 atttgcagct tcagaaactc aagcagattc aattgaagaa atcgttgaag ttgtagaagg
    18961 cgacaacgct taatttatac aaatagtaat tacctaggag ggcggggctt agcccggctc
    19021 tcctattttc aaaaaatata ggagaattaa aatggcagaa attacagcta aacttgtaaa
    19081 agagttgcgt gaaaaatctg gtgccggtgt tatggacgct aaaaaagcgc ttgtagaaac
    19141 agacggtgac atcgaaaaag cgattgaatt gcttcgtgaa aaaggtatgg ctaaggcagc
    19201 taagaaagct gaccgtgttg ctgcagaagg tttgactggt gtttatgtta acggtaatgt
    19261 tgcagcagtt attgaagtaa acgctgaaac tgacttcgtt gcaaaaaacg ctcaattcgt
    19321 tgaattggta aatactacag ctaaagtcat tgctgaagga aaacctgcta acaacgaaga
    19381 agctcttgct ttgataatgc cttcaggtga aactcttgaa gctgcatacg tatctgcaac
    19441 agcaactatc ggagagaaaa tctcattccg tcgctttgca ttgattgaaa aaacagacgc
    19501 acaacacttt ggagcatacc aacataacgg tggacgtatc ggtgttattt cagttgttga
    19561 aggtggagac gaagcacttg ctaaacaatt gtcaatgcac atcgcagcga tgaaaccaac
    19621 agttctttct tacaaagaat tggatgagca attcgttaaa gatgagttgg cacaattgaa
    19681 tcacgttatc gaccaagaca acgaaagccg tgcaatggtt aataaaccag ctcttccaca
    19741 cttgaagtat ggatcaaaag ctcaattaac tgatgatgtt attgctcaag ctgaagctga
    19801 catcaaagct gaattggctg cagaaggcaa accagaaaaa atctgggaca aaattattcc
    19861 aggtaaaatg gatcgcttca tgcttgataa cactaaagtt gaccaagctt acacacttct
    19921 tgcacaagtt tacatcatgg atgacagcaa gacagttgaa gcataccttg aatcagttaa
    19981 cgcttcggta gttgagtttg ctcgctttga agttggtgaa ggtatcgaga aagctgcaaa
    20041 cgacttcgaa gctgaagttg cagctacaat ggcagcagcc ttgaataact aataatagaa
    20101 aggaagcaaa ttgcttcctt ttttgtatag ggcttagatt tttcctgaaa ttgttttttc
    20161 cccagtctca ctcgattaga ataattaaaa caaaaagccc tataataagg gctaagtgca
    20221 tttaggagac tatacttcaa attcatagta agcgcgttca taacaaggta tctatcctaa
    20281 ttccccaact tccagtatac tagataaaaa ataattatcg gagtaaaggt attgtactat
    20341 gataattcaa ctaagtgatt taggtcaagt tcaccttgtt tgtggcaaga cagatatgag
    20401 gtagggaata gactcattag cctatgtagt taaaacccac tttgaattgg attctttctc
    20461 cggtcaagtc tttctctttt gtggtgaacg taaagaccgc tttaaagccc tttactggga
    20521 tggtcaagga ttttggctac tatataaacg ctttgagaac ggaaaactga cttggctaag
    20581 tacagaaaag gatgtcaaag ctctcgcacc tgaacaagta gattggctta tgaagggctt
    20641 ttctatcact ccaaaatata gtatattgaa actagaatag tacacctctg cttctaaaac
    20701 attgttagaa atcgatttga ctgtcctgat cgatttatcc tgttcttatt tcattttact
    20761 ataaatctat cagaaagttg tgatttctat tgaaatgagg actttctttt tacactcatc
    20821 tgctttcaaa aagcattcta gtccgtctcc gatta
//
LOCUS       DAVBCB010000041        20838 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVBCB010000041 DAVBCB010000000
VERSION     DAVBCB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.041"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            182..658
                     /locus_tag="VQU28_001439"
     CDS             182..658
                     /locus_tag="VQU28_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2476783.1"
                     /translation="MKLSLYSEVEHYHFLSSYSLNDITYTDTPKNIIEKSKKDKYRYP
                     IVILNKDHKVIGFFCLHLNTGPKEYGYYQNDYALIRGFSIHDNFRNQGYGSNALNEIF
                     EFIDSTLKLEINHIILAVNEKNILAQKAYRNSGFSVVKKDLEGKRGKLIIMEKSRN"
     gene            complement(1332..1724)
                     /locus_tag="VQU28_001440"
     CDS             complement(1332..1724)
                     /locus_tag="VQU28_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2476784.1"
                     /translation="MWKKKKVKAGVLLYAVTIAAIFSLLLQFYLTRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKTDKKEEVATDSSEKAEKKIRREA"
     gene            complement(1702..2163)
                     /locus_tag="VQU28_001441"
     CDS             complement(1702..2163)
                     /locus_tag="VQU28_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HET2476785.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVGQLEAELDRSQFEKVEGNRLYMKQDDKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            complement(2126..2386)
                     /locus_tag="VQU28_001442"
     CDS             complement(2126..2386)
                     /locus_tag="VQU28_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476786.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVSINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            complement(2391..2819)
                     /locus_tag="VQU28_001443"
     CDS             complement(2391..2819)
                     /locus_tag="VQU28_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HET2476787.1"
                     /translation="MEGQIVKSMIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVE
                     EQIFFMEFEELYRETQKRSVASQQKTSLNLDGQMISNGSQKLTVPKGIQAPSGQSITF
                     DRAGGNSSLAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            complement(2788..3114)
                     /locus_tag="VQU28_001444"
     CDS             complement(2788..3114)
                     /locus_tag="VQU28_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HET2476788.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLDKNEDATLSKLQGDGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            complement(3116..4132)
                     /locus_tag="VQU28_001445"
     CDS             complement(3116..4132)
                     /locus_tag="VQU28_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HET2476789.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            complement(4080..5021)
                     /locus_tag="VQU28_001446"
     CDS             complement(4080..5021)
                     /locus_tag="VQU28_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HET2476790.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            complement(5097..5462)
                     /locus_tag="VQU28_001447"
     CDS             complement(5097..5462)
                     /locus_tag="VQU28_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HET2476791.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            complement(5613..6671)
                     /locus_tag="VQU28_001448"
     CDS             complement(5613..6671)
                     /locus_tag="VQU28_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HET2476792.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQU28_001449"
     CDS             complement(6834..7985)
                     /gene="nagA"
                     /locus_tag="VQU28_001449"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HET2476793.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            complement(8138..9955)
                     /locus_tag="VQU28_001450"
     CDS             complement(8138..9955)
                     /locus_tag="VQU28_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HET2476794.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMIFLLSATAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQU28_001451"
     CDS             complement(10056..11198)
                     /gene="tgt"
                     /locus_tag="VQU28_001451"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HET2476795.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMLLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAECGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFRKYFVEKYGYNKSGRNF"
     gene            11328..12185
                     /locus_tag="VQU28_001452"
     CDS             11328..12185
                     /locus_tag="VQU28_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HET2476796.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSGFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQU28_001453"
     CDS             complement(12213..12857)
                     /gene="pcp"
                     /locus_tag="VQU28_001453"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HET2476797.1"
                     /translation="MKVLVTGFEPFGGEKGNPTLEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSVTPERVAINQDDARISDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRPTTPTMSLVDIRRGIEAAIGAMIEHGDQELKLVGGETH"
     gene            complement(12964..13332)
                     /locus_tag="VQU28_001454"
     CDS             complement(12964..13332)
                     /locus_tag="VQU28_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HET2476798.1"
                     /translation="MSIITIILTTIVALEHFYIFYLESIATRSDATSRVFNMEKEELA
                     HPSVSSLFKNQGIYKALLGVFLWYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKII
                     LKQGGSAILALISILLFKYT"
     gene            complement(13343..13768)
                     /locus_tag="VQU28_001455"
     CDS             complement(13343..13768)
                     /locus_tag="VQU28_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2476799.1"
                     /translation="MIEIQDLLYQLRLSEQASTQLFEKRLGISLTRYQILLFLLEHSP
                     CNQMAVQERLKIDQAALTRHFKILETEGLVERHRNPENQREVLVEAAKYAKEQLVVNP
                     PLQHIKVKEEMESILTEFERTELSRLLNKLVLGIENIEI"
     gene            complement(14193..15335)
                     /locus_tag="VQU28_001456"
     CDS             complement(14193..15335)
                     /locus_tag="VQU28_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2476800.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQGTTPAEKQETQASPQAASAVEATTTS
                     SEAKEVASSNGATAAVSTYQPEETKIISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPERSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            complement(15501..16121)
                     /locus_tag="VQU28_001457"
     CDS             complement(15501..16121)
                     /locus_tag="VQU28_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2476801.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNTFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            complement(16125..17405)
                     /locus_tag="VQU28_001458"
     CDS             complement(16125..17405)
                     /locus_tag="VQU28_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2476802.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKLTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            complement(17466..18950)
                     /locus_tag="VQU28_001459"
     CDS             complement(17466..18950)
                     /locus_tag="VQU28_001459"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HET2476803.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALREKLTT
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGEKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNNAEKTAELMNALNTQGQYKLTDFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQAVTGDVTKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMQAAVEAYLGL"
     gene            complement(19026..20303)
                     /locus_tag="VQU28_001460"
     CDS             complement(19026..20303)
                     /locus_tag="VQU28_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HET2476804.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            complement(20410..20493)
                     /locus_tag="VQU28_001461"
     tRNA            complement(20410..20493)
                     /locus_tag="VQU28_001461"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20457..20459),aa:Leu,seq:caa)
     gene            complement(20503..20574)
                     /locus_tag="VQU28_001462"
     tRNA            complement(20503..20574)
                     /locus_tag="VQU28_001462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20540..20542),aa:Gln,seq:ttg)
     gene            complement(20588..20660)
                     /locus_tag="VQU28_001463"
     tRNA            complement(20588..20660)
                     /locus_tag="VQU28_001463"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20625..20627),aa:His,seq:gtg)
     gene            complement(20675..20745)
                     /locus_tag="VQU28_001464"
     tRNA            complement(20675..20745)
                     /locus_tag="VQU28_001464"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20711..20713),aa:Trp,seq:cca)
     gene            complement(20750..20830)
                     /locus_tag="VQU28_001465"
     tRNA            complement(20750..20830)
                     /locus_tag="VQU28_001465"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20794..20796),aa:Tyr,seq:gta)
BASE COUNT         6236 a         4735 c         3669 g         6198 t
ORIGIN      
        1 tggtattatc aatcatgaac taggattcta gttttgcagg aagtctaata atgaaaataa
       61 aggactaaag caataactct aaatacatct gatttttata ggatatttca taaaaaaaat
      121 ctacatacct agagtcctat ttagttatgt atcttacata aagagaaagg aattaattat
      181 catgaaatta tctttgtata gtgaagttga acattaccat tttttaagtt cttattcttt
      241 aaatgatatt acatatactg ataccccaaa aaatattatt gaaaagtcta aaaaagataa
      301 gtatagatac ccaatagtaa tacttaataa agaccataaa gtaattggat ttttttgcct
      361 acacctaaac actggtccta aagagtatgg ctattatcaa aatgattatg cgcttattag
      421 aggattttct attcatgata atttcagaaa tcaaggatat ggttctaatg ctctgaatga
      481 aatatttgaa ttcatagact caactttaaa attagaaatt aaccatatta tattggctgt
      541 aaatgaaaaa aatatattgg ctcaaaaagc atatagaaat tccggttttt ctgttgtgaa
      601 aaaagattta gaaggaaaaa gggggaaatt aattattatg gagaaaagca gaaactagta
      661 atgatactag tttctgcttt tctctaagtc tgctataaat ttataacatg ttgaaataag
      721 atgtgaataa attgattgca gaattcaaac caatttctaa caatgtttta gaaaactcgg
      781 cggactattc cagattcaat ccactatatt ctatcttatt tgcatatttt gagagaaata
      841 atctatatac taaactttta tgtttataat tcattccttt cgtttcactc aaggcacaag
      901 acagaatgaa aaagtggtgt gctctttatt ttgttttata ataatagtga gaaaacctat
      961 cactactaca aatcacgggg aggtgaataa gtgagtggta tagccactac ctcgcatttt
     1021 tataggtgtc attctttcca tcaagcacaa gaactgtgac taggagcacg taaacttata
     1081 attgaataag cgactcgttt gtgaaaggaa agaatagtca agggataggc agactcaaat
     1141 ttggtgaagg gaaacccctc atgttaaaag ccaaacttca attttaaaaa ttttaaaatt
     1201 tgtttcgtat tcactactcc ttgcctttat attggattga ttaagagaac ctttcgcgtt
     1261 tctagaaact acagtggact attctggatt caacgcatta tagttgaatt gactaggaat
     1321 tctctttctt ttcaggcttc tcttctgatt tttttctccg ctttttcgct tgaatcagtc
     1381 gctacctctt cctttttatc cgttttctct tctttgattt tgactgaagg aaacagaaac
     1441 tcatattgac tcttatccgt acgaacagtc gtcaccaagc ttgttttctt gttttgatag
     1501 ctcacctgac ctagattaaa aacctgttcc ccactttctt gctcggcttt atccttggtt
     1561 cgtttagcca tggcaaaagc aaccaatttt tctttattta aagcatagtc ttgatagtgg
     1621 gcgatttgtc gggtcaaata aaattgcaac aaaagactaa agatggctgc tatggtgact
     1681 gcgtagagga gaacacctgc cttaactttc ttctttttcc acacgataga tgaactccct
     1741 ttctaagcct ttttgaaact ggaaacgaaa atgaaccagt tgattgtcct ctgtaatctg
     1801 agctgatttg aggccataaa ccataggctg atacccccgt ccactagcat cggttttacg
     1861 aaaatcgtct gactttgact taccgatggc gatgtccttg tcatcttgct tcatgtatag
     1921 gcgattgcct tctacttttt cgaactgcga acggtctaat tctgcctcaa gttggcccac
     1981 aaacaagagc cactcctttt gctcgctttg ttgctggtag cgaacttctg aaatgaggag
     2041 ctgactcata gcttgaaaaa ggagtaatcc cccactgatg acaatgaggg caagcaggga
     2101 ttctaacaag gtaaaagcct tgaccttatg gctctttgat tgccaacaac tgttctgaac
     2161 catggtagac ctccaatccc ttttcactag aaaatacctg aatctcaact ccgttgatgc
     2221 ttacctgatt ttgccccgtc tgcaaggcca tcttagctac cctcaagact tcttcctttt
     2281 gcaagatttt tgcttcctct tgcctatttt tttgaatttg tcccaaaagg agggtcgcaa
     2341 tgctggcaaa gatagctaga gcgactactg cttccagtaa aatcactgcc ctaatttttt
     2401 gtttccttaa tgcgtttaat ttttccattt cctagatata attgatagcg aatcgctcct
     2461 ttactggtct gaaattcaac cttagccagg gacgaattgc ccccagctcg gtcaaatgta
     2521 atactttggc ctgatggtgc ctgaattcct ttaggaactg tcaacttttg actgccattg
     2581 ctaatcatct gcccatccaa gttcagacta gtcttttgtt gactagctac actgcgtttt
     2641 tgggtttccc gatagagttc ttcaaactcc ataaagaaaa tctgttcctc taccgctgca
     2701 aaagtggact ggacagagcc ggacaagccc aaggcaagga tactcacaag tcccaaaacc
     2761 aagagacttt ccagcatggt aaaggcctta atcattgact ttacgatttg cccctccatt
     2821 tttagtatag tattcattat aagctttaac ctgttcttcc gtgatgcgcc catctccttg
     2881 taacttgctc agagtagcat cttcattttt atccaagcta taaagttctg cctggctttc
     2941 caccacctta acaacagctg cttttccttt gtcattgact gcttcttttt gcttggtcag
     3001 attaggtaca aagagcaaga aaagcacgct gataatcagc aagaccacca acatctccac
     3061 caatgtaaaa gctttaacct tagctttttt caagaatgtc atcatttttt tcattttaaa
     3121 aatttacctc catattttga tacatgggca tgagcattgc cgcataaagt aaaacgataa
     3181 tcagtgccac aaagataaaa accagtggct gcaccaaatt catggtgcgg ttgactcggg
     3241 taaaaaaggc ttcccaagtt ttttcagcat agatttccaa ctcactaccc agcttggact
     3301 taacttcccc atactctatg atgagactca attccttcct aaagaaagga taggttccta
     3361 tcgtctgaga aaattcacgg ccatttttca gggtttgagc cagatcttga ccgatttctt
     3421 taaagagctg ggaaccttgt tcctgcatca tttgaaaaat ctgcgtcagc tccattccct
     3481 gtgaaatcat attcccccat tcacgtgcat aataggctgt caagtaggtc tgcacaaaga
     3541 ttccaataaa gggaaggcgt gctaagatag aaaagacact catcttagaa cttcttttat
     3601 aaaaagttag tgctaaaagg gcaagcacgg aaacaagccc taccatgcct agaaaaattt
     3661 gtggcagatt accgataatt cgggtggcaa tattgctact atccagttgt gggagcaggt
     3721 aattccgtag ccccagcata attaagagaa gaaaatccag caaaatcaaa ggataggtcg
     3781 ctacttcaat taactttttc ttgaccttgg ccagattgtc tagatattct tctatctttc
     3841 ccaaactcag gtgaagattg ccatgaactt cagctagaga taactgagtg acaatggtac
     3901 ttgaacatcc caaactttcc atcatttctg agaatgattt cccctgagac aagcctgtac
     3961 gcatctgggt cacacactgc ttgtccaaca aggaactcct atctaaaaag gagatagtct
     4021 ccaccagatg aaaaccgcta gaaaagagat tgttaaatag ggtgatgata tttttttgct
     4081 tagctgtagc taattttttc cgtctcagcc tgaagacttg tgatatgtcc atctttaaga
     4141 agttggtcaa tttgctcatt ccacttggct gcttggtgtt cttgataatc tctgtttgca
     4201 aagtcaacga ttcctcctcc cccgattaat ctctggtagc agactccttg cagaacaact
     4261 gccaattctt cttcactcac acccaactcc agcagacgct cataaacacc tcggatactc
     4321 ttggcgtgaa tggttgaaaa gactgtcgca cctgtcaaac tagctctgac cactgcacgc
     4381 gccgtctcgc tgtcacgaat ttctccgata atcaagagat ctggtcgatg acgcaaggaa
     4441 agtttgatta gattttcata ggttaggccg attgcttcgt tcaactgcaa ctgaagcatg
     4501 tcgtcctgct tgatttcgac aggatcttcg atggacataa cttgctgtcc tttaaagagt
     4561 gacttggaca attcatgcat caaggtcgtc ttaccactcc caaccgggcc agcaaaaaga
     4621 tagagtcccc gttgcctgta ctgcttgcct aattcttcaa tatcctgaaa ccaaaaatgc
     4681 aagtcctgct cctcatcgtg caacaaacgg ataaccaaac tctcatgccc ccgataatcg
     4741 cctacagtag ataaacgtag agacgccatc ttctggtcat aggcataatc acaggaaccc
     4801 agttgactac gtcttttttc tcccacattc atacccgcca caaacttaaa gtgactgata
     4861 acggctgcaa acttttcaaa atcatagcta ccaattttac agcgctcgtc tcctaccctc
     4921 atatgaagct cataggcgtc taacttaggg acaaaataaa tatcctgcgt cccttttttc
     4981 cgagctgaac gaatgatttc ttgtgcaatt tcttgaacca tacttacctc ctcacctata
     5041 ctattcgcaa agcgttagaa aaataaaaaa agcaactcca aaaaagttac ttttctctat
     5101 tttaatttca tacggcaaga acgattcctt tcctgactgg tttgtttttc atagcctgag
     5161 cgtagttttt cgtctgggat aacctgctca tcctgcaaaa gagccaaaga atggtattgt
     5221 tgtaaatact catcacattc atcacaccag cgttggtctt ccgggtccca aaaaatggtc
     5281 atcaagtcgc ttctactttt ataaagaggg gattcttgtt ccaatctaaa ccagcaagtt
     5341 ttgtagtatt tgagagcatc ataatactgg tcaaattttc tacttgctac aatatcttct
     5401 tcccaacctt ctaagaacca ccacggttca aaatctccgt acatttctat aacacgatac
     5461 atattcattc attcctttgt accgtatatt ataaccaatt ccgccagaca aggaaagaaa
     5521 tatgctcatt tcttaatttt ttgataaaaa atccagccat ctgatggatg actgaatttc
     5581 tatttgctta tgtacaaaaa tatttttact acttaggctt ctgagatatc gttttcgata
     5641 atgaccttaa tggcatggtg gtctgctgcc ttactgaaga cttcgtaggc tttttcaatt
     5701 tcactgagtt tgaaatagtg agttaccaat ttttccggtt caatcttatg actttcaagt
     5761 gctttcaaca attgtggagt cgtatttgta gataccaaac cagttgttac attgatgttg
     5821 cgaatccaaa gtttatctaa atcgaattca actggtttac catgcacacc acagttggca
     5881 accgttccgt ctacaccgat aatcttttga cagaaatcaa atgtttcagg aatccctaca
     5941 gcttcgatag cgacatccac accacgacca tctgtcaaat cataaatttc tttaatggct
     6001 tttttagggt ctgaagaatt aaccttatga gtcgcaccga atgatagggc agtttccaag
     6061 cggttatcgt ctaggtctac cataatcaat ttagctggtg aatagaattg ggctgttaaa
     6121 agagcagcca atccaactgg acctgaacca ataatggcta cgctgcaacc aggttctact
     6181 ttccctttta agacaccaat ttcatatcca gtaggcagaa tgtctgacag cataaccaaa
     6241 gcttcatctg acaagtcttc tggagtatgg taaagagtat tatctgcatg agggacacgt
     6301 agatattcag cctgcatacc atcaatcaag tgaccgaaaa tccagccccc ttcgtcttca
     6361 cagtgggcat aaattccttt tttacagtag tagcatttac cacaggcaca gacgcaagaa
     6421 atcaagacct tgtcaccttt tttgaagttg gaaactcctt ccccaacttc ttcaacaatc
     6481 ccaatccctt cgtggccaag aatggtacca ctttggcaag taggaacatc ccctttgata
     6541 atatggaggt ctgttccaca aatagtggtt tttacaatac gcacaatagc gtctgttggc
     6601 ttgcgaataa ctggtttgtc tacatcaaca aaagaagcaa gtcctggttt aacataagta
     6661 taggctttca taaagcactc ctccgttttt ttatttgtaa tatttatagt tcaattgtaa
     6721 gcctttttct ttgtttcttc aagctttttt tgtgattttt ttaacttttt atttaccagt
     6781 aaagtaccct gtataaaaaa gcagaagcta gtctctaact tctgccttct ctcttatgct
     6841 tgataacgtt tcacaccatc taggtaggtt gctaccaatt ccaaatcttt atctaatacg
     6901 atgaagtcag cgtcgtagcc ttcacggatt tggccacaga cgtcatcgat gtgaacggat
     6961 ttagctgggt tgaagctggc catcatgact gcttcatgcg gattcgcaat tccccattcg
     7021 accacattct tcaaaccatc tttgagtttg aggatagaac ctgccaagtt acctgtcgat
     7081 ttgaggcgtg cagttccatt tgcaacgaca actgggaatt ctcccaacat ataatctccg
     7141 tcttccaatc ccccagctgt catacagtct gtgataagag cgatgttttc tgttcctttt
     7201 tgtttgataa gaatttcgca agcctttgga tctacgtggt gaccatcaca gatcaactct
     7261 gcataggtat gtggcaattg gtacatggct ccaaccatac ccaattcacg gtgagtcaac
     7321 ccacgcattc cattgtaggc atgcacccaa acactcgctc cagcatcgac tgcttttttg
     7381 gcttcatcaa aagtcgcgtt tgaatgtcca agagcaaccg tcacaccttc gcccgtaact
     7441 gtacgaacaa agtcttccac cccatcacgt tctggtgcaa gggcaatttt attaagcaag
     7501 ccatttgccg ctttttgcca agaatgaaac tcctcaacac ccgggtctct catataagtt
     7561 ggattttgtg cccccttaaa agtttctgtg aaatatggac cttcataata aatcccacga
     7621 atcttagcac ctgttgcttc tttataatgg tttccaagat tttcagtgac tgcaagcaat
     7681 tgctcataag tggctgttaa agttgtgggt aagaaactgg taacaccggt actaagaagt
     7741 ccttcactca tagtatgcaa tgtaccttca atgttgttgt ccatcacatc tacacctgca
     7801 tatccatgaa tatgagtatc cacaagtcct ggggcaatgc tataacctgt atagtcaatc
     7861 acctcagccc cttcaggaat ctgctctaca tgtttcccaa acttgccgtc cacaagttcc
     7921 aagtaaccac ctcgacgaac tccgtgtggg tagaaaaact gatccgcttt aatatagtta
     7981 ggcataatgt taacctcctt aaaagattga ttctacaatt tattatgtca attacattat
     8041 aaacctttct tttttatttg taaatggtat agacctattt tttggagata ttttaaaaga
     8101 gctggatttc tccaactctt ctctttttta atacaagtta ttttgattta accggcttgt
     8161 cttgagctgt ctgcaaagct gtggcaatcg tatctgcata caattttgct cctgcttcga
     8221 tagtgctact ctcactcccg aaatgaacct ggtctgttcc agcccaaatt tctggatgct
     8281 ctttcgcaac ttgattccaa tctgctatcg taatgtaagg tgtcttctct gccaattctc
     8341 tcatataggc agcagccttc tcaacgatgg cataggtctc ttttgtctta tctccctcat
     8401 aaggagtcac caaaatcata tggtgtccct taggaagatt tttcacgata ctgtcccagt
     8461 catccttgta attctcagga ttatttaccc cagtcgcaat gaccaccgtc ttaggtaaaa
     8521 atttattctg gctattattt agcatgattt catttgcggt cttggttgtt acgctgacct
     8581 gcgcgttaat ctgtgctcca ggaagagctg tctgtagtgc tgtatttgcc cttaaagcca
     8641 ctgagtcacc aattaacata gtgccatcag caattcccaa actgtttgca tctgcccgtt
     8701 ctgccatcac cttggtctgg ccaatatttg ttgcagcttg cttcaagcca ttgacagtca
     8761 agtctgtctc aaacgctccc acttgtggtg ccaacaaggt caccatgcag acaatgaagg
     8821 tcaagattcc tgtacctgct gcaagaattg cgtgaatata aggcagggga cgaagagttt
     8881 gtaaaatagg tgtgttcttg cctgcaatcc aaggttccaa tacataaaat gacagactgg
     8941 caaagccata agaacaaatc agagtcagta atacagcaag aagatttgat gtcaactgtg
     9001 agaaaatgat atagaaaggc caatggaaaa gataaaccgc atagctagta tccgctaaaa
     9061 agctgataat cttcggctcc tgtatatgat gtgtcttttc atgtaagaca cgcgccgcca
     9121 gaatcatggc aagagctgca agactggcaa gcaagaagcc gataagatag gcaaaaagat
     9181 aagtgaattt gacaaagaaa gtcaaaagaa ctaggaaacc aaagcctcct ccaaaaacta
     9241 ctaaagtctt tcgtaaatcc cagattttat ccaactgctt gacgagggaa gtcgtctgac
     9301 gaacgcctac aatagttgct aacatacttc ccaaaaagaa tggatagaca tgagttaaac
     9361 tggagaaata aacagaggaa taagaggtca ctagaaaact accaataaac atggagaaga
     9421 aactgatcaa gaaggcagta gcagataaga gaaagatcat ccccttcaac tgaccatttg
     9481 atttagcttg tttggataag aaccaaactg ccaatcccca aagaatatag tagtgaacct
     9541 caactgccaa actccaatta tgaacaaaca aatgaggaat gaactgagat tcataactcc
     9601 cacctgttaa gagttcatag aagttggtca taaagcctaa gacgcccgca atctggccac
     9661 caattccagc aacatagtct tggcgaacca agaaagtaaa aggcatggtc accaagacca
     9721 tcaaaaccac aggtggcaca atccgataaa agcgtcttct aaaaaatcct atcaaatcaa
     9781 tctcattgtt cttagaaaat tcttcaatga gtagagctgt aatcaggaaa cctgaaaatg
     9841 tgaaaaagac atctaccccg aaaaatcctc caggaaagat cgtctgaaag aagtgataca
     9901 agagtaccaa aagtaaacct ataatcctaa tcaaggaaaa ccatttaatg cgcatacgag
     9961 tttattcttc ctttcattgt acactttatt ctatcaaaaa aagaagaaaa atcctaagag
    10021 aaaacttagg atttttagct tatatcaatt ccattttaga aattacgtcc tgacttatta
    10081 tagccatatt tttccacaaa atacttacgg aattccaaga gattgtcatc catgatggct
    10141 tgtcgcactt gcttcatcag gttaagcaag aagtaaagat tgtggtagct agtcaagcgg
    10201 ataccaaagg tttcatcagc cttgagcagg tgacgaaggt aagcgcgtgt atagttatta
    10261 catgtgtagc aatcacactc aggatccagt ggcgtaaagt cctcagcaaa ctgggcattt
    10321 ttcacaacca aacgtccttg actggtcata caagtcccgt tacgagcaat tcgagtcggt
    10381 aagacacagt caaacatatc caccccacga atgaccccat cgatcaagct atctggcgct
    10441 cccacaccca tcagataacg aggtttattt tcaggcagca gttgagttgt aaagtccaag
    10501 accgcattca tctcttcatg ggtttctccc actgccaaac caccgataga gtagcctgag
    10561 aaatccatgc tgacaagatc gtgagctgat tggcggcgaa ggtcttcaaa tcctgcacct
    10621 tgcacaattc caaacaaacc ttggtcatgt ggacgacggt gagccttcaa accacactca
    10681 gcccaacggc tggtacgctc gatcgatttc ttaacgtagt cataaggttg ataaaactga
    10741 ggacattcat caaaggacat catgatgtct gaacccagat tattctgaat agagatggct
    10801 ttttctgggg ataggagcat cttagaacca tttagatgat ttttaaaggt tactccttct
    10861 tctgtgatat tacggctatc tgctaaagaa taaacctgaa aaccaccact atctgtcaag
    10921 ataggctggt cccaattcat gaacttgtgg agaccaccag cgcgtgcaat gagttcatct
    10981 ccagggcgaa gccagagatg ataggtgttt gataggataa ttcccgaacc catctccttc
    11041 aattcttcag gtgactgagt tttgacagtg gcttgtgtcc caactggcat aaacataggt
    11101 gtcggaaagg taccgtgggg agtgatgatt tctcctagac gagctcctgt gtgtttttct
    11161 ttcttaatca aacgatattt gattggtgaa tctgacattt tttacctccg aagctgggaa
    11221 agacagtccc agttcataca gtgtgcccaa ggacacactg tatgatttta tcaaaaaaag
    11281 taggaactgt cacgaactat gtataagaac tatggtataa taagagaatg aactatccaa
    11341 aaattgattt aaaaacaatt cgtcaggagt caaaacattt tcaggctgac accccccgtc
    11401 tctttctcct ctatatcctt cccagtatgc tggtcatctt atctggtttt cttaatccct
    11461 tatctcggat ccacgggact gttttagaac agccattttt cagcatactt ggccaaatac
    11521 ttcaaaccta cctctttcct ctactagtct cctttatcgg gactattctt ttgaccagtt
    11581 ccgtctatgc aacactgaca ctcatgaaag attctaaaac ggagccatct gtaaaaaaca
    11641 gcctagctct ctttgatgag gagcgctttt ctcaaacctt tttgacccta cttctcaaac
    11701 gtttctatct ctttttatgg agtatcccta atttacttgg gatttacctc ctcttttata
    11761 gtagcttttt agccaaaaaa ttcgtcaccc tacatcctga gtttccaaat ctagatctct
    11821 catcagttga aaccgagcgt ttcctcatgg tctttggact ctactttctt gcaagtctca
    11881 tcttgatcat tgtaggaaat atcctctata ttccacagta ttatgcctat tcacaggtgg
    11941 aatttcttct ctgttatagt ctagacctag gacaggtccc accaagacga atcctaaaaa
    12001 ctagccgttc ctttatgaag ggctacaagt tccagcactt tgtcctagat ttacagcttc
    12061 ttccttggta cttcctcaat tggattacct ttggcattgc tagtttttca ctcctacctt
    12121 atatccaatg cactaaaatt atgttttacc gagcagtatt ggctcgaaaa cgtccaaaag
    12181 cttgaaggtt tttcccctca agcttttttc tatcaatgag tttctccgcc taccaacttg
    12241 agttcctgat ctccatgttc tatcatagcg ccgattgctg cttctatccc tcgccgaata
    12301 tccactaaac tcatagttgg agtagtcggt ctgttcacca cctgttccat catataagga
    12361 atatgcataa aacctgcctt aacatatgga aatttctttt ctaccaaata gagagcctga
    12421 tacatcaaat ggctgcagac aaaagtccct gccgtattgg aaacagaggc cggtaagccc
    12481 tcttttttta tagcttgaac catcgcttta atcggcaaac tactaaagta ggccgaagca
    12541 ccatctgggc gaatgggacg gtcaatcggt tgattatctt cgttatcaga aatgcgtgca
    12601 tcgtcttgat taatggccac tcgttcaggt gtcacactag ttcttccacc agcttgccca
    12661 atacaaagga caaagtcagg ttgataacga ttcatctctt cttccaatac ttgagcagat
    12721 ttgtgaaaaa ctgtcggcac ctctagccaa cggacctcag caccatggat ttcagctggt
    12781 aaacctttaa tggcctccaa agttggattg cccttttccc ctccaaaggg ctcaaaacct
    12841 gtcactaata ctttcatttc taactcctca aaaccaattc aatgagactt ttttcttaaa
    12901 aacaagtata acatatttcc cttattctgg attaaaaagg attagcgaga ttaaaatcga
    12961 ccttcaagtg tatttaaaga gtaaaatact aatcaaggcc aaaatagctg atccaccttg
    13021 cttcaaaata atttttttat ccgctgttaa agagccgtaa gtcgcagcac caatcacaaa
    13081 taagacaaaa atagtcacaa tttctaaatt ctgtgagaaa taaatgccat accagagaaa
    13141 gactcctagc agagccttat aaatcccttg atttttgaac aatgaactta ctgacggatg
    13201 agccaattct tccttttcca tattaaatac acgactagtc gcatctgatc gcgtagcaat
    13261 actttccaaa taaaaaatgt aaaaatgctc caaagcaacg atcgttgtta aaataattgt
    13321 aataattgac atctatttct ccttaaattt ctatattttc aatacccaaa accaatttat
    13381 ttaataaacg gctgagttct gttctctcaa actctgttaa gatactttcc atctcttcct
    13441 taaccttgat atgttgcaga gggggattca ccactaactg ctccttggca tacttcgcag
    13501 cctctaccaa cacttcccgc tgattttcag gattacgatg acgctccacc aaaccttccg
    13561 tttccaaaat cttgaaatgc cgtgtcaaag cagcctgatc aattttcaaa cgctcctgaa
    13621 ccgccatttg gttacaagga gaatgctcca gcaaaaacag taaaatctga taccgtgtca
    13681 aactaatccc aagccttttt tcaaacaatt gcgtactcgc ttgctcagac aagcggagtt
    13741 gatacagtaa atcttgaatc tctatcattt cttcctccta tccattgact tatcaatagt
    13801 tgaccaatca attataatat aaagtatagt gaaatgaaat aagagcagga caaatcgatc
    13861 aggacagtca aatcgatttc taacaatgtt ttagaagcag ttgtgtacta ttctagtttc
    13921 aatctactat aaaatagatt gcaagaatgt tgcttagatt attacaagat gataactttc
    13981 cattttccat tcaaattgat actcaatgaa aatcaaaaag caaactagga agctagtcgc
    14041 aggttgctca aagcacagct ttgaggtttc agataaaact gacgtggttt gaagagattt
    14101 tcgaagagta tgacaaagaa aaaacgaacg agaatcgctt cccatcacga aagctagatc
    14161 tcattcgtca aaatgatgtt accaagtcgg gtttatccat tcattgaaac gtgaacgtga
    14221 tcatagtgat tttctgtcac actaccacgg tctggcattg ggttccaagt gttagctggc
    14281 ccatatttgc tatcgaatgg agcatagaaa cgttgtttcc agatgatgta actaatgcca
    14341 cggctggcca tgttttgaat agcatattcc gcaatcttat cccctaattc tgaacgttct
    14401 ggtaccataa agtcgatagc caaacctttt ccgtgatctc cactgtctcc tggacgataa
    14461 ccgctaaagg atgtaatgcc aaacaagtta gcaatttctt ctttaaaggc agctgtttgt
    14521 ggttgaagac ctgcattttc agattttgct actgcaagtc cagcataatc gggcgcagct
    14581 ggagcctcgt aagttgttga aattattttc gtctcttctg gttgataagt agaaactgct
    14641 gctgtagctc catttgatga tgctacttct tttgcttctg aacttgttgt agttgcttcc
    14701 actgctgatg cagcttgagg gcttgcttgt gtttcctgct tctcagctgg agtcgttccc
    14761 tgaggagctt cttctgcaac tgcactgctt gtttcggccg tttgctcctc tgggacagaa
    14821 gtgcccgtag atggtgccac ttcttcagaa gcaatcactg tctttgtaac ttctgaactt
    14881 gatgctactt cttttggagc ttctgcaatt ggttgagaaa ggtctgcaac ctgaacagtt
    14941 tgatcatcaa cggtcacttg attagtggtc aaatctgctg tcgcagttgt cacttcttca
    15001 ctagagtctg cttgaggtgt ttggatttca acttctgtta cttcttctgc ttcattgaca
    15061 gtcgttgtca aaacagtttc tgggaaaatc aagtccatat tagtgatttt gttcagattc
    15121 gcaagcactg tgacatctac acccaaggct tctgcaatgg tgctcaaagt atcaccatac
    15181 tgtacggtat aacttgtttt gttgtccgtt ttagtcaaat cgttttggat ttgctcaaca
    15241 ctacgtgcag tccaaagaac ttcttctgct tgagttgcca atactggggc aaatgacaag
    15301 gctactgttg acgctaataa cattcttttc ttcattcttt caaattcctt tcaaatgagt
    15361 acctgtctat cataacacaa aaaatgtaga aaaaaagccc tctcgggcct atttaacaga
    15421 actgtctatt ccttgtaaca aactagatta cttgaaatag tttgttaggt ctctgtcaca
    15481 aaactgacac aatcttttta tcacttagtc tcaaaaatac gggaaatatc tgctaacgct
    15541 tgaatcctgt gattcccttc ataagtagac tctaaaaagt tgatactttg aatcccacta
    15601 ttctgggcaa attccacatc cagagtccga tcccctatat aataagtatt atcagaattc
    15661 aactgatact tatctagcag ataggtagcc gcttctggac ttggcttccg cacaaagcca
    15721 ctctgactgg tcaaaatctc tgtaaaatag gattccaccc ccaagtcctt gagaatggta
    15781 aaagtgttgt tccccttatg agtatatata aactgctgaa ttcctgattc gtctgcccaa
    15841 gctagcacct cacgcgcacc tggcatcaaa actacctgag cattcttctc agccagactc
    15901 tgggcacgca cctgatttag cacctcaaca tccagatttc tatcttctgc cacccgcaca
    15961 agcaaatttt gcaccgaata cttgaagata aactctctca ccttctcctt atcataagga
    16021 atagaaaact gagcaaaagt ctcctcaatc cctgataaaa tcgcttcgta agagtccaat
    16081 aaagtcccgt ctaaatccca aataaaagct gttttttgca tttcctatcc tttcaagctc
    16141 atatagcgct ggtaacgata gcgtagaaat aaccagcgaa aaccattatc caagagagac
    16201 cctgcccaaa taccaggcaa gccccaacca agcacaatac ccatcagata tcctgtccca
    16261 atgcggatac accacatgcc tatacttgtc gcataaaaag ggaggcgtgc atttcctaat
    16321 ccctgccaga ctgccgtata gatgactgtt cctgtcgtca taggggtacc aagtagtgaa
    16381 aacagtgtca ctagaacact agcctccacc gctagagaat cagtcgtata gagatgagtt
    16441 aatggtacac ccaagacata tatactaaag gacaggggca acatgaggaa cagagaaagc
    16501 caaaaggttt gtttactcaa actagcaact cttttccaat catcctctcc aactgctcgg
    16561 gccaacagca tgaccgttgc cgtagcgaca ccaaaggcag gcatatagtt aaactgggtc
    16621 aagacttctc cgattgcatt cccagcaact gcctccgtcc caaaagaaac gaccaaggca
    16681 atgatcacta catctccagc cctcatcata agtcgctctc cagctgctgg taaagccaag
    16741 gtcaacagtt ccttatctaa accaaaagtt agcttcccat aaggcagttt taattgtgac
    16801 cacaaaatca caagaccaac caaacgagac agaattgtcc cccaagcaac accagctatc
    16861 cccatatcca gaacaaaaat agctagactt gaaaaaagaa tattcaaggc attggataaa
    16921 aaactaacat agagaggcag acgtggatta tgcgttgcac gaatcaaggc tcccaaacta
    16981 gtcattaaac ctaagagaac aatcgatccg cctaccaaag atagatacag tccaccactc
    17041 tcagctacat ccctctccgt ccccaaaagt cctatcatct ctttcccagc gaagatggac
    17101 aaaaatccta aaaggaaact taatagtaag gtaatcttca acgcctcagt cacatgatag
    17161 gccaacttcg actggtcttt ctgccctatg ctttttgaaa taacactgga aatagcagct
    17221 cccagagcga tgaaaatcgc ctgataaatg gtgataatat taccagctac tgaaacccct
    17281 gaaatagcta tcaatcctaa atgagcaacc aaataactgt ccaccattcc cattagcatc
    17341 tgcaaaaagt tttcacccat agctggcaat gcaatattaa gaatgtcttt atttttctta
    17401 aacaatctct cctcctgatg aaaagaaact cagttggttt cccaaccgag tttgctccct
    17461 ctgtcttaaa gtcctaaata agcctcaacc gctgcttgca tgtcagcagc tgccactgtt
    17521 gtcttgtgac gaattggagc tatttcaagc ccatcaactg ctggtggcac tgcaacgcct
    17581 gagatttcat gtaattgagc caaggcttca aagtctgtca aacctgcttt tccagttaca
    17641 gcttctactg caactactgg gaacttgtat ggactagctg ttgaagcaat cactgtctta
    17701 gttacatctc cagtaaccgc ttggtatttt ctataaactg ctgaggcaac cgccgtatgt
    17761 ggatcctcaa tataagaatc tgactcataa acacgcttga tttctgccgc tgtttcttcc
    17821 tcagtcgcat attcagctgc aaagaggtcc aaaatctctg catcaaagtc tgtcaactta
    17881 tattgtcctt gcgtgttcaa ggcattcata agttcagctg tcttttcagc attatttccc
    17941 aaaagatgga aaatcaagcg ctccaagttt gaagatacca agatatccat agatgggctg
    18001 gttgttacct taaactcacg ttttttgtca tagacacgtg ttttaaagaa gtctgtcaag
    18061 acattattgt catttgaagc acagattagc ttgccaactg gtaggccgat ttgtttggca
    18121 taaaaggcag ccaagatatt tccaaagttt cctgttggta ctgtgaagtt aaccttttca
    18181 ccagctacaa tttcaccagt cttaaccaat tgagcgtaag cataaacata ataaacaatt
    18241 tgtggcacca gacgaccaat gttcatagag ttagctgatg aaaattgcaa cttgttggta
    18301 gtcaattttt cacgaagagc cacgtcgtta aacatgtgct tcacatttgt ttgcgcatcg
    18361 tcaaaattac catcaatagc aataacatga gtattgtcac cattctgagt ggtcatttgc
    18421 aactcttgta ccttgctgac accacccttt ggataaaaga cgataatctc agtaccaggc
    18481 acatccgcaa accccgccat agcagctttc cccgtgtcac cagatgtcgc tgtcaagata
    18541 acaatcttgt tctccaaacc atgtttctta gcagcagtcg tcataaagta tggcaaaata
    18601 gacaaggcca tatccttaaa ggcaatcgtt gaaccatgga aaagttccaa attgtattgc
    18661 ccatctaatt tcactaatgg tgcaatagct ggagtatcaa atttgctatc gtaggcattg
    18721 ttgatacagt agtccaactc ctcagctgta aagtcatcta aaaatgctga caaaactagc
    18781 ttagcaactt cctggtaaga agcatctttc aatttgtcaa agtccaaatc tacctttgga
    18841 taagtaaccg gtgtaaacaa accgccgtcc gtcgccaaac cttgcaaaat tgcttggctg
    18901 gcagttactg tattgttggc atcacgcgtt gattgataaa ctaatgtcat tactctctat
    18961 cctttatcta tagtctcgtc cattatatca tgatttttca gaaaattctc cctttataag
    19021 agaaattata ggctcaattc ttctctcaaa ggctgtaaat aggtcatttc ttccttattt
    19081 ctcaccacta gtccctcctc agctagtagg agtaagtctt tggaaaattc aataatcgta
    19141 gtttcttcct catctgtaag atttttctta gaaaattgtc tccttaaaga cttgtaatca
    19201 taaccaaata ctttaaagaa aggtgctgtt tctaagtaag cttctaactt gtctaaatta
    19261 accaataatc ccaagtgaaa agctgcagaa gcaaaagtcc tatcaagtgg ctgtgtacac
    19321 acactacgaa actcaactgt tcctcgagtc gttaaatctt ggtactggta actacgatga
    19381 gtttcaaaat ccttctcttg ggggtaaata ataacctcat ccccattcag agcaaatgct
    19441 tggatttcgg acgtagccaa atagtcccca gcctgaatag gataaaaata ataggtctgc
    19501 ccatcacgtt ccgcagtaaa aatcgcagaa tgatttagat agtcaaaaaa atcagcttca
    19561 tcattaagga gtctagcatt gaccccaaca ttctctggat agataccatg catagattct
    19621 tcccagaaaa tatcccttga aattttcgta tcccaatccg cacccgaaaa ttcagagttt
    19681 gcaaataaat aagccttagc cgcttcaatt tgagtaaaag cattaatcac ccgtaagtag
    19741 ttggtttttg aaatatccag ctgaacctgg ctcccacaga taaaagtacc atattcaggg
    19801 aaatgatgta aatctgattt aataatattt ctactcaaat tcaaataatc catcaacatc
    19861 tgatagcgtg gataagccac tggacaattc tcatttttat cccagttggg atggatacca
    19921 cagccaacaa tagcatgatt tgattcagct aactttctct gaattacatt catataatta
    19981 ttgaaacgat tttcgacctc ttgaatcgtt tcagccttac caaatgcaaa ctcaatcgtt
    20041 gtataggaaa cttcaaataa aatagcatcc tgacttatcg gatctactaa ctggatcaga
    20101 ttgccaaaat catctacctt tgctacggtg agatccaaag tagaaactaa ataatgaaat
    20161 agatccttta taacttcaac atctgtagca tccccttcta aacttgcaac aggatactcc
    20221 aactcaattc cgacaaacaa ttcaggattt tctttaatat ttttcaaata ccgatgtttt
    20281 aataaatcga tagaacaaga catactaccc tacactttct taatctaaat aaaatttgaa
    20341 taactataat tataccaaaa ataatataaa aaaggaacga agaataacaa cgttcctaac
    20401 actatactat accggcggcc ggggtcgaac cggcacgtcc ttgcggacac tggattttga
    20461 gtccagcgcg tctgccaatt ccgccacgcc ggcaaatagt aactggggta gctggattcg
    20521 aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaataata
    20581 taaaataggc gagtgatggg gatcgaaccc acgcatgcca gagccacaat ctggtgtgtt
    20641 aaccacttca ccacacccgc cataattcta ttaacacggg cagtaggaat tgaacccaca
    20701 ctgaaggttt tggagacctt agttctacct ttaaactatg cccgtaaaat ggaaggggag
    20761 ggattcgaac ccccgaaccc gaaggagcgg atttacagtc cgccgcgttt agcctcttcg
    20821 ctacccttcc aaaatata
//
LOCUS       DAVBCB010000042        20780 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVBCB010000042 DAVBCB010000000
VERSION     DAVBCB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.042"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            398..1091
                     /locus_tag="VQU28_001466"
                     /pseudo
     CDS             398..1091
                     /locus_tag="VQU28_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            1147..1419
                     /locus_tag="VQU28_001467"
     CDS             1147..1419
                     /locus_tag="VQU28_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase"
                     /protein_id="HET2476805.1"
                     /translation="MFLLFSALVGMLSQLKEVPESVFLQETVEENHLVNVYSVLEVIS
                     TLAFSVFVLLMSYITESFGISISFWLSAICLMIEAILIYIRRDYFK"
     gene            1733..1864
                     /locus_tag="VQU28_001468"
     CDS             1733..1864
                     /locus_tag="VQU28_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HET2476806.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            1885..3435
                     /gene="dltA"
                     /locus_tag="VQU28_001469"
     CDS             1885..3435
                     /gene="dltA"
                     /locus_tag="VQU28_001469"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HET2476807.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFVQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMIMDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGVTHFYFDG
                     EELTVKTAQKLRKRFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            3432..4676
                     /gene="dltB"
                     /locus_tag="VQU28_001470"
     CDS             3432..4676
                     /gene="dltB"
                     /locus_tag="VQU28_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HET2476808.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMWGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            4690..4929
                     /gene="dltC"
                     /locus_tag="VQU28_001471"
     CDS             4690..4929
                     /gene="dltC"
                     /locus_tag="VQU28_001471"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HET2476809.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            4922..6190
                     /gene="dltD"
                     /locus_tag="VQU28_001472"
     CDS             4922..6190
                     /gene="dltD"
                     /locus_tag="VQU28_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HET2476810.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            6345..6785
                     /gene="adcR"
                     /locus_tag="VQU28_001473"
     CDS             6345..6785
                     /gene="adcR"
                     /locus_tag="VQU28_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HET2476811.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            6785..7489
                     /locus_tag="VQU28_001474"
     CDS             6785..7489
                     /locus_tag="VQU28_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HET2476812.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNTHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            7482..8288
                     /locus_tag="VQU28_001475"
     CDS             7482..8288
                     /locus_tag="VQU28_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HET2476813.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGRSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            8298..9803
                     /gene="adcA"
                     /locus_tag="VQU28_001476"
     CDS             8298..9803
                     /gene="adcA"
                     /locus_tag="VQU28_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HET2476814.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKVTFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(10923..11696)
                     /locus_tag="VQU28_001477"
     CDS             complement(10923..11696)
                     /locus_tag="VQU28_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2476815.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            11872..13275
                     /locus_tag="VQU28_001478"
     CDS             11872..13275
                     /locus_tag="VQU28_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HET2476816.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            13390..14028
                     /locus_tag="VQU28_001479"
     CDS             13390..14028
                     /locus_tag="VQU28_001479"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HET2476817.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMELMAEKFKTYGQRK"
     gene            14040..14480
                     /locus_tag="VQU28_001480"
     CDS             14040..14480
                     /locus_tag="VQU28_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HET2476818.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            14473..14904
                     /locus_tag="VQU28_001481"
     CDS             14473..14904
                     /locus_tag="VQU28_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HET2476819.1"
                     /translation="MFNRGILVASHGDFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVDELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISNYDSKINLEEIVHNAQNSLFNVKQQLNVEEEENLCL"
     gene            14895..15365
                     /locus_tag="VQU28_001482"
     CDS             14895..15365
                     /locus_tag="VQU28_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2476820.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            15390..16163
                     /locus_tag="VQU28_001483"
     CDS             15390..16163
                     /locus_tag="VQU28_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HET2476821.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            16160..16960
                     /locus_tag="VQU28_001484"
     CDS             16160..16960
                     /locus_tag="VQU28_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HET2476822.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17019..19313
                     /locus_tag="VQU28_001485"
     CDS             17019..19313
                     /locus_tag="VQU28_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HET2476823.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPVNLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            19323..>20780
                     /locus_tag="VQU28_001486"
     CDS             19323..>20780
                     /locus_tag="VQU28_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476824.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLH"
BASE COUNT         6450 a         3391 c         4377 g         6562 t
ORIGIN      
        1 ctactcgatt aactcccctg aaatttgttt taattatgca aatttcacgt atttttgatg
       61 ctgagacgac gatcctggga acttttcaga tatttttttg actatctaaa atctatcatt
      121 agaaaagctt agagcgccaa aggatttgag cgtttttctg atttttaaga ctttttccca
      181 gtctcttttt cgattgaaga tgtaattatt ctactaacta acttcttagt actagccaac
      241 aacgataatc ataattcctc ctaaaattag gaataataaa ggcaatagtt tttgtttttt
      301 catgtaaaaa acctcacttt tgttttctgc tattttatgc taaaatatta aaaatcaaat
      361 ttaattccaa agtttgtaac taaaggggga gcgctacatg tctaattcat ttgtcaagtt
      421 gttagtctct caattatttg caaatttagc agatattttc tttagagtaa caatcattgc
      481 taacatatac attatttcaa aatcagtaat tgccacatca ctagttccta tcttaatagg
      541 aatatcctct tttgttgcga gtcttttagt tccgttggtt actaaaaggt tagcgctaaa
      601 tagggtttta tctttatctc aatttggaaa gactatatta ttggcgatac tggtaggaat
      661 gtttaccgta atgcaatccg tagcgccttt ggtgacctat ctatttgttg ttgcaatttc
      721 catactagat ggttttgcag cacccgtttc ctatgctatt gtgccacgct atgcgaccga
      781 tttgggtaag gctaattcag ccttatcaat gactggtgaa gctgttcaat tgataggttg
      841 gggattaggt ggactcttgt ttgcaacaat tggtctgtta cctaccacgt ttatcatttt
      901 agtcttgtat atcatttcta gctttctgat gttatttctt cctaacgctg aagtggaggt
      961 gttagagtca gaaactaatc ttgaaatttt gctcaaaggt tggaagttag ttgctagaaa
     1021 tcctagatta agactttttg tatcagcaaa tttattggaa attttttcaa atacgatttg
     1081 ggtttcttcc attttgtagc tatggcaata gtgacactga ctattctata ttttccaaac
     1141 gcacagatgt ttttattatt ttcagcttta gttggtatgt tatcgcaact aaaagaagtt
     1201 cctgaaagtg tatttcttca ggaaacagta gaggaaaatc atttagttaa tgtctattcc
     1261 gttcttgaag tgatttctac attagcattt tcagtatttg ttttgctaat gagctatatt
     1321 actgagagtt ttggtattag catcagtttt tggctatcag ccatttgtct gatgatagaa
     1381 gctattttaa tttatatcag acgagattat tttaaatgag agagtggtta aaaagtcttt
     1441 aaaaatcaga aaagcgcata gtatcaggtg ttgaaaaaac ttgatattgt gtgttttatt
     1501 atgggaagat ttacttcatt gtctcctgaa atggagtttt gaagcaggat cactctattt
     1561 gaaaatatac atagaaaaac tgtataatcc ttaaaataag ctgaaatcta ttggaaacat
     1621 acggtaaaca attgaaaagg tactatataa agtggtacaa tggtagaaaa ataagctagt
     1681 aagaatcact cttattttag cgaaaaatta cagaaatgaa tggtttttct tgatgaaaca
     1741 gagaaaagaa ttgtacctct ttcttggtcg gacagccttg tattttctta tctttctagg
     1801 gctgctttac ttctttagct atcttggtca gggtcaagga agctttatct ataatgaatt
     1861 ttaacttgaa ggagaatccc tattgtgtca aataaaccaa tagcagatat gattgaaacc
     1921 attgagcatt ttgctcagac acagcctagc tatcctgtct ataatgtttt ggggcaggaa
     1981 cacacttatg gcgatttaaa ggctgattcg gatagtttgg ctgcagtcat tgaccaacta
     2041 ggcttgcctg agaagtctcc tgtggttgtt tttggtggcc aagaatatga aatgttggca
     2101 acctttgtag cgctgactaa gtcaggtcat gcctacattc caattgatag ccattcggcc
     2161 ttggagcgag tttcagctat tttagaagta gcagagccaa gcttgattat tgccatttca
     2221 gcctttccct tggagcaggt ttctacacca atgataaatc tagctcaggt tcaagaagcc
     2281 tttgttcaag ggaataacta tgaaatcacg catccagtca agggagatga taattactac
     2341 attatcttta cttctggtac gactggtaag cctaagggag tgcagatttc acatgataat
     2401 ctcctcagct ttacaaactg gatgattatg gataaggaat ttgcgacacc gagtcgtccg
     2461 caaatgctgg cacagccacc ttattctttt gacttgtctg tcatgtattg ggcaccgacc
     2521 ttggcacttg gtggtacgct tttcactctt ccttcagtca tcactcagga ctttaagcaa
     2581 ctctttgcgg ctatcttttc attgccaatc gctatctgga catcaacacc atcctttgca
     2641 gatatggcca tgttgtctga atacttcaac agtgagaaaa tgcctggagt cacgcatttc
     2701 tactttgatg gtgaagaatt gacggtcaaa acagctcaaa aactgcgcaa gcgtttccca
     2761 aatgcccgta tcatcaatgc ttacggccca acagaagcga cagtagctct gtcagcagtt
     2821 gccgtgacag acgagatgtt agcgactctc aaacgcctac caatcggcta taccaaggct
     2881 gattctccaa cctttatcat tgacgaggaa ggaaataaac tgccaaatgg cgaacaggga
     2941 gaaatcattg tttctgggcc agctgtttca aaaggttata tgaacaatcc tgaaaaaaca
     3001 gcagaagcct tctttgagtt tgaaggtctg ccagcctatc acacaggcga tgtgggaacc
     3061 atgacagatg agggcttgct tctctacggc ggacgcatgg acttccagat taagtttaac
     3121 ggttaccgca ttgagttaga agatgtctct caaaacctca acaagtctcg ctttatcgaa
     3181 tctgctgtcg cagtaccgcg ctataacaag gaccacaagg tacaaaatct attggcttat
     3241 gtcatcttaa aagacggtgt tcgtgagcag tttgagcgag atatcgatat taccaaggcc
     3301 atcaaggaag acctgacaga catcatgatg tcctatatga tgccatctaa attcctttac
     3361 cgagacagtt tgccactgac tccaaatgga aagattgaca tcaaaggatt gattaacgag
     3421 gtgaataaga gatgatggag ttttttcaac agcttcctca tttagagcca tatggcaatc
     3481 ctcagtattt tgtttatgtg attgctgcaa ccttgcccat ctttataggt ctctttttca
     3541 agaaacgctt tgcctggtat gaagtgttgg taagtctctt ctttattgtc accatgttgg
     3601 tgggtggaaa gaccaatcaa ctagctgcct tgggtattta cctttgctgg gaaatattgc
     3661 tcctgctttt ctacaagcat tatcgaaaaa gcaaggatgg caagtgggtc ttctacttag
     3721 ttagttttct gtccctactt ccgattatct ttgtcaaggt gcaaccagct atcaatggaa
     3781 cgcagtcttt gcttgggttc ttgggaattt cttatcttac ctttcgttcg gttgggatta
     3841 tcatcgagct gagagatgga gtgattaagg attttaccct ctgggaattc ctccgtttcc
     3901 ttctcttcat gccaactttc tcgagtggtc caatcgatcg ctttaagcga tttaatgaaa
     3961 attatcaggc tattcctgag cgagatgagt tgatggatat gctggatgaa tctgtccgct
     4021 atatcatgtg gggctttttg tataagttta tcctagctca tgttttagga gagaccttac
     4081 tacctcctct gaagaattta gccttgcagt caggtggctt ctttaatctc tatgccttgg
     4141 cagttatgta tacttttggt ctggagctct tctttgactt tgcaggttat tctatgtttg
     4201 ctttggccat ctcaaacttg atgggaatcc gtagccctat caactttaac aagccctttt
     4261 tatcaaggga tttaaaggag ttttggaatc gctggcatat gagtctgtcc ttctggttcc
     4321 gtgactttgt ctttatgcga atggtgatgg tgttaaccag aaagaaagtc tttaaaaatc
     4381 gtaatgtaac ctcaagcatg gcctacattg taaatatgct gattatggga ttttggcatg
     4441 gtgtgacctg gtactatatc gcctatggac tctttcatgg actaggcttg gtcatcaatg
     4501 atgcctgggt tcgcaagaaa aaaacactca ataaggaacg gaaaaaagca gggaaggctg
     4561 ccctacctga gaatcgctgg attcagttgc ttggcatggt tgtcactttc catgttgtca
     4621 tgttgtcatt cttaatcttt tctggattct tgaataatct atggtttaaa aaataaaagg
     4681 aaataaaata tggatatcaa atcagaagtt atcgaaatta ttgatgagtt gtttatggaa
     4741 gatgtttctg acatgatgga tgaagatctt tttgatgcag gtgtcttgga tagtatggga
     4801 acggttgagt tgattgtgga gattgagaac cgttttgaca ttcgtgtccc tgtaacagag
     4861 tttggtcgtg acgactggaa tacagctaat aaaatcatag ctggtattgt ggagctacaa
     4921 aatgcttaaa cgcttatgga tgatcttcgg accggtcttg atcgctggtt tgttggtttt
     4981 tctgctcatt ttcttttatc ctactgagat gcatcataat ctaggagctg aaaagcgttc
     5041 agcagtggct actactatcg atagttttaa ggagcgaagt caaaaagtca gagcactatc
     5101 tgatccaaat gtgcgttttg ttcccttctt tggctctagt gaatggcttc gttttgacgg
     5161 tgctcatcct gcggtattag ctgagaaata caatcgttcc taccgtcctt atcttttagg
     5221 acagggggga gctgcatcgc ttaaccaata ttttggaatg caacagatgt taccacagct
     5281 ggagaataaa caagttgtgt atgttatctc acctcagtgg ttcagtaaaa atggctatga
     5341 tccagcagcc ttccagcagt attttaatgg agaccagttg actagttttc tgaaacatca
     5401 atctggggat caggctagtc aatatgcagc gactcgctta ctgcaacagt tcccaaacgt
     5461 agctatgaag gacctggttc agaagttggc aagtaaagaa gaattgtcga cagcagacaa
     5521 tgaaatgatt gaattattgg ctcgttttaa tgaacgccaa gcttcctttt ttggtcagtt
     5581 ttcggttaga ggctatgtta actacgataa gcatgtagct aagtatttaa aaatcttgcc
     5641 agaccagttt tcttatcagg caatagaaga tgttgtcaaa gcagatgctg aaaaaaatac
     5701 ttccaataat gagatgggaa tggaaaatta tttctataat gagcagatca agaaggattt
     5761 gaagaaatta aaggattctc agaaaagctt tacctatctc aagtcgccag agtataatga
     5821 cttgcagttg gttttaacac agttttctaa atctaaggta aacccgattt ttatcattcc
     5881 acctgttaat aaaaaatgga tggactatgc tggtctacga gaggatatgt accaacaaac
     5941 ggtgcagaag attcgctacc agttagaaag tcaaggtttt accaatatag cagatttttc
     6001 taaggacggc ggggagcctt tctttatgaa ggacaccatt caccttggtt ggttgggttg
     6061 gttggctttt gacaaggcag ttgatccttt cctatccaat cccacaccag ctccgactta
     6121 ccatctgaat gagcgctttt tcagcaaaga ttgggcgact tatgatggag atgtcaaaga
     6181 atttcaatag ataatataat atagaagagt tcaagaatga aactagtttt cacgtttttt
     6241 cttgactctt tttttgcttg tgatataatt aactggtaaa caaaatgtca aagaatagta
     6301 tttttctctt aaaaaagaga agtctaaaag gaaaggagtc agatatgaga cagctagcaa
     6361 aggatatcaa tgcttttttg aatgaggtga ttttgcaggc ggaaaatcag catgaaatcc
     6421 taataggtca ttgcactagc gaggtggccc tgaccaatac tcaggagcat atccttatgc
     6481 tcttgtcaga ggaatcttta acaaattcag aattggcccg tcgtctcaat gtcagtcagg
     6541 cggcagttac caaggccatt aagtctttgg tcaaggaagg gatgttggaa acatctaaag
     6601 attctaaaga tgcgcgtgtg attttttatc agttgactga cttggctcgt ccaatcgctg
     6661 aggagcacca ccatcaccat gagcatacac ttttaaccta tgaacaagtg gcgactcagt
     6721 ttactccaaa tgaacaaaaa gtgattcagc ggtttttgac tgctttagta ggagaaatca
     6781 aataatgaga tatattacgg tagaggattt gtccttctat tatgataagg aacctgttct
     6841 tgaacatatc aattattgtg ttgatagtgg ggaatttgtt accttgactg gggaaaatgg
     6901 agcggctaag acgacgctca tcaaggccag tcttggaatt ctgcaaccac gcattggaaa
     6961 ggtggctatt tcaaagacaa atacgcaagg taagaaattg agaatagcct atcttcctca
     7021 acaaattgcc agttttaatg ctggttttcc aagtacggtc tatgaatttg tcaagtcggg
     7081 tcgctatccg agaaaaggct ggttccgtcg tttgaatact catgatgagg agcatatcaa
     7141 ggctagtctg gactcagttg gcatgtggga acatcgagac aaacgcttgg ggtctctatc
     7201 tgggggacaa aagcagcgag cggtaattgc gcgtatgttt gcttctgacc ctgatgtgtt
     7261 tatcctagac gagccgacaa cggggatgga tgcaggaagt aaaaatgaat tttacgaact
     7321 catgcaccac agcgcccatc atcatggcaa ggctgttttg atgattaccc atgaccctga
     7381 agaagttaag gattatgcgg atcgcaatat tcatctagtc cgtaaccaag actcgccatg
     7441 gcgttgtttc aacgttcatg agaatgggca ggaggtgggc catgcttagt ttattatctt
     7501 acgactttat acaacgcgcc tttttggcgg ttattgctat gagtcttttc tcaccggtat
     7561 tgggaacctt cctcatcttg cgtcgtcaga gtttgatgag tgataccctt agccacgtct
     7621 cactttcagg tgtagccttt ggtctggttt tggggatttc tccaactgtt tctactattg
     7681 ccattgtctt gattgcggcg gtctttctgg agtatctccg tacggtttac aagagcttta
     7741 tggaaatcgg gacagctatc ctcatgtcaa caggtttggc tgtttctctg attgtcatga
     7801 gcaagggtag aagctcgagt tcaatgagtt tggaccaata tctctttggt tcgatcgtga
     7861 ctatcagtga agaacaggtc atttccctct ttgtcattgc ggcggttgtt ttgattttga
     7921 cctttctctt tcttcgtcct atgtatatct taacttttga cgaagatacg gcctttgtgg
     7981 atggcttgcc agttcgtacc atgtccattc tttttaacat ggtgacaggg gtggctattg
     8041 cccttatgat tcctgcagca ggagctcttc tggtatcgac cattatggtc ttgccagcta
     8101 gtattgccct gcgtctgggg aaaaacttta aatcggttat gctgcttgcc agtgcgattg
     8161 gctttttggg aatggtagca ggactttaca tttcctacta tgcagaaaca cctgcaagtg
     8221 caagtattac cattattttt gtaactgtct ttatactaat cagtttagta agacgtttta
     8281 tcaaatagga gacgaacgtg aaaaaaatta gcttattact agccagtcta tgtgccttgt
     8341 ttttagtggc ttgttccaat caaaaacagg cagatggtaa actcaatatc gtgacaacct
     8401 tttaccctgt ctatgaattt accaagcaag tcgcaggaga tacggctaat gtagaactcc
     8461 taatcggtgc tgggacagaa cctcatgaat acgaaccatc tgccaaggca gttgccaaaa
     8521 tccaagatgc agataccttc gtttatgaaa atgaaaacat ggaaacatgg gtacctaaat
     8581 tgctagatac cttggataag aaaaaagtga aaaccatcaa ggcgacaggc gatatgttgc
     8641 tcttgccagg tggcgaggaa gaagagggag accatgacca tggagaagaa ggtcatcacc
     8701 atgagtttga cccccatgtt tggttatcac cagttcgtgc cattaaacta gtagagcaca
     8761 tccgcgacag cttgtcagca gattatcctg ataaaaaagt gacctttgag aagaatgcag
     8821 ctgcctatat cgaaaaattg caagccttgg ataaggctta cgcagaaggc ttgtctcaag
     8881 caaaacaaaa gagctttgtg actcaacacg cagcctttaa ctatcttgcc ttggactatg
     8941 gactcaaaca agtcgcaatc tcaggacttt ctccagatgc agagccatca gctgctcgct
     9001 tggcagaatt gacagagtat gtcaagaaaa ataaaatcgc ctatatctat tttgaagaaa
     9061 atgcctcaca agcccttgct aacacacttt caaaagaagc aggtgtcaaa actgatgtcc
     9121 tcaatccttt agaaagtctg acagaagagg acaccaaggc tggagaaaac tacatttccg
     9181 tgatggagaa aaacctcaag gctttgaaac aaacaacaga ccaagaaggc ccagcaatcg
     9241 aacctgaaaa ggcagaggat accaagacag tccaaaatgg ttacttcgag gatgcagctg
     9301 tcaaggaccg caccttgagt gactatgcag gtaactggca atcagtttat cctttccttg
     9361 aagacggcac gtttgaccaa gtctttgact acaaggctaa gttgactggt aagatgaccc
     9421 aggctgagta caaggcttac tatacaaaag gctatcagac agatgtgact aagattaaca
     9481 ttactgataa tactatggaa tttgttcaag gtggacaaag caagaaatac acttacaagt
     9541 atgtcggtaa gaaaattttg acttacaaga aaggcaatcg tggcgtgcgt ttcctctttg
     9601 aagccacaga tgctgacgct ggacaattca agtatgttca gtttagtgat cacaatgttg
     9661 ccccagttaa ggcagaacat ttccatatct tctttggagg cacaagccaa gaagccctct
     9721 ttgaagaaat ggacaactgg ccaacctact acccagataa cctatctggc caagaaatcg
     9781 cccaagaaat gttggcgcat tgatgagaaa tcgactagtt cataagagct agtcggtttt
     9841 ttgatactag atggctaact tcattataga cctttcagaa ctttaaacaa taaataaaac
     9901 tcttgaaata tctgggctta tatgctagaa tgtagttagt gtataaggtt cattagaaca
     9961 ccaaagcccc taactagtgc catagttaag ggcttttttg acgtatctta gcgatttaac
    10021 gggtgtttat aggctagtca gtcggtctac ttcttaccat ctagccattt gcagacgaaa
    10081 tacaagataa ttccagctat aacatctgct ataatagtaa gagcaagctc tggtataagg
    10141 ctcattagtt tcacctcctc tcacgaaccc ataggaacgt aatcggtaac cgatgacaaa
    10201 aatagtatac cacactaaat ttagatcatc aacctcactt aatgcttgaa ctattgtaga
    10261 ttatatgcta aaatgtagtt aattgataag gttcattaga acaccaaagc ccctaactat
    10321 ggccgtagtt aagggctttt ttgacgtatc ttagcgattt aacgggtgtt tataggctag
    10381 tcactcggtc tactccttac tatctagcca tctgcagacg aaatacaaga taattccagc
    10441 tataacatcc gctataatag taagagcgag ctctatgata aggctcatta gtttcacctc
    10501 ctctcacgaa cccataggaa cgtaatcggt aaccgatgac aaaaatagta taccacaata
    10561 tatttagatc atcaaggtca cttaattctt gaaatatagg gtctaggcga caaagtcttt
    10621 aaaatcagaa aaacgcataa tatcaggtgt gcaaaaactt gatactatgc gttttattgt
    10681 gggaaggttt actccatttt ctcctgaaat tgagtttttg tccagcctct gtttttagaa
    10741 ttgctaagaa aataatgtca tgtggtgaat atttgtaaat cagtcagcag acagaacgaa
    10801 tactcttcga aaatctcttc acatcatgtc agcttcgtct tgccgtgtcc atgggggact
    10861 tgacttgatc agttttcaaa tctgtacttt gagcaagctg agactagctt cctatttgat
    10921 tttcattgaa tatcagaaac ccattctcca tcaaataatt cgactgcgtc taataatttt
    10981 tggtctggca aggtgtcaga aataaaggtt gtgtatttgg agaggggatt aattttaaaa
    11041 aatccagtct tgtaaaattt agaactatca atcagtaaga tggtttcatg ggctttgtca
    11101 ataatattct tttttgaaat agcttggctg agagaagctt cataaacata ttggtcatca
    11161 atacctcttg ctgaacaaaa tgctaaatcg atattaaaat gatctaataa agaattttcc
    11221 ttatcatagt tgaccacgga acaggattga tgtttgacct cgccagatgt gataaagatt
    11281 ttggagctat ctttaacagt ttcagatagg gtttgtgcag tatgtaaacc atttgtaaaa
    11341 ataatcaaat tatcaagttc agaaagatag ggacaaagtt cgtagacagt agtactagaa
    11401 tctagataga tacacatacc agaccgaata aagtctttag cgagactagc gattagtctt
    11461 ttttgcctag tactttctcc ttcccgtatt tgatgagaaa gttcaattgt gttcatagag
    11521 gacagggtca cgtatccgtg ctttcttttg ataagacctt gattttctaa gaaaattaaa
    11581 tcacgacgta aggtacttgt gctggagaaa gtgatttctg ccagctcttt tacggcaatt
    11641 ctttttttct ttttaataat ttcaatcaat tcaagtacac gttcatcttt tatcataagc
    11701 tcctcctaat ttatcatttc aactatatta tagcacaaat tggaggaatt tgaattattt
    11761 ttatgaatat tgggttaaca tttgaacatt attcaagtaa gcgttcacat attgaaaaaa
    11821 taaaacgtgg ggattataat aaagttaatc aaggacgaag agagaagaaa aatggaagcg
    11881 gttttagcaa tagatttagg tgcgacttct ggaagagcaa tcgttggtta cctttctgaa
    11941 aataaactag taatggaaga aataaatcgc ttttctaatc tacctattag agtaaaaggg
    12001 catttatctt gggatattga ctttctacta gctaaaattc ttgaaagtat ccgcttggct
    12061 aatactagtt acaagatttt atctatcggt attgacacat ggggagttga ttttggactg
    12121 attgataatg aaggtaagct gttatcacaa cctgttcatt atcgtgatga aagaacaaag
    12181 ggagtgttaa aggaaatatc tgaaatgact gaattagaaa aactgtattc agagacagga
    12241 aatcagatta tggagataaa taccttgttt caactcttta aggcacgtca agaatctcct
    12301 gactctttct ataagaccaa taagattctt ttaatgccag atttgtttaa ttatctcttg
    12361 acaggtaagt ttgctacaga aaaaagcatt gcttcaacaa ctcaattatt tgatcctagg
    12421 agtcaaaatt ggaatcagaa tatcttaaaa ctatttgaat tggattcatc tttacttcct
    12481 gaaattgttt cagagggaaa tgttcttgga aggataaaaa aggagtatgg tttaggcgat
    12541 attcctgttg tgaatgtttg tagtcatgat acagcaagtg cgattgtctc agtacctaag
    12601 acagaaggta gtttatttat ttcatcaggt acttggtctt tggttggagt ggaacttact
    12661 tcaccgattc ttactaccga atccttcagt tatggattta caaatgaagt cggtaaagat
    12721 ggagtgatta catttctgaa gaattgtaca gggttgtgga tcatagagga actaagacgt
    12781 tcatttgaac gaagagggaa agcctattct tttgatgata ttaggacaat ggtggagaaa
    12841 gaaaaagaaa atcttcctct gattgatact gaatcaactg aatttgcaac agaatctgat
    12901 atgcacaaga ctttgacaga atatctagct tatcatcatg aaactagaga gtggacagat
    12961 ggacaactat ttaagattgt ttatgaaagc ctagctgaaa cgtataggaa agcgatagag
    13021 ttattagaag aactaactca taaggtttat aagaggatat atgtgattgg aggaggtgct
    13081 agagccagtt attttaacca aatgattgct gatagaactg gtaaagaggt tcttacaggt
    13141 ttgactgagg ctacagctgt ggggaatatt gttgtgcagc tcatagctat gggacaatta
    13201 aaagggatgg aagaggctca ccatgttatt gaggagtttc tacaattaga gagttattac
    13261 tcccaaaaga attaaaaaga ttgagagttt gtaaatttgc ctccctcccc cttcttagct
    13321 tttgtgcagg aaggggggat aattggtgaa ttgaaaaata tttagtgttt tgatatgagg
    13381 aggacaagga tgtcagatgt aaaacaagaa ttaattaaat atggtaagaa gctagtagaa
    13441 acagatttga cgaaaggaac aggtgggaat ctcagcgttt tcgatcgtga aaaacaattg
    13501 atggcaatta ccccgtcggg tattgatttc tttgaaatca aagaatccga tattgtagtg
    13561 atggatatta atggaaatgt tgtagaggga gaacgcttgc catctagcga atggtatatg
    13621 catttgattc aatatcaaac tcgtgatgat atcgatgcaa ttatccatgc tcatacaact
    13681 tatgcaacag tattagcttg tctcagagaa ccacttccag cgagtcatta tatgattgca
    13741 gtggcaggga aagatgttcg ggtagctgag tatgcaacat atggcacgaa agaattggct
    13801 gtgaatgcag ctaaagcaat ggaaggtcgt agagcagttt tactagcgaa tcatggaatt
    13861 ttagcaggtg cacaaaattt attgaatgca tttaatattg ttgaagaagt tgaatattgt
    13921 gcaaaaattt attgtttagc taagaatttt ggagagccag tagttcttcc tgatgaggag
    13981 atggaattga tggcagaaaa atttaaaaca tacggtcaga gaaaataggg aggatattaa
    14041 tgttaaaaca tataccgaaa aatatttctc cagatttatt gaagacttta atggaaatgg
    14101 gacatggaga tgaaatagta ttagctgacg cgaattatcc ttctgcctca tgtgcaaata
    14161 agctaattcg ttgtgatggc gtaaatattc cagaattatt agattccatt ctgtatttaa
    14221 tgccattaga tagttacgtc gatagttcaa ttcagtttat gaacgttgtt tcgggtgatg
    14281 atattcctaa gatatggggt acctatagac agatgattga aggtcatggt acagatctta
    14341 aaacgattac ttatcttaga agagaagact tttatgaacg tagtaagaaa gcttatgcta
    14401 ttgttgctac aggagaaact tcactttatg ctaatattat ccttaagaaa ggagtagttg
    14461 ttgaaagaga aaatgtttaa tagaggaatt ttagttgcca gtcatggtga ttttgctagc
    14521 ggagctctca tgaccgcaga aatgtttgtt ggtgagacaa caaatgatag agttaggaca
    14581 ttaggtttga tgcctggaga gaatattgta gagtttgagc attattttaa aaatcaagtg
    14641 gatgaactgt tagactcaaa tcaagaggtt atcgttttga ctgacttgat tggaggaagt
    14701 cctaataatg tggctttgtc acggttttta aatttggatt cagttgatat tgtaacaggg
    14761 tttaatattc ctctcctagt ggaattaata tcaaattatg attcaaaaat caatttagaa
    14821 gaaattgttc acaatgctca aaatagtttg tttaatgtta aacaacaact taacgtagag
    14881 gaggaagaaa atttatgtct atagagtttg ttcgtattga tgaccgtctg gtacatggtc
    14941 aagttgtcac tacgtggcta aaaaagtatg atattgagca agttatcatt gttaatgatc
    15001 gcatctcaga agataaaaca cgacaatcta ttttaaagat ttctgcacca gtaggtttaa
    15061 aaattgtttt ctttagtgta aaacggtttg tggaagtttt aaactctgtg ccaataaaaa
    15121 agagaacaat gctgatatat acaaatccaa aagatgtgta tgattctatt gaaggaaatt
    15181 taaaattgga gtacctcaat gtaggacaga tgagtaaaac ggaggaaaat gaaaaggtaa
    15241 cgggaggtgt agctctaggt gaagaagaca aatattattt taagaaaata gttgataagg
    15301 gaacgagagt tgaaattcaa atggttccta atgataaagt tacaatgttg gaaaaatttt
    15361 tataaaaata atttaaggag gtacagtata tgctattcac acaagcatta ctggtgacat
    15421 tagttgggat tattgccact attgactata atggaccgtt atttatgatt caccgtccgt
    15481 tagttacaag tgcaatggtt ggcttagtct taggagattt cacccaaggt gttcttattg
    15541 gttcagctct tgaattaact tggctcggtg taacaggtat tggaggttac actccaccag
    15601 atactatttc aggtgcgatt attggtactg catttggtat tttatctggt caaggagaaa
    15661 ctgctggtat cgctatagca gttccaattg cagttgctac ccaacagttg gatgttcttg
    15721 caaaaacttt agatgtttat tttgtgaaaa aagctgataa tgatgctaaa aacggagatt
    15781 attcaaagat cggtttttat cattattcaa gtttggtttt aatcacgtta tttaaaattg
    15841 taccaatttt cctagctatt atgcttggag gggaatatgt ggcagacttg tttgctaagg
    15901 ttccaccaat cgttatgcag ggacttaact ctgcaggtgc tttactacct tcaattggtt
    15961 ttggtatgct tttaaatatg atgctcaaga aaaatatgtg ggtattcttg ttgattggat
    16021 tcatttgttc tgtgtatgga ggaatgtcaa ccattgggat ctcactagtt ggtattgcgg
    16081 tagcatactt ctacgatatg attggaagta aaccacaaga aacaacttca agtagtgatg
    16141 ttgaggagga tcttgatcta tgatgaataa taaagtaact aaagttgaac ttaaaaaagt
    16201 tttcaaacga agttttatgt atggttcttc atggaactat gagagaatgc agaacctagg
    16261 ttttctatat acaattcttc cagtattgaa aaaactatac ccagacaaag attcagcttc
    16321 tcctgcaatg aaacgtcacc ttgagttttt caatactcat caaacagcgg caccatttat
    16381 tcttggagtt acttccgcta tggaagaaca agaaggaaat gaaggtgcag cttcaattac
    16441 tggtattaaa gttggcttga tggggccact ggctggtcta ggagatagtt tgttctggct
    16501 gacactagtt cctatctgtt ttagtattgg tgcgtcttat tctaaagacg gcggtgcttt
    16561 aggtatcttt atcgccttaa tattgtttaa tattattaat attcctgtta aatatttcgg
    16621 tttgaaatat gggtatacta agggttctag tcttatccaa gaaaataata caaaaggaac
    16681 attgaatcgc gttacgagta tggcgacagc attagggcta gtactagtgg gtggtttgat
    16741 tccatcaatg gttggtatta attttggatt agaatttaag cagggggaac ttgttatttc
    16801 tgttcaagaa atgattacaa aattaattcc aggatttatc cctatggctt tgactttatt
    16861 aatgtgtaaa ttaattagaa aaggaaagaa tccggttgta ctaatcttta gtgttatggc
    16921 tattggagtt atcctagttg ttttaggaat tttgaagtag tagaaagtgt ggaggtggta
    16981 tttgggatat cacctccatt ttggaagaga ggtaaagagt gaaattatgg tataagaaag
    17041 ctgcctcaaa ttggaatgaa gccttgccga ttgggaacgg tcatttaggt ggtatgattt
    17101 atggttcagc tacaaaagaa tgtattcaac taaacgatga gactatttgg tatagaggaa
    17161 agtcagatag aaataatcca gactcactat tgcatcttaa aaaaattcgg gaatatcttt
    17221 tagatggaga aattcagaaa gccgaagaat tgataaagtt aacaatgttt gctaccccaa
    17281 gagatcaaag ccactatgaa ttacttgggg aactttacat tgagcatata gatattcagt
    17341 cttgtgctct ttcattgtat gaaagagagc tagatttaga tacagctatt tctaatgttg
    17401 tgtttgagcc taatagttgt aatttacaaa taaaaagaga atattttacg agttttaata
    17461 agaatatttt atgttgccgt atagtgtcat cagttcaaaa cacattaaat ttaaacatta
    17521 atttgggtag aaataaacgg tttaatgacg aagtatctaa actggattca agtacaattt
    17581 taatgtcggc ctctgctgga ggtagaaaag gtgttcagtt taaagtagta tgtcattcta
    17641 aagttacgga tggtgaagta agtgtattgg gagagacaat agttattcgg aatgctacag
    17701 aggtatttct ttatctcaaa tcaatgacgg attattgggg aaatatagat atttcttctc
    17761 ttcagggaga atttagtagt attgattact ttacagaaaa agatgaacat gtaaaaaaat
    17821 atcaggagca atttaataga gttgatttta aactagacta tagtaaagat tgtcttagca
    17881 ttccaacgaa tctacttctt gaaaacacta aaaagtatag taactacttg actaacttgt
    17941 tatttcatta tggaagatat ctgttaatat cgtctagtca accgaatggt ttacctgtca
    18001 atcttcaagg aatatggtgt gatgaattaa atccaatttg gggttctaaa tatacgatta
    18061 atattaatac tcaaatgaat tattggatgg taggtccatg tgatttacca gaagtagaat
    18121 atccattatt tgatatgctc gaaagaatga gagaaccggg aagactaacc gctaagaaaa
    18181 tgtatggagc tagaggtttt acagcacatc ataatacgga tggttttggc gatacggctc
    18241 cccaatctca tgccatgggg gctgcaattt gggtattaac tattccatgg ttatgtactc
    18301 atatttggga acactattta tatttccaag atgagcgtat tcttacggaa cattttgaaa
    18361 tgataaaaga agcatttctt ttctttgaag attatttatt tgaggtggat ggctacttga
    18421 tgacaggtcc aagtgtctca ccggaaaata aatatcgctt aaaaaatggt attgaaggaa
    18481 atgcttgtct atcatctaca attgataatc aaattctaag atatttttgt gattcatgca
    18541 ttggcattgc aaaacaatta ggagacaatt cggattttat tagtcgtgtg aaggagttaa
    18601 aaaagaaact acctaaaaca aaaataggta gtaatgggca aatccaagaa tggttagaag
    18661 attatgaaga agtagagcct gggcatagac acatttcacc tctatttggg ctttatcctt
    18721 ataatgagat tgatattcat aaaactccgg aattagcaga agcagctaaa atcactatca
    18781 ataggagatt atcaaacgct aactttttat cttcacagga gagggagcaa gcgattaata
    18841 attggttagt aagtggtttg catgctagta cacaaacagg ttggagtgct gcatggctga
    18901 ttcatttttt tgcgagacta tatcaaggtg aacctgctta taaccagatt aatggtttgt
    18961 taaataatgc gactcttggc aatttatttc ttgaccatcc accatttcaa attgatggta
    19021 atttaggttt ggtgagtgga atttgtgaat tattagtaca gagccatcat aattggttat
    19081 cactaattcc agctttacct tctgcttggt cagaaggaga agtgaaaggt ttcagagtaa
    19141 gaggaggata taaggtatcg tttgcttgga aaaatgggga tataacattc ctaaaattgg
    19201 aaggaggaaa caaagatcaa aaagtaagag taagaatata tggcaaaaat actgatgtac
    19261 aaaatattga attggtattt aattcagaaa aaattattga gttaaatttt taggtataag
    19321 tcatgaataa agaaaaaata aaaagaaaat taatcacaat actgtttgta tgtattggga
    19381 tgttatgttt tggattgtta gcaggagtta aggctgataa tcgtgttcaa atgagaacga
    19441 cgattaataa tgaatcgcca ttgttgcttt ctccgttgta tggcaatgat aatggtaacg
    19501 gattatggtg ggggaacaca ttgaagggag catgggaagc tattcctgaa gatgtaaaac
    19561 catatgcagc gattgaactt catcctgcaa aagtctgtaa accaacaagt tgtattccac
    19621 gagatacgaa agaattgaga gaatggtatg tcaagatgtt ggaggaagct caaagtctaa
    19681 acattccagt tttcttggtt attatgtcgg ctggagagcg taatacagtt cctccagagt
    19741 ggttagatga acaattccaa aagtatagtg tgttaaaagg tgttttaaat attgagaatt
    19801 attggattta caataaccag ttagctccgc atagtgctaa atatttggaa gtttgtgcca
    19861 aatatggagc gcattttatc tggcatgatc atgaaaaatg gttctgggaa actattatga
    19921 atgatccgac attctttgaa gcgagtcaaa aatatcataa aaatttggtg ttggcaacta
    19981 aaaatacgcc aataagagat gatgcgggta cagattctat cgttagtgga ttttggttga
    20041 gtggcttatg tgataactgg ggctcatcaa cagatacatg gaaatggtgg gaaaaacatt
    20101 atacaaacac atttgaaact ggaagagcta gggatatgag atcctatgca tcggaaccag
    20161 aatcaatgat tgctatggaa atgatgaatg tatatactgg gggaggcaca gtttataatt
    20221 ttgaatgtgc cgcgtataca tttatgacaa atgatgtacc aactccagca tttactaaag
    20281 gtattattcc tttctttaga catgctatac aaaatccagc tccaagtaag gaagaagttg
    20341 taaatagaac aaaagctgta ttttggaatg gagaaggtag gattagttca ttaaacggat
    20401 tttatcaagg actttattcg aatgatgaaa caatgccttt atataataat gggagatatc
    20461 atattcttcc tgtaatacat gagaaaattg ataaggaaaa gatttcatct atattcccta
    20521 atgcaaaaat tttgactaaa aatagtgagg aattgtctag taaagtcaac tatttaaact
    20581 cgctttatcc aaaactttat gaaggagatg ggtatgctca gcgtgtaggt aattcctggt
    20641 atatttataa tagtaatgct aatatcaata aaaatcagca agtaatgttg cctatgtata
    20701 ctaataatac aaagtcgtta tcgttagatt tgacgccaca tacttacgct gttgttaaag
    20761 aaaatccaaa taatttacat
//
LOCUS       DAVBCB010000043        20601 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVBCB010000043 DAVBCB010000000
VERSION     DAVBCB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20601
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.043"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..299)
                     /locus_tag="VQU28_001487"
     CDS             complement(<1..299)
                     /locus_tag="VQU28_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2476825.1"
                     /translation="MMYLDGGGVGCNWWNKRDNIAAAAVSGGMSVYQTYAIKKLIANR
                     GMMSRVVRSAITRYIGGVSMATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDG"
     gene            complement(279..347)
                     /locus_tag="VQU28_001488"
                     /pseudo
     CDS             complement(279..347)
                     /locus_tag="VQU28_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
     gene            complement(762..974)
                     /locus_tag="VQU28_001489"
     CDS             complement(762..974)
                     /locus_tag="VQU28_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000989850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476826.1"
                     /translation="MMVVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQIK"
     gene            complement(993..1352)
                     /locus_tag="VQU28_001490"
     CDS             complement(993..1352)
                     /locus_tag="VQU28_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476827.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WILPILLMAGMGDVIIN"
     gene            complement(1330..1668)
                     /locus_tag="VQU28_001491"
     CDS             complement(1330..1668)
                     /locus_tag="VQU28_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HET2476828.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVSGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            2134..2997
                     /locus_tag="VQU28_001492"
     CDS             2134..2997
                     /locus_tag="VQU28_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476829.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYLRQHGAGELSKHAEQVVKKLWKKIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRMTILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFSLLELTEETSMLSHLKK
                     EVEIYYQAKER"
     gene            complement(3049..3621)
                     /locus_tag="VQU28_001493"
     CDS             complement(3049..3621)
                     /locus_tag="VQU28_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HET2476830.1"
                     /translation="MKSITKKIKATLAGVAALFAVFSPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNGDYSAE
                     NQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            3863..4534
                     /gene="sdaAB"
                     /locus_tag="VQU28_001494"
     CDS             3863..4534
                     /gene="sdaAB"
                     /locus_tag="VQU28_001494"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HET2476831.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            4543..5415
                     /gene="sdaAA"
                     /locus_tag="VQU28_001495"
     CDS             4543..5415
                     /gene="sdaAA"
                     /locus_tag="VQU28_001495"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HET2476832.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            5437..6069
                     /locus_tag="VQU28_001496"
     CDS             5437..6069
                     /locus_tag="VQU28_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HET2476833.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            6095..6193
                     /locus_tag="VQU28_001497"
                     /pseudo
     CDS             6095..6193
                     /locus_tag="VQU28_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(6417..8267)
                     /locus_tag="VQU28_001498"
     CDS             complement(6417..8267)
                     /locus_tag="VQU28_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HET2476834.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMVDFWGLSASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVARAVDEAKVPKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(8335..9474)
                     /locus_tag="VQU28_001499"
     CDS             complement(8335..9474)
                     /locus_tag="VQU28_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HET2476835.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKGKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDAEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGGLILKIDAHSKVTESFVMNNVAVIQQGEFVCGGPRP
                     TIVKGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLSLLTLLTLLKHK
                     NGFLIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKGTVIYLEK"
     gene            9532..10698
                     /locus_tag="VQU28_001500"
     CDS             9532..10698
                     /locus_tag="VQU28_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HET2476836.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTRLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TAMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIDILMTVALFTVDRADKIL"
     gene            10837..11163
                     /locus_tag="VQU28_001501"
     CDS             10837..11163
                     /locus_tag="VQU28_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HET2476837.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            11150..11743
                     /locus_tag="VQU28_001502"
     CDS             11150..11743
                     /locus_tag="VQU28_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HET2476838.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEVAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            11736..12647
                     /locus_tag="VQU28_001503"
     CDS             11736..12647
                     /locus_tag="VQU28_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HET2476839.1"
                     /translation="MRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            12710..13774
                     /locus_tag="VQU28_001504"
     CDS             12710..13774
                     /locus_tag="VQU28_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476840.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIETPEGGEARTEIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(13846..14706)
                     /locus_tag="VQU28_001505"
     CDS             complement(13846..14706)
                     /locus_tag="VQU28_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HET2476841.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHIYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTETDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYEAIARLDYAKFLEVLPPSFYHQLDANQIEVQPILD
                     KDFKTLAQEK"
     gene            complement(14828..15814)
                     /locus_tag="VQU28_001506"
     CDS             complement(14828..15814)
                     /locus_tag="VQU28_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HET2476842.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEENEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(16086..17561)
                     /locus_tag="VQU28_001507"
     CDS             complement(16086..17561)
                     /locus_tag="VQU28_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HET2476843.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(17665..18588)
                     /locus_tag="VQU28_001508"
     CDS             complement(17665..18588)
                     /locus_tag="VQU28_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2476844.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(18602..19531)
                     /locus_tag="VQU28_001509"
     CDS             complement(18602..19531)
                     /locus_tag="VQU28_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2476845.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(19850..20200)
                     /locus_tag="VQU28_001510"
     CDS             complement(19850..20200)
                     /locus_tag="VQU28_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476846.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETADKDVLENSQEELQKAVLFLADEKGSEHTEAELI
                     DNLKEVIAKLKANA"
BASE COUNT         6263 a         4397 c         3708 g         6233 t
ORIGIN      
        1 ccatcaaatt taccgtctac gatatctaaa ccatatgcaa tggctcctcc taatgagaaa
       61 acttcagcaa cagtagccgc aatattaaga gcattagtaa tagttgccat actaactcca
      121 ccaatatatc ttgttatagc acttctaacg actcggctca tcataccacg attagcaatt
      181 aactttttaa tggcatatgt ctggtatact gacatccctc cagaaactgc agctgcagca
      241 atattatcac gtttgttcca ccagttacaa ccaacaccac ccccatcaag atacatcatc
      301 tcttcttgct caagaacaac ataattattt ggtaatacga gttccattta ttttctcctt
      361 ttttattaag catagttagg atcgtttcct aacaagtgat cgttgacttt gtttatagca
      421 cttagttacc tagataagat atcctttagc ttgcttccaa aatttaagtc tgcacctata
      481 atatgagttc atattagtac tgctaaatcc tatctatagt aatgtcaaac acctaaataa
      541 agtcatctct ccaattcatt caagaaatga agaaaggcaa aaaatcttta aaataagaaa
      601 agtgcatatt atcaggtgtt caaaaatctt gataatatgc gttatataat agaaaaattt
      661 actcaatttc actttcaaat tgagtttttt cccaacctcg tttttaattt atatcttcaa
      721 taaaatctaa tgacctagtt gatatgtcat atggaataat attatttaat ctgtaatttt
      781 tttaaaaaac ttaataataa atttgctcca ctgattgcta tcaatatttc aattggtaaa
      841 aaatagtaat tccaaataaa atgagaaatc agcaatgata gaaaaataac aatatcaatt
      901 ttcctgttgt tgacatactc tgtaatttta tggtacagga atgcaacaat aactgaaatt
      961 aaaacaacca tcataatact attatcctat tctcaattga ttatcacgtc gcccatccca
     1021 gccatcaata atattggtaa aatccaatca gctccagcac caattccagc agatgcaata
     1081 cttctaataa cagtatttgc aaaagcattt ccaatatgaa ttcctaatac tcctaaacct
     1141 ttaacaatcc cagctaccag tgcggagcgt aatccataag atgcaagaac tagttgaagg
     1201 cctccacctc ctgctgctgc taatgctgca tatgctgcac cagtaatggc tcctgtaatt
     1261 gcccacctgg gtatatatat agccccccca tcaaggtaca tcatctcttc ttcatcaatc
     1321 acaacataat tatttggtaa tacgagttcc atttattttc tcctttatac tatgtaatac
     1381 cacaaaatga ttttgaccta aatcattctt aacttgaaga atcgcaggaa atgttaattt
     1441 tggagtcaaa tcttccagtg ctactcgaac agcttttact gtaaaattca acttattcag
     1501 acctgaaaca ataccactga cagtcgttcc atacatgtct gtaccaataa tttcccgaat
     1561 cttcatgatt gatagttcct ttttgtaaga taaaagaact gttgaaacaa ctgccgcagc
     1621 acaatctgac tgatcatgtt gcatcgtaca aggatatttt tttaacatag aaatactcaa
     1681 ttctttagaa ttaatctaac ttttctttga tgtcacccaa acctcaatca ctgcccagta
     1741 gttcttgaat cagtttattt tcaatcgtat cattctttgt tcttgatatc tttccaagca
     1801 ttgtagtaca gcagattttt gtccaaggac taagcctcct ttaacaactc aagctccctt
     1861 acattatatc gaccagcccc atggaaagat tatggccaga ctaatcaatg atataataca
     1921 tactagaata caaaatattt ttttattcat actttttctc ctatctttat tttctaccta
     1981 catcatacct cttattaagc tcattttaaa attacgttaa cttttttcac aaatttattt
     2041 cgactttctt ttttactttt tacattgcta tgatacaata tttttaacaa aattttattg
     2101 tttgttaact tttttcacag aaaggagaaa atcatgcgtt atgatttcgg aaaggtctat
     2161 aaagaaatac gtgagtcaaa ggggctgacc caagaagagg tctgtggggg tgttctctca
     2221 agaaccagct tatcaaaaat cgaaagtggc aagacaaccc caaaatatga aaatatggaa
     2281 tttcttctcc gtcaaatcaa tatgagtttt gaagagtttg aatatatctg tcaactttat
     2341 caaccaagtc aacgcacaga gattatgcaa acctatctca atatgcgttc cattatcggg
     2401 accagcgatc ttgtcaattt atttcaaaaa tgccaagact acctcaagac ccaccacgat
     2461 ctccctatag aagaaatcag agatatgctt gaagttgtta tttatctccg tcaacacggg
     2521 gcaggggaac tatcaaaaca cgcagaacag gttgtcaaaa aactttggaa aaaaattgaa
     2581 aaacaagata catggtatga gagtgatcta aaaatcctca ataccattct tttcagtttt
     2641 cccattgaat acctccatct catcactgga aaaatattgc aacgcctaga agtctataaa
     2701 aactatcaac atttatatga cttgcgaatg acaattctat taaacctttc cacactctat
     2761 ctatacaatc aagataaaaa catgtgtaag caaatctgct acactttact ggaggacgcc
     2821 aagaataaga aaagctacga taggctggct atctgctatg tccgtattgg gatttgtaca
     2881 gacgattcta aacttatcca aaaagggttc tcccttctgg agctaaccga ggaaacttct
     2941 atgctgtctc atctcaaaaa agaagtagag atctattacc aagcgaagga aagataaaaa
     3001 agccgaggga tttcctcgac tctttacata ttatttgttc gttagttctt aataccagcc
     3061 attgttaagc cagaagtttt tagcagcagt ccatgaaccg taacgtcctg caacgtaggc
     3121 atctgctaca cgttcttggt tttcagctga gtagtcaccg ttcaggtatg aatctgttaa
     3181 ttggtaacgt ccgatataac gtccatttgt agctgtatag ctaccacctg attctttttg
     3241 agcgatccat tctttggctt ctgcttcaga tccgcttaca gttgaaacaa ctgtttcact
     3301 tactactgga gctgaaacag tttcatcttg agcggctggt gccgcataag tagctggcgc
     3361 tggtgttgca acaggcgcta cagggccatc gataaccaac tcttgatcaa cataaatcaa
     3421 atgaatgtta tcaatgtggt tgttttctgc caatttttca actgttgtgt tgtgagtttc
     3481 agcgatttct gaaagtgtat caccttcttt aacagtgtaa gttgatgatt cttgagcaga
     3541 tacaaatgat ggagaaaata ctgcaaacaa ggcagctact cctgcaagag ttgctttaat
     3601 ctttttagtt attgatttca tattcgaaaa ttctccttct ttctatagtt ctatcatacc
     3661 ctttaaatat tactgtttct tgacactttt atgtagaaat attacaaaat tattttttat
     3721 ttccctaaat aaggtgtttt ttgtcataaa agatactatt tttatgctat ttgaggtatt
     3781 agaaaggttt tcatccacaa ttaaagccaa gcccctcatt ctttgatata atagagataa
     3841 gaatttttat aaggggaaac tgatgaaatc acttcgtttt caatctgtct ttgatatcat
     3901 cggaccagtt atgattggcc catctagtag ccataccgct ggtgctgttc gtattgggaa
     3961 gattgtctct tccatttttg atgatactcc gacagaagtt gaattccaac tttttaactc
     4021 atttgccaag acctatcgtg gtcacggaac agacctagcc cttgttgcag gtattttagg
     4081 aatggataca gatgatcctg aaattccaaa tagtctggaa attgcccaca agcgtggtat
     4141 caagattgtc tggaccattc agaaagacag caatgcccct caccctaaca ccactaaaat
     4201 tactgttaaa aatgcccaca agaccatcag cgtgactggt atttctatcg gtggaggaaa
     4261 tattcaggta accgaactca atggttttgc cgtctctctc aatatgaata caccgactat
     4321 catcatcgtt catcaagata ttccaggtat gattgccctt gttacagagg cgctttcccg
     4381 ctatggtatc aatatcgccc agatgaatgt cactcgtgaa aaagctggtg aaaaagccat
     4441 tatgattatc gaagttgaca gtcgcaactg tgatgaggct atcgaagaaa ttcgaaaaat
     4501 ccctcatctc cacaatgtca atttctttaa ataggaggaa gcatgtttta ttctatcaaa
     4561 gaattggtcg agcaagcaga tctggacttt caaggaaatg tcgcagaact catgattaca
     4621 acagagtttg aattgaccgg tcgcgaacgt gaagaagtct tccttctcat ggaacgcaat
     4681 ctggaagtta tgaaagcctc tgtccaactt ggcctcaatg aaaataaatc tcgtagtggc
     4741 ctgacaggtg gagatgctgc caaattggat cactacatca aaaacgaaaa aactttatca
     4801 gattacacga ttctctctgc tgcccgaaat gctatcgcag tcaatgaaca caatgctaaa
     4861 atgggcttgg tctgcgctac tcctaccgca ggaagtgccg gttgtctccc ttccgttctc
     4921 actgctgcta ttgaaaaatt agacctcagc cacgagcaac aattggattt cctctttgct
     4981 gctggtgcct ttggactagt catcgcaaac aatgcctcca tctcaggtgc tgagggtggg
     5041 tgtcaagctg aagttggttc agcctctgct atgagtgctg ccgccttgac tctggctgca
     5101 ggtggaacac cttatcaggc cagtcaagct attgcctttg tcattaaaaa tatgctaggc
     5161 ctcatctgtg accctgttgc aggtttggtc gaagttccct gtgtcaaacg taatgccatg
     5221 ggagctagct ttgctttcat cgcagcagac atggccttgg caggtatcga atctaaaatc
     5281 cctgtggatg aagtgatcga tgccatgtac caagtaggag caagcatgcc aactgccttt
     5341 cgtgaaacag ctgaaggtgg actcgctacc acccctactg gtcgtcgcct ccaaaaagag
     5401 attttcggag aataagctta tcaaatagga gaaaccatga cctctatcac agcgattttt
     5461 ttcgatttgg atggaaccct cgttgatagt tctatcggga ttcacaatgc ctttacctat
     5521 acctttaaag agctgggggt gcctagccct gatgccaaaa ctattcgtgg ttttatggga
     5581 ccacctctcg aaagtagttt tgcgacctgc ctgtccaaag accaaatttc tgaagccatg
     5641 cagatatatc gttcttacta taaggcaaaa ggcatctatg aagctcaact ctttcctcag
     5701 attatagact tgcttgagga gttatcgagc agttatccac tctacatcac cacgacaaag
     5761 aatacatcta ctgctcaaga catggccaaa aacttggaaa tccatcattt ctttgatggt
     5821 atttatggtt ctagccctga agcaccccat aaggcagatg tcattcacca agccttacag
     5881 acacatcaac tagcaccaga acaagccatc atcatcggag ataccaagtt tgatatgctg
     5941 ggagctcgag aaacaggtat tcagaaattg gccgtcactt ggggatttgg agagcaagca
     6001 gatctactaa actatcaacc tgattatatc gctcacaaac ccttagaagt tttggcgtat
     6061 tttcaatagc atagactggg cgacaactct atttcagaag aaaatgaagt aatactcaat
     6121 gaaaatcaaa gagcaaacta ggaaactagc tgcgggctgc tcaaaacact gttttaaggt
     6181 tgtagatgga actgacgaag tcagctcaaa acactgtttt gaggttgtgg atagaactga
     6241 cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag tcagtaacca
     6301 tatacggcaa ggcgacgttg acgtgatttg aagagatttt cgaagagtat aagtctctac
     6361 ataataaaac gcatattacc aaggttattt aacttgataa tatgcgtttt tcttttttat
     6421 tcaatgtggg ttgtttgatt agcaaaggcg ataatagctt gcttcaactc atctccactg
     6481 agagtgcgga actctccaat cttttgattg atggattcta aaggcatgac attaacctta
     6541 ccaacgaaaa tcttatccat aacttgatta tcaacgagtt cggttgatac caagagttct
     6601 tcgtagagtt tttcacctgg gcggattcca acttcaacga ttggaatttc actttcagtg
     6661 tggccactta gaagcaccat cttcttggcc aagtcataaa tcttgactgg tttgcccata
     6721 tcaaggataa agacttcccc atctttggca taagcaccag catggataac cagacggcta
     6781 gcttctggaa tggtcataaa gtaacgggtc atgcggaagt ctgtcaccgt tacaggccca
     6841 ccttcagcaa tctgacgttc aaagactgga atgacactac cacggctacc aagaacattc
     6901 ccaaaacgaa ctgcacagta ggttgattgg ctacgttggt taaagccagt gacaatcaac
     6961 tccgccacgc gcttggttgc tcccataaca tttggtggat tgactgcctt atctgtcgaa
     7021 atcataacca tcttaggcac cttggcttca tcaacggctc tagcaacatt gtaagttcca
     7081 cggatattgt ttttgaaggc ttcttttgga ttgcgctcca tcataggaac atgcttgtgg
     7141 gctgccgcat gataaacaat agcaggtttg tactgctcaa agacttgcaa caaacgatca
     7201 tagtcttgaa tgtccgcaat cacaggtaca taatcaatcc cttggaactt acgaatcaat
     7261 tcatgataaa caaggtagat tgagttttcc ccatgaccga gcaagacaat gcgttcagga
     7321 ttgaagcgac taacttgacg acagatttca gaaccgattg aacctccagc tcctgtgact
     7381 aagatggtct tacctgtcag ttctgcaccc agacgcgatt cgtcaagacg gatttcctga
     7441 cgacccaaaa ggtccgtaat atcaattttt tggaagccag tacctgcttg gtgaaggccc
     7501 tgaacaacag tttcaacctt aggcatcttg taacatttga cacccagctt attacacatc
     7561 tgcaagatac gctcatattc tgacggatcc agcgacggaa tcgcaacgat gacacgctcg
     7621 atttgatggc gtttggctaa ttcaggcaga ttgtcataag agcccaaaac aggaatacca
     7681 ccaagttttt gacccttttt cttagaatcc ttatccaaaa taccgaccag ttctaattca
     7741 ctggttggat gttggtaact atccataaag agagccccac catcaccggc accaatcaag
     7801 aaggtccgac ggtgttctcc atcaccacta ccttttttgc gtctggagta gattaactgc
     7861 caagtaatcc gtggcaataa aatcaagaag gtactcaaca agataaagag aatgataaaa
     7921 cggatggaga agagtggcaa gaaggcataa cagatactat atgacaagac actgctagca
     7981 gtcacaccaa aaaagatttt catgaaatcc gtaatcttgc tgtaacgact aatactcgcg
     8041 ctcaagcccc aaaaatcaac catcaattga tagaacagga aggccaaact cgtatagata
     8101 atgtagtcaa caggtgctgg attaatcagc ccataaaata aaatataaga tacaatgatg
     8161 gaaaccacca tactgaaaat atcaaatatt ccccagaaaa cctgcttttg ttgtttattt
     8221 aaaatttcca ccagatcaat cacataatct gtgagttttt tattcataga aatttactcc
     8281 tccttaaaat agttctacta tattgttatt atagcacatt ttaatttatt tggcttattt
     8341 ttccaaataa attactgttc ccttgtactc cttcttccat ttaaatcctc taattaaacc
     8401 tacaatcgtc ccaaggccat aagcaaagtg aatggaaaat aaaagaaagg gcatcacaat
     8461 tagaaatcca tttttatgtt ttaataaagt cagcaaagtg agtaatgaca gtaatgataa
     8521 aagtagaaaa taggcaccta atagtaaagt tatgaatacg aatgtgatcg gtaacaatgc
     8581 tagactaaac acaagactca aaacaaataa acaaggaaca taatgaaata atgataaaca
     8641 cttaaactga acatgacttg tcaagccaat ccacaaacca tttgaatact tttgatgcag
     8701 cattttcttg aatgttggtc gaatatactg ataagataga atacttgggc tatagcggat
     8761 tttataacca tgttctcgaa ttctataatg aatatcatta tcttcagttc ggccaagttg
     8821 ctcatttact aaaccaacct tctggaaaac ctctcgttta tacatcccat gaaaaataga
     8881 agaaacatat ctatcctcgg aactatttcg ataattggca atgctactgc caaacatatt
     8941 ttcctcaaca agatgcaagg tctctgccca ttttcctttt cctttgacaa tcgtcggtct
     9001 aggccctcca cagacaaatt caccttgttg aataacagcc acattgttca ttacaaaact
     9061 ctcagtaact tttgaatgag catcaatttt taaaataagg ccccctacag aatgcttaac
     9121 tcccaggtta aaaccactag cttgattttt cttaggattg ttatacaatc taattgagtt
     9181 aaactctgca tcttctttta taaattgctg aatgatagct gtggtcccat ctgtggacat
     9241 agcatttata aatagaattt caatattctc tttaggatag gtttgatttt ttaagtcttc
     9301 aattagacca ggcagatatt tttcttcatt gtatgcactg attacaatgg atacgagaag
     9361 actttcaaca ttttgagtca ctactactct cctgaatttt cccttccacc attctatcat
     9421 aattttcaag acttttccat ttttatcttg aaagccagaa accttacttg acattatagt
     9481 gaaaaagcct taaaataagc tgttattaaa atcagctaga agagatatta tatgaaaaag
     9541 caatcactct tttttgttcc aggtattatc ctgattggtg tttccttgcg aactcctttt
     9601 actgttttac ccattatttt gggaaatatt tcgcaaggtc tggaggtaga agttagttcg
     9661 cttggtgtct tgaccagcct gcctctcctt atgtttaccc tcttttcacc attttctacc
     9721 cgactggctc agaaaatcgg cttggagcat ctcttcacct acagcctctt cttcttgacc
     9781 atcggctcac ttattcgact aatcaatctg cccctgctct atctaggaac cttgatggtt
     9841 ggggcaagtg tcgcagtcat caatgtcctg cttcctagtc ttatccaagc caatcaacca
     9901 aagaaaattg gttttctgac caccttatat gtaacgtcta tggggattgc aacggctctg
     9961 gcttcctatc tagctgtgcc cattacacaa gccagttctt ggaaaggact tatcctcctc
    10021 ctcacgttac tctgtctagc aacttttttg gtctggctcc caaatcaccg ctataatcat
    10081 agactagctc cacaaaccaa acaaaaaagt caaataaagg tcatgcgtaa taaacaggtt
    10141 tgggcaatta ttatcttttc aggttttcaa tccttgatct tttacaccgc catgacctgg
    10201 ttacctacca tgtctatcca tgcaggtcta tccagtcacg aagctggctt gctgacttct
    10261 atcttatctc tgattagcat tcctttttca atgaccatcc caagcctgac aaccagttta
    10321 tctactcgca accgtcagct catgctcact ctggtttcac tagctggtgt ggtcggcatt
    10381 tccatgctct ttttcccaat caataatttc atttactggc ttgccatcca tctcctcatc
    10441 ggaaccgcaa ccagcgccct cttcccttat ctcatggtca acttttcact caagaccagc
    10501 gcccctgaaa agacagccca attgtccggc ctatctcaaa caggaggcta tatcctagca
    10561 gcctttgggc caaccctctt tggttacagt tttgacctat tccactcttg ggtaccagct
    10621 gtagctgccc tcttgctcat cgatatcctg atgactgtgg ccctctttac agtggacaga
    10681 gcggataaga tcctttaaac ttctagtttt agctagaagt tttttatata taaaaatttt
    10741 acacatttct cttgacaggg ctttctttta gatgtacaat gtatgtgtag aaaaattata
    10801 tataaaaatc ttacacatta gaaaaggagg tttcccatgt actttccaac atcctctgcc
    10861 ttgattgaat ttctcatctt ggctgtactg gagcagggtg attcttatgg ttatgagatt
    10921 agccaaacca ttaagctgat cgctaatatc aaagaatcca cactctatcc cattctcaaa
    10981 aaattggaag gcaatagctt tctgacaacc tattctagag agttccaagg tcgcatgcgc
    11041 aaatactact ccttgacaaa cggtggtata gagcagctct tgaccctaaa agatgaatgg
    11101 acactctata cagacaccat caatggcatc atagaaggga gtatccgcca tgacaagaac
    11161 tgaatacctg actcagctag aactctatct caagaaacta cctgaagctg accgtatcga
    11221 agccatggac tatttcagag agctctttga cgatgctgga gtcgaaggag aagaagaact
    11281 catcgctagt ttgggaactc ccaaagaagt ggcccacgaa gttctatcca atcttctcga
    11341 taaaaaaatc aatgaagcac ccgctcaaaa aaataaccga caaattttac atatcgcctt
    11401 gttagccctc cttgcagcac ctatcggcat tcctctggga atcgccatcc tcgtgaccct
    11461 gttcgcaatc cttgtagccg ctttgactgt cattctggct ttctttgcag tttccatact
    11521 gggtatcatc ggcggattcc tatttttagt tgaaagtttc actgtcctcg cccaagccaa
    11581 atcagccttt atcttgattt ttggttctgg tttactggct atcggtgctt cttcgctagt
    11641 tttacttggc atttcctatg tagctcgctt cttcggtcta ctcattgttc gtctggtaca
    11701 atttgttctt aaaaaaggaa agagaggtaa tcagcatgcg taaatggaca aaaggatttc
    11761 tcatctttgg tgtggtgact accgttatcg gctttatcct gctttttgta ggtatccaat
    11821 ctgacgggat taagagccta ctttccatgt ccaaagaacc tgtctatgat agccgtacgg
    11881 aaaagctaac ctttggcaag gaagtcgaaa acctagaaat tactctccac caacacacgc
    11941 tcaccatcac agactctttc gatgatcaaa tccacatttc ttaccatcca tctctttctg
    12001 ctcaccatga tcttatcacc aatcagaacg atagaactct gagtctcact gataagaaac
    12061 tgtctgaaac tccgtttctc tcttctggaa ttggtgggat tcttcatatc gcaagtagct
    12121 actctagtcg ttttgaagaa gttattctcc gactaccaaa agggagaact ctaaaaggga
    12181 tcaacatctc agccaatcgc ggacaaacca ccatcataaa tgctagcctt gaaaatgcga
    12241 ccctcaatac aaacagctat atcctccgaa ttgaaggaag tcgtatcaaa aacagtaaac
    12301 tcacaacgcc caatatcgtt aatatctttg atacagttct tacagatagt cagctagagt
    12361 caacagagaa tcacttccac gctgaaaata tccaagtcca tggtaaggtt gaactgactg
    12421 ccaaagatta tctcagaatc atcctagacc agaaagaaag ccaacgaatt aactgggaca
    12481 tctcaagcaa ctatggttct atcttccaat tcacaagaga aaagcctgaa tcaagaggta
    12541 cggaattaag caacccttac aaaactgaaa aaaccgatgt caaggatcaa ctcattgcga
    12601 gatctgatga taatattgat ctaatatcca caccaagcag acgttgacaa aaacgcttac
    12661 atctgctact atggaagcat atttatctaa ctaaaaagga ggttctacca tgaaacaaga
    12721 atggtttgaa agtaatgatt ttgtaaaaac aacaagcaag aacaagcctg aagagcaagc
    12781 tcaagaggtt gcagacaagg ctgaagaaac gatagccgat ctcgatacac caattggaaa
    12841 aaatactcaa gtagaggagg aagtctctca agctgaagtc gaactggaaa gccagcaaga
    12901 agagaaaatt gaaactcctg aaggcggtga agcgagaaca gaaatagaag aaaagaaggc
    12961 atctaattct actgaagaag agccagacct ttctaaagaa acagaaaaag tcactatagc
    13021 tgaagagagc caagaagctc ttcctcagca aaaagcaacc acgaaagagc cacttcttat
    13081 cagtaaatct ttagaaagtc cttatatccc cgaccaagct ccaaaatcta gggataaatg
    13141 gaaagagcaa gtgcttgatt tttggtcttg gctagtggaa gcgatcaaat ctcctacaag
    13201 taagttggaa acaagtatca cacacagtta cacagccttt ctcttgctca ttctgttttc
    13261 tgcatcttcc tttttcttta gtatctatca catcaaacat gcttactatg gacatatagc
    13321 aagcattaac agtcgcttcc ctgagcagct agctccttta actctttttt ctatcatctc
    13381 tatcctagta gcgacaacac tcttcttctt ttcattcctc ttgggtagtt tcgttgtgag
    13441 acgatttatc caccaggaaa aggactggac gctagacaag gttctccaac aatatagtca
    13501 actcttggca attccaatct tcctcactgc tattgctagt ttctttgctt tctttgatag
    13561 cctacgattt acggccctct tgtgtgtgat tagcattgga atcattctgc ttgccagtct
    13621 ccatatcatt acaagaccaa gtcaagcaag tgaaaccgac tccttctatc aattattctt
    13681 gtctgtcctt gtgaacggag ttattatcct cctcttcttt gtagctgaag tggcactgat
    13741 tggagattat cttcgtatct tggcctttct ttaaaattca atcctgtcat ccttggcagg
    13801 attttttcta tgtcaaaaaa gcaagtcgat tgacctgctt ctgctttact tttcttgtgc
    13861 taatgtttta aaatctttgt ctaatatggg ttgcacttct atttgattgg cgtctagttg
    13921 gtggtaaaaa gatgggggta atacctctaa gaattttgca tagtccaagc gagcaatggc
    13981 ttcatagtct tctggtttag agccatctcc tgtgatttga accaagtcaa tctcccactg
    14041 cacttcctta tccaccaatt gctcaatata ggctgcagga acaaacggtt ctctcggtaa
    14101 aactgtcttg actccttgag ctagatggta aacgccatgc acttttcctt ctccatcctg
    14161 atagaaggaa actctcgcaa agtaggcatc tgtctcttga accgcacgca cacgcgcttc
    14221 aaactgagcc aaaccatctg cctctaaaaa gcttttcaag ttctcacgac taaagaaaat
    14281 aggattaaaa attccttggc aaatcgtaaa acgagctaca gggtctgcta gataggcttg
    14341 aatacttgct tggaaataag cttcaaaatc aaaaccatct agcccttcaa tatcagtccc
    14401 tgtataagca agcaaagcat ctaaaaatga cttgataatc tgccagtctg tctctgttag
    14461 taggtcagga agatcgacac ggtaagcctt ttgaccatca gcataactga ccttaaaaag
    14521 aaattgtgat tgccccacta tcgcacactc aatatactcg agacgattaa gaggctgacg
    14581 gagatagact acatcataga tatgtgactc caaaccgtct agcaatgcca agatcgactt
    14641 ggcattcaaa atctcttgtt ctcctaaaat gctctgttta tttggaataa aaaatgtttt
    14701 cgccataact ggactccttt gaaatcgctt acatacagta taccttattt ttctgaaaga
    14761 tacagaaaca aactttagat ttttgagtac aaagcataga aaaacagccc ctgaggactg
    14821 tttgatctta tacagtagct gcttgatcca agctttcacc gatagcggct aggcgctcga
    14881 tcacttcagc ttgtgtcaat tcattttttg aaacatagcg gttacgtggg tgaacacggc
    14941 actcgtgtga gcatccacga aggtacttgt cttcattttc ttctgatgtc aagatacgac
    15001 ggttacagaa gggatttcca cagttgacat aacgttcaca tggtgttcca tcaaaccagt
    15061 ctttccctac gatggttggg ttgacatggt tgacatcaac ggcgatacgc tcgtcaaata
    15121 cgtacatttt tccatcccaa agctcacctt gaacttctgg gtctttaccg taagttgcga
    15181 ttcctccgtg caattggccg acatctttgt agccttcacg gaccatccag cctgagaatt
    15241 tctcacagcg aacgccacct gtacagtaaa ccacgacacg cttgtccatg aatttttcct
    15301 tgttatcacg gacccattgt ggtaactcac ggaagttgcg aatatctggg cgaatagctc
    15361 cacggaaatg tcctaggtcg tactcataat cgttacgtgt gtcaaggaca acggtatctt
    15421 tatcaagaag cgcttctttg aactcttttg gagacaagta agcacctgtt gtttcaagtg
    15481 ggttgatgtc attgtcaaag tcgttgtctt ccaaaccaag gtggacaatt tctttcttgt
    15541 agcgaacaaa catcttcttg aaggcttgtt cattttcttc gtcaatcttg aaccagagtt
    15601 cttccattcc tggaaggctg tgaacgtagt ccatgtattt ttgagttgtt tcatagtcac
    15661 ctgaaactgt tccgttaatt ccctcgtcag cgactaggat acggccttta agaccgattg
    15721 atttacagaa agccaagtgg tctgcagcaa attgctctgc attttcaatt ggagtataaa
    15781 ggtagtaaag taagacacga atatcttttg ccataagatt tgttctcttt tctattctta
    15841 aattttctaa attttttatt tatccatact agtatacctg cttgagaaaa cgaatgcaat
    15901 ttatttgact ggaaattgta gaggattctc gaaatttcct gaatggttaa aataaggaca
    15961 agagaaaaca tggatatcta tatccttgtg ccaaaaaaac cactgccctc cccagaccaa
    16021 cctgaggaaa gcagtgattc ttattttagg agttaggaat gaatacacga aatcaattta
    16081 gctgattatt ttttgttttt caagaattca tcgtattgtt tttgcatttc gttcaatact
    16141 ttttcgtagg caccttcaga tttcaatttt tccatcaatt ctggaatcgc tttatctggg
    16201 tctacagtac cagtgttgat agctgtatca aattgttgca ttgtgttagc aatagctgag
    16261 atttcagatt tcacattgtc agtattgaag ataaatccaa gcgctggaga ttctttagct
    16321 tctgccaatt ctttcttaga attttcgatt tgttggtctg taacgttttc gttgatgtaa
    16381 aggatccagt tgttaccagt gttccatcca cccatgtgag tgtttccttt gtagccatca
    16441 agaacgcgaa cacggttttc tttaccttca attttttccc agttcttgcc ttctggaccg
    16501 taaacaagac cgttcaaaag ttctgggttc gtattcaaga ggttcaagat ttccattgat
    16561 ttttctttgt tcttagagtt gtttgagatg acaaagttag caacttgtgt tgtttggttt
    16621 ttcttgatga agttagtgat tggtttgatt tggatatctt tgttggcaac acgtgaaagc
    16681 aagctgttac cgtagtcagc tggtcctact gtttcttcac gaacgaacca agtatcttgt
    16741 tgaaggtcaa atgaagtatc gcttgttgcg acgtcttttg gaatgtagcc agcttcatag
    16801 aatttgtgaa gagtcttcaa gtgttctttg aaacgaggca cttcgtaacg gtttacaact
    16861 ttagtagtat cgccttcaag gtcaataacg aatggaagcc cgtttgctac tgggtagtca
    16921 aaattatcag atgggatgaa aactttacca atagcaaatg gtactacgtc tggagctttt
    16981 tctttgattt gtttcaagac tggctcaaga gtttcgtaag aagtaacacc tgaaatatcg
    17041 ataccatatt tggcaaggag agttccgttg aaggcaaagt tttgagatga tgctacgttg
    17101 gctgcaactg gtacagcata aatcttacca tttacagtat tacccttgat ataagctgga
    17161 tcaagtgcct tgtaaaggtc tttaccttct tttttgtaca attctgtcaa gtcagcataa
    17221 gcaccttttt gagcatttac aatatagtta tctgcaaagg cgatatcata gttttcacca
    17281 gatgatgtga taactgacat tttcttacca tagtcacccc agccaaggta ttggatatcc
    17341 aatttggcac caactttttc ttcgatgatt ttgttggcat ttgctaacaa ttcatccaag
    17401 ttatctggtt tgtcaccgat ttggtacatt ttgataacag gtttgtcacc tgaatcagca
    17461 gcttttttgc tgttacctgt caaatttcca caagcagcaa gacctgcagc cagagcgact
    17521 acactagcag atgcaaaagc atattttttc cagtttttca tgataaaaac tccttttttt
    17581 atttttaaac ttataaacaa tgtaatgatc ttaacttcac acaagggaag ttgggaactg
    17641 agaaatgttc tttttcaata agcactattc tttcacacca ccgatagtca aaccttttac
    17701 aaagtagcgt tggaagaatg gatacaaaat cgcgattgga agggttgcta ccacaaccat
    17761 ggccatacga cctgtttctt tcggtagagc aactcccagt tgaccagtca agccgaccgc
    17821 tttggcaatg tagtccatat tttgttggat ttgcatgagc aaatattgca atggatacaa
    17881 gttatcactc ttgatgtaaa gaagggcgtt gaaccagtca ttccagaaac caagagctgt
    17941 caaaagcgtg atggttgcga tacctggtag tgacaatggc aaacagattt ggaagaaaat
    18001 ccgagcctca ctggcaccat cgatacgagc cgattcgaga atggcttctg gaatggtctt
    18061 cttgaaaaaa gaacgcatca agatgatatt gaatggtgag agaagcattg gaacaatcaa
    18121 ggcccaaaca gtgtcaccaa gctgaagtac acgggtcacc atgatataac ctggtaccaa
    18181 accagcgttg aacaacatac tgagaaggac gaagatggta aaaaatctgc gatacttaaa
    18241 ggttgtccgt gaaatagcgt aggcataggt tgttgtgata aagacatttg tcaatgtccc
    18301 aactacggtt acaaagacag agatgaagag ggcttgtagg attttatcct taaactgtgc
    18361 caaaaactca aaaccgtcta agccaaattg ggatgggaag aagctatagc cgtattggag
    18421 gaggcttttc tcgtctgtca ctgaaataat gataacgaat acaaaaggta ggatacaaga
    18481 gagggcaatc aaacccgaaa tgatactgaa gaagatatct gctttcttac tgaaggagtg
    18541 aatgccgaca ttatcaattt tttctttttt aattttcttt tttgccatat tctcctcctt
    18601 tctagaacaa agctgagttt ggatcgactc gtcttgcaag caagtttgat aggataacca
    18661 gaatcaaacc aacaacggat tggtaaagac cggctgctgc agccataccg atatctgctg
    18721 tctgagtcaa accattaaag acatatacgt ccaaaacgtt ggttacattg taaagctgac
    18781 cagcattgtg tgggatttga tagaagagac cgaagtctgc gcggaagata tttccgactg
    18841 caaggatggt caatacagtt acaagcggag tcaactgagg aatggttacg ttgcgaatac
    18901 gttgccactt gctagctccg tccactgtcg ctgcttcgta gtaggttgga tcaattccca
    18961 tgatcgtcgc atagtacatg acactgctat atccaaagcc tttccaaata cctaggaaaa
    19021 gtaggagata gggccagatg cccaggtcag cgtagaaatt gacttctttg agaccaagac
    19081 tttccaatag atgattgaac acccctttat caatatttag gaaggcatct gtaaagaaac
    19141 tgatgataac ccaagacaag aagtaaggga acaacataga agtttgaaaa atcttcacca
    19201 ttctcttaga acggagctcg ctgaggataa tggcaatccc tacagataca actaaaccta
    19261 gaaagataaa gccaagattg tagaggacag tatttcgtgt gataataaag gcgtctcttg
    19321 aactaaataa gaatctgaaa ttatcgagtc cgacccattt actatttatg atactatcta
    19381 tgaaaccatt actggtcatg tggtagtctt tgaaggcaac cacgttccca aatactggaa
    19441 tgtaaaagaa tagaatcaac cagagtgccc ctggcaaaac catcaagaga aagatccagt
    19501 tgtctctcaa tgtttttgaa aactttttca taatttcctc cctttttatt ttgatatcca
    19561 tctaaaaatt cttttttaga cttttgataa cgattacatt attagtatac tcctatttgc
    19621 aggttaggtt aaactcctaa ttatagaaaa aactccacaa attatgtagc agatttaaaa
    19681 ctttatcacc actatcaaac aaatgtccta aatcaattgt ttattttatc tctattagcc
    19741 cagtgatggc gtcactctgt tataagcatc caacaacggg gtatactgaa aaatctccag
    19801 actagggaac tcagcgatag ttcctaatct ggagattttt aatatgttat taggcgtttg
    19861 ctttcaactt agcaataacc tctttaagat tatcaatcaa ctctgcttca gtatgctcag
    19921 agcctttttc atctgccaag aacaaaactg ctttttgaag ttcttcttga gagttttcaa
    19981 ggacatcctt atctgctgtt tctttgcccg ctgtttgtcc tccatcctta gttgctgact
    20041 ggctttcctt aatggactct agggaagcaa tggcatcttt gactgtttgc aagatatcac
    20101 gtaaaccttg ctctgtcaaa ctatcatctg caaaagcttt cttagcctct gccaaaacca
    20161 gacgtgctga atctgtggta ggattcgata cacctgtcaa tgatctcaaa agattttcta
    20221 aagtttgagt ctgcttacta atactagact aaaatcaaaa agcattatat aacagtgata
    20281 tgaaatcaac taaagaagaa atccaaacta tcaaaacact tttaaaagac tctcgtacag
    20341 ctaaatatca taaacgcctt caaatcgttc tatagtaaaa tgaaacaaga acaggacaaa
    20401 tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt gtactattct
    20461 agtttcaatc cattatattt cgtctgatgg gcaaatctta taaagagatt atagaacttt
    20521 tatagtagtt tgaaataaga tgtgaacaac tctatcagga aagtcaaatt aatttataga
    20581 aatattttag cagctaaggt g
//
LOCUS       DAVBCB010000044        20170 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVBCB010000044 DAVBCB010000000
VERSION     DAVBCB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.044"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            75..539
                     /locus_tag="VQU28_001511"
     CDS             75..539
                     /locus_tag="VQU28_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HET2476847.1"
                     /translation="MGGHPGFNCLLLDDEVYEDYYLEFEKEEICSVPRPFPETGMLDF
                     QDRSPWLEGQKEIDLSYDLFSTDAVTLDELQSRTIALRSLKHDKGLKVHFAEFPNLII
                     WSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            697..1122
                     /gene="lacA"
                     /locus_tag="VQU28_001512"
     CDS             697..1122
                     /gene="lacA"
                     /locus_tag="VQU28_001512"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HET2476848.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            1153..1668
                     /gene="lacB"
                     /locus_tag="VQU28_001513"
     CDS             1153..1668
                     /gene="lacB"
                     /locus_tag="VQU28_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HET2476849.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            1679..2608
                     /locus_tag="VQU28_001514"
     CDS             1679..2608
                     /locus_tag="VQU28_001514"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HET2476850.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDDQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKLVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQESLFTGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAGLLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            2610..3590
                     /gene="lacD"
                     /locus_tag="VQU28_001515"
     CDS             2610..3590
                     /gene="lacD"
                     /locus_tag="VQU28_001515"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HET2476851.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKILVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            3824..4660
                     /locus_tag="VQU28_001516"
     CDS             3824..4660
                     /locus_tag="VQU28_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HET2476852.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENH
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALIQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            4696..5013
                     /locus_tag="VQU28_001517"
     CDS             4696..5013
                     /locus_tag="VQU28_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HET2476853.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            5015..6709
                     /locus_tag="VQU28_001518"
     CDS             5015..6709
                     /locus_tag="VQU28_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HET2476854.1"
                     /translation="MDKLIIFIEKGKPFFEKLSRNIYLRAIKDGFISSMPAVLFSSIF
                     ILIAAVPNIFGFKWSDEQLAFILKPYNYSMGILALLVAGTTAKSLTDSVNTRSMEKTN
                     QINYMSTFLAAVVGLLILAADPIEGGFANGLLGTRGLLTAFLAAFITVNIYKVCIKNN
                     VTIRLPEEVPPNIAQVFKDVIPFALSVLSIYGLDLIVRNIFGTNVAESVGKILAPLFS
                     ATDGYIGLAIVFGAYAFFWFVGIHGPSVVEPLIVAISYANIEANVQLVQAGMHADKIL
                     NPVTQTFVVTMGGTGATLVVPFMFMWLCKSKRNRIVGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFIPFVTAPIINVWIMKFFVDVLQMNSFSIILPWTTPAPIGIVMGTALAPLSF
                     VLAITLIIIDTLIYYPFVKVYDHQILEEERSGKSNDELKEKVAANFNTAKADAILEKA
                     GVDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLP
                     EFDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            6918..8324
                     /gene="lacG"
                     /locus_tag="VQU28_001519"
     CDS             6918..8324
                     /gene="lacG"
                     /locus_tag="VQU28_001519"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HET2476855.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPLDPKNPSDVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            8367..8549
                     /locus_tag="VQU28_001520"
     CDS             8367..8549
                     /locus_tag="VQU28_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HET2476856.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLFLNFINPEE
                     WDTISFDSDFMPFQQS"
     gene            complement(8827..9588)
                     /locus_tag="VQU28_001521"
     CDS             complement(8827..9588)
                     /locus_tag="VQU28_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HET2476857.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNVSNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(9770..10732)
                     /gene="nrdF"
                     /locus_tag="VQU28_001522"
     CDS             complement(9770..10732)
                     /gene="nrdF"
                     /locus_tag="VQU28_001522"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HET2476858.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(10920..13079)
                     /gene="nrdE"
                     /locus_tag="VQU28_001523"
     CDS             complement(10920..13079)
                     /gene="nrdE"
                     /locus_tag="VQU28_001523"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HET2476859.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(13160..13378)
                     /gene="nrdH"
                     /locus_tag="VQU28_001524"
     CDS             complement(13160..13378)
                     /gene="nrdH"
                     /locus_tag="VQU28_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HET2476860.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            13729..13992
                     /locus_tag="VQU28_001525"
     CDS             13729..13992
                     /locus_tag="VQU28_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HET2476861.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            13998..15731
                     /gene="ptsP"
                     /locus_tag="VQU28_001526"
     CDS             13998..15731
                     /gene="ptsP"
                     /locus_tag="VQU28_001526"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HET2476862.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            15986..18436
                     /gene="phtA"
                     /locus_tag="VQU28_001527"
     CDS             15986..18436
                     /gene="phtA"
                     /locus_tag="VQU28_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HET2476863.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            18594..>20170
                     /locus_tag="VQU28_001528"
     CDS             18594..>20170
                     /locus_tag="VQU28_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2476864.1"
                     /translation="MKINKKYLAGSVATLVLSVCAYELGLHQAQTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISEE
                     LLMKDPNYQLKDEDIISEIKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTSTNDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWLPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGNHYHF
                     IPYEQMSELEKRIARIIPLRYRSNHWVPDSRPEQPSPQSTPEPSPSLQPAPNPQPAPS
                     NPIDEKLVKEAVRKVGDGYVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLG
                     AKKTDLPSSDREFYNKTYDLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKL
                     VDDILAFLAPIRHPERLG"
BASE COUNT         6442 a         3614 c         4159 g         5955 t
ORIGIN      
        1 ttatcaatca taaactagaa ttctagtttt gcaggaagtc taataaagaa actagcaagg
       61 tcaggcctta ctttattggt ggacatccag gatttaattg tcttctactt gatgatgaag
      121 tctatgaaga ttattatcta gagtttgaga aagaagagat ttgctctgtt ccacgtcctt
      181 tcccagaaac aggtatgttg gatttccaag atagaagtcc atggctagag ggacaaaaag
      241 aaatagatct tagttatgat cttttcagca cagatgcagt gactttggat gaattgcaat
      301 ctcgaacaat tgcccttcgt tctcttaaac atgataaggg attgaaagtg cattttgcag
      361 aatttccaaa cctcatcatc tggtcaactt tgaacaaggg acctttcatt acctttgaac
      421 catggtctgg cttgtcaaca ttccttgaag aaggagatca tttagaagat aagaagaatg
      481 tttgtctctt agaagccaat caggttgaag aattagggtt tgagatagaa gttttataaa
      541 aaaagtaaaa aataaataac aaaaacaaac acaaactgtt gacttcttca aacaatagta
      601 gtatattatg tttgtgagga ctaattgact gttttttaaa gacagccaat acactgctgg
      661 aatccagcat agaaaaaata aaaaggagta tacaatatgt ctattgttat cggtgcagat
      721 gctgcaggtt tgagattgaa agaagttgtg aaagacttct tagaaaaaga aaacttccac
      781 cttgtggatg ttacagctga aggtcaagac tttgttgatg tgactctcgc tgttgctgca
      841 gaagtaaaca aagaagaaca aaaccttggt atcgtgattg atgcttatgg agctggtcca
      901 tttatggttg caactaagat caaaggaatg gttgctgcag aagtatctga cgaacgttca
      961 gcttatatga ctcgtggcca caacaactca cgtatgatca ctatgggggc acaacttgtt
     1021 ggtgatgaat tggctaaaaa catcgctaaa ggatttgtta atggtaaata cgacggtgga
     1081 cgtcatcaaa tccgggttga catgttgaac aaaatgggct aattagatta cagtaagaaa
     1141 ggtaagttaa aaatgagaat tgcaattgga tgcgaccaca tcgtaacaga tgaaaaaatg
     1201 gcggtttcag aatttttgaa atcaaaagga tatgaagtca ttgactttgg tacatatgac
     1261 catacacgga ctcactaccc aatctttggt aaaaaagtag gggaagctgt aactagcggc
     1321 caagctgatc ttggggtatg tatctgtggt actggtgttg gtatcaacaa cgctgtaaat
     1381 aaagttccag gtgttcgttc tgccttggtt cgtgatatga caacagccct ttatgctaaa
     1441 gaacaattga acgctaacgt tatcggcttt ggtggtaaga ttactggtga attgcttatg
     1501 tgtgatatca tcgaagcttt cattcatgct gaatacaaac catctgaaga aaacaagaaa
     1561 ttgattgcga aaattgaaca ccttgaaagt cacaatgctc aacaaacaga cgcaaacttc
     1621 tttacagaat tccttgaaaa atgggatcgc ggagaatacc acgactaaga ggtgacctat
     1681 gattttaaca gtcacaatga acccatccat tgatatttcc tatcccttgg atgagttgaa
     1741 gattgatact gtcaatcgtg tggtggatgt aaccaaaacg gctggtggta agggacttaa
     1801 tgttacccga gtactttcag aatttggtga ttctgttctt gctactggtt tagtgggtgg
     1861 caaacttggt gagtttttgg ttgaacatat cgatgatcaa gtaaagaaag atttcttttc
     1921 tattcaggga gaaactcgta actgtatcgc tattctccac ggtgacaacc aaacagaagt
     1981 tcttgaaaaa ggtcctgaag tattggaaca ggaaggtcaa gactttttgg aacatttcaa
     2041 aaaactcttg gagtcagttg aagtagtagc catctcaggt agtctgccag ctggccttcc
     2101 agttgattac tatgcgagct tggtagagct tgctaatcaa gctggcaagc tagtagtctt
     2161 ggactgctct ggtgcagcac ttcaggcggt tcttgaatca ccccataaac cgacagtaat
     2221 caaacccaat aatgaggaat tgtctcagct tctaggaaga gaagtttctg aggatttgga
     2281 ggaattaaaa gaagtccttc aagaatcttt gtttacaggg attgaatgga ttatcgtttc
     2341 acttggtgcc aacggtactt ttgccaaaca tggtgacact ttctacaagg tagatattcc
     2401 tagaattcag gtggtaaatc ctgttggatc tggagactct actgtggcag gaatttcttc
     2461 aggacttctt cacaaagaat cggatgcagg attactcatc aaggcaaatg tccttggtat
     2521 gctcaatgct caagaaaaaa tgactggtca tgtcaacatg gccaactatc aagctctata
     2581 tgatcaatta atagtaaaag aggtataaaa tggctttaac agaacaaaaa cgtgtacgct
     2641 tagaaaaact ttctgatgaa aatggtatca tctcagctct tgcatttgac caacgtggtg
     2701 ctttgaaacg cctcatggct caacaccaaa cagaagaacc aactgtggcc caaatggaag
     2761 aacttaaaat cttggtagca gatgaattga ctaaatacgc ttcatctatg cttcttgacc
     2821 ctgagtatgg acttccagca actaaggctc ttgatgaaaa agctggtctt ctccttgctt
     2881 atgaaaaaac aggttatgac acaacaagca caaaacgctt gccagactgc ttggatgttt
     2941 ggtctgcaaa acgtattaaa gaagaaggtg cagatgcagt taaattcttg ctttactatg
     3001 atgtagatag ctcagacgaa ctcaatcaag aaaaacaagc ctacatcgaa cgcatcggtt
     3061 ctgagtgtgt ggctgaagat atcccattct tccttgaaat ccttgcttac gatgaaaaaa
     3121 ttgcggacgc aggttctgta gaatacgcta aagtaaaacc acacaaagtt atcggtgcta
     3181 tgaaagtctt ttcagaccca cgttttaaca ttgatgtctt gaaagttgaa gttcctgtta
     3241 acattaaata tgttgaaggc ttcgctgagg gtgaagtggt ttacacacgt gaagaagcag
     3301 cagccttctt caaagcgcaa gatgaagcaa cgaacttgcc atacatctac ttgagtgctg
     3361 gtgtatcagc taaactcttc caagatactc ttgtatttgc tcatgaatca ggtgcaaact
     3421 ttaacggagt tctttgtggt cgtgctacat gggcagggtc agttgaagct tacatcaaag
     3481 atggtgaagc agcagctcgc gaatggcttc gcacaactgg atttgaaaac attgatgagc
     3541 tcaataaagt tcttcaaaca acagcgactt catggaaaga acgtgtgtaa gaaagtcctc
     3601 ctagtttagg aacatgaatc taaaaaaatt taaaaaaagt tgtatgtaaa ggtttacaaa
     3661 ataacttact tgtgctatac ttaaatcaca agttaataca aggtgagtgt tactaagtaa
     3721 tattaggcat gatcacaggt gaattagaaa tcagctgatt ttctagttca tttgtggtca
     3781 ttttttgtac tcatatacct ttaagatata aaaggaggtt gacatgtatc gaattctaaa
     3841 tccaatgaat cacaatgtct cgcttgtcag aaatgataag ggagaagagg tgattgtaat
     3901 tggtaagggg attgcattcg gaaagaagaa gggggatttg attgctgaaa atcatgttga
     3961 gaaaatcttt cggatgaaga ccgaagagtc cagagaaaac tttatggctc ttctcaaaga
     4021 tgttccgctt gattttatca cagtgaccta tgaaatcatt gataagctat caaagaaata
     4081 tcattatccg attcaagagt atctctatgt aaccttgaca gatcatattt actgttctta
     4141 tcaagctcta attcaaggaa ggtacaagga tagtaatctg ccagatattt ctgctaagta
     4201 tcctgtcgct tttcaaatcg caaatgaagc ttttgaaatt taccgtcaga agctagcaga
     4261 tcattttcct gaggacgaaa ttattcggat tgcttatcat ttcattaatg ctgaaggtga
     4321 aaatgaagtg gaacttgtgg agtcgattga taagaggaaa gaaattctca ggaatgttga
     4381 agaagtttta acggactatg caattcaacg aactaaaaag aataaccatt tctatgatcg
     4441 ctttatgatc catttgaatt atttcttgga ttatttagac agatctagag atgataacca
     4501 atcacttctg gatatggaag atcatattaa acaatcctat ccaaaagcct tcgagattgg
     4561 ttccaagatc tatgatgtga ttacgcaaca tacgggtctt gatttgtata aaagtgaacg
     4621 agtttatcta gttctacata tccaacgttt attgtcataa aaatttattt aaaactatat
     4681 aaggagaatt ctatcatgaa tagagaagaa gtaacattgt taggttttga aatcgtagcc
     4741 tatgctggcg atgctcgttc aaaactattg gaagccttga aggctgctga agctggtgat
     4801 tttgaaaaag cggacgctct ggtagaggaa gctggtagct gtattgcaga ggctcaccac
     4861 gcgcaaacaa gtctattgac taaggaagct tctggtgagg acttagcgta tagtgtgacc
     4921 atgatgcatg gtcaagatca cttgatgaca actatcttgt taaaagacct aatgcaccat
     4981 ttaattgaac tttacaagag aggagttaaa taaaatggat aagttaataa tttttattga
     5041 gaaaggaaag cctttctttg agaaattatc gagaaatata tacttaagag cgataaaaga
     5101 tggttttatt tccagtatgc cagcagtatt attctcaagt atttttatac taatagctgc
     5161 tgtgcccaat atttttgggt ttaaatggtc tgatgaacag ttagctttta ttttgaaacc
     5221 ttacaactac tcaatgggga ttttagctct cttagtagct gggacaactg caaaatcgtt
     5281 aacagattca gttaatacac gtagtatgga gaaaaccaac caaatcaact acatgtcaac
     5341 ttttttagca gctgttgttg gattgttaat actggcagct gatccaattg aaggtggatt
     5401 tgctaatggt cttttaggta ctagaggatt acttactgca tttttggcag cttttattac
     5461 agtaaatatc tataaagtat gtattaaaaa taatgtaacc attagacttc ctgaagaggt
     5521 tccaccaaat attgcacaag tttttaaaga tgttattccg tttgccttat ctgttctttc
     5581 aatatatgga ttggatttaa ttgttagaaa tatttttggt acaaatgtag cagaatctgt
     5641 tggtaaaata ttagcccctc tattttcagc tactgatggt tatatcggtt tagctattgt
     5701 atttggtgct tatgctttct tttggtttgt tggaattcat gggccatctg ttgtagaacc
     5761 tttaattgta gctatcagtt atgcaaatat agaagctaat gttcagcttg tgcaagctgg
     5821 tatgcacgca gataaaattt taaatccagt tactcaaact tttgttgtaa caatgggtgg
     5881 tactggtgca acactagtag ttccatttat gtttatgtgg ttatgtaaat caaaaagaaa
     5941 tagaattgtc ggacgtgcgt ctgtagttcc tactttcttt ggcgtaaatg aaccaatcct
     6001 atttggtgct ccaattgttt taaatcctat cttctttatt ccgtttgtta ctgctcctat
     6061 tatcaatgtt tggattatga aattttttgt agatgttcta caaatgaata gtttcagtat
     6121 tattttacct tggacaacac ctgctccaat tggtattgtt atgggaacgg cgctggctcc
     6181 attgtcattc gtcttggcta taactttaat cattatcgac actttaatct attatccatt
     6241 tgttaaagtt tatgaccatc aaattttaga agaagaacgt tcaggtaagt ctaatgatga
     6301 attgaaagaa aaagttgctg caaacttcaa cactgcaaaa gcggatgcta ttcttgaaaa
     6361 agcgggtgtc gatgcagcac aaaataccat cactgaagaa acaaatgtcc tcgttctctg
     6421 tgcaggtgga ggaacaagtg gtctccttgc aaatgctttg aataaggcag cagcagaata
     6481 caatgtccct gtgaaagcag cagcaggcgg ctatggtgct caccgtgaaa tgttaccaga
     6541 gtttgatctt gttatccttg cccctcaagt tgcttcaaac tttgaagata tgaaagcaga
     6601 aacagataag ctcggtatta aactagcgaa aacagaaggc gctcaataca tcaaattaac
     6661 tcgtgatgga aaaggtgctc ttgcattcgt acaagcgcaa ttcgattaag gctagagact
     6721 ctgaaatagt ctcctatcgt tacggaaatc gctatggcga atttcctatt attaattcgt
     6781 cggtaaaaag atatcgtttt tacctcctca tgtcacaatt cggtgacttg gtacaagaag
     6841 tgagatggag aaggatggct cactgactcc tctcctctca cttttacttt atttaaatca
     6901 agaaataggt gaaaaaaatg acaaaaacac ttccaaaaga ctttattttt ggtggcgcaa
     6961 cagctgccta tcaagcagaa ggtgctacac atactgatgg taaaggacca gtagcgtggg
     7021 ataaatatct tgaagataac tactggtaca ctgcagaacc agccagtgat ttttacaatc
     7081 gatatccagt tgacctcaag ctagcagaag agtatggtgt caatggtatt cgaatttcta
     7141 ttgcttggtc acgtattttc ccgactggtt acggccaagt aaatgctaaa ggtgttgagt
     7201 tttatcataa tttatttgca gagtgtcaca aacgtcatgt tgagcccttt gtaactcttc
     7261 atcactttga cacgccagaa gctctccact caaatggaga cttcttaaac cgtgaaaata
     7321 tcgaacattt tgtagactac gctgccttct gttttgaaga atttccagaa gtaaactatt
     7381 ggacaacctt taatgaaatt gggccaattg gtgatggtca atacttggtt gggaaattcc
     7441 ctccaggtat tcagtacgac cttgccaaag tcttccaatc gcaccacaat atgatggtgt
     7501 ctcatgcgcg tgcagttaag ctatataaag ataaaggcta taaaggcgaa attggtgttg
     7561 ttcacgctct gccaactaag tatcctttag atcctaaaaa tccgtcagat gttcgtgcag
     7621 ctgagttgga agatattatt cacaataaat ttatcttgga tgcaacttat ctaggtcgct
     7681 attcagctga aacgatggaa ggtgtcaacc atatcttatc agtcaatggt ggtagcttgg
     7741 atcttcgtga agaagatttt acagcattag aagcagcaaa agacttgaat gatttccttg
     7801 gaatcaacta ctatatgagt gactggatgg aagcctttga tggagaaact gaaattatcc
     7861 ataatggtaa gggtgagaaa ggaagttcta aataccaaat caaaggtgtt ggtcgtcgtg
     7921 tagctcctga ttatgtacca cgtacggatt gggattggat tatctaccct caaggtttat
     7981 atgaccaaat tatgcgtgtg aagaaagatt atcctaacta caagaagatt tacatcactg
     8041 aaaatggtct cggctataaa gatgagttcg tcgataacac tgtttatgat gatggacgta
     8101 ttgattatgt gaaacaacac ttggaggtct tgtctgatgc gattgcagat ggagcgaatg
     8161 taaaaggtta cttcatttgg tcattgatgg acgtcttttc atggtcaaat ggttatgaaa
     8221 aacgttacgg tctcttctat gtagactttg aaactcaaga acgttatccg aagaaatcag
     8281 ctcactggta caagaaagta gcggaaactc agattataga ctagtagaat tagtcattag
     8341 atatagaatt ttagtgagtc aaaaagatgt tcaaagattt tatccaatct atttatgaaa
     8401 aagtttatat tataaatttc gaaaaatgct ctcaaatacc gtgtttgacg agtgaagaat
     8461 tgaaaagtct atttttaaat tttatcaatc ctgaagaatg ggatactatc tcctttgatt
     8521 cagattttat gccatttcaa caatcgtaac caatttctca aaaaagttaa atcttatatt
     8581 tagtactctg caaaactctt atctaatcac gttgcttata ctcaatgaaa atcaaagagc
     8641 aactttaaac taggaagcga gtcgcagatt tctcaatgca tagctttgag gaattgggca
     8701 aaaagtcttt gatatagaaa aagcatagta tcaggtgttt caacacctga tactatgctt
     8761 tttattgtgg gaagatttac tttttttctt ctgaaattga gttgttaccc aggctctttc
     8821 agtttattaa ggcttgatga ctttaatgtg tttagatagc ttaaaaagga ttgaatcact
     8881 tagtttagaa tctgaaacaa tagtatcaag atttgataca ttataaaaag tataaaaatc
     8941 aaacttattg aacttactat gatctgcgag taaatatttt ttattagaat tatttaaagc
     9001 gatgcgttga gcctctccct cttcctcgct aaaagtagct agagctccgt tttgaatacc
     9061 attacagcta acgaaagctt tagaaaattg gagattagag agattttgta gggtcaatgt
     9121 gccaacaaaa gcacctgtaa tatcgcgata atttccacct atcaaaatca aatctgttaa
     9181 ttttcgttcg cttaaaatca gaaaaacagg tagactgttg gttacgacgc ggatattgtc
     9241 aataggcaac tcacgcgcaa aaaactctaa tgttgttcct ggtccaatga aaatagtttc
     9301 tctttcttct actagactgc ctgcaaaatg ggctatttct tgtttttctg ccgtttggag
     9361 ggcttgtttt tcaatatttg atcgctcatt agtcaaaagg gagttggttc gaagtttttc
     9421 agctccacca tgcacacgaa tcagcaaatc tttatcagct aattcctgta aatagcgcct
     9481 tgcagtcata tctgaaacgg ctatttcgtc cataatctgt ttaactgtta tggttccttt
     9541 actatttact atctctaaaa ttttggctaa tttttcttgt ttgagcatat tatcacctcg
     9601 tttcctacta ctatcttacc ataaacaaac tcatcattca aatacaaaaa caacaaaatg
     9661 aaacaaaaac aaaaatatcg aagtttgttt tcaaaacttt cgatattttt gttgggttat
     9721 aactttgatg tttctagttt actttttgat gattgagagt gatggagaat tagtctaaac
     9781 cgtagttata gtcatcgtct tgcatggctt caacttcgcc aagaaggtaa ccatttccga
     9841 cttgagagaa gaagtcatgg ttggaagttc ctgttgaaat accgttcata acgattgggt
     9901 tgacatcttc agctgaatct gggaaaagtg gatcttgtcc catgttcatg agagctttat
     9961 tggcattgta gcgaaggaag gttttaacct cttcagtcca accaacaccg tcataaagac
    10021 tctctgtgta gccttcttca ttttcataaa gagtatagag taggtcgtac atccattctt
    10081 tgagtttttc ttgctcttct tcaggcaatt cattgaaacc aagttggaat ttgtaaccaa
    10141 tgtaggttcc gtgaacagac tcgtcacgaa taatcaattt aatgatttct gcaacgttgg
    10201 ctagtttgtt gttaccgaga tagtagaggg gagtgaagaa accagagtag aagaggaagg
    10261 tttcgaggaa gacgctggca actttctttt caagtgggct gccgtttagg tagatttcgt
    10321 tgacaatctc agccttcttt tgtaggtaag gattggtatt ggtccattcg aaaatttctt
    10381 caatctcagc cttagtattc aaggtagaaa agattgatga gtaagattta gcgtggacag
    10441 attccataaa ttggatgtta ttgaaaacag cttcctcatg tggtgtacgg atgtctgcgc
    10501 gaagggcttg aaccccagtt tcagattgca tagtgtcaag aagggttaaa ccaccaaaaa
    10561 cttttccgac caagtctttc tctttgttag atagctttct ccagtcatcc aagtcgtttg
    10621 ataagggaat acgtgtatcg agccaaaatt gctccgtcag tttttcccaa gttgatttgt
    10681 cgatgacatc ttcgatggca ttccagttaa tggctttgta gtaagtttcc atttaaaatc
    10741 tctttctgtg tttagtattg cgaactcaca attatttcta ctttaccata attctatagg
    10801 agtatcgcac aaaaagtcgg aagcccgact tttaaaatgt tacataaatt atgttatgac
    10861 atagtagatt tgattttatc agtgctgctt agggaaaaat aatgtttcta tgctagaaac
    10921 taaatcacac agctttcaca ttggttggcg ccgacttctc caccgtcatc tgtaaaggta
    10981 cggacgtagt agatagactt gattcccttg ttaaaggcat agttacgaag gatggacaag
    11041 tcacgtgtcg tttgtttatt ttccctcttc cattcgtaaa ggccttttgg aatgtcacta
    11101 cgcatgaaga gggtgagtga aagtccttga tccacgtgtt cagtcgcagc agcgtaaaca
    11161 tcgatgactt tacgcatatc catatcgtag gcagaagtgt agtaaggaat ggtttctgta
    11221 gacaagccag cagcagggta gtagatttta ccaattttct tctcttggcg ttcttcgata
    11281 cgttgcgtaa tcgggtggat agaagcagaa acgtcgttga tatagctgat agaaccattt
    11341 ggcgctacag caaggcgatt ttggtggtaa agaccatctt cttgaacctt gtcgcgaagt
    11401 tcagcccaat cagcaacacc agggataaag acatttttga agagttcttt aacacggtct
    11461 gatgttggaa caaattcacc agttacatac ttgtcaaagt aacttccgtt agcatagtct
    11521 gatttttcaa agttgtggaa ggtaatacca cgttcacgcg caatattgtt tgactctacc
    11581 aaggtccagt agttcataag cataaagtag atgcttgtaa attcaacaga ctcaggtgaa
    11641 ccatattcaa tgagttgttg ggcaaggtag ctgtgcagtc ccatggcacc gagaccaaag
    11701 gtgtgggctt ggctatttcc atggtcaatc gttggtacag ctacgatatg tgaactatct
    11761 gtaacgaaag taagggcacg aaccatagca cggatagaac gaccaaaatc aggtgaagtc
    11821 atcatgttaa ccacgttggt tgaacccagg ttacatgaaa catctgttcc catttgaagg
    11881 aattcttgag catcgttgat caagcttggt tcttgaactt gaagaatctc agaacacaag
    11941 ttactcatga taatctttcc atcaacagga tttgcacggt tagccgtatc gatgttgact
    12001 acataaggat agccagactc ttgttgcaat ttagagattt cagtttccaa atcccgcgcc
    12061 ttgatttttg tcttgcgaat atttggattt gcgaccaatt catcgtattt ttcagtaatg
    12121 tcgatgtaat tgaatggcac accgtattct ttttctacag agtaagggct gaagaggtac
    12181 atttcttcat ttttacgagc caattcgtag aatttatcag gtactacaac accaagtgat
    12241 agagtcttga cacgtacttt ttcatcagcg ttttctttct tagttgaaag gaaagcgatg
    12301 atatctgggt gaaagacgtt gaggtagaca acaccggcac cttgacgttg ccccaattgg
    12361 ttggagtaag agaagctgtc ttcaaaaagc ttcataacag gaacgacacc tgaagcagct
    12421 ccttcatagc ctttgatagg tgcaccagct tcacgaaggt tgctgagggt aattcccaca
    12481 ccaccaccaa tacgtgaaag ttgaagagct gagttgatag aacgtccgat agagttcata
    12541 tcatccgtca cttggattag gaaacaagat accaactctc cacggcgagc acgtccagca
    12601 ttcaagaagg aaggagtagc aggttggtag cgttggtgga tgatttcatt ggcaatatcg
    12661 attgcaacag cttcattccc atcagcgaaa taaagggcgt tgaagaagac acggtcttcc
    12721 atattttcaa gatagtattc accgtcatta gtctttaagg catattgatt gtaaaattta
    12781 taagctgcca tgaatgactt gaattggaag ttttggtctt tgataaattg agctaattct
    12841 tccaagaact ctggacggta tttcttgata aaggctgttt cgatgtagtt gtgttcaatg
    12901 aggtaattga ttttgtcttt gattgaatca aaaaccatag tgtttggaac tacattttct
    12961 ttaaagaaag catccaaggc ttccttgtct ttatgaagca tgatttgtcc attaacagga
    13021 cggttaattt cgttattaag acggaagtaa gtcacgtctt caagatgttt taatcccata
    13081 aaatttccct tatctaatta caaaagaaag gcttctaagt tagccctaaa agcagtttct
    13141 tctggatgat gtactaagat tatgctaatt gtttcagttt tcctggttgg aaacctgaaa
    13201 agacttcagt tggtgtttgg ataacaggag ctgcgctaaa accgagctct ttaacttgat
    13261 cgacgtactc aggttgctca tcaagattga tttcacgata agagacatta ttactgtcca
    13321 agaaacgctt ggtcatttta cattggacac aattgttttt agaataaacg gttaccattg
    13381 tgtaactcct cttcaaaatt taatactatc ttagtatatc agaaaataaa attttgtcaa
    13441 tgatttgaac taaatatagt ggtgttcttt tgttgttttt atatataaac acaatatata
    13501 gtgtttgtat tgtgaaatag caatgattat cctataaatt gtttttaaag aaactatatc
    13561 ctgtgttttg ttatctagga tagaagattt tcagcaagtt atctgaaagg aaattgaagg
    13621 aaaatccaga aaatgcttga aaaaaatcct agaagatggt ataatactaa attgtaaggg
    13681 ttatcacata taactcaaaa aaagaaagaa caaaaggaga gtcaaactat ggcttctaaa
    13741 gatttccacg tagtggcaga aacaggtatt cacgcacgtc cagcaacatt gttggtacaa
    13801 actgctagca aatttgcttc agatatcact cttgagtaca aaggtaaatc agttaacctt
    13861 aaatcaatta tgggtgttat gagtcttggt gttggccaag gtgctgacgt aactatctca
    13921 gctgaaggtg cagatgcaga tgacgctatc gctgcaatct cagaaacaat ggaaaaagaa
    13981 ggattggcat aagggaaatg acagaaatgc ttaaaggaat cgcagcatct gacggtgttg
    14041 cagttgcaaa agcatatcta ctcgttcagc cggatttgtc atttgagact attacagtcg
    14101 aagatacaaa cgcagaagaa gctcgccttg atgccgctct acaggcatca caagacgagc
    14161 tttctgttat tcgcgagaaa gcagtaggta cgctcggtga agaagcagct caagtttttg
    14221 atgctcactt aatggttctt gctgacccag aaatgatcag ccaaatcaag gaaactatcc
    14281 gtgcgaagaa agtgaatgca gaagcaggtc tgaaagaagt tacagatatg tttatcacta
    14341 tctttgaagg catggaagac aacccataca tgcaagaacg cgcagcggat atccgcgacg
    14401 tgacaaaacg tgtattggca aaccttcttg gtaaaaaatt gccaaaccca gcttctatta
    14461 atgaagaagt gattgtgatt gcgcatgact tgacaccatc tgatacagct caattggaca
    14521 aaaactttgt aaaagctttt gtaaccaaca ttggtggacg tacaagccac tcagctatca
    14581 tggcacgtac acttgaaatt gctgctgtat taggtacaaa taacatcact gaaatcgtta
    14641 aagacggtga catccttgct gttaacggga tcactggaga agtgattatc aacccaacag
    14701 atgaacaagc ggcagaattt aaagcagctg gtgaagccta tgcgaaacaa aaagctgaat
    14761 gggcactttt gaaagatgct caaacagtga ctgctgacgg taaacacttc gagttggctg
    14821 ccaatattgg tactccaaaa gacgttgaag gtgttaacaa caacggtgca gaagctgttg
    14881 gactttaccg tacagagttc ttgtacatgg attctcaaga cttcccaact gaagatgagc
    14941 agtatgaagc atacaaggct gttcttgaag gaatgaacgg taaacctgtt gtcgttcgta
    15001 caatggatat cggtggagat aaggaacttc cttacttcga tatgcctcac gaaatgaacc
    15061 cattccttgg attccgtgct cttcgtatct ctatctctga gactggagat gctatgttcc
    15121 gcacacaaat ccgtgctctt cttcgtgcgt ctgttcacgg tcaattgcgt atcatgttcc
    15181 caatggttgc gctcttgaaa gaattccgtg cagcgaaagc agtctttgat gaagaaaaag
    15241 caaaccttct tgctgaaggt gttgcagttg cggataacat ccaagttggt atcatgatcg
    15301 agattcctgc agcggctatg cttgcagacc aatttgctaa agaagttgac ttcttctcaa
    15361 ttggtacaaa cgacttgatc caatatacaa tggcagcaga ccgtatgaac gaacaagttt
    15421 cataccttta ccaaccatac aacccatcaa tcctacgctt gattaacaat gtgatcaaag
    15481 cagctcacgc tgaaggtaaa tgggctggta tgtgtggtga gatggctggt gaccaacaag
    15541 ctgttccact tcttgtcgga atgggcttgg atgagttctc tatgtcagca acatctgtac
    15601 ttcgtacacg cagcttgatg aagaaactcg acacagctaa gatggaagag tacgcaaacc
    15661 gtgcccttac agaatgctca acaatggaag aagttcttga acttcaaaaa gaatacgtta
    15721 attttgatta atcgaaaagt ccctgcaact cagttacagg gacttttttt gatattttaa
    15781 aaagaatttt caagaaaatc tttcttataa aaagtccaac cttgaaaaag tagtggtcag
    15841 aacaaaaaat acttaaatgg ttcataaaat tcttgacaag ttggagattt aggagtaaac
    15901 tattaaccag ttaagtaata gagaggagtt tctgcaattt agaaatgaat tgcaactaga
    15961 aatatcaaat agaaagagag tttcgatgaa aattaataag aaataccttg ttggttctgc
    16021 ggcagctttg attttaagtg tttgttctta cgagttggga ctgtatcaag ctagaacggt
    16081 taaggaaaat aatcgtgttt cctatataga tggaaaacaa gcgacgcaaa aaacggagaa
    16141 tttgactcct gatgaggtta gcaagcgtga aggaatcaat gctgagcaaa tcgtcatcaa
    16201 gataacagac caaggctatg tcacttcaca tggcgaccac tatcattatt acaatggtaa
    16261 ggttccttat gatgcgattt tcagtgaaga attactcatg aaagatccaa actataagct
    16321 aaaagatgag gatattgtta atgaggtcaa gggtggatat gttatcaagg tagatggaaa
    16381 atactatgtt taccttaagg atgctgccca cgcggataac gtccgtacaa aagaggaaat
    16441 caatcgacaa aaacaagaac atagtcaaca tcgtgaaggt gggactccaa gaaacgatgg
    16501 tgctgttgcc ttggcacgtt cgcaaggacg ctatactaca gatgatggtt atatctttaa
    16561 tgcttctgat atcatagagg atactggtga tgcttatatc gttcctcatg gagatcatta
    16621 ccattacatt cctaagaatg agttatcagc tagcgagttg gctgctgcag aagccttcct
    16681 atctggtcgg ggaaatctgt caaattcaag aacctatcgc cgacaaaata gcgataacac
    16741 ttcaagaaca aactgggtac cttctgtaag caatccagga actacaaata ctaacacaag
    16801 caacaacagc aacactaaca gtcaagcaag tcaaagtaat gacattgata gtctcttgaa
    16861 acagctctac aaactgcctt tgagtcaacg acatgtagaa tctgatggcc ttgttttcga
    16921 cccagcgcaa atcacaagtc gaacagctag aggtgttgca gtgccacacg gagatcatta
    16981 ccacttcatc ccttactctc aaatgtctga attggaagaa cgaatcgctc gtattattcc
    17041 ccttcgttat cgttcaaacc attgggtacc agattcaagg ccagaacaac caagtccaca
    17101 accgactccg gaacctagtc caggcccgca acctgcacca aatcttaaaa tagactcaaa
    17161 ttcttctttg gttagtcagc tggtacgaaa agttggggaa ggatatgtat tcgaagaaaa
    17221 gggcatctct cgttatgtct ttgcgaaaga tttaccatct gaaactgtta aaaatcttga
    17281 aagtaagtta tcaaaacaag agagtgtttc acacacttta actgctaaaa aagaaaatgt
    17341 tgctcctcgt gaccaagaat tttatgataa agcatataat ctgttaactg aggctcataa
    17401 agccttgttt gaaaataagg gtcgtaattc tgatttccaa gccttagaca aattattaga
    17461 acgcttgaat gatgaatcga ctaataaaga aaaattggta gatgatttat tggcattcct
    17521 agcaccaatt acccatccag agcgacttgg caagccaaat tctcaaattg agtatactga
    17581 agacgaagtt cgtattgctc aattagctga taagtataca acgtcagatg gttacatttt
    17641 tgatgaacat gatataatca gtgatgaagg agatgcatat gtaacgcctc atatgggcca
    17701 tagtcactgg attggaaaag atagcctttc tgataaggaa aaagttgcag ctcaagccta
    17761 tactaaagaa aaaggtatcc tacctccatc tccagacgca gatgttaaag caaatccaac
    17821 tggagatagt gcagcagcta tttacaatcg tgtgaaaggg gaaaaacgaa ttccactcgt
    17881 tcgacttcca tatatggttg agcatacagt tgaggttaaa aacggtaatt tgattattcc
    17941 tcataaggat cattaccata atattaaatt tgcttggttt gatgatcaca catacaaagc
    18001 tccaaatggc tataccttgg aagatttgtt tgcgacgatt aagtactacg tagaacaccc
    18061 tgacgaacgt ccacattcta atgatggatg gggcaatgcc agtgagcatg tgttaggcaa
    18121 gaaagaccac agtgaagatc caaataagaa cttcaaagcg gatgaagagc cagtagagga
    18181 aacacctgct gagccagaag tccctcaagt agagactgaa aaagtagaag cccaactcaa
    18241 agaagcagaa gttttgcttg cgaaagtaac ggattctagt ctgaaagcca atgcaacaga
    18301 aactctagct ggtttacgaa ataatttgac tcttcaaatt atggataaca atagtatcat
    18361 ggcagaagca gaaaaattac ttgcgttgtt aaaaggaagt aatccttcat ctgtaagtaa
    18421 ggaaaaaata aactaatgaa aaatgaaagt ctcgataaag aggctttcat ttttattatg
    18481 tatatatgta aaattcttga caagcaatat taaaaagagt aaactattaa ctagttaatt
    18541 aaccggtttg ttactttata gtgaatcaaa tatacttaag aaaagaggaa agaatgaaaa
    18601 tcaataaaaa atatctagct gggtcagtag ctacacttgt tttaagtgtc tgtgcttatg
    18661 aactaggttt gcatcaagct caaactgtaa aagaaaataa tcgtgtttcc tatatagatg
    18721 gaaaacaagc gacgcaaaaa acggagaatt tgactcctga tgaggttagc aagcgtgaag
    18781 gaatcaatgc cgaacaaatc gtcatcaaga taacagacca aggctatgtc acttcacatg
    18841 gggaccacta tcattattac aatggtaagg ttccttatga cgctatcatc agtgaagaat
    18901 tgctgatgaa ggatccaaac taccagctaa aggacgagga tattatcagt gaaatcaagg
    18961 gtggttatgt gattaaggta gatggaaaat actatgttta ccttaaggat gcagctcatg
    19021 cggataatgt ccgtacaaaa gaagaaatca atcggcaaaa acaagaacat agtcagcatc
    19081 gtgaaggagg gacttcaaca aacgatggtg cggtagcctt tgcacgttca caaggacgct
    19141 atactacaga tgatggttat atctttaatg cttctgatat cattgaggac acgggtgatg
    19201 cttatatcgt tcctcacggc gaccattacc attacattcc taagaatgag ttatcagcta
    19261 gcgagttagc tgctgcagaa gccttcctat ctggtcgggg aaatctgtca aattcaagaa
    19321 cctatcgccg acaaaatagc gataacactt caagaacaaa ctggctacct tctgtaagca
    19381 atccaggaac tacaaatact aacacaagca acaacagcaa cactaacagt caagcaagtc
    19441 aaagtaatga cattgatagt ctcttgaaac agctctacaa actgcctttg agtcaacgcc
    19501 atgtagaatc tgatggcctt gttttcgacc cagcgcaaat cacaagtcga accgccagag
    19561 gtgtagctgt ccctcatggt aaccattacc actttatccc ttatgaacaa atgtctgaat
    19621 tggaaaaacg aattgctcgt attattcccc ttcgttatcg ttcaaaccat tgggtaccag
    19681 attcaagacc agaacaacca agtccacaat cgactccgga acctagtcca agtctgcaac
    19741 ctgcaccaaa tcctcaacca gctccaagca atccaattga tgagaaattg gtcaaagaag
    19801 ctgttcgaaa agtaggcgat ggttatgtct ttgaggagaa tggagtttct cgttatatcc
    19861 cagccaagga tctttcagca gaaacagcag caggcattga tagcaaactg gccaagcagg
    19921 aaagtttatc gcataagcta ggagctaaga aaactgacct cccatctagt gatagagaat
    19981 tttacaataa gacttatgac ttactagcaa gaattcacca agatttactt gataataaag
    20041 gtcgacaagt tgattttgag gctttggata acctgttgga acgactcaag gatgtctcaa
    20101 gtgataaagt caagttagtg gatgatattc ttgccttctt agctccgatt cgtcatccag
    20161 aacgtttagg
//
LOCUS       DAVBCB010000045        19051 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVBCB010000045 DAVBCB010000000
VERSION     DAVBCB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19051
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.045"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..283
                     /locus_tag="VQU28_001529"
                     /pseudo
     CDS             1..283
                     /locus_tag="VQU28_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            201..629
                     /locus_tag="VQU28_001530"
     CDS             201..629
                     /locus_tag="VQU28_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HET2476865.1"
                     /translation="MSKSTSKRYYQVAIPFTRLFCLVLVSIDYINCLSETADKNWHKS
                     DRIFVTNTGKTVHSSILSKSLQRANERLKKPIPKHLSPHIFIHITISILSENKIPLKT
                     ITDRVGHPDSEVTNSIYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1121..1531)
                     /locus_tag="VQU28_001531"
     CDS             complement(1121..1531)
                     /locus_tag="VQU28_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476866.1"
                     /translation="MPKKKIERISVIHREKILWLKWYFMRDKEKPKYSVLECKMFDAA
                     KNKDMLAYKKYATIKQITDIRVQTSEDDILTAIKEVYVYNHMNVIGACQRILFVSQSP
                     AYNKLNKWFETYSDLYFSIIPLPNMGAYHELVDS"
     gene            complement(1549..1926)
                     /locus_tag="VQU28_001532"
     CDS             complement(1549..1926)
                     /locus_tag="VQU28_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476867.1"
                     /translation="MRKYLKEIKELQELKELLSSRNTPEVIIVEGNDDLGEFFQVDGE
                     LFSDIELLENLKKWREWEVQVIVDDWCNRGLNEYETGILYFPKHEDKMDYIRFNKGLE
                     PLYHALDEPYTTISKSEWLKLLD"
     gene            complement(1868..2296)
                     /locus_tag="VQU28_001533"
     CDS             complement(1868..2296)
                     /locus_tag="VQU28_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HET2476868.1"
                     /translation="MAGNENDGLTSKQIKFIDAMLTEPTIDKACQKAGVSRATGHKYL
                     KVAAVKKTLRLKQDEMMDKTTQMLYLASSNAVSVLNDIMMDAKINPFIRTQAAKTILE
                     QSYKTHEIFGVVRQIEELRLEIEEVSKGDQRVTRTQGIIK"
     gene            complement(2340..2945)
                     /locus_tag="VQU28_001534"
     CDS             complement(2340..2945)
                     /locus_tag="VQU28_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476869.1"
                     /translation="MTFTTIKDDIKAFGKKKHGYMTGYIAKQEELHKQLQNGMIGEKY
                     AKDQLEAYKLEGDTYSRDTYNKIHAEIEKQHDLELEALKEKELSVTADDVAELTLLAS
                     MKMTKDELLGYFEKYKNKPLAIKKLWSIAEQYPEIAINLELFNAEQALESLILFFKRQ
                     LSYCHYSLLINGDKIQAVTTEMVVNSDAPELDRRLDEYLNK"
     gene            complement(3035..3208)
                     /locus_tag="VQU28_001535"
     CDS             complement(3035..3208)
                     /locus_tag="VQU28_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476870.1"
                     /translation="MTENKDNKLLEMMEKGFVLYSKNGIIKYIEIPDHGSIKLKAQDG
                     QLVYKEVTTGEQC"
     gene            complement(3807..4703)
                     /locus_tag="VQU28_001536"
     CDS             complement(3807..4703)
                     /locus_tag="VQU28_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2476871.1"
                     /translation="MKSLQSSLGNIETFKLDELCPKHEIQLTQIKGERHVVVGWNENG
                     EVIKEVRCIPPYCEQCQEEQKKQDEEDAIADNLNAKLYLQTYNVLMSNKSYVADEFKT
                     KTFDDFNAVTSEEKRFLEFAKGQVQKYLDGMRGNTLITGGTGIGKTLLSVAIAKGINE
                     GYKTKGEPKSVLFVSLTEMIKQIKEGWNYGKGATLTEFEATELMKSVDYLIIDDLGAK
                     NAVIKPKSDWEQDLLFDVLNNRENTIFNTNLDSDELKIVYNERNYSRILKGLEGNTFK
                     AFTIKDKRYSINQLKNKVKQLK"
     gene            complement(4715..5524)
                     /locus_tag="VQU28_001537"
     CDS             complement(4715..5524)
                     /locus_tag="VQU28_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HET2476872.1"
                     /translation="MAKTKVYFWLKIDKKFFDNIFIKRLKTVPGGYTMTVIYIRLMLE
                     SLESDCILYYEGYFNNLKEELALKLDVSEDDIDMTMAYFTKCGLIQIDEDKNAELPQA
                     KAMVMSETNWASYKREQRKKGKLEEVQPSLTFSNSCPTEIEKELEIEVDKEQSPTPST
                     INQDFVNLYKSFEAETGKALSPLQIQELQYMLEDFSPELIHEALKEAVSQGKANFAYI
                     KAILNRWKQDNLLTVELVRNSRAAREAKKQQTNQLEPTSYEDWIPTQEKPF"
     gene            complement(5599..5814)
                     /locus_tag="VQU28_001538"
     CDS             complement(5599..5814)
                     /locus_tag="VQU28_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476873.1"
                     /translation="MNELDISNTQAVFVTVILIGLLLYLNHRDRKKSAQLERENQQTI
                     ETPSEDLNPDYGRYIQLAGVDVWGRIE"
     gene            complement(5816..6016)
                     /locus_tag="VQU28_001539"
     CDS             complement(5816..6016)
                     /locus_tag="VQU28_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476874.1"
                     /translation="MTLDLDNMTQAEFDEAMAEIQLQSPNIFQLISDFVNKKVTSVEI
                     DDLLNMKRAEQVAYIKNYKARA"
     gene            complement(6013..6204)
                     /locus_tag="VQU28_001540"
                     /pseudo
     CDS             complement(6013..6204)
                     /locus_tag="VQU28_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841301.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6193..6483)
                     /locus_tag="VQU28_001541"
                     /pseudo
     CDS             complement(6193..6483)
                     /locus_tag="VQU28_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
     gene            complement(6653..6805)
                     /locus_tag="VQU28_001542"
     CDS             complement(6653..6805)
                     /locus_tag="VQU28_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476875.1"
                     /translation="MKIVTFKPTKQIDGGFYLPGIDILFVSDKADAKSTEDVILFISR
                     NGLNKS"
     gene            complement(6816..7082)
                     /locus_tag="VQU28_001543"
     CDS             complement(6816..7082)
                     /locus_tag="VQU28_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476876.1"
                     /translation="MEILSKEIQLQGLQLLKQTLETLVELEKQRSSKLDLISRKELMD
                     LLGISATTLDNWEDLALKRYQTPMDGAKKVFYRLSDVYLFLAIK"
     gene            complement(7293..7475)
                     /locus_tag="VQU28_001544"
     CDS             complement(7293..7475)
                     /locus_tag="VQU28_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2476877.1"
                     /translation="MLITLSLAEKVRIKRARLQLTKKAVSEQLGIKSQTLTKVENGNY
                     DASKRIYESVMTWLVE"
     gene            7537..8040
                     /locus_tag="VQU28_001545"
     CDS             7537..8040
                     /locus_tag="VQU28_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2476878.1"
                     /translation="MYKMNRIKQLRKENNLTQRELANETKIPYRTIQRWENGETDIKS
                     DAAQVLADYFGVSIPHLLGYSDLIADTDSDIKAMAYSHLLTFVDEKQIKEFEKEFIRA
                     NDNSDTFGDLGSGEKALEKYYQALCYLVPPFRDILARYSFLSLDEKKSVYNIIVGLSN
                     NLSKENK"
     gene            8114..9277
                     /locus_tag="VQU28_001546"
     CDS             8114..9277
                     /locus_tag="VQU28_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HET2476879.1"
                     /translation="MNIIEKIKKNGQRVYYASVYLGVDQLTGKKARTTVTATTKKGVK
                     VKARDAINTFAANGYTVKDKPTITTYNELVKVWWDSYKNTVKPNTRQSMDGLVRVHLL
                     PVFGDYKLSKLTTPILQQQVNKWADKANKGEKGAFANYSLLHNMNKRILKYGVAIQVI
                     QYNPANDVIVPRKQQKEKAAVKYLDNKELKQFLDYLDTLNQSNYENLFDVVLYKTLLA
                     TGCRISEALALEWSDIDLESGVISINKTLNRYQEINSPKSSAGYRDIPIDKATLLLLK
                     QYKNRQQIQSWKLGRSETVVFSVFTEKYAYACNLRKRLNKHFDAAGVTNVSFHGFRHT
                     HTTMMLYAQASPKDVQYRLGHSNLMITENTYWHTNQENAKKAVSNYETAISNL"
     gene            complement(9431..10735)
                     /gene="eno"
                     /locus_tag="VQU28_001547"
     CDS             complement(9431..10735)
                     /gene="eno"
                     /locus_tag="VQU28_001547"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HET2476880.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            10899..11345
                     /locus_tag="VQU28_001548"
     CDS             10899..11345
                     /locus_tag="VQU28_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HET2476881.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(11342..12457)
                     /locus_tag="VQU28_001549"
     CDS             complement(11342..12457)
                     /locus_tag="VQU28_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HET2476882.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIDVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENCYKIPEGVQIHILADVVSPLCGHQGATYTFGNQKGRHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(12466..12795)
                     /locus_tag="VQU28_001550"
     CDS             complement(12466..12795)
                     /locus_tag="VQU28_001550"
                     /inference="COORDINATES: protein motif:HMM:NF019887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HET2476883.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGGNNLLMLKSAELGIAFCSKEMLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(12749..13111)
                     /locus_tag="VQU28_001551"
     CDS             complement(12749..13111)
                     /locus_tag="VQU28_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HET2476884.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHC"
     gene            complement(13170..14603)
                     /gene="glgA"
                     /locus_tag="VQU28_001552"
     CDS             complement(13170..14603)
                     /gene="glgA"
                     /locus_tag="VQU28_001552"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HET2476885.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(14600..15739)
                     /gene="glgD"
                     /locus_tag="VQU28_001553"
     CDS             complement(14600..15739)
                     /gene="glgD"
                     /locus_tag="VQU28_001553"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HET2476886.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTFLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVRTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(15729..16871)
                     /locus_tag="VQU28_001554"
     CDS             complement(15729..16871)
                     /locus_tag="VQU28_001554"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HET2476887.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(16861..18789)
                     /gene="glgB"
                     /locus_tag="VQU28_001555"
     CDS             complement(16861..18789)
                     /gene="glgB"
                     /locus_tag="VQU28_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HET2476888.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEYGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFAERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDIYHLDGIRVDAVSNMLYLDYDDAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEVIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         5657 a         3987 c         3210 g         6197 t
ORIGIN      
        1 gatgacgagc gacttttttg aagcttggtt tcagaagttt ctcttaccaa cattaaccac
       61 accatcggtt attattatgg ataatgtaag attccataga atggggaagc tagaactctt
      121 gtgtgaatag tttgggcata aacttttacc tcttcctccc tactcacctg agtacaatcc
      181 tattgagaaa acatgggctc atatcaaaaa gcacctcaaa aaggtattac caagttgcaa
      241 taccttttac gaggctcttt tgtcttgttc ttgtttcaat tgactatata aattgtctaa
      301 gcgaaacagc cgataagaat tggcacaaaa gcgaccgtat ttttgttacc aatacaggaa
      361 aaacagttca tagttctatc ttgagcaagt ctctccagcg agcaaacgaa cgccttaaaa
      421 aaccaattcc caaacatctg tcccctcaca tcttcataca catcactatt agcatcttat
      481 cagaaaataa aattccttta aaaacaatca cggacagggt tggtcatccc gactctgaag
      541 tcactaattc catctacacc cacgtcacaa agaacatgaa agatgaagca atcaatgtac
      601 tggataaagt tatgaaaaag attttttaaa aagttttgtc ccttttttgc cctctaaata
      661 caaaaatagc ccttcggata aaatccgagg ggctagaaac gttgttaaat caacggccga
      721 acttttgaat ttcatggttc gggataaaat agttcactga actattttat tttttaaggt
      781 tatcataata tcaaatagtt caattaaata cgctaaatta ctaatatact ttttaccttt
      841 ttcattctaa aatgtaaagt acaaacaatt acaatatact agagggggag taaaaaaggt
      901 aataaaattg acgagttcag caggcaaggg aaatagcact tttcatggtg ttattttttt
      961 aattcagcaa tggagtagcc gcttaattga tagaaacaaa aagcacctag tttcctagat
     1021 gctagcacaa tagcccggat tcgaaccgga atctcctcaa ttaaggtgca atccctatat
     1081 actactcatt gtgctttggt tcattatatc atacaatcaa ctagctatct accaactcat
     1141 gatacgctcc catattcggt aatggaataa tactaaaata caaatctgag taagtttcaa
     1201 accatttatt cagcttgtta taggctggcg actgactaac aaataatatc cgttgacaag
     1261 ctccgataac attcatgtga ttatacacat agacctcttt aatggccgtt aaaatatcgt
     1321 cttcacttgt ctgtacccta atatctgtta tctgcttaat cgtagcgtat tttttataag
     1381 ctagcatatc tttattttta gcagcatcaa acattttaca ctcaagaaca ctatacttag
     1441 gtttttcttt atctctcatg aaataccact tgagccataa aattttttct ctgtgaatta
     1501 ctgaaatacg ttcgattttt ttctttggca tgattacctc ctaaattatt aatctaatag
     1561 ctttaaccac tcactttttg agattgttgt ataaggttca tctagcgcgt gatataaagg
     1621 ttctaaacct ttgttaaatc ggatatagtc cattttatcc tcatgctttg gaaaatataa
     1681 gattcctgtt tcatattcat ttagaccacg gttacaccaa tcatcaacga taacctgcac
     1741 ttcccattca cgccactttt taaggttttc taaaagttca atatcactaa ataactcacc
     1801 atcaacttgg aaaaattctc ccagatcatc gttaccctcg acgatgataa cttcaggcgt
     1861 atttctacta cttaataatt ccttgagttc ttgtaactct ttgatctcct ttagatactt
     1921 cctcaatttc taacctcaat tcttcaattt gtcttactac tccaaaaatt tcatgagttt
     1981 tatatgattg ttcaagtata gtttttgctg cttgagttct tataaaaggg ttaattttgg
     2041 catccatcat aatatcattg agtacagaaa cagcattgga tgaggctaga tatagcatct
     2101 gtgtagtttt atccatcatc tcatcttgtt ttagtctcaa tgtcttttta acagctgcaa
     2161 cttttagata cttatgccct gttgcccttg acacccctgc tttttggcac gctttatcta
     2221 tcgttggctc agtaagcatg gcatctatga attttatttg tttagatgta aggccatcat
     2281 tttcatttcc tgccatctat tacctcctcg ttatcaaaat aaaggggttg ctagcccctc
     2341 tatttgttta aatactcatc caaacgacgg tcaagctcag gggcatctga atttactacc
     2401 atttcagttg ttacagcttg gattttatcg ccgttgataa gcaaactgta atgacaatat
     2461 gaaagttgcc gcttaaagaa taagattaga ctttctagcg cctgctctgc attgaataat
     2521 tccaagttga tagcaatttc tggatattgt tcagcaattg accaaagttt tttaatagca
     2581 agaggtttgt tcttgtattt ttcaaaataa cctagcaatt catcctttgt catcttcata
     2641 ctagctaaca aggtcagctc tgccacgtca tcagctgtta cgctgagttc tttttctttt
     2701 aatgcctcta gctctagatc atgctgtttt tcaatttctg catgaatttt attataggtg
     2761 tccctagaat aagtgtcccc ctctaactta taagcctcta gctggtcttt agcgtatttt
     2821 tcgcctatca taccattttg taactgttta tgtaattcct cttgtttagc aatgtagcca
     2881 gtcatgtacc catgcttttt cttgccaaaa gccttaatat catccttgat tgttgtaaat
     2941 gtcatgttat cctccttacg aaaaaataaa agaggtatga caaaagagcg ttaaactcaa
     3001 ttatcatacc tctggttttt ccagtcagta tttattaaca ttgttctcca gtggtcactt
     3061 ccttataaac taactgccca tcttgagctt ttagcttaat gctaccatga tctggaattt
     3121 caatgtactt tattatacca ttttttgagt ataaaacaaa gcctttctcc atcatttcta
     3181 ataatttatt atccttgttt tcagtcatga atttctcctt tatttcaagt gtcccaacgg
     3241 gtacggttgt aagctgagac attttagggc tatctttgag cgacaaaaat gtcgaaaagt
     3301 cttcagttcc tgtataaccg ttattagttt tgtttgctac tcatttttga gtcacaaatc
     3361 tgttaagcaa aaatgagtaa tagctcgtaa gataagtaaa tctaatactt gtattcctaa
     3421 agcgttgata taactgatat tttcaactat ccaacgctct ttactaccct ttttagtgtg
     3481 ctttagctca tttttgagct tttttgagct tttttgagtt ccattttggg aatgcaaagc
     3541 aggcgcgcac cgtgcatgct tttttcttgc tcatagcctc accctcagaa gtttgccgac
     3601 cgagagcgtg gggcttttta tttttgtata attaaaaaca gctgcacaat ttttgtacag
     3661 ctacgaccga ggcaggggcg acctgccgta ctttacacta gataatatca tctaggtagt
     3721 ccaactgtat tttatcatat taaaggctac attacaattt ctcaccctgt gaagagtgtc
     3781 tatttttgtt taggtttgta agtcgattac ttcaactgct ttaccttatt ttttaactga
     3841 ttaattgagt atcgcttatc tttgattgtg aaagccttaa aagtgttacc ctcaagacct
     3901 tttagaatac ggctataatt ccgctcgttg tatactatct tgagttcatc gctatcaaga
     3961 tttgtattaa atatagtatt ttctcgatta ttcagtacat caaaaagtaa atcctgctcc
     4021 caatcgctct taggtttaat aacagcattt tttgcaccta aatcatcaat aattaagtaa
     4081 tcaacagact tcatgagttc agtagcttca aactctgtaa gtgttgcacc tttaccataa
     4141 ttccacccct ctttaatttg tttgatcatt tcggttaggc ttacaaaaag cacactctta
     4201 ggttctcctt ttgtcttata cccctcattt atacctttgg caatagcaac tgataaaagt
     4261 gtttttccaa tccctgtacc tcctgtgata agcgtatttc ccctcatgcc atcaagatat
     4321 ttttgtacct gaccttttgc aaattctaaa aatcgctttt cttctgatgt tacagcatta
     4381 aaatcatcaa aagttttagt tttaaactca tctgctacat agctcttatt gctcatcaac
     4441 acattataag tttgcagata tagtttagca ttcaaattat cagcaatcgc atcttcttca
     4501 tcttgctttt tctgttcttc ttggcattgt tcacaatagg gtgggataca gcgaacttct
     4561 tttattacct ctccgttctc attccacccc actactacat gtctttctcc tttgatttgt
     4621 gttagctgta tttcatgctt aggacacaat tcgtctagtt taaatgtctc aatatttcct
     4681 aaactagatt gtaatgattt cattttctga cctcctaaaa tggtttttct tgtgttggta
     4741 tccaatcttc atagctggta ggctctagtt gattggtttg ctgtttttta gcctcacgcg
     4801 ctgccctgct atttctaaca agttccaccg tcaataaatt gtcctgtttc caacggttaa
     4861 ggattgcctt gatgtatgca aagtttgctt taccctgact gacagcctct tttaacgcct
     4921 catggataag ctctgggcta aaatcttcta gcatatactg caattcttga atctgtaacg
     4981 gtgacaatgc tttacctgtc tcagcttcaa aagatttata gagatttaca aaatcttgat
     5041 tgatggtaga gggagtgggt gattgttctt tatctacttc tatctctaat tctttctcta
     5101 tctctgttgg acatgagttg gaaaatgtca aagatggttg gacttcttcc aattttcctt
     5161 tttttcgttg ttcacgttta taactcgccc agtttgtttc actcataacc atagcttttg
     5221 cctgtggtag ttctgcgttt ttatcttcgt caatctgtat taaaccgcat tttgtaaagt
     5281 atgccatggt catatctata tcatcttctg atacatctaa ttttagggct aactcttcct
     5341 tgagattgtt aaagtagccc tcatagtata aaatacagtc actttccaag ctctctaaca
     5401 tgagcctaat ataaataact gtcatagtgt acccaccagg taccgtcttt agtctcttga
     5461 taaaaatatt atcaaaaaat ttcttatcaa tttttagcca aaaatagact tttgtttttg
     5521 ccatcgtcct ctcccttgct catttataat tctctacctt gattagtctt aatactcgct
     5581 tgtcattcat tgttagactc attcaattct tccccatacg tctacccctg caagctggat
     5641 atatcgccca taatcagggt ttaaatcctc gctaggtgtt tctatcgtct gttggttttc
     5701 tcgctccagt tgggcgcttt ttttgcggtc tcggtggttt aaataaagca gtaagccaat
     5761 caaaatcacg gtaacaaaaa ccgcctgtgt attgcttata tctagttcat tcatgttatg
     5821 ccctcgcttt gtaattcttg atgtatgcca cttgttcagc tcgcttcata ttcaacaagt
     5881 cgtctatttc cacactggtt acttttttat ttacaaaatc agaaatcagt tgaaaaatgt
     5941 ttggactttg tagctggatt tcagccattg cctcatcaaa ttctgcttgt gtcatgttgt
     6001 ctaaatctag tgtcattgca ttgcctcctc aaacttctct ataagacaac ttttatttgc
     6061 tttctgagtt ccatttttag agttaaaaag aatatatttt aaggttacag tagcctctaa
     6121 atactccttt tcagcatgct ctatatacgc ctgttgctct gcttcgttct caaaaaagtg
     6181 cttagcttag cgtttaaaga atgctttgac taggtttagt ttgaattgcc gtactgcttc
     6241 ggtattcttt agataagtga tcagcaaagt agcctgttgc tcgttcagaa tataggattt
     6301 tttaggttgt cctctagtgt ctaatttatg gattttaaat ccaagtattc ccaactcttc
     6361 aaagtcaacc ttattttctc ttattaagcg tgtgatagtg tggtgttgta cgtcagcaca
     6421 ttcagcgatg atctcgcttg tggtatacgg ctctttcttg ccgtccatgt agactagttc
     6481 cattatggtt ctacctcctg tataaattta gctagcttac tttttaattg tctcctctag
     6541 ccctttttag cctctacaac ggttttggct aacgtgttag aggcttttat aacggcctgt
     6601 atagctgtct ctatttcctc cgtggaatag tagctttgtt ttctaatatc atttaagact
     6661 tgtttaaccc atttcttgaa ataaataaaa ttacatcttc tgtactcttg gcatctgctt
     6721 tatctgagac aaatagaatg tcaatgcctg gtagataaaa cccaccgtct atttgtttag
     6781 ttggtttgaa agtaactatt ttcatttcat aactcctatt ttattgctaa aaataaatac
     6841 acatctgaca gacgatagaa tactttctta gctccatcca tcggggtctg atatcgttta
     6901 agagcaagat cctcccagtt atcaagggtt gtagcactta tacctagcag atccattaat
     6961 tctttacgag aaattaaatc taacttacta gatcgttgtt tttctagctc aactaaagtt
     7021 tcaagagtct gtttaagaag ctgtaaaccc tgtaactgta tttctttaga cagaatttcc
     7081 atttatatcc tccattttcc tttttataaa ttaagttgtc tttttcctct aattttgata
     7141 aaataagaag agattattta gcaaaggggg ttgtcccttt gctaaatacc taacaaaacc
     7201 acgcgccttg cctttgcttg agttttgtta tttttcctta gccgtgtacg ttggtttggt
     7261 cgcctgtaca tggcttttta cttgcgttaa atttattcta ctagccaagt catcacgctc
     7321 tcatagatac gcttagaggc atcatagttt ccattctcaa cttttgttag tgtttgagat
     7381 ttaataccca actgctccga tactgctttt ttggttagct gtaatcttgc acgcttgata
     7441 cgtacttttt cagcaagtga tagtgtaatc aacattggtt tcacctccct tttttctcca
     7501 tttttggcat tttgtgttat aatctcttta gaaaggatgt ataagatgaa tagaattaag
     7561 caattacgaa aagaaaataa tcttactcaa agagaacttg ctaatgagac taaaatccct
     7621 tatagaacca tacaacgttg ggagaatggg gaaactgata taaaatccga tgctgctcag
     7681 gtacttgctg actattttgg agtatcaatt cctcacttac taggttatag cgatttgata
     7741 gcagatacag atagtgatat taaagcaatg gcatactcac atctactaac ttttgtagat
     7801 gaaaaacaaa tcaaagaatt tgaaaaagag ttcattcgtg caaacgataa tagtgatact
     7861 tttggtgatt taggaagtgg tgaaaaggcg ttagaaaaat actatcaagc attatgctat
     7921 ttagttcctc cttttaggga tatattggct aggtatagct ttttgtcact agatgaaaaa
     7981 aaatcagtct ataatattat tgttggttta agtaacaact tatcaaaaga aaataaataa
     8041 ctttaaacat catgtataaa tacctaacaa aaccacgcgc cttgcctgct gatggaaaga
     8101 aaggtacaaa tacatgaata tcatagagaa aattaaaaag aatggccaaa gagtttatta
     8161 tgctagtgtt tatctaggcg ttgaccaact aacgggcaaa aaagcccgta caactgttac
     8221 agcaaccact aaaaagggcg ttaaagtaaa agcgcgtgat gcgatcaata cttttgctgc
     8281 taatggctat acagttaaag ataagccgac aattacaaca tacaatgagc ttgtaaaagt
     8341 ttggtgggat agttacaaga atacagttaa gccaaatact cgccaatcca tggatggatt
     8401 ggttagagtg catttattgc ctgtatttgg cgattacaag ctatctaaac ttactacgcc
     8461 tattcttcaa cagcaagtaa acaaatgggc tgacaaggca aataaaggcg aaaaaggggc
     8521 atttgctaac tactccttgc tccataacat gaataagcgt attttgaaat atggcgtagc
     8581 tatccaggta atacaataca acccagctaa tgatgtcatc gttccacgca aacagcagaa
     8641 agaaaaggct gctgtcaaat acttagacaa caaagaatta aaacagtttc ttgattattt
     8701 agacactctg aatcaatcaa attatgagaa cttatttgat gttgtcctgt ataagacttt
     8761 attggccact ggttgccgta ttagtgaggc tctagctctt gaatggtctg atattgacct
     8821 agaaagcggt gttatcagca tcaataagac actaaaccgc tatcaggaaa taaattcacc
     8881 taaatcaagc gctggttatc gtgatatacc aatagacaaa gccacattac ttttactgaa
     8941 acaatacaaa aaccgtcaac aaattcagtc ttggaaatta ggccgatctg aaacagttgt
     9001 attctctgta tttacggaga aatatgctta tgcttgtaac ttacgcaaac gcctaaataa
     9061 gcattttgat gctgctggag taactaacgt atcatttcat ggtttccgcc atacacatac
     9121 tactatgatg ctctatgctc aggctagccc gaaagatgtt cagtatagat taggccactc
     9181 taatttaatg atcactgaaa atacttactg gcatactaac caagagaatg caaaaaaagc
     9241 cgtctcaaat tatgaaacag ctatcagcaa tttataaaaa ataagggtga cccatttccg
     9301 ggctaccctc ttactatacc aaaaattagt aggggtagta aaaagggtat taaattataa
     9361 aaagcactaa gggaaagcgc cccaaagtgc ttatttcaaa ggctttatag cctataatca
     9421 cataaggaga ttatttttta aggttgtaga atgatttcaa tccacgatat tcagctactt
     9481 caccaagttg gtcttcgata cgaagcaatt ggttgtattt agcgatgcgg tctgtacgtg
     9541 aaagtgaacc agtcttgatt tgtcctgcgt tagttgcaac tgcaatatca gcgattgttg
     9601 aatcttcagt ttcacctgaa cggtgtgata caacagcagt gtaaccagct tctttagcca
     9661 tttcgatagc ttcaaaagtt tcagtaagag taccgatttg gttaactttg ataaggattg
     9721 agttagcagc accttcttgg ataccacgtg caaggtagtc agtgtttgtt acgaagaagt
     9781 cgtcaccaac aagttgtact ttcttaccaa gacgttcagt aagagctttc caaccatccc
     9841 agtcgttttc atccatacca tcttcaatag tgatgattgg gtatttgtta accaattctt
     9901 caaggtagtc gatttgttct gcagatgtac gaacagcagc accttcacct tcaaatttag
     9961 tgtagtcgta aactttacgt tctttatcgt agaattctga tgaagcacag tcaaatccga
    10021 taaatacgtc tttacctggt acatatccag cagcttcaat cgcagcaagg atagtttcaa
    10081 caccatcttc agttccttcg aaacgaggag cgaatccacc ttcgtcacct acggcagttt
    10141 ccaaaccacg tgatttaagg attttcttaa gagcgtggaa gatttcagca ccgtaacgaa
    10201 gggcttcttt aaatgttggc gcaccaactg gcaagatcat gaactcttgg aaagcgattg
    10261 gagcgtcaga gtgagaacca ccgttgatga tgttcatcat tggagttgga agaactttag
    10321 tgttgaatcc accaaggtag ctgtaaagtg ggatttcaag gtagtcagca gcagcacgag
    10381 ctacagcgat agacacaccg aggattgcat tcgcacccaa tttacctttg ttaggagtac
    10441 cgtcaagtgc gatcatagca cggtcaatag cttgttgatc acgtacatcg tagccaatga
    10501 tagcttcagc aatgatgttg tttacgttgt caacagcttt ttgtgtacca agaccaccgt
    10561 aacgagattt gtcaccgtcg cgaagttcaa ctgcttcgtg ttcaccagta gaagctcctg
    10621 atggaaccat accacgtccg aaagcacctg attcagtgta aacttctact tcaagtgttg
    10681 ggttaccgcg tgagtctagg acttcgcgag cgtaaacatc agtaataatt gacatttttt
    10741 actctcctta tgagttaaat tttttacacc tctataatac cttaaaaccc ctcctttttc
    10801 aagaaaaaac gttatctttg tgcaactttt ccttaacttt ataaagtaat cgctttcttt
    10861 tgtctgtttt attctaactt ttatgatata ctgttttcat gacagattta tcaaaacaat
    10921 tacttgaaaa agctcatggt gggttaaaaa taaatccgga tgagcaaaga cgctatcttg
    10981 gtacttttga ggaaagagtt cttggatatg tagatattga cacagcaaat agccctcagt
    11041 tagaaaaagg ctttttattt attttagaaa accttcagga aaaagcagag ccactatttg
    11101 tgaagatttc accaactatc gaatttgata agcaagtttt ctacttaaaa gaagcaaaag
    11161 aaactgatag tcaagccacc atagtatctg aagagcatat tacttctcct tttggcctgg
    11221 ttattcatag caatgcacca gttcaagtag aagaaaaaga ccttcgactt gcttttccaa
    11281 aactttggga agttaaaaag gaagaaccag ccaaaacatc cttatggaag aaatggttta
    11341 gctaaatctt gcacatattt aataagtgcc caatattggc agccgtgcgc tccagataga
    11401 gactggcatt tttcaaacta tcttctaaag gttcactttt ctccaaaata gaaaagacag
    11461 cttggatatt ttcaaatggt aggggaggta aatcttcagc aagactaccg caaatagcaa
    11521 taacaggaac tccaacaggg gttctttttg caacacctat aggcgctttc ccagcaaagc
    11581 tttgactatc aagtcttcct tctccaacaa caaccaagtc agcatctgaa actttcttat
    11641 caaagttgat taagtccaag caggtatcaa ttccagacac gatacttgcc tgagcaaaag
    11701 cacacaaacc accagcaagg cctccacctg ctcctgctcc tttaatttct aatgttgcag
    11761 gtgagaattt ttcataaaaa tcttggatcg cctgatctac gactgcaaac atagtcggat
    11821 gtcgaccttt ttgattgcca aaagtataag tcgcaccttg atgaccacat aagggactta
    11881 cgacatctgc taaaatatga atttgaacac cttcaggaat tttatagcaa ttttctgttg
    11941 aaacagaagc taagtttaat aaggattgac cggaagcagg caagacattt ccatccctat
    12001 cataaaattg ataacctaaa ccagcagcaa tccccagtcc tccatcatta ctggccgtgc
    12061 caccaacatc gatataaata tctttaatcc ctttagaaat gagatggaga atcaactctc
    12121 caataccaca agtttggatt tgaagtggat ttcgtttctc tagcggaatt tttccaagac
    12181 caaccaagtc agctacttca aatagtgcca gttccccttt ttgaaaatag cgcatggctt
    12241 ctttttgtcc aaaagggtct gtcacttgga tccatttttc ttttaggtca agagaatgtc
    12301 ggatagcatc tacagtacct tctcccccat caccaacagg gcagaggaga cattctacat
    12361 ctgctatcga ttgttggaag cctcttttta ttgcttcagc tacctgttga gctgtcaagc
    12421 tttccttaaa cgaatccggt gcaattacaa tcttcatatt ttccctcatt ctaaacagtc
    12481 aatcaaagga agaacttcta aaaaatccct cttgtcaaca tgatgtggta tttctttttt
    12541 gagcatttct ttggaacaaa aggcgattcc taactccgcc gacttcaaca ttaatagatt
    12601 attgccccca tcaccgattg ccaccgttct ttctttagaa agttttagtt tctttctcca
    12661 tttttccaga gtttcttttt tgacctgggg acttataatt tgtccaacta attttcctgt
    12721 taaaagacct tctttgactt caagctggtt agcagtgaaa taggcaatac caagggattt
    12781 tgctaatctc tccaactatt ggtgtaaatc caccagacac cagaccaact aggataccat
    12841 tcttttggag aatagagatg aactctggga catttagcga tagatgaatt gagttgaaga
    12901 cgttatcaaa gaccaaaata ggaagacctt ccaacaagga cactcttttt cttaaactgc
    12961 tttcaaagac caactctcct cgcattgctc gacttgtaat ctgcgaaatt tctgcctcat
    13021 gacctgcctc tctccctaaa agatcaatca cttcttctag gattaaggtt ccatctacat
    13081 ccaaaacaca caagcctttt acttgagaca tcagttctcc tctctaaaca gcctaaaaat
    13141 cgtatgaagt catcatacga ttttatctat taattaacta aactatggta caagtcaagg
    13201 tatgacttgc aggctgtatc ccatgagaag tcactctcca tagcttgttt ttgtaggttt
    13261 ctccaaatgt ctggatggtt tctatacaag tccaatgctg tttggaaagt ccaatttaac
    13321 caataaggag atagattgtc aaagctaaag ccagtaccgc ttccttcgat tggattgaaa
    13381 gcgcgaactg tatctcgcaa gcctccaact tcatggacca atggcaaggt tccataacgc
    13441 atagccatca tttgagacaa gccacacggt tcaaaacgac ttggcatgag gaagaggtca
    13501 caagcagcgt agatttcctg agcaagtttg acatcaaaag tgatatttgt tgatagcttg
    13561 tctgggtaaa tctgagcaaa ccatgagaaa gctccttcaa aggctggatc gccagttccc
    13621 aaaagaacaa tctgaacatc ttcttgcaag atatggtgaa gactttcgac caccacatca
    13681 aaaccttttt gacgtgtcaa acgagaaaca attcccacca gtggaacgtc tgctctaaca
    13741 ggcaagccaa ctctttcttg caattttgcc ttatttttgg ctttcccaga caaatcttcc
    13801 tgattgaaat gatagtctaa aagagcatcc gtctgaggat tataaagatc agcatcaatc
    13861 ccattcacga taccagatac tttaccagac tccattttaa gaatctgatc caaattacat
    13921 ccaaactgac tagtcataat ttcatgagca tagctaggtg aaacggttga aacacggttc
    13981 gcatagagaa tacctgcctt catccagttc agacagttgt tccatcgaag ggtgccatca
    14041 gcgtaacgtt caaagccaac tccaaacaaa tcacccaaca ttccttctga aaattgtcct
    14101 tggaattcta aattatgaat ggttaaaact gtttcaatgt cctcataggc ttgaatccaa
    14161 cggtattttt ccttcaacaa gaaaggaatc atagctgtat ggtagtcatg aacatggaga
    14221 agatcaggaa taaagtcaat cctttccata gcctcaatgg cagccagttg gaaaaaggca
    14281 aagcgttctc cgtcatcaaa atcaccgtaa acatgaccac ggaagaaata atattgattg
    14341 tcaataaagt agaaggttac accatttaat actgttttct taattccaca atactgtctg
    14401 cgccaaccaa cgctcacctc aaaatgaagc acatcttcaa tctgatttcc aaatttagcc
    14461 tctaccatat catagtaggg taaaatcact gcaacttcgt gcccagcttt taccagtgat
    14521 tttggaagag cgccaatgac gtctcccaaa ccacctgttt ttgaaaaggg tgcaccctct
    14581 gctgctacaa ataaaatttt catgaatgaa tatcctctgt tactttagca cctttcttaa
    14641 ccacaactgg atgttctgca gttcctcgaa tcacaacacc aggctcaact tcaacccctt
    14701 tgtccaagat agcatattcg acctgagccc cttctccaat aacaacacga gggaagagca
    14761 ggctatcttt aaccaagcta tccttatgga catggatatt acgtgataga acagaattag
    14821 ctacttgacc ttcaataata ctaccagagg caaactgaga agttcttacc ttagatgtat
    14881 tagcatagta agttggctct tcgtttttga cctttgtata aatcttttgg tttggtgaga
    14941 aaagagaata gaatttttgt gattcaagca tatcgatatt cgcttgataa taagatttaa
    15001 cagagtgaat attggctaga tagcccgtgt actcgtaggc gaaagctccc tcttttacag
    15061 ccaaatcccg taaaacatag cgcaatttct ctggatgttc ttttttagct tcttcttcca
    15121 agtgttcaat caaccaaggt gtatcaacga caaagatatc tgtagacata ttgaacgttt
    15181 cagctgttga cttgctatca aagagtttat gagaaagaac atggtctgtt tcatctacat
    15241 ccaagattgc atttacttct gaaatatctt tcttagctag ttttttataa actacagtga
    15301 taggctcttt tgttgtacta tgtaggtgga aaacttggtt caaatcaatg ttaataagaa
    15361 catcgcagtt gagggcaacc gtttggtttg agccagaacg tttcaaataa gtaagaagct
    15421 gttggtagta ttcttttcca actgtactac tttctacacg ggtattgtaa attcctagat
    15481 agtaatggct aagaaaggtt gataagcccc actcgcgtcc tgaacgaata tggtcaaata
    15541 ctgagctgat attatcctgc tggaaaatac caaagacact acgaacacct gcattagcaa
    15601 ggcttgaaag tgggaagtca atcaaacgat atttcccacc aaatggcaaa cttgctactg
    15661 gacggtggtc cgtcaatgtc gacatattgt gaaaaccaac tgtatttcct aaaatggcag
    15721 aatatttatc aatcttcatc tgttgctacc cccactactt cattatatcc tacaacttgt
    15781 acttcatctg ttccatcaat ttcgacaccg tcagaaataa tcgcaccttc accaataatg
    15841 gcacgtttaa tcttagctcc ttgaccaatg atagctccac tcatgataac tgaatcaagg
    15901 acttccgctc cttcgcgaac ttgcgcgcct gttgaaagga tagaatgttt aacagttcca
    15961 tcaacgaaac atccgtctac aactaatgag tcttccacat gagcatttgc cccgaggaag
    16021 tttggtggtg aaatcaagtt tcttgagtaa atcttccatt gacggttacg actatccaag
    16081 gcattttctg gagaaatata ctccatgttc gcttcccaaa gtgactcaat agtaccaaca
    16141 tctttccaat aaccactaaa ttcgtaagca taaacacttt cacctgactc aaggtaattt
    16201 ggaatgacat ttttaccaaa gtctgacatg ccaaccttgc tcttttcagc agcgactaac
    16261 atattacgaa ggcgttgcca atcaaaaatg tagattccca tagaagcttt tgtagattta
    16321 ggttgagctg gtttttcttc aaattcaaca atacgattgt tagcatctgt gttcatgata
    16381 ccaaaacggc ttgcttcttt aagagggacg tctaaaactg ctactgtcaa gctggcatta
    16441 ttatccttat gagactggag catatcatca tagtccattt tgtagatgtg atccccagac
    16501 aaaatcaaga catactcagg attgacactg tcgatatagt cgatattttg gtaaatagcg
    16561 tgactagtcc cctcaaacca acgatttcct tcacttgcag aataaggttg aagaatagag
    16621 acacctgaat taataccgtc tagtccccag cttgaaccat tcccaatatg gttgttgaga
    16681 gcaagtggtt gatactgtgt aacgacccca acattatgaa tccctgagtt ggcacagttt
    16741 gatagggcaa agtcaatgat acggtagcgc ccaccaaatt gcacagctgg ttttgcgatg
    16801 ctttgagtga gtttaccgag acgagttcct tgcccaccag caagaatcaa agctaacatt
    16861 tcatttttca ttttctactc ctttttggtt tttatttgtg acggttttag tagatttcaa
    16921 gcgacgtttg attttccata cacttgctcc catagccggt agggtaaagg ttaaggtctg
    16981 ctcataatct ttccatagtc cttcttgcgt ttgaacagtt tgattatgtt ctttccaaac
    17041 gcctccccac tcttccaact cagtattcca tacttcttcg taaattcctg caacgggtag
    17101 tccgattgta aaatctttcc gctcaacagg taccatgtta aagatacaga ctaacatttc
    17161 tcccttttta cccttacgaa taaaggaaag aacactctgg tctcgattat ccgcatcaat
    17221 gacttcaata ccatcatagc tggtatcgat ttcccacaga cagcgatgat ctttgtaaaa
    17281 ctggtttagc tgagaagtga aatacttcat cttagcattc attgggtctt ctaggttaga
    17341 ccattccaac tgttcttcag atttccattc taggaattga ccgtattcgc tacccatgaa
    17401 gagcaatttc ttaccagggt gacaaatttg gtacgtatag agattgcgca agcctgcgaa
    17461 ttgattgtaa cgatctcccc acatcttatg catcatactc ttcttgccat gaaccacttc
    17521 atcgtgcgag aatggcaaga gataattctc cttgaaaaca tacataaagc tgaaagtcac
    17581 caggttaaag tcatatttac gataaatcgg atcttcttcg tagaaacgga ggatatcatt
    17641 catccagccc atgttccatt tgtagtcaaa tcctagacca ccaatctctt tcattcccgt
    17701 aatcttggtt gcagacgaac tttcttctgc aatcatcatc acatctggat attctaactt
    17761 aataacttca ttcaagcgtt gaaggaaata ataaccttca tagttgagat ttccgccatc
    17821 tttattaggt gtccatggag catcatcata gtccaaatag agcatgttgc taacagcatc
    17881 cacacgaata ccatccaaat gatagatatc aatccaatgc ttaatgcaag aaattaagaa
    17941 ggactggact tcattttttc caaggtcaaa attaagggca ccccaaccat ggttatgagc
    18001 cttattatgg tcttggtatt caaaagtcgg tgtcccatca taataggcta aggcatcatc
    18061 attgatggta aagtgacctg gtacccagtc cacaataacc ccaatattat gggtatgaca
    18121 ctcctcgaca aaatcttgaa actcctctgg tctaccatag gcatgctcta aagcgaagta
    18181 acccataagc tggtaccccc aactcaaacc tagtgggtgg gacatcaagg gcataaactc
    18241 aatatgagta tagttcatct caactaggta aggaatgagt tcatctttca attgggcaaa
    18301 actataagga ctgccatcag gatttctctt ccaagagcca gcatgaacct cgtaaatatt
    18361 gacaggacgt tcagcaaagc cccaacgttt tcttcgtgcc agccaaagtc catccctcca
    18421 tttcttatca ggaagctctg ttacgattgc ccctgttcct ggacgagcct catacctgac
    18481 agcaaaaggg tcaatcttca tcagttgatg accattttga cgtgtgacat gatatttgta
    18541 aatatgccct tcttgagcca tattggtaaa gacttcccag accccaaagt catttcttac
    18601 cattggaatc tgattttcaa tccaattggt aaaatcacca accaagtgaa cagcctgagc
    18661 attaggtgcc caaacacgga aggtatagcc atactctcca tttagttctt ccctatgtgc
    18721 tcctagataa tgttggagat aaaaattttc acccgtcata aaggttttta atgcttctct
    18781 attatccata tactcccttt ctcctgtaag cgttttctat gtttttatta tactaccttt
    18841 ttagagaaga ttcaagtaaa ttactatact tctttaatta ttttgaaaat ctacaacaag
    18901 ttcacttact cgttcaattg taaatcaata ttttttcaaa aaattgcgaa aacacctttc
    18961 tttttctact atagtgaaat gaaataaaac atgcgcaaat cgattaagga atttaatcta
    19021 atttctaaca atgtcttaga aatcaaagtg t
//
LOCUS       DAVBCB010000046        17639 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVBCB010000046 DAVBCB010000000
VERSION     DAVBCB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17639
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.046"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..224)
                     /locus_tag="VQU28_001556"
     CDS             complement(<1..224)
                     /locus_tag="VQU28_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HET2476889.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCT"
     gene            262..1038
                     /gene="recX"
                     /locus_tag="VQU28_001557"
     CDS             262..1038
                     /gene="recX"
                     /locus_tag="VQU28_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HET2476890.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYKLKQRLTQVLARKGYDFSDIASALREYL"
     gene            1127..1660
                     /locus_tag="VQU28_001558"
     CDS             1127..1660
                     /locus_tag="VQU28_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HET2476891.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(2100..2856)
                     /locus_tag="VQU28_001559"
                     /pseudo
     CDS             complement(2100..2856)
                     /locus_tag="VQU28_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(2994..4616)
                     /gene="groL"
                     /locus_tag="VQU28_001560"
     CDS             complement(2994..4616)
                     /gene="groL"
                     /locus_tag="VQU28_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HET2476892.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(4632..4916)
                     /gene="groES"
                     /locus_tag="VQU28_001561"
     CDS             complement(4632..4916)
                     /gene="groES"
                     /locus_tag="VQU28_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HET2476893.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(5186..5581)
                     /locus_tag="VQU28_001562"
     CDS             complement(5186..5581)
                     /locus_tag="VQU28_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HET2476894.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(5659..6420)
                     /locus_tag="VQU28_001563"
     CDS             complement(5659..6420)
                     /locus_tag="VQU28_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HET2476895.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFPLDVTDMVMVKTVCSDILETIGRIDILVNNAGLALDLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMIKVNQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(6453..7079)
                     /locus_tag="VQU28_001564"
     CDS             complement(6453..7079)
                     /locus_tag="VQU28_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HET2476896.1"
                     /translation="MIFTYNKEHVSDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(7095..7412)
                     /locus_tag="VQU28_001565"
     CDS             complement(7095..7412)
                     /locus_tag="VQU28_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HET2476897.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(7409..7708)
                     /locus_tag="VQU28_001566"
     CDS             complement(7409..7708)
                     /locus_tag="VQU28_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HET2476898.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(7993..8184)
                     /locus_tag="VQU28_001567"
     CDS             complement(7993..8184)
                     /locus_tag="VQU28_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HET2476899.1"
                     /translation="MYYSVDDVVSNAFKKRMILDSFFAFNCSGTMKVSTWVYDKGEWY
                     YVSSSGSMIANDWVKDNGK"
     gene            complement(8345..9019)
                     /locus_tag="VQU28_001568"
     CDS             complement(8345..9019)
                     /locus_tag="VQU28_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476900.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLTIRGDVAFGKL
                     EIVYVK"
     gene            complement(9025..9471)
                     /locus_tag="VQU28_001569"
     CDS             complement(9025..9471)
                     /locus_tag="VQU28_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2476901.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(9573..10088)
                     /locus_tag="VQU28_001570"
     CDS             complement(9573..10088)
                     /locus_tag="VQU28_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HET2476902.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVCVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLFLHVS"
     gene            complement(10085..10447)
                     /locus_tag="VQU28_001571"
     CDS             complement(10085..10447)
                     /locus_tag="VQU28_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 family protein"
                     /protein_id="HET2476903.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICSVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(10513..12372)
                     /locus_tag="VQU28_001572"
                     /pseudo
     CDS             complement(10513..12372)
                     /locus_tag="VQU28_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001850459.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            complement(12389..12859)
                     /locus_tag="VQU28_001573"
     CDS             complement(12389..12859)
                     /locus_tag="VQU28_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HET2476904.1"
                     /translation="MDKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVHFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNGLVELEASPVDKRAKFVRLTDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQANIKGIGGENKEISQKN"
     gene            complement(12983..13699)
                     /locus_tag="VQU28_001574"
     CDS             complement(12983..13699)
                     /locus_tag="VQU28_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HET2476905.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(14580..15989)
                     /gene="ply"
                     /locus_tag="VQU28_001575"
     CDS             complement(14580..15989)
                     /gene="ply"
                     /locus_tag="VQU28_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HET2476906.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQHEKITAHSMEQ
                     LKVKFGSDFEKIGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLRQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFESLIKGVAPQTEW
                     KQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTSFL
                     RDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWDELSYDHQGKEVLTP
                     KAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRKRT
                     ISIWGTTLYPQVEDKVEND"
     gene            complement(16000..16332)
                     /locus_tag="VQU28_001576"
     CDS             complement(16000..16332)
                     /locus_tag="VQU28_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476907.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDYLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            complement(16403..17011)
                     /locus_tag="VQU28_001577"
     CDS             complement(16403..17011)
                     /locus_tag="VQU28_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476908.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(17024..17482)
                     /locus_tag="VQU28_001578"
     CDS             complement(17024..17482)
                     /locus_tag="VQU28_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HET2476909.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
BASE COUNT         5206 a         3929 c         3221 g         5283 t
ORIGIN      
        1 atagtacaag atggcacaat tcgaaattta gacttcttgt tgaccttcag taattttgct
       61 tcaacaaagt tgcgtctaat agaagtaatc tgacaataga tatcttcgcc tttgagagct
      121 cctggtacaa agactaatgt tttttggtaa aagccgattc cctcaccgtt aattcccatg
      181 cgcttgattt ttaatggtat tttttgtttc actttcagat tcatacccct atcttatcac
      241 attttgagtt ataatagagc tatgaaaatc acaaaacttg aaaagaaaaa aagactctat
      301 ctgatggagc ttgataatgg cgacaaatgc tatatcaccg aagatacaat tgttcgtttt
      361 atgttatcga gagataaggt gataagcgaa gaggaattga aagagattca agactttgct
      421 caattttctt atggtaagaa tctggccctc taccacttat cctttaaagc acgcactgaa
      481 aaagaagtca gagaatatct gaaaaaatac gatattgata aaaacatcgt ttctcaagtt
      541 attgctaatc ttaaagaaga taagtggatt aatgatggtc agtacgctta tgctatcatc
      601 aatgccaatc aactttcagg agacaaggga ccttatgtac tgactcagaa attagcacaa
      661 aaaggaattt caaaatctac tatagaagaa atcttgaatg attttgattt ttcggaagtt
      721 gctcaacgtg tagccaataa actattgaaa aaatatgagg gaaaacttcc agctcgtgcc
      781 ctgcaagata agattatcca gaacttgact aacaagggct tctcttattt tgatgctaaa
      841 attgcctttg acgaattgga tagtcaagtt gatgaagaaa cgactcagga actcatcttc
      901 aaggaacttg ataagcaata tactaagtat gcccgaaagt atgaaggata caaactgaag
      961 cagcgtttaa ctcaagtttt agcacgaaag ggctacgatt tttcggatat agcaagcgct
     1021 cttagagaat atctttaata tttccaagtg aaattcacag attttaggta attttatggt
     1081 acaatagtaa atgataaact tataaattgt agaaagttgg ttagttatga agcttccaaa
     1141 agaaggcgac tttattacaa ttcaaagtta taagcatgat gggagtctcc accgaacttg
     1201 gcgggacacc atggtactaa aaacaacaga aaacgccatt attggtgtca acgatcatac
     1261 acttgttacc gaaagtgatg gtcgtcgttg ggtcactcga gaaccggcta ttgtttactt
     1321 tcacaagaaa tattggttta atatcattgc catgattcgt gataatggaa cttcttacta
     1381 ttgcaatatg gctagcccct actatctgga tgaagaagca ctgaagtata ttgattacga
     1441 tttggatgtt aaaattttta cagatgggga aaaacgtctc ttggacgttg aagagtacga
     1501 gcgtcacaaa cgcaaaatga attattctga tgacttggac tatattttaa aagaacatgt
     1561 caaaattctt gttgattgga ttaacaatgg acgtggtcct ttctcagaag cctatgtaaa
     1621 catttggtac aagcgctatg tagaactaaa gaatcggtaa agttgtcaaa ggtcaggaac
     1681 aaaatcctga ccttgttttt ttggtagtat aaaataaatt gtgtaaacac aaaaaggaat
     1741 aaatccatta tagcagagtt acaacacatt tctataaaga aatttattcc tacgactcag
     1801 tttggcatgc ttaacttcct aactcaaaaa caaagcatcg attaatttat ccgttcttct
     1861 tttgaaacag atttgaactg ataattgctc gttttaattt aagaagctct tttgagcaaa
     1921 acttcatttt tttaagagtc caacaatttc tgtctatcgc ttttctacta gcaaaaaacc
     1981 tcccttgagg caagttttca tttatatatc taatttcgta acagtatagt aaaatgaaat
     2041 aaggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtgtact attctagttt
     2101 caatctacta taaatttgat atgagaatag tctttctcaa catccttatc caacaagcat
     2161 gctgtggcgt ccttcttaac ttgaaccagg tcaatattct gggcttgggc tgccatagac
     2221 gcagccacaa taacattgac gatagatatc atactcctca cacatctcta ctgaacgttt
     2281 gtagccttga tttttcttga aatcgttggg aagatagtgg gtcgtgtaaa ttttttgcac
     2341 atcgattccg atgctattga tggtttgaga attcttatga ggactgatgg tcaaggctga
     2401 accaaagtag tcaaagccca agtccatagc cacttgcgct gttttatcca gtcggtagtc
     2461 aaaacaaacc ttgcaacggt cgccaccttc gggctcctcc tctagtcccc taactagttt
     2521 tcggtattaa ttgggttcgt agggagcttc taggtactga accgtatttc ctgtccgatc
     2581 attaaaatca ctaacaaatt tcttgatgac atagacccgc ttatggtatt ctactttggg
     2641 atggatatta gaattggcaa aatagatggt cacatctgca tacttggtca aatattctag
     2701 tgtataggta ctacaagggg cacagcaaac atgcacgaga atggttggcc gctgctcatt
     2761 tttctcccat gcttgtacca ttttctgcat gacacggtca taattaatct tctgattggg
     2821 gggcatcttg cacagaattt cttctacatc gatcatgttc ttctcctttt tctagtctta
     2881 ttttatcata taagttagaa gagctcaaat caatttagaa gtgaatatta taaaaagaag
     2941 ggttagtcat tccctccttt tgtgtttttt ataagttgtt ttctatagaa agcttacatc
     3001 atcccgccca tcatacttgg atccattgct ggagctgggg ctactggttc tggtttattg
     3061 gctacgactg cttctgttgt caaaatcaag ctggctacag atgctgcatt ttgtagggct
     3121 gaacgactca ctttaactgg atcaatgata ccttgatcaa tcatgttaac ccactcgcca
     3181 gttgctgcgt tgaatcctat accaagctca gcatttttca aacgatcgat aacgatagat
     3241 ccttcaaatc ctgcattgtg agcaatttga cgaacgggtt cttccaaagc acggagaaca
     3301 atattacgtc ctgttgcttc atctcctgtc aattccaagg tagcaacagc tggaatcaca
     3361 ttggcaagag ctgttccacc acctgcaaca ataccttctt caacagctgc acgagtagcg
     3421 ttgagggcat cttcaatgcg gagtttcatt tctttcaact cagtttcagt tgcggctccg
     3481 accttaataa ccgctacacc acctgacaat ttggccaagc gttcttgcaa tttttcacgg
     3541 tcaaattcag aagttgtagt ttcgatttga gacttgataa ccgcaacacg gtgagaaatc
     3601 gcttcaggat ttcctgcacc ttctacaata accgtgctat ctttgtccac ggtcactctc
     3661 gctgcttgac caagagcttc aattgtcgca tctttcaact caagaccaag gtcttctgtg
     3721 ataactgttc cgcctgttaa gatggcgata tcttcaagca tggctttgcg acggtcacca
     3781 aaaccaggtg ccttgactgc tactacgttg aaggttccac gaatcttgtt caaaacaaga
     3841 gttggaagag cctcgccatc cacatcatcc gcaataatca agagtggacg attgctttgg
     3901 agaatgcttt ccaaaagtgg caagatttct tggatattgg aaattttctt gtctgtaatc
     3961 aaaatgtacg gattttcaag gtcagccacc attttttcgc tatcagtcac catgtactgt
     4021 gaaaggtaac cacggtcaaa ctgcattcct tctacgactt caagctctgt ttccatacca
     4081 cgtgactctt cgatggtgat gacaccgtct ttgccaactt tttccattgc ttcagagatg
     4141 tactcaccaa ctttttcaga acgagaagat acggctgcaa cttgagcgat agcttcttta
     4201 ttggcaacag ggatggcgtt gtttttcaaa gcttcaactg ctgcggcaac tgctgtttca
     4261 atcccacgac gaataccgat tggatttgca cctgctgtga cgtttttgat tccttcacgg
     4321 acgattgctt gggtcaaaac agttgcagtt gtagttccat cacctgcgat atcattggtt
     4381 tttgaagcca cttctgatac caatttggca cccatatttt caaaatggtc ttctaattca
     4441 atttctttgg caatagtcac accgtcattg gtaatcaatg gtgagccaaa tgatttttca
     4501 agaacgacat tgcgaccttt tggtcccaag gttactttaa cagtgtctgc aaggatatcg
     4561 acaccacgga ccatagctga acgggcatct gatgaaaatt taatttcttt tgacatactt
     4621 actttctcct tctattcttc aatgattgcc aagatgttag cttcgcctac gatgatgtac
     4681 ttttcatcgc catctttgac atcaagacct gcgtgggctt caactaagac acggtctcca
     4741 gttttaacac ttggagcaac caagtcaccg ttcaaggtac gaacaccttg tccagtagcc
     4801 acaacttggg ctgttttggt tttttcttgg gctgaacctg cgagaacaaa gcccccaacg
     4861 gtttgttctt tttcttctat ttttaagacc acacggtccc ctaatggttt caacatctga
     4921 tttcctccat aatgagatag atattattag cgctctttat acctgagtgc taattcatag
     4981 ttctattgta tcacttagtc agaaataatc aagaaaaaag tctgactttc tcaagataaa
     5041 aagcctgaga ccaactcaga ctttttaatg tttatactca atgaaaatca aagagcaaac
     5101 taggaagcta gccgcaagct gctcaaagca ttgctttgat gttgtggata aagctaacgt
     5161 ggtttgaaga gattttcgaa gagtattaaa atggcaattc ttcctcttcc aagactaaat
     5221 ctgccaaatc ttggcctgca ttattttcac gcatggcacg ttgagcacga ctttccaaga
     5281 gttggaatcc tgttacaagt acttcggtca cgtagttcat ttggccattt ttctcaaagc
     5341 gacgggtacg caattctcca tcaacggaaa tgagactacc tttggttgcg tagcttgcca
     5401 aagtttctgc tagtctgccc cataggacca tattgacaaa atcagcttca cgttcaccgt
     5461 tttggtcttt gtaacgacgg ttcacagcga tagttgctcg cgctaccgac ttgtcattgt
     5521 tggttttgtg caattctggt gtagacgtta aacgcccaat catgataact ttattataca
     5581 tattttcttc ctcctactta tctattcgta ggaaatcaaa aaaagttaca gaaatttgta
     5641 acttttcgag gaaatttctt attttttatg aatcatgaaa cctgtcgcct gttgattggc
     5701 cataatggtc atatctgtaa tctgaacacg gcgaggctga ctggtcacat agactactgt
     5761 gtctgcaata tcctgagctt gcaaggcttc tattccttgg taaacggacg cagctcgctc
     5821 tttatcacca tgaaaacgaa cagttgagaa atctgtttcg acaatcccag gctgaatggt
     5881 tgtcaccttg atatccgttg cgatggtatc aattcgcagt ccatccgaaa aggtcttaac
     5941 cgcagccttg gtagctgagt aaacagcggc accagcatag gcgtagattc ctgcggttga
     6001 ccccatattg ataatatgac cttgattgac ttttatcatt gctggcaaga aacagcgagt
     6061 aaccgccatc agacctttaa cattggtatc caacatggtc aacatatcca actcctcata
     6121 gtcttgatag ggagccaagt caagagccag tccggcgttg ttgaccaaga tatcaatcct
     6181 ccctatcgtt tctagaatat cagagcaaac agtcttcacc atgaccatat ccgtcacatc
     6241 tagtggaaaa gtccagacgg tttgatttgg aaagcttact gcaaactccg acttgaggat
     6301 ttctaatctg tctatccgtc gtcctgttag aacgacatcc tcaccctgct ccagataagc
     6361 acgcgcaatc gcttcaccga ttcctgaggt tgctcccgta atcactacat tttttgccat
     6421 cttatttcct tctatcagat gctgacaagt tcctaggcag tccagtgttt cgctgggtcg
     6481 aacggtgttc cgacaacttg gtcttctgat aattcaagca ccccacgttt ttgcggagca
     6541 tttggcaaat gcaattcacg aggactacac atcatcccaa aactcttttc accacgaagt
     6601 tcgcctggga aaatgaaatt cccttttggc atcatggcac caggaagagc cacaatggtt
     6661 ttcaacccaa cacgcgcatt aggtgctcct gcaacgattt gcactatctt gtcacttgcg
     6721 actgcaactt ggcagatgtt gaggtggtca ctgtctggat gagctaccat ctcgacaatt
     6781 tcaccaacaa caaacttagg ttccttatca ttaacaattt cttctgtaaa accttccgcc
     6841 tgtaattctt ggttcaaacg agcgacttgc tcatctgata aaaagacttg accgcgctct
     6901 gcaatttcaa acaagctcga aacctcaaaa atgttccaag ccactgtttc cccattttct
     6961 ttgaggaaaa cacgggctac cttgcctttg cgttccacat tcagtttggc atctccgcta
     7021 tttttcacga tgaccataag gacatcactg acatgttctt tgttatacgt aaaaatcatt
     7081 gtttcctttt tctcctattt caatcctgct aaaaaatcgt taatttgctc cttactttta
     7141 cggtcgcgat tgacaaaacg accgatttcc ttgtcctttt ctagaacaac aaggctagga
     7201 attccgtaaa catcccagag tttggccaaa tccatatact gatctcggtc cattcgaata
     7261 aaggtgaact ctggattggt ctcttcaatc tctggtaagg caggataaat ataacgacaa
     7321 tcgccacacc agtctgccac aaaaaggaag accttcttgc ccgctttttc cactaaagat
     7381 gctaattctt ctaaacttgc tggctgtatc ataagacttc ctcctcatag actaggtctt
     7441 cattttcata gacaaaggta taatgacggc catcctcaaa aatgacgcca ccaaccaagc
     7501 tctccagact gctttcgtaa acttgaacat aaagggtcgc aatttccccc atgtcggaaa
     7561 aatggtctcg cacaatctct gtcaactctt cctgagtctt catgagctta cggtcatctg
     7621 caactttttt cgtagcaaga gcaaggcttc cgatacctag cagagccaag cctgccatcc
     7681 acattttttt agctttcata ccattcattt taacacaaaa aaggcttcag gacaaatgag
     7741 gaagcagcag aaaagcaagt aaaaagcctc ttcctttaag gaaaaagact tcttatacta
     7801 agagcaaact aggaagctag ccgcaggctg ctcaaaacac ggttttgagg ttgtagataa
     7861 ggcgacgttg acgtggtttg aagagatttt caaagagtat tattcttatt gccaggcacc
     7921 taagttgcca acgtagtaac tatcaggtgt gtaggtattg cgaagcatct tacctgatga
     7981 agccagataa tactacttgc cattgtcttt gacccaatca ttcgcaatca tggaaccaga
     8041 agaacttaca taataccatt ctcccttgtc ataaacccaa gtactgactt tcatggttcc
     8101 tgagcaatta aaggcaaaaa aactgtccaa tatcattcgt tttttaaaag catttgacac
     8161 tacatcatct acgctgtagt acatagatac atcaaacgta aaagccaaaa tatcgaaaaa
     8221 tatacaactt ttaaaggcag catttcattt tttaaagtct aacaaccata gaactgaaat
     8281 cagagcaagg catcaatttc ctactcttag tacgtctatt ttgatggttg aagtgaagat
     8341 ttttttattt aacgtagaca atttccaact tcccaaaagc cacatctcca cggatagtca
     8401 aggttttgct ggttggggct acaggagcgt cagccttagc tgcaccaaag gaggtttcta
     8461 ctttcaaatc tacacgccag tgttgtggaa catagacggt tgcattcccg aaggccactt
     8521 caatatttaa agttgcaaaa tcacctagca tctctgcatt atcatagtag attttagcat
     8581 ccccaaaagc gacttccact tggtcatcta cgagatcttg atcttgctta tagaaggtcc
     8641 cgctaccaaa agcgacttcc ttatccgtga cgactgtttt tttcccattg taccaccatt
     8701 ttttttcatt ccagaactta cttgaatgcg tcagataacc aacaccaagt accaccaaga
     8761 tgctagccca aataagagaa tgattggtaa ctggtaacaa gtcataagcg taatttgcaa
     8821 tgatgagcgc cagtaaacct gtaaaaactg ccgaagtgag atgacgtcta aggatggact
     8881 caatggactt ataagcaaaa aaaacaatac ctagtaaagg ccatatttta ccatccaaag
     8941 aaggaattcc aaaattccct tgcagcaaga tccaagctgc taaaaccaac aaaacaatac
     9001 caaatgcttt ctttttcatg ttttttacct catgtttctt agattttctt ttaggaggga
     9061 ttggtaatgc cgtgagacat gaacctcctt gtgcgtctga taaaaggaaa tggtgcccgt
     9121 tcctgaaaag gacttgtcca ctgagtaaat ctgacggatg tttgcgatcg ttgacttgga
     9181 aactcgatta aaatagcgag gcaagataga ctccaactca tagagcttga ggcgaacctc
     9241 ataagcttcc ttctggttat gagcgtagat cttgctccct tctgtttcaa agaagagaat
     9301 ttctgacagg tctagataat attcacctgt ccccttgtaa aaagtcaacc tcggagcctt
     9361 tgactcttgc aagagccgtt gcaagtctgc aatttcatct gtcaaagtgg gggtcttgat
     9421 gacaatttca gtctcctcta aattgccgtc aatctcaatt cgtaacttca tcacatctcc
     9481 tttctgactc tactatatca aagctaagtt aggaatacaa gggcaaaaaa gcaagtggtc
     9541 aatttgaagc agtaagtggt tgtaggacta gcttaactta catgtaagaa taggaagaaa
     9601 aggaatcccc acaccaaacc acaagcatag ccaaccaaga catccttggg ataatgaaca
     9661 ccccctaaaa ccctcaccaa ccccagcaaa gctgaaaaga tcaagcatac taagccaata
     9721 ggtagactag catgcagaca ggccatggag atgatagttg ccgaaaagac atggcgactg
     9781 ggcatcgacc gtcccgaact atccttatca agaaggggac agatatccca agtttcataa
     9841 ggtctcgggt gattgatttt cttacgaaac agggacaagg ccacaaaacc tgatgcagga
     9901 ataagcacac aaaccccaac ttgctgacca agtcctagtt gcaggtaagt ggtgactagc
     9961 aagatcagat agaccagggg catggcgact gtcatcaaac gattgaaact gcgtaatagg
    10021 aataaaagaa ttggacgact agttagtctg gagctgatat ttcgatacca ttcttgataa
    10081 tttttcattt atgcttctca tacttttcta gaacttgttg agatgttttc cccttggcaa
    10141 gccaatccac tagcttgtcc aagcgacgta tttcctgcat cagaggatca tcaatttcct
    10201 caatacggac actgcaaatc ttccctgtaa ttgcaattcg ttcagggaca taatatggtg
    10261 cttgtctaaa aaaatctccg taagtaacat ccctatccag acaagcaaga acatcactca
    10321 cttcataccc tgtcaaccaa ctggtcactt ggtgaactga ctctgccttt ccaccctttc
    10381 gctcaacttt tgcaatcaag gctaagtaga cagcagcaaa tttcatattg tataatttct
    10441 gactcatact tctctctttc tatgtgtact agtataaata agaaaaagaa ggccgtcaag
    10501 ccttcttttg atttattctt ctgcttcatc ttctgtaaat tgactattgt acaagtcagc
    10561 gtagaagcca ccttgcgcca tcagttcctc atagttgcct tgctcgatga tatttccatc
    10621 tttcatgacc aagatcaagt ctgcatttcg gatggttgac aagcggtggg caatgacaaa
    10681 ggatgtgcgt ccttccatca aacggtccat ggctttttgg atcaattcct ctgtccgtgt
    10741 gtcaacagaa gaagtcgcct catccaaaat caaaagcggt gcatccttaa gaagggcacg
    10801 agcaatagtc aatagttgtt tttgtcctac agacaaggtc acggtgtcat ccaagatggt
    10861 atcatagcca tctggcaagg tcataataaa gtggtgaatt cccacagcct tactagcttc
    10921 catcattcgt tcatcactaa tccctatttg attatagatg agattgtctc gaatagttcc
    10981 ttcaaagagc caggtatcct gcaagaccat tgaaaaggca tcatgcactt ctgaacgcgt
    11041 catagccttg gtatccacac catcaatgtg aatacttccc ttatcaatct catagaattt
    11101 catcaaaaga ttgacaatgg ttgtcttacc agtcccagtc ggcccaacaa tggcaacctt
    11161 ttgaccagca tgaactgtcg cagagaagtc atagtcttga acattgacac cgtccaccag
    11221 aatttctcct gctgacacgt cgtagaaacg tggaatcaga ttgaccagag ttgatttacc
    11281 agaacctgtt gacccaataa aggccactgt ttgaccagtt tctgctttaa agctaacatg
    11341 ttcaataact gcctccgaat ttgccgcata gcggaaggtc acatccttaa actcgacctg
    11401 acctttgaag ttttcatcag tcagctgcac ttgaacaggg ttttggatag aagaatgcaa
    11461 atctaaaact tgattaatcc gcttagcaga gaccatagtt tggggaagaa cgatgaagag
    11521 tgctcccatg agaaggaagt ccatgacaac ctacatggca taagacatga aaacaatcat
    11581 gtcactaaag agaggcagac gcgctatcgg agcagcgtcg ttaatcacat aggccccaat
    11641 ccagtaaatc gccacactca aaccacttga aatccccatc atgataggat tcaaaatagc
    11701 cataagacgg ttgacaaaca aatttaaacg ggtcaattca tcatttactg ctgcaaattt
    11761 ttcattttga taatcctctg cattgtaggc acgaacgaca cgaatacctg ttaaactctc
    11821 acgagtgata ctgttcagtt tatctgtcag cccctgaatc aaggactgtt ttggaaaggc
    11881 tagcgtcatc aaaacggtcg tcatcaggac gttgacaatc actgccacaa gtacggccca
    11941 gagccagtat tctgaatgac ctaaaatctt cccaatagcc cagatagcca taattggacc
    12001 acacgttacc acttgcaagt ccatagtaat caacatttga acttgagtaa tgtcattggt
    12061 agtacgcatc aagaggctag gaattgaaaa tttcttaatc tctgtctgcg agtaatccaa
    12121 aactcggtta aaaatatcac ttctcagcct actagtataa gaagccgcca ctcgggatgc
    12181 aaaaaatcca actgcaacta cggacaagaa ggcaagaaag ggcattccca tcatcatgct
    12241 tgccggctgc cacaactcat ctaaattagt tccttgacta cctagcaaat ccgtaatttt
    12301 cgagatatag gtcggcactt ccaactctag atagaccgaa aagcaagtaa agagaatggc
    12361 tagtaaaatc atcccccatt cttttctact aattcttttg actaatttct ttattctctc
    12421 ctcctattcc cttgatattt gcctgtagtt gaccgagaac tttctcaaaa atcagtaatt
    12481 catcttcatc aatgtcttcc atcaactgct tgtctatgcg ttcaaaaaaa gccttaacct
    12541 gttgcatctg agaacgtgct ttgtccgtca gacgaacaaa cttagcccgc ttatcaacag
    12601 gactcgcctc caattccacc aaaccatttt gcactatacg cttaaccaga ttactagcaa
    12661 cagacttggt aatattgagt tcctgctcga tatctttaat caagaccaag tcttggtttt
    12721 tctcgcgatt atccaaaaaa tgcacaacct gaccttgcgg cccacccata aattcaatgc
    12781 cgcaacgttt ggcttccttt tgcaccatca ggtgaatttg atgaccaaaa cgcttaaaga
    12841 ctaacatcgg tttatccata atctccccct tctaaataaa aatagttctc tggagaataa
    12901 ttaaatttct atgagaacta ttttcttgat taaaaaaatc ccaagtgatt ttctcactta
    12961 ggatcatgtt ctataggtta aattaaaacc catctacgtt cgtataaatc ttttggacgt
    13021 cttcgtcgtc ttcaagaacg ctgtaaagtt tttcaaaggt ttcaaggtct tcgcctgaca
    13081 attccacttc tgactgagga atcatttcca attcagtcac ttggaattct tcaataccag
    13141 actcacggag ggcaacgata gccttgtgaa ggtcagttgg cgctgtgtaa actgtgattg
    13201 taccttcttg tgcttctacg tcatccacat ccacatccgc ttcgagcaat tgctcaaaga
    13261 ctgcgtccgc atcttcacct ccaaatacaa taacaccttt gttgtcaaag aggtaagaaa
    13321 cagaacctga agcgcccatg tttccgccgt ttttaccaaa ggctgcacgg acattggctg
    13381 ctgtacggtt gacgttagaa gtcaaagtat ccacaattag catagagcca tttggcccaa
    13441 aaccttcgta acgtccttct gtaaaggttt cgtctgtgtt tcctttggct ttatcaatcg
    13501 ctttatcgat aatgtgtttt ggcacttggg cttgtttagc acggtcgata acgaatttca
    13561 aagctgagtt tgattctgga tctggatcac cttttttagc tgctacatag atttctacac
    13621 caaattttgc atatacttta gagttggctc catctttagc cgttttcttg gctacgatat
    13681 tggcccattt acgtcccatt aggaatctcc ttttttcaca ttttaatctt tcttattata
    13741 acacaagttt ttttgatttt cactagagga aatggatttt attagcaaat caagctagga
    13801 tagcacttta cctgctaaga tggtcttgcc tttctatctt tatcaacaga cactcatcca
    13861 cattcaaaaa acaaactaga ccattatctg caaatagaaa gtttcagcca agtttgacaa
    13921 agtcagctca aattactgtt tgaagtttgt agatataagc gacaaaaaca atcatactgc
    13981 accttttgtt gacagtctac tccagacata tcatagttca agtaaatact ttgaaatcca
    14041 acagttctta taggcgctat tgtattctaa gaaatcaata gaagagtttc taagcaaacc
    14101 tctaatactc aataaaaatc aaagagcaaa ctagaaagct agccgcaggt tgctcaaaac
    14161 actgttttga ggttgcggat agggctgaca tggtttgaag agattttcga agagtataat
    14221 ttacgtgttc ctaagatgga gaagttagac tagtacactg gcacttctaa accattgcta
    14281 gcaattgatt tgttcatatt taatttcatt ttttccataa atgggtatta gatataaaca
    14341 gcaaaatatt tccgatacgt gtcattcttg aatttccaat catctaaaac aagtaaagga
    14401 taatcaatcc cctgtatatc aaggaattgg ctaccctttt tactttttta cacatcctgt
    14461 ttgatagact cattttaaca tcacgagcat actccaatgg aaatcgctag gcaagagata
    14521 aactttcaga tattcgcaga gagctcatcg cctctttttg tcgcaaacat tcttctctcc
    14581 taatcatttt ctaccttatc ctctacctga ggatagagag ttgttcccca aatagaaatc
    14641 gtccgcttac gcactagtgg caaatcggtt ttttcataaa ccgtacgcca ccattcccag
    14701 gcaagcccgg tacactctct aattttgaca gagagattgc gaacattccc ttttaaagga
    14761 atactagtgg taaagtgagc cgtcaaatcc tgcccatttc tgtcccaagc cttaggagtc
    14821 aagacttcct tgccttgatg atcataggat aattcatccc aagtaatata atattgggca
    14881 acataggcac cactatgatc cagcagtaaa tctccgtttc tgtaagctgt aaccttagtc
    14941 tcaacatagt ctgtactgtt ttgaaaggtc gcaactacat tgtcacgtaa aaaagaagtt
    15001 gtataggaaa tcggcaagcc tggatgatcg gctgtaaagc gactgccttc ttgaatcaag
    15061 tcctctacca tatccacctt gcctgttaca actcgggcac ccgaacttgg gtcgccccct
    15121 aaaataaccg ccttcacttc tgtattgtcc aaaatctgct tccactctgt ctgaggagct
    15181 actcctttta tcaaagattc aaaagcagcc tctacttcat cactcttact cgtggtttcc
    15241 aacttgagat agacttggcg cccataagca acactcgaaa tatagaccaa aggacgctct
    15301 gcagaaattc ctctctgcct taaatcctct accgttacag tatcttgaaa cacatctcct
    15361 ggatttttaa cagcatctac gctgactgta taataaatct gcttaaaatt aacaatctga
    15421 atctgctttt cgcctgaatg gacagagtta aaatcaatat caagagaatt ccctatcttt
    15481 tcaaagtcag aaccaaactt gaccttgagt tgttccatgc tgtgagccgt gattttttca
    15541 tgctgcattc tagctgggac attattgacc tgaccataat cttgatgcca cttagccaac
    15601 aaatcgttta ccgctccgcg aacacttgaa ttgctggggt cttccacttg gagaaagcta
    15661 tcgctacttg ccaaaccagg caaatcaata ctataagtca tcggagcacg atcgaccgca
    15721 agaagagtgg gattattctc taacaaggtc tcatccacta cgagaagtgc tccaggatag
    15781 aggcgactgt cgttggtagc tgttacagaa atatcacttg tatttgtcga caagctccgc
    15841 ttctttcttt cgataacaac aaactcatcg ggtagctgat taccctcttt gatgaaacga
    15901 ttttcaatac tttctccctg atgggtcaag agtttctttt tatcgtaatt catagctagt
    15961 ataaagtcat ttactgcttt atttgccatc ttctacctcc taataagttc ctggattgag
    16021 ttgcataaac tcagacttgt tcagcgaaat cagccgtggt tggactaagt aatccaaaat
    16081 ttcctcgtac aattcttctg agacattgcg tcgccgtctg gctaaataag aagtcggaat
    16141 gaccgtatta tccaacataa ataccttatc taagtcaatc aaggttggtc ttgtaaaagg
    16201 attacgagct agatccggct cttctatcat aaagttcttg accaaacgtc tggtcaagag
    16261 agctggtttg aaggtctgat ttttaaccaa ctctttgttt ttagtcatgc tgttgtcaat
    16321 acagatatac atatgattct tcacagccaa atcgctacta atagtcggaa aaggcaaata
    16381 aagagctaca acatctcctc tcttaatcaa gcaagagcac ccccttttct cctaatgtaa
    16441 catagacagg attgaccaag tcttctgatt gactcagaat ttccaaagtt tgagtttggc
    16501 gcgctgtcaa tttagtagca tcttgtctct tcaatacaaa atgcttgtcg ccaataacct
    16561 tgacaatata atccttctcc aaagctgact ggtaaatcca catcagatgt tgtctgtcct
    16621 gagaactcaa gagagaagga ttttcaagcc tcccgatagt ctgataaaaa tcaaaaacag
    16681 gagctaactc ctgccaatct gattggctag ttgtcaaggc tagaaaaagg gctttgcgag
    16741 ctgatacttc ttggttagcc ttgagagtta ctttcccctc caagtttttt agaaatcggg
    16801 aaactccaga aagcaaattt ttctctaact gcgagaaata aaaacctttc gttcccagac
    16861 ataagtcttt catgtcgctt tctctagcaa ataagagctc aaacatttga tagtaaaaga
    16921 aaaatatctg gcactgggtc gcgctcatct tttccttatc ggcttctttt tttaaccaga
    16981 gcaagggcga caggtagctg gattgagaca tttcctctac ctcctactct tttttaactg
    17041 gagcatctgc actagctgcc acttcttttg actggatact ttcccactgg ttaatctcct
    17101 ctgagataag accttcgcat gtcttgacaa atagggcaaa agccttggtc tttcctgcat
    17161 atttctccgt ttggcattga tagaggaatt tttctttctc caggagttgc gcagtttttt
    17221 ggtaagaaat ccaattttcc tttgcattat acaaattgat aatcccctca cacagcaagc
    17281 cgagactgga taaggcaacc gaaatcaaac ggtagcgatc acctggcata ggaatagcac
    17341 aaaagacagc tatgaggaaa cctgccacga tttctgttat ttttaatacc ttatagcgcc
    17401 tacgatgttg aacgcttttc tttaaaaaat gagctatctg tacgtctaat cgctctgtca
    17461 ggtacatttc ttctggcgtc atattcgtaa ctcctttcat ttactttgat aaccatagta
    17521 taacatattt gaatatcaaa gtatagtaaa aactggacta taacttatta gactccctgc
    17581 gaaacaaaat atggtatagt agttctatga attatgaagc aagtaaacaa ttaactgat
//
LOCUS       DAVBCB010000047        17572 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVBCB010000047 DAVBCB010000000
VERSION     DAVBCB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17572
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.047"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(179..1114)
                     /locus_tag="VQU28_001579"
     CDS             complement(179..1114)
                     /locus_tag="VQU28_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HET2476910.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1111..1971)
                     /locus_tag="VQU28_001580"
     CDS             complement(1111..1971)
                     /locus_tag="VQU28_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HET2476911.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFYYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2079..4241
                     /locus_tag="VQU28_001581"
     CDS             2079..4241
                     /locus_tag="VQU28_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HET2476912.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIILSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYFYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4333..5592
                     /locus_tag="VQU28_001582"
     CDS             4333..5592
                     /locus_tag="VQU28_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2476913.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADDTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRSE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5606..6472
                     /locus_tag="VQU28_001583"
     CDS             5606..6472
                     /locus_tag="VQU28_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2476914.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAVFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            6486..7319
                     /locus_tag="VQU28_001584"
     CDS             6486..7319
                     /locus_tag="VQU28_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2476915.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKSTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            7320..7400
                     /locus_tag="VQU28_001585"
                     /pseudo
     CDS             7320..7400
                     /locus_tag="VQU28_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            7538..8980
                     /gene="gtfA"
                     /locus_tag="VQU28_001586"
     CDS             7538..8980
                     /gene="gtfA"
                     /locus_tag="VQU28_001586"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HET2476916.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGEKYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTEILPEIHEHYSIQFKIADHDYYVYDFTLPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAREVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVSF"
     gene            9118..10311
                     /locus_tag="VQU28_001587"
     CDS             9118..10311
                     /locus_tag="VQU28_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HET2476917.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            10998..12977
                     /locus_tag="VQU28_001588"
     CDS             10998..12977
                     /locus_tag="VQU28_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2476918.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13044..14540
                     /locus_tag="VQU28_001589"
     CDS             13044..14540
                     /locus_tag="VQU28_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476919.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14540..15466
                     /locus_tag="VQU28_001590"
     CDS             14540..15466
                     /locus_tag="VQU28_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476920.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15475..16542
                     /locus_tag="VQU28_001591"
     CDS             15475..16542
                     /locus_tag="VQU28_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476921.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16553..17479
                     /locus_tag="VQU28_001592"
     CDS             16553..17479
                     /locus_tag="VQU28_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476922.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
BASE COUNT         5175 a         3141 c         3674 g         5582 t
ORIGIN      
        1 aggctccata atatccatag ggaatttacc cactacaaat attatagagc ccgttttaat
       61 gagagtttgt aactctcttt tttgttttta gagacaataa aacttcagat ccgtaaaact
      121 taattgaatt actaatctga agttattttt atatcgttta tattataatt gtgttatttt
      181 acttccaact attgagagaa atttcgccac tatttagcca gatttctttt ccgttatcac
      241 attgaactaa aagttttcca ttttctgaga tgtctttagc aagtcccttg tagtcttttt
      301 gctctagtgt gaaagtgact tcttttccta gaatgaatga ctgttttttg tataggtata
      361 atagctcttc tgctggtgtt tcgaagaaag cacgccagat ttctatgatc aattcattcc
      421 ttgttatagg agctgtagct ttaaataagc tggcagcttt ttcttttaat tcctgaggga
      481 agtctttaat agtgaaattg atacctactc caataatgat atctgtgact aagccagttt
      541 ctacagaggt cattgcttca gtaaggattc ctccaatttt atgattgttt agatagatat
      601 cattgaccca ttttatgtcg acatctatta aagttaggtt cttaatggct ttgtagacag
      661 ctccagctac aagtagtgtg taggatggta atttgtcata ggggagattt ggtttaagat
      721 ggagtgtcat ataaatacca ccttgtggtg agtagaagga acgttgaaaa cggcctcggc
      781 ctgctgtttg ataggaagct agatagaggg tatttgcttc atggcctaaa tcaattgctt
      841 cttttgcatc tagttgtgtt gattttgttt cgggtttaaa gctgacttta attggaagat
      901 tttcttctag aatctctgga agaataaggt caccattcat cagtttatat cctctatttt
      961 tgatactatc aatttcaatg ccttcttgtt ctagtcgctt gatggctttc caaattgctg
     1021 ttcggcttag ggatagtttt tctgcgattt tttctccgct gatatagtcg gtttctttag
     1081 ataggatttg gtagacagct tggtaggatt tcataatgtt gcctttctca ctagttggta
     1141 ttgagagtat tcttttcttg tatgacttgg agtctgatta aagtattgtt tataagcttt
     1201 cgaaaaatgg agtggatctg aaaaacctac cgagtatgca attaccttga tggactcttg
     1261 ggtattttcg agaagttgtc tagctcggtg cattcgaacg tagagtaggt attctttggg
     1321 tgataaggta ttaaattctt tgaatacgct tgataagtag cttctgtgaa cggatagttc
     1381 ttttgctaaa tcttgaattg taagtgattg aggatagtgg ctatcaatta atcgtttgca
     1441 ttcaagatag agttggtggg ttgatgaaat attctttttt ttctgattgg gagcaatagt
     1501 tcccagatga aacatcagtt catgaagttg tcccatgata tggagttgag ctaattcact
     1561 tgattttgta atctgagcga agcggacaat gtctgagatg agttttgcag tagtctgggt
     1621 atgacaagtt tcagattgga tgagatagga ttgatcagaa atttgagaaa gagcaaaata
     1681 atcaggggat ttccctccag tgattcctaa ccagtagtag gcccaaggtt ctttactatc
     1741 tgcttgataa aaggttagtt cctctggttt taatagaaag aaatctcctt cttttaaatc
     1801 aacaatttta cccttgtaat aaaattttcc ttgtccttta gtaatgtaat gtagaacgta
     1861 tgtatcacga atggctggac caaaagagta attaggtgtg cattcctcat atccataaaa
     1921 gcttagggca aggtcgattg ttccagtctg gtattctgaa aaaactaaca tgtgctacct
     1981 cctacctaac attttaccat attcttgaga catttttcta ttttggaaag cgatttcagg
     2041 tgataaaata taatcaataa agtgatgagg tgaagtaaat gggagttagg atagagaata
     2101 atctatttta tgttgagagt aaaaatctaa gtttgattat tgaaaatcga aatggctact
     2161 tacttttgaa acatttagga aagactatta agaactataa aggttccaat agtgtttatg
     2221 aacgagacca tgccttttca ggaaatccaa cggctactaa tcgaaccttt agtttagata
     2281 ctcagcgaca gatttttgga caacatggct taggagattt taggaaacca actatacagg
     2341 ttcagcatag tgtaactgaa gtaacagact ttcgatttgt agaagcaaag attttaaaag
     2401 gtcagaatgg tccacagggc ttaccttctc cacatagcat ggacgataca gagactcttg
     2461 tcttaatgtt agaagattct aaggctcaac ttagtctgac tttgtattat actactttta
     2521 ataatgatgc gactattact agctacagta aattagataa taatagtaat caggaagttg
     2581 tcatccataa agatttttct tttatggctg attttccagc tgcagattac gaaatagcaa
     2641 ctctgcaggg tgcttatgct cgtgaaaaga ctgttagacg tcaacaggta gaacaaggaa
     2701 tcttttcgat tagttcaaac cgaggtgctt ctggtcatgc tcaaacacca gctcttctac
     2761 tatgcgaaca aggagtcaca gaggatgctg ggaatgtgtt tgctattcaa ctaatgtata
     2821 gtggcaactt tgaagctttt gttcaaaaaa atcaattgaa tgaagttcgg gtggctattg
     2881 gcattaatcc agaaaacttt tcttggaagt tagcccctga ggaatacttt gaaacaccgg
     2941 tagctttagt gactcattca gatcagggat taactggtat tagtcatgaa agtcagaatt
     3001 ttgtactgaa gcacattatt ctaagtgaat tttctaaaaa agaacgtcca attctaatca
     3061 ataactggga agctacttac tttgactttc agagagaaaa actgttagag ttagcagatg
     3121 aagctaagaa agttggcatt gaactttttg tattagatga tggttggttt ggcaatcgtt
     3181 ttgatgataa tcgtgcttta ggtgattggg ttgttaatga ggaaaaactg ggtggaagtc
     3241 tagaaagtct gatttcagct atccatgaaa gaggtttgca gtttggactt tggttagaac
     3301 ccgaaatgat ttctgtagat agtgatttgt atcgtcaaca ccctgactgg gctattcagg
     3361 ttcctgacta tgagcatact tattctcgga atcaattagt acttaatctt gccaatcctc
     3421 aggtagtaga atacttgaaa agtgtcttag atcaactcct atcttatcat gagattgatt
     3481 acattaaatg ggatatgaac cgcaatatca ctaagctagg gaatggatta acttatctag
     3541 agacacagat gcaatctcat cagtacatgc tggggcttta tgaactcgtt tcttatctga
     3601 cagagaagca cagccatatt ctctttgagt cctgctctgg tggtggtgga cgaaatgatc
     3661 ttggtatgat acgctatttc ccacaagtct gggctagtga taatactgat gccattgcac
     3721 gtttaccaat tcaatacggt tcatcctatt tctatccaac catttctatg ggggctcatg
     3781 tgtcagcagt accgaatcat cagatgggac gaatgacacc attagaaaca cgtggccatg
     3841 tagcaatgat gggaaatttg ggctatgagc ttgatttgac aaatttatca gatgaagaga
     3901 aagctacgat tgctaatcag gtgaacttgt ataaagaatt acgaccagta gttcagttag
     3961 gacagcagta tagactaatt aatcctgata ctgcatccaa tgaagcagct gtacaattta
     4021 attacggaaa tcaaacgatt gtaacctacg ttcgcgtttt atctgttgta gagaccatgg
     4081 aaacaacttt aaaattaaaa gatttggatg aagagggact atataaatta caggaaaatg
     4141 gcgaagttta ctcaggtgca gaactaatgt atgcgggctt aactgttatc ttatcccaag
     4201 gagatttttt gagtagacag tatattttta gaaaactatg atatgtactg accccaaaag
     4261 ttagatttat tctatctaac ttttgaagtg cagtttgata agggtgtata aatttataaa
     4321 ggaggctttc taatggaatg gtataaaaaa atcggacttc ttgcaactac aggtttagct
     4381 ttgtttgggc tcggtgcttg ctccaactat ggtaaatctg cggatgacac agtgaccatc
     4441 gagtatttca accagaaaaa agaaatgacc aaaaccttgg aagaaatcac tcgtgatttt
     4501 gagaaggaaa accctaagat aaaggtcaaa gtcgtcaatg taccaaatgc tggtgaagta
     4561 ttgaagacac gcgttctcgc aggagatgtg cctgatgtgg tcaatattta cccacagtcc
     4621 atcgaactgc aagaatgggc aaaagcaggt gtctttgaag atttgagtaa caaagactac
     4681 ctgaaacgcg tgaaaaatgg ctacgctgaa aaatatgctg taaacgaaaa agtttacaac
     4741 gttcctttta cagctaatgc ttatggaatt tactacaaca aagataaatt cgaagaactg
     4801 ggcttgaagg ttcctgaaac ctgggatgaa tttgaacagt tagtcaaaga tatcgttgct
     4861 aaaggacaaa caccatttgg aattgcaggt gcagatgctt ggacactcaa tggttacaat
     4921 caattagcct ttgcgacagc aacaggtgga ggaaaagaag caaatcaata ccttcgttat
     4981 tctcaaccaa atgctattaa attgtcggat ccgattatga aagatgatat caaggtcatg
     5041 gacatccttc gcattaatgg ctctaagcaa aagaactggg aaggtgctgg ctataccgat
     5101 gttatcggag ccttcgcacg tggggatgtc ctcatgacac caaatgggtc ttgggcgatc
     5161 acagcgatta atgaacaaaa accgaacttt aagattggaa ccttcatgat tccaggaaaa
     5221 gaaaaaggac aaagcttaac cgttggtgcg ggagacttgg catggtctat ctcagccacc
     5281 accaaacatc caaaagaagc caatgccttt gtggaatata tgacccgttc agaagtcatg
     5341 caaaaatact acgatgtgga cggatctcca acagcgatcg aaggggtcaa acaagcagga
     5401 gaagattcac cgcttgctgg tatgaccgaa tatgccttta cggatcgtca cttggtctgg
     5461 ttgcaacaat actggaccag tgaagcagac ttccatacct tgaccatgaa ctatgtcttg
     5521 accggtgata aacaaggcat ggtcaatgat ttgaatgcct tctttaaccc gatgaaagcg
     5581 gatgtggatt aggagtagag agactatgaa aaaagtatta caaaaatatt gggcatgggc
     5641 ttttgtggtc atccccctct tgttacaagc aattttcttc tatgtgccga tgtttcaagg
     5701 agccttttac agttttacca actggacagg attgacttac aactacaaat ttgtcggctt
     5761 aaacaacttt aagctcctct tcatggatcc aaaattcatg aatgcgattg gctttaccgc
     5821 aatcattacg attgccatgg tggttggtga gattgcactc gggatcttca ttgcgcgtgt
     5881 cttgaactct aaaatcaaag gccaaacctt cttccgtgct tggttcttct tcccagctgt
     5941 tttatctggt ttgacagtgg ctttgatctt caagcaagtc ttcaactacg gtcttccagc
     6001 gattgggaat gcccttcata ttgaatttct ccaaaccagt cttttaggga ctaagtgggg
     6061 agcaatcttt gcggctgtct ttgtccttct ttggcaaggg gtggctatgc ccatcatcat
     6121 cttcctagct ggtttgcaat ctattccaac tgagattaca gaggcagcaa ggattgatgg
     6181 tgcgactagc aagcaagttt tctggaacat tgaattgcct tacttgctac caagtgtctc
     6241 tatagtcttt atcctagccc taaaaggtgg gctgactgcc tttgaccaag tctttgccat
     6301 gaccggtggt ggtccaaaca atgccacaac ctcacttggg ctcttggttt ataactatgc
     6361 ctttaaaaac aaccaattcg gttatgccaa tgccattgcc gtaatcttgt tcttcttaat
     6421 tgtagtgatt tcgatcatcc aattgagagt atctaagaaa tttgaaattt aagaggagaa
     6481 gcatgatgaa acaagatgaa agaaaagccc tgattggcaa atacattcta ttgattctag
     6541 gatcggttct gattttagtg ccgctccttg ctaccctctt tagttccttc aaatccacta
     6601 aggatattgt agataatttc tttggctttc caaccaactt cacatgggac aactttagcc
     6661 gtctcttagc tgatgggatt ggaggctatt attggaactc tgtcgtcatc actgtcttgt
     6721 ctttacttgc agtaatgatc tttatcccta tggcagccta ctccatcgct cgcaatatga
     6781 gtaaaagaaa agcctttacc atcatgtata ccctcttaat cctcggaatc ttcgtacctt
     6841 tccaagtcat catgattccg attacggtta tgatgagtaa actcggtttg gctaatacct
     6901 ttggtttgat cttgctctac ttgacctatg cgattccaca gaccctcttt ctctatgttg
     6961 gctatatcaa aatctcgatt ccagaaagtc tggatgaagc agcagagatc gatggggcta
     7021 atcaatttac aacctatttc cgcatcatct tcccaatgat gaaaccgatg catgcgacaa
     7081 ccatgatcat caatgccctt tggttctgga atgacttcat gttgccactc cttgtcttga
     7141 accgggattc caaaatgtgg actctgcctt tgttccaata caactacgca ggccaatatt
     7201 tcaacgacta cggaccaagc tttgcctctt acgtggtcgg cattatcagt atcaccattg
     7261 tctatctctt cttccaacgc catatcatcg caggaataag caacggggca gtgaagtaat
     7321 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt
     7381 ttgaggttgt ggatgaaact gacgaagtca gtaaccatat ctacggtaag gcgacactga
     7441 cgtggtttga agagattttc gaagagtata acatgaattt gatttacgaa gccagatcat
     7501 atgagacttg gcttcaatta gaaaaaggag agtaatgatg ccaattcaaa ataaaaccat
     7561 gttgattacc tattctgata gccttggaaa taatcttaaa gacttatatg ataatttgga
     7621 agagcatttt ggagatgcta ttggaggagt tcacctttta ccatttttcc catcaacagg
     7681 tgatcgtgga tttgcgccag ttgactacga cgaagtggac tcagcttttg gtgattggga
     7741 ggatgttaag cgtttaggtg agaaatatta tcttatgttt gattttatga ttaatcatat
     7801 ttctcgtcaa tccaagtatt ataaggacta tcaagaaaaa catgaagcca gtgaatttaa
     7861 agatctcttt ttaaactggg ataagttttg gccagaaaac cgtccgacac agtctgatgt
     7921 agatttaatt tacaagcgta aggatcgtgc accaaagcaa gagattgtgt ttgaagatgg
     7981 ttcagtggaa catttgtgga atacctttgg tgaggagcag attgatcttg atgtgaccaa
     8041 agaagtaact atggaattta tccgtaagac cattcagcac ttggcaagta atgggtgtga
     8101 tttgattcgt ctagatgcct ttgcttatgc agtgaagaaa ttggatacta atgatttctt
     8161 tgtggaacca gatatttggg atttattgga caaagttcga gatatcgctg ctgagtatgg
     8221 gacagagatt ttacctgaga ttcatgaaca ctattcgatt cagtttaaaa tagcagacca
     8281 tgattactat gtttatgatt ttactcttcc aatggtgaca ctttatactc tttacagttc
     8341 cagaacagag cgtttggcta agtggttgaa gatgagcccg atgaagcaat ttacgacgct
     8401 agatacccat gatgggattg gagtagtaga tgtcaaggat atcctgaccg atgaggagat
     8461 tgactatgct tcaaatgaac tctataaggt tggagccaat gtcaaacgta agtactctag
     8521 tgccgagtat aacaacttag atatctacca aatcaattca acctactatt cagcgcttgg
     8581 agatgatgat gtcaagtatt ttctcgctcg tctaattcaa gcttttgccc caggtattcc
     8641 tcaggtttac tatgtgggtc tattagcagg caagaatgac ttgaaattat tagaagaaac
     8701 taaagaaggt cgaaatatta atcgtcatta ctatagcaac gaggaaatag caagagaagt
     8761 gcaacgacct gttgtgaagg cccttctcaa tctattttct ttccgtaacc gttcagaagc
     8821 ctttgatcta gaagggacta ctgagataga gacaccaaca gctcacagca ttgtaatcaa
     8881 acgtcaaaat aaagataagt ccgtaacagc agtagcagaa attgatttgc aaaatcagac
     8941 ttatcgggta attgagaatg gagttgaagt atcattttga agccttgata tggtggataa
     9001 aatagggttt ttattttgga aaacgtttcc tttgttttca aattgctaaa aaagtggtac
     9061 aatataggat agcttactat tatctgaatc agctgatttg gagagaaagg attcattttg
     9121 aaatcaatag gctttattga aaagctgaag gggttgtcta gtaaagagct gattttattg
     9181 ggaattatcc taagtatctt tttacccttt tatctttttg tagttgtact ctgtttatat
     9241 attatcagtt tgatttttac aggagacatg aaaagtattc ttcagaaaat gggggagcat
     9301 ccgatgctgc ttctttttct tagctatagt actgttatat ccattcttgc acaaaattgg
     9361 atgggtcttg tggcttcagt aggaatgttt ctatttacta ttttcttttt gcactatcag
     9421 tcgattttat cccataaatt ctttcgattg attttgcagt tcgtcttgtt tggtagtgtc
     9481 ttgtcagctg cttttgccag tttagaacat ttccaaattg tgaagaaatt taactatgct
     9541 tttctttcac ccaatatgca ggtgtggcat cagaaccggg cagaagtgac cttctttaat
     9601 cctaattatt atggaattat ttgttgtttc tgtattatga ttgctttcta tctgtttaca
     9661 acgaccaagt tgaattggtt gaaagtattc tgtgtgattg caggctttgt taatctcttt
     9721 ggtttgaact ttactcaaaa tcgaactgcc tttcctgcta ttatcgctgg agcaattatc
     9781 tatctcttta cgactattaa aaactggaag gccttttggc ttagtattgg ggtcttcgcg
     9841 attggtttga gtttcctctt ttctagtgat ttgggagttc gaatgggtac tttagactct
     9901 tctatggaag aacgcatttc tatctgggat gctgggatgg ccttgtttaa gcaaaatcct
     9961 ttttggggtg aagggccatt gacctatatg cactcttatc ctcggataca tgctccttat
    10021 catgaacatg cccacagtct ttatattgat acgattctga gttacggaat tgtgggtacc
    10081 attttattag ttttgtcttc tgttgctcct gttcgcttga tgatggatat gagtcaggag
    10141 tcggggaaac gtccgattat cggcctttat ctatctttcc ttacagtggt tgctgtgcac
    10201 ggaatttttg acttggctct cttctggatt cagtcaggct ttattttctt gctagttatg
    10261 tgcagtattc cattggagca tcgaatgttg gtatcggaca tgacggatta agttttataa
    10321 gtttgaaatc ttctaccttg ggtaggagat ttttttatac gatgggcatg tcgtgcatct
    10381 gaggtgtaag tcctctgtag ggatccacta ccggtgaata caatagcgct ccccaagcct
    10441 gactatcgtg aggtagcgga ttaaaatggt ctggggatag atcgttttaa gtctgacgca
    10501 ggaaataaga attgtcagag aaagcaatag tgaaatcgta ggctatgaat agaaacgtga
    10561 tagcgaggag catatagtgg ataaggtagc gagaaatttt aaagtataaa agatagtctt
    10621 aatctatata tgtaaatcac aaaagtaatt gaattcggaa taaggttggt gtgaaaagac
    10681 atagtaaact gaatcttgaa tactagaatg atacttctac aatattgtta taatttaatt
    10741 tgaattccaa aatcacattg ttccgtccgt atttcatttt actgtatgtc ttcctaaact
    10801 ccaaagtttc tgcgtcaaat ttcctatttt cactgtagtc tttgacactt cgaacgacaa
    10861 tttgaatcat atattcaaaa aaaaatgaaa aatttgtgcc aacaagccta aatattctga
    10921 aaatttgttt gatttttaat ggataatgtg atataatggt tgtcaatcta taattcacat
    10981 aaacagagga gtagaaaatg aaaaaaaatc gtgtatttgc tacagcaggt cttgttttat
    11041 tagcagcagg tgtacttgca gcatgcagtt cttcaaaatc atctgattca tcagccccta
    11101 aagcttatgg ctatgtttat acagcagacc cagaaacctt ggactacctg atttcaagta
    11161 aaaatagtac aacagtagtg acttcaaatg ggattgatgg tttattcact aacgataatt
    11221 acggtaatct tgctcctgca gttgcagagg attgggaagt ctctaaggat ggtttgacct
    11281 acacttataa gattcgtaaa ggggttaaat ggtttacctc tgatggagaa gaatatgcag
    11341 aggtgacggc taaagatttc gtgaacggtt taaaacacgc agcagataaa aaatcagaag
    11401 ctatgtattt agctgaaaat tcggttaaag gcttggcaga ttatctatca ggaacttcaa
    11461 cagatttttc aacagttggt gtcaaggcgg ttgatgatta tacgttacaa tacactttga
    11521 accagcctga accgttctgg aactctaagt tgacctattc tattttctgg cctctgaatg
    11581 aagaattcga aacatcaaaa ggaagcgatt ttgctaaacc aacagatccg acatccttgc
    11641 tttataatgg tccattcttg ttgaaagggt tgactgcaaa atcttctgta gagtttgtaa
    11701 aaaatgagca atattgggat aaagaaaatg tccacctaga tactatcaat ctagcttact
    11761 atgatggatc agatcaggag tcgctagagc gtaacttcac tagtggagct tatagttatg
    11821 cccgtcttta ccctaccagc tccaactatt ctaaggttgc agaagaatac aaggacaata
    11881 tctattacac acaatcaggc tctgggattg ctggtctggg tgtgaatatt gatcgccaaa
    11941 gttacaacta tacttctaaa actacagatt cagagaaagt agctactaag aaggcattgc
    12001 ttaacaaaga tttccgtcaa gccttgaatt ttgctcttga tcgctcagct tactcagctc
    12061 aaatcaatgg taaagatgga gcagctttag cagttcgtaa tttatttgta aaaccagact
    12121 ttgtttcagc tggtgagaag acctttggtg atttagtcgc tgctcaactt cctgcttatg
    12181 gtgatgagtg gaaaggtgtg aatttagctg atgggcagga tggtttattc aatgctgaca
    12241 aggccaaggc agagtttgcg aaagctaaga aagctttaga agcagacggc gttcagtttc
    12301 ctattcatct ggacgttcca gtagaccaag catcaaaaaa ctacatatct cgtattcagt
    12361 cctttaaaca atctgtagaa acagttcttg gtgttgaaaa tgtcgttgtt gatattcaac
    12421 aaatgacaag tgatgaattc cttaatatta cttactatgc tgccaatgct tcatctgagg
    12481 attgggatgt atcgggagga gtttcatggg ggccagacta tcaagaccca tctacttacc
    12541 tggatatttt aaaaacaact agcagtgaaa ctacaaaaac atatttagga tttgataatc
    12601 caaatagccc ttcagtagtt caagttggtt tgaaagaata cgataaatta gttgatgaag
    12661 ctgccaaaga gacaagcgac ttgaatgtcc gttatgaaaa atatgcagcg gctcaagcat
    12721 ggttgacaga tagttcactc tttattcctg ctatggcttc ttctggtgca gcaccagtgc
    12781 tttcacgaat tgttccattt actggagctt ctgcgcaaac aggctctaag gggtcagatg
    12841 tttacttcaa atatttgaaa ttacaagata aagtggtgac taaggaagag tatgaaaaag
    12901 ctcgtgaaaa atggttgaaa gaaaaagctg aatcaaatga gaaagctcaa aaagaattgg
    12961 caagtcatgt gaagtaaata atttctaata gaactttctc tccatgagga gaaggttctt
    13021 ttgggatttt taaaggaaat aatatgaaaa aatatatttt tatgcgtgtt ttgcggtcat
    13081 tggtttcgat tttcttagta acgactttga cctacacgat tatctatacc ttggttcctc
    13141 gaaaattgat tttcaagcag gatcctaact ataataaaat tgcaacaacg gctgataaac
    13201 gtgataacta tgaaaatact gtgtttgagc gtatgggcta cattgagtat tacgatacta
    13261 aagagttgca agaaaaggca agtagcatgg attcttctgt aacagtagaa gcaaatgcga
    13321 ccaataaagc tatttatgaa aagtacatca atcaattagg tcatggttgg actttgggag
    13381 aatttactga aagtggtcaa ttctatgcta ctcgtgaaat tccaattttt gaacgtgttt
    13441 ttcacttcta tgctaacttg attgacattg accatacaaa taaaatccaa gaccctgaaa
    13501 atccagactt gaaacgctac cttcgttttg aaaatgatcc agctatcgga tggtcattgg
    13561 tcggttcagg aactaaacat aaatatctct tgtactttaa cagtcagttc ccatttgttc
    13621 atcaaaactt tgtgaacttg aatttaggtg actcttaccc aacctatgct aatacaccag
    13681 ttcttcaggt tattactcaa ggtcaaggac aaaccaaaac tgcccaagtt cagttcccaa
    13741 caggtaagaa aacgtcttct gtaaatattt actcaagaac ctacaagtca cctagtcagg
    13801 ctgactctcg tgaagtagct agctatggga aagatgatcc ttatacagcg actgaaagta
    13861 attaccaata tccatctatg attgtcagct ctgctattac tggtttgatt ggtttggttc
    13921 ttgcctatgc tcttgccgtg ccacttggtt cagccatggc tcgtttcaag aacacttgga
    13981 ttgatagcct ctcaacaggg gctttgacct tcttgcttgc tcttccaacg attgccttgg
    14041 tttacatcgt tcgattgatt ggatcatcta ttgcccttcc agattcattc cctatcttgg
    14101 gagctggaga ttggcgttct tacgttttac cagcagtcat ccttggtttg ttgggtgctc
    14161 ctggtacagc catttggatt cgtcgttaca tgattgactt gcaatctcaa gactttgttc
    14221 gtttcgctcg tgcaaaaggt ttgtctgaaa aagaaatttc aaacaaacac atctttaaaa
    14281 atgccatggt tccgctggtt tcaggaattc ctggtgccat cattggggtt atcggtggtg
    14341 caacccttac tgaaacagtc ttcgccttcc caggtatggg taaaatgttg attgactctg
    14401 taaaagcatc taataactct atggtcgttg gtcttgtctt catctttaca tgtatttcta
    14461 tcttctcacg tcttttggga gatatttgga tgactattat tgacccacgt attaaattga
    14521 ctgagaaagg aggcaaataa tgtctacaat cgataaagaa aaatttcagt ttgtaaaacg
    14581 tgacgatttt gcctctgaaa ctattgatgc gccagcatat tcttactgga aatcagtgtt
    14641 taaacaattt atgaagaaaa aatcaactgt agtcatgttg ggaatcttgg tagccatcat
    14701 tttgataagt ttcatctacc caatgttttc taagtttgat ttcaatgatg tcagcaaggt
    14761 aaacgacttt agtattcgtt atatcaagcc aaatgcggag cattggttcg gtactgacag
    14821 taacggtaaa tcgctctttg acggtgtctg gttcggagct cgtaactcca tcctcatttc
    14881 tgtgattgcg acagtgatta acttggttat cggtgttttt gtcggtggta tttggggtat
    14941 ttcaaaatca gttgaccgtg tcatgatgga agtttacaac gtcatctcaa acatcccacc
    15001 tcttttgatt gttattgtct tgacttactc aatcggagct ggattctgga atctgatttt
    15061 tgccatgagc gtaacaacat ggattggtat cgccttcatg atccgtgtgc aaatcttgcg
    15121 ctatcgtgac ttggaataca acttggcatc acgtactttg ggaacaccaa ccttgaagat
    15181 tgttgccaaa aatatcatgc ctcaattggt atctgttatt gtgacaacca tgactcaaat
    15241 gcttccaagc tttatctcat acgaagcctt cttgtctttc ttcggtcttg gattaccgat
    15301 tacagtgcca agtttgggtc gtttgatttc ggattattca caaaacgtaa caaccaatgc
    15361 ttacttgttc tggattccat tgacaaccct tgtcttggta tccttgtccc ttttcgtagt
    15421 tggtcaaaac ttagcggatg ctagtgatcc acgtacacat agataggagt agaaatgaca
    15481 aaagaaaaaa atgtaatttt gactgctcgc gatattgtcg tggaatttga cgttcgtgac
    15541 aaagtattga cagccattcg cggcgtttcc cttgaactcg ttgaaggaga agtattagcc
    15601 ttggtaggtg agtcaggatc aggtaaatct gttttgacaa agaccttcac aggtatgctc
    15661 gaagaaaatg gtcgcattgc ccaaggtagt attgactacc gtggtcagga tttgacagct
    15721 ttatcttctc acaaggattg ggaacaaatt cgtggtgcta agattgcgac tatcttccag
    15781 gacccaatga ctagtttgga ccccattaaa acaattggta gtcagattac agaagttatt
    15841 gtaaaacacc aaggaaaaac agctaaagaa gcgaaagaat tggccattga ctacatgaat
    15901 aaggttggca ttccagacgc agatagacgt tttaatgaat acccattcca atattctgga
    15961 ggaatgcgtc aacgtatcgt tattgctatt gcccttgcct gccgacctga tgtcttgatc
    16021 tgtgatgagc caacaactgc cttggatgta actattcaag ctcagattat tgatttgcta
    16081 aaatctttac aaaacgagta tcatttcaca acaatcttta ttacccacga ccttggtgtg
    16141 gtggcaagta ttgcggataa ggtagcggtt atgtatgcag gagaaatcgt tgagtatgga
    16201 acggttgagg aagtcttcta tgaccctcgc catccatata catggagtct cttgtctagc
    16261 ttgcctcagc ttgctgatga taaaggggat ctttactcaa tcccaggaac acctccgtca
    16321 ctttatactg acctgaaagg ggatgctttt gccttgcgtt ctgactacgc aatgcagatt
    16381 gacttcgaac aaaaagctcc tcaattctca gtatcagaga cacattgggc taaaacttgg
    16441 cttcttcatg aggatgctcc aaaagtagaa aaaccagctg tgattgcaaa tctccatgat
    16501 aagattcgtg aaaaaatggg atttgcccat ctggctgact aggaggaagg aaatgtctga
    16561 aaaattagta gaaatcaaag atttagaaat ttccttcggt gaaggaagta agaagtttgt
    16621 cgcggttaaa aatgctaact tctttatcaa caagggagaa actttctcgc ttgtaggtga
    16681 gtccggtagt gggaaaacaa ctattggtcg tgctatcatc ggtctaaatg atacaagtaa
    16741 tggagatatc atttttgatg gtcaaaagat taatggtaag aaatcgcgtg aacaagctgc
    16801 ggaattgatt cgtcgaatcc agatgatttt ccaagaccct gctgcaagtt tgaatgaacg
    16861 tgcgactgtt gattatatta tttctgaagg tctttacaat caccgtttat ttaaggatga
    16921 agaagaacgt aaagagaaag ttcaaaatat tatccgtgaa gtaggtcttc ttgctgagca
    16981 cttgactcgt taccctcatg aattctcagg cggtcaacgt caacgtatcg gtattgcccg
    17041 tgccttggtc atgcaaccag actttgttat tgcagatgag ccaatttcag ccttggacgt
    17101 ttctgtacgt gcccaagtct tgaacttgct caaaaaattc caaaaagagc tcggcctgac
    17161 ctatctcttc atcgcccatg acttgtcggt tgttcgcttt atttcagatc gtatcgcagt
    17221 tatttacaag ggtgttattg tagaggttgc agaaacagaa gaattgttta acaatccaat
    17281 tcacccatat actcaagcct tgctttcagc ggtaccaatc ccagatccaa tcttggaacg
    17341 taagaaggtc ttgaaggttt acgacccaag tcaacacgac tatgagactg ataagccatc
    17401 tatggtagaa atccgtccag gtcactatgt ttgggcgaac caagccgaat tggcacgtta
    17461 tcaaaaagga ctaaactaat aatggtttta taatttccat gtcaattttt atgggaatta
    17521 taaaactttt ttgttggact gatttgaaaa actttgaaag cttcctgaaa ga
//
LOCUS       DAVBCB010000048        17282 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVBCB010000048 DAVBCB010000000
VERSION     DAVBCB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17282
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.048"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..692)
                     /locus_tag="VQU28_001593"
     CDS             complement(<1..692)
                     /locus_tag="VQU28_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2476923.1"
                     /translation="MNIILIAKPLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSYRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDG"
     gene            complement(803..1057)
                     /locus_tag="VQU28_001594"
     CDS             complement(803..1057)
                     /locus_tag="VQU28_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476924.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQV
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(1415..1948)
                     /locus_tag="VQU28_001595"
     CDS             complement(1415..1948)
                     /locus_tag="VQU28_001595"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476925.1"
                     /translation="MTIFKEQESEKVAEFLDILDNLKDLPIVYIDETGINRYLYRPYA
                     GAPRGEKVYEKISGRRFERTSIVAGQVDGEFIAPMIYKKSMTSDFFVEWFKTQLLPAL
                     KTPHVIVMGNASFHPKNILDELCIQDKHFFLPLPPYSPDLNPIEQAWAILKKKVTDLL
                     REVPTIFECLECFFKTR"
     gene            complement(1935..2261)
                     /locus_tag="VQU28_001596"
     CDS             complement(1935..2261)
                     /locus_tag="VQU28_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476926.1"
                     /translation="MAYNTDFKQGALDSIKGGHRHVEAAKVFWCWRQNSLHVGKERRE
                     QRNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIAAHFDCAVPSVWAALKQIKVTL
                     KKDDDL"
     gene            2495..4213
                     /locus_tag="VQU28_001597"
     CDS             2495..4213
                     /locus_tag="VQU28_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HET2476927.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPKEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            4324..4671
                     /locus_tag="VQU28_001598"
     CDS             4324..4671
                     /locus_tag="VQU28_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2476928.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFVHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(4949..5785)
                     /locus_tag="VQU28_001599"
     CDS             complement(4949..5785)
                     /locus_tag="VQU28_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HET2476929.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPEILKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(5798..6427)
                     /locus_tag="VQU28_001600"
     CDS             complement(5798..6427)
                     /locus_tag="VQU28_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HET2476930.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLDNLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(6437..7078)
                     /locus_tag="VQU28_001601"
     CDS             complement(6437..7078)
                     /locus_tag="VQU28_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HET2476931.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(7224..8453)
                     /locus_tag="VQU28_001602"
     CDS             complement(7224..8453)
                     /locus_tag="VQU28_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HET2476932.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEESENLLT
                     KWMIARSYQEMDDLDTAYEHYQELIGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(8443..9609)
                     /locus_tag="VQU28_001603"
     CDS             complement(8443..9609)
                     /locus_tag="VQU28_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HET2476933.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            9753..10766
                     /locus_tag="VQU28_001604"
     CDS             9753..10766
                     /locus_tag="VQU28_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HET2476934.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(10811..11230)
                     /locus_tag="VQU28_001605"
     CDS             complement(10811..11230)
                     /locus_tag="VQU28_001605"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HET2476935.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDQVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQU28_001606"
     CDS             complement(11241..12647)
                     /gene="atpD"
                     /locus_tag="VQU28_001606"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HET2476936.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFAGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(12733..13611)
                     /locus_tag="VQU28_001607"
     CDS             complement(12733..13611)
                     /locus_tag="VQU28_001607"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HET2476937.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(13627..15132)
                     /locus_tag="VQU28_001608"
     CDS             complement(13627..15132)
                     /locus_tag="VQU28_001608"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HET2476938.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALIHGFLDTVPV
                     DDIVRFEEEFHAFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(15147..15683)
                     /locus_tag="VQU28_001609"
     CDS             complement(15147..15683)
                     /locus_tag="VQU28_001609"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HET2476939.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQU28_001610"
     CDS             complement(15683..16177)
                     /gene="atpF"
                     /locus_tag="VQU28_001610"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HET2476940.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQU28_001611"
     CDS             complement(16191..16907)
                     /gene="atpB"
                     /locus_tag="VQU28_001611"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HET2476941.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(16942..17142)
                     /locus_tag="VQU28_001612"
     CDS             complement(16942..17142)
                     /locus_tag="VQU28_001612"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HET2476942.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
BASE COUNT         5222 a         3785 c         3096 g         5179 t
ORIGIN      
        1 ccgtcagtag tagtcatgct actgagtttt ttatctgtga cattgtagct ggtaccaatc
       61 agttgttttt tattggtaat gtaggttccg tcactttctt tttctccaat tccagtatca
      121 tttttcaatg atagcaaacg cccttgttca tcaacatagc gaactttcac attttctgag
      181 attagttctg ccaattctga ggtttttttc tttttcttga tttcttcggt tattttccct
      241 ttctcttctt cgggaatatt tagttttgga atgatttttt caacaacggt tcgtgatggt
      301 tccacagtat cttggatgac tgaaaagtca gctagaattg ggagattata ctgaacacgg
      361 taactttgag tgtttactcc tactctttca ttattctctg aaaatacttg tacggtataa
      421 gaaacaacat cttttcctaa tagaacatcc ccagtagaga aatagccgcc ttttcctagt
      481 ttgctatctc cagagcccac ttctttccta attttatcag atagtttttt accagtcagt
      541 acattcgttc gcacaatccc tttgtctacc cctacaaagt gggagaactt tttgaactct
      601 tcagaaccag atctagccca accattatta agggcatttg cttttgtatt tgtattctct
      661 ctcaaaggtt tggcgattag aattatattc atcggcactt agagttgctg ctatatctga
      721 ctcttgaata ccaacttcct tactaccatt tctagcggca gtatatgtgt atttatctgt
      781 ttctacttct agctgattat ccttaacatg ataggtaatt ttctcctggt ataagagtct
      841 tcagtagttt atatactctg tcatcatctt tagctttaga agcagctgag tttagccaag
      901 ttggggtatt aggagtatct ttatcaacct gtttcaagac attattcaag tcatttgcta
      961 gcctattggt tatcctcact gtgaaagttt tcttccagtc tgaaccactt ttattagact
     1021 tagatatgct catgaaatcc tggtcaacat tcaacacaat atcgccttgt tttttagcat
     1081 cttccgttgc ttctgcttca aattgatcat ggagtttttg ttgctctggt gtatagtttt
     1141 tttatttaca cgatttaatg tactattttt tattttagta ttaagaactc cattaatacg
     1201 atttttaaac tctgcttcaa aaaatccctt aatttgttgg tgcgcaagtt caacattcaa
     1261 acctttctta tacaagtctt cattctgata caagtcttca ttctgaacga tttcggctac
     1321 acggtgaatg gtttgatcgt ataccgtctt ctcttaacca gcagtatcct tagttttaat
     1381 gactactcga taggttcctt tagaaccgtt atagtcatct agttttaaaa aagcattcca
     1441 aacattcaaa aatagttgga acttccctta ataaatccgt cactttcttt ttcaagatag
     1501 cccaagcttg ctcaatagga ttcaaatccg gtgaataagg tggtagaggt aagaaaaagt
     1561 gtttatcttg gatgcagagt tcatccaaaa tgttcttggg atgaaaacta gcattgccca
     1621 tgacaataac atgaggtgtc ttcaaagcag gtaggagttg cgttttgaac cactccacaa
     1681 agaaatcgct tgtcatgctt ttcttgtaaa tcatgggagc tataaactct ccgtctactt
     1741 gtcctgcaac aattgaagtt cgctcaaaac gacgtccgct aatcttttca tagactttct
     1801 cccctctagg agcccctgca taaggacgat agaggtagcg gttgattcct gtttcatcaa
     1861 tatatacgat tggtaaatct tttaggttat ccaaaatatc aagaaactca gcgactttct
     1921 ctgactcttg ttccttaaag atcgtcatct ttttttaaag tgaccttaat ctgctttaaa
     1981 gctgcccata ctgaaggaac agcacaatca aaatgtgccg caatttcccg taaaaaagca
     2041 tctggatgag cctctacaaa ggctttcaat tcttctaaag ggatcttacg ctttttgacg
     2101 actcgctttt tccgctctaa gttcctttgt tcacgtcttt cttttcccac gtgaagagag
     2161 ttctgacgcc aacaccaaaa aactttggcc gcctcgacat gtctgtgccc tcctttgatg
     2221 gaatctaatg ctccttgttt aaaatcagta ttatatgcca tagctttatt agaccatgct
     2281 tattttaaac taagcaacta tatcaatgtt ttgttatgag aagacttact ttttttactg
     2341 aaattgaatt tttcctagcc tcaacatcat ataaccccaa aattcaatct gacctggttt
     2401 gaagagatgt tcgaagcaga taaggtttta ctaaaactaa aaaaaatgat ataatcaaac
     2461 tgattaaatg ttttaaaaat aaaggagaat ttgaatgtct taccaagaaa attaccagaa
     2521 atgggttgat tttgtggagc ttcctgacta ccttcgtcaa gatttggaaa atatggacga
     2581 aaaaactaag gaagatgcct tctatacaaa tcttgaattt ggtactgcag gtatgcgtgg
     2641 cttggttggt gctggtacaa accgtatcaa catctacgtt gttcgccaag ctactgaagg
     2701 attggctcgt ttgattgagt caaaaggtgg aaacgagaaa gaacgcggtg tagcaattgc
     2761 ctacgatagc cgtcacttct cacctgagtt tgcctttgaa tctgcggcag ttcttgctaa
     2821 acacggcatc aaatcttacg tatttgaaag ccttcgtccg actccagaac tatcatttgc
     2881 agttcgtcat ctcaactgtt tcgcaggtat catggtcaca gccagccaca accctgctcc
     2941 atttaacggt tacaaggttt acggtgaaga cggtggacaa atgcctccac acgatgcgga
     3001 cgctttgact acttatatcc gtgcaatcga aaacccattt gcagttgaag ttgctgatgt
     3061 ggaaactgaa aaagcttctg gcttgattga agttatcggc gaagctgttg acgtagaata
     3121 ccttaaagag gttaaggacg taaacatcaa cccagccttg attgaagaat ttggtaaaga
     3181 catgaagatt gtctacacac cacttcatgg tactggtgaa atgttggctc gtcgtgctct
     3241 tgcccaagca ggatttgact ctgttcaagt tgttgaagcg caagcaactg ctgacccaga
     3301 cttctcaact gtaacatctc caaacccaga aagccaagca gcctttgccc ttgctgaaga
     3361 acttggtcgt caagttggtg cagatgttct tgtcgcaact gacccagacg ctgaccgtgt
     3421 tggtgttgaa gttcttcaaa aagatggtag ctacctcaac ctttcaggta accaaatcgg
     3481 tgctatcatg gctaaataca tcttggaagc tcacaaaaac gctggaactc ttcctgaaaa
     3541 tgccgctctc tgcaaatcta tcgtttcaac tgacttggta acgaagattg ctgaaagcta
     3601 cggcgcaact atgttcaacg tcttgacagg tttcaaattt atcgctgaaa aaatccaaga
     3661 attcgaagaa aaacacaacc acacttacat gatgggattt gaagaaagct tcggttactt
     3721 gattaaacca tttgtacgtg ataaagatgc catccaagcc gttcttgtcg ttgctgaact
     3781 tgctgcctac taccgttctc gtggtttgac acttgctgac ggtatcgaag aaatctataa
     3841 agaatatggc tactacgcag aaaaaacaat ctctgttact ctttcaggtg tcgatggtgc
     3901 tgaacaaatc aaagcgatta tggctaaatt ccgcaacaat gctccaaaag aatggaacgc
     3961 aacagctatc actgtcgtag aagacttcaa ggcacaaact gctactgttg ctgacggtac
     4021 tgttacaaac ttgacaactc ctccaagtga tgtgttgaaa tacacacttg ctgacggttc
     4081 atggattgcc gttcgccctt caggtacaga accaaaaatc aagttctaca ttgcagttgt
     4141 aggagaaacc aacgaagaat cacaagctaa gattgctaac atcgaagcag aaatcaatgc
     4201 atttgtaaaa taaagaccta taaaagatga gaccaaccaa cctcatcttt tttcgtatca
     4261 aatctaagca attttagaaa ctttatggca tactagactt atcaatgaaa gcgaggtaat
     4321 ctcatggaac aatttttaga taacatcaaa gaccttgaag tcactacagt tgtgcgtgcg
     4381 caagaagctc ttgataaaaa agaaactgca accttcttta tcggtcgcaa aacttgccct
     4441 tactgccgta aatttgcagg tacattgtca ggtgtcgtag ctgaaaccaa agctcacatt
     4501 tacttcatca atagtgaaga accaagccaa ctcaatgatt tgcaagcatt ccgctcacgc
     4561 tatggaatcc caactgtacc aggttttgtt catattacag atggacaaat caacgtccgt
     4621 tgcgactctt caatgtcagc acaagaaatc aaagatttcg caggattgta aaaaatcagc
     4681 taacaagtct taaaatcaca aaaacgcata atgtaaggtg caaaaacctt gatattatgc
     4741 gttttattgt tcctaaaaca gttacatcag catttcatta cagaacattt taattttgga
     4801 agtcaaatca atttttaaca atgttttaga aatcacagtg ggctattcca tattaaatta
     4861 tccagattca atcgctatta tcgttttcct agattacaaa aaaactgcgt tttgctaatc
     4921 aaaacgtagt ctcagccact attttatctt actgtccttc ttttacttct ttggttgcta
     4981 catcttctcc aaaccatttt tggctgattt cttggaactt gccgtccttg taaagactag
     5041 aaaaagcttc atttatcttc ttaaccaagg ttgtatcttc cttacgggct ccaaccgcaa
     5101 aagcttctgt ttctagtcca actgtaaaga cattataatc gtttaaaaca ccttctgctt
     5161 ctaaataata gtttgcatag acacggtcaa tcaatagacc atcaattcga tcgtttttca
     5221 aatcaatcaa ggcttcatta aaggtttggt attgattcgc ttccttatta gcgacaatat
     5281 tcttcaaaat ttctggattt gcttcaaagt ccgcataacc agatgaacca gcttgagctc
     5341 ctaatgtctt tccagtcata tcctttgcag tcgtgatacc agatgatttc ttcgtaacca
     5401 atacctgctc attcttcata tatgagttac tgaaagccac cttttcacgg cgttcgtctg
     5461 tagcggaata gccattccaa atcagatcaa tcgttccttt tgtcaattca gcttctttca
     5521 aatcccaatc aatcggttgc caatttaccg tgattccgta tttttcaaaa acagctgtag
     5581 ctaaatcaat atcaaatcct gcataagaac catctttctg agcaaatccc attggaacaa
     5641 aagtactatc aaatccaata gtaatagact tgttagactg gtactttgac caattatctc
     5701 cactagtttc gctagaattt ttcccacaag ctactaagaa caaagcagtc atcagactga
     5761 ctaatacaag catccatttt ttcatccatt tttcctccta tttaggttct actttcaata
     5821 atacatctgc gatattttca gcaaactgca aatcatgggt aaccacaatc tgggtcatcc
     5881 caagttccct attttgcaag attagcttct ccacttccaa acgtaattct ggatccaggg
     5941 cagaagttgg ttcatcgtag ccaatgattt ctgggtcaat catcatagca cgcgccaaag
     6001 ccacccgctg cttttgccca ccagatagtg agaaaggata ggcctctgcg tgtcctccta
     6061 gtcctaactg ttccaagagt ccactcgcct tcttctcagc ctcttcctgc ttcattccca
     6121 tggtcttcac aggcgataag gtcaaattgt ccagaactga taaatgaggg aacagttgaa
     6181 aatcttggaa tacaaatccc agtagattgc gcttctgcaa ttcatccagc tctaaaggtt
     6241 gtccattata aaagatttgc cctgaatcaa tggtttcaag acctgcaagc atacgtaaaa
     6301 gagttgtctt acctccacca gaaggtccaa cgatagccag gatttgcttt tcaggaatac
     6361 ttagactgaa attagacagg atttgtttgt ctccaaagac tttattgata tttcgtaatt
     6421 ctaacatggc agcctcctat ctataataac tatacttctt ctcaactttt ttggaaataa
     6481 ttgtcacaat cccaatcaaa atcaaataaa tggctcctgc caagaacata ggaactagac
     6541 tagcatcgcg gttagcagct gttcgactag ccaagataag gtctgaaatt ccgagagcat
     6601 agaccaaaga agtatccttg accaaactca taacttcatt aaagacacta ggaagaacga
     6661 tcttggtcac ttggggcaag ataatatagc gcactctgtc aaaagggcta aacttcaaga
     6721 ccttggcggc ctcatactgt cctcttggaa tagtgtcaat tcccccacgg aaaatttctg
     6781 caaagtaagc tgcatagttg agaacaaagg caataatagc tgcaggaagg cggtctaaac
     6841 gaatcccaat acttgggagc acataataga taaaaatcag ttgcaagagt aagggggttc
     6901 ctcgcataac ccagatgtaa atgttaatca gataatggag gggcttccaa tggacttgca
     6961 aggcaaaggc aatcaaaacg cccaagggaa tcgaaaatag caagaccagt gcaaagacct
     7021 gtacagtcgt gctcgcacca ttcagtaaac tcggtaatat ctcaaataaa taagacatac
     7081 tgcacctcct aaaagtaatt gttcctatta tagcatgaat aaacaagatt accaagtatt
     7141 tttgtaaaaa gattctacca tatacaaata aaaaccgtag ctatcaaaag agataaacta
     7201 cagttctata tgtttaaaca ttcttacaat ctctcaaaca gttcttgcat ttgcacatca
     7261 tctggaacca gttttaagta agtgtgagca tggacttttg cttcttcaaa atgtcccaat
     7321 tcacgcaaga gatagatata gtgttccaga aattctggat tgtccttcaa atctcctatc
     7381 aactcttgat aatgctcata agcagtatcc aaatcgtcca tttcttgata agaacgagca
     7441 atcatccact tggtcaaaag attttctgac tcctcactct gcaagtctag aatatcctca
     7501 taacgctcct gctccagata aatagtggct aaacgaagca agatttcttc tgtatcctca
     7561 gcgtcttctt ttgcagtaag gagataattt tctgcaccac tagcatcatg caattcataa
     7621 gaaaattgtg aagcagctag caagaggcga gtttcaaagg gatttttctc taatccttgc
     7681 ttagcgatac gcagggcttc ttgaacttga tgttccttat gtaaagcctg actgtaccca
     7741 tactcatagc cttcaaagtc aggagaaatg gtatcaagct gcttaaagta gagggtggct
     7801 ttttgatatt cttcttgatc aaaataaaga ctggccaact caaaagctgt taagtcatcg
     7861 tattctaact ccagggcttt ttctaaaaac tcagtagccg tttcaaattt ccctaactga
     7921 gcataggcaa agccaattcg ttgataggtg gaaatgcccg tttgctcata aatcgagcga
     7981 ttatctaact gggcataggc ttgaatagcc gcttggtaat tttccaactc actatccaac
     8041 tctgccaaac ccaatatcaa gagagaatcc tctgagtagg tcaaggcctc caataatttc
     8101 tcacgtgcca catctgtcaa accttccagc tggtaaaggt ctgccttcag agccaaagac
     8161 gagacatacc agtcactgtc agcttggatt tcctcaagat aggtaaaggc ttcttctatt
     8221 tgaccatcct cgctagcaat tgcagctaga ttaagatgaa cctctggaaa atcctctaca
     8281 attttcaggt aaatttcctt ggcctgagga tagaaaccaa tcccttcaag ataagttgcc
     8341 aactcataca gaagatcact tgaatcattt tctaaagctt tggcgaaata atgctcagcc
     8401 ttagttaaat cttgctcctc caaagcctgt aacatctgtt gactattgtt cactcttgac
     8461 ttcctcaccc tctaattcat atagaccact gactacctta taccattcga aaatggctga
     8521 aatgacaacc ttagcagagg cataaaccgg aataccaagt aaaactcccc agataccaaa
     8581 catagatcct gaagttaaca aaacaaagag aacattaata gggtggatgt ttaattgact
     8641 tcccaaaatc aatggagaga caaaacggcc ttcaatagtt tgttctacga taaagacaat
     8701 cactactttc aaaagcatga ctggaccagc aatcaaaccc aatactagag caggaagcat
     8761 ggctagaaag ctaccaagat aagggaccag atttaaaata ccagcagtaa cccccagcgt
     8821 aaccgcatag cgtagaccaa taatcttgaa gaagatgata aacattactg ctacaataat
     8881 agccactgtc acttgccctc gaacatagtt ggacaactgt tgattcacat ctgataaaac
     8941 ttgtccaaca ggttccttca attttcttgg aatgaattgg gtcaaatagt tacgcaagcc
     9001 tttcccatca cgcaagagat aaaagagcat gaaaggaacg ataatcaagg caacaatcac
     9061 ttgagaagct ccgctaataa aggcactcac ccagttgact gcctgagatg aaaccttact
     9121 tgccaaaact gtagcctggc tagaaaaatt ggtcaaaact tgctctaatt gaggtctgaa
     9181 atcatctggc aggtgctggg ctaccaatcc attaacaatc ctatctatat cttctaagta
     9241 aacaggaacg tttcttgcaa aggtcaaaac ctgacgttgc agatttggaa tggcgactgc
     9301 caagccccaa atgataaaga gagcgatgat aacaaagaca atagtgatag ctataacacg
     9361 attaacctta tgcttctcca tccaatcaac aataggattc aacaaataat ataacaaacc
     9421 agacaaaatg actggcaaca tcacaactgc taaaaagtct aaaacaggtg aaaatagaaa
     9481 actaatctta cttaaaataa aaagattcag tcccaataat aaggttacta aaaataccgt
     9541 aattgccttg ttatctaaaa accacttgaa aaaccaagat aggctaaaat gtttctcttt
     9601 ttgctccata aatacttcct ttctattgtt ctctcattat accatttttg aaacaaaata
     9661 actctttttt aaagtgctta catctaattg gatatcttta atttacttgt ttttgtgata
     9721 taatagtctt atctttacac agaggtatag taatgaaaga aactgtttat tttggaactt
     9781 atacacgtcg tacttctcaa gggatttaca aggcagactt tgatacagaa actggtcagc
     9841 tttcaaatct agaacttttt gcagctgagc caagtccaac ctaccttgcc tttgaccagc
     9901 accaacattt atacactgtt ggtagccaag acgataaggg gggaattgca gcctatcaaa
     9961 ctgacgggac tgtgttaaat catgttgttg aagaaggagc tccccactgt tatgttgctg
    10021 tcgatgaaaa gcgtgatttg gtttacgcag ccaactatca caagggacaa gtccttgttt
    10081 ataaacgcca ggaagatggt agtcttctac ttagtgatat ggatcaacac agtggccaag
    10141 gtccacatga aaatcaagct tccccccatg ttcactatac agatttaaca cctgaccact
    10201 atctagtgac ctgcgacttg ggaactgacc aagtcatcac ctatgacctc gatcaagaag
    10261 gaaaattatc taagctctat acctatcaca gcaagccagg agcaggctca cgccatatca
    10321 ttttccataa ccactataaa atcgcttatc tcatttgtga actcaatagt actatcgaag
    10381 ttttaatcta cgatggcgtt ggcgaatttg aacgtatgca ggtcatttca actttaccgg
    10441 aagcttacga aggctttaat ggaaccgctg ctattcatct ctctaaagac ggtaaatacc
    10501 tctacgcttc taaccgtggc catgattcta tcgcagtata taccatcctt gcggacggta
    10561 gcttagagtt attagaaatc gttccaacgc atggtcagac tccacgtgat tttgatttga
    10621 cacccgacca aaaatttctc attgttgtcc atcaagactc tgacaatgca actgtcttta
    10681 aacgtaattg tgacaatggt cgtctagcag aactctccaa cgacttccat gttcccgaag
    10741 cagtctgcat ccgttttgct ccttaaaaca ccataaaaaa tgaacttggt attttcaagt
    10801 tcattttttc ttatagtcta tttccgacat taatacggtt aatagcacgt tgcaaagcaa
    10861 tcttagcacg acgttcttgg tcaatcaaat gtttgtcttg tgcttcttca attgcacgtt
    10921 ctgcacgaag tttggcacgt tctgcacgac tgatatcgat atcacgagca cgttctgcag
    10981 agtcagcgac gattgtgatc atatcattgg caatttcaat aacaccaccg tttactgcaa
    11041 tccagttcac ttgatcttta tcatcgatac gttttacctt tacttcatca actgctaaaa
    11101 ccgcaatcat attttcatgt cgtggcaaga tccccatctc accatccaga gttcgaaccg
    11161 atacatagct ggcatggtga tcatagacga gaccatctgg tgtcacgatc tggacagtta
    11221 actgagccat agttcacctc ttaaaatccc attttttcag cttttgcaat cacatcttcg
    11281 atagaaccta caccacggaa ggcatcttct ggcaagtggt cgtatttacc atcaaggatt
    11341 tccttaaagc cacgtacagt ttcagcaact ggaacataag aacctggctg accagtaaat
    11401 tgttccgcaa cattgaagtt ttgtgacaag aagaactgga tacgacgggc gcgagcaacc
    11461 aaggtctttt cttcatcaga aagttcatcc ataccaagga tagcaatgat atcttgcaat
    11521 tcatggtaac gttgaaggac acgttttact tcagcagcaa ctgcatagtg ctcttctcca
    11581 acgatttcag gtgccaaggc acgtgagctt gaagcaagtg ggtcaacggc tgggtagata
    11641 cccaattgta ccaacttacg ttccaagttt gttgttgaat ccaagtgagc gaaggctgtt
    11701 gctggcgctg ggtcagtata gtcatccgct ggcacataga tagcctggat agaggttaca
    11761 gaacccttct tggttgatgt gatacgttct tgcaattgac ccatttccgt agcaagtgtt
    11821 ggttggtaac caacggctga tggcatacga cccaaaaggg cagatacttc tgaaccagcc
    11881 tgagtgaaac ggaagatatt atcgataaag agaagcacgt cttggccttc cacatcacgg
    11941 aagtattcag cgattgtcaa accagtaagg gcaacacgca tacgtgctcc tggtggctca
    12001 ttcatctgac caaagaccat ggctgttttc tcgataacgc ctgattcttt catttcccag
    12061 taaaggtcat tcccctcacg agtacgttcc ccaacaccag caaatactga aataccaccg
    12121 tgctcttggg caatgttgtg aatcaattct tggattaaga cagttttacc aactccggca
    12181 ccaccgaaaa gtccaacttt accaccttta aggtaagggg caagaaggtc aataaccttg
    12241 atccctgttt caaggatttc agaagaggta gacaactcat caaaagttgg agctttttta
    12301 tgaattggct gacgctctgc gtcttctgta aaaggagctt ccaagtcaat ggtatctccc
    12361 aaaacgttga agacacgtcc caaagtttct ttacctactg gtacagagat tggacgacct
    12421 gtgtccaata cttccattcc acgagtcaac ccatctgttg attccatggc gatagtacga
    12481 accataccat ctcctaactc caaggctact tcaaggacga tttttgtttt tctttcgtca
    12541 tttttgtaga cgacaagtgc attgttaatc tcaggaagtt tttcccctgc tgcaaacaaa
    12601 acgtctacaa cgggaccgat aacctgagca attttacctg aactcatctc cttctcctat
    12661 tctatataag atactgtcgg ttcctagctc aactaggtcc taagttcatt ttcatacgag
    12721 ctggactaga gcctattcta aggcactagc acctgctacg atttctgtaa tttcttgtgt
    12781 aatcgccgcc tgtctggcac ggttatactg aattgtcaaa tcattgatga ctttcttagc
    12841 attatctgtc gctgtttgca tggctgtcat acccgcagca ttctcagctg tcttggcatc
    12901 tataatggca ccgtaaatca tactttctgc aaactgaggc aacaactgct ccagaatttc
    12961 ttctcggctg gtttccaatt caaaagtcaa gctgtactct tcatccgctt catttggatc
    13021 caagtcaaca atcggaagca tttgttccac acgcatttga ctggttagcg tattgacatg
    13081 gtggttgtag caaacataaa gctcatcaaa gagttcattt tggtacattt caacagtttt
    13141 tgaaataatc ttacgaactt gatcaaagct aggttggtct gccaagccac gtaattcata
    13201 aagtggttga ataccgcgag ccttaaagaa atcagctccc atcccaccga tacagatcat
    13261 ttcaaaacct ttaccgtctg ggtggtattc ttctttcaac tccataacag ctttcaaaat
    13321 agaggaatta taacctccaa ccaaaccgcg gtctgaagtg ataacgatat agcctgtctt
    13381 cttcacagaa cggctaatca acatcggatt agttgaagca ccagctccat taccatgaag
    13441 gatatctgtc aaaagtttac gcactttctg agcgtaaact tggaagttgc gagcagcttc
    13501 ttcagagcga cctagcttag cagccgatac catttgcatg gcattagtga tttgactcgt
    13561 attttttgtt gaggcgattt ttgttttaat atcatttaga gatactgcca tctgacacct
    13621 ctattcttat tggaagctag attgattgag aaactctgta atcgcagcat ccaagactgc
    13681 ttcttctggc aagtcttttg tatcacgaat ggtttccaaa atctctggat gttgagcatc
    13741 aaagaaggca tggaactctt cctcgaaacg aacaatatca tctactggaa cagtatccaa
    13801 gaaaccatgt atcaaagcat aaagaatggt tacttgtttc tcaacaggta atggtttgtg
    13861 aacaggttgt ttcaagacct caacggtacg acgtccacgg ttcaacttag cctgtgttgc
    13921 tgcgtccaag tcagaaccaa acttagtaaa ggcttccaac tcacggtatg aagcaaggtc
    13981 gatacgaagt gtaccagcaa ccttcttcat ggctttgatt tgtgcagaac cacctacacg
    14041 agatacagat gaacccgcat cgatggctgg acgaatacct gcattgaaga ggccatcgcc
    14101 aaggaagatt tgtccatcag tgatagaaat cacgttggtt gcgatatagg ctgagatatc
    14161 tcctgcttgt gtctcgataa atggtagggc tgtaattgat ccaccaccaa gttcatcaga
    14221 aactttagct gagcgctcaa gcaaacggct gtggagatag aaaacatccc ctgggaaggc
    14281 ttcacgacct ggaggacgac gaagcaagag cgacagttca cgataagcta ccgcttgttt
    14341 tgatagatca tcgtatacaa tcaaaacatg cttaccttga tacataaatt cttccgccat
    14401 agcgacccca gtataaggag ctaggaagag caatggagat ggttgtgaag cagaggctgt
    14461 cacaacgatt gtgtagtcca aggcaccgta ctgacgaagt gtttctactt gcgtacgaac
    14521 tgttgattct ttttgtccaa tcgcgacgta gatacagatc atatcttgat ctttttggtt
    14581 caagattgta tcaatcgcaa tggttgtttt ccctgtctga cggtcaccga taatcaactc
    14641 acgttgacca cgaccaatcg gtacaagggc gtcaatagct ttcaaaccag tttgcaatgg
    14701 ttctgaaaca gacttacgtt gcataacacc aggagctggt gcttctactg gacgagtttt
    14761 atcagtgtgg atttctccaa gaccgtcaac tggacgacca agcggatcca caacacgacc
    14821 aatcagactt tcacctacag ggacttccat gattttccct gtacggcgga ttgtatcgcc
    14881 ttcacggata tctgtaaagt cacctaggat gataatacca acgtctgttg actccaagtt
    14941 ttgagccata ccataagagc cgttttcaaa attcaacaac tctccactca tgacattttc
    15001 aaggccgtga gcacgcgcga taccgtcccc gatataggtt acaacacctg tttcagtcac
    15061 atcaaaattg ggtttgaaat tttcaatttg ttgcttaatt aaagcgctga tttcttgtgc
    15121 gttaattgcc aaaagaacac cactttctat ttcaaatttt ctttaacaac tttaagttgt
    15181 tgtttaatac tcacatcaat tgtcttgtga ttggcaaaaa tgacaaaacc accaatgaga
    15241 ctttcatcga tttgttcttt tacactcctt actttcagag acattttttt ctcaatcaaa
    15301 gggagcaaac gagtcttctg ttcatcagtt agaggatgag cagacgtaat cgtcacttca
    15361 aatcgatttg tttctttttc aagtcggttc aagcaatcta caagcacatc ataaaaaaga
    15421 tttgctctgt gattgtaggc cagaacctgg ataaagtttt gtaataaagg cgacacagaa
    15481 tcttggaaaa aagcaattgt tttttcctta tccgactcgt ctactgccac ttgttttaaa
    15541 aaagaaggca gacctgtttt ttcaacaact tgcttgattt gagtcaagtc tgaaaagata
    15601 cggtcttctt ctcctttttc aagtaccaat tggacaaaag gcatgctgta tttttcaatt
    15661 acctttactg ttttcttgtc cattaagctt ctcctagctg atcgatatac tgatcaatga
    15721 gtgctttatg ggcatgactg tcaaggtttt gtgagatgat tttaccagct aagctgatgg
    15781 tcagatctgc gacctcaccc ttaacactct gtaaagcttc tactttattt tgagcaattt
    15841 cttgattggc tttttctttt aagtgtcctg cttctagttt agcatctgct aagatattag
    15901 ccttactttg ctcagctgtt tcctttgcat tttcaatgat tgtcttagct tctttacggc
    15961 taccagccaa ttcatcttcg cgtttttgag ccaatacttc tgctttttga cgggcttctt
    16021 cagctctgtc aatatctgaa gcaatttttt cagctctttc ttcgaaaatg cctgtaatat
    16081 tagaccatgc aaatttttta atcaagacta gcaaaagaat aaaagagcca gtgattaaaa
    16141 taaaattacc aattaattca cctactgtta cgtgcatcag ttactccttt ctattcctct
    16201 tcaatattaa tcttattccc taaatacaca gatgtcaaaa gagtaaaaac ataagcttgg
    16261 atgcaggaaa taaagacaga aaatgcagtc caagccaaat tagctccaaa ggctactgga
    16321 taccaataaa tagcttggtg ggaaagaaga agtaacaaac tcgtcatgac ctctcctgca
    16381 aagatattcc caaaaatccg caaagccaaa gataagaagt ttgtaaattc ttccaagata
    16441 ttcatcggta tgacaaaaac aggactcata aaagatttta tacttttttt aaatccacga
    16501 cgacgaacgc tttctatatg tgtcaacaaa atgacaagaa aagataaggt taagtcatac
    16561 tgtaaattag cggttggcga actccaccag ttagtcccat cgatcgtttg aagctttgtc
    16621 attaagccaa ggttattggc aatcaccatg aaaaggaata aacaaaggaa aaagagtgaa
    16681 taatctttca tgtagcgcga accaatgtta ggttctgtaa atccaataac aaagtcatag
    16741 acatactcaa gtacattttg ctttcctttg ggtttcaagg tcatattgcg gcttgcccaa
    16801 taaataaaga caaaaataac tcccacaatc aacaaagtca tggctaacat agtcagattg
    16861 aagataacag gaccaataga gatgattgga ttaatacttt cttccatcta aaatcctccc
    16921 ttttcttctc gttccatgta tttatttgat aataaatgag aagacaagag ttacaaagaa
    16981 agttccttca ataaaggcaa cacctaaaaa catcaaacta cgaaactcag aaagcatatc
    17041 tggttggcgt gctactgatt taaacagtcc attcatcaat aaaccttcac cgacagatac
    17101 gcccatacag gcaatacata agcctaaaaa tgttaaattc ataacaaatt ctcctttaaa
    17161 ataaaattta cttacttagt gtagtcctaa aatttaaatt tgtcaacttt taaccgctat
    17221 tggaagcgtt taataaaaat tatttccatt ttgctaaatt tcaagttcaa gttagccatc
    17281 ca
//
LOCUS       DAVBCB010000049        16678 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVBCB010000049 DAVBCB010000000
VERSION     DAVBCB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16678
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.049"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..447)
                     /locus_tag="VQU28_001613"
     CDS             complement(<1..447)
                     /locus_tag="VQU28_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2476943.1"
                     /translation="MTIEPGQRIALVGQSGSGKSTLSKILSGLYKIDTGKILFDGVNI
                     NQIDKKILSQNLGVVPQDSFLLNRSILDNITLKNEVTSQKIEEVCKAVQIYDEIMAMP
                     MKFNTIISEMGSNISGGQRQRIALARALINNPSIVILDEATSALDTI"
     gene            complement(616..1929)
                     /locus_tag="VQU28_001614"
     CDS             complement(616..1929)
                     /locus_tag="VQU28_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000197307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2476944.1"
                     /translation="MTRKVPNIEQMSQIECGLCCCLSILHFYKSKETLLDLRRDIEKG
                     RDGYSIGDLKQLLNKRNFDTGSYQVKDVNKISELPLPLIAFWDNQHYVVIYKVKKNKV
                     YIMDPSKGYINYEFKEFSKHFSNIVLLSFPNENYQSLKSQFPSPWIRVFSSFSKVKGR
                     LILTLLFSIISYLIILSVPVMTSKFINSALGNTFSFQTSFLILFSLLCLYLISILARS
                     MGILFSNIFFSRDIESFTFKHLLKLPYSFFELRAKGDILYRISSLSGFRELFTNQVVG
                     GVVDIGTILSVVIYMFLSSKTLSIIALILSLINFLFLFSTRKIMYDTVNRELQEQSLI
                     YSVETEALNTISSIKISGLEDEIYENWSKYLKNVLTKYKKRSIVHILYNSATNVFQLF
                     APIIILIFGLDNVLNGKILLGEVVAFQTMASILFSSEISIFNAYT"
     gene            complement(1922..2470)
                     /locus_tag="VQU28_001615"
                     /pseudo
     CDS             complement(1922..2470)
                     /locus_tag="VQU28_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000059560.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2551..2613)
                     /locus_tag="VQU28_001616"
     CDS             complement(2551..2613)
                     /locus_tag="VQU28_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HET2476945.1"
                     /translation="MKKISKFLPILVLVMDIIII"
     gene            complement(2782..3770)
                     /locus_tag="VQU28_001617"
                     /pseudo
     CDS             complement(2782..3770)
                     /locus_tag="VQU28_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050775.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3719..4846)
                     /locus_tag="VQU28_001618"
                     /pseudo
     CDS             complement(3719..4846)
                     /locus_tag="VQU28_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423473.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(5009..5893)
                     /locus_tag="VQU28_001619"
     CDS             complement(5009..5893)
                     /locus_tag="VQU28_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HET2476946.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(6005..6859)
                     /locus_tag="VQU28_001620"
     CDS             complement(6005..6859)
                     /locus_tag="VQU28_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HET2476947.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNLTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(6917..7675)
                     /locus_tag="VQU28_001621"
     CDS             complement(6917..7675)
                     /locus_tag="VQU28_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HET2476948.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYALS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            complement(7737..7925)
                     /locus_tag="VQU28_001622"
     CDS             complement(7737..7925)
                     /locus_tag="VQU28_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HET2476949.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(8247..8813)
                     /locus_tag="VQU28_001623"
     CDS             complement(8247..8813)
                     /locus_tag="VQU28_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476950.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKTYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(9081..10760)
                     /locus_tag="VQU28_001624"
     CDS             complement(9081..10760)
                     /locus_tag="VQU28_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HET2476951.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGRIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCNHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKVDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIGLDNDEQVECVSLLEKRS"
     gene            complement(10852..11013)
                     /locus_tag="VQU28_001625"
     CDS             complement(10852..11013)
                     /locus_tag="VQU28_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476952.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(11697..12011)
                     /locus_tag="VQU28_001626"
     CDS             complement(11697..12011)
                     /locus_tag="VQU28_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001835032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476953.1"
                     /translation="MGITWKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEYKNLK
                     EKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNYDVGE
                     SL"
     gene            complement(11977..12366)
                     /locus_tag="VQU28_001627"
     CDS             complement(11977..12366)
                     /locus_tag="VQU28_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2476954.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYRDR"
     gene            complement(12754..13548)
                     /locus_tag="VQU28_001628"
     CDS             complement(12754..13548)
                     /locus_tag="VQU28_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2476955.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHVSS"
     gene            complement(13548..14555)
                     /locus_tag="VQU28_001629"
     CDS             complement(13548..14555)
                     /locus_tag="VQU28_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2476956.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(14555..15562)
                     /locus_tag="VQU28_001630"
     CDS             complement(14555..15562)
                     /locus_tag="VQU28_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HET2476957.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLATFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(15552..16577)
                     /locus_tag="VQU28_001631"
     CDS             complement(15552..16577)
                     /locus_tag="VQU28_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HET2476958.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVISDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
BASE COUNT         5225 a         3145 c         2257 g         6051 t
ORIGIN      
        1 aatagtgtct aatgcactag ttgcttcatc taaaattaca atactaggat tatttattaa
       61 tgcacgtgcc agtgctatcc gttgcctttg cccacctgaa atatttgacc ccatctctga
      121 gatgatagta ttaaatttca tcggcatagc catgatttca tcatagattt gaactgcttt
      181 acaaacttcc tctatctttt gtgaagtaac ttcgttcttt aaagttatat tatcaagaat
      241 acttcggttc aataaaaatg aatcctgtgg aactactcct aaattttgac ttaatatttt
      301 tttatctatt tgattaatat ttacaccatc aaataaaatt tttcctgtat caatcttata
      361 taatcctgat agtatttttg ataacgtact ttttcctgac cccgattgac cgactagtgc
      421 aattctctgt cctggctcaa tagtcaaatt taaattttct attacaggag ctgagtcttt
      481 ggaataagag aaagataggt ccttaatatc tattctacct tctaaagaac actttttaag
      541 accattttca acattctctt cattttcaag ccagatatca tttactctat ttaagtatcc
      601 tgctgccaaa atatactacg tataagcatt aaatattgag atttctgaac taaataaaat
      661 tgatgccatc gtttgaaatg cgacaacctc acccaataaa atttttccat ttaaaacatt
      721 atcaagtcca aaaatcaata taattatagg agcgaacaat tgaaatacat tagtagctga
      781 attgtataaa atatgcacaa tacttcgttt tttatacttt gtcaatacgt tttttaaata
      841 cttagaccag ttttcatata tttcatcttc taatcccgat atttttattg aagaaatagt
      901 atttaatgct tctgtttcta ctgagtaaat caatgattgt tcttgtaatt ctctattaac
      961 cgtatcatac attatttttc tagtactaaa cagaaacaaa aaattgatta aactcaatat
     1021 aagagctata attgataatg tttttgaact aagaaacata tatattacaa cacttagaat
     1081 agtcccgata tctactactc ccccaacaac ttggttagta aatagctccc taaaaccact
     1141 aagactagaa attctgtata aaatatctcc ttttgccctg agttcaaaaa aactatatgg
     1201 tagtttaagc aaatgcttaa atgtaaaact ttcaatatct ctactaaaaa atatattaga
     1261 aaatagaatt cccatactcc tagccaagat agaaataagg tacaaacaaa gtagagaaaa
     1321 taaaattaaa aacgaggttt gaaaagaaaa tgtattccct aatgcagaat taataaactt
     1381 tgaagtcatt actggtacgg aaagtattat taagtaactt attattgaaa atagtaacgt
     1441 caaaatcaat cgacctttta cctttgaaaa actagagaat actcttatcc atggagaagg
     1501 aaactgcgac ttcaaagatt gataattttc gttaggaaat gatagcagta ctatattact
     1561 aaaatgtttt gaaaactcct taaactcata atttatatat cctttagaag gatccattat
     1621 atagacctta ttctttttta ctttgtaaat aactacataa tgttgattat cccagaatgc
     1681 tattaatggt aacggaagtt cactaatttt atttacatct ttaacttgat atgagccagt
     1741 atcaaaattt cgtttattca ataactgttt caagtcacct atactataac catctctccc
     1801 tttttctata tcacgtctta aatctagtaa tgtctctttg cttttataga aatgtagtat
     1861 ggacaagcag caacataaac cacattctat ttgagacatt tgctcaatat taggtacttt
     1921 tctagtcatc atgtcctctt aactccagta ataaaacatt aagtaatcta tcatcttgtt
     1981 ttcgagatat ttcatagaca actccagata ctcctagcat aaaagaaaat gtattcatag
     2041 tattcaaatc cgtacttaat tctggtttga taacctgaga aacgacatta tcaaaataaa
     2101 aatctataga ttcttttatt aataggtttt cttcatcttt caatattgca tcaagacaac
     2161 tgatagtacc tacagaaccg tgacataaat tcaattctct tattttttct acattagtta
     2221 atacattttc aattattgat aaatcattat tcccaatatt agttttatcg aaaatttctt
     2281 ttctagctaa taaaatgccc ggagctccat aacaccaaaa attaggaaag gaatcattat
     2341 gatttcttca tatgtctttc caatttttta atatattatt gaaacattta tcttctttat
     2401 atagtaattc ctctatacat tttaatatat ctgtactagg attaatttca taataatatg
     2461 ataattacat tgctactcca gaataatcgt gtgctaaacc tgttactgtt atattatcta
     2521 attgtgaaat acaataatta atacactttt ttaaatgatg ataatatcca tcaccaaaac
     2581 caaaatcggt aaaaattttg aaattttctt catacttgga cttcctttcc gtttttattt
     2641 attgtacaat aaataaaaac ggatttaatt tttgttggga aaatgggaaa ttatgaaatc
     2701 aaaacttggc atcacactta gaaaagttcg taaaggaaaa caaattagtc tatgctctgt
     2761 agcagatgaa catctttcaa aatcacaaat atctcgtttt gaacgtgggg agtcagagat
     2821 atcttgtatt cgactaatca atattttaga caaattacat attactttgt aggggagttt
     2881 ttggctatcg ttgctcaatt gtctgattaa ggtcctaccc ttgatgaaac aattattatc
     2941 catgttttct ttattataga caaagtaaga agacgtttct cgaatgtaga ctttatattt
     3001 tttatgattt tcttcttcca taatatccaa ttgatagttg ggaatgaaaa taagaccgct
     3061 ctgtttgaca ccgaaagaca ccttgatata gacgccctta tcaactagct tctctatttg
     3121 gttctctgca agttccactt caaattcacg aacggtatct catttttcct taaatgtctt
     3181 aaaggcttcc tcaatctctt cagtggatac tttatcctta tctcgttctt cttggaaagc
     3241 atggtactgt tcctgtaaat tctctaatcc ttctgaagca acgacttcct tatttttaaa
     3301 ataatcttga aaaaatttga catcatataa tttcttatca cttatttttt gatgacccaa
     3361 acttatcttt tgattatttt cttccaggat aaaagttaca ttttttgttt taagtcaatg
     3421 gttagattca attcttttgc ttttgttatt aaatcttcta aagaattgac acggtttaac
     3481 aaaaattcta aacgactttc aatctcttgc ttagcaaaat gcgttctaaa aaattcttca
     3541 tcatatagat ctcgtttgct gagttggcgc cctcgaattg gttttatcat cgttctatct
     3601 gtcatcaaaa aacggctatg cttttgacta aaatcaatct gaacatgcaa ctgctttgct
     3661 ttctctaaaa aatcatcaaa cgatttagat tgctgaagca aaaaataaag acgttgtttc
     3721 aattcaaatt tatgactaga ttccttatat tttttataat ctcgaaaaga tcaggatttt
     3781 tagctaaaac tgccttacga aaatccttcc agaactgatg gtcaatctca ttagccacat
     3841 ccaaacgcca agcatcgata ttaaactctt caatccaata agtcgcaacc tttaaaagat
     3901 aattcttgac ctctggattg gctgtattta gcttaggcat atagtcctcg aaaccaaaaa
     3961 catgataggg taagtctctc ttattaacta gcttttcagt tgtcaatggg aattgttgaa
     4021 tatggaacca atccttataa gcagactgtt caccattttt gacgacattt tttcattgaa
     4081 gagattgcga accaatatga ttaaataccg catccagcat gactttcatg ccacgatgat
     4141 gcgcttgatc caccagttcc cgaaaggtct ccttgtctcc aaaataacgg tcaatttcaa
     4201 agtaatctgt cgtattgtac ttgtgattac ttgtagattc aaagatggga caaagatata
     4261 gtccagtaat acccaagtct tgcaagtaat tcatatgatc aataatcccc tgtaaatcac
     4321 caccaaagaa atcatcgctc ttaggtgtga cagatgaatc ccagtctaaa gtcccttctg
     4381 ggtttaatag agcattgcca ttggcaaatc tttcaggaaa tatctgatac cataccgtat
     4441 ttgaaaccca gtcaggaacc ttgcaggcat caatctcatg aagataaggc aacttaaatc
     4501 cattcccaat tgcatgaaga ttttctagag aattttccac acacccttta tcgccataca
     4561 aaatattttg accttctgta tctctgagct caaagagata ctggatacgt gcaaagtcaa
     4621 ctgacacttc aacctgccaa tggtcaaata aggtaccaga agttatcttg accatttctt
     4681 ttgtatcctg ataaaactct tccataaaga taaaagggtc cccatagtgc aagttgatgc
     4741 tttcaatgtc ccctttctta gttcgaattc gaatatggag tttcttatcc ttataaagat
     4801 aggcatactc cgactcaggc ctatggtaaa tagcactcaa ttccatcaat ttgtctccta
     4861 aagtattcat tcaaatttta cgcaaacgtt ttcaaactca ataaatccag tatactattt
     4921 ttgtaaacta tttgcaagga atgtccgttg ttaaaggaac tatcttttaa agtatacaaa
     4981 aagccttaaa aaaggctttc tctatatact aatcctcttt cggttttgaa aatacttcta
     5041 ctcgctgagc taggactttg atttctacag gtaggttatc tgatttatct ccgtcaacat
     5101 ctgactccaa ttcactatct gaagagattt taatattacg cgctttgata tactcgatat
     5161 tatcatttgc gacaacatcc ccttttaata aatcaggaat gacggataat ttggagaata
     5221 tagaggcatc tttcagaatc aaaatgttgg catagccgtc cttgttttct tcaaagattt
     5281 tcttatcagc gaagtaattt gtcaaaagga ccaaaacatg gctagcttca ccaacataat
     5341 ttccattttc tgtctcaacc ttaatgttaa agacctgatc tgtcatgaca gacttcatgg
     5401 tatttacagc ataggttaaa ataccgaatt ttgttttgtc ctctatttca acattgtgaa
     5461 tcgcctcagg cagagaaccg atactaaaga tataaccaaa atagttgtca tttgctttac
     5521 cgatatcaat cttattggtt aaatcaaaat ccagttcgtc aattgcgcca tcgatgtctt
     5581 gattgatttc caaaagtttt gtaatgaggt tacccgtacc gcctgggata atccctaact
     5641 taggaatgta gtctctctca tcaatacctg aaatgacttc attgacagtt ccatctccac
     5701 caaacacaac cactgcatca tactgctcac gagaagcttc ttcagcaaaa tgtgttgcat
     5761 ccagcgcttt ttcggtaatt ttggtttcaa catattcaaa gtattctttt gctttattct
     5821 ccagcttttc tttgtaatcc aaagccttct cgccaccaga agtagggttg ataattacca
     5881 ttgctttttt cattgatttt atccttaatt ttaaacagaa atgtttacat ttcgtcgtat
     5941 gcaagtaaat gtaatcctat tatacaatga aaatacagaa aagagaaatc tgacgtactg
     6001 gagattaata cgcttttatt ctattttccc atcgcctaac tacatccttt aagggttcat
     6061 ccaagtaaga ataggcctta tccttgatcc aatcaggaat accgtaagct gcctctgcta
     6121 tgttacaagt gattgctgcg agagtatcac tgtcgccacc aagtgagatg gcatttctta
     6181 tcgcatcttc gaagtctcta ctttcaagaa aggcgataat ggcttgaggg acagtttcct
     6241 gacatgtttc gttaaaacga tagttaggac ggatttcatc taaagtttga gatagattgt
     6301 aatcgtattc tttttctata taatccttaa ttcgtctttt acactctgta agattgtcgt
     6361 taattgattg ctcgtactct ctacaataac caccaaagta aaaacgacat agaaagatag
     6421 catcagctgt agccatagcg cctttgacac cttctggatg attatgagtt acctctgcag
     6481 aaagactcgt aagtcctcta gatgatggcc atataccagt tttcgcataa aaaccacagt
     6541 ccatgatcca agcacatgga gaaatacgca tagctgatcc attcccaaag ctattataag
     6601 gctcacggtt atcgctgttt agccatgcat taaaccgagc accgtaatca gcattcggat
     6661 acattctgcc atatttcttc atcgcgtcaa tgaaatcgtc tttttgtcca ccattcataa
     6721 ttgcttctgc aacagcacag gtcataaccg tgtcatctgt aaaaaagcag tccttccgaa
     6781 ataaaggaaa gtcctttgtt ttgatattgt tccattcgta aacagaaccg acaatatctc
     6841 caataattgc tccaagcatc agattcctcc ttgttcattt tgatgctttt tatattggtt
     6901 atataccata tttattttag aaaacaacat cctgttggat tttaaaaatt tcattttttt
     6961 caaaataggg ttttaccatt tctttccacc tagctctatg aaaattgatt gattttaaag
     7021 gagatagggc ataatttccc aatgcataac catcatttac ttcaacaaca agtgttctgc
     7081 catcgcgagt aacaccgata tctagtccat aagctattgg cgcatctttc caacatgata
     7141 tcgcttcatc aattacactt gcatcaaatt gtgcatgata atcacctgta tagggtcgaa
     7201 catctaatac gcgaccatct aacacaaaac aacgccattc agctatgaat tctacaacct
     7261 cactaatcca tataggatag tcgaaaggta gaccaatacc tattaaatca tgggttccat
     7321 taacaactct tccagtaaag acttttgaac cagctttagg cttaataaat tttccccaat
     7381 tatcaggtat attcacaatc tctcctaaaa taccagcata aatctttcga ccataaaact
     7441 ctttaagctc aataggatag tcataaaccg gaacgtttaa gcccatcatt tttagtaatg
     7501 ctctagtctc cattatataa tctacaacta tatcttcact tgttaactct tttatttcag
     7561 aaaaagattg atataaaata acttcttctc cttgtaagta ggcacctact tgagcattgt
     7621 atttattaat tgaaacctca cttggtaatt tactttgtct aatataaaca accatttcat
     7681 cactcctata tcactagtgt tacactgatt tgtaaaaaat aatagcaatt ttgctcttat
     7741 ttttttgagt aaatagctcc cataatatca tcgaaataat caacggtatt taggagtaat
     7801 tcaataacct gggactttgt tagtcgcatt ccccttctat ctctagcatc ttctactaaa
     7861 ttttcaagtt tctctagatt tttatcatcc aagctaatca ttattctatt tttatcggtt
     7921 gccattttca tcacctcaag ttaattctat cacaggtgta acacttgtgt caactggctt
     7981 ttataataca ttagtttaaa agtggagagg atttttaaca cagtaacttt aaatctttgg
     8041 tattaaaaaa ttttcacaat atttatagaa ataaaatctg tctcaaatca gttatcaaat
     8101 ctagtataaa ttatgagcgg ctactctaat actttccctc taaacaagaa aaagacttac
     8161 actcaaggat tttcttcccc cccttcgtta taacgttttg actcttttac tagcaaaggt
     8221 atatactcac aaggaacttt ggttgactat tgaatctctc caacttcttc tttaacatat
     8281 ccttctacat cttcaatctc tacaaacatt gggtctaagt gacacaagaa atgccaaact
     8341 tcgatccctt tttttctgta aagaatcgct tcaccgtctt cacttccgaa aaagcttctg
     8401 tcgatttcat atccgcggct ttctaagaag tcttttgctt tacgatagtt cgtttctctt
     8461 gtttcgacat aggttttaac ttcatggttg ttaacgacat atgcatcaat ttttgaatat
     8521 ccttcgatca ctctatcatt tttgagggat aaatttgaaa tctctttcca aataatgttt
     8581 acattttcct caggatcgaa cataaattta gataaaggaa caatatttcc gttaaaaata
     8641 atttccatat aatccggtat gtttttagga ttaaaatact ccacttcaaa accatcttct
     8701 gtttccagag tgtatcccgg gatttgagct acaaaggctt tcccatcttc tatggaatca
     8761 aatgctacta aatctttaga ataatcattt tggtacaatt ccaatataac catcgataat
     8821 ctctccattt tcattatcag gctaatgtaa ataagcacgt cacctgacca attcaggctc
     8881 tctgtatcat ctcatcatat ttcctactta ctttacgagt cctataccca gaacacacct
     8941 tatcgacctt cggtctcacc tcgttgcatt ggctgaacat ctacttttac ttcgctgatg
     9001 cttcaactcg tacaagcagt gataccgcct cagcgtgatg cgtcagtggg actcaaaagg
     9061 ttcggggaac cttttgagga ttaactacgt ttctctaata aacttacaca ttcaacttgt
     9121 tcatcattgt ccaaacctat gttgagattt tcttctataa ttggtagctt aaaagtaatg
     9181 gattttagcc attgtccgtt agattgtttt tcttcataaa cttgaatttc agaaatcaaa
     9241 gctgaaatta actgcctacg ctctacatca ttcatgactt tatagagctt atcaaaatag
     9301 atcagaacct tatatatgtt atctcctgta agcttttcag cttcaatagt ctgtttcttt
     9361 gctttcgcat caattagtga tgattctaat tcatctactt tgtcatacat acgatatagt
     9421 ctatcatcta aatcctgttt ccttctctta taatgcttat cttcaacatc taaattatct
     9481 atttcctcaa ttagcttaaa ctttgtagaa tgactctttc tcaattcctt ttggtaatta
     9541 tctatttctt tttctatttc agaggtatcc accttcatgt tgattttttc ttgcatcata
     9601 gaagcaaatt tcggattact tactatcttg acaatcacct ctgcaacagc atcatctaac
     9661 aattcttctc taatttgctt actgaatgta cacttatgac ctcttatcat ctgcctatgg
     9721 ttacaaccat agtaataaaa atctttatac tttgtgccat ctttcttttt cttgatacac
     9781 ttgttcccaa acattcccac tccacatatc gggcatttta caattccaga aagcaagtgt
     9841 gtgcgtgtat cttttccttt attcacatgc tcatatttct ttgcttgaga ttttagctta
     9901 acctgagcag cttgccaaac ttcatcggaa actatagctt catgtatccc ttcagatatt
     9961 agatattcat cttgttcaac ctgcttatat tcatttcttg taccatgaac tttttctaaa
    10021 gttcttcttc caaatgctat tttcccatta tatacaggat tctttaatat ctttcttata
    10081 agacctgcat caaacaaagg attcttacca ttctgtcttg ggatttttct aattccatga
    10141 ttctctaagt atttagatat cccattggct cctatcgtag tatttacata ctggtcgaaa
    10201 atcgttctta ttgcaactgc ctcttcctca tttataaaca gcttgccgtc ttcaagttta
    10261 tatccatacg gagcaaagcc accattccat tttccttccc ttgctttttg aatgcgacct
    10321 tccattgttt gaatacggat gttttctctt tctatttcag ccacagctga taaaacagaa
    10381 atcattagtt tcccagcatc tttagatgaa tcaatgccat cttcaacgca gataagatta
    10441 actccataat cctgcattat ctgaagtgta gaaagaacat cagcggcatt tcttgcaaat
    10501 cttgataact taaacacaag aacaaaagat actccatctt ttccagattt tatatcttcc
    10561 atcattcgat tgaactgtat tctaccttca atagacttgc cagacttccc ggcatcttca
    10621 tactctccaa caatttcata atcgttgtaa atagcaaaag ctttcattcg tgatttttgt
    10681 gcctctaacg aatacccctc tatctgtatt gacgtagata ctcgtgtata gaggtatact
    10741 tttatttttt cttttgacat agtattaatc tcaatataat ttttctatat catatataat
    10801 ttttttaatt taagtttgga ctatcatttc aagtatatta taacactttt attagtccgt
    10861 ctcaatttgt gtttttgcca tgtcaaaact atttttcatc tcttgatttt ttgctggcgt
    10921 tggatcgggt agattatcta aatctaaagc accagcatat tttgcaatca gatttgctat
    10981 taaatcagcc aatccattcc agtcattgtc caatatatac ctcctctaaa gttttatatc
    11041 taataattat ttgtttaatt aagttttttg acattgacaa gtgctttgga ttagcaacat
    11101 aggaatctca cctccgcctc tattccggat gagccggctt caaccttaga agtatcatta
    11161 tcctcatttt cttcatagcg gatagggtat ccctccctat attcaaactc ttacttatcg
    11221 ctcactttct ttttacttag cagaactttt tttgccgaat tattcagccg aaagatcttg
    11281 acggataggt tattacgctc caaaaataat taacgtcttg tcttggtcta ttcaattgtt
    11341 aaggttcaaa atttatcgag agttattaat ctttttaaaa tttgaccatc agaaaatatt
    11401 tatcttaatg taacaaaatt ctataaatta ccctcttata cttaacagtg aaaagaagtc
    11461 tttcttggta accaattttg aaatagaatt tgcttatata aaaaggtcaa attcccactg
    11521 cataagtagc agtgaaaatt agaccctctt ggtaactgtc atctaaaagt cttctagcat
    11581 ttctttaatt ttctaatcgc ctttaaagtg tcatatttat atttttttat ctctggcata
    11641 ctttgatacc tttttagact taaagtcttt aatagtgcct ttccacctct ttttatctat
    11701 aaagattctc ctacatcata attcattttt ttatttaaac ctttctgtct tagtttgtct
    11761 ttatcttctt cataccattt taagattgtc acatagtggt tttgataggt cttaccactg
    11821 ctttccatgt atctggatag tttatttatc attatatctg tgtgtgagtt taatttttct
    11881 tttagatttt tatattcttc tttgcttaac cttacatttt tgaattctcc ataaaaaatg
    11941 ggggtggact ttttatctat ctctccctct ctctctttat ctatctctat atctttccat
    12001 gtaattccaa tctggagtac ctctactgtc tatcggtaat ttaattttga tatctggcaa
    12061 tactgtgcta gatatttgat ctttatattc agtatttttt aaagcttgcc taataattga
    12121 agttaaatag aatgctactt ctttattcaa ttctttattt tttaatttta aacaatgaat
    12181 tttcatatct aggcttgctt tatatttatg ataaaagact gctcctaaaa atgaaacaga
    12241 tataaaattt tcaaaaactc tataattttt atcatctata tcttcgtagt aacctaagat
    12301 accattgtca atatttgtag cactaattct aggagttttt ccatcgagta aatatctttt
    12361 tggaatagat gagcctgttg gtacttgact cgatttcccc tttttttcgg taataaatat
    12421 ttctttttat tttgttgtct gatatttttc ctacctgtcc tttgtaggat gagtattttc
    12481 tagattttct tgaataactt tttacttgaa gttttagctt ttgaactagt cgttgtactt
    12541 tctttttgtt tattatcagt cctgatcttt ttaatattgc tgttattctc tatatcctat
    12601 ttttcattca tgatattctt ttactaattt tatcttaaat tctgtgctgt atttgccatt
    12661 aaaaaactga cctcctttag ttagtttttt ggcctaactt ttgagggtca gttcaaaatt
    12721 tgcgactttt aaatgaattc caatattcaa ttattaagag ctaacatggt gcttgccaat
    12781 aggaatcatt agaggcgaat tggaaatagg gtcacgtata atttttgctt caagattaaa
    12841 gatatcttta actagtttat catttagtat atcttcaggc tttccctctg caacaagttt
    12901 accttcttta attgcaaata ggtaatcagc gtatcttgct gttagattta tatcgtgcaa
    12961 aatcatgcaa atggttgtct tatatttttg gtttagatca gtcaagaggt ctaatagttc
    13021 tatttgatat gagatatcca agtaagtagt tggctcatct aaaagtagga tacttgtatc
    13081 ttgggctagg gctagagcta tccatactct ttgcctttga cccccagaaa gttcttcaac
    13141 taggttattt gctagatctt caacattggc cttaaccatt gatctgttta ttatttcaag
    13201 gtcatctttt ccaagactct taaaaggctt tctgtagggg aaacgaccac ggcttacaag
    13261 atcagctact gttattgatt cagggattat tggagattga ggtaatatag ctatgtgttt
    13321 tgctaaatct ttttctttat aagaattaat tgatttatta tcaagcaata cttctccctc
    13381 taatggcttt ataagtcgag acaaggtttt aatgagtgtt gatttcccac aaccatttga
    13441 cccaataata actgatattt tttcttcagg tatttttata tttatatttt ccaagattat
    13501 tttttcatca taaccgcagg taagattatt tgaccacaga cctttcatta tatattcctc
    13561 ctgttcattt ttattagtaa gtatattaag tatggtgaac ctaacaagcc agttacaaca
    13621 cctactggat atctagctgg taaaatattt tgagagaata tgtctgataa caaaactagt
    13681 aaaattccaa ccaatccagc taatattggg cttcttttct tgccaatatt taaggctatg
    13741 ggaccagcta aaaaagatat acaagctatt ggtcctgtaa ttgaagtaga aaaagcagtt
    13801 aaagatacag cgcaaaaaat taaaacaagc cttgaaagct cgggatttgc tccaagtccg
    13861 attgctattt cttcaccaag ttcaataatt tctagtcttt tattaaaaaa taaaactaat
    13921 atagtagcaa taatacttac tattagaaca agaggtatgt catctaactt tgtaaaagat
    13981 aaagagccac tgagccatct cataacttct tgtaattcat atcttgctac tttcaacaat
    14041 aaaaatgagg tgcctgctct tgtgacagct tgaaaaccaa tacctaatat tatcagtctt
    14101 gctgctgaaa aaccatcttt tttagctagt aaaaataata ttaaagatga tgttagtcca
    14161 caagttattg aaataattcc agtagttaaa ctatttgttt ttaataccaa tatgcaaaag
    14221 accgctgcaa tagatgaaga acttgtgaca ccgattatat caggacttgc aagaggattt
    14281 cttaacatag tttgaaagat aaatcctgcc aatccaaaag accagccagc tataattcct
    14341 gctaataatt ttggtaatct aatttccata atcgaaaaac tagctccagg aacagtttca
    14401 ctatttaaga ctttaatcaa agttgaaaaa gaataacttt catctccgat aagtaaaatg
    14461 aaaaatgata gactgattat tattaataaa aatagtgagg aaaatagtgt tattctattt
    14521 tttctttttt gaatacctat aattaaattt tgcattagtt attaacccct ctatttttca
    14581 tagttacata aataagtact ggacccccga ttattgcagt aattatccct acttcaattt
    14641 cacctggttt acctaacata cggccgatta tatcacatat aagcaagagc tctgcaccta
    14701 taaaagatga agaaatggtc attgtgcgta tatctttgct tataaataag ccacaaaagt
    14761 gaggaactat aagacctacg aagccaatag gtccaccaat tgcagtaata cttgaacata
    14821 aaagcacact tgcaattatt gcaagtgatc ttatcctatt aacattaact ccaagaccaa
    14881 cagccatttc atcacccata gctaaagcgt ttaaatctga tgaaataaat atagctatca
    14941 agtgacctaa aattataaaa ggtagtagtg tagatataga agataatgta gctgctccaa
    15001 ggctacctat ttgccaaaat ctaaatttgt ctaagacgtt attattcggt aaaattaaaa
    15061 aacttacaaa actgcttaaa gccatactaa cacaagttcc tgataaggct agttttatag
    15121 gggtaaggcc tgcttttccg cttacagcaa tcgcgtatac aaaaattgca cttactaagc
    15181 caccaatgat tgcaaaactt atatggctta tgcttgatga aattcctaaa aaagaaagac
    15241 caattactac actaagactt gctcctgtgt ttataccgag tatacctgga tcagctattg
    15301 ggtttctagt aactgattgc atcaatacac cgcttatggc aagactagaa cctgctaaaa
    15361 ttgcaaaaat agttctaggt attcttttat atataattga tttaataaaa tcatcatttg
    15421 tattgcccat tccaaaagtt gctaaaaaat ctctgatatt aatttctttt gttccaagct
    15481 ttaatgacaa aaatattgta ataagaagac ctaggcagat tacaaaaatt atgcctaggt
    15541 ttatttgttt tttatttcgc atttttgcat gcatttccta aaagatttag gtattcttca
    15601 atagtatagt ttattgaaag tggtgttgga gtgcatgagg ctgctaacgg tgtattatct
    15661 gaaattacag caacggcacc atttttaatt gcatttattt tacctaaaag aggatctttt
    15721 tgtaaagctt caagagtttt atcatcacca taagttatga ttacatcagc atcatttatc
    15781 ttatttgctt cttctgcaga aatttccttt gcaaaactat cttcactctc aaattctttt
    15841 aatgattcag ggaaaactag acctaaatct gttaaataat ttgctcttgg atctttgcta
    15901 gtataaatcc agaattttga tgtatctgca gcattaatca tagtaaataa tacttttttt
    15961 cctttgattt ttcctttgat ttctggatgt ttttctaatt ctttggatat acgagcttca
    16021 gtatttttga ttaactcatc accttctttt tccataccta aggcttttga atcaatttta
    16081 atcatatctc tccataaagt ttgccaaggt ttagatttgt atgctgctac aggagcaatt
    16141 tttgatagag tgtcataatc ttctttagtt ataccagaat aacctgctaa gataacatct
    16201 ggtttagaat ttgatattgc ttcaaagtta agtccatcca aatcgtcaaa taggttagct
    16261 ttaccattta gttctttgat tttttcttct gtccatggta aaactccttt atcagcactt
    16321 acaccgtaat ttgcttttga aaatccaaca ggaactattc ctaaagctaa tgctacatca
    16381 tgatttcccc aagcaatagt tgcaactctt tcaggttttt tatctaatat agtttgaccg
    16441 aaagcatgat ctaaaactat tttatcaggc gcatgctctt tagaagtatt ttcttcattt
    16501 ttaacagaat tagaagaaca agcgctaaaa actatacaca tagctaaaat agctattaga
    16561 aaaaatttgt ttttcataaa aactccttaa acatatttca agtctattgt atcgataata
    16621 attatcattg tcaatagata cagttacaaa tttaataata taattttttc aaaaaagg
//
LOCUS       DAVBCB010000050        16132 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVBCB010000050 DAVBCB010000000
VERSION     DAVBCB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.050"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            145..6831
                     /locus_tag="VQU28_001632"
     CDS             145..6831
                     /locus_tag="VQU28_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HET2476959.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTVEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQTSNQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDVDVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGFKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVARVRTLIGKEPKMPKTVGFVYSDGSREKLPVTWSQ
                     VDVSQAGVVTVKGTANGREVEAHVEVLAIAKELPTVKRIAPNTDLNSVDKSVSYVLTD
                     GSVQEYEVDSWEITEVDKAKLSVAGSRIQMTGQLAGETIHATLVVEEGNAAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLKAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQKPTLPTTVTVEYDKGFPKAHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRIEQYA
                     KEGVFTVNGRLEDTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRTNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQIRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLTVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLRFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IQLTKEKPVSAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGKERIFT
                     AINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSEASQTN
                     KAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(7079..7935)
                     /locus_tag="VQU28_001633"
                     /pseudo
     CDS             complement(7079..7935)
                     /locus_tag="VQU28_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8146..8505)
                     /locus_tag="VQU28_001634"
     CDS             complement(8146..8505)
                     /locus_tag="VQU28_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HET2476960.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSILAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            8597..9553
                     /locus_tag="VQU28_001635"
                     /pseudo
     CDS             8597..9553
                     /locus_tag="VQU28_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            9537..10094
                     /locus_tag="VQU28_001636"
     CDS             9537..10094
                     /locus_tag="VQU28_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HET2476961.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            10087..10341
                     /locus_tag="VQU28_001637"
     CDS             10087..10341
                     /locus_tag="VQU28_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476962.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            10353..12407
                     /locus_tag="VQU28_001638"
     CDS             10353..12407
                     /locus_tag="VQU28_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HET2476963.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            12586..13446
                     /locus_tag="VQU28_001639"
     CDS             12586..13446
                     /locus_tag="VQU28_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HET2476964.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQU28_001640"
     CDS             13657..14367
                     /gene="ccdA2"
                     /locus_tag="VQU28_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2476965.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            14387..14953
                     /locus_tag="VQU28_001641"
     CDS             14387..14953
                     /locus_tag="VQU28_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HET2476966.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            14964..16076
                     /gene="msrB"
                     /locus_tag="VQU28_001642"
     CDS             14964..16076
                     /gene="msrB"
                     /locus_tag="VQU28_001642"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HET2476967.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
BASE COUNT         4901 a         3052 c         3565 g         4614 t
ORIGIN      
        1 catatctacg gcaaggcgac actgacgaag tttgaattgg attttcgcag agtgtaaatt
       61 gttgaaacta ggttgtcata ccatgtatac cacttgggct ttggtagtag ctaactgcac
      121 taaatataat ataaggaggg ttttatgggg aaaggccatt ggaatcggaa aagagtttat
      181 agcattcgta agtttgctgt gggagcttgc tcagtaatga ttgggacttg tgcagttcta
      241 ttaggaggaa atatagctgg agaatctgta gtttatgcgg atgaaacact tattactcat
      301 actgttgaga aacctaaaga ggaaaaaatg atagtagaag aaaaggctga taaagctttg
      361 gaaactaaaa atgtagttga aaggacagaa caaagtgaac ctagttcaac tgaggctatt
      421 gcatctgaga agaaagaaga tgaagccgta actccaaaag aggaaaaagt gtctgctaaa
      481 ccggaagaaa aagctccaag gatagaatca caaacttcaa atcaagaaaa accgctcaag
      541 gaagatgcta aagctgtaac aaatgaagaa gtgaatcaaa tgattgaaga caggaaagtg
      601 gattttaatc aaaattggta ttttaaactc aatgcaaatt ctaaggaagc cattaaacct
      661 gatgtagacg tatctacgtg gaaaaaatta gatttaccgt atgactggag tatctttaac
      721 gatttcgatc atgaatctcc tgcacaaaat gaaggtggac agctcaacgg tggggaagct
      781 tggtatcgca agactttcaa actagatgaa aaagacctca agaaaaatgt tcgccttact
      841 tttgatggcg tctacatgga ttctcaagtt tatgtcaatg gtcagttagt ggggcattat
      901 ccaaatggtt ataaccagtt ctcatatgat atcaccaaat accttcacaa agatggtcgt
      961 gagaatgtga ttgctgtcca tgcagtcaac aaacagccaa gtagccgttg gtattcagga
     1021 agtggtatct atcgtgatgt gactttacaa gtgacagata aggtgcatgt tgagaaaaat
     1081 gggacaacta ttttaacacc aaaacttgaa gaacaacaac atggcaaggt tgaaactcat
     1141 gtgaccagca aaatcgtcaa tacggacgac aaagaccatg aacttgtagc cgaatatcaa
     1201 atcgttgaac gaggtggtca tgctgtaaca ggcttagttc gtacagcgag tcgtacctta
     1261 aaagcacatg aatcaacaag cctagatgcg attttagaag ttgaaagacc aaaactctgg
     1321 accgttttaa atgacaaacc tgccttgtac gaattgatta cgcgtgttta ccgtgacggt
     1381 caattggttg atgctaagaa ggatttgttt ggttaccgtt actatcactg gactccaaat
     1441 gaaggtttct ctttgaatgg tgaacgtatt aaattccatg gagtatcctt gcaccacgac
     1501 catggggcgc ttggagcaga agaaaactat aaagcagaat atcgccgtct caaacaaatg
     1561 aaggagatgg gagttaactc catccgtaca acccacaacc ctgctagtga gcaaaccttg
     1621 caaatcgcag cagaactagg tttactcgtt caggaagagg cctttgatac ttggtatggt
     1681 ggcaagaaac cttatgacta tggacgtttc tttgaaaaag atgccactca cccagaagct
     1741 cgaaaaggtg aaaaatggtc tgattttgac ctacgtacca tggtcgaaag aggcaaaaac
     1801 aaccctgcta tcttcatgtg gtcaattggt aatgaaatag gtgaagctaa tggtgatgcc
     1861 cactctttag caactgttaa acgtttggtc aaggttatca aggatgttga taagactcgc
     1921 tatgttacca tgggagcaga taaattccgt ttcggtaatg gtagcggagg gcatgagaaa
     1981 attgctgatg aactcgatgc tgttggattt aactattctg aagataatta caaagccctt
     2041 agagctaagc atccaaaatg gttgatttac ggttcagaaa catcttcagc tacccgtaca
     2101 cgtggaagtt actatcgccc tgaacgtgaa ttgaaacata gcaatggacc tgagcgtaat
     2161 tatgaacagt ctgactatgg aaatgatcgt gtgggttggg ggaaaacagc aaccgcttca
     2221 tggacttttg accgtgacaa cgctggctat gctggacagt ttatctggac aggtacggac
     2281 tatattggtg aacctacacc atggcacaac caaaatcaaa ctcctgttaa gagctcttac
     2341 tttggtatcg tagatacagc cggcattcca aaacatgact tctatctcta ccaaagccaa
     2401 tgggtttctg ttaagaagaa accgatggta caccttcttc ctcactggaa ctgggaaaac
     2461 aaagaattag catccaaagt agctgactca gaaggtaaga ttccagttcg tgcttattcg
     2521 aatgcttcta gtgtagaatt attcttgaat ggaaaatctc ttggttttaa gactttcaat
     2581 aaaaaacaaa ccagcgatgg gcggacttac caagaaggtg caaatgctaa tgaactttat
     2641 cttgaatgga aagttgccta tcaaccgggt accttggaag caattgctcg tgatgaatct
     2701 ggcaaggaaa ttgctcgaga taagattacg actgctggta agccagcggc agttcgtctt
     2761 attaaggaag accatgcgat tgcagcagat ggaaaagact tgacttacat ctactatgaa
     2821 attgttgaca gccaggggaa tgtggttcca actgctaata atctggttcg cttccaattg
     2881 catggccaag gtcaactggt cggtgtagat aacggagaac aagccagccg tgaacgctat
     2941 aaggcgcaag cagatggttc ttggattcgt aaagcattta atggtaaagg tgttgccatt
     3001 gtcaaatcaa ctgaacaagc agggaaattc accctgactg cccactctga tctcttgaaa
     3061 tcgaaccaag tcactgtctt tactggtaaa aaagaaggac aagagaagac tgttttgggg
     3121 acagaagtag caagagttcg tacattaatt ggtaaagaac caaaaatgcc gaaaacagta
     3181 ggttttgtat acagcgatgg tagtcgtgaa aaacttccag taacttggtc tcaagtcgat
     3241 gttagtcaag caggagttgt gactgttaaa ggtacagcaa atggacgtga ggtagaggct
     3301 catgtcgagg ttctagcaat tgcgaaagaa ttaccaacag ttaaacgtat tgctccaaat
     3361 actgacttga attctgtaga caaatctgtt tcttatgttt tgactgatgg aagtgtacaa
     3421 gagtacgaag tagatagctg ggagattacg gaagtagata aagccaaact ttcagtagct
     3481 ggatcacgta ttcaaatgac tggtcagtta gctggagaaa ctattcatgc aacccttgtg
     3541 gtagaagaag gaaatgctgc agcacctgta gtgccaactg ttactgttgg aggtgaggca
     3601 gtaacaggtc ttactagtcg acaaccaatg caatatcgta ctctatctta tggtgcccaa
     3661 ctgccagaag tcacagcaag tgctgaaaat gctgatgtga cagttcttca agcaagcgca
     3721 gcaaacggca tgcgtgcgag catctttatt cagcctaaag atggtggtcc tcttcaaacc
     3781 tatgcaattc aattcctaga agaagcacca aaaattgctc acttgagttt gcaagtggaa
     3841 aaagctgaca gtctcaaaga agaccaaact gtcaaattgt cggttcgagc tcactatcaa
     3901 gatggaacgc aagctgtatt accagctgac aaagtaacct tctctacaag tggtgaaggg
     3961 gaagtcgcaa ttcgtaaagg aatgcttgag ttgcataagc caggagcagt cactctcaaa
     4021 gcggaatatg agggagctaa aggccaagtt gaactcacta tccaagccaa tactgagaag
     4081 aagattgcgc aatctatccg tcctgtaaat gtagtcacag atttgcatca aaaacctact
     4141 cttccaacaa cagtaacggt tgagtatgac aaaggtttcc ctaaagctca caaagtcact
     4201 tggcaagcta ttccgaaaga aaaactagac tcctatcaaa catttgaagt actaggtaaa
     4261 gttgaaggaa ttgaccttga agcgcgtgca aaagtctctg tagaaggtat cgtttcagtt
     4321 gaagaagtca gtgtgacaac tccaatcgca gaagcaccac aattaccaga aagcgttcgg
     4381 acatatgatt caaatggtca cgtttcatca gctaaggttg catgggatgc gattcgtata
     4441 gagcaatacg ctaaggaagg tgtctttaca gttaatggtc gcttagaaga tactcaatta
     4501 acaactaaac ttcatgttcg cgtatctgct caaactgagc aaggtgcaaa catttctgac
     4561 caatggaccg gttcagaatt gccacttgcc tttgcttcag attcaaatcc aagcgaccca
     4621 gtttcaaatg ttaatgacaa gctcatttcc tacaataacc aaccagccaa tcgttggaca
     4681 aactggaatc gtactaatcc agaagcttca gtcggtgttc tgtttggaga ttcaggtatc
     4741 ttgagcaaac gctccgttga taatctaagt gtcggattcc acgaagacca tggagttggt
     4801 gcaccgaagt cttatgtgat tgagtattat gttggtaaga ctgtcccaac agctcctaaa
     4861 aaccctagtt ttgttggtaa tgaggaccat gtctttaatg attctgccaa ctggaaacca
     4921 gttactaatc taaaagcccc tgctcaactc aaggctggag aaatgaacca ctttagcttt
     4981 gataaagttg aaacctatgc tgttcgtatt cgcatggtta aagcagataa caagcgtgga
     5041 acgtctatca cagaggtaca aatctttgcg aaacaagttg cggcagccaa acaaggacaa
     5101 ataagaatcc aagttgacgg caaagactta gcaaacttca accctgattt gacagactac
     5161 taccttgagt ctgtagatgg aaaagttccg gcagtcacag caagtgttag caacaatggt
     5221 ctcgctaccg tcgttccaag cgttcgtgaa ggtgagccag ttcgtgtcat cgcgaaagct
     5281 gaaaatggcg acatcttagg agaataccgt ctgcacttca ctaaggataa gaacttactt
     5341 tctcataaac cagttgctgc ggttaaacaa gctcgcttgc tacaagtagg tcaagcactt
     5401 gaattgccga ctaaggttcc agtttacttc acaggtaaag acggctacga aacaaaagac
     5461 ctgacagttg aatgggaaga agttccagca gaaaatctga caaaagcagg tcaatttacc
     5521 gttcgaggcc atgtccttgg tagtgatctt gttgctgagg tcactgtacg agtgacagac
     5581 aaactaggcg aagctctttc agataaccct aactatgatg aaaacagtaa ccaggccttt
     5641 gcttcagcaa ccaatgatat tgacaaaaac tctcatgacc gcgttgacta tctcaatgac
     5701 ggagatcatt cagaaaatcg tcgttggaca aactggtctc caacaccatc ttctaatcca
     5761 gaagtatcag cgggtgtgat cttccgtgaa aatggtaaga ttgtagaacg gactgttgcc
     5821 caagccaaac ttcgtttctt tgcagatagt ggtacggatg caccaactaa actcgtttta
     5881 gaacgctatg tcggtccaga gtttgaagtg ccaacctact attcaaacta ccaagcctac
     5941 gacgcagacc atccattcaa caatccagaa aattgggaag ctgtgcctta tcgtgcggat
     6001 aaagacatcg cagctggtga tgaaatcaac gtaacattta aagctgtcaa agccaaagct
     6061 atgagatggc gtatggagcg taaagctgac aagagcggtg ttgcgatgat tgagatgacc
     6121 ttccttgcac cgagtgaatt gcctcaagaa agtacgcaat cgaagattct tgtagatgga
     6181 aaagaacttg ctgatttcgc tgaaaatcgt caagactatc aaattaccta taaaggtcaa
     6241 cggccaaaag tctcagttga agaaaacaat caagtagctt caactgtggt agatagtgga
     6301 gaagatagcc ttccagtact tgttcgcctc gtttcagaaa gtggaaaaca agtcaaggaa
     6361 taccgtatcc agttgactaa ggaaaaacca gtttctgctg tacaagaaga tcttccaaaa
     6421 ctcgaatttg ttgaaaaaga tttggcctac aagacagttg agaaaaaaga ttcaacactg
     6481 tatctaggtg aaactcgtgt agaacaagaa ggaaaaactg gtaaagaacg tatctttaca
     6541 gcgattaatc ctgatggaag taaggaagaa aaactccgtg aagtggtaga agctccgaca
     6601 gaccgcatag tcttggttgg aaccaaacca gtagctcaag aagctaaaaa accacaagtg
     6661 tcagaaaaag cagatacaaa accaattgat tcaagtgaag ctagtcaaac taataaagcc
     6721 cagttaccaa atacaggtag tgcggcaagc caagcagcag tagcagcagg tttagctctt
     6781 ctaggtttga gtgcaggatt agtagttact aaaggtaaaa aagaagacta gaaattttga
     6841 taccttcttt atcattaaag accaaagaaa aactcttgtc ctaaatagaa gataaagagt
     6901 ctggaacaaa atagttctca accacaaaaa agctagatat ttccaattgc ggaactctag
     6961 ctttttaatt ttgagtcgtt ctcttattgt atagtagatt gaaactagaa tagtacactt
     7021 ctacttctaa aacattgtta gaaatcaatt tgactgtcct tatcgatttg tcctgttctt
     7081 atttcatttc actatgtttt aggaggttat tggccataag taccaatccc atgtcaattc
     7141 tcacttgacg cttccctctc agattacatc tcttgtaacc caaacaagcc tttatctgcc
     7201 caaagacagg ttccacatca atcttgcgtt gagcgaaaat ctgtctacct tggggagata
     7261 aaagcgcctg acattcttta gctttcaagt tttgatagcg ttcgttcata tacagtccct
     7321 tttgagggac tgattcaggt tcatcagcgt agtaaacctt gatttcctgt tgaaagtctg
     7381 tctgtgtttt ctggtacttg atatggtgaa aacgatagca ccagccatca ggatgtgtgt
     7441 agctatcctc cttgtcatta tagtgccaat tcgctaagtt tttagctgac tgtttatacc
     7501 ctctcttttg ttccttatca aacatggcat atttaatcag aaggggagtg atttcttctt
     7561 gaaaatagac ctgtatctgt tcttgaagtt tggcggagaa tttctctgtc gttttcttcc
     7621 acacaaaact atacttgttg gcattggctt caatcttagt tccgtcaata aacaaacaat
     7681 ctaaggtcac taactcttcc atttttcaat ttttcgacca gagaaaatcc cttgtgaata
     7741 ggcaaataga agagtagata caagcatttt agggtgataa gacgggcgat caaaggcatg
     7801 atagaaggcg tggaagtgac aatcctccaa ggtattcacc actttttcaa tagtaaaaac
     7861 gagatgatct tgtggcaaga aggaactgat ttctagtggt aaagttgttt gatttgtgtt
     7921 atagtgaata tgcatgggat agcctttcta aatggtttat taagcaattc tattttacca
     7981 agactatcgc aaccatgcaa aaaagcaacg gcaaatccgt tgcctttttt ggagataaga
     8041 gactattgtc ccagactctt ttctttgtat aaaattgtat agtgttattg tctatactat
     8101 tttgtagaga aagcaaaaat ccctagaaaa tgcttaggga ttcgactatt tcattaagaa
     8161 caattcaaca atcatttcta tatacatgat aagggttaat aagaatcctg cacgccagaa
     8221 gaatttgata aatttaggat agtaaaagct gcgttttttc aaaaggaaga aaaagacgag
     8281 aataatggct aggatcgaga gggctagccc cagtctaggg aggaaattat gggtaaaggt
     8341 tttagctgta atcaagtaat actcaaatac caagactgga aaagccaaat ccgcaaagtt
     8401 tcgtcctagt ttttttaatc taaaaagttt ggttaggata atgcagacga ctaaggtcag
     8461 tatcaataat aaaatagatg ctaatttcat taaaatcata cccatattgt atcataaaaa
     8521 aaggctaatg gaaacgagaa acagaggaat ttataggaaa gtcaggcttt tgagattgtg
     8581 ggtgattttt gttataatga aagttataaa atcttataat accttgaatg attattatcg
     8641 aaaactcttt ggagaaaaga cttttaaagt ccctattgat gcgggatttg actgtcccaa
     8701 tcgtgatgga actgtggctc atggaggctg tactttttgt acggtttccg gttctggaga
     8761 tgccattgtg gcaccagatg cgcctatccg tgagcaattt tataaggaaa ttgactttat
     8821 tcatcgtaag tggccagatg ttcagaagta tctggtttat tttcaaaatt ttaccaacac
     8881 ccatgaaaag gtggaagtta ttcgagagcg ttatgaacag gctatcaatg agccaggtgt
     8941 ggtaggaatc aatattggaa cgcgcccaga ctgtttacca gacgaaacca tcgaatattt
     9001 ggctgagtta tcggagtgca tgcatgtgac ggttgaattg ggcttgcaaa ctacttatga
     9061 agcaacctct gacctgatta accgtgcgca ttcttatgaa ttgtaggtgg aaacggtcaa
     9121 gcgtttgaga aagtatccaa aaattgagat tgtttcccat ttgatcaatg gtttgcctgg
     9181 tgaaacccat gagatgatgg ttgaaaatgt ccgccgttgt gtcacggata acgatattca
     9241 agggattaaa ctgcatttgc tccatcttat gacaaatact cgtatgcaac gagattacca
     9301 tgaggggcgt ttacaattga tgagtcagga cgaatatgtc agggtcatct gtgaccaact
     9361 ggaaattatt cccaaacata tcgtcatcca tcgaattaca ggagatgcgc ctagggatat
     9421 gctgcttggg cctatgtgga gcctcaataa atgggaagct ctcaacagca ttgagatgga
     9481 gatgcgacgt cgtggaagtg ttcaaggatg caaggctgta aaacaggagt ttgaaaatga
     9541 aaagaccact tgagatggca catgattttt tggctgaggt cgtgacaaaa gaggatgtcg
     9601 tagtggatgc gactatggga aatggtcatg acacgctttt tttagccaag ctagccaagc
     9661 aagtctatgc ctttgatatt cagaagcaag ccttggaaaa gacccaagag cgtttgcatc
     9721 aggctgactt gacaaatgcc cagttaatct tgcaaggcca tgagacactg gaccagtttg
     9781 tgataaaagc taaggcaggg atttttaatc tgggctattt gccgtcagct gataagtctg
     9841 tcatcacccg accgcagaca acgattgagg cattagaaaa gctatgtggc ttacttgtca
     9901 aaggtggacg aattgctatt atgatttact atggtcatga aggaggcgat ctcgagagag
     9961 atgctgtctt ggattttgtg atccagttga accaacaaga gtacacagct gccatttacc
    10021 gaactttaaa ccaagtcaac aacccgccgt ttttagtgat gattgaaaaa ttagagagat
    10081 acagacatgg ataaacaata cctacacgaa aagctggatg ccatgcgcca gaattttgtt
    10141 gaatcaaccc accacgaacg atcggtgggc gtgctagacc aagcgcatat gagcaaaaaa
    10201 atgcttaaaa tcaagaaaaa attagttgct cttgaaatgg aacggtgcca gagaaaaatt
    10261 gagcacaaag actgttccaa gattgaccaa aaaatcaaag agcagaagga gatatttgaa
    10321 tcctgttgta aaaaagatta aggaggacgt gcgtggaatt actgatttac ctcatcctat
    10381 ttttactggt tttgattgtc tcaagtacaa ccaataagct cctgcccttt ttgcctctcc
    10441 ctttggtgca gattcttttg ggaattgtga ttggtctctt tttacccaat actgactttc
    10501 accttaatac ggagttgttt ttggcactgg ttatcggacc cttgcttttc cgagaggctg
    10561 aagaagcaga tgttacggct attttaaaac actggcgaat cattgtttat ctcatatttc
    10621 cagtgatttt tatctcgacc ttgagtttgg gtggcttggc ccatcttctt tggttcagcc
    10681 ttcccttggc agcttgcttg gctgttgggg cagcccttgg tcctacggac ttggtggcct
    10741 ttgcctctct ttcggagcgt tttagctttc ctaagcgcgt gtccaatatt cttaagggcg
    10801 aaggactctt gaatgatgct tctggtttgg tggcttttca ggtagctttg acagcttgga
    10861 caactggagc tttttctctg gggcaagcta gcagttcgct catcttttca atcctaggcg
    10921 gttttttaat tggattttta acggccatga ccaaccgctt cctccatacc ttcttgctaa
    10981 gtgtgcgcgc aacggatatt gccagtgaac ttttattaga attgagtttg cctctagtga
    11041 ccttctttct ggcagaagaa gttcatgttt caggtattat tgccgtcgta gttgctggaa
    11101 ttttaaaggc aagtcgcttc aagaaaatca cgctcctcga agcccaagtg gatacggtga
    11161 ccgagacggt ctggcataca gtgaccttta tgctcaacgg ttctgtcttt gtgattttag
    11221 ggatggagtt ggaaatgata gcagaaccta tcttgaccaa tccaatctat aatcctctac
    11281 ttttattgct atctctcatc gcccttacct ttgtcctctt tgtcattcgt tttattatga
    11341 tctatggcta ttatgcctat agaacccgac gcctcaagaa aaagctaaat aagtatatga
    11401 aggacatgtt tctcttgacc ttttcaggtg ttaagggaac ggtgtcgatt gctacgattc
    11461 tcttgatacc aagtaatcta gaacaggagt atcctctttt gcttttcctt gttgcaggtg
    11521 tgacgcttgt cagcttttta acaggtctct tggtcttgcc tcatctttct gatgaagagg
    11581 aagaaagcaa ggattatctc atgcatatcg ccattttgaa tgaagtaacg ctagagttgg
    11641 aaaaagagtt ggaagacacc agaaataaac ttcccctcta tgcggctatt gacaattatc
    11701 atggacgtat tgaaaatctc attttaagcc aagaaaacca ggatgatcaa gaagattggg
    11761 ctgctttgaa actcttgatt cttagtattg aaagtgatgg tttggaacag gcctatgaag
    11821 aggggaacat tagcaatcgt gcttaccgag tttaccaacg ttatctgaaa aatatagaac
    11881 aaggaatcaa tcgtaaactt gcctcaagac tgacctatta ttttcttgtt tccttgagga
    11941 ttttacgttt tctccttcat gaagttttta ctcttggaaa gaccttccgt agctggaagg
    12001 acaaggagca aagccgtctc cgtgctcttg attatgacca aattgcagag ctctatcttg
    12061 ccaatacaga gatgattatt gaaagtttgg aaaacctgaa gggagtctac agacgctctt
    12121 tgattagttt tatgcaggag tctcgtcttc gagaaacagc tattatcagc agtggtgcct
    12181 ttgtcgaacg ggttatcaat cgtgtcaaac ccaacaatat cgatgaaatg ctgagaggct
    12241 attatctgga gcgcaagttg attttcgaat acgaagaaaa acgattgatt acgactaagt
    12301 atgccaagaa attacgacaa aatgtaaata acttagagaa ctattccttg aaggaagctg
    12361 ccaataccct gccgtatgat atggtggaat tggtaagaag aaattagtta atactcttcg
    12421 aaaatctctt caaaccacgt cagcgtcgcc ttggattata tatgtgactg acttcgtcag
    12481 tttcatctac aacctcaaag cagggctttg agcaacctgc ggctagcttc ctagtttgct
    12541 ctttgatttt cattgagtat aagattataa atgaaggagt atgacatgaa aaagtggtgg
    12601 aaagagctga tagacaagcc tttattaaaa gcttttttgc attattatca agcatcagat
    12661 agtgagttga ccagtgtcgc agtagcctac tattggttga tttcgatttt ccccctgctt
    12721 ttggtggtgg tcaatatcct cccctatttt cagattcctg tgggagaatt tctgtgcttt
    12781 atgaaagacg ttctgccccc aagcctctat gaaggtgtgg aaaaaatagc cagagaagtt
    12841 ttgacgcaac cttcaacagg tttattgagt ttttctgttt tatcggcgtt atggagtttt
    12901 tcaaaatcta tgaatttctt gcaaaaagct tttaacaagg cttatggcgt cgaaaagagt
    12961 cgtggactta tttcccatca gatgctaagt ctcctagtca gttttggctt gcagctcctc
    13021 tttgcgtttg ccttgttttt gatcttattt ggtcagatga ttttggcttt acttgctcat
    13081 tattggacaa aagatggcat tatctatcag gctttacaag ggttggcagg tcctctgatt
    13141 tacgccctcc tctttgctat cttggtcatg ctttattatt ttcttcccaa tctatctaac
    13201 aggaaaatta gctataccct accaggcagt gcctttgttc tcttagtgat tttaggttta
    13261 ctaactctgt tctctagtta tctcaattac tatgtccatc atttggtgga tgtccgaatt
    13321 ttaggttccg ttcttcttgt tgttatgatg ttttggttta tcttgattgc taaaattgtt
    13381 atcctaggcg cggttatcaa tgccagtatg cagagtttga aagatccggt ctttaaaaaa
    13441 gactaactat ttatccatta caaaagcgca tagtatcaaa actttataga accttgatac
    13501 tatgcgcttt tgtgctttga cagttttttg tatagttttt gtccaagatt tctaaatcat
    13561 ctgattaaat tcctattttc ccctatcatt gtccctgttt tttcgaaaat ggaaggagta
    13621 taataaatct atcaataaaa tgataggagg aactttatgg gtcatatttt cttttttcta
    13681 agtgtctttt tggcagggat tctatccttc ttttctcctt gtatcttacc tttgttaccg
    13741 gtctatacag gagtgttact agatgataag gatggtgctc aggcttctag cggcaaattt
    13801 tcaatctcag ttactagttt attacgaact ctggccttta tagcaggaat ttcctttata
    13861 tttattttgt tgggctatgg agctggtttt ttaggcgatt tgctttatgc ttcttggttc
    13921 caatatctta ctggggcaat tattatcctt cttggtttgc accaaatgga gattctacac
    13981 tttaaggggc tttataagga aaagaggcta caactgcaag gacaggggca aaatggtaag
    14041 ggctatagtc aggcattttt attgggcttg acctttagtt ttgcttggac gccttgcgtg
    14101 gggccggttc tggggtctgt tttggccttg gcggcttcag gtggttcagg agcttggcag
    14161 ggagctggtc tcatgttggt gtatacgctg ggcttggcgc taccattctt gcttctagct
    14221 ctgacctcta gttatgtttt gaaacatttc cgaaaacttc atccctatct cggaatcctc
    14281 aaaaaagtgg gtggttttct cattattgtg atgggactct tggttctgtt tggaaatgct
    14341 tcaattttaa gtcaattatt tgaataaaat ggaaaggaat atcaatatga aaaaatggca
    14401 aacatgtgtt cttggagcag gttcgctcct ttgtttgacg gcttgttcag gcaagtccgt
    14461 gactagtgaa caccaaacga aagatgaaat gaagacggag cagacagcta gtaaaacaag
    14521 cgcagctaaa gggaaagagg tggctgattt tgaattgatg ggagtagatg gcaagaccta
    14581 ccgtttatct gattacaagg gcaagaaagt ctatctcaaa ttctgggctt cttggtgttc
    14641 catctgtctg gctagtcttc cagatacgga tgagattgct aaagaagctg gtgatgacta
    14701 tgtggtcttg acagtagtgt caccaggaca taagggagag caatctgaag cggactttaa
    14761 gaattggtat aagggattgg attataaaaa tctcccagtc ctagttgacc catcaggcaa
    14821 acttttggaa acttatggtg tccgttctta cccaacccaa gcctttatag acaaagaagg
    14881 caagctggtc aaaacacatc caggattcat ggaaaaagat gcaattttgc aaactttgaa
    14941 ggaattagcc taggaggtgt cttatgaatg ataagttaaa aatcttcttg ttgctaggag
    15001 tattttttct agccataacc ggtttctatg ttctattgat acgaaatgca gggcagacag
    15061 atgcctcgca aattgaaaag gcggcagtta gccaaggagg aaaagcagtg aaaaaaacag
    15121 aaattagtaa agacgcagac ttgcacgaaa tttatctagc tggaggttgt ttctggggag
    15181 tggaggaata tttctcacgc gttcccgggg tgacggatgc cgtttcaggc tatgcaaatg
    15241 gtagaggaga aacaaccaag tacgaattga ttaaccaaac aggtcatgca gaaaccgtcc
    15301 atgtcaccta tgatgccaag caaatttctc tcaaggaaat cctgcttcac tatttccgca
    15361 ttatcaatcc aaccagcaaa aataaacaag gaaatgatgt ggggacccag taccgtactg
    15421 gtgtttatta cacagatgac aaggatttgg aagtgattaa ccaagtcttt gatgaggtgg
    15481 ctaagaaata cgatcaacct ctagcagttg aaaaggaaaa cttgaagaat tttgtggtgg
    15541 ctgaggatta ccatcaagac tatctcaaga aaaatccaaa tggctactgc catatcaatg
    15601 ttaatcaggc ggcctatcct gtcattgatg ccagcaaata tccaaaacca agtgatgagg
    15661 aattgaaaaa gaccctgtca cctgaggagt atgcagttac ccaggaaaat caaacagaac
    15721 gagctttctc aaaccgttac tgggataaat ttgaatccgg tatctatgtg gatatagcaa
    15781 ctggggaacc tctcttttca tcaaaagaca aatttgagtc tggttgtggc tggcctagtt
    15841 ttacccaacc catcagtcca gatgttgtca cctacaagga agataagtcc tacaatatga
    15901 cgcgtatgga agtgcggagc cgagtaggag attctcacct tgggcatgtc tttacggatg
    15961 gtccacagga caagggcggc ttacgttact gtatcaatag cctctctatc cgctttattc
    16021 ccaaagacca aatggaagaa aaaggctacg cttatttact agattatgtt gattaatact
    16081 cttcgaaaat ctcttcaaac cacgtcagcg tcgccttacc gtatgtaggg tt
//
LOCUS       DAVBCB010000051        14799 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVBCB010000051 DAVBCB010000000
VERSION     DAVBCB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14799
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.051"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..662
                     /locus_tag="VQU28_001643"
     CDS             <1..662
                     /locus_tag="VQU28_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476968.1"
                     /translation="IIRKVQEPEFWKEVNLSDLEKIRLAIRDLLQFLDKTDRKPYYVN
                     FEDRILSTVHETTAFLQVNDLRSYNEKVEHYLKTHLDEESISKLYHNKKLTSDDMLAL
                     EKLLWEKLGSKADYQSHYENKAIPRLVREIIGLDRESANRIFSKFLSDENLNARQISF
                     VKLIVDYIVENGFLETKVLTQEPFKSYGSVQLLFQHQLPVLRNIVQIIELINNRAGEA
                     A"
     gene            complement(1062..2408)
                     /locus_tag="VQU28_001644"
                     /pseudo
     CDS             complement(1062..2408)
                     /locus_tag="VQU28_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
     gene            2470..2664
                     /locus_tag="VQU28_001645"
     CDS             2470..2664
                     /locus_tag="VQU28_001645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476969.1"
                     /translation="MSSKKHPLGEKRNHTGSKLRQGFWMVFRLGVNNWGFKEIENKKP
                     SKNSIAGENEIILNENQRAN"
     gene            2642..2842
                     /locus_tag="VQU28_001646"
     CDS             2642..2842
                     /locus_tag="VQU28_001646"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476970.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDKTDEVSSKHGFEVVDETDEVS
                     NHTYGKATLTRIEEIFEEYKSS"
     gene            complement(2922..3367)
                     /locus_tag="VQU28_001647"
                     /pseudo
     CDS             complement(2922..3367)
                     /locus_tag="VQU28_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547411.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3375..3518
                     /locus_tag="VQU28_001648"
     CDS             3375..3518
                     /locus_tag="VQU28_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476971.1"
                     /translation="MAERFWENLSIILADRNISWIELTRKMFAGEFHYPSELNRLYQK
                     IRH"
     gene            3611..4198
                     /locus_tag="VQU28_001649"
     CDS             3611..4198
                     /locus_tag="VQU28_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2476972.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCSDELTILQILYGASLIS
                     FLWMYGQRKQVIKVNMKTRIQWLGIGFVSLLIISLCFSLIHAQESTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHPGTIIAWCLYV
                     SLGMFLGLVRYKLDLWGSMGLHLVWNLLVYSLLLF"
     gene            4597..5406
                     /locus_tag="VQU28_001650"
     CDS             4597..5406
                     /locus_tag="VQU28_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000926538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2476973.1"
                     /translation="MLLQKELIPMIEANLPNMAYAEKDIAKFFLKQQPLNNYSCKALC
                     ECLNVSKATLTRFAKKCGFKGFRQFIFKYQEMIREKEKLALYTEATEKVLSDYEEMLR
                     KTYTVLDEVQLERIAEMIETAERVYLYGKVSSVLALQEMKMRFMRLGVIGEVLSDEDM
                     ILWNSLLLNENCLVIGASVSGQTDIVLEGLQKAADKGAKTVLMTTRKFDEEDCFFDEL
                     LLLASTNHLSYGNRISPQFPILLITDCLFSHYLESPERQYYYNQTIIHKEE"
     gene            5411..6112
                     /locus_tag="VQU28_001651"
     CDS             5411..6112
                     /locus_tag="VQU28_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2476974.1"
                     /translation="MSQIWTKEKFISQVHGGVIVSCQALPGEPLYNEEFSLMPFMAKA
                     ALEAGAVGIRANSVRDIKAIQKVVDLPIIGIIKRDYPPQEPYITATMKEVDELVECGT
                     TVIAFDATLRPRYDGLVINEFIKKIKEKYPNQLLMADVSNFDEGLYAFKSGVDFVGTT
                     LSGYTSTSVQSDEPDFELMKKLADFNIPVIAEGKIHYPEQLKKAYSLGVTSVVIGGAI
                     TRPKEIAQRFINVIK"
     gene            6137..7054
                     /locus_tag="VQU28_001652"
     CDS             6137..7054
                     /locus_tag="VQU28_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HET2476975.1"
                     /translation="MKDLTKYKGVIPAFYACYDENGEISQERVKSLVQYFIDKGVKGI
                     YVNGSSGECIYQSVEDRKQIIEAVMEVAKGKLTVINHVACNNTKDSIELAKHSESVGV
                     DAIAAIPPIYFKLPEYSIAAYWNAMSEAAPNTDFIIYNIPQLAGVALTGSLYATMRQN
                     PRVIGVKNSSMPVQDIQMFVAAGGEDYIVFNGPDEQYLGGRLMGAEAGIGGTYGVMPD
                     LFLKLESLIQERDLDTAKKLQYAINEVIYKMISGKANMYAVAKEVLRLNEKLDLGSVR
                     QPLEALAEGDLEVAKQAAELIQQARKEFL"
     gene            7082..8623
                     /locus_tag="VQU28_001653"
     CDS             7082..8623
                     /locus_tag="VQU28_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HET2476976.1"
                     /translation="MGTTGFTIIDLIILIVYLLAVLVAGIYFSKKEMKGKEFFKGDGS
                     VPWYVTSVSIFATMLSPISFLGLAGNSYAGSWILWFAQLGMVVAIPLTIRFILPIFAR
                     IDIDTAYDYLDKRFNSKALRIISALLFIIYQLGRMSIIMYLPSAGLSVLTGIDINILI
                     ILMGAVAIVYSYTGGLKSVLWTDFIQGVILISGVVLALFVLMANIKGGFGAVAETLAN
                     GKFLAANEKLFDPNLLSNSIFLIVMGSGFTILSSYASSQDLVQRFTTTQNIKKLNKML
                     FTNGVLSLATATVFYLIGTGLYVFYQVQNAGSAASNIPQDQIFMYFIAYQLPVGITGL
                     ILAAIYAASQSTISTGLNSVATSWTLDIQDVISKNMSDDRRTKIAQFVSLAVGLFSIG
                     VSIVMAHSDIKSAYEWFNSFMGLVLGLLGGVFILGFVSKKANKQGAYAALIVSTIVMV
                     FIKYFLPPTAVSYWAYSLISISVSVVSGYIVSVLTGNKVSAPKYTTIHDIPEIKADSS
                     WEVRH"
     gene            8642..9100
                     /locus_tag="VQU28_001654"
     CDS             8642..9100
                     /locus_tag="VQU28_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2476977.1"
                     /translation="MIISEQSDFRRYASINKHFSKVCDFLENTNLTDLVDGKVDIDGE
                     NVFANCMIYLADGVPGDIFETHKKYLDIHIVVENTEKMAVTSPVRAQSRVPFSEEKDI
                     AFYDSKDYQIVELLPGNMLVTFEEDLHQPKIHCNDEPVKKLVIKVLNEEK"
     gene            9102..11324
                     /locus_tag="VQU28_001655"
     CDS             9102..11324
                     /locus_tag="VQU28_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HET2476978.1"
                     /translation="MKKNIKQYVTLGTAVVLSAFVANSVAAQETETSEVSTPELVQPV
                     APTTPISEVQPKSGNSSEVTVQPRTVETTVKDPSSATEETPVLEKNNVTLTGGGENVT
                     NELKDKFTSGDFTVVIKYNQSSEKGLQALFGISNSKPGQQNSYVDVFLRDNGELGMEA
                     RDTSSNKNHLVSRPASVWGKYKQEAVTNTVAVVADSVKKTYSLYANGTKVVEKKVDNF
                     LNIKDIKGIDYYMLGGVKRAGKTAFGFNGTLENIKFFNSALDEETVKKMTTNAVTGHL
                     IYTANDTTGSNYFRIPVLYTFSNGRVFSSIDARYGGTHDFLNKINIATSYSDDNGKTW
                     TKPKLTLAFDDFAPVPLEWPRDVGGRDLQISGGATYIDSVIVEKNNKQVLMFADVMPA
                     GVSFREATRKDSGYKQIDGNYYLKLKKQGDTDYNYTIRENGTVYDDRTNRPTEFSVDK
                     NFGIKQNGNYLTVEQYSVSFKNNKKTEYRNGTHVHMNIFYKDALFKVVPTNYIAYISS
                     NDHGESWSAPTLLPPIMGLNRNAPYLGPGRGIIESSTGRILIPSYTGKESAFIYSDDN
                     GASWKVKVVPLPSSWSAEAQFVELSPGVIQAYMRTNNGKIAYLTSTDAGTTWSAPEYL
                     KFVSNPSYGTQLSIINYSQLIDGKKAVILSTPNSTNGRKHGQIWIGLINDDNTIDWRY
                     HHDVDYSNYGYSYSTLTELPNHEIGLMFEKFDSWSRNELHMKNVVPYITFKIEDLKKN
                     "
     gene            11364..12473
                     /locus_tag="VQU28_001656"
     CDS             11364..12473
                     /locus_tag="VQU28_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2476979.1"
                     /translation="MVNYGIVGAGYFGADLARSMNKIEDAKVVAVFDPNHGEEVAQEL
                     GSDVCASLDELVAREDIDCVIVASPSYLHREPVVKAAQHGKHVFCEKPIALSYEDCKA
                     MVDACKENNVIFMAGHIMNFFNGVHHAKELITQGKIGKVLYCHAARTGWEEQQPTVSW
                     KKLRSQSGGHLYHHIHELDCIQFIMGGLPEKATMVGGNVYHKGENFGDEDDMLIVNLE
                     YSDDRYAVLEYGNAFRWGEHYVLIQGTEGAIKLDLFNTGGTLRVKGEGESHFLVHETQ
                     EEDDDRTAIYTGRGMDGAIAYGKPGVRCPLWLQTCIDKEMEYLHDIIKGGEITEEYEK
                     LLNGVAALESIATADACTLSVKEDRKVSLSEITNA"
     gene            12630..13520
                     /locus_tag="VQU28_001657"
     CDS             12630..13520
                     /locus_tag="VQU28_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HET2476980.1"
                     /translation="MKIIGIDIGGTTIKADLYNEFGTSLNQFKEIETIIDYDLGTNQI
                     LNQVCDLIGEYILNHSIDGVGISTAGVVNANTGEIIYTGYTIPGYIGVNFTFEIEKRF
                     GLSTFVENDVNCAALGELWKGQAKDKKNVVMVTIGTGIGGSIIVNGQIVNGFNYTAGE
                     VGYIPVGNSDWQSKASTTALIHLYQKKSLKTNQTGRTFFTDLRAGDKVAKETFEIFVE
                     NLTKGLLTITYLLNPEILILGGGILAKKDILLPEIQSSLAKNAMDNRFLPKNLVAATL
                     GNEAGRIGAVKNFLDRISNK"
     gene            13545..13868
                     /locus_tag="VQU28_001658"
     CDS             13545..13868
                     /locus_tag="VQU28_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2476981.1"
                     /translation="MTNSVFSTMQDIENVATDIIKSYDNEIYTYKAVSQEELEKLEKS
                     YDEKSHEELVSIESNLEMKQQNLIDEVNKTIKENYEKIQYISSSRRGEFVEKIIGRVV
                     EKYGY"
     gene            13858..>14799
                     /locus_tag="VQU28_001659"
     CDS             13858..>14799
                     /locus_tag="VQU28_001659"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2476982.1"
                     /translation="MAISQMKRISLLFSKSSLDDVLKTIQELESVQFRDLKVQDNWSE
                     ALEKDEVVFPTIQISHTSNSNHGVIEGNDALIYLMNQQQYLEATVEKLQEYLPKENTF
                     KLLQQPPITTSYEELEKFGKANVAEGVLKKVNRQINRVHELERHIQSNNEEIERLIKW
                     EKLEIIPAHLEQFFFCKGKVGTIPRTVDNRLYNSFLENNIEVQEIFSNDREYGVVVFY
                     QSSCSIDFDEYLFEPFDYSRKELPKQRVVDLDQENMQLITEKENIIASLQDSKNNLID
                     LQWQIDYILSIYARQISKNNLLSTPHLVALEGWIEETR"
BASE COUNT         4960 a         2255 c         2929 g         4655 t
ORIGIN      
        1 aaattatcag gaaagtacag gagcctgaat tttggaaaga agttaacttg tctgatttgg
       61 aaaaaattcg tcttgctatt cgagatttat tacagttttt ggataaaaca gaccgtaaac
      121 cctactatgt taactttgaa gatcgtatac tctccactgt tcacgagacc acagcatttt
      181 tgcaggtcaa cgatcttcgg tcttacaatg aaaaagttga gcattatttg aaaactcatc
      241 tggatgagga gtccatttct aagctatacc ataataaaaa gttgacatct gatgatatgc
      301 ttgcacttga aaaattgctt tgggaaaaat taggtagtaa agcagactac caaagtcatt
      361 atgaaaataa ggcaattccg agattggttc gtgagattat tggcttagat agagagtctg
      421 ccaatcgtat tttctctaaa tttttgtcgg atgagaatct taatgccagg cagatttcat
      481 ttgtaaaatt gattgtagac tacattgtag aaaatggttt tttagagacg aaagtgttaa
      541 cgcaagagcc gtttaaatct tatggttctg ttcaactact cttccaacac caactaccag
      601 tacttcgtaa tattgttcaa atcattgaac ttatcaataa tcgagctgga gaagcggctt
      661 aaattctaaa gtgattgcca tgctgagact catttaaaat taaaaagagt agaaatttat
      721 gctatatatg agaagtttta ttaggaagaa tgtcatcgtt ttcctagaat acagtatcag
      781 ttgttaagtg gttgataaat ttcaaagtag atacttgtac cacgatgttt gttgatcgag
      841 ttattaacaa aagagctact ttgattttaa cccaaatcat tcatacctct ctcaactaga
      901 tgtaacttac aaaacccctg acctcatgag ccactttctt cctcctcatg aggtcagttt
      961 tactttctgc tgttccagta tcgtttttcc tcgctagatt tcctcaaaag ggcagactcc
     1021 tcccttggtg cgtcacacga ttttttcatc tcgactgttc tttaatgcat cattaacgac
     1081 gcttttcttc caggtggttc ataaggaaca ggaagattca ggttgacttt tctaatccta
     1141 gaataaagtg ctgaaaacaa ttcggaatag gcatagagac tagacaattt gaggagctgc
     1201 ttgcgtcctg ttcgaacaca ttttcctacc acgtgaagaa aaagatggcg gaagcgtttg
     1261 attgttaaag tttggaagtc acctccagct agatgtttga gaaaaagata gagattgtag
     1321 gcgatacagc tcatcatcat acgaacttcg tttttgatta aggttgaact atccgtttta
     1381 tcgccaaaaa atccctcctt catctccttg atgaaattct cggcttgacc acgtccacga
     1441 taaagctgaa actggtcttg gcttgttcca ctcgtcatat ttgtaacgag agaaataaca
     1501 tcgtagaaca agtttccttc ttttcgttca gagaactggc agacacgacg cttgtgcgac
     1561 caagatcctg cttgatagag agtttctgag taggcggagt ggggcaagat ggttaagtcc
     1621 tcatcctgtg ggcaagggag ggaaaggtct ccaagacggc tcagaacagt atttttcttg
     1681 agttttatga gttagtattg ccctgttttt tcaattaaat cgtataattt gggggtcgca
     1741 aagccactat ccattcgaaa gagaagttga ttaaaccgtt ctaaaacagg tgtgataaag
     1801 ctgtctgcct cttcagaaca ataacgatta ccaggacgaa gctgggcatt gaaacaataa
     1861 cctgtcttcc cctcgaaagc ataaagagga tgatagccat gagcacgata gtgggcgtta
     1921 taagcaacac cttcttgctt gccataagtt gtgaaatggg tagaatcgat atctacaatg
     1981 agttggttta gctggtgaaa ctgtaaaaag aattcgacca attcaaggtt gaggcatcgc
     2041 aaactatgga ctgtttcctc gtcagttctg gaaagaaaac gggataaggt tggctgtgaa
     2101 gcaagctgcc ctccttccaa caattttgga aagtaggcat cagctgacaa ttctttacaa
     2161 gcatagtccg ttccatagcc tgttaacagt tgaaagagga actggacaag gatatctgaa
     2221 tccgaataac gacagtagcg gcgttggtca ttcgttacta aatacttaga aatccgctct
     2281 tttagtttca actgggaaaa aagttcctga aaaaagataa gaccaccata ctgggttaaa
     2341 tgacctccat cgaaagatag ttggtaaaaa gacttgtttt ggaagtgatg atttggtaaa
     2401 ctgttcatgt gagtttcctt tctttttgtg tttttttcta cacttatacc ataaagggga
     2461 aattcttttt tgtctagtaa aaaacaccca ttgggtgaaa aaagaaacca tacaggatct
     2521 aagctaaggc aaggattctg gatggttttt agattagggg tgaataattg gggttttaaa
     2581 gaaatagaaa acaaaaagcc gagcaagaat tcaattgcag gagaaaatga aataatactc
     2641 aatgaaaatc aaagagcaaa ctaggaaact agctgcaggc tgctcaaaac actgttttga
     2701 ggttgtagat aagactgacg aagtcagctc aaaacatggt tttgaggttg tagatgaaac
     2761 tgacgaagtc agtaaccata cctacggcaa ggcgacgttg acgcggattg aagagatttt
     2821 cgaagagtat aaatcttcct aggataaagc aaagagcctg ggacaaaata gttctcgacc
     2881 acaaaaaagc tagagatttc caattgtgga actttagctt tttaattttg agtcgttctc
     2941 ttattgtatt ttaggaggtt attggccata agtaccaatc ccatgtcaat tctcacttga
     3001 cgcttgcccc tcagattaca tctcttgtaa cccaaacaag cctttatctg cccaaagaca
     3061 ggtgacattc tttagctttc aagttttgat agccttcgtt catatacagt cccttttgag
     3121 gggctgattc aggttcgtcg gcgtagtaaa ccttgatttc ttgtttaaag tctgtctgtg
     3181 ttttctgatg tttggtatgg tgaaaacgat aataccagcc atcaggatgt gtatagctat
     3241 cctccttgtc attatagtac caattcgcta agtttctagc tgactgttta tagcctctct
     3301 tctgttcctt atcaaacatg gcatatttaa tcagatggtt tacctccttt tcatctaact
     3361 aaaggagaag caatatggct gaacgatttt gggagaactt gtccattatt ttggctgaca
     3421 gaaatatcag ctggattgag ttaaccagaa aaatgtttgc gggagagttt cactatccaa
     3481 gtgaattgaa tcgtttgtat caaaagattc gccactagaa gatggaacaa cgaatgcctc
     3541 aaagtccatg ggtagaaagg attgtgcagg tattagattt agattatgaa gatttatttc
     3601 ggaggtaatt atgaaaagaa taattccagt ttatatattc caacaagtaa atgtcctatt
     3661 ggtatctcta tacttattga aatttctttg tagcgatgag ttaactatac tacagattct
     3721 ctatggagcg tcactcattt cttttttatg gatgtatggt caacgaaaac aagtgatcaa
     3781 ggttaatatg aaaactagga tacaatggct tggtattgga ttcgttagcc tactgattat
     3841 aagtctatgt tttagtctaa tccatgcgca agaaagcacg aatcaagcaa acttaattgg
     3901 acttcaacat caaattcctt ggttttcatt tttattgttt ttaatcaatg cgagtatggt
     3961 tgaagaattt ctgtatagag aaattttatg gaacttggtt agaaaattag atattcgagt
     4021 tgctttgaca agtgttttat ttgccttagc acatcatcca ggaaccatta tagcttggtg
     4081 tttgtatgta tcgctgggga tgtttttagg gcttgtacgc tacaaattgg acttatgggg
     4141 cagtatgggg ctacatttgg tgtggaacct actagtttat agtttgctgc ttttttaaaa
     4201 taagtgatct gatttatctc ataaatcagt agtttttata ggattatttt taattcggaa
     4261 acaaaaatgt attaacagca taaaataaag aaactaggaa catttttttt attttttaga
     4321 aataaaatgt tgtatagaaa ttccgaaaga aataataaaa tcaaaataaa aattaataaa
     4381 ataggtatat ttgaacatat tgacgtcgac aaatgttctt gcaagacata aaatacaaat
     4441 aaaaagaata aaaaattttt atatttttat atttgttgtt ctgttataaa atgaaagaaa
     4501 ttagaaacta gtttctaaat catgaaaaat attgaaaaaa aaaaaataag tgttaaaata
     4561 aagaatgtaa acgtttactt caataaagga gctcatatgt tactgcaaaa agaactaatt
     4621 ccaatgatag aagctaactt accaaatatg gcatatgctg aaaaagacat tgctaaattc
     4681 ttcttaaaac agcaacctct gaataattat tcatgtaagg cattgtgcga atgccttaat
     4741 gtatccaaag caacattgac tcgatttgcg aaaaaatgtg gttttaaagg ttttagacaa
     4801 ttcattttca aataccaaga gatgattcgt gagaaagaaa agttagcatt gtatacagag
     4861 gctacagaaa aagttttatc cgactatgaa gaaatgttga gaaaaactta cacggttctt
     4921 gatgaagttc aacttgagcg tattgctgag atgatagaaa ctgctgagcg tgtatatctt
     4981 tatggtaaag taagttctgt tcttgcttta caagaaatga agatgagatt tatgcgtctc
     5041 ggagtcattg gtgaagtatt atcagacgag gatatgattt tgtggaatag cttattactt
     5101 aatgaaaatt gccttgtcat tggagcatcc gtttcaggtc aaactgatat tgtactagaa
     5161 ggtcttcaaa aagctgcgga taaaggcgct aaaacagttt taatgactac aagaaaattt
     5221 gacgaagaag attgtttctt tgatgaacta ttgttattag cttcgaccaa tcatctctcg
     5281 tatggcaatc gcatatcacc tcagtttcca atacttttaa ttacagactg cttattctct
     5341 cattatctgg aaagtccaga gagacaatat tattacaatc aaactattat ccataaggag
     5401 gaataataaa atgagtcaaa tttggactaa agaaaaattt ataagtcaag tccatggtgg
     5461 agtcattgtt tcttgtcaag ctttacccgg tgaacccctt tataatgaag aatttagctt
     5521 gatgcctttt atggctaaag cagctttaga ggcaggagca gtgggcattc gcgcaaattc
     5581 tgtgcgtgat attaaagcaa ttcagaaagt agtagattta ccaataattg gaattatcaa
     5641 aagagattat ccacctcaag aaccatatat tactgctacg atgaaagaag tagatgaact
     5701 tgtagaatgc ggaacaacag tcattgcatt tgatgcaact ttaagaccaa gatatgatgg
     5761 cttagttatc aatgaattta tcaaaaaaat aaaagaaaaa tatcctaatc aattgttgat
     5821 ggcggatgta agtaattttg atgaaggtct ctatgcattt aaatcaggcg ttgattttgt
     5881 tggtacaaca ttatcagggt acacaagtac aagtgtacaa tcagatgagc ctgattttga
     5941 actaatgaaa aaattggctg attttaatat tccggtaatt gccgaaggaa aaattcatta
     6001 tccagaacaa ttaaaaaaag cttatagttt aggtgttacc agtgttgtca ttggtggagc
     6061 gattacacgt ccaaaagaaa ttgctcagcg atttattaat gtcatcaaat aaaaaacaat
     6121 ataaaggaga aatctgatga aagatttaac taaatacaaa ggcgttattc ctgcatttta
     6181 tgcttgctat gatgaaaatg gtgaaattag ccaagaacgt gtaaaatctc tggtgcaata
     6241 tttcattgac aaaggtgtaa aaggtatcta tgtaaatggt tcttcaggtg aatgtattta
     6301 ccaaagtgta gaagatcgta aacaaattat tgaagctgtt atggaagttg ctaaaggtaa
     6361 attaacagtt atcaaccatg ttgcatgtaa taacacgaaa gatagtattg aattggcaaa
     6421 acattcagaa agtgttggag tcgatgctat tgctgctatc ccacctattt acttcaaatt
     6481 gccagaatat tcaattgcag catattggaa tgcaatgagt gaagctgcgc caaatacaga
     6541 ttttatcatt tataatattc cacaattggc aggggttgcg ttgactggta gtttgtatgc
     6601 aacaatgcgt caaaatcctc gtgtgattgg agttaaaaat tcttctatgc ctgtacaaga
     6661 tattcaaatg tttgtagctg caggtggaga agattacatt gtattcaatg gtccagatga
     6721 acaatatctt ggtggtcgct tgatgggagc agaagctggt attggtggta cttatggcgt
     6781 tatgccagat ttgttcttga aattggaaag tttgattcaa gaacgagatt tagatactgc
     6841 taaaaaactt caatatgcta tcaatgaagt tatctataag atgatatcag gtaaggcaaa
     6901 tatgtatgct gtagcaaaag aggttttgcg tctaaatgaa aaacttgatt taggttctgt
     6961 tcgtcaaccc ttagaagcat tggcagaagg tgacttggaa gttgcaaaac aagcagcaga
     7021 acttattcaa caagcacgaa aagaattttt ataataaata aaatcaatat ggagatatgt
     7081 tatgggcaca acaggattta caataattga cttaattatc ttgattgttt atttacttgc
     7141 ggtgttggtt gcaggtatct atttctctaa aaaagagatg aaaggaaaag agttcttcaa
     7201 aggagatggt tcggttcctt ggtatgttac ttcggtatcc atttttgcca caatgctcag
     7261 tccgatttcc ttcttgggac tcgctggtaa ctcttatgca ggtagctgga ttttatggtt
     7321 tgctcaatta gggatggtag tagctattcc actgacaatt cgttttatct tacctatctt
     7381 tgcacggata gacatcgata cggcatatga ttacttggat aaacgtttta attctaaagc
     7441 acttcgtatt atttcagcac tcttgtttat tatttatcaa ttgggacgta tgtctatcat
     7501 tatgtacctc ccatcagctg gtttatcagt attgacagga attgacatca atattttgat
     7561 tattttgatg ggtgcagttg caattgttta ttcttacact ggtggtctaa aatccgtatt
     7621 atggacagac tttattcaag gtgtgattct gattagtggt gtcgttttag ctttatttgt
     7681 actgatggct aatattaaag gtggctttgg tgcagtagca gaaacattag caaacgggaa
     7741 attccttgct gcaaatgaaa aacttttcga tcctaacttg ctttcaaact ccatcttttt
     7801 aattgtgatg ggttcaggct ttacaatctt gtcttcctat gcttcatctc aagatttggt
     7861 tcaacgtttt actacaacac aaaatattaa gaaacttaat aagatgttgt tcacaaacgg
     7921 tgtcttgtca cttgcaactg caacagtctt ttacttgatt ggtacaggct tgtacgtatt
     7981 ctatcaagta caaaatgcag gtagtgcagc tagcaatatc cctcaagacc aaatctttat
     8041 gtactttatt gcataccagt taccagtagg tatcacaggt ttgattttgg cagcgattta
     8101 tgcagcatct caatcaacta tttcaacagg tttgaactct gttgcaactt catggacatt
     8161 ggatattcaa gatgtcattt ctaaaaatat gtcagacgat cgtcgtacga aaattgcaca
     8221 attcgtatct ctagcagtag gtttattctc aattggtgtt tccattgtca tggctcactc
     8281 agatattaaa tctgcatacg aatggttcaa tagcttcatg ggacttgtac ttggtctact
     8341 tggtggtgta tttattcttg gatttgtttc taaaaaagca aataaacaag gtgcttatgc
     8401 agcgctgatt gtatcaacta tcgtcatggt atttattaaa tacttccttc ctccaacagc
     8461 tgtgagctac tgggcatatt cattgatttc aatctctgta tcagtagttt caggttatat
     8521 tgtatctgtt cttactggaa ataaagtatc tgcacctaaa tatacaacga ttcatgatat
     8581 tccagaaatt aaagcggatt caagttggga agttcgtcac taatagaaaa gagaaaataa
     8641 tatgattatt tctgaacaat ctgattttag acgatatgct tctatcaata aacatttttc
     8701 aaaagtctgt gattttttag aaaatacaaa tttgacagat ttagttgatg gaaaagttga
     8761 tattgacggg gaaaatgttt ttgcaaattg tatgatctat ctagctgatg gagtcccagg
     8821 agatattttt gaaactcata agaaatattt agatattcat attgttgttg aaaatactga
     8881 aaaaatggca gtaacttccc cagttcgtgc ccagtcacgt gtacctttta gcgaagaaaa
     8941 ggatattgct ttttacgata gcaaagacta tcaaattgtt gaattgcttc cagggaatat
     9001 gttggtgact tttgaagaag atcttcacca accaaagatt cattgcaatg atgagcctgt
     9061 taaaaaactt gttattaaag ttttaaacga ggaaaaataa aatgaaaaaa aatattaaac
     9121 aatacgtaac cttaggtact gcagtggtat tatcagcatt tgttgctaac tcagttgcag
     9181 ctcaggagac tgaaacttct gaagtatcaa caccagagtt ggtgcaacct gttgcaccaa
     9241 cgactccgat ttcggaagta caacctaaat cgggtaactc ttcggaagtt actgtacaac
     9301 ctagaacagt cgaaactact gttaaggatc catcttctgc aacggaagaa actcctgtct
     9361 tagaaaaaaa taatgttact ttaacagggg gcggagaaaa tgttactaat gagttaaagg
     9421 ataaatttac tagcggtgac tttactgtag tgattaagta caatcagtct agtgagaaag
     9481 gcttacaagc tctgtttgga atatctaatt ctaaacccgg tcaacaaaat agttatgtag
     9541 atgtgttcct tagagacaat ggtgaattgg gaatggaagc gcgtgatact tcttccaata
     9601 aaaatcacct agtatccaga cctgcttcag tttggggtaa gtacaaacaa gaagctgtga
     9661 ctaacactgt tgcagtagta gcagattcag tcaaaaaaac atattcttta tatgcaaatg
     9721 gtacaaaagt agtagaaaag aaagtggata acttcttaaa catcaaggat attaaaggta
     9781 ttgattacta tatgcttggg ggagtgaaac gtgcaggaaa aacggcgttt ggttttaacg
     9841 gaacactaga aaatatcaaa ttctttaata gtgcattgga tgaagaaact gttaaaaaga
     9901 tgacaacaaa cgctgttact ggacatttaa tttatacggc taatgataca acaggttcta
     9961 actatttccg tattccagtt ctgtatactt ttagcaatgg tcgggtattt tcaagcattg
    10021 acgctcgtta cggcggaact catgattttt tgaataaaat taatattgct acaagttata
    10081 gtgatgataa tggtaagaca tggactaaac caaaattaac attggcattc gatgattttg
    10141 cgccagtacc attagaatgg cctcgtgatg ttggtggacg tgacttacaa atcagcggtg
    10201 gtgcaaccta tattgactct gttattgttg aaaaaaataa caaacaagta ctcatgtttg
    10261 cggatgtgat gcctgctgga gtaagtttta gagaagctac tagaaaagat tcaggttata
    10321 aacaaattga tggtaattat taccttaaat taaagaaaca aggtgatact gattacaatt
    10381 atactattcg tgagaatggt actgtatacg acgatcgtac caacagacca actgaatttt
    10441 cagtagataa aaatttcggt attaaacaaa atggtaatta tttgacagta gagcaatatt
    10501 cggtttcatt taaaaataat aaaaagactg aatatcgtaa tgggactcac gtccatatga
    10561 atatcttcta caaagatgca ttgttcaaag tagtgccaac gaactatatt gcatatattt
    10621 ctagcaatga tcatggcgaa tcttggtctg caccaacttt attacctcct ataatgggac
    10681 ttaatcggaa tgcgccttat ttgggtcctg gacgtggaat cattgaaagc tcaactggac
    10741 gtattcttat tccgtcttac actggtaaag agtctgcatt catttatagt gacgataatg
    10801 gagcatcttg gaaagttaaa gttgtgccac ttccttctag ttggtcagca gaagcacaat
    10861 ttgtagaatt gagtccagga gtaattcaag catacatgcg tacaaataat ggtaaaattg
    10921 catatttaac aagtacagac gcaggtacta cttggagtgc accggaatat ttgaaatttg
    10981 tttcaaatcc aagttatgga acacaattat caatcatcaa ttatagtcaa ctgattgatg
    11041 gtaaaaaggc tgtcatttta agtactccaa actccacaaa tggtcgtaaa cacggacaaa
    11101 tttggattgg tctaattaat gatgataata caattgattg gcgttatcat cacgacgttg
    11161 attatagtaa ctatggatac tcatattcaa cattgacaga gttaccaaat catgaaattg
    11221 gattgatgtt tgaaaaattt gattcatggt ctcgtaatga acttcatatg aaaaatgttg
    11281 taccatatat aacatttaag attgaagatc tgaaaaagaa ttaaagctga aatttgaaaa
    11341 tatataaaaa gaggataaaa attatggtaa attacggtat tgttggagct ggatattttg
    11401 gagctgattt agctcgctca atgaacaaaa ttgaagatgc aaaagtggtt gcggtatttg
    11461 acccaaatca tggagaagaa gttgctcaag agttgggatc agatgtttgt gcaagtttag
    11521 atgaacttgt agcacgtgaa gatattgatt gtgtgatcgt agcttcacct agctaccttc
    11581 accgtgaacc agttgtgaaa gctgctcaac atggcaaaca cgtattttgt gaaaagccaa
    11641 ttgcattgtc ttatgaagat tgtaaagcca tggttgacgc atgtaaagaa aataatgtta
    11701 tctttatggc tggtcacatc atgaacttct ttaacggtgt acaccatgct aaagaattga
    11761 ttactcaagg taaaatcggt aaagttcttt attgccatgc cgctcgtaca ggttgggaag
    11821 aacaacaacc aactgtatca tggaagaaac ttcgttctca atctggagga catttgtacc
    11881 atcatattca tgaattagat tgcattcagt ttatcatggg aggacttcct gaaaaagcga
    11941 caatggtagg aggcaatgta tatcataaag gtgaaaactt tggtgatgaa gatgatatgc
    12001 tcattgtaaa cttagaatac tctgatgatc gttatgctgt tttggaatat ggtaatgctt
    12061 tccgttgggg tgaacactac gtcttgattc aaggaactga aggagctatc aaacttgatt
    12121 tgttcaatac tggaggtact cttcgtgtta aaggtgaagg agaatcacac ttcttagttc
    12181 atgaaactca agaggaagat gatgaccgta cagctatcta taccggtcgt ggtatggatg
    12241 gagcaattgc gtacggtaaa ccaggagtac gttgcccatt atggttgcaa acatgtattg
    12301 ataaagaaat ggaatatcta catgacatca ttaaaggtgg agaaattaca gaagaatatg
    12361 aaaaacttct caatggtgta gctgctttag aatcaatcgc taccgctgat gcatgtactt
    12421 tatcagttaa agaagatcga aaagtaagtc tttcagaaat cacaaatgct taacttttgt
    12481 aaaacagaat agtaaattct tatcattata taatttctaa agttctgtga tacaactcat
    12541 tgaataaaga aatagagatg ggactgggat aatgcccagt cccatttttt atcaaaaagt
    12601 aatgagatca aaaatgtggg agtgatgaaa tgaagattat agggatcgat attggcggaa
    12661 caacaattaa agcagattta tacaatgagt ttggaacgag tttaaatcag ttcaaagaga
    12721 tagaaacaat tattgactat gatttgggaa cgaaccagat attaaatcag gtctgtgatt
    12781 taattggtga gtatatttta aatcattcaa ttgatggtgt tgggatttcc actgctggag
    12841 ttgttaatgc taatacagga gagatcatct atacaggcta tacaatacca gggtatatcg
    12901 gagtaaactt tactttcgaa atagaaaaac gttttgggtt gtctactttt gttgaaaatg
    12961 atgttaattg tgctgcatta ggtgaattgt ggaagggaca agccaaagat aagaaaaatg
    13021 tagtaatggt tactattgga acaggtatag gaggcagtat tattgtcaat ggacaaattg
    13081 ttaacggatt taactatact gctggtgaag taggctatat tcctgtaggt aattcggatt
    13141 ggcaaagcaa agcctcaaca accgcattga ttcatttata tcaaaagaaa agcttgaaaa
    13201 ctaatcaaac tgggcgtact ttctttactg atttaagagc tggagataaa gtcgctaaag
    13261 aaacttttga aatttttgta gaaaatctaa caaaaggttt attaacgatt acttatctac
    13321 ttaatccaga aattctcata ttaggaggtg ggatcctggc taaaaaggat attttgttac
    13381 ctgaaattca aagttcttta gctaaaaatg caatggataa taggtttttg cctaaaaatc
    13441 ttgtggcagc tacattagga aatgaagctg gtcgtatagg agctgtaaaa aatttcttag
    13501 atagaatttc taataaatag tatgaaagat aaggaggtgt cacaatgact aactctgtat
    13561 tttcgacaat gcaagatatt gagaatgttg caaccgatat tataaaatca tatgataatg
    13621 agatttatac ttataaagct gtttcccaag aagaattgga aaaactagaa aaaagttatg
    13681 atgaaaaaag tcacgaagaa ttagtttcaa tagaaagcaa tttagaaatg aaacaacaga
    13741 accttattga tgaggttaat aaaacaatca aggaaaatta tgaaaagatt cagtatattt
    13801 catcaagtag gagaggagaa tttgtagaaa aaattattgg tagggtggta gaaaaatatg
    13861 gctattagtc agatgaaaag aatttctcta ctattttcta aaagtagtct tgatgatgtt
    13921 ttaaaaacta ttcaagaact agagtcagtg cagttccgtg atttaaaggt tcaggataac
    13981 tggtcagaag ctctagaaaa agatgaggtt gtatttccaa ctattcaaat ttctcatact
    14041 tctaattcca atcatggggt tattgaagga aatgatgcct tgatttattt gatgaatcaa
    14101 caacaatatt tagaagcaac tgtagagaaa ttacaagaat acctaccgaa agaaaacacg
    14161 tttaaattat tacagcaacc tccgataacg acctcttatg aagaattaga aaaatttggt
    14221 aaagctaatg ttgctgaggg tgttcttaaa aaagtgaatc gtcaaataaa cagagttcat
    14281 gaattagaaa gacacattca aagtaataat gaggaaatag agagattaat aaaatgggaa
    14341 aaattagaaa ttattcctgc gcatttagaa caattttttt tctgtaaagg aaaagtcgga
    14401 acaattccaa ggactgtaga taatcgctta tacaatagtt ttttagaaaa caatattgaa
    14461 gttcaagaaa tattttctaa tgatagagag tacggtgttg ttgttttcta tcagtccagt
    14521 tgctctattg attttgatga atacttattt gaaccatttg attattctag aaaggaatta
    14581 ccgaagcagc gagtagtaga tttggatcaa gaaaacatgc agttaataac tgaaaaagag
    14641 aatattatcg catcgttgca agattcaaag aataatttga tagatttaca atggcaaatt
    14701 gactatattt tatctatcta tgctcgtcaa atctctaaga ataacctttt gagcactccg
    14761 catctagttg cactagaagg atggatagaa gaaactcgt
//
LOCUS       DAVBCB010000052        14432 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVBCB010000052 DAVBCB010000000
VERSION     DAVBCB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.052"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..2288)
                     /locus_tag="VQU28_001660"
     CDS             complement(<1..2288)
                     /locus_tag="VQU28_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HET2476983.1"
                     /translation="MFKVMRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNDTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYAASVTNVNLQSYAKRRKRSVDSIEQLLASIKAAVFSGNTIVNGAPAINASLNIA
                     KSETKVYTGTGLDSVYHVPIYYKLTVTNDGSKLTFTYTVTYVDPTTRALGNLSKMGHG
                     YSIYNTGTSIQTMLTLGSDLGTPSDVKNYITDKDGVLVRSYNTSTMTTWGSGYTWGNG
                     AQMNGFFAKQGYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFKGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASESASTSASASASTSASASASTSASASASTSA
                     SASASTSASESASTSASASASTSASASASTSASASASTSASASASTSASASASTSASA
                     SASTSASASASTSASESASTSASESASTSASASASTSASASASTSASASASTSASASA
                     STSASASASTSASASASTSASESASTSASASASTSASASASTSASASASTSASESAST
                     SASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSASASASTSA
                     SASASTSASASASTSASESASTSASASASTSASASSSTSASASASTSASASASTSASA
                     SASTSASASASTSASESASTSAS"
     gene            complement(2286..2399)
                     /locus_tag="VQU28_001661"
                     /pseudo
     CDS             complement(2286..2399)
                     /locus_tag="VQU28_001661"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
     gene            complement(2637..3098)
                     /locus_tag="VQU28_001662"
     CDS             complement(2637..3098)
                     /locus_tag="VQU28_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2476984.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(3065..3208)
                     /locus_tag="VQU28_001663"
     CDS             complement(3065..3208)
                     /locus_tag="VQU28_001663"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2476985.1"
                     /translation="MAYSTDFKQGALDSIKEGHSHVEAAKFFGVGVRTLFTWEKKDVN
                     KGT"
     gene            3477..3857
                     /locus_tag="VQU28_001664"
     CDS             3477..3857
                     /locus_tag="VQU28_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HET2476986.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            3956..4177
                     /locus_tag="VQU28_001665"
     CDS             3956..4177
                     /locus_tag="VQU28_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2476987.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            4194..4508
                     /gene="trxA"
                     /locus_tag="VQU28_001666"
     CDS             4194..4508
                     /gene="trxA"
                     /locus_tag="VQU28_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HET2476988.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            4815..5348
                     /gene="queF"
                     /locus_tag="VQU28_001667"
     CDS             4815..5348
                     /gene="queF"
                     /locus_tag="VQU28_001667"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HET2476989.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQHHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            complement(5602..6270)
                     /locus_tag="VQU28_001668"
     CDS             complement(5602..6270)
                     /locus_tag="VQU28_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HET2476990.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLSN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(6381..6935)
                     /locus_tag="VQU28_001669"
     CDS             complement(6381..6935)
                     /locus_tag="VQU28_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HET2476991.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRNVLLNQGL
                     HYLSKEKDTRGFSSQYGWVHAFAHGADLLTEVVCHPGFPKNRVHEVFEILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLAQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(7181..8977)
                     /gene="pepF"
                     /locus_tag="VQU28_001670"
     CDS             complement(7181..8977)
                     /gene="pepF"
                     /locus_tag="VQU28_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2476992.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(8988..9731)
                     /locus_tag="VQU28_001671"
     CDS             complement(8988..9731)
                     /locus_tag="VQU28_001671"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HET2476993.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFITQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNVVPSIHLFENKADFLAQLDQ
                     FDRILVAYEEAAKEGEAAALIKAVTGLEKGAKLLFIFGPEGGLSPAEIENFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(9733..10683)
                     /locus_tag="VQU28_001672"
     CDS             complement(9733..10683)
                     /locus_tag="VQU28_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HET2476994.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDMTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(10821..11249)
                     /locus_tag="VQU28_001673"
     CDS             complement(10821..11249)
                     /locus_tag="VQU28_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2476995.1"
                     /translation="MELEISDFQGCKIALFCGDKLLTILRDDKDDIPCPNMWELPGGG
                     REGDESPFECARREVYEELGIHLDEDCLLWSKIYPSVIFKGKKSVFMVGQLRQEQFDN
                     IIFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(11230..12318)
                     /locus_tag="VQU28_001674"
     CDS             complement(11230..12318)
                     /locus_tag="VQU28_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HET2476996.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(12337..12807)
                     /locus_tag="VQU28_001675"
     CDS             complement(12337..12807)
                     /locus_tag="VQU28_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HET2476997.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     VLFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(13098..13550)
                     /locus_tag="VQU28_001676"
     CDS             complement(13098..13550)
                     /locus_tag="VQU28_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HET2476998.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(13587..13766)
                     /locus_tag="VQU28_001677"
     CDS             complement(13587..13766)
                     /locus_tag="VQU28_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HET2476999.1"
                     /translation="MPMTQKEMVKLFTAHGWIKTRGGKGSHIKIEKQGERPITILHGE
                     LNKYTERGIGKQAGL"
     gene            complement(13903..14082)
                     /locus_tag="VQU28_001678"
     CDS             complement(13903..14082)
                     /locus_tag="VQU28_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477000.1"
                     /translation="MNLIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFTIDY
                     TDDELLDKEAVFTTH"
     gene            complement(14247..>14432)
                     /locus_tag="VQU28_001679"
     CDS             complement(14247..>14432)
                     /locus_tag="VQU28_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477001.1"
                     /translation="IFTKQIRHWILSDQVISGKRKLFFREDTPKEILDLYENIKSKLD
                     FAYQEVHSNNGLKKYEK"
BASE COUNT         4196 a         3287 c         2765 g         4184 t
ORIGIN      
        1 gacgcactcg ttgaggctga ctcagacgca ctcgttgagg ctgaagctga cgcactcgtt
       61 gatgctgaag ccgaggcact ggttgacgct gaagctgagg cgcttgttga tgctgaggct
      121 gatgcgcttg tgcttgatga agctgaggca cttgttgatg ctgaggctga cgcactcgtt
      181 gatgcagatt cagacgcact tgtgcttgct gaggctgacg cactggttga tgcggaagct
      241 gaggcgcttg tgcttgctga agccgacgca ctcgttgagg ctgaagctga agcgcttgtc
      301 gatgctgagg ctgacgcact tgtcgacgct gaagctgaag cactggttga cgctgaagct
      361 gaggcactgg ttgacgctga agctgacgcg ctggttgagg ctgaggctga ggcgctagta
      421 cttgctgact ctgatgcact ggtacttgct gaggctgacg cacttgtgct tgctgaagcg
      481 gacgcactcg ttgatgctga agctgaagca ctggtacttg ctgactctga tgcactggta
      541 cttgctgagg ctgacgcact tgtgcttgct gaagccgatg cactcgttga tgcggaagct
      601 gatgcacttg ttgatgcgga agctgatgca ctcgttgatg ctgaagctga ggcgctagta
      661 cttgctgagg ctgaggcact ggttgatgcc gattcagatg cactggttga ggccgattca
      721 gacgctgagg tacttgctga agctgacgca ctggttgacg ctgaagctga tgcactggtt
      781 gacgctgagg ccgaagcact ggtacttgct gaagccgacg cactggtgct tgctgaagcc
      841 gaggcgctag tgcttgctga agccgacgca ctcgttgatg cggaagctga cgcactcgtt
      901 gatgcagatt cagacgctga ggtacttgct gaagctgagg cactcgttga tgcggaagct
      961 gacgcactcg ttgatgctga ggctgaggca cttgtacttg ctgaggctga tgcgctggtt
     1021 gaggccgatt cagaagctga ggtgcttgct gaggctgagg cgctttgact agcacttact
     1081 gagagtgact tagactgtga aagtgactga ctggtcgtgc tagattcaac tacctttcct
     1141 ctacccttga agtagttagt tctaatgtta tctgttttag cagcgtaagg ggtaaatgta
     1201 aaggatgtat ccgttccagt aattggtaca gtccaagatg atgttaatcc atatccttgc
     1261 ttagcaaaga aaccattcat ttgggcacca tttccccaag tatacccact accccacgtc
     1321 gtcattgtag atgtattata ggatcgaacc agtacaccat ctttgtcagt aatgtagttc
     1381 tttacatctg aaggggtacc aagatcactg ccaagggtta acattgtttg tattgaagta
     1441 cctgtattat agatagaata cccatgaccc atctttgaaa gattaccaag agcccttgtg
     1501 gtaggatcta catacgtaac cgtataggta aaggtcagtt ttgaaccatc atttgtcact
     1561 gtcaatttat agtaaattgg aacatgatat accgaatcta aacctgtacc tgtataaact
     1621 tttgtctcac ttttagcaat gtttagactt gcattaattg caggggcgcc atttacaatc
     1681 gtattgccag aaaaaacagc agcttttata gaagccagca attgctcgat ggaatccact
     1741 gaacgcttgc gtcgcttagc ataagattgg agattgacat ttgtaactga tgctgcataa
     1801 tcactagctg gtttcttacg atcttcttcg accttcttag cagttgcttc tgtagcggca
     1861 gctgtttgtg atcctaccac agtagatgat gcagagatac ttgtcgaagc cgatgtactt
     1921 gctgattctg atgcacttgt actcgctgag gtcgaagcgc tggttgaggc tgactcagat
     1981 gcagatgtac ttgccgactc tgaagctgac aaactagttg aactcgctga attacttgta
     2041 gatatcgtac ctagaactac tgtatcattt gttgccaaag catccgtttt ctctacagtt
     2101 ttttcaagta ctgccgactc atttgtaaat accttcgttt gagttgcaac ggttccactt
     2161 atgacagttc ccgcagcaac tattcccttg aggatatcaa gcccagtaat tgaatgactt
     2221 actttatctt ctaccgtttc ggtcatgacc tgagtagtat caacaccacc acgcatgacc
     2281 ttaaacaagc caaaaagaga agtcgaggcc cgtagccaat gcttccctga tttgattaat
     2341 ttgaaccgag ttactcggtc tgtttctcta tattcaccct tctgacgtct aaaaaatatt
     2401 ttgaaatttc ctttcgagta ttattttcat gtatggatat aaataaatta ccatactata
     2461 attatagaat agggaaccca aaatagcaag aaacttgagg tatttttgct aaaaaaaaaa
     2521 agcattttac tattttatat atatattata agataagttt tttataaaga cacgcaaatg
     2581 agtataaatc agctaatttt tggtttatat tcctattttt atgcataatt attctttcat
     2641 tctctaattt gtgaatcaat atatctgatt agaaaactac cattgagttt ttgctccacc
     2701 tcattagtcc gctcaaaacg ccgtccgcta atcttgtcat agactttctc ccctctagga
     2761 gcccctgcat aaggacgata gaggtagcgg ttgattcccg tttcgtcaat atataggact
     2821 ggtaaatctt ttaggttatc caaaatatca agaaactcag aaactttctc aggcttttgt
     2881 tccctaaaac tggtcgtctt ttttaaaatg accttaatct gctttaaaac tgcccatacg
     2941 gagggcaaag cacaatcaaa acgggccgca atttcccgta aaaaagcgtc tggatgagcc
     3001 tctacaaagg ctttcaattc ttctaaaggg atcttacgct ttttgacgac tcgctttttc
     3061 cgctctaagt tcccttgttt acgtctttct tttcccacgt gaagagagtt ctgacgccaa
     3121 caccaaaaaa cttggctgcc tcgacatgac tgtgcccctc tttgatggaa tctaatgctc
     3181 cttgtttaaa atcagtacta tatgccatag ctttattaga ccatgcttat tttaaactaa
     3241 gcaactatag atagagaaat gaaataaaat ctaaattcga atgtgataag gagttagaaa
     3301 ttgaaacaat aggattcccc cattattcac acaaaaataa aaccactaaa aaacagagct
     3361 tgccagcatt cttgaaaagt agtataatta ttgcatgcgc aatcatcttg tgacgcagag
     3421 actgcccgtt aatggacttt cttctatatt atgactctaa aaagaaagga gacactatga
     3481 cctatttgga aaaatggttt gacttcaatc ggcgtcagaa agaaattgaa agtctcttgg
     3541 aagagaccat tgcccaacag agcgagcaaa gtctgacctt gaaagagttc tacctgctct
     3601 actatctgga cttggctgaa gaaaaatctc tacgccagat tgacctgcca gataaacttc
     3661 atctgagccc gagcgcagtt tctaggatgg tggctcgctt ggaagctaaa aattgcggtc
     3721 tactcagtcg catgtgttgc catcaagata gacgctctag ttttatctgc ctgacaaatg
     3781 atgggcaaaa gacactggcc tcgctacaaa agactgtcga agaaagcttg gaagcaggtt
     3841 tggatttctt gatttaagat gattttaaaa aaagttgcgt gcgcaatcat ttttcttgac
     3901 attctctttt ataaggagta aaataaagtt agcattaaac aaaggagttt taaatatgat
     3961 tgaaattacc tatatagatg ccagcaagaa cgaaagaact gtgactttcg agtcttatga
     4021 agactttgaa cgttcacaac aagcttgcct tatcggcgtc gcagactact accctgtcca
     4081 aaaattaact tacaagggtc ataatttgga ctaccatggg acttatggag atatcttctt
     4141 ctatctcatg aaacaagatt taagccaata taactaaaaa aaggagaaat acaatggcaa
     4201 aagcaattac agatgcaaca ttcgaacaag aaacaaaaga cggtttggtc ttagtagact
     4261 tctgggcaac ttggtgtggt ccatgtcgta tgcaaggtcc aatcttggac aaattgtctg
     4321 aagaactttc agaagatgtc ttgaaaatcg ttaaaatgga cgttgatgaa aatccaaaca
     4381 cagctcgtgc ttttggaatc atgtctattc caactcttct cttcaaaaaa gacggccaag
     4441 ttgtcaaaca agttgcaggt gttcacacag cagaacaaat caaggccatc attgctgaat
     4501 tgagctaatc aaacgagaga ccaagtactt tctttggtct cttttttctt gccctttgcc
     4561 atttttcaaa aaatatgcta gactatagta gattgaaact agaatagtac acctctactt
     4621 ctaaaacatt gttaaaaatc gatttgactg tcctgatcga tttgtcctgt ttttatttca
     4681 ttttactata taagtagaat attacgatgt ttgggatatg ccatcgctaa aaaaaaactc
     4741 gatttggtat tttttagttc cctaatggga gctttttgcg tgctctgaac atttcccatt
     4801 ttggaaggag tactatgaaa catcaatcag ccttggtcgt ctttagtggc ggtcaagatt
     4861 caacaacctg ccttttctgg accatgcaac actatgaaac agtcgaagct gtcacctttg
     4921 cctacggcca acatcatcac ctcgaaattc aaattactag agaaatcgct aaggaacagg
     4981 gcattcgtca ccatatcctc gatatgtctc tgctgggaca aatcactgct cagccagact
     5041 ttgcgacgat tcatatttcc tacattcctg acaagctctg tgtcgagtca aaatccctca
     5101 aactatatct atttagctac cgaaaccacg gagatttcca cgaaaactgt atcaacacca
     5161 tcgggaaaga cttggtcaac ttgctagacc ctcgctattt agaagtctgg ggaaaattca
     5221 ctccgcgcgg tggcatttca atcgacccct actataacta cggtaagcaa ggaactaagt
     5281 atgagggctt ggcagaacaa cgcctcttcc aacacgacct ttatccagag aaaattgaca
     5341 accgctaaag tcatactcaa tgaaaatcaa agagcaaact aggaagttag ccgcaggtta
     5401 ctcaaagcag tgtttgaggt tgtagataaa actgacgaag tcagatcaaa acactgtttt
     5461 gaggttgtgg atagaactga cgtagtcagt aaccatacct acggcaaggt aaagctgacg
     5521 tggtttgaag agattttcga agagtatcat acgaaaaagc cctgttcctc aagatgagga
     5581 gcaaggcttt ttgagtttca attattcttc tgttccaagg aaattttttg caacaagggc
     5641 tgcaagaact ccaccagcga ttggtgcaag gatgaaaatc caaacttgtt gaagggctgc
     5701 gccgcctacc aagacagctg gtgccaagct acgagctggg tttactgaaa gtccagtaat
     5761 cttcaatccg acaagaatca tcgccatcaa tgacaaaccg attaccaaac cagcaatcgc
     5821 gccattgccc ttgctttctg aagtcacagt catgataacc aatacaaaca agaaagttgc
     5881 gatgacttca aacaagaaac caccaaagac agtgacaccg tttgccaagg cattttcacc
     5941 aagactagca gttgacatac ctgaattact caagaggaag aagactgcgc cagaagcgat
     6001 gaaagctcca acaacctgac caaggatgta gtttacaagt tctgaagatg acaaacgttt
     6061 gtttacaaac atagcaatcg aaacagccgg gttcaagtga gcacctgaaa cagttccgat
     6121 tgagtaggct gccaccacga ttgccaaacc aaaggcaaag gcgattccaa ggtgaccaag
     6181 gccatcaaga ccatttccaa aaacaacagc tcctgttcca acgaacacaa gcatgaacgt
     6241 accgattaac tcagcaacaa attttttcat tttctttctc cttttttcaa aaactagata
     6301 ctagtctatc aaaagtagga aagggtttca agaaaattga ttggaaattt tttgaaaatc
     6361 atagaactat tagctaatct ctagtattga aaagactgga tagcttcttt caactcatct
     6421 tgtaaactat ttctctggtc aagttggaca tagacttcca ccagacagga tctaaagttg
     6481 gaaaatttgt aaaaatcctc cctttcttct atcggaaaat caacagtttt tatccaagaa
     6541 gctacttgtt cttgcgctaa cttcccttgt aaaataggtt catagatcac tcttgctaaa
     6601 cgccaatcct catcatctgt aaagcgaatc gacattcttt taaataattg gccaagtatc
     6661 tcaaatactt catgaactct gtttttagga aagcccggat gacaaaccac ctctgtcagt
     6721 aaatcggctc catgtgcaaa agcatgaacc caaccatact gacttgaaaa accccttgta
     6781 tccttttctt ttgaaagata gtgcaagcct tgatttaaaa ggacattacg aatttctgga
     6841 gaaggatttc ccaaatgatc aaacaaccac tggatttctt cctggttata atttggtttt
     6901 tcttctgcta tttttcttag taaatcttga tacatggtca atacctctac atttctagca
     6961 actgttcaaa aaggcagtct taaatgactc aatattgaat tctcaattaa atacaatctg
     7021 atataaaatg acgtaaataa ctatcaatac cagttctaca gtaagttcaa atttaacatc
     7081 acgatcttca acgacatttt tgaaaatagc tacaactaag acaaatagaa tgacgcttaa
     7141 caagcccata aacatcattc taaaaaattt ttctattccc ctactctccc aactcagcac
     7201 tataggagat aatctggtca actgtgtcag acaagaattg gatggtatca cggagtggtt
     7261 tgtctgttga aatatcagca ccgataatca tggctgactc aagtggtgtc ttgctaccac
     7321 ctgatttgag gagattgagc cagtcttcag ctccagtttc agaatgtttt agatgaaggt
     7381 aaccagcagt cgagataact agtcctgctg agtaagtgta actatacaag cccatatagt
     7441 agtgagcttg gcgcatccaa gtcagagttg catcatcgtc aatttcaata gcatctcccc
     7501 agaaatccgt caaaacttcc ttcataatgc tgttgagctt gcttgctcca aaggtctccc
     7561 cttcttcaat caatgtatac accttacgct ggaaggcggc ttccaagagg tgggtgataa
     7621 agttatggaa gtaggtgtct gtcaagcgat gagccagagc gaagcgtttt tgacgtgggt
     7681 cattagactg gttctccaag taatcactga gtagcaattc attgaaggtt gacggtgctt
     7741 caacatagta ggtcgacata tgggcattga agtaactttg atgattgtct gaaaagatga
     7801 attgaccaga atgcccgatt tcatgaatca aggtatagac atcgctcaaa cggcctgtcc
     7861 agctcatgag tacataaggg tgtacgcgat atgggtccgc cgcataacca ccggaatcct
     7921 tgccactgtt agcagcaaag tccacccagc gctcttcttg gtaacgagca acttcctgac
     7981 aatattcttg ccccaaaggt tctaccgact tcatgaccaa atcataggca tcgtcaatag
     8041 tcacttcagg attcagggcg ctgtccaagt ccaatttcca gtctgcaaag gtcatctttt
     8101 caagaccatt taccttggca acatgcttga ggtatctctg agcgactggt gcaaagtcct
     8161 tcatgatgag gtcaatctgg cggtcaaaca tgacacggtc cacttcttgt tcagctagaa
     8221 gatagtcaaa gacagagtcg tatcccttca tatcagccaa gagtttttca gacttgacct
     8281 gagccagata ggctgctgca gccgtatttt ggtgcttacg aagtccctct gagaaggaac
     8341 ggaaggattt ctcacgaacc tcagcatcct catggttttg gtagaaattc tcataggtca
     8401 caaagctgtt tttgtaggtc ttgccatggg cttcaaagtc agccatttca aaatccccag
     8461 ctcgcatctt agtataaatg tcctgcggac tgtagaaaac ttcaccgaga tttgtcaagg
     8521 ccttctccac atctgcccct aagtagtggg cttttttgat tttagcctga cgaatggcag
     8581 ctgttaaatg tggcaattta cccaaacgat ccaagacttc ctcgtctgct gccaccaagg
     8641 catcgtcaaa aaaggtcaag gctacgctgg catctgtttc aaattccatc ccagcttgag
     8701 caatatttgc aaattcgtca ttgctatagt ccgtcgtctg aggcataaaa ccatagttgc
     8761 caatatggct catctgaatg tagatctgtt ccaattccgc aaaggccttc tcgaaatcct
     8821 caaaagtgtg aagattgccc ttgtaatcac ggctaaactg gttgatgtct tcgcgagctt
     8881 tctcgattgc acgcaagaaa tcctcacggt cttggtatag agctgttaaa tcccaaagtt
     8941 ccttctctgg aaattctgaa cggtgttttt gttccatttt cttcctctta tttctctaat
     9001 tctactaaaa cactaagggc tgataaggcg taaagcggtg ctgtttctgc tcttagaatg
     9061 cgagggccaa gtcctgccaa aacagctccc ttagcttcaa aattttcaat ttccgcaggt
     9121 gacagaccac cttctggacc aaagataaag agcaatttgg ctcctttctc gagtccagta
     9181 actgctttta taagagcagc ggcttctcct tctttggccg cctcttcata ggctactaag
     9241 atgcggtcaa actggtcaag ttgggctaga aagtctgctt tattctcgaa aagatggata
     9301 cttggaacaa cattacgctt actttgctct gctgctccaa gagcaatttt ttctagtttt
     9361 tcaacctttt tacccaattt cttgccatcc cacttagcaa cggaccaatc ggcagggaag
     9421 gcccaaatct ggctggcacc tagttccgtc accttttgag tgataaactc caacttatct
     9481 cccttgggaa atccagatgc gatggtcact tggactggta gttccacatt gtcatttaat
     9541 tcttggacca actcaaactg acgattttcc atatccagca cgcgcgccaa gcgcttgatg
     9601 ccatcatcaa agactaaggt aacctcatcc tcttctttca agcgcataac ctgaaacata
     9661 tgcttgctgg tttccctgtc ctcgatggtg acaggagaga ctgcactccc cttgacaaaa
     9721 tactgttgca tgctagcctc caatcacacc tgaaatatcc ttggtcttct tgaagacgca
     9781 ggcattccat tccccttgaa tcatatgggt ttcaaggaaa aatccagctg actcagccga
     9841 ctggcgcacc atgtcccact tgtccttgat aatgccactc atgatgaggt agccttcatc
     9901 ctttaccaag cgataagcat cctccgtcag atgaatgaga atatccgcca agatattagc
     9961 cacgatgaca tctgcttcaa tctcaactcc cttaagcaag tctcctgctg ctacatggat
    10021 attttccata ccagtgttga gctcaatatt ttcctgagcg acacgaaccg ccacatcatc
    10081 caagtcatag gcgaaaatct ccttggcacc taggagtgag cttgcaatag aaagaacccc
    10141 tgaaccagtc cccacatcta gcaccgtttc gccaccacga agaacctgtt ccaaggcaaa
    10201 aaggctcatc ttggtagttg ggtgggttcc agtaccaaaa gccatgccag gatccagctt
    10261 gataattttt tctcccgcag tcgcctcata gtctgtccaa gagggaacga tggtcaaatc
    10321 atgagtgatg cgagcaggtt catagtattt cttccagttg tctgcccagt cttcctcagc
    10381 caaggcagtc gtacctattt ttaactctcc caaatccata aaatccgtca attctgctaa
    10441 gcgagcctgc aagtccgcct caaccactgt catatccacc gtgtcagggt agtaggctgt
    10501 cactacgatt tcttcttgct gctccacctc tgggaaaatc tcgccaaaac gatccacatt
    10561 tcccatatag tccatgctgt cttcaatcgc gactccttgc gctcccagct caatcaagag
    10621 attggaaacc aactcctctc cctcacgctt cactgtaact tttaactctt gccatgtttc
    10681 cattattaag ataccaagcc cgtaaaacac aaaaccaaaa taggaaattc tctgaagacg
    10741 cttgtgtcta agagaagttt atctttttgg cacagtgttt agggcgggtt cagtttagaa
    10801 atgtaactga accatccttt ctaatcactt acttttaaat aatcttttaa tctctcttgc
    10861 aactgaggca caacttgact ggaactaaga aattcctcaa cattcatcag ctgatagccc
    10921 tgtccttcat ctccgaagat gatattgtca aattgttctt gtcttagctg accaaccata
    10981 aagaccgatt tcttgccttt aaaaattacg ctaggataaa tcttgctcca aagcagacag
    11041 tcttcatcta aatgaattcc cagttcctca taaacttcac gccgagcgca ttcaaaaggg
    11101 ctttcgtccc cttcacggcc accacctggc aactcccaca tgttgggaca agggatatcg
    11161 tccttgtcat cacgcaaaat cgtcagtagc ttatccccac aaaagagggc aatcttgcaa
    11221 ccttggaaat cagaaatttc tagttccatc ttcagttcct tctaacattt ccttttccag
    11281 ctctgctaac aagttttcat aatagcgttt ctcgtccctc aacattcgac tactatccat
    11341 tttctgtcta gcaatcttga gagccttacg agttcgatct acatctttct tcacctttaa
    11401 ttgataccag gcttgtatca cttgaagatt ggacagtttg agagacagaa acgatttgac
    11461 ctgtcgaata ctagcatatt gctccgcttg ctcaaaatct ccttccaaca aggcgatatg
    11521 aagcagggat agttgggcaa ctgtctgcat catcggagta gttgtcctct caagtaatgc
    11581 ttgaaactgc tgtttagcta cttcttcctt cccttccaaa atggaaactt caccttgcat
    11641 acctaataca ccatccgcaa aactccctcg tgcatcctca ggaactgctt gaacaaagtc
    11701 tttcaaatca tattcttgag gagccagcaa agtttgagca gaatgtctca ataccaggta
    11761 ggcgtatttg gtattttcag ggtgttgtag taattcccaa atttttgctc catcggtgat
    11821 gtcgactggc aaaatgttat ttaggaagaa agataaatta agaaaaatcc aagtccctgc
    11881 aaaataccag cttcttgtca aaaatccaaa cactatcgcc aataatatca agccgagatg
    11941 aaccatcaag cctcctgaaa gcatcaggat gattctttga tcgctttcat cctcttttaa
    12001 accaatgtat tgagcaccaa catttttcag aatggctgtt ctactaagat gaaacctgcc
    12061 tgactttttg gtcaaaataa aatgtcctaa tccaaaagcc accagccgat agcctgtcaa
    12121 gtagccacaa aaagcatgac ccagctcatg aagaataaag attaaataca tgcttagaag
    12181 agcgaaggca taaccaaaag taaaggctaa aactgcggaa taccccaact ctgcaaatgc
    12241 gattgttcca caagcaaaag ctagcataat aaagacaaca gctagcacat aaaccaaata
    12301 agtcccaatt ttcttcataa cacctccaac caactcctag tatcttggat aaggataaaa
    12361 ttctccctct tccaagccaa cttttccttc ttcaaagact tcttggttcc attccatgac
    12421 aaattcctct gcttctgggt cttccaaaaa gtccatgagg acatctagcc caacctcagc
    12481 agtatcttta aggaaaagca caaaataagc taaaaattca cgggaaaatc cttttttagg
    12541 caggtaagga ataacagtca aatagtcttc ctcattgact gttgacttag caggattgta
    12601 gaaaaggacc gcttcctcaa aaagaatgtc atctgatgaa acctctccgt cttcatccac
    12661 catctccaca cctgcagcat tttgcgcttc caatagaaaa ctcacttcta ccgcatggtt
    12721 gcgtttgtcc cagctaatct caaagtcaaa gggaaagttc ttgtccaact cttcctctaa
    12781 aacatctaaa aatccgtatg ttgccatttt gtcctctttc tatgcgactc tttaatcgcc
    12841 ccgattgctc ggaaatatgc taaaatagat actaccatct taccacaaaa ttattttatg
    12901 tcctaattat accatattac ctcatttaaa cccttggtat cagtgatttt cttaaaagtc
    12961 tgatttcttc atttctcata aaaatcaata taaaaagccc tcgaaagggc taataaatct
    13021 ataaaatcaa taggcgagta actagcacaa gtggacgtgc ttttttattg actattacca
    13081 cgataccacg cttaatctta ggcttgaact ttcttatctg caatagcgtc tgtcaaagtc
    13141 tgagaaaagt taagtcccat ttctcgtccc aacttatctg cccattttgg tatggtcaaa
    13201 gtctttttaa tgggttcctg acttcctagg tattctgata catcaacaga taccatagaa
    13261 ataaaagatt tatcaaggtc ataggttgac acgaaatctt catcatcttt aaaaggatca
    13321 ttatcaatta aagacaagct attgatatct gatggctgag gtaactctcc atcactctct
    13381 atcaaatctg caacagttat ccctagccac tccgacccca tagccaaagc ctcagaaatc
    13441 ccctctcctt gtgtagctga gtattcaaaa tctgggaaat ggacaaaata agtcgcttct
    13501 gttccgtctg tgtcgtcata ataaaataaa gctggatacg taactaacat ttcactacct
    13561 ccatatcaaa aagcagggac tgaattttac aacccagctt gctttcctat ccctctttca
    13621 gtgtacttat tcagctcacc atgaaggatt gtgataggtc tttccccttg cttctcgatt
    13681 ttaatatggg agcctttacc gcctctagtc tttatccaac catgggccgt aaagagttta
    13741 accatctctt tttgtgtcat aggcatagcg cttttacctc ctgacaacac cattataaca
    13801 cgtgttacac gtattgtaaa ggagtgatac ttattattct attatacata aaagccccta
    13861 gatgtggttc taagggaaac caatttattc agacctattt ttctaatgag tagtaaaaac
    13921 tgcttcttta tcgagcaatt catcatctgt atagtcaatt gtaaaagtat ctcgatctaa
    13981 gacagattga ggcggagttg aatgaatcat aggaccactg cgtactctat attttttatc
    14041 tccaattttt acaaactgat actcttcgaa aatcaaattc aaaccacgtc aacgtcgcct
    14101 taccgtactc aagtacagcc tgcggctagt ttcctagttt gctctttgat tttcattgag
    14161 tatgattaac tctcaagtct tcgaaatcag gattttcaac agttattaca aggaggcgat
    14221 ttactacttc aaaaacatca attattctat ttttcatatt ttttcaaccc attattagaa
    14281 tgaacttctt ggtaagcaaa atcaagttta gatttaatgt tttcgtacaa atctaaaatc
    14341 tcttttggag tatcttcccg gaagaaaagt tttcttttcc ctgaaataac ttgatcacta
    14401 agaatccaat gacgaatttg ttttgtaaaa at
//
LOCUS       DAVBCB010000053        14416 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVBCB010000053 DAVBCB010000000
VERSION     DAVBCB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.053"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(10..1299)
                     /locus_tag="VQU28_001680"
     CDS             complement(10..1299)
                     /locus_tag="VQU28_001680"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HET2477002.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVTLPIENALDVYIA
                     VLGEGANIKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(1377..2624)
                     /locus_tag="VQU28_001681"
     CDS             complement(1377..2624)
                     /locus_tag="VQU28_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HET2477003.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGVSIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTLFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVSVIISSLTGNDYFGKQSLPRLMMWETVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(2815..3665)
                     /gene="rgg"
                     /locus_tag="VQU28_001682"
                     /pseudo
     CDS             complement(2815..3665)
                     /gene="rgg"
                     /locus_tag="VQU28_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398326.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes.; frameshifted;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
     gene            3789..4130
                     /locus_tag="VQU28_001683"
     CDS             3789..4130
                     /locus_tag="VQU28_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HET2477004.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            4188..4268
                     /locus_tag="VQU28_001684"
                     /pseudo
     CDS             4188..4268
                     /locus_tag="VQU28_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(4474..6177)
                     /gene="ilvD"
                     /locus_tag="VQU28_001685"
     CDS             complement(4474..6177)
                     /gene="ilvD"
                     /locus_tag="VQU28_001685"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HET2477005.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(6410..7342)
                     /locus_tag="VQU28_001686"
     CDS             complement(6410..7342)
                     /locus_tag="VQU28_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HET2477006.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSDSDIVVLEADLSSSMATNNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(7339..8196)
                     /locus_tag="VQU28_001687"
     CDS             complement(7339..8196)
                     /locus_tag="VQU28_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HET2477007.1"
                     /translation="MILSKNREDGLRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(8200..9546)
                     /locus_tag="VQU28_001688"
     CDS             complement(8200..9546)
                     /locus_tag="VQU28_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2477008.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFVGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSATEE"
     gene            complement(9559..9843)
                     /locus_tag="VQU28_001689"
     CDS             complement(9559..9843)
                     /locus_tag="VQU28_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HET2477009.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(9847..11877)
                     /locus_tag="VQU28_001690"
     CDS             complement(9847..11877)
                     /locus_tag="VQU28_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HET2477010.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NSHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            12069..13076
                     /locus_tag="VQU28_001691"
     CDS             12069..13076
                     /locus_tag="VQU28_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HET2477011.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKQISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            13079..13279
                     /locus_tag="VQU28_001692"
     CDS             13079..13279
                     /locus_tag="VQU28_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HET2477012.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            13431..13613
                     /gene="rpmF"
                     /locus_tag="VQU28_001693"
     CDS             13431..13613
                     /gene="rpmF"
                     /locus_tag="VQU28_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HET2477013.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            13629..13778
                     /gene="rpmG"
                     /locus_tag="VQU28_001694"
     CDS             13629..13778
                     /gene="rpmG"
                     /locus_tag="VQU28_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2477014.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(14270..>14416)
                     /locus_tag="VQU28_001695"
     CDS             complement(14270..>14416)
                     /locus_tag="VQU28_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HET2477015.1"
                     /translation="YLNESGSMATGWVTVSGKWYYTYNSGDLLVNTTTPDGYRVNANG
                     EWVG"
BASE COUNT         4645 a         3043 c         2488 g         4240 t
ORIGIN      
        1 ctttgatttt tattgagtat aaaatcctag tttttcaaag atttctgaga agttttggct
       61 gattgtctca agtgacactt gcacttcttc tcgggtttgg ttgttcttga ccgtcacttg
      121 tccgctttcg acttcgctct ctcctagggt gatgagggtc ttagccgcaa agacatcggc
      181 tgacttgaac tgagctttta gtttacggtt gaggtaatca cgctctgctt tgaaaccttg
      241 ttggcgaaga gcctgtacca attccaaggc cttgatattt gccccttcgc ccaagactgc
      301 gatatagaca tctagggcgt tttcgatagg gagggtcaca ccttgctttt caaggatgag
      361 aagcaggcgc tctacaccaa gtccaaaacc aaatccagca gtttcagggc ctccaaagta
      421 agcaaccaaa ccatcgtagc gaccacccgc acagacggtc aggtcattgc cctcaatctc
      481 tgtgataaac tcgaaaatgg tgtggttgta gtagtccaga ccacgcacca tattggtatc
      541 gatgatgtaa tctactccaa gattttccaa catctgacgc acagcatcaa aatgagcttg
      601 gctttcttca tcaagaaagt ccaagataga cggcgcattc tctactgcca ccttgtcttc
      661 tttttcctta gagtccaaga cacgaagagg attttcctcc aagcgacgtt ggctatcctt
      721 agacaaggtc tccttgagcg gtgtcaaata gtcaatcaag gcttggcggt aggctgcacg
      781 gctctcagga tttccaagag tgttgaggtg caatttgaca ccttgaatac cgatttcctt
      841 caaaaaatgg gctgccatag cgattgtttc cacatcggta gctggattgc tagagccaaa
      901 acactcaaca ccaatctggt ggaattggcg caagcgccct gcctgtggac gctcataacg
      961 gaacataggt cccatgtagt agaacttgct tggcttttgc acttctgggg cgaaaagttt
     1021 attttccaca taggaacgga caacgggtgc agttccttct ggacggaggg taatatgacg
     1081 gtcacccttg tcataaaaat cgtacatttc cttggttacg atatccgttg tatctccgac
     1141 agagcgactg ataacctcgt aatgctcaaa aataggcgtg cgcacttctg catagttgta
     1201 gcgtttgaaa atctcacggg caaagccctc aacgtactgc cacttagcag actcagcagg
     1261 taaaatatcc tgcgttcctt ttggtttttg taatttcata gggaatcctc tttaaactta
     1321 atagtcttat tttaccataa atagagggat taaaacagta agaaaaaaat taggatttag
     1381 atatcatttt tgagattaag aattgtcaaa aaaatagcta gcaaggaaag accaacaaat
     1441 agcatccaag tcaactgtat attccatacg gctactagtg aaaaacaagc tgttcccaca
     1501 ggtatggata aggtaaacaa tagacctaaa aaattactag tacgagctag aacctctgga
     1561 gctagatttt tcatgagcat ggcactaatc tttggttgaa ctttaccaga cacatacaga
     1621 gtaaagaaga gaaatagcaa accaagcacg acttgattga ataaattagc caaaccaact
     1681 agactaagtc ctacggtctc ccacatcatc aatctaggca aggactgctt cccaaaataa
     1741 tcattgcccg taaggctact gatgatgact gatactaaaa cacagaattg attgataaat
     1801 agtgcctctg tataagaaaa attcaagaga gaatggctca aaaagaagat attataaatt
     1861 ccacccaaag cgccacccaa ggaattaata agcaagacag caaagagcat aaaaccaaag
     1921 tttttctgtc cacttttaag aaaaacgaga cgtaaatttc ggtaaattgt taggaactgg
     1981 tctttgatag aaagcttctc attttttaag ttttcaccat cagcagatga cattgacagg
     2041 ctcaatttgc tttttcctaa aaagaggata gtggctgata ctaggaaaaa gcaggcattg
     2101 attcccgcaa cgagagaaaa attgttgacc gatagagcta agagccagac tccgaaagct
     2161 tgaccaccaa tagctgaaat ataggtgatg aactgtgaaa aagaataagc ctccatcaga
     2221 tcatcttcag ctactttttc cttaataaga ggcatacgca ggccacctgc aaaatcactg
     2281 atgatatcac taatgacatt gatcaaacac aggctagaaa aggcaaagag actagcttgc
     2341 tgaacaacta gggctgctag aaaaaataga accgcctgaa acaaaccgct atagaccatc
     2401 catttgacct tgtccctcgt gtaatctgcc cgaatccctg caaaaactgt aaagagggtc
     2461 ggaagaatca tgacaatatt cgccatagca acagcaaaag atgcttgtga caaggtcgat
     2521 gcatagacga taaagaccag gttgaaaatc gaaacaccaa aagcattgaa gaagcgtgat
     2581 aaagtcaaaa ttctataagc tggatttcta aacaatagtt tcatagataa tctccctcct
     2641 tgttctaagt tctacatcaa gaatagcttt agtatagcac ttatgaattt gcgaaacgat
     2701 tcttttgaat atatgcaaca aacaaaatga gcaaaaaaac gatagaactt ttgtaatgac
     2761 ctgagagagg gcaacagttc tatcgcttca aattttttct tggtttgcag atattcaaga
     2821 atcgggagtt tttctatagt attcggcaga tttattacag ccaagcatct caaaaatacg
     2881 gacagcatcc tccatctttt tctggccttc cttgactcta ccttgcttgc tatcaaggag
     2941 accttctgcc cacagataaa caattcggaa ataggtctca ttttccttgt agaaatgctc
     3001 ttcgataaca cgtttaaaat aataggcatt ggtaaattct tcacactcaa tactagctaa
     3061 aaagccattc aatagtatag tatgaaaaag gtttcgattg ccagacattt ccattagaaa
     3121 atcggattta cgtaccattt ctcgtacata tctagtaaaa agagaaacag ataaaaatgg
     3181 agaactgact gaaaataaat tgagttcata gattccccag atctcggtag aaaacaaata
     3241 atcatgaagg acttttcctt cctctgctgt taagtctacc ctttcatcta tgctcttcat
     3301 ataagacttg ataataatgg catttagaat atgtttctgt ttgttgtgag aatgggcatg
     3361 cttttatact ccctgcgata taagtcctca agaggtgcta tattctttgg ttccaagaca
     3421 tctgtaattt cttttctcaa ctcagaatct gtatcatact ggaaacctct tgccagaaag
     3481 aggatctcct ccacactggc agatatattt tccagagcaa atagaaactt ttccaccgaa
     3541 agctcactct gacctgtttc aaaacgggac aacatagacg gcgaaaattg tcctccggtt
     3601 gcttgtctca gtgagatatt tcttgactct cgtaattgtc taaagacttt tccaatctgc
     3661 tccatagact tccccttgat tccgtatttt cttcatttta tcatattttt cagaaaattc
     3721 atcaaaaact tgccaaattg tcagaattat gagaaaatag aggatattta tcacgtggag
     3781 ggactgctat gagagacgat atcaaaatca atgaccgtgc tttggccttg caagaccaaa
     3841 ttatcgaaaa actagagaaa gtttttgata cagatgtgga attggatgtt tacaatctag
     3901 gtctgattta tgaaatcaat ctggatgaaa cggggctctg caagattgtc atgaccttca
     3961 ccgatactgc ctgtgattgc gccgaaagcc tgcctattga aatcgtggca ggtctgaaac
     4021 aaatcgaggg tatcaaagat atcaaggttg aagttacctg gtcgcctgct tggaaaatca
     4081 cacgaatcag tcgctatggc cgtattgccc ttggactacc acctcgttaa gcagaccaat
     4141 cacttttaaa gatgaaaaaa gcaagccgaa aatggcttgc tttttgatac tcaatgaaaa
     4201 tcaaagggca aactagaaaa ctagccgcag gttgctcaaa acactgtttt gaagttatgg
     4261 atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga actgacgaag
     4321 tcagctcaaa acactgtttt gaggttgtgg atagaactga cgaagtcagc ccaaaacact
     4381 gttttgaggt tgtggataga actgacgaag tcagtaacca tacctacggc aaggcgacgt
     4441 tgacgtgatt tgaagagatt ttcgagtatg agtttatttt ttacctgact tgtccatatt
     4501 ccagaagtct gtcacggctc cgcgtgaagc agatgatacg atgtgggcat atttaccgag
     4561 gacaccacgg ctgtaaagtg gtggcaaggt tgtttctgcc ttgcgttttt caagttcttc
     4621 ttcggatacg gccatagaaa tttctttggt atcttggtca accgtaacga tatcgccggt
     4681 acggagatag gcaattggtc caccatcctg agcttcagga gcgatatgtc caacaaccag
     4741 accataagta ccaccagaga aacgtccgtc cgtcaagagg gccaccttat ctccctgacc
     4801 tttaccaaca atcattgaag aaagtgatag catctcaggc ataccaggac cacctttagg
     4861 tccaacaaaa cgaacaacga ctacatcgcc atcaacgatt tcatctgtca gaacggcctg
     4921 aatcgcatct tcttctgagt caaagacctt agctggccca acgtgacgac gcactttaac
     4981 acctgatacc ttggcaactg caccgtcagg agcaaggttc ccgttcaaga tgataagcgg
     5041 accatccgca cgttttggat tttcaagtgg catgataact ttttggcctg gagtcaagtc
     5101 tgcaaagtca gccaagtttt cagctacagt cttaccagta catgtgatgc gatctccgtg
     5161 aaggaaacca tttgccaaca aatacttcat aaccgcaggg acaccaccga cttcgtagag
     5221 gtcttggaag acatactgac cagatggttt caagtcggcc aagtgaggca cacgttcttg
     5281 aatcgtattg aagtcctcaa gtgacaagtc aacatttgcg gcatgggcaa tggcgagcaa
     5341 gtgaagagtg gcgtttgtag aaccaccgag agccatcgtt acagtgatag catcttcaaa
     5401 ggcttcacga gtcaagatat ctgatggttt gagaccaagt tccaacatct taacaacagc
     5461 acgtcctgct gcttcgatat cttctttctt atcagctgat tcagctgggt gagaggatga
     5521 ccctggcaaa ctcatcccta gaacttcgat agcagttgcc atggtattag cagtatacat
     5581 accaccacaa ccaccagggc cagggcaggc attacattca agacgtttca cgtcctcagc
     5641 tgtcatgtca ccgtggttcc attttccgat accttcaaag acagaaacca agtcgatatc
     5701 tttaccatca agatttcccg gtgcaatagt tccaccatag gcgaaaatag ctgggatatc
     5761 catattagca atagcaatca tagatccagg catgttcttg tcacagccac cgatagcgac
     5821 gaaggcatcc acgttgtgac cactcatagc cgcctcgatg gagtccgcga tgatgtcacg
     5881 agatgttaga gagaaacgca taccaggcgt tcccatagcg atcccgtccg ctacggtaat
     5941 ggttccaaac tgtacaggcc aagcgcctgc agatttgaca ccttctttag ccagtttccc
     6001 gaaatcatgc aagtgaatgt tacatggtgt attttccgcc caagtcgaaa tcactcccac
     6061 aatcgatgtt tcaaagtcct tatctgtcat accagtcgca cgaagcatag cacggttagg
     6121 tgatttaacc atgctgtcat aaatgctact gcggtgacgt ttatctaatt cagtcatctt
     6181 atccctccca tttcagtttt tactattata gcacaatttt cgcatgaaga acagaataaa
     6241 attcttgaat tttcagaaaa ttctatacac atgtgaaata tttaaaatta aaaacaacaa
     6301 agcggattag tgcactttct gatgaccaga atatgctttt taatccgctt tctttaaata
     6361 acgtactgta atttttacag aaattctttc aaataagtgt atttaacatc tatcttgcat
     6421 tataaatttc tagaaccttc tcttttatat tcgattcact caaaccatac tcattaagaa
     6481 gataatccat tttccctact tgaccgaatc tttcttgaac acccatccga tgaatttttg
     6541 ttattccatc atcagagaat aattcacata aagcactgcc aattccacct atctgattgt
     6601 ggttttctac agtaaatata gtttttccac ttaacattgt ttttatctgt tctggtatcg
     6661 gtttgattct aaataaatct atcacaccta ctgaataacc taatttagac agttcatctg
     6721 caactcgaat acttggagca accattatgc cagaagcaac gattacaaga tcttcaccat
     6781 gccttaactc aatgtagcct ttagaaaaat cttctccacc ttgatacaca ggaactggag
     6841 cttttctaat tgttcgaata tattttagtc cttttaagtc taatgtctgg ttcaatattt
     6901 cacgaaattg gatatcatca gttgcttcga aaatgattga tttaggaatt aaacgtaaca
     6961 atccaatttc ttcaaatggc atatgtgttc caccattcat ctctgccgtt actcctgcat
     7021 ctgatccaat cacagtggca tccaattgtg cgtatccaag agaaataaat aattgatcaa
     7081 atactcttcg tgaagcaaaa ggaccaaatg tatgaagata aggtctaaac ccctgaatag
     7141 acaagcctgc tgcaaggccg accatttctg cttccataat cccaacattc acataacggt
     7201 ctccaaagtc cttttcaaga ttattagtag ccatcgaact tgacaaatcg gcttctaaga
     7261 ctactatatc agaatcactt tgattagcct ctaaaaggaa gtctctatat acatgccgta
     7321 attctttcgt acttctcatc attctgtttc ctccaattcc tgacttaatc tttctacaac
     7381 tgaagttaac atttgtttct cctctacagt agggcgaaga tgatgattgg atttcatttc
     7441 ttccagctct tgaacccctt gacctttaat agtatctaat acaatgcact taggtgatga
     7501 attatttgac tgttttaatt ggacaatccc ttcataaatt tctctaatat ctgaaccctt
     7561 gaccctaatg gattcaaatc caaatgctga aaatttttct acgaaatcac ctggattaca
     7621 aatatccttt gtaaaaccat ctaattgttt tttgttatca tcaacaaata caattaagtt
     7681 ggataactgt tgatgagaag caaactgtat agcctcccaa cattgtccct catttaactc
     7741 accatctcca acaatagcgt aagtataaaa gggactcttt cttattctct gaccatatgc
     7801 aagtccagtt gcaacactaa ttccttgtcc taaagagccc gttgtcatat ctatgcctgg
     7861 cgttagattt ctatcaggat gagacggtaa tttggttcca tttgtattta aagaatataa
     7921 gaattctttg tcaaagaaac cattcaaata gagtgtactg tatagagctg gtcctccgtg
     7981 accttttgat aatatgaaat aatctctatc tcgtgctgca aatatttctg gagtcattgg
     8041 cattatttca ccataaagca ccgctaaaac ttctacgata gacagacttc ctccgtaatg
     8101 tccgaatcca agatgattca atgttctaag agtatttaat cggatgttag tcgcaaattt
     8161 tcttaaccca tcttctctat ttttacttaa aatcatccct tattcctccg ttgcagatgg
     8221 ctttttaata aaggatactc caaacataac tgctagaata agaacaagac caatcacaat
     8281 gcctgcttgt gagccaaatt gatttaacat tcctaaaata attcctgata gaccaaaatc
     8341 tgcatctgag aaagttgatc cttggaaacc aagtcctccc aaaactggca ttaaaaagac
     8401 tggaagaaaa ctgattaaaa taccttgtaa aaatgctcca atagtggctc cacgaacacc
     8461 accagatgca ttcccaatga cacctgcagt cgctccacag aagaaatgag gcacaacacc
     8521 tggtaagata acaaccgttc ctgaagcaat cataattacc atacttacta aaccaccaac
     8581 aaaactagag ataaatccaa ttagaactgc attgggtgca taagtataaa caatcggaca
     8641 atccaaagca ggttttgaat taggtacaag acgctctgaa atacctttaa aggctggaac
     8701 aatttcgccc aaaataaggc gaacacctgc taaaataaca aatacccctg ctgcaaattg
     8761 acctgctaat tgtaaagcat aaactagacc acttgtacca ctactgattt ctttttctat
     8821 atattctgac cctgcaaaga tagctacaat aatgtaaata actgccatgg ataaagtaat
     8881 actaacagta ctatcacgta aaaaagctaa actctttgga aatttaatgt cctctgttga
     8941 ttttgatttg tcaccgataa ggctaccagt aaaaccactc aaccaatatc ccaaagaact
     9001 gaaatgacct aaagctacct tgtcatttcc agttaattga accatatatt tttgcacaaa
     9061 tgctggggaa atactcataa taataccgag tgctaatcct cctagtaaga tgagaggcaa
     9121 gctagtaaag ccagcaactg ataaaatgac cgcaatcata catgccatat atagagtgtg
     9181 gtgccctgtt aaaaaaatat atttaaatcg agtaaaacga gcgattaaga tattgaacac
     9241 catgcctgca aacataatca ttgcagtagc tgagccatat gttgttaaag ctacagctac
     9301 aattgcttca ttattcggca caacgccaga taaatgaaaa gcatgctcaa acatggtacc
     9361 aaatggattt aaagaatttt gtacaattcc tgcaccacca gatacaacta agaaaccaac
     9421 aaaggtctta attccacctt taataatatc aggtaatttc ttcttctgaa gaactaatcc
     9481 taagattgca attaaagcta ctaaaatagc tggtgtacta acaatatcca atatgaactt
     9541 catcatgacg ctagcctcct atataagtcc tttttcttca caaagtttag taattaattc
     9601 tcgtagttca tccatatcaa taatactatt taagatacga acatctccaa gatgactagc
     9661 tgaatcagct agatcacgac caacaatcca aatatcagct gcatttggat ctgctccacc
     9721 taaatcataa tgttcaactt ctacatccga aacattcaaa tcactcaata cagattcaat
     9781 attcatctgt accataaaac ttgaacctaa tcctgaacca caagctgtac caatttttaa
     9841 cattatctaa tcctcctgtt taattatcat tttaatgtca tcatagtttt ttgatgatat
     9901 taaagtttga acatgatttt tatctcttaa aattgttgtt aaatgtgaca aagcctttaa
     9961 atgactctca ttatcaatgg ctgcaataca aatcaacaat cttacctctt gttctggatt
    10021 atccaataaa taaatcggtt cttccaaaac taacattgac attcctattt cattcacacc
    10081 ttcatctggc cgagcgtgag gaattgctac tcccttccct aaattaataa aaggtccaaa
    10141 ctcttctact ttttgaatca ttgcctcagg gtagttctca gttatcttat cttgatccaa
    10201 aagcggttta gctgctaaac gaatcgcctc cttccatcct aatttttgcg aactaacctg
    10261 ataggtttct ttggtaataa gttgttctag cactggtaca atttcctttc tatcattttt
    10321 ttggtaaaga taattcttta acgccaatct taattccaat tcttgtgtaa taattccata
    10381 tcttttgaca atattcagga tttgttcaat ctcaaaatct ccatactcta aattcggaaa
    10441 atcttttaac actagttcta ctagttgtat tgcttgctct tcagtcatca taaccgaaac
    10501 tagataattt ggcttttctg tctccacctt tatggtagaa aaaaccatat catagtcact
    10561 actagctttc acctgtaaat catcaatctt tgaggttcct ataaactcaa tttgaggaaa
    10621 taatgctaat agattctctt ttaacatcaa tgaagaacta acaccattag gacaaatgat
    10681 tgctgcttta taccattttt gaggcaaagt atctgctttc tttaaataac ctccgaaatg
    10741 gataacaaaa tatgctgttt cactatcagg tatgggattg tcaatagcgt ccatcaaggg
    10801 catcaaagaa tctttgacta attcaaataa atcaggataa tgttctttaa catgcaatac
    10861 atattcattt gaactaggta ggccgaactt taatctatag taagccggta taaggtggcg
    10921 gcgaagattt tctctcaatc cttccctttg tttaaaatgt aacaaagaaa tatcttccat
    10981 tctacttata atagcctctg ttaattgatt aaagtaaacc ggagcaacat ctacttcacc
    11041 ttcaaagcaa cttgataata aaacggtgat atagcgataa tcatcctcag aaaacaccgt
    11101 atctataatt cccaaatcaa ccactgtatc caataaaata gtggttatat cttgaataac
    11161 aggagatact aatgtctctg aaagacatac tctttcaaca tccctttgat acctacacag
    11221 aatgaatact aaaccgaaaa ggtaaacttt taattgatta acaataggta ctagctgtag
    11281 cttctcataa taatctttaa ctacctgatc aatcaaatca taagttaatg aataccccca
    11341 actggataaa acataatcca aaccccaaat ccctatggag gattccagca actcactaac
    11401 catttgaaaa gctaagcggt gcttattcca ctctgaaccg tgtaaagtat aaccttttgc
    11461 tctactgtac cctagctcca aatcattatc taacataatc tttcttaatg attgaatatc
    11521 agataaggtt gtattcttac ttactttcaa aaagtcttgg taatgactat tcgatataaa
    11581 atctaatcgg caaaaagtgt aaagatagat taaagctaag cgagtcgact ttggtaaaac
    11641 caattcatcc gacttaataa tatctgtcaa agactgcttc gtacgatttg ataaactata
    11701 gcgaccttgc tttttatcca gcactatccc tttattagct agataaggca ctaaataatc
    11761 tattccttct ttgacttcct ttataggtaa gctcacctta acagataatt catataatga
    11821 tagctcacaa tgatccatca aagtcatcaa aataactagt gctctataat caaacatttc
    11881 tcttcctttc taactacaat tatagtgtaa aaattataag aataaaagag tatttgagca
    11941 caatataatc tcacctttta aaagaaatta ttgggctgaa agtttttagt gattattttt
    12001 caactttcta ttgactttat aaattaaagt gatatcattt agatatcaaa taacaggagg
    12061 tcattcttat gactgaaaaa ctaatcaatt caaaaccaaa tggtgtattc gcattgattc
    12121 tcattgagtt gacaatcgta cttggtatct ttatatttat aatgggggtt ggttcggaaa
    12181 acatttttgg aattattatc ggacctttac taatcgtaat tgcaggtcta gctcatgctg
    12241 gtttaaaagt tgtcaaacct caagaagctc tggttctgac actctttggt aactatacag
    12301 gtaccatcaa agaacctggc ttttactttg tcaatccctt cagcgtagca gtcaaccctg
    12361 caaaccacac tcgacttgga caaagtggtg atgttagcac aaaatctcct tttttaggag
    12421 ctaaatcatc aaatgacaat gatgtaaatc ttgaaattgg caagaaacag atttccctca
    12481 aagtcatgac cttgagcaat tctcgtcaaa aaatcaatga ttgcttagga aaccctgtag
    12541 aaatcggtat cgcggtaact tggagagttg tcgataccgc taaggcagtc ttcaacgttg
    12601 ataactacaa agaatatctt tcattgcaat gtgatagcgc cctccgtaat attgtccgca
    12661 tctatcctta cgatgtgtct cctaatgtgg atactacggg tgatgggcaa gcagatgaag
    12721 gtagtctccg tggctctagc gaaattgttg ctaaccgtat tcgtgaagaa atccaaagtc
    12781 gtgttgagga tgctggcttg gaaatccttg aagcacgtat cacttaccta gcttatgctc
    12841 cagaaattgc tgccgttatg cttcaacgcc aacaagcatc tgccattatt gatgcacgga
    12901 agatgattgt agatggtgct gtaggaatgg ttgaaatggc actagaacgg ctcaatgaag
    12961 gggaattggt agaacttgac gaagaacgaa aagctgccat ggtttcaaat ctccttgtcg
    13021 ttctttgtgg caatcatgat gcacaaccaa ttgtcaacac aggaagcctt tactaaagat
    13081 ggctgaagct aaaaaaaagc agatccccct tcgactctca acaaagttat acgctgcact
    13141 cgcatcatgg gcagaagacg actttcgttc agtcaatggg caaatcgaat atctcctcac
    13201 agagtgtgtc aagcaacgga agaaagacgg aaaatacgta tcagaaacca ttgacgaacc
    13261 atttgagatt gatatttaac accttctcct gtctgccgtg tcggatagga gaattttttc
    13321 atcttttttt gtcaagtaac tttactttac aaaaaaaatg tgttatccta gtatggttga
    13381 tgaaaatcag tagattgaat cgaatataaa tacctaaagg agaaatcaaa atggcagtac
    13441 ctgcacgtcg cacttcaaaa gcgaagaaaa acaaacgtcg tacacactac aaagtaacag
    13501 ctccatctgt aaactttgac gaaactactg gagattactc acgttctcac cgtgtatcac
    13561 ttaaaggata ctacaaagga cgtaaaatca ctaaagctgc atcagctgaa taatagaagg
    13621 gagataccat gcgcgtaaat attacacttg aacacaaaga atctggtgaa cgcttgtacc
    13681 ttacttctaa aaacaaacgt aacactccag accgccttca attgaagaaa tactcaccaa
    13741 aacttcgcaa acacgttgtg tttacagagg ttaaataagc attcaatacg aatcaataca
    13801 gaagaaaaac gcttgatcag cgttttttct ttttgttaat ttcactacat tgtaagggaa
    13861 atcattcgaa tgactacatt tttcactacc ttttttgaaa taagaatacg gattcggaca
    13921 attaaaagca agaggaactc aaatgtaagt tggggttcct tttttgtata tttttcaaat
    13981 aacgcctcta cactatttgt agcaaattca ccaactacag ttgtatctta gttaaaataa
    14041 gttagaatat gtaagtgagt accagatata ccaagacatc gtcaccatct aaggtatatt
    14101 caaaatacaa aagttgacca actagatttc tgaatatcct tatatatcca ttcttaaaat
    14161 tggtttaaat agcgtagtct tttaaactag ttttgagaat ccaaaaaatc ttcctacata
    14221 tgtaagaaga ttttttagtt cagaatgatt agatttagct aatggatacc tatcctaccc
    14281 actcaccgtt agcattgact cgatagccat cgggtgtagt cgtgtttact aataaatctc
    14341 ctgaattata ggtatagtac catttaccag aaactgtaac ccaaccagta gccattgaac
    14401 ctgattcatt taagta
//
LOCUS       DAVBCB010000054        14254 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVBCB010000054 DAVBCB010000000
VERSION     DAVBCB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.054"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            3..350
                     /locus_tag="VQU28_001696"
     CDS             3..350
                     /locus_tag="VQU28_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477016.1"
                     /translation="MLRLPYKKQKISHFTVSEDKDICRYIRQQLYYQNLFWMKEQAEA
                     YQKGENILTYGLKEWYPQIRPIVGKFFQIEQDLTSYYQHFYTYYQKNPQNDWQKLYPP
                     AFYQQYFLKNMVE"
     gene            369..2171
                     /gene="pepF"
                     /locus_tag="VQU28_001697"
     CDS             369..2171
                     /gene="pepF"
                     /locus_tag="VQU28_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HET2477017.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEVLKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTFYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGIYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            2173..2886
                     /locus_tag="VQU28_001698"
     CDS             2173..2886
                     /locus_tag="VQU28_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HET2477018.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELENFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            2953..3894
                     /gene="prsA"
                     /locus_tag="VQU28_001699"
     CDS             2953..3894
                     /gene="prsA"
                     /locus_tag="VQU28_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HET2477019.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(4011..4352)
                     /locus_tag="VQU28_001700"
                     /pseudo
     CDS             complement(4011..4352)
                     /locus_tag="VQU28_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            4765..4860
                     /locus_tag="VQU28_001701"
                     /pseudo
     CDS             4765..4860
                     /locus_tag="VQU28_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5113..5733)
                     /locus_tag="VQU28_001702"
     CDS             complement(5113..5733)
                     /locus_tag="VQU28_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2477020.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLYTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(5733..6215)
                     /locus_tag="VQU28_001703"
     CDS             complement(5733..6215)
                     /locus_tag="VQU28_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HET2477021.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(6215..7057)
                     /locus_tag="VQU28_001704"
     CDS             complement(6215..7057)
                     /locus_tag="VQU28_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HET2477022.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDLKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(7152..7952)
                     /locus_tag="VQU28_001705"
     CDS             complement(7152..7952)
                     /locus_tag="VQU28_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HET2477023.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            8096..9475
                     /gene="glmU"
                     /locus_tag="VQU28_001706"
     CDS             8096..9475
                     /gene="glmU"
                     /locus_tag="VQU28_001706"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HET2477024.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIAPEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            9485..10030
                     /locus_tag="VQU28_001707"
     CDS             9485..10030
                     /locus_tag="VQU28_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2477025.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTARRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            10042..10356
                     /locus_tag="VQU28_001708"
     CDS             10042..10356
                     /locus_tag="VQU28_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HET2477026.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            10373..11065
                     /locus_tag="VQU28_001709"
     CDS             10373..11065
                     /locus_tag="VQU28_001709"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HET2477027.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNTQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            11142..11633
                     /gene="rocS"
                     /locus_tag="VQU28_001710"
     CDS             11142..11633
                     /gene="rocS"
                     /locus_tag="VQU28_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HET2477028.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            11731..12321
                     /locus_tag="VQU28_001711"
     CDS             11731..12321
                     /locus_tag="VQU28_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HET2477029.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGVLDLSQLFD"
     gene            12330..14093
                     /locus_tag="VQU28_001712"
     CDS             12330..14093
                     /locus_tag="VQU28_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HET2477030.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         4485 a         2552 c         2969 g         4248 t
ORIGIN      
        1 aaatattgcg tcttccctat aagaaacaaa aaatatctca ttttacagtt tctgaggaca
       61 aggacatctg tcgctatatc cggcaacaac tttattatca aaatctcttt tggatgaaag
      121 aacaagcaga agcctatcaa aagggagaaa atatcctgac ttatggactg aaagaatggt
      181 atccacaaat tcgaccaata gtgggcaaat ttttccagat tgaacaagac ttgactagct
      241 attatcagca cttttatacc tattaccaaa aaaatcctca aaatgattgg caaaagcttt
      301 atccaccagc cttttatcag caatatttct tgaaaaatat ggtagaatag aaaggatgga
      361 ggaatctaat ggtattacaa agaaatgaaa taaatgaaaa agatacatgg gatctatcaa
      421 cgatctaccc aactgaccag gcttgggaag aagtcttaaa agatttaaca gaacaattgg
      481 agacagtagc ccagtatgaa ggccatctct tggatagtgc ggataaccta ctagaaatca
      541 ctgaattttc tcttgaaatg gaacgccaga tggagaagct ttacgtttat gctcatatga
      601 agaatgacca ggatacacgt gaagctaagt atcaagagta ctatgccaag gccatgacat
      661 tctacagcca gttagaccaa gccttttcat tctatgagcc tgaatttatg gagattagcg
      721 aaaagcagta tgctgacttt ttagaagctc aaccaaagct gcaggtttat caacactatt
      781 ttgacaagct cttgcaaggc aaggatcacg ttctctcaca acgtgaagaa gaattattgg
      841 ctggagctgg agaaatcttt ggttcagcaa gtgaaacctt cgctatcttg gacaatgcgg
      901 atattgtgtt cccttatgtc ctagacgatg atggtaaaga agttcagcta tctcatggga
      961 tttacacacg tttgatggag tctaaaaaac gtgaggttcg ccgtggtgcc tatcaagctc
     1021 tttatgcgac ttacgaacaa ttccaacaca cctatgccaa aaccttgcaa accaatgtta
     1081 aggtgcaaaa ttaccgtgct aaagttcgta actacaagag tgctcgtcat gcagccctcg
     1141 cagcgaattt tgttccagaa agtgtttatg acaatttggt agcagcagtt cgcaagcatt
     1201 tgccactctt acatcgctat cttgagcttc gttcaaaaat cttggggatt tcagatctca
     1261 agatgtacga tgtctacaca ccgctttcat ctgttgaata cagttttacc taccaagaag
     1321 ccttgaaaaa agcagaagat gctttggcag tcttgggtga ggattacttg agccgtgtta
     1381 aacgtgcctt cagcgagcgt tggattgatg tttacgaaaa tcaaggcaag cgttcaggtg
     1441 cctactctgg tggttcttac gataccaatg cctttatgct tctcaactgg caggacaatc
     1501 tggacaatct ctttactctt gttcatgaaa caggtcacag tatgcattca agctatactc
     1561 gtgaaactca gccttatgtt tacggagatt actctatctt tttggctgag attgcctcaa
     1621 ctaccaatga aaatatcttg acggagaaat tattggaaga agtggaagac gacgcaacac
     1681 gctttgctat tctcaataac ttcctagatg gtttccgtgg aacagttttc cgccaaactc
     1741 aatttgctga gtttgaacac gccattcacc aagcagatca aaatggggag gtcttgacaa
     1801 gcgatttcct aaataaactc tacgcagact tgaaccaaga gtattatggt ttgagtaagg
     1861 aagacaatcc tgaaatccaa tacgagtggg ctcgcattcc acacttctac tataactact
     1921 atgtatatca atattcaact ggctttgcgg ccgcctcagc cttggctgaa aaaattgtcc
     1981 atggtagtca agaagaccgt gaccgctata tcgactacct caaggcaggt aagtcggact
     2041 atccacttaa tgtcatgaga aaagctggtg ttgatatgga gaaggaagac tacctcaacg
     2101 atgcctttgc agtctttgaa cgccgtttaa atgagtttga agcccttgtt gaaaaattag
     2161 gattggcata aaatggttga atcgtatagt aagaatgcta accataacat gcgtcgtcct
     2221 gtcgtcaaag aagaaattgt agacttgatg cgtcagcgtc aaaagcaggt cacaggtttc
     2281 ttgaaagaat tggaaaactt tgcccgcaag gaaaatattc ctattattcc ccatgaaacg
     2341 gttgcttatt tccgttttct tatggaaacc atgcagccta aaaatattct ggaaattggg
     2401 acggctatcg gtttttcagc tctcttgatg gctgaacatg cgccaaatgc taagattaca
     2461 actattgatc gtaatccaga aatgattggt tttgccaagg aaaattttgc ccagtttgac
     2521 agtcgcaagc aaatcactct cctagaggga gatgcggtgg atgtcttatc tacactgaca
     2581 gagtcttatg atttcgtctt tatggattct gccaagtcta aatacatcgt ctttctgcca
     2641 gaaatcctca aacatttgga agttggtggt gtggttgtct tggatgatat ttttcaaggt
     2701 ggtgatgttg ccaaggatat tatggaagtc cgtcgtggtc agcgaaccat ttatcgaggc
     2761 cttcaaaaat tatttgatgc aaccttagac aatccagaac tcaccgcaac attagtgcct
     2821 ttaggagatg gtattctcat gcttcgtaaa aatgtagcag atgttcaact gtctgaaagc
     2881 gaatgatttt cagaaaaatt taagaaaaaa tagtaaaata gatagagtaa cacttatctc
     2941 aaaggagtag acatgaagaa aaaattattg gcaggtgcca tcacactatt atcagtagca
     3001 actttagcag cttgttcgaa agggtcagaa ggggcagacc ttatcagcat gaaaggggat
     3061 gtcatcacag aacatcaatt ttatgagcaa gtgaaaaaca acccttcagc ccaacaagtc
     3121 ttgttaaata tgaccatcca aaaagttttt gaaaaacaat atggctcaga gcttgatgat
     3181 aaagaggttg atgatactat tgccgaagaa aaaaaacaat atggcgaaaa ctaccaacgt
     3241 gtcttgtcac aagcaggtat gactcttgaa acacgtaaag ctcaaattcg tacaagtaaa
     3301 ttagttgagt tggcagttaa gaaggtagca gaagctgaat tgacagatga agcctataag
     3361 aaagcctttg atgagtacac tccagatgta acggctcaaa tcatccgtct taataatgaa
     3421 gataaggcca aagaagttct cgaaaaagcc aaggcagaag gtgctgattt tgctcaatta
     3481 gccaaagata attcaactga tgaaaaaaca aaagaaaatg gtggagaaat tacctttgat
     3541 tctgcttcaa cagaagtacc tgagcaagtc aaaaaagccg ctttcgcttt agatgtggat
     3601 ggtgtttctg atgtgattac agcaactggc acacaagcct acagtagcca atattacatt
     3661 gtaaaactca ctaagaaaac agaaaaatca tctaatattg atgactacaa agaaaaatta
     3721 aaaactgtta tcttgactca aaaacaaaat gattcaacat ttgttcaaag cattatcgga
     3781 aaagaattgc aagcagccaa tatcaaggtt aaggaccaag ccttccaaaa tatctttacc
     3841 caatatatcg gtggtggaga ttcaagctca agcagtagta catcaaacga atagtccaaa
     3901 tcaatgagtc agggaaaaaa actcgacttc aggaaaaaat gaagcaaaca ttcccacaat
     3961 aaaacgcata gtacaaggtt tgtactgacc ccaaaagtta gacaattaat ttatctgaag
     4021 gatttagttc tgtattgcac agggctaagt ccttttagtt ttaccttaat tctcttattg
     4081 ttgtaataat caatatagtc tataatggct tgttccaatt gattaagtga tttaaatgtt
     4141 ttctcatagc cataaaacat ttcggatttt aaaatgccaa agaaagattc catcctaccg
     4201 ttgtcttggc tgtttccttt gcgtgacata gatgcttgaa ttcccttact ctctaggaag
     4261 cgatgataag aatcgtgttg gtattgccag ccttggtcac tatggagaat cgtattctcg
     4321 tagtgcttct ctttgaatgc ctgttccaac attaacgatc aatcaattta atcatgtacc
     4381 taatattaga attgtttatc ccaaatttat ttgaaagctt ctctaagcta tatccttgtt
     4441 ttctaagttc atagatctga actttatcat cataagttaa tttcataata aaaacacccc
     4501 aaaagttaga ttttttctgt ctaacttttg gggtgtagtt catgtacacc tgatatgatg
     4561 cgttttataa ttttaaagac tttttgacca gcctcatttt tttaacttga tactcagtga
     4621 aaagcaaaga ttaaactagg aagctagctg taggctgctc aaagaacagc tttgaggttg
     4681 cagataaaac ttgtgaggtc accaacatat ataatgtgaa gctgacgtga cttgaataga
     4741 ttttagaagg gtatgagtct ggaagtttta atggataatg caagattcca tagaatgggt
     4801 aagctagagt tcttatgtga agagtttggg cataaacttt taccttttcc tccctactca
     4861 tcttagtata gaaaagtgaa tctaaaatag tacataactg cttctaaaac attcttataa
     4921 attgatttaa attctcaaat catattattc agttcttatt tcattttgct ctacaatcct
     4981 gttgagaaga cacgtgttca tatcaaaaag gtattggcaa gttgcaatac ctttttacaa
     5041 ggttcttttg tcttattttt gtttcaactg actatatctc ctatggttct agttcagaag
     5101 gctaggctat aattatgatt gataagaagt atcattccaa gtattgagag tgaatgtttc
     5161 aaaatcatgg gtttctataa tggtcaggct ggcatttgct agaccgccat ctttacgaag
     5221 aagtggttct ttatagccta ggagagtacg aagactggca gtaagattgg cgccgtgtcc
     5281 gacaattaga atacgctcag ctggactatc ttttaatgat ttgataaatt ggatggtccg
     5341 ttgagttgta ctatagaggg attcggctcc gaacattcga gtgtcaaatt gagcaagatt
     5401 tgaacgaaaa gcctggattt gttgcgggta aatagcttcc aaggttgcaa ttttcaaacc
     5461 ttctaacttc ccaagttgcc attcacggag attaggaacg atttctaaag aacagggggt
     5521 atagagttga ctttggataa tctcagcaga tttgaccgct cgaggtaaat cacttgaata
     5581 aatctgatca aaaggaattt ccttgagata ctggccaagt cgttttaggg tttcaatgga
     5641 ttcaggaaga agaggagaat caccgctagc accttgaaaa cgaccttctt ggttccagag
     5701 ggtacgaccg tggcggacaa agtagagttt cattacttga tgtcctccaa aatatctaca
     5761 aagtctgcct ttacaaagct agccaagtct tgtggcgcga cgataatgct gtgtccgact
     5821 tcgcctgcag agacaatcat tcgatccaaa tctagagcaa ttttatcgat aaaaatggga
     5881 taattgtgtt tctgacgaat tccgacagga ttattggctc catgaatgta accagttgtt
     5941 ttttctaagt ccttttgtgg aatcatgctc acttttttat tgccagaaat tttagctagt
     6001 ttcttttcag acaagtgctg agtgataggg acaattccga taatcggtcc ggtcttgtct
     6061 cccaaaagcg ccaaggtttt gaaaatctga tctcgttcat aaccttgagg aagctctcct
     6121 tctagggcat tgatttgaat cccctgatga gggatagctg ctttagatag gatttgttcc
     6181 accaatgttt ttttgatttt aacttttttt gccattattt atatttatcc tccaattgac
     6241 tcatccaaat accaagccag attcccagcg caaagaagaa ggcgatgatg acataaccga
     6301 caagtgaaag tcctgtgtat tggatacttt cagcgtttcc tgcatttgga attaagatca
     6361 aaagggtact tgataggacg ataccgatga tgaaatgata gacgcgtgag tggtagttgt
     6421 ttaaggcata atccatcaat tttgaaaaaa cgatgagagt tgcacctgca ccaattccaa
     6481 tcggaaagaa ggttcccaag agatcaaaag ttttaaaacc agtcaacata ggagcataga
     6541 gtcccaaaat caaaagtaag ttcgatgggc tgaggccagg aaccaagacg ccaagggcca
     6601 atagtgcgcc tgctaggacg aagttaagaa agctggcgct taaggttcca acgacaaaat
     6661 ttaaggcata gagtcctaat ccagaaatga taaaggttgt ccataaccaa gctaaatcaa
     6721 tcttgtctcg gtcagattct cgagttgatt ctttgaggag gctaggaact gtaccgataa
     6781 tagctcccgc aaagctccat aatacaaaga cctgataatt ttcaagcagg tattcaatcg
     6841 ggtaggaaaa taagccgatt cccagaagca taccgatggc aactggaata aagtacaaaa
     6901 cattttcttt aaggtcttta aagggatggg ccagaaagcc aatcattcgt tcatagattc
     6961 ctaagattgc tgctagaacc cctccggaaa ttcccggtag gataaatcca agagcaatta
     7021 caatcccttt aataacgcgt gctaaccatg agagcatata tttcctccaa ctatttctat
     7081 ttcaaaaatt ccttactata ttgtatcaca atcagacaca aaaagaaaaa gcaaatgata
     7141 aacaaatgct tttaaggttt taaaaaaagt tttcgaaagg tttcttcttt attttttaag
     7201 ggagagataa cgttgatatc taaatcgtgg tcaaagccgg caatttttcc tttagatgtg
     7261 tattggtgaa tatcataatc taaatcagtt ttaggactgc tctccaaaaa tcctgagtct
     7321 gagccgtagg acggaatcca aacagaggta aacttgcctg tatcaatact gtgttcttcc
     7381 atgaagtaga caccaacgta gatgccgatg tttttagcac ctagtgatgc tagttttgct
     7441 cgaaagtttt cgacaccttc gttcatatta gacatggttt tgtcttccac gtcaagccaa
     7501 tagtaactag ggctgtaagg agaggcagca ttgtagaaaa cttcggcagc cttttccatt
     7561 tcttggacac tttttccagc tacataagcg tagacagcaa ctgggacatt ccgcttttga
     7621 agttcagtga tatgactctt ataggccttg tctattccat tgataaatga agcatcattt
     7681 tcttttgttg tttgagcacc actgtgaaca cgaacaatag cacctgaaat attttgtgag
     7741 agggcatcgt agttgatttc ctcaggacgc tgccagccag agaggtcaat aatcggtttg
     7801 tctaagtgtt tcaaagcctg tgcttcaatc tgtgctatat tggattttgt tttaaacgat
     7861 tggctgtcat taagtgggcg attgatgatt aaaatgaaca tcataatccc aaaaaaacta
     7921 aataaaataa gtggatgaat ttgttttctc atatcttata agtctaccct aaaaatcaaa
     7981 aaaaatcaaa aaaatgggtt aaggaagaga ctttagagca ttttttcatt caagagtgcg
     8041 gaatgatttg aaatatggta taataaaagg gaatttctac agaaaagaga agattatgtc
     8101 aaattttgcc attattttag cagcgggtaa agggactcgc atgaaatctg atttgccaaa
     8161 agttttgcac aaggttgcgg gtatttctat gttggaacat gttttccgta gtgtgggagc
     8221 tatccaacct gaaaagacag taacagttgt aggacacaag gcagaattgg ttgaggaggt
     8281 cttggctgga cagacagaat ttgtgactca atctgaacag ttgggaactg gtcatgcagt
     8341 tatgatgaca gaacctatct tagaaggttt gtcaggacac accttggtca ttgcaggaga
     8401 tactccttta atcactggtg aaagcttgaa aaacttgatt gatttccata tcaatcataa
     8461 aaatgtggcc actatcttga ctgctgaaac ggataatcct tttggctatg gacgaattgt
     8521 tcgtaatgac aatgctgagg ttcttcgtat tgttgagcag aaggatgcta cagattttga
     8581 aaagcaaatc aaggaaatca acactggaac atacgtcttt gacaacgagc gtttgtttga
     8641 ggctttgaaa aatatcaata ccaataacgc tcaaggcgaa tactatatta cagacgtcat
     8701 tggtattttc cgtgaaactg gtgaaaaagt tggcgcttat actttgaaag attttgatga
     8761 aagtcttggg gtaaatgacc gtgtggcgct tgcgacagct gagtcagtta tgcgtcgtcg
     8821 catcaatcat aaacacatgg tcaacggtgt tagctttgtc aatccagaag caacttatat
     8881 cgatattgat gttgagattg ctccggaagt tcaaatcgaa gccaatgtta ccttgaaagg
     8941 gcaaacgaaa attggtgctg agactgtttt gacaaacggt acttatgtag tggacagcac
     9001 tatcggagca ggagcggtca ttaccaattc tatgattgag gaaagtagtg ttgcagacgg
     9061 tgtgacagtc ggtccttatg ctcacattcg tccaaattca agtctgggtg cccaagttca
     9121 tattggtaac tttgttgagg tgaaaggatc ttcaatcggt gagaatacca aggctggtca
     9181 tttgacttat atcggaaact gtgaagtggg aagcaacgtt aatttcggtg ctggaactat
     9241 tacagtcaac tatgacggca aaaacaaata caagacagtc attggagaca atgtctttgt
     9301 tggttcaaat tcaaccatta ttgcaccagt agaacttggt gacaattccc tcgttggtgc
     9361 tggttcaact attactaaag acgtgccagc agatgctatt gctattggtc gcggtcgtca
     9421 gatcaataaa gacgaatatg caacacgtct tcctcatcat cctaagaacc agtaggagcc
     9481 tatcatggag tttgaagaaa aaacgcttag ccgaaaagaa atctatcaag gaccaatatt
     9541 taaactggtc caagatcagg ttgaattacc agaaggcaag ggaactgccc gacgggattt
     9601 gattttccac aatggggctg tctgtgtttt agcagtaacg gatgaacaaa aacttatctt
     9661 ggtcaagcag taccgcaaag ctatcgaggc tgtctcttac gaaattccag ccggaaaatt
     9721 ggaagtagga gaaaacacag cccctgtggc agctgccctt cgtgaattag aggaagaaac
     9781 agcctataca gggaaattag aactcttgta cgatttttat tcagctattg gcttttgtaa
     9841 tgagaagtta aaactatatt tagcaagcga tttgacaaaa gtggaaaatc cgcgtccgca
     9901 ggatgaggat gaaaccttgg aagtccttga agtgagctta gaagaagcga aagaattaat
     9961 ccaatcaggt catatctgtg atgccaagac aattatggct gttcagtatt gggagttgca
    10021 gaaaaaatag aggaggtcag tatgggtaaa tctttattaa cggatgaaat gattgaaaga
    10081 gctaatagag gcgaaaaaat ttcaggtcct cctttgctag atgataatga ggaaactaag
    10141 attttaccaa cctcttcttc ccgttttggt tatgccaatc ctaaggatca tggttttagc
    10201 caggaaacct tgaagattca ggtcgaacca tctattcata aaagccgtcg tattgaaaat
    10261 accaagagaa atgtcttcaa ttctaagttg aataaaatct tatttgcggt catctttctc
    10321 ttgattttgc ttgttttagc aatgaaactt ttgtaataga aaaggaattg aaatgaaaat
    10381 aggaattatt gctgctatgc cagaagaact ggcttatctg gtccagcatt tagataatac
    10441 ccaggagcaa gttgttttgg ggaataccta tcatacagga accattgctt ctcatgaagt
    10501 cgttcttgta gaaagtggaa ttggtaaggt catgtctgct atgagtgtgg cgattttggc
    10561 tgatcatttc caggtggatg cccttattaa tacgggttca gctggggcag tagcagaagg
    10621 tatcgctgtt ggggatgtcg tgattgctga caaattagcc tatcatgacg tggatgtcac
    10681 agcttttggc tatgcttatg gacaaatggc gcaacaaccg ctttatttcg aatcagacaa
    10741 aacctttgtt gctcaaatcc aagagagttt atctcaattg gaccaaaact ggcatcttgg
    10801 tttgattgct acaggagata gttttgttgc aggaaatgac aagatagaag cgattaagtc
    10861 ccatttccca gaagttttag ccgtggagat ggagggggca gctattgctc aagcagcgca
    10921 tgccctcaat ctcccagtct tagtcatcag agctatgagt gacaatgcca accatgaagc
    10981 aaacatcttt tttgatgagt ttattatcga agctggacgt cgctctgccc aagtcttgtt
    11041 gacctttttg aaggctttag attaagcgga aatttgacag tttttctagc ttatgataag
    11101 atttaagtaa agaaaagcta gaaaacgttt cagaggatat tatgagtatt gaaatgaccg
    11161 tcagtgagat tgcagaggtc ttaggattat ctcgccaagc aatcaataac cgtgtcaaag
    11221 aattaccaga agaagacaca gataaaaatg acaaaggggt aacagttgtt accagaagtg
    11281 gcttgattaa gctagaagaa atctataaaa aaacgatttt tgaagatgag cctgtcagtg
    11341 aagatgtcaa acaacgtgaa ctgatggaga ttctagtgga tgagaagaat gcagaaattc
    11401 ttcgtcttta tgaacaatta aaagccaagg atcgtcagtt atcagaaaaa gacgagcaga
    11461 tgcgtatcaa agaccgtcag attgctgaga aagacaaaca attagatcag caacaacaat
    11521 tgacccttca agccatgaag gatcaagaaa atcttaagct agagctggac caagcaaaag
    11581 aagaagtcca atccactaag aaaggctttt ttgctcgttt atttggagga taatcaagga
    11641 gctgtttagg ttaaatgcta acctgatggc ttcttttgtt tgtgaatagt atagttgatt
    11701 agaacagaga cagtagagga gaggtacaga atggaacgat tagaagatta cattgctttt
    11761 gacttagaat ttaatcagca cgaaggacaa acacacttga ttcaagtatc ggcagtgcgt
    11821 tttagagatg gtcgggaaat cgatgcctat gattcctatg ttcataccag cgtccctcta
    11881 aaaagcttta tcaatggttt gaccgggatt acagctgaga ccttaaaaga tgctcctcca
    11941 gttgagaaag tcttagaaga attccaagag tttgtgggtt ctttgctgat gataggctac
    12001 aatgctgcta aaagtgattt acctatttta gcagagcatg gtttagacta tagccaacag
    12061 tacaaggtgg atttgtatga tgaagctttt gaacgtcgga gttcagattt acatggtatt
    12121 gccaatctca aattgcaaac tgttgcctca tttcttggat ttcaaggtca gtctcataat
    12181 agtttagagg atgctcggat gacggcgcgt gtttacgaat ccttcttaga gtcggatcaa
    12241 gctagggaat tgttagagac agaaagtagc ttatcaaaca atccttttgg tgtcttggac
    12301 ttgtctcaat tatttgacta ggagtgccta tgaaacctga aaaacctaaa aaattgggtc
    12361 ttaaacagat ttacctcaat cttgataaaa tcctctttct atttttcttg atttttatga
    12421 tagtggagta tttgtgggta ccattcaatt catggttggc tagcttctta ttagatcaga
    12481 caggttatct ttttatttcc tataacaatg tcttagccat ccttactcat tctccattga
    12541 ttagtctagc ttttttagcc ttgattgtaa ttaaccttct ggttgcttat tttcaaatag
    12601 gactcctctt tattggtgct cgtcatctcc tctataatga aaagcgaagg ctaattgagt
    12661 atatccgcaa ggttttccat gaaagttttg gatttatgaa aaagctaact tgcgctaaag
    12721 ccttgtttat ctttttctat atagctatgc tttttccttt tatacggaaa atgctgaaga
    12781 tttattatct taataaaatc gtcattccag agtttatcca agcttattta gaagataaat
    12841 attggatgtg gtggctgagc atcctacttc tatctcttat ctttctctat gtctctgtca
    12901 gattgatgtt tgcccttccg aagattgtat atgatcagct gactgttcga gaagcagtga
    12961 tgtttagctt ggagaaaacc aagaagagag ttacttttta tgcttggaat ttattttaca
    13021 tcttgctcaa agcacattta ttattttatc ttcctttgat tcctttatta ttggctcaaa
    13081 ctttggtaga tgatatcact cagagagagt ctctgattct tggtattttg aattttgtcg
    13141 tgattaaaaa tctctattat atggctctga cttatttttt ggttaaattt gtttcatttt
    13201 tgacaggaaa ggagctggat atgcttccta ggagagaaaa agatcacatc gtgcgatggg
    13261 gtgtcatgac ttgtgccagt cttttctttg ccttagaagg ttatatttat ctggaggctc
    13321 ccatggttca tctacctcaa cttatttctc accgaggggt ttccaatgca aatgggattc
    13381 aaaatacagt agagtccttg gaaactacag cacaactcaa accagacttg gtggagacgg
    13441 acgtgcagga aacaaaagat gggcagtttg tcatgatgca tgatgctaac ttgaaaaatc
    13501 tagcaggtat caataaaagt cctcaagact taaacttgga ggagcttaaa gggattgata
    13561 tttttgaaaa tggctaccag actaaaattt caagctttga agattatctc agtcgagcca
    13621 acgaacttgg tcaaaaatta ctaattgaaa ttaaaaccag taaaaaagat agtccagaca
    13681 tgatgaaccg ctttttagcc cgttatgctg caaagctcaa gatttatgga catcaaatcc
    13741 agtctttaga ctaccatgtt gtcgaaaaag taagacagta tgatgccgaa ctgccagttt
    13801 atttcatcat gccctacaat tctgtctttc ctaaaacaag agcgacagga tatactatgg
    13861 agtactcaac cttggatgaa tattttgtaa gcaaactatg gacaacggat cagaaacttt
    13921 atgtctggac tgtcaatgat tcagaagcta tcagcaaatc tcttcactta ggagtagatg
    13981 gagtgattac agatgatttg gaaaaagttc agcgggaaat agaagtagcc caagaagatc
    14041 cagaatatac ggatttgctc ttgaaaaaag cattggaatt ctttgaattt tagtaataaa
    14101 aaagaggaac ggttgttcct cccatgatag gtaagtattc ttgtgaatgc ttgctttttt
    14161 gtggttgcaa tccgtttacg agaattgttt gtattgttct tgtttcaatt tactttatag
    14221 tgctttgaag ttggaatgct acactataac ttct
//
LOCUS       DAVBCB010000055        13012 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVBCB010000055 DAVBCB010000000
VERSION     DAVBCB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.055"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            338..1689
                     /locus_tag="VQU28_001713"
     CDS             join(338..779,779..1689)
                     /locus_tag="VQU28_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2477031.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNGMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
     gene            complement(1924..2532)
                     /locus_tag="VQU28_001714"
     CDS             complement(1924..2532)
                     /locus_tag="VQU28_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HET2477032.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            2698..2868
                     /locus_tag="VQU28_001715"
                     /pseudo
     CDS             2698..2868
                     /locus_tag="VQU28_001715"
                     /inference="COORDINATES: protein motif:HMM:NF013577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
     gene            2857..3468
                     /locus_tag="VQU28_001716"
     CDS             2857..3468
                     /locus_tag="VQU28_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001821036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HET2477033.1"
                     /translation="MTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIEL
                     AIKLIREANQILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDL
                     IIAFSLSGSTKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGG
                     SLMAKASQLFIIDVICTRLSLINYEDTICKKEEIASLLSNKVE"
     gene            4185..4871
                     /locus_tag="VQU28_001717"
     CDS             4185..4871
                     /locus_tag="VQU28_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HET2477034.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMAAISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            4872..5804
                     /locus_tag="VQU28_001718"
     CDS             4872..5804
                     /locus_tag="VQU28_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2477035.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFFFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            5815..6744
                     /locus_tag="VQU28_001719"
     CDS             5815..6744
                     /locus_tag="VQU28_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2477036.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLRNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            6758..8197
                     /locus_tag="VQU28_001720"
     CDS             6758..8197
                     /locus_tag="VQU28_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477037.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            8266..9843
                     /locus_tag="VQU28_001721"
     CDS             8266..9843
                     /locus_tag="VQU28_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2477038.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKSFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            9903..11363
                     /locus_tag="VQU28_001722"
     CDS             9903..11363
                     /locus_tag="VQU28_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HET2477039.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKTIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            11385..11840
                     /locus_tag="VQU28_001723"
     CDS             11385..11840
                     /locus_tag="VQU28_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HET2477040.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            11927..12332
                     /gene="cdd"
                     /locus_tag="VQU28_001724"
                     /pseudo
     CDS             11927..12332
                     /gene="cdd"
                     /locus_tag="VQU28_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221146.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
     gene            12345..12794
                     /locus_tag="VQU28_001725"
     CDS             12345..12794
                     /locus_tag="VQU28_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HET2477041.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDILKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEETILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHRTGLLVEEPLNIKKVVFKVKI"
BASE COUNT         4617 a         1682 c         2416 g         4297 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagggatt ctctgaagtt atggatgtcg ttccaatatg tgtacgttgg aatgttagtg
      121 cttatatggg gggaacagaa tcctctcttg attgaagaca agttagccat taggctggtt
      181 tgtctctttg tcaactgtag tgggttgata taatagtatt agtgagtggg ataaaagttt
      241 catttagttt attcagtaca aatttaacgg gtcaagattt attgaactgc accccaatag
      301 ttagacagaa aaaatctaac ttttggggtg ttttattatg aaattgagtt atgaagataa
      361 agttcagatc tatgaactaa gaaagcaagg acaaagcttc aaacagcttt caaaaagatt
      421 tggtgtggat gtttctggtc taaagtacat ggtgaaatta attgaccgtt atggcataga
      481 aatcgttaaa aaagggaaga atcgtcacta ttcttctaaa ttgaaacaag aaatgatgga
      541 taaagcctta ctggaaggtt gttcacaaag aagtattagt cttgattatg ccctcccaaa
      601 ccaaggaatg ctttcatttt ggccggcaca atacaagaaa aacgggtata ctattgttga
      661 gaaaacaaga gggagaccag ctaaaatggg acgtaaacgg aagaagactt gggaagaaat
      721 gacagaacta gaacgactcc aggaggagaa tgaacgctta cggactgagg tggcctacct
      781 aaaaaagtta aaagagttag aggaaaggga cgaagcccta gagcgagaaa ggcagagaca
      841 gttagagaaa tggtttcagg aggatttcga ctagattttc ttcttgaaac agcacgttta
      901 gctcgctcga cttactatta tcagttgaaa caactggatg gggttgataa agataaagag
      961 attaaaaccg aaattcaggg cattgataat gaacataaag gcaactatgg ctatcgtcga
     1021 atccatttag aactaaggaa tcgtggtttt gtggtcaatc ataagaaagt tcaacggttg
     1081 atgagaattc ttggtttaac ggctcgaatt cgccgtaaac ggaagtattc ttcctaccaa
     1141 ggagagattg gcaagaaagc agagaatctc attcaacgcc agttcgaagc atcgaggcca
     1201 atggaaaagt gctatacaga tgtgacagag tttgccattc caaatagcac acagaaactc
     1261 tatttatcac cagttttaga tggttttaac agtgaaatca ttgcttatca tctttctact
     1321 tcgccaaact tagaacaagt gaagagtatg ttagaacagg cctttacaga gaaatactat
     1381 gagaatacga ttctccatag tgaccaaggc tggcaatacc aacacgattc ttatcatcgg
     1441 ttcctagaga gtaagggaat tcaagcatcc atgtcacgca agggcaacag cccagacaac
     1501 ggcatgatgg aatctttctt tggcatttta aaatccgaaa tgttttatgg ctatgagaag
     1561 aactttagat ctttagaaaa ccttgaacaa gctattgtgg actacattga ttactacaac
     1621 aacaaacgaa ttaaggtaaa actaaaagga cttagttctg tgcagtacag aactaaatcc
     1681 tttggataaa ttatttgtct aattttttgg ggtcagtaca gtattaaata ccatgatctt
     1741 atgcgtttta taataaaaaa tctgttcttg ttttattttt agaaaattta ctaaagacta
     1801 aaaatattat gaataattta aaaaatagtc aggttatata gttttttgaa attaaaatta
     1861 ttttatcgtt gaaattagtt aaaaattttt ataaaatcaa gatgcaaggc tatgagatgt
     1921 tctctagttt aatttttcat acttcgagaa tataaagtta tataaaaatg cattagcata
     1981 ttttgttatt gagggaagta taaaaatcat taaagttatc caaactagta attgattctg
     2041 taatataaag aagagtagaa ctgggataat ttcaataatt ctaataagaa cggcaataaa
     2101 aaaatgttta taggctaaca tgattgaatt gtgtagagaa ttttttaaag tgttattata
     2161 tctaccgatt aaaggaatta gaaatgttgt tgctattaaa tgaagtatga tatatattac
     2221 tccaatatgt cgtaagatag aaatgatata tataggtaat ggtatgtagg gtaggaagaa
     2281 caaactgaat aataagatta aaattggtaa attagatatt aatgctgctc ctaaattttt
     2341 ccaataatct ttaaaaaaac tgtttgcgaa gtctctatag ggataggatt cctcgtgtcg
     2401 tacaattttt attagagttg ctattagggc actttcacct gatccgatag caataactgt
     2461 tactactgac gctacataga gtagatttaa cattataaga tttgaaactt tatcaataaa
     2521 ttttatctgc atctttaact cctgctgttt ttatacttat aatatcacat catttgagtt
     2581 tcatcaactt tgaatacatt aagaaagaat ataactatga ggaaaggaaa aaattattat
     2641 gaataaatcc aaaaaatata aagaaagaat tcattatgaa tacgataatc ttactaaatc
     2701 agagaaaaaa ttgctgacta tattttagtt agagatagtg aaatagttta tgaaacgtct
     2761 tcagaaattg ttagtaaagt aaaagttgga gagggaactc ttaatcgatt ttgcaaaaag
     2821 attggatact ctggttttca ggaactaaaa ttaaaaatga cgaaagatat attagagtta
     2881 gaatctcaaa aaatgagtag tgacacattt attgatgaaa taaaaaataa ttatttatct
     2941 attgtggaaa gtacaagaaa attaattgat ggtagacaaa tagaattagc tataaaatta
     3001 ataagagaag caaaccagat tttaatgatt ggtgtgggtt ctagtggaaa tgcggctaga
     3061 gaatttgaga gtagtttgtt aagaattgga ataataagta agacggtaat tgatacccat
     3121 tttcaactca tgcatactgc gttattgaaa gataatgatt tgataatagc gtttagttta
     3181 tctggttcca caaaagaagt tgaggaaact ttattaaacg caaaaagaaa aaatgttaaa
     3241 attatttcaa taacaaatta ttcgtccaga aatatagcaa aattatctga ctgcgttctt
     3301 ttgacatcaa aaaaagaatc ttatttagag ggtggtagtt taatggctaa ggcttctcaa
     3361 ctattcataa ttgatgtaat atgcactaga ctctctttaa taaattatga agatactata
     3421 tgtaaaaaag aagagattgc ttctttgctg tcaaataaag tggaataaat caataatttt
     3481 taaattttaa taaaaatttt ttaaaataaa ttaaaaaacg atcctaaaag ggtcgttttt
     3541 ttgctaaagt tttaaaaata aacaataatt aagattatag tgcgttgaat ctatattagg
     3601 acataacaat tcttaaatca ttgttagaaa ttagtttgaa ttttccaatc aatttgttcg
     3661 atttttacat cattgcatta tatcacgtga ctattaacct tgttttctcc taaaattgat
     3721 tttcttcttt tcttatcttg tccactcgaa acaagtattg taagaatttg attatttttg
     3781 aaagtacttt taatatattt gagatagtta aaaaagattt gaaactaaat tccaaattag
     3841 aaaaagactt gaaatgctaa aaaaaaaaag tatactctaa ttgaaaacgg taacaaaact
     3901 aatttagaga atgaaatata gagtatttct ctcttaaaag tttttggtga aacgagatgt
     3961 agaaaggaga tttagccaaa gagtctatta gtgctagaat aatagattag aattatagtg
     4021 tattgaatct ataacagtac gccttaaatg ctaaaatatt tctataaatt aatttaactt
     4081 tcctgatagg gctgttcata tcttatttca attcactata taaataaaat tgtagtagat
     4141 gttattgaat tagtaaaaaa aataaaaaaa cggaggtttt ctgaatggaa actaaaaaaa
     4201 ttaaaaattt aaaaggtcaa ataattgttt cctgccaagc gttagaagga gagccacttt
     4261 atactccaaa tggaggagtg atgccgttat tagcaaaagc cgcctttcaa gcaggcgcta
     4321 aaggtattcg tgctaattct gtccgcgata tttcagaaat aaaagaagaa gttgacttac
     4381 caattatagg tatcataaaa agagattacg atggctttga accttttatt tcagcaacta
     4441 tgaaagagat tgatgaatta gttagtgaag gagtagacat tttagctttg gattgtacta
     4501 atcgttcaag accaggttat gataatatta ctgactttat tcatgatatt aaagtcaagt
     4561 atccaaacca attattaatg gctgctattt ctacttttga agaagggaaa gtagctgctg
     4621 aatcgggagt agattttgtg ggtacgacat tatctggtta tacaccatat agccctaaaa
     4681 aagataatcc tgattttgag ttagttgaaa gattggtaaa agagcttgac gtaccagtta
     4741 ttgctgaagg aagaataagt actccggaac aagctagaaa aatgttagat ttaggtgctt
     4801 acgcagtggt tgttggagga gctatcactc gtcctttgga aatagcgaag aagtttattg
     4861 aggtagttta gatgaatcaa gaaatattaa aaaagagtaa gattcaattg acagttgaac
     4921 atattcaaaa atattggatt ttatatttaa tgatgattcc agcaactgtt ctactaattt
     4981 tatttacgta tggaccaatg tacggcatta ttatggcctt tcaagacttt actgtcttca
     5041 agggatatac tggaagtcca tttgtcggtt taaaacattt tcaaagattg ttctcagatc
     5101 ctttgtttta tagattgttc aagaatacgt tcatggtagg ggttttagat tttttattct
     5161 ttttcccagc tccgcttatt tttgcactca tattaaacga agtcagaaaa gtaagattta
     5221 agagtgtagt tcaatctatt agttatcttc ctcattttat tcccctagtg gttatggttg
     5281 gtattatttt tgaactattt ggttcatatg gtattattaa cagtttactt tcatcacttg
     5341 gaatggagcc aataagtttc tttacaaaat ctgaatggtt cttgcctctc tacattggtt
     5401 caggagtatg gaaaacaata ggttggggtt ctatcattta tatgggagca ttaaccaata
     5461 tagattcaac tttatatgaa gcagcagata tggacggggc taatagatgg cacaaaatgt
     5521 ggcatgtaac tctaccaagt ttaagaccta cagtagttac tttgtttata ctaaatgctg
     5581 gtggtatcat gcaagtagga tttgaaaagg tattcttgat gtctagtcct gcaacatatg
     5641 aagtatctga tgtattatct acttatgttt atagacaagg tattttaaat tctgatttca
     5701 gttactcagc tgcagtagga ttatttaata acatagtagc tttgttattt gttttattag
     5761 ctaataagat tgctaaaaaa cttggcgaag aaggaattgt ataagggggg taaaatgatt
     5821 ttttcaaaaa atacaaaaaa gagtaataga gacaaagaaa ccttcgttga ttatctcttt
     5881 atattcattt taataataat aaccttactt atgttgtatc cattatggtg ggtagtgatg
     5941 acgtcacttt cagaaccagt ttggctatct aaaaacgttg ttaaattatt tcccttaaaa
     6001 atagatatta ctgcatataa gtttattttt ggggattcta aactatggca agcatatggt
     6061 aatagtgtat tgtatgctgc actatctaca attgttagtg ttgcggtatg tagtatgtat
     6121 gcctatccac ttacaattcc tgaaattagg ggtaaaaaat tctggaatac tcttattttg
     6181 attccaatgt tctttagtgg aggaatgatc ccgctttata tacaagttgc aaacctcggt
     6241 ttattgaata ctgcttgggc tcttgtttta cctggtgctg tttctacata ttatataatt
     6301 ttgtttagaa cacagtttaa atcaatccca atggagcttc gtgagtctgc tagaattgat
     6361 ggggcaagtg aatttagaat tttttggaat ttagtattac cattatcaaa agttattttc
     6421 atgattatag cactttattg catagttggt tcatggaata gttttactcc tccattactt
     6481 tatttgaatg acgacactaa gatgccttta tctatttatt tgagaaattt agtcgttaca
     6541 aatacaactg atactgctga attatcactt tctgcaggta atacatatta ccaacaaatt
     6601 gctgctcaag gtggaggtat tggtatgcgt attgctatta agatgggtac tattgttgtt
     6661 agtgttattc caattatgat gatttatcca tttattcaga aacattttgt taagggaatc
     6721 ctgctaggtt ctgtgaaagg ttaataaggt aattattttg caaaatttaa aaaaatatct
     6781 tttattagat agcggtttag tagttgggta tacattagaa aatgctcaat taaaattaaa
     6841 taagctaaaa aaagatactg aaaacaatcc actatttggt gaagagtatt ttgccgaaaa
     6901 tcctaaatta tgggaagttc gttttgataa ttcatatcca aacgttatct atgataaaga
     6961 attgaaatta tatagatgtt ggtatcaaac atttgttagt gatgaagcaa gcgaagagac
     7021 tcctttagca gaacgtggag agaaggaata cattgtcaag tcatctagaa tgacagcact
     7081 ttgctatgct gaaagtaaag atggagtaaa atgggaaaaa ccaaatctca atctagtaaa
     7141 atttaaaggt tcaaaagata ataatattgt tctgatgcat gcacacggtg caggtatcat
     7201 gctagatgaa caggagttgg atcctaagaa acgatataaa ttagtttcaa aattagaatt
     7261 ttctccgaat cttcattaca tggttgttgt tttttcagaa gacggtattc attttgacga
     7321 acctattcca tggaaaaaat ggaatcctgc agcagatagt cataattttc catttagaga
     7381 tccaaaaact ggtaaatacg ctgttattac aagaacctgg gcaaataacg tacgtctaag
     7441 tgcaattact ttcagtgatg attttattaa ttggtcagaa cctaaagaaa tccttagggg
     7501 agatggattt gaagatcaaa tttattctat gccagtaatt tcatacgcgg atatctattt
     7561 aggttttgct tcaatgtacc atgaaggcga cactttaaat gaaaactatg ataaagtaga
     7621 cttagaattg aaattctctg ctaatttgga acaatgggat agtgtatgta agggcgacta
     7681 tctcattgaa aggggaggtg gagtataccc gcagaatgct gattttgatg ctagttgtat
     7741 tttcacggct gctccaataa ttgaagatga taagatttgg ttctactata tgggtggaaa
     7801 tggtaatcac actggcttcc gagaaagttc attagctaga ggatatatta agaaagacca
     7861 atttgcttat tatactaata agagagaagg aattccagct aaaatatcta cagttagctt
     7921 ccaaacttac gatgatgaaa tttatatctt agctgatatt cctgaaaatg gttatattaa
     7981 agttgcggta ggtactaaat acggtaatgc ttattcagat ttcggtcagg aagatatctc
     8041 tcttgttcaa gtttccgatg gtaagtataa gttggaattt aataaaaaat ctatcttaga
     8101 tttaggtaat tctccagttg cgttcaaatt tgaaatcgag aatgcaaaat tgtatggtat
     8161 tgaaggtagt attcagcaac actatctcaa atattaacca gaataattcg ttaacaaata
     8221 atctctatat tagtggagat agatatataa aatggaggtc ttattatggt aaagcttaat
     8281 aaggtattaa aagggagtgt tgcattcctg agtgcaagtt tattgctagc agcttgtggc
     8341 aataacgcta attcaaagcc cgctgaaaaa acagctgatg gtaaaacgaa aatttcgttt
     8401 ctatctacat ggcaaattaa tgatgttcgt ccgcctaaag atgaaacact tgttggtcaa
     8461 tggttagaag aacagctagg tattgatatt gatcttgtaa ctattcctgg cgagggagta
     8521 agttctaaag taaattctct tattactagt aaccagttac cgactgtttt gttgacaaca
     8581 ggagataaga gtgatatcgc tggtatcaac aaacttggta aacagggtgc attcttagat
     8641 ttgtcacaac acatggacaa actaccaaac tataagaagt accttgaaaa aaataatgca
     8701 ttagctcaaa tagaagatga tgaaaaacat atttatgctt tcacaaaatt ctttactgat
     8761 gagaatatca tgtatactac accaattctg agaaaagact tacttgttgg ttcagaattt
     8821 gaagatcttt caaaaattaa aactgttgat gactatacaa aagttttgaa atatttgact
     8881 gaaaaacaag gttctccagc atttattcaa cgtaatggct atgaaggctt catgaagcga
     8941 gttacaccat tgtggaattt aagccatcgt acatactatg actatgaatc agattcatat
     9001 aaacatccag ttgaacaacc tcaattgaaa caattcgttg aatggttgaa agaattacgt
     9061 aaagataacg tacttcaccc tgactgggca gttatgaaag atgaaacttg ggaaggtcta
     9121 ttggcttcta acaagggttc attcactgtt gaccgtatga atatcattgg agataataac
     9181 tttgataaat catttgattg gcagccactt gtttatccag aaattaatgg aaaacgttat
     9241 ttgcaaccga aaacaccgta catttctgat tcaggttggg taatcaatgc caatgcacca
     9301 aaagaagaaa ttgataaagc gttagaattc tttaatttct tatacgctga tgaaaatcaa
     9361 gaaaaacttg ctattggatt tgaaggtaaa acatttacac gtgaaaaccc taatactcaa
     9421 gcaggtatta aatggttgat tcaaatttac ggtgaaaatg ctggaaatga taaagcagaa
     9481 ctgctgttta aacatggtaa ccaagcattt actcgcgttc aaactaaatt agatattgag
     9541 gcacagccag gaacattccc taaagttatg tatgatcaag tagataaaat taagaaagat
     9601 ttaggtggtt ttagaccaga tactccggtt ctttccttta caactgaaga attagatgtt
     9661 ttaactaaat ctgaagcagg attaaatact ttctttgatg aaaatattat taaattcatt
     9721 gaaggacaaa gagatatgag tgagtgggat tcatttatca atgaagctaa atctaaagac
     9781 ttggataaag ttacaaaagt atataatgat gcctacaaac gtgtcaaaga tagaatgaaa
     9841 tagactaaat ggtagctctc tggtttttca gagagctact actgcctgag ggggagtaag
     9901 ctttgtatga tgtagtaatt ataggtgcag gaagttcggg cgcattggct gcaattgctt
     9961 cggggcgtct agggttaaaa acttgtctaa tagagaaggg aagtagaatt ggtggagttc
    10021 ctattaatac actaatgggg tcatttgcta atctatattt tgatgattct ggtagagtaa
    10081 atgcaagtaa aattgttggg gaattaatag aacgtattat caaaaaaggt ggtactgtct
    10141 ttaactctat ggaagaattg atgaatgtaa atgattttta tcagattaca attccctatc
    10201 aaccagaagt ttatgaatcg gttttaactg atatgttgat tgagtctaaa gttgatattt
    10261 tactaaatgt tgaagttaat aatattattt atgatagtaa aaatattaaa agtcttgaaa
    10321 ttttaagtaa tcaggaagtt aaattgattg attctaaagt ttttattgat gctacaggga
    10381 atgcaacaat tgcttctgct agtggagctg atgtttatga aaaaaattct tcttacggtt
    10441 gtttaatgag agttggagga gttgatatct caaagactat agaacatatt gaaaaaacaa
    10501 agcagtggaa ctatatagaa ggatataatc agtggttaga agaagtatta gcaaatcgaa
    10561 agtacagagc cactggagca gttttaaaag atccagtttc ttatgatcat gcccctatgc
    10621 tttcaaaaga cgaccatttt atgaatgatg aaaaatggaa ttacattaaa gaaagatggg
    10681 aaaaatttag atttatttac actttagaag tatcactatt taggaatgaa ttaaaaaaag
    10741 ctgtagaatc tggggatttt aattttgaaa tttatcatga tgataaatct ggagtcacta
    10801 tgaatggcga cggtataagc tatggttcat ggggtcaaaa tgtagcatta attaatgttg
    10861 caaaagcatt tggattcaat cccaaatata tggaagatga atcacatgct aatattcttt
    10921 ctagaaaata taatttaaca tttttatctt ttcttaaaaa atatgtacca ggttttgaaa
    10981 attcttttat ccttgatcag ggaactagag ctactaacag atcatgtagg cacattgata
    11041 attcatctat gatggacgaa attcttgatt ttccaatgta tacatttaag actatgtata
    11101 gctatcaaac tcctaaagaa ataacgtaca aatcaattgt aggtggaaaa ttgaataatt
    11161 tgtttgtaat aggtaaaggt gcttaccaat ccgaaagttt tagatcacaa attagttgta
    11221 tgttaatggg gatatcatca gcagcagcgg caaaaactat tattgaagat aaaagcaata
    11281 ctctaaacat taatagagat ttgtttaaat cgaacttaga tatattattt acatgggagt
    11341 tttctaattg gattactgat taacaaataa taagggaata ttaaatgaat gatactaata
    11401 aaaatcaata ctttgtttat gaatttgata ctagctttgt agaaatgtct tattctcagt
    11461 tttttgaagg atattgtatt cttttttcaa aagaacccta tccttcttta tctgatatgc
    11521 caatggaatt tagaaaagaa tttttaatgg aaatgagtat attaggtgat gcgatgcttg
    11581 aagtgttaaa tgcctataga attaattatg atatcttagg aaattcaaca cctgtacttc
    11641 atgctcattt atttccgaga tacatgtggg aagaggaaga gagaagaaaa actgtagttc
    11701 atagatatga ttcttcagtt tttagcaatc ctgagtttga atttactata gaaaagcatg
    11761 gaccaattat taaaaaactg aggtctgcta ttgatagaca ttatagagag tatcaacaat
    11821 tattagatga ttcaaactga actccacatt ttcgattaga tttaatacct tgtatattac
    11881 aactaggttg tggatgatat aattttttga aaaatatatt ttaggaatga cttataaagc
    11941 aattatatca gaggctatta aatcaatggc caaagcttat gctccttatt ctgattttaa
    12001 ggttggagct gcgttagtaa ctgaagataa aaaatttttt ctggatgtaa tatagaaaat
    12061 gctgcttttt ctcctacttg ctgtgcagaa cgagtatctt tttataaagc tgtatttaaa
    12121 ggagaacgtt cgttttcaag aacatgtatt gtaggtggaa aaaatggtat cttatcatct
    12181 tttatagcac cttgcggagt gtgtcgccag gtaatgatag aatttgtaat cctgattcat
    12241 ttgaagttat attggctagt gatgttgata cttataaagt atataaactt aaagaactac
    12301 taccagttag ttttaatcaa gaaaatttat aacaggaggg aactatggaa ttagttgaat
    12361 ttaaaaatgg acatgttaat acggttcatg agcatatcgc aataacgaaa gtaaaagagt
    12421 ttctaaataa tcatgatata ttaaaagata aaggtggcat acataaaatt tctactgatt
    12481 tcttttacaa tataattgat atggaaacta caactgaaga gaatagaccg tgggaaagtc
    12541 ataaggaata ttatgatgtc cattatctgt taaatggaga agaaacaatt ttatataact
    12601 tcttgtctca aatggaattg tctgaatata aagtagatga tgattggcaa caaatgaatg
    12661 ggacagcttt atttagtatt aaattaaaga aagatatgtt acttttgttg gaaccaaacg
    12721 atgcacatag aacaggccta ttggttgaag aaccacttaa tattaagaaa gttgttttta
    12781 aggtaaaaat ttaactgaaa gtactttcgg ggattaagaa ttttaaaata tttaagaaat
    12841 tgtattctaa agttctaaaa ttatagatat ttaatttaaa gcttgttaaa atagaatagt
    12901 aacactcatc tgctttcaaa aagcattcta ttccatctcc gattaacgat ggactttatc
    12961 acctccttct ccagtccttg tatgacatct tggagttgat tcatgacatc tt
//
LOCUS       DAVBCB010000056        12573 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVBCB010000056 DAVBCB010000000
VERSION     DAVBCB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.056"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(251..433)
                     /locus_tag="VQU28_001726"
     CDS             complement(251..433)
                     /locus_tag="VQU28_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477042.1"
                     /translation="MAVIFKNVLIEEKTEEEKQHILSILNGEYDLSDDVSCRLRRLNE
                     ESKRLEVFVKETNSLV"
     gene            complement(649..1881)
                     /locus_tag="VQU28_001727"
     CDS             complement(649..1881)
                     /locus_tag="VQU28_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002992300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HET2477043.1"
                     /translation="MKIAVAGTGYVGLSIAILLAQHHQVVAVDIIPEKVELINKKKSP
                     IQDDYIEKYLAEKELNLVATLNAEEAYENADFIVIAAPTNYDPKKNFFDTSSVEAVIE
                     AVMKYNPDAIIIIKSTVPVGYTKSVRRKFLTDNIMFSPEFLRESKALYDNLYPSRIII
                     GTDKDDKDLVKSAEIFVKMLQEGAVKENIETLYMDFTEAEAVKLFANTYLALRVSYFN
                     ELDTYSEMKGLDTQAIIDGVCLDPRIGSHYNNPSFGYGGYCLPKDTKQLLANYSDVPQ
                     EMITAIVQSNRTRKDYIADRILDLAGSYVGNDFYKPDKEKEIVVGIYRLTMKSNSDNF
                     RQSSIQGIMKRLKAKGVTIIIYEPTLESGTTFYGSQVVNDFEIFKKSSQVIVANRFDE
                     TLEDVRGKVYTRDIFKRD"
     gene            complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQU28_001728"
     CDS             complement(1910..2980)
                     /gene="gla"
                     /locus_tag="VQU28_001728"
                     /EC_number="5.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate 4-epimerase Gla"
                     /protein_id="HET2477044.1"
                     /translation="MRGKTILITGVAGFIGSNLVRKLLELEGTMTIIGLDNLNDYYDI
                     ALKDYRLLQLNKLIQDYPEKNWIFIKGDIADKMLINKIFKSYHPQIVVNLAAQAGVRY
                     SITNPDVYIESNIVGFHNVLEACRHSYEIYDGGVEHLVYASSSSVYGGNKKIPFATED
                     KVDNPISLYAATKKTNELQAYAYSKIYNIPSTGLRFFTVYGPAGRPDMAYFGFTDKLR
                     SGSDIEIYNHGNCKRDFTYIDDIVEGIVRVMQSAPKKLVGSDNLPLAPYKVYNIGNSK
                     PENLLDFVDVLQQELIKAGVLPENYDFDSHKKLVPMQPGDVPVTYADTSDLERDFGFK
                     PKTSLREGLRKFAEWYKDYYNV"
     gene            complement(3048..4217)
                     /locus_tag="VQU28_001729"
     CDS             complement(3048..4217)
                     /locus_tag="VQU28_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HET2477045.1"
                     /translation="MRLSKKYWFALTSKFLLILLTLLSSIIINRGLGVEKKGDYAYVM
                     NLVEIFYILGGVGLGQAYSTFKRNHSNEYQNKFVFLTFIQGLLFGIIGLIIVLADVPI
                     DYGGVIIVLSSLSIMRTNLTMIAVIENSIKRNKIVLGVSVVYSVMLVLLLLFHLVSIE
                     MVFLVYAINEFLRIILFLYYYNMFPVFEHISIKEFFTIYKFGFLSMILTLLITFNYSI
                     DIIMLKQMSSSYNVGIYSVAVTFSNMFLMVPDSFKEILFGDSAKRTFEKNKAINAIKV
                     SFLFMILLIIGFLSFGEFAITLLYGSSYIKAFPITLVIFLGSLSMIFFKILQPIYISQ
                     GKQSVAIKILSVSALINISLNWYLIPNYQITGAAIASAISYTVCGLFFIIDYLRN"
     gene            complement(4403..5707)
                     /locus_tag="VQU28_001730"
     CDS             complement(4403..5707)
                     /locus_tag="VQU28_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HET2477046.1"
                     /translation="MKFSLQSNDIKNKIILLVGVVFSGTLILCSENNDVTIPLFSMIF
                     LSFMAMIIVKFNIFHPYVWYSSFFTIYSISYPLLYRMEYINYGYTKDLIFMQWLALAT
                     LLLTLPSKAIVLSERKINLNNNNYLLFLNNMNTFITTVAIIYLLGSGFRNKGEIYSGA
                     NIVIMLVFSLVYFMILIHTYQLFIQLSTKKWRLLNCNTNTAIVICLFSIVTGERDYMF
                     TLFILDVIILFYFHKIKRFYLVMLVPLAALLIPLSAVFKYTLLSGQVSSINTNNIWFD
                     LLDGEFVSASRNLQILILHNMGNYFEGRSFFNDIVRIFYNTGYSNQTWFMDTFFPNVH
                     STKYGFTLVGEGYVNGGYFGIVMIFMLTGFLMRFLYINAQRNIYGMLIYLYMIPIFIY
                     STRADFANILSPLLKYAILGTLVIVFINRMVLISPLDEMGGE"
     gene            complement(5824..6978)
                     /locus_tag="VQU28_001731"
     CDS             complement(5824..6978)
                     /locus_tag="VQU28_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2477047.1"
                     /translation="MKVFIATEFRCTIYKNEIYLKPKAYFIYERYAKAFGEIVLCSRY
                     EKVESYPEGYHKANFINKFIPIEGLHQVLFGQNKNKIIEGMIDSDLIVVRIPSIIGSK
                     TADYALKIGKPYLTEIMGDAWDSYWYHSLKGKLLAPYIYAKTKSIVKNANYCIYVTEK
                     YLQDRYPNIKSNIVASNVNITSVENRSLKSRLYKLKKFNPQKISIMTTASVNVRAKGH
                     RFVLEAMKRLEIQGILLDYYLAGDGDQSFLKKKAEELGVANRIHFLGELTTSQIYEYL
                     DKVDLYIQPSLQEGLPRAVIEAMSRACPCIGSNKAGIPELLGPHCLFTPSSSQAIAGS
                     IIDFISMDKEKMVIKQFEFSKKFLSSVLNKRRSDYFAQIRRELNNSDGLD"
     gene            complement(6975..7526)
                     /locus_tag="VQU28_001732"
     CDS             complement(6975..7526)
                     /locus_tag="VQU28_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HET2477048.1"
                     /translation="MNRIIKNIVGLLRANICLILLKVCRNKDLTFHYFLKFGKNVTVE
                     QESNAKLILGKKIRVNAGGVLKVRKGAKLKISDDVFLSNNCMIACRKYIDIKSGVKCG
                     PGVLIYDHDYDVSVPGGLKAKKFKTAPVMIGENVWIGANSIVLKGVSIGENSVVAAGS
                     VVTKDIPADTIFIQKRLSREMKL"
     gene            complement(7519..8655)
                     /locus_tag="VQU28_001733"
     CDS             complement(7519..8655)
                     /locus_tag="VQU28_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HET2477049.1"
                     /translation="MNLQKKKIMLITNHDDDIYCFRKEVIESLVEYGYEVLVSCPYGD
                     KIELMKSIQFEYEDITIDRRGINPLKDIRLFLSYIRLLRKYRPDVVLTYTVKPNVYAS
                     LAATILGIKYINNVTGLGSVLTMGSCVRSLVLLLFRISFRKSNCVFFQNEYNMKMLKT
                     LGLILGDSKLIPGSGVNIEKYPVQQYPNGGNGIQGETIVFNFIGRILKEKGIDTYLAA
                     AQIIKSRYPKTEFNIIGFIEPTESNYELKICDLEKKGIVYYLGQQKDVIPHITRSHAI
                     IHPSVYGEGMSNVLLENASSGRVLITTDNPGCKEIVKDRETGYIFQGGNVEELVSILE
                     VFLGLENEKRKEMGLQGRMYIEQYFSRQIIIDAYLDKIKGALDE"
     gene            complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQU28_001734"
     CDS             complement(8796..9461)
                     /gene="cpsD"
                     /locus_tag="VQU28_001734"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HET2477050.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNTLCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIAMSFARAGYKTLLFDGDIRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTPPIGIVI
                     DAAIITQKCDASILITATGEANKRDVQKAKQQLEQTGELFLGVVLNKLDISVDKYGVY
                     GRK"
     gene            complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQU28_001735"
     CDS             complement(9471..10163)
                     /gene="cpsC"
                     /locus_tag="VQU28_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HET2477051.1"
                     /translation="MKEQNTLEIDVLQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYLVNRNQGEKPGLTNQDLQAGAYLVKDYREIVLSQDVLEEVISDLKLD
                     LMPKGLANKIKVTVPVDTRIVSVSVSDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIENALQMT
                     LLGVVPNLGKLK"
     gene            complement(10172..10903)
                     /locus_tag="VQU28_001736"
     CDS             complement(10172..10903)
                     /locus_tag="VQU28_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase CpsB"
                     /protein_id="HET2477052.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            complement(10905..12350)
                     /locus_tag="VQU28_001737"
     CDS             complement(10905..12350)
                     /locus_tag="VQU28_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HET2477053.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLICFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLLFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSKIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADS
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLTDGDRDRGRNQQKVIVAIIQKLTST
                     EVLKNYSSILQGLQDSLQTNIPIETMIDLVNTQLESGGNYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLTVVKAAIQDVMEGR"
BASE COUNT         4237 a         2433 c         1656 g         4247 t
ORIGIN      
        1 aattcactta cttcatatta catttagcat gaaatgaaat aagcgaattc tactttgttt
       61 gcatacaatt aaaaatcaat tcctaaggat gttttaaata actgtgtaaa aggcataatt
      121 tcaccatttc ttagtttttc aatgctaaat tacatagtaa ctggagagct attagaaatc
      181 tctaacttcc tcatagttaa aaaatgacaa tgagatactt attgatttca attcattaac
      241 agaatacgaa ctagacaagt gagttcgtct ccttaacaaa cacttccaag cgtttacttt
      301 cctcattcag tcgtcgtaat ctacaactta catcatcaga taagtcatac tcaccattta
      361 gaatagatag aatatgttgt ttttcttctt cagtcttctc ttcaataagc acatttttaa
      421 aaattactgc cattatcagc acctcacttc gaaggaatat atgcaactat atcctattaa
      481 gtcaatctct actgacaaaa gaagtatatt tacttcacta agtgcgagcg agaatataga
      541 aatatcttta ttgttttaga tgagttgtcg ctatcctcca gctagttttt aactattcta
      601 tcattcaaat gatatagatt attagggact agaaatatac aaattgattt aatctctctt
      661 aaaaatatcc ctagtataaa ctttacccct cacatcttct aaagtctcat caaaacgatt
      721 tgcaacaatc acttgactag atttcttaaa tatttcaaaa tcattaacta cttgactacc
      781 atagaaagta gtaccacttt caagagttgg ttcgtaaata ataatagtaa caccttttgc
      841 tttcaaacgc ttcataatac cttgaataga agattgtcta aaattatctg aattactttt
      901 cattgttaaa cgataaattc ctacaacaat ctctttttct ttatctggtt tatagaaatc
      961 attgcctaca taagatccag caagatctaa aatacgatct gcaatataat cttttcttgt
     1021 ccgattactt tgtactatag ctgttatcat ttcttgtgga acgtcagaat aatttgcaag
     1081 aagttgcttg gtatctttag gaagacagta acccccataa ccaaaagaag gattattgta
     1141 gtgagaacct attctcggat ctaaacaaac tccatcaata atcgcttgag tatctagtcc
     1201 tttcatctca gaataagtat ctaactcgtt aaaataggaa acccggaggg caagataggt
     1261 attagcaaaa agttttactg cttcagcttc tgtaaaatcc atatacaatg tttcaatatt
     1321 ttctttcact gcaccttcct gaagcatttt tacaaaaatt tcagcagact ttaccaaatc
     1381 tttatcatct ttatctgtac ctataataat tcgactcgga tataggttat catataacgc
     1441 ttttgactcc cgtaaaaact caggactaaa cataatattg tctgtaagaa atttcctccg
     1501 tactgacttt gtatatccaa caggaacagt acttttaatt attataattg catcaggatt
     1561 atatttcata actgcttcaa tgacagcctc gactgaagat gtatcgaaaa aatttttttt
     1621 cggatcataa ttagttggag ccgcaataac tataaaatcc gcattctcat atgcttcctc
     1681 tgcattcaat gtagctacga gattcaattc tttttccgcg agatattttt ctatataatc
     1741 gtcttggata ggggacttct ttttattgat taactcaact ttctcaggaa tgatatctac
     1801 ggcaacaact tgatgatgtt gagccagtaa gattgcaata gataatccaa catatcctgt
     1861 acctgctact gctattttca taattttttc ctttctcaaa acactctcgt tatacattat
     1921 agtagtcttt ataccactct gcaaactttc tcaaaccttc tcgtaaagaa gtttttggtt
     1981 taaaaccaaa atctctctct aaatcacttg tatctgcata agtaacagga acatcgcctg
     2041 gttgcatagg aactaatttt ttatgactat caaaatcata attttccggc aaaactcctg
     2101 ccttgattag ctcctgttga agaacatcca caaaatctaa caaattttcg ggtttactat
     2161 tcccaatatt gtacacttta tatggggcta acggtaaatt atcagatccc actagtttct
     2221 tcggagctga ttgcatgacc cttactatcc cctcaacgat atcatctata taagtaaaat
     2281 ctcgtttaca attaccatga ttataaatct caatatcgga accacttcgc aatttatctg
     2341 taaatccaaa ataggccata tctgggcgcc cagcaggacc ataaactgta aaaaagcgta
     2401 agcctgtcga aggaatattg taaatctttg aataagcata cgcttgaagt tcatttgttt
     2461 ttttagtcgc agcatatagt gaaataggat tgtctacctt atcttctgtt gcaaacggga
     2521 tttttttatt tccaccatat accgatgagg acgatgcata aactaagtgt tccacaccac
     2581 catcatatat ctcatacgaa tgacgacaag cttccaacac attatgaaag ccaacgatat
     2641 tactttctat atatacatct ggatttgtaa tggagtagcg tactcctgcc tgggcagcta
     2701 gattaacaac aatctgagga tgataactct taaaaatctt attaataagc attttatctg
     2761 caatatcacc cttgataaat atccagtttt tttctggata atcctgaatt agcttattaa
     2821 gttgaagcaa tcgataatct ttaagtgcaa tatcatagta atcattcaaa ttgtccaaac
     2881 caatgattgt catcgttcct tctaattcca ataatttcct aaccagattt gatccaataa
     2941 aaccagctac tcctgtaatt agaattgttt ttcctctcat atcataatac ctcttgttat
     3001 ttttatattt tcgtccacta atacacattc tttaacaact attataatca atttctaaga
     3061 taatctataa taaaaaacaa accacataca gtataagaga ttgctgatgc tatcgcagct
     3121 cctgtaattt ggtagttagg aattaagtac cagtttaaac ttatatttat caaagccgac
     3181 acagataaaa tttttatagc tacactctgt ttaccctgag atatataaat tggttgtaaa
     3241 attttaaaga aaatcatcga taagcttcct aaaaaaatga ctaatgttat aggaaatgcc
     3301 tttatataac tggaaccata aaggagcgta atcgcaaatt ctccaaaaga aagaaatcca
     3361 ataattaata aaatcataaa tagaaatgat acttttattg catttattgc tttatttttc
     3421 tcaaaagttc ttttagccga atcaccaaat aaaatctctt taaatgagtc tggaaccatc
     3481 aaaaacatat tagaaaacgt aactgcgaca ctgtatatac ctacattata actactagac
     3541 atttgcttca acattatgat atcaatacta taattaaatg taattaatag agtaagaatc
     3601 atagaaagaa atccaaactt atatatagtg aaaaattctt taatactaat atgttcaaaa
     3661 actgggaaca tattgtaata gtataaaaat aaaatgatac gtaaaaattc attaatagca
     3721 tataccaaaa ataccatctc tatagaaact aaatgaaata gtaacaacaa tactagcata
     3781 acggaatata ctacgcttac tcctaataca atcttattcc tctttatact attttctatt
     3841 actgcaatca tagtaagatt tgttctcata atagatagac tggacaaaac tattattacc
     3901 ccaccataat ctatcggaac atcagctaaa actattatta aacctattat tccaaacaat
     3961 aatccttgaa taaaagttaa aaaaacaaat ttattctgat actcattaga atgattcctt
     4021 ttaaaggtcg agtaagcctg acctaaacca actccaccta aaatataaaa aatttctact
     4081 aagttcatta cataggcata atctcctttt ttttcaacac ctaatcccct attaattata
     4141 attgaggata aaagtgttaa taagattaac aaaaatttac tcgttaaagc aaaccaatat
     4201 ttcttagata gtctcactta ttttctccgt atgttaaatt ttataacagt aaatttaaaa
     4261 ttatagtata ttgaaataga atatgaacaa aacgattagg gaatttaaag taatttataa
     4321 aaattcttta gaagctgcat tgtactactc caacttcaaa ccactatagt atcaaaaagt
     4381 ataccccacc tttttaaata aactactcac cacccatttc atccaatggt gatatcaaca
     4441 ccattctatt gataaaaaca ataaccaatg ttcccaatat agcatattta agtaatggag
     4501 ataatatgtt agcaaaatca gcacgagtag aataaataaa aataggtatc atatacagat
     4561 atatcagcat cccgtatata tttctttgag cgtttatata aagaaatctc atcaaaaagc
     4621 ctgttagcat aaaaatcatt acaattccaa agtatcctcc attcacatat ccctctccca
     4681 caagagtaaa accatatttg gttgaatgta cgtttggaaa aaaggtgtcc ataaaccaag
     4741 tttgattaga ataccccgta ttataaaaaa ttcttactat gtcattaaaa aaagaacgac
     4801 cttcgaaata attgcccata ttatgtaata tcagaatctg taaattacga ctagctgata
     4861 caaactcacc atctagcaaa tcaaaccaaa tattatttgt atttatagat gaaacttgcc
     4921 cacttaataa agtatattta aaaactgcag ataacggaat cagtagtgcc gcaagcggaa
     4981 ctaacattac aagataaaaa cgttttattt tgtgaaagta aaataatata atcacatcaa
     5041 ggatgaataa agtaaacata taatctcttt cccctgtaac gatagagaag agacagataa
     5101 ctatcgctgt gttagtatta caatttagta gtctccattt tttagtactc aactgtataa
     5161 atagctgata tgtatgaatg agaatcataa aataaaccaa agaaaatact aacattatca
     5221 caatattggc acctgaatat atttcccctt tatttctaaa accacttcct aatagatata
     5281 taattgctac tgttgtgata aacgtattca tattattgag aaataataaa tagttattgt
     5341 tatttaggtt tattttcctt tcgcttaaca caatggcttt agaaggtaga gttaacaaca
     5401 aagttgccaa agccagccat tgcatgaaaa tcaaatcttt tgtataacca tagtttatat
     5461 actccattct atagagtagc gggtaagaaa tagaataaat agtaaagaag ctactatacc
     5521 acacataggg atgaaaaata ttaaatttca caattatcat tgccataaaa cttaaaaaaa
     5581 tcattgaaaa taatggaatt gtaacatcat tattttcact gcataaaatt aaagtccctg
     5641 aaaaaactac acctactaga agtattattt tattttttat atcatttgat tgtagactaa
     5701 acttcattta cttttttcct ttaactaata atttaaacgt aatgcgtatt caaaaatttg
     5761 attggctatg tagtcagttt ttatagcata tttaatatat cgaaacggat tttccatcgc
     5821 attttaatct aaaccatcag aattatttaa ttctcttcta atttgagcaa aatagtcact
     5881 tctgcgttta tttaatacac tcgaaagaaa tttttttgag aattcaaact gttttatcac
     5941 catcttttcc ttatccatac taataaaatc tataatagat cccgcaatcg cttgtgaaga
     6001 ggatggagta aatagacaat gtggccccag caactctgga attcctgctt tattagaccc
     6061 tatacaaggg caagcacggc tcatagcttc aattaccgca cgaggtaacc cttcttgtaa
     6121 acttggttgt atatataaat caactttatc caaatactca taaatttgtg atgtagtcaa
     6181 ttcccctaaa aaatggattc tattcgctac tcccaattcc tctgctttct tttttaagaa
     6241 actttgatca ccatcacctg ctaaataata atccaacaaa ataccttgta tttctaatct
     6301 cttcattgct tccaatacaa atctatggcc cttggctcgt acattcacag atgctgttgt
     6361 cattattgaa attttttgag gattaaattt tttcaactta taaagacggc tcttcaaaga
     6421 tctattctct acagaggtaa tattaacatt tgaagcaacg atattagatt taatattagg
     6481 atatctatct tgtaaatatt tttctgtcac gtatatgcaa taattagcgt tttttacaat
     6541 tgatttagtt ttggcgtata tatatggagc taataatttt ccctttaaac tatgatacca
     6601 gtaagaatcc caagcatccc ccattatttc tgtcagatac ggcttaccta tcttcaatgc
     6661 gtagtctgca gtttttgatc caattataga cggaatacga acaactatta agtcgctatc
     6721 aatcattcct tcaataatct tatttttatt ttgaccaaat agaacctgat gtagaccttc
     6781 tatagggata aatttattaa taaagttcgc tttatgatat ccttcagggt atgattccac
     6841 cttctcataa cgactacata gtacaatctc tccaaatgct ttagcatagc gctcataaat
     6901 aaaataagct ttcggtttta aatatatttc atttttatag attgtacatc taaattcagt
     6961 cgctataaat actttcataa tttcatctcc cttgataaac gtttctgaat aaatatagta
     7021 tcagctggaa tatcctttgt tacaacactt cctgctgcaa ccacactatt ctcaccaata
     7081 ctcactccct tcaagacaat gctgttagct ccaatccaaa cattttctcc aatcataact
     7141 ggggccgtct taaatttttt tgctttcaat ccacctggaa cactaacatc atagtcatga
     7201 tcatatataa gtactccagg accacatttt actccagatt taatatctat gtatttacga
     7261 caagctatca tacaattatt actcaaaaat acatcatcag aaatcttgag ttttgctcct
     7321 tttctaactt tcaatactcc cccggcgttt actctaatct tttttcctaa gattaattta
     7381 gcattagatt cctgttcgac agttacattc tttccaaatt ttaaaaaata atgaaaagta
     7441 agatccttgt ttcgacacac ttttaataat atcaagcaaa tatttgcacg aagtaaacca
     7501 acaatatttt ttataatcct attcatctaa agctcctttt attttatcta aataagcatc
     7561 tataataatt tgacgagaaa aatattgctc tatatacatt ctcccttgaa gtcccatctc
     7621 ttttcgtttt tcattttcta gacctaaaaa aacttccaat atagagacta gttcctcaac
     7681 atttccccct tgaaatatat agcctgtctc tctatcttta acaatttctt tgcaacctgg
     7741 attatctgtc gtaattaaaa cacgtcctga actagcgttt tctagtaata cattgctcat
     7801 tccttcacca tacacactgg gatggataat tgcatgggaa cgggtaatat gaggtatcac
     7861 atctttttgt tgtcccaaat aataaacgat tccttttttt tctaagtcac aaattttaag
     7921 ttcataatta ctctctgtcg gttctataaa gccaataata ttaaactctg ttttgggata
     7981 tcgactctta ataatttggg cagcagccag ataagtatct ataccttttt cttttaatat
     8041 cctaccaata aaattaaaaa cgattgtttc tccttgaatt ccatttccac catttggata
     8101 ctgttgcaca ggatactttt caatgttcac tcctgaccca ggtatcagct tagagtcacc
     8161 caaaatcaag ccaagagttt ttaacatttt catattatat tcattttgaa aaaaaacaca
     8221 atttgatttc ctaaaagaaa ttctaaacag taataacacc aggcttctta cacaagaacc
     8281 cattgttaag acactaccta aacctgttac gttatttata tattttattc ctaaaattgt
     8341 tgcagctagg ctagcatata catttggttt cacggtatat gtcaagacta catccggtct
     8401 atattttctc aataatctta tataactaag aaatagacga atatctttta aagggtttat
     8461 tcctcttcta tcaatagtaa tatcttcata ctcaaattga atagacttca tcaactctat
     8521 cttatctcca taaggacatg acactagcac ttcataccca tactcaacta gtgactctat
     8581 aacttctttt cgaaaacaat agatatcatc gtcatgatta gttattaaca taattttttt
     8641 cttttgtaaa ttcatcaaaa taatcttcct aaaatataat taaccgctac tccattatcc
     8701 tttacaacta tcattttaaa aatagtaatt ttctataagt aattgtttcc aaattgtgac
     8761 actttaaaat tctatacaca tatagaactt acaaactatt tacgaccgta aactccatac
     8821 ttatcaaccg agatatccaa tttatttaaa acaactccca ggaacagttc ccctgtttgt
     8881 tctaattgtt gtttcgcttt ttggacatca cgtttattcg cctcacctgt tgctgttatc
     8941 aagatggacg catcacactt ttgagtgata attgccgcat caataacaat tccaataggt
     9001 ggtgtatcaa caatgatata atcaaaatat ttacgcaatg tttcaatcat atcattaaaa
     9061 tttttacttt gtaacaaggc tgtagggttt ggtgatacag atcccgattg aactacaaat
     9121 aaattttcaa tatttgtatc acataaaccg tgagataaat cagctgtccc agataaaaat
     9181 tctgttagcc ctgtaatttt ttcacgagat ttaaaaaatc ctgacataac tgaatttcga
     9241 atatcgccat cgaacaaaag agttttatag cctgcacgcg caaatgacat tgctatattt
     9301 acggaagtag ttgtttttcc ttcccccgga ttaacagaag taacggaaat tacttttagt
     9361 ttatctccgc tcaactgtat atttgtacac aaggtattgt aatattcttc tgccttctta
     9421 atgaactcca gttttttttg tgctatttct aatgtcggca tctctctctc ctatttcaac
     9481 ttacccaagt ttggtacaac tcccaaaagt gtcatctgca gtgcattttc gatatcttcc
     9541 ggacgtttca cacgagtgtc caaaagttca agaagaagaa ctataacact agttccaatc
     9601 acccctgcca aaaaaccaat tagtgtattg cgtttaatat ttggcgaaga cggtgatatc
     9661 gccggtcttg cctcctccag tgttgtcaca tcagaaacac gagtaatact gataattttt
     9721 tgagcagcta cttctcttaa agagttagcg atacggcttg cctcttcagg aactcgatca
     9781 ctaactgaaa cagagacaat acgggtatca actggtactg ttactttaat tttattagcc
     9841 aaaccttttg gcatcaaatc tagtttcaaa tcagaaataa cttcctccaa aacatcctgc
     9901 gataggacaa tttcacgata gtctttaacc aagtatgctc ctgcctgcaa gtcttgattg
     9961 gttaaaccag gcttctctcc ctgattacgg ttaactagat aaatccgagt cgtactagta
    10021 aactcaggtt tgataacaaa agtactgtag gcaaaagcaa ctgaagaagt tataattgcc
    10081 actaataaaa tgaccaactt tcttttccat aaagctctga atagttgcaa tacatcgatt
    10141 tccaaagtgt tttgttcctt catattttct cctaaattaa ttgatccatt ataatttttc
    10201 tgggattatc tacaaaaagt tctttcgctt ttttcgctcc atatttctta gcaatgatat
    10261 catatgcctg ttgcatatat ggaggtctac tgtctaaatt gtgcatgtca cttgcaacta
    10321 catgaactaa atcacgttcc aaaaaatacc gagctctctt tttcatgaat ttatattttt
    10381 caccaaagaa cttaggtttt gaaacatgat aactatttat ctgagtatag caccccatat
    10441 caatcagttc acgaacacgt ttttcgttat tctctaaagc atcataacgt tcaatatgag
    10501 caattactgg cgtgattccc aacatcaaaa tattgctcaa tcccgtatga atctgacgat
    10561 aggaagtatg catgctaaac tcaatcaagg cataacgact atcattaagg gtaggaattt
    10621 cttttttttc tagcttttct agagcatcca gagtatagta tatctctgcg ccataagcaa
    10681 tgactaaatc atctgctact tcttttgcaa tttcccgaac ctgaagaaag ttttctgcta
    10741 tcttctcttc cggagtttca aacatgccct tgcgacggtg agaggtagag acaatggttc
    10801 tcaccccctg cctgtaggat tctgccaaga gagccttgct ttcctctctt gacttgggac
    10861 catcatctac atcaaagacg atgtgcgaat ggatgtctat cattttatct accctccatc
    10921 acatcctgta tagctgcttt aactacagtt aaactactat catctatttc catcacatag
    10981 aggttactgt ctggcattgc ataagaagga agatccatcc gacctgtccc ttttaaatct
    11041 tgagaattta ctttataatt ccctccactt tccaactgag tattcactaa atctatcata
    11101 gtctcaatcg gtatatttgt ttgaagagaa tcctgcaatc cttgaagaat actactatag
    11161 tttttcaaaa cctctgtaga agttaacttc tgaataattg ctacaatgac cttttgttgg
    11221 ttgcgaccac ggtcacggtc tccatcggtt agtgagtagc gttcacgtac aaaacctaaa
    11281 gcttgctcag agtctagatg gacattccct actgggaaat ggaacttccc atgtagagct
    11341 gaaaactctt gatcattatg aacatctacc cctcccaata agtcaatcat tttcaagaaa
    11401 gaagtgaagt tcaatcgcac atagtaattg atatccactc catagagatt ttctaaggtg
    11461 tgaatggacg aatcaactcc ataaatgccc gcatgggtta atttatcttt ttgattattt
    11521 ccactatctg cgattggtac ataggcatca cgtggcgttg tggtcaagag gattttcttg
    11581 gtatctcgat tgacagtcat taggatattg acatctgatc gcgacaccga actaatagga
    11641 ccataggtgt caattccact aacatagata ttgaaagact gattcttaga cgtcttagga
    11701 gcctctactt ttttagtgaa tcccttggta taaatctttt ttatcttcga tgcgtagtct
    11761 ggatactctg actcgatgat attttcaaag acactattta ggacaatggc cttagtctcc
    11821 cctgcaatca aactcttgta agctgccaag taagacgaac tctggttgac cgtcaaatcg
    11881 gtattctgac ttgacttgat atcagctagt agtttttgaa tattttcatt atcagtccca
    11941 gtcggtgctg tcacactcgt cagttgcgta acattttcga tcttactatc tgctaaaaca
    12001 gcgacactga ttgaatattc tgagtaatta gaagtcgcat ttaaacgatt ggtcagtcca
    12061 acaaactgct gtactgcaaa gagcgacact gagctgacaa ggatagagaa cagcaacaga
    12121 aaaatagtaa acttttcagc ttttttatag ataatcaaga gtagccctac caaggcaact
    12181 agtaggacta acgcagttac cactaggtta agatatctaa aagcaaggat attgtactta
    12241 aagattaaga acaataaaaa acaaattaac aataaataaa tagtcagcaa aacgatatta
    12301 acacttcgct tcactttctg tgaacgtgat tttttaaaac gtctactcat gattaacacc
    12361 tataccttga acatcataca attatatcac ttttttacgg taatgtctac acctttattt
    12421 tcactatctg catctttaag tatcttagta gacttcccgc gaaccaaaaa tatagtaaaa
    12481 tgaaataaga ataggacaaa tcaatcagga cagtcaaatc gatttctaac aatgttttag
    12541 aatcagatgt gtactattct agtttcaata taa
//
LOCUS       DAVBCB010000057        11927 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVBCB010000057 DAVBCB010000000
VERSION     DAVBCB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11927
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.057"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            265..2556
                     /gene="pcrA"
                     /locus_tag="VQU28_001738"
     CDS             265..2556
                     /gene="pcrA"
                     /locus_tag="VQU28_001738"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HET2477054.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKSASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            2599..3279
                     /gene="radC"
                     /locus_tag="VQU28_001739"
     CDS             2599..3279
                     /gene="radC"
                     /locus_tag="VQU28_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HET2477055.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(3276..3965)
                     /locus_tag="VQU28_001740"
     CDS             complement(3276..3965)
                     /locus_tag="VQU28_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2477056.1"
                     /translation="MKKPVIGITGNEKAHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYNLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(3983..4624)
                     /locus_tag="VQU28_001741"
     CDS             complement(3983..4624)
                     /locus_tag="VQU28_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HET2477057.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(4707..4922)
                     /locus_tag="VQU28_001742"
     CDS             complement(4707..4922)
                     /locus_tag="VQU28_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HET2477058.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(4930..5277)
                     /locus_tag="VQU28_001743"
     CDS             complement(4930..5277)
                     /locus_tag="VQU28_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HET2477059.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFSLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(5282..6397)
                     /locus_tag="VQU28_001744"
     CDS             complement(5282..6397)
                     /locus_tag="VQU28_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HET2477060.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(6407..7366)
                     /locus_tag="VQU28_001745"
     CDS             complement(6407..7366)
                     /locus_tag="VQU28_001745"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2477061.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(7479..8048)
                     /locus_tag="VQU28_001746"
     CDS             complement(7479..8048)
                     /locus_tag="VQU28_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HET2477062.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAEDYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            8176..8847
                     /locus_tag="VQU28_001747"
     CDS             8176..8847
                     /locus_tag="VQU28_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HET2477063.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            8831..9649
                     /locus_tag="VQU28_001748"
     CDS             8831..9649
                     /locus_tag="VQU28_001748"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HET2477064.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            9646..10542
                     /locus_tag="VQU28_001749"
     CDS             9646..10542
                     /locus_tag="VQU28_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2477065.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEEGFETLEAIEHPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            10586..11560
                     /gene="pta"
                     /locus_tag="VQU28_001750"
     CDS             10586..11560
                     /gene="pta"
                     /locus_tag="VQU28_001750"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HET2477066.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3468 a         2281 c         2569 g         3609 t
ORIGIN      
        1 aaatcatcgt aaacgcttcg gattacgaat gaatttgatt gctggcatta tcaatcatga
       61 actaggattc tagttttgca ggaagtctat tgaggtattg agctagttta tgaaaaaatt
      121 gggtgaaaaa gtcgagtgtt ttagaaaccc acagtgtagt attctagttt caatccacta
      181 tattttgcta ctccccgtaa agtttctatt ttccctgatt tctgatataa tagaaatatt
      241 gacttcaaga gtaaggaaga gaagatgaac gcattattaa atggaatgaa tgaccgtcag
      301 gctgaggcgg tgcaaacgac agaaggtccc ttgctaatca tggcaggagc tggttctgga
      361 aagactcgtg ttttaaccca ccgtatcgct tatttgattg atgaaaagct ggtcaatcct
      421 tggaatatct tggccattac ctttaccaac aaggctgcgc gtgagatgaa agagcgtgct
      481 tatagcctca atccagctac tcaggactgt ctgattgcga ccttccactc catgtgtgtg
      541 cgtattttgc gtcgcgatgc ggaccatatt ggctacaatc gtaattttac aattgtggat
      601 cctggtgaac agcgaacgct catgaaacgt attctcaaac agttgaactt ggatcctaaa
      661 aaatggaatg aacgaactat tttggggacc atttccaatg ctaagaatga tttgattgat
      721 gatgttgctt atgctgctca agctggcgat atgtatacgc aaattgtggc ccagtgttat
      781 acaacctatc aaaaagaact tcgtcagtct gaatccgttg actttgatga tttgattatg
      841 ctgaccttgc gtctctttga tcaaaatcct gatgttttga cctactacca gcaaaaattc
      901 caatacatcc acgttgatga gtaccaagat accaaccacg ctcagtacca attggtcaaa
      961 ctcttggctt cccgttttaa aaatatctgt gtggttgggg atgcggacca gtctatctac
     1021 ggttggcgtg gtgctgatat gcagaatatc ttggactttg aaaaggatta ccccaaagct
     1081 aaggttgttt tgttggagga aaattaccgc tcaaccaaaa ccattctcca agcggccaac
     1141 gaggttatta aaaataataa aaatcgccgt cctaaaaatc tctggactca aaacgctgat
     1201 ggggagcaaa tcgtttacta tcgtgctgat gatgagctgg atgaggctgt atttgtagcc
     1261 agaaccatcg atgaacttag tcgcagtcaa aacttccttc ataaggattt tgcagttctc
     1321 tatcggacta atgcccagtc ccgtacaatt gaggaagccc tgctcaagtc taacattcct
     1381 tataccatgg ttggcggaac caaattctac agccgtaagg aaattcgcga tattattgct
     1441 tatctcaacc ttattgctaa tttgagtgac aacattagtt ttgagcgtat tatcaacgag
     1501 cctaaacgtg gaattggtct aggtacagtt gagaaaatcc gtgattttgc aaatttgcaa
     1561 aatatgtcta tgctggatgc ttctgctaat attatgttgt ctggtatcaa gggtaaggca
     1621 gcccaatcta tctgggattt tgccaatatg atgcttgatt tgcgggagca gctagaccac
     1681 ttaagcatta cagagttggt tgagtccgtc ctagaaaaaa caggttatgt cgatattctt
     1741 aacgcccaag cgactctaga aagcaaggca agggttgaaa atatcgaaga gtttctttct
     1801 gttacgaaga actttgatga caccactgat gtgacagaag aggaaactgg tctggacaaa
     1861 ctgagtcgtt tcttaaatga cttggctttg attgccgaca cagattcagg tagtcaggag
     1921 acatcagaag tgaccttgat gaccctgcat gctgccaaag gtctcgaatt tccagttgtc
     1981 tttttgattg ggatggaaga aaatgtcttt ccacttagtc gtgcgactga agatccagat
     2041 gaattagaag aagagcgccg tctagcctat gtaggtatca cgcgtgcaga gaaaattctc
     2101 tatctgacca atgccaactc acgcttgctt tttggtcgta ccaattataa ccgtccgact
     2161 cgttttatta acgaaatcag ttcagacttg cttgagtatc aaggtctggc tcgtcctgca
     2221 aatacaagct ttaaggcatc atatagcagt ggtagtattt cctttggtca aggtatgagt
     2281 ttggctcagg ctcttcaaga ccgtaaacgc ggtgctgccc caaaatcgat ccagtcaagc
     2341 ggtcttccat ttggtcaatt tacagctggc gcaaaatcag catctagcga ggcaaattgg
     2401 tccattggtg acattgctct ccacaagaaa tggggagagg gaactgttct ggaagtttca
     2461 ggtagcggtg ctaggcagga attgaaaatc aatttcccag aagtaggttt gaaaaaactt
     2521 ttagccagtg tggctccaat tgagaaaaaa atctaatttt ccatccttct cacgaataat
     2581 aaagtgagga ggatttttat gtacagtatt tcattccaag aagattcact attaccaaga
     2641 gaaaggctgg ccaaggaagg agttgaagcg cttagtaacc aagagttgct agctatttta
     2701 ctcaggacag gaacacgtca agctagcgtt tttgaaattg cccaaaaagt cttgaacaat
     2761 ctttcaagcc taacggattt gaaaaaaatg accctgcagg aattgcagag tttgtctggt
     2821 attgggcgtg ttaaggccat agaattacaa gctatgattg aactggggca tcgtattcac
     2881 aaacacgaga ctcttgaaat ggaaagtatt ctcagcagtc aaaagttggc caagaagatg
     2941 cagcaggaat taggggataa aaaacaagag cacctggtgg cactctatct caatactcaa
     3001 aatcaaatca tccatcagca gaccattttt atcggttctg taactcgtag tatcgctgaa
     3061 ccgcgagaga ttcttcacta tgcaatcaag catatggcga cttctcttat cttggtccac
     3121 aatcatcctt caggagcggt agcgcctagc caaaatgatg atcatgtcac taaacttgtt
     3181 aaagaagcct gcgaattgat ggggattgtt ctcttggacc atttgattgt ctctcattct
     3241 aattacttta gttatcgtga aaagacagat ttaatctaaa gttcattaac gacatagtca
     3301 aagagttttt tatctttggg acgattttca aaaagaagtt ctggatgcca ttggacaccg
     3361 agaaaggcga catcatccgt actcatgaca gcctcaatga taccatcttt aggatcatga
     3421 gccacaactt ttaaatttgg tgctaagtcc ttgatgctct ggtggtggaa ggagttgata
     3481 tgagagattt ctccatagat ttcttggaga acggtatctg gttctgttac caagcgttga
     3541 gttgtgtact caacagaaga atcctgccaa tggtcttcga tatcttggta caaagttcca
     3601 cccatggcaa cgttaaagag ttgggtacca cggcagacag agaaaatggg ctttttctgt
     3661 ttaatagctt ccttgatgag ggccagttcg aagatatctc tttgaaggtt atagtcatcg
     3721 ctatcaatgg ttttgggttc gccataaaat tttggatcga cattttgccc acctgtcaag
     3781 atgagcttgt caatcaaact gatatagtgg caggccattt cttgatcacc aatcggtagg
     3841 atgatgggaa tccctccagc atctttaacg ccttcaacaa agccttttgc tgcgtagctc
     3901 atcatgatgt catcatctgg atgagctttt tcgtttcctg taatcccaat aactggtttt
     3961 ttcataaaat gattttcgct ttctaatcct cttttcgcat gaagtagagg agggtttgga
     4021 gttcacttgt caaatcgaca tactgaacga ccacgtcttt tggtaaatgc agatggactg
     4081 gtgaaaaact gagaattcct ttcacaccag catcaaccaa gagattagca acctcttgtg
     4141 acttgacgct gggaacagtt aggatagcag tcttcacatc agcatccttg attttatcct
     4201 tgatctgaga aatcccgtaa atgggaatcc cgtcaggagt ttgggtaccg acttcaggat
     4261 ggtcgtctag gtcaaaggcc atgataatct tcatcttgtt acgttcgtgg aagcggtagt
     4321 ggagaagggc atggcccata tttccaatac caaccagcat gacattggta atagagttgt
     4381 cattgagcaa atcggcaaaa aatgtcatta gttttttgac atcatagcca aaaccacgac
     4441 gaccaagttc accaaaatag gaaaaatcac gacgtacggt cgctgaatca ataccgatag
     4501 cctctgcaat ttgcttagag ttggcacgtt caatcttttc tgcatgaaat ctcttaaaaa
     4561 ttcgatagta gagagagagt ctttttgctg tagcttttgg aatagcaaac tgtttatctt
     4621 tcacaaaatc acaacctttc tattcttcta ttttatagaa acattgtgaa aaaatcaaca
     4681 aaaataagaa aaaactaaga aaaatcttag ttttgatgta aaaaatctgc atgagataga
     4741 aaacggtaga ggtctccgac cagaccctga taaacttttt ggcccctaaa agtcagagaa
     4801 gtcacataaa gtgtatctgg taaggttaca catcctgaca aagtcaacat gagagcctca
     4861 tgatcctcat acttgagagt acgctctaca tgatagcagt ccttataggt cagttcaaac
     4921 attttggctc tatctttccg attttgtaaa gacaccacgt tctaccaagc tatccatgag
     4981 gaagtagaat ttttcctgat gaatatggtg gtcttctgat ttgaaaatat caactagacg
     5041 aaggctaaac ttgtcagtga tattgatttt agcccctgta agttccttgt taatgatgat
     5101 tttgagttgg aagccttcac cgctgtttgg cactttttcc aaaaggcgag tcagttcata
     5161 gttaccaacc ttagtttcaa aaaaggtgtt atctttgagg gtgaattttt taacagaagg
     5221 gctaagagtg taatcgtaac gacaattttt taactgaatg attttttcaa atgccatatg
     5281 gctaacctcc gataatttct tttaaggttt ttgcgagggt ttgtaggtct tcaacggtat
     5341 tttgtggcga caaactgatg cgaagggatt ccttcaagcg ttctgaattt gcgccataca
     5401 tggcttcaag aacatggctg gattggacaa cgcctgcagt acaggctgag ccagtagaga
     5461 ttgaaattcc agctaaatct agccgaagga gtaagaggtc atttttctga ccaggaaatc
     5521 caatattgag aacataaggg agatgatgtt ttcctctatt caagtaatac tgaatgccct
     5581 ccagctctgc cagaaaggcg gtttctagat tttgtacatg ttgaaaatgt tcttcttgtt
     5641 tttctaggtc ttcttttagg gctgcaacca tgcctacgat agcaggcaga ttttcagttc
     5701 ctgcacgttt tttctgttcc tggtctccgc catgtagata ggaatcaaag tccatgctag
     5761 atgcgtagag aaaaccgatt cccttaggac catggaattt gtgggcagaa gcagtgagaa
     5821 aatcaatgcc caattcttct gaatgaattg ggattttacc aatagcctga actgcatcaa
     5881 catgataggc agcagggtgt tgcttgagta tttggccaat ttcagcgatg ggcagtaggt
     5941 ttcctgtctc attattggca aacatggtag aaaccaaaat cgtatcgtca cgtaaagcct
     6001 tttgaatttg ctgggctgtg atttcttgat tttctggctg gataatggtt gcttcaaacc
     6061 caaagtgttg aaccaagtaa tcaattgttt caaggacagc atggtgctcg atggcagttg
     6121 tgatgatatg ttttccttgt tcttggtgac gaagacagta gccaatgatg gtagtattat
     6181 tgccttcagt cccaccagaa gtgaaaaaga tatgttgagg ttttgtcctt agtaactggg
     6241 ctagttcctg acgggcttct cgcaagagtt tgccagcttg acgaccatga ccatgaatac
     6301 tagaaggatt tccgtgggtt tcttgcataa ccttggtcat agctgaaata gcaactgctg
     6361 acataggagt cgttgcagca ttgtccaaat aaatcaaaga atcaccttat ttctttttat
     6421 tgtaggcaaa gagtgggctg actggttttc tttcgtgaat acggacgata gcatcaccaa
     6481 ttaactcact agcagtgatg tagcatacat ttttaggagt tttttctttt gttgctactg
     6541 aatcagtcac aagaatttct ttaatattag tattgtcaag aagctcagca gctccctcga
     6601 cgaagagacc gtggctagaa acagcataaa tttctgtagc tccttcacgt tcaacgattt
     6661 tagaagcttc agagaaggta cgtcctgtat ttaaaatatc atcaatcaag atagctttct
     6721 taccttcaac atcaccaata atataacctt cgttacgagt tgcatcgtct tgagggtagt
     6781 cgataatggc gataggagca tcaagatatt cagccaggct acgcgcacgt ttgacacctg
     6841 aatttttagg gctaacgaca acaacatctg aaccaagcaa tcctttatcg cagtaatgtt
     6901 ttgcgaatag gggaacagtg aaaagattat ccactggaat atcaaagaaa ccttgaacct
     6961 gaacggcatg caaatcaaga gtcaggatac gatcaactcc agccttaacc agcatattgg
     7021 caactagttt tgctgtaagt ggctcacgag gacaagcaat gcggtcttga cgtgcatagc
     7081 caaaatatgg aaggacaacg ttgatactgt gggcacttgc acgcacacaa gcatcgacca
     7141 tgattaacaa ttccattagg tggttgttga cagggaaact tgttgattgg atgatgtaaa
     7201 catcataacc acggacactt tcttcgatat ttacttggat ttctccgtct gaaaattgac
     7261 gtgatgatag ttttccaagt gggacaccaa cagcttgggc aattttttgt gcaatctctt
     7321 ggttagagtt gagtgcgaaa agtttcatgt tttttctatc tgacattata gaccgtcctc
     7381 tgtaaacttt ataaatccta gttatattta ccttacatat atgaactggg atttgtgtat
     7441 ttttatcttt tctattttac caaaaaatgg agattatttc agctattttt catacttttg
     7501 acaaatcgaa ccaattttga aggagctttt tggtaggaaa tctgattttt ctctaaaaat
     7561 tgtcggaaat cctgtttgcc ttgctcatga ttttccactt caagctccaa ttcgtaatct
     7621 gttatatcaa agtatcggct ctgatccagt gccatgagac caatagctgt tttcatttca
     7681 tagcgaagcg ttgttagaca accaagaacc tgccagttct tactttggat accatgtttc
     7741 gccaattcat ccagtactag tccttgagga agttcttcct tactcagata gtcctcagca
     7801 tcttttagtt gcaatttttg gttgtattcc atgtttccaa cactctgcgg gactttgagt
     7861 gtcaactcag cccagtcttc aaaggttcga atgcgcatag cgactttctt ttctcgcagt
     7921 tcaaaatcag gcgtgtcgat gtagtaattt gtttgaagaa caggagtgac acctgtgaac
     7981 tggtctttta gacgattgta ttcatctttt ttcaatagtg ttttcaattc aatttctaaa
     8041 tgtttcattt ttcttacctt tttttatcgt tgaaagcgga tttatggtat aataagcatt
     8101 gtatttattg tatatgaatc tggagaaaaa atcaaagata tttttgacgg ataatatgag
     8161 aacaagggag aatatatgac cttagaatgg gaagaatttc tagatcctta cattcaagct
     8221 gttggtgagt taaagattaa acttcgtggt attcgtaagc aatatcgtaa gcaaaataag
     8281 cattctccaa ttgagtttgt gaccggtcga gtcaagccaa ttgagagcat caaagaaaaa
     8341 atggctcgtc gtggcattac ttatgcgacc ttggaacacg atttgcagga tattgctggc
     8401 ttacgtgtga tggttcagtt tgtagatgac gtcaaggaag tagtggatat tttgcacaag
     8461 cgtcaggata tgcgaatcat acaggagcga gattacatta ctcatagaaa agcatcaggc
     8521 tatcgttcct atcatgtggt agtagaatat acggttgata ccatcaatgg agctaagact
     8581 attttggcag aaattcaaat tcgtactttg gccatgaatt tctgggcaac gatagaacat
     8641 tctctcaact acaagtacca aggggatttc ccagatgaga ttaagaagcg actggaaatt
     8701 acagctagaa tcgcccatca gttggatgaa gaaatgggtg aaattcgtga tgatatccaa
     8761 gaagcccagg cactttttga tcctttgagt agaaaattaa atgacggtgt aggaaacagt
     8821 gacgatacag atgaagaata caggtaaacg aattgatctg atagccaata gaaaaccgca
     8881 gagtcaaagg gttttgtatg aattgcgaga tcgtttgaag agaaatcagt ttatactcaa
     8941 tgataccaat ccggatattg tcatttccat tggcggggat ggtatgctct tgtcggcctt
     9001 tcataagtac gaaaatcagc ttgacaaggt ccgctttatc ggtcttcata ctggacattt
     9061 gggcttctat acagattatc gtgattttga gttggacaag ctagtgacta atttgcagct
     9121 agatactggg gcaagggttt cttaccctgt tctgaatgtg aaggtctttc ttgaaaatgg
     9181 tgaagttaag attttcagag cactcaacga agccagcatc cgcaggtctg atcgaaccat
     9241 ggtggcagat attgtaataa atggtgttcc ctttgaacgt tttcgtggag acgggctaac
     9301 agtttcgaca ccgactggta gtactgccta taacaagtct cttggcggtg ctgttttaca
     9361 ccctaccatt gaagctttgc aattaacgga aattgccagc cttaataatc gtgtttatcg
     9421 aacactgggc tcttccatta ttgtgcctaa gaaggataag attgaactta ttccaacaag
     9481 aaacgattat catactattt cggttgacaa tagcgtttat tctttccgta atattgagcg
     9541 tattgagtat caaatcgacc atcataagat tcactttgtc gcgactccta gccataccag
     9601 tttctggaac cgtgttaagg atgcctttat cggtgaggtg gatgaatgag gtttgaattt
     9661 atcgcagatg aacatgtcaa ggttaagacc tttttaaaaa agcacgaggt ttctaaggga
     9721 ttgctggcta agattaagtt tcgaggtgga gctattctgg tcaataatca accgcaaaat
     9781 gcaacgtatc tattggacgt tggagactac gttaccattg acattcccgc tgaggaaggc
     9841 tttgaaacct tggaggctat tgagcatcca ttagatattc tctatgagga tgaccacttt
     9901 ctagtcttga ataaacccta tggagtggct tctattccta gtgtcaatca ctctaacacc
     9961 attgccaatt ttatcaaggg ttactatgtc aagcaaaatt atgaaaatca gcaggttcac
    10021 attgttacca gactagatag ggacacttct ggcttgatgc tctttgccaa gcacggttat
    10081 gcccatgcac gattagacaa gcagttgcag aagaaatcta tcgagaaacg ctactttgct
    10141 ttggttaagg gagatggaca tttggagcca gaaggggaaa ttattgctcc gattgcgcgt
    10201 gatgaagatt ccattattac cagacgagtg gctaaaggcg gaaagtatgc ccatacttca
    10261 tacaagattg tagcttctta tggaaatatt cacttggtct atattcacct gcacactggt
    10321 cgaacccatc aaatccgagt ccatttttct catatcggtt ttcctttgct gggagatgat
    10381 ttgtatggtg gtagtctgga agatggtatt caacgtcagg ctctgcattg ccattaccta
    10441 tccttttatc atccattttt agagcaagac ttgcagttag aaagtccctt gccggatgat
    10501 ttcagtaacc ttattaccca gttatcaact aatactctat aaaaactgtc tcagagtata
    10561 attattatct taaaggagaa aactcatgga agtttttgaa agtctcaaag ccaaccttgt
    10621 tggtaaaaat gctcgtatcg ttctccctga aggggaagag cctcgtattc ttcaagcaac
    10681 aaaacgctta gtaaaagaaa cagaagtgat tcctgttttg cttggaaatc ctgaaaaaat
    10741 taaaatttat cttgaaattg aaggaatcat ggatggttat gaggtcatcg accctcaaca
    10801 ttatcctcaa tttgaagaaa tggtttctgc cttggtggag cgtcgcaagg gcaaaatgac
    10861 tgaagaagat gtacgcaagg ttttggttga agatgtcaac tactttggtg tgatgttggt
    10921 ttacttgggc ttggttgatg gaatggtgtc aggagcgatt cactcaacag cttcaacagt
    10981 tcgtccagct ctacaaatca tcaaaactcg tccaaatgta actcgtactt caggagcctt
    11041 cctcatggtt cgtggtacgg aacgttacct atttggagac tgtgccatta acatcaatcc
    11101 agatgcagaa gccttggctg aaattgccat caactcagca atcacagcta agatgtttgg
    11161 catcgaacct aaaattgcca tgttgagcta ttctactaaa ggttcagggt ttggtgaaag
    11221 cgttgataag gtcgttgaag caactaaaat tgctcacgac ttgcgtcctg accttgaaat
    11281 cgatggtgag ttgcaatttg atgcagcctt tgttcctgaa actgcagctc tgaaagctcc
    11341 tggaagtacg gtagctggtc aagcaaatgt cttcatcttc ccaggtatcg aggccggaaa
    11401 tatcggttac aagatggctg aacgcctggg tggctttgcg gctgtaggac ctgttttgca
    11461 aggtttaaac aagccagtta atgatctttc tcgtggatgt aatgcagatg atgtttacaa
    11521 gttgaccctc atcacagcag ctcaagcagt tcatcaatag tgaaaactat aaagcgatat
    11581 actatgctat actgtagtta tgaaactatg tacgaaaagc actgccatta attcctgaga
    11641 actaaattac tgattggtgt caaaaaggaa aacttccaag cgatgatatc ctgtctatac
    11701 acgacctata gaaatctgta atatgtatgt ccgtaaaacg ataatttccc tttttgattt
    11761 taaatgagta tgaaaagaga attttttcat gagaaaacgc ataatattaa gtgtcttgac
    11821 acctgctatt atgcgttttc tggctctttg tcaactgtag tgggttgaag aaaagctaag
    11881 ctcgagaaag gacaaatttt gtcctttctt ttttgatatt cagagcg
//
LOCUS       DAVBCB010000058        11679 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVBCB010000058 DAVBCB010000000
VERSION     DAVBCB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.058"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..1017)
                     /locus_tag="VQU28_001751"
     CDS             complement(<1..1017)
                     /locus_tag="VQU28_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HET2477067.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMKELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     KGEILEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESSEKFEAIKQNLVDEMWKVAQRELATGFYYGTPSENEQLFGARRKIPEYKFVAEVV
                     SYDDA"
     gene            1641..1823
                     /locus_tag="VQU28_001752"
     CDS             1641..1823
                     /locus_tag="VQU28_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2477068.1"
                     /translation="MANDNKSHYLIYRVLGISFEEGENIDLYQNKGRFLYKYAGSFLE
                     EAAVLSFNEKFGTENT"
     gene            1833..2198
                     /locus_tag="VQU28_001753"
     CDS             1833..2198
                     /locus_tag="VQU28_001753"
                     /inference="COORDINATES: protein motif:HMM:NF021048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
                     /protein_id="HET2477069.1"
                     /translation="MPNSENTKPKTFEIDCLVGEKHAYEIKWWDATTDGDHITKEHTR
                     IKVIHNKGYIPIRLMFYYPNRTQAIKIQQTLETLYNGIGGKYYYGDSAWEHLRAVTGI
                     DLLSILTDIANKKTGVKSK"
     gene            2195..3385
                     /locus_tag="VQU28_001754"
     CDS             2195..3385
                     /locus_tag="VQU28_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HET2477070.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RNGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(3900..4892)
                     /locus_tag="VQU28_001755"
     CDS             complement(3900..4892)
                     /locus_tag="VQU28_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2477071.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            5052..6806
                     /locus_tag="VQU28_001756"
     CDS             5052..6806
                     /locus_tag="VQU28_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477072.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISAVLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIVTPILVLVLGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAEVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNY
                     NIEFENVSFAYNDKAIIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            6790..8511
                     /locus_tag="VQU28_001757"
     CDS             6790..8511
                     /locus_tag="VQU28_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477073.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERIHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            8522..9151
                     /locus_tag="VQU28_001758"
     CDS             8522..9151
                     /locus_tag="VQU28_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HET2477074.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALIALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            9052..9609
                     /locus_tag="VQU28_001759"
     CDS             9052..9609
                     /locus_tag="VQU28_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HET2477075.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            9597..10961
                     /locus_tag="VQU28_001760"
     CDS             9597..10961
                     /locus_tag="VQU28_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477076.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDILEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKELSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKTSPFIFFDEPSSGIDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         4258 a         1670 c         1977 g         3774 t
ORIGIN      
        1 cgcatcatca taagaaacca cttcagcgac aaacttgtac tcaggaattt tacgacgagc
       61 accaaacaac tgctcatttt cagatggtgt accatagtaa aatcctgtag ccagttcacg
      121 ttgggcaacc ttccacatct cgtccactaa gttttgtttg atggcttcaa acttttcaga
      181 actttcaaga taggcatcca cagccgcctt gtagcagttg gttactgttg atacgtagtg
      241 aatagacttc atacgtcctt cgatttttag actgtccaca ccattttcaa tcatatctgg
      301 aatgtggtca atcatagaca tatcaacggc tgacattgaa aattcttcta gaatctcacc
      361 cttcaaactc ttacgttctt tcccaaatgg catatcgtaa aggtcgtatt tccaacggca
      421 tgactgagaa cacccaccac ggttggcatc acgcatactc atgtggtttg aaagagtaca
      481 acgtccagag tatgaaatac acatagctcc atggacaaag gcttcaattt caacatctgt
      541 acgtttgcgg atttcagcta attctttcat tgaaacctca cgtgctaaaa cgacacgagt
      601 caagcctagc tctttccaga actcaagggt ttcatagtta gtggcactgg cttgggtaga
      661 aaggtggatt tcaaggcctg gtgcttcagt cgctgcaatc ataatcaagg ctgggtcaga
      721 tacgataact gctgcaatcc cgatatcacg cagtttacga aaccactcac cagcaccagc
      781 ttcatttcct tcgtgcataa ccatattagc cgctacatag accttggcac catacttggc
      841 cgcaaactgc acgccttctt ccatctgttc gaaagtaaag tttcccgcac ggctacgaag
      901 accataggcc tgaccaccga taaagacagc atctgctcca tactgaacag ctacctttag
      961 cttctctaaa gtccctgcag gtgataaaac ctcaggacgt tttaatgttt ttgtcatgat
     1021 ttctcctatt tgcaatataa aagtttgaca tttttccttc tcattttata gtaaataaag
     1081 gataaaatca agcctagacg gattgttttg acaattctaa tcttttagac tttgcataat
     1141 aactattaaa cagtcgaaaa ttaccccagt tttaacaagt agtcaaaaag tctaaaaaag
     1201 taaaaaccag tatcccaaga tactgactcg taaaaatata taaattagtc cttagatgac
     1261 tactcccact ctgttgctcc tataatggaa aaggattgat acagtctaga atatcgcttt
     1321 tttatttatt attctgatat aaaactgtta gaattgattt tttgttttat acccaaatat
     1381 gaatagagac gtagctaata agttgaataa catatttgga aaaatgagaa atagcaatat
     1441 tactagaact tttagaaaca atcaatattt aaaaagaaaa aagctcaaag aatttattaa
     1501 accatgttat aatagatata gctataattt tatttataac aagaggatag aaatgaccga
     1561 attagaaaga aaaaatcgaa aaattagcta agaaatattc tgataactta aacatcaaag
     1621 ttcaagagag agttcgtgaa atggcaaatg ataataagag ccattatttg atatacagag
     1681 ttttaggtat ttcatttgaa gaaggagaaa atatcgattt gtatcaaaat aaaggtcgtt
     1741 ttttatacaa atatgctggt tcatttttag aagaagctgc agtactatcc tttaacgaaa
     1801 aatttggtac agaaaatact taaaaagtta acattcctaa ttctgaaaat acaaaaccta
     1861 agacttttga aattgattgt ttagtcggag aaaaacacgc atacgaaata aaatggtggg
     1921 atgcaactac agatggagac catataacta aagaacacac tagaataaaa gttattcata
     1981 acaaaggata tataccaatt cggttaatgt tctactatcc aaatagaact caagctataa
     2041 aaattcagca aactttagaa acattgtata acggtattgg agggaaatat tattatggag
     2101 attctgcctg ggaacattta agagcagtga ccggtattga tttacttagt attctaacag
     2161 atattgcaaa taaaaaaaca ggggtaaaat caaaatgaca gtattaaaag gagataactt
     2221 agaaatatta aaaactattg aatcctcaag tattgattta atctatatgg accctccttt
     2281 ctttacacag aaaacccaaa aattatctaa taacaaaaat attatgtatt cattcgaaga
     2341 tacgtggact tcgattgagg attacaaaga atttttgtct gtaagattag aagaatgcaa
     2401 aagagtgcta aaaaatagtg gcagtatttt cgttcattgt gataaaattg caaatcatca
     2461 tattagatta attttagata atatctttgg agcagatatg tttcaaagcg aaattatatg
     2521 gaactataaa cggtggtcta attcaaaaaa gggattattg aacaatcatc aaaacattta
     2581 cttttattca aagtcaaaag attttaaatt taatacaatt tttacagagt attcttctac
     2641 tacaaatatc gaccaaatac tagtagaacg aaaacgaaat ggaaactcta aaactatata
     2701 taaagttgat aataatggta actatattct agcaaaagag aaaaatggag ttcccctttc
     2761 agatgtttgg aatataccat ttcttaatcc aaaagctaaa gaaagagtag gttatcctac
     2821 acaaaaacct attctgttat tagaacaaat tataaagatt gctactgata aaaatgatat
     2881 agttttagac ccgttctgtg gaagtggaac tactttagta gcctccaaga ttttgaatag
     2941 aaattatatg gggattgatt tatctgagga agctatcaat ataactcagc aacgtctgga
     3001 aaatgttata aaaacaagtt caaatttatt gaataaagga atcgaagcat atagaaccaa
     3061 aactgaggaa gaggaaaaca ttcttaaatt attacaggca aaaattgttc aaagaaataa
     3121 aggaattgat ggttttttac ctaaacattt tcaaaaaaaa ccgataccta taaaaattca
     3181 aaaaaataat gaatgtctga atgagagtat ctctttatta cagaatgcta taaactccaa
     3241 aaaacttgat tttggagtag ttataaaaac tcattcagat aatttattat ttgattttga
     3301 tacgattcct gaaaatataa tagtagttga ccattttgaa cttacaatcg aaaaatggtt
     3361 aagtaaaagt caacagttac tatagtttgc aaattattta cagaaagaac aaaaaatgct
     3421 ctgaaacact gatttcaagg cattttttgt tagtttaaaa ttactcccat tcttcttttc
     3481 caaacgtaca atatatccaa aaccattcaa aatactagat tctatttttt ataatatcac
     3541 taaatccacc taattatagg acgttttcag atttttagtc ccgtcccagt cccagtaccg
     3601 gagaaatatt gttttaatat aatatctctt tttgtcttct aagctcttaa aagcaaaaga
     3661 acaagtaaag agtcaagaca aggataaaaa gtccatatta gggcaaataa aaagctttaa
     3721 gacagatgac aaatctaagt caaataagaa agaccatagc aaaggtgcag agagataaat
     3781 attggcggtc ttcggactgc ctttattttt ttatccattt ttcaaatcaa atttattcag
     3841 actatatatg cacatataca cttaaattca tataaaaaca tggcttgtaa aaaattactt
     3901 taatcacaat aatcgcattt aaaattgtga tgtttgcaag ctaaattacg gacttcactt
     3961 ggaagttttc ccttgtatct tttataatag atagaaaatt tgctggcaga tgaatatcca
     4021 acagattctg ctatctcttt tataggtagt tcagtgttta aaagaagagt ttcagctaca
     4081 ttcattcttt ttctttgagt gtactctgta atgctttgac aatatttttc cttaaataaa
     4141 ttttttaatt tagtaccact cattttagat attttttcaa gcgtgccttg atttacattc
     4201 gttgcaaaat gatcatctaa gaatcttgct acatcttcaa gtgctttatc atcatcaatt
     4261 tcaattttat atttttttct atttaagtat gtgtcaatta ctatacttat ccattcattt
     4321 gcctttgctt taaagaaaaa atcagcggca ggagcgtcca tcttacaatt taatatttcc
     4381 attgccactc tttctaaggc ctttgtaagt attatttgat tcggttgaag caaggttgaa
     4441 taaaaagatt ctggattaat gttaatagat gctaaatgtt tttctattag ctctttttta
     4501 aaaccaatgg aaacagcaag ataacaacaa ttctcgtgta ataaaaaaac aaaatcatct
     4561 tttatattat caaaatcaaa agtacataaa gagtttgcgg taatagtttg atacggatta
     4621 aacttttctc cgtttgcact gacaatgtaa cttgaataaa ttgaaacata gtctgacata
     4681 ctataagtgc tattttgaac tacttcctct ttgatataaa aatcatgtat atcgataatg
     4741 aagctgcctc cttcataaaa ccaatatagg ccttctgcat aagttgaatt ctcttttttc
     4801 caacaaaatg tatgacctgc acttgaatac ttctcattgt ttttacattc atctactttc
     4861 atataatctt ttacaaaatc ataatatgac ataacacact atccctttta gacaatattc
     4921 caattagcct tattaattca aaactattgt attagtaatt ataacagatg tataatagaa
     4981 aagcaatgat agatattatc aattaagcga atttatatct aaaagggata ttaaagaaag
     5041 gagatatgct tatgaagatt tacaaaaaac tatttgctta tgtccaagat aagaaatatc
     5101 ttggggtttt ggccataatt ttttctgcta tatctgctgt acttacagta tatggatatt
     5161 atttaatcta caaatttcta gataagttaa taattaattc aaacttatcc ggtgcagaga
     5221 gtatagcatt aaaatctgtt attacactaa caagtggagc gatattttat tttgtctcag
     5281 gaatgttttc acatatcttg ggatttaggc ttgaaacaaa tttaagaaaa aggggaatcg
     5341 atggtctgga aaaagcaagt tttaggttct ttgacttaaa tccatctggt caaataagaa
     5401 agatcataga tgataatgca gcacaaactc atcaggtggt agctcacatg attccagata
     5461 gctctcaggc aatagtcaca cctatacttg tccttgtact tggctttata gtaagtataa
     5521 gagttggcat aattttgctt gctcttacta taattggtgg cttaatttta ggggcaatga
     5581 tgggcgagca agaatttatg aagatatacc aagaatccct atctaaacta agtgctgaaa
     5641 ctgttgagta cgtgagagga atgcaagttg taaaaatatt taaagcaaat gtagagtctt
     5701 ttaaaagctt ttataaggcg ataaaagatt actcaaagta tgcttatgat tattccctat
     5761 cttgtaaaag gccttatgtt ttgtatcaat ggttattttt tggactgatt gcaattttaa
     5821 ttattcctat agtttatttt atgactagct tagctagcgc agaggtgatt ttacttgagc
     5881 ttatcatgat tttattttta tcaggagttc tctttgtttc attcatgaga atgatgtggt
     5941 actccatgta tatttctcaa ggaaattatg cagtagatac tttagaggcg ctttacgaag
     6001 atatgcaaaa agacaaatta gtgcatggta atgtcaataa ttttaaaaac tataatatag
     6061 aatttgagaa tgttagcttt gcttataatg ataaagctat cattgaaaat ttatccttta
     6121 atttagaaga aggaaagtcc tacgcacttg tcggttcatc tggatcaggc aaatcaacag
     6181 tagcaaaact tatatcaggt ttttacaatg ttaataaagg aagcataaag ataggcggga
     6241 tagcaataag tgaatattct gacgaagcct taattaaagc catttccttt gtttttcaag
     6301 attcaaaatt attcaagaag agcatttatg ataatgtagc tttagctaat aaagatgcga
     6361 cgaaagatga cgttatgaga gccttaaaat tagcaggatg cgatttaata ttagacaaat
     6421 tcccagaaag agaaaataca atcataggct caaaaggtgt ttatttatcc ggtggagaaa
     6481 aacaaagaat tgcaattgct agagcaattt taaaggattc caaaattatt attatggatg
     6541 aagcatcagc atctattgac ccagataacg agtttgaatt gcaaaaagct tttaaaaatc
     6601 ttatgaagga taaaacagtt atcatgattg cacacaggct atctacaatt aaagaccttg
     6661 atgaaattat tgtcatggat agtggaaaaa ttatagaaag agggtctgac aaagaattaa
     6721 tgtcaaaaga tacaaggtat aagagcctgc aagagatgtt taacagtgcg aatgaatgga
     6781 gggtttcaaa tgaaagagtt ttataaaaaa agatttgctc ttacagatgg aggagcaaga
     6841 aatttaagta aagcaacact ggcttcattt ttcgtttatt gtataaacat gcttcctgcc
     6901 atattactta tgatttttgc tcaggaagtt ttggaaaata tgggcaaaag caatggcttt
     6961 tatatagtat tctcagtttt gactttgata gcaatgtata ttttgctttc tatcgaatac
     7021 gataaattat ataacacaac ctatcaagaa agtgcagatt taagaataag gacagcggag
     7081 aatttatcaa aattacctct atcttacttt tctaaacatg acatttccga catttcacaa
     7141 acaatcatgg ctgatattga aggcatagag catgcaatga gccactcaat accaaaggtg
     7201 ggcggcatgg tactgttttt cccattaata tctgtaatga tgctagcggg caatgtcaag
     7261 atgggtttag ctgtaattat tccatctatt ttaagcttta tatttatacc tttatctaaa
     7321 aaatatcagg ttaatggaca gaatagatat tatgatgtct taagaaaaaa ctcagaaagc
     7381 tttcaagaaa atatcgaaat gcaaatggag attaaagcat ataatttatc gaaggatatt
     7441 aaagatgact tatataaaaa aatggaagat agtgagagaa tacacttaaa ggcggaagta
     7501 actacaattt taactttgtc tatatcttca atatttagct ttatatctct tgctgttgtg
     7561 atatttgtcg gcgtaaatct aattattaat aaagagataa attctctcta ccttatagga
     7621 tatttactag ctgctatgaa gataaaagac tctttagatg catctaaaga gggcttgatg
     7681 gaaatatttt atttatcgcc caaaatagaa agattaaaag aaattcaaaa tcaagattta
     7741 caagaaggcg atgactatag cttaaaaaaa tttgatattg atctaaaaga tgttgagttt
     7801 gcctacaata aagacgcaaa agttttaaat ggtgtaagtt ttaaagctaa gcagggagag
     7861 gtcactgctt tggtaggtgc aagtggctgc ggtaaaacaa ctatcttgaa acttatatca
     7921 agactttatg attatgacaa gggacaaatc ttaatcgatg gcaaagatat aaaagaaata
     7981 tcaacagaat ctctttttga taaggtctct atagttttcc aagatgtggt tctctttaat
     8041 caaagcgtta tggaaaatat tagaatcggt aagcaagatg caagtgacga agaggttaaa
     8101 agagcagcaa aacttgcaaa ttgcacagat tttatagaaa aaatggataa aggtttcgat
     8161 acagttattg gtgaaaacgg agctgagcta tcaggaggag aaagacaaag attatcaata
     8221 gccagagcct tcttaaaaga tgcgccgata ttgatcttag atgagataac agcaagcctt
     8281 gatgttaaca acgagaaaaa gattcaagag tctttaaata atttagttaa agataaaact
     8341 gttgtaatca tttcacatag aatgaaatcc atagaaaatg cagacaagat agtagttctt
     8401 caaaacggaa gagtagaaag cgaaggtaag catgaagagc ttttacaaaa atcaaaaatt
     8461 tacaaaaatt taatagaaaa gacaaaaatg gcagaagaat ttatttatta ggaggactac
     8521 aatggataat aaaaaattaa aagtaaaaga tttagtaagt atcggtgttt ttggcgtaat
     8581 ttattttgcc ttcatgtttg gagttggtat gatgggcttg attccaatat tgttcttaat
     8641 atacccgaca gtattagcca tagttgcagg aactgttgtt atgttattta tggctaaggt
     8701 tcaaaagcca tgggcactat ttatatttgg tatgatatca ccacttgtga tgtttgcagc
     8761 tggtcatacc tacgtagttg tggttttatc acttatagta atgataatag cagaattaat
     8821 tagaaagatt ggtaattata attcatttaa atacaatatg ctttcttatg caatcttcag
     8881 cacatggata tgtagctctt taatgcaaat gcttttagca aaagaaaaat atatggagtg
     8941 gtctttgatg acgatgggaa aagattatgt tgatgtatta gaaaagttaa taacttatcc
     9001 tcacatggct ttaatagcct taggtgcttt cttaggagga attcttggag catatatagg
     9061 caaggctcta ttgaaaaaac acttttcaaa tggattatat tgtgtgggat actttactcc
     9121 ttgcctaatt ttatggtgtt atctgaatta aaccctatag ttaagatgtt tttgagtata
     9181 cctattgtta ttagaatgtt tattttacca tttatggcag caagctttat gataaagacc
     9241 tcggatgtag gcgcaataat ttcatcgatg gataagctta agatttcaaa gaatgtatcc
     9301 atacctattg cggttatgtt tagattcttc ccatctttta aggaggagaa gaaaaacatc
     9361 aaaatggcta tgagagtaag agggataaat tttaaaaacc cagtcaaata tcttgaatat
     9421 gtttctgtgc cactactcat tatatcatct aatatatcag atgacattgc aaaagcggca
     9481 gaaacaaagg caatagaaaa tccaattgcc aagaccagat acattcgcgt aaagatacag
     9541 ctaattgatt ttgtttatgt tttagcggtt gctggactta ttgtgggagg cttaatatgg
     9601 ttgaaataaa aaatttaagt cttgattatg gtgaagagca tatattagat gatatatcac
     9661 tatccatagc cgagggagag tgcgtgctat ttacaggaaa aagtggaagt ggtaagtcat
     9721 ctttaataaa ttcaatcaat ggactagctg taaggtatga taacgcaaag acaaagggcg
     9781 aaataattat tgatggtaag aatataaaaa atttggaact ttatcaaatc tcaatgcttg
     9841 tttcaactgt ttttcaaaat cctaagacat atttttttaa tgtcaatacg acattagaat
     9901 tattatttta tttggaaaat atcggtcttg caagagaaga gatggacagg cgtttgaagg
     9961 atatacttga gatattcccg ataaaaaatc ttttgaacag aaatatattt aatctatccg
    10021 gcggtgaaaa acaaattctt tgcattgcag cttcttatat agcaggtaca aagattatag
    10081 ttatggatga gtcttcatcg aatttagata ttaaaagcat aagtgttttg gcaaagatgc
    10141 taaagatatt aaaagagaaa ggcataagca taattgttgc agagcataga atttattatt
    10201 tgatggacat agttgaccgt gtatttttaa tagataaagg aaagcttaaa aaaacttata
    10261 ctagaagtga atttttaaag ctagataaaa atgaattaaa tgctttaagt ttaagagata
    10321 aagaattaag taaattaaaa gttccttatt taaaagaagg tggagagtat cagataaaaa
    10381 atcttagcta caaatttact gatgatgagt gtttaagctt aaaagatatt tcgttcaagc
    10441 ttgggaaaat ttatggcata ataggatcca acggacgagg aaaatcaacg cttttaagat
    10501 gtttaatagg tcttgagaaa aaatcaaaag aagaaattta ttttaaggga gagaagctat
    10561 ctaaaaaaga aagactcaaa aactcttcac ttgttatgca agatgtaaat catcaattat
    10621 tcacagatga agtattcaac gagcttagat taggagtaaa gaattttgat gaagaaaagg
    10681 cgaaaatcat tttaaaagat ttaggcctgg acgaatttat tgaaaggcat ccgatgagtt
    10741 tatcaggagg gcaaaagcaa aggcttgcaa tagcatctgt tatgtgcaag acttctccat
    10801 ttatcttttt tgacgaacct tcaagtggta tagattattc caatatgata aaaatatctg
    10861 aactgattaa taagtataaa accatggata aaataatttt tattgtttcc catgatatag
    10921 aatttttaaa tgaagtggca gatgaaattt ttgaattgta aaatagagtc tgttaaacat
    10981 gtctatagaa ctggtaaatt gtctaaaaat ttaaaagata ctaacacata ttgatattta
    11041 ttagaaaatt aacttcagga tttttacagt atttataaac tttataatat gtatttatag
    11101 tattaataat ttccatatct ttagggaaat tatttattta cttaccataa ttaaagagat
    11161 gagagataaa tatcggcagt cttcggactg cctttatttt tttacttaat ttaatgcagt
    11221 aaatcatacg attgaaagga atctgcgtta tatgctataa ttaggtagtt aatctataca
    11281 tttagaaagg taaaatatag aattggagaa tgatatggga ttttcatata ataaattgta
    11341 accacccaat aacgaagtct attgaaaaat ctccagatta gtgaactcac ggatagttcc
    11401 taatctggag atttcttatt tgcacttttc ttgtacaact ttagtccatg gtaaataagc
    11461 ctctaaaacc tctttgttta cgagagtttc ctcgtttgga agacattcta gaagatagga
    11521 tagatatttc tcgctattta taatggattg aaataagata tgaacaaatc gattaggaaa
    11581 ggaaaattga tttatagaaa tattttagca gtcgatgcgt actgttatag attcaacgca
    11641 ctataattga tgacgtttag ctgtttccaa caaactcat
//
LOCUS       DAVBCB010000059        11318 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVBCB010000059 DAVBCB010000000
VERSION     DAVBCB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.059"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(7..1221)
                     /locus_tag="VQU28_001761"
     CDS             complement(7..1221)
                     /locus_tag="VQU28_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HET2477077.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(1377..1670)
                     /locus_tag="VQU28_001762"
     CDS             complement(1377..1670)
                     /locus_tag="VQU28_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477078.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVKVIMICQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            1832..2284
                     /locus_tag="VQU28_001763"
     CDS             1832..2284
                     /locus_tag="VQU28_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HET2477079.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(2319..3707)
                     /locus_tag="VQU28_001764"
     CDS             complement(2319..3707)
                     /locus_tag="VQU28_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HET2477080.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAAGKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            3773..4735
                     /locus_tag="VQU28_001765"
     CDS             3773..4735
                     /locus_tag="VQU28_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HET2477081.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(4732..4923)
                     /locus_tag="VQU28_001766"
     CDS             complement(4732..4923)
                     /locus_tag="VQU28_001766"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477082.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDETDEVSSKHVFEVVDRTDEVS
                     NHTYGRATLTWFAEIFEEY"
     gene            complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VQU28_001767"
     CDS             complement(4987..5997)
                     /gene="ccpA"
                     /locus_tag="VQU28_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HET2477083.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKVGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(6422..7021)
                     /locus_tag="VQU28_001768"
     CDS             complement(6422..7021)
                     /locus_tag="VQU28_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2477084.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(7022..8119)
                     /locus_tag="VQU28_001769"
     CDS             complement(7022..8119)
                     /locus_tag="VQU28_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HET2477085.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(8119..8856)
                     /locus_tag="VQU28_001770"
     CDS             complement(8119..8856)
                     /locus_tag="VQU28_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2477086.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(8858..9742)
                     /locus_tag="VQU28_001771"
     CDS             complement(8858..9742)
                     /locus_tag="VQU28_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477087.1"
                     /translation="MNMIKVQGLHKNIKSKAILKDISFEVAEGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(9729..9923)
                     /locus_tag="VQU28_001772"
     CDS             complement(9729..9923)
                     /locus_tag="VQU28_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477088.1"
                     /translation="MKKAILFLFIGLSLVVWLVEMFTGWFDQSFLHQVIRGAWGLGFM
                     ISVVFPMGMEWLKGEYYEHD"
     gene            complement(9920..10126)
                     /locus_tag="VQU28_001773"
     CDS             complement(9920..10126)
                     /locus_tag="VQU28_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477089.1"
                     /translation="MEEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEGFYHFPWELFAS
                     FGIMSWLIREGLKLVRDVKKELEK"
     gene            complement(10611..10684)
                     /locus_tag="VQU28_001774"
     tRNA            complement(10611..10684)
                     /locus_tag="VQU28_001774"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10648..10650),aa:Pro,seq:tgg)
     gene            complement(10700..10773)
                     /locus_tag="VQU28_001775"
     tRNA            complement(10700..10773)
                     /locus_tag="VQU28_001775"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10737..10739),aa:Arg,seq:acg)
     gene            complement(10783..10868)
                     /locus_tag="VQU28_001776"
     tRNA            complement(10783..10868)
                     /locus_tag="VQU28_001776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10832..10834),aa:Leu,seq:taa)
     gene            complement(10876..10947)
                     /locus_tag="VQU28_001777"
     tRNA            complement(10876..10947)
                     /locus_tag="VQU28_001777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10913..10915),aa:Gly,seq:gcc)
     gene            complement(10970..11042)
                     /locus_tag="VQU28_001778"
     tRNA            complement(10970..11042)
                     /locus_tag="VQU28_001778"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11007..11009),aa:Thr,seq:tgt)
     gene            complement(11061..11142)
                     /locus_tag="VQU28_001779"
     tRNA            complement(11061..11142)
                     /locus_tag="VQU28_001779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11106..11108),aa:Leu,seq:tag)
     gene            complement(11148..11220)
                     /locus_tag="VQU28_001780"
     tRNA            complement(11148..11220)
                     /locus_tag="VQU28_001780"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11185..11187),aa:Lys,seq:ttt)
     gene            complement(11245..11317)
                     /locus_tag="VQU28_001781"
     tRNA            complement(11245..11317)
                     /locus_tag="VQU28_001781"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11282..11284),aa:Asp,seq:gtc)
BASE COUNT         3401 a         2619 c         2087 g         3211 t
ORIGIN      
        1 caagccctat ctacgatact gtttcaagaa acgagtcatc ttttcttgga tttgggctaa
       61 ctcatcaaca cgaggaaggt aaacgatacg gaagtggtct ggttcctgcc agttaaagcc
      121 tcgaccatga accaagagaa ccttttcctg cttcaagaaa tcaaggacaa actgctcatc
      181 atcatcgata cggtacatat tgcggtcgat ttttgggaag atatagagcc ccgccttggg
      241 tttaacggca gacaaacctg gaatatcttg aatggcatta tagatgaaat ttctttgctc
      301 gtagattcgt ccaccaggaa gaagcaattc atcgactgat tggtgacccc ccaaggaagt
      361 ttgtacgact tgttgggcca aaacgttaga gcaaaggcgc atattggaca gcatattgag
      421 cccttcgata tagcccttaa catgagtctt agggccagac aagaccatcc aacccacacg
      481 gaaacctgct atgcggtggg attttgacag accattcatg ctgacacaga agacatctgg
      541 tgccaagctc gccacaggcg tatgcacatg tccgtccatt accatgcggt catagatttc
      601 atccgcaaag ataatcaaat cgttttgacg ggcaatctca ataatctcca gcaagagttc
      661 cttaggataa agggctccag ttgggttatt tggattgata aggacaattg ccttggtatt
      721 ggaagtgatt tttgacttga tatcgtcaat atctgggtac cattccacag cttcatcaca
      781 gatatagtga acggcatttc ctccagctag gctgacagcc gccgtccata gaggatagtc
      841 tggcattggc actaagacct catccccatt gtccaaaagc ccctgcatgg acatgacaat
      901 caactcactg acaccatttc caaggtaaat atcatcaata tctacgtttg ggaatttctt
      961 cagctggcaa tactgcatga tggccttacg ggctgagaaa atccctttgg agtcagaata
     1021 gccttcacta tcacgcgcat tcataatcaa gtcatgaatg acctcgtctg gcgctgtaaa
     1081 gccaaattct gctggatttc ctgtattcag acgtaaaatc ttttctccat tcgctcgcat
     1141 ccgcatggct tcttccaata caggaccacg gatatcataa gcaacatgct ctaacttact
     1201 agacttgtta tattctttca tcttgtttcc tcaattcatc aggatagtaa cattatacca
     1261 ctagaaagag aatgcgacaa gtttactgaa atgtgttact agtttcgtat agaagaaaaa
     1321 ccttgctaaa ggcgaggtta agaacgtaga gatagcaggc attttgcaaa gacacatcac
     1381 ataccaaaag ataaacctag aaaaagcaag cctagaaagg agtcataaag aagattaaac
     1441 atgaggaaaa gcccccatat catcaagtaa tcccaacgcc cacttcgttt tgcaactaga
     1501 aataagatta gatggtagag tacatgaaag actaaaaatc caataaaact tggataaaga
     1561 agcggtacca gactctctaa ttgacagatc atgatcactt ttaccaagac tgaaactagg
     1621 ataattccat aatacaggaa atttgatttt tgaaaagagt ttttcatcat tctcccttcc
     1681 ttcacttctt tccttctatc ctttctttta ttttatcata tatttatata gattttaagc
     1741 cctaatattc ttttgaaaaa tacttttttt gctttacttc tatagtgaaa aggtttacaa
     1801 ttatagtaag aaaatttcag gaggtttcat tatggcacaa cgttaccaaa atatcatggt
     1861 cgcaatcgat ggttctaagg aagcggactt ggcttttgtc aagggagttc attctgctct
     1921 acgaaacgac gctaaactca ccatcgcaca tgtcattgac acacgcgctc tccaaagcgt
     1981 atccaccttt gatgctgaag tttacgaaga actccaagtc gacgctgaaa gtctgatgaa
     2041 agagtacgaa aaacgtgcta aagatgctgg ggtagcagat gttcatatcg tcattgaaat
     2101 gggaaatcca aagaccctgc tagcacgtac tattccagat gccgaggaag tggacctcat
     2161 cctcgttggc gcaactggtc tcaacgcctt tgaacgcctc ttggtcggct cttcatctga
     2221 atacatactc cgccatgcta aggtcgattt gctggttgtg agagaacaag aaaaaacctt
     2281 ataatcacaa agaaaaggag cccctagctc ctttttgttt acgatttatt tctctcttta
     2341 tggcgttcgt aagccttgag ctggcgctgc agttcctttt taatagcagg ttctggagca
     2401 tatttttctt cccaattatc tggttttaag attttatggg tcactggatc aaaatgagcc
     2461 ttgccatctg gaaaaatttt ccccatattg gcctgatgga caatatcaaa aatacgttct
     2521 gggtccaccc ccatcaagac aaaactgccg taggtgaagt aaagcgtgtc aatcaaggca
     2581 tccacttgcc ctatcaaatc ttgctgagca ggtgtcttct tggctacttt acctgctgcc
     2641 ttatcaaggg cctgatgaag ttgcgataca gcttgaccaa aatcttcttc agaaggactg
     2701 gctgctcgaa caaactccac caattcttct attttaaaac cagccctatg ggttgcaccc
     2761 tctaaatccc aagctcgagg ttcttcttgg gttcgttcat ccatcatgtg gtggaaggtc
     2821 ttgaccttat tgaaatgata gtcacggctg acaaagactt tttctgaaga caaaacacca
     2881 aaatgttcca aggccttgtg gatcccatct tgttgattgc tagtggtaat gtgcttggca
     2941 gcttccttga cactgctact accatttccc atagcgaccg acataccgac gccagccagc
     3001 atttcaaggt cattatctga gtcaccaaaa gccatgactt ggttgaggtc aaagccatat
     3061 tctttcccaa ctcggcgaat gccttctaat ttagaatttc cctgattgat gacatccgat
     3121 gcaaaaggat tgctacgtgt caatttcaag tcttcaaaat cagctgccgc cttctcagat
     3181 tcttctggtg tcatcagcat caaaacttgg tagataggct gattcatcag gtgaagcagg
     3241 tcctcttcct tttggggaac aaccttgctg accatgcgat taaaagactg actcaccgtc
     3301 cgagttaaaa cagagggaac gaagcgacta attcgttggg aaaaagaacc cagaccaaag
     3361 gacatgattt tagaacccaa catggcatcc ttggtcccta gggcaatctc cgtgccctct
     3421 tttttagcat agctaattag atggcgcaaa tgtaacttgg aaatagggct cgtgaacaag
     3481 actctgtctt tactaaagat atactggcca ttataggtta ccgcaaaatc cagatccaaa
     3541 tcgtccatca attccttaac aaaaaaaggt cctcgccctg tcgctacgcc aactagtacc
     3601 ccttgttctt tgacaatctt aatcgcgtcc ttagtggatt tcaaaacact cttgcgattg
     3661 ttgaccaagg ttccatcgat atcaaaaaaa acagctttga cttccatcct attccaatct
     3721 ccccttttgt gatacaatga ttataccaca tttcagaaag agtgagtaaa tcatgcctaa
     3781 gaaaatcctt gttttacata cgggtggaac tatttccatg caggccgatg cttctggcgc
     3841 tgttgtgacg agttcagata atcccatgaa ccatgtgtcc aacccacttg aaggaatcca
     3901 agtccacgcc ttggactttt ttaaccttcc aagtccccat atcaaaccca aacatatgct
     3961 ggtcctctac cagaaaatta aagaggaagc agataactac gatggagtgg taatcacaca
     4021 cggaaccgat actttagagg aaacagccta tttccttgat accatggaag ttccccatat
     4081 gcctatcgtt ctaacaggag ccatgcgtag ctccaatgag ctcggtagtg atggtgttta
     4141 taattaccta agtgctttac gagtggccag cgatgacagg gctgctgaca aaggagtttt
     4201 ggtcgttatg aacgatgaaa tccacgctgc caagtatgtc accaaaacac atacgactaa
     4261 tgtcagcacc ttccagactc caacacatgg ccccctcggt ctcatcatga aacaggaaat
     4321 cctctacttc aaaacagctg aacctcgtgt tcgctttgac cttgatcaca tacaaggttt
     4381 agtccctatc atctcggctt atgctggtat gacagatgag ctgattgata tgctggattt
     4441 agaacacttg gacggtttga ttatccaagc cttcggagct ggtaatattc ccaaagaaac
     4501 ggctcaaaaa ttagaaagcc ttctgcaaaa aggaatccca gtcgctctgg tatcacgatg
     4561 ctttaacggt attgccgagc ctgtttatgc ctaccagggt gggggcgtac agttgcaaaa
     4621 agcaggcgtt ttctttgtta aagaactcaa cgcccaaaaa gctcgcttga aactcctcat
     4681 cgccctcaat gccggactaa caggacaggc tttgaaagac tatatggaag gctaatactc
     4741 ttcgaaaatc tctgcaaacc acgtcagagt cgccctaccg tatgtatggt tactgacttc
     4801 gtcagttcta tctacaacct caaaaacatg ttttgagctg acttcgtcag tttcatctac
     4861 aacctcaaaa cagtgtgttg cgcagccttt aaccagccgc ctagtccgct ctatggtatt
     4921 cattaagtca acatctcttg tttaagagca caaaatcagg aaatcttctc gattccctga
     4981 ttttttctat ttacgttttc gtgttgagct acgttctgtc aaaccatgag gtaagagaac
     5041 ttcacgttct tccaactctt ccttatgcat aatcttggtc aacatacgca tactaatggc
     5101 accaaggtca taaagaggtt gggcaatcgt tgtcaagttt ggacgggtaa agcgtgagat
     5161 ttgtgaatca tcactagtaa taatttcaaa atcttctggc acagaaacac ccttatcagc
     5221 caaaccgttc aagactcctg ctgccaactc atcacctgtc acaactgctg cagttgcatt
     5281 tgatgaaatc aaacgctctg ctaaggcgta accatcatca tagctatatt tagattcaaa
     5341 taccaaaccc tcactataag tgattcctac ttttttcaag gtttccttgt agccaactaa
     5401 acgaacctta ccattgatgt catccactag cggaccgcta acgaaagcaa tacgctcatt
     5461 ttctttagca aggtagctca ctgcatcaat tgttgcttgc ttatagtcaa tattgacact
     5521 tggcaactgg tgctcaacat cgacagttcc tgcgagaaca atcggagtac gtgaacgcga
     5581 aaattctgag cgaattttat ctgtcaagtg ataacccata tagataatgc catctacctg
     5641 ctttgaaaag agggtattga caacagaaac ttctttctcg ttatcttcat cgctattagc
     5701 taggacaata ttgtacttgt acatttctgc aatatcatca atccccttag ccaaactcga
     5761 aaaataacca ttggtaatat ttggaatcac gacaccgaca gtggttgtct ttttacttgc
     5821 aagaccacgc gcaactgcat ttggacgata atccaaacga tcaattacct ctagcacttt
     5881 tttacgggta ttctctttta catttttatt gccattgacc acacggctga ccgtcgccat
     5941 ggaaacacct gcttcacgag cgacatcata aatggttact gtatcatctg cattcattcc
     6001 ttttcctgtc ctttctatct cacacattct tttacaagta gaggtactga ttgaagctct
     6061 atatctactt acaaaagtga agatgtgaaa atttcgtttt catatttcta cttattccat
     6121 tctatcacta attgtaaaca ctttcaagtg ttttttgaag attgattgaa aaaatttcat
     6181 agaaaaccta ggtttagctc cttgctacca ccttagacta aataaaaagg aggaaactaa
     6241 gcccttctaa agttatagta aaatgaaata agaacaggat aaatcgatca ggacagtcaa
     6301 atcgatttct aacaatgttt tagaagtaga ggtgtactat tctagtttca atctactata
     6361 ggtattgttc cattcactac cgtcaatttt agcacatagt cttcatgaaa atattatatc
     6421 atcataacca accagattct tttgcgatat tagctgcctc tgttcgatta ccagcatcca
     6481 gttttgaaag aatattggtg acatagtttc gaatagttcc gtttgagagg taaagctgat
     6541 ctgcgatttc ttggttggat aagccccggg cgattccctt taagacagca atctcttgtt
     6601 ctgtcaacgg attgggacgg gtcatcacca cttccatcaa ttcaggcgaa tactccttgc
     6661 gtccttcgag gacggtgtgc aaggtttgca tgaggtctgc aatgcttcgt tcctttaata
     6721 cataagcatc cactccagcc ttgaccgcac gttcaaaata cccagcacgc ttgaaggtcg
     6781 tcaccacaac cacctttgtt tcaagccttt ctgatcgtat ccactccaag acttcaagac
     6841 ctgtcttaac aggcatttct acgtcaagga tggcgatatc tacagactcc ttttctaata
     6901 gttggattgc ttcttgccca ttcttggctt gaaggacaga ctccacatct ggttgaagcg
     6961 tgagcaattg acacatagca tctcgcaaca tactttgatc ttctgcgact aatactttca
     7021 tctactttct ctccttataa ggtagtcgaa cttgcacttc cgttggatgt ttctgactga
     7081 ttacacttac ttctcctgaa aatggaagga cacgttctcg aactgtatgg agctcatttt
     7141 ctgttacagt ttcaaaacct ttcccgtcat ctctgaccat caggagcaat tcctgatctg
     7201 tgcgttctaa ttttaagtag gctttagacg ctttagcatg tttgatgatg ttggtcacta
     7261 attcaagcaa aatcatggaa gccgttgact ctaattcctg agttaagcta gctgtatcta
     7321 gttgattagc tatttccacc tcaattccag caatttctaa catctttttc acagtctcta
     7381 gttcggatgt caaagttcta gacttaagat tttccacaat ggttcgcact tcacgcattg
     7441 attctttgct aatctgctgt atttctctta attccttttc cacttgtgga taagcctgta
     7501 tttgaaataa ctgcaaggct agatcagttt taacactaag catagcaaag gtatgcccca
     7561 gactatcatg caaatcttga ccgatacgat tgcgctcatt ttctgctagc aatagattta
     7621 tctgggtatt ttgcttagtc tgagcttctt tcaaatcctc gacaaggcga attcgaaccg
     7681 agcccaaagt catcaaatca acaaaagtaa gaattccaag cagatagact agaaactcaa
     7741 cttcgattct ctgaaaaatc aaaagtcgcc ccacaacaag gacttgagca agaagaaaag
     7801 tccagacatg taaagacttt aaactacgta cgctgaaatg ataacttaag agattggaaa
     7861 ggaaaaagaa aaaccagata taattcacag caacaaaggc ggtattccca gctacataga
     7921 tcagcatgat cccccagaaa agccaagata ggcgctggct cttagttgtt aaaacaccca
     7981 aatatgccac tacaaataga atatcaatca ataaatgcca ggcagaaagc cacccagtca
     8041 ctacaggtag gatggggaaa atcataaaaa ttaaactggc ccaaaacata tagtgtatgc
     8101 ttttcagtct ttcaagcatt aagcattctc cttatgacct tgaaggtaaa tggtcaaacc
     8161 aaacaaaact gctgaaaaaa caagtagata aactgtggct gatagattga tgctaccctc
     8221 gtttaagaag gtcttgagca actccatcaa ctgataagtt ggtaggcact tcccgattgc
     8281 ttgcatccag tctggaaata aagagatggg catccagagt ccacctaaaa cagccaagcc
     8341 tagatagaga agattgccca cgacagacat cagctgacta gaaggcaaga gtgtcaaggt
     8401 caagccaagc gctacaaaag ctatacttcc tactatcagc aagaacgcag ccccaatcca
     8461 gcttcctaaa gacatatcca cacctctgac caagtgccca actgagaaaa caaccaggat
     8521 tgagaccaaa taatcaacca tcatacttgt tatctttgat agataatatt ctaccatatt
     8581 tacagggcta tgacgcaatg ttttctgcca gttgttcatc ttatcggtat gtaagacagc
     8641 tgggaatgaa aagatagcgg tcgacatcat ggaaaaggca gtcatggaga tgaggtagtc
     8701 gcgcataaaa ttagccggtc cacctggtgt gtcctggtac atgcctgaaa agaatagata
     8761 gaaggctgtc ggcatcccta cagataataa ataatagact gattgccgtt tggtcaatag
     8821 aaattctatc ttgtttagtg caatccatcg tttcatctta gtcatctccc ttctgtgttt
     8881 cttcaaagat tgtatccaac aaactacgat tattgacttc gatttcttgt atcctacatc
     8941 ctgcttgaac taacagttcc cagaaagcat ccgcttcgca tgtgactact tgtaaggcat
     9001 cttgtttttg tacccagttt tcaaccaaga ctgacttctc aacgacttcc ttgtaagcta
     9061 gcggaaggat aaaatgcttt tcaatctcct cactacgcat agctagaggc gtcgtgtcac
     9121 gaatcaactc tcccttattt aaaaccaaaa tccggtcagc tgtatgctct acctcttcaa
     9181 tataatgaga cgaatagaga atggtgactc cttgcgcttt taggtcccga acaatttccc
     9241 aaaaacgttg acgagttgaa gtatccatgg cagcagttgg ctcatctaaa aagacaagct
     9301 ttggtcgccc aattaaggtc aagataaaag ataaaagacg cttttgccca cctgacaatt
     9361 tttctgcgaa ttgctctttc tgttgctggt caaactgtaa tagttgatcg atttcctggt
     9421 tgttcaaggg atttggatag atacgttgaa agaaagcaat caactcttta acctttagtt
     9481 tttgaacaat tacattttct tgagggagat aggatctcgt atagtctaac ttagagctcg
     9541 ttaccggcaa atcttggata tatacttgcc cactggtgac caatttatct ccaagcaaac
     9601 agtccaagag tgtcgtctta ccagctccat ttggcccaat caaggcgacg cattcacctt
     9661 cagctacctc aaaggaaata tccttcaaaa tagccttact tttgatgttt ttatgtaagc
     9721 cttgtacctt aatcatgttc ataatattct cctttcaacc actccattcc catagggaaa
     9781 acgacggaaa tcataaatcc taatccccag gcgccgcgaa taacttggtg aagaaaggat
     9841 tgatcaaacc aacctgtaaa catttctacc aaccatacca ctagtgatag tccaataaaa
     9901 agaaagagaa tcgctttttt catttttcaa gctccttttt cacatctctt actaatttca
     9961 agccttctcg tatcaaccaa gacatgattc caaagcttgc aaacaactcc caaggaaaat
    10021 gatagaatcc ttcgtccaat cctgagaaca tgagataagt catgactcct gctactacta
    10081 aactcactgc gataatcatt ttatttctca tctcttcttc ctccatttca tactacaatt
    10141 atagtctttt gaaatcagag gagatagaag cttctgtcac taaaaaatat gacaaatgtc
    10201 ataaaaaatt ctgttcaaaa caagcaagat acactataca ataaaacaca attagataaa
    10261 tctaaggcaa cttcctcaaa agagatatca aaccccattc acactataat gtaaactaat
    10321 acttatttaa agtcaaaaga gcggaaattt ttatcgtaca aacatatata ttataggtaa
    10381 gacaaaaaac tcttcaatta ctttcttaaa aatttatagg agttataaaa tctattaaat
    10441 ttcaatgtgg atacctaaac tacagtattt attgaaaaga ctggagacaa aaagtatacg
    10501 ctgccaaaat gaattgctga aaatcaaaaa agagagaagc aaactgattc tctcttaatg
    10561 tatataatat ctagttataa aaatacacac tcacatatct ctgtaatgaa tcgggaagac
    10621 aggattcgaa cctgcgacac cttggtccca aaccaagtac tctaccaagc tgagctactt
    10681 cccgagttaa atagaaaaat gcaccctaga ggagtcgaac ctctaaccgc ctgattcgta
    10741 gtcaggtact ctatccagtt gagctaaggg tgctccatat tatgccgagg accggaatcg
    10801 aaccggtacg atcgttacca atcgcaggat tttaagtcct gtgcgtctgc cagttccgcc
    10861 accccggcct ctctaagcga acgacgggat tcgaacccgc gacccccacc ttggcaaggt
    10921 ggtgttctac cactgaacta cgttcgcact gttttcttct atctaaaaat gccggctaca
    10981 tgacttgaac acgcgaccct ctgattacaa atcagatgct ctaccaactg agctaagccg
    11041 gctcatttgt tatatcttaa tgcgggttaa gggacttgaa cccccacgcc gttaagcgcc
    11101 agatcctaaa tctggtgcgt ctgccaattc cgccaaaccc gcatatatga cccgtactgg
    11161 gctcgaacca gtgacccatt gattaaaagt caattgctct accaactgag ctaacgagtc
    11221 taaaataact tgcgttacct taaacggtcc cgacgggaat cgaacccgcg atcttcgccg
    11281 tgacagggcg acgtgataac cgctacacta cgggacct
//
LOCUS       DAVBCB010000060        11303 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVBCB010000060 DAVBCB010000000
VERSION     DAVBCB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11303
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.060"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..738
                     /locus_tag="VQU28_001782"
                     /pseudo
     CDS             1..738
                     /locus_tag="VQU28_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(854..1657)
                     /locus_tag="VQU28_001783"
     CDS             complement(854..1657)
                     /locus_tag="VQU28_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HET2477090.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            complement(1936..2691)
                     /locus_tag="VQU28_001784"
     CDS             complement(1936..2691)
                     /locus_tag="VQU28_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HET2477091.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
     gene            complement(2761..5412)
                     /locus_tag="VQU28_001785"
     CDS             complement(2761..5412)
                     /locus_tag="VQU28_001785"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HET2477092.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            complement(5405..5983)
                     /locus_tag="VQU28_001786"
     CDS             complement(5405..5983)
                     /locus_tag="VQU28_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HET2477093.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            complement(5983..6453)
                     /locus_tag="VQU28_001787"
     CDS             complement(5983..6453)
                     /locus_tag="VQU28_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2477094.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            complement(6440..7018)
                     /locus_tag="VQU28_001788"
     CDS             complement(6440..7018)
                     /locus_tag="VQU28_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HET2477095.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            complement(7301..7696)
                     /locus_tag="VQU28_001789"
     CDS             complement(7301..7696)
                     /locus_tag="VQU28_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2477096.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            complement(7792..8046)
                     /locus_tag="VQU28_001790"
     CDS             complement(7792..8046)
                     /locus_tag="VQU28_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HET2477097.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            complement(8055..9389)
                     /locus_tag="VQU28_001791"
     CDS             complement(8055..9389)
                     /locus_tag="VQU28_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HET2477098.1"
                     /translation="MTDEWIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVLDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGSKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            complement(9389..9622)
                     /locus_tag="VQU28_001792"
     CDS             complement(9389..9622)
                     /locus_tag="VQU28_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HET2477099.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLTGTPMDKIVRTLEANGYEVIGLD"
     gene            9653..9727
                     /locus_tag="VQU28_001793"
                     /pseudo
     CDS             9653..9727
                     /locus_tag="VQU28_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            9716..9841
                     /locus_tag="VQU28_001794"
     CDS             9716..9841
                     /locus_tag="VQU28_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysyl-tRNA synthetase"
                     /protein_id="HET2477100.1"
                     /translation="MDRTDEVSNHTYSKVKLTWFEEIFEEYKMILLLILYHMERD"
     gene            complement(9891..10031)
                     /locus_tag="VQU28_001795"
     CDS             complement(9891..10031)
                     /locus_tag="VQU28_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477101.1"
                     /translation="MKFFWTILAILFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(10028..10210)
                     /locus_tag="VQU28_001796"
     CDS             complement(10028..10210)
                     /locus_tag="VQU28_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477102.1"
                     /translation="MNPIKAFAKIYGNYFLTVQGVKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(10212..10995)
                     /locus_tag="VQU28_001797"
                     /pseudo
     CDS             complement(10212..10995)
                     /locus_tag="VQU28_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3333 a         2475 c         2093 g         3402 t
ORIGIN      
        1 aaaacagata aggaccaaga gcttaaagct gaaattcaat ccatttttat cgaacacaag
       61 ggaaattatg cttatcgctg tgttcattta gaactaagaa atcgtggtta tctggtaaat
      121 cataaaagag ttcaaggctt gatgaaagta ctcaatttac aagctaaaat gcgacagaaa
      181 cgaaaataaa ggagacgttg gtaagaaggc agagaatctc attcaaggcc aatttgaagg
      241 atctaaaaca atggaaaagt gctacacaga tgtgactgaa tttgccattc cagcaagtac
      301 tcaaaagctt tacttatcac cagttttaga tggctttaac agcgaaatta tcgcctataa
      361 tctttcaact tcacccaact tagaacaagt acaaacaatg ttggaacagg cattcacaga
      421 gaagcactac gagaatacga ttctccatag tgaccaaggc tggcaatacc gacacgattc
      481 ttatcatcgg ttcctaagag taagggaatt caagcatcta tgtcacgcaa gggaaacagc
      541 caagacaacg gtatgatgga gtccttcttt gggattctga aatcggaaat gttttacggt
      601 tatgagaagt cgtttcagtc gcttaagcaa ttggaacaag ccattataga ctatattgat
      661 tactacaaca ataaacgaat taaggtaaaa ctaaaaggac ttagccttgt gcaatacaga
      721 actaaatcct tcggataaat tatttgtcta actttttggg gtcagtacac ctaaaacttt
      781 gatgatatac gttttcttgt gagaatattt acttcatttt tgcctaaaat tcaatgttta
      841 ctcagtattt ggattatgaa aaatcgaggt ctaaatctag atacattttt tctgaagaca
      901 aatcattttg accaccgagc aagagatttt caaaaaaagc tgttaaaaac tcagaacgtc
      961 gctgtaaaat ctttgcatta tctaatacca aggcatcacg aaaatatttg gaatgttgct
     1021 gaaatggtgt attatcaata tcaaaaccaa actcacgaag atactgaatc aaaaagaccg
     1081 ttactgtccg agtgtttcct tcgcgaaatg gatgaatctg ccagattcct gaaataaaat
     1141 gctggatttg tttaaccaca tccgcctgag ttagtgtcgc atatgcaact tgtttttcct
     1201 gattaaaatc ataatctaag gtcatttgaa tcatggagta atcagagtac acaacacttt
     1261 caccattcaa aacaggttca ttctttgtga tattggtctg acgaaattgc cccaccggaa
     1321 tagagggttc aaatatatct tgaaacaact ccttatgaat agcaagtaag gtcgcaggac
     1381 taaagctaaa gcctcttcga gacaatagtt ctacaatacg tagagaaacc aagtctgcct
     1441 cttccgtact tgcatcaata gtatggtgat aagccgttgc atcctcataa acctgctcat
     1501 aagtcagttc tccccgggac tgtttctcag ccaaagattc catatacgct gatggcacta
     1561 gattgtcaac tttctgcaga ccaaaaccta tccgccataa atcacgcttc gcttcataag
     1621 acaagtttgg attgtcaatg ttgtaagttg attgcataga aatatccttt caattgtttt
     1681 tcttcattac cagtaaatca cgaaattggt tggaaactgg cttaaactat aaaaagtcct
     1741 cacaataggt tctatgatat atatatatat acttcatgta aataataaaa gagttataat
     1801 cagcactact gataaattca ataccgattt cgccatctta aaacttagtc aaacgaatac
     1861 cgttgatttc cagttcatta agtcgttgac ctgtcaagac ttctcgttcc ttcaactgga
     1921 aaagttaagg gggaactaat acaaatcctc tacgcttcta tattcaatga tttcattttt
     1981 gataatattc tttttaaact caaaatgttt taaaggatta tcgattaaca ctaactttgg
     2041 aatatcatct agatttgtga ccaaggataa aataaaatcc gattgaaact ccttgacttt
     2101 ttcaaattct tttgcagtta aacgcacacg ccccttagat tttcggatac cttttacttc
     2161 ggcaaggtaa gaatactctt ggacatctac ctgaaagtca tatccatcac cataaagacg
     2221 ggcatcggtt aactgtccac cttggaattt ttcttcttta tcaaaatgaa ggataaaata
     2281 attctcagcc tccattcccg tttcctgtag acgtttgaat ttagttcgaa ctattggttt
     2341 ttcaacgatg gttatgcctg ttttcttccc ttcactggca agtaacattt taactatttc
     2401 agcataacta tgaacatctt catttccaaa catcatatca attaaatctt tacgaaaacg
     2461 ataaacttca gctttctgcc accaaccttt tcgattattg tcaaaataag gatcaaataa
     2521 atccattcta ttttttacaa ctccagttgt ttcagcaatt cccagagaaa ccacatagcg
     2581 ataaaactcc gacttgctag agcattgaaa ttcttttata aaaagcttgt caaacttggc
     2641 aagaccgtag ccaattaaat ttaacaattc ataatgaggg tgtttggaca tcactttctc
     2701 catcaataga tattgatttc attataccaa aaaacacggc tcatcaccgt gtttctgtgt
     2761 ttatttcacc aacttcttca tctcatcaat acgtgctacg gtcgcatcat acttagcttg
     2821 gtaatctgct tgcttgtcgc gttctttttg gacaacttct ggtttagcgt tggcgacgaa
     2881 gcgttcgttg ctgagttttt tgccaaccat gtcgagttct ttctgccatt tggcaagttc
     2941 tttttccaga cgtgccaatt cttcttcgac attcaagagg tctaccagtg gcaagaagat
     3001 ttcggctcct gtgatgacgc ttgacatagc cagttcaggt gcagggatgg ttgatgcgat
     3061 ttccaagtgt tcaggatttg tgaaacgttt gatgtagttg acattgctgt taaagaaggc
     3121 ttccaagtcg ctatcgcttg tcttaacaag gatagtgata ggcttgcttg gagcaacgtt
     3181 tacttccgca cgcgcattac gaacggcacg gatcaagtct ttaaggcttt ccacaccagt
     3241 gtgagcagca aggtcttcaa aggctgggtt gacagttggg tattctgctg taacgataga
     3301 tccttctgag atttgtccaa agatttcctc tgtcacaaat ggcatgattg ggtgaaggag
     3361 acgaaggatc ttgtccaaag tgtatagtag aacagaacgt gtgatgactt tctcttcttc
     3421 gttatcgcta taaaggactt ccttggtcaa ctcaacatac cagttggcaa attcttccca
     3481 gatgaagttg taaaggatat gaccagccac accaaactcg aacttatcaa agttttcagt
     3541 aacttttgca atggtttcgt tgagattgtg gagaatccag cggtccgtca cattaccagc
     3601 ctcacctgtt gcaacttttg tgacattgtc atgcgccaca tccagcgtca aaccttcatt
     3661 gttcatgagg atatagcgag aaatgttcca aattttgtta ataaagttcc atgaagcatc
     3721 cattttctcg taagagaaac gaacgtcttg accaggagct gagccgtttg acaagaacca
     3781 acgaagacta tctgtaccat atttatcaat aacatccatt gggtcaatgc catttccaag
     3841 agatttagac atcttacgtc cttcttcatc acgaattaga ccatggataa gagcattttt
     3901 aaatgggctc tttcctgtaa attcaattcc ttggaaaatc atgcgtgata cccagaaagg
     3961 aataatatca tagcctgtta cgagtgttga atttggatag tagcgtttaa agtcagtagt
     4021 ttcggtatta ggccagccca tggttgagaa gggccagagg gcagagctga accaagtgtc
     4081 aaggacatct tcgtcctgtt tccagccatc gcccgctggg gcttcctcgc cgacatagat
     4141 ttcgcctgct tcattatacc aagcagggat ttggtgaccc caccagagct gacgggaaat
     4201 cacccagtcg tggacatttt ccatccattg caggaaggtg tcgttgaagc gcggtgggta
     4261 aaattccacc ttgtcatctg tgtcttggtt ggcaatggcg ttcttggcca attggtccat
     4321 cttgacgaac cattgagtag acaagcgtgg ctcaactacg acacctgtcc gttctgagtg
     4381 accgactgaa tggacacgtt tttcgatttt gacaagggca ccgatttctt ccaacttagc
     4441 aacgactgcc ttacgagctt caaaacgatc catgcctgaa aattcaaagg caagctcgtt
     4501 catagttccg tcgtcgttca tgacgttgac ttgtggcaag ttatgacgtt ggccaaccaa
     4561 gaagtcattt ggatcgtggg caggtgtgat tttcacgaca ccagtaccaa acttaggatc
     4621 tgcgtgctca tcaccaacga ttgggattaa tttattagca attggaagga tgacattttt
     4681 accaatcaag tccttgtagc gcgggtcttc tgggttgacc gcaaccgcaa cgtccccaaa
     4741 catggtctca ggacgagttg tagcaacttc aagggcgcgt gaaccatctt ccagcatgta
     4801 attcatgtga tagaaggcac cttcgacgtc cttgtgaata acctcgatat cagaaagggc
     4861 tgtgcgagct gctgggtccc agttgatgat aaactcacca cggtagatcc agcctttctt
     4921 gtaaaggtcc acaaagacct tacgaacagc ttttgacaaa ccttcgtcaa gagtgaaacg
     4981 ctcacgagag tagtctacag agagccccat cttgccccat tgttccttga tagtagtgga
     5041 atattcgtct ttccattccc agactttcgt caagaaagac tcacgaccaa ggtcatagcg
     5101 ggaaatgccc tcaccacgca agcgctcctc taccttagcc tgagtggcaa tccctgcgtg
     5161 gtccatccca ggaagccaaa gggtatcaaa accttgcatg cgtttttgac ggatgataat
     5221 atcttgcaaa gttgtatccc aagcgtgacc aaggtgaagt ttacctgtaa cgtttggtgg
     5281 tggaataacg attgaataag gcttagcctt ttgatcgcct gaaggcttga aaacatcagc
     5341 atcaagccat ttttggtaac gaccagcctc aacctcggct ggattgtatt tagatgaaag
     5401 ttctttagac atgtgtgtgt cctttctcta ttttgtttat tttattttga atttgcttag
     5461 cagcttcttc tgcagacaaa ttcgtattat ttattttaaa gtagtggtgc aactcattcg
     5521 gttgatattg ggaatttaat tgaagtgttt cagcggtctc taaaatttct ctttcagata
     5581 cctcaatatg tcgttttaag ggtttgtgct ttaatcgatt ctccgttcga tttcgacgta
     5641 tgcactcttc aagacttgtt tccaactcaa caaaaagaat ctcttgatga taatcatcca
     5701 acaaattctg aatttgcttt aaatacatca gttgatactg atttgaaaaa tcaattacat
     5761 ctgttaaaat tactgatcgc tgatttcttg cacttgctcc aaggaatgaa aaagtgattc
     5821 cacgaacaaa ttcccacatt tcctctgtat agtcctgata gatttctagt gcaaaatcaa
     5881 tggcttgatg gttataaaat agggtagcat ccgtcagtcg agataattct tgaccaatgg
     5941 tcatttttcc tgatgctgga gcaccaatga tgaaaagatg catcaaatca cctcccactc
     6001 actcttcagc aaggcatatc tcaaatcatc acagcagttg ccttgagcat ctttacggtc
     6061 tcttatgcga gcttcgaggg taaagccaag cttttccgcg actcgttgac tttgaaggtt
     6121 atatccaaag caagttagtt caatcttgtg aagacccaaa tctttaaagg ctaagtcaat
     6181 caaggcacgc gcagcttctg gcacataacc tcgcccccaa tagtctgggt gtaaggtata
     6241 gccgatttcc agcacatcat cttcatggcg atggttgaaa tcgacagagc caacgatctt
     6301 atccgttcct ttgacgacaa tcccgtagcc tgctggatag gcgacctctg gcaaactagc
     6361 atagtcaaag atatcctcag catccgccac agtacggact cgtaaaacga gacgttctgt
     6421 ttcgatttta tctggcagct cagttcttgc catccttctt cctcgctttt ttgatgaaac
     6481 tgcccttcat atctacacgc ttatccagat agcgataaac gcgctgatat ccatctccca
     6541 tgaaataggt tggggcaaac agttgatttt taaaatgtcc cttttcatcc aggagttctg
     6601 gggcaacaag tcgctcaaga atcttggcaa agatgtggca aataccgtct tcctcaacaa
     6661 tcctatctac ccgacaatct aaaacaagtg gacaggcgtc taaaatagga gtctgagttc
     6721 gttcagaaat ttcataatgc actcccaaac gttccaattt ctcctgatga ctgataaaac
     6781 cagcctgctc catcgcaagc atagaagttt catcagaaat attcacagta aatttttgat
     6841 actgtttgat ctgctctgcg gcattctctc tcgcaacgac tccaatcaca acccaatctc
     6901 ctagactata cgaggaacta caggtcgtga tgttatagcc aaaattctga tcttgatatc
     6961 ctaaaataaa aacaggaaaa ccataatata gtttattagt tttaaatgat tgttttataa
     7021 caacctcctt tgactaagac acaaaagaaa agccctgcca tctacatgac agggacgaat
     7081 gtatttatcc gcggtaccac ccaatttcgg gccaagcccg catctttagt ctttaaaata
     7141 aaaagacaga aatatttcat tttttcagtc attagcaacc agttttgaca catttgtgga
     7201 cttctcagcg ccgccacttt ctgtaaaatg tgggattcaa aacacctcta atagctctat
     7261 tgtagcaagt ttttctcgga aatgcaaggc tcaagaaaaa ttacttgaac ttgatgccat
     7321 tttctttcaa tttcttgatg gtagctgggc cgattccttt caaggcaagg agttcttttt
     7381 cagtccagtt tttgaaatct gaagcagact tgattccttc atcatagaaa gtcttggcac
     7441 gatcaagtgg aagtccaccc aagtttgctg caaaatcttc tacagaatta gctacttttt
     7501 gagcttgctc tttggtagct tctactgctt gttcaacttt tttagaaaca gccttaccag
     7561 ctgcgcttgc tgactgttct gcacgcttca cgactttttt tgtctcatct acaaccttag
     7621 tcacagtact tgaaaaggca cctgcgcgac ggaggccatt tcgtaattgt tttttacgat
     7681 tgagtttctt tgacatgata aaatcctttc aataaaaaat ccctgctctg acaaggattt
     7741 aacataaaca ttctatcaag attttagtga aaaatcaaga atctttctcg tttaataatt
     7801 tcgctcacca aacaagcctt ctggcaaatc atggaatccc ttgcctactt tgaagttttc
     7861 aaacttacca agcaagaact gataagcatc caagcgactc tcgtagcgaa tcatggcatc
     7921 tttggcacgc tcgtcctggt cttcttcgta aactttttga ctttccttgt gcgccatgtc
     7981 gttaatcatg atttgggtat tgacccaagc ttcaaattcc ttcataaatt cttgttcgta
     8041 actcattttt ctccttattc taatccacga aaatagtccg tatcaatctc acggtagggc
     8101 tggatatcct gaacatactc caacactcct tggaattctc cgtcttcatc gtgtactgca
     8161 gcataggtga tgtggacaaa cttacctcgc gactcagact tgaaccacat ttcatacttg
     8221 tctttgcttc cctcacgaag cctcttcatg atagttttga ccttgtccaa gtacttaggc
     8281 ggatggcaga gttcgacatt gcgcccgact tgagacggcg tccgtttgaa aatcatctca
     8341 tcagctggcg tattgtcatt gtaatactgg aaaatatctt ctttattgac aaaggtaatc
     8401 tccataggga gatgattgag gatgagattg gcctgctcga ctgaaagata gccattacca
     8461 aaagcctgtt gactatggcg gtccagcaca gcttcctttt ccttaggggt aaaggtaatg
     8521 gtaaaatggc cttctggcgt atctatgact tgttgaactt gaccttctgc cgtatctagc
     8581 tgtacaggct cctctgcaat cttttcctca ataaagctct gtcgttctgg cacccatttc
     8641 tctgacggac ggatgatggc atagccatag gcatcgctct cctccgcaat ctgaagccag
     8701 tcatcctgag taaaagactc aaggagaatc atgaggagga tggactcttc cttgaaaatc
     8761 atactttcaa actctgtcgc aaaagcttca aaagcttcct ttacactgct aattgacact
     8821 tctggtagtg acttgactgt cgttagagct gtttgaaaga gttccctaat ctgatcatcc
     8881 actccccaca taactttggg aggtgaatcg tgtccatagc gctccatgat aggaaagaag
     8941 agttcttcct tacgttggta atggatgtca aattgcccca caagccccat ctgacgcacc
     9001 aagcccttgc gcatctccgc aagcatttcc tcgtcttcca tcaactcata ggtatctaac
     9061 aatctacgaa tgcgaatcaa agctgcacgg agggccaaat tttcttcctt aaagacacga
     9121 actgggtgcc cagggtgttc ggtatctaaa acttcgacac ctttgatagc atttttaaaa
     9181 agattggcat ggacatcaca gagttccata acatcttcaa aagtgacgcc cgaatccgag
     9241 ttcatcagct cgtgctccat aagggaaatc tcgatggctg acacgcccgt aaaggtcgca
     9301 tcaaagcgat cttgaaccga ctcaggagag gcgccattgt gcaattctaa caaaatatcc
     9361 cgtaggatat gaatccattc atctgtcatt agtctaatcc aatcacttcg tagccattcg
     9421 cttccagtgt gcgtacaatc ttgtccatag gagttcctgt tagcttagaa ccctgtttaa
     9481 gtgatacttt acgaccaact gtattgcgca ttaagggatt ggcaaggggt ttaaaaccca
     9541 actccactag aatttccaag acttctggat gcttgtccac cacttctgca acaggaattg
     9601 acacatcgat gatattgtcc atgacgacct ccattgtatg ttctaatact caatgaaaat
     9661 caaagagcaa actaggaagc tagccgcagg ttgctcaaaa cagtgttttg aggttgtgga
     9721 tagaactgac gaagtcagta accataccta cagcaaggtg aagctgacgt ggtttgaaga
     9781 gattttcgaa gagtataaaa tgattttact gcttatatta taccatatgg agagagatta
     9841 aaaaactagc agtggagttc ctgctagttt tctgcgactt ggattaccat ctagataagt
     9901 ttaaagaacc aatccaatat cttgtaaaac agcaattgaa ctgcaaaaga aatgatcatc
     9961 caaaagaatt tcacaatccc aataatcggt ttgataaaaa gaatagcaag aattgtccag
    10021 aaaaatttca ttctctctcc tctggcttaa tgagccaccg agccgtatcc attagcttat
    10081 ctgcaataaa gagttttccc agaccaacaa cgacatggtc atctttcttt atcgttttca
    10141 tcacttttac accttgcacg gtcaaaaagt aattaccata aattttagca aaagctttga
    10201 taggattcat attattctcc tttgatgatt tcagcctctt ttcggattgt ttcaacatct
    10261 tcttgatatt gaacttcact atagttgact agcttggata attctttctg caagctttcc
    10321 cccattggtt tcatagttgc aaaggttaaa ccacctgaaa tgataccacc caagatagga
    10381 ataaatttcc ccattccttt ggccaaccct cccttggtaa gattcacacc aaatattttt
    10441 aagacttttt tcaaaatagg gtaccaaagc gtctttgtta aagctttatt aggcactgtt
    10501 ttcattacct gtttggcaat tgttatacca ccagcacgta gcaaacaaag cagcggttcc
    10561 attcacccct aacattacgc ctagataaag caagagggta ttttgagcat cttcactcaa
    10621 ctcctcacgt gaagcccaaa gatcctcata accataaata taacctaatt cttgggccaa
    10681 tttcagagag aaaccataaa attgagccac ataagctgga atggtaattg ccatagcaag
    10741 ccctccaggt aatcctgcca aaacagaagt cccactcgct aataaaacat tatccctaat
    10801 acaggcatta gccactcgat ctaagatttc ttgagataag agagacgttg gaccttgttc
    10861 caataaggta ggaatgtcct gtggacccaa ttctttggaa aacttatcca ctaaaaattt
    10921 cgaacgatca accttaacaa ctggtagttt taccacttgt tgtaatactt gcatagctaa
    10981 gtcttgttca gccatataca aactccttat ttcttttttg caccttatca tattatattt
    11041 cctacaaaaa aagcattttc taattagata acattaaaaa atacagaacc ccttttttat
    11101 agtaaaataa aacaagaaca ggacaaatcg atcaggacag tcaaatcgat ttctaacaat
    11161 gttttagaag tagaggcgta ctattctagt ttcaatatat tttaaaagaa tatttttcca
    11221 tttcaaattc aacctatttt cctccaccaa aaaagggctc tataatattt gtagtgggta
    11281 aattccctat ggatattatg gag
//
LOCUS       DAVBCB010000061        11129 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVBCB010000061 DAVBCB010000000
VERSION     DAVBCB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11129
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.061"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..947)
                     /locus_tag="VQU28_001798"
     CDS             complement(<1..947)
                     /locus_tag="VQU28_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2477103.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQVIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFILVTFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTI"
     gene            complement(1027..1890)
                     /locus_tag="VQU28_001799"
     CDS             complement(1027..1890)
                     /locus_tag="VQU28_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HET2477104.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(1893..3794)
                     /locus_tag="VQU28_001800"
     CDS             complement(1893..3794)
                     /locus_tag="VQU28_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2477105.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNDLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENAAV
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINCVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
     gene            complement(4222..4497)
                     /locus_tag="VQU28_001801"
     CDS             complement(4222..4497)
                     /locus_tag="VQU28_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HET2477106.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(4603..5442)
                     /locus_tag="VQU28_001802"
     CDS             complement(4603..5442)
                     /locus_tag="VQU28_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2477107.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVIVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            5584..6432
                     /locus_tag="VQU28_001803"
     CDS             5584..6432
                     /locus_tag="VQU28_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HET2477108.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSGWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(6525..7442)
                     /locus_tag="VQU28_001804"
     CDS             complement(6525..7442)
                     /locus_tag="VQU28_001804"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HET2477109.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            7879..8084
                     /locus_tag="VQU28_001805"
                     /pseudo
     CDS             7879..8084
                     /locus_tag="VQU28_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQU28_001806"
     CDS             complement(8395..8688)
                     /gene="rpmA"
                     /locus_tag="VQU28_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HET2477110.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(8705..9049)
                     /locus_tag="VQU28_001807"
     CDS             complement(8705..9049)
                     /locus_tag="VQU28_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HET2477111.1"
                     /translation="MIQAVFERAEDGELRGAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENY
                     SEFVQTRVITEN"
     gene            complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQU28_001808"
     CDS             complement(9065..9379)
                     /gene="rplU"
                     /locus_tag="VQU28_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HET2477112.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYIEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(9415..9502)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(9597..10196)
                     /locus_tag="VQU28_001809"
     CDS             complement(9597..10196)
                     /locus_tag="VQU28_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HET2477113.1"
                     /translation="MTFCAGYVDAYTFIVRGNTLVAGQAGNVVFLSVELIQNNVSDVR
                     DKLLTLITFIMGVFLLTIYKEKLRIVKKPILSLIPLAILSIIIGFVPQTVDNIYLVQP
                     LAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLAFGDYFRTKHTPFLREGFIFVSLL
                     SSFVLGDVFSAYLTIFYHEKTILGVPIMMSIFYLSMLFA"
     gene            10666..10965
                     /locus_tag="VQU28_001810"
     CDS             10666..10965
                     /locus_tag="VQU28_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HET2477114.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         3509 a         2305 c         1855 g         3460 t
ORIGIN      
        1 aaaatcgtca taaacaattc acaaactaaa tttccagaaa aggaaagaaa agttggaagt
       61 ggtaatccca tcataaaaac tccgacacct gtcaaagcca gtaaaatcaa aagattataa
      121 atattagctt taattttact agctagaaga gccccaatga tggaaccaat agcccccata
      181 gttaaaatac ttgcataggc tccttctgac ccgtaaagct gattcgaaaa gggaagtaga
      241 aattcaaaag ctgcaaaaaa gaaattaacg ctggaagcta ccagcaaaag gaagaaaatt
      301 tcttgctgat gccagatata gtgtaaccca tccttgatat ctacaaaaat atctctccca
      361 gtaaaagcct ttttctcttg aacttttgct tcctcttttg gaaggaaagt cactagaata
      421 aaagcaatga aaaaagtcag cgagtctagc agtagcgtca tatggagact tgcaaactgt
      481 aaaacaagga aggaaagaac aggagagcta acacctacaa cctgcaaaac cagctctaag
      541 cgagaattat agatcacaat ctcatctttc tccaccactt cagttatgat agctttattg
      601 gctgtgcgag aaaaggcaaa agcaataacc tgcacaatgt tagcaacaat caaagcgcca
      661 atcatccagc tatcattcct tatgaaagaa atagccagac aaagaatccc acaaacaaga
      721 tctgccgtca ttaaaatctt acgacgagaa aaacggtctg aaataactcc gccaaaggga
      781 ttgacgagaa tagatgtgac gagctcagaa atctgataca ttcctaaaac tgtctgtcct
      841 atagtcccca tagaagccaa ccagacacta tttccataat catagagcat atttcccatt
      901 ttattgatag ccccacggct aatcaactgc actgcatagc gattcatatt aaagctcctc
      961 tcaaattttg aaactattgt atcaaaaccg aaaggagctt tttatttttt cccttatttg
     1021 ggaaaattaa cttttgacaa atttttcgta gtgttcctga taataggcta cttgctctgg
     1081 aagacctaac acatcaaaaa tatgcatggc ctcttgcatc tgcttacagc cttctttaca
     1141 ctgtcctttt tgatataagg caaaaccttt taaataatgg aaaacattac gctcataaag
     1201 cttaatacct ttgtcaataa tcttctctgt ataagcctca aaatagttgg cattataaaa
     1261 agaagaatgc tctaaacaat gctggtaaca attgagggcc aaaatcaaca ctaatctctt
     1321 atggcgacta atctcttggt aaaattcctc cctctccata acttctctac caatccgagt
     1381 gacatagtct acatcgtaga aactatagag gttaccgaaa agaatcaact catacatggt
     1441 ccattcttct gttttgaaga gataatctgc taccttatcc aaatcatcct gcttcatatc
     1501 ataactcgca tctctttgac aaatcagacc ttgtagcaaa atccagttca gctcaaaata
     1561 aagggaagtc gtcgaactct tagacttttc aagttgttct ctttgaagct tttgaaaacc
     1621 tgcaatatcg tttgaatagt aaagtgggat aatctgtgcc atcatagaca catgttcatg
     1681 attatgaaaa ttccttgcct tatccatgaa attttcgatt gttacatgaa tgttatccaa
     1741 aatctcaaag aaacgggaga ctgccaggtc agactcccca agctcaaagc gagataactg
     1801 agaggtagag caggattcgc ctgctgcttc ctttaaagaa taatttccac ttgttcgaaa
     1861 ttcacgaaat acttttccaa gatgttccat ctttacacct gatctgataa ttcttcccac
     1921 tcaagcatag cttcttcctg acgatggctg attttatcca gctcagcctg taattccatg
     1981 agtttgtcgg catcgtttgt ttccaacatt tgttcagaaa tggcttggct ttgactttct
     2041 agctcttcaa tttcagcttc tagactttcg atttgtcgca tgagtttgcg aacttctttt
     2101 tgactttctt tctgggcctg atagtcattg actggacttg cttcctttgc ttgattgcta
     2161 gttgaagctt cctcagtctg actcatttct gctgttgctt tcttctcaac atagtagtcg
     2221 taatctccaa ggtagagagt tgaaccattc tcagacaatt ccaaaacatg agttgccaca
     2281 caattgataa agtaacgatc atgactgaca aaaagcaagg taccatcaaa gtcaatcaag
     2341 gcattttcta gcacttcctt actatcaata tccaagtggt tggtcggctc atccagaatc
     2401 aaaaagttat tgttttccat agacaattta gctaaaagca aacgagcttt ttcgccacca
     2461 gatagcatgc cgactgattt tttaacatca tctcctgaga aaaggaaggc tccaagacgg
     2521 ttgcggattt caacttctgg tgtcaatttg aaatcattcc agagttcatc cagcaccgta
     2581 ttacttggtg tcagcttgct ttgggtttgg tcatagtaac caacctcaac attagcgcca
     2641 aagcgctttt ctcccttgat aaacggaatt tgatccacga ttgatttgat aaaggttgat
     2701 tttccgatac cattgggacc aacgatagca acagcattca tcttacgaag gtctaggtta
     2761 atcggttgtg acaggacttc cccatcatag ccaacagccg cattttcaac agtcaaaaca
     2821 acattgcccg acgttttttc agactggaag gtcatgttgg ctgctttctt gccagcttcc
     2881 ggcttgtcca aacgctccat tttttctagt tgtttacggc gagattgagc acgtttggtc
     2941 gttgaagcac gaactagatt gcgattgaca aagtcttcca gagcagcgat ttccttctgt
     3001 tgcttttcat agttttttgc ctcagtagct agcttttgct ccttcaactc gacaaaacga
     3061 gagtaattcc ccacatagcg atccaaggaa tgcttggtca aatctagcgt aattgtcgca
     3121 accttgtcca agaaataacg gtcgtggctg acgataatga gggcaccgct atagtttacc
     3181 aagtaattct ctagccagga gatggtttca atatccaagt ggttagttgg ctcgtccaag
     3241 accaagagat tgggcttttc aaggagcatt ttagctagag ccaaacgagt attttgacca
     3301 cctgaaagtt cagctatttt catctgccac atagactcgt caaacttgaa tccattcaaa
     3361 atcgctcgaa tatcagcttc ataggtaaag ccacctgctt ggcgaaaatt ctcagataag
     3421 cggtcataat ctgacatcag tttatccaaa tcctcaccag acttttcacc catctccagc
     3481 tccatctgac gcagttgtct ctccgtccga cgcaaatcat taaagacatg aagcatttca
     3541 tcgtagatgg tattttcaga ctcaaaacgg ctatcttggg ctaggtaaga cagagaaata
     3601 tcttttttct tattgatttc tccgctagtt ggctcctctt ctccaactaa aatcttcaaa
     3661 agagtagact tacctgcacc atttttccca acaagagcaa tccgatctcg ttcatcaacc
     3721 tgcaggttga tattatcgaa aagaacctct cctgcaaaag aacgttcaat tttattagct
     3781 tgtaaaataa tcataagagt agtatagcat gtttccctaa ggcattcaag ataatcgtaa
     3841 gtcttttagt acaactttta taacataaaa taaactaaat tatgtatatt ttatattaga
     3901 ttccttcact atcttgttgg attttctaac cagctaatct tgtttcaaat agttatcgca
     3961 caagcctatt atttaattct tttcattatt tacgtacgcg tatagcagat tgaaataaga
     4021 tgagaacaaa tcgattggga aagtaaaatt aatttctata aatgttttag caattgtttc
     4081 gtactatttt agattcagtc tactatgtac aatattttcg gaacattcaa ctttttaact
     4141 ctatttatta ctagatttca taattaaaaa gcctactgac caagctagaa agcttgatac
     4201 aataggcttt ttaaagactg attatttaac agcgtcttta agagctttac cagctttgaa
     4261 tgctggtact ttagaagctg caattgtcat ttctttacca gtttgtgggt tgcgaccttt
     4321 acgttctgca cgctcacgaa cttcaaagtt accaaaaccg atcaattgaa ctttttcacc
     4381 agctgcaaga tagtcagcta ctgctgcaaa tacagcttca actgctgctg ctgagtcttt
     4441 cttagtcaat tctgtagctt ctgctacttt agcgatcaaa tcttgtttgt ttgccatgtt
     4501 aatgattcct ccagataatt tctaattaac aaatataatc atatcctaaa atccctttta
     4561 ggtcaagtta aaaacgctat ttcttccaat tttatttatt ttttaggagt ggtaacgtat
     4621 caaaatagcc caagcgttct cacccgtgtg agtttgaata atggaacccg tttccaaaac
     4681 agaaattggc ttttcaacat aagcttgtaa gctttctttc atctcttttg cccaatcatc
     4741 actaccagaa tatgaaattc caatctctgc tacagcacgt tcagaaagcg atgttatcaa
     4801 ctcatctaac cattttttaa atgttttagt tccacgacct ttaaccattg gctgcaattc
     4861 atggtctttc atttgcatga caacacggat attgagaagg gagctcaaca agccagttac
     4921 acggccaatt cgtccacctt tgacaagatt ttccaaagtt gaaacaccaa tataaagctc
     4981 tgtatggttt ttaacctctt ctacatgaga taaaattgcc tccatatctt taccttcttg
     5041 agctaacttc gcagcctcaa caacttggaa tttcagggct tggtcagtga aggaactatc
     5101 aacaacaatc acatctgcag tagataggct agcaccttgg cgtgctgctt ctaccgtacc
     5161 cgaaagagca tgggacatat gaatagcaag aatctggcca ccatctttgc ataggtcttc
     5221 aaaaatctca gcaaagacac ctacaggtgg ctgacttgtt ttcggaagat tcttactttc
     5281 ttgcatcaac tgaagaaatt taccttcttc tttcaaatcc gcatcagaat aaacaacatt
     5341 atcaatcatt acagataatg gaacaattgt aatatctaat tgctttacta gttcaggttc
     5401 aatagtaaca gatgaatcgg ttacaatctt aatttttgtc atagtatcaa tctttctatt
     5461 ttaggattca gattggtttc cttacttcta attatatcaa aaaaaagatt aaaaatccta
     5521 atggagtcaa tcaaattttc cgtaaaattt gatataatca acttataaga aaagaggtgt
     5581 cctatgatta aaaaaattta ccccattttt accattttac taggtgctgc tatttatgct
     5641 tttggactga cttattttgt agttccccat catctctttg aaggaggggc gacaggtatt
     5701 accctcatca cattttatct ttttaaaatc cctgtttccc tcatgaacct gctgattaat
     5761 attccccttt tcatcctagc ttggaagatc tttggagcca aatccctcta ttctagttta
     5821 ctaggaacct tagctttgtc cggctggtta gctttttttg agcatattcc ccttcatatt
     5881 gatcttcaag gtgatttact aataacagcc cttatagcgg gaatcctatt gggaattggc
     5941 cttggaatta tttttaatgc tggaggtaca actggcggaa ctgatattct agctcgtatt
     6001 ctcaacaaat acactcatat atccatagga aaactgctct ttatcttaga tttttgtatt
     6061 ctcatgttga ttctcctaat cttcaaggat ttgagattgg tttcctacac gcttttgttt
     6121 gattttattg tttctcgtgt tattgatttg attggtgaag gaggatatgc cggcaaaggc
     6181 tttatgatta tcacaaaacg tcctgaccaa cttgctaagg cgattaatga tgacctcgga
     6241 agaggtgtta cttttatttc tggtcaaggc tactatagta aagaaaattt gaaaatcatc
     6301 tactgtattg tcggaagaaa tgaaattgtg aaaacgaagg aaatgattca tcgaatcgat
     6361 cctcaagcct ttataactat tacagaagcc catgaaatcc taggagaagg cttcaccttt
     6421 gaaaaagaat aaaaagaggt aatgtcgtga cctcaaaagt tagactaaat catctatctt
     6481 ttgggttaca gacaacctct tttttgtttt atttactcaa gctcttaaga ccaattccga
     6541 gttacttctt catcagcctt taactgatcc actaattggt caactgagtc aaatttggtc
     6601 atatcacgaa tgcgatcaag ccaataaacc atgacggttt ccccataaat atcttgatta
     6661 aaatcaaaaa tattgacttc aaaacgtgct tcttctccat caaaggtcac atttttcccg
     6721 acactagcca tagcacgata cttctgtctt tgaatctcaa catcaacgac ataaacgcca
     6781 tctgctggca tataagtacg gtctaaaagc actaaattcg ctgtcggata accaattgta
     6841 cgaccacgag cattaccatg aaccaccata cctcttgatg gaagcggtgc ccccaaaagt
     6901 tttcctgctt ctttcacatt tccatctaaa atagcttgac ggatacgagt tgaactaatc
     6961 tttcctttct catcttctac aggtggaaca atgataactt ctccatcaaa gtaatccttt
     7021 aaatcttctg ctgttttttt gtcagaacca aatgtataat caaaacctgc aacaataatt
     7081 ttggcattca tagccttgat ataagttgca aagaattctt gtgcagtgag actagcgaat
     7141 tgactactaa aatcaaggag atataattct tctacacctt cgcgctttaa ttttctttca
     7201 cgttcagcag ggttcaaaat atgcaaaaac aaatccggat gataaggctc taaagcgatc
     7261 tttggagatt cattaaaggt cataacgacg ataggtaata aatcctttct cgcagccttg
     7321 ttggcaacac gaaataattc ttgatgcccc ttatgtatgc catcaaaata gccgagaaca
     7381 acgactgaat cagatggtgt gccaatatct ttttggtttt ttataggaat agtaataatc
     7441 ataaaataat tatatcatag cgatagctat ttctggaaca gaaaatctga aatgttgttt
     7501 ttttcacatg aagtgtacct gttttcaaaa agcactttat tctatcgttg cttaactatg
     7561 aactttgcaa tattcttctc aaaaacttgt aggacatctt caaaattttg caaggagtga
     7621 ttagacttgt tcggtaacca taaagtgtca tactatgtta tgtatgaaaa agcaatgcaa
     7681 ctaactcctg agaactttaa attactaatt ggtgccgaaa aggtagaatt tagaatcgag
     7741 gtacacctat ggctgtaaaa tttacaaaat gagacaactt gggcaagatg tttgaagaat
     7801 ttcctaaact ccctgatttg aagcaagtca ctttccctga tgacaaagaa aaaagccaaa
     7861 acagcaaaga aaaactagat gactgctttc caacaactcc catctagtgt gcttcagact
     7921 gggctatttt tctctccatc tgttagcttg gattctcaga ccgtttcagc taaagaatat
     7981 cttttccctt atcagaagga acggctcaaa ccattcagac aagtgaaggg acgacaagcc
     8041 aatatttgaa accagatagc agttcttata gtcaattgaa ataaaatctg aagaaatcga
     8101 gtaggaaact catatcaatt tttaacagtg ttctattcca gattcatact caatgaaaat
     8161 taaagtgcaa actaggaagt tagccgcagg ctgtacttga gtacggcaag gtgaaactga
     8221 cgtggtttga atttgatttt cgaagagtat caatccacta tacatattaa aaagtatgga
     8281 tatcaaaaca aaaaaacagg ctctccgaaa actcggaaag ccttatttta agctgtttct
     8341 agcttcctca cctttacatt tcaaggttcg ggataaaagg ttcaatggac ctttttattt
     8401 agcgattggg taaacagaaa cttgtttttt atcgcgtcct ttacgttcaa agcgtactac
     8461 gccttcaact ttagcgaaca aagtatcgtc tccaccacga ccaacgttta cacctggata
     8521 gatgtgtgta ccacgttgac ggtaaaggat tgatccacct gttacagttt gtccgtcagc
     8581 tgctttagct ccaagacgtt ttgcttgtga atcacgtccg tttgatgtag aacctccacc
     8641 ttttttgtgg gcgaaaagtt gcaagttgtt aagagtcatt tttaacataa tgttttcctc
     8701 cgtgttagtt ttctgtgata actctggttt ggacgaactc agaatagttc tccgataagt
     8761 ttgccatacc taagaaaaat gattcaaaga ataactgggt catttctctc tggtgtgaag
     8821 gaagatcttt tggtatttca accatcagat agccaccttc atcttcgttt aattctagga
     8881 ttggttcata gcctgcaaat ttctcaatag aattgataaa gttaatggca agcgtagaaa
     8941 ccgatgcaca cacgacatct aagccgtatt cgccactctc ggcgtgtcca gtaatttccg
     9001 cacccctcag ctcgccatct tcggctctct caaagactgc ttgtatcatg tgttctcctt
     9061 aaaattaagc gttaattgcg ttgatgacaa cttttgtata tggttgacgg tgaccttgtt
     9121 tacggtggct acctttttta ggtttgtact tgtaagtaac aactttcttt tgttttcctt
     9181 gtttttcaac agttccaact acagtagctc cagcaacaag tggagttccg acaacagtgt
     9241 tttcaccacc aacaagaaca acttcgttaa aagtaacttc ttgaccagct tcaacgttca
     9301 atttttcaat gtaaactgct tgaccaactt caactttaac ttgttttccg ccagttttga
     9361 taattgcgta tgtgctcatt atgcacctcc tatgattttt atgggtttcc ccgatttttt
     9421 gtgaagactc gcctagcatc gtgggacaaa ccacttaaac ttgaataatc aagcaacgat
     9481 gttcgtgcgg ttgcacagga tcgtgcatag tcaactcttc aagtatagca tatctcctat
     9541 tttcttacaa gtaataacac ctaaaatcaa gctttttctt ttactttttt ctgccactag
     9601 gcaaaaagca tactgagata aaaaatgctc atcataatag gaacaccaag aatggtcttt
     9661 tcatgataga aaatcgtcaa ataggctgaa aagacatcgc caaggacaaa actactaagc
     9721 aggctaacaa atatgaaccc ttcacgcaaa aaaggagtgt gcttagttcg gaaataatct
     9781 ccaaaagcta gcattgttcg tttgatattc cctgtcataa aggcattatt gtaggcaata
     9841 cccgaaacct caccaaaagc agttgtcacc agtcccatac agaaggccaa gggctgcact
     9901 agatagatat tatctacagt ttgtggcaca aaaccaataa ttattgatag gattgctaaa
     9961 ggaatcaagg acagaatagg ttttttcaca attctcaatt tttccttata aatcgttaat
    10021 aaaaagactc ccattataaa cgttattaag gttaaaagct tatccctaac atccgaaaca
    10081 ttattttgta ttagctctac cgaaagaaag acaacatttc cagcttgtcc agctacaagg
    10141 gtattccctc gaacaataaa agtgtaagca tccacatatc cagcacaaaa tgtcaaaaaa
    10201 gtgctaacct tttagactga cgtgatattt ttcttatagg taataacctc attttccctc
    10261 ccattgtatt ttctcttaga aatattgtac catttctttc taaaaaatcg tagactacca
    10321 tttagatttt actattagca taaaaataat aatagacaac tatttatcca aaaatagata
    10381 gaggtaacat gtttgcaaac aaagcatacg aacctttagt aaaatcattt ccatgaaact
    10441 agaatagagc ccctcttagc aaaaatcatt attttaattt atttctaatc actccttgac
    10501 ataaataact ctcaccaata aaagactatg tcttaaaaaa atggtataat aaaatcaata
    10561 cctgggcttg atggctatgc tactaataac aattaggaga gaaaatcagg cacttgttaa
    10621 caacaaggat tatccccttg agatgaaagg aactttagaa atcttatgat gaacatgcaa
    10681 aacatgatgc gtcaagcaca aaaacttcaa aaacaaatgg aacaaagcca agctgaactt
    10741 gctgctatgc aatttgttgg caaatctgct caagatcttg tccaagcgac cttaactggc
    10801 gataagaaag ttgtcagcat tgatttcaat ccagctgtcg ttgacccaga ggaccttgag
    10861 actctttctg atatgaccgt tcaagccatc aactctgctc ttgaacaaat cgatgaaact
    10921 actaagaaaa aactgggtgc tttcgctggg aaattgccat tctaaaaaca aggagctaga
    10981 acaatacttg tcgataacaa aggctaagaa aggtgcaaaa attataaaaa acgcatagta
    11041 tcaagcaacc taaaactttg ggctctataa tatttgtagt gggtaaatcc actatagata
    11101 ttatggagcc tattttattg tagaaaaaa
//
LOCUS       DAVBCB010000062        11086 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVBCB010000062 DAVBCB010000000
VERSION     DAVBCB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11086
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.062"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(6..890)
                     /locus_tag="VQU28_001811"
     CDS             complement(6..890)
                     /locus_tag="VQU28_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HET2477115.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(894..1280)
                     /locus_tag="VQU28_001812"
     CDS             complement(894..1280)
                     /locus_tag="VQU28_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HET2477116.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLISLMLEEQILTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(1273..2616)
                     /locus_tag="VQU28_001813"
     CDS             complement(1273..2616)
                     /locus_tag="VQU28_001813"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HET2477117.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYEVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEGLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(2698..3582)
                     /locus_tag="VQU28_001814"
     CDS             complement(2698..3582)
                     /locus_tag="VQU28_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2477118.1"
                     /translation="MIQVRNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHIEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKDGQVIGTCTVDLSTNT
                     NYFYGLAISEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQU28_001815"
     CDS             complement(3594..4211)
                     /gene="cysE"
                     /locus_tag="VQU28_001815"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HET2477119.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFIW
                     KHGFKLLARMHSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQU28_001816"
     CDS             complement(4227..6440)
                     /gene="pnp"
                     /locus_tag="VQU28_001816"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HET2477120.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDDQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRLELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHHKDHKPKKEFTETPKDSE"
     gene            complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQU28_001817"
     CDS             complement(7125..7991)
                     /gene="metF"
                     /locus_tag="VQU28_001817"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HET2477121.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIEFIKEQAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQU28_001818"
     CDS             complement(8055..10304)
                     /gene="metE"
                     /locus_tag="VQU28_001818"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HET2477122.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVASDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(10565..10831)
                     /locus_tag="VQU28_001819"
     CDS             complement(10565..10831)
                     /locus_tag="VQU28_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2477123.1"
                     /translation="MEGCPSELFSKEEILESDMRVAIMSELIEARYEQGISQKKLEEV
                     SGVSQPVIARMETGESSLQLDTVLKVLTSLGKTLAVVPLEQGKS"
     gene            complement(10915..11086)
                     /locus_tag="VQU28_001820"
                     /pseudo
     CDS             complement(10915..11086)
                     /locus_tag="VQU28_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3095 a         2540 c         2194 g         3257 t
ORIGIN      
        1 cttctttaca aaccaactat agttgacaaa gaacctaaaa tcaattgata acacaaggtc
       61 aggtcggtca actctttcaa ctgaagccct gtcaactctt cccatttatc aatcttgtat
      121 tggagagaat tgcggtgcag atagagttgc tgggctgttt tagtgagaac agcactattt
      181 tcccaaagag agagaatgat ttcctgaatc tgatcttgat ccaaaatcat ctggtgtaga
      241 cattctttaa ttggcttcaa gtccacgagt ctttctccca tactccaaag atagagctga
      301 gaaaaagtat gaacaccttg gtgaccctga cgccaccatg tcttgaacaa atcccgctca
      361 gctttgatta agtctgatag ggcttgatgt cctgtctgag accaaacctg acccaacatg
      421 atagaaagac gaagtccaaa gtcatactca actgcttcaa tcatatcact taaaatatct
      481 cttacagaag tgtatttgtc ttgttgaagc acgaaaacat aatcctgagc tccgacctgt
      541 aacactgtct gacaattcgg aaaaagagtc cgcatcatat ctagccaaga agccagattt
      601 tcctgctgaa aataagaaag atggcaataa accaactgaa tctttttaaa aacttgcggt
      661 gcctgtccct tgccttcaac cagataggaa taccaagggt ttagcgaacg agcctgctcc
      721 tgctgggtca aaagggcaac caactgcttt tcacgctcgc tgagcccagc ttcctccagc
      781 aaaatccact gctgagaagc taaaggaagc gtgagatagc cctctttctc tactggttgg
      841 tctgaaatcc gagcctcagg aaaccagtct tgtagttctt ttgccctcat gttctagccc
      901 tccacttttt ggatgcacca tgaaaccaaa ctctcaagac gttccagatt ctcagtcata
      961 tggagatagc ccataactgc ttcaaatccc gtggacatac gataagtcac gacatctgca
     1021 tttttagcct ttgtgtgact attggtattg cggccacgtt tgtagatttc ttcttctttt
     1081 tccgttagga tctgctcctc caacatgaga gaaatcaggc gagcttgagc cttggctgac
     1141 acgtacttgg tcgcctcttg gtggagttta ttgggcttgg tcataccttt gaggatgagg
     1201 tggcggcgaa tatacataga atacaccgca tctccctcaa aggctagcgc aatcccgtta
     1261 atgagattga catcaatcac gtgtccacct cactccatcc ttggtatcaa ggagcttaat
     1321 tccttgagta accaattggt cacggatttg gtctgctgtc gcaaagtcac gattggcacg
     1381 cgcctcttgg cgtttttgaa tcaagtcttc aatctctgca tccaaaactt cctcaacaaa
     1441 gacaattcca aaaatttcta acatatctgc aagagcttgc ttgacacttg catcatagtt
     1501 ccctgagttg atccatttgg ccatttcaaa gacaactgtg ataccgttgg cagcattaaa
     1561 atcttcatcc atagctgcta caaacttatc tttaaagttt tgtaactctt gggcatccac
     1621 gtttcctgta aatggttgtt cgtaagtatt cttcagatac ttgagattgg tctcggcatc
     1681 gcgaactgcc ttttccgtga agttgatagg cttacggtag tgctgggtcg caaagaagaa
     1741 acgaagtact tgcccatcaa gagttttaag ggcatcgtgt accgtaatga agttacccaa
     1801 ggacttagac attttgacat tgtcgatatt gacaaagcca ttgtgcatcc agtagttagc
     1861 aaaagccttg cctgttttag cttcagattg ggcaatttca ttggtgtggt gtggaaactc
     1921 taggtcagct ccaccaccgt gaatatcaat ggtatcacct aaaatctctg tcgacatgac
     1981 tgaacactca atatgccaac ccggacgtcc aggtccccaa ggactatccc aagaaatctc
     2041 acctggtttg gaagatttcc atagagcaaa gtctacagga ttttccttac gagccgtttc
     2101 ttcatcggta cgacctgaag cacctagctc caaatcttcc aaggttttat tagccaattt
     2161 agcatagttg tgggattttt ctacacggaa atagacatcc ccttgactct cataggcaaa
     2221 gcctttctcg atcaagcctt ccacaaaacg gatgatgtct gccataaact ccactacacg
     2281 cggatggcga gtcgcaggtt tcacgcccaa ggccgtcaca tcctcacgaa aggcagcgat
     2341 gtacttatcc gcaacctcct gaggcgtgat gccttcttcc ctggcacggt tgataatctt
     2401 atcatccaca tccgtaaaat tggaaatata ggcaacctcg tacccacgat actcaaaata
     2461 gcgacgaatg gtatcaaaag ctaccgttga acgagcattc cctacatgaa tatagttgta
     2521 caccgttggc ccacaaacat acatcttgat cttgccgtcc tcaatcggga caaattctcg
     2581 caaatcacga gacatggtgt catagatttt aatcataaat cataatcaag aaagctgaaa
     2641 tccaagaaca attagtttca tcactaaaag ttcaagtaaa tttcagtccg aatatctcta
     2701 cacttcggaa tcccttgctc ctttctcatt cagataaacc acctgagtct gtttgacaaa
     2761 gccaattttt tcatacaaac gtttggcacc tacattgcta tcttccactg caatctgaaa
     2821 ttccttgtca ttttgctcaa ttagttggtt gacgagggat tttgctaagt agcttccata
     2881 gcctttccca cgttcaggtt ccgatattgc taaaccgtag aagtaattcg tattagtcga
     2941 taaatctacc gtgcaagtcc caataacctg accgtctttt aacaaaatat atagtcggct
     3001 ttctggatcc ttcagagctt cagcgacata tctatccaca acttctctcg attcatgttc
     3061 ctctgaaaat gcctgaaatt ttaattgact aatttgatcc tgatacgaac tatctgctaa
     3121 caaaacttca atatgggaaa catttgctaa cggataaggt cttctatcct tacctaacca
     3181 agtttctgtc tcttcatcct cgattagtcc ccagttactg gcaaagtcag gatgattctc
     3241 taaaaaaata cgttctgtct gaaaagtgac tgatcgaatg gggaaagaag ctgtttctct
     3301 ctcaaaacta gtaaacaatg cacgcgcaat cccctgacgg cgatgacctg gatgaaccag
     3361 tatcgtcact tctacatctt ggtcatctgc atagacagtt aataaaccaa caagttcgcc
     3421 tttttcataa taaaggaaaa aggcgggcat gtttgggtca aaattaagca tgttagagag
     3481 atagggatcg cgataggtac cgtcatagtt ttggcaacag ttaattactt ttttcgcctc
     3541 agatagctcc tcttggctta acttgtttct tacttgaatc atataggtat cctctacaaa
     3601 ccagacgatc tgtgactggc atctttagcc tgctcgagtt tattgacata atactctcgt
     3661 ttttcttcga cttcgtgaat gacaggctca tctttcttac catgaagacg gacaatcttg
     3721 gccggaatac cgacaaccgt cacatcacta ggtacatctg ctacgacaac tgctgcagca
     3781 ccgaccttgg cattttcacc aatttccaca ggtccgataa cttgggcatg ggctgatatg
     3841 agggctccct ttcgtacagt cggatggcgt ttgccacagt ctttccctgt tcccccaaga
     3901 gtcactccgt gatagagaag aacgcctttt tcaacaatcg ctgtctctcc aatcaccaga
     3961 ccagaaccat ggtcaataaa gacacctgaa tcaatctggg ctcctggatg aatctcaatc
     4021 tgagtccaaa agcgccaaaa ctgactgtgc atacgagcta agagtttgaa gccgtgcttc
     4081 cagataaaat gcgagagacg gtgggccgcc aaggccttga cacctggata agtcagcaaa
     4141 acctccaaag tggtgcgggc cgctggatca ttttctttta cgatatcaat ggtttcgcgc
     4201 caccacccca tacatttctc cttttcttat tctgaatctt ttggtgtttc tgtaaattct
     4261 ttcttaggtt tgtgatcctt gtgatgacgt gggcggtgag gacgctcaga cttttcacct
     4321 ttttcatcat gctcaggttt tggcggacga ggtagaagag ccttcataga ggcatcgata
     4381 cggccttttt catcaatctt gataacctta acatcaactt catccccgat ttctaccaaa
     4441 tcctctacac gattggtacg agtccaagcc atctcagaga tatgaacaag ggcatctgtc
     4501 ttatcaaaga ggttaacaaa ggcaccaaat ttctcgatac gaacgacttt agcacggtaa
     4561 acttcatcca ctttggcttc acgaaccaaa ccagcaataa tttctttggc acggttaata
     4621 gcatcttggt cactagagta gatagacaca tttccttctt cgtctatatc aatcttaaca
     4681 cctgtttcag cgataatctt gtcgatggtt tctccaccct taccgatgac aatcttaatc
     4741 ttgtccacat caatcttgat cgtatcaatt ttcggagcag ttggagccaa ttctagacga
     4801 acttctggaa tggttgcttc aatgacatca aggatttcaa aacgcgcttt cttggcttga
     4861 gcaagagcct ccgtcaagat ttctgcagta atcccttgaa tcttgatatc catttgaagg
     4921 gctgtaatcc catcacgagt acctgcaacc ttgaagtcca tatctccaaa gtgatcttcc
     4981 aaaccttgga tatctgtcaa tactgtgtag ttatttccat ctgagataag tcccatagca
     5041 ataccagcta ctggcgcctt gattggcaca ccaccagcca taagggcaag agttcccgca
     5101 cagatagaag cttgagatga agaaccgttt gattccaaaa cttctgctac tagacggata
     5161 gcgtatggga attcttccaa gcttggcaag acttgagcaa gagcacgctc accaagggca
     5221 ccgtgaccga tttcacgacg acctggcgca ccgtaacgac ctgtttcccc tacagaatat
     5281 tgagggaagt tatagtggtg cataaagcgt ttcttgtact ctggatccaa accatcaatg
     5341 atttgagttt ctcccatcgg agccaaggtc aagactgaaa gagcttgagt ttgcccacga
     5401 gtaaagagac ctgaaccatg tacacgagga aggaagtcaa caaccgcatc caaaggacgg
     5461 atttcatcga ccttacgacc atcaggacgc accttgtctt ctgtaattaa acgtcgcact
     5521 tctgcgtgtt ccatttgttc caagatttca gccacatcac gcataatacg gtcaaattct
     5581 tcgtggtccg catatttttc ttcgtaaacg gcagtcactt ggtctttcac tgcttgagtt
     5641 gcagcttcac gggccaattt ctcttctact tgaactgcct tttggaggtc actgttgtag
     5701 gctgcaatga tttcagcttg caattcagca tccacgtgaa gcaattccac ttctgctttt
     5761 tctttaccga cagcagcaac gatttcttct tggaaggcaa tcaattcttt gacagcttcg
     5821 tgccctttaa gaagcgcttc caacatgatt tcttctgaca attctttggc accagactct
     5881 accatgttga tagcgtgctt agttccagct actgtcaatt caagaagaga ttgctctgct
     5941 tgttcttgac ttgggttgat gatgatttgg tcatctacat atcccacttg taccccagca
     6001 attggtccgt caaatggaat atctgaaata gacagtgcca aagatgaacc aaacatagca
     6061 gccattggtg cagatgcatt ttcatcataa gaaagcactg tattgatgac ttggacttca
     6121 ttacggaaac cttccgcaaa cataggacga atcggacggt caatcaaacg cgctgtcaag
     6181 gtcgcatctg ttgaaggacg tccttcacgt ttcataaagc caccaggaaa cttcccagcc
     6241 gcatacattt tttcttcgta gttgacttgg agtgggaaaa aatccccagt tgccattttc
     6301 ttagacataa cggcagcagt caagacagtt gactcaccgt aacgtacgac aacagagcca
     6361 tttgcttgct tagcaacctg accagtctct acaattaact cacgacccgc aaaagtcgtt
     6421 tgaaacactt gttttgccat tttaatcccc tttggattga tgaaattata cgccttgcct
     6481 acaaagatca agataccaag gacgtcaaaa gcaaagtaaa aataggaaac tgacgaagtc
     6541 ttcgatgaag acaagacagt ttatcttttt tacacagctt ttcggccgtg ttcaattaca
     6601 caagatattt tggacggttc ggcttgccga acatttctgt agaaaaatag gaaggtgacg
     6661 tcgcactcga cgagtgctag gaagcttatc ttttttccta agaaatgaga ccaaaattca
     6721 agtcatcaag ataccaagcc gtcaagcaac tcaaaggaag ataggaaatc gaacgacgga
     6781 gcgactgctc ctaggcagat ttatcttttt ccacagagtt gtaggcgtgt tcagttttca
     6841 agatacatca ttagaaaggt ttaatattaa agtatctaaa cctttcacgc taatcgctat
     6901 cgggcgatta gctaaatgct ttactaactc tttcgtcaaa taacatcgat ttgactcact
     6961 cgtgtcgtta aatcttacag tttaaatgca ttgtattatt taataccttc atctttgtat
     7021 caagtacgta cagaatctat tttatcatat ttttcttaaa aagtgaggtc tttaccatta
     7081 aaaaggaacc attcccctca cctgagaaga atggtttgct tttattatcc tagagactgg
     7141 tgattaaaca aggcatgggt tgcttgatgg atgtattttg ctgtatcagc attattcatc
     7201 gtatagagat gcacaccggc aacatcctga gttaccaagt ccacgatttg gtccactgca
     7261 taggcaagtc ctgctgctct gagcgactca gggtcatgct catacttgtc taagatggct
     7321 ttaaatttgc gtggaagatg gatattctca caagtcttca agagtcggag agcctgattt
     7381 cgattcagaa ttggcataat tcctgcatga ataggaacat caatcccagc caagatacac
     7441 ttgtcctgaa aatcatagaa gcgctcattg tcaaagaaga gctgagttac gaggctcgaa
     7501 cagcctgcat ccactttctt cttaagattt tgaatatctg aaatctgatt tggcgaatct
     7561 ggatgccctt ctggatagca agctccaata atatcaaagt gaggggcttg ttccttgata
     7621 aactcaatca agtcggttgc atagcggaaa tccttttgtg gttctacgtc tggaataata
     7681 tccccacgaa gagccaagat tttctgaacc ccaactttgt ccaagtcagc aatagtttca
     7741 gcaaccttgt ccttagttag ataaatagct ggcaagtggg caatggtcgg aatcgccaag
     7801 tcattttgga taaagtcagc caaacgaacc gtcgtttcct tgatattaaa tttattattg
     7861 ctggcagtta cactgataaa atggggagcc aactcctgca tatcctgcaa ggctgaaata
     7921 atgttaccat tacccacggc tgggtttgga gggaacactt caaatgagag tgacggtgtt
     7981 tggcgtgaca tatgtaataa ccttttctag ttgatttctt tttgaacaac cactgtatgg
     8041 agagaaatcc aatcttacaa tttctcacgc gcagctttag ctgcttcaac aaggcggatc
     8101 aagctttctt ttgtttctgg gataccacgt gttttcaaac cacagtcagg gttgatccaa
     8161 actttcttgc ttggcacttt agcaaggatg gcttcgattg tgttgtcgat ttcgccttca
     8221 tttggtacac gaggtgagtg gatatcgtaa accccaggtc ccacttctgt ttggaagttt
     8281 ttcgctttga gttcgtccaa gatttcaagg tttgaacggc tggcttcaaa ggaaataacg
     8341 tctgcatcca tgttatcgat agctgggatg atatctgtaa attctgagta acacatgtga
     8401 gtgtggattt gtgtgtctga cgctactgtt gagtgtacca agcggaaggc aggaattgcc
     8461 cagtcaaggt aatcttcgta ccagtcacta cgacggagtg gcaatttttc acgaagagca
     8521 gcctcatcaa tttggatgat tttcacacca gcagcttcaa ggtcaagcac ttcatccttg
     8581 atagcaaggg cgatttgaag agtagaatcc ttgatagaga tgtcttcacg tgggaatgac
     8641 cagttaagga tggtaacagg tccagtcaac atacctttaa caggtttgtt tgtacgactt
     8701 tgtgcatagc tagaccattt aacagtgata gggttaagac gagtgacatc accccagatg
     8761 attggtggtt ttaccccacg cataccgtat gattgtaccc atccattttt agagaagagg
     8821 tatcctgaca agttttgacc gaagtactca accatgtcat tacgctcaaa ttcaccgtga
     8881 acaaggacat caaagtcaat atcttcttgc cacttgatcc attcgtcaat cgtttcagca
     8941 aggaaagcat cgtactcttt ttgagacaat tcacctttac ggtaagccaa acgtttggca
     9001 cgaacttctt ttgtttgagg gaatgaacca atcgttgttg ttggaagagc tggaagtttg
     9061 aaagcttctt cttggatagc ttcacgttct gcaaaggctg gcaaacgagt gtagtctgcg
     9121 tctgtcaagc cagcgatacg cgcacgaagt tcagcatttt caccaacacg ctcagtcgca
     9181 aagagttctt tgttggctgc aagagcttct gaaccttgac cattgcggat agcatccaag
     9241 tcacggattt catccaattt ttcaactgca aaggcaaagt ggttcaagag tgctggttca
     9301 aattcttcat tagcagttgt aaatggcaca tgaagaagtg agcaagagct tgtcaaaacg
     9361 atgttttcag ctggaatttg ctcaagaaca gccaagctct tttcgtagtt gttgcgccag
     9421 atgtttttac cattgacaat acctacatag agagtcttgt cagctgggaa gccaccttta
     9481 acgagttcaa gagttttctt accttcaaca aagtcaagac cgatagcatc tactggtaag
     9541 tttacaaggt cagcgtatac gtcacgaaca tcaccgaaat aagtttgaag caagacttca
     9601 agaccttttt tgtcagccaa gagtttgttg taaaggttca agaagagagc tttttcttca
     9661 gctgtcaagt cttttacaag agccgcttca tccaattgga tgcgagtcgc accaagttca
     9721 gccaatttag caaaaacttc ttggtaagca gccactaagc tatctacgaa gtcgtctgct
     9781 ttcacgcctt cttcaaagtc tgacaattga aggaaagtga agggacctac aagaacagga
     9841 cgagtgttca atccaagttc tttggcttct tggaactcat cgaaaatctt gtgaccagcc
     9901 aattttactt gagtgtcttt ttcaaattta ggaacgatgt agtggtagtt agtgttgaac
     9961 catttcttca ttggaagggc gcgaacgtcc cctttttctc cctggtaacc acgtcccaaa
    10021 gcgaagtagc gctcaaggtc agacaagtcc aagttttgaa cggatgcagg caccacgttg
    10081 aaaaggaaag ccgcatctag gaagttatca tagtgagaaa agtcatttga tggaatttca
    10141 gtgatgcctt tttctttgac aatgttccag tgtttagcac gcaagtcttt tgctgctgct
    10201 aaaagttctt cttctgagat ttcttttcta aagtattttt cagttgtaaa ttttaattca
    10261 cggaattcgc ccaaacgagg gaaaccgatg attgtagttg acatgatgtg tcctccaaaa
    10321 tttgttgttg aaactatctt aacagaaaag aaagcgtctg tataattgta aaaaattagg
    10381 gtttgatata gtttgaaact atatatctgt ttcggacaaa agaaaaagac ttgaagcaaa
    10441 cgtctcaaat cctttgtaat tcttacttta cagctatatt ccaattagaa tactaaaaca
    10501 tgttattagt aattcttata agtgactatg acctgttatt agaaaagact ataactgatt
    10561 ctagtcaact tttcccctgt tcaagtggaa cgactgctag tgtctttcct agactggtta
    10621 gaacttttaa gaccgtatcc aactgaagac tgctctctcc tgtttccatc ctagctatga
    10681 caggttgact cactccactg acttcttcca gctttttctg actgattcct tgttcatacc
    10741 tagcctcaat caactcgctc atgatagcca ctcgcatatc actttcaagg atttcctcct
    10801 tgctaaagag ctcagatgga catccttcca attactccca atagcactat tcttcatcac
    10861 ttaaccctct ttcttttacg tctatgtatt tttaaaagaa tgagcgaatt atgattcgat
    10921 agattgacca gcaggtttaa agttggtgct agcctgtttc ttaagcgctt ttccttttct
    10981 agggtaaagc agatcctgct tgcttaaccc caattttcca cgatgaatcc aatagtaaat
    11041 ggttgaaatt cccacgttaa cccctttagc catcaccatc atttca
//
LOCUS       DAVBCB010000063        10989 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVBCB010000063 DAVBCB010000000
VERSION     DAVBCB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.063"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            136..2316
                     /locus_tag="VQU28_001821"
     CDS             136..2316
                     /locus_tag="VQU28_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2477124.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFTPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDTSQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKNLTEEVYSVADGQVVGLEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            2368..3183
                     /locus_tag="VQU28_001822"
     CDS             2368..3183
                     /locus_tag="VQU28_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2477125.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETLVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            3323..4666
                     /locus_tag="VQU28_001823"
     CDS             3323..4666
                     /locus_tag="VQU28_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2477126.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLEIVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            5043..6233
                     /locus_tag="VQU28_001824"
     CDS             5043..6233
                     /locus_tag="VQU28_001824"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HET2477127.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            6786..7721
                     /locus_tag="VQU28_001825"
     CDS             6786..7721
                     /locus_tag="VQU28_001825"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HET2477128.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            7755..8792
                     /locus_tag="VQU28_001826"
     CDS             7755..8792
                     /locus_tag="VQU28_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HET2477129.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNSSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKANSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            8965..9570
                     /locus_tag="VQU28_001827"
     CDS             8965..9570
                     /locus_tag="VQU28_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HET2477130.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            9726..10256
                     /locus_tag="VQU28_001828"
     CDS             9726..10256
                     /locus_tag="VQU28_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HET2477131.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQU28_001829"
     CDS             10277..>10989
                     /gene="rlmN"
                     /locus_tag="VQU28_001829"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HET2477132.1"
                     /translation="MKPSIHSLAHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQGIVQESADGTVKYLFELPDGMLIETVLMCQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFP"
BASE COUNT         3341 a         2017 c         2395 g         3236 t
ORIGIN      
        1 tagaaatttt ttagaaaaaa gtgtaatttt tttataaaag cctttacaaa aaaaattaaa
       61 gtggtataat taaatcatag aaggtgcaaa cgttttcgta aaacgtttgc ctaaataaaa
      121 ataaaggaga tttgaatgat gaaagataca ttcaaaaatg tcttgtcttt cgaattttgg
      181 caaaaattcg gtaaggcttt gatggtagtt atcgcggtta tgccggctgc tggtttgatg
      241 atttcaatcg gtaagtctat cgtgatgatt aacccaacct ttacaccact tgtcatcaca
      301 ggtggaattc ttgagcaaat cggttggggg gttatcggta accttcacat tttgtttgcc
      361 ctagccattg gaggaagctg ggctaaagaa cgtgctggtg gtgctttcgc cgctggtctt
      421 gccttcatct tgattaaccg tatcactggt acaatttttg gtgtatcagg cgatatgttg
      481 aaaaatccag atgctatggt aactactttc tttggtggtt caatcaaagt tgctgattac
      541 tttatcagtg ttcttgaagc tccagccttg aacatggggg tattcgtagg gattatctca
      601 ggttttgtag gggcaactgc ttacaacaaa tactacaact tccgtaaact tcctgatgca
      661 ctttcattct tcaacgggaa acgtttcgta ccatttgtag ttattcttcg ttcagcaatc
      721 gctgcaattc tacttgctgc tttctggcca gtagttcaaa caggtatcaa taacttcggt
      781 atctggattg ccaactcaca agaaactgct ccaattcttg caccattctt gtatggtact
      841 ttggaacgtt tgctcttgcc atttggtctt caccacatgt tgactatccc aatgaactac
      901 acagctcttg gtggtactta tgacatttta actggtgcag ctaaaggtac tcaagtattc
      961 ggtcaagacc cactatggct tgcatgggta acagaccttg taaaccttaa aggtactgat
     1021 actagtcaat atcaacactt gttagataca gtacatccag ctcgtttcaa agttggacaa
     1081 atgatcggtt cattcggtat cttgatgggt gtgattgttg ctatctaccg taatgttgat
     1141 gctgacaaga aacataaata caaaggtatg atgattgcaa cagctcttgc aacattcttg
     1201 acaggggtta ctgaaccaat cgaatacatg ttcatgttca tcgcaacacc tatgtatctt
     1261 gtttactcac ttgttcaagg tgctgccttc gctatggctg acgtcgtaaa cctacgtatg
     1321 cactcattcg gttcaatcga gttcttgact cgtacaccta ttgcaatcag tgctggtatt
     1381 ggtatggata tcgttaactt cgtttgggta actgttctct ttgctgtaat catgtacttt
     1441 atcgcaaact tcatgattca aaaattcaac tacgcaactc cagggcgcaa cggaaactac
     1501 gaaactgctg aaggttcaga agaaaccagc agcgaagtga aagttgcagc aggctctcaa
     1561 gctgtaaaca ttatcaacct tcttggtgga cgtgtaaaca tcgttgatgt tgatgcatgt
     1621 atgactcgtc ttcgtgtaac tgttaaagat gcagataaag taggaaatgc agagcaatgg
     1681 aaagcagaag gagctatggg tcttgtgatg aaaggacaag gggttcaagc tatctacggt
     1741 ccaaaagctg acattttgaa atctgatatc caagatatcc ttgattcagg tgaaatcatt
     1801 cctgaaactc ttccaagcca aatgactgaa gcacaacaaa acactgttca cttcaaaaat
     1861 cttactgagg aagtttactc agtagcagac ggtcaagttg ttggtttgga acaagtaaag
     1921 gatccagtat ttgctcaaaa aatgatgggt gatggatttg cagtagaacc tgcaaatgga
     1981 aacattgtat ctccagtttc aggtactgtg tcaagcatct tcccaacaaa acatgctttt
     2041 ggtattgtga cggaagcagg tcttgaagta ttggttcaca ttggtttgga cacagtaagt
     2101 cttgaaggta aaccatttac agttcatgtt gctgaaggac aaaaagttgc agcaggagat
     2161 ctccttgtca cagctgactt ggatgctatc cgtgcagcag gacgtgaaac ttcaacagta
     2221 gttgtcttca caaatggtga tgcaattaaa tcagttaaat tagaaaaaac aggttctctt
     2281 gcagctaaaa cagcagttgc taaagtagaa ttgtaatata cttgaggttg gaagctgtat
     2341 tccaacctct tattttggga gaaaagaatg aaatttttaa cactcaatac tcacagttgg
     2401 atggagaaag aagcagagga aaaattccag attttgcttg aagatattct tgaaaaggac
     2461 tatgatttga tttgtttcca agaaatcaat caggagatga cctcgtcaga ggtggaggtt
     2521 aatgaccttt atcaagcttt gccagcagct gagcctattc accaagacca ttatgttaga
     2581 ctcttggttg aaaagttgtt agagcaaggg aaaaattact actggacctg ggcttataac
     2641 catatcggct atgaccgcta ccacgaaggt gtggctatct tgtctaaaac acctattgaa
     2701 gccagagaaa ttttggtttc agatgtggat gatccaacag actatcatac tcgccgtgtt
     2761 gccctagctg aaactttagt cgatggcaag gagctagcag ttgccagtgt tcatctctct
     2821 tggtgggata aaggtttcca agaagaatgg gcacgatttg aggctgtctt gaaaaaattg
     2881 aacaagccac ttttactagc tggagatttt aacaacccag caggtcagga aggttaccaa
     2941 gctattttag ctagtccatt aggcttacaa gacgcatttg aagttgctca agagaaaagt
     3001 ggtagctata ctgttccgcc tgaaattgat ggctggaaag ggaacactga accccttcga
     3061 atcgattatg tctttactac caaagagtta gcggtggaaa atttacatgt cgtatttgat
     3121 ggtaacaaga gtccacaagt gagtgatcac tatggcttga atgctatgtt aaactggaaa
     3181 taataactga aaagaggttg gaactataaa attccagcct tttcttacta gagaagctac
     3241 tggaaatagc ctaaataagt gagactactg taatggaata aaatatggta taattgataa
     3301 gatagataga atcgaggatg ttatgtcatt tacgaaattt caatttaaaa actatattag
     3361 agaagccttg aaggagttaa aatttacaac tccaacagag gtgcaagata agttgattcc
     3421 tattgttttg gcaggtcgtg acctagtagg agaatcaaaa acaggttcag gtaagactca
     3481 tactttcttg ttaccgattt tccagcaatt agatgaagct agcgatagtg tacaagcagt
     3541 gattactgca ccgagtcgtg agttggctac tcaaatttac caagtagcgc gtcagatttc
     3601 agctcactca gatgtcgaag ttcgtgtggt taattatgtg ggtggtacgg ataaggctcg
     3661 ccagattgag aaattggcaa gcaatcagcc tcatattgtt attggaacac caggccgtat
     3721 ctacgacttg gttaaatctg gtgatttagc tattcataaa gccaagacat ttgttgttga
     3781 tgaagcagat atgaccttgg atatgggatt cttggaaatt gttgataaga ttgctggcag
     3841 tcttccaaaa gacttgcaat tcatggtctt ctcagcgact atcccacaaa aactgcaacc
     3901 attcttgaaa aaatacttat caaatcctgt tatggagaaa attaagacta aaacggttat
     3961 ttctgacacc attgataatt ggttgatttc gaccaaggga catgataaga atgctcaaat
     4021 ttaccaattg actcagttga tgcagccgta tttggcaatg atttttgtta acactaaaac
     4081 gcgtgctgat gaattgcatt catatctgac tgctcaaggc ttgaaggttg caaaaatcca
     4141 tggagatatt gcccctcgtg aacgcaagcg aatcatgaat caggtgcaaa atctggattt
     4201 tgagtatatt gtcgcaacag atttagcagc gcgtgggatt gacattgaag gtgtcagcca
     4261 tgtcatcaat gatgccattc cgcaagactt atcttttttt gttcaccgtg ttggtcgtac
     4321 tggacgaaat ggcctaccag gtacagctat taccctttat cagccaagtg atgactcgga
     4381 tatccgtgag ttggagaaat tgggaatcaa gtttagtcct aagatggtca aagatgggga
     4441 atttcaagat acctatgacc gtgatcgtcg tgccaaccgt gagaaaaaac aagataaact
     4501 tgatatcgaa atgattggtt tggttaaaaa gaaaaagaaa aaagtcaaac cgggttataa
     4561 gaagaaaatt caatgggcgg ttgatgaaaa gcgccgtaaa actaagcgtg ctgaaaatcg
     4621 cgctcgcggt cgtgcagagc gtaaagctaa acgccaaaca ttttaataga aattgttgga
     4681 gtactgagct ccaacttttt tatttatgag aacgaactat ctaaaccgaa acactacatt
     4741 aaagactgca aattgcgatt aaaaatggta taatgataaa gttatatagt cccgataaga
     4801 tggtaggtat ttattacgaa gagttttcct atcagtagtt tgcaattcgc aaagaatgac
     4861 ggcttcgagg tcgctcgtct ttctacggtt gccgccatac tgcggtcacc ctatgcgcct
     4921 tactagcttc agaaataaaa aagtatcgtt tttatttctg aagctagtaa ctcttagata
     4981 atggaaattc ctatcagtac tttgtaactc tataacaata ttttttaagg ggggacattt
     5041 ttatgtcaga acgtaaatta ttcacgtctg aatctgtatc tgaggggcat ccggataaga
     5101 ttgcagacca aatttcagat gcgattttgg atgctatttt agcaaaggat ccagaggcgc
     5161 acgttgctgc tgaaacagct gtatatactg gttctgtcca cgtttttggt gaaatttcta
     5221 caaatgccta tgtggatatt aaccgtgtgg ttcgtgatac cattgcagag attggttata
     5281 ccaatacaga atatggattt tctgctgaga cggtgggagt acacccatct ttggtggaac
     5341 aatctcctga catcgctcaa ggtgttaacg aagccttgga ggttcgtgga aatgctgatc
     5401 aagatccact ggacttgatt ggagcaggtg accaagggct catgtttgga tttgcagtag
     5461 atgaaacaga agagctcatg ccattgccaa ttgcactcag tcataaattg gttcgtcgtc
     5521 tggcagaact tcgtaagtct ggagaaatta gctatctccg tccagatgca aaatcacaag
     5581 ttacagttga gtacgatgaa aatgaccgtc cggtacgtgt agatacagtc gttatttcta
     5641 ctcagcatga tccagaggcc actaatgaac aaatccatca agatgtgatt gacaaggtca
     5701 tcaaagaagt tattccatct tcttatcttg atgataagac aaaattcttt atcaatccga
     5761 caggtcgttt tgtaatcggt ggtcctcaag gggactcagg tttgactggt cgtaagatta
     5821 ttgtagatac ttatggtggc tactctcgtc atggtggtgg tgccttctct ggtaaagatg
     5881 cgactaaggt ggatcgttca gcctcttatg cggctcgcta tattgccaag aatatcgttg
     5941 cagcaggcct tgctaagaag gcagaagtgc agttggccta tgctatcggt gttgcgcaac
     6001 ctgtttctgt tcgtatcgat actttcggta caggaacagt agctgaaagt caacttgaaa
     6061 aagcggctcg tcaaatcttt gaccttcgcc ctgcagggat tatccaaatg ctggacctca
     6121 agcgtccaat ttaccgtcaa acatcggctt acggtcacat gggacgtaca gatattgatc
     6181 ttccatggga acgtttggat aaggtagatg ctttgaaaga agcagtaaaa taagatttta
     6241 agaggggaac gtcctctctt ttttatagtt tttaactata ctgggatact gttctgaaaa
     6301 tccattttgc gaaagtagag atttacatgt ataatggatt gaaactagaa tagtacacct
     6361 caacttctaa aacattgtta gaaatcgatt tgactgtcct gatcgatttc tcctgttctt
     6421 gtttcatttt actatatttc tttaaaaatg ataaaggttg agatttctcc tcgtaataga
     6481 taatcatggg gatatttcaa tccaaagttt tattcgttat cacttgacta ttgcaaggtt
     6541 ttctagagca acagagtcat ggaatggact catggttgag atttctcctt gttgcttgga
     6601 cttcattcaa aagtctgtta cccaagcctt gttcaaactt ccaatacact agctgtttcc
     6661 atagcatgac ttctgtacta gactttcttt tccgaataaa tagatagaac cacagaatct
     6721 agtaaaccta gaattaaaat tatggtataa tattagcaat aaaagaaatc tggaggatta
     6781 gaatcatggt atcaacgaaa acacaaattg ctggttttga gtttgacaat tgcttgatga
     6841 atgcagcagg tgtggcttgt atgacgatag aggagttaga agaggtcaaa aactcagcgg
     6901 caggaacctt tgttactaag acagcgacct tggacttccg tcaggggaat cctgagccac
     6961 gctaccaaga tgttccactt ggttccatca actctatggg cttgccaaat aatggcttag
     7021 actattattt ggattatctt ttagatttgc aggaaaaaga gtcgaaccga actttcttct
     7081 tatctctggt cggcatgtct ccagaggaaa cccatactat tttgaaaaaa gtccaagaga
     7141 gtgattttcg tggtctgact gagctaaatc tttcctgtcc aaatgttcca ggtaaacctc
     7201 agattgccta tgattttgag acaacagacc ggattttggc agaagtgttt gcttacttca
     7261 ccaaacctct tggaattaaa ttgccacctt attttgatat tgttcacttt gaccaagcgg
     7321 cagctatttt caacaaatat ccgctcaagt ttgtcaactg tgttaactct atcggaaacg
     7381 gcctctatat agaagacgaa tctgtcgtta ttcggcctaa gaatggtttt ggtggaattg
     7441 gtggagaata catcaaaccg actgctctag ccaatgttca cgccttttat caacgtttaa
     7501 atcctcaaat ccaaattatc ggaacaggtg gcgttctgac tggtcgagat gcctttgaac
     7561 acatcctctg tggagcaagt atggtgcagg tgggaacgac ccttcacaaa gaaggcgtca
     7621 gtgcttttga ccgcattacc aatgaactga aagcaatcat ggtggaaaaa ggctacgaga
     7681 gcttagaaga tttccgtggg aaattgcgct atattgacta aattaaatcg aaaaatctga
     7741 agaaaggaga gacgatgcta gccattgaag aaagtcagaa gttgacttta tcaaatttac
     7801 cgagcctgag cctatttaca gggacagatc agggtcagtt tgaagtgatg aagagtcaag
     7861 tgttgaaaca gattgggtat gattctgctg acctcaactt tgcctacttt gatatgaaag
     7921 aagtagttta caaggatgtg gaactggagt tggtcagcct tcctttcttt gcggatgaga
     7981 aaatcgtgat attagattat tttatggata tcacgactgc taagaaacgc tttttgacag
     8041 atgatgagct taagtcattt gaggaatacc ttgacaattc ttctccaaca accaagttga
     8101 taatctttgc agaaggaaag ctggatagca aaagacggtt agtcaaatta cttaagcgtg
     8161 atgccaaggt cttcgatgca gtagaagtaa aagaacaaga attgcgccag tatttccaaa
     8221 agtggagtca gaaacaaggt ctgcagttta ccaatcattc ttttgaaaat ctcctcatca
     8281 agtcggggtt tcaatttagc gaaatccaga aaaatcttct ctttttacag tcctataagg
     8341 cgaattctgt tattgaggaa gaggatattg ttaacgcaat tcccaagacc ttgcaggaca
     8401 atatttttga tttaactcag tttattctga ctaaaaagat ggatcaggcg cgcgatttgg
     8461 tgagagactt gaccttgcaa ggggaagatg aaatcaaact gattgcagtc atgctgggac
     8521 aatttcggac ttttactcag gtgaagattt tggcggagtc tggccaaaca gaatcgcaga
     8581 ttgcaagtag tttaggtagt tatctgggac gtaacccaaa tccttatcaa atcaagtttg
     8641 cattaagaga ttcgagagga ctttctttga gctttctgaa gcaagctatt tcctatttga
     8701 ttgagacaga ctatcagatt aagacaggtc tttatgaaaa aggtttcctt tttgaaaagg
     8761 cactcttaca gattgctagt caggtcaatt gacatttgtt gaaactacaa cccgcgtcag
     8821 gattatcttg tcgtgggttt cttgctgatt tgcttatttt attagaaagt atgtcagtga
     8881 tttccttatc tataagcaaa aaacttgaaa aaagtgaatg tttgcgttat cattttcata
     8941 tagaaagaaa aagaggtatt acagatggct attatcttac cagaacttcc atatgcatac
     9001 gacgctttgg aaccatacat cgatgcggaa acaatgcatt tgcaccatga caaacaccat
     9061 caaacttatg tcaacaatgc caatgcagct ttagaaaaac accctgaaat cggtgaagac
     9121 cttgaagcct tgcttgctga tgtagaatct atcccagctg atatccgtca agcacttatc
     9181 aacaatggtg gcggacactt gaaccacgct cttttctggg aattgatgac tcccgagaaa
     9241 acagctcctt cagcagaact ggcagcagca atcgatgcaa catttggttc atttgaagaa
     9301 ttccaagcag ccttcactgc agcagcaaca actcgttttg gttcaggttg ggcatggttg
     9361 gttgtcaaca aagaagggaa acttgaagtg acttcaacag caaaccaaga cacaccaatc
     9421 tcagaaggta aaaaaccaat cttgggcttg gacgtttggg aacatgctta ctacgtgaaa
     9481 taccgcaacg tgcgtcctga ctacatcaaa gctttctttt cagtaatcaa ctggaataaa
     9541 gtagatgaat tgtacgcagc tgctaaataa tgatagttgg agggaagaat tgttcttctc
     9601 tttttaggtt ataagattct ggtctgacaa aatcgtcaga cttttttcat ttttatgaga
     9661 aacgtgctaa ctgctagaaa ttatggtaga ataaagtatt aagtaatcgt ggaaaaagga
     9721 tatctatgcg aaaagaaatt gcacctgaat tatacaacta taacaagttt cctggtccgg
     9781 agttccattt acacggggat aaggtcgaaa cggaagggat agctttttcc ttggttgaaa
     9841 atatcaagga tgcctttgat gtgacgactt ttaatcagcg tttttcagaa gtcttaacca
     9901 agtttgatta tatcgtgggg gactggagca acgaacagct tcgcctacga ggtttttaca
     9961 aggatgaccg aacagaagaa aaacttgaaa aaatcagtcg tttacaagac taccttttag
    10021 agtattgtag ttatggttgt gcctattttg tcttagaaaa tgaagcccct aagcgagcat
    10081 catttgacaa gaaaatgcgt aagacggaag aagaaacacc ttctagaaaa ggaaagaaac
    10141 cgactcaaac taaacgaaag tcgaatgcag ataagaaaaa tagacgtcgt cagaaagacc
    10201 agcattctca gaaagaggac aaggaacaac gtcattttgt cattcgtcag aagtgagtag
    10261 agaataagga gaagaaatga aaccctcaat tcatagtttg gcacatcaaa ccatgcagga
    10321 atgggtattg gagcagggag aaaagaaatt ccgtgcagat caaatctggg aatggctcta
    10381 ccgtaaacgt gtgcagtcat ttgaagaaat gaccaacctt tccaaggatt tgattgctaa
    10441 gctcaatgac cagtttgtgg tcaatccctt gaaacaaggt attgtgcaag agtctgctga
    10501 tggtacagtc aaatatctct ttgaattgcc tgacggtatg ttaattgaga ctgtactcat
    10561 gtgtcaacac tatggtttat ctgtctgtgt gaccactcag gtcggctgta atatcggttg
    10621 taccttctgt gcctctggtt tgattaagaa gcagcgtgac ctcaataacg gtgaaatcgt
    10681 agcgcaaatc atgctggttc agaaatactt tgatgagcgt ggtcaggatg aacgcatcag
    10741 ccatatcgtt gtcatgggaa tcggtgagcc ctttgataat tacaacaatg tcttgaattt
    10801 ctttcgtact atcaatgatg ataaaggaat ggctatcggt gctcgtcaca tcacggtttc
    10861 aatctcaggt ttggctcata aaattcgtga ctttgctgat gaaggggttc aggtcaatct
    10921 tgccgtgtct cttcacgcac ccaacaatga acttcgttca agtatcatga agattaaccg
    10981 tgcctttcc
//
LOCUS       DAVBCB010000064        10764 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVBCB010000064 DAVBCB010000000
VERSION     DAVBCB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10764
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.064"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..233
                     /locus_tag="VQU28_001830"
     CDS             51..233
                     /locus_tag="VQU28_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HET2477133.1"
                     /translation="MKSQKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            533..1372
                     /locus_tag="VQU28_001831"
     CDS             533..1372
                     /locus_tag="VQU28_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HET2477134.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            1390..3228
                     /locus_tag="VQU28_001832"
     CDS             1390..3228
                     /locus_tag="VQU28_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HET2477135.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPDVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTPAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     NLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            3241..4656
                     /locus_tag="VQU28_001833"
     CDS             3241..4656
                     /locus_tag="VQU28_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HET2477136.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEN
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSAIPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            5248..6294
                     /gene="pheS"
                     /locus_tag="VQU28_001834"
     CDS             5248..6294
                     /gene="pheS"
                     /locus_tag="VQU28_001834"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HET2477137.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            6294..6803
                     /locus_tag="VQU28_001835"
     CDS             6294..6803
                     /locus_tag="VQU28_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2477138.1"
                     /translation="MIRKVEMADVEVLAKIAKQAFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            6880..9285
                     /locus_tag="VQU28_001836"
     CDS             6880..9285
                     /locus_tag="VQU28_001836"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HET2477139.1"
                     /translation="MLVSYKWLKELVGIDVPSQELAEKMSTTGIEVEGVESPAAGLSK
                     IVVGEILSCEDVPETHLHVCQVNVGEEEERQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKKFADGIQILPENAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMRGVAHEVAAIYDKAVNFKEFTLTETNEAATDALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SHVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYVLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLEVTYTATAEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSVIEAALQPAAPFVEITKFPAVSRDVALLLKAAVTHQEVIDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEKKVNA
                     EVR"
     gene            complement(9458..10459)
                     /locus_tag="VQU28_001837"
     CDS             complement(9458..10459)
                     /locus_tag="VQU28_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HET2477140.1"
                     /translation="MIKTFLSALSVILFVIPIITYSFFPSSNLNIWLSTRPILAQIYA
                     FPLATATMAAILSFVFFSLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIADDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
     gene            10615..>10764
                     /locus_tag="VQU28_001838"
     CDS             10615..>10764
                     /locus_tag="VQU28_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2477141.1"
                     /translation="MQDNYTTKGKHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKR"
BASE COUNT         3449 a         1734 c         2287 g         3294 t
ORIGIN      
        1 accccaaaag ttagattttt tctgtctaac ttttggggtg tagttcacag ttgaagagcc
       61 agaaaattaa agtgtctttt actcaatcaa aaatagtaac gcctattctt ttggtgaata
      121 agactattgt ttggtatggt gcaatgccat tattagggaa ggtagatgag atgaccatat
      181 tacgtttgga atcagctagt atagtttctg aactagtggc aggtttacga tagagaaaat
      241 ttttaaaaat ttctatgtat gattttcatt tctttagtga gactgttgcc attatcacat
      301 tcgaatcaca caaaataaaa aaatttctac aagtacttga caaatagatt gaaatatcat
      361 aaaatgaaaa tggttacaga gttattaatt atttaagctt catgtcacca ttaaaaattg
      421 aaataaaagg atgttatcac taatacaagt gagcaggaac ctatttaatc acatcggaag
      481 aagtttcttg atgtttttaa gtaggttcct tttattttaa aagggaaatt ttatgatcat
      541 aaaacgaata ctaaaccaca atgccgtaat tgcgcaaagt aaaaaagata tcgatattct
      601 tctttttgga aggggcatag cttttggaag aaaaactgga gataaagtaa atccaattga
      661 tattgagaaa agtttttttc tcaaaaatag agataatatg acccgtttta cagagatgtt
      721 tattaacgtt cctttggagt tggtgtacat caccgaaaaa ataattaacc taggtaaaat
      781 aacattgggt aataattttg atgaaattat ctatattaat ttaacggatc atatttcttc
      841 gagcatagaa cgttataaag aagggattat tatttcgaat cccctacgct gggaaatatc
      901 gaaatattat aaagaagaat ttgaacttgg gaaaagggct ttacaaataa taaaaaaaga
      961 gttaggtatt gaacttccaa ttgacgaagc tgcattcata gcgctacatt ttgttaatgc
     1021 taatttagaa aataattttc aagagtcgta taaaatcact gaaataatta tgggaattga
     1081 gaaaatcatt caagatttct attgtactga gtttaaccaa gattctattg attattatag
     1141 attcataact catataaaat tatttgctca tcgcttggtt gaaaatacaa cttattgtga
     1201 cgatgatgat gaagacttgt tagcattaat gaaaaataaa tatcctagag aatatgaatg
     1261 tggtgaacag gtggcaatgt ttatacaaac tgagtacaat tatttgctga cttctagtga
     1321 attagtttat ttgatggctc atattcgtcg cttaacaaaa aatttagatt aatagaatag
     1381 gagaatacca tgagttataa agatacggta caaaaaatcc tcgatgtaat tggaggtgaa
     1441 aaaaatgtca atagagttac ccattgtgta acacgtttaa gattagaatt aaaagatgaa
     1501 aatttagtca atgatgatga tgtgaagaag ataccagatg taataggtat tatgaaaaag
     1561 aatggacaat atcaaattat acttggtaat gatgtagcta attattataa agaattcgtt
     1621 aaacttggca attttgaatc cgattcagtt gttcaagggc acaaagggaa tattttagaa
     1681 agaatcattg agtatatcgc tggttccatg actccaatca ttccagcaat gttaggggga
     1741 ggtatgttga aagtcttggt aatcatttta ccaatgcttg gtatattgca atcagattct
     1801 cagactattg cttttttgac attttttggg gatgctccat attatttctt accgctgtta
     1861 ttagcttatt ctgcatcaca aaaattaaaa gtaacatcta cattagctat gtctgtagca
     1921 ggtgtacttc tccatccaaa ttttgttcaa atggtgcaat cagggaatcc tcttagttta
     1981 tttggtgcac ctgtgacacc agctagttat ggttcatcag tcgttccaat tcttattatg
     2041 gtttggttga tgaaatatat tgaaaaaata attgctaaat taacaccagc tattactaag
     2101 agttttttgc aacctacgct agtattatta gtatcaagct gtattgcctt agttgtagtc
     2161 ggacctattg gagtaattgt tggtgaagga ttatcaaatc tagttgggca aatgtatggt
     2221 gtagctggat ggcttacatt agctattctt ggtgctatta tgccatttat tgttatgact
     2281 ggaatgcatt gggcttttgc acctattttt ttggcggcat ctattgctac tccagacgta
     2341 ttaattcttc cagcaatgtt agggtcaaac ttagctcaag gggctgcttc gatggctgtt
     2401 gcattaaaga gtaaaaataa taatacaaaa caaattgctt ttgcagcagg tttctcagcc
     2461 ttacttgcag ggattaccga acctgcatta tatggtgtga ctttaaaata taaaaaaccg
     2521 ctttatgcag ctatgattgg tggtggatta gcgggattat ttgcaggtct tactagtgtt
     2581 aaagcatatc tatttgctgt cccatctttg atagcgttgc ctcaatttat ttattctgat
     2641 gtgccatcaa atattgtaaa tgctttaatt gtggcggtca tttcggttgt tattaccttt
     2701 gtattagctt atatatttgg aatcgatgaa gaagagagtt ctagcaattt agaagttaaa
     2761 gctggagttt caaataaaaa aatgatattt tctcctatat caggagaaat cattccgtta
     2821 agcgatgtcc aggataaaac attttcagat aaattaattg gagacggagt agcgattatc
     2881 ccaagtgaag gtaaggttta tgcaccattt gatgggaaaa ttacaaatat ttttccgact
     2941 aagcacgcaa ttggattgaa gagtgatgag ggtgttgagt tactaattca tattggatta
     3001 gatactgttg agctaaaagg tcaaggtttt attagtcatg tagaagaagg agacagagtt
     3061 ttcaaaaatc agttgatttt tgaaatgaac ttgaatttaa tcaagactaa aggctacgaa
     3121 acagttacac cagtaattgt aacgaatacc aatgattttc tagatgtatt agtattacct
     3181 aataatcaga caatcgagca ttctaaggaa ttactggtaa tattataatg gaggaaagtt
     3241 atgaccattt ttccagatga ttttctttgg ggtggagcgg ttgcagctaa tcaagtagag
     3301 ggagcatata atgaagatgg taagggctta tcagttcagg atgtgttacc caaaggtgga
     3361 ttaggagaag caacagaaaa tcctacagaa gataacttaa aattgatagg tattgatttt
     3421 tatcataaat ataaggaaga tatatccttg ttttctgaaa tgggctttaa tgtttttcgt
     3481 acttctattg catggagtag aatttttcca aaaggagatg aggaagaacc taacgaagct
     3541 ggtttgaaat attatgatga attatttgat gaactacatg ctcacgggat agaaccactt
     3601 gtaactcttt cacactatga gactccatta tatttagcaa gaaaatatca tggatggatt
     3661 gataggagaa tgattcattt ttatgaaaaa tttgctcgaa cagttttaga aaggtataaa
     3721 gataaagtta aatattggct tacatttaac gaagtaaact ctgttttgga attaccgttt
     3781 actagtggag gaatagatat acctaaggag aatctttcga aacaagaatt atatcaagct
     3841 atacatcatg aattagtcgc ctcgagtttg gttacaaaaa ttgctcgtga gattaattca
     3901 gagtttaagg tggggtgtat ggtattagct atgccagctt atccaatgac tccaaatcca
     3961 aaagatgtat gggcgactca tgagtatgag aatctaaatt atctattttc agatgtgcat
     4021 gttagaggtt attatccgaa ttatgcaaaa agatatttta aggaaaatga cattaacata
     4081 gagtttgcag ctgaagatgc agagttatta aaaaattata ctgtagattt tttatccttt
     4141 agctattaca tgagtgtgac tcaatctgct attccaacac agtataattc aggagaaggg
     4201 aatattattg gtggtttagt aaatccttat ttagagtctt cagagtgggg atggcaaatt
     4261 gatccaattg gactacgtat aattttaaat agatattatg accgttatca aatcccatta
     4321 tttatagtag aaaatggatt aggtgctaaa gatcaactaa taaaagatga acttaataac
     4381 ttaaccgtcc aagatgatta tagaattcag tatatgaaag aacatttatt gcaagttgct
     4441 gaagctctac aggatggtgt tgaaattatg ggctatacgt cttggggatg tattgactgt
     4501 gtttctatgt ccactgcaca actttctaaa aggtacggtc tcatttatgt tgatcgaaat
     4561 gatgatggaa atggcacatt caatcgatat aagaaaatgt cctttacttg gtataaagga
     4621 gtgattgaat caaatggaga atccttattc aaatagtttg tttattgatg attagatatt
     4681 atcacggtta aggcaacatc tcattaaata taaaataaag aggtttggaa gacatcaaat
     4741 tctaaacctc tttattttat agggggatta attaatttaa ttttgttaca gagagccgat
     4801 tataaatagt taacttagga tagattggtt atatatagta gactgaatct aaaatagtac
     4861 gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga tttgttttca
     4921 tcttatttca atccgctata aagttagata agaataagct aacaagcaga agtaagatag
     4981 gtcttgcaag aatggtaaaa atctgataaa ctaagtagta attgaatagg aatctgaaag
     5041 actagtagcc caagggaagt atatcaggga ggagagccgt gactgcaagc tctctatatg
     5101 aaagctgggt gaattcactt gcgcatggat ttagaaatga aggtctgact tgtcagatga
     5161 aaacggatgg taccgcgtgt caacgctccg agtggagttt ttggcatgtg gttttctttt
     5221 tatctacgag agactgatgg aggaaatatg tcaactattg aagaacaatt aaaagcgctt
     5281 cgcgaagaaa cgctggctag cttgaagcag attactgctg gaaatgaaaa agagatgcaa
     5341 gatttgcgtg tctctgtcct tggtaaaaag ggttcgctca ctgaaatcct caaagggatg
     5401 aaagatgttt ctgctgagat gcgtccaatc atcgggaaac acgtcaatga agctcgtgat
     5461 gtcttgactg ctgcttttga agaaacagct aagctcttgg aagaaaagaa agtcgcggct
     5521 caactggcta gcgagagtat cgatgtgacg cttccaggtc gtccagttgc gactggtcac
     5581 cgtcacgttt tgacacaaac cagtgaagaa atcgaagata tcttcatcgg tatgggttat
     5641 caagtcgtgg atggttttga agtggagcaa gactactata actttgaacg tatgaacctt
     5701 ccaaaagacc acccagctcg tgatatgcag gatactttct atatcactga agaaatcttg
     5761 ctccgtaccc acacgtctcc agttcaggcg cgtgctatgg atgcccatga tttttctaaa
     5821 ggtcctttga agatgatctc gccagggcgt gtcttccgtc gcgatacgga cgatgcgacc
     5881 cacagtcacc aattccacca aatcgaaggc ttggtagttg ggaaaaatat ctctatggct
     5941 gatcttcaag gaacgcttca gttgattgtc caaaaaatgt ttggtgaaga gcgtcaaatt
     6001 cgtttgcgtc catcttactt cccattcaca gagccatctg ttgaggtgga tgtttcttgc
     6061 ttcaagtgtg gtggagaagg ctgtaatgta tgtaagaaaa caggttggat cgaaattatg
     6121 ggggccggta tggttcaccc acgtgtcctt gaaatgagtg gtatcgatgc gactgtatac
     6181 tctggctttg cctttggtct tggacaagag cgtgtagcta tgctccgtta tggaatcaac
     6241 gatatccgtg gattctacca aggagatgtc cgcttctcag aacagtttaa ataatgatta
     6301 gaaaagtaga aatggcagat gttgaggtgt tggctaaaat tgccaaacaa gcctttcgtg
     6361 aaacctttgc gtatgataat acggaagagc agttacagga atactttgaa gaggcttata
     6421 gtctgaaaac tttgtcaact gagttgggaa atcctgactc tgaaacctat ttcattatgc
     6481 atgaggagga gatagctggt tttctcaaag tcaactgggg aagtgctcaa actgagagag
     6541 aattagagga cgcttttgaa attcaacgcc tctatgtgct acaaaaattc caaggatttg
     6601 gactaggtaa gcaactgttt gaattcgcac ttgaacttgc tacaaaaaat agtttttctt
     6661 gggcttggct aggtgtttgg gagcataata caaaagctca agcgttttat aaccgatatg
     6721 gttttgaaaa atttagccaa catcatttta tggttggtca aaaagtagat acggattggt
     6781 tactgagaaa gaaattaagg taagaagaga attcttctat tgaaatgata ggaaaggaaa
     6841 agaaccagtg tggcaatggt cattctggag aactaaatta tgcttgtatc ttataaatgg
     6901 ttaaaagaat tggtgggcat tgatgtgcca tcacaggagt tggctgaaaa aatgtcaacc
     6961 acagggatcg aggtcgaagg tgtcgaatca ccagctgccg gtctttcaaa aattgtcgtc
     7021 ggtgagatct tgtcttgcga agatgtgcca gaaactcacc ttcatgtttg tcaggttaac
     7081 gttggcgaag aagaagagcg tcagatcgtt tgtggtgccc caaatgtgcg tgcaggcatc
     7141 aaggttatgg tggctcttcc gggagctcgt atcgctgaca attacaaaat caaaaaagga
     7201 aaaatccgtg gcttagagtc acttggaatg atctgttcac ttggtgaatt gggtatttct
     7261 gactcagttg tacctaagaa attcgcagat ggtattcaaa ttttgccaga aaatgccgtt
     7321 ccaggtgagg aagtcttttc atatctagac ttggatgatg aaatcatcga actttctatc
     7381 acaccaaacc gtgcagatgc cctttctatg cgtggagtgg ctcacgaagt ggcagccata
     7441 tatgacaagg cagtcaactt taaagaattt actttgacag aaacaaatga agctgcgaca
     7501 gatgcccttt ctgtgggcat tgagacagac aaggcgcctt actatgcagc tcgtatcttg
     7561 gacaatgtga ctatcgcacc aagtccacaa tggttgcaaa accttctcat gaacgaaggc
     7621 atccgtccca tcaataacgt tgtagacgtg acaaactaca tcctgctcta ctttggtcaa
     7681 cctatgcatg cttttgactt ggacacattt gaagggactg acatccgtgt gcgtgaagcg
     7741 cgtgctggtg aaaaattggt gaccttggac ggtgaagaac gtgacttgga cgtgaatgac
     7801 ctagtcatca ctgttgcaga caagccagta gcccttgcag gtgtcatggg tggtcaagca
     7861 acagaaatct ctgaaaaatc tagtcatgtt gtccttgaag ctgctgtttt caatggcaaa
     7921 tctatccgta agacaagtgg tcgcctgaac cttcgttctg agtcatcttc tcgctttgaa
     7981 aaaggaatta atgtggcaac agttaatgaa gcccttgatg cggcagctag cctgattgcg
     8041 gaacttgcag gtgcgacggt gcgtaagggt atcgtttcag cgggtgagct tgatacttca
     8101 gatgtagaag tttcttcaac tcttgctgat gttaaccgtg tcctcggaac tgagctgtct
     8161 tatgctgatg tcgaagacgt cttccgtcgt cttggctttg gtctttctgg aaatgcagac
     8221 agctttacag tcagcgtacc acgtcgtcgt tgggatatca caatcgaagc tgatctcttt
     8281 gaagaaatcg ctcgtatcta tggatatgac cgcttgccaa ccagccttcc aaaagacgat
     8341 ggtacagctg gtgaattgac tgtgacacaa aaactccgcc gtcaagttcg taccattgct
     8401 gaaggagcag gtttgacaga aatcatcacc tatgttctaa caactcctga aaaagcagtt
     8461 gagtttacgg ctcaaccaag taaccttacg gaactcatgt ggccaatgac tgtggatcgt
     8521 tcagtcctcc gtcaaaatat gatttcaggt atccttgata ccgttgccta caacgtggct
     8581 cgtaagaata aaaacttggc cctttacgag attggaaaag tctttgaaca aacaggtaat
     8641 ccaaaagaag aacttccaaa tgaaatcaac agttttgcct ttgccttgac aggcttggtt
     8701 gctgaaaaag atttccaaac agcagcagtt ccagttgatt tcttctatgc taaaggaatc
     8761 ctagaagcgc tctttactcg cttgggacta gaagtgacct atacagcaac agctgagatt
     8821 gccagccttc acccaggacg tacagcagtc atttcactcg gtgaccaagt tcttggtttt
     8881 cttggccaag tgcatccagt cactgctaag gcttacgata ttccagaaac gtatgtggct
     8941 gagctcaacc tttcagtcat cgaagctgcc cttcaacctg ctgctccatt tgtggaaatc
     9001 acgaaattcc cagcagtcag ccgtgacgtt gcccttctcc tcaaggcagc agtaactcac
     9061 caagaagtta tagatgctat ccaagctgcc ggcgtgaaac gtttgacaga tatcaaactc
     9121 tttgacgttt tctcaggtga gaaattggga cttggtatga agtcaatggc ttatagcttg
     9181 accttccaaa atccagaaga cagcttaacg gacgaagaag tcgcacgcta catggaaaaa
     9241 atccaagcat cgcttgaaaa aaaagtgaac gcagaagtgc gttaaacgat agtaggatac
     9301 aaattccccc tctagtttat gaacactaga ggggtgattt tatataatac tcaatgaaaa
     9361 tcaaaaagca aactaggaag ctagccgtaa gctgtacttg agtactgtaa ggcgaggctg
     9421 acgtggtttg aagagattgt cgaagagtat aaaataatta aaatgtgatt tctgtaaaaa
     9481 tacatctatg atcagagttt tgaaaactta caatgtctaa atctttaaca tagtagtggt
     9541 ttttaggcaa taaaatatga tctattgttg cattaaaaag ttttggactt tggctattcc
     9601 aagttcctct ttcaaaaggt ggtagtacat ttaatgcgtc cctatgagag cttatttttg
     9661 caagtgctcc atgacgcata gttgcattaa aatcatctgc aataatagcc tttggatatt
     9721 ttgaagccaa ttgattatga atgatgttta agtcttgctt ccagatttcc attaaacctg
     9781 gcagaggagg agcagtatgc aaggcaatga tatctggtat attttgtttt ctcgaatgta
     9841 atacaattgt cccgaaccgt gttgtatgaa aagttttagc ttctgtatag aaaccataac
     9901 ttttcttgac aatcacagtc acaggagcta ttccactatt ggtaggtgga gaagtgaaaa
     9961 tatcatagtt ggccatagaa agtccaactt gatgaaacaa tagtttgatt ctctggtttt
    10021 cttgctcacc tctgatattg gtagctagtt cagggaatat agccatatcg gcgtcaaaat
    10081 ggctaaaaat tcgctcaata tgttgtgctt ctatttgatt agcgacgttc caagttacta
    10141 attttaaggt tttagtctta tttgatgcag aagaaagggt tttatctgtt ccgaataata
    10201 gtaatatgag cgatagtaag agcaaaatgc cagagtaaaa ccgtatttgt ttatttttct
    10261 tgtaaaaaga tagggaaaaa aatacgaaac ttaaaatagc agccatagtt gcagtagcta
    10321 aggggaaggc ataaatctgt gccaagatag gtctggtaga tagccaaata ttaagattag
    10381 aagatggaaa aaaagaataa gttatgatag ggataacaaa gagaatgacc gaaagggcag
    10441 agagaaatgt tttaatcatt gtgtgagtac ctcaatttgc aatggttgct cggagctaat
    10501 gtgactttgt gactttactt gattgatatt ctttttcttg tcttcctcat agattaagta
    10561 caacccaagt aaaaaaagtc caatttaaga ttgaaatgat taaaaggcac agaaatgcaa
    10621 gacaattata ctacaaaagg caaacatttg acaatcgata gccgtcgctt aatcgaaaga
    10681 tggaaaaaag aagggaaatc aaatagagaa attgcctctc tgcttggaaa agttcctcaa
    10741 actatccaca ctgaaatcaa gcgt
//
LOCUS       DAVBCB010000065        10264 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVBCB010000065 DAVBCB010000000
VERSION     DAVBCB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.065"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(302..1282)
                     /gene="dusB"
                     /locus_tag="VQU28_001839"
     CDS             complement(302..1282)
                     /gene="dusB"
                     /locus_tag="VQU28_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HET2477142.1"
                     /translation="MTNLNTPFMIGNIEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1269..2141)
                     /gene="hslO"
                     /locus_tag="VQU28_001840"
     CDS             complement(1269..2141)
                     /gene="hslO"
                     /locus_tag="VQU28_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HET2477143.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQERHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2306..3733
                     /locus_tag="VQU28_001841"
     CDS             2306..3733
                     /locus_tag="VQU28_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HET2477144.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLDSAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSAGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3892..5400
                     /gene="glpK"
                     /locus_tag="VQU28_001842"
     CDS             3892..5400
                     /gene="glpK"
                     /locus_tag="VQU28_001842"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HET2477145.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5442..7268
                     /gene="glpO"
                     /locus_tag="VQU28_001843"
     CDS             5442..7268
                     /gene="glpO"
                     /locus_tag="VQU28_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HET2477146.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKHLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPILDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7338..8042
                     /locus_tag="VQU28_001844"
     CDS             7338..8042
                     /locus_tag="VQU28_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2477147.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGGLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8339..8542
                     /locus_tag="VQU28_001845"
     CDS             8339..8542
                     /locus_tag="VQU28_001845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477148.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(8629..9216)
                     /locus_tag="VQU28_001846"
     CDS             complement(8629..9216)
                     /locus_tag="VQU28_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477149.1"
                     /translation="MFVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNILSDEVLE
                     VVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVNTIHGNKQ
                     ANTFANFMTGAGISYMIGRIGTLLGGPWGAIIGGAYFGIQAYQSYLDSQSPYPYYITS
                     TYIHVAQKKWKFITEYYRNSNYTGYVKTVTTYVNF"
BASE COUNT         2982 a         2033 c         2344 g         2905 t
ORIGIN      
        1 tcaggtgccc ttgcagtcaa cacaactgta gatagctata gagtcaatgc caatggtgaa
       61 tgggtaaact aaacctaata taactagtta atactgactt cctgtaagaa ctctttaaag
      121 tattccctac aaataccata tcctttcagt agataatata cccttgtagg aagtttagat
      181 aaaaaaataa ctctgtaatc tctagccgga tttatagcgc tagagactac ggagtttttt
      241 tgatgaggaa agaatggcgg cattcaagag gctctttaag agagttacgg gttttaaact
      301 attaagcctt ctccaattgc aagagggctt caatctctgc cagggtgcta gcttgcgaaa
      361 tggctccacg gagtttggca gcgccagatg ttccacggag atagtgagga gcgaggccgc
      421 ggaattcacg aactgcgacg ttttctcctt tgaggttaat caatcgtttc aagtgttcgt
      481 aggcgatctt catcttgtct tcaaaggtca aatcaggtag gatttctcct gtttcaaagt
      541 aatggttgat ttggttgaag aggtaaggat ttcccatggc agctcggcca atcatgactg
      601 cgtcagcacc aacttcttcg atgcgttgct tggcttcttg gacagtacgg atatcaccgt
      661 tggcgatgaa tggaatcttg gttagagctt gggcaacctt gtaaagggtc tcaaggtctg
      721 cgtggccagt atacatttgt tcacgggtac ggccatgcat ggcgagggca gaaacacctg
      781 cagcctcagc agcgagggca ttttctactg caagagatgg gtccgcccat ccggtacgca
      841 ttttgacagt aagtgggata tcaaggacag actggacctt gttgatgata gagtagatct
      901 tgtcaggatc cttgagccac atagctccag cttcgttctt cacgattttg ttgacagggc
      961 agcccatgtt gatatcgacg atatcggtct tggtgttttc ttggatgaat tctgctgcgc
     1021 gtgctaggct gtcttcatcg ctaccaaaaa gttggataga gacagggttt tcgccctcat
     1081 cgatatgaag catgtgcagg gttttttcgt tgttgtattg gattcccttg tcagagacca
     1141 tttccattac aacgagtcca gctccgagct cttttgcgat ggtacgaaag gctgagttgg
     1201 tcacgccagc cataggcgct aaaacggtac gattgggaat ctcaatattg ccaatcataa
     1261 aaggtgtatt aagatttgtc acgaatgagt tcctccaggt ccttttcatc aaagttgtaa
     1321 gtagtttggc agaattgaca agtgatttct gccccgtggt cttcctcttt catttcctgt
     1381 aagtctgagc ttggaaggct ggcaagagcg ttcataaagc gttcatggct acagtcacat
     1441 tggaaacgga tttcttcttc agaaagacgc ttgtaggctt cgtccccgta gatagccttg
     1501 aggagggctt cgatatggtc gtcgctttcg agaagagtag agatagctgg catttcttgg
     1561 atgcgttttt caaagcgagc aatctcttct ttcttggctc ctggcaagac ttgaactagg
     1621 aaaccacctg caaccttgac cttgtcttcc tcgtccaaaa ggacattgag gccgaccgct
     1681 gaaggcgttt gttggctttc agtaaggtaa aaggcaaggt cttcaccgat ttctccagag
     1741 atgaggggag ttatagagtt gtaaggattt ccagcaccgt agtctgtgat aacgaggaat
     1801 tgaccatttc caacaaaagg tccgactagg acttcaccag tcgcagtctt tttgatgtca
     1861 acaccaggat tttgaacata gcctttgacg ttccccttgg tatcagcgac ggtgataata
     1921 gcacctagag agctagagcc caacacctta actgtaagtt tggtatttcc tttttcattg
     1981 gctgcgagaa tctggctagc gataagagtt cgaccaagcg ctacagttga gctagcttgg
     2041 gtttgatgtc tttcttgagc agtgcggacg gtttccgtgc tatcaaggac aaaagcacga
     2101 aaggctccgc tttctgatat agttttaata attttatcca tagctactat tttagcataa
     2161 aaatgcccaa agggggagcc gtgtgtttac tgattttcag gataatggac caggaaatca
     2221 gcatgaaaat aaaaagagaa acagattatt tcagcatttg taagatttat gctatgctta
     2281 aggtagaaaa tgaaaggggt aacaaatgta tttaggagat ttgatggaga aagccgagtg
     2341 tggtcaattt tcaatccttt cctttctatt acaagagtct cagacgaccg tcaaggctgt
     2401 aatggaagaa acaggatttt caaaagcaac cctaaccaaa tatgtcaccc tgctcaatga
     2461 caaggctttg gatagtggct tagaactgac tattcactca gaagatgaaa atctgcgtct
     2521 gtctatcggt gcagctacca aggggagaga tattcggagc ttgtttttgg atagtgctgt
     2581 taaataccag attttggttt atcttctcta ccaccaacag tttttagccc atcagctggc
     2641 tcaagaattg gtgattagcg aggctacgct tggtcgtcac ttggctggtt taaatcagat
     2701 tttgtcagaa tttgatttat ccatccaaaa tggacgctgg agaggtccag agcatcagat
     2761 tcgctatttc tatttctgtc ttttccgaaa ggtctggtcg agtcaggaat gggaaggtca
     2821 catgcagaaa ccagagagaa aacaggagat tgccaattta gaggaaatct gcggtgcaag
     2881 tttgtctgcg gggcagaaat tggacttggt tctctgggct cacatcagtc aacaacgtct
     2941 tcgggtcaat gcttgtcagt ttcaagtcat agaagagaaa atgcgagggt attttgacaa
     3001 tatcttttat cttcgtttgc tgagaaaggt tccgtccttt tttgctgggc aacatattcc
     3061 actaggagtt gaggatggtg agatgatgat attcttctct tttctcctat ctcatcgcat
     3121 tcttcctctt catactatgg agtatattct tggttttgga gggcagttgg cagatttact
     3181 gacgcaattg attcaagaaa tgaagaagga ggaactattg ggggattata cagaggacca
     3241 tgtcacctat gaactcagtc agctttgtgc tcaagtctat ctctataagg gctatatttt
     3301 acaggatcgc tacaagtacc agttagagaa tcgtcatcca tatttactga tggaacatga
     3361 ttttaaagag acagcagagg agatttttca tgctctacct gcttttcaac aggggacaga
     3421 tttagataag aagattctct gggaatggct ccagttaatc gaatatatgg ctgaaaacgg
     3481 tggccagcat atgcggattg gtctggattt gacatctggt tttcttgtct tttcaaggat
     3541 ggcagccatt ttgaaacggt atttggaata caatcgtttt attaccattg aagcttatga
     3601 ccctagtcgg cattatgatt tgctggttac caataacccg attcataaga aggaacagac
     3661 accagtctat tatttaaaaa acgacttgga tatggaagat ttggcaggga ttcgtcagtt
     3721 attattcact taaaaggctt ggttaatcta ggtctttttt gtgaaattca cacaatctcc
     3781 tcacattttt ttaaaaatta aaaaaagttg ataaacaaga aagcgcttta ttttgtatac
     3841 tagtaagtgt aaagaggaaa cacctcaaga tctttatcag gaggacagta catgtcacaa
     3901 gaaaaataca tcatggccat tgaccaggga actacaagtt ctcgtgccat cattttcaac
     3961 aaaaaagggg aaaaggttag ctcgagtcaa aaagagttta cccagatttt ccctcaggca
     4021 ggttgggttg agcacaatgc caatgaaatt tggaactctg ttcagtcagt tattgcgggt
     4081 gctttcatcg aaagtggtgt caagccaaat caaatcgagg caatcgggat taccaaccaa
     4141 cgtgaaacaa cggttgtctg ggataagaaa acaggacttc ctatctacaa tgctatcgtt
     4201 tggcagtcac gccagacagc acctttggct gagcaactaa aaagccaagg ttatgtggaa
     4261 aaattccatg aaaagactgg tttgattatt gatgcttact tctctgctac caaggttcgt
     4321 tggattttgg atcatgtaga aggtgctcaa gagcgagcag aaaaagggga attgctcttt
     4381 ggtactatcg atacttggtt ggtttggaaa ttgactgacg gtgcggctca cgtgactgac
     4441 tactcaaatg cagctcgtac catgctttat aacattaaag aactcaaatg ggatgatgag
     4501 attttggaaa tccttaacat tccgaaggct atacttccag aagttcgttc taactccgaa
     4561 atctacggca agacagctcc attccatttc tacggtggag aggtgccaat ctcaggtatg
     4621 gctggggacc aacaagcagc cctctttgga cagttggctt ttgagccagg tatggttaag
     4681 aatacttatg gaacaggctc tttcatcatc atgaatactg gggaagagat gcagttgtct
     4741 gaaaacaacc tcttgacaac cattggttac ggaatcaacg gtaaggttta ttatgccttg
     4801 gaaggttcta tcttcatcgc aggaagtgct attcagtggc ttcgtgacgg tcttcgcatg
     4861 gttgaaaatt caccagaatc tgaaaaatac gctcgtgatt ctcacaacaa cgatgaagtt
     4921 tatgtcgttc cagcctttac aggtctaggc gctccatact ggaaccaaaa tgctcgtggt
     4981 tccgtctttg gtttgactcg tggaacaagc aaagaagact ttatcaaggc gactttgcaa
     5041 tctattgctt atcaagtgcg tgatatcatc gacaccatgc aagtggatac tcagaccgcc
     5101 attcaagtac tgaaggtgga tggtggtgca gccatgaaca acttcctcat gcagttccag
     5161 gcggatattt taggcattga cattgcacgt gctaaaaacc tggaaacaac agctctagga
     5221 gcggccttcc tagcaggttt gtcagtaggg tactggaaag acttggacga gttgaaactc
     5281 ttgaacgaga caggagaact ctttgagcca tctatgaacg aatctcgcaa ggaacaactc
     5341 tacaagggct ggaagaaggc tgtgaaagca actcaagtct ttgcggaagt agacgactaa
     5401 tactggcaga ataaagcgat ttatttagaa agtgtgtaaa tatggaattt tcaaagaaaa
     5461 cacgtgaatt gtcaattaaa aaaatgcagg aacgcaccct ggacctcttg attatcggtg
     5521 gaggaatcac aggagctggt gtagccttgc aggcggcagc tagcggtctt gagactggtt
     5581 tgattgaaat gcaagacttt gcagaaggaa catctagtcg ttcaacaaaa ttggttcacg
     5641 gaggacttcg ttaccttaaa caattcgacg tagaagtggt ctcagatacg gtttctgaac
     5701 gtgcagtggt tcaacaaatc gctccacaca ttccaaaacc agacccaatg ctcttaccag
     5761 tttacgatga agatggagca acctttagcc tcttccgtct taaagtagcc atggacttgt
     5821 acgacctctt ggcaggtgtt agcaacacac caactgcgaa caaggttttg agcaaggatc
     5881 aagtcttgga acgccagcca aacttgaaga aggaaggctt ggtaggaggt ggagtgtatc
     5941 ttgacttccg taacaacgat gcgcgtctcg tgattgaaaa catcaaacgt gccaaccaag
     6001 acggtgccct cattgccaac cacgtgaagg cagaaggctt cctctttgac gaaagtggca
     6061 agattacagg tgttgtagct cgtgatctct tgacagacca agtatttgaa atcaaggccc
     6121 gtctggttat taatacaaca ggtccttgga gtgataaagt acgtaatttg tctaataagg
     6181 gaacgcaatt ctcacaaatg cgcccaacta agggagttca cttggtagtg gattcaagca
     6241 aaatcaaggt ttcacagcca gtttactttg acacaggttt gggtgacggt cgtatggtct
     6301 ttgttctccc acgtgaaaac aagacttact ttggtacaac tgatacagac tacacaggtg
     6361 atttggagca tccaaaagta actcaagaag atgtagatta tctacttggc attgtcaaca
     6421 accgctttcc agaatccaac atcaccattg atgatatcga aagcagctgg gcaggtcttc
     6481 gtccattgat tgcagggaac agtgcctctg actataatgg tggaaataac ggtaccatca
     6541 gtgatgaaag ctttgacaac ttgattgcga ctgttgaatc ttatctctcc aaagaaaaaa
     6601 cacgtgaaga tgttgagtct gctgtcagca agcttgaaag tagcacatct gagaaacatt
     6661 tggatccatc tgcagtttct cgtgggtcta gcttggaccg tgatgacaat ggtcttttga
     6721 ctcttgctgg cggtaaaatc acagactacc gtaagatggc tgaaggagct atggagcgcg
     6781 tggttgacat cctcaaagca gaatttgacc gtagctttaa attgatcaat tctaaaactt
     6841 accctgtttc aggtggagaa ttgaacccag caaatgtgga ttcagaaatc gaagcctttg
     6901 cgcaacttgg agtatcacgt ggtttggata gcaaggaagc tcactatctg gcaaatcttt
     6961 acggttcaaa tgcaccgaaa gtctttgcac ttgctcacag cttggaacaa gcgccaggac
     7021 tcagcttggc agatactttg tcccttcact atgcaatgcg caacgagttg gctcttagcc
     7081 cagttgactt ccttcttcgt cgtaccaacc atatgctctt tatgcgtgat agcttggata
     7141 gcatcgttga gccaattttg gatgaaatgg gacgattcta tgactggaca gaagaagaaa
     7201 aagcaactta ccgtgctgat gtcgaagcag ctctcgctaa caacgattta gcagaattaa
     7261 aaaattaaga aaaaataaaa gaggtggagg gcagcattcc ttgtcgcccg tcccttcttt
     7321 ttaatggaga cagaaagatg atgaatgaat tatttggaga atttctaggg actttaatcc
     7381 tgattcttct aggaaatggt gttgttgcag gtgtggttct tcctaaaacc aagagcaata
     7441 gctcaggttg gattgtgatt actatgggtt gggggattgc agttgcggtt gcagtctttg
     7501 tatctggcaa gctcagtcca gctcatttaa acccagctgt gaccatcggt gtggccttaa
     7561 aaggtggttt gccttgggct tccgttttgc cttatatctt agcccagttc gcaggggcca
     7621 tgctgggtca gattttggtt tggttgcaat tcaaacctca ctatgaggca gaagaaaatg
     7681 caggcaatat cctggcaacc ttcagtactg gaccagccat caaggatact gtatcaaact
     7741 tgattagcga aatccttgga acctttgttt tggtgttgac aatctttgct ttgggtcttt
     7801 acgattttca ggcaggtatc ggaacctttg cagtgggaac tttgattgtc ggtatcggtc
     7861 tatcactagg tgggacaaca ggttatgcct tgaacccagc tcgtgacctt ggacctcgta
     7921 tcatgcacag catcttgcca attccaaaca agggagacgg agactggtct tacgcttgga
     7981 ttcctgttgt aggccctgtt atcggagcag ccttggcagt gcttgtattc tcacttttct
     8041 agtttatact cttcgaaaat caaattcaaa ccacgtcagc gtcgccttac cgtactcaag
     8101 tacagcttac ggctagcttc ctagtttgct ctttgatttt cattgagtat tagaaaacaa
     8161 ttatgttgat agagcttggg caagagccca atttcagcaa aaaatgaagt aaatcttctc
     8221 ataataaaac gcatcatatc aagcacgaaa attccacgag gtcaactaca gtcagaaagc
     8281 tgaacaacaa gccaaaacgc ccaaaaaagg cggcaaaaag caagcacctg caagcaacgt
     8341 gccgaaatgg tcaaatcctg attatgtcaa cgaattagac ccaaaaatcg ttgatatgct
     8401 agtagaattt cacaagtcac aaggcacttt ggaaactccc gaggcgcaag cagaaatcgc
     8461 ccaaaaacgt gaagaaatcg agcaaaggag agctgagctt gaggataaaa aacaagagct
     8521 tttgaaccgc ttgaacaaat agagtttcgc aagtattatg cttacaaatt acttgagcaa
     8581 ttaactaaaa tataaaccct gcctttatat ctaggcaggg tttatatttt agaaattcac
     8641 gtaggttgtt acggttttta catacccagt atagtttgag tttctatagt attcagtgat
     8701 aaacttccat tttttttgag caacatggat ataagtactt gttatgtagt atggatatgg
     8761 gctttgtgaa tccaagtaag actgataagc ttgtatacca aaatatgctc caccaattat
     8821 tgcaccccat ggacccccca ataaagtacc tatcctacca atcatataac tgattccagc
     8881 accagtcatg aagttagcga atgtgttagc ttgtttattc ccatgtattg tgttgacgta
     8941 attccaaaca ttaggatcgt atgatctaaa agatatattt aggtcgattt cattcttttg
     9001 ataagccata taaaatgccc cattgatata gacgccgtca gcacgtcgtt caatagtgtc
     9061 tacacttcca tctggattga caacctcaag aacttcatcg cttaaaatat ttacttgcgt
     9121 atctccgaac cgcactgatg agccattctc aaactgagcc tcaccagata caactttaga
     9181 gtttgccgat aagctatcat cagcaaaaac aaacaagcga cggggaaatg ctagacatac
     9241 agaaaacaga cataactagc aaacacatgc atttaaacat cttagacata acggaaactc
     9301 ctttgtattt ttgatttttt tcaactttta ttatacaata aaaccaaata aaaagaaagc
     9361 ggtaacaata tgcttaatgc gaaaattttt tatatatttt tatgtttgat cgttatcgaa
     9421 actacaggct tgttgttgtt gaaaagaggt ctcgaaatgg gttatttaga cacagaagct
     9481 attatcctcg cagttttttc atttgctttt tacaacctat gttcattcgc ttgggtctgc
     9541 tctacaataa aaaacaataa aaaataaata gacgtatttt caaaaaaaaa aaaaaaaaat
     9601 gcatatttat cttagcaaaa cgacaattta aatcgtcgtt tttttgtagt acgacgggca
     9661 tgtcgtatat ctgaggtgta agtcctcggt gggcacctgc taccggtgaa cccaatagcg
     9721 attcccaagc ctgactatcg tgaggtagca gggagaggaa gggatagcga aatcgtggct
     9781 ctacgaacag gaacgtgata gtaaggcgta tatagcggat aaggaggctt caaactctaa
     9841 agtccaaaaa ggtagtcgta acctatatgt gtaaatcacg agagtaattg aattcggact
     9901 aaggtttgtg tgaaaaagat aaatctttct agagtctaaa gactctgcgt cagatttcct
     9961 attttcactg taacctttta acgtcctcat atcttgtata aacgaggaaa gatgtacgac
    10021 ttatcccgtg aggtttcatg agcgctgaaa gcgtagtaac aacgaatcat gagaagtcag
    10081 ccgagcccat agtagtgagg aaacttccgt aatggaagtg gagcgaaggg gtgaatactc
    10141 aaacagtctg gggagagact gtttgaggtc tgtcgctaga aagagaaaac gacagatcga
    10201 agtaatccta cttcacttgt gtctgtaaaa tgagtggtct gatagaactg gactttgaca
    10261 cgta
//
LOCUS       DAVBCB010000066        10186 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVBCB010000066 DAVBCB010000000
VERSION     DAVBCB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.066"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            588..986
                     /locus_tag="VQU28_001847"
     CDS             588..986
                     /locus_tag="VQU28_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2477150.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEVGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKKQTVFLILQQ"
     gene            1024..1713
                     /locus_tag="VQU28_001848"
     CDS             1024..1713
                     /locus_tag="VQU28_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2477151.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQLTLIVQNYWQFSSQIGNFV
                     WIQNILSLLFIGVMIVVLVKTGHGYLFRIPRKKWLWYSILTVLVVVFQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            1755..2003
                     /locus_tag="VQU28_001849"
     CDS             1755..2003
                     /locus_tag="VQU28_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477152.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLKWFVLERLELKDDA"
     gene            2033..2644
                     /locus_tag="VQU28_001850"
     CDS             2033..2644
                     /locus_tag="VQU28_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2477153.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2805..3599
                     /locus_tag="VQU28_001851"
     CDS             2805..3599
                     /locus_tag="VQU28_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HET2477154.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3596..4231
                     /gene="trmB"
                     /locus_tag="VQU28_001852"
     CDS             3596..4231
                     /gene="trmB"
                     /locus_tag="VQU28_001852"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HET2477155.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4357..4836
                     /gene="rimP"
                     /locus_tag="VQU28_001853"
     CDS             4357..4836
                     /gene="rimP"
                     /locus_tag="VQU28_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HET2477156.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4880..6016
                     /gene="nusA"
                     /locus_tag="VQU28_001854"
     CDS             4880..6016
                     /gene="nusA"
                     /locus_tag="VQU28_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HET2477157.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            6038..6331
                     /locus_tag="VQU28_001855"
     CDS             6038..6331
                     /locus_tag="VQU28_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HET2477158.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6324..6623
                     /locus_tag="VQU28_001856"
     CDS             6324..6623
                     /locus_tag="VQU28_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HET2477159.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6640..9432
                     /gene="infB"
                     /locus_tag="VQU28_001857"
     CDS             6640..9432
                     /gene="infB"
                     /locus_tag="VQU28_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HET2477160.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     VKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9683..10033
                     /gene="rbfA"
                     /locus_tag="VQU28_001858"
     CDS             9683..10033
                     /gene="rbfA"
                     /locus_tag="VQU28_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HET2477161.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
BASE COUNT         3093 a         1864 c         2319 g         2910 t
ORIGIN      
        1 attcaagatt tggatgaaaa attttaaaaa acaatgatat actaaacttg tcaaagttgc
       61 aacgggtgct gctacattat gttaaaaatc gctcttgatt gataagctgg atttgttagg
      121 aaaggagtaa ataattatgg ataagaaaaa gatagtttcg actataatat gtatagtatt
      181 tcttgtagtg tcagtagata atttttttag agacttaacc ccgttactgt tcattttaaa
      241 tattattggc ctgtcttgct tttcagtgct aacatacatt aacataaaag agatactctt
      301 aaatataagt aaatgatgat ttggttagtc ctagaaccaa tcaacttatg tttatcggct
      361 tggcagattt tatgtttgtc atttgtttgt atagaggtat ttcagaaaca gaattctatc
      421 aacaactaat cgcttatata ggtgtattct ctgcttgttt atcacgcttc tgctcatgtg
      481 gggcttgaag tattataaaa agtagctttc tatccttttc tgacaggata tttgagtaga
      541 aggctctatt aaattgttag tgaaataaat gaatggaggt atttaatatg aaatacagat
      601 tattttttgt tattttcttg agtagtgtgt tggatattct tttagggaca tttttacaaa
      661 tctctatcgt atctattggg tggcttgttc tttacagtgg tttgtttgaa gtaggagttt
      721 tccttcttgc taataaaggg gtggcggtaa aaatcaagga agtagatatt cgaaatcgct
      781 ttaaatttat ttttggaaaa accttatggt ttcaaattct tttgctcatc tttttgataa
      841 tcaaacttta tcttggtttg gatgcgaggt tgattttatt ctatggacat attttcattg
      901 tctttaatgc cttaatgtat ctattatcta gtagtcaggt tagccttaaa aaaaaacaaa
      961 ctgtcttctt aatcttacag cagtagagtc tgacaaggtg aggtcatagt agggaggatt
     1021 tctatgaaaa agtatcaact tctattcaaa ataagtgcag tcttctctta cttatttttc
     1081 gtatttggtc tttctcagct gacgcttatc gtccaaaatt attggcaatt ttcttcccag
     1141 attggtaatt tcgtctggat tcaaaatatc ttgagtttac tttttattgg agtcatgatt
     1201 gtggttcttg ttaagacagg ccatggttat ctcttccgca ttccaagaaa aaaatggctt
     1261 tggtattcga ttttgacagt attagtggta gtgttccaga tctcttttaa cgttcagaca
     1321 gctaaacatg ttcagtcaac tgcggaaggt tgggctgtat tgattggtta tagtgggact
     1381 aactttgcag agctaggtat ttatatagcc ctgttctttc tggttccact gatggaagaa
     1441 ttgatttata gaggattact gcaacatgct ttctttaagc attcgcgatt tggtcttgat
     1501 ttgcttcttc cttctatttt atttgctctc cctcattttt caagcctgcc tagtctgtta
     1561 gatatcttcg tctttgcaac agttggaatc atctttgctg gtttgacccg ctataccaag
     1621 agcatttatc catcctatgc ggtgcatgtg atcaataata ttgtagcgac cttcccgttt
     1681 ttgctcactt ttctacatag ggtcttgggg taaaaaaaca aaaggcttgc ttttcagcca
     1741 tagaggaggt catcatgtat aaacacttat ttttcctaga ttccaaaact ttagatcggt
     1801 tgacacccta tattctagtc ttggcttctg acaccattgc ttttaatgtt tttgtgctaa
     1861 cctttgtatc tgcggtggtt tttaatttcc taaattccat gctagcttta atggctatat
     1921 tcataggggc tggctatgtg gtcggatttt ggttgctaaa atggtttgtt ttggaaaggt
     1981 tagagctaaa ggatgacgcg taggaagtct gtttggtgag gaggatagtt ttatggagtt
     2041 ttttgataaa tttcatgcct tgtgttttgg atttttagta ctaataattg tcattacagt
     2101 tccttatacg attaaccatg ggggtttttt tcaaaatgaa tctgcattga ttcttgtaag
     2161 tcttcttgta acctcgctga gtgttgctta tgctagaaag tttgaaatga tttcttttgg
     2221 gatgttaagc aagaaacaac ttttgctttt cattgcaatc tttcttctaa gtgtacttga
     2281 gacgctggtt tatattcatt tcttcgctgt ttcttctggc tcaggggtcc aacacttggc
     2341 ggaagtcagc agaggaattt ccctgtcttt gattttgact acctcagttt ttggccccat
     2401 ccaggaggaa ctcattttca gaggacttct tcaaggtgcg gtttttgaca attcttggtt
     2461 agggcttgtg ctaacttcct ctctcttttc tttcatgcat ggaccttcta atgtcccttc
     2521 gtttattttt tatctacttg ggggtttgtt gctgggcttt gcttataaaa agagtcaaaa
     2581 cctatgggtt tctactctag tacacatgtt ttacaacagt tggccactcc tatattattt
     2641 ataaaaatta tgaaaaggta agcagaagac ggtgcttacc tttttcaagc tataatgata
     2701 cccaagccaa tccagaggct tttctttgag aatcgggtta gagtggttga cgaataggcc
     2761 aaaaactagt agaatagtaa ggaaacttta tacggaggaa agaaatggat ttgggtgata
     2821 atgagctaac actgactccc atacctggga aaagtggcaa ggcttatatg ggtagctatc
     2881 ctgacgggaa gcgcatcttt gtaaaaatga acacctctcc aatcctacct ggtctagcta
     2941 gagaacaaat tgctccacaa ttattatgga gtcgtcgttt ggcagatggg cgtgatatgt
     3001 gtgctcaaga atggttgaca ggcaagatat tgacccccta tgatatgaat cgtaagcaaa
     3061 tcgtcaatat tttaacccgt cttcatcgct cacgtccgtt gatgacacaa ttgagtcgtt
     3121 tgggctatgc catggaaaca cctgtagatt tactacagtc ttggcaggaa acggctccag
     3181 atgctttgcg taaaaatcat tttatcagtg aagtgatggc tgatttacgt cagactattc
     3241 caggatttag agaggaccat gcgaccattg tccatggaga tgtacgacat agtaattgga
     3301 ttgagacaga tagtggcttg atttatttag tagattggga ttcggttcgc ttgaccgatc
     3361 gcatgtttga tgtggcccat atgctctgcc attatatttc agaacatcag tggaaggaat
     3421 ggttgaccta ctacggttac aagtacaatc aaacggtatt aagtaaattg tattggtatg
     3481 gtcaattgtc ttatttgagt cagatttcca agtattatat gaaccaagat ttagaaaatg
     3541 tcaatcggga gattcatggt ttgcgtcatt tccgagacaa gtatggaaag agaagatgag
     3601 agttagaaat cgtaaagggg caacagaatt actagaggca aatccccagt atgtggtcct
     3661 caatcccttg gaagccaagg caaaatggcg ggacttgttt ggcaatgata atcccattca
     3721 tgtggaagtt ggaagtggaa agggtgcctt tgtttcaggt atggccaagc aaaaccctga
     3781 catcaactat atcgggattg atattcaaaa gtctgttttg agctacgctt tggacaaggt
     3841 gcttgaagtt ggagtgccta acatcaagct cttgtgggta gatggttctg acttaactga
     3901 ctattttgaa gacggtgaga ttgatcgctt gtatctgaac ttttcagatc catggccgaa
     3961 aaaacgccat gaaaagcgtc gtttgaccta caagaccttc ttggatacct tcaaacgtat
     4021 cttgcctgaa aatggagaaa ttcatttcaa gacggataac cgtggcttgt ttgagtacag
     4081 tttagtgagc ttttctcaat atggcatgaa actcaatggt gtctggttag atttgcatgc
     4141 cagtgatttt gaaggcaatg tcatgacaga atacgagcaa aaattctcaa acaaggggca
     4201 agttatctac cgagttgagg cagaatttta agagatagcc taaaattagg ctgtacaagt
     4261 gcttttgctt tacataagtt ggcaaacgtg ctatactgat agtaagaata tgaaaagtga
     4321 ggcggggaaa tatcttcgcc tcttgcttat gaggaggtgg acgcaatcgc aacaatcgta
     4381 gaattagtca gagaagttgt agaacctgtc atagaagctc cttttgaact cgtggatatc
     4441 gagtatggaa agattggcag tgacatgatt ctcagtattt ttgtagataa acccgaagga
     4501 attaccttga acgacacggc agacttgaca gaaattatca gtcctgtcct agacaccatc
     4561 aagccagatc ccttcccaga acaatatttc ctagaaatta ccagtccagg tttggaacgc
     4621 cctttgaaaa ccaaggatgc cgtcgctgga gcggttggaa aatacatcca tgtcgggctc
     4681 taccaagcca tcgataagca aaaggtcttt gaaggaacct tgttggcctt cgaagaggac
     4741 gagttgacta tggaatatat ggacaagacg cgtaagaaaa ccgtccaaat tccatacagt
     4801 ttagtatcaa aagcacgttt agcagttaaa ttatagaaaa agaaaggata gcttttgagg
     4861 attcaaaagt gaagaaaaca tgagtaaaga aatgctagag gccttccgca ttttggaaga
     4921 agacaaggga atcaaaaaag aagatatcat cgacgcagta gtagagtcgc ttcgttccgc
     4981 ttatcgcaga cgctatggtc agtcagacag cgtagctatt gacttcaacg aaaaaacagg
     5041 tgactttaca gtttatactg tccgtgaagt tgttgatgaa gtatttgata gccgtttgga
     5101 aatcagcttg aaagatgctc ttgccattaa ttcagcttat gaacttggag acaaaatcaa
     5161 gtttgaagaa gcaccagctg agtttggtcg tgtagcagcc cagtctgcca aacaaaccat
     5221 catggaaaaa atgcgcaagc aaacacgtgc catcacttac aatacttaca aagaacatga
     5281 gcaagaaatc atgtctggta cagtagaacg ctttgacaac cgctttatct atgtcaacct
     5341 tggtagcatc gaagcccaat tgtcaaaaca agaccaaatt cctggagaag tttttgcttc
     5401 tcatgatcgt atcgaagttt atgtttacaa ggttgaagac aaccctcgtg gtgtgaacgt
     5461 ctttgttagc cgtagtcatc cagaaatgat caaacgttta atggagcaag aaattccaga
     5521 agtttatgat ggaactgttg aaatcatgag cgtggctcgt gaagcaggtg accgtacgaa
     5581 ggttgctgtt cgtagccaca atccaaacgt ggatgctatc ggtacaatcg ttggacgtgg
     5641 tggtgctaat atcaagaaga ttactagcaa attccaccca gctcgttacg atgctaaaaa
     5701 tgaccgcatg gtaccaatcg aagaaaatat cgatgttatc gagtgggtag cagatccagc
     5761 tgaatttatc tacaatgcca tcgctcctgc tgaggttgac caagttatct ttgatgaaaa
     5821 cgacagcaaa cgtgccttgg tggttgttcc agataacaag ctttctcttg ccattggtcg
     5881 tcgtggacaa aacgtgcgct tggcggctca cttgactggt taccgtatcg atatcaagtc
     5941 tgctagcgaa tttgaagcca tggaagacgc tgcttcagta gagttggaag tagaaaacga
     6001 tactgtagaa gaataaaagc tgctagagga gggaaagatg aaaacaagaa aaatcccttt
     6061 gcgcaagtct gttgtgtcta acgaagtcat tgataagcgt gatttgctcc gcattgtcaa
     6121 gaacaaggaa ggacaagtct ttattgatcc tacgggcaag gccaatggcc gcggcgctta
     6181 tatcaaacta gacaatgcag aagccctaga ggcgaaaaag aagaaggtct ttaaccgcag
     6241 ctttagcatg gaagtggaag aaagctttta tgacgagttg atcgcttatg tggatcacaa
     6301 agtgaaaaga agagagttgg gacttgaata agcaaaagat aagtaatctc ttggggcttg
     6361 ctcagcgagc agggcgcatc atatcgggtg aagaattggt ggtcaaggcc attcaagacg
     6421 gcaaggccaa gttggtcttt ctagctcatg atgctggacc caatctgacc aagaagattc
     6481 aagataaaag tcattattat caagtagaaa ttgtaaccgt gttttcaaca ctggaattaa
     6541 gcatagcagt cgggaaatcg agaaaggttt tggctgtaac agatgctgga tttacaaaga
     6601 aaatgaggtc tcttatggaa tagaagagga ggacatgatt tgtctaagaa aagattgtac
     6661 gaaatcgcaa aagaacttgg aaaagaaagt aaagaagttg tagcgcgtgc aaaagagttg
     6721 ggcttggatg tgaaaagcca ctcatcaagt gtggaagaag ctgtcgctgc aaaaattgct
     6781 gccagcttta agcctgcagc tgctccgaaa gtagaagcaa aacctgcagc accaaaagta
     6841 agtgcagaaa agaaagccga aaaatctgag ccagctaaac cagctgtagc taaggaagag
     6901 gcaaaaccgg ctgagccagt tgctccgaaa acagaaaaag tagcagtgaa accgcaaagt
     6961 cgtaatttca aggctgagcg tgaagcccgt gctaaagagc aggcagaacg acgcaagcaa
     7021 aataagggca ataaccgtga ccaacaacaa aacggaaacc gtcagaaaaa cgacggccgt
     7081 aatggtggaa aacaaggtca aagcaaccgc gacaatcgtc gctttaatga ccaagctaag
     7141 aagcagcaag gtcagcaaaa acgtagaaat gagcgccgtc agcaagagga taaacgttca
     7201 aatcaagcgg ctccacgtat tgactttaaa gcccgtgcag cagccctaaa agcagagcaa
     7261 aatgcagagt acgctcgttc aagtgaggaa cgcttcaagc agtatcaggc tgctaaagaa
     7321 gccttggctc aagctaacaa acgcaaggaa ccagaggaaa tctttgaaga agcggctaag
     7381 ttagctgaac aagcacagca agttcaagca gtggttgaag tcgtccctga gaaaaaagaa
     7441 cctgcagtgg atacacgtcg taaaaaacaa gctcgaccag acaaaaatcg tgacgattat
     7501 gatcatgaag aagatggtcc tagaaaacaa caaaagaatc gaagtagtca aaatcaagtg
     7561 agaaatcaaa agaatagtaa ctggaataac aacaaaaaga acaaaaaagg caataacaag
     7621 aacaaccgta atcagactcc aaaacctgtt acggagcgta aattccatga attgccaaca
     7681 gaatttgaat atacagatgg tatgaccgtt gcggaaatcg caaaacgtat caaacgtgaa
     7741 ccagctgaaa tcgttaagaa acttttcatg atgggtgtca tggccacaca aaaccaatcc
     7801 ttggatgggg aaacaattga actcctcatg gtggattacg gtatcgaagc caaacaaaag
     7861 gttgaagtgg ataatgctga catcgaacgt ttctttgtcg aagatggtta tctcaatgaa
     7921 gatgaattgg ttgagcgtcc accagttgtt actatcatgg gacacgttga ccacggtaaa
     7981 acaacccttt tggatactct tcgtaactca cgtgttgcga caggtgaagc aggtggtatt
     8041 actcagcata tcggtgccta ccaaatcgtg gaaaatggta agaagattac cttccttgat
     8101 acaccaggac acgcggcctt tacatcaatg cgtgcgcgtg gtgcttctgt taccgatatt
     8161 acgatcttgg tcgtagcggc agatgacggg gttatgcctc agactattga agccatcaac
     8221 cactcaaaag cagctaacgt tccaatcatc gtagccatta acaagattga taaaccaggt
     8281 gctaacccag aacgcgttat cggtgaattg gcagagcatg gtgtgatgtc aaccgcttgg
     8341 ggtggagatt ctgaatttgt tgaaatctca gctaaattca accaaaatat cgaagaattg
     8401 ttggaaacag tccttcttgt agctgaaatc caagaactca aggcagaccc aacagtgcgt
     8461 gcaatcggta cggttatcga agcgcgcttg gataaaggaa aaggtgcggt cgcaaccctt
     8521 cttgtacaac aaggtacctt gaatgttcaa gacccaatcg ttgtcggaaa taccttcggt
     8581 cgtgtccgtg ctatgaccaa cgaccttggt cgtcgtgtta aagttgctgg accatcaaca
     8641 ccagtctcta tcacaggttt gaacgaagca ccgatggcgg gtgaccactt tgccgtttac
     8701 gaggatgaaa aatctgcgcg tgcagcaggt gaagagcgtg ccaaacgtgc cctcatgaaa
     8761 caacgtcaag ctacccaacg tgttagcctt gaaaacctct ttgataccct taaagctggg
     8821 gaactcaaat ctgttaatgt tatcatcaag gctgatgtac aaggttctgt tgaagccctt
     8881 tctgcctcac ttcaaaagat tgacgtggaa ggtgtcaaag tgactatcgt ccactcagcg
     8941 gtcggtgcta tcaacgaatc agacgtgacc cttgccgaag cttcaaatgc ctttatcgtt
     9001 ggtttcaacg tacgccctac accacaagct cgtcaacaag cagaagctga cgatgtggaa
     9061 atccgtcttc acagcattat ctacaaggtt atcgaagaga tggaagaagc tatgaaaggg
     9121 atgcttgatc cagaatttga agaaaaagtt attggtgaag cggttatccg tgaaaccttc
     9181 aaggtgtcta aagtgggaac tatcggtgga tttatggtta tcaacggtaa ggttgcccgt
     9241 gactctaaag tccgtgttat ccgtgatggt gtcgttatct atgatggtga actcgcaagc
     9301 ttgaaacact ataaagacga cgttaaggaa gttacaaacg gccgtgaagg tggattgatg
     9361 atcgacggct acaatgatat taagatggat gatgtgattg aggcgtatgt catggaagaa
     9421 atcaagagat aagatttttt gctcctttct taggtggtga gggacgcaag caaaccgatg
     9481 gtttcattgc ttatttttga gcctagggtc tcaaaaatcc cctgtgatgg gactgataaa
     9541 tcagttccat cactttcacc acggcgaaag aagcagatga cttcaaattg aacttcgttt
     9601 caatttaaac tgaaaatcaa gaagtttaaa atagctaggt ctgctggcct agcttttggt
     9661 tcaaagtaga gaaaggaata tcatggcaaa tcatttccgt acagatcgtg tgggcatgga
     9721 aatcaagcgt gaagtcaatg agattttgca aaagaaagtc cgtgatccac gtgtccaagg
     9781 tgtgaccatc acagatgttc agatgctggg tgacttgtct gttgccaagg tttattacac
     9841 cattttgagt aaccttgctt cggataacca aaaagcccaa atcgggcttg aaaaagcaac
     9901 tggtaccatc aaacgtgaac ttggtcgcaa tttgaaattg tacaaaatcc cagatttgac
     9961 cttcgtcaaa gacgagtcca tcgagtatgg aaacaagatt gacgagatgc tacgcaatct
    10021 ggataagaac taaagaagag gggttgcccc tctttttcgg ctctttgtca actgtagtgg
    10081 gttgaagaaa agctaagctc gagaaaggac aaatttcgtc ctttcttttt tgatattcag
    10141 ggcgataaaa atccgttttt tgaagttttc aaagttccga aaacca
//
LOCUS       DAVBCB010000067         9472 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVBCB010000067 DAVBCB010000000
VERSION     DAVBCB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9472
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.067"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            150..686
                     /locus_tag="VQU28_001859"
     CDS             150..686
                     /locus_tag="VQU28_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HET2477162.1"
                     /translation="MKKAHVYAIPAIGAALIAALAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALAVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            737..823
                     /locus_tag="VQU28_001860"
                     /pseudo
     CDS             737..823
                     /locus_tag="VQU28_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(892..1107)
                     /locus_tag="VQU28_001861"
     CDS             complement(892..1107)
                     /locus_tag="VQU28_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HET2477163.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            1205..1525
                     /locus_tag="VQU28_001862"
     CDS             1205..1525
                     /locus_tag="VQU28_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477164.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(1556..1894)
                     /locus_tag="VQU28_001863"
     CDS             complement(1556..1894)
                     /locus_tag="VQU28_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477165.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(1906..2625)
                     /gene="trmD"
                     /locus_tag="VQU28_001864"
     CDS             complement(1906..2625)
                     /gene="trmD"
                     /locus_tag="VQU28_001864"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HET2477166.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(2615..3133)
                     /gene="rimM"
                     /locus_tag="VQU28_001865"
     CDS             complement(2615..3133)
                     /gene="rimM"
                     /locus_tag="VQU28_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HET2477167.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKRYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(3164..3952)
                     /locus_tag="VQU28_001866"
     CDS             complement(3164..3952)
                     /locus_tag="VQU28_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HET2477168.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPEMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFEDVSIEAHIPVFVFCQP
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            complement(4529..4768)
                     /locus_tag="VQU28_001867"
     CDS             complement(4529..4768)
                     /locus_tag="VQU28_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HET2477169.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(4788..5060)
                     /gene="rpsP"
                     /locus_tag="VQU28_001868"
     CDS             complement(4788..5060)
                     /gene="rpsP"
                     /locus_tag="VQU28_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HET2477170.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            5230..5439
                     /locus_tag="VQU28_001869"
     CDS             5230..5439
                     /locus_tag="VQU28_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477171.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            5918..6139
                     /locus_tag="VQU28_001870"
     CDS             5918..6139
                     /locus_tag="VQU28_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477172.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            6222..7025
                     /locus_tag="VQU28_001871"
     CDS             6222..7025
                     /locus_tag="VQU28_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HET2477173.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQSSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(7081..8622)
                     /locus_tag="VQU28_001872"
     CDS             complement(7081..8622)
                     /locus_tag="VQU28_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HET2477174.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMEMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DLPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(8624..9106)
                     /locus_tag="VQU28_001873"
     CDS             complement(8624..9106)
                     /locus_tag="VQU28_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HET2477175.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVYFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(9106..>9472)
                     /locus_tag="VQU28_001874"
     CDS             complement(9106..>9472)
                     /locus_tag="VQU28_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="50S rRNA methyltransferase"
                     /protein_id="HET2477176.1"
                     /translation="KLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALELAELLKNI
                     KKSSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGGNCVVRQEHGTDIDAACGQ
                     LLFNTMKRDRQKAVAAVNP"
BASE COUNT         2796 a         2028 c         1698 g         2950 t
ORIGIN      
        1 attttcgaat caacaaaaaa attatccaac ttgactagtc acgaaaatcg gctgttacag
       61 tttttgttac cttgttttta aaaaatggtt gacatcgact cctatatgag tataatagtt
      121 atcatacatt agaaaagagg ttaactattt tgaaaaaagc tcacgtttat gctatccctg
      181 ctattggggc tgctctcatt gctgctttgg cacaaatcag tcttccaatt ggacctgttc
      241 ccttcactct gcaaaacttt gcaatcggct tgattgctac tgtctttaga ccgagagagg
      301 ctgtactttc tgctggactc tatcttcttc taggtgctat cggtcttcct gtctttgcag
      361 gaggtggagc tggttttcag gctttagttg gccctactgc aggctatctt tggttttatc
      421 tcgtttactc tggacttact tcctctctaa ccaacagcaa gagtggtgtt gttaagattt
      481 ttcttgcaaa cctcttgggt gatgcccttg tctttgtcgg cgggattctc agcttgcatt
      541 tcctagctgg aatggcattt gaaaaagctc ttgctgtggg ggttcttccc tttatcattc
      601 cagaccttgg caaacttctg gctattagtt ttattagccg tcccctactt caacgcctta
      661 aaaatcaggc ttactttact aactaaaaaa ggatatcgag ttatcatgac tcaatatcct
      721 tttcttttat tttgaaaact tatactcaat gaaaatcaaa gagcaaacta gaaagctagc
      781 cgcaggctgc tcaaaacact gttttgaggt tgtggatgaa actgacgaag tcagtaacca
      841 tacatacggc aaggcaaagc tgacgcggtt tgaagagatt ttcgaagagt attagttgcc
      901 tttaaaggca tccaccatcg tttgaaattc ttcatttgag agagtaatcc ctttgcccat
      961 tttagtatgg tctggactcc aagcacgaat atcaaacttt gcaggggcac cattaaagct
     1021 cacacggtta atttccttgg tccaaccttt ttcgttttca gaaagagtca acaagtgctc
     1081 ttcgatttca aatgtaaatt ctgccatttt cttctccttt tttagtttca ttagtttatt
     1141 cgtaaaatct tgtagatttt aggaaaattt tatataatat tgatataaaa gaagggaggc
     1201 caatatgaga cataaattcc agcaagttct aaataaaata catgattttt taaatggata
     1261 tgaccaacct gaccagactg aaaccaactc ccttacagcc actattgaag aggctatcca
     1321 gaaacaaacc gctgttcacc ttatcttgtc tgagacaagt tttacaggtg atatcatcaa
     1381 atatgatcag caaggccagc aaattatcgt gaaaaatttt gccaaaaatg tgagccggat
     1441 tatccgtata agcgatattc aacgcctgcg atttgtcccc tcaactgtcc aaacagccca
     1501 aaaaaataga tttaagaaag agtgagatgt agttgcttca tcccactctt ttttcttagc
     1561 gaatttgttc aaaatgtaaa tgaactgcaa tatgatctcc ataaccactt ctttccaagt
     1621 cacgttgtaa acgataggaa atgtagtgtt ctgcaatggt aatgtaacct gcgcccaata
     1681 aacgatgttc aaccatagat tgaatcatac tgatagtcgc acgttccacc ttggcttctt
     1741 gtaaatccaa aactaccttc ttagtgactt gagcaagatt ttgacgcaaa tcatctgtca
     1801 aaacataaac agtttgggct gccttcaaga tggcttggta aatcttatct ggattaaatt
     1861 cagcaatttc gccattacgt ttgattactt gcataggttt ctcctttatt ctttgttttc
     1921 tttgatttct gccagcattt tttcttcttc tactgtcagt tgataatgtt caagtaaatc
     1981 cggtctgcgc tcgtaggttt tctttaaact ctcgtacaat cgccactgac gaatcttttc
     2041 atggtggcca ctcatcaata catctggcac gaccatgcct cgataatcat agggacgtgt
     2101 gtactgagga tattctaaaa gacctgaaga aaaactatca tcttggtggc tagactcctt
     2161 gccaatcact tctggaatca ggcgaactgt agcatcaatc atggtcatag ctgccaattc
     2221 tccaccagtg aggacatagt cgcctaggga aatctcatct gttaccaagg tcttaatgcg
     2281 ctcatcataa ccctcatagt gcccacagat aaagattagc tcttcctctt gagccaaatc
     2341 ttcagcataa gcctgatcaa actgctttcc agcaggatcg aggagaataa cgcgcggatt
     2401 tttcttttca atagcatcaa aggaatcgaa aataggttgt gctctgagca acatgccctg
     2461 accgcctccg tagggctcat catctacatg acgggccttt tcagcatttt ctcgaaaatt
     2521 atgatactgg atatccaaga gccctttttc tcgagccttt ccaacgattg agtgctccag
     2581 tggagaaaac atctctggaa agagggttaa aatatcaatc ttcatcgtct aacccttcta
     2641 agatttccac atcgacccgt ttatttggaa tatcaacatt gagaaccact ggtgggatat
     2701 aaggtaaaag caaatcacgt ttgccttttc gtttgaccac ccagacatca ttagcacctg
     2761 gttgcaggat ttccttgatg gttccaacca agctatcacc ctcatagact tccaaaccga
     2821 taatctcgtg atagtaaaat tcaccatcgt ctaggtcatt caaatcttcc tcagcgacct
     2881 tgagactata tctcttgtac ttttcgatag tattgatatg gtacatatct ttgaatttaa
     2941 taatgtcaaa gttcttctgt ttacggtggc tagcgatggt cactgtttgg acaaactgat
     3001 ctttttcatc aaacaaagcc agctcagctc cttttttaaa ccgttcttct gcaaaatccg
     3061 tcacagacaa gactcgcatc tcaccctgta atccctgcgt attaacgatt ttcccaacat
     3121 taaagtagtt catcttgtct cctgtaatct ccttttttcc atcttattct gacaattctc
     3181 gaataatagc cgcaattttt tccgattctg accattgtaa ataatggtga ttccctccta
     3241 aaatgagttt agtattggaa gtccaatatt ctgattctct gtactctttt tctctataag
     3301 gctgacaaaa aacaaataca ggaatatgag cttctataga tacatcctca aaatcttcct
     3361 cagtaatctc tccagatatc tgaaattctg gatcttgatt ttccaactct aagccttttt
     3421 cttgcattaa ttcccagatt tttttattcg tttcaggact aaatgttgct tgagttaagt
     3481 tcttaaaata aagttcagga ccacactcgt caatcagcct catctgctct tccatttctg
     3541 gataaggatt ttctgaaaaa tcagcaaaca tgactttttt agttgtcggt tcaattgcta
     3601 ctaaagtctg acgcttaatt ggtttctcga gtaatttgca agctaaaatt ccactccaac
     3661 tatgtgcaca aagtatatat tcagaaattc ctaattcttc aagtacttca taaaccgcat
     3721 ctgcaagatt atctagattt tttccagctt ggtcatgaat cggactccta cctgtgttcg
     3781 gaaaatcaat tgtcaaataa ccaattgtag gaggaggttt ttcaagtata agtgaaaaat
     3841 tttcataact tggtagcaaa cctgctccgt ttagacaaac tagcactttc ttttgctttt
     3901 gataagtaac agagaggcta ccaatttctg tagatacttc aaacctcttc ataaagaaat
     3961 ccactcattc tatataatga attattaaaa atccttatct tttattttat cacgttccaa
     4021 ggattttctc aagttggagg aaggggacaa catctctact ttcccttcaa aaatccttcc
     4081 aaatcatgtt tatgttggta attaacggct gcggttttgt ctttctcaaa gacagtcttg
     4141 gtaaggtcaa tatgattaat agctacgatt gcgacggtgt agtaaatgat atcagccagt
     4201 tctctggcaa gttcctcgtt cgaatcctat cccttctttt cgaccagagc gcctattcaa
     4261 aacctcggct acttctccga cttcctccac taacttcata aagagacctt catcagtccg
     4321 agactgctgt taatgttcga ttaagtagtc ttggaattgc ctaaacgttc aatcttttat
     4381 agtatattga aactagaata gtacaccttt acttctaaaa cattgttaga aatcgatttg
     4441 actgtcctga tcgatttgtc ctgttcttgt ttcattttac tatatcttct attccacaca
     4501 aaaaagcgag acatccgtcc cgcccttctt atttttcgtc aataacgatt cttacttttt
     4561 tgtattcagt tgggacagag tagacaatcg ttcttatcgc agaaatagtg cgacccttac
     4621 gaccgattac acgacccaca tcgctttgat caagattcaa atgatattcc aaaaattctg
     4681 gtgtatcctc aatcttgata gttaaggcat ctggttgtga aattaagggt ttcacaatcg
     4741 caataatgag attttcaatc gtatccatct gtcaacctac tttaaactta ttttgaaaat
     4801 ttagaatcgt ggaatttttt caatacgcct tcttttgaaa ggatgttacg tactgtgtct
     4861 gaaggttgag ctccattagc caaccatgca agaacgcggt cttctttcaa agttacttgg
     4921 ttttcagcaa caagtgggtt gtaagttcca actgtttcga tgaaacgtcc gtcacgtggt
     4981 gaacgtgaat ctgctacgtt gatacggtag aaaggttttt tcttagaacc catacgagtc
     5041 aaacggattt taactgccat ttttaaagtc tcatttcttt aattttttat ttcggtgaaa
     5101 tagctgagct atttagcaca tgttctatta tagcagattt ctggcatgtg tcaagaaaaa
     5161 aacttgacag actataaatt tcttaaagta tttagaaatt tgttagaata ggaaataaat
     5221 gtttgaaaga tgctatccgt gaatactatt ttagaaaaat tttataaaga acaccaagtc
     5281 aaacccttca tatcgcctga acgagagttg gatacttggc ttctaagtcc caagcccgtt
     5341 cccaagcgaa atatggactt attagtagat gattcactag caggagatat cattttacta
     5401 tggagaatcc agtttggtac ttttaccact gaaacatgat tttaaaaact cccattcgaa
     5461 tatgattcaa aattcgctta gaatcaatgt tttcccgctt tttcaaaaaa taaattttca
     5521 tctaatttaa ataactttca attaagtgtg aattttgctt aaaatcatta aaaagcaccc
     5581 catggtgtga accatgaagt gttgtaaatg ctgtgttgta gctagttctt agcgtttaga
     5641 gaactggcta gctttacgag ctttcttaag atctggtttc caaaatactt tgagtacact
     5701 tatggtattg atgcccctaa acatctaaaa actctggtag aaaaagggta tgctcttgtc
     5761 gaaacttctt ttgactcact tgaccatcta aataatacca tgaaaaagaa tatcctgaag
     5821 aagaagggag ttactggact ttctaaaatg aaatcttgcg acctggatca agcccttcat
     5881 gagcattttt cagaagaaga attagctggt cactttcatg tccttctatg gactttttta
     5941 caatggcatt gctatcacac ccaataccta tctaagcgcc tggttcgtaa actttattgc
     6001 agctcttcct ctaaatttcc taattgttga accaattgcc cgttttatac taagttcttt
     6061 tcagaaacca tttactgggg aagaagttga agaagttgaa gattttcaag atgatgatga
     6121 aatcccaact attatctaag ccagttctgt aaactactaa tatttgaaat ccacttcttt
     6181 ttagggtaca atggtataaa tgaatttttg agaggatcag aatgaaaaaa ctagcaaccc
     6241 ttcttttact gtctactgta gccctagctg ggtgtagcag cgtccaacgc agtctgcgtg
     6301 gtgatgatta tgttgattcc agtcttgctg ctgaagaaag ttccaaagta gctgcccaat
     6361 ctgccaagga gttaaacgat gctttaacaa acgaaaacgc caatttccca caactatcta
     6421 aggaagttgc tgaagatgaa gccgaagtga ttctccacac aagccaaggt gatattcgca
     6481 ttaaactctt ccctaaactc gctcctctag cggttgaaaa tttcctcact cacgccaaag
     6541 aaggctacta taacggtatt accttccacc gtgtcatcga tggctttatg gtccaaactg
     6601 gagatccaaa aggggacggt acaggtggtc agtccatctg gcatgacaag gataagacta
     6661 aagacaaagg aactggtttc aagaacgaga ttactcctta tttgtataac atccgtggtg
     6721 ctcttgctat ggctaatact ggtcaaccaa acaccaatgg cagccagttc ttcatcaacc
     6781 aaagctctac agatacctct tctaaactcc ctacaagcaa gtatccacag aaaattatcg
     6841 aagcctacaa agaaggtgga aaccctagtc tagatggcaa acacccagtc tttggtcaag
     6901 tgattgacgg tatggatgtt gtggataaga ttgctaaggc cgaaaaagat gaaaaagaca
     6961 agccaactac tgctatcaca atcgacagca tcgaagtggt gaaagactac gattttaaat
     7021 cttaaaaacc aaaaaaatac agtatccaca ttcggtactg tatttctttt actctcattc
     7081 ttaagttaaa ttattaaaat cccatatttg gtctatccag ccttcataaa agtctggctc
     7141 gtggcagacc ataaggatag atcccctata ttctttgaga gcgcgtttga gctcatcctt
     7201 tgcatccaca tccaaatggt tggtcggctc gtccagcact aaaacgttgt tttcacgatt
     7261 catcaagaga cagaaacgaa ccttggcttg ctctccccct gataatactt gaatctggct
     7321 ttcaatatgt ttggttgtca aaccacaacg ggcaagggct gcacggactt ctgcttgatt
     7381 aagggcagga aaggcattcc agacagcttc aagaggagtt tggcgattac cgccttctac
     7441 ttcctgctca aaataaccaa gttctaaata atctccacgc tccacttccc cagcgattgg
     7501 cgagataatg cccaagagac tcttcaagag agttgttttt ccaataccat tagcaccaat
     7561 aatcgcaacc ttttgattgc gttcgaaggt aagatttaaa ggcttagtaa gaggaaggtc
     7621 gtaaccaatt tgcaagttct tggcttggaa gataaagcgc cctggtgtac gagctggttt
     7681 gaaatcaaag gatggttttg gtttctcact ttggagttcg ataatatcca tcttatccaa
     7741 tttcttttga cgagacatag ccatattacg agttgcaaca cgggctttat tacgagccac
     7801 aaagtccttg aggtctgcaa tctctttctg ctggcgttcg taggctgcct ctagctgaga
     7861 tttcttcata gcataaactt cttggaactg gtagtagtca ccagagtaac gcgtcagctg
     7921 ttgattttcc acatgataga caatattgat aacgtcattg aggaatggaa tatcgtgcga
     7981 aatgagaaca aaggcattct catagttttg gagatagcgc ttgagccaat caatatgctc
     8041 agcatccaag tagttggtcg gctcgtccaa cagcaagata tcaggctttt caaggagaag
     8101 ttttgccaaa agcaccttgg ttctttgccc acctgacaaa gaagttacat ccgtatccat
     8161 gccaaagtcc ataacaccaa gagcacgcgc tacttcgtca atcttagcat ccaaggtata
     8221 gaaatcacga ctctccagac ggtcttgaag ttctcctact tcttccatga gagcatcaac
     8281 atccgcaccg tcttcagcca tttccatata gaggtcattg atacgagctt cagctttgaa
     8341 aagctcatca aaagccgtac ggagaacatc acgcaccgac tgtctttcag caaggacaga
     8401 gtgctgatcc aagtaaccag ccgtcacata tttggaccac tcaacctttc cttcatctgg
     8461 cagcatttta ccagtcacga tactcataaa ggttgatttt ccttcaccat tggcaccgac
     8521 caggccgata tgttctccct tgaggagacg gaaggacaca tcttcaaaaa ttgcacggtc
     8581 accaaaaccg tgactcagat ttttaacttc taaaatactc attttaattc cttatcttgt
     8641 ttttatgtaa tcgtttataa aggagccaag ccagatagcc acccaaagtg ttggtccaca
     8701 aatcatcaat ctcaaagacg cgattgaaat caaagaaaaa gtccaagatt aattgcgtac
     8761 actcgattcc aagactcaca agaaaactaa aaagaaggac cttttttgtt ttccgcaaat
     8821 ttggaaatag ataaaggagt tggaaaatca gaggaaaaaa caagaagaca ttgaggatat
     8881 tttgtaaaaa aatccaacat aattgtccaa tgtcactcac ttcgcccagt ttccagagag
     8941 aattgaaagg agtcaaaaga aaaaccaggc gtccaagatg ctgaatatct ggagttccca
     9001 ctcccacggt agattgttct tgaggagtaa agcaaaaata gacaatgcaa atgctataga
     9061 aaatgactcc ccagaccaaa atatgattat aagtcttctt catcattaag gatttaccgc
     9121 tgcgactgcc ttctggcggt cacgtttcat tgtgttaaag agcaattgtc cacaagctgc
     9181 gtcaatatct gtaccatgct cttgacgaac cacacagttg cccccttttt tcttaagcgt
     9241 atcatagaaa gccaacacgc gctctttggg actacggcta tattggtcat gctcactaac
     9301 tgggttataa ggaatcaagt ttacataaga cgatttcttg atgttcttga gcaattcagc
     9361 caattccaag gcttgttcca caccgtcgtt gacttcatta agcatgatat attcaaaggt
     9421 tacacgacgg tttgttgtct caatgtagta ttcaatagca gcaaagagtt tt
//
LOCUS       DAVBCB010000068         9292 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVBCB010000068 DAVBCB010000000
VERSION     DAVBCB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.068"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(135..1103)
                     /locus_tag="VQU28_001875"
     CDS             complement(135..1103)
                     /locus_tag="VQU28_001875"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HET2477177.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(1248..2063)
                     /locus_tag="VQU28_001876"
     CDS             complement(1248..2063)
                     /locus_tag="VQU28_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HET2477178.1"
                     /translation="MKKSSILFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFTNTYIHDLLGVLFPD
                     SPFLNAFESAIVAPLVEEPLKLLPLVFVLALIPVRKLKSLFLLGIASGLGFQMIEDIG
                     YIRTDLPEGFDFTISRILERIISGIASHWTFSGLAVVGVYLLYRAHKEQKVGKKQGLI
                     FLGLALRTHFLFNSPFVELETELPLAIPVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(2088..2585)
                     /locus_tag="VQU28_001877"
     CDS             complement(2088..2585)
                     /locus_tag="VQU28_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HET2477179.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQU28_001878"
     CDS             complement(2658..4019)
                     /gene="radA"
                     /locus_tag="VQU28_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HET2477180.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MSVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(4033..4548)
                     /locus_tag="VQU28_001879"
     CDS             complement(4033..4548)
                     /locus_tag="VQU28_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2477181.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGFPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(4550..4993)
                     /locus_tag="VQU28_001880"
     CDS             complement(4550..4993)
                     /locus_tag="VQU28_001880"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HET2477182.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(5050..5147)
                     /gene="ffs"
                     /locus_tag="VQU28_001881"
     ncRNA           complement(5050..5147)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VQU28_001881"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5180..5647)
                     /locus_tag="VQU28_001882"
     CDS             complement(5180..5647)
                     /locus_tag="VQU28_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HET2477183.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(5848..7134)
                     /locus_tag="VQU28_001883"
     CDS             complement(5848..7134)
                     /locus_tag="VQU28_001883"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HET2477184.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(7365..7601)
                     /locus_tag="VQU28_001884"
     CDS             complement(7365..7601)
                     /locus_tag="VQU28_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HET2477185.1"
                     /translation="MLQKIYEQMTNFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            7867..8664
                     /locus_tag="VQU28_001885"
                     /pseudo
     CDS             7867..8664
                     /locus_tag="VQU28_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8699..9292)
                     /locus_tag="VQU28_001886"
                     /pseudo
     CDS             complement(8699..9292)
                     /locus_tag="VQU28_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         2967 a         2194 c         1504 g         2627 t
ORIGIN      
        1 tgatgacgta cagtcatatt tcttctcttt ttattatatc acagttttaa atctagcttt
       61 actagattca ccgctactat ctatttattc ggaaaaaaga cgaaaaaacc tgagaatcat
      121 ctcaggcttg gtcattaaat ttttttctca atatcgaaaa gtggagaaag tggtcgtttt
      181 tcatgaatac gtacgatagc atcccctagg agatgagcga ttgaaatctg ctcaatctta
      241 tcaatcaaac gctcttctgg cagatagatg gtatccaaaa caaccaattt cttaatagct
      301 gatttttgga tattgtccgt agcaggacca gaaagaactg ggtgcgtaca gcttgcatag
      361 acttcaacag caccagcttc cgcaagagca tctgccgcat gacaaatcgt tccagcggta
      421 tcaatcatat catcaatcaa gatacaagtc ttgccttcaa ccttaccgat gatgttcata
      481 acttcactag tattcatctt atcaacgcta cgacgtttat caataatagc gatagatgtt
      541 ttcaaaaatt ctgccaactt acgagcacga gtcacccctc catggtccgg gctgacaacc
      601 acatagtcag aaccaaccat accacgacgc tcaaaataat ctgcaatcag aggagcaccc
      661 atcaaatgat ccacaggaat atcaaagaat ccttgaattt gcgcagcatg caagtcgatg
      721 gtcaataaac gatccactcc agctacttca agcatatttg cgacaagttt tgaagtgatt
      781 ggctcacgcg ctctcgcctt tctatcctga cgtgcatacc catagtaagg catgacaaca
      841 ttgacagatt ctgcactcgc acgcttcaaa gcatctacca taatcaaaat ttcaagcaga
      901 ttgtcattta caggcgaact agttgattgt aggataaaga cgtgtttccc acggattgat
      961 tcttcaatgt tgacctgaat ctctccatct gaaaattggc gaacacttga tttccccaac
     1021 tctatcccaa tctcctgcgc cacacgttct gccaattctt tattagaaga aagggcaaac
     1081 agctttaaat cagaaaaaga catgatttcc tccggtatat atgtatcgct tgtgcttttc
     1141 acaagatttt ccatctacca ttgtagcgct ttttgcacta tttttcaatc aaaaataaaa
     1201 gaagggcacc atatttgtac ccttgcatca ttcttttgaa aaatattcta ggtcatcaac
     1261 tcattgtgtt tctcaacaaa gcaataagca tgataaaaac catagagagc aatagccgta
     1321 accactggaa tcgctaaagg caactctgtt tccaactcca caaaaggaga gttaaacaag
     1381 aagtgagttc tcaaggctaa acctagaaaa ataaggccct gtttcttgcc aaccttctgt
     1441 tctttatggg ctctgtaaag caagtaaaca cctactacag ctagacctga aaaagtccag
     1501 tgagaggcaa ttcctgagat gatacgctct aaaattcgcg aaatagtaaa gtcaaagccc
     1561 tctggcaaat ccgtacgaat ataaccaata tcctcaatca tttggaatcc caaaccggaa
     1621 gcaattccaa gtaaaaacaa agattttaat tttcgcacag gaatcaaagc caaaacaaaa
     1681 acaagtggca ataatttcaa gggttcttct accaaaggag ccacaatagc actttcaaag
     1741 gcatttaaaa atggactatc tgggaaaaga acccccagta aatcatggat ataagtatta
     1801 gtaaaactag acaaccagcc tgaaaggaac atccctccca ataaagacag aatcaaaacc
     1861 ttctttggca attcccattt tttcccaata cggaagagaa aataaagagc cggaatcatg
     1921 taaaagagag ctagaaagat agaaactccc attagtccat attccgcacc tgacctcgaa
     1981 ccgtccgtat agtagatggt ttcatactgt aaaccaatac atagcaataa aataaaaata
     2041 aataaaatac tgcttttctt catacacttt ctttctaaat gaagtattta taattctacg
     2101 actgtcatac ttcctgtatc aacattgtaa atggcaccag agataatgac atcgtctggt
     2161 attaggggag actcgataag cagttgcata tcctcgcgta cactctcttc tatatcttgg
     2221 aagggcaaga agtcctggtc tgacacatct acacctaatt cctcttttaa atactcctga
     2281 aaaggttcat tttcaaaggt ctgagcacca cagtctgtat ggtgcaatac cacaatctct
     2341 cttgtcccca tttgttgctg ggaaataact agcgaacgaa tcatgtcttc agtcactcga
     2401 ccacctgcat tccgcaagat atgagcatcc ccaagtgcca agcccagagc ttgcgcaacg
     2461 tgcagacgag agtccataca ggtcacaata gctactcttg ttttgggttt aagtggcaga
     2521 tttaactgcc catgtagggc aacataagcc tgattagctt gcataaactg ttcaaaatac
     2581 gacacgattc cctccttgaa aatttgatag tcaaatattt ctcctatctt atcattttta
     2641 agagaatttg tcacggatta tgcaaagacc tttttcaaaa cttcctgaat cgttgtcacg
     2701 ccaatgacct gaatttcctt aggcagagtg attcctgtca aggaattctt aggtacataa
     2761 atcttagtaa agcccagttt agcagcttcg ttgatgcgtt gctcaatacg attcacgcgc
     2821 cgaatctctc ctgtcaagcc cagttctccg acaaaacatt cctgaggatt agttggcttg
     2881 tctttgtagc tcgaagcaat agcaactgca acagccaagt caatggcagg ttcatccaat
     2941 ttaacaccac cagcagattt gagataggca tcctgatttt gcaagagaag ccctgcccgt
     3001 ttttccaaaa cagacataat caagctagca cggttaaaat caagtcctgt cgtagtacgc
     3061 ttggcatttc caaacatggt cggtgttacc aaagcctgaa cctccgccaa aatcggacgc
     3121 gtcccttcca tggttacaac gatggaggaa ccagtcgccc catccaaacg ctcttctagg
     3181 aaaacttgac tcggattgag tacctcaacc aagccgcccg actgcatctc aaaaatccca
     3241 atctcattag tggaaccaaa acgatttttg accgctctca aaatacgaaa ggtgtggtga
     3301 cgctcccctt caaagtaaag caccgtatcc accatatgct ccaacatacg aggcccagcc
     3361 aaggttcctt ctttggtcac atgacctacg ataaagatgg caatgttatt ggtcttggcc
     3421 aactgcatga gttcagcggt cacttcacgc acctgagaaa cagacccctg cacccctgaa
     3481 atctcaggag acatgatggt ctggatggaa tcaataatga gaaagtctgg ctggatacgc
     3541 tccacttctg cacgaacact ctgcatattg gtctctgcat agagataaaa ctcactatca
     3601 atatcaccta agcgctctgc acgtagttta atctgctggg cagactcctc cccactgaca
     3661 tagagaactg tccccacttg ggacaactgg gttgagactt gtaggagaag agttgatttc
     3721 ccaatcccag gatccccacc gatgaggacg agacttcctg gtaccactcc gcctccaagc
     3781 acacggttga attcctccat ctccgtcttg gttcgattga cattgatgga agtcacctca
     3841 gctagtttca tgggcttggt tttctcacct gtcaaggaca cacgcgcatt cttgacctcg
     3901 gcaacctcaa cctcttccac aaaagaagac caagacccac agttggggca acgtcccaga
     3961 tatttagggg aattataccc acaattttga catacaaatg tcgctttttt ctttgcgatg
     4021 acaaacctct ttctatatct ctaactcaca ctcaatcact tggcaaaaat caatcttctc
     4081 atttggcaca aactggcgca tgagcattcg atgagcaaca actaccacag tctgatgttc
     4141 tcgatactta gacatacatt ctagaaaccg agacttcatt tccgtagctg tctcatattg
     4201 aataggacta ttaggaagca actccccctt gttttctaaa aacagtcttc tagctgtttc
     4261 aaagttttct attcctgttt tatagacctg ccattcatgt aataaaggct ctactcttaa
     4321 aggaaaaccc gtagcacata ccacatacga agccgtttct aaagctcttg tgactgcaga
     4381 agatacgatt atttcagctg acgagagtaa aggatttttg ctcaatttct ggacttgctg
     4441 ccgtcccatc tcagacaagg gtgccaaatc tatcccaaat cctatataag aacgctcctc
     4501 taactcacgg taatctggct ccccatgacg tacaaagata atcttcattc tagtgccctg
     4561 tcgatccaaa tccaccagtt cgaacgccat cagccgcatc tccatctgca attaagaaag
     4621 tagcaaaaac agcctggaca atacgctccc caacttcaag aacaacctct tggtctgtga
     4681 tattcttcat ctgcgcaaaa atatgccctt catttccagg atttccataa taatccccat
     4741 caatgactcc aactgagtta attaaaacca agcccttctt acgaggattt gaagaacgat
     4801 catagaggta gagaacctca gtcggctgca tataagcctt aacccctgtc ggaaccaaga
     4861 caatctctcc tggcgcaaca actgtacgca cagcaacctt taagtcgtaa ccagccgcat
     4921 gcgctgtctc acgcttgggc aataaatttt catctgtaaa actcgaaacc aattcaaaac
     4981 cacgaatttt cataattttc tcttttctat tatcatttat tctagattat tctatcttat
     5041 ttattcggaa aaagcacgaa aaaaagagca cacaattcaa atcgcttagg gctgctggat
     5101 tcctcccctg acccgcttca cgcagaactg ttgctccact atttattata tcacattcct
     5161 attcatttta aaagcaaaat tattttttcc gtctatttct aaaaaaatcc tgcatgatag
     5221 ctgcgcattc atcttccaaa attcctattt caacctccac acgatgatta agacgctcat
     5281 ctgtcaagat atcgtacaaa cttccagcag cgccaaattt ctggttttta gccccataga
     5341 ccacatttgg aatgcgggca agcccaatcg ccccactaca catgacacaa ggttcaatgg
     5401 tcacaaaaag tgtgcaatcc agcaagcgcc agctctcctc actcaagttc gcatcctcta
     5461 tagccataat ttccgcatgc ataaccgctc gctgtaattc ctcacgcgca ttatgcccac
     5521 gaccaatgat ttccccatct ttgacaatca cacaaccaat tggaatttca tcgtgttcaa
     5581 gagcaatctc agcctctctc aaagcctccc tcataaagac ttctttttct tcaagcgtat
     5641 aatacatctc ttttctcttt tcctacttat cgattttatt attatatcat gaatcccaac
     5701 acaaaaaaag ccaccgaatg cggtgactct atagggagat tattatgaaa aagaaaagtt
     5761 taggatattt gttacaacaa gttaggaggt cttcttgtaa ctgtctatag tatacccgac
     5821 ctatcttaaa caaatcttaa aaatctctta ggaccaaaca ctttctaaaa tatttgtttg
     5881 ttcacgacca ggacctactg agaaagtaga aatacgaacg ccaaccaatt cactcacacg
     5941 acgaacatag ttacgcgcat tctcaggaag atcttccaaa ttgcgagctc cggtaatatc
     6001 ttctgaccaa cctggcaact cttcatagat aggcttgcaa cgtttcaatt gctcaagact
     6061 agctggatag tagtcaatac gttgaccgtc aagatcatag gccacacaaa ttttcacagt
     6121 atccaagccg ctcaaaacat caatagagtt caaagaaagg ttagtaatac cagaaacacg
     6181 acggctatga cgcatcacaa ctgagtcaaa ccaacctaca cgacgtggac gaccagttgt
     6241 tgtaccatat tcatgaccca cttcacggat acgttctccc acttcatcaa acaactcagt
     6301 tgggaaagga ccatctccta cacgactcgt ataagcttta catacaccta caaccttgtc
     6361 aatcttgctt ggaccgatac cagaaccaat tgtcacacca ccagctacag ggtttgatga
     6421 cgtaacaaat ggataagtac cttgatcgat atctagcata acaccttgtg caccttcaaa
     6481 aagcacacgt ttgccattat caagcgcatc attcaagata acagatgtat ctatcacgta
     6541 tttcttgatt tgttgaccat attcgtaata ttcttcaaaa atatcatcga aaacaatcgc
     6601 tttactgtca tacaattttt caaaaagacg attcttttca gcaaggttac gttctaaacg
     6661 ctcacggaaa atatctttat ctaaaagatc tgcaatacga attccaacac gagcagcctt
     6721 gtccatataa gctggaccaa ttcccttaat tgtcgtacca atcttattgt cgcccttagc
     6781 ttcttcttgc aagcgatcca actcgatatg ataaggcaaa ataacatgcg cacgatcaga
     6841 aatacgcaag ttatcagttg ttacaccttc ctcatgaaga tagctcaact cttttacaag
     6901 agatttagga tttacaacca taccattccc aatgacagat attttttcag ggaagaaaat
     6961 cccagatgga atcaagtgca acttaaattt cttaccgtca atcacaatcg tgtgaccagc
     7021 attatcacca ccttggtaac gtgcaattac ttctgcattc gctgaaagga agtctgtaat
     7081 cttcccttta ccttcatcac cccattgggt acctacaaca acaactgaag tcataatttt
     7141 gtctgagccc tcaggctctt cctttctcac atacatggca ggactctcac ctgcaattat
     7201 atcttacaat ttattataat aaaaaatcgc ctttttatca agaagaaaca atagaaagat
     7261 ttgctatttc caactattaa aaaatgattt agaaaaatta ctagctattt actattatct
     7321 ttccataaaa gagtaaatta gttcggaaat ttactaaaat tacctcaaca agaaataaaa
     7381 ccccgattca ttaccaattt ttcaagatac aaacgataag caacacgata atggtaaacg
     7441 ataaaatccc tacgacaacc aatgccatat ctcactaaat aataaattaa aaatttaaaa
     7501 tgaacatgtt cccagtcaaa attatcacca aatgtaggac catactcttc ttcaatacta
     7561 tcatagaaat tagtcatctg ctcataaatt ttttgtaaca taatcaaata ctccttttct
     7621 tttttataaa cttattctaa caaaaaattt tacacattca ctatcaattc ctgaattgtt
     7681 aaaataccag cctcaacaag ataaaaatag gaaaagttga caaaatagaa acaaatttgc
     7741 tacctaaatt tcaaacgatt aaagttctaa aaagaccgcc ttaaaacttt tgggtaaatc
     7801 ctattagaac tataatattt atatatatgt attatggttc tgattttcta gtataaaaaa
     7861 attcaaacta gaattcttct tagaaaaggc cgttttaagt gctatcattg ctcaaaaatg
     7921 gaggtcgctg agacttctat cgtcaagaag aatcatcaaa ttccttgtat catcaaccaa
     7981 aagattgctc agaagttaat tgaaaagact tctatgaccg acattgatca tcagttgtct
     8041 atttcaactt caactgtcat tcgcaagatc aataattttc actttgagca tgatttttcg
     8101 cgtcttcctg agattatgtc ctaggacgtt gaaacagtcc ggggagtgac tgtttcaatc
     8161 gggagatgga gatgagcttt attgctcagg actttgataa actcaatatc ataactgttc
     8221 ttgagagcag aacacaagcc atcatccgaa atcccatgaa tacaaggcta tcaagcgcta
     8281 ctggaagctc attcaacaag atagtcagaa actgagtgat aaacgatttt atcgccctac
     8341 ttttcgtatg cacttgacta ataaagaaat tctagataaa cttttgagct attccgaaga
     8401 cttgaaacac cactataatc tctatcagct cttgcttttc cacctctgtt tcagactttg
     8461 tttaaaacct tcctcaaaga taaagagaaa atcgtcaacg cccttcaatt accttattcc
     8521 aacgccaaac tggaagccac caataatctc atcaaactta tcaagcacaa tgcctttggt
     8581 tttaggaact ttgaaaactt caaaaaagaa aggacgaaat ttgtcctttc tagatcttcg
     8641 ctttcttcaa cccactacag ttgacaaaga accgaaaaat gtctcgcctc taatatagtc
     8701 aattgaaaca agaacaagag aaaagagcct cgtaaaaggt attgcaactt ggtaatacct
     8761 ttttgaggtt ctttttgata taagcccatg ttttctcaat aggattgtac tcaggtgagt
     8821 agggaggaag aggtaaaagt ttatgcccaa actcctcgca taaaagttct aacttaccca
     8881 ttctatggaa tcttgcatta tccataataa taaccgatgg tgtggttaat gttggtaaga
     8941 gaaacttctg aaaccaagct tcaaaaaagt cgctcgtcac catctcttcg taagtcattg
     9001 gagcgattaa ctcaccattt gttagacctg caaccaaaga aatcctctga tatcttcttc
     9061 cagatacttt acctcttatt aactgacctt ttaatgagcg accatattct cgataaaaat
     9121 aagtatcgaa tcctgtttca tcaatctaaa caggtgctag gtgctttaaa ctattaaaat
     9181 ttttaagaaa taaggctact ttttctgggt cttgttcata gtaggtgtgg ttcttttttt
     9241 cgagtgtagc ccatagcttt gagcgcatag tggatggtag ttggatgaca gc
//
LOCUS       DAVBCB010000069         9136 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVBCB010000069 DAVBCB010000000
VERSION     DAVBCB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9136
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.069"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..294
                     /locus_tag="VQU28_001887"
                     /pseudo
     CDS             1..294
                     /locus_tag="VQU28_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466308.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            370..819
                     /locus_tag="VQU28_001888"
     CDS             370..819
                     /locus_tag="VQU28_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000337416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HET2477186.1"
                     /translation="MCTILVSIYYKIGLSSSGVYKAVADGEMTVSLSYEDPAVKLLND
                     GANIKVVYPKEGTVFLPASAAIVKKSKNMENAKKFIDFIISQEVQDTLGTTTTNRPVR
                     KNAKTSENMKPIDKIKTLTEDYDYVIKNKSDIVKKYNEVFTDIQSKQ"
     gene            832..1491
                     /locus_tag="VQU28_001889"
     CDS             832..1491
                     /locus_tag="VQU28_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477187.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIEGGEFYFDDTKINNMEPSKRNIGMVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYADRKPDKLSGGQQQRVTLACALAVNPSVLLMDEP
                     LSNLEAKLRLDMRQAIREIQHEVGITTVYVTHDQEEAMAISDQIAVMKDGVIQQIGRP
                     K"
     gene            1618..1923
                     /locus_tag="VQU28_001890"
                     /pseudo
     CDS             1618..1923
                     /locus_tag="VQU28_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809465.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
     gene            1913..3558
                     /locus_tag="VQU28_001891"
                     /pseudo
     CDS             1913..3558
                     /locus_tag="VQU28_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(3661..4353)
                     /locus_tag="VQU28_001892"
     CDS             complement(3661..4353)
                     /locus_tag="VQU28_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HET2477188.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGCEKMEEGSI"
     gene            complement(4597..5934)
                     /locus_tag="VQU28_001893"
     CDS             complement(4597..5934)
                     /locus_tag="VQU28_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HET2477189.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALTETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGAAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     IGDSVARVLEEMGLETVGTHGTTVALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGREGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            complement(5944..6210)
                     /locus_tag="VQU28_001894"
     CDS             complement(5944..6210)
                     /locus_tag="VQU28_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HET2477190.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            complement(6475..6861)
                     /gene="rplQ"
                     /locus_tag="VQU28_001895"
     CDS             complement(6475..6861)
                     /gene="rplQ"
                     /locus_tag="VQU28_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HET2477191.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            complement(6873..7808)
                     /locus_tag="VQU28_001896"
     CDS             complement(6873..7808)
                     /locus_tag="VQU28_001896"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HET2477192.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            complement(7851..8234)
                     /gene="rpsK"
                     /locus_tag="VQU28_001897"
     CDS             complement(7851..8234)
                     /gene="rpsK"
                     /locus_tag="VQU28_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HET2477193.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            complement(8252..8617)
                     /gene="rpsM"
                     /locus_tag="VQU28_001898"
     CDS             complement(8252..8617)
                     /gene="rpsM"
                     /locus_tag="VQU28_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HET2477194.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            complement(8635..8751)
                     /gene="rpmJ"
                     /locus_tag="VQU28_001899"
     CDS             complement(8635..8751)
                     /gene="rpmJ"
                     /locus_tag="VQU28_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HET2477195.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            complement(8776..8994)
                     /gene="infA"
                     /locus_tag="VQU28_001900"
     CDS             complement(8776..8994)
                     /gene="infA"
                     /locus_tag="VQU28_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HET2477196.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
BASE COUNT         2766 a         2044 c         1567 g         2759 t
ORIGIN      
        1 tcaaaagaaa atgataatgt tatcaaagaa tatcaaaaca caactggctt ctctactcct
       61 tatacactag atggtagtgt tttaatcgtc caccctgatt taactaaagg catgaacatc
      121 gaaggatata gcgaccttct caaacctgaa ctaaaaggaa aaatcgcaac tgctgaccca
      181 gcaaactctt ctagcgcctt tgctcaatta acaaatatgc tacaagctca aggtggttac
      241 aaagatgata aggcttggtc ttatgtaaaa gatcttttca cacttattga tggtatagta
      301 aaatgaaata agaacaggac aaattaatca ggacagtcaa atcgatttct aacaatgttt
      361 tagaagtaga tgtgtactat tctagtttca atatactata aaatcggttt aagttcatct
      421 ggtgtctata aagcagtcgc tgatggagaa atgactgtta gtctctctta tgaagatcca
      481 gcagttaaac tcttaaatga cggagctaac attaaggtag tctatccaaa agaaggaacc
      541 gtcttcctac ctgctagtgc tgctatcgtt aaaaaatcta aaaatatgga aaatgccaag
      601 aaatttatcg attttattat ctctcaagaa gtacaagata cacttggtac aaccactact
      661 aaccgtcctg ttcgtaaaaa tgctaaaaca agcgaaaaca tgaaaccaat tgacaaaatc
      721 aaaacactca ctgaagatta tgattatgtc atcaagaata aatcagatat cgttaagaaa
      781 tacaacgaag tctttacaga tatccaatct aaacagtaaa agaggttcac tatgagtgag
      841 atcgaaatta ttaacgccaa aaaaatctac cacgatgtcc ctgttattga gaatttgaac
      901 attacaattc caaaaggaag tctctttacc cttcttggag cttcaggatg tgggaaaacg
      961 acccttcttc gtatgattgc aggtttcaac agtatcgaag gtggagaatt ttacttcgat
     1021 gatacaaaaa tcaataatat ggaacccagc aaacgcaata tcgggatggt tttccaaaac
     1081 tacgctattt tcccacattt gactgtccga gacaacgttg cttttggtct tatgcaaaag
     1141 aaggttccaa aagaagaatt gattcaacag accaacaagt atcttgaact catgcaaatt
     1201 gctcaatatg cggatcgaaa gcccgataaa ctcagtggtg gacaacaaca acgtgtcacc
     1261 ttggcatgcg ccttagcggt taatccaagt gttctcctca tggacgagcc acttagtaat
     1321 ctggaggcca aacttcgctt ggatatgcgt caagccatcc gagaaatcca acacgaagtg
     1381 ggaattacaa ctgtttatgt aacccacgac caagaagaag ccatggctat ttcagaccaa
     1441 attgctgtta tgaaagatgg ggtgatccaa caaatcggcc gaccaaaata actctatcat
     1501 aaaccagcta atgagtttgt ggcaaccttt atcggacgca caaatattat ccctgccaat
     1561 cttgaaaaac ggagcgacgg cgcttatatc gtcttttcag atggctatgc ccttcgaatg
     1621 ccagctcttg atcaggttga ggagcaagct attcatgtaa gcattcgtcc cgaagagttt
     1681 atcaaagatg aatctggaga tatcgaagga actattagcg atagcgtcta tcttggacta
     1741 aatacggagt atttcattga gacaggtttt gcctcaaaaa tttaagttag tgaagaatca
     1801 acttttgaag aagaactaca aaaaggcaat cgtattcgtc tacgaatcaa tacgcaaaaa
     1861 ttaaacatct tttctgcaga tggttcccaa aacctgataa aaggagtcaa ccatggaatg
     1921 taaaaaacta aatatttgga cagcttcctc tttcttcatc tttcttacct atcttgtctt
     1981 tctcgtttat cctatcgtta ccgtgctcaa gcaagcactt atacatgaag gacaattctc
     2041 actagctaat tttgtcactt tctttagtaa agcctactac tctgagacac tagtcaacag
     2101 tttcaaggtt tccattaccg ctactatcac ttccttagtt gtaggaaccc tattagctta
     2161 tctcttctct atgtatgact tcaaggggaa gaaatttcta caaatattga ttatcattgc
     2221 ttccatgtca gctcctttcg taggagccta ctcctgaatt ctcttgctgg gacgaaatga
     2281 ggtcatcact aaatttttga caaatgccct tcatcttcca gctatcgata tttatggatt
     2341 caaaggaatt gtacttgtct ttacactgca actattccca ctggtatttc tatatgttgc
     2401 tggaacaatg aacagtattg acaattctct acttgaagca gctgaaagca tggggtcctt
     2461 cggatttaag cgtatcgtaa cggttgtttt acctctccta gttccaacct tactagcagc
     2521 tccttgcttg tatttatgag agcattctca gactttggaa cgcctatgtt gattgacgaa
     2581 ggatatcgga ctttccctgt cctgatttat acccaattta ttagcgaggt tggaggaaat
     2641 tctgcttttg caattatggc gattatcatt gccttggcaa ttttccttat ccaaaaacac
     2701 attgcaaacc gctacagttt cagcatgaat ctgctccatc caattgagcc taaaaaaact
     2761 acaaaaggaa aaatagctgc catttatgca acagtctacg gaattatctt tatctctgtt
     2821 ttacctcaaa tctacttaat ttatacctct ttcctaaaaa catcaggtat ggtatctgtt
     2881 aaaggttatt ctccaaacag ttacaaggta gctttcaatc gtatgggatc tgctattttc
     2941 aataccattc gtatcccttt gattgcctta gttctagttg ttctatttgc gacatttatc
     3001 tcctacctag ccgttagaaa acggaatttg tttacaaact taattgacag cctcagtatg
     3061 gtaccttata ttgtaccagg aaccgttcta gggattgcct tcatttcttc cttcaatact
     3121 ggtctatttg gaagtggatt tcttatgatt acagggactg ctttcatctt gattatgtct
     3181 ctatctgtca gaagattacc ttatactatt cgctcatctg ttgctagctt acaacaaata
     3241 gcaccaagta ttgaagaagc tgctgaaagc ttaggaagta gtcgtctcaa tacctttgct
     3301 aagattacaa ctccaatgat gctatctggt atcatttctg gagccatctt atcttgggtc
     3361 acaatgattt caaaactctc tacttctatc ctcctctaca atgtcaaaac aagaacaatg
     3421 actgtagcta tttatacaga ggttctcaga ggaaattacg gtgtagccgc agccttgtca
     3481 actatcctga ctgttctaac agtaggttcc ttgctcttgt ttatgaaaat ctctaaaagc
     3541 aatagcatta cactttagtt ttccacatca aaaacagccg aaaggagtgc cctcggctgt
     3601 tttttcttat cttgatattc ttatcaaatc cctagctcat ggcaagcaag tctagactga
     3661 ttaaatagag ccttcttcca tcttctcaca tcctagctct cggtaactac ggtcaccgac
     3721 aacttcaacc ctaaactgac cgtcctcata ttcaagaatc gtcacgctac cattatctag
     3781 accatgcgga tgcatgccat taatcagata aacaatggtt ccaatagtca ttccatggct
     3841 gacaacaagg gcgttacctc caccttgatc ttccatttct tttgcaatca tttcaaagcc
     3901 ttccttgatt cggccactga gtttttccca gccttcagcc caaccagctg tatcgacctc
     3961 taccaagccc tcagccagtt cagcataaga caattggtga acgtggtcca cattaaagat
     4021 acgaggaata atgcccatga aaagatcgcc atcataggct ccatcaaaac taccgaaaca
     4081 ccattctctg atacgcttgt ccatgcgata agggattttc ccctgcaagc caagttcttc
     4141 aaggataatt cccatggtct gaatggtacg accagaatca ctcgaataag cgcgctcaaa
     4201 ctgtagatca gattctcgca aaccgattcc taactcttga atccctcgtt caccttcagc
     4261 agttaaggga gtatcgctcc aaccttgcgc gcgaccaatc gtgttaaaca tggtcttgcc
     4321 atgacgtacc aaatacaatc gtacttttac cattttccgt cctccgtttg cttctattat
     4381 atcatgaatg ataaaataaa agccacccat acaggcgact tttgaaggag attattatga
     4441 aaaagtttag gagttcatta aataaagata ttagatgaaa atcaaattca aactaattca
     4501 gtgttatctg tttcaaataa tattaggagg tctttattta atgattatag tatacacatc
     4561 taatcttaaa tagaacttaa aatttctcct attttcttaa tttttaaaac tatgaattgg
     4621 tgctgggatt tgtccaccgc gagagatgaa gtcgacagac gaagccccat taaccttcat
     4681 aacaggtgca gttcctaata gaccaccaaa ctcaatcata tcgccttctc ttcctttggg
     4741 aatgatacga acagctgttg ttttcatgtt gataacaccg attgctgctt catccgcaat
     4801 catagccgca atggtttcag caggcgtatc ttctgggatg gcaatcatat ccaatccaac
     4861 agaacagata gccgtcatag cttctagttt ttctaaatta agagagccat tttgcactgc
     4921 agcaatcatt ccttcatcct cagaaacagg gataaaggcg ccagataaac caccgacttg
     4981 gttgcaggcc atcactccac cctttttaac ttggtcgttc aagagggcca aggcaaccgt
     5041 cgttccatgc gtgccaactg tttctagccc catttcctca aggacacgtg ccacagagtc
     5101 tccaatcgca ggggttggtg ccaaactcaa gtccacaata ccaaactcca cacccagtct
     5161 ctcactggcc atttgaccaa ccaattgacc gatacgagtg attttaaagg cagttttctt
     5221 aactgtttcg gctactacat caaagctctg tccacgaact ttttccaaag cacgtttcac
     5281 cacaccagga ccagaaactc cgacattgat gataacatct gcttccccaa caccatgaaa
     5341 ggcacccgcc ataaatggat tgtcctcaac agcattagcg aatacaacca acttagccgc
     5401 tcccatatct gaaagatttg ccgtttcctt gataattcgt cccatatctg ccacagccgt
     5461 catattaata ccagacttgg ttgagccgat attgactgac gagcagacct tatccgtctc
     5521 agtcaaagcg cgaggaatgg aattgatgag aatctcatct cccttttgat aacctttttg
     5581 tactaaggca gaaaaaccac caataaagtc cacaccaatc tctttcgcag ccttatcaag
     5641 cgcttttgcc agaaccacgt agtccgtcgc atctgtcgct gccccaatca gagaaatagg
     5701 tgtcaccgat acacgcttat taacgatagg aattcccaac tcagccgcaa tttcatcacc
     5761 aacagctact aaattagccg cctttgtcgt aattttttga tagattttct ccgcagcacg
     5821 attgatatct ggatcgatac agtccaaaag agaaatcccc atggtaatgg ttctaatatc
     5881 gaagttttgc tcctcaatca tggcgatggt ttcagtaact tgtctaatat ccatgatgac
     5941 ctcctagata ttatacatag cttcgaaaat cgctgcactc tgaatattga tttttacatt
     6001 caaagtttgc ccaaaagctt caaattcatt acgaagatag gtaaaatctt gcttttcatc
     6061 actagataca acagccatca tcgtaaaata ttcatccaag acagtttgag agatatcgtc
     6121 aatattcaat cctaattctg caattttacc agaaacacct gcaacaattc cagatttatc
     6181 tttaccaaca acagttataa tggctttcat aagtaaactc caattcttct taaaataaga
     6241 aaccttaaca ttaaacagga ttcttttcaa atagtatagc ataattctaa cgaaaatact
     6301 caaaaacgcc tgcttacgaa gttgttctta agaaaaaaac aatttcgtaa acaagcgtct
     6361 actatcccaa cttatgatga gtgttcccgc taggaccgaa atcctagcgg tagaccagag
     6421 ctagactaag agcacaagac tccatcatca taacactcaa caaaattgat gattttatac
     6481 taattcgatg atcgccattg gcgctgcatc accacgacgt ggttcagttt taaggatacg
     6541 agtgtatcca ccgttacgtt cagcataacg aggtgcgatt tctgagaaca atttttgaag
     6601 tgctgtagta gaagtgtact tatcagttgc ttcatcatag ttttcagatg cgatttcatt
     6661 acgtacgaaa gcagctgctt gacgacgtgc atgcaaatca ccacgtttac ctagagtaat
     6721 cattttttca acagttttac ggatttcttt agcacgagct tcagttgtca cgattgattc
     6781 gttgatcaaa aggtcagttg tcaaatcgcg aagcattgct ttacgttgtg agctagtgcg
     6841 tcctagttta cggtaagcca tgtattcctc ctttatttat cttttaatcc aagacccaaa
     6901 tcaatgagtt tgagtttcac ttcttccaaa ctcttgcgtc caagatttcg tactttcatc
     6961 atctctgctt cagatttttc tgtcaaatca tgcacagtat tgataccggc acgttttaaa
     7021 cagttgtatg aacgcacaga caagtccagt tcctcaatcg tacgatctaa aatacggtcg
     7081 tcagattcag tatcagcttc tttcatcact tcagttgact tagcaatctc agtaagattt
     7141 gtaaacaaat caagatgttc tgtcaaaata cgtgctgaaa gccctaaagc atcttctgga
     7201 ataattgttc catttgtcaa gatttcaagg gttaatttgt cgaaaccatc attgctacct
     7261 acacgagcag gttccacttg atagttgact tttgtaactg gtgtataaat agaatctaca
     7321 gcaagtgttc caactggtgc attatccttt ttattttcat cagcaggtac atatccacga
     7381 ccactgttaa cagtcatagt cgcttttaga gaagaacctt caccaattgt aaagagataa
     7441 tgatctggat ttacaatttc aatatcgcta tctgtcaaaa tgtcaccagc tgttacttca
     7501 gcaggacctt caacatccag ttcgatgatt ttttcgtctt caacgtacga tttcactgca
     7561 attcctttaa tgttcagaat gatttgcatc acgtcttcac gaacacctgg aactgtgtca
     7621 aactcatgta acacaccatc aatgttgata gatgtcacag ctgctcctgg tagagaagct
     7681 agaagtacac gacgaagaga gttaccaaga gttgtaccgt agccacgttc aagtggttcg
     7741 attacaaact tgccataatc tttattttca tcaatttttg ttatatttgg tttttcaaac
     7801 tcgatcattt agttactccc tcttaaacga aaagcagtgt aatgcgatga ttatacacgg
     7861 cgacgttttg gaggacgagc accattgtgt ggcactggag tcacatcacg aattgctgtt
     7921 acttcaagac cagcggcagc aagcgcacga atagctgact cacgaccaga acctggacct
     7981 tttacagtaa cttcaactga tttaagaccg tgttcttgtg cagatttagc agcagcttca
     8041 gaagccattt gagcagcgaa tggtgtagat ttacgagaac ctttgaaacc aagagcacca
     8101 gctgatgacc aagcaattgc attaccatgc acatcagtaa tcataacaat agtgttatta
     8161 aatgtagcgt gaatatgagc aataccagat tcgatattct ttttcacacg acgtttacgt
     8221 gttggtttag ccaagacttt tacctcctat attatttttt cttaccagca atcgcaacag
     8281 ctttaccttt acgagtgcga gcgttgtttt tagtattttg tccacggaca ggaagtccac
     8341 gacggtgacg gataccacgg tatgaaccga tttccatcaa acgtttgatg ttcaagttta
     8401 cttcacgacg aaggtcacct tcaactttga ttgcatccac ttcacgacgg atagcatctt
     8461 cttgatctga tgtaagatca cgtacacgaa catcttctga gattccagca gcagccaaaa
     8521 ttttcttaga tgttgcaagt ccgataccat aaacataagt caatgagatt actacgcgtt
     8581 tgtcatttgg aatatcaact ccagcaatac gagccatgtt ttctcctttc tatcttatcc
     8641 ttgacgttgt ttgtgttttg gatttgctgg gcaaattacc ataacacgac cattacgacg
     8701 aataacttta cagtattcgc aaattggttt gaccgatggt cttactttca tttcttatcc
     8761 ctccaagttt ttcgattatt taaagcggta agtgatacgt ccacgtgtca agtcatatgg
     8821 actcatttcg acagtaacac gatctcccgc taaaatacga atatagtttt tacgaatttt
     8881 accagaaact gttgctaaaa tctgatgtcc attttcaagt tcaaccgtaa acattgcatt
     8941 cggcattgta tcaactactt tgccttcaac ttcaatcaca tcgtcttttg ccacgcaaaa
     9001 gcacctccat aaatttcgat tcgatgcctc tagacacaga gacaacaatt ataagtcaga
     9061 ctatctcagt ataacatttg tagcggattt ttgcaagtgc gaaaaacgct ttatttcaaa
     9121 tttgtcaata cttttt
//
LOCUS       DAVBCB010000070         8830 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVBCB010000070 DAVBCB010000000
VERSION     DAVBCB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.070"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            264..1322
                     /locus_tag="VQU28_001901"
     CDS             264..1322
                     /locus_tag="VQU28_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477197.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREIALLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALGDLSKQYLNRNISELQDGSFDGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            1637..2611
                     /locus_tag="VQU28_001902"
     CDS             1637..2611
                     /locus_tag="VQU28_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477198.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGIAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSTK"
     gene            2991..3365
                     /locus_tag="VQU28_001903"
     CDS             2991..3365
                     /locus_tag="VQU28_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HET2477199.1"
                     /translation="MELVLPNNYVVLEQEEMMYLDGGFSIPRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            3573..4475
                     /locus_tag="VQU28_001904"
     CDS             3573..4475
                     /locus_tag="VQU28_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477200.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWQEKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            4531..4770
                     /locus_tag="VQU28_001905"
     CDS             4531..4770
                     /locus_tag="VQU28_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HET2477201.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINENPYPWDGFISATVR"
     gene            4767..5495
                     /locus_tag="VQU28_001906"
     CDS             4767..5495
                     /locus_tag="VQU28_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477202.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLVINSILLMSFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IRKDFDIKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGKVDNHVLTLI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKEKEYELFKNSK"
     gene            6630..6869
                     /locus_tag="VQU28_001907"
     CDS             6630..6869
                     /locus_tag="VQU28_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477203.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQDGVFILEDFRNILCTMM"
     gene            7419..>8830
                     /locus_tag="VQU28_001908"
     CDS             7419..>8830
                     /locus_tag="VQU28_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HET2477204.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPVASQSKAEK
                     DYDAAVKKYEAAKKEYEDGKAAQKKYEDDQKKTEEKAEEERKASEEEQAANLKYQQEL
                     VKYIRENDPTKKAEAKKAMDEAEKEYKKKQTEFAEVRAKVIPSAEELKKTRQKAEEAK
                     AKEAELTKKVEEAEKKVTEAKQKVDAEHAEEVAPQAKIAELEHEVQKLEKALKEIDES
                     DSEDYVKEGLRAPLQFELDVKQAKLSKLEELSDKIDELDAEIAKLEKDVEDFKNSDGE
                     QAGQYLAAAEEDLVAKKAELEKTEADLKKAVNEPEKPAEETPAPAPKPEQPAEQPKPA
                     PAPQPEKPAEEPENPAPAPQPEKSADQQAEEDYARRSEEEYNRLTQQQPPKAEKPAPA
                     PQPGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNANGAMATGWLQYNGSWYYLNAN
                     GAMATGWLQYNDSWYYLNANG"
BASE COUNT         3046 a         1215 c         1616 g         2953 t
ORIGIN      
        1 taaatctaat aaattctatt attatagctc ttggtatgat attggcatta attttggtat
       61 tatcaagagt gtatagaaaa tatttttata gagacgatga tgactaaagt ttttatcaat
      121 aatttgggct ccttgtcaac tgtagtggat tgaagaaaag ctaacgtctg gggaagggtt
      181 taaagctttg ctttatcaaa tattgactta cagagttgct ctattgtatt gatgtattct
      241 tagtaaataa tatggtgttt tgaatgaaag atgatcagaa atatttatta gcgggacttt
      301 attcgcttct agttgcaata ttttattttc cattgattga gtccaagggg atttttgtaa
      361 gtatattgat ggcggtttta cttttatatt taatatactt cattgcaaca gtcattcata
      421 ttgtgataat taagtttata agaaaaaaat cttttaagta tttggttcta tatccattta
      481 cttatgatgg gagctggaga tttcaaccta taaacttact ctactttcca gaaatggtga
      541 gagatgtcat tccgattaat ttagttcaag aatattgcca aggacaacct tacggactgt
      601 taaaaaagat gctgaaaaga attcgattgt cacgagagat agctttactc ctggctacca
      661 ttatcgtcta tttttttacg catagaattc tacctctttc agtatttacg ttcatatttt
      721 cttacatatt gctatttgca caatcttatt tgggtggtaa taccgtttgg attggaaata
      781 gaagattaat aatagatgat gaatttgaaa aaatcttgct ttcaaaaagt tatataaaag
      841 aaatatcatc tgcacgatac tcggaatact tgacatgtga atacaaaaac cttacaccaa
      901 tcatattgtt agctattttt gaaaatttgc ttgactccta cttgcttcag aatcagagtg
      961 aggttgactt agatattttt tataaagttt tacctttgct ttataaagaa aaatatacta
     1021 tgggattcaa ttactttgta agtttaaatt atttactata taaggttgga tttttgggaa
     1081 tcatttacga taatgaagca ttaggagatt tgtcaaaaca atatttaaac agaaatattt
     1141 cggaattaca agatggttct ttcgatggcg gtatacaaga tgctgttgcc tctaaacaaa
     1201 tagtagttat aaatgaattt atagcttgtt taaattccag atgcatgcca tcccaatatg
     1261 atagattttt ttataaagat agaccttata ttttttccag aaagagtcct attaaggggt
     1321 gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac tctagtatag
     1381 ctagatattt ttgagtaact agaaatatca ttaggctacc catatgattg ccagaaaaga
     1441 tggctgatga atcttattta tactgttgca gtaggcattg tctaggataa ataagctatc
     1501 ttgatggggg aaattggaat aattttagta gaaacattaa aggattatgg acaagtagcg
     1561 caggattagg tgatgcaatg tgctaagttg gcttggtgtt agcagtagta ttatttaaca
     1621 atggaaagta gactaaatga aaaataagcg ttattttttt gacacaattt taatgatttt
     1681 actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg acatcttagg
     1741 aaccccctct catgccatct taacctttgt attggcgata ccgttattct atattttgtc
     1801 tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat taagaccaat
     1861 ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc gaattctctt
     1921 tcaccaacta atgggattta gtccaggaat agcagtgttt tataaggaga gtcaaacaac
     1981 taagaatctg tttaaatttt actacttctt gtactttaca acactaattt catactattt
     2041 tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttattccat tttctattat
     2101 catagcatta gtacaaaaac tttatagaat tgagaatcaa caattatttc tgttaaaatc
     2161 aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc aggattatca
     2221 tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat cttatatcag
     2281 tttgatcaag ccatatcttt cagagagcgt gcgtgaacaa attgatttat tagaagttaa
     2341 gcgatttaaa aaaataaatc atccaatatc actttatggt atgttggatg ttataaaatt
     2401 aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt taaagaaaat
     2461 tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata gtttaaatag
     2521 ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc ttgaagtgta
     2581 tatggggatc aaacatgttt caaccaagtg ataaaaccag aataatattt gccattatag
     2641 gggcaagttg catattagtc agtatgtatc ttattccact tattaaaact gagaataaat
     2701 tttgggaaat acttttattt atattaggtg tattgctaat tggaattgcg ttactaagtc
     2761 aaaaaaaata aattttgaaa tagtattagt gagttcgcaa tcatttattg ctagtggttt
     2821 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
     2881 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
     2941 acttgttagg aaactatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
     3001 tattaccaaa taattatgtt gttcttgagc aagaagagat gatgtatctt gatgggggat
     3061 tttctattcc gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
     3121 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
     3181 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
     3241 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
     3301 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
     3361 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt tttgtatgtc
     3421 tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa ataaaaaagt
     3481 tgattatttg gattaatgtc gtttttttga ttttaatcat agcaatgatg atttatccaa
     3541 aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatt gttttcttac
     3601 taatgctccc tctttttgtc ttgtggctag agttgcatgt cctagtgaat aacttactat
     3661 tgaatctaga atctcccctt gattttgtga ttagtatgag tcttgctttc tgtagtttga
     3721 ttttatcaaa aatagtttta gacatactat acgcattgaa agatttatac aagaaagaag
     3781 gtcttattaa gatttttccc tttattttta taagaaggaa aaaagtgaat gtgagattct
     3841 cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga tctaggatta
     3901 tatggagttt tattcttgaa attgctatta tcctagtatt tattttaaaa attccttttg
     3961 caatcatcat gcttactaca atatttttct ggactataat ggatatcaat catctcgttt
     4021 ttaataagac ggaatttctt tttaatcaaa acaaatggca aaaagaagat tcatttgaaa
     4081 gtaatttgca taagatattg atggacaaaa tacaaaaatc agaattaagc tattctgact
     4141 taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg gatagtgaac
     4201 tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg tgtacaggtc
     4261 ttgtcgagtt actgctgtat gagatttcta ttagtaacaa taacaactgg caagaaaagg
     4321 ttgataaaat tagaatacag ctcatcagaa tcaatcaatt agattttttc tattatacaa
     4381 gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa atgaaatctc
     4441 ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt aaaacaaatg
     4501 aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa taattatgtt
     4561 gtgattgatg aagaagtaat gatgtatctt gatgggggag cttatttaag caagcgtgct
     4621 tgtcaaggaa tttgcgcagc agcaggtgta ttggctacag cagcagggaa aatagcttac
     4681 tgtattggat atggtgttct taatagaggt tgtgatatta acgagaaccc ttatccttgg
     4741 gatggattca tatctgcaac agtaagatga gtaatgtaga taagattcga aaaatacaca
     4801 tcatcgtgtg ttgggtgtat atatttttat cattcagggc gattataaat gatacggagt
     4861 attttttgct tattttttta gcttttatct actcaatagt atcactacct ctatattctg
     4921 tgaaaaataa aatagtatct atctgtctag ttataaattc gattctgtta atgagtttcc
     4981 ccattttaat caataagttt ttcccagaaa gttttttgac ttataccgta ttaataagtg
     5041 tttttatcct agagttagca atctttcatt taattaggaa agattttgat attaaattga
     5101 ctaacgaata taagaaaatt agtcagttta gaagcaaggt gtctcaatct ccttggataa
     5161 aatatttaga gatttctagt tttatattaa ctatatttcc atctattctt tatggtaaag
     5221 ttgataatca tgtactgact cttatctttt tgataaaaat ttgtgtagat actacgataa
     5281 aatttttatt catcagatta tttgacacaa gtactttaat gaagaggaga atattttttc
     5341 ttttcgcatt ggatgttata gcttacttat ttttaggata tcttttagtg attcaaaaag
     5401 caggctattt gttttcggtt ttacttcttt tttctaattt ttcagttcca tttatcaaag
     5461 aaaaggaata cgaattattt aaaaatagca agtaatagaa atatggatta aatcttagaa
     5521 cggcttaaaa tcagatatga taaagggaaa tagtcttatc taggtaacta agtgctacaa
     5581 acacaatcga ggatcacttg ttaggattac attttaatca tgcttaataa gaatggagaa
     5641 ttcctatggg gattgtatta ctctgaagtt tcgaatacct tagttctcgt gattttgagc
     5701 tattctagct ttagaatcct tcagaacgta aaattttaag cagtcaaaga cttagaatag
     5761 cctgaaaaca tttattttat agcaattcta ggcttagaat tgctcatata tatgtatttg
     5821 agctgctcga tagttaggac agctctattc tgagaaatct gagctatcaa tgcttcagaa
     5881 tagtatttcg ccaatggttt ttattgaaga ggtgattgtg ttatgatagt ctaaaataaa
     5941 attgatttaa tagagtaaat ggagaatcgt tacatgaaac atttttttgc aggaattggt
     6001 gagataaaac ttgttggcta cttattgtat atatgtgtag gactagcccc tctttttcat
     6061 atatatgtca taggttcgga actgagtttt gtgaaaatag tgttatctat tttgggagtt
     6121 atatttgtaa gtatgttaac tattgctcga atttatcgga cttttctata aagaatagtt
     6181 tggacttaaa ctgtatctaa aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt
     6241 gaaaagagtg agatatattg acttttgaaa aaaagatgtc ataattgagt tgggataaac
     6301 ctaaaagtaa tatagtagat tgaatctgga atagtccgtt atgactgtta aaatatttct
     6361 agaaattaat ttgactttcc taatcgattt gttcacatct tgtttcaatc tactatactt
     6421 tctcgttaaa ccgttcggca attcatggtg acatggacac atagtagtaa gcacactatg
     6481 tggcttggca gagctaaaaa ctgttccctt gcgataagcc taataagcac aacataggga
     6541 gttagagaag ccgactctaa tcaaccactt tgggcaatag tgagaactgc cccgtgcttt
     6601 tttattttga gaaaatatgg agtttgtcgt tgaaattact tgattgtatt ttggactatc
     6661 aagaaagatt caatggaaaa acatgtcaag tatcaacgaa ttataagtat ttagagattt
     6721 tcaaagtgaa tttttgtttg actgatttac atcatttatt tgacttatac aaaatcacac
     6781 gagattatgc tagtcaaaca aaacctgcta ttcaagatgg tgtttttatt ttagaagatt
     6841 ttagaaatat cctatgtaca atgatgtaat cgaaaggata tctttatgtg agtttatcgg
     6901 cgatatcttc tatatctaag ctagcaagtt gttgcatcgt agctaaggat ttatctaaaa
     6961 atactatgaa attggacgtc atattttttg aggataaaaa taaaagatcc gcagttttag
     7021 gtttacgaag agacaaaagc ggagtattta aaccagttac tctacatttt acaagcgcta
     7081 agaaatatgc taaagttcgt aaaacagatg tgaaagaaat gaaatggcta taaagattgt
     7141 ccgcaggctt aagcttgcgt tttttgttgt ttaacgaaat taaaaaacaa gctctctcat
     7201 cggaaatgtt tttataggaa tgaaggaaga tgatgctatg tttaaaaaga gaatcgaaaa
     7261 gggcaaaagt agtgtcttta tctttttgga gtagaagctt atgatataga aatttgtaat
     7321 aaaaatgtaa tataaaacat ttgacaaata tttacggggg gagtatactt aatataagta
     7381 tagtctaaaa atgactatca gaaaagaggt aaatttagat gaataagaaa aaaatgattt
     7441 taacaagtct agccagcgtc gctatcttag gggctggttt tgttacgtct caacctactt
     7501 ttgtaagagc agaagaagct cccgtagcta gtcagtctaa agctgagaaa gactatgatg
     7561 cagcagtgaa aaaatatgaa gctgctaaga aggaatatga ggacggaaaa gctgctcaga
     7621 aaaagtatga agatgatcag aagaaaactg aggagaaagc ggaagaagaa agaaaagctt
     7681 ctgaagaaga acaagctgca aatctgaaat atcaacaaga gttggttaaa tatatacgtg
     7741 aaaatgatcc aacaaaaaaa gctgaagcta agaaagcaat ggatgaggct gagaaagagt
     7801 ataagaaaaa acaaacggaa tttgctgaag ttcgtgcaaa ggtaattcct agcgcggaag
     7861 aattaaaaaa gactagacaa aaagcagaag aggctaaagc aaaagaagca gaacttacta
     7921 aaaaagtaga agaagctgag aaaaaagtta ctgaagccaa acaaaaagtg gatgcagaac
     7981 atgctgaaga agtcgctcct caagctaaaa tcgctgaatt ggaacatgaa gttcagaaac
     8041 tagaaaaagc tctcaaagag attgatgagt ctgattcaga agattatgtt aaagaaggtc
     8101 tccgtgctcc tcttcaattt gaattggatg ttaagcaagc taaactatca aaacttgaag
     8161 agttgagtga taagattgat gagttagacg ctgaaattgc aaaacttgaa aaagatgtag
     8221 aagatttcaa aaactcagac ggtgagcaag ctggacaata ccttgctgca gctgaagaag
     8281 acttagttgc taaaaaagct gaattagaaa aaactgaagc tgaccttaag aaagcagtta
     8341 atgagccaga aaaaccagct gaagaaactc cagctccagc accaaaacca gagcaaccag
     8401 ctgaacaacc aaaaccagcg ccggctcctc aaccagaaaa accagctgaa gagcctgaga
     8461 atccagctcc agcaccacaa ccagagaagt cagcagatca acaagctgaa gaagactatg
     8521 ctcgtagatc agaagaagaa tataatcgct tgacccaaca gcaaccgcca aaagcagaaa
     8581 aaccagctcc tgcaccacaa ccaggttgga aacaagaaaa cggtatgtgg tacttctaca
     8641 atactgatgg ttcaatggct acaggttggc tacaaaacaa cggttcatgg tactacctca
     8701 acgctaatgg tgctatggct acaggttggc tccaatacaa cggttcatgg tactacctca
     8761 acgctaatgg tgctatggct acaggttggc tccaatacaa cgattcatgg tattacctaa
     8821 acgctaacgg
//
LOCUS       DAVBCB010000071         8559 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVBCB010000071 DAVBCB010000000
VERSION     DAVBCB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.071"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            221..2830
                     /locus_tag="VQU28_001909"
     CDS             221..2830
                     /locus_tag="VQU28_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HET2477205.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            2863..4803
                     /locus_tag="VQU28_001910"
     CDS             2863..4803
                     /locus_tag="VQU28_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HET2477206.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            4800..6407
                     /locus_tag="VQU28_001911"
     CDS             4800..6407
                     /locus_tag="VQU28_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477207.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKPQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            6422..7162
                     /locus_tag="VQU28_001912"
     CDS             6422..7162
                     /locus_tag="VQU28_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477208.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            7165..8484
                     /locus_tag="VQU28_001913"
     CDS             7165..8484
                     /locus_tag="VQU28_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477209.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIASKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDVLIDDMNLLGLILPNFIVSIILFILIFITKSSYFIFKNAKIIS"
BASE COUNT         2949 a         1060 c         1602 g         2948 t
ORIGIN      
        1 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
       61 tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg aatgttagtg
      121 cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct gtctagctat
      181 agatatatta agtttgataa aatttcttgg agattttgtt atggattata atttcaattt
      241 ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca
      301 agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata
      361 ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa
      421 cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt
      481 taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg
      541 attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca
      601 aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg
      661 ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa
      721 gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc
      781 agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa
      841 gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa
      901 cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga
      961 ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc
     1021 ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca
     1081 cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa
     1141 tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca aagattatct
     1201 attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga
     1261 aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat
     1321 tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt
     1381 tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg
     1441 tattagacaa atagctcatt atttatacat gccactagta actacatcgg atttgacata
     1501 caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac
     1561 aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca
     1621 gattgagaat atttccagga aaaaagtttt aaaacccatg catgagatta gaactatcga
     1681 gagtgagcaa gatatacaag attttattat aaaattattg agagggttta cgctaactta
     1741 tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa
     1801 ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc
     1861 taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa
     1921 tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg
     1981 tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac
     2041 tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg ggcaggctgg
     2101 aattttactg gttggactgt attttcttag taaaggggaa attgataata aactaggaga
     2161 agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt
     2221 ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt
     2281 actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga
     2341 aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt
     2401 agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac
     2461 tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata
     2521 cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg
     2581 tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga
     2641 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
     2701 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
     2761 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
     2821 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
     2881 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
     2941 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
     3001 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
     3061 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
     3121 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
     3181 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
     3241 aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga aagttcgttc
     3301 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
     3361 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
     3421 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
     3481 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
     3541 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
     3601 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
     3661 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
     3721 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
     3781 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
     3841 gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt tttagagagg
     3901 agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt aaaaactgaa
     3961 tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt gatttggact
     4021 aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag tgaagatacg
     4081 gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta tggtaagaat
     4141 gataattctg atttagatga aattatgtta tctataatag atgcaggttt tatttatgct
     4201 atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc aggaaaggcg
     4261 ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt aagggaaaat
     4321 gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg aataattgct
     4381 ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt ttcgccattt
     4441 ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac cgaaatagca
     4501 gagtggggag atattagaga ccctgaagta aaggcttata taaagtcata ttcgcctatg
     4561 caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt gggtgagaaa
     4621 gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa ttctattgga
     4681 gttaaaagtt tgttgtattt aaacaaagca gctggtcatg gaggttttac tccatcagat
     4741 gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag gaataactta
     4801 tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt ttaatatttc
     4861 tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt agtttaaatc
     4921 agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg ggttggtcag
     4981 caatctatat atcaaattac tatttagaag taacggaagc atcaataacg aaagatataa
     5041 atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa atggttaaag
     5101 attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa gaaaactatt
     5161 ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct ctaagtttta
     5221 tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca ttgccgatta
     5281 tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac tctaacagca
     5341 atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca ttaaaatctt
     5401 attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa ctagaagatt
     5461 ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta ctaatttcag
     5521 gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat cataaaagcc
     5581 tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta gggccgatac
     5641 aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg aaaccgtttt
     5701 taaaatactt aagtggagag aagaatttta tagacgctga acatgataat aacggactgt
     5761 atacttcatc aatagatgag atacacatga aagatgttgt atattctatt acaccagaaa
     5821 ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta acaggaactt
     5881 ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag ccacaaaaag
     5941 gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca acagttgatc
     6001 agacaccata tatttttgac actactattc gtgagaacgt aactttattt caaaatgaat
     6061 atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat gaagaattag
     6121 aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg tctggaggtc
     6181 aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg tacttatttg
     6241 acgaaatatc agctaattta gataatgata attcaaattc catacatgat attctgttca
     6301 atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac aagagataca
     6361 ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg gaggcaagaa
     6421 tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt tactaggaaa
     6481 taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag gagaatttat
     6541 aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta tttctagttt
     6601 gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata aaatcagaaa
     6661 tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag catacaattt
     6721 aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat cacaaaaaaa
     6781 attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg ctggcttaaa
     6841 cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag ctagagcggt
     6901 tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag acagcgtttc
     6961 tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt gtgtaattat
     7021 ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat tgaaagatgg
     7081 aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa tcttagaggt
     7141 acaatcaact acggaggaat agagatgctc agattagttt actatcaatt tttacataat
     7201 aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt tatgggattg
     7261 gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg acttccagat
     7321 cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt ctttgtgctc
     7381 tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact tctggaggtg
     7441 ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt tatcatttcc
     7501 tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa ttattattat
     7561 tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca aacgagtgct
     7621 gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag tggttgcttt
     7681 tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt tctttcaata
     7741 gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc tttgcaacaa
     7801 attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat tgtaatctta
     7861 gatattgtga ttatttatca attatctcct tttatacagt catgttttat caaactactt
     7921 tctataatta tatttagaaa taattttatg ttcattgctt cgaagtggaa tttactatat
     7981 cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt actaatttct
     8041 agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag tgatttagag
     8101 ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact tgcaaatatt
     8161 atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca atttgaaatt
     8221 ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat atttttgaca
     8281 tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt aatatactac
     8341 tctttagagg atgttttaat agatgatatg aatcttttgg ggttaatact tccaaatttt
     8401 atagtgtcta ttattttatt tattttaata tttattacta aaagttccta ttttatcttt
     8461 aagaatgcaa aaattatttc ttaaaaattt gtaagttttc gctatataaa atagttaaat
     8521 ctattttgta tcattgcttg cattttactg gtggagaga
//
LOCUS       DAVBCB010000072         8550 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVBCB010000072 DAVBCB010000000
VERSION     DAVBCB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8550
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.072"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..128
                     /locus_tag="VQU28_001914"
     CDS             <1..128
                     /locus_tag="VQU28_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HET2477210.1"
                     /translation="FQSLNQLEQAIVDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(257..460)
                     /locus_tag="VQU28_001915"
     CDS             complement(257..460)
                     /locus_tag="VQU28_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2477211.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(453..1535)
                     /locus_tag="VQU28_001916"
     CDS             complement(453..1535)
                     /locus_tag="VQU28_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HET2477212.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(1661..2443)
                     /locus_tag="VQU28_001917"
     CDS             complement(1661..2443)
                     /locus_tag="VQU28_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HET2477213.1"
                     /translation="MVYTSLSSKDSNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2443..3786)
                     /locus_tag="VQU28_001918"
     CDS             complement(2443..3786)
                     /locus_tag="VQU28_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HET2477214.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQSL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3885..5201)
                     /locus_tag="VQU28_001919"
     CDS             complement(3885..5201)
                     /locus_tag="VQU28_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HET2477215.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYATVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKKELTAETIVINTGAVSNVLPIPGLATSKNIFDSTGIQSLDKLPEKL
                     GILGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5394..6615
                     /locus_tag="VQU28_001920"
                     /pseudo
     CDS             5394..6615
                     /locus_tag="VQU28_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
     gene            6814..8388
                     /locus_tag="VQU28_001921"
     CDS             6814..8388
                     /locus_tag="VQU28_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2477216.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2454 a         1652 c         1892 g         2552 t
ORIGIN      
        1 cgtttcagtc gcttaatcaa ttggaacaag ctattgtaga ctatattgat tactacaaca
       61 ataaacgaat taaggtaaaa ctaaaaggac ttagtcctgt gcaatacaga actaaatcct
      121 tcggataaat taattgtcta actgtttggg ggcagtacat attcaacacc tgatactatg
      181 cgttttattg tgggaagatt ttcctcattt tctagtgaaa ttgaactttt tctaacatct
      241 tttgatagat gacttcttaa tcaattatca tttccattgt tgtttcttgg ggtttcatcc
      301 cttgtctttg ataaaagcgg agagcaccct cgttatcatt ccagacactt agggttagat
      361 tattgcagcc aatttcttta gcataatagc agaaaaatga caataaaata gcccctagat
      421 attttgaaag gaaagcatag gggctatata gtttaatcaa aataaagcaa atctttgctg
      481 gtgcttgtaa agaggtcgaa gccatccttg gtaacaacac cgcagtcttc aatacgaaca
      541 ccgactttac cagggatata gatacctggt tcaacagaga agcacatgcc ttcttcgatg
      601 accatgtcgt ttccttccat gatagatggg aattcatgga catccatacc gataccatgc
      661 ccgagacggt ggttgaagta ctcaccataa ccagcttttt cgatgacctc acgggcagcg
      721 cggtccactt catgagcagt cacacctggc ttgataaagt caagagcagc ttgttgggct
      781 tcaagagtca agttgtaaat atctttcttg aattggtctg gtttgccgac agcgactgta
      841 cgagtcatat ctgacgcata gccattgacc agaacaccca ggtcaaagag gagaagagca
      901 tcattttcaa ccttattagc tgctggaatg ccgtgtggat tcgcagcatt atcaccagtc
      961 aagaccatgg tatcaaagct catttcataa ccttcacgtt tcatggcaaa gtcgatttgt
     1021 gcgatgatat ctgtctcagt cttatcaaga gaaatattgt caaaaccaac atgaacagcc
     1081 ttgtcagcat aaagacctgc aaccatcatt ttttgcactt catcagctga tttgatgagg
     1141 cgcatgcgtt ggatacgagg agtgaggttg tcaaactcag cagtctcaaa aactgttttc
     1201 aaaccatggt atttggtcaa gatgagattg tcaaactcaa cagcgacacg tttgaagtca
     1261 agttgtggaa gagcatgttt gattttttgc catggatttt cagaatcgac atagcccact
     1321 actgggaagg aaacggtgct acttgcacgt tctacttcaa gagctgggac aaagaggaga
     1381 ggttcctgat ccgctaggac aaagaggaac atttggcgtt catggggatc actgtaaaaa
     1441 ccagtgaggt aattgattgt gacggggtca gatacgacag cgacgtctag tttttctgat
     1501 tcaagatatg ttaggatttg ttgtaattta gacatatgct accttctttc tacccctcta
     1561 ttttggcaaa aaatgagaga aaatgcaagg gagaagggta aaagaaaaga caaaaaactc
     1621 cgacaagata acggagttct ttgatatcat tttcctttgt ttaagaaaag tcagccttgc
     1681 ttttgacgtc actgtactct tcagcgattt cttggctgag aatgtcctca taggcaggga
     1741 gttggatgtc ctgaccatat ttcttcttga gggcagtagt aactaggcga taagccagat
     1801 tggcattacg agagaggata ggcccgtgga agtaggaacc aaagacattc ttataatgaa
     1861 ccccttcacc gaccttttct tcgttgtttc catttccata gacaacctgt cccagcggtt
     1921 tttggtcatc agagaggaag gtacgacctt ggtgattttc aaatccatag taggtttcat
     1981 cgaaatcttc attgtgaatc ttgatgtcac cgataaaacg gttattggtc tggttgagcg
     2041 tgtagtgtcc catgacccct agcccttcga tacgttttcc tgaagcttca acataatatt
     2101 gacccaatag ttggaaacca ccgcagatag ccagaactac accgtcgttt tggatgtagt
     2161 tgtcaatgct ctctttttta gcaggtaggt cgtctgcaat gatactttgt tcaaagtctt
     2221 gaccaccacc gaaaaaggcg atgtcgtagt gattttcatc aaagtcatca tggagagaaa
     2281 cgatgtcaac ggtcacatgg gctcccagtt tttcagccac atacttgagc atgaggatgt
     2341 ttccattgtc cccgtaggta ttcatgagat ttccgtagag gtgggcaatg ttgagctgat
     2401 aggggtaatt gctatctttt gaggaaagtg aagtataaac cattagttca tctcctttct
     2461 aacaatctga cgactagcca gcagttcacg aaattccagc atggcagtat aagttgccag
     2521 aatataggca tgcttgcagt cttgattctc aatggtcttg agaacttgct ccagattact
     2581 cgtttcagtg attttctcag ctggatagcc agtcactcgg aggcgacgag cgatttcaga
     2641 atgacgaaca ccgccagcgt tgatttcagg aatgtccatg tcagtgattt gctcaaagtc
     2701 tgcatcccag atccagctag tgtcaattcc atctgcatag ttggcattaa ggaggacaga
     2761 taggctaaat ggataaggtg ctagtttgat catttcgata gcttgggttg caccgactgg
     2821 atttttaatc aagacaaggg tacattcctt gtcaccgata tgaaaggttt cttggcgtcc
     2881 aaagacagca cggctcttgt caaatccctg tttgatgagt tgggaatctg cgccgaggaa
     2941 acgggcgatg gccacagcag ctagggcgtt atagatatta tagagcccgc cgatttggat
     3001 accgtattct tggccgtcta tgacaaagcg agagcgattg ttggtcaact caaccagttt
     3061 tgtcaaacga tagtcgagat caggacgttt acatccacaa ccttcacaga tataggcacc
     3121 caagtttgca taggtattat gctcatattt gaggatgcct tggcagtcag gacagagaat
     3181 cccttcggta ttgtagtgag ccagttgggc tggtcctttt tccaagtcaa aaccaaaata
     3241 ctctataggg tttggaatag ttggcttgta gaaaagtgga ctgtctccgt taaggagaac
     3301 agtagcagtt ggaacttttc gaatggcatc caatatcatg ttataggtag tatagatttc
     3361 accgaaacgg tccatctggt cacggaagat attagtaatg acaaaaagac taggctggat
     3421 atagtcacag atacgagata gactggcttc gtcaatttcg aggacggcaa tatttttccc
     3481 agtttttgaa gatttggctg ttaggaaggt tgttgcaatc cctgtaatca tgttggcacc
     3541 gcttgggttg gttagaactt gaccataaac ctcttttaaa atgccgacag tgagggcagt
     3601 tgtcagggtt tttccatttg ttccagtgac aacgacaatc tcgtagttct tagctaggct
     3661 ttgtaaaata tctttatcaa attgaagggc gactttccct gggagcgtac ttccacgtcc
     3721 aagacggctt aaaacgaagt gggaagaacg cccagcaaga aggcccaaag tagtttttaa
     3781 gttcatgttt ctattatatc acaaaagagg gaaaagacag atttggtgtt tcgtagaagt
     3841 aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat cgcaaacaag
     3901 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
     3961 ggaatcttgt tgtccatagc aacagtaatg atgttgatga tttcttgaga accttctgag
     4021 aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt gaaagctccg
     4081 cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc tttcactgcg
     4141 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
     4201 gtgatgaaca tagtatttgg cacattgaga cggtcctcaa gtgtgtagct gccatctcca
     4261 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaaggcca
     4321 ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttgtctact
     4381 ttaatagcac cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
     4441 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
     4501 acgacaagca cttggtcacc atcgtttttg atttcagtag tatggatatt ttgaagcaat
     4561 tcaatgccat cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
     4621 ggtaggaatg tatccaaggc atctaggact gtgaccttgc ttccaagttt gttgtaaagg
     4681 ccggcaaatt caagaccgat atttccgcca ccaaggattc caagtttttc aggtaatttg
     4741 tccaagcttt ggatacctgt tgagtcaaag atgtttttgc ttgtagcaag tccaggaatt
     4801 ggcaagacgt ttgaaacagc accagtgttg atgacgattg tttcagcagt cagttctttc
     4861 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttccgcatca
     4921 aagatatcta cgcctgtacc agcaacagtc gcatagtttt taccgttgag gcgaccagtg
     4981 atcgtgtttt tagtagcaat cacttcttca aaagacaagt ccttttcagc agcaactagc
     5041 aaggttttag ttgggataca accaatgttg atacaagttc caccgtacat agccttgctg
     5101 cgttcaacga gggcaacttt tttgccagct gaagccaatt tacctgctag tgttttacca
     5161 gctttaccaa atccaataac aattaaatcg tatgttaaca tttatcttcc tcctattcga
     5221 atttcattct agcacaagaa aaaaactttt gcacaattct gctacggaaa agacagaaaa
     5281 agataaaaac gctttccaga caaaaaacta gtaatttttt gacaagcttg ataaactaaa
     5341 atcctatcta tttttatata agaaagaata aatgttcgaa aataggaggt tttatgaaat
     5401 cgaatcgtta tattattgcc tttgctgggg tcattttaca cttaatgctg ggttcgactt
     5461 atgcttggag tgtttatcgt aaccctatta ttgaaaaaac gggatgggat caggcttctg
     5521 ttgccttcgc ctttagtcta gcaatctttt gtttgggctt atcggctgca tttatggggc
     5581 gtttggtaga aaaatttggt ccgaaagtca tggggagtct atctgctttt ctatacgcag
     5641 gtggaaatat cttaacagga tttgcaatag accgtcagga actgtggttg ttgtatctag
     5701 cttatggcat tttaggtggg cttggtttgg gagcaggcta tattacccct gtgtcaacga
     5761 ttataaaatg gtttcctgat aaacgtggtc tcgcaacagg tttagcgatt atggggtttg
     5821 gttttgcttc tttattgact agtcccatag cgcaacacct catcgcaggg gtagggattg
     5881 tagaaacttt ttatatttta ggagcaagtt actttattat catgctccta gcttcacaat
     5941 tcattaagcg tccaaatgag caagagcttg caattttatc ttcttcaggg aaagaaaaaa
     6001 cagcctcttt gacgcaagga atggctgcaa atcaggctct aaaaagcaat cggttttata
     6061 tgctttggat tattttcttt atcaacatag cttgtggttt aggcttaatt tcagcggcat
     6121 cgccaatggc acaggagatg gctggcttgt ctacaagtca tgcagcagta atggtgggtg
     6181 ttttggggat tttcaatgga tttggtcgct tgctctgggc gagtttgtct gactatatcg
     6241 gtcgccctct aacctttagt atattactgc ttgtcaatct tttctctctc actttggctc
     6301 tttacagatt ccgttttatt tgtagttgct atgtctattt tgatgacttg ctatggagct
     6361 ggtttttctt tgattccagc ttatctcagt gatatttttg gaaccaagga attggccgct
     6421 ctgcatggtt atattttaac agcttgggca atggctggtt tagcgggacc tattttatta
     6481 gcagagactt ataaaatggc tcattcgtac acacaaacct tgttcgtttt tctcatttta
     6541 tacagtatcg ccttggcttt gtcttattat ctaggtcgtt caatcaaaaa agaaagtcaa
     6601 aaaccgctta catgattttc tgtagaaatg gggctatctt ttgctctggt cttgtgttat
     6661 actagatagg ttgcaaagaa aacagtactt ttcttttgtg gaaaagaagc aacacgattt
     6721 tatatctagt tatatgaaaa tacgtcataa aaagaaaagt ataaactaag ccctataggg
     6781 tggcttcgca ccacctttag aaagaagaat aacgtgaaat ttaatgaatt aaacttgtct
     6841 gctgatttgc tagcagaaat tgaaaaagct ggttttgtag aagctagtcc tatccaagaa
     6901 caaactattc ctttggccct tgaaggcaag gatgttatcg gtcaagctca gactggtaca
     6961 ggaaaaactg cagcctttgg cttgcctacc cttgaaaaaa tccgtacaga agaagcgact
     7021 atccaagctt tggtcatcgc tccaactcgt gaactagctg tccaaagtca agaagaactc
     7081 ttccgctttg gtcgtagtaa gggagtcaaa gtccgttcag tatatggcgg atcaagcatt
     7141 gaaaaacaaa ttaaggctct taaatctggt gcccatatcg tggtgggaac tccaggtcgc
     7201 ctcttggact tgattaaacg caaggccttg aaattacaag acattgaaac ccttatcctt
     7261 gacgaagcgg atgaaatgct taacatgggc ttccttgaag acatcgaagc cattatttcc
     7321 cgtgtacctg agaaccgtca aactttgctt ttctcagcaa ctatgccaga tgccatcaaa
     7381 cgtatcggtg ttcagtttat gaaagcccct gaacatgtca agattgcggc taaggaattg
     7441 acaacagaat tggttgacca gtactatatc cgtgttaagg aacaagaaaa atttgacacc
     7501 atgactcgtc tcatggatgt ggcacaacca gaactcgcta ttgtatttgg tcgtaccaaa
     7561 cgccgtgtgg atgaattgac tcgtggtttg aaaattcgtg gcttccgtgc agaaggaatt
     7621 catggcgacc tagaccaaaa caaacgtctt cgtgtccttc gtgactttaa aaatggcaat
     7681 cttgatgttt tggttgcgac agacgttgca gcgcgtggtt tggatatttc aggtgtgacc
     7741 catgtctaca actacgatat tccacaagat cctgagagtt atgttcaccg tatcggtcgt
     7801 acaggtcgtg ctggtaagtc aggtcaatct attacttttg ttgctccaaa cgaaatgggt
     7861 taccttcaaa tcattgaaaa cttgactaag aaacgcatga aaggtctcaa acctgcaagt
     7921 gtagaagaat ccttccaatc aaaaaaacag gtagctctca agaaaatcga acgtgatttt
     7981 gcagatgaaa ccattcgtgc caactttgag aaatttggta aggatgctcg caaattggct
     8041 gctgagttta ctccagaaga attggcaatg tatatcttga gtctgacagt ccaagaccca
     8101 gatagccttc cagaagtgga gattgcacgt gaaaaaccac taccgtttaa accatcaggt
     8161 aatggtttcg gtggtaaagc taagggaggt cgtggaggcc gtcgtgggga cgaccgtcga
     8221 gagcgtgatc gccgtggcaa tggtcgccgt gatgagttca aaaaaggaag tcgtggcaac
     8281 gatcgttttg ataaggaaaa acgttaccgt aaggataata aaaaaccacg caatacttta
     8341 agcgaaaagc aaacaggctt tgttattcgt aacaaaggcg ataaataaga aaaagcggtt
     8401 caaaatgaat cgctttttta tataaggaga ccgaatgtga aaagacaaat aaaattacta
     8461 tattatcttt tataatgtta tgatacacaa aaataggcaa cttgtcaata gaaaaggaac
     8521 aatttaatta aaaatattct ggtttttaat
//
LOCUS       DAVBCB010000073         8107 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVBCB010000073 DAVBCB010000000
VERSION     DAVBCB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8107
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.073"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            120..497
                     /locus_tag="VQU28_001922"
     CDS             120..497
                     /locus_tag="VQU28_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2477217.1"
                     /translation="MICQFIRDMLDLPAKNVTILEGSNIHVLPSMPYSAQDFYTSIDV
                     LAELDNGIQVIIEIQVHHQNFFINRLWAYLCSQVNQNLEKIRQREGDTHQSYKQIALV
                     YAIAIVDSNYFSDDLAFHSFIVK"
     gene            732..2123
                     /locus_tag="VQU28_001923"
     CDS             732..2123
                     /locus_tag="VQU28_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HET2477218.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIDQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            2237..3079
                     /locus_tag="VQU28_001924"
     CDS             2237..3079
                     /locus_tag="VQU28_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HET2477219.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQEQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            3352..4113
                     /locus_tag="VQU28_001925"
     CDS             3352..4113
                     /locus_tag="VQU28_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2477220.1"
                     /translation="MDNLYLVKDDSQLATFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSDFNAATQIIRESAVPAYTNNRRMVMAPDLAVWKELYLYQLMDYECSQQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWRSFLTVDKLVENLG
                     SVQAVLDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            4483..5400
                     /gene="glyQ"
                     /locus_tag="VQU28_001926"
     CDS             4483..5400
                     /gene="glyQ"
                     /locus_tag="VQU28_001926"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HET2477221.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            5660..7696
                     /locus_tag="VQU28_001927"
     CDS             5660..7696
                     /locus_tag="VQU28_001927"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HET2477222.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSELFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            7738..7995
                     /locus_tag="VQU28_001928"
     CDS             7738..7995
                     /locus_tag="VQU28_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002901318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HET2477223.1"
                     /translation="MDPKKIARINELAKKKKTEGLTPEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
BASE COUNT         2554 a         1431 c         1794 g         2328 t
ORIGIN      
        1 gctttctttt ccgaataaat agatagaagc atagaatcta gcaaacctag atttaaaaat
       61 gtgctataat agaaggagga aaaggaggat tctcagacat ccaggtatca gcccaactaa
      121 tgatttgtca atttatccgc gatatgctgg acttgccagc aaaaaatgtg acgattttgg
      181 agggaagtaa cattcacgtc ttgccttcca tgccctactc agcgcaagat ttctatacta
      241 gtatagacgt cttggcggag ttagataatg gaatccaagt tatcatcgaa attcaggttc
      301 atcatcagaa ttttttcatc aatcgcctat gggcttatct gtgcagtcag gttaatcaaa
      361 acctagaaaa aattcgccaa cgtgaaggtg atacccacca gagctacaaa caaatcgcac
      421 tagtatacgc tatcgcaatt gtcgatagta attacttctc agatgaccta gcttttcata
      481 gttttatagt aaaatgaaat gagaacagga caaatcgatc aggacagtca aatcgatttc
      541 taacaatgtt ttagaagtat aggtctacta ttctagcttc aatctactag aaattccata
      601 gatagaaaac tacataatct ctacagatac ggatgttgga gttgatgtaa gatgctttgg
      661 cttgctagag gaattgtgga ttgccaaatt gtatcattga aattattgct caaatttgtt
      721 atgatataaa tatgaataaa agtagactag gacgtggcag acacgggaaa acgagacatg
      781 tattattggc tttgattggt attttagcaa tttctatttg cctattaggc ggatttattg
      841 cttttaagat ctaccagcaa aaaagttttg agcaaaagat tgaatcgctc aaaaaagaga
      901 aagatgatca attgagtgag ggaaatcaga aggagcattt tcgtcagggg caagccgaag
      961 tgattgccta ttatcctctc caaggggaga aagtgatttc ctctgttagg gagctgataa
     1021 atcaagatgt taaggacaag ctagaaagta aggacaatct tgttttctac tatacagagc
     1081 aagaagagtc aggtttaaag ggagtcgtta atcgtaatgt gaccaaacaa atctatgatt
     1141 tagttgcttt taagattgaa gagactgaaa agaccagtct aggaaaggtt cacttaacag
     1201 aagatgggca accttttaca cttgaccaac tgttttcaga tgctagtaag gctaaggaac
     1261 agctgataaa agagttgacc tccttcatag aggataaaaa aatagatcaa gaccagagtg
     1321 agcagattgt aaaaaacttc tctgaccaag acttgtctgc atggaatttt gattacaagg
     1381 atagtcagat tatcctttat ccaagtcctg tggttgaaaa tttagaagag atagccttgc
     1441 cagtatctgc tttctttgat gttatccaat cttcgtactt actcgaaaaa gatgcggcct
     1501 tgtaccaatc ttactttgat aagaaacatc aaaaagttgt cgctctaacc tttgatgatg
     1561 gtccaaatcc agcaacgacc ccgcaggtat tagagaccct agctaaatat gatattaaag
     1621 cgactttctt tgtgcttggg aaaaatgttt ctgggaatga ggacttggtg aagaggataa
     1681 aatctgaagg tcatgttgtt ggaaaccata gttggagcca tccgattctc tcgcaactct
     1741 ctcttgatga agctaaaaag cagattactg atactgagga tgtgctaact aaagtgctgg
     1801 gttctagttc taaactcatg cgtccacctt atggtgctat tacagatgat attcgcaata
     1861 gcttggattt gagctttatc atgtgggatg tggatagtct ggactggaag agtaaaaatg
     1921 aagcatctat tttgacagaa attcagcatc aagtagctaa cggctctatc gttttgatgc
     1981 atgatattca cagtccgaca gtcaatgcct tgccaagggt cattgagtat ttgaaaaatc
     2041 aaggttatac ctttgtgacc ataccagaga tgctcaatac tcgcctaaaa gctcatgagc
     2101 tgtactatag tcgtgatgaa taagcaagaa aaaataggtc tgttagatat ttgacagact
     2161 tattttttac agaatatagt actacttaaa aaatgtttta tgctataatt gatgaataaa
     2221 atagaaggag aagcatatga atacctatca attaaataat ggagtagaaa ttccagtatt
     2281 gggatttgga acttttaagg ctaaggatgg agaagaagcc tatcgtgcag tgttagaagc
     2341 cttgaaggct ggttatcgtc atattgatac ggcggcgatt tatcagaatg aagaaagtgt
     2401 tggtcaagca atcaaagata gcggagttcc acgtgaagaa atgttcgtaa ctaccaagct
     2461 ttggaatagt cagcaaacct atgagcaaac tcgtcaagct ttggaaaaat ctatagaaaa
     2521 actgggcttg gattatttgg atttgtattt gattcattgg ccgaacccaa aaccgctcag
     2581 agaaaatgac gcatggaaaa ctcgcaatgc ggaagtttgg agagcgatgg aagacctcta
     2641 tcaagaaggg aaaatccgtg ctatcggcgt tagcaatttt cttccccatc atttggatgc
     2701 cttgcttgaa actgcaacta tcgttcctgc ggtcaatcaa gttcgcttgg cgccaggtgt
     2761 gtatcaagaa caagtcgtag cttactgtcg tgaaaaggga attttattgg aagcttgggg
     2821 gccttttggt cagggagaac tatttgatag caagcaagtg caagaaatcg cagcaaatca
     2881 cggaaaatcg attgctcaga tagccttggc ctggagcttg gcagaaggat ttttaccact
     2941 tccaaaatct gtcacaacct ctcgtattca agctaatctt gattgctttg gaattgaact
     3001 gagtcatgag gagagagaaa ccttaaaaac gattgctgtt caatcgggtg ctccacgagt
     3061 tgatgatgtg gatttctaga aaatcataaa aagaattgta cattattcta atttttgata
     3121 taatagtcag caggaaagaa agtcttatgg cgttcttcaa gcgagcttgg gatagtggga
     3181 gccaagtagg gcaaaataat gagctggcgc tttctgtagt attttcaaaa acaatgaagt
     3241 aataaattag ggtggaaccg cgtttctgac gcccctaggt taaatcaacc taggattgtc
     3301 ggacgcggtt cttttgctta ttcagtctat tgtgtgaaag aaaggagagc cgtggacaac
     3361 ctttatcttg taaaagacga tagtcaacta gctacatttc gtgattttgt agtaagaaat
     3421 actgaaaagt tgaaagatta tcaatctttt ttaaagaatg aacttgcagt ctgtgattta
     3481 ccgcaagccg ttatttggtc agattttaat gctgctacac agattattag ggaaagcgct
     3541 gttccagcct atacaaataa tagacgaatg gttatggcgc ctgatttagc tgtttggaaa
     3601 gagttgtatt tgtatcaatt gatggactac gagtgttctc agcaaactca agcaatagaa
     3661 agtcactatc attctttatc tgaaaatttc ctcttacaga ttgtaggaca tgagttagct
     3721 cattggtcgg agcatttttt agatgatttt gatggttatg actcttatat ctggttcgaa
     3781 gaggggatgg ttgaatatat tagtcgcaag tatttcttga cagaagagga atttcaagcg
     3841 gaaaaaattt gtaatcaatc tctcgtagaa ctttttcaga agaagtatgg ttggcattca
     3901 ttgaatgatt ttggttcttc gacttatgat aagaactatg caagtatttt ttatgaatac
     3961 tggcgcagct ttttgacagt agataagttg gtagaaaatt taggtagtgt acaagcggtc
     4021 ttagattctt atcatttatg ggcaaataca gaaaaaactt ttcccttgtt agattggttt
     4081 gttcagcaga aattaattga aaaagaaata taaaaactaa aggagtaaac aatgtctaag
     4141 aaattgacat ttcactgcgt cagtggcaga aacctcctta cagtcggact gccccacgct
     4201 cagcactaga gtgcctgagc tagacgcagt actaactcgt cttgcctcgt atgatcgacg
     4261 aggcagactc atgtcgcaag taattatttt ttattaagga gtaaacaatg tctaagaaat
     4321 taacatttca ctgcatcagt ggcagaaacc tccttacagt cggactgccc cacgctcagc
     4381 accctagtgc tgagctagac gcagtactaa ctcgtcttgc ctcgtataat cgacgaggca
     4441 gactcgtgtc gcaagaaatc attttttatt aaggagtaaa caatgtctaa gaaattaaca
     4501 tttcaagaaa ttattttgac tttgcaacaa ttttggaatg accaaggttg tatgcttatg
     4561 caggcttatg acaatgaaaa aggtgcgggg acaatgagtc cttacacttt ccttcgtgct
     4621 atcggacctg agccatggaa tgcagcttat gtagagccat cacgtcgtcc tgctgacggt
     4681 cgttatgggg aaaaccctaa ccgtctctac caacaccacc aattccaggt ggtcatgaag
     4741 ccttctccat caaatatcca agaactttac cttgagtctt tggaaaaatt gggaatcaat
     4801 cctttggagc acgatattcg ttttgttgag gacaactggg aaaacccatc aactggttca
     4861 gctggtcttg gttgggaagt ttggcttgac ggaatggaaa tcactcagtt cacttatttc
     4921 caacaagtcg gtggattggc aactggccct gtgactgcgg aagttaccta tggtttggag
     4981 cgcttggctt cttacattca agaagtagac tctgtctatg atatcgagtg ggctgatggt
     5041 gtaaaatacg gagaaatctt tatccagcct gagtatgagc actcaaaata ttcatttgaa
     5101 atttcggacc aagaaatgtt gcttgaaaac tttgataagt ttgaaaaaga agctggtcgt
     5161 gcattagaag aaggcttggt acaccctgcc tatgactatg ttctcaaatg ttcacatacc
     5221 tttaatctgc ttgacgcgcg tggtgccgta tctgtaacag agcgtgcagg ctatatcgct
     5281 cgtatccgta acttggcccg tgttgtagcc aaaacctttg ttgcagaacg caaacgccta
     5341 ggttacccac ttttggatga agaaacacga gttaaacttt tagcagaaga cgcagaatag
     5401 tgagtaatac tgtactctaa aatcgttaga ggcaagtcga agacttgcta gagggatatg
     5461 caccgccgta ctgttatgtg gggatttttg aaaccttagg ttcaaaaatc agtcagctaa
     5521 atccactttg atgagattgt tggaaatctt gataaattta gcattggatt tccatacaga
     5581 ctcacagagt tagttagcga cgtccccagt acctaaggtg catatcaaaa aagattgaag
     5641 aaaatgtaaa ggaaaaaaca tgacaaaaaa cttattagta gaactcggtc ttgaagaatt
     5701 accagcctat gttgtcacac caagtgaaaa acaactaggc gaaaaaatgg cagccttcct
     5761 caaggaaaac cgcctgtctt ttgaagccat tcaaaccttc tcaacaccac gtcgtttggc
     5821 tgttcgtgtg actggtttat cagacaaaca gtctgatttg acagaagatt tcaagggacc
     5881 agcaaagaaa attgccttgg atagtgatgg aaactttacc aaagcagctc aaggatttgt
     5941 ccgtggaaaa ggcttgactg ttgaagatat cgaattccgt gaaatcaagg gtgaagaata
     6001 tgtctatgtc actaaggaag aagttggtca agcagttgaa gccattgttc ctggtgttgt
     6061 agatgtcttg aagtcattga ctttccctgt cagcatgcac tgggctggaa atagctttga
     6121 atacattcgt cctgtccaca ctttaactgt tctcttggat gagcaagagt ttgacttgga
     6181 tttccttgat atcaagggaa gtcgtgtgag ccgtggccat cgtttcttgg ggaaagaaac
     6241 caaaattcag tcagcattga gctatgaaga agaccttcgt aagcagtttg taatcgcaga
     6301 tccatgtgaa cgtgagcaaa tgattgttga ccaaatcaag gaaattgagg caaaacatgg
     6361 tgtacgtatc gaaattgatg cggatttgct gaatgaagtc ttgaatttgg ttgaataccc
     6421 aactgccttc atgggaagtt ttgatgctaa ataccttgaa gttccagaag aagtcttggt
     6481 gacttctatg aaggaacacc agcgttactt tgttgttcgt gatcaagatg gaaaactctt
     6541 gccaaacttc atttctgttc gtaacggaaa cgcagagcgt ttgaaaaatg tcattaaagg
     6601 aaatgaaaaa gtcttggtag cccgcttgga agacggagaa ttcttctggc gtgaagacca
     6661 aaaattggtg atttcagatc ttgttgaaaa attaaacaat gtcaccttcc atgagaagat
     6721 tggttccctt cgtgaacaca tgattcgtac gggtcaaatc actgtacttt tggcagaaaa
     6781 agctggtttg tcagtggatg aaacagttga ccttgctcgt gcagcagcca tttacaagtt
     6841 tgacttattg acaggtatgg ttggtgaatt tgacgaactc caaggaatta tgggtgaaaa
     6901 atacaccctt cttgctggtg aaactccagc ggtggcagct gctattcgtg aacactacat
     6961 gcctacatca gctgaaggag aacttccaga gagcaaggtc ggcgcagttc tagccattgc
     7021 agacaaattg gatacgattt tgagtttctt ctcagtagga ttgattccat caggttctaa
     7081 tgacccttat gcccttcgtc gtgcaactca aggtgtggtt cgtatcttgg atgcctttgg
     7141 ttggcacatt gctatggatg agctgattga tagcctttat gcattgaaat ttgacagttt
     7201 gacttatgaa aataaagcag aggttatgga ctttatcaag gctcgtgttg ataagatgat
     7261 gggctctact ccaaaagata tcaaggaagc agttcttgca ggttcaaact ttgttgtggc
     7321 agatatgttg gaagcagcaa gtgctctcgt agaagtaagc aaggaagaag attttaaacc
     7381 atctgttgaa tcactttctc gtgcctttaa cctggctgag aaggcagaag gggttgctac
     7441 agttgattca gaactatttg agaatgacca agaaaaagct ttggcagaag cagtagaaac
     7501 actcgtttta tcaggacctg caagtcagca attgaaacaa ctctttgcgc ttagcccagt
     7561 cattgatgct ttctttgaaa atactatggt aatggctgaa gatcaggctg tccgtcaaaa
     7621 tcgtttggca atcttgtcac aactaaccaa gaaagcagct aagtttgctt gttttaacca
     7681 aattaacact aaataaaatt tgataaacgg actttatctt attacaaagg agaagaaatg
     7741 gatccgaaaa aaattgctcg tatcaatgag cttgctaaaa agaaaaaaac agaaggctta
     7801 acaccagaag aaaaagtgga acaagccaaa ctacgtgagg agtacatcga aggttatcgc
     7861 cgcgctgttc gtcaccacat tgaaggaatc aaaattgtgg acgaagaagg aaacgatgtt
     7921 acaccagaaa aactacgcca agtacaacgt gaaaaaggat tacatggccg tagtcttgat
     7981 gacccaaatt cataatatgt actctttctt ttaacaaaga tagatgaaac gataacaaag
     8041 agactagcag tttgtgtttg ctagtctttt ttcgctaaaa aaggaaccat aatggttcct
     8101 aaaaact
//
LOCUS       DAVBCB010000074         7893 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVBCB010000074 DAVBCB010000000
VERSION     DAVBCB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7893
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.074"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            101..1078
                     /locus_tag="VQU28_001929"
     CDS             101..1078
                     /locus_tag="VQU28_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HET2477224.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGVRFEAEK"
     gene            1075..2157
                     /locus_tag="VQU28_001930"
     CDS             1075..2157
                     /locus_tag="VQU28_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HET2477225.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFELLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(2221..3270)
                     /locus_tag="VQU28_001931"
                     /pseudo
     CDS             complement(2221..3270)
                     /locus_tag="VQU28_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3654..4850)
                     /gene="tuf"
                     /locus_tag="VQU28_001932"
     CDS             complement(3654..4850)
                     /gene="tuf"
                     /locus_tag="VQU28_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HET2477226.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(5761..6630)
                     /locus_tag="VQU28_001933"
     CDS             complement(5761..6630)
                     /locus_tag="VQU28_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HET2477227.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTEKPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(6853..7533)
                     /locus_tag="VQU28_001934"
     CDS             complement(6853..7533)
                     /locus_tag="VQU28_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2477228.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKVTGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEKPEIPSPQEPGTPGEPTPEKPIPQPNPEHPSVPTPNPELPNQETPTPDK
                     PTPEPGTPKAETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAGLGLGF
                     FKKKEDEK"
     gene            complement(7491..>7893)
                     /locus_tag="VQU28_001935"
     CDS             complement(7491..>7893)
                     /locus_tag="VQU28_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HET2477229.1"
                     /translation="FKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANYYKE
                     GSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPANAY
                     QKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
BASE COUNT         2299 a         1646 c         1489 g         2459 t
ORIGIN      
        1 aaagctaata gcttatttga aattttctcc tctctgttgg atagtcccgt ctatctatgt
       61 tataatgaaa gaaggattta aaatcggaaa aggagtattt atgctaaaat taggtgtcat
      121 cggaactggc gctatcagcc atcatttcat agaagcagcc cataccagtg gagaatacca
      181 actggtcgca atctattcta gaaaactaga aactgcagca acctttgctt ctcgctatca
      241 gaatatccaa ctattcgatc aattagaagt cttcttcaaa tcttcttttg atttagtcta
      301 tatcgccagt ccaaactcct tacattttgc tcaggcaaaa gctgccttgt ctgctggtaa
      361 acatgttatt cttgaaaagc cagctgtcac tcaaccacaa gaatggtttg atttgattca
      421 aacagctgaa aaaaataact gttttatctt tgaagcagct cgtaattatc acgagaaagc
      481 ctttactact atcaaaaact ttttagcaga taagcaagtg ttaggagcag atttcaacta
      541 tgccaaatac tcttccaaga tgcctgactt attggctgga caaacaccaa atgtcttttc
      601 agaccgtttt gctggtggag ccctcatgga tttggggatt tatcccctct atgctgctgt
      661 tcgtcttttt gaaaaagcta atgacgcgac ttaccatgct caacagcttg acaatagcat
      721 tgacctaaat ggagatggta tcctcttcta cccagactat caagttcaca tcaaggctgg
      781 aaaaaacatc acttccaatc ttccttgcga gatttataca acagatggaa ccttgactct
      841 caacacgatt gagcatattc gctcagctat ttttaccgac caccaaggaa atcaagtcca
      901 gcttcctatc caacaggctc ctcatacgat gactgaggaa gtcgctgcat ttgcacacat
      961 gatccagcaa ccagatctga atctctacca aacttggctg tatgatgcag gctctgttca
     1021 tgagctacta tataccatgc gccagactgc tggcgttaga tttgaggcag aaaaatgaaa
     1081 accaaactac cgactgaatg gcaagaactg agtgaccaac tcggtttcca agaattcacc
     1141 cccattcaaa ctcaactatt tgagctcctt cttgctggag aaaacctcct aggagtgagc
     1201 caaaccggaa ctggtaagac cctagcgtat ctcctaccaa gtcttctcag actacaaaag
     1261 aaaaaagccc aacaactctt gattctagca ccaaacacgg aacttgctgg acagattttt
     1321 gatgtatgta aaacgtgggc agaagctatc ggattgactg ctcaactctt cctttcaggt
     1381 tcaagtcaga aacgacaaat tgaacgtctt aaaaaggggc cagaaattct gattggaact
     1441 cctggccgta tctttgaact cattaaactt aaaaaaatca agatgatgaa tgtggaaacc
     1501 atcatcctgg atgaatttga ccaattacta gatgattctc agattcactt tgtagagaaa
     1561 atcactcact acgcacctcg tgaccaccaa ctggtctaca tgagtgccac gaccaagttt
     1621 gaccaagaaa agattgtgcc gaatacacgc actattgatc tctctgatca aaaattggac
     1681 aatatccagc atttctacat gcaggtagac caacgtcatc gagtggatat gctacgaaaa
     1741 ctggctcatg tagaggattt ccgtggtcta gtcttcttca atagcctgtc agaccttggg
     1801 aatgccgagg aaaaactaca gtatcgtgat atattggctg tttccctcgc tagtgatgtt
     1861 aatgttaaat ttagaaaaat catcttagaa aagtttaaag ataaccaact aaccctgctt
     1921 cttgcaactg accttttggc tcgtggaatt gatatcgata gcctagaatg tgtcgtaaac
     1981 tttgatattc ctagagatag cgaaacctac acacaccgtg ctggccgtac tggtcgtatg
     2041 ggcaaggaag gttatgttat cactctcgtc actcatccag aagaaatcaa aaaacttaaa
     2101 aagtttgcaa gtatccgaga aattgtccta aaaaaccaag aactctatat caaataaagc
     2161 cccattgctc tatacacatc cagtgtatgg agctttttta taagcaattc aattttatac
     2221 tcatctgctt tcaaaaagca ttctagtcca tctccgatta acgatggact ttatcacctc
     2281 cttctccaat ccttgtatga catcttgaag ttgattcatg acatcttcca aaattcgaaa
     2341 ggctttattc ttaaatccac gtttacgaat ctctttccac acttgttcaa tggggttcat
     2401 ctctggtgtg tatggaggaa taaatgcaaa accaatatta gtcggaatct ttaagatact
     2461 tgatttatgc catatagcat tgtccataac gagtaaaaaa taatcatctg gataagcttg
     2521 tgaaagcttt tctaaaaagg cgttcatcca ctcagtatta catctaccag ctattaagaa
     2581 aaatgattcg cctgtatggg catcaacagc tccataacaa tagcgaaatt ctcgtatata
     2641 gtgactatgg atatgtggac ctactcctat tggagcccaa caagatccca gtttactgat
     2701 tctaccgaaa ccagcctcat cttggtacat caagcgaacc ttacgaaaac gtctactata
     2761 tttaaaacgc tttcttgtct tcttgaattg agattttatt tttagacgcg acaatggttt
     2821 gagcgtctgc tttcttagga tgttctggac gtggcataat atttcgccaa ccatggcgct
     2881 tcaacagttg atagaaggca tcacgtgtgt aggaacgacc taactccttt ttataagcct
     2941 gaaataaggc atcaattgta acaaattctc ctgcctctgc agccttcaaa tggcgggcaa
     3001 gaaagacttt ctcttcctca actgtcatat atgcatggtt acgaccacca cgtgtttctt
     3061 gaaggagaga gtcgagtccg aactcctcat atttttttac atttcgccaa atcattgttt
     3121 gattacagtc taaaagttct ataatctctt tataagattt gcccatatca gacgaaatag
     3181 aacgatttga agacgtttat gatatttagc tgtacgagag tcttttaaaa gtgttttgat
     3241 ggtttggatt tcttctttaa ttgatttcat attactattg tataatgctt tttgatttta
     3301 gtctagtatt atactcttcg aaaatctctt caaaccgcgt cagcttcgcc ttgtcgtata
     3361 gatgttactg acttcgtcag ttctatctac aacctaaaaa cagtgttttg agcagtctgt
     3421 agctaacttt ctatagtaaa atgaaataag aacaagacaa atcgatcagg acagtcaaat
     3481 cgatttctaa caatgtttta gaagtaaatg tgtactattc tagtttcaat atattatagt
     3541 ttgctctttg attttcattg agtattagtt caatatctaa aaaaagaacc ttgccaagca
     3601 agattctttt agtgtctgac ttaaataatt gttcttctgg gaactaaatc gaattaagct
     3661 tcgatttctg taaccatacc tgaaccaaca gtacgtccac cctcacggat agagaatgta
     3721 gtaccttgtt ctacggcgat tgggtgaatc aactcaacgt cgattgtcac gttatcacca
     3781 ggcattacca tttcagtacc tgctggaagt tcgattgaac ctgtaacgtc agtagtacgg
     3841 aagtagaatt gtggacggta gttgttgaag aatggagtgt gacgtccacc ttcttcttta
     3901 gtaaggatgt agacttcacc tttgaattta gtgtgtgggt tgattgaacc tggtttagcg
     3961 ataacttgtc cacgttcgat ttcatcacgt tgaacaccac gaagaaggac acctacgtta
     4021 tctccagcaa gaccttcgtc aagttgttta cggaacattt caacaccagt aacaactgct
     4081 ttttgagttt cttctttgat accaacgatt tcgatttcgt cgttgacttt aacgatacca
     4141 cggtcgatac gtcctgaagc aactgtacca cgtccagtga ttgagaatac gtcctcgact
     4201 ggaagaagca atggtttgtc agtgtcacgt tctggttctg ggatatactc atcaactgtg
     4261 ttcatcaatt ccataacgat gtcttcgtat ttagagtcac cttcaagagc tttaagtgct
     4321 gaaccttgga taactggaag atcgtcacct gggaagtcgt attctgacaa taggtcacgg
     4381 atttccattt caaccaattc aagcaattct tcgtcgtcaa ccaagtcaac tttgttcatg
     4441 aagacgataa ggtgtttaac accaacctga cgtgaaagaa ggatgtgctc acgagtttgt
     4501 ggcattggtc cgtcagttga agctactaca aggatagctc cgtccatttg agcagcacca
     4561 gtgatcatgt ttttaacgta gtccgcgtgt cctggagcgt cgatgtgagc gtagtgacgt
     4621 ttttcagttt cgtactcaac gtgcgcagtg ttgatagtga taccgcgttc gcgttcttct
     4681 ggagcagcat cgatagacgc atagtcttta ggttggttaa ctgatgaagg caagcgacgt
     4741 gccaaaacag ttgtgatagc tgcagttagg gtagttttac cgtggtcaac gtgtccgata
     4801 gtaccaatgt taacgtgtgg tttactacga tcgtattttt cttttgccat ttgagtaaaa
     4861 gcctccaata aaatatattt tatagataga cagtaggcaa tacagtctaa ctttccttac
     4921 tattttatca aatttcagct aaattgcaag tgttttacaa cgtttttgtg aattattctt
     4981 ggtaaaaatt taactaatta atagaaagta ctaaatagac ttatccttca aggagttttc
     5041 agcattatcc atccactcac ttgctgaaaa tgtaaacagt ctctggttta tatagtaaaa
     5101 tgaaacaaga atagagcaaa tcgatcagga cagtcaaatc gatttctaac aatgttttag
     5161 aagtagatgt gtactattct agtttcaatc tactatacaa cctcaaaact gtgttttgaa
     5221 caacctgcgg ctagcttctt agtttgccct ttgattttca ttgagtataa aattgcagat
     5281 aaaacgataa caatcatagg actcagcaga gtcaatgtag gtcgaagaat agccccccta
     5341 atcgcttatt ttatagcaaa atgaaataga aactgaacat tttgttgagg aaggtaaatc
     5401 aattcctcac aatgtttgag aaagaaggga ggactattcc aacttcaacc cactataaag
     5461 cagacttaca agatagggaa cccatctccg ataatatata gtagaatgaa aactgcacaa
     5521 tttgattgga gaattcaaac caatttctaa caatgtttta gaaacaaggg tctattattt
     5581 caactgcaac ctactatagc ccattgaagc tggaatagcc cactgagact tctaaaccac
     5641 tgctggagag gagtttgttt agagtttctt tcatttccct aaaaaatcaa aaagagagaa
     5701 ccagtctgat cctctcttac ttatagatgg tgctcactca aatgttaaag gagctatttc
     5761 ttagagataa aggaatttga atacagctac tgccgcaatt gctgctgcga taggtgctac
     5821 tactggtacc caagaatacc accattttga atcgccttta tgctcaccaa gaactgattt
     5881 tggaaggaaa gcatgaagga gacgtggtcc caagtcacgg gctgggttca aggcaggtcc
     5941 tgtaggtcct ccaagtgatg ttaccaaagc cataacgagg aatccaagtg ccaagtgagc
     6001 cacagaaagt cctgaagcag tgtgtggagc cacctgtgct ttaatagcca agtcagaaaa
     6061 atcaactgtt tgtcctgctt ctattgcctt ttgtttcatg aattgaagca cttcagcacc
     6121 aaagaagttt ttagtcaaac caagagctgc aaagaacaaa acaaatgaac caacaaactc
     6181 attaatcaaa ccattgacag ttgctgcata gcgactttct tttgtaccat ggtcaatact
     6241 tgaaatagtt gagaaagttc ccaagatgtt atttggtttt tcagttttca agtagaatgg
     6301 acggtatgtt gccacaacta aggcttggcc aaagatagcc cccaagactt gcgcgataat
     6361 gtaaggtacc acttgtgccc aagggaaaag accgctaact gcaagcccta gagtgaaagc
     6421 agggttgatg tgattcccag atacgttacc aaacatcaag gctgggatca taacccccat
     6481 accataacca acagcgatga cgatccagcc actttggtga cctttcgtac ctttaagttc
     6541 aacgttggca actgcaccat tcccaagaat gatcaaaatg gcagttccca aaaattcagt
     6601 ggcatacttc agtgcccatg tgaaatccat ttgctagatt ctccttaaaa ttttttagat
     6661 attccttatt ttatcaattt ttaccctatt tagcaactga tttctggaaa tatttttaaa
     6721 aaaataggat tcatagtttc ccagttcaac gtacttgtaa gcgtttttat tcttcacgaa
     6781 agacctcatt ctcaaaacca ggaaaaagcc cacatctgtg atcttttcct ggttcctaga
     6841 ttctaaaatc tattattttt catcttcttt tttcttgaag aatcctaatc ctagaccagc
     6901 taacaaggtc atgataccag cactagccaa ggtagcatta gcttctgtac ctgtgtttgg
     6961 caattcctct ctcttacctg tctcataagt cggaacttct gggtctggat tcactggagt
     7021 ttcagctttt ggagtacctg gttctggagt tggtttatct ggtgttggag tctcttgatt
     7081 tggtagttct gggtttggtg tcggaacact tggatgctct ggatttggtt gtgggattgg
     7141 tttttctgga gtcggttcac ctggagtacc tggttcctga ggacttggaa tttctggttt
     7201 ttctggagtt ggtttatctg gtgttgataa acggtcatac cttaccgtta tttctttatc
     7261 actagagtct gacgtaactt cttgtgattc aactgttgga atatctggat ctttgtactt
     7321 gtcaatctta ccagatataa cctcgtccca gtttcctgtt gtccattcac cgtaggttac
     7381 aactcccgtg accttgttct cagtttttgt acggcttaag gttacaggtt gaacaacatc
     7441 ttcttttaca ttttggttcg taactttatc aacgtaatga atgatacgcg ttatagtctt
     7501 cgtctcagta gaggttgctg ttttgggaac cactgtttcc tcaacattct cacggtagta
     7561 atagtcaact gttgcaccgt cttctggtac gcatttgcag gagttgctac caaggtgtat
     7621 gttgttttcc ttgtgataac tcggtcttct ttgtcctcag ttgttgtttt cccttcaata
     7681 gtttttgatt ctgtggtata ctcagaacct atcgctaaat cagcttttat aacagactct
     7741 gccaacttct cttggctacc ttctttatag taattggctg ttactgtagc agtggttggc
     7801 gcttcgcttt ctctataaac taaggtcact gttctacctt cgcttacaat attcccagtt
     7861 aaacttgcag aatttgtatc tgcttcttta aaa
//
LOCUS       DAVBCB010000075         7489 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVBCB010000075 DAVBCB010000000
VERSION     DAVBCB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.075"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1038
                     /locus_tag="VQU28_001936"
     CDS             <1..1038
                     /locus_tag="VQU28_001936"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018351.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit I is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HET2477230.1"
                     /translation="IKVMDERFGHSIYIYESETLSDNQDEIPIKLTNHSLIEPFELLT
                     EMYALPKYYEKDPTPVLAPFYFTFFGMMVADLGYGLLLFLGTMLALKIFHLPSETKRF
                     LKFFNILGVAVAIWGGIYGSFFGYELPFHLISTTSDVMIILVVSVVFGFITVFAGLLA
                     SGLQKVRMKKYAEAYNSGFVWCVILLGLLFIAVGMLMPDMRLLFVLGKWVSIFNAVGI
                     LVVSIIQAKSLSGIGAGLFNLYNISSYIGDLVSFTRLMALGLSGASIASAFNLIVGLF
                     PGTLAKLTIGLVLFILLHAINIFLSLLSGYVHGARLIFVEFFGKFYEGGGKPFQPLKA
                     SEKYIKVITKN"
     gene            1054..1530
                     /locus_tag="VQU28_001937"
     CDS             1054..1530
                     /locus_tag="VQU28_001937"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HET2477231.1"
                     /translation="MEHLATYFSTYGGAFFAALGIVLAVGLSGMGSAYGVGKAGQSAA
                     ALLKEQPEKFASALILQLLPGTQGLYGFVIGILIWLQLTPELPLEKGVAYFFVALPIA
                     IVGYFSAKHQGNVAVAGMQILAKRPKEFMKGAILAAMVETYAILAFVVSFILTLRV"
     gene            1564..2145
                     /locus_tag="VQU28_001938"
     CDS             1564..2145
                     /locus_tag="VQU28_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477232.1"
                     /translation="MSNLENLRESVIEQAHEKGRMKLLDSKKKIDDEFEMQKSVIVKK
                     KEVEHERKLKELQQKYQIIFQQLKNKERQSTLVSKQKILKELFQSALIEMESWSADKE
                     MEFIYRILERYSQQEVIVTFGERTLAKFNLEQLEKLKFSFPNHLFSEQPISNESGLLI
                     SIGKIDDNYLYKTLIGSISKEESSSIANQIFIN"
     gene            2210..3217
                     /locus_tag="VQU28_001939"
     CDS             2210..3217
                     /locus_tag="VQU28_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HET2477233.1"
                     /translation="MDTNLFSKINTTISVKENDFITEDKFQKIIQSKDTETMAFILEA
                     TPYHLSIDVLENPSQTETSLMTKLVNDYRWAYAESPSNKIVTLFALRYVYHNIKVLLK
                     SKAAIKKDFSKLLIPIGIFDIESLKHLVSSLHSDTLPDFMVHEVESIWNEYETFNNIR
                     VLDVGADLAYFKHLKLLSNELDEVLSQVIVAMIDFYNIITVKRGLSQNKSHGDILQLL
                     SDEGSISAKEFIYIVENQEIFVWFNKINPSLDSIFSTYELKMQDATISSSELEFLCDL
                     LLYKILDQGRYNVDGPLVLARYLLGCEFEVKNLRMIISALQNTIPFGSIKERIRPHYG
                     S"
     gene            3207..3527
                     /locus_tag="VQU28_001940"
     CDS             3207..3527
                     /locus_tag="VQU28_001940"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HET2477234.1"
                     /translation="MEANKYKIGIIGSRDIILPFSMIGFDIFPAYQEQEAINTLRKLA
                     QSDYGVIYITEDIASMILDTIRHYDSQVVPAIILLPTHKQGLNLGLKRIEDNVEKAVG
                     HNIL"
     gene            3590..5365
                     /locus_tag="VQU28_001941"
     CDS             3590..5365
                     /locus_tag="VQU28_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HET2477235.1"
                     /translation="MTQGKIIKVSGPLVIASGMQEANIQDICRVGKLGLIGEIIEMRR
                     DQASIQVYEETSGLGPGEPVVTTGEPLSVELGPGLISQMFDGIQRPLDRFKLATHNDF
                     LVRGVEVPSLDRDIKWHFDSTIAIGQKVSTGDILGTVKETEVVNHKIMVPYGVSGEVV
                     SIASGDFTIDEVVYEIKKLDGSFYKGTLMQKWPVRKARPVSKRLIPEEPLITGQRVID
                     TFFPVTKGGAAAVPGPFGAGKTVVQHQVAKFSNVDIVIYVGCGERGNEMTDVLNEFPE
                     LIDPNTGQSIMQRTVLIANTSNMPVAAREASIYTGITMAEYFRDMGYSVAIMADSTSR
                     WAEALREMSGRLEEMPGDEGYPAYLGSRIAEYYERAGRSQVLGIPEREGTITAIGAVS
                     PPGGDISEPVTQNTLRIVKVFWGLDAPLAQRRHFPAINWLTSYSLYKDSVGIYIDGKE
                     KTDWNSKITRAMNYLQRESSLEEIVRLVGIDSLSDNERLTMEIAKQIREDYLQQNAFD
                     SVDTFTSFAKQEAMLSNILTFADQANHALELGSYFTEIMEGTVAVRDRMARSKYVSED
                     RLDEIKIISNEITHQIHLILETGGL"
     gene            5366..6751
                     /locus_tag="VQU28_001942"
     CDS             5366..6751
                     /locus_tag="VQU28_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HET2477236.1"
                     /translation="MSVIKEYRTASEVVGPLMIVEQVNNVSYNELVEIQLHNGEIRRG
                     QVLEIHEDKAMVQLFEGSSGINLEKSKIRFAGHALELAVSEDMVGRIFNGMGKPIDGG
                     PDLIPEKYLDIDGQAINPVSRDYPDEFIQTGISSIDHLNTLVRGQKLPVFSGSGLPHN
                     ELAAQIARQATVLNSDENFAVVFAAMGITFEEAEFFMEELRKTGAIDRSVLFMNLAND
                     PAIERIATPRIALTAAEYLAFEKDMHVLVIMTDMTNYCEALREVSAARREVPGRRGYP
                     GYLYTNLSTLYERAGRLVGKKGSVTQIPILTMPEDDITHPIPDLTGYITEGQIILSHE
                     LYNQGYRPPINVLSSLSRLKDKGSGEGKTRGDHAPTMNQLFAAYAQGKKVEELAVVLG
                     ESALSDVDKLYVRFTKRFEEEYINQGFYKNRNIEDTLNLGWELLSILPRTELKRIKDD
                     LLDKYLPLVEV"
     gene            6774..7385
                     /locus_tag="VQU28_001943"
     CDS             6774..7385
                     /locus_tag="VQU28_001943"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HET2477237.1"
                     /translation="MVRLNVKPTRMELNNLKERLKTAERGHKLLKDKRDELMRRFISL
                     IRENNRLRKEVESYLIDNLKSFAVAKSLKNSLMVEELFSIPSKEIELFIEKENIMSVT
                     VPRMHMNITSQNENSEYSYLSSNSEMDDVFATMNSLIDKLLRLAEVEKTCQLMADEIE
                     KTRRRVNGLEYSIIPNLSETIHYIELKLEEAERANLVRIMKVK"
BASE COUNT         2458 a         1042 c         1525 g         2464 t
ORIGIN      
        1 ataaaagtta tggatgagcg ttttggacat tctatttata tttatgaatc ggaaacattg
       61 tcggataatc aagatgaaat acctatcaaa ttaacgaatc attctttaat tgaaccattt
      121 gaattattga cagaaatgta tgctctgccc aaatattatg agaaagatcc tacacctgta
      181 ttagcaccat tttactttac attttttgga atgatggttg ctgatttagg ctatggttta
      241 ctattgtttt taggaacaat gttagcatta aaaatttttc atctaccttc agaaactaag
      301 agatttttaa aattctttaa tatattaggg gtagccgttg caatttgggg tggaatctat
      361 ggctcatttt ttggatatga gttgccattt catctgatat ctacaacctc tgatgtcatg
      421 attatattag tagtgtcagt tgtgtttggg tttattacag tatttgcagg tttgttagct
      481 tcaggactac aaaaagtaag aatgaagaaa tatgcagaag catataattc aggatttgtg
      541 tggtgtgtta ttctgcttgg cttgttattt attgctgtcg gaatgttgat gcctgatatg
      601 agactgttat ttgtattagg gaaatgggta tctattttta atgctgtggg aattttggtt
      661 gtttctatta ttcaagccaa aagcttgtca ggtattggag caggattgtt taatctatat
      721 aacatttcat cttatatagg tgatttagtt agtttcactc gattgatggc attaggatta
      781 tctggagcaa gtatagcatc agctttcaat ttaattgttg gtttgtttcc gggaacattg
      841 gctaaactaa caattggatt agtattattc attcttttac atgcgatcaa tatttttcta
      901 tcgttactat caggatatgt tcatggagca cgtctgatat ttgttgaatt ttttggtaag
      961 ttttatgagg gtggaggaaa accatttcaa cctttgaagg cttctgagaa atatattaag
     1021 gttattacaa agaattaatg gaggatatat ataatggaac atttagcaac ttatttttca
     1081 acctatggag gagctttctt cgctgcattg ggaattgtat tggcggttgg attaagtggt
     1141 atggggtctg cttatggagt tggtaaggct gggcaatctg ccgcagcttt actgaaagaa
     1201 cagccagaaa agtttgcctc tgctttgata ttgcaattac tgcccggaac acaaggatta
     1261 tatggttttg ttattggaat tttgatttgg ttgcaattaa ctccagaact tcctttagaa
     1321 aaaggcgttg cttatttctt tgtagctctt ccaattgcta ttgtaggata cttttcggct
     1381 aagcatcaag gaaatgtagc agtagcggga atgcaaatct tggctaaaag accaaaagaa
     1441 ttcatgaagg gagcaatttt ggctgccatg gtagaaacct atgcaattct tgcctttgtc
     1501 gtatcattca ttttgaccct tcgtgtataa gaaattaatt tgctattcaa aggaggtgtc
     1561 taaatgagca atttagaaaa cttacgagag tctgttattg aacaagctca tgaaaaaggg
     1621 cgtatgaaat tattggattc caaaaagaag attgatgatg aatttgaaat gcaaaagtcg
     1681 gtcattgtaa agaaaaaaga agtggaacat gaacgaaagt taaaagaatt acaacagaaa
     1741 tatcaaataa tttttcaaca attaaaaaat aaggaacgcc aatcaacgtt agtatcaaaa
     1801 cagaaaatat taaaagaact ttttcaatct gctttaatag aaatggaatc ttggagtgca
     1861 gataaagaaa tggagttcat ctatcgaatt ctggaacgat attcacaaca agaggtcata
     1921 gtaacttttg gggagcggac tttagctaaa ttcaatctgg aacaattaga gaaattgaaa
     1981 ttctcttttc caaatcattt atttagtgaa cagcctatct caaatgaatc aggcttactt
     2041 atttcaatag gtaaaattga tgataactat ttgtataaaa cattaattgg atcgatttct
     2101 aaggaagaaa gttcaagtat cgcaaatcaa atttttatca attaaggatg aaattggtta
     2161 atccttctta gaaatttgga gtattccaat aaaattagaa aggtatttta tggatactaa
     2221 tcttttttca aaaataaata cgacgatttc ggtaaaagaa aacgatttta ttacagaaga
     2281 taaatttcaa aaaattatac aatccaaaga tacggagaca atggcattta tcttagaagc
     2341 aactccctat catttatcga ttgacgtttt agaaaatcct agtcagacag agacttcgct
     2401 aatgacaaaa ttagtcaatg attatagatg ggcctatgct gaaagtccgt ctaataaaat
     2461 tgtgacttta tttgctttac gatatgttta tcataatatc aaagttttat taaaatctaa
     2521 ggcggcaatt aagaaagatt tttctaaatt attaattcca atagggattt ttgatataga
     2581 aagtttaaaa catttagttt cttccttaca ttcagataca cttcctgatt ttatggttca
     2641 tgaagtagaa tcaatttgga atgagtatga aacttttaat aatattcgtg tacttgatgt
     2701 cggagctgat ctagcatatt ttaaacatct gaaactttta tctaatgagt tagatgaggt
     2761 gctgtctcag gttattgtcg caatgattga cttttataat attattactg taaaacgtgg
     2821 tttatctcaa aataagagtc atggggatat tttacaatta ctttcagatg aaggaagtat
     2881 ttctgctaaa gaatttatat acattgtaga aaatcaagaa atatttgtgt ggttcaataa
     2941 aataaatcca agcttagatt caatcttttc aacttatgaa ttgaagatgc aggacgcaac
     3001 aatttcatct tctgagttag aatttttatg tgatttatta ttgtataaaa ttttagatca
     3061 aggaaggtac aatgtagatg ggccgttagt acttgctaga tatttattgg gatgtgagtt
     3121 tgaagtaaag aatctcagaa tgattatatc agctcttcaa aatacaattc cctttggatc
     3181 aataaaagaa aggatacgcc cgcattatgg aagctaataa gtataaaatt ggcataattg
     3241 gtagccgtga tattatttta ccctttagca tgattgggtt tgatatattt cctgcctatc
     3301 aagaacaaga agctataaat actctaagaa aattagccca atctgattat ggtgtcattt
     3361 atatcactga agacattgct tcaatgatat tagatacaat tcgccattat gattctcaag
     3421 ttgtgcctgc tattatttta ttaccgactc ataaacaagg tttaaattta ggattaaaac
     3481 gtatagagga taatgtggag aaagcagtag gacacaatat tttataataa tgtacaaaat
     3541 tgtctgtaat attattctat aatttttgga cttagtaagg agaataactt tgactcaagg
     3601 gaagattata aaagtatcgg gacctctagt tattgcatca ggtatgcagg aggctaatat
     3661 tcaagatatt tgccgtgtag gtaagctagg gttaatcggt gaaattattg aaatgagaag
     3721 agatcaggca tctatccaag tctatgagga aacatctggt cttggtccgg gagaacctgt
     3781 tgttacaact ggagaacctc tctcggttga attaggacca ggattgattt ctcaaatgtt
     3841 tgatggcata caacgcccat tagatcgatt taaattggct actcataatg attttctagt
     3901 tcgtggggta gaagttccaa gtttggatag agatattaag tggcattttg attccactat
     3961 agcaattggt caaaaagtga gtacgggtga tattctagga actgtcaagg aaaccgaggt
     4021 agttaatcat aaaattatgg ttccttatgg agtatctggc gaagtcgttt ctatcgcatc
     4081 tggcgatttt acaattgatg aagttgtata tgaaataaaa aaattggacg gtagtttcta
     4141 taaaggaacg cttatgcaaa aatggcctgt tcgcaaggcg cgtcctgttt ctaaacgttt
     4201 aatcccagaa gaaccattaa tcacaggtca acgagttatt gatacattct ttccagtaac
     4261 caaaggggga gcagcagctg ttcctggacc gtttggagca ggaaagacag ttgtacaaca
     4321 ccaagtagct aaattttcca atgttgatat tgttatttat gtcggttgtg gagaacgtgg
     4381 aaatgaaatg acggatgtac tgaatgagtt tcctgagttg attgacccta ataccggaca
     4441 atcaattatg caacggacag ttctgattgc taatacttca aatatgcctg ttgctgctcg
     4501 tgaggcttca atttatacag gaattaccat ggctgagtat tttcgtgata tgggctactc
     4561 tgtcgccatt atggctgatt cgacttcacg ttgggcagaa gcactacgtg aaatgtcagg
     4621 acgtctagaa gaaatgcctg gtgatgaggg ttatcctgct tatctgggaa gtcgtatcgc
     4681 tgaatattat gaaagagcgg gacgttctca ggttctaggg attccagaac gtgaaggaac
     4741 tattactgct attggagctg tatcgccacc cggtggagat atttcagaac cagttactca
     4801 aaacacttta cggattgtga aagttttttg ggggcttgat gctccgttgg cacagagacg
     4861 tcattttcct gcaattaact ggcttacatc ttattcactc tataaagaca gtgtgggaat
     4921 ttatatagat ggtaaagaga agacagattg gaatagtaaa ataactcgtg cgatgaacta
     4981 cttacaacgg gaatctagtt tagaggaaat tgttcgtctt gttggaattg attctctgtc
     5041 tgataatgaa cgactaacga tggaaattgc taaacaaatt cgagaagatt atttgcaaca
     5101 gaatgctttt gattcagtag atacattcac ttcgtttgca aaacaagaag caatgctaag
     5161 taatatccta acttttgctg atcaggcaaa tcatgcttta gagttaggtt cttactttac
     5221 agagattatg gaaggtaccg tggcagttcg agaccgtatg gcgcgaagta aatatgtttc
     5281 agaagataga ttagatgaaa tcaaaattat ttcaaatgag attacacatc aaattcattt
     5341 gatattagaa acaggaggtc tataaatgag tgttataaaa gaatacagaa ctgctagtga
     5401 agttgttggg cctcttatga ttgttgaaca agtaaataat gtgtcttaca atgagttagt
     5461 tgaaattcaa cttcataatg gagaaattcg tcgtggacaa gttttagaga ttcacgaaga
     5521 taaagcaatg gttcaacttt ttgaaggatc tagtggaata aatttagaaa agtctaaaat
     5581 tcgttttgct ggtcatgcac tagaattggc tgtatctgag gatatggttg gtcgtatttt
     5641 taatgggatg ggaaaaccaa ttgatggtgg accagattta attccagaga aatatttaga
     5701 tattgatggt caagctatta atcctgtatc tagagattat ccagatgaat ttattcagac
     5761 agggatctcc tctattgatc atttgaatac tcttgtacgt ggtcaaaaat taccagtatt
     5821 ttcaggttcg ggcttacctc ataatgaatt agcagctcag atagcaagac aagcgactgt
     5881 tttaaattct gatgaaaatt ttgcggttgt atttgcagca atgggtatta cttttgaaga
     5941 agctgagttt tttatggaag aactcagaaa aacaggagcg atcgatcgtt cagtcttatt
     6001 tatgaacttg gcaaatgatc ctgcaattga gcgtattgca actccccgca ttgctttaac
     6061 cgcggcagag tatctagctt ttgaaaaaga tatgcacgtt ctagttatca tgacggatat
     6121 gactaactat tgtgaagcgt tacgtgaagt ctcggcagcg cgccgtgaag ttccaggtag
     6181 acgaggctat ccgggatatt tatatacaaa tttatcaact ctatatgaaa gggctggtcg
     6241 cttagttggt aaaaaaggtt cagtgacaca gattcctatt ttaacaatgc cagaagatga
     6301 cataacacat ccaattcctg atttaactgg atacattact gaagggcaaa ttattttgtc
     6361 tcatgagttg tataatcaag gttatcgtcc accaatcaat gttttatctt ctctctctcg
     6421 attaaaagat aagggatctg gagaaggtaa aactcgtgga gatcatgctc caactatgaa
     6481 tcaactgttt gcagcctatg cccaagggaa aaaggttgaa gagttagcag tagtattagg
     6541 agaatcggct ttatctgatg tagataaatt gtatgtgaga tttacaaagc gttttgaaga
     6601 agagtacatc aaccaaggat tttataaaaa tcgaaatata gaagatacgt tgaatcttgg
     6661 gtgggaatta ctatcaattc ttcctagaac agagttaaaa cgtatcaaag atgatttgct
     6721 tgataaatat ttacctttgg tagaagttta atccagaaat ggagtgatta tctatggtac
     6781 gtttgaatgt aaaaccaact cgtatggaat tgaataactt aaaggaacgt ttgaaaacag
     6841 ctgaacgtgg acataagtta ttaaaggata aaagagatga attgatgagg cgatttattt
     6901 ctttgattcg tgagaataat cgacttcgga aagaagtaga aagttatcta attgataatc
     6961 taaaatcttt tgcagttgct aaatcattaa agaactctct aatggtggag gaattatttt
     7021 caattccatc gaaagaaatt gaattattta ttgagaaaga aaatatcatg agtgtaacag
     7081 ttcctagaat gcatatgaat attacttctc aaaatgagaa cagtgaatac agctatttat
     7141 cttctaatag tgaaatggat gatgtatttg ctacaatgaa tagtttaatt gataaattac
     7201 taagactggc agaagttgaa aaaacgtgtc agttaatggc tgatgaaata gaaaaaacac
     7261 gtagacgtgt aaatggttta gaatattcga ttattccaaa cttgtcggaa actattcatt
     7321 atatagaatt gaaactagag gaggcagaaa gagccaattt agttcgtatt atgaaagtga
     7381 agtagttcct ttatttagat ttttaattag atgaacaaat atcagcttgg ataaggcggt
     7441 aagcctttct aagctttttt aggctctttg tcaactgtag tgggttgaa
//
LOCUS       DAVBCB010000076         7474 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVBCB010000076 DAVBCB010000000
VERSION     DAVBCB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.076"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            60..929
                     /locus_tag="VQU28_001944"
     CDS             60..929
                     /locus_tag="VQU28_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2477238.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            933..1889
                     /locus_tag="VQU28_001945"
     CDS             933..1889
                     /locus_tag="VQU28_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HET2477239.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKLPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            1889..2653
                     /locus_tag="VQU28_001946"
     CDS             1889..2653
                     /locus_tag="VQU28_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477240.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            2653..3363
                     /locus_tag="VQU28_001947"
     CDS             2653..3363
                     /locus_tag="VQU28_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477241.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            3828..4484
                     /locus_tag="VQU28_001948"
     CDS             3828..4484
                     /locus_tag="VQU28_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HET2477242.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALREKFEANGIQVEEIARTSAKVL
                     "
     gene            4592..5687
                     /gene="prfB"
                     /locus_tag="VQU28_001949"
     CDS             join(4592..4663,4665..5687)
                     /gene="prfB"
                     /locus_tag="VQU28_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HET2477243.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQDKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            5705..6397
                     /gene="ftsE"
                     /locus_tag="VQU28_001950"
     CDS             5705..6397
                     /gene="ftsE"
                     /locus_tag="VQU28_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HET2477244.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            6390..7316
                     /locus_tag="VQU28_001951"
     CDS             6390..7316
                     /locus_tag="VQU28_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2477245.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
BASE COUNT         2208 a         1292 c         1682 g         2292 t
ORIGIN      
        1 agaacctatc aaaaagtgag ggaaaaccct cggaattata aatagaaaga gtgaatctta
       61 tgctccaaca actcgtaaat ggtttgattc taggtagtgt ttacgcgctg ttagccctag
      121 gatataccat ggtttacgga attatcaagc tcatcaactt cgcccatggt gatatttata
      181 tgatgggagc ctttatcggt tatttcttga tcaattcttt ccaaatgaat ttctttgtag
      241 cgcttattgt agctatgcta gcgacagcta ttcttggtgt cgtgattgag tttcttgctt
      301 accgaccttt gcgccactct actcgtattg ctgttttgat tacggctatt ggggtttctt
      361 tcctattgga gtatggaatg gtctatctgg ttggtgccaa tacccgtgcc ttccctcaag
      421 cgattcaaac agttcgatat gatttgggac caattagctt aacaaatgtg cagttaatga
      481 ttttggccat ttccttgatt ttgatgattt tgttacaagt cattgtccaa aagactaaga
      541 tggggaaagc catgcgtgca gtatcagtag atagcgacgc ggcgcaattg atggggatca
      601 atgtaaaccg tacgattagc tttaccttcg ctttgggttc tgctcttgcg ggtgcggctg
      661 gtgttctgat tgctctttat tataactctc ttgagccttt gatgggggtt actccaggtc
      721 ttaaatcttt cgttgccgca gtacttggtg gtatcggaat tattcctggt gcggctcttg
      781 gtggctttgt gattggtcta ttggaaacct ttgcgactgc ctttgggatg tcagatttcc
      841 gtgatgccat tgtttatgga atcttgttgt tgatcttgat tgtccgccca gctggtatcc
      901 ttggtaagaa tgtgaaagag aaggtgtaaa cgatgaagga aaatttaaaa gttaatattc
      961 tatggttact ccttttgtta gctggctata gcttgattag tgtactggtt tcagtcggag
     1021 tacttaatct attctatgta cagattttac aacaaattgg aattaatatt attttggctg
     1081 ttggtctcaa cttaatcgtt ggtttttcag gacaattttc acttggtcat gctggtttca
     1141 tggcgattgg tgcctatgca gcagctatta ttggttctaa attaccaacc tacggtgcct
     1201 tctttggagc tatgcttgta ggggctttgc tttcaggagc agttgcctta cttgtcggca
     1261 ttccaacctt gcgcttgaag ggggactatc ttgcggtagc aactctaggt gtttctgaaa
     1321 ttatccgtat ctttatcatc aatggtggaa gtcttacaaa tggtgcggca ggtatcttag
     1381 ggattcctaa ctttacaact tggcaaatgg tttacttctt tgtcgtgatt acaaccattg
     1441 caaccttgaa cttcttgcgt agcccaattg gccgttcaac cctctctgtt cgtgaagatg
     1501 aaatcgctgc tgagtcagtt ggggttaata cgactaaaat taaaatcatc gcttttgtct
     1561 ttggtgccat tactgcaagt attgctgggt cacttcaggc aggatttatc gggtctgttg
     1621 taccgaaaga ttacaccttc attaactcaa tcaacgtttt gattattgtt gtatttggtg
     1681 gactcggttc cattacaggt gcgattgttt cggctattgt tctgggaatt ttgaatatgc
     1741 ttctccaaga tgttgctagt gtgcgtatga ttatttacgc tttagccttg gtattggtaa
     1801 tgattttcag accaggtgga ctccttggaa cgtgggaact gagcctatca cgtttcttta
     1861 aaaaatctaa gaaggaggaa caaaactaat ggcattactt gaagtaaaac agttaaccaa
     1921 acattttggt ggtctaacag ctgttggaga tgtgactctt gaattgaacg aaggggaact
     1981 ggttggatta atcggtccaa acggagctgg gaaaaccacc cttttcaacc ttttgaccgg
     2041 tgtttatgaa ccaagcgagg gaacagtaac cctagatggt caccttttga atgggaaatc
     2101 accttataag attgcctctt tgggacttgg acgtactttc caaaatatcc gtctctttaa
     2161 agatttaaca gttttagata atgttttgat tgcttttgga aaccatcaca aacagcatgt
     2221 ttttactagt ttcttacgct taccagcttt ttacaagagt gaaaaagaat taaaggctaa
     2281 agctttggaa ttgttgaaaa tctttgattt agatggtgat gcagagactc ttgctaaaaa
     2341 tctttcctac ggacaacaac gtcgtttgga aattgttcgt gcccttgcta cggaacctaa
     2401 aattctcttc ttagatgaac cagcagcagg tatgaaccca caggaaacag ccgaattgac
     2461 tgagttaatt cgtcgtatca aagatgaatt taagattaca atcatgttga ttgaacacga
     2521 tatgaatctg gtcatggaag taacagaacg tatctacgta cttgaatatg gccgtttaat
     2581 tgctcaagga actccagacg aaattaagac caataaacgc gttatcgaag cttatctagg
     2641 aggtgaagcc taatgtctat gttaaaagtt gaaaatcttt ctgtgcatta cggtatgatc
     2701 caagcagtcc gtgatgtaag ctttgaagtt aatgaaggag aagttgtttc ccttatcggt
     2761 gccaacggtg caggtaagac aactattctt cgcaccttgt caggtttggt tcgaccaagt
     2821 tcaggaaaga ttgaattttt aggtcaagaa atccaaaaaa taccagctca gaaaatcgtg
     2881 gcaggtggtc tttcacaagt tccagaagga cgccacgtct ttcctggctt gactgttatg
     2941 gaaaatcttg aaatgggagc tttcttaaag aaaaatcgtg aagaaaatca agctaacttg
     3001 aagaaggttt tctcacgctt tcctcgtctt gaagaacgta agaaccaaga tgcagctact
     3061 ctttcaggag gggaacaaca aatgcttgcc atgggacgcg ctcttatgtc aacaccaaaa
     3121 cttcttcttt tagatgaacc atcaatggga cttgccccaa tcttcatcca agagattttt
     3181 gatatcattc aagatattca gaagcaagga acaaccgtcc tcttgattga acaaaatgcc
     3241 aataaagcac ttgcaatctc tgaccgagga tatgtattgg aaacaggaaa aatcgtccta
     3301 tcaggaacag gaaaagaact cgcttcatca gaagaagtca gaaaagcata tctaggtggc
     3361 taaaacaatc cagtggattg ttttagtcgg cagatgaaga ttacgaagta atcatcaata
     3421 tagtccgggg gcccttttta gtcggtagat tgagattgcg aagcaatcat caaatccaga
     3481 ggattgtttt agccggcaga taaagattac gaagtaatca tcaatatagt ccgggggccc
     3541 tttttagtcg gtggatgaag attgcgaagc aatcatcaaa tccagaggat tgttttagcc
     3601 ggtagattga gattgcaaac aaatctgcat ctacattgaa agcttaattt ctaataattg
     3661 aaaaaatcga atgaaaagtt tcttaccttc attcacagag ctcgatttta gagctctttt
     3721 tgctagctta ttcatacttt tctaaatttt gaaaaagaaa tgtaagcgtt tgatagattt
     3781 gcaaaaagat tgtataatag ggataagaat agaaaaggag aagtctcatg gcagttaaag
     3841 attttatgac ccgcaaggta gtttatatta gtccagatac aacagtatct catgcagcag
     3901 atttgatgag agagcaaggt ttgcaccgtc tgcctgttat cgaaaatgat caattggttg
     3961 gtttggtgac tgagggaacc attgcacaag caagtccatc taaagcaaca agtctttcta
     4021 tctatgagat gaattatctt ctgaataaga caaaagtaaa agatgtcatg attcgcgatg
     4081 ttgtcactgt ctcaggctat gctagtctag aagatgcaac ttatctgatg ttgaaaaata
     4141 agattggtat tctccctgtc gtagataacc atcaagtata cggagttatt actgaccgtg
     4201 acgttttcca agcctttctt gaaattgcag gttatggcga agaagggatt cgtgtacgct
     4261 ttgttacaga agatgaagtt ggtgttcttg gaaaaattgt ttctttgatt gtagaagaaa
     4321 atttgaatat ctcccataca gtcaatattc cgcgtaagga tggtaaggtg attatcgaag
     4381 tgcaaatcga tgaatcaatt gatttaccag ccttgagaga aaaatttgaa gcaaatggta
     4441 ttcaagtgga agaaatcgct cgcacttcag caaaagtctt gtaagaaggg aagtccaaag
     4501 gcttcttttt tcatgaaaag gggattagag caaaagatgg aaagaaatga taaaatatgc
     4561 tataatgaaa taatgtaaaa aaggagtatt tatggacatt tcagtaattc gtcagaaaat
     4621 tgacgcaaat cgtgaaaaat tagcttcttt cagggggtct ctttgacctc gaagggctag
     4681 aggaagagat tgccatcttg gaaaacaaga tgacagaacc tgatttttgg aacgataata
     4741 ttgcggccca aaaaacgtcg caagaattga atgaattaaa aaacacttac aataccttcc
     4801 ataagatgga agagttgcag gatgaagtcg aaattttatt ggattttttg gctgaagacg
     4861 agtcagtgca tgatgaactg gtagcgcagt tagccgaact tgataagata atgaccagct
     4921 acgagatgac tctactcttg tcagaacctt atgaccacaa caatgccatc ttggaaatcc
     4981 atccaggttc tggtggtact gaggcgcagg actggggtga tatgttgctt cgtatgtata
     5041 ctcgttatgg taatgctaaa ggctttaaag tggaagtgtt ggattaccaa gcaggtgatg
     5101 aggctggtat taagtcggta actttatcat ttgaagggcc taatgcctat ggtctcctca
     5161 agtcagaaat gggtgttcac cgcttagtgc gaatctcacc atttgactct gccaaacgtc
     5221 gccatacctc tttcacatct gtagaagtga tgccagaatt ggatgatact attgaagtgg
     5281 aaatccgtga agatgatatc aagatggata ccttccgttc aggtggtgcc ggtggacaaa
     5341 acgtcaataa ggtttcaaca ggtgtacgtt taacccacat tccaactgga attgttgtcc
     5401 aatcaacagt ggatcgtacc cagtatggaa atagagatcg tgccatgaag atgttgcagg
     5461 ctaagctcta tcaaatggag caagataaga aggctgcgga ggtagattct ctcaaaggtg
     5521 agaaaaagga gatcacttgg ggaagccaaa tccgttctta tgtcttcacg ccttatacta
     5581 tggtaaaaga tcaccgaact agctttgagg ttgctcaggt agataaggtt atggatgggg
     5641 acctagatgg ttttatagat gcttatctca agtggcgaat tagctaagat agaaaggaac
     5701 tcacatgtca attattgaaa tgagagatgt cgttaaaaaa tacgacaacg gaacgactgc
     5761 tctacgcggt gtttcggtca gcgttcaacc gggggaattt gcttacatcg taggaccttc
     5821 aggagcaggg aagtcaactt ttattcgttc tctgtatcgt gaagtaaaaa tcgataaagg
     5881 aagtctatca gttgctggtt ttaatctggt taagatcaaa aagaaagatg tcccgcttct
     5941 acgtcgtagt gttggggttg tcttccaaga ttataaattg ttaccaaaga aaactgtcta
     6001 tgaaaatatt gcttacgcta tggaagtaat cggggaaaat cgccgtaata tcaaaagacg
     6061 agtgatggaa gttttggact tggttggatt gaagcataag gttcgttctt tcccaaatga
     6121 actctcaggt ggggagcagc agcgaattgc gattgcgcgt gcaattgtaa ataatcccaa
     6181 agtattgata gctgatgagc caacaggaaa tctggatccg gataattcat gggaaattat
     6241 gaatctcttg gaacggatta acctacaagg gacaactatt ttgatggcga ctcataatag
     6301 ccagattgta aataccttgc gccaccgtgt cattgccatt gaaaatggcc gtgtcgttcg
     6361 tgacgaatca aaaggagagt atggatacga tgattagtag attttttcgc catttatttg
     6421 aagccttaaa aagtttgaaa cgaaatggtt ggatgacagt agctgctgtc agttcagtca
     6481 tgattacttt gaccttggtg gcaatatttg catctgttat tttcaataca gcgaaactag
     6541 ctacagatat tgaaaataat gtccgtgtag tagtttatat ccgaaaggat gtggaagata
     6601 atagtcagac aattgaaaaa gaaggtcaaa ctgttacaaa taatgactac cacaaggtat
     6661 atgattcttt gaagaacatg tctacggtta aaagtgttac cttttcaagt aaagaagaac
     6721 aatatgaaaa attaaccgag ataatgggag ataactggaa aatctttgaa ggagatgcca
     6781 atcctctcta tgatgcctat attgtagagg caaacactcc aaatgatgta aaaactatag
     6841 ccgaagatgc taaaaaaatt gaaggtgtct ctgaggttca agatggcggt gccaatacag
     6901 aaagactctt caagttagct tcatttatcc gtgtttgggg actagggatt gctgctttgt
     6961 taatttttat cgcagttttc ttgatttcaa ataccattcg tattaccatt atttcccgca
     7021 gtcgcgaaat tcaaatcatg cgcttggttg gagctaaaaa cagttatatc cgtggaccgt
     7081 tcttgttaga aggagccttt atcggtttat tgggagctat cgcaccatct gttttggtct
     7141 ttattgttta tcaaattgtt taccaatctg tcaacaaatc gttggtaggg caaaatctat
     7201 ccatgattag tccagattta tttagtccgt tgatgattgc cctactattt gtgattgggg
     7261 ttttcattgg ttcattggga tcaggaatat ccatgcgccg attcttgaag atttaggtaa
     7321 aatagctgct tttatgagga gattgtaaaa tctccttttt tgctacaaga gtttttgaaa
     7381 agagatgcgt agaagaaaag agcttccaaa gaagtccccc agagaagact tcttgatggc
     7441 taatttgaac ttgaaatttg gcgcactaga aatt
//
LOCUS       DAVBCB010000077         7395 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVBCB010000077 DAVBCB010000000
VERSION     DAVBCB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7395
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.077"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            226..6657
                     /locus_tag="VQU28_001952"
     CDS             226..6657
                     /locus_tag="VQU28_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HET2477246.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALNISDKEKV
                     VVDKETENKEKHEDIHNAIETSQDFKEKKTAVIEGKEVVSKNPVTDNNTSNEEARIKE
                     DSNQSQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKQLSNLKNTKVLYTY
                     DRIFNGSAIETTPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSIN
                     APFGKNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKI
                     PHAFNYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQ
                     IFSYKMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRAL
                     RKAGIPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQT
                     VEFDKVNIGGESFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDL
                     RGKIAVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGT
                     KSQVFSISGDDGVKLWNMINPNKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVG
                     DEKEIDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVIN
                     GKSTYGYMSGTSMATPIVAASTVLIRPKLKEMLERPVLKNLKGDDKIDLTSLTKIALQ
                     NTARPMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGS
                     ISLKEIKGDKKYFTIKLHNTSNRPLTFKVSASAITTDSLTDRLKLDETYKDEKSPYGK
                     QIVPEIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESV
                     EEMEALNSNGKKINFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSRSKTLGGYDDDGK
                     PKIPGTLNKGIGGEHGIDKFNPAGVIQNRKDKNTTSLDQNPELFAFNNQGINAPSSSG
                     SKIANIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQRDLKVIS
                     REHFIRGILNSKSNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATK
                     IRGQFEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLIT
                     KDTYHKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYA
                     GEGQGRNRKLDKDGNTIYEIKGAGDLRGKIIEVIALDGSSNFTKIHRIKFANHADEKG
                     MISYYLVDPDKDASKYEKLGEISEDKLKNAKSPEENTNNNQAKDEDSKPDEKSSVEGE
                     ASLEINKTISTIREFENKDLKKLIKKKFREVDDFTSETGKRIEEYDYKYDDKGNIIAY
                     DDGSALQYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNSYKSIEI
                     KATKYDSHSKTMIFDLYANINDIVDGLAFAGDMRLFVKDDNQIKAETKIRMPEKNKET
                     KAEYPYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGY
                     ALKVTTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQ
                     AVLMSALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKS
                     NVEAIKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWE
                     LRVNESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDG
                     NVYLQTGYSDLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRN
                     NGFDGEIQEQHIYVNGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTG
                     EVSELKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKD
                     AGYVINLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQ
                     REDHSQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVG
                     IFLGLKKKNQD"
     gene            complement(6948..7294)
                     /locus_tag="VQU28_001953"
                     /pseudo
     CDS             complement(6948..7294)
                     /locus_tag="VQU28_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632353.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2880 a         1001 c         1431 g         2083 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaaacatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa catgaagata ttcataatgc tatagaaact
      421 tcacaggatt ttaaagagaa gaaaacagca gtcattgagg gaaaagaagt tgttagtaaa
      481 aatcctgtga cagacaataa cactagcaat gaagaagcaa gaatcaaaga agattccaat
      541 caatcccaag gagatcatac ggactcattt gtgaataaaa acacagaaaa tcccaaaaaa
      601 gaagataaag ttgtctatat tgctgaattt aaagataaag aatctggaga aaaagcaatc
      661 aagcaactat ccaatcttaa gaatacaaaa gttttatata cttatgatag aatttttaac
      721 ggtagtgcca tagaaacaac tccagataac ttggacaaaa ttaaacaaat agaaggtatt
      781 tcatcggttg aaagggcaca aaaagtccaa cccatgatga atcatgccag aaaggaaatt
      841 ggagttgagg aagctattga ttacctaaag tctatcaatg ctccgtttgg gaaaaatttt
      901 gatggtagag gtatggtcat ttcaaatatc gatactggaa cagattatag acataaggct
      961 atgagaatcg atgatgatgc caaagcctca atgagattta aaaaagaaga cttaaaagga
     1021 actgataaaa attattggtt gagtgataaa atccctcatg cgttcaatta ttataatggt
     1081 ggcaaaatca ctgtagaaaa atatgatgat ggaagggatt attttgaccc acatgggatg
     1141 catattgcag ggattcttgc tggaaatgat actgaacaag atatcaaaaa ctttaacggc
     1201 atagatggaa ttgcacctaa tgcacaaatt ttctcttaca aaatgtattc tgacgcagga
     1261 tctgggtttg cgggtgatga aacaatgttt catgctattg aagattctat caaacacaac
     1321 gttgatgttg tttcggtatc atctggtttt acaggaacag gtcttgtagg tgagaaatat
     1381 tggcaagcta ttcgggcatt aagaaaagca ggcattccaa tggttgtcgc tacgggtaac
     1441 tatgcgactt ctgcttcaag ttcttcatgg gatttagtag caaataatca tctgaaaatg
     1501 accgacactg gaaatgtaac acgaactgca gcacatgaag atgcgatagc ggtcgcttct
     1561 gctaaaaatc aaacagttga gtttgataaa gttaacatag gtggagaaag ttttaaatac
     1621 agaaatatag gggccttttt cgataagaat aaaatcacaa caaatgaaga tggaacaaaa
     1681 gctcctagta aattaaaatt tgtatatata ggcaaggggc aagaccaaga tttgataggt
     1741 ttggatctta ggggcaaaat tgcagtaatg gatagaattt atacaaagga tttaaaaaat
     1801 gcttttaaaa aagctatgga taagggtgca cgcgccatta tggttgtaaa tactgtaaat
     1861 tactacaata gagataattg gacagagctt ccagctatgg gatatgaagc ggatgaaggt
     1921 actaaaagtc aagtgttttc aatttcagga gatgatggtg taaagctatg gaacatgatt
     1981 aatcctaata aaaaaactga agtcaaaaga aataataaag aagattttaa agataaattg
     2041 gagcaatact atccaattga tatggaaagt tttaattcca acaaaccgaa tgtaggtgac
     2101 gaaaaagaga ttgactttaa gtttgcacct gacacagaca aagaactcta taaagaagat
     2161 atcatcgttc cagcaggatc tacatcttgg gggccaagaa tagatttact tttaaaaccc
     2221 gatgtttcag cacctggtaa aaatattaaa tccacgctta atgttattaa tggcaaatca
     2281 acttatggct atatgtcagg aactagtatg gcgactccaa tcgtggcagc ttctactgtt
     2341 ttgattagac cgaaattaaa ggaaatgctt gaaagacctg tattgaaaaa tcttaaggga
     2401 gatgacaaaa tagatcttac aagtcttaca aaaattgccc tacaaaatac tgcgcgacct
     2461 atgatggatg caacttcttg gaaagaaaaa agtcaatact ttgcatcacc tagacaacag
     2521 ggagcaggcc taattaatgt ggccaatgct ttgagaaatg aagttgtagc aactttcaaa
     2581 aacacggatt ctaaaggttt ggtaaactca tatggttcca tttctcttaa agaaataaaa
     2641 ggtgataaaa aatactttac aatcaagctt cacaatacat caaacagacc tttgactttt
     2701 aaagtttcag catcagcgat aactacagat tctctaactg acagattaaa acttgatgaa
     2761 acatataaag atgaaaaatc tccatatggt aagcaaattg ttccagaaat tcacccagaa
     2821 aaagtcaaag gagcaaatat cacatttgag catgatactt tcactatagg cgcaaattct
     2881 agctttgatt tgaatgcggt tataaatgtt ggagaggcca aaaacaaaaa taaatttgta
     2941 gaatcattta ttcattttga gtcagtggaa gaaatggaag ctctaaactc caacgggaag
     3001 aaaataaact tccaaccttc tttgtcgatg cctctaatgg gatttgctgg gaattggaac
     3061 cacgaaccaa tccttgataa atgggcttgg gaagaagggt caagatcaaa aacactggga
     3121 ggttatgatg atgatggtaa accgaaaatt ccaggaacct taaataaggg aattggtgga
     3181 gaacatggta tagataaatt taatccagca ggagttatac aaaatagaaa agataaaaat
     3241 acaacatccc tggatcaaaa tccagaatta tttgctttca ataaccaagg gatcaacgct
     3301 ccatcatcaa gtggttctaa gattgctaac atttatcctt tagattcaaa tggaaatcct
     3361 caagatgctc aacttgaaag aggattaaca ccttctccac ttgtattaag aagtgcagaa
     3421 gaaggattga tttcaatagt aaatacaaat aaagagggag aaaatcaaag agacttaaaa
     3481 gtcatttcga gagaacactt tattagagga attttaaatt ctaaaagcaa tgatgcaaag
     3541 ggaatcaaat catctaaact aaaagtttgg ggtgacttga agtgggatgg actcatctat
     3601 aatcctagag gtagagaaga aaatgcacca gaaagtaagg ataatcaaga tcctgctact
     3661 aagataagag gtcaatttga accgattgcg gaaggtcaat atttctataa atttaaatat
     3721 agattaacta aagattaccc atggcaggtt tcctatattc ctgtaaaaat tgataacacc
     3781 gcccctaaga ttgtttcggt tgatttttca aatcctgaaa aaattaagtt gattacaaag
     3841 gatacttatc ataaggtaaa agatcagtat aagaatgaaa cgctatttgc gagagatcaa
     3901 aaagaacatc ctgaaaaatt tgacgagatt gcgaacgaag tttggtatgc tggcgccgct
     3961 cttgttaatg aagatggaga ggttgaaaaa aatcttgaag taacttacgc aggtgagggt
     4021 caaggaagaa atagaaaact tgataaagac ggaaatacca tttatgaaat taaaggtgcg
     4081 ggagatttaa ggggaaaaat cattgaagtc attgcattag atggttctag caatttcaca
     4141 aagattcata gaattaaatt tgctaatcat gctgatgaaa aggggatgat ttcttattac
     4201 ctagtagatc ctgacaaaga tgcttctaaa tatgaaaaac ttggtgaaat ttctgaagat
     4261 aaactcaaaa atgcaaagag cccagaggaa aataccaata ataatcaagc taaggatgaa
     4321 gattcaaaac cagatgaaaa aagttcagtt gagggagaag ctagccttga aataaataaa
     4381 actatttcaa caattagaga gtttgaaaat aaagacctaa agaaactcat taaaaagaaa
     4441 tttagagaag ttgatgattt tacaagtgaa actggtaaga gaatagagga atacgattat
     4501 aaatacgatg ataagggaaa tattattgct tatgacgatg gtagtgcctt acaatatgaa
     4561 actgaaaaac ttgacgaaat aaaatcaaaa atttatggtg ttctaagccc atctaaagat
     4621 ggacactttg aaattcttgg aaagataagc aatgtttcta aaaatgccaa ggtatattat
     4681 ggaaatagct ataaatcgat agaaatcaaa gcgactaagt atgattcaca ttcaaaaacg
     4741 atgatatttg atttatacgc taatattaat gatattgtag atggattagc ttttgctgga
     4801 gatatgagat tatttgttaa agatgacaat caaataaaag ctgaaactaa gattagaatg
     4861 cctgaaaaaa ataaggaaac taaagcagaa tatccctatg tatcaagtta tgggaatgta
     4921 atcgaattag gagaaggaga tctttcaaag aacaaaccag acaatttaac taaaatggaa
     4981 tctggtaaaa tctattctga ttcagaaaaa caacaatatc tgttaaagga taacatcatt
     5041 ctaagaaaag gctatgcact aaaagtgact acctataatc ctggaaaaac ggatatgtta
     5101 gaaggaaatg gagtctatag caaggaagat atagcaaaaa tacaaaaggc caatcctaat
     5161 ctaagagtcc tttcagaaac aacaatttat gctgatagta gaaatgttga agatggaaga
     5221 agtacccaag ctgtattaat gtcggctttg gacggcttta acattataag gtatcaagtg
     5281 tttacattta aaatgaacga taaaggggaa gctatcgata aagacggaaa tcttgtgaca
     5341 gattcttcta aacttgtatt atttggtaag gatgataaag aatacactgg agaggataag
     5401 tccaatgtag aagctataaa agaagatggc tccatgttat ttattgatac caaaccagta
     5461 aacctttcaa tggataagaa ctactttaat ccatctaaat ctaataaaat ttatgtacga
     5521 aatccagaat tttatttaag aggtaagatt tctgataagg gtggttttaa ctgggaattg
     5581 agagttaatg aatcggttgt agataattat ttaatctacg gagatttaca cattgataac
     5641 actagagatt ttaatattaa gctgaatgtt aaagacggtg acatcatgga ctggggaatg
     5701 aaagactata aagcaaacgg atttccagat aaggtaacag atatggatgg aaatgtttat
     5761 cttcaaactg gctatagcga tttgaatgct aaagcagttg gagtccacta tcagttttta
     5821 tatgataatg ttaaacccga agtaaacatt gatcctaagg gaaatactag tatcgaatat
     5881 gctgatggaa aatctgtagt ctttaacatc aatgataaaa gaaataatgg attcgatggt
     5941 gagattcaag aacaacatat ttatgtaaat ggaaaagaat atacatcatt tgatgatatt
     6001 aaacaaataa cagacaagac actaaacatt aagattgttg taaaagattt tgcaagaaat
     6061 acaaccgtaa aagaattcat tttaaataaa gatacgggag aggtaagtga attaaaacct
     6121 catagggtaa ctgtgaccat tcaaaatgga aaagaaatga gttcaacgat agtgtcggaa
     6181 gaagatttta ttttacctgt ttataagggt gaattagaaa aaggatacca atttgatggt
     6241 tgggaaattt ctggtttcga aggtaaaaaa gacgctggct atgttattaa tctatcaaaa
     6301 gataccttta taaaacctgt attcaagaaa atagaggaga aaaaggagga agaaaataaa
     6361 cctacttttg atgtatcgaa aaagaaagat aacccacaag taaaccatag tcaattaaat
     6421 gaaagtcaca gaaaagagga tttacaaaga gaagatcatt cacaaaaatc tgattcaact
     6481 aaggatgtta cagctacagt tcttgataaa aacaatatca gtagtaaatc aactactaac
     6541 aatcctaata agttgccaaa aactggaaca gcaagcggag cccagacact attagctgcc
     6601 ggaataatgt ttatagtagg aatttttctt ggattgaaga aaaaaaatca agattaagac
     6661 aaaagctata gaaaaaaatg gtttatgtac tgagattaga tagtgaggtg atgacatagt
     6721 tttgtgaaaa cagccattta taactcaatt atttagttta cttcactatt gatactattt
     6781 ggagttatta atggacttag tttagataac taatgaattg attgaaaggg ttagtattga
     6841 caatattggt catattgatt agaaaataga gtctatcaaa atttaaaggc taatagaggt
     6901 gatgagacaa tttcggctct ttgtcaactg tagtgggttg aagtcagcta agctcgagaa
     6961 aggacaaatt ttgtcctttc ttttttgata ttcagagcga tgaaaatccg ttttttgaag
     7021 ttttcaaagt ttcgaaaacc aaaggcattg cgcttgataa gtttgatgag attattggtc
     7081 gcttccagtt tggcattaga atagtgtagt tgaagggcat tgacaatctt ctctttatct
     7141 ttgaggaagg ttttagagga tgaacctgat tcagattgtc ctcaatgagt ccgaaaaatt
     7201 tgtcaggctc cttattctga aagtgaaaaa gcaagagttg atagagatta tagtggtgtt
     7261 tcaagtcttc tgaatagctc aaaagtttat ctatagtaga ttgaaactag aatagtacac
     7321 ctctgcttct aaaacattgt tagaaatcga tttgactgtc ctgaatgatt tgtcctgtta
     7381 ttatttcatt ttact
//
LOCUS       DAVBCB010000078         7220 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVBCB010000078 DAVBCB010000000
VERSION     DAVBCB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.078"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            231..645
                     /locus_tag="VQU28_001954"
                     /pseudo
     CDS             231..645
                     /locus_tag="VQU28_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720030.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(684..1127)
                     /locus_tag="VQU28_001955"
     CDS             complement(684..1127)
                     /locus_tag="VQU28_001955"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HET2477247.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(1157..3379)
                     /locus_tag="VQU28_001956"
     CDS             complement(1157..3379)
                     /locus_tag="VQU28_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HET2477248.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRMIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            3790..4032
                     /locus_tag="VQU28_001957"
     CDS             3790..4032
                     /locus_tag="VQU28_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477249.1"
                     /translation="MTRFEEIFEEYKNPQDTFFYPLVYKENTYEKTAISIFALLMLGV
                     CCLFLFSQQSYKKLVQYYANDQNLPSRITYSEYSDK"
     gene            4372..5001
                     /locus_tag="VQU28_001958"
     CDS             4372..5001
                     /locus_tag="VQU28_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HET2477250.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            complement(5124..7016)
                     /gene="pepO"
                     /locus_tag="VQU28_001959"
     CDS             complement(5124..7016)
                     /gene="pepO"
                     /locus_tag="VQU28_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HET2477251.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         1985 a         1681 c         1378 g         2176 t
ORIGIN      
        1 ttatcatttt atgacaaaca tactatcgtt aaatgctgat ttaatcgaaa ttttgagtta
       61 taaactcctc agtttcgaac tcaaaattag ccgtgcagga taggataccc ttcataaact
      121 tatataacta ctttaatacc ttaaaaagct aataaaataa ggaatatcta agcaatatac
      181 attctaaaat acctgtacgg gagtgtctct aaagaaaagg aggttttagc atgaaaaaaa
      241 tcatcttcat caaaaccatt caactccttg tcattgatgg aatcatgctg gcatttttga
      301 catttaaaag gggggcttac ttgggactgg attttgattt atagtggttg gctcattttc
      361 tttcatcctg tgctattgac ctatctttca aaccaacttt gtgaccactt tagtcaactc
      421 tattcccaga ttagaccgag attctggcgt tttgctttac aaattctcct atgggatagc
      481 ctgatgattc tctccttggt gtctttaagt gatattccac ttttccttca gggaactctc
      541 ctcatcctag gacatctcat cccttcctat cgcatctgcc aaagcctgaa aagagacttc
      601 ccccaagcat atcaagaacc gatttctttt tggagtattt tatgatcgat gagaaagacc
      661 aagccgactg ggcttggtct ttcttatctc tttttagtat ctaggataat ggtaacaggt
      721 ccgttattaa ccagctcaac ctgcatatct gctccaaaga tacctgtctg aacgggcact
      781 tcttgcgcta atttttgatt gaaagcatca tagaagtctg atgccatatc aggtttagct
      841 gcccctgtaa aggctggacg attgcctttc ttagtatccg caaagagggt aaactgagaa
      901 atagagagga tttctccttc aatatctttg acagacaggt tcatcttgcc ttctgcgtct
      961 gaaaaaatcc gcatattgac cagttttctc acagcatagt ccaaatcttc ctcttggtcc
     1021 tctggtccaa caccaaccag caataaaagt ccctgattga tttttccctg aatctggcct
     1081 tctatactca cttgggcttt tttaacccgt tggataatga ttttcataat agcctttcta
     1141 gtaagagcta ggacaactag ccgttggtcc gtttgacaga gtaaacttct ggcacactct
     1201 taattttatc gacaaccgtg gtcagtgtag agaggttggc aataccgaag gacacatgga
     1261 tattagcaaa cttcatatcc ttggttggtt gggcattgac cgttgaaata ttcttggttg
     1321 tatttgaaag aacttgcagt acatcgttca acagtcctgt acggttgaga ccgtagatat
     1381 cgatatgggc catatactcc ttatttgagc tagagtactg gtcttcccat tccacatcaa
     1441 ggagacgttg ctcgtagttt tcttgggcac gcaggttcat acagtccaca cggtgaatag
     1501 ccacaccacg acccttggta atgtagccaa caatatcgtc accaggcacg gggttacaac
     1561 acttagcaat ccgcactagg agaccagaag caccttcaat aaccactccc ccctcatgct
     1621 tgaccttgag agtttcttta ttttcaacct tgacctcgcc acctttgaca agctcctctg
     1681 cctcagcctt ggccttggca cgctcttcct cacggcgttc cttttcagtc agacggttaa
     1741 agacggtaat cgcaccgatt tccccaaaac caatggccgc aaagagggag tcttctgtct
     1801 tgtaactggt cttttgcaga acttgatcca tgtggcgctt gtccataaat ttatttgcca
     1861 catagccatt ttcttggaac tgagccatca gcatctcacg acccttgttg acagacaatt
     1921 ccttatcttg gtttttaaag aactggcgaa tcttattgcg cgccttgcta gtcttgacca
     1981 tattgagcca gtcacggcta ggtccaaagg agttcgggtt ggcgataatt tcaacctgat
     2041 cccctgtctt taacttggtt gtcagtggaa ccatgcggcc attgaccttg gcaccagttg
     2101 ctttttcacc gaccttggta tggatttcgt aggcaaaatc aatcggtcct gaatctttgg
     2161 gaagagaacg gacagctcca tctggggtaa aaacgtaaat ctcctcagcc agatagtttt
     2221 ccttaacaga gtccacaaat tccttagcat catcagcctg gtcttggagc tccatcatct
     2281 ccttgatcca gttcattcca atagctgatt ccttgctgtt aacttgcccc tttatacctt
     2341 tcttataagc ccagtgagcc gcaaccccgt actcagccac ctcgtgcatt tccttggttc
     2401 gaatctggaa ttcaatcggc ccttttggtc cataaacagt cgtatggata gactgataac
     2461 cattagcctt gcggttggca atatagtctt tgaagcgacc tggcatcggt ttccaaaatt
     2521 catgcacgta accaagcatg gcataaacat cactttgggt atctaaaata caacgaatag
     2581 caatcagatc atagatttcc tcaaaccgtt ttctcttgtc ctgcattttg cggaaaattg
     2641 agtaaatatg cttgggacga ccataaatct tccctttcaa gtgacgttct gtcgtatact
     2701 cctctaattt tgtgactacc tcatccacca aggcctcacg ctccctgcgc ttttccttca
     2761 tcatatgggt aatcttgtaa aactccgttg gattgagata acggaaagac aagtcttcta
     2821 attcccattt gacactggaa atccccaaac gatgggcaag cggggcatag atttccatgg
     2881 tttctttgga aatacgctcc tgcttgtctt ttcgaagatg tttcagggtc cgcatattgt
     2941 gcaagcggtc agacagtttg accaaaataa cgcggatgtc ctcagacatg gccatgagca
     3001 tcttgcgatg attttccgct aattgctcct cgatcgattt gtactcgacc ttgccaagct
     3061 tggtaactcc gtcaacaatc atccgcacat caggaccaaa ctctctttcc aaatcgtcca
     3121 aagtcgcatc tgtatcttcc accacatcat gcaagaatcc acaagctact gttacagcat
     3181 ccagctttag cttagctaaa atacctgcca cttggatagg gtgaatgata taaggctcgc
     3241 ctgatttgcg atattgacca ctgtggcatt caacagcata gaccaaggcc ttatggacaa
     3301 aatgaacatc ctcttccgtt aaatattctt tggttaaagc gacaacttct tcgcctgtta
     3361 aattcacttc tttcggcatc tctactctcc aattcttcct accattttat cactttttta
     3421 agaatatgaa aactagattg gaacagaata agaaaaaaat aattcaaaat tgcttgataa
     3481 ttctgaatta ttggcccata atatactacg aagttagatt ttaaacttag gtgatagaag
     3541 gagagataga agaacggaaa ccatattgta acccaaagac tttctgattt ccccaattcc
     3601 attgaagata cgaaagataa acggtggaac tcgtatcaca tacactggta ccttgactgg
     3661 attttggaat taatactcaa tgaaaatcaa agagcaaact aggaaactag ccacaggttg
     3721 ctcaaagcac tgctttgagg ttgtagataa gactgacaaa gtcagtcaca tatatacggc
     3781 aaggcgacgt tgacgcggtt tgaagagatt tttgaagagt ataaaaatcc tcaagatact
     3841 ttcttctatc ctttagttta taaggagaat acctatgaaa aaactgctat ttctatcttt
     3901 gctctcctaa tgttaggagt ttgctgcctg ttcctattca gccagcaaag ctataaaaaa
     3961 ctcgttcaat actatgctaa cgaccagaac ctgcccagta ggataactta tagtgaatat
     4021 agcgacaaat gagaagccaa ctacggtagc actctaaaca tcacgtctat caaacaagct
     4081 aatgacggag tttatgcaac ctatgaaggg caattgacac ctttccaata ttgataaatt
     4141 gataaccagc ctgtcttcat ctagtcatgc tggtttttaa gttcatttta aatccttacc
     4201 tattctccct aactgtgcta tacttaattt atactcaatg aaaatcaaag agcaaactag
     4261 aaagctagcc gcaggctgtt caaagcactg ctttgaggtt gcagataaag ttgacgcggt
     4321 ttgaagagat tttcgaagag tattagtaca ttctttgaga ttggagttag tatgaaaatc
     4381 cataaaaccg tgaatcctgt tgcctatgaa aatacctatt atctagaagg cgaaaagcac
     4441 ctcatcgtcg tcgatcctgg tagtcattgg gaagccattc gtcagacaat cgagaagatc
     4501 aacaaaccga tctgtgctat tctcttgacc cacgcccatt atgaccatat catgagtctg
     4561 gacttggttc gcgagacgtt tggcaatcct cctgtctata tcgcagagag cgaagccagc
     4621 tggctctaca ctcctgtcga taatctctcc ggtctccctc gccacgatga tatggcagat
     4681 gtggtcacaa aacctgcaga acacaccttt gtctttcacg aagaatacca actagaggaa
     4741 tttcgtttta aggttctacc gaccccaggg cactctatcg gtggtgtttc cctagtcttt
     4801 cctgatgctc acctagtctt gacgggagat gctctattcc gcgaaactat cggacggacc
     4861 gaccttccga ctggtagcat ggagcaactc cttcatagta tccagaccca actcttcacc
     4921 ctaccaaact acgatgtcta tccaggacat ggtccagcta ctactatcgc tcacgaaaag
     4981 gccttcaatc cctttttcta gcaagatgat gacaatcgaa atttaagtaa actatccagc
     5041 aaatctttct attacaaaag gcatcctatc aaggttttca cacgtgattg gatgcctttt
     5101 ttctgatgac tagatttttt gtattaccaa ataatcacgc gctcctctgg tgaacgccac
     5161 attccgtctc cttctttgac atcataggtt gtaaagaaat cgtcgaagtt tggtacttgc
     5221 acattgacac ggagtttggc tggtgcgtgc acatcgacgc tagccaaaag tttcataaat
     5281 tctggtcgac ctttcatgcg ccagatgcga ccgaagttgt agaagaactc ttctgctgag
     5341 aagtctgctt ctctcttagc tgcttcaagc gctgctgcga ttcctcccaa gtcagccacg
     5401 ttttctgata cagtcaattt accgttaatg gttgctccat aagaatcctg tccatcaaat
     5461 tggtcaatga ctttttgtgt tttctccttg aaggcagcat agtcgctctc tgtccaccaa
     5521 tccttgaggc taccattttc gtcaaaggaa gccccgttag tatcaaaggc gtgggaaatt
     5581 tcatgggcaa tcactgcccc aataccaccg tagttagcag aagatgactg atgcaagtca
     5641 tagaaaggcg cctgtaaaat ggccgctgga aagacaatca ggttcttctg aggattgtag
     5701 taggcattga ccatatgagc aggcatgccc cattccttat agtctacagg ctggttccac
     5761 ttactccaac tgtgcttgat ttccacacgc gcaaaggcta gagcattctc aaaaagactg
     5821 gcagtttcat tcactacctt atccttgtaa cgtgcaggca attcttctgg atagccaata
     5881 taaggtttga tcacattgag cttcacgata gcctgtttac aggtttctgg agtgagccag
     5941 tcattcttaa gcagacgctc cttatagaca tcaatcatgg ttgccacttt tttctccaca
     6001 tccgccttgg cttctggaga gaacttctca cgggcgtacc aaagccccag ggcttgcttg
     6061 aaaggttcct gtgctagatg ataagctgct ttgaccttat cttttgcctc tggaactcca
     6121 gaaagggcac ggctgtaggc accagacaaa acacggatat cctctgttaa atagctggtt
     6181 gaaagattga caacactcaa aatcaaggtt gctttaagga gagaccaggc ttcctcactg
     6241 taaaattgct ctgctgcttg ccagaaacgt tcctcgtcta caataacctt gtctggtaat
     6301 tgcccaataa ctgctttgaa gaagtcatcc aaaggtaggg caggcgcgaa tttcttgaaa
     6361 tcttcgtaag aatatggatg atagagttta gcatattctg aactttcttc attagagagc
     6421 accactgccg caactcggcg gtccaattca agtctttttt ctagcaagtc ttcaatttct
     6481 tcatcagaga aatcataagc cttgaggaga tttgcgctgc tttctttcca aagagtcaag
     6541 agctcttcgc gctgaggatg ttcttctgca tagtaggtcg tatctggcaa gattgtgctt
     6601 ggagcgctag cccatagaac attgattcta gcatccataa agtctggcga tacaccaaaa
     6661 ggaaggaagt ttggttttcc tgcaagctca aactctgcta gtttagctgt aaaatccgca
     6721 aaagtttcca attcttggaa ttctttaagg agtggtaaga caggtgtgat accgtcagct
     6781 tctctcttgt caaaatcacg aactaggcgg tggtatttga caaagttttc caagatagca
     6841 tcctcaggca cttcttcacc tgctaaccac ttgtctgttg tcgccagcat caggtcttca
     6901 atttcctggt ctaaatcaac aaaacctcct gtttgagact tatctgctgg gatttcagct
     6961 gtctgttgcc attctccatt gatagcatca taaaaatcat cttgataacg tgtcatcttg
     7021 ttctcgcttt catttgtatt tgcatttatc ttaacaaaaa tcactaggga atgcaattaa
     7081 taaccattta actattattt tttagctatt ataaaaaacc tttccatgat aaaacgcata
     7141 attccaagtt ttttacactt gatactatgc gttttataat ttagaaattt atcctgaaag
     7201 ttatctttag aatctatttt
//
LOCUS       DAVBCB010000079         7162 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVBCB010000079 DAVBCB010000000
VERSION     DAVBCB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.079"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(741..1001)
                     /locus_tag="VQU28_001960"
     CDS             complement(741..1001)
                     /locus_tag="VQU28_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477252.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADIHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(1060..1611)
                     /locus_tag="VQU28_001961"
     CDS             complement(1060..1611)
                     /locus_tag="VQU28_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477253.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIYEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(1601..1798)
                     /locus_tag="VQU28_001962"
     CDS             complement(1601..1798)
                     /locus_tag="VQU28_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477254.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(1802..2386)
                     /locus_tag="VQU28_001963"
     CDS             complement(1802..2386)
                     /locus_tag="VQU28_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477255.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLPDGDIKQIKSVSNLLYGGAYALS
                     HSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(2794..3219)
                     /locus_tag="VQU28_001964"
     CDS             complement(2794..3219)
                     /locus_tag="VQU28_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477256.1"
                     /translation="MTSRVFIDADCISAFLWVGTEHLLEKLYSGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNYDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(3212..3706)
                     /locus_tag="VQU28_001965"
     CDS             complement(3212..3706)
                     /locus_tag="VQU28_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477257.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMVLGHYIKSTEQLLENNRISQGKYEELLLDAFRYDIVYGLDEEGE
                     LSFD"
     gene            complement(3752..3958)
                     /locus_tag="VQU28_001966"
     CDS             complement(3752..3958)
                     /locus_tag="VQU28_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477258.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(3945..4106)
                     /locus_tag="VQU28_001967"
     CDS             complement(3945..4106)
                     /locus_tag="VQU28_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2477259.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQU28_001968"
     CDS             complement(4471..5427)
                     /gene="lytA"
                     /locus_tag="VQU28_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HET2477260.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPDFTVEPEGLITVK"
     gene            complement(5803..7155)
                     /locus_tag="VQU28_001969"
     CDS             complement(5803..7155)
                     /locus_tag="VQU28_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HET2477261.1"
                     /translation="MDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVGRFLGQE
                     SLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWILGALLSI
                     LVMLLGFLGLYHLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLRSIGDSL
                     AALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRKSVPELL
                     PQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQTAARRI
                     MTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIFLFFASP
                     ALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPLVSSFIE
                     LIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAILATKVQS
                     "
BASE COUNT         2298 a         1486 c         1132 g         2246 t
ORIGIN      
        1 tggtttcgca ggaagtctat tatatatgca cagacatatg aaatcttttt gagacagcag
       61 ttatgatata tttatccatt cacaacatct tctgtactaa tactacaata ttggtacatt
      121 ttgattacac aatcgtttca aaaaatatga tggaagcctg agtattttat agtaattccc
      181 ttcctatcaa ctagatcaac cagttgagtg ttgaatgttg aaacacccta gcctcagcaa
      241 aggactatga tcagcatttc ctagccaata ggacgctcca aatcacctat tctaagtcca
      301 tcactagctt ggtcagtaag atacagactg caaggcgaga ctgacgtggt tgatgagatt
      361 ttggaagagt attacaatca tagattcacc agtagacttg accgcagatt taactatagt
      421 agattgaaac tagaatagta catctctgct tctaaaacat tgttagaaat cgatttgact
      481 gtcctgatca atttgtcctg ttctcgtttc atttcactat actaaaaaac cacctgaaac
      541 aaatcaagtg actagaggat aaaaacaaag actaggcaaa aagttttgaa aagcaaaaaa
      601 cgcataatat caggtcttga aaaaccttga tactatacgt ttgattgtgg gaagattgac
      661 ttcattttct cctgaaattg aagttttttc tagtttccag atatagacga attcaatata
      721 gccatctcgc tacaattatt ttaaccatcg agaaaatttt tcagaatttc aaaaatttca
      781 ttttgatcca atgaaatgtc atcattttga agaatagttg acttcacagg ctctaattct
      841 atcacaaagc ggtgtatgtc tgcttttacc gcactacttg atgttatagc cgataacttt
      901 tcatctccta gcaaggaagc tgtcaaacgg gtaaggtcat tcaaatgctt tttaatggac
      961 ttacttaaac cttgagcacc tgtagcagag cgttccctca tatccagcca agctttagcc
     1021 ttaaaaataa ttaatactca atgaaaatca aagagcaaac taggaagcta gctgcaggct
     1081 gctcaaaaca ctgttttgag gttgtagatg gaagctgacg tggtttgaag agattttcga
     1141 agagtataaa ccacaattac tcaataccga atacccctga atggtttctt tttcatgcac
     1201 caatatatta taataatctt catccaataa taaggctgat aaactagcat cttggtcaaa
     1261 atgtaaggga atttctcgac cgtccttctt taatggatat tctggtaaga tactaaatag
     1321 ttcaatcata gaaggaaact caggattagt tgttgtaaat cgaaaaagct gcgctttctc
     1381 atctttctga cttccttgat actctcccaa ttctaaaaaa tgattcaagg tagtataaaa
     1441 ttccttattt tttacttcat aaatgatgac catatcataa tcttttgttg tgcgactttt
     1501 aaatccttgc gaatccaata cgatagaggt agcagttccc ccaatcagaa cataatagtt
     1561 ctgaaaatcc gcaaacattt cttgaaaaat aactttcgta ttagctggca tcatcttctc
     1621 ccagatactg taatatcata ttttctagtg cttcactctc ttcctctata cgtgggtcat
     1681 catcatcttt taaggtcaaa taaagagaaa tcggatctac aaattgttta tcatgattat
     1741 ttttaaaatc attccaaaac tcagatacaa aaggacgata tttccatatc tctagcatct
     1801 ttcattttaa aacatgctga gaaagtggca aggataactg attgaatttt ctctgccata
     1861 tgacatagct agtattttca tccgtttcag ctaaaaaagt tgaatgcgac aaagcataag
     1921 caccaccata tagaaggtta gaaacagatt ttatctgctt tatatcgcca tctggcaata
     1981 aaaaggcaat ccacgttaat tgttcgctag gtgttaattc cttagggatt tcagtatcat
     2041 tcgcattgag tactagtccc aatggaggga agaagaggtt tcccttaaag tctacaaacg
     2101 gaactctagc ttgaagtaat tgcttttttt cactgtctga taacttcgaa aacaccaaaa
     2161 caacatccat attggctttt tcacccatag tgcgagcctg agtaacaaat gaactcaaac
     2221 tcccccttct cttttcttta atcaataaaa atgattcctt atgaaaagtc atttgagtat
     2281 actcaaacct ttttacaaaa ctgattggta aagtcaaagt cagttcctca atactgacat
     2341 caacataaaa ggattgaaaa attgacatta tctttttgac attcatgttt tttccttatt
     2401 ctatcatttt tattatattt tatcattcgg tataataaaa tataagtttt ttgataaaat
     2461 aaccaaaaac tactcgctat cccctctcac attaccctac atatcgtttg acatgcgact
     2521 gatagttcag gaaaacttca aggagaactt ttctctcatc cactatgcag gacttactat
     2581 ttcacttcta ctcctatagg ctcaatttga cacttttctt tttcgaattg ctcatatgcc
     2641 tcctgaatag tgagttcgat ttatccaaat ttatttttgt ctattttgat gaatacttcc
     2701 acgaagatag tctgaaaatg aatttgcacc aattttcctt ctctttttaa gcatattatt
     2761 ccagatatga ttgccctctt gttcagtaat aaattacgct ttaaacgctt caatcagtat
     2821 atctcctgtt gtcatatgtt ctaaagaaaa ttcttctaca tatggtttaa catctcttag
     2881 gttattactt cctaatatcc cattatgctt tttcgctaag aaaatagaag tcgcttctcc
     2941 cttaccaata atcttgttac tatcataatt tcttgttaaa tctctatata atgcgtattc
     3001 ttcagttcca atgtctatgc tcacaatctc agctgaaccc ttagttacca actggtctat
     3061 cctagatttt aaatggggaa ttgtaggtat attgatttca tcatacacct cttgtggaat
     3121 aacaatttta cccgaataga gcttttctaa aagatgttca gtgccaaccc ataaaaatgc
     3181 tgaaatacaa tctgcatcaa taaatacacg actagtcaaa cgacaactcc ccctcttcat
     3241 ctagcccata tacaatatca tatctgaaag catctagtaa cagttcctca tacttccctt
     3301 gcgaaattct gttattttct aaaagttgtt cagtcgattt aatatagtgc cctaatacca
     3361 tttccttttt actttctgac aaaggacgat ataaacttgt atcatagcct aatcttgaag
     3421 ctgtctctat aacactaata tccatatttt taatttcttc tgcatcaagg tatccatcat
     3481 ttctcaatct atataacata gctttatgac tgataccata aaactgaccc aattttataa
     3541 tatcttctac ttcaagatga gttctattgg cattttctct gatttcctca accatcctat
     3601 acagtgaaga tgggaaaatt aaaaaataag aagcaaactg atccgctttt ctttcagttt
     3661 catctccttc accaatcaag ataagactga ctgaactctt cttcacctca tcataataaa
     3721 gatgatacag tccatgtgct aaagaaaatc tttaccttcc taatggcatg tctgaattga
     3781 ctgcaatgag actgaactga gttcctttat aacagactcc gctaatattc tttccgagtc
     3841 cataaaatac cagcgtcaaa ttttctatct tttgtaccaa tttaaaaata tctatcggcg
     3901 attcaccatc agctcccaac ttttttctaa gatttgaagc tttattactt aaatccattc
     3961 gtgatattcc tttcaccttt ttccattttg gcaatcatgc tttgattcaa agacaaatac
     4021 tcagcaatct tatcttgagt aagatttgaa gatattctaa gttcttttat tctttttcca
     4081 acatcacata catttatcat attcatacac ctctcataaa aagaatagca cattcttgtc
     4141 ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg atgctactta
     4201 aaaatctaaa attgatgata ttaaacctat ttgatgagtt cctatcaaaa atcgtatctt
     4261 caacctcaaa acagtactta aagctatccg actcggttta cattgtcaaa tttagatttt
     4321 atttgagcat aactttctag tttgcttttt gatttttgtt taatatagta acaaaaattg
     4381 aaggaaatat ctccacaaga aaacgcatac tattaagctt tttcaagacc taataatatg
     4441 cgctgttctg atttgaaaga cattccatta ttattttact gtaatcaagc cttcaggctc
     4501 tactgtgaag tctggcttgt ctgccagtgt tccgtctggt ttgaggtagt accagcctgt
     4561 tccgtccgct gactggataa aggcatttga taccatggcg ccttctttag cgtctaagta
     4621 gtaccaagtg tccttgtact tgacccagcc tgtcttcatg gcaccttctt cgttgaaata
     4681 gtaccactta tcagcgattt tcttccagcc tgtagccatt tcgcctgagt tgtcgaacca
     4741 gtaccagttg ccgtctgtgt gcttcctcca gcggtctgca agcatatagc ctgaactgtc
     4801 aaagtagtac caagtgccat tgattttctc aaacttgtct tttggataag agccgtctga
     4861 atgtacgtac cagtagccag tgtcattctt ctgccagcct gtttcaatcg tcaagccgtt
     4921 ctcaatatca tgcttaaact gctcacggct aatgccccat ttagcaagat atggataagg
     4981 gtcaacgtgg tctgagtggt tgtttggttg gttattcgtg caatactcgt gcgttttaat
     5041 tccagctaaa ctccctgtat caagcgtttt cggcaaacct gcttcatctg ctagattgcg
     5101 taagagttcg atataaaggc ggtagtccgt catgaactct tctttggttg aatggctttc
     5161 aatcagttca accgctgcat aggtctcagc attccaaccg cccccaacgt cccaggcacc
     5221 attatcaaca ggtcctacct gcatgatgca accgttccca acaatgtgcg agaaaaaacc
     5281 taattctggg tctttccgcc agtgataatc cgcttcattc tgtacggttg aatgcggatt
     5341 cccagttgag tgtgcgtgta cttgcctata tggttgcacg ccgacttgag gcaaatctgt
     5401 tcttaattta ctcacattaa tttccatatt ctactcctta tcaattaaaa caactcattt
     5461 tttacaatcc agaaccagaa actcctttat ttctacctta caaagaagac aatcttagtc
     5521 acgattaagc ttgtagatag aacctcaaaa cgcactattt tgacactgta aataggactg
     5581 acaaggtctg cattctatct acaataacac cccagactaa aaagcttttc aaaagtatat
     5641 ttttacagtc tctatatgtc cttttcataa atactatact ttattatatc acataaaaga
     5701 agtcaaaagt ctgttaaact attttcaaca ccaaactaaa gaagagaaca caagtttttt
     5761 cgatgttcac tagaggaaat ggattttatt cagtaaatcc aactaggatt gcactttggt
     5821 tgccaaaatt gcctttcctt cttttatcaa gggatgacgg aatagtgaga agtacagttg
     5881 aactgtcatg gcaacccaga taagaggttc acaaaggata acacccttat atcctgccca
     5941 aggaataatc aaaaccacaa aaacgatttt tccgattagt tcaataaagc tagaaactag
     6001 aggaaggatc ttctgcccca agccctgcaa gcaattgcga taaatcaaca agaggctcaa
     6061 aatgggataa aaggctgaac tgatttgcag atagagactt ccattttcta tcaagtaacc
     6121 atctgtcgaa ctagccaaga aggaaaccaa ggctggactg gcaaaaaaga ggaaaataca
     6181 aacaaaaact gcccaggata tacttaaacg actgccgatt cgaagacctt gaacaatgcg
     6241 gtcaggtcgc ttagctccta gattctgaga agcaaaggtc gtcattgatg cagaaatagc
     6301 ggtcatagga agaagggcaa aggtcataat gcgtcgagct gccgtctggg cactaataat
     6361 cactgcacca aatgtattaa cagaaaactg taaaatcaca ctgccgatag atacaattga
     6421 actcatcaag cccatagcca aaccttgctc caagagatcc gcgtacaagc ttttgtccca
     6481 tttgaaatgt ttaaactgtg gcaagagttc tggcacactt ttacgaatat aataaaagca
     6541 gagaaccgct gataaacctt gcgaaataat ggtagcaagt cctgcggatt gaactcccag
     6601 atgcaattgc gtaataaaat agagatccag aaccacatta accaaggcag agaaaatcag
     6661 aaatcccagg gctgctagac tgtcaccaat agaccgcaac aagcctgcaa aaagattata
     6721 agcaaagctg acacctacac aggtcacaat catagaaata tattgataag attgaggaag
     6781 aatttctgca ggagtatcta agtattgcaa gagatgatac aagccaagaa agcccagcaa
     6841 cataactaga atgctcaaaa gagcacctaa aatccaagta gctgctactg cttccttgat
     6901 tttagtgaaa ttccgagccc cataataacg agcaatgaca atccccatgc cattgccaac
     6961 accaagtgta aaacctacaa tcaggtcaaa aatcgctgtc gtcgctccta ctgcagccaa
     7021 ggattcttga ccaagaaatc gtccaacaat caagacatca gcagtgttat agagctgttg
     7081 aaaaatattt gatagcaaaa ttggaaaggt gaagcttaag agcgagggaa gaatcggacc
     7141 atgtatcagg tccactgttc gt
//
LOCUS       DAVBCB010000080         7014 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVBCB010000080 DAVBCB010000000
VERSION     DAVBCB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.080"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(317..2008)
                     /locus_tag="VQU28_001970"
     CDS             complement(317..2008)
                     /locus_tag="VQU28_001970"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HET2477262.1"
                     /translation="MNTKELIASELASIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            2144..2590
                     /gene="argR"
                     /locus_tag="VQU28_001971"
     CDS             2144..2590
                     /gene="argR"
                     /locus_tag="VQU28_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HET2477263.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            2641..5175
                     /gene="mutS"
                     /locus_tag="VQU28_001972"
     CDS             2641..5175
                     /gene="mutS"
                     /locus_tag="VQU28_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HET2477264.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKLDSQNNFLVAIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSCQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPALAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLITGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTENPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            5322..>7014
                     /locus_tag="VQU28_001973"
     CDS             5322..>7014
                     /locus_tag="VQU28_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HET2477265.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLGALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELLGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDVPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQEGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         1901 a         1370 c         1667 g         2076 t
ORIGIN      
        1 tttggttagg tgaattagtg aagcgtttag gcaagtgtct ctggttacga cgtcatggac
       61 tctaaatcga ttatatttag gggtcatgac tagtgaagca gttagctagt tcgcatataa
      121 gcggctagcg tctaacaatt aggaacttta gttccaataa ctttaagatt acgacgtttt
      181 aggacataaa tcgatcatat ttatgtccta aaactagtga agtatgtcct aaaactagtg
      241 aagcgcctag ccaaagtccg aataggattt ggcgttagtt acttagattg ctttgcaatc
      301 aagtaacttt ggcgatttac atcttctctg gcgcttctac tccaagcaag cgaagggctt
      361 ctttgagaac gactgcggtt gcgtagctga gggctagacg gctgtcgcgt tctgggcttt
      421 catccaagat acgtgtatgt gcatagtatt tgttaaagga ttgagccagg ctaattgcaa
      481 atttagcaat gatagaaggt tcaaagttat ctgccgcacg gttgataata cgtgggaagt
      541 cttgaatgag tttaatgatt tcccagcttt cagtatcatt caagctatag ttgccagctg
      601 tttctggttt gaaatcggct ttgcgtaaga tagattggat acgagcgtag gcatattgaa
      661 cgtaaggtcc agtttcaccc tcgaaggata ccatagcctc taggtcgaag tcgtatccat
      721 ttgtacggtc ggttttgagg tcatagaatt taatggctcc aaccccaaca gcatgtgcta
      781 cttggtcttt gttttctagt tcaggatttt tagcctcgat ttggaccttg gcacggctaa
      841 cagcctctgc aacagtaggc tctagcaaga tgacattccc tttacgagta gagagtttct
      901 tcccttcttt tgtaaccaaa ccaaaaggaa cgtgagtaat gtcgtcactc cagtcgtagc
      961 ccatctcttg caagacagct ttgagctgtt taaagtgggc agattgttct tgaccaacga
     1021 catagataga tttagcaaat tggtattcgt ttttacggta gagggctgca gccaagtcac
     1081 gtgtgatata gagagttgca ccatcagact tcttgatgag ggctggatgt tcaattccgt
     1141 atttctcaag attcacaact tgggcacctt ctgattcaag aagtagtcct ttttcagaaa
     1201 gaatgtctac aactgcatcc atcttatcat tgtagaaggc ttctccgtta tagctgtcaa
     1261 attcaacctt caattcattg taaaggcggt taaattccac taaactttca tcgcggaacc
     1321 attgccaaag agcgagagct tcctcatctc cattttcaag tttacggaac cattcgcgcg
     1381 cttcttcatc caagctaggg tcattttcag cttcagcgtt gatgcggaca tagagtttaa
     1441 ggagttcatc gattggatga gcttttacag cttcttcgtc gccccatttt ttgtaggcaa
     1501 caatcaacat cccaaattgt ttaccccagt ctcccaaatg gttgaccttg accgtttgat
     1561 aaccgatttt ttggaaaata tgtgacaagc tatctccgat aacagttgaa cgcaggtggc
     1621 caatagaaaa tggtttagcg atattcggac tagacatgtc gataacaaca ttttcttgtt
     1681 taccaatatt ttggtcagca tagtgttctt tttcagtggt aacagcttgc aatacttgag
     1741 cagaaatggc agatttatca aggaaaaagt taacgtaagg tcctgttgcg acaacttttt
     1801 caaaggcttg gctgttcatt ttttcagcca gttcagccgc aatcatttgt ggtgctttac
     1861 gttcgacttt ggcaagagaa aaagcaggga aagcgatgtc acccatttct gagtttttag
     1921 gggtttccag taactttaaa atagcctctt ggtccaggct atcaatgatg ctagccaatt
     1981 cgctagcaat caattctttt gtattcatta agagctcctt tttggacttt tctactattt
     2041 tatcacaatt ttaaagaaag aagaaaaaat ttttgaaatc tcctgttttt ttggtataat
     2101 atggttataa atatagttat aaatatgcac gcaagaggat tttatgagaa aaagagatcg
     2161 tcatcagtta ataaaaaaaa tgattactga ggagaaatta agtacacaaa aagaaattca
     2221 agatcggttg gaggcgcaca atgtttgtgt gacgcagaca accttgtctc gtgatttgcg
     2281 cgaaatcggc ttgaccaagg tcaagaaaaa tgatatggtg tattatgtac tagtaaatga
     2341 gacagaaaag attgatttgg tggaattttt gtctcatcat ttagaaggtg ttgcaagagc
     2401 agagtttacc ttggtgcttc ataccaaatt gggagaagcc tctgttttgg caaatattgt
     2461 agatgtaaat aaggatgaat ggattttagg aacagttgct ggtgccaata ccttattggt
     2521 tatttgtcga gatcagcacg ttgccaaact catggaagat cgtttgctag atttgatgaa
     2581 agataagtaa ggtcttggga gttgctctca agacttattt ttgaaaagga gagacagaaa
     2641 atggcgatag aaaagctatc acccggcatg caacagtatg tggatattaa aaagcaatat
     2701 ccagatgctt ttttgctctt tcggatgggt gatttttatg aattatttta tgaggatgcg
     2761 gtcaatgctg cgcagattct ggaaatttcc ttaacgagtc gcaacaagaa tgccgacaat
     2821 ccgatcccta tggcgggtgt tccctatcat tctgcccaac agtatatcga tgtcttgatt
     2881 gagcagggtt ataaggtggc tatcgcagag cagatggaag atcctaaaca agcagttggg
     2941 gttgttaaac gagaggttgt tcaggtcatt acgccaggga cagtggtcga tagcagtaag
     3001 ctggacagtc agaataattt tttggttgcc atagaccgcg aaggcaatca atttggccta
     3061 gcttatatgg atttggtgac gggtgacttt tatgtgacag gtcttttgga tttcacgctg
     3121 gtttgtgggg aaatccgtaa cctcaaggct cgagaagtgg tgttgggtta tgacttgtct
     3181 gaggaagaag aacaaatcct cagctgccag atgaatctgg tactctctta tgaaaaagaa
     3241 agctttgaag accttcattt attggatttg cgattggcaa cggtggagca aacggcatct
     3301 agtaagctgc tccagtatgt tcatcggact cagatgaggg aattgaacca cctcaaacct
     3361 gttatccgct acgaaattaa ggatttcttg cagatggatt atgcgaccaa ggctagtctg
     3421 gatttggttg agaatgctcg ctcaggtaag aaacaaggca gtcttttctg gcttttggat
     3481 gaaaccaaaa cggctatggg gatgcgtctc ttgcgttctt ggattcatcg ccccttgatt
     3541 gataaggaac gaatcgtcca acgtcaagaa gtagtgcagg tctttctcga ccatttcttt
     3601 gagcgtagtg acttgacaga cagtctcaag ggtgtttatg acattgagcg cttggctagt
     3661 cgtgtttctt ttggcaaaac caatccaaag gatctcttgc agttggcgac taccttgtct
     3721 agtgtgccac ggattcgtgc gattttagaa gggatggagc aacctgctct agcctatctc
     3781 atcgcacaac tggatgcaat ccctgagttg gagagtttga ttagcgcagc gattgctcct
     3841 gaagctcctc atgtgattac agatggggga attatccgga ctggatttga tgagacttta
     3901 gacaagtatc gttgcgttct cagagaaggg actagctgga ttgctgagat tgaggctaag
     3961 gagcgagaaa actctggtat cagcacgctc aagattgact acaataaaaa ggatggctac
     4021 tattttcatg tgaccaattc gcaactggga aatgtgccag cccacttttt ccgcaaggcg
     4081 acgctgaaaa actcagaacg ctttggaacc gaagaattag cccgtatcga gggagatatg
     4141 cttgaggcgc gtgagaagtc agccaacctc gaatacgaaa tatttatgcg cattcgtgaa
     4201 gaggtcggca agtacatcca gcgtttacaa gctttagccc aaggaattgc gacggttgat
     4261 gtcttacaga gtctggcggt tgtggctgaa acccagcatt tgattcgacc tgagtttggt
     4321 gacgattcac aaattgatat ccggaaaggg cgccatgctg tcgttgaaaa ggttatgggg
     4381 gctcagacct atattccaaa tacgattcag atggcagaag ataccagtat tcaactgatt
     4441 acagggccaa acatgagtgg gaagtctacc tatatgcgtc agttagccat gacggcggtt
     4501 atggcccagc tgggttccta tgttcctgct gaaagcgccc atttaccgat ttttgatgcg
     4561 atttttaccc gtatcggagc agcagatgac ttggtttcgg gtcagtcaac ctttatggtg
     4621 gagatgatgg aggccaataa tgccatttcg catgcgacca agaactctct cattctcttt
     4681 gatgaattgg gacgtggaac tgcaacttat gacgggatgg ctcttgctca gtccatcatc
     4741 gaatatatcc atgagcacat cggagctaag accctctttg cgacccacta ccatgagttg
     4801 actagtctgg agtctagttt acaacacttg gtcaatgtcc acgtggcaac tttggagcag
     4861 gatgggcagg tcaccttcct tcacaagatt gaaccgggac cagctgataa atcctacggt
     4921 atccatgttg ccaagattgc tggcttgcca gcagaccttt tagcaagggc ggataagatt
     4981 ttgactcagc tagagaatca aggaacagag aatcctcctc ccatgagaca aactagtgct
     5041 gtcactgaac agatttcact ctttgatagg gcagaagagc atcctatcct agcagaatta
     5101 gctaaactgg atgtgtataa tatgacacct atgcaggtta tgaatgtctt agtagagtta
     5161 aaacagaaac tataaaacca agactcacta gttaatctag ctgtagcaag gagacttctt
     5221 tgacaattct ccactttttt gctagaatga catcacacaa acagaatgaa aaggagctga
     5281 cgcattgtcg ctcccttttg tctatttttt aaggagaaag tatgctgatt cagaaaataa
     5341 aaacctacaa gtggcaggcc ctggcttcgc tcctgatgac aggcttgatg gttgctagtt
     5401 cacttctgca accgcgttat ctgcaggaag tcttaggcgc cctccttact gggaaatatg
     5461 aagctattta tagtatcggg gcttggttga ttggtgtggc cgtagtcggt ctagttgctg
     5521 gtggactcaa tgttgtcctc gcagcctata ttgcccaagg agtttcatcc gaccttcggg
     5581 aggatgcctt ccgtaaaatt caaacctttt cttatgctga tattgaacaa tttaatgcgg
     5641 gaaatctagt cgttcgaatg acaaatgata tcaaccagat tcagaacgtt gtcatgatga
     5701 ccttccagat tcttttcaga cttcccctct tgttcatcgg ttcgtttatc ctagcggttc
     5761 aaaccttacc ttctctgtgg tgggtgattg ttctcatggt agtcttgatt tttggtttga
     5821 ctgctgtcat gatgggaatg atggggcctc gttttgccaa gtttcaaacc cttcttgagc
     5881 gcatcaatgc cattgccaag gaaaatttac gtggcgttcg tgtggtcaag tcctttgtcc
     5941 aagaaaaaga gcaatttgct aagtttacag aggtctcaga cgagcttctt ggtcaaaacc
     6001 tttacattgg ttatgccttt tcagtagtgg aaccctttat gatgttggtt ggttacgggg
     6061 cggtcttcct ctctatttgg ctggtcgcgg gaatggttca gtcggatccg tctgttgttg
     6121 gttccatcgc ttcttttgtt aattacctaa gccagattat ctttaccatt gttatggttg
     6181 gatttttggg aaattctgtc agccgtgcca tgatttccat gcgtcgtatt cgagaaattc
     6241 ttgacgcaga gccagctatg accttcaagg atgtcccaga tgaagagttg gttggaagcc
     6301 ttagctttga aaatgtgacc tttacctatc caatggacaa ggaaccgatg ctgaaagatg
     6361 tgagctttac tattgaacct ggtcaaatgg ttggtgtagt tggagcgact ggtgcaggaa
     6421 agtcaacctt ggctcaattg attccacgtc tctttgatcc acaggaaggg gctatcaaaa
     6481 tcggaggtaa ggatattcga gaagtgagtg aaggaaccct gcgtaaaaca gtttccatcg
     6541 ttctccaacg tgccattctt tttagtggaa cgattgcaga taacttgaga caggggaagg
     6601 gaaatgctac tctatttgaa atggagcgcg cagccaatat tgcccaggct agtgaattca
     6661 ttcatcgtat ggagaaaacc tttgaaagtc cagttgaaga acggggaacc aatttctctg
     6721 gtggacaaaa acaaaggatg tcgattgcgc gtgggattgt cagcaatcca cgtattctga
     6781 tttttgatga ttcgacctca gccttggatg ccaaatcaga gcgcttggtg caagaagctt
     6841 tgaataagga cttgaagggg acgacaacca ttattattgc tcaaaaaatt agctcggttg
     6901 tccatgcaga caagatcttg gttctaaatc aaggacgatt gattggtcaa ggcacgcatg
     6961 cagacttggt tgccaacaat gccgtttacc gtgaaatcta tgaaacacag aaat
//
LOCUS       DAVBCB010000081         6917 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVBCB010000081 DAVBCB010000000
VERSION     DAVBCB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6917
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.081"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..344
                     /locus_tag="VQU28_001974"
     CDS             <1..344
                     /locus_tag="VQU28_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2477266.1"
                     /translation="SLVAGLTNGELIAPMTYEETMTSDFFEVWFQKFFLPTLTTPSVI
                     IMDNVRFHRMGKLELLCEEFGHKLLPLPPYSPEYNPIEKTWVHIKKHLKKVLPSCNTF
                     YEALLSCSCFN"
     gene            405..1367
                     /locus_tag="VQU28_001975"
     CDS             405..1367
                     /locus_tag="VQU28_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HET2477267.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSSNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVIIFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPVEPLRYE"
     gene            1544..2551
                     /gene="gap"
                     /locus_tag="VQU28_001976"
     CDS             1544..2551
                     /gene="gap"
                     /locus_tag="VQU28_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HET2477268.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2618..3454)
                     /locus_tag="VQU28_001977"
     CDS             complement(2618..3454)
                     /locus_tag="VQU28_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HET2477269.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQYLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETYVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3580..5775
                     /locus_tag="VQU28_001978"
     CDS             3580..5775
                     /locus_tag="VQU28_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HET2477270.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMASQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVAAVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIAYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5828..5980
                     /gene="rpmG"
                     /locus_tag="VQU28_001979"
     CDS             5828..5980
                     /gene="rpmG"
                     /locus_tag="VQU28_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HET2477271.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5990..6166
                     /gene="secE"
                     /locus_tag="VQU28_001980"
     CDS             5990..6166
                     /gene="secE"
                     /locus_tag="VQU28_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HET2477272.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6222..6758
                     /gene="nusG"
                     /locus_tag="VQU28_001981"
     CDS             6222..6758
                     /gene="nusG"
                     /locus_tag="VQU28_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HET2477273.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2149 a         1202 c         1502 g         2064 t
ORIGIN      
        1 tttctttggt tgcaggtcta acaaatggtg aattaatcgc tccaatgact tacgaagaga
       61 cgatgacgag cgactttttt gaagtatggt ttcagaagtt tttcttacca acattaacca
      121 caccatcggt tattattatg gataatgtaa gattccatag aatggggaag ctagaactct
      181 tgtgtgaaga gtttgggcat aaacttttac ctcttcctcc ctactcacct gagtacaatc
      241 ctattgagaa aacatgggtt catatcaaaa agcacctcaa aaaggtatta ccaagttgca
      301 atacctttta cgaggctctt ttgtcttgtt cttgtttcaa ttgactatat atcacggata
      361 cagttgggaa agaagctatt aaacatttgg agagtccttt tgggatgaac atgtcaaata
      421 ttactataga tgggcaagaa atggatctga gtcaaacgat gccttcttat gctcaagtag
      481 tgagtcttta tgatgataca agtatctctg ttagtagtaa tgagacggac aaagtgttgg
      541 ctggctccct ctatactgat acaaatgaac agggattaac gattccaagc agtttactaa
      601 aaaattggaa tgaacagaca ggaaaaaatt tgacagctaa tgatcttatt ggcaaatcag
      661 tctcagccag cattgtagaa agtgctgccg aaactagtaa gattgctcaa tttcaaacga
      721 agattgtacg tgtaatcaat gatgaagatg acatggagga cagcaacagt ttcatgctgt
      781 ctcatcaaat ggaaacgatt ttgaaagagg ctggatttac gaaagctgta tcttatttta
      841 tcttggaact caaagatcca tcacagacaa aagtagtaac agaagaatta cagaaaaata
      901 agaagtatac tgtgctttct caacagagag ttcttgatat tgtgattatc tttattcgtg
      961 ttattcaggg attattgatt gtgctttcat cacaagctat tgtggtagca gcggttatga
     1021 ttggtatcat tatttacatc aatatcatgc agcgttccaa ggaaataggt gtcatgaagg
     1081 cagttggtta tcagaatcgt gatgtcaaag gaatttttat ttacgaggct atctggattg
     1141 taggcatcgc cttgctgctg gcatttttgg ttgcacaagg ggtgggaagt ttggcgaatg
     1201 cgattgtaag tcacttttac ccatccatca ctaaagtttt tgaattaaat cttttatctg
     1261 ttttaggaac tctagttttc gctctattac ttggttatgt ctcagcctac ttcccggcgc
     1321 gtaagattag taaaatggat cctgtagaac cgttacgcta tgaatagtaa gggattcctt
     1381 gatttacagt cttaaaaaga aactaacata gaaaaacaat ggacatactt gcaattcaac
     1441 tcaaatagtt gtataatagg tttgttgaaa ggtgatttgt agtgattcaa gttactcttt
     1501 tcacaataaa aatttcatga ttttcataag gaggaaatca ctaatggtag ttaaagttgg
     1561 tattaacggt ttcggacgta tcggtcgtct tgctttccgt cgtatccaaa acgtagaagg
     1621 tgttgaagtt acacgcatca acgaccttac agatccagtt atgcttgcac acttgttgaa
     1681 atacgacaca actcaaggtc gtttcgacgg tactgttgaa gttaaagaag gtggatttga
     1741 agttaacggt aaattcatca aagtttctgc tgaacgtgat ccagaacaaa tcgactgggc
     1801 tactgacggt gtagaaatcg ttcttgaagc tactggtttc tttgctaaga aagaagcagc
     1861 tgaaaaacac cttaaaggtg gagctaaaaa agttgttatc actgctcctg gtggaaacga
     1921 cgttaaaaca gttgtattca atactaacca cgacgttctt gacggtactg aaacagttat
     1981 ctcaggtgct tcatgtacta caaactgctt ggctccaatg gctaaagctc ttcaagacaa
     2041 ctttggtgtt gttgaaggat tgatgactac tatccacgct tacactggtg accaaatgat
     2101 ccttgacgga ccacaccgtg gtggtgacct tcgccgtgct cgcgctggtg ctgcaaacat
     2161 cgttcctaac tcaactggtg ctgcaaaagc tatcggtctt gtaatcccag aattgaatgg
     2221 taaacttgat ggatctgcac aacgcgttcc aactccaact ggatcagtta ctgaattggt
     2281 agcagttctt gaaaagaacg ttactgttga tgaagtgaac gcagctatga aagcagcttc
     2341 aaacgaatca tacggttaca cagaagatcc aatcgtatct tcagatatcg taggtatgtc
     2401 ttacggttca ttgtttgacg caactcaaac taaagttctt gacgttgacg gtaaacaatt
     2461 ggttaaagtt gtatcatggt acgacaacga aatgtcatac actgcacaac ttgttcgtac
     2521 tcttgaatac ttcgcaaaaa ttgctaaata attcttgagt tgatagaaag caaggctttg
     2581 tggtcttgct ttttatatgg aaaaatggat gacacgatca tccattcttt tttaattctt
     2641 tttcaaatgt atttgaaagg gtagtgaaag ttagcttctc taaagtaagt gggtgggtaa
     2701 aggaaagtcg gaaggcatga agcataagcc gacttgtctt tgatttacta ttatagagag
     2761 ggtcgcccag gataggaaga ttatgatgcg aaaggtgcac acgaatctga tgggttcgcc
     2821 ctgtctttag cttgcaacga gccaaggaag tcttgtttga gaattgcttt aatctgctta
     2881 catacgtttc agcatattgc ccattttttg catcaactat tctttttcta cgatcatggc
     2941 gatcacgtcc aattttgtct ctgaaaacaa gttcttttct gttgatattt ccatcaacta
     3001 gagcccaata ttctctagaa atctcttttt tctccaataa gcgattgaga atgggcagga
     3061 taaaaggatt tttggcaaag agaactaagc cactggtttc catgtccaga cgatgaacga
     3121 catagcaggt ttggccaaca taggtactga catggttaag aagggcaatt tcgtttggtt
     3181 gattaccatg cgttttcatc ccctctggtt tgtttacaat aatcaagtat tgatcttgat
     3241 aaacttcctg cactaagtct gggttgcccc aagggatcgt cttttgggga taatcttcct
     3301 cgtcaaaagt caactggcaa acatctccag gatttacgat ttcgttccag tggacttctt
     3361 cttgatttat caaaatatgt ttcttgattc tcaaaaaatg acggattttt ctagggatga
     3421 ggagttgttc ctcaagtaat tgctttaccg tcatttgagg tagagaggcg ggtaatgtaa
     3481 atgtgaattg catacagata ttgtaacaaa aaaagcccta tttggatagg aaatagctaa
     3541 attcttgtct tcctatgatg aagatgataa aataaacgca tgaaattaga taaattattt
     3601 gagaaatttc tttctctttt taaaaaagaa acaagtgaac tagaggactc tgattctact
     3661 atcttacgtc gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc gattcgaaaa
     3721 ttctggcgtc gttatcatct aacaaagatt atccttatac taggtttgag tgcaggcttg
     3781 ctagttggaa tctatttgtt tgctgtagcc aagtcgacca atgtcaatga tttgcaaaat
     3841 gccttgaaaa ctcggactct tatttttgac cgtgaagaaa aagaggctgg tgccttgtct
     3901 ggtcaaaagg gaacctatgt tgagctgact gatatcagta aaaacttgca gaatgctgtt
     3961 attgcgacag aagaccgttc tttctataaa aatgacggga ttaactatgg ccgtttcttc
     4021 ttggctattg tcactgctgg acgttcaggt ggtggctcta ccattaccca acagctggct
     4081 aaaaacgcct atttatcaca ggatcaaact gttgagagaa aagcgaaaga atttttcctt
     4141 gccttagaat taagcaaaaa atatagtaag gagcaaattc taaccatgta ccttaacaac
     4201 gcttattttg gaaatggtgt gtggggtgta gaagatgcga gtaagaaata ctttggagtt
     4261 tctgcatcag aagtgagtct ggatcaagct gcgactctgg cagggatgct caaggggccg
     4321 gaactgtata atcccttgaa ttccgtagaa gattctacta atcggcgcga tactgtcttg
     4381 cagaatatgg ttgcagcagg atatattgat aaaaaccaag aaaccgaagc tgctgaagtt
     4441 gatatggctt cgcaattgca cgataagtat gaaggaaaaa tctcagatta ccgttacccc
     4501 tcttattttg atgcggtggt taatgaagct gtttccaagt ataatctaac agaggaagag
     4561 attgtcaata atggctaccg catttacaca gagctggacc aaaactacca agcaaatatg
     4621 cagattgttt atgaaaacac atcgctattt ccgagggcag aggatggaac gtttgctcaa
     4681 tcaggaagtg tagctctcga accgaaaaca gggggagttc gtggagttgt cggtcaagtt
     4741 gctgacaatg ataaaactgg attccggaat ttcaactatg caacccaatc aaagcgtagt
     4801 cctggttcta caattaagcc tttagttgtt tatacgccag cagttgaagc aggctgggct
     4861 ttgaataagc agttggataa ccataccatg cagtatgaca gctataaggt tgataactat
     4921 gcagggatca aaacgagtcg agaagttcct atgtatcaag ccttggcaga atcgcttaat
     4981 ctacctgctg ttgccgctgt taatgatttg ggtgtcgaca aggcttttga ggcaggcgaa
     5041 aaattcggac tcaacatgga aaaggtcgac cgtgttcttg gtgtcgcctt gggaagcggt
     5101 gttgaaacca accctcttca aatggctcaa gcatacgctg cctttgcaaa tgaaggttta
     5161 atgcctgaag ctcattttat tagtagaatt gaaaatgcta gtggacaagt tattgcgagt
     5221 cataaaaatt cacaaaaacg ggtgattgat aagtctgtag ctgacaagat gaccagtatg
     5281 atgttgggga ctttcaccaa cggtaccggt attagttcat cgcctgcaga ctatattatg
     5341 gcagggaaaa ctggaacaac tgaagcagtt ttcaatccgg agtacacaag tgaccagtgg
     5401 gtaattgctt atactccgga tgtagtgatt agccactggc ttggctttcc taccactgat
     5461 gaaaatcact atctagctgg ctctacttca aacggtgcag ctcatgtctt tagaaacatt
     5521 gccaatacca ttttacctta tacgccagga agtaccttta cggttgaaaa tgcttataag
     5581 caaaatggaa ttgcaccagc caatacaaaa agacaagtac aaaccaatga taatagccag
     5641 acagatgata atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga tgaggctagc
     5701 cgggctatct cagatgcgaa gattaaggaa aaggctcaaa caatatggga ttcgatagtc
     5761 aatctatttc gctaagatgc ttgtcaaagc ctagctttct tgttataatg gataggatgg
     5821 aggcgttatg gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt cgagaaacta
     5881 ttcaatcaag atcagcggaa accccaagcc tacacgacta gaagtaaata aattttgtaa
     5941 gcattgtggc aagtacacta cacacagaga aacgagatag gagagagcga tgcgttttat
     6001 tggagatatt tttagacttc ttaaagacac aacgtggcca actcgcaagg aaagctggag
     6061 agattttcgt tctatcatgg aatacacagc tttctttgta gtaattattt acatttttga
     6121 ccagttgatt gtttcaggtt tgattcgatt tattaacatt ttttagaaga ttagtggagt
     6181 taattacact agaaatcttc tatattatga aaggaaatat catggatagt tttgataaag
     6241 ggtggtttgt tttacaaact tattctggtt atgaaaataa ggtgaaagaa aatctattac
     6301 aacgtgcaca aacctacaat atgttggata atattctacg cgttgaaatt ccaacacaaa
     6361 cagtgcaagt tgaaaaaaat ggaaagagaa aagaagtaga agaaaatcgc tttccaggtt
     6421 atgttcttgt agaaatggtc atgacagatg aagcttggtt tgttgttcga aacacaccaa
     6481 atgttacagg atttgtcgga tcacacggga acagatcaaa accaactcca ttattggaac
     6541 aagaaattcg tgacattttg gtatctatgg gacaaactgt tcaagaattt gatttcgatg
     6601 ttgagattgg tcaaactgta cgtattattg atggtgcttt tgcagactac actggtaaga
     6661 ttacagaaat tgataataac aaagtgaaaa tgattatctc tatgtttggt aatgacacag
     6721 ttgcagaagt aaacctaaac caaatcgcag aattataacc ctgagagagg ctggagcctc
     6781 tcttttttgt gcagtttagg agctaaaggg aacagaatgg agaaaatgta acaaatgtgt
     6841 tttctaatct gttagactgt atctagaaag gggaaaatta tgattaaaga attgtatgaa
     6901 gaagtccaag ggactgt
//
LOCUS       DAVBCB010000082         6834 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVBCB010000082 DAVBCB010000000
VERSION     DAVBCB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.082"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(201..1625)
                     /locus_tag="VQU28_001982"
     CDS             complement(201..1625)
                     /locus_tag="VQU28_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HET2477274.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMLKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2169..4448)
                     /gene="pulA"
                     /locus_tag="VQU28_001983"
     CDS             complement(2169..4448)
                     /gene="pulA"
                     /locus_tag="VQU28_001983"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HET2477275.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4560..6518)
                     /gene="ligA"
                     /locus_tag="VQU28_001984"
     CDS             complement(4560..6518)
                     /gene="ligA"
                     /locus_tag="VQU28_001984"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HET2477276.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6611..>6834)
                     /locus_tag="VQU28_001985"
     CDS             complement(6611..>6834)
                     /locus_tag="VQU28_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HET2477277.1"
                     /translation="FFTQVQTKVESEFLGRVLSTIFTLAILFMPIAKGFMTVLPSVHL
                     SSFLIIGSGVIILSCISFIYVRTHFEKLI"
BASE COUNT         2022 a         1502 c         1239 g         2071 t
ORIGIN      
        1 actatatata gtaaaatgaa ataataacag gacaaatcga tcaggacagt caaatcgatt
       61 tctaacaatg ttttagaagt aaatctgtac tattctagtt tcaatatact ataataccaa
      121 aaagttcgta ttttttctat aattattatt attttatagc aaaaaataaa accaggaaaa
      181 caatttcctg attttacacg ttatttcaca tcaaaaacaa tggatttgac atttgtcatc
      241 gcttcaatgc tatatttaat tccttgcact ccagctccag aacctttgac accaaggaat
      301 gggaaattat ctggaccacg ctgggtttta ttattaatgt ggactgtacc tacttcaagt
      361 ttttcagcaa tttcaaaggc ttttttgaaa tcatttgtaa agactgatga ttgaaggccg
      421 aattcagatt cgttggcaaa ggcaatagct tcctctacac tagccacacg aatgattggt
      481 aaaacaggac caaatggctc ttcccatgcc actttcatat cttttgtaac ttggtcaaaa
      541 agcactggcc agaggagatt gccctcacgt ttgattggtg taagagcctg agcctctttt
      601 tcttgtgcat cctcaatcaa gccccaaatg aagtcggctg aagcattgtc aataacaggt
      661 gtaatatcag cattgtcaaa tggatcacca actgttaatt tagaaacttc ttcctgaagc
      721 aaagtagcta atttatctgc tacactttcg agaacaatga cacgtttaat ggccgtgcaa
      781 cgttgtcctg agtagctaaa ggctcccgca acaatttgct tggcagcatg ttccaaatct
      841 gcatcttcta gtacaagagc tgcatctttc ccaccaagtt ccaacatgat aggacgcata
      901 ccagctaaac gaccaatacg ttctccaata ggagttgaac ctgtaaagtt gatgaagttg
      961 acttctttgt gctcaatgat ataatcccca atttctgaac cacgacctgt aatggtgttg
     1021 aaaacacctg ccggaatccc tgcttcttca aatgctttag ccaacaagag tccagaaatg
     1081 gaaccttgtg ttggtggctt caacatgacc acattccctg caatcaaggc aggtgcaatt
     1141 ttagaagcag ataaattaac tggataatta aagggagcaa tcgctagcac gataccaact
     1201 ggttcacgac ggacaacagc cagtttgttt ttacttgctg cctcaaaacc accaccttcc
     1261 attgcttgtc cagtgatacg gagaccttcc tcagcagcat aacgaatcaa gtctgctgta
     1321 cgcactactt ctccaattgc tgctttaatc ccttttgcta cttctttggc aaggatagta
     1381 ccaatttttt ccttatcgcg ttctaaaata gctgctgttt tatgcaaata agccgcacgt
     1441 tcaattgctg ataaagctcg ccatgctggc agggctgcac gcgcagcttg catagcctca
     1501 tcagcttcag tctgagtcat ggctggaact gtacccaatt cttcttgatt gattggtgaa
     1561 taaatcgtaa tttcttgttc agatgatttc cattttccat ttactaaatt ctgatatctt
     1621 gtcaaattct tgtcctcctg aaaatgatta agatatattt taccaaattt tcagaaaata
     1681 acaacccttt ttgcaaaatt tttgagatta ttttcacaag cttgattttt caaagtatac
     1741 tcaataaaaa taaaaaaatc cactacgtca aggcgaggct aatgtggttt gaagaaattt
     1801 tcgaagagcg tgaatgagta tcatctatag taaaataaaa aaactgaaca atttggttgg
     1861 ggacagccaa accaatttct cacaatgttt cagaaacaag ggtgtgctat tccaatttca
     1921 gcctactata actgtcatag attgctgaaa caaagtctag gtaaaagtct tcataataaa
     1981 aagacctcct atcaaatgtt caaaaacttt gataggaggt cttgtttcgt gaaaatattt
     2041 atcaaatttt ctatacaagt gagctgttag ccaggttctt tctattcttt caatttcaat
     2101 gaatggattt tttaccaata ctcataactg ggaatttttc tgtctaaaaa tagcgagata
     2161 gatggtattt ataaaacact caagacagct agactaatat catttaaaac attatcttct
     2221 tttgagcgac tgttggttac caacatagct aaatttcctg cattttcaaa ttgatagggt
     2281 tctgatttag cattcacaac taccaagagg tgttctttgc cgtgaacttc atagataagg
     2341 cagccgctat gttcaatcgc agaatgcaca aagacatgat ggtaaatttc atcatagcta
     2401 gagtaagaaa aggcaccagt ttttgtcttc aatcggatga cttgacggat aaactcaata
     2461 ctgtcttgac gctcattaat caagttccag ttcacttggt tcacactgtc aggagcatta
     2521 tagctattca tcgcacgctc tctatcatca tgggtcaact caccattttc accagtcgca
     2581 accagtttgg tacgaccaaa ttcttgaccg atttccataa aggccatccc ctgcatgagc
     2641 agattcatgg ctgtggcagt ttcgaccttg cgcatgattt gctctgaact ttggtctgga
     2701 tgaagggttg ccaataaatc gtgaagattg taattgtcat gggcttctac atagttaagc
     2761 acctgatttg gatgtgtata gcttcctaat tcacgacttc ctaggattgc tttagctaga
     2821 attggctctg tcgcagcacc actgacaaaa cctgacttga tagcaccata aacttctccc
     2881 cctttgacag catcgcgctg attgtcatta aagaaaccaa tatttggcat ctggtaggca
     2941 ttgtccttct tggccttatc ataaggggca agacctgttc ccatatccca tccttctcca
     3001 tagaggataa tgttggagtc aatttcatcc aagctttgac gaatcatctg catggtcttg
     3061 acatcatgaa tccccatcaa gtcaaaacgg aagccgtcaa tattatattc ctgcacccag
     3121 tatagaagag aatcaatcat atacttgcga aacatttcgt gttcactggc tgtttcattt
     3181 ccaacacccg ttccattctg gaaggtacca tctggattca tacgataata gtaatcaggg
     3241 actgttgttt ggaatggtgc atcaacaact gagaaggtat ggttatagac tacatccata
     3301 atgactccaa tacccgcatc gtgataagct tgaaccatca ccttcaaatc acgaatgacc
     3361 tgagctggat catctggatt agttgaaaaa ctagtttctg gcgcgttata gttttgtgga
     3421 tcataacccc agttgtaggt tacatttcca tcctcatcgt attctttatg acggtctgca
     3481 attggttgca attgaacata attgtagccc agcttcttga tgtaatcaaa agcagttgac
     3541 tggccgtatt ggttaactgt tccagcctga gcagcaccca agaaagttcc tcgaagatgt
     3601 tcatctacac ccgatgtagg tgatttagtc aaatcacgaa tatgcatttc acagataact
     3661 gccttacatg gattttccaa gcgccaagta gcctccgaac cgtgcttaac ctcgaagttt
     3721 tcaacttgct tttctacatg gctcagaata gctgaacgtt tgccatcagg gctggtcgcg
     3781 attgtataag gatcacgtgt cagtgtttgg tgatgaggga attggacttg atactgataa
     3841 gtcttaccta ccaaatcttc ttcaacatcc aaactccaga caccgattgt attgtcctta
     3901 tgattataag agtagctatt gcctcttttc atctcaaaag tcttccaaac gggtgcatca
     3961 ttagcagctg attcataaac gacaacttgc acttctgtcg ctgtaggtga ccagagggaa
     4021 aaatgagcct gattgtcctc tacacggcaa cccaattctc cttggtaacc ccaatgatga
     4081 tcaaaactag cactgttaat ggccttatca aaggcaaaag gattttgatt tttatagaaa
     4141 ggactggcaa tagcaggatt ttcagagtaa taaatcctat catcgccttc caaaatccag
     4201 acctctgtta ataggggata gtgattaaaa cggatagaat attctttact agtttgacct
     4261 gtatgaacca caaaattcaa gctttctata acatgtgaac ttgggtgttc aaagctaaat
     4321 aaagctccaa aataatcttc tttgtaggtt agcaaatcaa ttcgttgatc ctgacttttt
     4381 acaaaggagc aagtgtcata ttctccattc ttacgatggt aatgaatgcg catagggtag
     4441 ttatacattt tttatttttc ctttttactt tgtttctatt tcactaataa atttttgtca
     4501 atctcgtctc aattaacaga catagtcata ttctctaaac tctgttttta aacgatccat
     4561 tacaaacttt ctagccatgc ctcatctctg acctggatac caagttcttg tgctttttgc
     4621 agtttacttc cagcgtctgc acctaccacg acgaggtcgg tctttttaga aacactacct
     4681 gtcactttgg cacccagact ttcgagttta cttttagctt ctgagcgctt gagtcgttcc
     4741 aattttcctg tcaataccac ggtcaaacct gacaaggccg catccgctac taccgtctgt
     4801 cctttatagt ccagattgac cccagtttct ttcaattctc tgagcagaat ttcagagcct
     4861 tctgtcgcaa aataagtctg aagacttttg gcaatcacgc cacctagact ttcaatacta
     4921 gccacttcct ctgaatctgc ctgagacaga ttttcaattg aatggaaata ttgaagtaaa
     4981 agctgactag ccttgcttcc gacatgacga attcccaaac caaataagag cttctcggca
     5041 gaattttcct ttgatgcttg gatagcctga tacagtttag cagcggactt ttccttaacc
     5101 ccctctaaaa ggaggaaatc ctcttcttgc aaacgataaa tatccgccac atccttgact
     5161 aaattagcag caaaaagctt ctcaacaata gatggaccaa ggcctgtaat attcatagca
     5221 tcacgagaag caaagtgaat caagccttcc atgatttgag cagggcaacg cggattgata
     5281 caacgtaggg ccacttcatc ttcaaagtgc aacaagtcag agttacaact tggacagttt
     5341 gtagggatat ctagtttttc ttcagaaacc cgtttggact ctaccacacg taaaacggca
     5401 gggatgatgt caccagcctt atatacaatg accgtatcgt cttttcggat atctttttca
     5461 gcaatataat ctacattgtg cagggtcgca cggctaacag tcgtaccggc aagttgtact
     5521 ggtgttagat tagcagttgg agttacaaca ccggtacggc caactgtcca gtcaactgat
     5581 aagagttgag cttctttttc ttcagcaggg aacttgtagg ctactgccca ctttggagcc
     5641 ttaacggtaa aaccaagttc ttcttgactt gctaggtcgt tgaccttgat taccactcca
     5701 tcaatatcgt aaggcagatt ttcccgttcc tgtcctactt cttggataaa attccagatt
     5761 tcatctatgt tttcagccaa gattcgctta ggattgacca caaaacctag ttgttctagg
     5821 tacttcaaac ccttttcttg gctatcacga gttgaagggc tggcttcttg atagagaaac
     5881 gttgcaagat tacgcttggc aactactgct gtatccaact gacgcagagt tcctgctgcc
     5941 gcattacgag gattagcaaa ttcaggctct ccattttctt ggcgcacttg gttaacttgg
     6001 tcaaaggaag cgcgtggcat gtaacattcc ccacgaactg tgatatctag ttcttctggc
     6061 aaagtcaaag ggatgtcctt aacacgcttg aggttttctg tgatattttc accaattgaa
     6121 ccatctccac gtgttacccc agcaaccaaa atcccctttt cataagtcag cgagatagat
     6181 aagccatcga ttttcagctc acaaatatag gtcggatgag ccacttcctt acgaacacgc
     6241 gcatcaaaag catctagctc ctcacgtgaa aaagcatcct gcaaactata aagaggatac
     6301 tgatgactgt atttttcaaa accatctaaa accttgccac caacacgatg agtcggactg
     6361 tctgctagca cttgctctgg ataagcagtt tctaactcga ccaactcacg gtaaaggcgg
     6421 tcatactcac tgtctgaaac cgagggatta tcgctggtat agtactcagt cgcatagcga
     6481 ttgagcaaag cgactaactc attcattctt ttattcataa gaccatttta ccataaaaca
     6541 agccctcctc acaaacgaga agggcggaaa aaacacttag tttgaaatta tttttgaaac
     6601 tcaagcaacc ttatatcaat ttttcaaaat gagttcgaac ataaataaac gatatacaag
     6661 acaagatgat aacaccactt ccaattatca ggaaagaaga gagatgtaca cttggcaaga
     6721 ctgtcataaa tccttttgca ataggcataa atagaatagc taaggtaaaa attgtactca
     6781 gtactcttcc aagaaattcg ctctcaacct tggtttgtac ttgagtaaaa aagt
//
LOCUS       DAVBCB010000083         6637 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVBCB010000083 DAVBCB010000000
VERSION     DAVBCB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6637
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.083"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(84..1244)
                     /locus_tag="VQU28_001986"
     CDS             complement(84..1244)
                     /locus_tag="VQU28_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HET2477278.1"
                     /translation="MKKKFALSFVALASVALLAACVEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTNMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            complement(1345..1593)
                     /locus_tag="VQU28_001987"
     CDS             complement(1345..1593)
                     /locus_tag="VQU28_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HET2477279.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQU28_001988"
     CDS             complement(1672..2262)
                     /gene="clpP"
                     /locus_tag="VQU28_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HET2477280.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQU28_001989"
     CDS             complement(2436..3065)
                     /gene="upp"
                     /locus_tag="VQU28_001989"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HET2477281.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETFQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3154..3621)
                     /locus_tag="VQU28_001990"
     CDS             complement(3154..3621)
                     /locus_tag="VQU28_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HET2477282.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            complement(3640..4197)
                     /locus_tag="VQU28_001991"
     CDS             complement(3640..4197)
                     /locus_tag="VQU28_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HET2477283.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            4322..5167
                     /locus_tag="VQU28_001992"
     CDS             4322..5167
                     /locus_tag="VQU28_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HET2477284.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            5226..6398
                     /locus_tag="VQU28_001993"
     CDS             5226..6398
                     /locus_tag="VQU28_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HET2477285.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSNPI"
     gene            6449..>6637
                     /locus_tag="VQU28_001994"
     CDS             6449..>6637
                     /locus_tag="VQU28_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HET2477286.1"
                     /translation="MEHIILLRGVTPNGKNAIPKMSYLVDILTEADFQQVRTYIQSGN
                     IILESNLALEEIREQVHTL"
BASE COUNT         1894 a         1385 c         1287 g         2071 t
ORIGIN      
        1 agtttgaaga gattttcgaa gagtattaaa tatcgaaaca gggagtgagt caaaggctca
       61 ttccctattt caacattttt ctattatggt tttacaactt ctgctgcttc aactttacca
      121 ttgttcatgg tcatcatgta agcagttttg actgtgttgt ggtctgcatc gaagcttgtt
      181 tgaccagtta caccttcaaa atcttttgtt ttagcaaggt tatccttgat ttcacctgaa
      241 tttttagcac cttttgctgc gtttgctaca aggtgaactg aatcataagc caaggctgca
      301 aatgttgaag gctcttcatt gtacttagca cggtaagcgt caaggaaggc tttagcttta
      361 gctgaaactt ctacagtagt tgagaagcct gagataaagt agatgtttga tgctttttca
      421 gcagttgctt gttgtacaaa ctcctcaccg ttgaatccat caccaccaac gattggtttg
      481 tcaattccca tgccacgcgc ttggtttaca atcttaccag cctcattata gtaaccagga
      541 acaacgatag catcaaagtc tttccctttc atgtttgtaa gggctgcttg gaagtctgtg
      601 tcacctgcta cgaaagtttc atctgcaacg atttcaccct tgtatgactc gcggaaagat
      661 ttagcaatcc ctttagcata gtcactggca ttgtcagtgt aaagaacaac tttcttagca
      721 tttaattttt cagaaacata gtttgagata atttttcctt ggaagctatc ttggaaagtt
      781 ccaataaaga ggtaatcttg acctttagtc aatccatctt gagtcgcact tggtgagatc
      841 aatggaacac ctgcttttgt agcgttcgct accgcagctg cagtcgcacc agatgtcgca
      901 ggtcctacga ctgctgatac tttagattgg gttacaaggt tagttgtaac tgaagcagcc
      961 tcagctgttt cagacttatt atctttatcg actacttcga tttgttttcc atcgatacca
     1021 cctgcggcat tgatttcatc aacagccaat tgggcacctt tttgttcagc tgttccgtat
     1081 gcagctaaag aacctgattc ttcaaagtta aacccgattt taattgtctt ttcctctact
     1141 gagttaccag cagtgttgac tgctccagac ttcacttcta cacaggctgc aagaagtgct
     1201 acacttgcaa gcgccacaaa cgatagggca aattttttct tcatcttttt gtctccttat
     1261 ttcttaaata gtctgttaag aatataccga attatcagaa aattgtcaat gttttccttg
     1321 aactttttcg atgataacgt tttcctatcg ataaagattg cccacaaagg gtgtttccaa
     1381 ctcttggata tgacaaaccc taactttttt aataaatttt tccttgctca agcgtccgac
     1441 caattgctcc acttcttgag ttggaacata gagttgtaag taacgatgtt tcttggaatg
     1501 ataggtaata tctccataat cctgcagttt tttggcatca cgattatagt aaagatagat
     1561 aatcaagcca gagcgattga ctttttcaaa catgataaat cctctttcta agaaatctct
     1621 ccctattata ccaaaaaaag caaacccagt cgagtttgcc ttctatcatc attagttcaa
     1681 tgaattgttg gccataattt catcaataaa gccatattca agtgtttcct gggcgctcat
     1741 ccagttatca cgttctgcat ctgcatggac tttttccatt gactgacctg aattttcagc
     1801 caagattttt tccaaggtat tacgagtttt gagcaagtgt tctgcagcga tagccatatc
     1861 agtttgttgg gtaccaccac ctgtaccgcc cattggttgg tgaatcatgt attcagcatt
     1921 tggaagcatg aaacgtttgc cttttgctcc acttgatgcg atgactgttc ccatagatgc
     1981 agccattccc ataacaatgg tttggacatc tgccttgata aagttcatgg tatctacgat
     2041 tgccaaacca gctgaaacag aaccacctgg tgtattgaca taaaggtaaa tatcttttgt
     2101 actatcttgg gcatccaaga aaagcagttg ggcaataaca gagttagcca tattgtcttc
     2161 aaccggacct gtcagcataa tgatgcggtc tttgagaaga cgtgagtaaa tatcgtaaga
     2221 acgttctcca cggcttgttt gttcaataac tacaggaatc attcatttct ccttttgagt
     2281 tttaattttg ttggtcaaat gactgaagat aagactatta taatatcttg gtcaaaaaag
     2341 gtcaaatttt tgctctgctt tcattagaca gaaacaaaaa cccaacctcc tttcgtgact
     2401 ggaaatactt ttccaagtca ttcttctttt cgatcttatt ttgtaccgaa caagcggtct
     2461 ccagcatctc caagacctgg aacgatataa ccgtgttcgt tcaaacgttc atccaaggct
     2521 gctgtaaaga tttctacatc tggatgagct tcttgaaggg cttttacacc ctctggagca
     2581 gatacaaggc agacaaattt gatatttgat gcgccacgtt ttttaagaga atcaacagcc
     2641 aagattgctg agccacctgt tgccaacatt gggtctacta caaaaatttg acgttggtca
     2701 atgtcctcag gcaatttcac caagtattca actggttgaa atgtttcttc atcacggtac
     2761 ataccgatgt ggccaacttt agcagctgga accaagctca agagaccatc aaccatcccg
     2821 atacctgcac gcaagattgg gacgatggcc aatttcttac ctgccaattg tttttgaact
     2881 gtttttgtaa ttggtgtttc gatttccaca tcttctagtg gaagatcacg aagtacttca
     2941 taccccatca acattgcaat ctcatctact agctcacgaa aagcttttgt agaagtatct
     3001 gtacgacgca agattgacaa tttgtgttga atcagtgggt gattaataac ttcaattttt
     3061 cccatttttg gaattccttc tttcaattta ttcttcttat tataccaaaa aacgggttaa
     3121 aaatctttct aaaccattta tttttgataa tttttacatt agatcagcct ctttaagagc
     3181 tgtctgtact gtctcaagtg gtaaatgggt caattctgtc cctttttctt gataaaggta
     3241 ttgggcgtag tcgtccattc ggtactggtt gatataaacc acacgcttgc agccgacctg
     3301 aagcaattgt tttgtacagt tgagacaagg aaaatgggtt acataggctg taaagccttt
     3361 gggaacacca cgctcagcac cttgaaggat agcattgacc tcagcgtgaa gggtgcgaac
     3421 acagtggcct tcaatgacca aacattcgtg atcaatacaa tgctcagtcc ctgacaccga
     3481 accattgtaa ccagtggaaa taaccttatt atcttttacc agaatcgcgc ccactttagc
     3541 acgtttacaa gtggaacgat tcgcaattag tagagcttgg gctgcaaaat actcatccca
     3601 ggccagtctt ttttcagtca tctcttttct cctttttctc tattttttaa aaaatggtaa
     3661 acctaaatct gcaatctttt cagctggtac cttcatgcca tccttgatcc attttagaag
     3721 gacagagacg atggctgagc tccagaagga atgaagataa gagctgacac cttttgattt
     3781 cccatggtat ttttctagaa attcctgcat ggcttggaca aagatttttt ccagatggta
     3841 atccaaggcc aattgaatta ctctagcttc ctttctggcc tcccggaaaa ggtgaaccca
     3901 aaccaaataa aggtctgtct ttaaatcgta atgatgcagc tgttccataa tattgtggac
     3961 agttcgttta aagacgctct ctaaaatttc ctctttggag tcataattgc gataaaaggc
     4021 cgcacgcgaa acacctgcac gtttgaccaa ttcagaaata ctaatcttgg tcagttcctt
     4081 tttttccaag agttgcaaga gggctgtttc aatggcttct ctggttaata aattggattc
     4141 ttggtttgat tttctgagat tttcaagaga cttttcagag attctacgtt cagacataac
     4201 attttctttc tacttgtcac aacagacgga tgatgctttt gtttcaaggg ttttcatgat
     4261 ataattgtaa aaaaagacag aacctttgtc aatgtataac tgacaaagat ggagaatttc
     4321 tatgacttgg aagattattg ctgactctgg ttgtgattat cgtcaactgc caacaccagc
     4381 tattaacaca acctttgtaa gtgtcccctt aaccattcaa gtagctgatc aggtctttgt
     4441 tgatgacgcc agtctcgata ttgaccaaat gatggaaacc atgtatgcaa ctgcagaagc
     4501 ttcaaaatcg gcttgtccaa gcccagatga ctatttgcga gcatttgaag gtgccaaaaa
     4561 cattttccta gtaaccatca caggtaccct ttctggcagt cacaatagtg ctcaactagc
     4621 aaagaatatt tatctggaag accatcctga cactaagatt catgtgattg atagtttatc
     4681 tgctggtggt gaagttgacc tactcgtaga aaaattgaat gacttgattg accagggctt
     4741 gtcttttgaa gaagtggttg aagctatcac cgcctatcaa gaaaaaacta agttgctctt
     4801 tgtcctagcc aaagtcgata acttggtgaa gaacggccgt ttgagcaagc ttatcggtac
     4861 ggtcgttggc cttctcaaca ttcgtatggt cggaaaagct agtgaaactg gaactctcga
     4921 attgctacaa aaagcaaggg gatcaaagaa atcagttcaa gctgcctatg atgagttagt
     4981 aaaagctgga tatgctggtg gccgtattgt catggcccaa cgcaataacg aaaaatgttg
     5041 tcaacagctc tcagagcgaa tccgtgaaac cttcccacaa gcggatatta aaattctacc
     5101 aacctctggt ctctgcagtt tctatgcaga agatggcggt ttgctgatgg gctatgaaat
     5161 tgattaattg tattcttgac aagaggtgac catcatctct tgtttttttg tggtacaata
     5221 gagctatgaa acattttgat actattgtca tcggtggggg acctgctggt atgatggcta
     5281 cgatttccag tagcttttat ggacagaaaa ccctcctcat cgaaaaaaat cggaaacttg
     5341 gaaaaaaatt agctgggact ggtgggggac gttgcaatgt gaccaacaat ggtagcttag
     5401 acaacctgct agctggaatt cctggaaacg gacgctttct ttacagtgtt ttctcccagt
     5461 tcgataatca tgacatcatc aactttttta cagaaaatgg tgttaaactt aaggtcgaag
     5521 accacggacg cgtctttcca gccagtgaca agtctcggac tattatcgaa gctttggaaa
     5581 agaaaatcac cgaactaggt ggtcaagttg ctactcaaat agaaatcgtt tctgttaaaa
     5641 aagtagatga ccagtttgtc cttaagtcag cggatcaaac cttcacttgt gagaaactca
     5701 ttgtcacaac aggtggtaag tcttatcctt cgactggttc gactggtttt ggtcacgaga
     5761 ttgctcgcca ttttaagcat accatcaccg atcttgaggc tgctgaaagt cctttattaa
     5821 cagattttcc acataaagcc ttacaaggga tttctttgga cgatgtgacc ctaagttatg
     5881 gtaagcatgt catcactcat gatttactct ttacccactt tggtttgtca ggtcctgctg
     5941 ccctacgcat gtctagcttt gtcaaaggtg gggaggttct ctcactcgat gttttgcctc
     6001 aactttctga gaaggacttg gttacatttc tagaagaaaa tcgggaaaaa tccttgaaaa
     6061 acgctttaaa aaccttgtta ccagaacgct tggccgaatt ttttgtacaa ggatatcctg
     6121 aaaaagtcaa acaactgact gaaaaggaac gagaacaact tgtccagtcc attaaagaac
     6181 ttaaaattcc tgtaactgga aaaatgtccc ttgcaaagtc ctttgttacc aagggtggag
     6241 tcagtctcaa ggaaatcaat cctaaaaccc ttgaaagtaa gctggtacct ggcctccact
     6301 ttgcaggcga agttatggat atcaatgccc acacaggtgg ctttaacatc acttctgccc
     6361 tctgtaccgg ctgggtggcg ggatcaaatc caatctaaat atacattact aaatggaaca
     6421 atcccccaaa aaaaagaaag gaactacttt ggaacatatt atcttgctaa ggggagtcac
     6481 tcctaatgga aaaaatgcta tccctaaaat gtcttatcta gtagatatct tgacagaagc
     6541 tgattttcaa caagttcgaa cttatattca aagtggaaat attattcttg aaagtaactt
     6601 agctctcgaa gaaatacgag aacaggttca tactcta
//
LOCUS       DAVBCB010000084         6498 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVBCB010000084 DAVBCB010000000
VERSION     DAVBCB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6498
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.084"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..154)
                     /locus_tag="VQU28_001995"
     CDS             complement(<1..154)
                     /locus_tag="VQU28_001995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477287.1"
                     /translation="MSLITHRRFISCNEIIKHYKRLIDKAEKCVNDLMAEFNSVITTV
                     TGIGNRL"
     gene            307..2073
                     /gene="patB"
                     /locus_tag="VQU28_001996"
     CDS             307..2073
                     /gene="patB"
                     /locus_tag="VQU28_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HET2477288.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTHVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2212..2901
                     /locus_tag="VQU28_001997"
     CDS             2212..2901
                     /locus_tag="VQU28_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HET2477289.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLDEL"
     gene            2905..3084
                     /locus_tag="VQU28_001998"
     CDS             2905..3084
                     /locus_tag="VQU28_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477290.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3071..4420
                     /locus_tag="VQU28_001999"
     CDS             3071..4420
                     /locus_tag="VQU28_001999"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HET2477291.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4544..6004
                     /locus_tag="VQU28_002000"
     CDS             4544..6004
                     /locus_tag="VQU28_002000"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HET2477292.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6018..6452
                     /locus_tag="VQU28_002001"
     CDS             6018..6452
                     /locus_tag="VQU28_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HET2477293.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNTYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2036 a         1251 c         1397 g         1814 t
ORIGIN      
        1 ctaaacgatt cccaatccca gtaaccgtcg tgatgaccga gttgaactca gccatcaagt
       61 cattgacaca tttttccgcc ttgtcaatga gcctcttgta atgtttgatg atttcattac
      121 acgagataaa acgtctatgc gttatcaaac tcattaccaa ttaaaacaaa aagctgtggt
      181 tagatccttt cggaaatcgt caagcgattg gaggaaatga actaatccac agcggcttat
      241 tccaagtata ccacttgggc tttggcagta gctaactgcg ctaaatataa tataaggagg
      301 agtaaaatga agacagttca atttttttgg cattatttta aggtctacaa gttctcattt
      361 gtagttgtca tcctgatgat tgttctggcg acttttgccc aagccctctt tccagtcttt
      421 tctggacaag cggtgacgca gctagccaat ttagttcaag cttatcaaaa tggcaatcca
      481 gaacttgtat ggcaaagcct atcaggaatc atggtcaatc ttggcctgct ggttttggtt
      541 ctatttatct ctagtgtaat atacatgtgt ctcatgacgc acgtgattgc agaatcgacc
      601 aacgagatgc gcaaaggcct ctttggtaag cttgctcagt tgacggtttc tttctttgac
      661 cgtcgacaag atggcgatat cttgtctcat tttaccagtg atttggataa tatcctccaa
      721 gcctttaacg aaagcttgat tcaggtcatg agcaatattg ttttatacat tggtctgatt
      781 cttgtcatgt tttcgagaaa tgtgacgctg gctctcatca ccattgccag caccccattg
      841 gctttcctta tgctgatttt catcgtgaaa atggcacgca aatacaccaa cctccagcag
      901 aaagaggtag ggaagctcaa cgcctatatg gatgagagca tctcaggcca aaaagccgtg
      961 attgtgcaag gaattcaaga ggatatgatg gcaggatttc ttgaacaaaa tgagcgcgtg
     1021 cgcaaggcaa cctttaaagg aagaatgttc tcaggaattc ttttccctgt catgaatggg
     1081 atgagcctga ttaatacagc catcgtcatc tttgctggtt cggctgtact tttgaatgat
     1141 aagtctattg aaacaagtac agccctaggt ttgattgtta tgtttgcaca attttcacag
     1201 cagtactacc agcctattat ccaagttgca gcgagttggg gaagccttca gttggccttt
     1261 actggagctg aacgaattca ggaaatgttt gatgcagagg aggaaatccg acctgaaaag
     1321 gctccaacct tcactaagtt gcaagaaagt gttgaaatca gtcatatcga tttttcatac
     1381 ttgcctgata aacctatttt gaaagatgtc agcatttctg cccctaaagg ccagatgaca
     1441 gcagttgttg ggccgacagg ttcaggaaaa acgactatta tgaacctcat caatcgcttt
     1501 tatgatgttg atgctggtgg tatttatttt gatggtaaag acattcgtgg ctatgactta
     1561 gatagtctta gaagcaaggt gggaattgta ttgcaagatt cggtcttgtt tagcggaacg
     1621 attcgagaca atatccgatt tggtgtgcca gatgctagtc aggaaatggt tgaggtagca
     1681 gcaaaagcaa cccacattca cgactatatc gaaagtttgc ctgataagta cgatactctt
     1741 attgatgatg accagagcat cttttcaaca gggcagaagc aattgatttc aatcgctcga
     1801 accctgatga cagatccaga agttctcatt ctcgatgaag caacttcaaa cgtagatacg
     1861 gtgacagaaa gcaagattca gcatgccatg gaggtggttg tagcaggtag aactagtttc
     1921 gtcattgccc accgcttgaa aaccattctc aatgcagatc agattattgt ccttaaagat
     1981 ggagaagtca ttgaacgtgg taaccaccat gaacttttga agctaggtgg cttttattca
     2041 gaactctatc acaatcaatt tgttttcgaa taagaaagaa gttgtcctat gtgggcagct
     2101 ttttcttgtc cataaaaaat gtttatcaca gccttaaaaa aaacatatta gacgaaagtc
     2161 attttgagtg atatgatagg actatcgtta gcattcgaaa ggagaggcat catggctaga
     2221 acggttgtag gagttgctgc aaatctatgt cccgtagacg cagaaggcaa aatcattcat
     2281 tcatctgtat cttgtagatt cgcagagatc attcgtcaag tcggtggtct ccctttagtc
     2341 attcctgttg gtgatgagtc agttgtacgt gattatgtgg aaatgattga caaactcatt
     2401 ttgacaggag gccaaaatgt tcatcctcag ttttatggag agaaaaagac cgtcgagagc
     2461 gatgattaca atctggtccg tgacgaattt gaattggcac tcttgaagga agcgcttcgt
     2521 cagaataaac caattatggc aatctgtcgc ggtgtccaac ttgtcaatgt tgcctttggt
     2581 ggaaccctca atcaagaaat cgaaggtcac tggcaaggac tacctttcgg gacatctcac
     2641 tctattgaga cagtagaagg aagcgtggtg gctaagctat ttgggaaaga aagtcaggtc
     2701 aactcagtcc atcgtcaaag cattaaagat ttggcaccta atttccgtgt aactgctatt
     2761 gattcaagag accaaactat tgaagcgatt gagtctatcg acgagcaccg cattatcggt
     2821 ttacagtggc atccagagtt tctggttaat gaagaagatg gcaatttaga attatttgag
     2881 tatttattgg atgaactgta acaattggaa catctggtcg ttctttcttt tattttttca
     2941 aaatttttag aatgtggtat tttacgcaaa cgtttgaatt ctgataaaaa ttggagaaat
     3001 tcgacaaaaa aacttgaaaa aaacgaaggt aagcgttatg atagaaaaga agaaatattg
     3061 gaggaagaac atgtcacata ttaaatttga ttattcaaaa gttttagaca aatttgttgc
     3121 accacatgaa gtggaataca tgcaatcaca agtaacagca gcagatgaat tgatccgtaa
     3181 aggaactggt gctggtagcg acttcttggg atggttggac cttcctgaaa aatacgaccg
     3241 cgaagaattt gaccgcatct tgaaagctgc tgagcaaatc aagtcagaca gcgatgtttt
     3301 ggttgtgatc ggtattggtg gatcttacct tggtgcaaaa gcagcaatcg acttcttgaa
     3361 tcaccatttt gctaacttgc aaacaaaaga agaacgcaaa gctccacaaa tcctttacgc
     3421 aggaaactca atctcatcta cttaccttgc tgacttggta gagtatgttg cagacaaaga
     3481 cttctcagta aacgtaattt ctaaatcagg tacaacaact gaaccagcga ttgctttccg
     3541 tgtctttaaa gaactcttgg ttaagaaata cggtcaagaa gaagctaaca aacgtatcta
     3601 tgcaacaact gaccgccaaa agggtgctgt taaggttgaa gcagacgcta acggttgggg
     3661 aacatttgtt gttccagatg atatcggtgg acgcttctca gtattgacag ccgttggttt
     3721 gcttccaatc gcagcatcag gagctgacat aaaagctctt atggaaggtg cgaatgcagc
     3781 tcgcaaagac tacacttcag acaaaatctc tgaaaacgaa gcttaccaat acgcagctgt
     3841 tcgtaacatc ctttatcgta aaggctatgc aactgagatc ttggtaaact atgagccatc
     3901 acttcaatac ttctcagaat ggtggaaaca attggctggt gaatcagaag gaaaagacca
     3961 aaaaggtatc tacccaactt cagccaactt ctcaactgac ttgcactcac ttggtcaatt
     4021 tatccaagaa ggaactcgta tcatgtttga aacagttgtc cgtgttgaca aacctcgtaa
     4081 aaacgtgctt attcctactt tggaagaaga ccttgacgga cttggttacc ttcaaggaaa
     4141 agacgttgac tttgtaaaca aaaaagcaac tgacggtgtt cttcttgccc acacagatgg
     4201 tgatgtacca aacatgtatg tgactcttcc agagcaagac gctttcactc ttggttacac
     4261 tatctacttc ttcgaattgg caattgccct ttcaggttac ttgaatgcta tcaacccatt
     4321 tgaccaacca ggtgttgaag cttataaacg taacatgttt gcccttcttg gaaaaccagg
     4381 atttgaagaa ttgagcaaag aacttaacgc acgtctataa tagaagaaaa gagtggtttg
     4441 cccactcttt ttactctctt tatccataga aattggactc agccaagact tgtgatataa
     4501 tatagaaagc aaaaaggcag acgcctagat aataggagaa actatgtcaa aagatatccg
     4561 cgtacgttac gcaccaagtc caacaggact actacacata ggaaatgctc gtacagcatt
     4621 gtttaattac ttgtatgcgc gccatcatgg tggaacattt ctcatccgta tcgaagatac
     4681 tgaccgtaaa cgccatgtcg aggatggtga acgttcacaa cttgaaaacc ttcgctggtt
     4741 aggcatggat tgggatgaaa gtccagaatc acatgagaat tatcgccagt ctgagcgttt
     4801 ggacttgtat caaaaataca tcgaccaact attagctgaa ggaaaagcct acaaatctta
     4861 cgttacagaa gaagagttgg cagctgaacg tgaacgtcaa gaagtagctg gcgaaacacc
     4921 acgctacatc aatgaatacc ttggtatgag tgaagaagaa aaagcagctt acatcgcaga
     4981 acgtgaagca gcagggatca tcccaactgt tcgtttggct gtcaatgagt caggtatcta
     5041 caagtggcat gatatggtca aaggcgatat cgaatttgaa ggtggcaata tcggtggtga
     5101 ctgggttatc caaaagaaag acggttaccc aacttacaac tttgccgttg ttatcgatga
     5161 ccacgatatg caaatctctc atgttatccg tggagatgac catattgcta atacaccaaa
     5221 acagcttatg gtctatgaag ctcttggttg ggaagctcca gagttcggtc acatgacctt
     5281 gattatcaac tctgagactg gtaagaagtt atctaaacgt gatactaata cacttcaatt
     5341 tatcgaagat taccgaaaga aaggttacct tccagaagca gtctttaatt ttattgctct
     5401 tcttggttgg aacccaggtg gtgaagatga gattttctct cgtgaagaac tcattaaact
     5461 ttttgatgaa aaccgcctca gcaagtcacc agcagccttt gatcagaaaa aactagactg
     5521 gatgagcaat gattatatca agaatgcaga ccttgaaacc atctttgaaa tggcaaaacc
     5581 attcttagag gaagcaggcc gtttgactga caaggctgaa aaattagttg agctctataa
     5641 accacaaatg aaatcagtag atgagattat cccattgaca gatcttttct tctcagattt
     5701 cccagaattg acagaagcag agcgcgaagt catgacgggt gaaacagttc caacagttct
     5761 tgaagcattc aaagcaaaac ttgaagcgat gacagatgat aaatttgtga cagaaaatat
     5821 cttcccacaa attaaagcag ttcaaaaaga aacaggtatt aaagggaaaa atcttttcat
     5881 gcctattcgt atcgcagttt caggcgaaat gcatgggcca gaattaccag atacaatttt
     5941 cttgcttgga cgtgaaaaat caattcagca tatcgaaaac atgctaaaag aaatctctaa
     6001 ataaagagga tacaataatg gacatctggg aaaagatgta cgaagaagca cagaaactat
     6061 acaatccaca tgaagtatca gactttgttt atgccaatca tgttgtagct gcagtagaag
     6121 cagaagatgg acaaatattt acaggattct gtatggaggg aacctgtggt gttttccatc
     6181 tctgcgcaga acgggcggcc ctcttcaata cgtaccaatt ttcaggacaa actaaggtta
     6241 agaaagtatt agcctttcga gacaaaccac cttatggtgg aagttcagcc atgccttgcg
     6301 gtgcttgtag agaattcctt ttagagctta atgctgaaaa taaagatgca gaattcatga
     6361 tggactataa tataagaaaa acagttaaag tcgcagaact aatcccttat tggtggggag
     6421 aagaacgtgc ttctaagttt aatgagcgat agaagagtca gtataattct ggcttttttt
     6481 actataagtt gaaaaaat
//
LOCUS       DAVBCB010000085         6447 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVBCB010000085 DAVBCB010000000
VERSION     DAVBCB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.085"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            222..422
                     /locus_tag="VQU28_002002"
     CDS             222..422
                     /locus_tag="VQU28_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477294.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            447..863
                     /locus_tag="VQU28_002003"
     CDS             447..863
                     /locus_tag="VQU28_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477295.1"
                     /translation="MKAALDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETN
                     SVPAGVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKA
                     QNLLQETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQU28_002004"
     CDS             1094..1801
                     /gene="ccdA2"
                     /locus_tag="VQU28_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HET2477296.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1812..2369
                     /gene="sdbB"
                     /locus_tag="VQU28_002005"
     CDS             1812..2369
                     /gene="sdbB"
                     /locus_tag="VQU28_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HET2477297.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2490..3881
                     /locus_tag="VQU28_002006"
     CDS             2490..3881
                     /locus_tag="VQU28_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HET2477298.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPSWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4024..4941
                     /gene="adcAII"
                     /locus_tag="VQU28_002007"
     CDS             4024..4941
                     /gene="adcAII"
                     /locus_tag="VQU28_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HET2477299.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            4949..>6447
                     /locus_tag="VQU28_002008"
     CDS             4949..>6447
                     /locus_tag="VQU28_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2477300.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGNHFHYIPKSDL
                     SASELAAAQAYWNGKQGSRPSSSSSHNANPAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLSERHVESDGLVFDPAQITYRTVNGVAVPHGDHYHFIPYSQLSPLEEKLA
                     RMIAVKGQNGAVLPGMHYLKPAPKPQVQPSTEKKQTDFAVEQVVRKVGEGYVVEIAGV
                     SHYVFAKDLAKDKIDAIENLLSKKTQETHALVAKKENVAPRDQEFYDKAYNLLTQAHK
                     VLSENKGRTSDFQALDKLAERLNNESSNKVKLVDDLLAFLAPITHPERLG"
BASE COUNT         1980 a         1172 c         1354 g         1941 t
ORIGIN      
        1 atggtttcag aaatttctct taccaacatt aaccacacca tcggttatta tagtaaaatg
       61 gaataagaac aggacaaatc gatcaggaca gtaaaatcga attctaacaa tgttttagaa
      121 gtctatatgt actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc
      181 ctgtcccagc tccaagctca gctccgactc aagatcctag agtggtagca agcgcttcgg
      241 ctagctcaac gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga
      301 gtcaagagtt agaccaacgc ttgaaagctt tggcaacagt atctgatcca aaaatcgatg
      361 caaccaaggc tgtcctccta gatgctcaaa aagctccgga agatagtgcc ttgacagagt
      421 aaggtctgca tactgcagta gagtctgtga aggctgcctt ggactctctc aaagatgtga
      481 aagcaaatgc tagcgacagc aagcctgcac aggacaagaa ggatgcaaaa caaggaacgg
      541 aagatagtaa ggattcagat aagatgactg aaacaaactc agttccggca ggagtgattg
      601 tggtcagtct acttgccctc ctaggcgtga ttgccttctg gctgattcgc cgtaagaaag
      661 agtcagaaat ccagcaatta agcacggaat tgatcaaggt tctaggacag ctagatgcag
      721 aaaaagcgga taaaaaagtc cttgccaaag cccaaaacct tctccaagaa acccttgatt
      781 tcgtgaaaga agaaaatggc tcagcagaga cagaaactaa actagtagag gagcttaaag
      841 caatccttga caaactcaag taaaacatga tttatgaaat gtcagaccta aaagacattt
      901 tcgttcacaa aagcagtgac gagtaatcct tgtcgttgct ttttggctat tttgggggat
      961 aagatccgcc ttgactagca aaagaaaaga ttggtccaat agtgcccgat acgattttgc
     1021 gtagcagaaa gcttacaaat aaaattgaat atagtaaaat aggattagaa ttattaagaa
     1081 agttggttct ttattggaaa cgatagtatt tttaatctct gtttttctag caggtgtttt
     1141 atcctttttt tctccttgta tttttcctct tctgccagtc tatgctggga ttttattgga
     1201 tgatcaagaa agtgcaaaaa gcttttcttt gtttgggaga aaggttctct ggtcaggctt
     1261 gattcgaaca ctttgcttta tcgctggtat ctctctcatt ttctttattc taggctttgg
     1321 tgctggttac tttggtcata ttctctatgc aaattggttt cgatatgtca tgggagctat
     1381 tattatcatt ttgggtcttc accagatgga aatttttcat ttgaagaaat tagaagttca
     1441 aaaaagtttt acctttaaaa aatcagattc taatcgttat tggtcagctt ttttacttgg
     1501 tattaccttt agctttggtt ggacaccttg tattggtcca gttttaagtt ctgttttagc
     1561 acttgcggct tctggaggca atggtgcttg gcaaggagcg atttatactc tcatttacac
     1621 tctgggcatg gcccttcctt tcttggtctt ggcactagct tcaggtctag tcatgccata
     1681 ttttagtaaa atcaagcgtc atatgatgct actaaagaaa attggtggtt tcctcattgt
     1741 tttaatggga attttgttac tattaggaca agtaaatgtt ctagctggaa tttttgaata
     1801 aaggaggaca aatgaaaaaa gtaatgtttg ctggcttaag tctcttgtca ttagttgtat
     1861 tgatggcctg tggtgaggaa gaaactaaaa agactcaagc agcacaacag ccaaaacaac
     1921 aaacgactgt acaacaaatt gctgttggaa aagatgctcc agacttcaca ttgcaatcca
     1981 tggatggcaa agaagttaag ttatctgatt ttaagggtaa aaaggtttac ttgaagtttt
     2041 gggcttcatg gtgtggtcca tgcaagaaaa gtatgccaga gttgatggaa ctagcggcga
     2101 aaccagatcg tgatttcgaa attcttactg tcattgcacc aggaattcaa ggtgaaaaaa
     2161 ctgttgagca attcccacaa tggttccagg aacaaggata taaggatatc ccagttcttt
     2221 atgataccaa agcaaccacc ttccaagctt atcaaattcg aagcattcct acagaatatt
     2281 taattgatag ccaaggaaag attggaaaga ttcaatttgg tgctatcagt aatgcggatg
     2341 cagaagcagc atttaaagaa atgaactaga atcgataaaa tctgattaca agatgtagtc
     2401 agattttttt agaaaaacat tttataaata ttcacatatc tcctatattt atggtaaaat
     2461 agaattatca gtttattttg gagtcaaaga tgaatatatt tagaacaaag aatgttagtt
     2521 tagataaaac agagatgcat aggcatttga agttatggga tttgattttg ctgggtatcg
     2581 gagccatggt agggacaggc gtctttacaa tcacaggtac tgcagctgca acacttgctg
     2641 gcccagccct agtgatttca atcgttattt ctgccttgtg tgtgggatta tcagccctct
     2701 tttttgcaga atttgcctcg cgagtacccg ctacaggagg tgcctatagt tacctctatg
     2761 ctatcttagg agaattccct tcctggttgg ctggttggtt aaccatgatg gagttcatga
     2821 cagccatatc aggcgtagct tcgggttggg cagcttattt taaaggcttg ctctctcaat
     2881 acgggatagc ctttcctcag gctttaaatg gtacctttaa tcctcaagca gggacatttg
     2941 ttgatttatt gcctattctt gtcttggtct tggtgacttc gcttgtttta ctaaatgcca
     3001 aggcagcctt acgctttaat tcgattctag ttattttgaa attttccgct ttagctctct
     3061 ttgttttagt aggaatttgg aatattaagt ttgataattg gagcaatttt gctccttacg
     3121 gttttggaca aatctatgga gctagcactg gtattatggc tggtgcgtcc ttgatgttct
     3181 tcggtttttt gggatttgaa tccatctcta tggctgtaga tgaagtcaag actcctcaaa
     3241 aaaatattcc tagagggatt gtcttatcgc tttctatcgt aaccattctc tatgccttgg
     3301 tgacccttgt tttgactggt gtggttcact atagtcattt aaatgtcgat gatgccgttg
     3361 cctttgccct tcgtagtgtt gggattagtt gggcagccaa ctatgtgtca ttagtggcta
     3421 tcttgacctt gattacagtt tgtatttcga tgacctatgc cctatcgcgt atgatttaca
     3481 gtttagcacg tgacggctta gtgcctgctg cctttaagga actgacgaag actagcaaga
     3541 taccaaagaa tgctactatc ttgacaggtc tagcttcagc agtagcagca ggaatgttcc
     3601 cactagccag tattgcagcc tttttaaata tttgtacctt agcctacttg atcatgctgg
     3661 cttatggtct gattcgctta cggaaagaaa aaggaatgcc caaagctggg gaatttaaaa
     3721 caccattggt acctctgtta ccaattttat cgatcatcat ttgtttatcc ttcatgttac
     3781 agtataatat gaacacctgg attgctttcc tagttgcgtt gttagtagga agtattattt
     3841 acttcactta tggctataag cattctacca tagaagaata aagtgaaagt ctattgagtt
     3901 gttgacactc agtagatttt tatatgtgaa agaaatttca aattttttct aaaaatatct
     3961 tgacagatta aattttcagg agtagaatat ttactagtta attaaatggt taaggagttg
     4021 ttcatgaaga aacaaaattt atttttagtc ctgttaagtg tctttctttt gtgtttgggg
     4081 gcttgtggtc aaaaggaaag tcagacagga aaggggatga aaattgtgac cagtttttat
     4141 cctatctacg ctatggttaa ggaagtatct ggtgacttga atgatgttcg gatgattcag
     4201 tcaagtagtg ggattcactc ctttgaacct tcggcaaatg atatcgcagc catctatgat
     4261 gcagatgtct ttgtttacca ttctcataca ctcgaatctt gggcaggaag tctggatcca
     4321 aatctaaaaa aatccaaagt gaaggtctta gaggcttctg agggaatgac cttggaacgt
     4381 gtccctggac tagaggatgt ggaagcaggg gatggagttg atgaaaaaac gctctatgac
     4441 cctcacacat ggctagatcc tgaaaaagct ggagaagaag cccaaattat cgctgataaa
     4501 ctttcagagg tggatagtga gcataaagag acttatcaaa aaaatgcgca agcctttatc
     4561 aaaaaagctc aggaattgac taagaaattc caaccaaaat ttgaaaaagc gactcagaaa
     4621 acatttgtaa cacaacatac agccttttct tatctagcga agagatttgg gcttaatcaa
     4681 cttggtattg caggtatctc tcctgaacaa gaaccaagtc cacgacaact aacagaaatt
     4741 caggaatttg ttaagaccta taaggttaaa acgattttta cagaaagtaa cgcttcttca
     4801 aaagtagctg aaactcttgt caaatcaaca ggtgtgggtc ttaaaactct gaatccttta
     4861 gagtcagacc cacaaaatga caagacctat ttagaaaatc ttgaagaaaa tatgagtatt
     4921 ctagcagaag aattaaagtg aggaaagaat gaaaatcaat aaaaaatatc tagcaggttc
     4981 agtggcagtc cttgccctaa gtgtttgttc ctatgaactt ggtcgtcacc aagctggtca
     5041 ggttaagaaa gagtctaatc gagtttctta tatagatggt gatcaggctg gtcaaaaggc
     5101 agaaaatttg acaccagatg aagtcagtaa gagagagggg atcaacgccg aacaaattgt
     5161 tatcaagatt acggatcaag gttatgtgac ctctcatgga gaccattatc attactataa
     5221 tggcaaggtt ccttatgatg ccatcatcag tgaagaactt ctcatgaaag atccgaatta
     5281 tcagttgaag gattcagaca ttgtcaatga aatcaagggt ggctatgtga ttaaggtaga
     5341 cggaaaatac tatgtttacc ttaaagatgc agcccatgcg gacaatattc ggacaaaaga
     5401 agagattaaa cgtcagaagc aggaacgcag tcataatcac aactcaagag cagataatgc
     5461 tgttgctgca gccagagccc aaggacgtta tacaacggat gatgggtata tcttcaatgc
     5521 atctgatatc attgaggaca cgggtgatgc ttatattgtc cctcacggca atcacttcca
     5581 ctatattcct aagagtgatt tgtctgccag tgaattagct gctgcccaag cctattggaa
     5641 tgggaagcag ggatctcgtc cttcttcaag ttctagtcat aatgcaaatc cagctcaacc
     5701 aagattgtca gagaaccaca atctgactgt cactccaact tatcatcaaa atcaagggga
     5761 aaacatttca agccttttac gtgaattgta tgctaaaccc ttatcagaac gccatgtaga
     5821 atctgatggc ctagtatttg atccggcaca aataacatat cgtacagtca atggtgttgc
     5881 tgtaccgcac ggagaccatt atcactttat tccttattca caactgtcac ctttggaaga
     5941 aaaattggct cgtatgattg ctgttaaggg acaaaatggt gcagtcttgc caggaatgca
     6001 ttatttgaaa ccagcaccaa aaccgcaagt tcaaccatca actgagaaaa aacaaactga
     6061 ttttgcagta gaacaggttg ttagaaaagt tggagaaggc tatgttgtgg aaattgctgg
     6121 tgtcagtcac tatgtttttg ctaaagactt ggctaaggat aagattgatg ctattgaaaa
     6181 tctcttgtct aagaaaactc aagagacaca tgctctagtt gctaaaaaag aaaatgtcgc
     6241 tcctcgtgac caagaatttt atgataaagc ttacaatctg ttaacccagg ctcataaagt
     6301 cttgtctgaa aataaaggtc gtacttctga tttccaagcc ttagacaaat tagcagaacg
     6361 cttgaataat gaatcttcta ataaagtaaa attggtagat gatttattgg cattcctagc
     6421 accaattacc cacccagaac gccttgg
//
LOCUS       DAVBCB010000086         4871 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVBCB010000086 DAVBCB010000000
VERSION     DAVBCB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.086"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            191..409
                     /locus_tag="VQU28_002009"
     CDS             191..409
                     /locus_tag="VQU28_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HET2477301.1"
                     /translation="MKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVS
                     AIDSSEAMLLPTINVLELLDKSQPFEEE"
     gene            1454..2446
                     /locus_tag="VQU28_002010"
     CDS             1454..2446
                     /locus_tag="VQU28_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2477302.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            2448..3266
                     /locus_tag="VQU28_002011"
     CDS             2448..3266
                     /locus_tag="VQU28_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HET2477303.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVIYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            3268..4053
                     /locus_tag="VQU28_002012"
     CDS             3268..4053
                     /locus_tag="VQU28_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2477304.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            4092..4370
                     /locus_tag="VQU28_002013"
     CDS             4092..4370
                     /locus_tag="VQU28_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HET2477305.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            4355..4552
                     /locus_tag="VQU28_002014"
     CDS             4355..4552
                     /locus_tag="VQU28_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2477306.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFPQLWNPQNPCQILIKKLE"
BASE COUNT         1399 a          796 c         1022 g         1654 t
ORIGIN      
        1 ggctagtttc ctagtttgct ctttggtttt catttagtat aacagattgt tagaagttgg
       61 tttaaatttc ctaatcagtt tgttcacatt taccttcgat atattatatc ccatagttaa
      121 ggttggtcat acagatgatt atagtcatgg agccgtaaaa cttagtgttt ctttagttga
      181 caaagatgcc atgaaaaaaa tatctgtaac tgtaatagga tattttgaaa taaatataga
      241 tgaaaatatc accgatattc tatacgtaaa tggtactgct attctttatc attatttacg
      301 ttctattgtt tcaatagttt cggcaattga tagcagtgaa gcaatgttgc tacctaccat
      361 taatgtttta gagttactag ataaatctca accttttgaa gaagaataat ttattagctc
      421 actaaattga gggtaaggaa aagtaaaatc agtaagaaaa atgtcttgca ttatacagca
      481 accttttggg aatgagtaga tggattgaat aaaattttat taagagtgga tgatttatct
      541 gtagattatt attggacagt tagtcttgaa gtagtctaag aattaggtta taatcagtag
      601 aatccttgct aataatgagg aggttagttt atgtatagta gactgaatct aaaatagtac
      661 gaaacaattg ctaaaacatt tatagaaatt aattttactt tcccaatcga tttgttctca
      721 tcttatttca atccgctata tattatggta tcgaatcttc atcagaatga taaaattaat
      781 caattgatat ctgattacaa acagaatatg aaagcttttt atatcactat tgaaaaattt
      841 atacgagatg atgaaagcct taagtgttat tttataaagg ttatttcaag tcgttccaag
      901 gtaacaagtc tagatcagat tgaagctgat aaaacgatac aaagaaaata ttcaagtgag
      961 ctaaaaaaat ttattggatt ttataatgag attatttgtg aggaaaatag tttcctacat
     1021 gtacgaaaga ggtggtcgag ttggtttagg tagtcgatgc gtgagttgat aattctcagg
     1081 gtatggactt ctttttcatg aatgaggtaa aagagcaggt attgtttaga gacaatcatt
     1141 ctgagcatat tttctggata gagggagtat ccgattttat gatcaaagtt aataccgccc
     1201 tctggtgaga agatgagtag gttggtaatt taaactatta aacagaattt ttgattaaaa
     1261 gtattatttt atgagagaaa tcctaatttc acaatccata ggcaaactct tgcatttcgt
     1321 tttttattgg actataatag gttggtataa agccttttgt agtaataaaa tgtagaaggt
     1381 gtagaaagca aggatttaga atatttgtag ttaaaaacac aatgttgcta ttccttacga
     1441 tagggagata gatatggcaa tgatagaagt ggaacatctt cagaaaaatt ttgtgaagac
     1501 tgttaaggaa ccgggcttga agggggcttt gcgctccttt attcatcctg aaaagcagac
     1561 ctttgaagcg gtcaaggatt tgacctttga ggttccaaaa gggcagattt taggatttat
     1621 cggggcaaat ggtgctggga agtcgacaac cattaaaatg ctgacaggaa ttttgaaacc
     1681 aacatctggt ttttgtcgga ttaacggcaa gattccccag gacaatcggc aagattatgt
     1741 caaagatatt ggcgtagtct ttggacaacg cacccagcta tggtgggatt tggctctgca
     1801 agagacctac actgtcttaa aagagattta tgatgtgcca gactcgctct ttcataagcg
     1861 tatggacttt ttgaatgaag tcttggattt gaaggacttt atcaaggatc ccgtgcggac
     1921 tctttcactg ggacaacgga tgcgggcgga tattgcggcc tccttgctcc acaatcccaa
     1981 ggttcttttt ttagatgagc cgaccattgg tttggacgtt tcggttaagg ataatattcg
     2041 tcgggcaatt actcagatca atcaagagga agaaactacc attcttttga ccactcacga
     2101 tttgagtgat attgagcaac tttgtgatcg gattttcatg attgacaagg ggcaagagat
     2161 ttttgatgga acggtgagcc aactcaagga gacctttggt aagatgaaga ctctctcttt
     2221 tgaactgcta ccaggtcaaa gtcatctcgt ctctcactat gacggtctgt ctgatatgac
     2281 cattgataga caaggaaaca gcctcaacat tgaatttgat agttctcgct accagtcagc
     2341 tgacattatc aagcaaaccc tgtctgattt tgaaatccgc gatttgaaga tggtggatac
     2401 ggatattgag gatattatcc gtcgcttcta ccgaaaggag ctctaggatg atcaaattgt
     2461 ggagacgtta taaacccttt atcaatgcag gggttcagga gttgattact taccgagtca
     2521 actttattct ctatcggatt ggcgatgtca tgggggcttt tgtggccttt tatctctgga
     2581 aggctgtctt tgattcttcg caagagtctt tgattcaggg cttcagtatg gcggatatca
     2641 ccctctacat catcatgagt tttgtgacca atcttctgac tagatccgat tcgtccttta
     2701 tgattgggga ggaggtcaag gatggctcca ttatcatgcg tttgttgcga ccagtgcatt
     2761 ttgcggcctc ctatcttttc accgagcttg gttccaagtg gttgattttt atcagcgttg
     2821 gccttccatt tttaagtgtc attgtcttga tgaaaatcat atcgggtcaa ggtattgtag
     2881 aggtgctagg attaactgtc atttatcttt ttagcttaac gctcgcctat ctgattaact
     2941 ttttctttaa tatttgcttt ggattttcag cctttgtgtt taaaaatctt tggggttcca
     3001 acctacttaa gacttccata gtggctttta tgtcggggag tttgattccc ttggcattct
     3061 ttccaaaggt tgtttcagat attctctcct ttttgccttt ttcatccttg atttatactc
     3121 cagttatgat cattgttgga aaatacgatg ccagtcagat tcttcaggca ctccttttgc
     3181 agttcttctg gctcttagtg atggtgggat tgtctcagtt gatttggaaa cgggtccagt
     3241 cctttatcac cattcaagga ggttagtatg aaaaaatatc aacgaatgca tctgattttt
     3301 atcagacaat acatcaaaca aatcatggaa tataaggtgg attttgtggt tggtgtcttg
     3361 ggagtctttc tggctcaagg cttgaatctc ttgtttctca atgtcatctt tcaacatatt
     3421 ccattcctag aaggctggac ctttcaagag atagctttca tttatggatt ttccttgatt
     3481 cccaagggaa tggaccatct cttttttgac aatctctggg cactagggca acgcctagtc
     3541 cgaaaagggg agtttgacaa gtatctgact cgtcccatca atcctctctt tcacatccta
     3601 gttgaaacct ttcagattga tgccttgggt gaactcttag tcggtggtat tttattggga
     3661 acaacagtga ccagcattgt ttggactctt ccgaaattcc tgcttttcct agtttgtatt
     3721 ccttttgcga ccttgattta tacttctctt aaaatcgcaa cagccagtat cgccttttgg
     3781 actaagcagt caggcgccat gatttacatc ttctatatgt tcaatgactt tgctaagtat
     3841 ccgatttcta tttacaattc tcttcttcgt tggttgatta gctttatcgt gcctttcgcc
     3901 tttacagcct actatccagc tagctatttc ttacaggaaa aggatgtgtt ctttaacgta
     3961 ggaggtttga tgttgatttc tctggttttc tttgttattt cccttaaact ttgggataag
     4021 ggcttagatt cctacgaaag tgcgggttcg taaaagctaa agtaagacta aaatcaagaa
     4081 agaaacttat gatgtttgta attgaagaag tcaaggatga aaatcaaaaa aaggcagttg
     4141 tagctgaggt tttgaaggat ttgccagaat ggtttggaat cccagaaagc acacaagcct
     4201 atatagaagg aaccacgaca ctgcaagttt ggaccgccta tcaggagagt gatttgacta
     4261 gatttgtaag cttatcctat tcgagtgaag attgtgcaga gattgattgt ctcggcgtaa
     4321 aaaagcttat caaggtagaa aaattgggag ccaattgctt gctactttag agagtgaagc
     4381 tcgtaaaaaa gttggttatc tgcaggtcaa aacagtggca gaaggttcta ataaagatta
     4441 tgatcgaaca aatgactttt atcgaggtct tggctttaaa aagttagaga tttttcctca
     4501 actatggaat ccgcaaaatc cttgtcagat tttgattaaa aagcttgaat aatattactt
     4561 gacatctatt ctcagagtgc catactgtaa gtgtaatcgc cgatttagct cagttggtag
     4621 agcaaggcac tcgtaaagcc taggttatag gtagataaac gactgaggag ttgaaaaaat
     4681 agataggtag aagataaccg ttaagcctta ttcttagcgg ttatttatat tgtttaatag
     4741 cgctgatatt tcatcaatta tgcctgtttt cgtgtttctg gtagttgttc aagtttattg
     4801 ctactatttt tgatggtatg aatgtgctta taatgtatcc cggttaacga aagttttgga
     4861 cttatactct t
//
LOCUS       DAVBCB010000087         4552 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVBCB010000087 DAVBCB010000000
VERSION     DAVBCB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.087"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(112..549)
                     /locus_tag="VQU28_002015"
     CDS             complement(112..549)
                     /locus_tag="VQU28_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HET2477307.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQU28_002016"
     CDS             complement(634..3267)
                     /gene="polA"
                     /locus_tag="VQU28_002016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HET2477308.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            3520..3873
                     /locus_tag="VQU28_002017"
     CDS             3520..3873
                     /locus_tag="VQU28_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HET2477309.1"
                     /translation="MILRHLGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAKNVTIL
                     EGSNIHVLPSMPYSAQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            4088..4204
                     /locus_tag="VQU28_002018"
     CDS             4088..4204
                     /locus_tag="VQU28_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HET2477310.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            4197..4430
                     /locus_tag="VQU28_002019"
     CDS             4197..4430
                     /locus_tag="VQU28_002019"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477311.1"
                     /translation="MFSQLHMREEQVLLAQDYALETARAEGLEQGLERGKVEGRAERK
                     LFAFLDIVRQGLLTSEVASQQLGMSVSEFEALL"
BASE COUNT         1385 a         1096 c          837 g         1234 t
ORIGIN      
        1 gtatctacaa cctcaaaaca gtgttttgag cagcccgcga ctagtttcct agtttgctct
       61 ttgattttca ttgagtataa cacaaaaggt agcccatcag ctaccttttt cttatgcttc
      121 ctcaatcaag cgagtatgtt ctctcttgat acagcgattc atcacgatat catcacatcc
      181 accatcacgc aaaatctctt tcgcttctaa actttcaagt cctagctgtg cccaaaaaat
      241 cttggcatca gctttgagaa aatcacgcgc cacatcgggc agaaattcac tgcgacgata
      301 aacattgaca atatctacag gaaaaggaat ttcagcgagg ctagcataag ccttttcacc
      361 caagatttcg ccacctgccg ccttgggatt gactgggatg attttatagc cccgagcctg
      421 catttccttt gtcactcgat tgctggttgt ttcttcacgg tcagacaaac ccaccacagc
      481 aagggtttta ctcgttgcga gatactgacg aatcacgcca tcacttggat tgataaattc
      541 ttgactcata gaaaacctcc tttttcatca gtatagcaca ttttgaaaag gtttgcagaa
      601 ttctactaca aaaaaggagg actagccccc tttttattta gcctcgtacc aggttgcccc
      661 ttcattctca tctgcgataa gaggaacact gagttgaatg gcttcttcca tggtttgttt
      721 caccaatttt ttcatctcta ccaattcaga tttaggcact tcaaggacga tttcatcgtg
      781 cacttgtaac agcatcttag tctgataacc acctgcaacc aaggctttat ccagctgaat
      841 catggcaatc ttgagaatat ctgctgccga accctggata ggtgagttga tagcagttcg
      901 ctccgcaaaa ccacgaatat tgaagttgcg cgaattgata tctggcaact cacggcgacg
      961 cttaaagagg gtctctacat agcccttatc acgcgcctcc cgcaccactt catccatgta
     1021 gtttttaata cctggaaaac gttcaaagta ggtatcaatg taggctttgg cttccttacg
     1081 actaattccc aaattattag acaagccaaa gtctgaaatc ccataaacca ctccaaagtt
     1141 aactgccttg gcattgcgac gatcatttgc agtcacatta tcaggacgct caatgccaaa
     1201 gacccgcatg gctgtcgaag tatggatatc tgccccctct tggaaggcct taatcaagtg
     1261 ctcatcctta gaaatatgcg ccaaaacgcg caattcaatc tgtgaatagt cagagctgag
     1321 tagcacacta tcctcccact ctggcacaaa agccttccga atcaagcgcc cttgttccaa
     1381 tcgggcagga atattttgca agtttggatc cacactagac aaacgcccgg tctgggtcaa
     1441 atcctgcata tagcgagtat gaatctttcc atcagccaaa atccagtcct gcaagccaat
     1501 tacataagta gattgaatct tagcaatttg acggtaatcc aggattttct taacaatcgg
     1561 agcaatagga gcgagacgct ccaacacatc cactgctgtc gaataacctg tcttggtttt
     1621 cttagtgtat tctagaggaa gtcccaattt ctcaaagaga agcacgccca actgcttagg
     1681 cgagttgaca ttaaactcct caccagccag ctcgtaaatc tcttgagtca gtttttcaat
     1741 gacaagctca ttttcagcct gcatctcaag caaggtctct ttcttgacca caatcccagc
     1801 aatttccatc ttggcaagaa caaaagccag aggttgctcc atatcataaa gaagctctaa
     1861 ttgcccattt tcgctgagtt tttcaagtaa aataggctct gtttctacca aaacagcaag
     1921 tttacaagct aagtgttcca agaatttctc acgttcagga atggcctttt taacaccctt
     1981 accgtagaaa gtttcatcat caaccaagta agtctgacca taaagactag cgatggtcgc
     2041 aatttcattg tcctccacag tcgaaaggag gtatttagcc aaacggatgt caaaagcagg
     2101 cgcctgcaaa tccacaccaa aacgttgcaa aagaacttta accttcttaa agtcataaac
     2161 tctcagagat gttttttcta agaaatcctt gaaaatcggg tcttgcaaca gctcaagctt
     2221 gtctgtggca tagagcttat ccccacaaga ccaggcaaat ccaaccaaat tatccgtatg
     2281 ataattctca ccaaaaagct caaagtggaa gatagactct tcactcagca tatcttgact
     2341 gatttggtca acaatagtaa aatccaaacc ctcagccaca tcagctgacg acacatttaa
     2401 agcctgcttg agctgtttga atcccatctc atcgtaaaat ttcccaagat tttcaacatc
     2461 tggaccacta tagaccaagt cctctaaacc aatcgcaatc ggtgccttgg tatcaatggt
     2521 cgctagtgtt ttagacaaaa aggcctgttc cttgtcattg atgagatttt ccttcatctt
     2581 agaagtcttc attccatcaa tattttcata aatcccctca agcgaaccat gctccagcaa
     2641 gagcttaata cccgtctttt caccgacttt ggtcacccca gggatattat ccgacttatc
     2701 acccatgagc gccttgagat cgataaactg agctggtgtg aggcccattt cttccatgag
     2761 gtaatctggc gtaaaggcct caaactcagc cacacctttc ttggaaattt caaccaccgt
     2821 atgctcatcc gtcagctgaa tcaaatcctt gtccccactg acaatggtaa tatcaaaacc
     2881 atcctgctct gctagcttat ccaacgtccc aatgatgtca tccgcctcat actgagccag
     2941 ctcatagtga cgaatcccca tatgatccag caactcacga atgaaaggaa attgctcacg
     3001 aaactcatca ggagtcttgg cccgaccacc cttatagtcc gcatacatct ctgtccggaa
     3061 ggtcgtcttt cccgcatcaa aagccaccaa aatatgactc ggctcaaccc gctccaacaa
     3121 atgactcaac atcaactgaa aaccataaat cgcattggta tgcaaaccag ccgcattctt
     3181 aaaacggtcc aactgctgat acagcgcaaa aaacgcccga aaagctacag aagacccatc
     3241 aatcaataat aattttttct tatccataca cccattataa aggaaagaat caaaaaatac
     3301 cattgggaag agctagagca agtatttttc aaactttttc cgaataaata gatagagcca
     3361 gagaatttag taaacctaga tttaaaaatg tgctataata tagtatattg aatctataat
     3421 agtacacctt gactgctaaa atatttctat aaattaattt gactttcctg atagagttgt
     3481 tcacatctta tttcaattca ctatagaagg aggaaaagga tgattctcag acatctggga
     3541 attagcccga ccaatgactt ggttgctaag aaaattttca gcaacccaga aatcacttgt
     3601 caatttatcc gcgatatgct ggacttgcca gccaaaaatg tgactatttt ggagggaagt
     3661 aacattcatg tcttgccttc catgccctac tcagcgcagg atttttatac cagtatagac
     3721 gtcttggcgg agttggataa cggtactcaa gtaattattg agattcaagt ccatcatcag
     3781 aattttttca tcaatcactt gtgggcttac ctgtgcagtc aggttaatca aaatcttgaa
     3841 aaaattcgtc agcgagaagg tgatactcac tagatctaca aacacatcgc tcctgtttac
     3901 gtcattgcta tcgtggatag taattatttc tcagatgacc tggcttttca tagctttagt
     3961 atgcgcgaag acacaacagg tgaggtattg gcgattacca acaatggaca ggaaaaccat
     4021 ctggttaaga tggcattctt ggaattaaaa aatacagaga aaccagcaaa gacaaggttc
     4081 gcaagccatg gttggagttt ttcggcaaca agccctttac ccagcaaccg caacgagcca
     4141 ttacccaagc aaatcaactg ctggactaca agagctggtc cgaggaggac aggaaaatgt
     4201 ttagtcaact acatatgcga gaagaacaag tcttgttagc acaggactat gccttggaaa
     4261 ctgctagggc tgaaggcctt gaacaaggac tagagcgtgg gaaagttgaa ggaagggcag
     4321 aaaggaaact ttttgccttc ctagacatag tacgccaagg tcttctgact tctgaggttg
     4381 ccagccagca attaggtatg tcagtatctg aatttgaggc actgttgtaa aatggctcca
     4441 taatatccat agtgggtaaa tcccctatgg atattatgga gcctattttt gtgtagaaaa
     4501 aaagtcccat atgacctata atgaaaagcg acaaaacaac tcattaaaaa ga
//
LOCUS       DAVBCB010000088         4483 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVBCB010000088 DAVBCB010000000
VERSION     DAVBCB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4483
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.088"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1261
                     /locus_tag="VQU28_002020"
     rRNA            <1..1261
                     /locus_tag="VQU28_002020"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1313..1385
                     /locus_tag="VQU28_002021"
     tRNA            1313..1385
                     /locus_tag="VQU28_002021"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1346..1348,aa:Ala,seq:tgc)
     gene            1510..4411
                     /locus_tag="VQU28_002022"
     rRNA            1510..4411
                     /locus_tag="VQU28_002022"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1240 a          920 c         1333 g          990 t
ORIGIN      
        1 atacatagcc gacctgagag ggtgatcggc cacactggga ctgagacacg gcccagactc
       61 ctacgggagg cagcagtagg gaatcttcgg caatggacgg aagtctgacc gagcaacgcc
      121 gcgtgagtga agaaggtttt cggatcgtaa agctctgttg taagagaaga acgagtgtga
      181 gagtggaaag ttcacactgt gacggtatct taccagaaag ggacggctaa ctacgtgcca
      241 gcagccgcgg taatacgtag gtcccgagcg ttgtccggat ttattgggcg taaagcgagc
      301 gcaggcggtt agataagtct gaagttaaag gctgtggctt aaccatagta ggctttggaa
      361 actgtttaac ttgagtgcaa gaggggagag tggaattcca tgtgtagcgg tgaaatgcgt
      421 agatatatgg aggaacaccg gtggcgaaag cggctctctg gcttgtaact gacgctgagg
      481 ctcgaaagcg tggggagcaa acaggattag ataccctggt agtccacgct gtaaacgatg
      541 agtgctaggt gttagaccct ttccggggtt tagtgccgta gctaacgcat taagcactcc
      601 gcctggggag tacgaccgca aggttgaaac tcaaaggaat tgacgggggc ccgcacaagc
      661 ggtggagcat gtggtttaat tcgaagcaac gcgaagaacc ttaccaggtc ttgacatccc
      721 tctgaccgct ctagagatag agttttcctt cgggacagag gtgacaggtg gtgcatggtt
      781 gtcgtcagct cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca acccctattg
      841 ttagttgcca tcatttagtt gggcactcta gcgagactgc cggtaataaa ccggaggaag
      901 gtggggatga cgtcaaatca tcatgcccct tatgacctgg gctacacacg tgctacaatg
      961 gctggtacaa cgagtcgcaa gccggtgacg gcaagctaat ctcttaaagc cagtctcagt
     1021 tcggattgta ggctgcaact cgcctacatg aagtcggaat cgctagtaat cgcggatcag
     1081 cacgccgcgg tgaatacgtt cccgggcctt gtacacaccg cccgtcacac cacgagagtt
     1141 tgtaacaccc gaagtcggtg aggtaaccgt aaggagccag ccgcctaagg tgggatagat
     1201 gattggggtg aagtcgtaac aaggtagccg tatcggaagg tgcggctgga tcacctcctt
     1261 tctaaggata aggaactgcg cattggtctt gtttagtctt gagaggtctt gtggggcctt
     1321 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc agcggttcga tcccgctagg
     1381 ctccattggt gagagatcac caagtaatgc acattgaaaa ttgaatatct atatcaaata
     1441 gtaacaagaa aataaaccga aaacgctgta gtattaataa gagtttatga ctgaaaggtc
     1501 agaaaataag gttaagttaa taagggcgca cggtggatgc cttggcacta ggagccgacg
     1561 aaggacgtga caaacgacga tatgccttgg gtagctgtaa gtaagcgatg atccagggat
     1621 ttccgaatgg gggaacccaa caggtagtac ctgttaccca catctgttaa ggatgtgagg
     1681 aggaagacgc agtgaactga aacatctaag tagctgcagg aagagaaagc aaaagcgatt
     1741 gccttagtag cggcgagcga aacggcagga gggcaaaccg aagagtttac tcttcggggt
     1801 tgtaggactg caatgtggac tcaaagatta tagaagaatg atttgggaag atcagccaaa
     1861 gagagtaata gcctcgtatt taaaatagtc tttgtaccta gcagtatcct gagtacggcg
     1921 ggacacgtga aatcccgtcg gaatctggga ggaccatctc ccaaccctaa atactcccta
     1981 gtgaccgata gtgaaccagt accgtgaggg aaaggtgaaa agcaccccgg gaggggagtg
     2041 aaatagaacc tgaaaccgtg tgcctacaac aagttcgagc ccgttaatgg gtgagagcgt
     2101 gccttttgta gaatgaaccg gcgagttacg ttatgatgcg aggttaagtt gaagagacgg
     2161 agccgtaggg aaaccgagtc tgaatagggc gccttagtat catgacgtag acccgaaacc
     2221 atgtgaccta cccatgagca ggttgaaggt gcggtaagac gcactggagg accgaaccag
     2281 ggcacgttga aaagtgcttg gatgacttgt gggtagcgga gaaattccaa acgaacttgg
     2341 agatagctgg ttctctccga aatagcttta gggctagcgt cgacattaga gattcttgga
     2401 ggtagagcac tgtttgggtg aggggtccat cccggattac caatctcaga taaactccga
     2461 atgccaatga attatggtcg gcagtcagac tgcgagtgct aagatccgta gtcgaaaggg
     2521 aaacagccca gaccaccagc taaggtccca aaataattgt taagtggaaa aggatgtggg
     2581 gttgcacaga caactaggat gttagcttag aagcagctat tcattcaaag agtgcgtaat
     2641 agctcactag tcgagtgacc ctgcgccgaa aatgtaccgg ggctaaaaca atttaccgaa
     2701 gctgtggata cctttatagg tatggtagga gagcgttcta tgtgtgatga aggtataccg
     2761 tgaggagtgc tggaacgcat agaagtgaga atgccggtat gagtagcgaa agacaggtga
     2821 gaatcctgtc caccgtaaga ctaaggtttc caggggaagg ctcgtccgcc ctgggttagt
     2881 cgggacctaa ggagagaccg aaaggtgtat ccgatggaca acaggttgat attcctgtac
     2941 tagagtatgt agtgatggag ggacgcagta ggctaactaa agcagacgat tggaagagtc
     3001 tgtctaagca gtgaggtgtg atatgagtca aatgcttata tctgtaacat tgagctgtga
     3061 tggggagcga agtttagtag cgaagttagt gacgtcacac tgccaagaaa agcttctagc
     3121 gtttaaacat actctacccg taccgcaaac cgacacaggt agtcgaggcg agtagcctca
     3181 ggtgagcgag agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa
     3241 ggggtgctga ctttaagtca gccgcagtga ataggcccaa gcaactgttt atcaaaaaca
     3301 cagctctctg ctaaatcgta agatgatgta tagggggtga cgcctgcccg gtgctggaag
     3361 gttaagagga gtgcttagcg taagcgaagg tatgaattga agccccagta aacggcggcc
     3421 gtaactataa cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acccgcacga
     3481 aaggcgtaat gatttgggca ctgtctcaac gagagactcg gtgaaatttt agtacctgtg
     3541 aagatgcagg ttacccgcga caggacggaa agaccccatg gagctttact gcagtttgat
     3601 attgagtgtc tgtaccacat gtacaggata ggtaggagtc taagagatcg ggacgccagt
     3661 ttcgaaggag acgctgttgg gatactaccc ttgtgttatg gccactctaa cccagatagg
     3721 tgatccctat cggagacagt gtctgacggg cagtttgact ggggcggtcg cctcctaaaa
     3781 ggtaacggag gcgcccaaag gttccctcag aatggttgga aatcattcgc agagtgtaaa
     3841 ggtataaggg agcttgactg cgagagctac aactcgagca gggacgaaag tcgggcttag
     3901 tgatccggtg gttccgtatg gaagggccat cgctcaacgg ataaaagcta ccctggggat
     3961 aacaggctta tctcccccaa gagttcacat cgacggggag gtttggcacc tcgatgtcgg
     4021 ctcgtcgcat cctggggctg tagtcggtcc caagggttgg gctgttcgcc cattaaagcg
     4081 gcacgcgagc tgggttcaga acgtcgtgag acagttcggt ccctatccgt cgcgggcgta
     4141 ggaaatttga gaggatctgc tcctagtacg agaggaccag agtggactta ccgctggtgt
     4201 accagttgtc ttgccaaagg catcgctggg tagctatgta gggaagggat aaacgctgaa
     4261 agcatctaag tgtgaaaccc acctcaagat gagatttccc atgattatat atcagtaaga
     4321 gccctgagag atgatcaggt agataggtta gaggtggaag tgtggcgaca catgtagcgg
     4381 actaatacta atagctcgag gacttatcca aagtaactga gaatatgaaa gcgaacggtt
     4441 ttcttgaatt gaatagatat tcgattttga gtaggtatta ctc
//
LOCUS       DAVBCB010000089         4438 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVBCB010000089 DAVBCB010000000
VERSION     DAVBCB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.089"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..119
                     /locus_tag="VQU28_002023"
     CDS             <1..119
                     /locus_tag="VQU28_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HET2477312.1"
                     /translation="QQGFTDRLVGYSSSIALVFTTLIGMISLVQRRVLKEDD"
     gene            121..966
                     /locus_tag="VQU28_002024"
     CDS             121..966
                     /locus_tag="VQU28_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HET2477313.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            976..2295
                     /locus_tag="VQU28_002025"
     CDS             976..2295
                     /locus_tag="VQU28_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HET2477314.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEETGVRQVQNMAFSDDGIHFEKISQNPVATGSDLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWREKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(2503..2575)
                     /locus_tag="VQU28_002026"
     tRNA            complement(2503..2575)
                     /locus_tag="VQU28_002026"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2540..2542),aa:Lys,seq:ctt)
     gene            complement(2839..4110)
                     /locus_tag="VQU28_002027"
     CDS             complement(2839..4110)
                     /locus_tag="VQU28_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HET2477315.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYAARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQRIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
BASE COUNT         1294 a          809 c          976 g         1359 t
ORIGIN      
        1 atcaacaagg ttttactgat cgtcttgtag gatattctag ctcaatcgct ttagtattta
       61 caacgttaat tggtatgatt agcttagtac aacgtcgcgt tctgaaggag gatgactaag
      121 atgaaacgat ttaaaccaac aacaatctta gaatatattc tgttaacttt attggcagga
      181 ctcttccttt ttcctatggt ttggatggta gtttcagcta tgaagccaga agcggatgtt
      241 tataaaaatt tgactagttg gcaagccttc ttaccaagtt taaatcccgc tgattggttt
      301 aaaccttatc aggaagtatt tgagcgcttt agtattttta catatctagg gaacagcatt
      361 ttttatgctg gaacgtttgc tattggttct attattgtca atgcactggc cggtttcgct
      421 tttgcaaaag tcaatttttc aggtaagaaa attttatttg gtttcttact tgctttactt
      481 attattccag tggaaactgt gttaattcca caatttacca ttatcaattc cttaggtttg
      541 gtcaataatc gtcttgcagt tattattcca ggattggcga gtgtatttaa tatttactta
      601 tttaggaatt tctttattgc aattccagaa gagattcttg agtctgcaaa gatggacgga
      661 gcaagtatcg ttagaatttt ctttagaatt atgcttccaa tgtctaaacc tgcagttgct
      721 acagttggtg ttttatcatt tatttcaagc tggaatgatt atatctggcc attgatggtt
      781 ttgacagatt cttctaaatt ttcaatgcag gttgcaatta caactattaa tacaactcaa
      841 ccagtttata tcaatcaggt aatggctgta ttaactattt caacgattcc gttgattttg
      901 gtctatgtag tcgctcagaa atatattgtt caaggtctgg gtggttccgg aactggaata
      961 aaataggata tacttatgat aaataaaaca tttacagtag aaaaagccaa tcgttttata
     1021 gcagaaaata aacatctcgt taatactcaa tataagcctg aagaacattt ttcagctgag
     1081 attggttgga tcaatgatcc aaatggattt gtctattttc gtggagaata ccatctcttt
     1141 tatcaattct atccatatga tagtgtttgg gggcctatgc actggggaca tgctaaaagt
     1201 aaggacttgg tgacttggga gcacttgcca gtggcacttg ctcctgacca agattatgac
     1261 cgaaatggtt gtttctcagg ctctgccatt gtcaaggatg atcgcctctg gctcatgtac
     1321 actggacata tcgaagaaga aaccggtgtc cgccaagtgc aaaatatggc attttcagat
     1381 gacgggattc actttgaaaa gatttcccaa aatccagttg caactggatc agacttgcca
     1441 gatgagttga ttgctgctga tttccgtgat ccaaaactct ttgaaaaaga tggacgctat
     1501 tactccgtag tagctgccaa acacaaggat aatgtgggct gtatcgttct actagggtcc
     1561 gataacctag tagaatggca gttcgaatcc atctttttaa aaggaggaga acaccaaggc
     1621 tttatgtggg aatgcccaga ttacttcgag ttagatggga aagattgcct tattatgtca
     1681 cccatgcgtt atcagcgtga gggagactca tatcataaca tcaactcatc gcttttgttc
     1741 acgggtaagg tagattggag agaaaaacgt tttatcccag aatcagttca agaaattgat
     1801 catggccaag acttctatgc gcctcaaaca ttgttggacg atcaaaatcg tcgtatcctg
     1861 attgcttgga tgcagacatg ggggcgtacc cttccaaccc atgaccaaga acacaaatgg
     1921 gcatgtgcca tgactttacc tagaattcta agattggaag atggcaaact aagacaattc
     1981 cctgttaaaa aaggccaata tcaaatccaa atagataaag attgtcatta ccacttagga
     2041 aatgatacag attatcttga atttggttat gacagtaatg cgcagcaagt ttacattgat
     2101 cgtagccatc ttattcaaaa aattctaggt gaagaagaac aggacactag tcgacggtat
     2161 gtagatattg aagctaaaga attggaagtt gttctagata aaaattccat cgagattttt
     2221 gtcaatcaag gtgaagcaag cttgactgca acttattact taacggtgcc agctgagcta
     2281 tcacgaattg attaaaaatt aagttatttc tcctaaagaa aaagttctct ttctaaaata
     2341 gtggaaagag gactttttgt gttttgggta tataagctta gtttatggta tttgtaaaat
     2401 tggtgttgga ttatgattta agctagtttt ctaaagaatt tgaaaaaaat tttatttaag
     2461 caaaaaaacc ttggttccaa ggcttttcct gttgtattta gatgccccct acagggatcg
     2521 aacctgtgac ccacggatta agagtccgct gctctgccag ctgagctaag gaggcaaaga
     2581 aaaagctgta ttggtgccga aacttcacgg tttgtattga acccgcgcaa ttaagcaggt
     2641 gggcaactcg ctctaactga agctgtttcc gtgtgagacg gcctacatgc tgttagaaga
     2701 cttttgtttc cctaataata caaaaaatag tcggtcaaca cttaagtgtg aagtcgtaca
     2761 ccacagcgtt tctatactta tatgatacca ctttttcaaa aaaaatcaag aggaaagtgc
     2821 aattttttga aaaggatttc agatttttcg caaacgatcg atagcttcct ttctttgagc
     2881 caaagcccgt tcatatttac cagtatcttc tgcttggaaa tagtgatgat tacgaatttt
     2941 ttctggcaga tagtcttgct tgatccaatt tccaggatag ttgtgtggat agagatagtc
     3001 ttgggcattc cccagttcct tgcttccact gtagtgccca tcacgcaggt gtcgcggaat
     3061 aggcaagtgc cctgatgttt tgaggtcagc aagtgcctta tccatagcta cataggctga
     3121 gttggatttt ggagaaaggg ccaaatcaat cacgacattg gcaatgagaa tgcgggcttc
     3181 ggggaaacca atcctttgtg cagcatccag agcagtcacg gtatgaatct gggcctcagg
     3241 attggctaag ccaatatctt cataggcgat aacagtcaag cgacgagcga gactaggcag
     3301 atccccagcc tcaatcaagc gggcagcata gtggagactg gcatccacat ctgagccacg
     3361 gatagacttt tgcagggcag atagaacatc atagtgaccg tctccatcct tgtccatagt
     3421 gatgtagctt ctctgaagac tattttccat gatgtctaag gtgatgtggc gaatgccctc
     3481 gtcattctca ggggtagaga gaacagccaa gtccagtgag ttaaaggcag agcgaaggtc
     3541 tccgtttgta gaggttgcga tgaaatccag cgcatcctca tctagttcta ttggaaaatc
     3601 aaaaccacgt tcagggttac ttagagctat ctgcagggcc tctttgacgt cttggttaga
     3661 cagaggttcc aactcgaaaa tttgaacacg gctacgaatg gcaggagtga cagagaagaa
     3721 aggattttca gtcgttgctc caatcatgat gaccagtcca ctttccaaga gaggcaagag
     3781 gaagtcttgc ttggtcttat ctagtcgatg aatttcgtct agcaatagga cgagaccacc
     3841 agaaaattta gcttcttccg agatttcttg cagtcgcttt ttactatcaa ctgtcgcatt
     3901 gaaagttcga aaggcatact tggtcgttcc agcgatggca gaggcaatac tggttttgcc
     3961 gattccagga gggccatata gaatcatgga ggacaggcgg ttggcttcca ccatgcggcg
     4021 gatgattttt ccaggtccga ccagatgctc ctgaccgatg acctggtcga tggttttagg
     4081 gcgcatgcga agcgcgagat tgtctggcat agcagtcctt tctaacgtgg atttgagtag
     4141 aatgactcat ctactatatc ttactatgtc cattctagcc tattagttgt gatatttcaa
     4201 tggttttgta ataatcaggg agtgagatag aatctttttt actatttaat ttaatgagga
     4261 gaggtatcag catgagagat ttgattttcc ttaaagctca aaataggata gttgagccta
     4321 aagaagtgga agtgctgact tgagaaataa tattgctgaa tgtcattgta tgaactataa
     4381 tgaaaagaga ggtgataagc aatggaaatg atggaccaag tcaggaaatt ttgatttt
//
LOCUS       DAVBCB010000090         4296 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVBCB010000090 DAVBCB010000000
VERSION     DAVBCB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.090"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..141
                     /locus_tag="VQU28_002028"
     CDS             <1..141
                     /locus_tag="VQU28_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2477316.1"
                     /translation="LLPLPPYSPEYNPIEKTWAHIKKHLKKVLPSCNTFYEALLSCSC
                     FN"
     gene            complement(134..307)
                     /locus_tag="VQU28_002029"
     CDS             complement(134..307)
                     /locus_tag="VQU28_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HET2477317.1"
                     /translation="MNIKKRVLSAGLTFASVLLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(465..2072)
                     /locus_tag="VQU28_002030"
     CDS             complement(465..2072)
                     /locus_tag="VQU28_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HET2477318.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENPNSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTLAQEVFEKQILVPWDAFCVELL"
     gene            2225..3709
                     /locus_tag="VQU28_002031"
     CDS             2225..3709
                     /locus_tag="VQU28_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HET2477319.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGISPADRKVAVVARQKAEETGGPALALELPNGDIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3804..4286
                     /locus_tag="VQU28_002032"
     CDS             3804..4286
                     /locus_tag="VQU28_002032"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HET2477320.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1246 a          987 c          814 g         1249 t
ORIGIN      
        1 cttttacctc ttcctcccta ctcacctgag tacaatccta ttgagaaaac atgggctcat
       61 atcaaaaagc acctcaaaaa ggtattacca agttgcaata ccttttatga ggctcttttg
      121 tcttgttctt gtttcaattg actatattat cagtgtaata gtctaataga tagttaaatg
      181 tagttggatt tccactaaag gttgatgagt aagtttttgt atctgaacct gattggccgc
      241 aagcagctaa aagcaaaaca gatgcaaaag tcaggcctgc actaaggaca cgcttcttta
      301 tgttcatctt ctttctcctt aatagtggga atttgtaaag ttaattgaat ttcgagaatg
      361 aaagtttata aaccttggtt ataaaaaaca aaggatttct gcttttatac agtcctccct
      421 tgtttttata tgatttcaat tttaaatttt tctgcaaaaa atatttatag taattccaca
      481 cagaaagcat cccatggaac taagatttgt ttttcaaaga cttcttgagc tagggtgttt
      541 tcaatcaaga cagatttgac ttttccttct actgtcaagt cttgctcttc attggacaag
      601 ttagccacaa ctaggaagcg acggtcgcca tccttacgta tataagcaaa gaccttatca
      661 gccgtatcaa gcaattcaaa gtcagctcga actagccagc tattctcctt gcgaatttgg
      721 accagtttct gataggtata gaaaatagaa tctggatttg ccagcgcttc ttggacattg
      781 atcatctcgt aatttggatt aaccgccaac caaggttgac ctgttgagaa accagcgttt
      841 ttgctctcgt cccattgcat aggggtacgg gcattgtcac gtccaataac acggatactg
      901 tccatgattt cttcaatcgg aacacctttt tcaagagcct cacgcgcata gttgagagat
      961 tcaatatctt ctacttgatc cagtgtttca aacggatagt tggtcatccc aatctcctca
     1021 ccttggtaga tataaggagt tcctctcata agatgaagca agattgcaaa ggctttggca
     1081 gatttttcgc ggtattcttg gtcatttccc cagattgaga caatacgagg gaggtcatgg
     1141 ttgttccaga agagggaatt ccagccgtcc tcaactccta actctgtctg ccatttgttg
     1201 aagatttctt ttaacttagc gatattcagc tctttttgat agtgccattt aggctgacct
     1261 tcctgatact gaagaccgat atgttcaaac tggaagacca tagacaattc ttgccccttt
     1321 ggatcagagt agagcttggc aatctctggc gttgctcccc aagtctcccc tactgtcaag
     1381 agatccttat ctccaaaggt cgcctgattc atttccttga gatagggatg gagcatagga
     1441 ccattattga ctaccttctc gtcaggaatt ttgccaatca tgtcaataac atccatacgg
     1501 aaaccaccaa tacccttatc aatccagaag ttcatcatct cataaatttt ctggcgaagt
     1561 ttttcatttt cccagttgag atccggctgt ttcttgctga aaaagtggag atagtattga
     1621 cctgactttt catcgtattc ccaagcagac ccactaaaga tagaatctaa atcgttaggc
     1681 tcatcccgcc agatatagta gtctcgctca gggctattag gattttcaca ggcctctaca
     1741 aaccaggcat gctcatccga ggtatgattg accaccaagt ccatgatgat acggatatca
     1801 cgcttcttag cttctgcaat cagctgatcc atgtcctcca tggttccaaa aatagccgca
     1861 atcgcttgat aatcagcaat atcgtagcca ttatcatcca tagggctgtc ataaacggga
     1921 gaaagccaaa ttgctgtgat tcctagcttg gctagatagt ccaacttact ggtaattcct
     1981 ggcaaatcgc caattccatc tccattacta tccataaaac tctttggata gacttgatag
     2041 actacggcat tatgccacca tttttcttgc atctgtttac ctcattattt tcataatcta
     2101 tagtctatga tagcactttt taaaattttg tgcaagcgtt tgcttaatct ttttaatttc
     2161 ttttttagct ccgtagtttc ctaacttcca attttttatt ataatagagt ccagaggtaa
     2221 aaaaatgaaa aaacaagctt ttagttctga acagtatttg aatttacaac gcgaccatat
     2281 tttggagcgc attaaccaat ttgacggcaa gctctactta gagtttggcg gtaaaatgtt
     2341 agaagatttc cacgctgctc gtgtccttcc tggttatgaa cctgacaaca aaatcaagct
     2401 cttgcaagaa ttgaaagagc aggttgaggt tgtgattgcc attaatgcta gcaacattga
     2461 acattccaaa gcacgtggcg acttgggcat ttcttatgac caagaagttc ttcgtttgat
     2521 tgacaaattc aatgaattag ggatttttgt tggttccgtt gtcattacac aatacgctgg
     2581 ccaacccgct gcagatgcct tcctcaacca acttgagaaa aacggaattg attcttatct
     2641 tcattatcca atcaaaggat atccgacgga tatggatcac atcatttccc cagaaggcat
     2701 gggcaaaaac gactacatca aaaccagtcg caacttgatt gtcgtaaccg ctcctggacc
     2761 cggttctgga aaattggcaa cgtgtatgtc caatatgtac cacgaccaaa tcaatggcat
     2821 caagtctggc tacgctaaat ttgaaacctt ccctgtttgg aatcttcccc ttcatcatcc
     2881 agttaacttg gcttatgaag ctgccacagc tgaccttgat gatgtcaaca tgattgaccc
     2941 cttccatctt caaacctatg gagaaaccac tgtcaactac aaccgtgata tcgaaatctt
     3001 cccagtgctc aagcgcatgt tggaacgtat tctcggaaaa tcaccatacg cttcaccgac
     3061 agatatgggt gtcaacatgg ttggtttcgc tattacagat gacgaggctg ctgtcgaagc
     3121 ttctaaacaa gaaatcatcc gccgttacta tcaaacagtt cttgacttca aagctgaaaa
     3181 agttggcgaa gctgccgtca agaaaattga gttgctcatg aacgacctcg gtatctcacc
     3241 tgcagaccgt aaggttgctg tcgttgcgcg ccaaaaagca gaagaaactg gcggaccagc
     3301 cctagctctt gaattaccaa atggtgacat tgtcactggt aaaaactcag aactctttgg
     3361 ccctacagcc gctgccttga tcaacgccat caaaaaatca gctgacatcg ctaaagaagt
     3421 aaaactaatc gagcctgaag ttgttaagcc aatccaagga cttaaaatcg atcatctcgg
     3481 tagccgcaat ccacgccttc attcaaatga aatcctgatt gcacttgcta tcacagctac
     3541 agaaaatcct gatgctgccc gcgctatgga agaactcggc aacctcaaag gaagcgaagc
     3601 ccactcaacc atcatcttga ctgatgaaga caagaatgtc cttcgtaaac tgggtatcaa
     3661 cgtaaccttt gacccatact accaatacga ccgcttatat cgtaagtaaa gatttcaacc
     3721 tctccagcct cggagaggtt ttctctctgt ctcaggagca agctttatga tataatgaga
     3781 gaagaaaact gaaaggattt accatgtcaa aagaagttat tgtcgaaagt tttgaacttg
     3841 accacaccat tgtcaaagca ccctatgttc gcttgattgg ggaagaaaca ggaccaaaag
     3901 gagacatcat ctccaattat gatattcgct tggtgcaacc aaacgaagac tctatcccta
     3961 ctgccggcct tcacactatc gagcacctct tagccaaact catccgtacc cgcattgacg
     4021 gcatgattga ctgttcacca tttggttgcc gcacaggctt ccacatgatt atgtggggac
     4081 gtcacaccag tgctaaaatc gcagctgtta tcaaggattc gctcaaggaa atcgctgaaa
     4141 ctactacttg ggaagatgtc ccagggacaa ccatcgaatc ttgcggaaac tacaaggacc
     4201 acagcctctt ctctgctaaa gaatgggcaa aactcatctt ggaacaaggg atttcagatg
     4261 atgcctttga acgtcatgtg atttaaaatc gagtag
//
LOCUS       DAVBCB010000091         4116 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVBCB010000091 DAVBCB010000000
VERSION     DAVBCB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.091"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..776
                     /locus_tag="VQU28_002033"
     CDS             <1..776
                     /locus_tag="VQU28_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477321.1"
                     /translation="DYSIVEEKLDWQSKLSKIYPNVEFIKIEKLKLILLCKYRNQIID
                     LEDDKKRLLLLKLLYLTSKQFCYDEFKGAKFGCMSFFSHYQGYMSRLDSLDSDKFVSV
                     KQRIVSSISKLVEQYSDHIINLDRTLVDSLSDTLENQLFDDWDYILSEITTYSELLAK
                     SGLLYNSLDYKIDNVKKEVAKLSDFHQSLFKIEGFSDFFDSWQFQRYRSLVNQVYEIF
                     SMLQVSSVERTVLCGALSTIVFSKSNFNYQAFFEYLKDT"
     gene            805..1713
                     /locus_tag="VQU28_002034"
     CDS             805..1713
                     /locus_tag="VQU28_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477322.1"
                     /translation="MDYVDVILDEYGSVFLIKDHIKLKINNLELYRYFSSFGMSHLQF
                     RKEELPGKLKQFDFEKNKSDSYFSKKSSLIDFIKRNYFYDIKLYSDNPKNPIESWKNK
                     TEKIKIAKSTEILWSEVANFSSISKSPYYCLLYNLKNTENYILIISKTYFSAIQKMVS
                     YNLNNYFELEDVCCKSIVSKRELIENSININKSKMKLLNVCEGSKGYTRRVFKYNNIF
                     VGQLEFHSNIIFSEIALNELSIVKLLDSVESVLSEQLFGKLNWDNLFVVLSDDFNDYL
                     DINESQKYYSNGSLRGLELGIFMGGV"
     gene            1715..3613
                     /locus_tag="VQU28_002035"
     CDS             1715..3613
                     /locus_tag="VQU28_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HET2477323.1"
                     /translation="MRIIEIVDVEDQLLQLLSAHTNIDIRAQELSTLDPDSKVDLIIY
                     LKSRRDIENINNIIIKSPNRNIIVIISAFDIIYALYFPKSTFRPCLNCLLLKLKDNNP
                     EHNLLLFLKYGTLPSKHFNLDNRLKSNLILFNEKLDPKVEEYSIEGVNTTNFTFHFSC
                     SCENSGYDMSERMADILLGEGKKLSPKNYRLKKSEHLFDFTRYLDRNFGFFHHSYDEK
                     ISEYAPLHAVEINVDAGYESGWGRTFMTEAATKSAFLESLERYCNSRNRRYKKLIRGC
                     FSDLSKKYNTLNPVDFCLSNKEYRDSSYKFHDFDSEKNYDWIFGKNITTGKICLLPSQ
                     LVFYKDFSKSEENERFVYETSNGCALGSSIEEAIFYGILEIIERDNFLLTWYLKEEVK
                     QLSWESLPSRLRIINERLKEDNCELKLFNITKEVGIPVIWALYINHNENASIRSFSAA
                     GCHINPLSAIESAIFEVITSVPTFERLIEVRNKESFIEEIQNNFSLIRDIDDHILYHA
                     HADYKNKMPFLFKNNYSEYQNEFDENRIFNTYSHATLTSDLQALIKIIEKKFGDIYVV
                     NISDKVSEKFGLYCVKVLIPGLLNMSFGHSNRRVNIERIKNYVDYRGGNVNSITLNSI
                     NHLPHPFP"
     gene            3637..>4116
                     /locus_tag="VQU28_002036"
     CDS             3637..>4116
                     /locus_tag="VQU28_002036"
                     /inference="COORDINATES: protein motif:HMM:NF025394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HET2477324.1"
                     /translation="MKRDWKSKHLFYHDVQQFDKLVIYLSKFMDKLVNQNQIEKYFYI
                     RYWEGGPHIRLRYLVRDTQNFETIDSMINKYAYKFWENNPPMKLLNKTDYKKIYNESI
                     DNNGKMDRKLRKNATIENIAYIPEIERYGGEEALIVSEDLFCICSQVSTALLNVVDNY
                     "
BASE COUNT         1539 a          443 c          712 g         1422 t
ORIGIN      
        1 cagattactc aatagtggaa gaaaaattag attggcaaag taaattatct aaaatttatc
       61 cgaatgttga atttataaaa attgaaaaat taaaattgat tttgttgtgt aaatatagaa
      121 atcaaattat tgacttagag gatgataaaa aaagattgtt actattgaaa ttattatatt
      181 tgacttcaaa gcaattttgc tatgatgaat ttaaaggagc gaaatttgga tgtatgtcat
      241 ttttttcaca ttatcaaggt tatatgagca gattagattc actcgactca gataaatttg
      301 tttcggttaa acagaggatt gtttcctcta tttctaaatt agttgagcaa tattcagatc
      361 atattataaa tctggataga actttagttg attctctaag tgataccctt gagaatcagc
      421 tatttgatga ttgggattat atattaagcg aaataactac atatagcgaa ttattagcaa
      481 aatcaggatt gttatataat tcgctagact ataagattga taatgtaaaa aaagaagtgg
      541 ccaagctaag tgactttcac caatctttat ttaaaattga aggattttca gatttttttg
      601 atagttggca atttcaaaga tataggtctc tcgtgaacca agtttatgaa attttttcaa
      661 tgttacaagt aagtagtgta gaaagaactg tgttgtgcgg agctctaagt actattgtct
      721 ttagtaagtc aaattttaac tatcaagctt tttttgagta tctaaaagat acgtagaatt
      781 cgattttaaa ggaggctgaa atatatggat tatgtagatg taatattgga tgagtatggg
      841 agtgtttttc tgataaagga tcacataaaa ctaaaaatta ataatctaga actatatcgg
      901 tattttagta gctttggaat gagtcatctt caatttagaa aagaagagct tccaggaaaa
      961 ttaaagcagt ttgattttga aaaaaataaa agtgacagtt atttttctaa gaaaagtagt
     1021 ctgattgatt ttattaagag aaattatttt tatgatatta agttatattc tgataatccg
     1081 aaaaatccga tagagtcttg gaaaaataag acagaaaaaa ttaagattgc taaaagtaca
     1141 gaaattcttt ggagtgaagt agcaaatttt tcgtcaattt ctaaatctcc ttactattgc
     1201 ttgttatata atttaaagaa tactgaaaat tacatattga taatcagtaa gacatatttt
     1261 tcagccatcc aaaaaatggt ttcgtataat ctaaataatt attttgaatt agaagatgtt
     1321 tgttgtaaaa gcatagtgag caagcgagaa ttgatagaaa attcaattaa tattaacaaa
     1381 agcaaaatga aactgctaaa tgtttgcgaa ggtagtaaag gttacacaag aagagtattt
     1441 aagtacaata atatttttgt gggacaattg gaatttcact ctaacataat cttttcagaa
     1501 attgctttaa atgaattaag tattgttaag cttttagatt ctgttgaaag tgttttatct
     1561 gagcaactat ttggaaaatt aaattgggat aatttatttg ttgttttatc agatgatttt
     1621 aatgattatc tggatataaa tgaatctcaa aaatactact caaatggttc tttgagagga
     1681 ctggagcttg gaatctttat gggaggggtt taaaatgaga ataattgaaa ttgttgatgt
     1741 tgaggaccaa cttttacaac ttttaagtgc acataccaat attgatatta gagcacaaga
     1801 gttatcaaca cttgacccag attcaaaagt tgatttgatc atatacttga aatctcgacg
     1861 tgatattgaa aacataaata atattattat taaatcacct aacaggaata tcattgttat
     1921 aataagtgct tttgatatta tatatgcatt gtattttcca aaatcaacat ttagaccttg
     1981 tttgaactgt ctattattaa aacttaaaga taacaaccct gagcataatc tattattatt
     2041 cctgaaatac ggaacattac ctagtaaaca ttttaatctt gataataggt taaaatcaaa
     2101 tttaatacta tttaatgaga agttagaccc taaggtagag gagtatagta ttgagggggt
     2161 aaatactaca aattttactt tccattttag ttgtagctgt gaaaatagtg gttacgatat
     2221 gtcggaaaga atggcagata ttctacttgg tgaaggaaaa aaattatctc ctaaaaatta
     2281 tcgtttaaaa aagagtgagc atctattcga ttttactaga tatctagata gaaattttgg
     2341 attttttcat cattcatatg atgaaaaaat ctccgaatat gcgcctctcc atgcagtaga
     2401 gattaatgtt gatgctggtt acgagtcagg ctggggaagg acttttatga cagaggctgc
     2461 aacaaaatct gcttttttag agtcattaga gagatactgc aactctagaa atagacgcta
     2521 taaaaaatta attagagggt gtttttctga tttatctaaa aagtacaata cattaaatcc
     2581 tgttgatttt tgtctttcta ataaagaata tagagatagt tcctataaat ttcatgattt
     2641 tgattctgag aaaaattatg attggatatt tggtaaaaat ataactaccg gaaaaatttg
     2701 cttattgccc tcgcaactag tattttataa agactttagt aaaagcgaag aaaatgaaag
     2761 gtttgtttat gaaacatcaa atggatgtgc tttggggagt agtattgagg aagctatttt
     2821 ttatgggatt ttagagatta ttgaaagaga taatttttta cttacatggt atttaaaaga
     2881 agaagtaaaa caattatcat gggaatcttt accatctcgc ctacgtatta ttaatgaaag
     2941 actaaaagaa gataactgtg agctcaaact ttttaatata actaaagaag tgggcatacc
     3001 agtaatttgg gcattgtata taaatcataa tgaaaatgct agtattcgaa gtttttcagc
     3061 tgcaggctgt catattaatc ctttaagtgc aattgaatca gcaatttttg aagtaataac
     3121 ttctgtgcca acttttgaaa gattaattga agttagaaat aaagagtcat ttattgagga
     3181 aattcagaat aatttttctt taatacgaga tattgacgat catatattgt atcatgctca
     3241 tgcggattat aaaaataaaa tgcccttttt atttaaaaat aattatagtg aatatcaaaa
     3301 tgaatttgat gagaatagaa tttttaatac ttattcacat gcaacgctta catcagattt
     3361 acaggcacta ataaaaatta ttgaaaaaaa atttggagat atatatgtag tgaatataag
     3421 tgataaagtt tccgaaaaat ttggacttta ttgtgttaaa gtgttgattc ctggacttct
     3481 taatatgtca tttggacatt caaatcggcg tgtaaatata gagaggataa aaaattatgt
     3541 agattataga gggggaaatg taaatagtat tacattgaat tccataaacc atcttcccca
     3601 tccatttcca taagattata tataaaggaa taataaatga aaagagattg gaaatcaaag
     3661 catttatttt atcatgatgt tcaacaattt gataaattgg taatctattt atcaaaattt
     3721 atggataaat tagttaatca gaatcagatt gaaaaatatt tctatattcg ttattgggaa
     3781 ggggggcctc atattcgatt aagatatttg gtgagggata cacaaaattt tgaaacaatt
     3841 gatagtatga taaataagta tgcttataaa ttttgggaaa ataatcctcc aatgaagtta
     3901 ttaaataaga ccgattataa gaaaatttat aatgagagta ttgataacaa cggaaagatg
     3961 gatagaaaat tgaggaaaaa tgcgactatt gaaaacattg cctatatacc ggaaatagag
     4021 agatatggcg gggaagaagc tctgatagta agcgaagatc tattttgtat ctgtagtcag
     4081 gtttcaactg cgttacttaa tgtggtagat aactat
//
LOCUS       DAVBCB010000092         4026 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVBCB010000092 DAVBCB010000000
VERSION     DAVBCB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.092"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..370)
                     /locus_tag="VQU28_002037"
     CDS             complement(<1..370)
                     /locus_tag="VQU28_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2477325.1"
                     /translation="MLLVLVSLVHSFGSVSIIAHTIHQKFNLKVPNYRQEEDWARMGL
                     PITRKEISNWHIKASQYYLESLYKLLREKLLEQPLLHADETSYRVLESDSHLTYYWTF
                     LSGKAENQAITLYHHDQRRSG"
     gene            complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQU28_002038"
                     /pseudo
     CDS             complement(446..1297)
                     /gene="rfbD"
                     /locus_tag="VQU28_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
     gene            complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQU28_002039"
     CDS             complement(1363..2412)
                     /gene="rfbB"
                     /locus_tag="VQU28_002039"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HET2477326.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLVAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGIYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            complement(2422..3018)
                     /locus_tag="VQU28_002040"
     CDS             complement(2422..3018)
                     /locus_tag="VQU28_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HET2477327.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     VLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENLEEADVSEADKHHPLLKDVKPLKKEDLE"
     gene            complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQU28_002041"
     CDS             complement(3019..3876)
                     /gene="rfbA"
                     /locus_tag="VQU28_002041"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HET2477328.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGIKLSYAEQPSTDGLAIGEEFISDDSVALIL
                     GDNIYHGPGLSTMLQKAAKKEKGATVFGYQVKDPERFGVVEFDTDMNAISIEEKPEYP
                     RSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRGFAWL
                     NTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKNEYGQ
                     YLLRLIGEA"
BASE COUNT         1124 a          924 c          731 g         1247 t
ORIGIN      
        1 aaccactccg acgctgatca tggtggtaca gcgtgattgc ttgattctca gctttcccag
       61 acaaaaaagt ccaatagtag gtcagatggc tatcactctc tagaacccga taagaggttt
      121 catccgcatg aagaagaggt tgttctaaca acttttctcg taaaagctta taaagggact
      181 ctaaatagta ttgacttgcc ttgatatgcc aattagagat ttccttacgt gtgattggta
      241 aacccatcct agcccaatct tcttcttggc gataattggg taccttcaga ttaaacttct
      301 gatggatggt gtgagcgata atagaaactg agccaaagct atgcaccaaa gagaccagca
      361 ccaacaacaa gataattgta catcttaagc tcctttggag ttcattatct gcctctttta
      421 ttagactaga aaatcattct actacttatc tcacttcttg tttgtaaaat tcttgcaatg
      481 catcttgcca agttggaata acaaatccag tagctttggc tttggccagg ctcatcgttg
      541 agtttagcgg acgttgagct ttggctggaa attgactgga atctactggc ttgacttcga
      601 catctgtatc tttcaaaatt tcaactgcaa aatcatacca tgttgtgtct tctgtcgcat
      661 catttgacaa atgataataa ccaaattcct tacgattttc agctaggtag atcatgaatt
      721 cagccaaggt acgagtccaa gtcggacgac cgtactggtc atttacaact gttaaagtct
      781 tatgagtttt cgcaagattt tgcatggtaa aaacgaagtt tttgccataa tttccaaata
      841 cccaggcagt acggataata tagaaattag acacatgctt ctcaactaac tcttccccca
      901 tacgcttagt gcgtccatat tctgtctgtg gatctggtcg gtcatcaact tcccactctt
      961 gtccaactgg tttcttaccg tcaaagacct agtccgtaga aatataaact agagttgcac
     1021 catgcttttc agatgctttt gcgacatttt ttgtccccat cacattgatg gcgaagtcca
     1081 actcttttcc ttcatcctct gctgcatcaa cagcggtgta ggctgcacaa tggtagacta
     1141 aagtcggttt cacctcttca aaaactttct caaccatttc ttcattggta atgtccatct
     1201 cagccacatc tactgccacg tattcttcat tacgttcatc caataaatag cgaagttccg
     1261 ttcctaattg gccatttgcc cctgtaatta aaatcatctc tagacctttc taatatgtaa
     1321 ctcttcccaa tatagcaata aaaagcagcg atttcctgct ttttatactg taataatctc
     1381 ctgagtctta gcataattgg cttcaacagc ttctttttct gctttccacc attcttggtt
     1441 atctgtatac cacttgattg ttgccttgag cccagcttca aagttggtaa attcaggttt
     1501 ccaccccaac tcatcacgga gcttgctggc atcaatcgca tagcgaaggt catgtcctgc
     1561 acggtcagtc acatgatcat aggcatccgt agcttgtccc atttccttaa ggataagttc
     1621 caaaacttcc ttattgttct tctcaccatc agccccaatc aagtaggttt caccgatttg
     1681 cccttttgtc aagattgtcc aaactcctga agaatggtca ttggtatgaa tccagtcacg
     1741 aacgttctta ccttcaccgt aaagttttgg cttaattcca cttaggatgt tagtaatctg
     1801 acgtgggatg aatttttcga tatgttgata aggaccgtag ttatttgaac agttggaaat
     1861 cgttgccttg actccaaaag aacgcaccca ggctttgaca atcaagtctg atgcggcctt
     1921 ggttgatgag tacggagagc ttgggttgta ttttgtttca gcagtaaatt tctcacctgg
     1981 tccttcacca tgacctggca aatcttctcg taaagggaga tccccataaa cttcatctgt
     2041 cgatacatgg tggaagcgaa tatcatactt acgagcagct tctaaaagag tatagattcc
     2101 aatgaagtta gtatgaataa atggcgatgg atcattgagc gaattatcat tgtggctttc
     2161 cgctgcataa tgaacgatag catctgcttg agcaaccaac ttgtctacca actccgcatc
     2221 agcaatgtca ccaacaacta actcaacacg attgcctaaa atttcctcaa tattcgcgcg
     2281 gtttccagca taagtcaact tatctaggac tgtcacgtga acatctggaa agttctcgta
     2341 aacataatgg acaaagttag aaccgataaa gccagctcca cctgtcacga taatattttt
     2401 gtattcagtc atattctttc cttattccaa atcttctttt ttcaaaggct tcacgtcctt
     2461 aagtagggga tgatgtttgt ctgcttcaga tacgtctgct tcttccagat tttcccattc
     2521 aataccaagg cttggatcag cgtagttcac aaaggcatac ttgggtttga gttcaagagc
     2581 ccagtaatca ttgaccagat agctatatga cactgtatct gataaaactt ggaagccatt
     2641 agctacgcct cgaggaacaa agattccctt gcttgcatca attactgtct gataggtatt
     2701 cccaaaggtt tcaccctcgc gtagatcaac ccaagaaccc agaactttcc ctccatctgc
     2761 tacagagatg tacttatccc aaggctctgc gtggaggcct cgaaggacat ttttacgtga
     2821 gaaggataca ttgttttgca attttccttc tgcaaagaaa gactctggaa atccaagtgg
     2881 aagcactttt tccttttgga aattttcttt aaaccagcca cgattatctc catgaacggg
     2941 gatatcaaac tccaacatgc ctggaatagc ttcaaccttg cgtgccgcaa gcgtcttacc
     3001 gaaaaaatta tctgtcatct atgcttctcc aatcaaacgg agcagatact gtccgtattc
     3061 atttttctta agtggttggg ctaaggccaa tacatcttct cgactgatat agcccatacg
     3121 gtaagcaatt tcttctaagt ttgctacctg aacattttgc atccgttgca ctgtttcgat
     3181 gtactgtgaa gcctctagta aactttcatg agttccagta ttcagccaag caaagccacg
     3241 tcccataagc tcaacagaca aatcaccacg atctaggtaa gccttgttta catctgtaat
     3301 ttccagttca ccacgagggc ttggtttaat actcttggca atttcgacaa catcgttatc
     3361 atagaaatac agtcctgtca ctgcatagtt ggagcgagga tactccggtt tttcttctat
     3421 ggaaatagca ttcatgtctg tatcaaactc aaccacacca aaacgctctg gatccttcac
     3481 ttggtagcca aaaacagtcg cacctttctc tttcttggct gctttttgaa gcattgtgct
     3541 cagaccagga ccatggtaga tattgtcgcc taaaatcaag gcaacgctat cgtcagaaat
     3601 aaactcttcc ccaattgcca aaccatctgt acttggttgc tctgcataag aaagtttgat
     3661 cccaaactca gatccgtctt gaagaagctc ttgaaatcga tgtaaatcct gtggagtgga
     3721 aataatcaaa atatccctaa tcccagccaa catcaatgtt gaaagtgggt agtaaatcat
     3781 cggtttatca taaaccggca taagttgttt tgacgcagcg cgagtcaaag gatataaacg
     3841 tgtccccgaa ccacctgcta gaataatacc tttcatagta gggtaccttt ctttgttatt
     3901 ttagagtcaa ttactattaa ttgtgtactg ctctaaaaag aaaacaatca tcccatgttt
     3961 tatatggctc tataatattt gtagtgggta aatccactat agatattatg gagcctattt
     4021 tattgt
//
LOCUS       DAVBCB010000093         4016 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVBCB010000093 DAVBCB010000000
VERSION     DAVBCB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.093"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            98..1357
                     /locus_tag="VQU28_002042"
     CDS             98..1357
                     /locus_tag="VQU28_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HET2477329.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQIQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    1433..1568
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            1607..2107
                     /locus_tag="VQU28_002043"
     CDS             1607..2107
                     /locus_tag="VQU28_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HET2477330.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            2183..2551
                     /gene="rplL"
                     /locus_tag="VQU28_002044"
     CDS             2183..2551
                     /gene="rplL"
                     /locus_tag="VQU28_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HET2477331.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKTKLEEAGASVTLK"
     gene            2738..2908
                     /locus_tag="VQU28_002045"
     CDS             2738..2908
                     /locus_tag="VQU28_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477332.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            2908..3156
                     /locus_tag="VQU28_002046"
                     /pseudo
     CDS             2908..3156
                     /locus_tag="VQU28_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            3170..3510
                     /locus_tag="VQU28_002047"
                     /pseudo
     CDS             3170..3510
                     /locus_tag="VQU28_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3503..3883
                     /locus_tag="VQU28_002048"
     CDS             3503..3883
                     /locus_tag="VQU28_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HET2477333.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            3896..>4016
                     /locus_tag="VQU28_002049"
     CDS             3896..>4016
                     /locus_tag="VQU28_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2477334.1"
                     /translation="MAERFWENLSIILAERNISWIELTRKMFAGEFHYPSELNR"
BASE COUNT         1287 a          710 c          860 g         1159 t
ORIGIN      
        1 agatagaact gacgaagtca gtaaccacac ctacggcaaa gtgaatctga agtggtttga
       61 agagagtaca acttgtcttt tagaaaagga gcctataatg aaagtctttc agcatgtaaa
      121 tatcgtgact tgtgatcaag atttccatgt ttatcttgat ggaatcttag cagtcaagga
      181 ttctcaaatc gtctatgtcg gtcaagataa gccagcgttt ttagagcaag ctgagcagat
      241 tatagactat cagggagctt ggattatgcc tggtttggtc aattgtcaca cccattctgc
      301 aatgacaggt ctgagaggga tccgagatga cagcaatctc catgaatggc tcaatgacta
      361 tatctggcca gcagaatctg agtttactcc cgacatgact accaatgcgg tcaaagaagc
      421 cctgacagag atgctccagt caggaacaac aacctttaac gatatgtata atcccaatgg
      481 tgtggatatc cagcaaattt atcaggtggt gaaaacttcc aagatgcgtt gttatttttc
      541 tccgactctc ttttcttcag agacagaaac aactgctgag actataagca gaactcgatc
      601 catcatagac gaaatcttaa aatataaaaa tccaaatttc aaggttatgg tagcacctca
      661 ttctccgtat agctgcagta gagacttgct ggaagcgagt ttggaaatgg caaaagagct
      721 aaatattccg ctccatgtcc atgtggcgga gaccaaggag gagtcaggaa ttatcctcaa
      781 acgatacggc aaacgccccc ttgcttttct tgaagaactg ggttatttag atcatccgtc
      841 cgtatttgct cacggggtcg aattaaacga gagagaaatt gaacgcttgg catcttctca
      901 agtggctatc gcccacaatc ctatcagtaa cctcaaactg gcatcaggaa ttgctccaat
      961 tatccagatc caaaaagcgg gagtagcagt cggaattgcg actgactcgg ttgcttccaa
     1021 taacaatcta gatatgtttg aggaaggaag gactgcagct cttcttcaga agatgaaaag
     1081 tggggatgcc agccagtttc caatcgaaac agctctcaag gtactgacaa tcgaaggggc
     1141 taaggtcctt ggaatggaaa atcagatagg aagtctggaa gtcggcaagc aagcagattt
     1201 tctggtcatt caaccacaag ggaaaattca tctccaacct caggaaaata tgctgtctca
     1261 cctggtttat gcagttaaat ctagtgatgt agatgatgtt tatatcgccg gagaacaggt
     1321 tgttaagcaa ggtcaagtcc tgacagtaga actttaaaag aaaaatcacg aaaaatttta
     1381 aaaaaagttt gcaaaaatct tgcattcttt ttttgactat gctatactta tatacggttt
     1441 aaaaaaactg cctaagacag taggggagct cgactcataa atatcctacc gaggacaaga
     1501 cgtatcatgt aaaaagaagc gtattgtact ttcgtgtcta ggtttgggcg cgtttttctt
     1561 tttgaaaaat tccccaagca aaataattac ggaggtgaac acactaatga gtgaagcaat
     1621 tattgctaaa aaagcggaac tagttgacgt agtagctgaa aaaatgaaag ctgctgcatc
     1681 tatcgtcgtt gtagacgctc gtggtttgac agttgagcaa gatacagttc ttcgtcgcga
     1741 gcttcgtgga agcgaagttg agtataaagt tattaaaaac tcaatcttgc gtcgtgcagc
     1801 tgaaaaagct ggtcttgaag atcttgcatc tgtatttgtt ggaccatctg cagtagcatt
     1861 ttctaatgaa gatgttatcg caccagcgaa aatcttgaac gacttttcta aaaacgctga
     1921 agcacttgaa attaaaggtg gtgcaatcga aggcgctgtc gcatctaaag aagagattct
     1981 tgcacttgca actcttccaa accgcgaagg acttctttct atgctccttt ctgtacttca
     2041 agcgccagtg cgcaacgttg ctcttgcagt caaagcggtt gcagaaagca aagaagacgc
     2101 ggcttaatct taagctacac agcgtagcct agctacgaaa aaaactatta taaaatttaa
     2161 aacttatttg gaggaaataa caatggcatt gaacattgaa aacattattg ctgaaattaa
     2221 agaagcttca atccttgaat tgaacgacct tgtaaaagct atcgaagaag aatttggtgt
     2281 aactgcagct gctcctgtag ctgttgctgc agctgatgca gctgatgctg gtgctgctaa
     2341 agattcattc gacgttgaat tgacatctgc aggcgacaaa aaagttggcg ttatcaaagt
     2401 tgtacgtgaa atcactggtc ttggtcttaa agaagctaaa gaacttgttg acggtgcacc
     2461 agcacttgtt aaagaaggcg ttgcaactgc agaagctgaa gaaatcaaaa ctaaattgga
     2521 agaagctgga gcttcagtta ctcttaaata atagagtatc gcaatttgat tgcggaatcc
     2581 ctatttacca gtctttgaga gcgataagcg attgatagaa actgataaat tgatttggtt
     2641 tcctgccaaa aattctttgg caggattttt gttttaataa actttcgggg gtagacacta
     2701 gattgtctac cccttttttt gtgtttcgga ggtaattatg caaaaggaag gacagtcatc
     2761 acgtcaattc gtgaagagtc atctcatgac tatcatggga gttgatattg taaaagcaac
     2821 tcagttagtt gatgaaatgg aacaagtagg tttaatccga tttgatgaat ttggaaatgt
     2881 tggaatttta gtcttggagg gacaataatg aagtgtatta ccgctaatca atatcagaca
     2941 tcagagcgtt attataagct ccccaaactt ttatttgaga gtgaacggta taagaatatg
     3001 aaactggagg ttaaggtggt ttactcagtt ttaaaagatc ggttggagtt gtctttgagc
     3061 aaaggttgga ttgatgagga tggggctatt tatttgattt attccaattc aaatttgatg
     3121 gcacttttag gctgttcaaa gtcaaaatta ctctccatgt gagtttgaag tgacattttt
     3181 agatgattac cataaaaaac ataactaccc actattttac gaatcctatc ttcaaaacgt
     3241 tatggaattc cttgaaagtc aagacataaa gaatggggtt gatgcctttg tagatgatca
     3301 tcaaaatctc gtttttgttt tatatggaca aggctatcga gccgagggaa aagagggaat
     3361 acttacaacc caagtaactg taaaagctta tgatgaagac aagaaaccga ttaacttcgc
     3421 aaatttatta gattccttaa tttattagat tccttaatcg tgtcagaata tcaaatggaa
     3481 ccgaatcttt gggaggtctc ccatgattga tctctatcta agtaaaaata gacaaagaaa
     3541 tcaacttctt ttagatttct tccaaaacta tggcatagag gtatcttgtc attcagtttc
     3601 tgaaatgaca aaggacaaat taattgagat gatgagctat tcttcagatt gttttgagtt
     3661 tttatctccc aatttattac gatttaagaa ccgtgacaat ctaagattaa cggatttcgt
     3721 tgagataatt ttaaaaaatc ctgaactaac catcagactt cctcttgcgg tttcaaataa
     3781 gcgagtttat ccaagtctga atttggaaga ggctagagct ctattgccaa gagatacgaa
     3841 acaattgatt tacatggcgc aaacacatta tttatctaac taaaggagaa gcaatatggc
     3901 tgaacgattt tgggagaact tgtccattat tttggctgaa agaaatatca gctggattga
     3961 gttaaccaga aaaatgtttg cgggagagtt tcactatcca agtgaattga atcgtt
//
LOCUS       DAVBCB010000094         3262 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVBCB010000094 DAVBCB010000000
VERSION     DAVBCB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.094"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQU28_002050"
     CDS             complement(216..827)
                     /gene="rpsD"
                     /locus_tag="VQU28_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HET2477335.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(1022..2074)
                     /locus_tag="VQU28_002051"
     CDS             complement(1022..2074)
                     /locus_tag="VQU28_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HET2477336.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVTKLEKGQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2071..2769)
                     /locus_tag="VQU28_002052"
     CDS             complement(2071..2769)
                     /locus_tag="VQU28_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HET2477337.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2936..>3262)
                     /locus_tag="VQU28_002053"
     CDS             complement(2936..>3262)
                     /locus_tag="VQU28_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HET2477338.1"
                     /translation="VKETSDTANGSLSPSNSGSGVTPMNHNHATGTTDSMPADTMTSS
                     TNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGFLGLALAGLLGGLGLK
                     NKKEEN"
BASE COUNT          912 a          735 c          626 g          989 t
ORIGIN      
        1 ttttttacga aaatctattg aatatgccat aaaaggatta taccacattg tgtactattt
       61 ttggttcatt ttactatatc actttggact atatcggtct agttatgtct aaacgattat
      121 agctggatac taagagaaat caaaaaagca ctaagggaga acgtcccaaa gtgcttaata
      181 tcaaggcctc aaagcctgtc ttattcaata aaatattaca acatcttgtt gtagaattca
      241 acgacaagtg cttcgttgat ttctgggttg atttcgtcgc gttctggcaa gcgagtcaat
      301 gaaccttcca atttttcagc gtcgaatgat acgaatgctg gacgtccaag agtagcttct
      361 actgcttcaa ggattgctgg aactttcaat gatttttcac gaactgagat cacttgacct
      421 ggagttacgc ggtatgatgg gatatcaacg cgtttcccgt caacaaggat gtgaccgtgg
      481 tttacgaatt gacgagcttg acgacgagta gtcgcgagac caagacggta aacaacgtta
      541 tccaaacgac gttccaaaag aagcataaag ttgaaaccta ggattccgcc tttgattttt
      601 gtagcttgta cgaacaagtt acggaattgt ttttcaccta caccgtaagt gaaacgaagt
      661 ttttgttttt cagccaattg caaaccgtat tctgacaatt tagaacggtt gtttggtccg
      721 tgttgtcctg gtacgtagtt acgacgtgcc aattctttac ctgtacctgt aagtgaaagg
      781 ccaaggcgac gagcttgttt ccaagatggt cctgtataac gtgacatatg aatgtcctcc
      841 tgatataaat aatatttcgg tggaaatagt cacttagaaa gccctgattc gtgcagatgc
      901 ccttcgccta aacagccaag gttacttgtc ataagacacc tgttgacgag cttcatgctt
      961 tcctgctgat atttcacaca aaatccattt taccatgaat agctggattt gtaaaggggt
     1021 tttaggcttt attttcacta ccagagagat tgagaacgag ggtaaaggta cttcctagac
     1081 cgtactggct gctgactgtg atttccccac ccaattgatg ggccaattca cgcgcaatcg
     1141 caagtcctaa tccatgacca cctgtcttca tgttacgcga agtttcgaca cgataaaggc
     1201 gtttgaaaat attttccaaa tcctctgggg caatgccctg cccttcatcg gtcacactga
     1261 ttgaaagctg gcccttctcc agcttagtca ccacttccag cttggttcct ggagcagaat
     1321 atttaaaagc gttatcgacc agattcacca agatacgaga aagcttagca taatctccct
     1381 caatccgggc agactctggg attacctgca agtggacatc tcttctctcc tgctcaatca
     1441 aaaactgaaa ttcactcatg cactcaatta agagcttgtc cagaaaaata ctgtctttac
     1501 tggtagtttc cacctgattt ctagctgtgt ttagggtcaa aaaattcaac tcctcaacca
     1561 gtttattgag cctctccgtc tggcgtccaa tggttgctag ataatgagct tgctccgact
     1621 ccttgataat cccatccaaa atcccttcta ccgtcgcttg gatcgaagtg ataggggtct
     1681 taatatcatg cgacaactgg gcaatcatca agcccttttc tcgttcgctt tcttccaagg
     1741 aatcaaagct tacctgcaaa tcatgggaca tctcattaaa agtttgccct aattgctgaa
     1801 attctacagg accttgaacc tccaaatttg aaggaaaatc cttggccgct acccgcttgg
     1861 catgctcctt gagtttgccc aacgacgtaa agactggcaa taggagaaag agactaatcc
     1921 cagcaccgac aaggctggca acgatggtca tcccaagcaa aaagtaaatc tcgcctttcg
     1981 caatcagcat tttttgaaca gcccaaaaaa caaccaaaat ggttaagagg gttgaaataa
     2041 tatatccaac caaaatataa ctttttagtt tcatgtttgt cctctcggtt tctctatctt
     2101 atatcccaac ccccaaactg tcttaatagt gggcgtttgg tcactactat attttgccag
     2161 ctcctgtcga agagcatgga tatgcacatt caaggtattg gtgtcatcca cgtagtcttc
     2221 tttccagatc ttttcataga ggtctgtctt ggagaaaact cgctctggat tgctagctaa
     2281 aatccacagc aattcaaatg atttaacagt taaatccagc atttcttctc ctatttgaac
     2341 ttcatgacta ctatgattca tttttagatt gccaagggaa atcagctctg tttcgccccc
     2401 acgatgaagg cggcgcaaaa tattgtggac acgcaaaacc agctcacgtg ggctaaaagg
     2461 cttagcaata aaatcatctg ctcccaagct caggccgtaa atcttgtcct gttcactggt
     2521 cttagcagta ataaatagga aaggctgctc tggtgataag tattgaacct cactgattaa
     2581 atcataacca tccatccgag gcatcataac atctgtgata atcaaatcaa tcggtttttt
     2641 cttgaacagc tctagcgctt ccagtccatc atgggctacc aagacctgat aacctgcctg
     2701 aattaagtat ctctgatgaa tatctgtgat ttctacctcg tcgtcaacga gtaaaattgt
     2761 ctttcccatc tgtctctcct ttgataaaaa cagtgctata ctgctttaag tataacacta
     2821 tttttattgt tttacgatga ttttaatctg ctattgaaag aaagtcctga ttgctgattg
     2881 agttgttaaa ctaatcttag tcagcccact atccatcatt tatttcctta gctgattagt
     2941 tttcttcttt tttgtttttc aaacctagac caccgagtaa acctgcaagc gcaagcccaa
     3001 ggaaaccaat acttgccatt gatgtttgag tctcaccagt atttggtagc atagctttat
     3061 cctctgacat catcgtatca gacatcttgt tagcagaagc agccatgttt tcacctgcca
     3121 tcgtgttggt agaacttgtc atggtgtcag caggcatgct atctgtagta cctgtagcat
     3181 gattgtgatt catcggagtc acgccagaac cagagttaga aggtgataat gaaccatttg
     3241 ctgtgtctga tgtttcttta ac
//
LOCUS       DAVBCB010000095         2853 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVBCB010000095 DAVBCB010000000
VERSION     DAVBCB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.095"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(481..1206)
                     /locus_tag="VQU28_002054"
     CDS             complement(481..1206)
                     /locus_tag="VQU28_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477339.1"
                     /translation="MKLLKNELIKSKIFLFIIVDICIQILVILAIKTYILDISAPYSE
                     LDYNKYWYVLHALIYMPMIFPIQILYQNLREALIEDNNNGWNIMVINTNNLVKIIYIK
                     VTINIVRCFICYFVYTIFSLIQLGGMGTDMLLTNIIFPNIIFPNIMSFLLFLPIAIFM
                     QICCIRFDSILAKALPNILLILIVLITFQSDWNIFIPATYYYTEIQSTTNLGIKLLVC
                     IWIMGFEFSLLPKLIKLKEQNLV"
     gene            complement(1219..1956)
                     /locus_tag="VQU28_002055"
     CDS             complement(1219..1956)
                     /locus_tag="VQU28_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HET2477340.1"
                     /translation="MIKLEFLKQKKSILWFVLIFPIILNALLYIDLTFRYRGYLLVHQ
                     NELALSNWQLIFKEQTIFYFSELFYLVLSLIIYEVFAVEFKNDAWLTVISLPFRNKYT
                     INSKLLITVVYTFTFWLSDYISLYVIGKAIDNSLEIGLIFFLKTFTIQLISSLMIMLL
                     YFLTLVLIRKISGIIPIGIIMMILTISIYYNDYNFKIYLPFTYLSHAFRVTESQFYMI
                     LLSNIIIIVLFYILIRKLNERSFEMKL"
     gene            complement(1949..2677)
                     /locus_tag="VQU28_002056"
     CDS             complement(1949..2677)
                     /locus_tag="VQU28_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HET2477341.1"
                     /translation="MAIVEIINLTKSFKDIEVIHNTSFYLNKGKVYGFVGPNGAGKTT
                     IIKMILGILKPDSGKITIFNQTVEQNSKNILSRIGLVLGPSFYGHLDAYKNLKLIANM
                     KGLSLDTERLNEYLSMVGLKDVKKKKVKNFSMGMKQRLSIAASLLGSPEILIWDEPIN
                     GLDPQGVIEIRSLIRFLQEKKGITFLISSHILSELDKVISDIIIINYGKVEFFGSCHY
                     LLQKYNCRNLEEAYLACLAGGEYD"
     gene            complement(2711..2830)
                     /locus_tag="VQU28_002057"
     CDS             complement(2711..2830)
                     /locus_tag="VQU28_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001859549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HET2477342.1"
                     /translation="MIGIVFAIFLDFPVEYGISLGLLNGIVLGSLIVYKNNKN"
BASE COUNT         1084 a          435 c          343 g          991 t
ORIGIN      
        1 tggacatttt gaactaaaat taatttatta aacatatctc ataagcatat ctattatagg
       61 tgagacaata ttaagaaaga cggttaaaaa acaacctgac tccactggaa ttcaggaatc
      121 aggtcgctta agattttata ttattttact gtctacttga cagggagctg ttcaccaatg
      181 ggaatctata gctttttttg tgactgagaa ctattttatc tcagacttct tcaacacacc
      241 ttattattgg gcacttacgg aaggttaatg aattatttaa aataaaagta aagagaagca
      301 aacagacact ctatagctgt tagacgcttt ttagagtaat aacaatttga taaagtgaat
      361 acaacttgct taattaagcg taagaaacca gaatcataaa atcaattact ttgaagtcat
      421 gactcacgtt gtattgtttc attgttaatc gtattcaatt atttaatttt cgagcaacat
      481 ttaaactaga ttttgttctt tcaattttat tagttttgga agaagggaaa attcaaatcc
      541 cataatccag atacaaacta ataatttaat acctaagttt gtagttgact gaatttctgt
      601 ataataataa gttgcaggta taaatatatt ccaatcactc tgaaacgtga ttaaaacaat
      661 aagtatcagc aaaatattag gtaaggcctt tgctaaaatg gaatcaaatc ttatacagca
      721 aatttgcata aagatagcta taggtaaaaa taacagaaat gacattatat tgggaaatat
      781 tatattggga aatattatat tggttaataa catatcagtt cccataccac ctaattgaat
      841 taaagaaaaa attgtataaa caaaataaca aataaaacat ctcacgatat ttatcgttac
      901 ttttatatag atgattttca cgagattatt agtgttaatc accatgatat tccaaccatt
      961 attattatct tcaatcaatg cctctcttag attttgatac aaaatttgta taggaaaaat
     1021 cattggcata tatattaaag cgtgtaaaac ataccaatac ttattataat caagctctga
     1081 gtatggtgcc gaaatatcta aaatatatgt ttttatagct agaatgacta gaatctgaat
     1141 acagatgtca acaataataa ataaaaatat tttactctta atcaattcat ttttaagtaa
     1201 cttcattttt attacctatt aaagtttcat ctcaaaactt ctttcgttca atttacgaat
     1261 taatatataa aaaagtacga tgattattat attagatagt aaaatcatat aaaattgact
     1321 ttcagtcact ctaaaagcat gagaaagata agtaaatggt aagtaaatct taaaattata
     1381 atcgttgtag tatatgctaa ttgttaaaat catcattata ataccaattg gtattattcc
     1441 agaaattttc cttataagta ctaaagttag aaaatataat aacattatca tcaacgagga
     1501 aattaattga atggtaaatg tttttaaaaa gaaaatcaaa ccaatctcta aactgttatc
     1561 aatagccttc ccaataacgt ataagctaat ataatcagac aaccaaaaag taaatgtgta
     1621 aaccactgta atgagtaatt tagaatttat tgtgtacttg tttctaaatg gcaatgaaat
     1681 tacggttaac catgcatcat ttttaaattc tactgcaaac acttcgtaaa ttattaaaga
     1741 tagaactaaa taaaatagtt cagaaaaata aaatattgtt tgttctttaa aaattaactg
     1801 ccaattagat aaagcaagtt cattttgatg caccaataaa taacctcgat acctaaaagt
     1861 taagtctata tataacaaag cattcagaat tattggaaaa ataagaacaa accataagat
     1921 gctttttttc tgctttagaa actctaattt aatcatattc acctccagct aaacaagcta
     1981 agtaggcttc ttccagattt cgacaattat acttttgtaa aaggtagtgg caagaaccaa
     2041 aaaattcaac tttcccataa tttataatta taatatcaga tatcacttta tccagttcac
     2101 taagaatatg acttgatatt aaaaatgtaa tacctttttt ctcctgtaga aaacggatta
     2161 aacttctaat ttcaattaca ccctgtggat caagtccatt gattggttca tcccaaatta
     2221 atatttctgg actacctaac aatgatgcag ctatacttag acgttgtttc atgcccattg
     2281 aaaaattttt cacttttttc tttttaacat cttttaatcc taccattgat agatattcat
     2341 taagtctttc agtatctaaa ctaagccctt tcatatttgc tattaacttc aaatttttat
     2401 aagcatcaag atgaccataa aatgaaggtc ccaaaactaa tcctatccta gataatatat
     2461 ttttggaatt ctgttctacg gtttgattaa aaatagttat tttcccagaa tctggtttta
     2521 aaatccctag aatcattttg ataattgtag tttttccagc cccatttggt ccaacaaaac
     2581 catatacttt tcccttattt aaataaaatg aagtgttatg aataacttca atatctttaa
     2641 agctttttgt tagatttata atttcaacta tagccatctc tactccttag tttacagcaa
     2701 aaaattatgc ttaattctta ttgtttttgt aaacaatcag cgaacccaat actattccat
     2761 tcaacaaacc taaagaaata ccatattcaa ctggaaaatc taaaaaaatt gcaaatacta
     2821 ttccaatcat cacccctagt gaggctgctg ttg
//
LOCUS       DAVBCB010000096         2624 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVBCB010000096 DAVBCB010000000
VERSION     DAVBCB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.096"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            206..520
                     /locus_tag="VQU28_002058"
     CDS             206..520
                     /locus_tag="VQU28_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477343.1"
                     /translation="MWVLGFILFLIFFYSNDSKKIKKLEKKIKRLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRRVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            530..736
                     /locus_tag="VQU28_002059"
     CDS             530..736
                     /locus_tag="VQU28_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HET2477344.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            736..933
                     /locus_tag="VQU28_002060"
     CDS             736..933
                     /locus_tag="VQU28_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477345.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVIEWIEKKSKNLASEKGE"
     gene            989..1669
                     /locus_tag="VQU28_002061"
     CDS             989..1669
                     /locus_tag="VQU28_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HET2477346.1"
                     /translation="MGMFVGMFKARVESHEIILDVKALMPWISAICLLIGFISMFLTF
                     NFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAFNITGVVIPLAIFISLSEV
                     IILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLEFFATPKDVLNYINSYDEG
                     ERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQLLAFLLVGAIHVYINVMQL
                     PMVKRYFK"
     gene            1681..2322
                     /locus_tag="VQU28_002062"
     CDS             1681..2322
                     /locus_tag="VQU28_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2477347.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            2340..2570
                     /locus_tag="VQU28_002063"
     CDS             2340..2570
                     /locus_tag="VQU28_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477348.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
BASE COUNT          766 a          431 c          541 g          886 t
ORIGIN      
        1 atacctacgg caaggtgaag ctgacgtggt ttgaagagat tttagaagag tattaatgat
       61 agataaagtg gtcaggaacc tactcctgac ctttttcttt tgcaaaatga caaaaatcat
      121 aatttttttg acaactatac ttgtcaaaaa gaaaaagatg tgttacaatg taagcaagtt
      181 aagagaaaag aagaaaggag ctatgatgtg ggtactaggg tttatactat ttctgatttt
      241 cttttattct aatgattcta aaaaaatcaa gaaactagag aagaaaatca aaagacttga
      301 gcgaaaagag aaaggaaatg cagaaatgtc gagattatta caagaaatga ttggaaagga
      361 accaattata acgggagtgt atattgggcc agataactgg gaagttgtgg atgttgatga
      421 ggaatgggta aagctacgac gtgtagataa tacgggaaaa gaaaaattca agttgcaacg
      481 tattgaggat atccaaaccg ttgaatttga cggagagtag gtgtgtaaca tgcaactaaa
      541 aaatcgtcta aaagagcttc gagctcgcga tggtctcaat caaaccgacc tagccaaact
      601 ggcaggggtt tccagacaga ccattagcct actagaacgg gatgagtaca ccccatccat
      661 tatcattgcc ctgaaaatat ctcaaatttt caacgaaaca gtcgaatcgg tatttcgttt
      721 ggaggaggac gagtgatgaa caagtataaa gtgatttatt atgtagtggt catagcttta
      781 ttagtcagcg tatttctact gattgggatg gacctaagct ggtttaatcc ctatcaaagc
      841 gaccaatttg tttgggtcta ctttgctctc atcccagtaa ttgaatggat tgaaaagaaa
      901 tccaaaaatc tagcaagtga aaaaggagaa tgaatatgaa aaagaaacgt agattgttat
      961 ttttgatgtc tattgtcttg gggggttctt gggcatgttt gtagggatgt ttaaggcacg
     1021 tgtcgaatcc cacgaaatta ttttagatgt aaaagccttg atgccatgga tatcagctat
     1081 ttgtttactg ataggtttca ttagtatgtt tttgactttc aatttcttaa agaaaagcag
     1141 aaaatttcat tccttgtatc aagaggaaat ggatgacgat ctgaatgaaa cctattatgt
     1201 gcaaatgtat cggaatcttg agtttggaac cattgctttt aatattacag gtgtagtgat
     1261 tccattggct atttttattt cattaagtga ggtgattata ttgcatacaa accctcaaac
     1321 atttttcctt tctttcttac tctttgtggt attcttagtc gctcaaaaat ctctttttaa
     1381 aaccattgcg attgttcgtc agtttgattt ggaatttttc gctacaccaa aggatgtctt
     1441 gaactatata aattcttatg atgaagggga gcgtcaggct aatttggaac agagttttcg
     1501 aattttattc caattacacc aatatgtctt accagcctta tatatttttc ttattatcct
     1561 ttctttcttg acaggagaga ttcagttact agctttcttg cttgtaggag ccatccatgt
     1621 ttatatcaat gtgatgcagt tacctatggt aaaacgttat ttcaaataaa ggagttatct
     1681 atgaaattac ttaaaaacct tggctggttt cttctagccc ttctatcctt tttctttatc
     1741 tatggcttta ttcaggggct cgcgactgcg tcgcttgctt taggcgcttc accctatgct
     1801 gttactctac tctatgtggt cttggctgga gtatatgtgt acggcattta caaatggtat
     1861 cagaaagctc ctgtttatat tgagaagagc ggctttaacc gatttatttg gcttcctgcc
     1921 ttggtttggt tcttatctct ggtcgttcaa ttcttcttgc cagatgatcc ttcagtaaat
     1981 cagcaaatag cgacagactt gaccttgtct caaccacttt tctcattctt tgctgtggtt
     2041 atttttgctc ctttgacgga agagattgtt tttagaggga tgttagcacg ctatctcttt
     2101 cctaagcagg acaatagtaa acgaaccctg atttttcttc tggtatctag tcttctattt
     2161 gccttgattc attttccagg tgatgtgcaa caattttttg tctattttag ccttggtttt
     2221 agtttgggtt tggcttacat tagcagaaaa ggtctggtct acagtatttc tcttcacgct
     2281 ttgaataatt tagtcggctt tttgatgatt ctcatgctat aatagagtca ggaggtcaca
     2341 tgaaacgagt aattttatta gcagtgattc aggcagtcgt tctatttttc atcattggag
     2401 cgctagctta tgccttcaaa ggcgatttct tctataacta tctagcagtt gtctttgctc
     2461 ctattgcagg tgtactgcgt tttgggacgg cttacataac ggaaattgtc ttgcctcgaa
     2521 aggcagccga aatcgctgaa aagcgtaaag caggcaaaaa ttcaaaataa aagagtccgg
     2581 tgaagttcat cggatttttg tattatactc aatgaaaatc aaag
//
LOCUS       DAVBCB010000097         2607 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVBCB010000097 DAVBCB010000000
VERSION     DAVBCB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.097"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            114..1031
                     /locus_tag="VQU28_002064"
                     /pseudo
     CDS             114..1031
                     /locus_tag="VQU28_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(1071..2015)
                     /gene="manA"
                     /locus_tag="VQU28_002065"
     CDS             complement(1071..2015)
                     /gene="manA"
                     /locus_tag="VQU28_002065"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HET2477349.1"
                     /translation="MSEPLFLQSVMQEKIWGGTKLRDEFGYDIPSEKVGEYWAISAHL
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTILVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(2147..2428)
                     /locus_tag="VQU28_002066"
     CDS             complement(2147..2428)
                     /locus_tag="VQU28_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HET2477350.1"
                     /translation="MDTPDENGYVADDYRITYLEAHIKAMRDAIYKDGVDLLGYTTWG
                     CIDSVSAGTGEMNKRYGFIYVDRDNVGNGTLKRSKKKSFYWYMSFIAMV"
     gene            2493..>2607
                     /locus_tag="VQU28_002067"
     CDS             2493..>2607
                     /locus_tag="VQU28_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HET2477351.1"
                     /translation="MKSTKEEIQTIKTLFKDSRTAKYHKCLQIILFRLMGKS"
BASE COUNT          698 a          606 c          477 g          826 t
ORIGIN      
        1 aaaagtcgtg cgctgaacac caacaagacg cttaaatcgt gcatcagtta gttgtttact
       61 tgcttcatca ttcatagaac tactatgcca tattttgttt cgcaggaagt ctgatcattt
      121 caaatccagc cattgctcta ggagctcaag cgacctttat cctattgaat atcttcattg
      181 tatcacgtgg ggttcaaaaa gggattgaaa gagcttcgaa agtcatgatg cccctgctct
      241 ttatcatctt tgtcgtcatc atcgaacgct ctctcagttt gccaaatgcc atggaagggg
      301 ttctttactt cctcaaacca gatttttcaa aactgactag cgctggtctc ctctatgctc
      361 tgagacaatc tttctttgcc ctctcactag gggttacagc catgttgacc tatgcttctt
      421 acttggacaa gaaaaccaat ctggtccagt caagaatctc catcgtagcc atgaatatct
      481 cggtatccat catggcaggt ctagccattt tccaagctcg gtcccccttc agactgaata
      541 ttgaaggggg accgagcttg gtctttatcg tcttgcctca actctttgac aagatgcctt
      601 ttggaaccat tttctatgtc ctcttcctct ttgcgactgt cacttcttct gtcgttatgc
      661 tggaaatcaa tgtagacaat atcaccaatc aggacaacag caagcgtgct aaatggagtg
      721 ttattttagg aattttgacc tttgtctttg gcattccttc agccctatct tacggtgtca
      781 tggcggatgt tcacattttt ggtaaagcct tctttgacgc tatggacttc ttggtttcca
      841 atctcctcat gccatttgga gctctcttcc tttcactttt tacaggctat atctttaaaa
      901 aggctcttgc aatggaggaa ctccatctcg atgaaagagc atggaaacaa ggactgttcc
      961 aagtctggct cttccttctt cgtttcgtca ttccaatcat catcgtggtc ttcatcgccc
     1021 aatttatgta atcaaaagga cttgagtaat gaactcaggt cctttctttt ttatggatga
     1081 ctaacaatta attctaaatc ttgcccttct agagtccaag attcaacatc acttggtagg
     1141 ataaagtgtc tccctttttg gattggatag tttttcccgt caacagttag ttgaccttga
     1201 ccagccaaga cactcaacaa gctgtagtca gctgtctttt caaagttaac ttttccagta
     1261 atttcccatt tgtaaactgc gaagaaatca ttggatacaa ggatagtgga acgtagatca
     1321 tctgctttga ttgtcactgg acggctattt gcgggttcac caatgttcaa gacatcgata
     1381 gatttttcaa ggtgaagttc acgtaaattg cccttgtcat ccttacggtc aaagtcatag
     1441 acacggtaag tggtatcgct agactgctgg gtttcaagaa tcaagatacc cgccccgata
     1501 gcgtgcatgg taccgcttgg tacatagaag aaatctcctg ctttaactgg cacttttgtc
     1561 aacagcgcat cccagttctt atcctcgatt tgctggcgga gttcttcttt tgacttggca
     1621 ttgtgaccgt agataatctc tgaaccttca tctgctgcaa tgatgtacca acattctgtt
     1681 tttccaagtt cgccttcatg ctcgagtcca taagcatcgt ctgggtgaac ttggacactg
     1741 agccagtcgt tggcatcgag gatcttggtc aaaagtggaa atacaggttc tggacgattg
     1801 ccaaataatt cacggtgttc cgcatacaaa gtagcaagat ctgttccctc gtaacgacca
     1861 ttggcaactt tagaaacacc atttagatga gctgagatag cccagtactc tccgactttt
     1921 tcacttggga tgtcgtagcc aaactcatca cgtagcttgg taccacccca gattttttct
     1981 tgcataactg attgtaaaaa taatggttct gacatcttaa tctcctgtct gatttttctc
     2041 ccctcattat agcaaaaaaa gagttccatt tgaactcttt tttacatctt ataaagcagg
     2101 gagaagattt tataaaaata gtaaacaaat gtgctctact cgatgcttac accattgcta
     2161 taaatgacat gtaccaatag aaggacttct tcttgctacg tttgagagtc ccatttccta
     2221 cattatctcg atctacatag ataaagccat agcgcttatt catttcccct gtgccagctg
     2281 aaaccgaatc gatacagccc catgtcgtat aaccaagcaa gtcaaccccg tctttgtaaa
     2341 tggcatctcg catggccttg atgtgggcct ctaagtaagt aatccgatag tcatctgcta
     2401 cataaccatt ctcatccggt gtatccatag caccgagtcc attttctacg ataatactat
     2461 actaaaatca aaaagcatta tataatagtg atatgaaatc aactaaagaa gaaatccaaa
     2521 ccatcaaaac actttttaaa gactctcgta cagctaaata tcataaatgc cttcaaatca
     2581 ttctatttcg tctgatgggc aaatctt
//
LOCUS       DAVBCB010000098         2396 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVBCB010000098 DAVBCB010000000
VERSION     DAVBCB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.098"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(30..426)
                     /locus_tag="VQU28_002068"
                     /pseudo
     CDS             complement(30..426)
                     /locus_tag="VQU28_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(371..553)
                     /locus_tag="VQU28_002069"
     CDS             complement(371..553)
                     /locus_tag="VQU28_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HET2477352.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            910..>2396
                     /gene="pcpA"
                     /locus_tag="VQU28_002070"
     CDS             910..>2396
                     /gene="pcpA"
                     /locus_tag="VQU28_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HET2477353.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDGA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSKATGWVKDKGLWYYL"
BASE COUNT          837 a          359 c          396 g          804 t
ORIGIN      
        1 agccttcata gcgtggcatt tctttttact tccgtcacat caatcattac cgtgtcctca
       61 gaactgagag gagttcttga aatcgtaaca ccacctttga acaagagtta cttcaaccca
      121 ttagctccga cggattaagt tgctttcgtg aataccaaaa tcagccgcaa tttcttcata
      181 agtgcggtat tctcgcacat attgaagagt ggccataaga aggtcttcta ggcttaattt
      241 aggttttcgt ccaccttttg tgtgtttaag ttgataagct gtttttaaca cagctaacat
      301 ctcttcaaaa gtggtgcgct gaacaccaac aagacgctta aatcgtgcat cggttagttg
      361 tttacttgct tcataatttc gcagggagtc tattgactct ttggtaggtg tcaatgtttt
      421 tttcatctat cccgagaatt attttcccgc catttgtatt tgcaaatgct gagtaggttt
      481 cccagaaaga ctctggaaga ttgtttttag cttttttgta ttctaaatca accccttcaa
      541 attttaagtc catatttttc ctttacatct gttttttgtg gttctggtat ttgttcaagt
      601 tgagtgataa tatagcgaat tgaatttcga gagtttttac tcagttaatt tcttttttaa
      661 cccactttaa tttctttttt aacccacttt aatttctttt ttaacacggg ttaaaaaaga
      721 aattaaagtg ggttaatttt tcttgactta aaatttaaaa atgatatact gtgtttatgg
      781 agagatgaca atttataaaa tgtactactc tattctacta gataatgatt gaataaaact
      841 ttttataaca acggctagca aagttataat tttatatcta ttagaaaata aatatgtaag
      901 gaaatttaaa tgaaaaaaac tacaatatta tcattaacta cagctgcggt tattttagca
      961 gcatatgtcc ctaatgaacc aatcctagca gatactccta gttcggaagt aatcaaagag
     1021 actaaagttg gaagtattat tcaacaaaat aatatcaaat ataaggttct aactgtagaa
     1081 ggtaacatag gaactgttca agtgggtaat ggagttactc ctgtagagtt tgaagctggt
     1141 caagatggaa aaccattcac gattcctaca aaaatcacag taggtgataa agtatttacc
     1201 gttactgaag tagctagtca agcttttagt tattatccag atgaaacagg tagaattgtc
     1261 tactatccta gctctattac tatcccatca agcataaaaa aaatacaaaa aaaaggcttc
     1321 catggaagta aagctaaaac tattattttt gacaaaggca gtcagctgga gaaaattgaa
     1381 gatggagctt ttgatttttc tgaattagaa gagattgaat tgcctgcatc tctagaatat
     1441 attggaacaa gtgcattttc ttttagtcaa aaattgaaaa agctaacctt ttcctcaagt
     1501 tcaaaattag aattaatatc acatgaggct tttgctaatt tatcaaattt agagaaacta
     1561 acattaccaa aatcggttaa aacattagga agtaatctat ttagactcac tactagctta
     1621 aaacatgttg atgttgaaga aggaaatgaa tcgtttgcct cagttgatgg tgttttgttt
     1681 tcaaaagata aaacccaatt aatttattat ccaagtcaaa aaaatgacga aagttataaa
     1741 acgcctaagg agacaaaaga acttgcatca tattcgttta ataaaaattc ttacttgaaa
     1801 aaactcgaat tgaatgaagg tttagaaaaa atcggtactt ttgcatttgc agatgcgatt
     1861 aaacttgaag aaattagctt accaaatagt ttagaaacta ttgaacgttt agccttttac
     1921 ggtaatttag aattaaaaga acttatatta ccagataatg ttaaaaattt tggtaaacac
     1981 gttatgaacg gtttaccaaa attaaaaagt ttaacaattg gtaataatat caactcattg
     2041 ccgtccttct tcctaagtgg cgtcttagat tcattaaagg aaattcatat taagaataaa
     2101 agtacagagt tttctgtgaa aaaagataca tttgcaattc ctgaaactgt taagttctat
     2161 gtaacatcag aacatataaa agatgttctt aaatcaaatt tatctactag taatgatatc
     2221 attgttgaaa aagtagataa tataaaacaa gaaactgatg tagctaaacc taaaaagaat
     2281 tctaatcagg gagtagttgg ttgggttaaa gacaaaggtt tatggtatta cttaaatgaa
     2341 tcaggttcaa aggctactgg ttgggttaaa gacaaaggct tatggtatta cttaaa
//
LOCUS       DAVBCB010000099         2070 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVBCB010000099 DAVBCB010000000
VERSION     DAVBCB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.099"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(57..176)
                     /locus_tag="VQU28_002071"
     CDS             complement(57..176)
                     /locus_tag="VQU28_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HET2477354.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(280..429)
                     /gene="blpO"
                     /locus_tag="VQU28_002072"
     CDS             complement(280..429)
                     /gene="blpO"
                     /locus_tag="VQU28_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HET2477355.1"
                     /translation="MDTKMMSQFAVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(673..876)
                     /gene="blpN"
                     /locus_tag="VQU28_002073"
     CDS             complement(673..876)
                     /gene="blpN"
                     /locus_tag="VQU28_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HET2477356.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGRLLGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(892..1146)
                     /gene="blpM"
                     /locus_tag="VQU28_002074"
     CDS             complement(892..1146)
                     /gene="blpM"
                     /locus_tag="VQU28_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HET2477357.1"
                     /translation="MDTKIMEQFHEMDITMLSSIEGGKNNWQTNVLEGGGAAFGGWGL
                     GTAICAASGVGAPFMGACGYIGAKFGVDLWAGVTGATGGF"
     gene            complement(1296..2019)
                     /locus_tag="VQU28_002075"
                     /pseudo
     CDS             complement(1296..2019)
                     /locus_tag="VQU28_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          645 a          462 c          295 g          668 t
ORIGIN      
        1 attaaagaaa ctcccctgta aattaagcta gcaaatacag gggagaaatt tattttttag
       61 agagtactat ccgtatcctt tttggaagat tttgaaaata tttttctaat taagtcatcc
      121 atataaggac caaatatacc aactactaaa ccaataataa aacttttaaa atccataatt
      181 accaccaaca tattgctgca taggctacac ctccaagtat agctccacct gcagcaccag
      241 ttacacctat tcctatagca aatggtccca atagaaatgt caaaccgttg ttgcacaccc
      301 atcaattgcg ccatatgcaa cccctgctgc acaactaatt tttcttcccc aatcaatatc
      361 tccaccttca acgcaagcaa gcatttcatt atccataact gcaaattgtg acatcatttt
      421 tgtatccata tagtatatca cttttcagtt acggaacaag tttaatataa aaattatcaa
      481 aaaaacatag gcaataaaga gaaaaattaa tttatcatag attagaaata atatgacaaa
      541 acaattcaat gatgttaatt caatagtctt ttgtttttta tcggagatac ttatggatag
      601 ataaataaga taggtttgaa aagcgaagag aataataaag aatatagcct tcataaaatt
      661 tagctttcat ttttatgatg tagcggtata ggctaaatat ccacaaacca ctgctcctcc
      721 aattcctcct attgcagcgc cccatggtcc tagaagtctc ccatatttca ctccacccgc
      781 tgcacaacct aaagcagcaa ctacagctgc tcctccggaa ttacctccat aaacctcact
      841 cagcattgtt tcatttatat tacaataagt attcatacaa gtctcctttt attaaaatcc
      901 acccgttgcc cctgttactc ctgcccaaag atccacacca aatttagctc ctatgtatcc
      961 acatgctccc ataaatggtg ctccaacacc actcgcagca caaatagctg tccctagccc
     1021 ccagccacca aaagcagcac caccaccttc caagacatta gtttgccaat tattcttgcc
     1081 tccttcaata ctagataaca tagttatatc catttcatga aattgttcca taatttttgt
     1141 atccatgaca aatactcttt tttattttta atttttgtct tgttgtaact ttgacaagtt
     1201 tagtatatca tcgtttttta aaatttttca tccagatctt gaatagtcat cgaaacgtct
     1261 tgaattgcaa aaattacatt agacttcctg caaaactaga atcctagttc atgattgata
     1321 ataccagcac tcaaattcat tcgtaatccg aagcgtttac gatgatttcg ataggttgtt
     1381 gaaaacattt taaacgtttt tactttggca aagatgttct caaccttgct tctctcctta
     1441 gatagcgcat ggttacaggc tttatcttca actgttagcg gtttgagttt gctggattta
     1501 cgtgaagttt gtgcttgagg atatatcttc atgagccctt gataaccact gtcagccaag
     1561 attttaccag cttgtccgat atttctgcga ctcattttga acaacttcat atcatgacaa
     1621 tagttcacag tgatatccaa agaaacaatt ctcccttgac ttgtgacaat cgcttgagtc
     1681 ttcatagcgt ggcatttctt tttaccagaa tcattcgcta attctttttt tagggcgatt
     1741 gatttgtact tccgtcgcat caatcattac cgtgtcctca gagctgagag gagttcttga
     1801 aatcgtaaca ccactttgaa caagagttac ttcaacccat tggctccgac ggagtaagtt
     1861 gctttcgtga ataccaaaat cagccgcaat ttcttcataa gttcgatatt ctcgcacata
     1921 ttgaagagta gccataagaa ggtcttctag gcttaattta ggttttcgtc caccttttgc
     1981 gtgtttaagt tgataagctg tttttaatac agctaacatc tcttcaaaag tggtacgctg
     2041 aacaccaaca agacgcttaa atcgtgcatc
//
LOCUS       DAVBCB010000100         1671 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVBCB010000100 DAVBCB010000000
VERSION     DAVBCB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.100"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            100..1575
                     /locus_tag="VQU28_002076"
     CDS             100..1575
                     /locus_tag="VQU28_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HET2477358.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNIVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
BASE COUNT          474 a          335 c          334 g          528 t
ORIGIN      
        1 tccagtattt gacaatattg aagcagctat taaagaacac ggattaagct aattctaatt
       61 tgttaacatt catataacat aaaaaaggga ggaactgtta tggatgcaat ctttgaccta
      121 atcggaaagg ttttcaatcc cattttagaa atgggtggac ctgtcatcat gttaatcatt
      181 ttgacagtat tggctttact ttttggagtg aaattctcca aagcgcttga aggtggtatc
      241 aaacttgcca tcgctcttac aggtatcggt gctatcatcg gtatgctaaa tggtgctttc
      301 tcagcatcac ttgcaaaatt cgttgaaaac actggtatcc aattgagtat taccgacgtt
      361 ggttgggcac cactcgcaac aatcacttgg ggttctgctt ggacactata cttcttgctc
      421 atcatgttga ttgtcaacat agtgatgcta gctatgaaga aaacagatac acttgacgtt
      481 gatatctttg atatctggca cttgtctatc acaggtctct tgattaaatg gtatgctgat
      541 aacaatggtg tgagtcaagg ggtttcactc tttattgcta cagcagctat cgtccttgtc
      601 ggtgtgttga aaattatcaa ctctgacttg atgaaaccta catttgatga ccttcttaac
      661 gccccaagtt catcaccaat gacatcaact cacatgaact acatgatgaa cccagttatc
      721 atggttttgg ataagatttt tgaaaaattc ttcccaggcc ttgataaata tgactttgat
      781 gctgctaaat tgaacaagaa aatcggtttc tggggatcta aattcttcat cggtttcatc
      841 cttggtatcg ttatcggtat tatgggaact ccacatccaa ttgcaggtgt tgcagatgca
      901 gataaatggc gtcttgttat caaaggatgg ttgtctcttg gtttgactgc cggtgtatct
      961 ttggaactct tctcacttat cggttcatgg ttcatcgcag ccgtagaacc actatcacaa
     1021 ggtattacaa acgttgctac taaacgtctt caaggacgta aattcaatat cggtcttgac
     1081 tggccattca tcgctggtcg tgctgaaatc tgggcttgtg ccaacgtact tgcaccaatc
     1141 atgttgattg aagcagtgct tctttcaaaa gttggaaatg gtatcttgcc acttgcaggt
     1201 atcatcgcta tgggtgttac tccagctctc ttggttgtaa ctcgtggtaa attgctccgt
     1261 atgattatct tcggaacact cttgttgcca ctcttccttc tttcaggtac acttattgca
     1321 ccatttgcaa cagaacttgc taaaggtgta ggtgccttcc cagaaggtgt gagccaaact
     1381 caattgatta ctcactctac tcttgaagga ccaatcgaaa aacttcttgg ttggacaatt
     1441 ggtaacacta caactggtga tatcaaagca atccttggtg cagtagtctt ccttgtattc
     1501 tatatcggta tctttgcttg gtacagaaaa caaatgatca aacgtaacga agagtacgca
     1561 gcaaaagcaa aataatgcgc tcctataaaa tataaactca atgaaaatca aagagcaaac
     1621 taggaaacta gccacaggtt gctcaaaaca cagttttgag gttgtagata a
//
LOCUS       DAVBCB010000101         1651 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVBCB010000101 DAVBCB010000000
VERSION     DAVBCB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.101"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            108..407
                     /locus_tag="VQU28_002077"
     CDS             108..407
                     /locus_tag="VQU28_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic biosynthesis protein"
                     /protein_id="HET2477359.1"
                     /translation="MDSYAINFWESKGVGIDKKILEFIQKNITVIKNKNFLNKDFDIY
                     VKKIHNLFFVISTLVSKEQYMGIVSSQIHMFANRMGISPTIEVTMLDFFLREDSD"
     gene            409..1179
                     /locus_tag="VQU28_002078"
     CDS             409..1179
                     /locus_tag="VQU28_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HET2477360.1"
                     /translation="MEKYFFDISIFEKIKKVLSYQSKYWEISKPRDKYSFKSQNRISG
                     SNSIKFKDFELDYELESLGKILLKRRSFLLNEFDKNASFDKLIYLLKTVFSPTNCKFV
                     FGEEVPLFSYPTSGALNSITPFVYIDNMDEVKNGIYEVKYDGLEFKKSMNIIDLETFT
                     PITSKNSDVQNNSFKKVNMIIMLCSNYENIFNKYGLLAYRLLHLEAGHIAQNIQLVST
                     ALGIKSLPMGGWFDDKIETFINEISDKKNIYLYAIAVG"
     gene            1199..1363
                     /locus_tag="VQU28_002079"
     CDS             1199..1363
                     /locus_tag="VQU28_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477361.1"
                     /translation="MNAEDKFRELENENKLLKLHNKELENEIQNGWKFWIWVTVPILG
                     LIYAIIQKIF"
BASE COUNT          608 a          181 c          269 g          593 t
ORIGIN      
        1 ataactatta ttacgaaaaa aaattcttgt gtaaaattct attgactttc tatattattt
       61 ttgtatttca taacgaatgt aatttaacaa gtgaagaaat tttaaagatg gattcctatg
      121 ctattaattt ctgggagagt aaaggggtcg gtattgacaa aaaaatcttg gaatttattc
      181 aaaaaaatat tacagttatt aaaaataaaa attttttgaa taaagatttt gatatttatg
      241 ttaaaaagat tcataattta ttctttgtca tttctacttt agtttcaaaa gagcaataca
      301 tggggatagt ttcttcccaa atacatatgt tcgcaaatag gatgggaatt tctcccacca
      361 ttgaagttac catgctagat ttttttctga gggaggattc tgattaatat ggaaaaatat
      421 ttttttgata tttctatttt tgaaaaaata aaaaaagtat tatcatacca atctaaatat
      481 tgggaaatat cgaagcctag ggataagtac agtttcaaaa gtcaaaatag aatatctggc
      541 tctaatagca ttaaatttaa agattttgag ttagattatg aactggagtc acttggtaaa
      601 atacttttaa aaaggagatc gtttttacta aatgaatttg ataagaatgc gagttttgac
      661 aaactgattt acttgttgaa aactgtattc tctcctacaa attgcaagtt tgtatttgga
      721 gaagaggttc ctttatttag ctatcctaca tcaggtgcgt taaattcaat tactcctttt
      781 gtttatattg ataatatgga tgaagtaaag aatggtatat atgaagtaaa gtatgacgga
      841 cttgaattta aaaaaagtat gaatattata gacttagaaa catttacccc aataacaagt
      901 aaaaacagtg acgtacaaaa taattcattt aaaaaggtaa atatgataat tatgttatgt
      961 tcgaattatg aaaatatttt caataaatat ggattattag cttatcgact tttgcattta
     1021 gaggctggtc atattgcaca aaatatccag cttgtttcta ctgcgctagg aataaaaagt
     1081 ttaccgatgg gaggttggtt tgatgataaa atagaaactt ttattaatga aattagtgat
     1141 aaaaaaaata tttatctcta tgcaattgca gttggataaa tttaaggagg aagtgtagat
     1201 gaacgcagag gataaatttc gcgaactaga aaatgaaaat aaacttctaa aacttcataa
     1261 caaggagctt gagaatgaaa ttcaaaatgg atggaaattt tggatatggg taactgtccc
     1321 tattttaggt ttgatatatg ctattattca aaaaattttt taattatgct agtttgtcag
     1381 gttgagtagt ttttgttcaa aatttaataa acttttttca atgaagatta tctaaagatt
     1441 gcttctcaga tgagaatggc ttctctatct tatataaatt atcttcatag agtgtcctgt
     1501 tattcatgtt atcacagaat agatagtttt tttggctcta taatatttgt agtgggtaaa
     1561 tcccctatag atattatgga gcctattttt gtgtagaaaa aaagtcccat atcatctata
     1621 atgaaaagca accaaactat cattagaaag a
//
LOCUS       DAVBCB010000102         1586 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVBCB010000102 DAVBCB010000000
VERSION     DAVBCB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.102"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            84..491
                     /locus_tag="VQU28_002080"
     CDS             84..491
                     /locus_tag="VQU28_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HET2477362.1"
                     /translation="MARYGVYAVIPNPEQKQIVLVQEPNGAWFLPCGEIEAGENHQEA
                     LKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYYNPAYLYEATSFKEVQKPLENFN
                     HIAWFPIDEAIQNLKRGSHKWAIESWKKQHKIG"
     gene            656..955
                     /locus_tag="VQU28_002081"
     CDS             656..955
                     /locus_tag="VQU28_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HET2477363.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVNEANIKTLYSEKLIGISIPIK"
     gene            1216..1539
                     /gene="tnpB"
                     /locus_tag="VQU28_002082"
     CDS             1216..1539
                     /gene="tnpB"
                     /locus_tag="VQU28_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HET2477364.1"
                     /translation="MMIVLSDLGQVHLVCGKTDMRQGIDSLAYVVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWLLYKRFENGRLTWPSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
BASE COUNT          544 a          314 c          282 g          446 t
ORIGIN      
        1 actataataa taacgatact agataatgag gaaaatgcaa tggcaaaccc aactttcgga
       61 gaaaaaaagc gaatacagac tatattgctc gctatggcgt atatgcagtt atccctaatc
      121 ctgaacaaaa acaaattgtt cttgttcaag aaccaaatgg tgcttggttc ctaccatgtg
      181 gagaaattga agcaggtgaa aatcatcagg aagccctaaa gcgtgagttg attgaagagc
      241 ttggtttcac agcagaaatt ggtacctatt acggacaagc tgacgaatat ttctattctc
      301 gtcatcgtga tacctactac tacaatcctg cctacctcta tgaagcaact tctttcaaag
      361 aagtacaaaa gccactagaa aactttaatc atattgcctg gttccctatt gacgaggcta
      421 tccaaaacct taaacgtggt agccataaat gggccattga atcttggaaa aaacagcata
      481 agattggcta atactcttcg aaaataaaat tcaaaccacg tcagcgtcgc cttaccgtac
      541 tcaagtatag cctgcggcta gcttcctagt ttgctctttg attttcattg agtataaatc
      601 aagcgacttt attcaaaaag tgctataata gaattagaaa atcggaggaa atattatgaa
      661 gcttatcaat acgacaaact cacactcgca acttgtaaaa agtcaactag aaagtaccga
      721 tgcaaccctt gttgaggttt attctgcagg gaatacagat gttattttta cccaagctcc
      781 gcttcactat gaaatcctca tctcaaacaa acaccgtgct attcgtgagc ctgaaatcga
      841 aaccattcaa gaatttttct tgaaacgtaa aattgataag acctctgtta atgaagccaa
      901 tatcaagaca ctctactcag agaaattaat tggaatttca atcccaatca agtaagaaaa
      961 ttgagtaaaa actcgatttg agagaaagtc caataagtct ttccataaca aaacgcatag
     1021 taacaagagt ttctacaccc actattatac gtttttatca ttttgaaatc tgctgctaat
     1081 caatgaagaa gcaaagatca aactaggcag ctagttacaa gttacgtaag cgcgtcataa
     1141 caaggtatct atcctaattc cccaacttcc agtatactag ataaaaaata attatcagag
     1201 gaaaggtatt gtactatgat gattgtacta agtgatttag gtcaagttca ccttgtttgt
     1261 ggcaagacag atatgaggca gggaatagac tcattagcct atgtagttaa aacccacttt
     1321 gaattggatc ctttctccgg tcaagtcttt ctcttttgtg gtggacgtaa agaccgcttt
     1381 aaagcccttt actgggatgg tcaaggattt tggctactat ataaacgctt tgagaacggc
     1441 agactgactt ggcccagtac agaaaaggat gtcaaagctc tcacatctga acaagtagac
     1501 tggcttatga agggcttttc tatcactccc caaatatagt ggatttaaac tagaatagta
     1561 cacctctact tctaaaacat tgttag
//
LOCUS       DAVBCB010000103         1455 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVBCB010000103 DAVBCB010000000
VERSION     DAVBCB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.103"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(412..804)
                     /gene="rpsI"
                     /locus_tag="VQU28_002083"
     CDS             complement(412..804)
                     /gene="rpsI"
                     /locus_tag="VQU28_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HET2477365.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(824..1270)
                     /gene="rplM"
                     /locus_tag="VQU28_002084"
     CDS             complement(824..1270)
                     /gene="rplM"
                     /locus_tag="VQU28_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HET2477366.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
BASE COUNT          420 a          290 c          279 g          466 t
ORIGIN      
        1 cccaaactct tcacacaaga gttctaactt acccattcta tggaatcttg cattatccat
       61 aatatagtag attgaaacta gaatagtaca cctctacttc taaaacattg ttagaattcg
      121 atttgactat cctcggtttg tcctattctt atttcacctt actataaaaa taaggtaaac
      181 ttttcaaaaa aacacgaaaa aagcacccat agggcaaacc tttgagtgct ttgaaacatt
      241 gatataattg tattgattag cgttttgagg caactgacct tgtcaggttg cagccacatt
      301 tgtaagcaac taaagtcgca acaactgtgt caattgcacc aatttagtaa gaaagtataa
      361 aaaaagaaca ccccgaaagg tgctctgtat aagtatagta attctttcga attaacgttt
      421 actaaattgt gatgctttac gagctttctt aagacctggt ttcttacgtt caactttacg
      481 tgagtcacgt gtaagaagtc ctgcgcgttt caatgaatcg cggaagtctg ggtctacttg
      541 aagaagggca cgagcgatac cgtgacggat agctcctgat tgaccagcgt atccaccacc
      601 tacaacgtta acgaaaacgt cgtatgaacc tacagttgaa gtaactgcga atggttggtt
      661 gatgacaaga cgaaggtcag catgtgggat gtactcttca acatcttttt tgttaacagt
      721 gattttacca gttcctggaa caaggcgaac gcgtgcaaca gcgtttttac gacgtccagt
      781 acctgcatat tgtgcttgtg acatacttta tttgttcctt tccttagata agtcctgaaa
      841 tgtcaagaac ttctggttgt tgtgcagcat gagtgtgctc agctccaaca aatactttca
      901 acttcatacc ttgagcgcgt ccaagtgtat tgtgtggaag cataccttta actgatttct
      961 caatcaaacg tactgcattt ttagaacgaa gttcacctgc agagatttgt ttcaatccac
     1021 ctgggtggtt tgagtgagtg tagtagattt tatcagttgc ttttttacca gtcaatttaa
     1081 ctttttcagc attgataaca atcacgaagt cacctgtatc agtgtgtggt gtaaatgttg
     1141 gtttgttttt tccgcgaagt acgctagcaa ctactgcaga aagacgtcca agtggtacat
     1201 cagttgcgtc aactacgtac catttacgtt caacttggcc tggtttagcc ataaatgttg
     1261 ttttgttcat gatttctcct atatatagtt cgatttttgt ttacggtgat gggtgttcct
     1321 tcccatcgaa aagtatttgg aaggttccgg ggcctttcaa atggggtaaa caataccgcc
     1381 tactatcata ccaaatttca cccctaaaag tcaatagata tgaaaaatat ttttcggtaa
     1441 cttctcaaag taact
//
LOCUS       DAVBCB010000104         1389 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVBCB010000104 DAVBCB010000000
VERSION     DAVBCB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.104"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(704..1389)
                     /locus_tag="VQU28_002085"
                     /pseudo
     CDS             complement(704..1389)
                     /locus_tag="VQU28_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          386 a          283 c          287 g          433 t
ORIGIN      
        1 tacgtgacag agtccagttc tatcagaccg ttcgttttac agacacaagt gaagtaggat
       61 tacttcgatt agttgtttac cccttcgctc cacttccatt acagaagttt cctcactact
      121 atgggctcgg ctgacttctc atgattcgtt gttactacgc tttcggcgct catgagacct
      181 cacgggataa gccgtacatc tttcctcgtt tatacaagat atgaggacgt taaaaggtta
      241 caatgaaaat aggaaatctg acgcagagtc tttagactct aggaagattt atctttttca
      301 catagacctt agtccgaatt caattactct cgtgatttac gcatataggt tacgactacc
      361 tttttggact ttagagtttt aagccccctt atccgctgta tacgccttac tatcacgttc
      421 ctgttcgtag agcctcgatt tcgctatccc ttcctctgac gattcttatt tcctgcgtca
      481 gacttaaaac ggtctatccc cagaccgttt taatccgcta cctcacgata gtcaggcttg
      541 ggaatcgcta ttgggttcac cggtagcagg tgcccacgga ggacttacac ctcagatgca
      601 cgacatgccc gtcgtactta aaatcagcct aaatgattca ggctgatttt tctattgatt
      661 caaaaaaacg gctctttgtc aactgtagtg ggttgaagaa aagctaagat cgagaaagga
      721 caaatttcgt cctttctttt ttgatattca gagcgataaa aatccgtttt ttgaagtttt
      781 caaagttccg aaaaccaaag gcattgtgct tgataagttt gatgagatta ttggtcgctt
      841 ccaatttggc gtttgaatag ggtagttgaa gggcgttgat aatcttttct ttatctttga
      901 ggaaggtttt aaagacagtc tgaaaaatag gatgaacctg cttcagattg tcctcaataa
      961 gtccgaaaaa tttctctggt tccttattct ggaagtgaaa aagcaagagc tgatagagct
     1021 gatagtggtg tttcaagtct tccgaatagc tcaaaagctt gtctaaaatc tctttattgg
     1081 ttaaatgcat acgaaaagta ggacgataaa aacgcttatc actcagttta cggctatcct
     1141 gttgtatgag cttccagtag cgcttgatag ccttgtattc atgggatttt cgatgaaact
     1201 gattcatgat ttaaacacgc acacgactca tagcacggct aagatgttgc acaatgtgaa
     1261 agcgatcaag aacgatttta gcattcggga gtgaaacagt ctgggagact gtttcagcct
     1321 gagcctagaa atttgaaagc gaagctgtct agctaagtca tagtaagggc taaacatatc
     1381 catagtaat
//
LOCUS       DAVBCB010000105         1190 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVBCB010000105 DAVBCB010000000
VERSION     DAVBCB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.105"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            239..>1190
                     /locus_tag="VQU28_002086"
     CDS             239..>1190
                     /locus_tag="VQU28_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HET2477367.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFLLGG"
BASE COUNT          413 a          210 c          249 g          318 t
ORIGIN      
        1 agccacctta ccttagtcaa gaattagatg tttcactatg tttgagtaag ttgatgattt
       61 catttgataa caggtttgaa actgttaggc taggtggcca aggctaatca tagccttggt
      121 ttagctgaaa aaacaggttc aagggttcct gttgtcaaat gaaatgtgat ttaaggtata
      181 agaaaacacc tctgtgctat acttgttgtt caccacaaac acaaggaaag gcacagagat
      241 gcaagaacat tataccccaa aagggaaaca tttgacaata gataaccgtc gcttgattga
      301 gcggtggaag aatgaaaata agtccaatcg tgaaattgca ggcttgttag gaaaggcgcc
      361 tcaaacgatt cataatgaag tcaaaagagg tacaacttta caacaagtga gaaaagggct
      421 atacaaaaag gtctattctg ccgattacgc acaaactgtt taccaattca atcgaaaacg
      481 gtcggtgaaa aagttaattt taacaaagga aatcagagag aagatcttac actatcataa
      541 gcaaaaattt tcgcctgaaa tgatggttaa caagaagcaa gtgaaagttg gtatttcaac
      601 catctactac tggtttcata atggtcattt aggattgacg aaggccgaca tgctttatcc
      661 cagaaaaagg aaaggtgtca agaagcaagc tagtccgaac tttaagccgg caggtaaatc
      721 tatcgaagaa cgtcctgacg ttattaatct tcgcttggaa aatggtcatt atgaaattga
      781 taccgtccta ctgactaaga taaaaaatta ttgcctgtta gtcttaaccg accggcggag
      841 cagacaccaa attataaggt taattccaaa taaaactgct gaatctgtca atcaggcgct
      901 tacgttacta ttaggggagc atcgtattct gtccattact gcagataatg gttcggagtt
      961 caaacgattg tctgaggtat ttcctgagga acatatctac tacgcacatg cttactcttc
     1021 atgggagaga ggttcaaatg aaaatcataa tcgattaatt cggagatggt tacctaaagg
     1081 aaccaagaaa acgactccga aagaagtagc ttttatcgaa aattggatta acaactaccc
     1141 taaaaaatgc ttggactaca agtcgccaag tgaatttctt ttgggtggct
//
LOCUS       DAVBCB010000106         1142 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVBCB010000106 DAVBCB010000000
VERSION     DAVBCB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.106"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..1122
                     /gene="rlmD"
                     /locus_tag="VQU28_002087"
     CDS             <1..1122
                     /gene="rlmD"
                     /locus_tag="VQU28_002087"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HET2477368.1"
                     /translation="CGGCQIMHLHYDKQLEFKTDLLHQALKKFAPAGYENYEIRPTIG
                     MQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELKDCLVQDKETQVIANRLAELL
                     TYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNRQLNLTQLVKELVKDFPEVVT
                     VAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSLSPRAFYQLNPEQTEVLYSEA
                     VKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDIIPEAIEDAKRNAKRMGFDNT
                     HYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLLDTILTYVPEKMVYISCNVST
                     LARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKKV"
BASE COUNT          387 a          196 c          243 g          316 t
ORIGIN      
        1 tgcggaggct gccaaatcat gcacctgcat tatgataagc agctggagtt caagacggac
       61 ttacttcatc aagcgctgaa aaaatttgct cctgcaggat atgaaaatta tgaaattcgt
      121 ccaactattg gaatgcagga accaaaatat tacagagcta agttacaatt tcagactcga
      181 aaatttaaaa atcaggtcaa ggcgggctta tatgcacaaa actctcacta tttagtagag
      241 ttgaaagact gcctggtaca agataaggaa acccaagtga ttgctaatcg cttagcagaa
      301 ttacttactt atcaccagat tccaatcacg gatgagagaa aagttctagg tgtccgtact
      361 attatggtcc gacgcgcgag aaagaccgga caggttcaga ttattattgt tacaaaccgc
      421 cagcttaatt taactcaatt ggtaaaagag ttggttaaag atttcccaga agttgtgaca
      481 gtagctgtta atacaaatac agctaaaacc agtgagatat atggtgaaaa gacagagatt
      541 atctgggggc aagagagtat tcaagaaggt gtactcaatt atgaattttc actatcccct
      601 cgagcttttt atcaactaaa tcctgagcaa acagaagtcc tctatagcga agcagtaaaa
      661 gcgctggatg ttgataaaga agaccatttg attgacgctt attgtggagt tggaacgatt
      721 ggatttgcct ttgcaaagaa agtaaaaaca ctcagaggta tggatattat tccagaagct
      781 attgaagatg ccaagcgaaa tgctaaaaga atgggatttg acaatactca ttatgaagct
      841 ggaacggcag aagagattat tcctcgttgg tacaaggaag gctaccgagc agatgctttg
      901 attgttgacc caccacgtac aggtctggat gataagttat tagatactat tcttacttat
      961 gtaccagaaa aaatggttta tatttcttgt aatgtttcga ccttggctcg tgatttggta
     1021 cgcttagtag aagtctatga tcttcattat atccagtcgg tcgatatgtt cccacataca
     1081 gctcgaactg aagctgttgt aaaattaata aaaaaagttt aaaaaaggtg ttgacaaaac
     1141 at
//
LOCUS       DAVBCB010000107         1122 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVBCB010000107 DAVBCB010000000
VERSION     DAVBCB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1122
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.107"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..459)
                     /locus_tag="VQU28_002088"
                     /pseudo
     CDS             complement(1..459)
                     /locus_tag="VQU28_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
     gene            complement(695..1006)
                     /locus_tag="VQU28_002089"
     CDS             complement(695..1006)
                     /locus_tag="VQU28_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477369.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYVCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
BASE COUNT          348 a          236 c          186 g          352 t
ORIGIN      
        1 cgcatcatca taagaaacca cttcagcgac aaacttgtac tcagggattt tacgacgagc
       61 accaaacaac tgctcatttt cagatggtgt accatagtaa aatcctgtag ccagttcacg
      121 ttgggcaacc ttccacatct cgtccaccaa gtcttgtttg atagcttcaa acttttcagg
      181 actttcaaga taggcatcca caaccgcctt gtagcagttg gttactgtta atacatagtg
      241 aatagactcc atacgtcctt cgatttttag actgtccaca ccattttcaa tcatatctgg
      301 aatgtggtca atcatagaca tgtcaacggc tgacattgaa aattcttctg gaatctcacc
      361 ctgcaaactc ttacgttctt tcccaaatgg catatcgtaa aggtcgtatt tccaacggca
      421 tgactgagaa catccaccac ggttggcatc acgcatactc tagacgcaat tctaatcttt
      481 tagactttgc ataataactg ttaaacagtt gaaaattaac ccagttttaa caagtagtca
      541 aaataccaga taaaggaaaa aaagtcttta aaatcttaaa aacgcatagt atcaggtatt
      601 gaacaatctt gatattatgc gttttattgt gggaagattt acttcatttt ctcttggaat
      661 tgagttttct cctacttcaa aactagatga tttgctattt aaaatagcgt tccttatgat
      721 tttggcaacc tggattaaag cgagaaaaac aaaaggggca acttaagctt tctttatatt
      781 gcttatatgt tagtagtttt agacaaacac cacataaaat aggcttatcc tgtatcaaag
      841 ataagggata cggctcaaaa gggtgatttt ctaatgaatc atgacaccga taacaagcat
      901 agtacttttt acaaacatag cattgtaaag caataatatc cagcttgcta tggtaatgaa
      961 tgcatcttgt ttgcttatcc actaagaaac cctgtgcctc tatcatttcc ttttcctttc
     1021 ttaagctcga actgtgacgc tggttccatc ttccttataa agattgatga gtccttcttt
     1081 taatgcacga accatgtctc cagcttgaac aggttgaggc ac
//
LOCUS       DAVBCB010000108         1058 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVBCB010000108 DAVBCB010000000
VERSION     DAVBCB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.108"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..78)
                     /locus_tag="VQU28_002090"
                     /pseudo
     CDS             complement(1..78)
                     /locus_tag="VQU28_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(527..925)
                     /locus_tag="VQU28_002091"
     CDS             complement(527..925)
                     /locus_tag="VQU28_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HET2477370.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          321 a          199 c          158 g          380 t
ORIGIN      
        1 acgcttgatt tcagtgtgga tagtttgagg agcttttcca agtagagagg caatttctct
       61 atttgatttc cctttgttcg ccttttgtag tataatggtt ttgcatctct gtgcctttct
      121 tgtgtttgtg gttgaacaac aagtataaca caaaggtgtt ttcttatgcc tacaagagct
      181 ttcattattt ccattttctt ttggatttca ctctattctg aaaaacttgt gtatatttac
      241 tgaagctagc aagtcttacc tgtaaattta atgaaagcaa cacaaaatcc gagaggggaa
      301 tctcggatta atgtcaattt aaaaagatag agagttttta gtttaaataa attgttttaa
      361 aatatcaaca acatcacttc ttttcttaac ctgataagcc ttgattccta attttggggc
      421 tacgattata ttgtcctcaa tatcgtctag aaagacacaa tttctaggtt ataactggta
      481 tttatcgata gttactcata tatatcagtc cacctccata ctcaccttac agattctttt
      541 gataataata tctttgccta atgtagagat agtcttgcaa agaaaaaact tccttgtagc
      601 catgtttctg ataaaagtcc ggtgcctgga actggtaagt attgacaaag gcaaaacaac
      661 aatttcgatt cttagcttca ctttctgcct gttgcaatag ttttgaaccg attccttgcc
      721 ctcgcagttc ctcttttaca aacaaatact cgatttctag ccaatttcca aaagtctctg
      781 ctatcaaacc cgcaaggaga ttgccctttt catcttcgac ataaagatta agtggctcac
      841 tttcagcctc ttctcttttt gaacggttat aaacacgaat cagattccct atttcttgcg
      901 atttatgtga ttccttattt tccaatctaa agtataatga aatgaaataa aacatgcgca
      961 aatcgattaa ggaatttaat ctaatttcta acaatgtctt agaaatcaaa gtgtactatt
     1021 ttaacttcaa tgcactatac atctaatact caataaaa
//
LOCUS       DAVBCB010000109         1054 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVBCB010000109 DAVBCB010000000
VERSION     DAVBCB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.109"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..555
                     /locus_tag="VQU28_002092"
     CDS             <1..555
                     /locus_tag="VQU28_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2477371.1"
                     /translation="KDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSEATFWGTE
                     IPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKVAILDIPA
                     THNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSISELLIPIS
                     NHEEMKRIISKVDLLFQKVSQLFE"
     gene            complement(503..1048)
                     /locus_tag="VQU28_002093"
     CDS             complement(503..1048)
                     /locus_tag="VQU28_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001104691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2477372.1"
                     /translation="MNWVVIKIKDIFSINTGLSYKKGDLSINNKGVRIIRGGNIKPLE
                     FSLLDNDYYIDTQFISSEQVYLKHNQLITPVSTSLEHIGKFARIDKDYDGVVAGGFIF
                     QLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAPFE
                     EQELITQKVEKLFEKVNQLWK"
BASE COUNT          380 a          165 c          153 g          356 t
ORIGIN      
        1 aaagacgaaa caacctctta tcctatatat gaaatcccag aagcatggag atatattaaa
       61 tttgctagcc tagttaactt cagaatagga aaaactcctc cacgcagtga agctactttt
      121 tggggaactg aaataccttg ggtatctata tcagatatgc ctatatctgg ttatgtaact
      181 aatacgagag aatctatttc taaattagca ttaaaatcaa agaaaataga catttcacct
      241 aaaggaactt tattaatgag ttttaaatta tctattggaa aagttgccat attggatatt
      301 cctgccactc ataatgaagc cattatatcg atattcccct atgctaataa agaaaatatt
      361 atcagagatt acttaatgat atttttgcca cttatctcta ctttaggtga ttcaaaagat
      421 gctataaaag ggaaaacatt aaatagtacc agtatctccg aattattaat ccctatttct
      481 aatcatgaag agatgaaaag aatcatttcc aaagttgatt tactttttca aaaagtttct
      541 caactttttg agtaataagt tcctgttcct caaaaggagc taacggaatt aatagctcgc
      601 ttagtgtagt tttaggaata ttatataaag cttgacctga tagtttagtt attgctttca
      661 attgtttata aaataacgga gaggacaagt taaataatag aaattttgaa ataatctctg
      721 aactttcgaa tggtgttaat tggaaaataa atccaccagc cacaacacca tcatagtctt
      781 tatcgattct tgcaaacttt ccaatatgtt ctaaagaggt tgatacaggt gttattagct
      841 gattatgttt taaataaact tgctcagagg agatgaattg tgtatcaatg tagtaatcat
      901 tatccaacag agaaaattct aaaggcttaa tattaccacc acgtataatt ctaacacctt
      961 tattattaat gcttaaatcg cccttcttgt aagaaagacc tgtatttatt gaaaaaatat
     1021 cttttatttt tataacaacc caattcatag gtat
//
LOCUS       DAVBCB010000110         1048 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVBCB010000110 DAVBCB010000000
VERSION     DAVBCB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.110"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(238..325)
                     /locus_tag="VQU28_002094"
     tRNA            complement(238..325)
                     /locus_tag="VQU28_002094"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Ser,seq:gct)
     gene            complement(332..405)
                     /locus_tag="VQU28_002095"
     tRNA            complement(332..405)
                     /locus_tag="VQU28_002095"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(369..371),aa:Ile,seq:gat)
     gene            complement(441..511)
                     /locus_tag="VQU28_002096"
     tRNA            complement(441..511)
                     /locus_tag="VQU28_002096"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(477..479),aa:Gly,seq:tcc)
     gene            complement(531..603)
                     /locus_tag="VQU28_002097"
     tRNA            complement(531..603)
                     /locus_tag="VQU28_002097"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(568..570),aa:Phe,seq:gaa)
     gene            complement(607..680)
                     /locus_tag="VQU28_002098"
     tRNA            complement(607..680)
                     /locus_tag="VQU28_002098"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:Met,seq:cat)
     gene            complement(694..783)
                     /locus_tag="VQU28_002099"
     tRNA            complement(694..783)
                     /locus_tag="VQU28_002099"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(745..747),aa:Ser,seq:tga)
     gene            complement(866..939)
                     /locus_tag="VQU28_002100"
     tRNA            complement(866..939)
                     /locus_tag="VQU28_002100"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(903..905),aa:Ile,seq:gat)
     gene            complement(974..1044)
                     /locus_tag="VQU28_002101"
     tRNA            complement(974..1044)
                     /locus_tag="VQU28_002101"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1010..1012),aa:Gly,seq:tcc)
BASE COUNT          327 a          263 c          210 g          248 t
ORIGIN      
        1 gcaatgcctt tggttttcga aactttgaaa actttaaaaa acggattttt atcgctttga
       61 acatcaaaaa agaaaggacg aaatttgtcc tttctcgagc ttagcttttc ttcaacccac
      121 tacagttgac aaagagccat aaaaataact tcagattagt aattcaatta agttttacgg
      181 atctgaagct ttattgtctc taaaaataaa aaagagagtt acaaactctc attaaaacgg
      241 agaataaggg attcgaaccc ttgcgccagt tacccgacct aacgatttag caaaccgtcc
      301 tcttcagcct cttgagtaat tctccaatta atgggcacga gtggactcga accaccgacc
      361 tcacgcttat caggcgtgcg ctctaaccac ctgagctacg cgcccaagtt aaaaaacttg
      421 gtaatttgaa caaagttcaa agcgggtgac gagaatcgaa ctcgcgacaa cagcttggaa
      481 ggctgtagtt ttaccactaa actacacccg cataaatact atcaataaaa tggcgcgaga
      541 cggaatcgaa ccgccgacac atggagcttc aatccattgc tctaccaact gagctaccga
      601 gccttattgc gggagcagga tttgaaccta cgaccttcgg gttatgagcc cgacgagcta
      661 ccgagctgct ccatcccgcg ttaataatat aaaaggagga tgtgggattc gaacccacgc
      721 acgcttttac acgcctgacg gttttcaaga ccgttccctt cagccggact tgggtaatcc
      781 tccaaaatag tttatacggg aacaagcccg tgtcctctta gtgaacagat aatattccaa
      841 tcccctagta atggactaaa cactatgggc acgagtggac tcgaaccacc gacctcacgc
      901 ttatcaggcg tgcgctctaa ccacctgagc tacgcgccca agtcaaaaac ttggtacaaa
      961 gaacaaagtt caaagcgggt gacgagaatc gaactcgcga caacagcttg gaaggctgta
     1021 gttttaccac taaactacac ccgctaaa
//
LOCUS       DAVBCB010000111         1035 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVBCB010000111 DAVBCB010000000
VERSION     DAVBCB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.111"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQU28_002102"
     CDS             complement(<1..990)
                     /gene="ltrA"
                     /locus_tag="VQU28_002102"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HET2477373.1"
                     /translation="MSKLLDKILSRDNMLEAYNQVKSNKGSAGIDGITIEEMDGYLRQ
                     NWRLTKELIKQRKYKPQPVLRVEIPKPNGGIRQLGIPTVMDRMIQQAIVQVISPICEP
                     HFSDTSYGFRPNRSCEKAIMKLLEYLNDGYEWIVDIDLEKFFDTVPQDRLMSLVHNII
                     EDGDTESLIRKYLHSGVIINGQRYKTLVGTPQGGNLSPLLSNIMLNELDKELEKRGLR
                     FVRYADDCVITVGSEAAAKRVMYSVSRFIEKRLGLKVNMTKTKITRPRELKYLGFGFW
                     KSSDGWKSRPHQDSVWRFKLKLKKLTQRKWSIDLTRRIEQLNLSIRGWINYFSL"
BASE COUNT          270 a          226 c          183 g          356 t
ORIGIN      
        1 caatgagaaa tagtttatcc atcctcgaat agacaaattc agttgctcaa tacgtcttgt
       61 taggtctata ctccatttcc tctgtgttag tttcttcaat ttaagcttaa atctccaaac
      121 actatcttga tgtggacgac ttttccaacc atctgatgat ttccagaacc cgaaacctag
      181 atatttcaac tctcttggtc tggtaatctt agtcttggtc atgtttactt tcagacctag
      241 ccgtttctca ataaaacgac tgactgaata catcacacgc ttagcggctg cctcgcttcc
      301 gaccgtaatc acacaatcgt ctgcgtagcg aacaaatcga agccctctct tttctaattc
      361 cttgtccaat tcattaagca tgatattgga taagagagga gataaatttc ctccctgtgg
      421 cgtgccaact agcgttttat aacgttgacc gttaatgata acacctgaat gaagatattt
      481 acgaatcaag gattccgtat ctccgtcttc gataatgtta tgtactaagg acatcaatct
      541 atcttgagga actgtatcga aaaatttctc taggtctatg tccactatcc actcatagcc
      601 gtcatttaag tactctaaga gcttcatgat ggctttttca catgacctat ttggtctgaa
      661 tccatagctc gtatctgaga aatggggttc acaaataggg ctaataactt gaacaatggc
      721 ctgttgaatc attctatcca taactgttgg aattcctagt tggcggatgc ctccgtttgg
      781 tttgggaatc tcaactctaa gaactggttg aggcttatat tttctctgtt ttatcagttc
      841 cttagtcagg cgccagtttt gtctgagata gccatccatc tcttcgatag taattccatc
      901 aatcccagct gagcctttat tagattttac ttgattgtag gcttcaagca tattgtcgcg
      961 tgataatatt ttatctagca attttgacat gtgcctattc ctttcttgtt ttggtgcctg
     1021 agggacatct tatat
//
LOCUS       DAVBCB010000112          978 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVBCB010000112 DAVBCB010000000
VERSION     DAVBCB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.112"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..255)
                     /locus_tag="VQU28_002103"
                     /pseudo
     CDS             complement(1..255)
                     /locus_tag="VQU28_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            524..978
                     /locus_tag="VQU28_002104"
                     /pseudo
     CDS             524..978
                     /locus_tag="VQU28_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          324 a          187 c          186 g          281 t
ORIGIN      
        1 ctcttctaaa aaggcgttca tccactcagt attacatcca ccagctatta agaaaaatga
       61 ttcgcctgta tgggcatcaa cagctccata acaatagtga aattctcgta tatagtgact
      121 atggacatgt ggacctactc ctattggaga ccaacaagat cccagtttac tgattctacc
      181 gaagccagcc tcatcttggt acatcaagcg aaccttatga aaacgtctac tggttttaaa
      241 gcgctttctt ttcttcagtt gatttcatat cactattgta taatgctttt tgattttagt
      301 ctagtataaa tagcgagaaa tatctatcct atcttctaga atgtcttcca aacgaggaaa
      361 ctctcgtaaa caaagaggtt ttagaggcct atttaccgtg gactaaagtt gcacaagaaa
      421 agtgcaaata agaaatctcc agattaggaa ctatccgtga gttctctagt ctggagattt
      481 ttcaatagac ttcctgcgaa acaaaatatg gtataatagt tctatgaatt atgaagcaag
      541 taaacaacta accgatgcac gatttaagcg tcttgttggt gttcaacgca cgacttttga
      601 agagatgtta gctgtattaa aaacagctta tcaacttaaa cacgcaaaag gtggacgaaa
      661 acctaaatta agcctagaag accttcttat ggccactctt caatatgtgc gagaatatcg
      721 aacttatgaa gaaattgcgg ctgatcttgg tattcacgaa agcaacttac tccgtcggag
      781 ccaatgggtt gaagtaactc ttgttcaaag gtggtgttgc gatttcaaga actcctctca
      841 gttctgagga cacggtaatg attgatgcga cggaagtaaa aatcaatcga cctaaaaaaa
      901 gaattagcga attattctgg taaaaagaaa tttcacgcta tgaagactca agcgattgtc
      961 acaagtcaag ggagaatt
//
LOCUS       DAVBCB010000113          938 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVBCB010000113 DAVBCB010000000
VERSION     DAVBCB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.113"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            1..938
                     /locus_tag="VQU28_002105"
                     /pseudo
     CDS             1..938
                     /locus_tag="VQU28_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          291 a          209 c          155 g          283 t
ORIGIN      
        1 agatacacac aaagaaatca tcgccaaact ggactacgac gccccatctt gccctgagtg
       61 cggaagtcaa atgaagaaat atgactttca aagaccttct aaaattcctt atcttgaaac
      121 aactggtatg ccttctagaa ttctccttag aaaacgccgt ttcaagtgct atcactgttc
      181 aaaaatgatg gttgctgaga ctcccctggt aaagaaaaat caccaaatcc ctcgtatcat
      241 caaccaaaaa attgctcaaa agttaattga aaaaatttct atgactgata ttgctcatca
      301 gctggccatt tcaacttcaa ctgtcattcg caagctcaat gactttcact ttgagcatga
      361 tttttcgcgt cttcctgaga ttatgtcttg ggatgagtat gcttttacaa agggaaagat
      421 gagcttcatt gcgcaagatt ttaacaatct caatatcatc actgttcttg agggaagaac
      481 acaagctatc attcgaaatc actttcttaa atatgataga gccgtccgat gtcgcgtcaa
      541 aatcattact atggatatgt ttagccctta ctatgacttg gctaaacagc ttcgctttca
      601 aatttctagg ctcaggctga aacagtctcc cagactgttt cactcccgaa tgctaaaatc
      661 gttcttgatc gctttcatat tgtacaacat cttagccgtg ctatgagtcg tgtgcgtgtc
      721 caaatcatga atcagtttca tcgaaaatcc catgaataca aggctatcaa gcgctactgg
      781 aaactcattc aacaggatag ccgtaaactg agtgataaac gtttttatcg tcctactttt
      841 cgtatgcact taaccaataa agagatttta aacaagcttt tgagttattc ggaagacttg
      901 aaacaccact atcaactcta tcaactcttg ctttttca
//
LOCUS       DAVBCB010000114          922 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVBCB010000114 DAVBCB010000000
VERSION     DAVBCB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..922
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.114"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            122..590
                     /locus_tag="VQU28_002106"
                     /pseudo
     CDS             122..590
                     /locus_tag="VQU28_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          336 a          139 c          172 g          275 t
ORIGIN      
        1 gagaagagga ttgccatctt ctccattttt atgtgcagag ttatagtggc ttgatgctgg
       61 gaaagtacac tgtgactgct aaaacattcc tagaagctgc tttgatttcc ctaatctatt
      121 tatgcaaatt ttatgttatt ttactataac agttgttgct aagccaaata atagtgggga
      181 agttcattta gacgtaagca ttgaagataa tcagggaggt agtgggtata atttcagttc
      241 tgtttcaagt agctcacaaa cagctaaata tgaaggaact ggttataata acaattcatc
      301 attatatata acgattgata aaacgtctga tgcaacagct cttttgaaat taaagttgaa
      361 taatgttgat aatcaacctg ctactgaagt tcctagttca ggaattactg taaaattaaa
      421 tgctaaagat aatgctggaa actggacaag tgcttcgaat aaagacaagt gcttcgaata
      481 aaaaagaaat aacagtaaaa attgtttctg ctaaaccgac atatccagac aaaatcttag
      541 tgaaaaatcc tgataatata aaagatacag aaaaaaatgc cattattgaa aaattgaaag
      601 aggcaaataa aaatcatcca gcaggagctc caacctttgc taaaggtgaa ggagagcatg
      661 caaatgatat tgtagcaact tattcagatg gtacaactta ttatgtaccg ttaaatgatg
      721 tgacaaaata tgcgaggtag tggctgtacc actcacttat tcacctcccc gtgatttgta
      781 gtagtgatag gctttctcac tattattata aaacaaaata aagatcacaa cactttttca
      841 ttctgtgttg tgccttgagt gaaacgaaag gaatgaatta taaatatgaa aagtatagtc
      901 actagcatag atgagcgctt gc
//
LOCUS       DAVBCB010000115          830 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVBCB010000115 DAVBCB010000000
VERSION     DAVBCB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.115"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            21..830
                     /locus_tag="VQU28_002107"
                     /pseudo
     CDS             21..830
                     /locus_tag="VQU28_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558050.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1515 family transposase"
BASE COUNT          266 a          174 c          162 g          228 t
ORIGIN      
        1 aaaagtgtta tactattttt atggaaacag tatacgacaa agcacaaaaa cttaactcaa
       61 aaaacttcaa actattgatt ggtgtcaaaa aggaaacctt tcaactcatg ctagaacacc
      121 tgaattcagc ctatcagatt cagcaccgaa aaggtggacg tccacgtagt ctgcccatgg
      181 aagaccagct cattatgacc ctccgttact tgcgatatta tcccactcag cgtctgctgg
      241 cctttgattt tggcgtcggt gtagctacgg taaatgccat catcacttgg gtggaggata
      301 cacttcgtgc gtcaggtagc tttgatttgg accatttaga agccccgagt gctgctgtgg
      361 ctattgacgt gaccgaaagt ccgattcagc gtccaaaaaa aaccaaagca aaaattattc
      421 tggtaaaaag aaacgacaca ccttaaaaac tcaaattatg ctggatttga cgacacataa
      481 agtctgtcaa atggcctttt ctgacggaca tacgcatgat tttactctct tcaaagaaag
      541 tattggacaa agtttgcctg aaacgacgct tgcctttgtt gacctaggtt atttaggcat
      601 cttgaaattt catgagaata ctttcattcc tgctaaaaat tccaaaaatc gccgcctgag
      661 tgaggatgat aagcagttaa ataaagagat gtcagcgata cgaattgaaa ttgaacattt
      721 taacgctaaa ttcaagacct tccaaatcat gtcagtccct tatcgtaacc gcagaaaacg
      781 tttcgagtta cgggcggaat taatttgtgc catcatcaat tatgaagtga
//
LOCUS       DAVBCB010000116          810 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVBCB010000116 DAVBCB010000000
VERSION     DAVBCB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..810
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.116"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(144..731)
                     /locus_tag="VQU28_002108"
     CDS             complement(144..731)
                     /locus_tag="VQU28_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HET2477374.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            complement(734..>810)
                     /locus_tag="VQU28_002109"
     CDS             complement(734..>810)
                     /locus_tag="VQU28_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HET2477375.1"
                     /translation="MPQSPWVERIVQVLDLDYEDLFRR"
BASE COUNT          298 a          167 c          111 g          234 t
ORIGIN      
        1 tctctccacc agtaaaatgc aagcaattat acaatgttgt cacatagaaa ataatgtttc
       61 cgtaactttt caaagtaact tccatctctc tcccaaaact ggaagttagt tttagaagtt
      121 acctaaaaat caggtcacct attttaaaaa agcagcaaac tataaactag taggttccac
      181 accaaatgta gtcccatact gccccataag tcagatttat agcgcaccat acctaaaaac
      241 atcccaagtg aaacatacaa acaccaagct agaatggttc ctgtatgatg tgctaaggca
      301 aataaaacac ttgtcaaagc aactctgata tctaattttc tgaccaaatt ccataaaatt
      361 tctcgataca gaaattcttc aaccatactc gcattgatta agaacaataa aaatgaaaac
      421 caaggaattt gatgttgaag gccaattaag tttgcttgat tcgtgcttcc ttgagcatga
      481 atcagactaa aacatagact tataatcagt aggctaacaa attcaacacc aagccatttc
      541 atcctagatt tcatattgac cttatgcgct tgtttgcgtt ggccatacat ccataaaaaa
      601 gaaatgagtg acgaaccata gagaatctgt agtatagtta actcaccgat acaaagaaat
      661 ttcaataagt atagagatac caataggaca tttacttgtt ggaatatata aactggaatt
      721 attcttttca tagttacctc cgaaataaat cttcataatc taaatctaat acctgcacaa
      781 tcctttctac ccatggactt tgaggcattc
//
LOCUS       DAVBCB010000117          738 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVBCB010000117 DAVBCB010000000
VERSION     DAVBCB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.117"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>738
                     /locus_tag="VQU28_002110"
     CDS             <1..>738
                     /locus_tag="VQU28_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ISL3-like element IS1167A family transposase"
                     /protein_id="HET2477376.1"
                     /translation="IEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSWDE
                     YAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRYDRAVRCQVKIITMDMFSPY
                     YDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQFERKSHEYKAIKRYWKLIQQ
                     DSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKHHYQIYQLLLFHFQNKDPEKFF
                     GLIEDNLKQVHPLFQTVFKTFLKNKEK"
BASE COUNT          227 a          151 c          122 g          238 t
ORIGIN      
        1 aattgaaaaa atttctatga ctgatattgc ccatcagctg gccatttcaa cttcaactgt
       61 cattcgcaag ctcaatgact ttcactttga gcatgatttt tctcggcttc caaagattat
      121 gtcttgggat gagtatgcct tcactaaggg aaagatgagt ttcattgcgc aagattttga
      181 taatcttaat attatcactg ttcttgaagg cagaacacaa gctgtcatcc gaaatcactt
      241 tcttcgctac gatagagccg ttcgttgtca agtgaaaatc attacgatgg atatgtttag
      301 tccttactat gacttggcta aacagctttt tccgtgtgct aaaatcgttc tagatcgttt
      361 ccatattatc caacatctca gccgtgccat gagtcgtttt cgtgttcaaa ttatgaatca
      421 gtttgaacga aaatctcatg aatacaaggc tatcaagcgt tactggaaac tcatccaaca
      481 ggatagtcgt aaattgagcg ataaacgttt ttatcgccct acttttcgca tgcacttaac
      541 aaataaagaa attcttgaca agattttaag ctattcagaa gacttgaaac accactatca
      601 gatctatcaa ctcttacttt ttcactttca gaacaaagac cctgagaaat ttttcggact
      661 cattgaggac aatctgaagc aggttcatcc tctttttcag actgtcttta aaacctttct
      721 aaagaacaaa gagaaaat
//
LOCUS       DAVBCB010000118          650 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVBCB010000118 DAVBCB010000000
VERSION     DAVBCB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.118"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            101..265
                     /locus_tag="VQU28_002111"
     CDS             101..265
                     /locus_tag="VQU28_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477377.1"
                     /translation="MKKKILIIFVLYLIMSIFLYPLRESAWYQLFYTIAYVIAVMIYF
                     AINKKKGEKK"
     gene            262..>650
                     /locus_tag="VQU28_002112"
     CDS             262..>650
                     /locus_tag="VQU28_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HET2477378.1"
                     /translation="MKTFFAKKRNIFLARLFLGQLPLLVSTYLFLSRQFLNFSVVFQF
                     LLVVINLASILVTVYLTREMRIREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITA
                     GESYQQIIAYIGAVLCLIIMLLLMWGL"
BASE COUNT          184 a          103 c          119 g          244 t
ORIGIN      
        1 atttatgatt tgagtgatcc aaggtcaaaa taagtgctat actagcagtg taaatgttct
       61 tgctcaacta agataaatcg cacatttagg aggaaatctt atgaagaaaa aaatactgat
      121 tattttcgtt ctgtatctaa tcatgtccat ctttctatat ccgcttaggg agagtgcttg
      181 gtatcagcta ttttatacca tagcctatgt gattgcagtt atgatctatt ttgctatcaa
      241 taaaaagaaa ggagaaaaga aatgaaaact ttttttgcta aaaaacggaa catcttcctt
      301 gcgagattgt tcttaggtca gttgcccttg cttgtttcta cttatctatt tctatctcgt
      361 cagtttttaa atttttccgt agttttccaa tttcttttag tagttattaa cttggcttct
      421 attttggtta ctgtttatct cactagggaa atgaggataa gagagtttga agatgatgat
      481 ttggttagtc ctagaaccaa tcaacttatg ttcatcggct tgacaggttt tatgtctatt
      541 atttgtttgt atagaggtat cacagcagga gaatcctacc aacaaataat cgcttatatt
      601 ggcgctgttc tctgcttaat catcatgctt ctgctcatgt ggggcttgaa
//
LOCUS       DAVBCB010000119          623 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVBCB010000119 DAVBCB010000000
VERSION     DAVBCB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.119"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..223
                     /locus_tag="VQU28_002113"
     CDS             <1..223
                     /locus_tag="VQU28_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2477379.1"
                     /translation="QAQTPRKSSKLKPLTAEDKACNHVLSKERSKVENIFAKVKTLKM
                     FSTTYRNHRKRFGLRMNLIVGIINHELGF"
     gene            240..623
                     /locus_tag="VQU28_002114"
                     /pseudo
     CDS             240..623
                     /locus_tag="VQU28_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT          236 a           95 c          111 g          181 t
ORIGIN      
        1 tcaagcacaa actccacgta aatccagcaa actcaagccg ctaacagctg aagataaagc
       61 ctgtaaccat gtgctatcta aggagagaag caaggttgag aacatctttg ccaaagtaaa
      121 aacgcttaaa atgttttcaa caacctatcg aaatcatcgt aaacgcttcg gattacggat
      181 gaatttgatt gttggtatta tcaatcatga actaggattc tagttttgca ggaagtctat
      241 tatcaaaaat accatcagga ttatataaga ttgatacagg aaaagtttta ttttatggta
      301 taaatattaa tcaaatagat aaaaaaatat taagtcaaaa tttaggagta gttccacagg
      361 attcattttt attgaaccga agtattcttg ataatataac tttaaagcac gaagttactt
      421 cacaaaagat agaggaagtt tgtaaagcag ttcaaatcta tgatgaaatc atggctatgc
      481 cgatgaaatt taatactatc atctcagaga tggggtcaaa tatttcaggt gggcaaaggc
      541 aacagatagc actggcacgt gcattaataa ataatcctag tattgtaatt ttagatgaag
      601 caactagtgc attagacact att
//
LOCUS       DAVBCB010000120          611 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVBCB010000120 DAVBCB010000000
VERSION     DAVBCB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.120"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(189..>611)
                     /locus_tag="VQU28_002115"
     CDS             complement(189..>611)
                     /locus_tag="VQU28_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2477380.1"
                     /translation="KPNEEKPQTEKPEEDKEHDEVSEPTHPESDEKENHAGLNPSADN
                     LYKPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAVET
                     LTGLKSSLLLGTKDNNTISAEVDSLLALLKESQPTPIQ"
BASE COUNT          155 a          121 c           93 g          242 t
ORIGIN      
        1 gatgaagttt tttctcaact tcttttactt tattaaatag ttttctaaac cggttattta
       61 accatttaat taaccagtaa atagtttact atttaaaatt acttttgtca agtttctgtt
      121 acgtttttct aaaaaagaga agtaacacga aaggttactt ctccataaaa tatgctccat
      181 tcattttact actgtatagg agtcggttga ctttctttta acaaagccaa gagactatct
      241 acttctgctg aaatagtgtt attatctttc gttccgagaa gaagactact ttttagacca
      301 gtcaatgtct ccacagcatt ttgtctaata ctagaatctg ttactttttc tagcaaggcc
      361 tccgcctctg ctatcttagc gttaataaca gaatgctcta cttgaggaat ttcagcctca
      421 tctgtagtat cttcagcttc ttcctctgtc tcttccgtat cagtgcttgg tttataaaga
      481 ttatctgctg aaggatttaa accagcgtga ttctcttttt catcagattc agggtgagtt
      541 ggctcactta cttcatcatg ttccttatct tcctcaggtt tttctgtctg aggtttctcc
      601 tcgtttggtt t
//
LOCUS       DAVBCB010000121          569 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVBCB010000121 DAVBCB010000000
VERSION     DAVBCB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.121"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>569)
                     /locus_tag="VQU28_002116"
     CDS             complement(<1..>569)
                     /locus_tag="VQU28_002116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477381.1"
                     /translation="AEALVDAEADALVEADSEAEVLAEADALVDAEADALVLAEADAL
                     VDAEADALVLAEAEALVDAEADALVLAEAEALVDAEADALVLAEADALVLAEAEALVD
                     AEAEALVDADSEALVDAEADALIDADSEADVLAEAEALVDAEAEALVDADSEALVDAE
                     AEADVDAEADALVDAEADALVDAEAEALV"
BASE COUNT          137 a          187 c          130 g          115 t
ORIGIN      
        1 tcaaccagcg cctcagcttc agcatcaacg agtgcgtcgg cttcagcatc aacgagtgca
       61 tcggcttcag catcaacgtc tgcctcagct tcagcgtcaa ccagtgcctc tgaatcggca
      121 tcaaccagtg cttcagcctc agcatcgaca agcgcttcgg cttcagcaag tacatcagct
      181 tctgaatctg catcaataag tgcgtcggct tcagcgtcaa ccagtgcctc tgaatcggca
      241 tcaaccagtg cttcagcctc agcatcaacg agtgcttcgg cttcagcaag tacaagtgcg
      301 tcagcttcag caagcacaag tgcgtcagct tcagcatcaa cgagcgcctc ggcttcagca
      361 agcaccagtg cgtcagcttc agcatcaacg agtgcctcgg cctcagcaag tacaagtgcg
      421 tcagcctcag catcaaccag tgcatcagcc tcagcaagta ccagtgcatc agcttccgca
      481 tcaacgagtg catcagcctc agcaagcacc tcagcttctg aatcggcctc aacgagtgcg
      541 tcagcctcag cgtcaacgag tgcttcggc
//
LOCUS       DAVBCB010000122          482 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVBCB010000122 DAVBCB010000000
VERSION     DAVBCB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.122"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>482)
                     /locus_tag="VQU28_002117"
     CDS             complement(<1..>482)
                     /locus_tag="VQU28_002117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477382.1"
                     /translation="EALVLAEADALVLAETEALVLAEAEALVDAEAEALVLAEAEALV
                     DADSDAEVLAEAEALVDAEAEALVLAEAEALVLADSDALVDAEAEALVDAEAEALVDA
                     EADALVDAEAEALVDAEAEALVDAEAEALVLAEADALVDAEAEALVLAEADALVEAEA
                     "
BASE COUNT          120 a          161 c          106 g           95 t
ORIGIN      
        1 tcagcttcgg cctcaacaag tgcgtcagct tcagcaagca ccagtgcttc ggcttcagcg
       61 tcgacaagtg cgtcagcctc agcaagcacc agtgcctcag cttcagcatc aacaagtgct
      121 tcagcctcag cgtcaacaag tgcttcagct tcagcatcaa caagtgcgtc agcttcagca
      181 tcaaccagtg cctcagcctc agcgtcgaca agtgcctcgg cttcagcatc gacaagtgca
      241 tctgaatcag caagcacaag tgcttcagct tcagcaagta ccagcgcctc agcctcagca
      301 tcaaccagtg cttcggcttc agcaagtacc tcagcgtctg aatcggcatc aacaagtgct
      361 tcagcttcag caagtacaag cgcctcagct tcagcatcaa ccagtgcttc ggcttcagca
      421 agcaccagtg cctcagtctc agcaagcacc agtgcgtcgg cttcagcaag cacaagcgcc
      481 tc
//
LOCUS       DAVBCB010000123          463 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVBCB010000123 DAVBCB010000000
VERSION     DAVBCB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.123"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(135..208)
                     /locus_tag="VQU28_002118"
     tRNA            complement(135..208)
                     /locus_tag="VQU28_002118"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(172..174),aa:Asn,seq:gtt)
     gene            complement(214..329)
                     /gene="rrf"
                     /locus_tag="VQU28_002119"
     rRNA            complement(214..329)
                     /gene="rrf"
                     /locus_tag="VQU28_002119"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          140 a          108 c           74 g          141 t
ORIGIN      
        1 tttacgaaaa tctattgaat atgccataaa aagattatac cacattgtgt actatttttg
       61 gttcatttta ctataacacg aaatagatta ttattacata acaaaaaaga ggtctaaacc
      121 tcttaactaa attactccgc cagtaggact cgaacctacg acatcatgat taacagtcat
      181 gcgctactac caactgagct atggcggatt aaagctaagc gacttcccta tctcacaggg
      241 ggcaaccccc aactacttcc ggcgttctag ggcttaactt ctgtgttcgg catgggtaca
      301 ggtgtatctc ctaggctatc gtcacttaac tctgagtaat acctactcaa aattgaatat
      361 ctattcaatt caagaaaacc gttcgctttc atattctcag ttactttgga taagtcctcg
      421 agctattagt attagtccgc tacatgtgtc gccacacttc cac
//
LOCUS       DAVBCB010000124          453 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVBCB010000124 DAVBCB010000000
VERSION     DAVBCB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..453
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.124"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            57..>453
                     /locus_tag="VQU28_002120"
     CDS             57..>453
                     /locus_tag="VQU28_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HET2477383.1"
                     /translation="MNDEASKQLTDARFKRLVSVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLSLEDLLMATLHYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGFTISR
                     TPLSSEDTVMIDATEVQINRPKKRISELFW"
BASE COUNT          152 a           85 c           90 g          126 t
ORIGIN      
        1 tccagtattt gacaatatta gacttcctgc gaaacaaaac atggtatagt agttctatga
       61 atgatgaagc aagtaaacaa ctaaccgatg cacgatttaa gcgtcttgtt agtgttcagc
      121 gtaccacttt tgaagagatg ttagctgtat taaaaacagc ttatcaactt aaacacgcaa
      181 aaggtggacg aaaacctaaa ttaagcctag aagaccttct tatggccact cttcactatg
      241 tgcgagaata tcgaacttat gaacaaattg cggctgattt tggtatccac gaaagcaact
      301 taatccgtcg gagccaatgg gttgaagtaa ctcttgttca aagtggtttt acgatttcaa
      361 gaactcctct cagttctgag gacacggtaa tgattgatgc gacggaagta caaatcaatc
      421 gccctaaaaa aagaattagc gaattattct ggt
//
LOCUS       DAVBCB010000125          430 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVBCB010000125 DAVBCB010000000
VERSION     DAVBCB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..430
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.125"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            321..>430
                     /locus_tag="VQU28_002121"
     CDS             321..>430
                     /locus_tag="VQU28_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIBC"
                     /protein_id="HET2477384.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVATLLEEREIV"
BASE COUNT          152 a           59 c           78 g          141 t
ORIGIN      
        1 ctaatctgga gatttttcaa tagacttcgt tattgggcgg ttacgatatg atcactgact
       61 tcgtcagtct tatctacaac ctcaaaactg tgttttgagc aacctgcggc tagcttccta
      121 gtttgctctt tgattttcat tgagtattag aacagaaaaa atacttggag tatttgtttg
      181 tgtgtttatt tttatataac aaactataaa caaaacaaaa atataaaaaa agagacaaaa
      241 aagaacagaa agcaattgac aacggtttca tatagtgtta tacttatggc ataaagttaa
      301 tggaaagaag ggaaaaactt atggtattag attatttctt tgacaaaaac cttgtgtttt
      361 gcttagaagc ggataatcaa gaacaactct ttgatcaggt tgcaacttta ttggaagaac
      421 gagaaattgt
//
LOCUS       DAVBCB010000126          393 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVBCB010000126 DAVBCB010000000
VERSION     DAVBCB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..393
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.126"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>393
                     /locus_tag="VQU28_002122"
     CDS             <1..>393
                     /locus_tag="VQU28_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HET2477385.1"
                     /translation="YLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFR
                     NRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSK
                     EKQEELERVLSNERFRGRQRVLRDLRIVF"
BASE COUNT          143 a           57 c           93 g          100 t
ORIGIN      
        1 taccttcatt tatgggaatt gtcggattgg gaccaagaag gcatgctctg cttacatgaa
       61 ttgattagta gagaagaagg actggtagac gatattccac gtttaaggaa atatttcaaa
      121 accaagtttc gaaatcgaat tttagactat atccgtaagc aggaaagtca gaagcgtaga
      181 tacgataaag aaccctatga agaagtggga gagatcagtc atcgtataag tgaggggggt
      241 ctctggctag atgattatta tctctttcat gaaacactaa gagattatag aaacaaacaa
      301 agtaaagaga aacaagaaga actagaacgc gtcttaagca atgaacgatt tcgagggcgt
      361 caaagagtat taagagactt acgcattgtg ttt
//
LOCUS       DAVBCB010000127          311 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVBCB010000127 DAVBCB010000000
VERSION     DAVBCB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.127"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>311)
                     /locus_tag="VQU28_002123"
     CDS             complement(<1..>311)
                     /locus_tag="VQU28_002123"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HET2477386.1"
                     /translation="ALVLAEADALVDAEAEALVDAEAEALVDADSEADVLAEADALVD
                     ADSDALVDAEAEALVDADAEALVLAEADALVLAEAEALVDAEAEALVLAEADALVEAE
                     A"
BASE COUNT           77 a           99 c           71 g           64 t
ORIGIN      
        1 tcagcttcgg cctcaacaag tgcgtcagct tcagcaagca ccagtgcttc ggcttcagcg
       61 tcgacaagtg cctcagcttc agcaagtacc agtgcgtcag cctcagcaag tactagcgcc
      121 tcagcatcag cgtcaacaag tgcttcggct tcagcgtcaa cgagtgcgtc tgagtcagca
      181 tcaacgagtg cgtcagcctc agcaagcaca tcagcttctg aatccgcatc aacaagtgct
      241 tcagcctcag cgtcaacaag tgcttcagct tcagcatcaa ccagtgcatc agcttcagca
      301 agtactagcg c
//
LOCUS       DAVBCB010000128          292 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVBCB010000128 DAVBCB010000000
VERSION     DAVBCB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.128"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            51..>292
                     /locus_tag="VQU28_002124"
     rRNA            51..>292
                     /locus_tag="VQU28_002124"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           90 a           54 c           81 g           67 t
ORIGIN      
        1 gaaaaaaaca ataaatctgt cagtgacaga aatgagtgag aactcaaact tttaatgaga
       61 gtttgatcct ggctcaggac gaacgctggc ggcgtgccta atacatgcaa gtagaacgct
      121 gaaggaggag cttgcttctc tggatgagtt gcgaacgggt gagtaacgcg taggtaacct
      181 gcctggtagc gggggataac tattggaaac gatagctaat accgcataag agtagatgtt
      241 gcatgacatt tgcttaaaag gtgcacttgc atcactacca gatggacctg cg
//
LOCUS       DAVBCB010000129          288 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVBCB010000129 DAVBCB010000000
VERSION     DAVBCB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.129"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>288
                     /locus_tag="VQU28_002125"
     CDS             <1..>288
                     /locus_tag="VQU28_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HET2477387.1"
                     /translation="NRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPHYDHYHNIKFEW
                     FDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGFGNASDHVQRNKNG"
BASE COUNT           93 a           59 c           59 g           77 t
ORIGIN      
        1 caaccgcgtg aaagcagcta agaaggtgcc acttgatcgt atgccttaca atcttcaata
       61 tactgtagaa gtcaaaaacg gtagtttaat catacctcat tatgaccatt accataacat
      121 caaatttgag tggtttgacg aaggccttta tgaggcacct aaggggtata ctcttgagga
      181 tcttttggcg actgtcaagt actatgtcga acatccaaac gaacgtccgc attcagataa
      241 tggttttggt aacgctagcg accatgttca aagaaacaaa aatggtca
//
LOCUS       DAVBCB010000130          277 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVBCB010000130 DAVBCB010000000
VERSION     DAVBCB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.130"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>277
                     /locus_tag="VQU28_002126"
     CDS             <1..>277
                     /locus_tag="VQU28_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HET2477388.1"
                     /translation="QRIIEAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAM
                     QGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKIKKKDLDISIVSQG"
BASE COUNT          126 a           37 c           47 g           67 t
ORIGIN      
        1 aacaacgcat aatagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
       61 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      121 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      181 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      241 agaaagattt ggacatttct attgtttccc aaggaga
//
LOCUS       DAVBCB010000131          272 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVBCB010000131 DAVBCB010000000
VERSION     DAVBCB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.131"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..272)
                     /locus_tag="VQU28_002127"
                     /pseudo
     CDS             complement(1..272)
                     /locus_tag="VQU28_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           75 a           72 c           48 g           77 t
ORIGIN      
        1 atacttgagg ctgtattcaa ttgcccttcc tagttttgaa cccgataaaa ctgactgacg
       61 ccggcaccaa gcaaagaaat cttccattag gttggagctc ttcttgacgt ttctgtagtc
      121 gttcatcagc tggcaaagcc tcccagtctc tttccaagaa aaataactga tcacaataag
      181 ctaaaccttt agctcctaag gatgatttat ccgcttgctt gggggtcgct tcaaaaaact
      241 tccttctcac atgcgcccaa catccaacaa gt
//
LOCUS       DAVBCB010000132          265 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVBCB010000132 DAVBCB010000000
VERSION     DAVBCB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.132"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(1..265)
                     /locus_tag="VQU28_002128"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="VQU28_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT           84 a           42 c           55 g           84 t
ORIGIN      
        1 tgaaaaagca agagctgata gagttgatag tggtgtttca agtcttccga ataactcaaa
       61 agcttgttta aaatctcttt attggttcaa tgcatacgaa aagtaggacg ataaaaatgt
      121 ttatcactca gtttacgact atcctgttga atgagtttcc agtagcgctt gatagccttg
      181 tattcatggg attttcgatg aaactgattt atgatttgaa cacgcacacg actcatagca
      241 cggctaagat gttgtacaat gtgaa
//
LOCUS       DAVBCB010000133          233 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVBCB010000133 DAVBCB010000000
VERSION     DAVBCB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.133"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            complement(<1..>233)
                     /locus_tag="VQU28_002129"
     CDS             complement(<1..>233)
                     /locus_tag="VQU28_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000927991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HET2477389.1"
                     /translation="LLFLKPLYNLLRERLLTQPLLHADETSYRVLESHSHLTYYWTFL
                     SGKVENQAITLYHHDQSRSGSVVQEFLGDYSGYV"
BASE COUNT           71 a           50 c           52 g           60 t
ORIGIN      
        1 acatagccag aataatctcc taggaattct tgtactaccg aaccactccg actctgatca
       61 tgatggtaca gcgtgattgc ttgattctca actttcccag acaaaaaggt ccaatagtag
      121 gtcagatggc tatgactctc tagaacccga taagaagttt catccgcatg aagtaagggc
      181 tgagtcaata gtctctctcg caagaggtta taaaggggct ttaggaatag gag
//
LOCUS       DAVBCB010000134          229 bp    DNA     linear   BCT 10-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain ST1
            SAMEA2066448-rid20178073.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVBCB010000134 DAVBCB010000000
VERSION     DAVBCB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA2066448
            Sequence Read Archive: ERR351762
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001980387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001980387.1
            Genome Coverage       :: 193x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/13/2023 01:24:14
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,130
            CDSs (total)                      :: 2,088
            Genes (coding)                    :: 1,949
            CDSs (with protein)               :: 1,949
            Genes (RNA)                       :: 42
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 23S)
            partial rRNAs                     :: 2 (16S)
            tRNAs                             :: 35
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 139
            CDSs (without protein)            :: 139
            Pseudo Genes (ambiguous residues) :: 0 of 139
            Pseudo Genes (frameshifted)       :: 67 of 139
            Pseudo Genes (incomplete)         :: 68 of 139
            Pseudo Genes (internal stop)      :: 37 of 139
            Pseudo Genes (multiple problems)  :: 28 of 139
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..229
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA2066448-rid20178073.denovo.134"
                     /strain="ST1"
                     /db_xref="taxon:1313"
     gene            <1..>229
                     /locus_tag="VQU28_002130"
     CDS             <1..>229
                     /locus_tag="VQU28_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HET2477390.1"
                     /translation="YTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHS
                     HWIKKDSLSEAERAAAQAYAKEKGLTPPSTDH"
BASE COUNT           72 a           49 c           55 g           53 t
ORIGIN      
        1 tacactgatg atgagattca agtagccaag ttggcaggca agtacacaac agaagacggt
       61 tatatctttg atcctcgtga tataaccagt gatgaggggg atgcctatgt aactccacat
      121 atgacccata gccactggat taaaaaagat agtttgtctg aagctgagag agcggcagcc
      181 caggcttatg ctaaagagaa aggtttgacc cctccttcga cagaccatc
//
